LOCUS       DAWFJW010000001        50626 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFJW010000001 DAWFJW010000000
VERSION     DAWFJW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 50626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.001"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(67..3166)
                     /locus_tag="WGR19_000001"
                     /pseudo
     CDS             complement(67..3166)
                     /locus_tag="WGR19_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(3203..3306)
                     /locus_tag="WGR19_000002"
                     /pseudo
     CDS             complement(3203..3306)
                     /locus_tag="WGR19_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3351..3932)
                     /locus_tag="WGR19_000003"
     CDS             complement(3351..3932)
                     /locus_tag="WGR19_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0797615.1"
                     /translation="MGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGRILDNS
                     LKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALD
                     INFAFYYFFIIAEQSKKLINTSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTL
                     THSISEGLPHEIALRRKQYEYYREQLLAFPKAT"
     gene            complement(3973..4569)
                     /locus_tag="WGR19_000004"
     CDS             complement(3973..4569)
                     /locus_tag="WGR19_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0797616.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGDS"
     gene            complement(4735..5745)
                     /locus_tag="WGR19_000005"
     CDS             complement(4735..5745)
                     /locus_tag="WGR19_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0797617.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(5742..7286)
                     /locus_tag="WGR19_000006"
     CDS             complement(5742..7286)
                     /locus_tag="WGR19_000006"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0797618.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTSIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(7359..8363)
                     /gene="rfaD"
                     /locus_tag="WGR19_000007"
     CDS             complement(7359..8363)
                     /gene="rfaD"
                     /locus_tag="WGR19_000007"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0797619.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(8404..9522)
                     /locus_tag="WGR19_000008"
     CDS             complement(8404..9522)
                     /locus_tag="WGR19_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0797620.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNRLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(9524..10759)
                     /gene="dcm"
                     /locus_tag="WGR19_000009"
     CDS             complement(9524..10759)
                     /gene="dcm"
                     /locus_tag="WGR19_000009"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0797621.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(10796..11767)
                     /gene="hldA"
                     /locus_tag="WGR19_000010"
     CDS             complement(10796..11767)
                     /gene="hldA"
                     /locus_tag="WGR19_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0797622.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(11823..12563)
                     /gene="pyrF"
                     /locus_tag="WGR19_000011"
     CDS             complement(11823..12563)
                     /gene="pyrF"
                     /locus_tag="WGR19_000011"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0797623.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPLAVLREVNRVANLEAN"
     gene            complement(13089..13736)
                     /gene="adk"
                     /locus_tag="WGR19_000012"
     CDS             complement(13089..13736)
                     /gene="adk"
                     /locus_tag="WGR19_000012"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0797624.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            13951..14790
                     /gene="htpX"
                     /locus_tag="WGR19_000013"
     CDS             13951..14790
                     /gene="htpX"
                     /locus_tag="WGR19_000013"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0797625.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGNLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(15091..15651)
                     /locus_tag="WGR19_000014"
     CDS             complement(15091..15651)
                     /locus_tag="WGR19_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797626.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(15765..16157)
                     /locus_tag="WGR19_000015"
     CDS             complement(15765..16157)
                     /locus_tag="WGR19_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0797627.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(16365..16757)
                     /locus_tag="WGR19_000016"
     CDS             complement(16365..16757)
                     /locus_tag="WGR19_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0797628.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(16874..17284)
                     /locus_tag="WGR19_000017"
     CDS             complement(16874..17284)
                     /locus_tag="WGR19_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0797629.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(17298..17735)
                     /locus_tag="WGR19_000018"
     CDS             complement(17298..17735)
                     /locus_tag="WGR19_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0797630.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(18147..19445)
                     /locus_tag="WGR19_000019"
     CDS             complement(18147..19445)
                     /locus_tag="WGR19_000019"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0797631.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAESVKAEDVMAVIEKVAPRITPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(19548..20699)
                     /locus_tag="WGR19_000020"
     CDS             complement(19548..20699)
                     /locus_tag="WGR19_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0797632.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            21034..22413
                     /gene="norM"
                     /locus_tag="WGR19_000021"
     CDS             21034..22413
                     /gene="norM"
                     /locus_tag="WGR19_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0797633.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            22457..23497
                     /gene="murB"
                     /locus_tag="WGR19_000022"
     CDS             22457..23497
                     /gene="murB"
                     /locus_tag="WGR19_000022"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0797634.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHGRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            23509..24159
                     /locus_tag="WGR19_000023"
     CDS             23509..24159
                     /locus_tag="WGR19_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0797635.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            24214..24990
                     /locus_tag="WGR19_000024"
     CDS             24214..24990
                     /locus_tag="WGR19_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0797636.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECDETALPDPQTESVRQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNILGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFFTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            25041..25607
                     /locus_tag="WGR19_000025"
     CDS             25041..25607
                     /locus_tag="WGR19_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0797637.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAAQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            25627..26517
                     /locus_tag="WGR19_000026"
     CDS             25627..26517
                     /locus_tag="WGR19_000026"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0797638.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            26542..27300
                     /locus_tag="WGR19_000027"
     CDS             26542..27300
                     /locus_tag="WGR19_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0797639.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(27355..28020)
                     /locus_tag="WGR19_000028"
     CDS             complement(27355..28020)
                     /locus_tag="WGR19_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0797640.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(28134..29030)
                     /locus_tag="WGR19_000029"
     CDS             complement(28134..29030)
                     /locus_tag="WGR19_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0797641.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            29099..30256
                     /locus_tag="WGR19_000030"
     CDS             29099..30256
                     /locus_tag="WGR19_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0797642.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFY
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(30319..31320)
                     /gene="hemB"
                     /locus_tag="WGR19_000031"
     CDS             complement(30319..31320)
                     /gene="hemB"
                     /locus_tag="WGR19_000031"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0797643.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(31503..31787)
                     /gene="yhbY"
                     /locus_tag="WGR19_000032"
     CDS             complement(31503..31787)
                     /gene="yhbY"
                     /locus_tag="WGR19_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0797644.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            31897..32517
                     /locus_tag="WGR19_000033"
     CDS             31897..32517
                     /locus_tag="WGR19_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0797645.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            32581..34548
                     /gene="ftsH"
                     /locus_tag="WGR19_000034"
     CDS             32581..34548
                     /gene="ftsH"
                     /locus_tag="WGR19_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0797646.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            34770..35201
                     /locus_tag="WGR19_000035"
     CDS             34770..35201
                     /locus_tag="WGR19_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0797647.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            35194..35472
                     /locus_tag="WGR19_000036"
     CDS             35194..35472
                     /locus_tag="WGR19_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0797648.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            35525..36103
                     /gene="pth"
                     /locus_tag="WGR19_000037"
     CDS             35525..36103
                     /gene="pth"
                     /locus_tag="WGR19_000037"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0797649.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            36164..36370
                     /locus_tag="WGR19_000038"
     CDS             36164..36370
                     /locus_tag="WGR19_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0797650.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            36367..36729
                     /locus_tag="WGR19_000039"
     CDS             36367..36729
                     /locus_tag="WGR19_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0797651.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            36960..38375
                     /locus_tag="WGR19_000040"
     CDS             36960..38375
                     /locus_tag="WGR19_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0797652.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKIAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            38494..39003
                     /locus_tag="WGR19_000041"
     CDS             38494..39003
                     /locus_tag="WGR19_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0797653.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKHHGYHDDVPSEP
                     VVIIKAEAV"
     gene            39166..40548
                     /locus_tag="WGR19_000042"
     CDS             39166..40548
                     /locus_tag="WGR19_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0797654.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYHNHI
                     VGHVKLKRPMNIAIDAGNGVGGAFAGKLYRALRCGVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSSMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(40568..41323)
                     /locus_tag="WGR19_000043"
     CDS             complement(40568..41323)
                     /locus_tag="WGR19_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0797655.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(41333..42049)
                     /locus_tag="WGR19_000044"
     CDS             complement(41333..42049)
                     /locus_tag="WGR19_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0797656.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAIASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(42039..42866)
                     /locus_tag="WGR19_000045"
     CDS             complement(42039..42866)
                     /locus_tag="WGR19_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0797657.1"
                     /translation="MMLKKIVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            43151..44059
                     /locus_tag="WGR19_000046"
     CDS             43151..44059
                     /locus_tag="WGR19_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0797658.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            44139..47753
                     /gene="recB"
                     /locus_tag="WGR19_000047"
     CDS             44139..47753
                     /gene="recB"
                     /locus_tag="WGR19_000047"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0797659.1"
                     /translation="MSAPIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAALKKGKTPDAAAFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAGDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGTDGKGGGVWRWDIDAAALEQIK
                     "
     gene            47792..48151
                     /locus_tag="WGR19_000048"
     CDS             47792..48151
                     /locus_tag="WGR19_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0797660.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            48283..48762
                     /locus_tag="WGR19_000049"
     CDS             48283..48762
                     /locus_tag="WGR19_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0797661.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTIGE"
     gene            complement(49038..50417)
                     /gene="radA"
                     /locus_tag="WGR19_000050"
     CDS             complement(49038..50417)
                     /gene="radA"
                     /locus_tag="WGR19_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0797662.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNVKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
BASE COUNT        11997 a        13922 c        12494 g        12213 t
ORIGIN      
        1 tccactataa aaaccgcctc cgcataaaat caaaaatgcc gtctgaaagg ttcagacggc
       61 attttgtcaa atatcggtcc ctattccggc aaacttctcc acaaacgccg caatcttttg
      121 aaaaacactt tgtttcttcg tcagatattg cgggttgagc ggactcattt tcggcagggt
      181 ttcggtcagt tccgtgccgt tttcgctggc atagccgcgt ttgagcgagc cggtcaaata
      241 gcgtttggcg gcggtttcgt tcaggccttc ggcgacaatc aatcctgccg cttcgtgccg
      301 catcacctct tgcgcgtagg tgtagaaggt ttccagtatg gcgggaacgt cgggtacttt
      361 gtccaaatcc gtatcgttga tgaaatccac aatcagaccc tctttggcgc ggtggccgat
      421 gctggcgcgg atgatgcggc ggatttcttc caccagctcc gctttgcctt tgattttttt
      481 gtggtgttcg aaaaccagtt gcaggatgta atccagattg atttcctgtg atttgagcaa
      541 atccacctca aaaaccacat cgtcccaatc gatttttgac tgttctttct gttcgcctgc
      601 tttttggcgg cgcaaccaat cgcggatgtc gttgtaggcg gaacggtagt cctgcaccgc
      661 cctttcagac ggtatcggca cttgccgcat ttcctgcacg tcttcatcgc tcaagtagta
      721 tttttcttga aacgccttca tcgcatcttc gtccgccgcg tccacactct gcaactcgcg
      781 cagcgcggca aattcatcgt agttctgcaa tacgttttcc gcccgcaggt attcgccgaa
      841 gagtttggca aaatcttttt tgtctttttc cgtttcgatt tcgtcgggtt cggggaaacg
      901 ttcacgcaac tcttttgcca catccaaata accgcgccgc gcttcgccgg tctggctgtc
      961 ggtatagccg ttcatgtatt cttcgtagct tttttccagc accacgtttt tggtgtgttt
     1021 gtcgccgaac agggtaatcg catcaatggt tgcctgctcc aaatcgcgga agcagacaat
     1081 attgccgaag gttttggtgg catcgtaaat gcggttggtg cgcgaaaacg cctgcatcag
     1141 gccgtgatag cgcaggtttt tatcgacgaa cagcgtgttc agcgtcggcg cgtcaaaacc
     1201 cgtcaaaaac atgccgacca caatcagcaa atccacttcc tgatttttca cccgttttgc
     1261 caaatcgcgg tagtagtttt gaaaggcttt gctgtccgtg ccgaaattgg ttttgaaaca
     1321 ggcgttgtaa tcgttgatgg cagcctgcaa aaattctttc gcgctgccgt ccatcgcttc
     1381 cggttcaaag gtctcatcaa caatttcacc gacggcgttc tgctcttcgt tggccgcaaa
     1441 ggaaaaaatg gtggccactt tcagcgggtg caggctgcct gcctgttgtg ttttgaacgc
     1501 ttcgtaatag cacttcgccg catccacgct gctgaccgca aacatggcgt tgaagccttt
     1561 accgcccgca ttcaggcggt gcgttttctg cctgaactgg ttcaggatat attgcgtgat
     1621 ttcgcggatg cgttcggggt gcagcagggc ttggcggttt tcggcagcac tcagtttctt
     1681 ctcatcctgt tccgcttcca cggctttgaa ctgcgggcgc acatcgttgt aatccacttt
     1741 gaatttcaat actttttcat cgcggatggc atcggtaatc acataagaat gcaactcacg
     1801 cccgaacacg cccgccgtgg tttccgcgcc caaagcgttt tcgggaaaaa tcggcgtacc
     1861 ggtaaagccg aactggcaga attttttaaa tttctttttc agattttttt gcgcttcgcc
     1921 gaattgcgag cggtggcatt cgtcgaaaat aaagacaacc tgctggtgat aaaccggcag
     1981 attatcctca ctcttcatca ggttgttcag cttttggatg gtggtaacga tgattttgtt
     2041 gtcgtctttt tccaaattgc gtttcaaacc cgccgtgctt tccgaaccgt ttacgctgtc
     2101 gggcgaaaaa cgctggtatt ccttcatcgt ctggtagtcc aaatccttcc tgtccaccac
     2161 gaagaaaacc ttgtcgataa acgccgattc cgtcgccaga cgcgccgcct taaagctggt
     2221 cagcgttttg ccgctgcccg tggtatgcca aacatagccg ccgctttccg gtttgctcca
     2281 attcttcgcc tgcgccgaac tgttaatttt ccacagaatg cgttcggcgg cggcaatctg
     2341 gtacggccgc ataatcagca gcgtatcatt cgcatcgaac acgctgtaat gcagcaaaac
     2401 gctcagtaat acgctttttt tgcaggaacg tggcggtaaa gtctttcaaa tccttaatcg
     2461 gatgattgtc cgaccgcgcc caattcatcg tgaaatcgaa gctgtttttg tcgcgcttgg
     2521 tggtattggc gaaatagcgc gtgtccgtgc cgttggaaat cacgaagatt tgcaggaatt
     2581 tgaacagcga attttcgctg ttgaagctct ctttgctgta acggtgcacc tgattgaatg
     2641 cctcgcgcac cgccacaccg cgctttttca attcaatctg caccagcggc aggccgttta
     2701 ccaacacggt aacgtcatag cggtttgcat gcgtgcccgt ctgctcaaac tggttgataa
     2761 gctgcacatg gttgcgggca aggtttttct tgtccagcag ataaatgttt ttcagacggc
     2821 cgtcatcaaa agcgaaatca tagatatggt cgtcgtggat tttgcgggtt ttatcggtaa
     2881 tgttttcaga cggcctgtcc agatattccg tcaaaaaccg cgcccattcg ccgtctgaaa
     2941 acgccacatc gttcagccgc tgcaactgcg cgcgcaggtt ttccagcagc ctgctttggc
     3001 tgttcaaatc cttgcggtat tcgtaaccct gattctgcaa atcggcgatt aactccgctt
     3061 ccaactggtt ttccgattgg tagctgcccg actgttcgat tttttcatat tggtcgagca
     3121 cgatgaaatt cggcgtttcg gcgatgggtt tggtttcgag gttcatatgg gtcgtccttt
     3181 gcggtgtcgg gttttatagt gaattaaatt taaaccggta ccgcgttggc tcgccttgcc
     3241 gtactatttg tactgtctgc ggctcgccgc cttgtcctga tttaaattta attcactata
     3301 aacggtttga tgttcggatt gggctgcctg aaaatgtgca ggctgctttt tcaggtagcc
     3361 ttagggaagg caagcagctg ttcgcggtaa tattcgtatt gtttgcggcg cagggcgatt
     3421 tcgtgcggca ggccttcgct gatagagtgg gtcagggtat cgaatttgtc taggatggcg
     3481 gcaatttttt cctgttcggg gagtggtggg atggggattt ttaatttctt taattctttc
     3541 atgctaataa taggtaaact tgaagtatta ataagttttt tgctctgctc cgcaataatg
     3601 aaaaaataat agaaagcgaa atttatatct aaagcatcct taaattcatc tttaattgta
     3661 aaattggtca gttgttggtt actaatataa ttaactttta ttaacgcatg ctctcctatg
     3721 gttgccgttg tcgacatcat tattgattta gcaggaaaca gcttgccgcc cttaactgcg
     3781 ctttttgata tatgcttcaa tgaattatct aaaatacggc cattttccct aatatcttcc
     3841 attctaaacc aagggattga gccattttcc caatattctt tatttgattt ggaaggggta
     3901 tagccattct tcaagtcaaa cacctccccc aacgtcttcc aaaccacatc tttcagacgg
     3961 cctttatagc catcagctat cccccccgat ttgattgtca aaatctaaaa gaaagtcgcg
     4021 gtaataccgg tattggcgtt tgcgcagggc taattcagct tccagctcgg tgaatttgtc
     4081 aagtattttt acaatttttt gttgggtttc caggggtggg atagggatta ataatttttt
     4141 caagttactg gcatttagag caggaatacc tgtaaatgta cataaggcat gaatctcttg
     4201 ttcaaattca agtaatatat gatagagaaa acgaacatcc aattctgttc tattcttaat
     4261 agtaactgca taatttaagt tgccacgaaa atatcttcct tcttgccacg ttattgagcc
     4321 gactcccgct cctcttgttg tgatgccgat agggtcattt tcagtgttcc attcatcaat
     4381 atagccaagc ggctctttgc cactgttaat aactggataa ctaccaatat tatttgagat
     4441 tttctgctta cttacagctt gtcctgtttt tatgatagct atcccatttt ctccacccaa
     4501 cggcttccac tccaccggtg ccgtctgaat catctcaatc aattttttcg ctttgctttg
     4561 catatccatg gttgttttcc gtttctttcc ctctgcggcg ggatgcgggg cggttgtggg
     4621 gtgcttcggc tcactctgtc cgtttgccgt tatttctgcg ggctgaagcc cgtcctgctt
     4681 tcaggctgcc tgcaatggtg tgcaggctgc tttttcaggt agcctgaatg gtagttattt
     4741 tgtgcggtct tttttctgtt tgatctgttg cacggctgtt ttttccaacg ctttaacgct
     4801 ttccagatag gcttgctcca ccggcgacag gatgcgcgct tcataggcgc ggtattcgcg
     4861 ttcggctttc tgctctgcct gtttgcggct gatgctgcct gcaccctgta atgtgccttg
     4921 tccgtacagc ccggaaaatt tgtccaattc ggctatccag tcgcgcatat acatggggct
     4981 ttgctcctgc gctttgattt ccgccaggtc gaagaaggcg gaaaccagac ggttcaggcg
     5041 gaacagttcg tcttcggtca gatagttttt ggcgattttg gcttcattca gcgtggggat
     5101 tgcgccttga aaggtggtca gccccataaa gtctttgctg ctgtcggcac ggctgtatat
     5161 cagctcagct gcggtttgcc ggctggcggc atagtgcagt ttgttttgaa cggcggcaaa
     5221 aaaggtttgg ctttcgctgc ttttggggtt gtagtcttgg ctggtggcat ataaatcaag
     5281 cacttgccgg tatagggctt tttcgctgct gcggatgtcg cgaatgcggt tgagcagttc
     5341 tttccaatag tcgccgccgc ctgtgccttt caggcgttcg tcgtctatgg caaagccttt
     5401 ggtcagatat tcgtccagcc gttcggttgc ccattggcgg aattggatgc cccgcgcgga
     5461 acggacgcgg tagccgacgg caataatcat gggcagggaa taatgggcga ttttgcggtt
     5521 taccttgcgc ccgttttcat tttgaactgt caactggaag ttgacagttg ccttctcttc
     5581 caattcttgc tctgcaagaa tggttttaat gtgtttgctg atattttgtt tggtggtttg
     5641 gtacagttct gccatgtccg cctgcgtcag ccaaagctgt ccgccaaatt cctgcaaggc
     5701 aaattgggca gtgccgtcgt tggcggtgta taggatgatg ctcatgcttc aatctctgca
     5761 atcacttcgt caatttcacg ccgcagccgt tcgattttgg caacggtttc gctgatttcg
     5821 gcgttaagct gtttgatgtc gataatttcg cgggtgtctt cgggttcaac atagctgctg
     5881 acggcgaggt tgtagccgtt gtctttgacg gtttgctggg cggcgttttg ggcgatatgc
     5941 ggcacatcgg ctttatcggc gaagagtttg acgatttcgg caatgtgttc ttcggttaag
     6001 acgttgttgt tggtttcttt tttaaagaag ccgcctgcgt cgatgaattg gatgtcggta
     6061 ttgtctttgt gtttggacaa aaccaggata ttgacggcga tgctggtgcc gtaaaagaga
     6121 ttgggcgcaa gggcgatgac ggtttccacg tagttgccct ccaccagata ttggcggatt
     6181 ttctgctctg cgccgccgcg atagaaaatg ccggggaatg agacgatggc ggcgcggcct
     6241 ctgccggaaa ggtagttcag tgcgtgcaga atgaaggcaa aatcggcttt ggatttcggg
     6301 gcgagtacgc ctgcaggggc aaagcggtcg tcgttgatta aggtggggtc gccgctgcct
     6361 atccagttga tggaataggg cggattggaa acgacggcat caaagggttt gctgtctttg
     6421 agtttgggat tggtcagtgt gtcgcccaat tcgatgtgga atttgttgta attgacgttg
     6481 tgcagaaaca tattcatgcg ggcgaggttg taggtggtgt ggttgatttc ctgcccgaag
     6541 aagccttctt cgatgatgtg ctcgtcaaac tgttttttcg cctgcaagag caggctgccc
     6601 gagccgcaag ctgggtcgta gattttgttt actttctcct gcccgtgcac cgccagccgc
     6661 gcaatcagct tggatacgct ttgcggggtg aaaaattcgc cgccggattt gcctgcgttg
     6721 gcagcgtagt tggaaatcag gtattcgtag gcatcgccga aaaggtcgat gtggtggtct
     6781 tcaaaactgc cgaaatcgag ttccgccacg ccttttagga cggcggcaag gcgtttgttc
     6841 ttgtcggcaa cggtgctgcc gagccggctg ctggtggtgt cgaagtcgtc aaacaggcct
     6901 ttaatgtctt gttcggacgg atagccggag gcggagcttt caatcgcggt aaaaatttct
     6961 ttcagcttgg tgttgagctc ttcgttttga tgggcttcgg cggcaatatt gcaaaaaagc
     7021 tggccggggt agatgaaata gcctttgact ttgacggcat cgtctttgat ttcgggcgtg
     7081 atgatgctgt ccggcatagc ggcgtaatca atactgctgt cgcctgcctg catatagtcg
     7141 gtaaagtttt cgctgataaa gcggtagaaa agtgtgccga gaacgtattg tttaaagtcc
     7201 cagccgtcca ccgcgccgcg tacttcgtcg gcaattttcc aaatttggcg gtgcagttgg
     7261 gcgcgttgtt gcatttcggt catcatcgaa atccatataa aagttaaaca aatcgaaatc
     7321 gcctgatatt ttcagacgat tttttacggg cattcaaatt aagccaaatt ttccagcatc
     7381 catttgacgt agcgctcgac gcctgatttg acatcgaaaa attcttccgt ataacctgcc
     7441 tcacgcaatt tggtgatgtc ggcttgagtg aagctttggt atttgccttt gagcgcatcg
     7501 ggaaatggga tgtagcggat aagttcttct tctaccagct ctttcaagct catttcgggt
     7561 ttgccttcgg cggcgcggca ggcgttgacg gtggcggcgg cgagttcgtt aaactgttgg
     7621 ctgcggccgg tgccgaggtt gtagatgccg gaaagttcgg gatggtcgaa gaagtagagg
     7681 ttgactttgg caacgtcttc gacgctgaca aagtcgcggg tttgttcgcc gttgccgtag
     7741 ccgtcgttgc tgccgaacag gttgacgtaa ccgtgttcgc ggtattggtg gaaatggtgg
     7801 aaggcgacgg atgccatacg gcctttgtgt tgttcgtgtt gtccgtacac gttgaagtag
     7861 cggaagccga cgacttgggc ggtcaagcct tctttcatgc ggcggcgcaa tacttggtca
     7921 aacaggaatt tggaatagcc gtacacattg agcggttttt cgagttcgcg ctcttcacgg
     7981 aagatttcgc ctttaccata cacggcggcg ctggaggcgt agaggaaggg aatgcgttcg
     8041 tcctgacacc agtccaacaa atccagcgtg tactggtagt tgttttccat catatacaaa
     8101 ccgtcgtggt tcatcgtatc ggaacacgca ccttgatgga acacggcttc gatattttga
     8161 taaggtaaaa tgtgttccct cacttggcgg ataaattcgt gtttgtcgag gtagtgggcg
     8221 atttcgcact cggcaaggtt tttgaatttt tcgcctttgc tcaaattatc gacggcaaca
     8281 atgtcagtaa taccgcgttg attaagtgct ttgacgatgt tgctgccgat aaagccggcc
     8341 gcgcctgtta caatgatggt catattcggt ttcctttgtt ttgagtaaaa ttaataaact
     8401 tagttacaag aaatcatcga tattgtcctg aaacacaaat ttgcgatgat attccaaata
     8461 tttttctctt ccttcgggat atattctcat cttatttttt gcatttggat tgatattaag
     8521 tttttcccat gtgtaacggc ttagtcgagt accgcaaatc aaacgaccat catccaagaa
     8581 agtaatataa ccttgatcga acaaccaatc ataagtcggc gttaaagcta acctatttaa
     8641 ataatctaat gcctctttct catttttctc agtaatacaa accatataag gtttaatatg
     8701 actagccctt aacaaaatct catctgtaat taatgtaaat ggacattgcg gcatatgatt
     8761 tataacatcc ttgcgatatt tctgctgccc ttttctactt ttctgtttaa tgatttcttg
     8821 ctctacgaat tgattaaatt ctgaagccgg tatttctaat ttttctcttg ataagagttg
     8881 cgggtgcacg atagagcgaa attgataatc caaaaatatt cggaaataaa ataatggttc
     8941 tgaatcttct atatctttga caggcaatag tttaagaatt gataaataac taattttggg
     9001 caatacaatc ttacgccaaa ttgaccatat atcatcctcc gaacggatat aacccctacg
     9061 gtaaccttta gaattttcca aatggtctga tacatcatgg attgaaaaga aaatattttc
     9121 tgtaggcaga ttattaatac ttaaaacgta atcatcataa aaccttgaaa tatcatgatg
     9181 atatttctgt tcttgtttgt gatattcaac ttttgcatca accaaataac gctgtaaatt
     9241 tttcttatta aaaaaacaat ttttctgaat gagaggatat tctaccctcc ttccattttt
     9301 tgtctcagta atcccccaat tttcatactc aaaaaatgta cttagttttc gaccactatc
     9361 atcaatataa ctacctacat atttgcgtgc ttccccatta ccaccaaaaa cagataactt
     9421 gttattcctg tgaataaaac tatcttccgc acgtaaattt tgaatactat cgacaataaa
     9481 atactctatg ttttcaatag taattctttg aaaatccggc attttactct ccataaagcg
     9541 taatatccat ttgttttaat atagcgatta atacatcaac aacaatactg tttcccgctt
     9601 ggcgatacat ttgagtatca ctaactaaaa ttttaaaatc atctctaaaa cccattagcc
     9661 gcaaacattc cctaggtgtt aatttacgaa tacgtccacg attatgagta acataattat
     9721 caaccccggc acgatgcatt ttatgcatag tttgcaataa tgggcgagct acaggcaaat
     9781 cagtttccgt actggtttta aaattttttg ttcctcctgc caaaacataa tttttgattt
     9841 tttcagaaag ataatatttt tcctcaacgt tatttacatc aaaaataaag tcatcaaatt
     9901 cagattcgcg ggctgcctga aaaataaaat caccatgcca attaaattgt tgatttgctt
     9961 tttggcataa ggcaatttct ccattaatct gtgtataacg tttttgtcta tttttagaac
    10021 tggtaacaaa tttcacaccc ttttcacgta aaaaatattt gctatcagta tagtcctcca
    10081 aaaaatcttg catagtatgt tctaattcaa tcttttcagg aaactgaaaa ccatttatag
    10141 gaggggtatg aaagccaaca acaaaaatac gctcacgatg ttgaggaatc ccataatcct
    10201 tactattcat tatttggaaa tataagtcat aaccaagtga ataaaaaaca ctttttacaa
    10261 ctttccaagt ttttccatta tcatgattga gcaagccctt tacattttca taaataaata
    10321 tctttggttg gacttcatcc acaacgcgag caaattcata gaataatgtt cctcgtgtat
    10381 cttctaatcc tgcacgtttg ccaaccatgg aaaatgcttg gcaaggactg cctccaacta
    10441 aaatatcaac ttgatttcta aactttctcg catcaaattg agtaatgtcg ttatgccaaa
    10501 aatcttcatt taatttatag tttccaagat aacttttttt aacgtatgga tcaatatctc
    10561 ctgcaaaaac aatggtatgg tttaaattta atcggtggaa tgcctgttca accgcaccaa
    10621 tcccgctgaa tacggttgct aatctaatat gtgaatcagg tttaagaaaa gttttagatt
    10681 tccaaccttg ttgactggga aagagcaaag ttttttgtaa tcgagtatcg tgtgtctgtg
    10741 ccattgtcga aatagtcata cttatatcgt tctgtttatc ttatcaatat gaaaactaca
    10801 ttgttgattg ccctgacaat gccttgatca attcggcaaa cgagcaaacc gccgtaccga
    10861 gtttcgccac gacaaccccg gccgcagtat tggcaaggta catggcttcg ggcatggtgc
    10921 agcctgccgc caaacccaag cccattccgg caatgacggt gtcgcccgca ccggatacgt
    10981 cgtaaacttc ttgggcgcgg gtgggctggt aaatcggttc gccttcgctg aacaaggtca
    11041 tgccttcttc gcttcgggtc agtaaaacgg cggtcaggtc gaggtggcgg cgcaggtttt
    11101 gcgctttttc ggtcagctcg ctttcgtttt tccaactgcc gaccacttct ttcaattcgg
    11161 cgcggttagg cgtaatcaga gttgcaccga catatttttc gtaatcgtcg cctttggggt
    11221 cgattaagac ggttttgccg gcgtgtttcg cccaatcgat catatcggag atatgcgaca
    11281 ggccgccttt gccgtagtct gaaaaaatga ttgcgtcgta ttcgggcaag atttcgcggt
    11341 atttctgctt gatttgttcc aacacttcgc agttgggatg ttcttcaaaa tcaagacgga
    11401 taagctgctg gttgcgggcg acgacgcgca gtttgacggt ggtggcgatt tgtttgtcgc
    11461 gcatcagata ggaggcgacg ccgtcctgca ccatcagcgc atcgagcgcg tcggcggctt
    11521 cgtcgttgcc ggttacggac aacagccctg ccctgccgcc caacgaagcg atgttgcgcg
    11581 cgacatttgc cgctccgccc gcgcgttggt cgattcgtcc gatttttgcc accggcacgg
    11641 gggcttcggg cgaaatacgg gacacatcgc cgaaccaata gcggtcgagc atcacgtcgc
    11701 cgacaaccag gactttggct tgcgcgaaac gggatttgag ggtttcttgt tggaacttgg
    11761 cggacatatt gcctccgttg tgttttgttc agacgacctt aaccggtaag gccgcctgaa
    11821 aatcagtttg cttcaaggtt tgccacgcgg ttcacttcgc gcaatacggc tagcggatcg
    11881 gcagcttggg taacgggacg gcccatcacc agataggtcg aacctgccgc caaggcttcg
    11941 gcgggcgtca tgatgcggcg ttggtcgtcg ttgttgccgg caacgtccaa gcggataccg
    12001 ggcgtgacca agacaaaatc ctgtcccaat tcgcggcgca gcggtgcggc ttcttgggcg
    12061 gagcagacca cgccgtccaa gcccgaactt tgcgccagtt tcgccaaacg gatgacttgt
    12121 tcttcagggg cggtgttcaa accgatttcc gccaaatcgc tttgttccat gctggtcaac
    12181 acggttacgc cgattaagag cggcttcgtg ccgtatccgg caacggcttc tgctgcggct
    12241 tccatcatac ggcggccgcc cgatgcgtgc atatcgacca tccaaacgcc catgtcggca
    12301 gcgactttgc aggcttgcgc gacggtgtgg ggaatatcgt ggtatttcaa atcgaggaaa
    12361 agtttgaaac cttgattgat taagctttct gccaaattgc gtcccgtcgc ggtaaacagc
    12421 tctttgccga ttttgatttg acacaatgtc gggtcaaggt tgcggacgaa tccgagcgtg
    12481 tctttttcgt tggcaaaatc aagggcgacg atgacggggg tgcgttgttg cggagtttgg
    12541 aagtcggaga ttaaaggatt catggtgttt tcgtttcggg ttttcagacg acctttatcg
    12601 tctttttcac agtatagtgg attaacttta aaccggtacg gcgttgtctc gccttagttc
    12661 aaagagaacg cttctctaag gtgctgaagc acaaagtgaa tcggttccgt actatctgta
    12721 ctgcctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataac aacgtaaatt
    12781 caaaccgtag gtcgggcatt gatgtccgac ctacgcggac tcaagccccc gacaaactgt
    12841 ttcggataaa tttgaaccag tacatcagcc gtcattcccg cgcaggcggg aatccattga
    12901 taaaactcag ttcatcagat ttaaacagcg attgcccaaa ttcagaaatg gattcccgcc
    12961 tgcgcgggaa tgacgccaaa tgattatttt tcggttgcaa cacgctgttt atcaaaaaca
    13021 ggtcgtctga aaacctgatt ttctgttttc agagtgcgaa gcctgcccgt gggtacgacc
    13081 ttttcgattt atttgcccaa tgcgcccaat acttcggctt tcacggcttc taccggctga
    13141 gtgccgtcaa ctttgatgta tttcggcgcg tgttcgcctt ccagtttgct gtaaaaatcg
    13201 accaaaactt cggtttgctc gtggtaaacg gcaaggcgtt ttttgacggt ttcttctttg
    13261 tcgtcgtcgc gctgaatcaa atcttcgccg gttacgtcat ctttgccttc aactttgggc
    13321 gggttgtagg taacgtggta agtacggccg gaagccaaat gcacgcggcg gccgctcata
    13381 cgatcgacaa tcacgctgtc aggcacgtcg atttcaacga ctgcatccaa atccacgcct
    13441 gcttcaacca tcgcttcggc ttgtgccaat gtgcgcggga aaccgtcgaa caggaaaccg
    13501 tttttgcagt cgtcttgcgc gatgcgttct ttgaccatac cgatgatgat gtcgtcgcgc
    13561 accaagccgc cttcatcaat gatttttttc gcttccaaac ccagcggcgt gcctgcttta
    13621 atcgcagcgc ggagcatatc gccggtagag atttgcggaa tgccgaacgc ggcggtgatg
    13681 aattgcgcct gagtgccttt gcccgcgccc ggcgcgccta aaagtaatgc tttcatgaag
    13741 tgatcctttg aaatgtgttt attggaatca aacagatggt ttacgattct acacggcttg
    13801 gcacaatagt tcaaaaacca actgcacaaa ctgccatttg caagcaaatg ttttgcatac
    13861 ctttgccgag tctgatattc acaagccggc aactttttgg gacaatcccc aaaatttcaa
    13921 aacatcacac cctaccaaaa aggaacatcc gtgaaacgaa tttttctgtt tttggctacc
    13981 aatatcgctg ttttggtcgt aatcaacatt gttttggcgg ttctgggcat caacagccga
    14041 ggcggtgcgg gcaacctgtt ggcgtattcc gccgtcgtcg gcttcactgg ttcgattatt
    14101 tcgctgctga tgtccaaatt catcgccaaa caatcggtcg gcgcggaagt tatcgacacg
    14161 ccgcgcaccg aagaagaagc ttggcttttg aacactgtcg aagcccaagc gcggcaatgg
    14221 aatctgaaaa cgcccgaagt cgccatctac cactcccccg aacccaatgc ctttgccacg
    14281 ggcgcatcga gaaacagctc cctgatcgcc gtcagcaccg gtttgctcga ccatatgacg
    14341 cgcgacgaag tggaagccgt attggcgcac gaaatggcac acgtcggcaa cggcgatatg
    14401 gttacgctga cgctgattca aggcgtggtc aataccttcg tcgtgttcct gtcgcgcatt
    14461 attgccaacc tgattgcccg aaacaacgac ggcagccagt cccagggaac ttatttcctg
    14521 gtcagcatgg tattccaaat cctgttcggc ttccttgcca gcttaattgt catgtggttc
    14581 agccgacaac gcgaataccg cgccgatgcg ggcgcggcaa aactggtcgg cgcgccgaaa
    14641 atgatttccg ccctgcaaag gctcaaaggc aacccggtcg atttgcccga agaaatgaac
    14701 gcaatgggca tcgccggtga tacgcgcgac tccctgctca gcacccaccc ttcgctggac
    14761 aaccgaatcg cccgcctcaa atcgctttaa accgatttga aacggcaaaa accgtacgcg
    14821 caagcagtac ggttttttgt atatgctgtc tgaaaacatc aggtttccat accgacacca
    14881 accgtatcca tttccgcacc ggatacgccg cattgatata ccctgataaa ccgtatccgg
    14941 acagaaaata tagtggatta acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga
    15001 aaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat ttatactgtc
    15061 tgcggcttcg tcgccttgtc ctgatttttg ttaatcccct atatcagata aaacagcggt
    15121 aaaattgccg tctgaaacca actctgcgcc atacgggaca cgctctgcac gtctatttgc
    15181 ctccgatata tcctccctac tgtaggaacg tctgccgatt tgcacgaaat cggcacctct
    15241 agcacccttc aggatggcgg acgttatccg ttcttgggat tctgcaagac ggtcgacggc
    15301 attatacaga cgctcggtct gatacccttc cgaagcaacc tgcttcaggt attccaaata
    15361 accgtaaccc aacgcccctc cgacaagcag ggtcgtcaac gctaccagcg ttgccgaacg
    15421 gattttaggg ggcatatctt gaagcatatc tctaccgaac cgaaccaaag tctctttaaa
    15481 gtcggactct acacgcgtag aaccctcgga aatcttgaag ttcaactcat actgcttccg
    15541 tttgtccgct gtcaaccgct tgaggttgtc atccccgtat tggacggttg acacaaccct
    15601 gtacagactg tcctgtattt cgaccagcgt ctgtgcaaag ttggcggtca taactggatt
    15661 cccaaccctt gccgccgatt ctgatactca aaacggtatc taggcgttcc agcagggctt
    15721 cgggagacag cagtgcgtat tcttcaatat tttccatagg ttgattatag cagaacctta
    15781 tacctgttac agacaaggtt gtcgggattt gaacgtccgt cgagcttggt tcgtatagag
    15841 ccgtaggggg tgtttagggc tttggcggca gcttgtacgc tggggaacgt gagtgtttcg
    15901 cctgtggcgg tgtcgaggat ggagacaggt ttttggtttt ggggaggcgt attttccttc
    15961 attttaagtt tcccgcactc cggacaccca tactttgact ttatagctgt gtcgtaatac
    16021 aaaagttgga caaccccgtg cgtaggacat ctaacactgg aaggtttata aatgccactg
    16081 aactccaaca attctaaatg tggaaatttc tcttgaaagc gtgatttggc ggtatcgaaa
    16141 gtcattcgct tacccataat ttgtctccat aggttatgtg caaatggatt acacaaccaa
    16201 aatatagaaa ttttcaaatt ttttgagacc tacctgaaat cctgcgcaag cacaatgtaa
    16261 agaaaaatgg tatacgtctt tactaaatct ttacataaat taactctaat cgggatttga
    16321 acgtccgtcg agcttggttc gtaggattta gggtgttgtg aaccttacct atcgtaccca
    16381 agcactttgt acctgtttga aataaggttg tcgggcgacg ttcgaccctt gagcctgtga
    16441 ttgatttgtt ggaaatgtac acctaaagca gcacctgcgg cagtaacgct tgggaacgtg
    16501 agtgtttcgt tggtcgttgt gtcgaggatg aggaggggct tccctacatt tggaggtgtt
    16561 tgggtttttg acatcttagc acactcagga cacccgtact ttgactttat aagggcatta
    16621 aatgtcgaat aacgaacgtt cccgtgcata ggacaattaa cggttactgg gtaattcaca
    16681 ccgtcaaagt cggttagaga gatattaggg aatatatcag taagttcaga agctgcttgg
    16741 ttgaaagatt tggccatttg agactccaaa attgattgtc aaatggattt aatagcaaat
    16801 ttataaaaat gtcaaatttt tcgagaagca cctgatagtg gcgaggggta tacactgtta
    16861 aaaagtgtat acgtcattta gtgcagatgt gaaccttata cctccctgca agtaaagtct
    16921 ctgtgtgaac ccgacctttt atacggtcag tgatagctgc tgggtttata cccaatgacc
    16981 ttgcagcaga tgctcttgag gggaatgtaa gtgtttcgcc agttacagta tcctccaata
    17041 ctgtattttt agataattta tctattgtat cttctggaat tttgtgggaa ttgacaccat
    17101 aagatgcaca ttttggacac ccacttttag agtccaacat acttttgaat gtagagcaag
    17161 taaccctccc gtgtatagga cagacgatta ccgtcgggta acgaacgcca ttgaactcca
    17221 caagattcaa atgtggaaat ttagaggtta gcttagaaac tgcttgagta aatgtaagtg
    17281 ccatagtggt tgtgaactta ctttaccaac ctataacgat acctaatcaa tcggtcaaac
    17341 ggagtgcgtc cgcttaaacg tacagctaaa gtgctgttgg agcaaccgat aaatttactg
    17401 gcagccagtg tacttgcaaa ctcgtagatt tcccctgtct ctgtgtcctc caacctaatg
    17461 gcaacggcgt ttggagaggg cttttcggga tttcgagtac attcaggaca tcctgttgag
    17521 gatttcatgg cagcttgtag attcccgtaa acaaccgacc catgcaaagg gcattggatt
    17581 gttaccggtt cgtagttttt agtgaacttc aaaaccgtct tatcaggaaa tttactggaa
    17641 aaccttaact gaacatcttg tagcgagagt gattttggca ttatagacct ttctaaaatg
    17701 aaagcattat acaaccaaaa tatagaaatt ttcaaatttt ctctgaacgc actggtatcc
    17761 gatggagcta aatgtaaaga aaaatggtat acgtctttac taaatcttta cacattttaa
    17821 ctgtatagtg tttgacaaac aacaggcata ggcgcataat gcacccgtag ttagatagat
    17881 agtaaggaga taaccaatga cttagcacct gcccccctct ctctcgcgca ccccgcccct
    17941 cgcttgcggc taatgggctt tgcgcgacat atacactttc atctcctgca tggcttaact
    18001 gcctatgcaa tagtacacac atcaacaaat caggctggca cgtcttggca ttggctgacg
    18061 taccattgta gcccgaaatg ttgataccct actatttatg accaacggcg gattagggct
    18121 tcagacggca attttactgc aaaaccttat gcgaacggat gatgcagcac aatcgtttct
    18181 tcgcggtcgg ggccggtgga gacgatggcg accggcgcac cgcagacttc ttcaatccgt
    18241 ttcaaatatg ctttggcatt ggcaggtaat gcgtcgtagc ttttcacgcc gaaagtggat
    18301 tcgctccaac cgggcatggt ttcgtaaatc ggcttgcagg cttctaccgc atcagagccg
    18361 caaggcagga tgtcggtttt gccgccgtcg ggcaattcgt agccgacgca gatattgatg
    18421 gtttcaacgc cgtccattac atcgagtttg gtaatacaca taccggaaat gccgttgatt
    18481 tggatggagc gtttcagggc ggcggcatca aaccagccgc agcggcgtgc gcgtccggtt
    18541 accgaaccga attcgtgtcc gcgttctgcc aaacctgcgc cgacttcgtc gaacaattcg
    18601 gtcgggaacg ggcccgaacc gacgcgcgtg gtataggctt tgacgatgcc caaaacataa
    18661 tccagcattt gagggcctac gcccgcgcct gccgaagccg cgccggcgag gcagttggac
    18721 gaagtaacga aggggtaagt gccgtagtcg atgtccaaca acgcaccttg cgcgccttca
    18781 aacagcagtt tttcgccgtt tttgtttttt tcgttcaaca cgcgggacac gtcggtaatc
    18841 atcggcgtaa tgcgcggcgc gactttttcg ataaccgcca tcacgtcttc cgctttcacc
    18901 gactcggcat tgtgcagatg ttgcagttgg acgttgtaat aagcaaggac ggcatccagt
    18961 ttttcacgca gtttttcagg atgcagcaaa tcggcggcgc gaatggcgcg gcgtgccact
    19021 ttgtcttcgt aggcagggcc gatgccgcgg ccggtcgtgc cgattttgcc tttgccgcgc
    19081 gatgcttctc gggcttggtc gagcgcgatg tgataaggca ggatcagcgg gcaggtcggc
    19141 gcgattttca gacggccttc gacgtttttc acgcctgccg cgttcaactc gtcgatttcg
    19201 cccaacaggg cttcggggga gacgacaacg cccgaaccga tgaagcagtc cagactttca
    19261 tgcaggatgc cgctcggaat caggcgcaaa atggtttttt tgccgccgac aaccaaggta
    19321 tgacccgcat tgtggccgcc ttggaagcgc accacgcctc cggcttcttc cgccagccag
    19381 tcaacgattt tacctttacc ctcgtcgccc cactgtgcgc cgattactac aacattttta
    19441 gccatagcca tataaccttc gatattaaaa attattgcgg aaatccccaa accggtgcgg
    19501 ctttgcgccc cgccacgatt tgcctttcat ccgccatgaa cgggtattta gcgtttcacg
    19561 acctgccaaa cgccgtccgt ctttttcaga cggcctgcaa gctcttccga aacattgtgt
    19621 ccgataccgt aatcgattac gacacactgc ccttgttcac gcaaggcttc gaccgcttcg
    19681 tgcgccgctt cggcatcttc cgcatcgacc aacacggcgg gctgccgttc gatggcgggc
    19741 aaacgcccga taaagctgcg caagtcgaaa ctgaatcccg ttgccgggcg cgccctgccg
    19801 aaatatccgc ccaatccgtc ataacgcccg ccgcgcgcga ccgcgtcgtg gaaatcggcg
    19861 gcataggcgg catacagcaa gcccgtgtgg taattgtcga cgcgcagctc ggacaagtcg
    19921 atatggattt cacaatcggg gaatgcgtcg cacaccgcct gcaattcgcc caacgcgccg
    19981 ccgaccgccg acaaatccgg caaccgtccg cgcgcgtcgg acaacacttc acgcccgccg
    20041 tacaggcgcg gcagcagcga gaatgctttt gcccacatac cgtccagctt ccaagccttg
    20101 acctgcgctt cgaccgtctc ggtatcttta tcctgcatca aggcaagcag cgttgcagac
    20161 tgccccgcat ccaaatgcgc cgcatcggac aaggcgcgaa atatgccgat atgccccagc
    20221 gaaagcagca ctttgcccat atcggcaatt ttcatgcttt tcagcatcag gtctatcagc
    20281 tcgatgtcgc cacggatgtc ggcaaaaccg tacatttccg cccctgcctg caagggttcg
    20341 cgcatattca gcagaccgtc ggactgcgcg tgcaacaccg gaccggcata acacaaccgg
    20401 ttaatccctt ggttggcgga taaaagatgg gcatcgatac gcgccacctg cggcgtgatg
    20461 tcggcgcgta tgcccaactg cctgccgctg agcctgtccg ttaccaaaat ggttttcagg
    20521 gaaagccccg catcgatatg cgtcagcagg gaatgtgcgt actccatcag cggaggctgt
    20581 accagttcat aaccgtgtac gcggaacagt gccaacaact gctccctcgc gctttcaagc
    20641 tgccgcgcgt tcgtgggcag tacgtcggcg atatgttccg gaagctgcca tgtctgcatg
    20701 agaatctgcc ttctatttta tcctgtaaac acaaaagact gcccgatccg caatcagacg
    20761 gcatctttgc cgcaacgcat cacgccgctg tcaaaacggc ggtgcttcct tccgattcag
    20821 aatccgaaca gcccctgtcc gacggctcaa tgccgtcaag tcgttaaaac caaactttac
    20881 cataaaatac acacaatctg aatatacttt tataccctgt atccgccgtc tgacggcacg
    20941 gcattttttt actatataat gtgcaccatt atttctcatc acttcctgct gccgttttct
    21001 gcttcagacg gcatttttgt tgattgaaca cttatgctgc tcgacctcaa ccgcttttcc
    21061 ttttccgtct tcctgaaaga agtccgcctg ctgaccgctc ttgccctgcc catgctgttg
    21121 gcgcaggtcg cgcaggtggg catcggtttt atcgatactg tgatggcggg tggtgcaggc
    21181 aaggaagact tggcggcggt ggctttgggc agcagcgcgt ttgccacggt ttatattacc
    21241 tttatgggca ttatggcggc gctgaacccg atgattgccc agctttacgg cgcgggtaaa
    21301 accgacgaag tgggcgaaac ggggcggcag gggatttggt tcgggctgtt tttgggcgtg
    21361 ttcggcatgg tcttgatgtg ggcggcgatt acgccgttcc gcaactggct gaccttgagc
    21421 gattatgtgg aaggcacaat ggcgcagtat atgctgttca ccagcttggc gatgccggcg
    21481 gcaatggtac accgcgcact gcacgcctac gcctccagcc tgaaccgccc gcgcctgatt
    21541 atgttggtca gctttgcggc gtttgtgttg aacgtgccgc tgaactatat tttcgtttac
    21601 ggcaaattcg gtatgcccgc tttgggcggc gcaggctgcg gactggcgac gatggcggtg
    21661 ttttggttca gcgcgctggc attgtggatt tatatcgcca aggaaaattt cttccgccca
    21721 ttcggactga cggcgaaatt cggcaaaccg gattgggcgg tgttcaaaca gatttggaaa
    21781 atcggcgcac ccatcgggct gtcttatttt ttggaagcca gcgcgttttc gtttatcgtg
    21841 tttttgattg cgcctttcgg cgaggattat gtggcggcgc agcaggtcgg catcagtttg
    21901 tcggggattc tctatatgat tccgcaaagc gtcggctcgg cggggacggt gcgcatcggc
    21961 ttttcgcttg ggcggcgcga attttcgcgg gcgcgttata tttcgggcgt gtcactggtg
    22021 ttaggatgga tgctcgccgt gattaccgtg ctttccttgg tattattccg ttcgccgctg
    22081 gtaagtatgt acaacaatga tccggcggtt ttaagcatcg ccgccaccgt cttactgttc
    22141 gccggcttgt tccaaccggc agacttcacc caatgtatcg cctcctacgc cttgcgcggc
    22201 tacaaagtta caaaggtgcc gatgttcatc cacgccgccg ccttttgggg ctgcggtctg
    22261 ctgccgggct acctgctcgc ctaccgtttc gatatgggca tttacggctt ctggacggca
    22321 ttgattgcct cgctcaccat cgccgccatc gccttggtgt ggtgcttgga attgtgcagt
    22381 agggagatgg tcagatcgca taaggctgtc tgaaaactca gtacacttca attcaaatac
    22441 agttaaagtt caaaccatgc aacccatccg ataccgaacc gacctcaccc cctacaacac
    22501 cttcggtctt cgcgcccaag cccgagcctt tattgcgctt gaacacgccg acgggttgcg
    22561 cgacatcgtc cggctacccg agttcaaccg agatactgtt ttatggctgg gcggcggcag
    22621 caatatcctt ttgatggagg actacgccgg tctggtcgta catatggaaa acaaaggcat
    22681 acgcgaaatt gctcgttcag acggcatggt tctgattgaa gcgcaggcgg gcgaaatctg
    22741 gcacgatttt gtcctgcacg ccgtcgcgct gggtttgaac ggtttggaaa acctgagcct
    22801 gattccgggt acggtcggcg cgtcgcccgt gcagaacatc ggcgcatacg gcgtagaggc
    22861 gaaagatgtg attcacagcg tgcgctgctt cgatttggat acggaaacct ttgtaaccct
    22921 ttccaatgcc gactgccgct tcgcctaccg cgaaagcctg ttcaagcagg aaggtaaagg
    22981 gcgttatgtg attgtttcgg tcgtatttgc attaaaaacg cattttgtgc cgaacttggg
    23041 ttacggcgat ttggcggccg ccgttgccga actgagcgcg ggcagggtgc cgacggcgaa
    23101 agatgtttcc gatgcagtgt gtgcaatccg caacagtaaa cttcctaatc ctaacgtgct
    23161 tggcaatgtc ggcagtttct ttaaaaaccc cgtcgtcagc gcggaaaaag ctgccgccct
    23221 gttggagcag catcccgata tgccgcatta tccgcagccc gacggttcgg tcaaactcgc
    23281 cgccggctgg ctgatcgacc aatgtcgtct gaaaggcttt caaatcggcg gcgcggcggt
    23341 acatggcagg caggctttgg tcttagtgaa caaaaacaac gcctcggcaa acgatgtccg
    23401 gcaattggcg caacacatca aatttacagt atttgctcgg tttcaggtag aattgcacgc
    23461 cgaacccaat tggctgcctg cttcgttcag cctgtaaatc caaggagtat gcccgtgacc
    23521 cgccccgcca aaatcaatac ttacacacgc atcatcgacg ccagccttgc gctcttcaac
    23581 gaggaaggcg agcgcaacat cagcaccaac catattgccg cccacttggg catcagtccg
    23641 ggcaatctct attaccactt ccgcaacaaa gacgaaatca tcgtccaact gttcaaacgt
    23701 tacagcgaag ccctgctggc atacctgaat gaagccgtgc tgccgtctga tgtggaagac
    23761 tctatcaact atatggcggg catttacgac gtcatgtggg aataccgctt cctctttagc
    23821 gacgtaaaca ccctgcttgc acgcagtgcc gaattgttgg gcgaacacaa caccttcacc
    23881 caagccaaag tctccccgct cttggtcaac ctgctcaccc agctcaacgg actaaacgtc
    23941 atcaacgccg accaaaccgc tatgaacgac ctcgccgtca atatgtggat ggtcacgaaa
    24001 tactggttcg acttcgacag ctccctgcgc ggtcgcacca agctgaccga agactccaaa
    24061 gcacgcggca tccgccgcac cttaagcctc cttcgtcctt atcttttgcc cgaacaccgc
    24121 gagaaatacg accggaaaat cggcaacggc aacccgtaaa cccacagcgt ccgaatcagc
    24181 cttcagacgg cacttacccg acggtatcag cgaatgcgcg cagtcccccc gccccatatc
    24241 tccatcacag gcctaggtta cctcggcctg ccgctggcac aaaagtttta ccaacacggc
    24301 agccgcgtcg ccgccataaa acgcagcctg acttcggacg acatcaacct gcccatacac
    24361 ctcgacacct tcgacctcgg cagcaccgat gcctttcaga cggcattgtg gcggcaccac
    24421 gccgacaaac ccgtctggtt cttcctgcta ccgccctcct cgctgacgca ttacgccgac
    24481 accgtcaaac aatgggcaga gcttgcccgg gcgtgcaacg tgcaacacct gattttcacg
    24541 agcagcacca gcgtttacgg cgataaggcg cgcgaatgcg acgaaaccgc cctacccgat
    24601 ccgcaaaccg agtctgtccg ccaaatcctc gccgccgaac aacacctgct cgacagcggc
    24661 atctccaaca tcgacatcct gcgacttggt gggctttatt gcgccgaacg ccatcccgtc
    24721 agccgccttg ttcaaaagca aaacatcctg ggcggcaacc gccccatcaa catcgtccac
    24781 cgtaatatcg ccgtcgaaag cctgtttcag acggcattta accccggcgg caggcggctg
    24841 aaaaacatta tcgaaccgcg ccacccgaca cgccgcgaat tttttacgga agaagccgcc
    24901 aaactcggcc tgcccgcgcc ggatttcgca cccgacgaca gcgtgggcaa aatcatccgt
    24961 accgtttgcg ataacggctt aagcctgtaa aataagcggc aacagcaaca aacaggcatt
    25021 ctccgcccca atcagaaaat atgtccgcct tcctccccat catcaaccgc ctgattctgc
    25081 aaagcccgga cagccgctcg gaacttgccg cctttgcagg caaaacactg accctgaaca
    25141 ttgccgggct gaaactggcg ggacgcatca cggaagacgg tttgctctcg gcgggaaacg
    25201 gctttgcaga caccgaaatc accttccgca acagcgcggc acagaaaatc ctccaaggcg
    25261 gcgaacccgg ggcgggcgac atcgggctcg aaggcgacct catcctcggc atcgcggtac
    25321 tgtccctgct cggcagcctg cgttcccgcg catcggacga attggcacgg attttcggca
    25381 cgcaggcaga catcggcagc cgtgccgccg acatcggaca cggcatcaaa caaatcggca
    25441 ggaacatcgc cgaacaaatc ggcggatttt cccgcgaatc cgagtccgca aacatcggca
    25501 acgaagccct tgccgactgc ctcgacgaaa taagcagact gcgcgacggc gtggaacgcc
    25561 tcaacgaacg cctcgaccgg ctcgaacgcg acatttggat agactaacct tcagacggca
    25621 tccgacatga acagcccttt tcacaacatc ggcatcgtaa cccgccccaa cacgcccgac
    25681 atccaagaca ccgcacacac gctgattacc tttttgaagc agcacggctt taccgtctat
    25741 ctcgacgaag tcggcataaa ggaaggctgc atctataccc aagacaccgt cggctgccat
    25801 atcgtcaaca agaccgaact ggggcaatac tgcgacctgg tcgccgtttt aggcggagac
    25861 ggcacctttc tctccgtcgc ccgcgaaatc gccctgcgcg ccgttccgat tatcggcatc
    25921 aaccaagggc atttgggctt cctgacccaa attccccgcg aatatatgac ggacaagcta
    25981 ttgcccgttt tagaagggaa ataccttgcc gaagagcgca tcctgattga ggccgcactc
    26041 atccgcgaag gcaaaaccgc cgaacgcgcc atcgccctca acgatgccgt cctctcccgt
    26101 ggcggcgccg gacagatgat tgagtttgaa gtcttcgtca atcgggaatt cgtctatacc
    26161 cagcgttcgg acgggctgat tgtctccacc cccaccggat cgaccgccta ttcgcttgcc
    26221 gccggcggcc ccatcatgca ggcaggattg cacgccttca cgctcgtccc catctgcccg
    26281 caatccatga ccaaccgccc catcgccatt ccagacacgt ccgaaatcga aatcctcgtt
    26341 acccaaggcg gcgacgcgcg cgtccatttc gacggtcaaa cccatatcga cgtgcaaaac
    26401 ctcgaccgca tcaccatccg ccgctaccga aaccccttac gcatcctaca tccgaccgac
    26461 taccaatatt tcaaaaccct gcgccaaaaa ctgcactggg gcgaacaatt ggtctgaacc
    26521 gacccttacc ggaaaacacc tatgaatcca aaaactcccc ccgaccatac agaagatgcc
    26581 gtgcgcctgt ccaaacgcat ggcgcaactg gggctttgtt cgcgccgcga agccgacggc
    26641 tatatcgaac agggttgggt aacggtcaac ggcaaaaccg ccgtactcgg tcagaaagtt
    26701 tcgccggcag accgtatcga actaaacaag aaagcccacg aacagcaggc ggcacgcatt
    26761 accatcctgc tgaacaaacc cgtcggctat gtcagcgcac aagcggaaaa aggctataaa
    26821 tccgccgccg aactgattac ctctgaaaac cgctgggaag gcgacaccgg ccgcatccgt
    26881 ttcgatccga aacacaaaat cggtcttgcc cccgccggca ggctcgatat cgattcagtc
    26941 ggattactgg tgttgacgca ggacggccgt atcgccaagc agcttatcgg cgaaaacagc
    27001 ggcagtgaaa aagaatattt ggtgcgcgtg cgcggcaaat tggacgaaaa aggacttgcc
    27061 ttactgaatc acggattgag tttggacggc gagaaactgc gtcccgccca agtagaatgg
    27121 caaaacgaag accaactgcg cttcgtgttg aaacagggta aaaagcggca aatccgccgt
    27181 atgtgcgaac tggtcggact gcgcgtcgtc gggctgaaac gcatccgcat gggtaaggtc
    27241 aagctcggca ggctgccgcc cggcaaatgg cgttatcttg ctcccggcga atcgttttaa
    27301 ataagcatta aaaaatgccg tctgaacgtc cttacgtttt cagacggcat tgccttatgc
    27361 ccaaataacg gtttcttcca agggcctgcg cgccttcgtg gcgatttcct gaacgcggta
    27421 accgaatgtc gcggcgacgg acacgcccca ttctgccgca tcgaacaaac cgaactgccc
    27481 ggacaatacg cgttccatat cggcatagtt gaaaccttcc accgggcagg aatcgatacc
    27541 tgccatcgcc gcacccgtca tcatgttgcc caacgcgata taggtctggc ggcaacacca
    27601 gtcaaacaag gcgcgcgaat cgtccaaaat cttgatgtcg tcggcttgaa acgcctgata
    27661 cctcgccaaa gattttgcca tggcatccgg ttcggtaacg ccgcgccgtt tgaggctttc
    27721 caacataaac gggctgtcga agcgggcatt tttctttgcc aaaaacacca ccaaatgact
    27781 ggcggtatcc aaagcatccg ccatacccca agaaaacggc ttgattgcct gtcggatttc
    27841 ggggttttga accacaacaa actgccaagg ctccgaaccg accgaactgg gcgacaaacg
    27901 cccgagttct aaaataaact gaaaatcctc ggcactgatt ttacgtgccg catcgtaatg
    27961 ccggcatgat ttgcggtttt taaatgcgga tagaacctgc tctctgtcta ataccgtcat
    28021 agtgcatcct ttcattactt gtattgcaaa ttgatttcaa accgatacgg cggcagattc
    28081 gccaataacg cccaaacgcc tttcagacgg catttggttc atcgcaaaca cgcctacggg
    28141 taggcgatat aggcataagc cgaatggttg tggatagact cgaaattctc gctctcgacg
    28201 acgaaactct tgatgcgttt gtcggcgatc agcgaagtag cgacatcgcg caccatatct
    28261 tccacgaatt tcgggttttc gtaggctttt tcggtaacgt atttttcatc ggggcgtttg
    28321 agcaggccgt agagttggca gcttgcctgt gcctccacat aatcgatgac ttcctcaata
    28381 ccgacttcgg catcggcagt caggctgacg gtaacgtgcg aacgctggtt gtgcgcgcca
    28441 tattgggaaa tttctttgga acacgggcaa agcgaggtta cgggaatcat gaccttcata
    28501 ctgtggccgt atgccccgtc tttgatttcg cccgtgagac agacatcata atccagtaag
    28561 gaccgaatac cggaaaccgg cgcggttttc ttgcggaaaa acggaaaaga aacgctgatt
    28621 ttgccggcgc gggaatctaa aagcgcgacc atttcggtag tcagcttgcg caattgtgca
    28681 aaatccaagg cttcggcatg ttgctccatc aatgcgacaa aacgcgacat atgcgtcccc
    28741 ttctgctcag caggcaggta aaccgtcatg gtcagacggg caatggtgga ctggatgcct
    28801 tctgcagttt gcagggtaat cggaaagcgc aggtctttaa tgccaacctg attgatcggc
    28861 agattgcgca aatctcggct ggattgcacg tctgcaatag tgttcatgag ttgtttttcc
    28921 ttaatattca tgtaattgtt gagatgtgtg catggtatgg cgtttccgcc ggggaatata
    28981 ccgtcaatct gcaaatgcga gattatatca ctcgctttta gcagctgcat tgattgtttc
    29041 tatttttcag atagtgataa aatcttcgat tattcacatc tacataggca gagaccaaat
    29101 gaaattcgcc acgaaagcca ttcattccag ctacgattgc gacgaacaca accgcgcgct
    29161 gatgccgccg atttatcaaa acagtatgtt tgcgttgcac gagattggcg aaaatgtgcc
    29221 ttaccgttat tcgcgcctga gcaacccgac ccgtcagatt ttagaagaca ccgttgccga
    29281 tttggagcat ggtgcagccg gttttgcgtt ttccagcggt atggcgggga ttgatgccgt
    29341 gtggcgtact ttcctgcgcc cgggcgatac cattgtcgcc gtcgccgata tttacggcgg
    29401 cgcttatgac ttattggtcg atgtttatca aaaatggggg gtgaacgttg tttttgccga
    29461 tttgggcaat cccgataatc tggacgagct gctcaaagcg cacaaggtca aactggtctg
    29521 gctggaaacg ccgtccaatc cgcttttacg cttggtagac atcaaagccc ttgccgcgaa
    29581 agccaaagcg accggcgcac tggtcggtat cgacaacacc tttgccacgc cgtatctgca
    29641 acagcccttg gatatgggtt gcgattttgt attccattcc gctaccaaat atttgtgcgg
    29701 ccattccgac gtattgatgg gcatcgtcgt tgccaaaacc aaagaactgg cgcagccttt
    29761 gcacgatatg atggtgcata ccggcgcggt tgccggcccg ctggactgct ggctggtgtt
    29821 gcgcggcatc aaaacactgg ctctgcgcat gaacgcccat tgccaaaacg cactcgaaat
    29881 cgcgcgccgt ttggaagccc atcctgccat tgaaaaagtg ttctatcccg gcctgccgtc
    29941 gcacgaacat tacgaactcg cccaagcaca aatgcccaaa ggcatcggcg gcgtggttac
    30001 ggtttatctc aaaaacgaca cgcgtgaagc ggcaaacagc gtgattaaaa acatgaaact
    30061 ggtcaaaatg gcttccagcc tcggcggtgt ggaaagtttg gtcaaccatt gctattccca
    30121 gtcccacagc ggcgtgccgc ataatgtgaa aatggaaatg ggcatcaaag tcggtctgct
    30181 gcgcttctcc atcggcatcg aagacgcgga cgatatttgg aacgatattt ccgccgcact
    30241 cgatacaact ttgtaaaccg taaaaatgcc gtctgaaacc atggtttcag acggcatttc
    30301 aattaacccg gccgaaaatc aacgcttcag catcttggcc gcctcaatgg cataataggt
    30361 caaaatccca tccgcgcccg cacgtttgaa tgccagcagg ctttccaaaa ccactttatc
    30421 gccatccagc catccgttgg caatcgctgc ctgcaacatc gcgtattctc ccgaaacctg
    30481 ataggcataa gtcggcacac cgaactcgtc ctttacgcgg cggacaacgt ccaaatacgg
    30541 caaaccgggc ttgaccatta ccatatccgc accttcctga atgtccaacg ccacttcgtg
    30601 caacgcctca tcggtatttg ccggatccat ctggtaggtc tttttatctg ccttgcccaa
    30661 attgcccgaa ctgcctaccg catcacggaa agggccgtaa aatgcagaag catatttggc
    30721 ggaatacgcc ataatccgcg tatggatatg cccggcatcc tccaacgcct cgcgaatcgc
    30781 accgatacgc ccgtccatca tatcggaagg ggcaaccacc tgcgcgcccg cttcagcgtg
    30841 gcacaaagcc tgcttgacca aaacctctac ggtttcatcg ttcatcacat aaccgttttc
    30901 gtccgtcagc ccgtcctgac cgtgaaccgt ataaggatcg agcgcgacat ccgtcataat
    30961 gcccagttcg ggaaacctct cgcgcaaggc gcggacagtt gacggcacga gtccttcggg
    31021 attgtacgcc tcctgcgcac gctcggtttt gtttgccgta accacgggga acagtgccaa
    31081 catcggaata ccgagcttta ccgcctcttc cgccgtaaac agcagcctgt ccaaactttg
    31141 acgcttcaca cccggcatag aaggcacatc ctcctcgcgc gccgacccct ccaatacgaa
    31201 caccggataa atcaaatcat cggcggtcag cgtgtgttcg cgcatcaggc ggcgtgaaaa
    31261 atcgtccctg cgcatacggc gcatacgcga agccggaaca ttgcggtaag gaaactgcat
    31321 aagccctcca atcatcctgt caacaattca aaccatacgg aagccgccgc aacttgcaga
    31381 cagcatcggg cggcaatcaa aatccgtttt caccggaaag ggtttcggcc cgccgaaatg
    31441 ccgcgccgaa ggacggcgtc ccgcccatta aagaacaata gcaaaacaac agcttcaggc
    31501 tgtcagcctt cgatattctt acgccacaat accaaaagtt tcccgatatg ctgaacaggt
    31561 tgcgcgtcaa ccgcctcaca taaggcagtg cagatttcga tacgttcggc acggtcgtcg
    31621 ccgaatacgc gcactttaat cagctcatgc gccgtcaatg ccgcatcggt ttccttgatg
    31681 accgcgtccg tcagaccctg ctgaccgacc atcacaacag gatggagatg gtgcgcgcgc
    31741 gctttcagtt ccaaaatttc tttggtgttc aatttggtat ccgtcatttt cctactgctt
    31801 gaataaaaag aataatgcgg cattgtacgc gatttggcac gacatcggca aaattaacgt
    31861 acaatacgcg gtttccattc cgacccgcga accactatgg ctgtacgttc caaatcctca
    31921 aaagcgtggc tgcacgaaca cgtcaacgac cactacgtcc atatggcgca aaaagacggc
    31981 taccgcgccc gtgccgcata caaacttttg gaaatcaacg aaaaagacaa attaatcaaa
    32041 cccggcacgg tacttgccga cttgggcagc gcaccgggaa gctggtcgca ggttgccgcc
    32101 aagctgacgg gtacttccgg agcagttttc gccttggaca tcctgcctat ggaagccata
    32161 gggggcgtct ccttcattca gggcgacttc cgcgagaacg acgtactggc acaattcgaa
    32221 accttgttgg acaaccgccc gctcgacctt gtaatttgcg atatggcgcc caatatgtcg
    32281 ggaaacgccg taagtgatca ggcacgcagc ttttatctgt gcgaactggc tttagacttc
    32341 gcctcgcaac acctgaaaac cggcggcagc tttttggtca aagtctttca gggtgcaggc
    32401 tatcaggaat acatggcagc catgcgcgaa attttcggca cggtgcagac gcgcaaaccc
    32461 gaagcctcgc gcaatcgctc cagtgagatt tatttattgg gcaaaaataa acgctgacaa
    32521 tacagacggc gtgctttaca atcatttccg ttttacacct caattatgga gccttgctaa
    32581 gtggggaaca cctttaaatc aatccttgtc tgggtcgcct tgggtatcgg cctgatggct
    32641 gcgttcaacg ctttagacgg taaaaaagaa gacaacgggc aaatcgaata ttctcagttc
    32701 atccaacagg tcaacaacgg cgaagtatcc ggcgtcaaca tcgaaggatc cgtcgtcagc
    32761 ggctacctga ttaagggcga gcgcaccgac aaaagcacct tcttcaccaa cgcgcctttg
    32821 gacgacaacc tgattaaaac actgctcgac aaaaacgtcc gtgtaaaagt aacgccggaa
    32881 gaaaaaccga gcgcgctggc tgccctgttt tacagcctgc tgcccgtcct gctgctgatt
    32941 ggcgcgtggt tctactttat gcgtatgcag acgggcggcg gcggaaaagg cggcgcattc
    33001 tcattcggca aaagccgcgc ccgcctactg gacaaagatg ccaacaaagt tacctttgcc
    33061 gatgtcgccg gctgcgacga agccaaagaa gaagtgcagg aaatcgtcga ttacctcaaa
    33121 gcgccgaacc gctatcaaag cctgggcggg cgcgtgccgc gcggcatcct gctggcgggc
    33181 agcccgggta cgggtaagac gcttttggcg aaagcgattg caggcgaagc cggcgtgccg
    33241 ttcttcagca tttcaggttc cgactttgtc gaaatgttcg tcggtgtcgg tgcaagccgc
    33301 gtccgcgata tgttcgagca ggcgaagaaa aacgccccct gcatcatctt tatcgacgag
    33361 attgacgcag tcggccgcca acgcggcgca ggtttgggcg gcggcaatga tgagcgcgag
    33421 caaacattaa accaattgtt ggttgaaatg gacggttttg agagcaatca gactgtaatt
    33481 gtgattgcgg caaccaaccg ccccgacgta ctcgatcctg cgctgcaacg ccccggccgt
    33541 ttcgaccgcc aagtggttgt ccccctgccg gacatccggg gacgcgaaca gattttgaac
    33601 gtccattcta aaaaagtgcc tttggacgaa tctgtggatt tattgtccct cgcgcgcggc
    33661 acgccgggtt tttccggcgc ggatttggcg aacttggtca acgaagccgc cctgtttgcc
    33721 ggccgccgca acaaagtcaa agtcgatcag agcgattttg aagacgccaa agacaaaatc
    33781 tatatgggtc cggaacgccg cagtatggtg atgcacgaag acgaaaaacg tgcgacggcg
    33841 tatcacgaat ccggacacgc gattgttgcc gaaagcctgc cctttaccga ccccgtccac
    33901 aaagtaacca ttatgccgcg cggacgtgcg ctgggtctga cttggcagct tccggagcgc
    33961 gaccgcatca gtatgtataa agatcagatg ttgagccagc tctccatcct gttcggcgga
    34021 cggattgccg aagacatctt cgtcggacgc atctccaccg gcgcatcaaa cgactttgaa
    34081 cgcgcaaccc aaatggcgcg cgaaatggta acgcgctacg gcatgagcga caaaatgggc
    34141 gtgatggttt atgcggaaaa cgaaggcgaa gtcttcttgg gacgcagcgt aacccgttcg
    34201 caaaacattt ccgagaaaac ccagcaggac atcgatgcgg aaatccgccg gattttggac
    34261 gagcagtatc aggttgccta caaaatcctc gatgaaaacc gcgacaagat ggaaacgatg
    34321 tgcaaagccc tgatggaatg ggaaaccatc gaccgcgatc aggtactgga aatcatggcg
    34381 ggcaaacaac ccagcccgcc caaagattac agccacaacc tgcgcgagaa tgcggacgcg
    34441 gcggaagata acgcgccgca cgctccgact cgggaagaaa ccgaagcacc tgccccggca
    34501 gacaccgctt cgacagagtc cgagcagcag cctgaaaaca aggcttaact tcccgaacaa
    34561 acggcagccc gcaagctgcc gttttcccat cctgttttca gacggcattt tccgctcaat
    34621 gccgtctgaa acgccaaagc cgtatcggtt tttgcgaatc tgcgatatgg cggtttaaat
    34681 tgaaatcaga acggggcaag gctgtaccgg tttaaaatta aaccgctata ataccgccct
    34741 tcgaatacac gcccgcattg gtgatgcccg tgaaaaaagt tgaaaaaaac atcttggtgc
    34801 tgcacggcgc ggacaaaatg ttcgagctgg tcgataaggt tgaggattac ccgaactttc
    34861 tgccgtggta cagcaaaacc gaggtcatcg gacgcaacgg caacgaactg aaggcgcggc
    34921 tgtttatgga ctatatgcac gttcgccaat cattcgccac gcacaaccgc aacattccgg
    34981 gcagggaaat ccgtatggaa ctgctcgaag gtccgttcaa aaccttacgt ggaacgtgga
    35041 aatttatcga tttgggcgac gatatgtgca aaatcgaatt caatttggaa tacgattttt
    35101 ccaatgccgt tttgtccgcc ttaatttccc ccgtcttcaa ccacctttcc gccacgctgg
    35161 tcgaagcgtt cgtcaaagag gcagaccgcc gttatgcttg aaattgagat tgtgtacggg
    35221 ctgcccgacc gacaggtttt gaaaaccatg cagcttgccg agggaacaac cgtccgcgcc
    35281 gccgcactgc aaagcggttt ggacggcata tttgaagatt taaacctgca ttccgcgcct
    35341 ttgggcattt tcggcaaagc cgtcaaagac gacacgccgc tgcgcgacgg cgaccgcatc
    35401 gaagtgtacc gcccgctgtt gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat
    35461 caagaagaat aaccatgccg tctgaagcct tcagacggca ttcggcagaa acacggaaaa
    35521 tatcatgtca aacacaatca aaatggttgt cggcttgggc aacccgggca aagaatacga
    35581 acagacacgc cacaatgcgg gtttttggtt cctcgacgaa ctggcgtgga aatggaaggc
    35641 ttcatttaaa gaagaaaaaa aattcttcgg cgaagtcgcc cgtgctaccc tgcccgacgg
    35701 cgatgtctgg ctgctcaagc cgaccacgtt catgaaccgt tccggacagg cggttgccgc
    35761 ccttgcgcag ttctacaaaa tcaaacccga agaaatcctc gtcgtccacg acgaactcga
    35821 cattccctgc ggacggatta aattcaaact cggcggcggc aacggcggac acaacggctt
    35881 gaaagacatt caggcaaaac tcggcacggc agactattac cgcctgcgcc tcggtatcga
    35941 ccacccaggc gaccgcaacc tcgtcgtcgg ctatgtcctg aacaaaccca gtacggaaca
    36001 ccgccgacag attgacgatg ccgtcgccaa atccctgcaa gccatacccg acatccttgc
    36061 cggcaaatgg gaagaagcaa cccgcttcct gcacagcaaa tgacccgatg ccgtctgaag
    36121 ccctttcaga cggcatgttc ccgatttcca tatccgaaca gtcatgaccg aactcaagca
    36181 gcttatccaa accgaatcca tccccgtcat cgaagaaacc ctcgatttcc tgctgtacga
    36241 atgcagcatc gacgacgcac catccgccga agaagtggca caatggcgcg acatacttgc
    36301 cgcacgcggc ggcaaattcc tgcgcctgtc caaaatctgc caaacgtggc tggacgagga
    36361 ggcggcatga agctgccgcg caaccgcttc agcctgcttt ccgcattgtg gtttgccggc
    36421 ggcatctatt cgctgctctt caaagctgcc gacaccgcgc cgccgccgtt tccgcatttc
    36481 gacaaagcag cacaccttgc cctgtttttc gcacaaatct ggcttttgac caaagcattc
    36541 aaaaccggaa aacttcccat cccctaccgc agcctgatgg tctttgccct ctgtttcgcc
    36601 ctcttcagcg aatgcgcgca ggcatggttt accgcaacga gaaccggcag tttgggcgat
    36661 gttcttgccg atatggcagg tacggttctc gcactctttg ccgcccgcgc cgccgaccgc
    36721 ccggactgaa tcggtttatt tcccccaaac aggatgcacc tcttcccgat acatcctatt
    36781 tttcccctta catatcaatc ccatctcata aacaaaaccg acaaatcgtt tacaatatat
    36841 ttacactaca tcagattaca aatatactcg aaccagttgc agaatcggcg gttccacaaa
    36901 ccgtttcgga ttgcacgaca caaaagaaaa agcgatttta ttgccttaaa ggagtattca
    36961 tgaacctgca tgcaaaggac aaaacccagc atcccgaaaa cgtcgagctg ctcagtgcgc
    37021 agaagccgat taccgacttt aagggcctgc tgaccaccat tatttccgcc gtcgtctgtt
    37081 tcggcattta ccacatcctg ccttacagcc ccgatgccaa taaaggtatc gcgctgctga
    37141 ttttcgttgc cgcactttgg tttaccgaag ccgtccacat taccgtaacc gcactgatgg
    37201 tgccgattct cgccgtcgta ctcggtttcc ccgacatgga catcaaaaag gcgatggctg
    37261 atttttccaa cccgattatc tacatttttt tcggaggctt cgcgcttgcc accgccctgc
    37321 atatgcagcg gctggaccgt aaaatcgccg tcagcctgtt gcgcctgtcg cgcggcaata
    37381 tgaaagtggc ggttttgatg ttgttcctcg ttaccgcctt tctgtccatg tggatcagca
    37441 acaccgccac cgccgcgatg atgctgcctc tagcaatggg tatgctgagc cacctcgacc
    37501 aggaaaaaga acacaaaacc tacgtcttcc tcctgctcgg catcgcctat tgcgccagca
    37561 tcggcggctt gggcacgctc gtcggctcgc cgcccaacct gattgccgcc aaagccctaa
    37621 atctggactt cgtcggctgg atgaagctcg gcctgccgat gatgctgttg attctgccct
    37681 tgatgctgct ctccctgtac gtcatcctca aacctaattt gaacgaacgc gtggaaatca
    37741 aagccgaatc catcccttgg acgctgcacc gcgtgatcgc gctgttgatt ttccttgcca
    37801 cagccgccgc gtggatattc agctccaaaa tcaaaatcgc cttcggcatt tccaatcccg
    37861 acaccgttat cgccctgagt gccgccgtcg ccgtcgtcgt cttcggcgtg gcgcaatgga
    37921 aggaagtcgc ccgcaatacc gactggggcg tgttgatgct cttcggcggc ggcatcagcc
    37981 tgagcacgct gttgaaaaca tccggcgcgt ccgaagcctt gggacagcag gttgccgcca
    38041 ccttttccgg cgcgcccgca tttttggtga tactcatcgt cgccgccttc attatttttc
    38101 tgaccgagtt caccagcaac accgcctccg ccgcattgct tgtaccgatt ttctccggca
    38161 tcgctatgca gatggggctg cccgaacaag tcttggtatt cgtcatcggc atcggcgcat
    38221 cttgtgcctt catgctgccg gttgccacac cgcctaacgc gattgtgttc ggcacgggct
    38281 taatcaagca acgcgaaatg atgaatgtcg gcatactgct gaacatcctc tgcgtagtat
    38341 tggttgctct gtgggcttat gctgtactga tgtaaaccat cgacctaaac aacaagaccg
    38401 tctgaaagaa tatttttcag acgaccttga agtttgtcgt tcagacacaa tttgtcgaat
    38461 cattcaaaac cggattctaa cgaaaggaaa cccatgatta tcctgcacac caacaaaggc
    38521 gacatcaaaa tcgaactcga tttcgacaaa gcccccgtta ctgctaaaaa cttcgagcaa
    38581 tacgttaaag acggcttcta cgacggcgta atcttccacc gcgttatcaa aggcttcatg
    38641 attcaaggcg gcggcatgga tgaaaacatg aacgaaaaag aaacccgcga tccgattcaa
    38701 aacgaagcgt ccaacggcct gcccaacgat aaatacacca tcgccatggc acgcacttcc
    38761 gacccccatt ccgccagcgc gcaattcttc atcaacactg ccgacaacgc tttcctgaac
    38821 ttccgttcta aagagctgta cggcaaaacc gtcgtccaag actggggcta tgcagtattc
    38881 ggtaaagtcg ttgacggttt tgacgttgtc gatgccatcg aaggcgtatc caccaaacac
    38941 cacggctacc atgacgacgt accgagcgaa cctgtcgtca tcattaaagc cgaagcggta
    39001 taaaccggca atccgaaagc agcctgcaca aaggctgctt tttttgtttc aggcggcata
    39061 aaatccaagc acatatgccg tctgaacacc tcccgcaagc cttcggacaa tgttgcaacg
    39121 tgttaaaata gcctcctttc ctgaaaccac aacaaggaaa ccatcatggc aaatattgcc
    39181 cgcgacatct tcaaagccta cgacatccga ggcattgtcg gcaaaaccct gactgacgat
    39241 gccgcctatc tcatcggcag ggccatcgcc gccaaagccg ccgaaaaagg catgacccgc
    39301 atcgcgctcg gacgcgacgg acgcttgagc ggtcccgaac tgatggagca catccaacgc
    39361 ggctttaccg acagcggtat cggcgtcctc aacgtcggta tggtcgccac gcccatgctc
    39421 tattttgccg ctatcaacga atgcggcggc agcggcgtga tgattaccgg cagccacaat
    39481 ccgcctgatt acaacggttt caaaatgatg ctcggcggcg acacgcttgc cggcgaagcc
    39541 atccaagagc ttttgtccat cattgaaaaa gacggttttg ctgccgccgg caaacaaggc
    39601 agcgtaaccg aaaaagacat ctccggcgaa taccacaacc acatcgtcgg acacgtcaaa
    39661 ctcaaacgcc cgatgaacat cgccattgac gcgggcaacg gcgtgggcgg cgcatttgca
    39721 ggcaaactct accgcgcttt gaggtgcggc gttaccgaac ttttctgcga cgtggacggc
    39781 actttcccca accaccatcc cgacccatcc aaaccgaaaa acctgcaaga tttgattgcc
    39841 gcgctgaaaa acggtgatgc cgaaatcggc ttggcgtttg acggcgatgc cgaccgcttg
    39901 ggtgtggtca ccaaagacgg caacattatt tatcccgacc gccaactgat gctgttcgcg
    39961 caagacgtgt tgagccgcaa tcctaaagcc aaagtaattt tcgacgtcaa atccacccgt
    40021 ctgcttgcgc cttggattaa agaacacggc ggcgaacctg tgatggaaaa aaccggccac
    40081 agctttatca aatcctccat gaaaaaaacc ggcgcgccgg ttgccggcga aatgagcgga
    40141 cacatcttct tcaaagaacg ctggttcggc ttcgacgacg gtctgtacgc cggcgcacgc
    40201 ctcttggaaa tcctgtctgc ctccgataat ccgtccgaag tgttaaacaa cctgccgcaa
    40261 agcatttcca cgcccgaact caacatcgcc ctgcccgaag gcagcaacgg ccatcaggtt
    40321 atcgacgaac tcgccgccaa agccgaattt gaaggcgcaa ccgaaatcat caccatcgac
    40381 ggtctgcgcg ttgaatttcc cgacggcttc ggtctgatgc gtgcttccaa taccacgccg
    40441 attctggtgt tgcgttttga ggcggatacg caagcagcca tcgagcggat tcaaaaccaa
    40501 ttcaaagccg tcatcgaaag caatccgaat ctaatctggc ctctgtaaac acaggaaaaa
    40561 tgcttaatca aatcttggta gattggattt ggcttaaaaa tctccgcgtc cgttcgtgtt
    40621 tggggtggtc gaacaactct ttcgggctgc cctgctctac gataacgccg ccgtccatca
    40681 cgacaacggt cgtggcaact tccagcgcga acttgatttc gtgggtaacg acgaccatcg
    40741 tccaaccttc ccgcgccaat tccttcatgg cgttcaacac gtcttgcacc aactcggggt
    40801 caagcgcgga agtgggttcg tcaaacaaca tcagctcggg ctgaatcgcc aatgctcggg
    40861 caatgccgac gcgctgctgc tgaccgccgg aaagctggta gggatacaaa tccactttgt
    40921 cgcccaagcc gactttttcc agcagtttca gagcctcttc gcgcgcttgg gcggcaggct
    40981 tgccctgtac ggcaaccggt ccttccatca cgttttccaa ggcggttttg tgcggaaaga
    41041 ggttgtattg ttgaaacacc atgcctgatt tgcggcgcag tgccaaaata tcgtgtttgc
    41101 ttggtttttt agaaaaatcg attttcagcg gtcgctcgtt gtcgaactcg atttgtccgt
    41161 cttcgggcat ttccaacgcg tttaggcatc gcagaaacgt cgttttgcct gagccggaag
    41221 gcccgaggat gacgaccacc tgccctttgc acacatccaa attgatgccg cgcaaaatgg
    41281 tatttttgcc gaaggtctta tggatattgc ggattttaat catgacaact ccttatttgg
    41341 cgacgtagcg gtcgaaacgt ttttccaaac gcgcctgaat caggaacagc actttacaaa
    41401 aacaccagta aaccaaagcg gcttcgatat agacgggcaa aaagtcataa gtgcggtttg
    41461 ccgtttcctg cgcgacgcgg aataattccg ttaccgtcac gactgccgcg agcgaggtgt
    41521 ttttaaacaa accgataaac tcgttgctca aaggcggcac ggcaacgcga aatgcctgcg
    41581 gcgcgacgat gcggcggaac gtctgcatat aggtcatgcc gatggagaaa cctgcttccc
    41641 attggccttt cggtacggac aaaatcgccg cgcgtatggt ttcggaagcg tatgcgccga
    41701 cattgagcga aaagccgatg atggcggcag gaatcggatc gatatagatg ccgacggatg
    41761 gcagtccgta aaacacaatc acaagctgaa ccaacagcgg cgtaccgcga atgacggaaa
    41821 tataaaattc caccaatttc agcaggattt tccgcacgat gccgccggcg ggcataatcc
    41881 gcaccaaagc cacggctacc gcaatcatca taccgataac gaaagaagcg atcgctaaag
    41941 gcaaagatac tgcgaagccg gctttgacca taggcaaaaa cgcgctgaca atcatatcgg
    42001 cgcgtgtttc cgtcataaac ggcagcgaag caaggaaatt attgaacact gatgtctttt
    42061 ccgaagaatt gttcgcccag ttttttcaac gtgccgtcgg ctttcagctc gttgattgcc
    42121 gtactgaatt tcgccacggc ttcgtcattg cccttgttga caatcaggcc ggaaccgact
    42181 ttttcatcgg caggtgcgga ccaaacgatt ttcacgcccg cattcgggtt tttcttcaga
    42241 tagtccaaaa ccgccaattc gtcgttcagg gttgcatcgg cacgtttttg ttcaatcagg
    42301 gtcagcgatt gcgccaaacc gtcaacagcc accaaatctg cgcctgcagc tttggctttt
    42361 tcgccgtagt tgctggtcag ggattgtgcg gttttcacgc ctttgatgtc gtcgatagat
    42421 ttgatgttgc tgtcgttacg ggcaaccaat acggcaccgc tccagctgta aggatcggat
    42481 ttgtcgaatg tcgcttggcg ttcggggctg gtcagaccga cttggtttgc caccacgtcg
    42541 aaacgccccg ccttcaaacc cgccatcatc gaatcccatt gcgtttcttt aaactcgact
    42601 ttcacgccca gtttttccgc cacggcgcgg gttacttcca catcgtaacc ggtcagtttg
    42661 ccgtccttgt cgtggtaggt aaacggtgcg taagtgcctt ccgtgccgac ggtaaccgtg
    42721 cctttattgt tgatgcgctc gattaaagaa ccggaaactg ccgattgtgc aggcgcggaa
    42781 gatgctccgc tgccgccttc cgaaccgccg caggccgcca aaaccaatgc ggcaataccg
    42841 ccgagtacga tttttttcaa catcattctc tcctgtaaaa agtgtgcaag tgcggcattc
    42901 taagtgaaat tgcggcaaac acaaagaatc ctttttttat atggttattc caaatcggaa
    42961 tggaaaagat atgccgaagc cgccggaagc ggaaataaag ggggcgagaa aggcggcggg
    43021 cggcacgaaa ccgaaaagcg gcatcaggta tgcaaacggc ggattcacgc acgccgttcc
    43081 ggtaaaaatc cgcctgccga ttcggtgcta taatccatcc gaaacagatg acaccaaaaa
    43141 ggaacatctc atgaacacgc cccaatccgc catcattccc gaccacgccc aagccggcat
    43201 ctttatcgaa gccgacttcg ccgccaaccg cctcaacgat attaaagccg cctgccgcgc
    43261 ttcgctcgac gcgttaactg ccttgaaagc ccgctttccg gacgatattt tgggtctgac
    43321 catcgccttc ggcagcaaag cctgggcaac attcggacat acggacgaag gcagtgaaat
    43381 caaacccttc tccgaaatgg gcaacgggct tgcgccgtcc acgcagcacg atatgtccat
    43441 ccacatccaa tccttccgcc aaaacgccgc ctacgcgctt gcccaatccg ttttgggcgc
    43501 gttcggcgac agcatatgcg tcgcgtccga agaacacggt ttgcgcctgt atcaggatcg
    43561 cgggctggac ggtttcgtcg acggcacgga aaacccgcag ggcgatgaaa atgtccgcga
    43621 agtcgccatc atccccgaag gaaaacccga tgcgggcggc agctatgtcc tgctgcaaaa
    43681 atacctgcac gatttgaaaa aatgggatgc cgtccccgtc gccgaacagg aggcctcggt
    43741 cggacgcggc aaggaaaccg acgacgaatt cggccacgat gtccgcctgc ccgattcgca
    43801 cctcggccgc gtcaacctga aagaaaacgg agtcggcctg aaaatcgtcc gccgcagcct
    43861 gcccttcggc aaaatcagcg gcgaacacgg tttgatgttt accgcatact gccgcacgct
    43921 gcacaatatc gaagcgcaat tattgagtat gttcggcgac acggatggca aaaccgacct
    43981 gctgctccgg cacctctccg ccgccgtttc gggcggatac tactacgcgc cgtctgtcga
    44041 acgcctgcaa aacctctaag ccagacgatg ccgtctgaaa ccctttttca gacggcattt
    44101 tcccgtttta ccgtccaacc gttttccgag cgcgcactat gtctgcaccc atccaagcat
    44161 ttgacccgct gaccgtcccc atttccggca ccaacctgat tgaggcctct gccggcaccg
    44221 gcaaaaccta cggcatcgcc gccctgttta cgcgcctgat cgtattggaa caaaaaaacg
    44281 tcgagcgcgt attggtcgtt accttcacca aagccgccac cgccgagttg aaaacacgcc
    44341 tgcgcgcacg tttggacgat gtgttgcagg ttttagaaag taaagaaatt gccaaacttg
    44401 gagacgacac gctttcagac ggcatcgccg cctactgcgc cgaacaccac aaaggcgaca
    44461 ccttcctgcc cgcactctta aaacaggctt tgcaaaaaga gggccggacg cgtctgattg
    44521 tccgcctcaa ggccgccatc gggcaattcg acaacgccgc catctatacc atccacggct
    44581 tctgccaacg tatcctgcgc gactacgcct tcttgtgcca agcaccgttc gatgtcgaac
    44641 tgaccgaaga agacggcgac cgcctgctta tcccggcgca agatttttgg cgggaacgcg
    44701 tcagcaatga tccggtgctt gccgcattgg cgtttaaacg caaagctgtg ccgcaaaccg
    44761 tccttgccca aatttcgcgc tatctttccc gcccgtacct gaatttccgc cgtccgcagg
    44821 cggatttgaa acaggcgcag cgcaacgccg aaacctcatg gcaaaccatc tgccgcctgc
    44881 tgcccgagct ggaagccggc ttttggcgca tccatcccga cctcaacggc aacagttacc
    44941 gcaaaaacag cttcggcaac ctttttaaag aactggcaca aaaatccgcc gccggacaac
    45001 tgccctttct ggacaaagac acgcacgaca gactcttgaa acttgcgtcc gacaaactcg
    45061 aagccgccct taaaaaaggc aaaacgcccg atgcggcagc atttgccgaa ttgcagaaac
    45121 tggcagactt cgggcgcgat ttgaacgcac tcgaagaagc ggaagaagca acaatgatcc
    45181 ggctgcaact ggatttaatc ggatacctca accgcagcct tgccgagatg aaaaaatcgc
    45241 gccgcgaacg cggtttcgac gacctgctgc tcgatgtcca caccgcgctg accgacaatc
    45301 cgcacgccga aaccctcgcc cgcgccgttg ccgaaaactg ggaaaccgcg ctgatcgacg
    45361 agttccaaga caccgacccg ctgcaatatg aaatcttcca aaagattttc attgcccaaa
    45421 accgcccact gtttctcgtc ggcgatccca aacaggcgat ttacagcttt cgcggagcgg
    45481 acatttacgc ctacctgcaa gccgccggag acgcgcggca ccgctacacg ctcgccacca
    45541 actaccgcag ccacgccgcg cttatcggca gcataggcgc gctgttccgc ctcaaagaac
    45601 gcccgttcgt tttggaaaac atcggctatt cggaagtcgg tgcggcgcgt gccgaaagca
    45661 ggttgtcccc cgaacgtcct gccgtacagg ttcgttggct gcacgaaaac gacaatgaaa
    45721 aagccaacaa agacgttttg cgccgccgtg ccgccgacta ttgcgccgac gaaatcgccc
    45781 acgcgctcaa cgaagccgcc ggaggccgtc tgaatttcaa aggctgcccg ttgcagtcgg
    45841 gcgatattgc cgtgctggta cgcacgcaca acgaggcggt gatggtttcc gccgccctga
    45901 aaaaacggca ggtgcaaagc gtcctgcttt cgcgcgaatc tgtgttcgcc tcgcccgaag
    45961 ccgccgccct gtccgcactc atcggcttct ggctcgaacc gcgccgcgcc ggaacgctgc
    46021 gctttgtcct gacaagcggc atattcggtt atgacgcgca gcaattgcac gacttcaacc
    46081 aaaacgaaag cgagattttg cattgggcgg aatctgcccg aaccgcactc gacaactgga
    46141 atcaatacgg cattttcgcc gctatgcagc aattttccca aacacacggc atcgaaacgc
    46201 gcctcttaag ccggaacaac gggcgcagcc tgaccaacta tttccaactg ctcgaactgc
    46261 ttgccgccga agacgcgcaa aaccgcaacc cagccgcgct gcacaaatgg ttgcgcgacc
    46321 aaatcagcct ggccggcaac aacagcggcg acaaccgcgc catccgtctg gaaagcgacg
    46381 aagatttggt caaaatcgtt accatgcacg cctcgaaagg tttgcagtat ccgctggtgt
    46441 actgcccgtt tgcgtgggac gcgcaagata ccgggccgtc cgactggcaa atcctccacc
    46501 aaagcgcaaa ccgaaccgaa ctgttggcaa aggcgcaact gtcggaagac gaacagaaac
    46561 aatacgccga tgaagaaatg gcggaacgcc tgcgcctgct ttatgtcgca ctgacgcgtg
    46621 ccgaggaaca gctcaacatc tacgccgcgt actccaccaa tactgccgac aaccccctcg
    46681 cctacctgat tgaaggctcg ccccaagaca gccgtgaaac cgtccgccgt gcctatgcgt
    46741 gtgaaaaaga cggcatcgcg atgctcaaac gcaactggcg gcgcgtggcg gacaacgccc
    46801 cttccggcac aaatttcgcc ttcaccgaaa atgcgccgcc ccctgccgcc tatcgcagca
    46861 acgccggtca aaccgccgaa tttgccgcaa acagcattcc cgaacgcgga ttccgatttg
    46921 tccgccacac tagctttacc gccttaagcc gccataccca aacgcccgac ggcggcgaag
    46981 aagatgcctg cccgtccttg gatgccgccg aaacctcggt gccggcgatg ccgtctgaaa
    47041 cgccgacggc ttcagacggc atatcgatac acgattttcc gaaaggcacg caggcggggc
    47101 tgtgcctgca cgaaattctt gaagatttca aattcggaca agcagccgcc ggacaggaaa
    47161 ccctcattgc cgacaagctg aaaaaatacg gttttgaaga aatatggctg cccgccgttg
    47221 ccgaaatggc ggaagcctgc cgcaaaacgc cgctgacggg ggcatacgac ctgtccgaca
    47281 tctcacccga gtgccgctgt cccgaaatgg gctttaccct ccacaccgaa gacttcggcc
    47341 tcaaacggct gcgcgactgg tttgcccgcg acgacatcag gctgcccgaa gtctgccgtg
    47401 ccgccgccga aacgctcgac ttccacaccg tcaacggctt tttaaacggc tttatcgaca
    47461 tggtctgcca agaccccgac ggcaatatct gcgtcatcga ctacaagtcg aaccacctcg
    47521 gcacggacgc atccgcctac acgcaacagg caatggacga agccgtcgca caccaccact
    47581 attaccttca ggcgctgatt tacgccgttg ccgccgcacg ctacttcaaa ctgcgcggac
    47641 aaccgcccgc cgccgtttcc gtccgctacc tgtttttgcg cggcacagac ggcaaaggcg
    47701 gcggcgtttg gcgttgggac atagatgccg ccgctttgga acagataaaa taacttcccg
    47761 tcccttaaaa cgacaaccgc aggagaaccc tatgaatatc aaacacctga ttaccgccgc
    47821 actcattgcc tcagcagcct ttgccgcgca ggcagccccg caaaaacccg tatccgccgc
    47881 ccaaaccgcg caacatccgg ccgtttggat cgatgtccgt tccgaacagg aatttagcga
    47941 agggcatttg cacaacgcgg tcaacatccc cgtcgaccaa atcgtccgcc gcatacacga
    48001 agccgcgccc gacaaagaca cgccggtcaa cctctactgc cgcagcggac ggcgtgccga
    48061 agccgccctt caagagctga aaaaagcagg ttatacaaat gttgccaatc acggcggtta
    48121 tgaagacctg ctcaaaaaag ggatgaaatg acgcaatgcc gtctgaaagg acaggctttc
    48181 agacggcata cggcgttccg gcacgcggcg caacatcggg cgcggcgcgt gccgccccgt
    48241 ccgaagcctg ataaacaaac cgaaaaggaa aaaggaaata cgatgaaaca catactcccc
    48301 ctgattgccg catccgcact ctgcatttca accgcttcgg cacatcctgc cagcgaaccg
    48361 tccacccaaa acgaaaccgc tatgaccacg cataccctca cctcaaaata cagttttgac
    48421 gaaaccgtca gccgccttga aaccgccata aaaagcaaag ggatggacat ttttgccgtc
    48481 atcgaccatc aggaagccgc ccgccgaaac ggcttaacga tgcagccggc aaaagtcatc
    48541 gtcttcggca cgcccaaagc cggtacgccg ctgatggtca aagaccccgc cttcgccctg
    48601 caactgcccc tgcgcgtcct cgttaccgaa acggacggca aagtacgcgc cgcctatacc
    48661 gatacgcgcg ccctcatcgc cggcagccgc atcggtttcg acgaagtggc aaacactttg
    48721 gcaaacgccg aaaaactgat acaaaaaacc ataggcgaat aagcccgttg ccgacatatg
    48781 gcggatggaa atcaaacgca cacgcccctc attcccgcga agtcgggaat ccaagcggtt
    48841 ggattgccgt gattttcaat cattgccgaa acggacgatt ccggattccc gccctcgcgg
    48901 ggatgacggg cgtgtgtgtt tttgccacat cacaggcaac tttgccacaa atcttatctg
    48961 tcgccggtat gccttcttgt ttcaatccgc cacaaaccaa atgccgtctg aaacccgatt
    49021 tcagacggca tttccgttta ttccctgctg tcgcggcaaa tatcgatggc ttcctgcaaa
    49081 ctcgaaacgc cgtagatttt caggttcgga aactctttga cgttgcgcgg catattggct
    49141 tttgggacga tggcgcgttt gaagccgagt ttttccgctt ctttgagccg ctcttgcccg
    49201 cgtgcgacgg ggcggacttc accgctcaaa ccgatttctc caaatgcaac cattttttcg
    49261 ggcagcgggc ggttgcggaa gctggaaagc atcgcgagga tgactgccaa atccgccgcc
    49321 ggttcgccga ttttcacgcc gccgacggca ttgagaaaca cgtcttggtc gaaacaggcg
    49381 atgccgccgt gtcggtttaa cacggcaagc agcatcgcaa ggcggttttg ttccagtccg
    49441 acggtaaggc gtttgggggt gaatccgtgc gcgtcatcga ccaatgcctg aatttcaacc
    49501 aaaagcggtc ggctgccttc ctgcgtaacc aaaacgcacg aaccgggcgt gtcgtcgcgg
    49561 tagctggcaa ggaagatggc ggacgggttg gacacgcctt tcaaaccgtt ttcggtcatg
    49621 gcgaatacgc ccagttcgtt tgccgcgccg aagcggtttt tgatggcgcg tatcatgcgg
    49681 tagttggaat gctggtcgcc ctcgaaatac agcacggtat cgaccatatg ctccagcacg
    49741 cgcggaccgg caatcgcacc gtctttggtc acgtgtccga ccagtatcat ggcgatgccc
    49801 atctgtttcg ccatgcgcgt cagttgggcg gcacactcgc gcacctgcga cacggaacca
    49861 ggggcggaag tgatttggtc ggaatacatg gtttggatgg agtcgatgac gacgacttcg
    49921 ggctgatgct gtttcaaggc cgtctgaatg gcttccatgc ggatttcggc aagcaggttc
    49981 acgccttcgg tcggcagttc caaacgttgc gcgcgcaggg cgacctgttg ggcggattcc
    50041 tcgccggaaa cgtacagtac tttgcggctt tgcgccattt tggcgatggt ttgcagcagc
    50101 agcgtggatt tgccgatgcc ggggtcgccg ccgagcagga tgaccgcacc gtcgaccaaa
    50161 ccgccgccca atacgcggtc gagttcgccc atgccggtcg gattgcgcgg cacttcggtg
    50221 gcggtaacgg cggagaggga ttggacggtc gaagtgtccg ccgcccaaga ttggaaacgg
    50281 gcatttttcg gctcgggcgc ggctaagctt tcctgaagtg tgttccactc gccgcaatgc
    50341 gggcatttgc cctgccattt cggggaagtg ccgccgcatt cggtgcattg gtaaagggtt
    50401 ttaagcgttt ttgccatgat gttttcccta tgggattttt gaagtgtgaa aaaatcgtct
    50461 gaaacttctt ttctaaaaaa ttttttatta ttagtttgtt aattttgatt acaagaccat
    50521 atcacgatac aaacaagggt ggctttctgt tattttgtgg cataatgacg gaaaatacaa
    50581 aacaggaatc ccaaacaatg actttcccat taaatataat ggtaga
//
LOCUS       DAWFJW010000002        40727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFJW010000002 DAWFJW010000000
VERSION     DAWFJW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.002"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(175..867)
                     /locus_tag="WGR19_000051"
     CDS             complement(175..867)
                     /locus_tag="WGR19_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0797663.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="WGR19_000052"
     CDS             complement(975..2168)
                     /locus_tag="WGR19_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0797664.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WGR19_000053"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WGR19_000053"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0797665.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="WGR19_000054"
     CDS             complement(3021..3950)
                     /locus_tag="WGR19_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0797666.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WGR19_000055"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WGR19_000055"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0797667.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="WGR19_000056"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="WGR19_000056"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0797668.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLLSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNSQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNATLESGENQAGVGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7342..7881
                     /locus_tag="WGR19_000057"
     CDS             7342..7881
                     /locus_tag="WGR19_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0797669.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8071..8286)
                     /locus_tag="WGR19_000058"
     CDS             complement(8071..8286)
                     /locus_tag="WGR19_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797670.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8294..8497)
                     /locus_tag="WGR19_000059"
     CDS             complement(8294..8497)
                     /locus_tag="WGR19_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797671.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8410..8769)
                     /locus_tag="WGR19_000060"
     CDS             complement(8410..8769)
                     /locus_tag="WGR19_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0797672.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8822..9199
                     /locus_tag="WGR19_000061"
     CDS             8822..9199
                     /locus_tag="WGR19_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0797673.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWSQAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9398..10018
                     /locus_tag="WGR19_000062"
     CDS             9398..10018
                     /locus_tag="WGR19_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0797674.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10041..11492
                     /locus_tag="WGR19_000063"
     CDS             10041..11492
                     /locus_tag="WGR19_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0797675.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFSNVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            11943..12299
                     /locus_tag="WGR19_000064"
     CDS             11943..12299
                     /locus_tag="WGR19_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0797676.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKELGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            12290..12772
                     /locus_tag="WGR19_000065"
     CDS             12290..12772
                     /locus_tag="WGR19_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0797677.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            12785..13378
                     /locus_tag="WGR19_000066"
     CDS             12785..13378
                     /locus_tag="WGR19_000066"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0797678.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDN
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            13368..14624
                     /gene="nuoD"
                     /locus_tag="WGR19_000067"
     CDS             13368..14624
                     /gene="nuoD"
                     /locus_tag="WGR19_000067"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0797679.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            14624..15097
                     /gene="nuoE"
                     /locus_tag="WGR19_000068"
     CDS             14624..15097
                     /gene="nuoE"
                     /locus_tag="WGR19_000068"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0797680.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYNLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCGFMTEEAIEKKLAELE"
     gene            15198..15762
                     /locus_tag="WGR19_000069"
                     /pseudo
     CDS             15198..15762
                     /locus_tag="WGR19_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
     gene            15922..17223
                     /gene="nuoF"
                     /locus_tag="WGR19_000070"
     CDS             15922..17223
                     /gene="nuoF"
                     /locus_tag="WGR19_000070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0797681.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFESALAQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVYYIEHGGPMKEHKWGGW"
     gene            17223..17660
                     /locus_tag="WGR19_000071"
     CDS             17223..17660
                     /locus_tag="WGR19_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797682.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            17950..20211
                     /locus_tag="WGR19_000072"
     CDS             17950..20211
                     /locus_tag="WGR19_000072"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0797683.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAVQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTVAI
                     LKDALDAESLPSKLNNRAASTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQATSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGAGVSVMVKGDAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            20214..21290
                     /gene="nuoH"
                     /locus_tag="WGR19_000073"
     CDS             20214..21290
                     /gene="nuoH"
                     /locus_tag="WGR19_000073"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0797684.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            21371..21850
                     /gene="nuoI"
                     /locus_tag="WGR19_000074"
     CDS             21371..21850
                     /gene="nuoI"
                     /locus_tag="WGR19_000074"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0797685.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            21862..22533
                     /locus_tag="WGR19_000075"
     CDS             21862..22533
                     /locus_tag="WGR19_000075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0797686.1"
                     /translation="MTFSAILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            22530..22835
                     /gene="nuoK"
                     /locus_tag="WGR19_000076"
     CDS             22530..22835
                     /gene="nuoK"
                     /locus_tag="WGR19_000076"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0797687.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            22869..23444
                     /locus_tag="WGR19_000077"
     CDS             22869..23444
                     /locus_tag="WGR19_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0797688.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            23568..23909
                     /locus_tag="WGR19_000078"
     CDS             23568..23909
                     /locus_tag="WGR19_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797689.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            23958..25982
                     /gene="nuoL"
                     /locus_tag="WGR19_000079"
     CDS             23958..25982
                     /gene="nuoL"
                     /locus_tag="WGR19_000079"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0797690.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAVPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSARLVGAVAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            26038..26344
                     /locus_tag="WGR19_000080"
                     /pseudo
     CDS             26038..26344
                     /locus_tag="WGR19_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
     gene            26419..28134
                     /locus_tag="WGR19_000081"
     CDS             26419..28134
                     /locus_tag="WGR19_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ0797691.1"
                     /translation="MANLNVSSKKIINLLGNQDMQEKYFIIPEYQRPYRWDTEKCAVL
                     WSDIVDFRQSSTGKNEEYFLGTIVVCPNEKGNLEVIDGQQRLTSLLLLLRAFYRVLEQ
                     TESTPDTAAKITTLKSKIAPCIWDVDVLTAEITDRTAIRLHTQVATETDNEILHEILA
                     NGEISEECRSNYAVNYLYFLKQCQEYARTNPMDWYHLCLTILNDCILLPIESDSLNSA
                     LTIFSTLNDRGLPLSDADIFKARLYKMAQQKDKFVEQWKELVALAEGASISVDDLFRY
                     YTHVLRAKEKITDKEIGLRRFYTDNRNKQLENENLFNHLIELAGFWQDINNSKTNQDV
                     ETSNFVNDEAAKWLQCLNVYPNEFWKYAASVFFIANKGAQSFKDDFAALLKQLTAFLY
                     AKFLVAPTVTAIKDDIYKLCVAIYHGEKNALSYVMPAPIHYEQNTGRITKGLLMINAY
                     LFDEKQKLLPTKTEIEHIFPKKWQETNYNGWSIIDAEQYLEQLGNKIVFEKRLNIQAG
                     NGYFGKKKEQYQKSDIVEVRELAGENANDWLPSDIQKRQQQIVDRLKQFFDNCFEAAS
                     IPKKN"
     gene            28171..29667
                     /locus_tag="WGR19_000082"
     CDS             28171..29667
                     /locus_tag="WGR19_000082"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0797692.1"
                     /translation="MFSNHLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            29677..31122
                     /gene="nuoN"
                     /locus_tag="WGR19_000083"
     CDS             29677..31122
                     /gene="nuoN"
                     /locus_tag="WGR19_000083"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0797693.1"
                     /translation="MNWSDLNLMPALPEIALLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSTGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVIMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            31234..31533
                     /locus_tag="WGR19_000084"
     CDS             31234..31533
                     /locus_tag="WGR19_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0797694.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(31642..32538)
                     /locus_tag="WGR19_000085"
     CDS             complement(31642..32538)
                     /locus_tag="WGR19_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0797695.1"
                     /translation="MNPTNDLKTWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(32522..32746)
                     /locus_tag="WGR19_000086"
     CDS             complement(32522..32746)
                     /locus_tag="WGR19_000086"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0797696.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(33008..33931)
                     /gene="rsgA"
                     /locus_tag="WGR19_000087"
     CDS             complement(33008..33931)
                     /gene="rsgA"
                     /locus_tag="WGR19_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0797697.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PSCAFKAAAETGAASPERLAFLQGITDELPG"
     gene            complement(33936..35792)
                     /locus_tag="WGR19_000088"
     CDS             complement(33936..35792)
                     /locus_tag="WGR19_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0797698.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(35818..35955)
                     /locus_tag="WGR19_000089"
     CDS             complement(35818..35955)
                     /locus_tag="WGR19_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797699.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(35955..36539)
                     /gene="ruvA"
                     /locus_tag="WGR19_000090"
     CDS             complement(35955..36539)
                     /gene="ruvA"
                     /locus_tag="WGR19_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0797700.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(36621..36947)
                     /locus_tag="WGR19_000091"
     CDS             complement(36621..36947)
                     /locus_tag="WGR19_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0797701.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(37001..37720)
                     /locus_tag="WGR19_000092"
     CDS             complement(37001..37720)
                     /locus_tag="WGR19_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0797702.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASASSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(37717..37839)
                     /locus_tag="WGR19_000093"
     CDS             complement(37717..37839)
                     /locus_tag="WGR19_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0797703.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(37850..38314)
                     /gene="trmL"
                     /locus_tag="WGR19_000094"
     CDS             complement(37850..38314)
                     /gene="trmL"
                     /locus_tag="WGR19_000094"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0797704.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(38379..38987)
                     /locus_tag="WGR19_000095"
     CDS             complement(38379..38987)
                     /locus_tag="WGR19_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0797705.1"
                     /translation="MAAYRTDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHY
                     EPPVSNMIRTLKHLADLGMVRPLANLMVQNPPDRLADECFDFVLPVPLSRERLLQRGF
                     NQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKGSERRRNIKNAFEIRTPIPENC
                     NILLIDDVFTTGATLDELAKTLKKSGANRICCWTLARTPMKK"
     gene            38971..39846
                     /gene="bioH"
                     /locus_tag="WGR19_000096"
     CDS             38971..39846
                     /gene="bioH"
                     /locus_tag="WGR19_000096"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0797706.1"
                     /translation="MRYAAKSAAQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKF
                     MPDAAKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDAPFAQPFDIEAAAD
                     AVAAQIDTPADILGWSLGGLVALYLAARHPGKVRSLCLTASFARLTADEDYPEGLAAP
                     ALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAA
                     ERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSH
                     AEAFAALYRDFVEGV"
     gene            39848..40636
                     /locus_tag="WGR19_000097"
     CDS             39848..40636
                     /locus_tag="WGR19_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0797707.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         9399 a        10304 c        10916 g        10108 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgtgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcaac gtacttgggc gcgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaagccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagactg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tacctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 ttagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg ttcactgctt tgacccgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttggt
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgtgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagacaatca cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt acccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggtg aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggtgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catctctatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcccttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggctgttg caaaccggtc aggcaagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc aaacccgact cggcgcgatg attcccatca acgtccgcgc
     5041 catgtggctc ggcacgttcc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgccggtctg ctgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggctt gcgcgcttcc gtcttgagcg cgcccgaccc
     5281 gcacacaagc cgcatgatcg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccaa cactaccaaa accgcttcaa tcacattctg gtcgacgaat tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggcaacgcgg cggtatttgc
     5521 cgtcggcgac gacgaccaaa gcatctaccg tttccgtggc gcaaacgttg gcaatatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atcctcgccg ccgccaacgc cgttatcgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatc cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg cagcaactcc caatcgcgcg tcatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct tttacgaacg
     5941 ccaagaaatc aaacacgccc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcctcaaacg aacaaggcat caccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaagtcg tcgccttcgt ccgcctgatt gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgtccg aaatcatcgt cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgcctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgagttca aaccagaaga cagcaacttt gaaatcctgc ctgaaaacat
     6361 ttcagacgat cccgccttcc ccatcctcgc tttcttgagc aacgccaccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgtgg gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggtatggaa gaaggccgtt tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagcctg cctacgacag ttacggcaac acgcgccaaa ccgccgcatc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aatttggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggacaccaa
     6961 gtttgcgaaa ttggaagaga tgtaaatttg aaatgcaggc tggatactcg tatccgacaa
     7021 aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc ggattcgaga
     7081 atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct gaatccggta
     7141 acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat ccagaccttt
     7201 cggcacggga acttatcgga taaaaggttt ctttagattc cacgttctag attcccgcct
     7261 gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt ttatagctga
     7321 aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt gtcctccgta
     7381 tgggaatccg acagttgggc agatgaaaac tacaaagaag ccccgtttac ccctgaaatt
     7441 ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat tgaattgatg
     7501 gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacaca gagaaattcg
     7561 tgggcgaaag atcatgtgca aatttgcgag gtatcgggca tcggctttga aaaagaaggg
     7621 agtttgtgcg gcgaaacagg gcaaaaactt tggatggaag aatgggaata tccgcccatc
     7681 ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgcca tgtttgcctt ggactatcgg
     7741 gagtgtggaa aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc ggattttgaa
     7801 atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca tgaagatgag
     7861 tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa gggaaaacgg
     7921 gcggaataac agataaccga ttgaatccgt aaggaaaacg gtttggtgga acgcgccatc
     7981 caagaccttt gcaaagaaaa actgtctccg acagcattga cattattaac agaacttatc
     8041 aagtttggag ctatctcaaa tataattcgg ttatcctttt gtatccatta aatcatatgc
     8101 ttcaattaat tgttgttcta gctcttgtac caattttgaa ttgcgaattc ctgacacaat
     8161 ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa gggaacgcaa
     8221 taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt aattgattat
     8281 attcataata attttatttt tgtttgtcaa agtaagtttt gcctaaggtt ggtctaaatg
     8341 cagttccacc atcttttgaa tttgggtctc tgattacaat tgctccagac ttatcatccc
     8401 aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa atcgtaataa
     8461 gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt tatcaatttc
     8521 acttttaata atcttatgat caaactgttc taatattaat gcattagacc aaaaaaaacc
     8581 ttctttatta caatgatggg aaatccattt aggagaacaa atgcaaagtg aaaaaataga
     8641 tgagccttgt tctccttcga ttccgatatc caaatctatc catctatgga aattatctgg
     8701 aatttcgggg gtaaattttt caaaatcaat atcatataaa tttatgcttt ttaaatccaa
     8761 tttaatcatt agggctgtcc tagataaata gggaaattca aattaagtta gaattatccc
     8821 tatgagaaaa agtcgtctaa gccggtataa acaaaataaa ctcattgagc tatttgtcgc
     8881 aggtgtaact gcaagaacag caacagagcc cgacagcatt gtttatacgg attgttatcg
     8941 tagctatgat gtattagatg tgagcgaatt tagccatttc cgtatcaatc acagcacaca
     9001 ttttgctgaa cgacaaaacc atatcaatgg aattgagaat ttttggagcc aggcaaaacg
     9061 tcatttacgc aagtttaacg gcattcccaa agcgcatttt gagctgtatt taaaggagtg
     9121 cgaatggcgt tttaacaaca gtgagataaa agttcaaatt tccattttaa aacaattagt
     9181 aaaatcgagt ttatcttagt tgtccaggac agccccatta tttttataac accgtgaagc
     9241 cgcacagcag tttgaacagt gatacgccgt ttgcgggctt acgagtttat tttcccggcc
     9301 tgcagtttga gcaatacggt gatttcctac ggttaataca aatgtttaca cattgataca
     9361 tttcatttat agttccgcct atttgaaaat agaaaatatg aattcgaccg caagtaaaac
     9421 cctgaaagga ttgtcgctgg tgtttttcgc ctctggattc tgcgccctga tttaccaggt
     9481 cagctggcag aggcttctat tcagtcacat aggtatcgat ttgagttcga ttactgtcat
     9541 tatttctgta tttatggtcg gcttgggtgt aggtgcgtat ttcggtggac gcattgctga
     9601 ccgttttcct tcaagtatca tccccctgtt ttgcatcgct gaagtatcca tcggtctgtt
     9661 cggtttggta agcaggggtc tgatttccgg cttggggcat cttttagttg aggctgattt
     9721 gcccatcatc gctgctgcca atttcctctt attgctgctt cctaccttta tgatgggcgc
     9781 gaccttgccc ttgctgacct gtttttttaa ccggaaaata cataatgttg gcgagtctat
     9841 cggtacctta tattttttca acactttggg tgcggcactc ggatcgcttg ccgccgccga
     9901 atttttctac gtctttttta ccctctccca aaccattgcg ctgacagcct gctttaacct
     9961 tctgattgct gcttcagtat ggctgcgtta cagaaaggat ggatatagtg aacactaaac
    10021 cgaatactag tttgatttat atgctttctt tccttagcgg cttattgagc ttgggtatag
    10081 aagtcttgtg ggtaaggatg ttttcgttcg cagcacagtc cgtgcctcag gcattttcat
    10141 ttacccttgc ctgttttctg accggtatcg ccgtcggcgc gtattttggc aaacggattt
    10201 gccgcagccg ctttgttgat attcccttta tcgggcagtg cttcttgtgg gcgggtattg
    10261 ccgacttttt gattttgggt gctgcgtggt tgttgacggg tttttccggc ttcgtccacc
    10321 acgccggtat cttcattacc ctgtctgccg tcgtcagagg gttgattttc ccgctcgtac
    10381 accatgtggg tacggatggc aacaaatccg gacgacaggt ttccaatgtt tatttctcca
    10441 acgttgccgg cagtgcattg ggtccggtcc ttatcggctt tgtgatactt gatttcttgt
    10501 ccacccaaca gatttacctg ctcatctgtt tgatttctgc tgctgtccct ttgttttgta
    10561 cactgttcca aaaaagtctc cgactgaatg cagtgtcggt agcagtttcc ctaatgttcg
    10621 gcatcctcat gttcctactg ccggattctg tctttcaaaa tattgctgac cgtccggata
    10681 ggctgattga aaacaaacac ggcattgttg cggtttacca tagagatggt gataaggttg
    10741 tttatggggc gaatgtatac gacggcgcat acaataccga tgtattcaat agtgtcaacg
    10801 gcatcgaacg tgcctatctg ctaccctccc tgaagtctgg catacgccgc attttcgtcg
    10861 ttggattgag tacaggttcg tgggcgcgcg tcttgtctgc cattccggaa atgcagtcga
    10921 tgatcgttgc ggaaatcaat ccggcatacc gtagccttat cgcggatgag ccgcaaatcg
    10981 ccccgctttt gcaggacaaa cgtgttgaaa ttgtattgga tgacggtagg aaatggctgc
    11041 gtcgccatcc tgatgaaaaa ttcgacctga ttttgatgaa tacgacttgg tactggcgtg
    11101 cctattccac caacctgttg agtgcggaat ttttaaaaca ggtgcaaagc caccttaccc
    11161 cggatggtat tgtaatgttt aataccacgc acagcccgca tgcttttgct accgccgtac
    11221 acagtattcc ctatgcatac cgctatgggc atatggtagt cggctcggca accccggtag
    11281 ttttccctaa taaagaactg ctcaagcaac gtctctcccg gttgatttgg ccggaaagcg
    11341 gcaggcacgt atttgacagc agcaccgtgg atgctgcagc acaaaaggtt gtctctcgta
    11401 tgctgattca gatgacggaa ccttcggctg gggcggaagt cattaccgac gataatatga
    11461 ttgtagaata caaatacggc agagggattt aaccgtctta aagggtttca ggcaacgcag
    11521 gttttaggta acgtcctgct agttcaaaaa aaccgcatca cagcagtcgg gacaaaatgg
    11581 tttaaacatt ttgtcccgaa ttcttattcc tatatatagt ggattaacaa aaatcaggac
    11641 aaagcgacga agccgcagac agtacaaata gtacggaacc gatttcagca ccttagagaa
    11701 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactatacc
    11761 acgaattacg gtgtaaaaat ttatatgacc ttataaaatc aaataagaat cgttatcata
    11821 acatgattgt atttattggg tttttttggg cgttttgccg atatttacct tttaatggtt
    11881 tttgaaattc gctaaaatac gaaattattg tagaaatttt gttaacggat ttgggtgtaa
    11941 ccatgttgtc cgcttacttt cccgtctttg tctttatcct cgtcggcctc gcggccggcg
    12001 tgctgtttat cctgctcggc acgattttag gcccgaaacg ccactacgcc gaaaaagacg
    12061 cgccttacga atgcggtttt gaagcctttg aaaacgccag gatgaagttc gacgtgcgct
    12121 attacctcgt cgccatcctc tttatcttgt ttgatttgga ggtcgcgttc atgttgccgt
    12181 gggcagtggt gtttaaagaa ttgggcgcgt acggcttctg gtctatgctg gtgtttatcg
    12241 tcgttctgac ggtaggcttt gtttacgaat ggaaaaaagg tgcgctggaa tgggaataga
    12301 aggcgttttg aaaaaaggtt tcatcaccac cagcgcggat acggtgttga actatatgcg
    12361 taccggttca ttgtggccgg ttactttcgg cttggcctgc tgcgccgtgg aaatgatgca
    12421 tgcgggtatg gcgcgttatg accttgaccg tttcggtatc attttccgtc cgtccccacg
    12481 tcaggccgac ctgatgattg tggcgggtac gctgaccaac aaaatggcgc ccgccctgcg
    12541 ccgcgtatac gaccagctcg ccgagccacg ctgggtattg tctatgggct cgtgtgccaa
    12601 cggcggcggt tattatcact attcttattc cgttgtgcgc ggtgccgacc gcgtcgtgcc
    12661 ggtagacgtt tatgtgccgg gttgtccgcc gactgcggaa gccctgattt atggcctgat
    12721 tcagctccaa caaaaaatca agcgcacttc caccatcgcg cgtgacgagt aaggagagga
    12781 cgatatggca agcattcaaa acttatacga aaccgtcagc cgcgttttgg gcaatcaggc
    12841 aggcaaagtc atttccgctt tgggcgagat taccgtcgag tgtctgcccg aacactatat
    12901 ttcagtcatg accgcattgc gcgaccatga agagttgcat ttcgagcttc tggtcgactt
    12961 gtgcggtgtc gattacagca cttacaaaaa cgaagtatgg cagggcaaac gctttgccgt
    13021 cgtcagtcag ttgctttccg ttaaaaacaa tcaacgcatc cgcgtacgcg tctgggtttc
    13081 agacgacaac ttccccgtag tcgaatccgt cgtcgatatt tacaacagcg cggattggta
    13141 cgaacgcgaa gcctttgata tgtacggcat catgttcaac aaccatcccg acctgcgccg
    13201 catcctgacc gattacggtt tcgttggaca tccgttccgc aaagacttcc cgatttccgg
    13261 ctatgtggaa atgcgttacg acgaagagca aaaacgcgtg atttaccaac ctgttaccat
    13321 cgagccgcgc gaaatcacgc cgcgtatcgt ccgtgaggag aactacggtg gccaataaat
    13381 taagaaacta caccatcaac ttcggcccgc aacaccctgc ggcgcacggc gtattgcgta
    13441 tgattttgga gttggaaggc gaaaccatcg tccgtgccga cccgcatatc ggtctcctgc
    13501 atcgcggcac cgaaaaactg gcggaaacca aaacctacct gcaagccctg ccttatatgg
    13561 acaggctgga ctacgtttcc atgatggtca acgagcaggc gtattgcttg gcggtagaaa
    13621 aacttgccgg tatcgatgta cctattcgcg cccaatacat ccgcgtgatg ttcgccgaag
    13681 taacgcgcat cctcaatcac ttgatgggca tcggttcgca cgccttcgac attggcgcga
    13741 tgaccgccat cctctacgcc ttccgcgacc gcgaagagct gatggacttg tacgaagccg
    13801 tttccggcgc gcgtatgcac gccgcctact tccgtcccgg cggcgtttac cgcgatctgc
    13861 ccgacttcat gcccaaatac gagagcagca aattccgcaa cgccaaagta ttgaagcagc
    13921 tcaacgaatc ccgcgaaggc accatgcttg actttatcga tgccttctgc gaacgcttcc
    13981 ccaaaaatat cgacacactc gaaaccctcc tgaccgacaa ccgtatttgg aaacaacgta
    14041 ccgtcggcat cggcgtcgtc tcccccgagc gtgccatgca aaaaggcttt accggcgtga
    14101 tgttgcgcgg ttcgggtgtg gaatgggacg tgcgtaagac acagccttac gaagtgtacg
    14161 acaaaatgga tttcgacatc cctgtcggcg tgaacggcga ctgctacgac cgctacctct
    14221 gccgtatgga agaaatgcgt caatccgtac gcatcatcaa acaatgttcc gagtggttgc
    14281 gtgtcaatcc gggtccggtc attaccacaa accacaaatt cgctccgccc aaacgtaccg
    14341 aaatgaaaac aggtatggaa gacctgattc accatttcaa actctttacc gagggtatgc
    14401 acgttcccga gggcgagacc tacaccgctg tcgaacatcc gaaaggcgag ttcggcgttt
    14461 acatcatttc agacggcgca aacaaaccct accgcctgaa aatccgcgca cccggcttcg
    14521 cccatctgca aggcatggac gaaatggcaa aaggccacat gctggccgac gtcgttgcca
    14581 tcatcggtac gcaggacatc gtattcgggg aggttgaccg ataatgttat ccgcagaatc
    14641 cttaaaacaa attgatattg agttggcaaa atatcctgcc gaccaacgcc gctccgcgat
    14701 tatgggcgca ttgcgtatcg cccaaaccga aaaaggctgg ctcgcccccg aaaccatcgc
    14761 ctttgttgca gactatatcg gcatctcgcc tgcacaagcc tacgaagtcg ctactttcta
    14821 caatatgtac aaccttgagc ctgtcggcaa atacaaactg accgtttgta ccaacctgcc
    14881 ttgcgccctg cgcggcggta tggctaccgg cgaatacctc aaacaaaaac tcggtatcgg
    14941 ctacggcgaa accacgcccg acggtaaatt tacccttgtc gaaggcgaat gtatgggcgc
    15001 atgcggcgac gcgccggtga tgctggtcaa caaccacagc atgtgcggct ttatgactga
    15061 agaggcgatt gagaaaaaat tagcagaatt agaatagatt gtccgaacac attaggtttg
    15121 taatcaaact taaccggtta ttccaaaatt ttaaattgtg attttaaaaa gtaacaataa
    15181 aaaaggggga gtgcttaatg agaggggtaa tcgtcaagtg ggatgacgaa aaaggttatg
    15241 gttttatacg gatagatggc ggtaacaaag aaattttctg ccatatttct gacttttccc
    15301 caagaaaccc gcgtcctgag attgacgagg cggtcggttt tgaagtggtt tcagatggca
    15361 agggaagatt ttctgcaaaa cagatacgct atctgaatcg ggagcgtgca gtttctcccg
    15421 tataccgcaa gaaacatcga aaagacgagg aatctcccac ttcgattgtt caggttgtgc
    15481 tgtctttgtc ggtcggtttg ctgcttgttt cggtaatcgg ctataaggcg taccaatatg
    15541 cgtcagagaa gctgactccg aaacaggaaa atacgccggt taaaacgcaa gtattacccg
    15601 aaataaaacg gcagcagccc ataccgttgc gacggcaggc aacattgctc acaaatgact
    15661 tcgtgcgaag aagccaaatg gtttgtaagg aactgttccg ataccaagat ggacggcgat
    15721 ggcgacggca ttccgtgtga agacaaatgc ggcggatggt aattttgatt gatgacggga
    15781 agaacaaacg caggtttggt tcagattaaa cgaagacagg aaaatgccgt ctgaaaacag
    15841 acaaaccgtt tcagacggca tcaggaacat tgagacggca ggcagccgca aacgatacga
    15901 aaacaaacag gcacaccaaa aatggctatt taccaatcag gcgtgatttt tgaccaagtg
    15961 gataccgcca atcccgattg ctggacattg gacgaatacg tcaaacgcgg cggttatacc
    16021 gccctgcgta aaatcctgtc cgaaaacatc tcgcaaaccg atgtgattga cgaagtcaaa
    16081 acctccggct tgcgtggtcg tggcggtgcg ggcttcccga ccggtttgaa atggagcttt
    16141 atgccccgtt ctttcccggg cgaaaaatat gtggtttgca acaccgacga aggcgagccg
    16201 ggtacattta aagaccgcga catcattatg ttcaaccccc atgccttaat tgaaggcatg
    16261 attatcgccg gttacgcgat gggcgcaaaa gcgggctaca actatatcca cggcgaaatt
    16321 ttcgaaggct accaacgttt tgaatccgct ttggcgcagg cgcgcgcggc aggctttttg
    16381 ggtaaaaata ttttgggttc ggacttcgaa ttcgaacttt tcgcccacca cggctacggc
    16441 gcatatattt gcggtgaaga aaccgcattg ctcgaatcgt tggaaggcaa aaaaggccag
    16501 ccgcgcttta agccgccatt ccctgcttcg ttcggtctgt acggcaaacc gaccaccatc
    16561 aacaataccg aaacgttctc ctcagtcccc ttcattatcc gtgacggcgg acaggcattt
    16621 gccgataaag gtattccgaa tgcaggcggt accaaattat tctgtatttc cggtcatgtc
    16681 gagcgtccgg gcaactatga agtgccattg ggtacgccgt ttgccgaagt cttgaaaatg
    16741 gcaggcggta tgcgcggcgg taaaaaactc aaggccgtta ttcccggcgg ttcgtccgcg
    16801 cccgtattgc ctgccgacat catgatgcag accaatatgg actatgactc catttccaaa
    16861 gcaggctcga tgctcggttc cggcgcgatt atcgtgatgg acgaagacgt gtgcatggtc
    16921 aaagccctcg aacgcttaag ctacttttac tacgacgaat cctgcggcca atgcaccccg
    16981 tgccgcgaag gtacaggctg gctctaccgc atcgtccacc gcatcgtaga aggcaaaggt
    17041 aaaatggaag atttggattt gctggattcc gtcggcaacc aaatggcagg ccgcaccatc
    17101 tgcgcccttg ccgatgccgc tgttttccct gttcgcagct ttaccaagca tttccgtgat
    17161 gagtttgtgt attacatcga acacggcggg ccgatgaaag agcataagtg gggagggtgg
    17221 taatggtgga agctaaaatt tttattctat acggtgcagc caacaaaggt aagagtacga
    17281 cactcaatac gctttttaat cagatttgtt ggaaattttc taaatttcta gtcttttttg
    17341 aaagacatgg aaatggctta gattttgttg cagtatttga tcatgaaggt cagagaattg
    17401 gtttttattc atctggtgat aatgaatacg aggttaggag aaatttatac aaactttatt
    17461 cgcataattg tgattttatt tttggcacgt caaggacacg gggtggtagt tgcgatgcag
    17521 taggatgtta tgcagagtta ttgcatggcg atgtaaatat aattaattgg tgtgaaaagt
    17581 ttgagcctac agatgaagac aatgagcgtg ctgttaaaga gttatttaag tcatttaaaa
    17641 atataataaa tgagttatag ttttagttgg ttttatattg gttaaaagca aaatgctaaa
    17701 aatttaactt tgccgtcatt cccgcgtagg cgggaatcca tagtggaatt tacagaaccc
    17761 gatatttgaa aagcagttgc cgaaattcaa aaaatggatt cccgcctacg cgggaatgac
    17821 ggcaaggtgg cagtaaacgt tttaaacagt attgattgtc aatgaaactc aaaaggccgt
    17881 ctgaaaccca tttttcagac gacctccata aaagattatt tatcaaatac ccgtaactag
    17941 gaacgaacca tgttacaaat cgaaatcgac ggcaaacagg tatctgtgga gcagggtgcg
    18001 acggtgattg aagccgcgca caagctcggt acttatatcc cgcatttctg ttaccacaaa
    18061 aaactttcca tcgccgccaa ctgccgtatg tgtttggtgg acgtggaaaa agctcccaaa
    18121 cctctgcccg cctgtgccac gccggtaacg gacggcatga ttgtgcgtac gcattcggca
    18181 aaagcccgag aggcgcaaga aggtgtgatg gagttcctgc tcatcaacca tccgcttgat
    18241 tgtccgacct gcgaccaagg cggcgaatgc cagttgcagg atttggcggt gggctacggt
    18301 aaaaccacca gccgctacac cgaagaaaaa cgttccgtcg tcggcaaaga catggggcct
    18361 ttggtttccg ccgaggaaat gagccgctgc atccactgta cccgttgcgt ccgtttcact
    18421 gaagaaatcg ccggcttgca ggaaattgcg atggtgaatc gcggcgagca ttctgagatt
    18481 atgcccttta tcggcaaagc ggtggaaacc gaattgtcgg gcaacgtcat cgatttgtgt
    18541 cccgtcggcg cgctgaccag caaaccgttc cgcttcaacg cgcgtacttg ggaattgaat
    18601 cgccgcaaat ccgtttccgc tcatgacgct ttgggcagta acctgattgt acaaaccaaa
    18661 gaccataccg tccgccgcgt gttgccgctg gaaaacgaag cgattaacga atgctggctg
    18721 tccgaccgcg accgttttgc ctacgaaggc ctgtatcacg aaagccgtct gaaaaacccg
    18781 aaaatcaaac agggcggcga gtggatggac gtggattgga aaaccgcatt ggaatatgtc
    18841 cgcagcgcga ttgaatgtat cgccaaagac ggcaaccaaa accaagtcgg catttgggcg
    18901 aacccgatga atacggttga agagctgtat ctggccaaaa aacttgccga cggcttgggt
    18961 gttaaaaact ttgcaacccg tttgcgtcaa caagacaaac gtctttcaga cggcattaag
    19021 ggtgcgcaat ggttgggaca aagcattgaa tctttggctg acaacgatgc cgtattggta
    19081 gtcggcgcga acttgcgcaa agaacagccg ctcctgaccg cccgcctgcg ccgtgccgcc
    19141 aaagaccgta tggcattgag cgtattggcc ggcagcaagg aagagttgtt gatgccgctt
    19201 ctctctcagg aagccgcaca tcccgacgag tgggcaggcc gtctgaaaaa cttgtctgcc
    19261 aatgcggaac acgccgttac cgccagcctg aaaaacgctg aaaaagcagc ggtgattttg
    19321 ggcgcggaag tgcaaaacca tcctgattac gccgcgattt atgccgccgt tcaagagctg
    19381 gcagatgcga ccggcgcagt gctgggtatt ttgccgcaag ccgccaacag cgttggtgcg
    19441 gatgtcttgg gcgtgaactc gggcgagagc gttgccgaaa tggcaaacgc gccgaaacag
    19501 gcagtcttgc tgctcaacgt cgagcctgaa atcgatacgg ttgacggtgc aaaagccgtg
    19561 gctgcgttga aacaagcgaa aagcgtgatg gcgtttacgc cgtttgtcag cgaaacgctg
    19621 ctggacgtgt gcgacgtatt gttgccgatt gcgccgttta ccgaaacctc gggcagcttc
    19681 atcaatatgg aaggccgtct gcaatctttc cacggcgtgg tacaaggctt cggcgattcg
    19741 cgtccgatgt ggaaagtgtt gcgcgtattg ggcaacctgt ttgacctgaa aggttttgaa
    19801 taccacgata ccgtcgcgat tctgaaagac gcgctggatg cggaaagcct gccgtccaaa
    19861 ctgaacaacc gcgccgcttc gactcaaaaa gattttcaga cggcatcaag ccgcctcgtg
    19921 cgcgtgggcg gagtgggcat ctatcacacc gatgccatcg tgcgccgttc cgcgccgctg
    19981 caagcgacca gccatgccgc cgtgcccgcc gcgcgagtga atccgaacac tttggcgcgc
    20041 ttaggcttgc aagacggaca aaccgccgtc gccaaacaaa acggcgcagg cgtgtccgtg
    20101 atggtcaaag gcgatgccgg actgcctgaa aacgtggtgc atctgccgct gcataccgaa
    20161 aatgccgcgc tgggtgcgtt gatggacact attgaactgg cgggagcttg attatgcagg
    20221 aatggttcca aaacctcttt gccgcaacgc tcggtctggg cgatttgggc atcaccgtag
    20281 gcttggtggt atccgtcatc gtcaaaatcg tgattatcct gattccgctg attctgaccg
    20341 ttgcctacct gacgtatttc gaacgtaaag tcatcggctt tatgcagctt cgtgtcggcc
    20401 cgaacgtaac cggcccgtgg ggtctgattc agccgtttgc cgacgtgttc aaactcttgt
    20461 ttaaagaagt tacccgtccg aagctgtcaa acaaagccct gttctatatc ggtccgatta
    20521 tgtcgcttgc tccgtctttt gcggcgtggg cagtgattcc gttcaacgaa gaatgggtgc
    20581 tgaccaacat caatatcggc cttttgtata tcctgatgat tacctcgctg tcggtttacg
    20641 gcgtgatcat cgcgggctgg gcttccaact ctaaatattc gttcttgggc gcaatgcgtg
    20701 cttccgcgca aagcatttcc tacgaaatcg ccatgagtgc cgcgctggtg tgcgtcgtga
    20761 tggtgtcggg cagcatgaac ttctccgaca tcgttgccgc acaagccaaa ggtattgccg
    20821 gcggttcggt attctcttgg aactggctgc cgctgttccc catcttcatt gtttatctga
    20881 tttccgccgt tgctgaaacc aaccgcgcgc cgtttgacgt agcagagggc gagtccgaaa
    20941 tcgttgccgg acaccacgtc gaatattccg gctttgcgtt cgcgctgttc ttccttgccg
    21001 aatacatttt catgattctg attgccgcgc tgacatcgct gatgttcctc ggcggctggt
    21061 tgtctccgtt cccgcaaagc tggggcattg tcggtacgcc ttccgcattc tggatgtttg
    21121 tgaaaatggc ggcagtgttg tactggtacc tgtggatccg tgcaaccttc ccgcgctacc
    21181 gttacgacca aatcatgcgt ttgggctgga aagtgctgat tccgatcggc ttcgcctaca
    21241 tcgtggtttt gggcgtgtgg atgatttcac cgctgaattt gtggaaataa gtttcagacg
    21301 gcatcttgag gccgtctgaa caaagcgatt ttgaatacct aacgaaatcc ctgttttgag
    21361 ggaacataat atggctaact tagtaaaaac tttcttgctc ggcgaattgg taaaaggtat
    21421 gggtgtaacg ctcaaaaact tttttgcccg caaagacaca atttatttcc ccgaagagaa
    21481 aacgccgcaa tccgtgcgtt tccgtggcct gcacgcgcag cggcggtatc cgaacggcga
    21541 agagcgctgt atcgcgtgta aattgtgtga agccgtttgc cccgcaatgg cgattaacat
    21601 tgaatcggaa gaacgcgaag acggcacccg ccgcaccaag cgttacgata tcgacctgac
    21661 caagtgcatc ttctgcggtt tctgcgaaga ggcctgcccg accgatgcga ttgtggaaac
    21721 gcatattttt gaataccacg gcgagaaaaa aggcgacttg cacatgacca agccgatcct
    21781 cttggcaatc ggcgacaaat acgaagccga aatcgccaaa cgcaaagccg ctgacgcgcc
    21841 gtaccgttaa ggagcagacg aatgactttt tccgcgattc tgttctatat ccttgccgcc
    21901 atcgttttgt acggagcggt tcgtaccgtt acagctaaaa accctgtcca cgccgctttg
    21961 catctggtgc tgaccttctg cgtgagcgcg atgatttgga tgctgatgca ggccgagttt
    22021 ttgggtgtga cgctggtggt ggtttacgtc ggcgccgtga tggtgttgtt cctgttcgtc
    22081 gtgatgatgc tgaacatcga catcgaggaa atgcgcgccg gtttttggcg gcacgcgccg
    22141 gttgccggcg tggtcggtac attgttggcg gttgccctga ttctgatttt ggtcaacccg
    22201 aaaaccgacc tggccgcatt tggtctgatg aaagacattc ccgccgatta caacaatatc
    22261 cgcgatttgg gcagccgtat ttataccgac tacctgttgc cgtttgaatt ggcggcggta
    22321 ttgctgctgt tgggtatggt agcggcgatt gcgctggttc accgcaaaac caccaatccg
    22381 aaacgtatgg atcctgccga ccaagttaaa gtacgcgccg accaaggccg tatgcgcttg
    22441 gtgaaaatgg aagccgtgaa accgcaaacc gaatctgccg aagaaagcga agtttcagac
    22501 gacctcaagc cgaaagagga gggcaaagca tgattacctt gacgcattat ctggtattgg
    22561 gtgcgctcct gttcggtatc agcgcgatgg gtatcttcat gaaccgcaaa aacgtgctgg
    22621 tattgttgat gtccattgag ctgatgcttt tggcggtgaa cttcaacttt atcgcctttt
    22681 cgcaacattt gggcgatact gccggacaaa ttttcgtatt tttcgtactg accgttgctg
    22741 ctgccgaatc tgccatcggt ttggcaatta tggtgctggt gtaccgcaac cgacaaacga
    22801 ttaacgttgc cgatttggac gagttgaaag ggtaaaggta ggttgggtcg agacctgaca
    22861 agacaccgat gccgtctgaa aacccgatag gaaaaacgat gaaatccata gacgaacaaa
    22921 gcctgcataa tgcccgccgc ctgtttgaaa gcggcgacat cgaccgtatc gaagtcggta
    22981 ccaccgcggg cctgcaacag attcaccgtt acctgttcgg cggcttatat gattttgcgg
    23041 gtcaaatcag ggaagacaac atttccaaag gcggttttcg ttttgccaac gccatgtatt
    23101 taaaagaggc tttggttaaa atcgagcaga tgcccgagcg gacttttgaa gaaatcatcg
    23161 ccaaatatgt tgaaatgaac attgcccatc cgtttttgga gggtaatggc agaagtaccc
    23221 gcatctggct ggatttggtg ctgaaaaaaa acctgaaaaa agtcgtgaac tggcaaaatg
    23281 taagtaaaac cctgtatttg caggcgatgg aacgcagccc cgtcaacgat ttagaactgc
    23341 gctttctgtt aaaggacaac ctgactgacg atgtggacaa ccgtgaaatc atctttaaag
    23401 gtatcgagca gtcgtattat tacgaagggt atgaaaaagg ctgaaggtcg tctgaaaagc
    23461 gatttcagat tgtttcagac gacctgattt ggtaggtgat cagacgggag cggatgagaa
    23521 aagaaattct gggtaagaat aatccggtct gaaatattgg aagaagaatg atggataaaa
    23581 atcagttaga acaagaattt cataaagcca tgttaaatat ttatcaggag gctttgaatt
    23641 tgccgcaacc ttacaaggcg acacgatttt tacaaattgt aaatgaattt ggtggtaaag
    23701 aggcggcgga taaattattg agtacggggg aaaagaagac tcagaccggt tttacagagc
    23761 tgattttgag tggtggcgga gtccacgcct tgaaatacag tatggaatat ctggtgttac
    23821 aaaagccgtg gtgtgattta tttactgaag agcaattagc tgtggcacgc aaacgattgg
    23881 agcgtgttgg atttgttttt ccgaagtaat tttgtacgaa acaaacatag atttttaaat
    23941 caatcggatt caatcaaatg aacgacatga ctttatatct catcattgcc ctcgtgcctt
    24001 tggcaggctc gctgattgcg ggtttgttcg gcaacaaaat tggtcgtgcc ggtgcgcata
    24061 cggttacgat actcggcgtg gcggtgtccg ccgtgctgtc ggcttatgtg ctgtggggct
    24121 ttatcgacgg cagccgcgcc aagtttgacg agaatgtcta tacctggctg acgatgggcg
    24181 gtttggattt ctccgtcggc ttcttggtcg atacgatgac ggcgatgatg atggtcgtgg
    24241 tcaccggcgt atcgctgatg gtgcatatct ataccatcgg ttatatgcac gatgagaaag
    24301 tcggctacca acgcttcttc agctacattt ctttgtttac tttcagtatg ttgatgctga
    24361 ttatgagcaa caacttcatc cagctcttct tcggctggga agctgtgggc ttggtgtcgt
    24421 atctcttgat cggtttctat ttcaaacgtc cgagcgcaac atttgccaac ctgaaagcct
    24481 ttttgatcaa ccgtgtcggc gacttcggct ttttgctcgg tatcggcttg gtgcttgctt
    24541 attttggcgg cagcctgcgc tatcaggacg tattcgctta tctgcccaac gtccaaaatg
    24601 ccaccatcca gctcttcccc ggtgtggaat ggtctttgat taccgtaacc tgtttgctct
    24661 tgtttgtggg tgcgatgggt aaatcggcac aattcccgct gcacgtctgg ctgcctgatt
    24721 cgatggaagg cccgaccccg atttccgcat tgattcacgc tgcgacgatg gttacggcgg
    24781 gtctgtttat ggtatcgcgt atgtcgccga tttacgaaat gagcagcact gcgctgtccg
    24841 ttatcatggt gatcggtgcg attaccgccc tgtttatggg cttcttgggc gtgattcaaa
    24901 acgacatcaa acgtgtggtt gcgtattcca ccctgtcgca actgggctat atgaccgtgg
    24961 ctttgggcgc gtctgcctat tccgtggcga tgttccatgt gatgacccac gccttcttta
    25021 aagccctgtt gttcttggcg gcaggcagcg cgattatcgg tatgcaccac gaccaagaca
    25081 tgcgccatat gggcaatctg aaaaaatata tgccggttac ttggctgacc atgctgatcg
    25141 gtaacttgtc gctgattggt acgccgttct tctccggctt ctactccaaa gattcgatta
    25201 tcgaagcggc gaaatacagc acactgccgg gcagcggctt tgcctatttt gccgtcctcg
    25261 ccagcgtgtt tgttaccgcg ttttacgcgt tccgccaata ctttatggtg ttccacggcg
    25321 aagagaaatg gcgcagcctg cccgaacacc attcagacgg ccacggcgaa gaacatcacg
    25381 gtttgggtaa aaacgacaat ccgcacgaaa gcccgttggt ggttaccctg cctttgattt
    25441 tgcttgccgt tccgtccgtc atcatcggct acatcgccat cgaacccatg ctttacggcg
    25501 atttcttcaa agacgtgatt tttgttaatg ccgatgcgca tccgaccatg cacattatga
    25561 aagaagagtt ccacggcgca ttggcgatgg tgtcgcacag cctgcattcg cccgttctct
    25621 atctcgctat cgcaggcgta ttgagcgcat ggcttttgta cgtcaaactg ccgcacctgc
    25681 cagcgaaaat tgcacagacg ttccgtccga tttacgtttt gtttgaaaac aaatactacc
    25741 tcgacgccct gtatttcaac gtcttcgcca aaggcacacg cgcattgggt accttcttct
    25801 ggaaagtcgg cgataccgcc attatcgaca acggtattgt caacggctct gccagactgg
    25861 tcggcgcggt tgccgcgcaa gtgcgtaaag tccaaacagg ctttatctac acctacgctg
    25921 ccgctatggt gttcggcgta ttggtcttgc tcggtatgac cttctgggga ttgttcagat
    25981 aaaaatgaga ttttcagacg acgtttaggt cgtctgaaaa aagaaacttg agaaacaatg
    26041 tcaaacacac aaaccatccg ctctgccgac ttcaccactt cccgcgcatg gggcgcactc
    26101 gacatcgcca atatgaacgg caccaccgtg cgcctgcact ggacggacca gccctacaaa
    26161 tggcacgtca acgacggcga ggaagtgttt gccagtcgat atgcactacc gagaaaacgg
    26221 cgaagaacac atcgttcggt tgaaaagcgg cgacatcttc tacgcaggaa tcggcgcgga
    26281 acacgtcgcc cacccgcgcg gcgaaacgcg gattttagtg attgagaaag aaggcagtgt
    26341 ttagggcgga tacgcataag tattatgtgc tttttcaggt atcctgaaaa tcataaatct
    26401 atcaacgaga ctttaatcat ggcaaatcta aatgtgagta gtaaaaaaat tattaatctg
    26461 ctaggtaatc aagatatgca ggaaaaatat tttatcattc ctgaatatca acgcccctat
    26521 cgctgggata ccgaaaaatg cgccgtatta tggtctgata ttgtggattt tcgccagtcg
    26581 tccacaggaa aaaatgaaga atattttctt ggtacaatcg ttgtttgccc caatgaaaaa
    26641 ggtaatttgg aagtaattga tgggcaacag cgtttaacat cattactgct gttattgcgt
    26701 gcgttttatc gagttttaga gcaaacggaa agtacccccg ataccgcagc caaaattacc
    26761 acacttaaaa gcaaaattgc cccctgtatt tgggatgttg atgttctaac ggctgaaatt
    26821 accgatagaa ccgcaatccg gctgcatacg caagttgcca cagaaacaga taacgaaatt
    26881 ttgcacgaga ttttggcaaa cggtgaaatc tcagaagagt gtcgttctaa ttacgccgtt
    26941 aattatttgt atttcctgaa acagtgccaa gaatacgcca gaaccaaccc aatggattgg
    27001 tatcatcttt gtttaactat tttgaatgac tgtattttac ttccgattga aagtgacagt
    27061 ttaaatagtg cattaaccat attttccaca ttaaacgata ggggcttgcc tttgtcggat
    27121 gcagatattt ttaaagccag gctttataag atggcgcagc agaaagataa atttgttgaa
    27181 caatggaagg aacttgttgc gttggcagag ggtgcatcta tttccgtaga tgatttgttc
    27241 cgctattaca cccatgtatt aagggcgaaa gaaaagatta cggataaaga aattgggctt
    27301 cgccgttttt atactgacaa ccgaaacaaa cagttggaaa acgaaaattt gttcaatcac
    27361 ttaattgagc tggctggatt ttggcaggat attaacaaca gcaaaactaa ccaagacgtg
    27421 gaaacttcga actttgtaaa tgacgaggcg gccaagtggc tgcaatgtct gaatgtctat
    27481 cctaatgagt tttggaaata cgccgcgagt gtgttcttca ttgcaaacaa gggtgcgcaa
    27541 tcctttaaag atgattttgc agcattatta aaacagctta ctgcattttt gtatgctaaa
    27601 tttttagttg cgccaaccgt aactgcaatt aaagatgata tttataaatt atgtgttgcg
    27661 atttatcacg gtgaaaaaaa cgctttatca tatgttatgc ctgcacccat acactatgaa
    27721 caaaatacag gacgtataac taaagggttg ttaatgatta atgcttatct atttgatgaa
    27781 aagcagaaat tattgccaac taaaactgaa atcgaacata tctttccgaa aaaatggcag
    27841 gaaactaact ataatggttg gtcaatcata gatgcggaac aatatttaga acaactaggt
    27901 aataaaattg tttttgaaaa acggttaaat attcaggcag gtaacggcta ttttggtaag
    27961 aaaaaggagc aatatcaaaa atccgatatt gttgaagtgc gtgaattagc aggtgaaaat
    28021 gccaatgatt ggttaccgag tgatattcag aaacgccagc agcagattgt ggatagattg
    28081 aaacagtttt tcgataattg ttttgaagca gccagcatcc ccaaaaaaaa ttaacttttc
    28141 caataacctc caaaccaaac aggtaacgca atgttttcta accatcttct cagcttggca
    28201 atatggatac ccattgccgc aggtgtgctg gttttggcga ccggtaaaga cagccgtgcg
    28261 ccgctggcgc gtgtgcttgc ctttatgggt gcgcttgccg gtttcttggt aacgctgccc
    28321 ctgtttaccg gtttcgaccg tttgagcggc ggctatcaat ttaccgagtt ccacgagtgg
    28381 attccgctgc tgaaaatcaa ctatgcattg ggcgtggacg gtatttcagt gctctttatc
    28441 atcttgaatg cgtttattac gctgttggtg gtgctggcgg gttgggaagt tattcagaaa
    28501 cgcccggcgc agtatatggc ggcattcctg atgatgtcgg gtttgattaa cggtgcgttt
    28561 gccgcgcagg atgcgattct gttttatgtg ttcttcgagg gtatgctgat tccgctgtac
    28621 ctgattatcg gtgtatgggg tggcccgcgc cgcgtttatg cgtcggtcaa gctgttcctc
    28681 tatacgctga tgggttcgct tttgatgctg gttgccatgg tttacctgta ctatcagaca
    28741 ggcagcttct ctattgtcga tttccaaaac atcaagcaga ttccattggg cgtacaacag
    28801 cttttgtttg tggcattctt cctgtcattt gccgtaaaag tgccgatgtt ccctgtgcac
    28861 acttggttgc cggatgccca cgttgaagcg ccgaccggcg gttcgatggt gttggcggca
    28921 attacgctga aactgggtgc gtatggtttc ttgcgtttta tcctgccgat tatgccggat
    28981 gcggcacgct attttgcccc tgttatcatc gtattgagcc tgattgccgt gatttatatc
    29041 ggtatggtgg ctttggtgca aaccgatatg aaaaaactgg tggcgtattc gtctatcagc
    29101 catatgggtt ttgtaacgct tgggatgttt ttgtttgttg acgggcagtt ggacgactgg
    29161 gcgttgaaag gcgcaatcat tcaaatgatt tcgcacggtt tcgtgtcggc ggcgatgttt
    29221 atgtgtatcg gcgtgatgta cgaccgcctg catacccgca atattgctga ctatggcggc
    29281 gtggtcaatg tgatgcctaa gtttgcggcg tttatgatgt tgttcggtat ggccaatgcc
    29341 ggtctgcctg caacttccgg cttcgtgggc gagtttatgg tgattatggg cgcggttaaa
    29401 gtgaatttct gggtgggcgc gttggcggcc atgaccctga tttacggtgc gtcttatacc
    29461 ctgtggatgt acaagcgcgt gattttcggc gcgatccaca atccgcacgt tgccgaaatg
    29521 aaagacatca actgccgcga atttgcgatt ctggcgattt tggcggttgt ggtattgggt
    29581 atgggtctgt atccgaacgc atttatcgaa gtggtgcatc aggcggcaaa cgatttgatt
    29641 gcccatgtgg cacaaagcaa gatttgaggt gtgtaaatga actggtctga tttgaattta
    29701 atgcccgcac tgcccgaaat cgcgctgctg tcgctgctgg tgttattgtt gctggcagac
    29761 ttgtgggtca gtgatgacaa acgccgttgg acgcattacg gcgcgttggc aacggtggcg
    29821 gttacggctg tggtgcagct tgccgtttgg gaacagggca gcacgtcttc gttcaacggc
    29881 atgtatattg cagacggtat gtcgcgtttg gcaaaaatgg ttttatatgc cttgacgttt
    29941 gccctgtttg tttatgccaa gccctacaac caagtgcgcg gtattttcaa aggcgagttt
    30001 tacaccctgt cgctgtttgc cctcttgggc atgagcgtga tggtgagcac ggggcatttt
    30061 ttaactgcct atatcggttt ggaactcttg tcgcttgccc tttacgccct gattgccctg
    30121 cgccgcgatt ccggctttgc cgccgaagcc gccttgaaat attttgtttt gggcgcgctg
    30181 gcatccggcc tgctgctcta cggtatttct atggtttacg gcgcaaccgg ttcgctggaa
    30241 tttgccggcg tgctcgcctc ttccttcaat gaagaagcca acgaatggct gttgaaactg
    30301 ggtctggtgt ttatcgtcgt cgccgtcgcg ttcaaactcg gtgcggtgcc gttccatatg
    30361 tgggtgcccg acgtgtatca cggcgcgccc acttctgtta ccgccctggt cggcactgcc
    30421 ccgaaagtcg ccgccgtcgt tttcgctttc cgcatcctcg ttaccgggct gggaaccgtg
    30481 catcatgact ggtctctgat gtttgccctg cttgccgccg cctcgctgct ggtcggcaac
    30541 cttgccgcca tcatgcagac caatatcaaa cgtatgctcg cctattccac cgtatcgcat
    30601 atggggttta tcctgctggc gtttatggcg ggcgcggtcg gctttgcggc ggggctgtat
    30661 tacgccatta cctacgcgct gatggcggcg gcagggttcg gcgtgttgat ggtgttgtcg
    30721 gacggggaca acgagtgcga aaacatcagc gatttggcag ggttgaacca acaccgcgta
    30781 tggcttgcct ttttgatgct gctggttatg ttctctatgg cgggcattcc accgctgatg
    30841 ggtttttacg ccaaattcgg cgtgattatg gcactcttga aacaaggcca tgtttggttg
    30901 tctgtatttg ccgtcattat gtcgctgatt ggtgcgttct actatctgcg cgtggtcaaa
    30961 gtcatgtatt ttgacgaatc cggccgcgcc cgtcccaccg tcggcggcaa caatgccgca
    31021 aaattccttt tgagcgtcaa cgcgctgctg ttggtgctgt ggggcattat gccgcaaacc
    31081 gttatcgact ggtgcgccaa ggcgttggaa aacacgctgt aagccgccgc aatggcagcc
    31141 gtgtcagagg ctgccgtttt tgttaagata tgccgttccg caacgcagtt cagacggcat
    31201 cgccgccgac aacgccaaaa cagaaagccc accatgaccg catccatgta catccttttg
    31261 gtcttggcac tcatctttgc caacgccccc ttcctcacga ccagactgtt cggcgtggcc
    31321 gcgctcaagc gcaaacattt cggacaccac atgatcgagc tggcggcagg tttcgcgctg
    31381 accgccgttc ttgcctacat cctcgaatcc cgtgcgggag cggtacacga tcagggttgg
    31441 gagttttacg ccaccgtcgt ctgcctgtac ctgatttttg cgtttccctg tttcgtgtgg
    31501 cggtattttt ggcacacgcg caacagggaa taggcaggca taggaatgcc gtctgaaacc
    31561 ctttcagacg gcatttgttt cattcaagtg caggccggca tcgctgtgcc ggcacgtttc
    31621 agccggcgat atacgccggt tttaatattt gcgggcgact gcaaattctg ccaactgccg
    31681 caggcgcagg gctttgtcgc cgaagggttc gagcagcgcg accgcttcgg caaccagttt
    31741 gtgtgcgtat gagcgcgccg cttccaagcc catcagtttc acataagtcg gcttgtcgtt
    31801 gtctgcgtct ttgcccgccg ttttgcccaa agtcgccgtg tccgcttcac aatccaacac
    31861 atcgtcaatg acttggaacg ccagccccag ttttgccgca taatcgtcca ataccgccag
    31921 actgtcttca gacagattcg ggcaggacat cgcccccaat aaaaccgccg cacggattag
    31981 cgcacccgtt ttcaggctgt gcatctgttc caaatcggct tgaaccattt gtttgccgac
    32041 attcgccaaa tcgattgcct gaccgcccgc catgcccgcg ctgccgcccg ctttcgccaa
    32101 caccgacaac attgccaact ggcgtgctgc gggcagttct gtcggacggc ttaacacgtc
    32161 gaaggcttgg gtttgcaaag cgtcgccgac cagtagcgcg gtggcttcgt cgtattgtat
    32221 atggcaagtc ggtttgccgc gccgcaggct gtcgttgtcc atcgccggca tatcgtcgtg
    32281 aaccaaagaa tagacgtgga tcatttcgat tgccgccatt gcctgttcta ctgcttcatg
    32341 cacggcttcg cctaattccg aagctgctag aaccagcatc ggccgcagac gcttgccgcc
    32401 gtccaaagcc gcatagcgca tcgcttcgtg cagcgtgtgc gggatttcgt tttcagacgg
    32461 caaaaagcgt tccagcagca gctctgtttg cgcctgcgcc ctctgttgcc acgttttcaa
    32521 atcattcgtc ggattcaagg ttcaactcct tcagcccgtc cgcatccaaa acctgtaatt
    32581 tctgttcgac ttgcgccagc ttggtttggc agtacctgac cagctcgttg ccttcctgat
    32641 aggcggcaag cgcgtcttcc aagggcattt cgccctgcat agactgcgtc agcgattcga
    32701 ggcgcgacaa ggcttcttca aacgatttcg gggcgttttt cttcatcgta tttccttttc
    32761 ggttgaaacc ccgccacttg gacatctgtc cttcggggcg gcaggatcag actttatttg
    32821 ggaggggtgt aaccctttcc aaatcagggc aatacatagg gcggtgcttt atgtgccgtc
    32881 ctgtgtgttg gaacatagtt tcggatgttc cggtaaaaag cggattgtag catttttgaa
    32941 aaacggatgc cgtctgaaac ccgaatccgg cttcagacgg cattttttcc gcccaggcgg
    33001 caaggcgtta cccgggcagt tcgtcggtga tgccctgcaa aaaggcgagg cgttcggggc
    33061 ttgccgcccc ggtttcggcg gcggctttga aggcgcagct gggttcggcg cggtgggtgc
    33121 agttgtggaa gcggcattgc ccgacaaggt ggcggaaatc ggggaaatag cgcggcaaat
    33181 cggcggcttg gaggtggtgt aaaccaaatt cttgcaaacc cggggagtcg atgagttggg
    33241 tttcgccgtt caaatcataa agccgggcgt gggtggtggt gtgttttccc gagtcgagtg
    33301 cggcggaaat gtcgccggtg cgggcggttt ggctgcccaa aagggcgttg gtcagggtgg
    33361 atttgcccat accgctctgc ccgagcagga tgttgctgtg cccttgcagg gcggggcgca
    33421 ggctgccggc gttttccagt gcgcgggttt cgatgacggg ataacccagc gtttcgtaga
    33481 atttgagttt ttcgcgccaa agggcggttt cgggcaggtc ggctttgttc aggacgatga
    33541 cggcgcggat gccggcggct tcggcggcaa gcagggcgcg ttgcagcagc cgcacgctcg
    33601 gactcgggac ggcggcggtt acgatgagga gttgggtaac gttggcggcg atgagtttgg
    33661 ttttccacgc gtcttggcgg tagagcaggc tttggcgcgg taaaaaatct tcaatcacaa
    33721 cttgttcggc gttgacgggg ctgatgcgga cgcggtcgcc gcaggcgaaa tcgacgcgtt
    33781 ttttgcgggt gctggcttcg taggttgtgc cgtcgggcgt gcggacaatg tagcggcggc
    33841 cgtagctggc ggtaatttgg gcggtgtcgt tcatggtttc tttggggttg ggtgtggaaa
    33901 tgccgtctga aaacgggtgt tcggacggca tcggttcagt cgtgctgcca ctcgacgtgt
    33961 tcgttgagga agccgccgct ctggtgcgcc cagagtttgg cgtaaagccc gcgtttttcg
    34021 aggagttcgg cgtgtgtgcc ttcttcgatg atgcggcctt tgtcgaggac gacgagcctg
    34081 tccattgcgg cgatggtgga gaggcggtgg gcgatggcga tgacggtttt gccgtccatc
    34141 attttgtcga ggctttcctg gatggcggct tcgacttcgg aatcgagcgc gctggtggct
    34201 tcgtcgagca gcaggatggg cgcgtctttg agcatcacgc gggcaatggc gatgcgctgg
    34261 cgttgcccgc cggagagttt cacgccgcgt tcgccgacgt gcgcgtcata gccgcgccgc
    34321 cctttggcat cggaaagttc ggggatgaag ccggcggctt cggcgcgttc ggcggcggaa
    34381 accatttcgg catcggtcgc gtcggggcgg ccgtaaataa tgttgtcgcg cacggaacgg
    34441 tgcagcagcg aggtatcttg cgtgaccaaa ccgatttggg cgcgtaaaga ttcttgggta
    34501 acgccgctta tgtcctgccc gtcgatcgaa accgtgccgc tttgcggttc gtagaagcgc
    34561 aaaagcaggt tgacgatggt ggatttgccc gcgccgctgc gtccgatcaa gccgactttt
    34621 tcgcccgggc ggatggtgag gttgaagccg ttgagcagcg gtttgccggc ttcgtagcag
    34681 aaatcgatgt gttcaaattt gattgcgcct tgcggcacgt tcagcggcag tgcccggggc
    34741 ttgtcgagga tggtgtgcgg tttggacagg gttgccatgc cgtcgccgac ggtgccgatg
    34801 ttttcaaaca gccgcgcgga ttcccacata atgtattgcg acaaaccgtt gacgcgcaac
    34861 gccatcgcgg tggcggtggc gaccgcgccc acgccgacct gcccgttgtg ccagagccag
    34921 atgcccagtg cggcggtgga gagggtcagg gaggtgttga cgatgaagct gcacgaatgc
    34981 agcagcgtcg ccagccgcat ttgggcgcgc accgtaacca taaattcttc catcgactgc
    35041 ttggcatagg cggcttcgcg cgcgccgtgg gagaagagtt tgacggtggc gatattggaa
    35101 taggcatcgg taatgcggcc ggtcatcagc gagcgggcat ccgcctgcca tgcggcggtt
    35161 tgccccaatt tgggaatcag caggcgcatc accgaagcga aaccgacaat ccagccgata
    35221 aagggcagca gcagccatga gtcgagcgag gcgagaatca cgccggaggt aatgaaatac
    35281 accgacacat aaacgaccat atcggcaacc gtcatcaccg cgtcgcgcaa cgccagcgcg
    35341 gtctgcatga ctttggcgga cacgcgtccg gcaaattcgt cctgataaaa accgaggctt
    35401 tggttcagca tcaggcggtg gaagttccag cgcaggcgca tggggaacac gccctgaagg
    35461 gtttgcaggc gcacgttgga cgcggcatac gcccacacga ccgaaaacac catcatcgcc
    35521 gccattgccg ccagttccca acctttttcg gcaaacagtt cggcgggcgc gtatttgccg
    35581 agccactcca cgattttgcc cataaattga aaaaccaggg cttccataat gccgatgccg
    35641 gcggtcagcg cagccagggc ggctatccat ttccgcacgc cggccatgct gctccagaca
    35701 aaccgccaca agcctttttc tggcgttttc ggggcggctt cgggataagg gtcgattcgg
    35761 gactcgaacc aggaaaagat tttgttcaac attgttttcg atttcggtaa aacagtttca
    35821 gacggcatca aacacaatgc cgtctgaaag gaaggacaat aacgccattt tacgggaaaa
    35881 gccgtcggga agacagcgcg aggcggaaac gcagggtttc gtcagggcaa acgtcgcgcc
    35941 gccttcagac ggcattattt cagcaggttt ttcaaagcaa ggcgcacgcc ttcgcccacg
    36001 tccgtcccct ccggaacgcc tttgaccgcc gcttttgctt cgcgttcgct gtaacccagc
    36061 gcaagcagcg tgctgacgat gtcttccgtt tcgtcggcgg cgggtgcggc ggcaaacagc
    36121 ccgtccgtta ccgtatgcgc gaccagcttg ccgcgcagtt ccaagaccat acgttcggcg
    36181 gtttttttgc cgattcccgg cgcggaagac aggcgtttca cgtcttcttc tgcaaccgcc
    36241 tgcgccagct cgtctgccgt catcgccgac aaaatgccca aagccgtttt cgcgccgatg
    36301 ccgccgacct tgatcagttg gcggaatgtt ttgcgttctt ccgcagtggc aaaaccaaat
    36361 aaaagatgtg cgtcttcccg aatgataagc tgggtaaaca gttgtacgct ttcacccacg
    36421 ggcggcaggt tgtagaaggt ctgcatcgat acgtcggcct catagccgac accgttgaca
    36481 tcgatgacga tttgcggagg gtttttttca accagtttgc cggtcagtct gctgatcatg
    36541 tatgccgaat cctgaagtgt cgggtgcaaa atgccgtctg aaaccggttc gggcttcaga
    36601 cggcacggat tgtatcaaat tcagtcgtcg cggcgggagg aaatcacgcg gccggtacgg
    36661 gcatcgacaa cgactttgta ttcctgtccg tttttgacga tttcgacatc atagtgcgga
    36721 cggccgttgt cgtgttcgag atcgatgtcg gtgattttgc cgccgacacg cgccaacgct
    36781 gctttttcgg cttgggcgcg gctgatgatt ttgtcttgtt tgctgtgttg gtgtgcggcg
    36841 tgtccgtggt cgtcatcgcc gtgtccgtcg tggtgggcga gtgcgggggc ggaaatgccc
    36901 atcagtgcgg ttgcggcggc ggtcaagaga aggcgtttga agttcatgtt ttgctcctgc
    36961 gaagtatctg gtggtgtttg aaagacgtaa aggcgggaca tcaaccggca gtcaataccg
    37021 cccgaaccat accgcgcgcc tgagcttcgg cttcggcggc gcgttcctgc gaggcaaacg
    37081 gtcccatttt gacgacgtat tcgtaacggc gtttttcaat ggcgaggttc gggcttgatg
    37141 ccgaagcggc gaagttttgg gcggcttggt tcagataggc ttgtgcttcg tgttccgtac
    37201 cgaaagattt caagtcgatg aagatgtctt tgttttcggc aaccggtgcg gattggcccg
    37261 ggacgatttg ttcgattttg acgtgcgccg tcccttggtt gacaaagccc aatttttgcg
    37321 cggcggcttt ggatacgtcg atgatgcggt tgccgtggaa ggggccgcgg tcgttgacgc
    37381 ggacgatgac gcttttgccg tttttggtat tggttacgcg cacatagctg gggatgggca
    37441 gggttttgtg ggcggcggta aaggcgttca tatcgtatcg ttctccgccg gaagttttgc
    37501 gcccgtgaaa cctgccgccg taccacgagg cgttgccggt ttgcgtgaat tcggcgactt
    37561 ggtttttcgg cgtgtagcgt tttccggcga ctttgtagct gcggttggcg gaggcgtgca
    37621 gtttttctgc cttgaccact gcgtcggcgg atgccgtctg aagggagtgt gtgccgaatg
    37681 cggcggtgag aaggaaaagg gtttttcggg ttaaagtcaa aacgtgttcc gttcttgagt
    37741 tgaagacgaa tgggcatcat gcccgccgga tacgttccga accgccgtac agtgcggacg
    37801 gcggttcgga atgtgtccgg ataggttttc agacggcatg aacttgcgtt caaacgccgc
    37861 ctgcgtaacc gtgttgccgc cacgcttcaa agagaatcac ggcaacggta ttggaaaggt
    37921 tcatactccg actgccgggc tgcatcggca ggcggatttt ttgcgcggcg ggcaggctgt
    37981 cgaggatgtc ggcaggcagt ccgcgcgttt ccggcccgaa cagtaaaacg tcgccttttt
    38041 gaaacgcggt ttcatcgggg cgcgccgtgc ctttggtggt cagggcgaaa atgcgcctgc
    38101 ctgcgagtgc cttgaggcag tcgtcgaagt tttcgtgcac cgtcaggctg gcgaactcgt
    38161 ggtagtcgag cccggcgcgt ttcattttgg cggaatccaa tgggaagccg agcggtttga
    38221 caaggtgcaa atccgcgccg gtattggcgc acaggcggat gatgttgccc gtgttcggcg
    38281 ggatttccgg ctggtataaa acgatggtaa acataaatat caatcactta taggcgcgta
    38341 accttgccac aaggcggatg gggtgtcaaa aaatttagtt attttttcat tggcgtgcgt
    38401 gccagcgtcc agcagcagat gcggttcgcg cccgattttt tcagcgtctt tgccagttcg
    38461 tccagcgtcg cgccggtggt aaagacatcg tcgattaaca gaatattaca gttttccggt
    38521 atcggtgtgc ggatttcaaa ggcgtttttg atgtttcgcc gccgttcgct gcctttgagc
    38581 gtgctttgcg gcgggcggtg gtgtcggaat acggtgtgtc ggggcagtat ctgccagccg
    38641 tagcgttgtg ccaacaaccc gacgatgctt tcactttggt tgaacccgcg ttgcagcagc
    38701 cgctccctgc ttagcggtac gggcaggacg aaatcgaaac attcgtctgc aagccggtcg
    38761 ggcgggtttt gtaccatcag gtttgccaat gggcgaacca tgcccaaatc tgccaagtgt
    38821 ttcagcgtac gtatcatgtt gctgacgggc ggttcgtaat gcagcgaagc ccacatccgg
    38881 tcgaatgcgg gcggtttttt ctgacagccg ccgcacaccg cgccgccttg aacgtgcctg
    38941 aaacacaggg ggcagctgtt tgccgcgtcg gtgcggtatg ccgccaaatc cgccgcgcag
    39001 ccggcgcaga tgccgtctga aacgccggac gaaccgtggc ataatacgca acgcctgata
    39061 gtgggcgcgt ctgcgatgcg ccgccaacaa gagagaaaat tcatgcctga tgctgccaaa
    39121 aaagtttacc tgatacacgg gtggggggcg aaccgccacg tgttcgacga cctgatgccc
    39181 cgtctgcctg caacgtggcc ggtgtccgcc gtcgatttgc ccggacacgg cgacgcgccc
    39241 ttcgcgcaac ccttcgatat tgaggcagcc gccgacgctg ttgccgcgca aatcgatacg
    39301 cccgccgaca ttctcggctg gtcgctcggc ggattggtcg cgctgtatct ggcggcgcgc
    39361 catcccggaa aagtccgttc gctctgcctg acggcgagtt tcgcccggct gacggctgac
    39421 gaagactatc ccgaagggct tgccgcgcct gcattgggca aaatggtcgg tgcgttccgt
    39481 tcggattatg ccaaacatat caaacagttt ctacaattac agcttctgca cacgcctgat
    39541 gcggacggaa tcataggcag aatcctgccc gatttggcac ggtgcggcac gccctccgcc
    39601 ctgcaggagg cgttggacgc ggcggaaagg gcggatgcgc ggcatttgtt ggacaagata
    39661 gatgttccgg tgctgctggt gttcggtagc aaagacgcga ttacgccgcc gcgtatgggc
    39721 gagtatctgc accggcattt gaaaggaagc aggctggttg tgatggaaaa ggcggcacac
    39781 gcgccgtttt tgagccacgc ggaagcgttt gccgcgctgt accgcgactt tgtcgaaggg
    39841 gtttgagatg aatccgcagg acaaatgctg gcaggttcac cgccatctcg ccgaacatgc
    39901 cgaccaacgc ctgacactcg tccgcaacgc gcccaaacat atcctgcttg ccggggcgga
    39961 tgcggacatc agccgcagcc tgctggcgaa acgctatccg caggcggtat ttgaagaata
    40021 cgattcccgt gcggattttt tggcggcggc ggcagccgcg cgcaaaggcg gtttttggca
    40081 aaggtttgcg ggtaagggcg tggtgcagca ctgccaatcc ccgaccgcgc cgctgcccga
    40141 agcgtgtgcc gatatgttgt ggtcgaatct cggactgttg gcggcggaac aaatccttcc
    40201 tgtgctgcac aactgggcgc gcgcgctgga gacggacggg ctgctgtttt ttacctgctt
    40261 cgggcgcgac acttttaccg aactgaaatg ccgtctgaaa gaaaacggca ttgaaagccg
    40321 cagcgtgatg tttcccgata tgcacgactt gggcgatatg cttgctgaaa acgggtttta
    40381 cgaccccgtt accgatacgg cgaagctggt gttggattac aaaaaggcgg aaacgttttg
    40441 ggcggatatg gacacgctgg gcgtttggcg ggcgatgtcg tggaacgatg aaaacgccgc
    40501 gcgttcgtgt gtcgggacaa tatttgaacg ggaaggcggt ttgggcatta cgctggaaac
    40561 ggtgtacgga cacgccgtga aaaaactgat gctgccgcaa ggggagaacg tggtgcagtt
    40621 ttttccgaag agatgatgtg cagatgccgt ctgaagccgt gtccaagttt cagacggcat
    40681 ctgtctgtga aaaccgatag aaataaagga aataccgatg tatagtg
//
LOCUS       DAWFJW010000003        40333 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFJW010000003 DAWFJW010000000
VERSION     DAWFJW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.003"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(143..233)
                     /locus_tag="WGR19_000098"
     tRNA            complement(143..233)
                     /locus_tag="WGR19_000098"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ser,seq:gga)
     gene            complement(269..355)
                     /locus_tag="WGR19_000099"
     tRNA            complement(269..355)
                     /locus_tag="WGR19_000099"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Leu,seq:cag)
     gene            complement(391..477)
                     /locus_tag="WGR19_000100"
     tRNA            complement(391..477)
                     /locus_tag="WGR19_000100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(441..443),aa:Leu,seq:cag)
     gene            566..2941
                     /gene="rnr"
                     /locus_tag="WGR19_000101"
     CDS             566..2941
                     /gene="rnr"
                     /locus_tag="WGR19_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0797708.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQF
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3280..4743)
                     /gene="guaB"
                     /locus_tag="WGR19_000102"
     CDS             complement(3280..4743)
                     /gene="guaB"
                     /locus_tag="WGR19_000102"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0797709.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4890..7448)
                     /gene="glnD"
                     /locus_tag="WGR19_000103"
     CDS             complement(4890..7448)
                     /gene="glnD"
                     /locus_tag="WGR19_000103"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0797710.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKAKRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYQGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(7497..7817)
                     /locus_tag="WGR19_000104"
     CDS             complement(7497..7817)
                     /locus_tag="WGR19_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0797711.1"
                     /translation="MMGNKLTLPAELPAEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8141..8614)
                     /gene="bfr"
                     /locus_tag="WGR19_000105"
     CDS             complement(8141..8614)
                     /gene="bfr"
                     /locus_tag="WGR19_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0797712.1"
                     /translation="MKGDRLVIRELNKNLGLLLITINQYFLHARILKNWGFKELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8642..9106)
                     /gene="bfr"
                     /locus_tag="WGR19_000106"
     CDS             complement(8642..9106)
                     /gene="bfr"
                     /locus_tag="WGR19_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0797713.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMVRAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9570..10553)
                     /gene="lipA"
                     /locus_tag="WGR19_000107"
     CDS             complement(9570..10553)
                     /gene="lipA"
                     /locus_tag="WGR19_000107"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0797714.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYKKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10546..11169)
                     /gene="lipB"
                     /locus_tag="WGR19_000108"
     CDS             complement(10546..11169)
                     /gene="lipB"
                     /locus_tag="WGR19_000108"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0797715.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11171..11446)
                     /locus_tag="WGR19_000109"
     CDS             complement(11171..11446)
                     /locus_tag="WGR19_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0797716.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11631..12701
                     /locus_tag="WGR19_000110"
     CDS             11631..12701
                     /locus_tag="WGR19_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0797717.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYMEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12897..13844)
                     /locus_tag="WGR19_000111"
     CDS             complement(12897..13844)
                     /locus_tag="WGR19_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0797718.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13859..14266)
                     /locus_tag="WGR19_000112"
     CDS             complement(13859..14266)
                     /locus_tag="WGR19_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0797719.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHVGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            14347..15006
                     /gene="ung"
                     /locus_tag="WGR19_000113"
     CDS             14347..15006
                     /gene="ung"
                     /locus_tag="WGR19_000113"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0797720.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            15108..15755
                     /locus_tag="WGR19_000114"
     CDS             15108..15755
                     /locus_tag="WGR19_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0797721.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            15828..16301
                     /locus_tag="WGR19_000115"
     CDS             15828..16301
                     /locus_tag="WGR19_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ0797722.1"
                     /translation="MKTTGLRPSILPLPIRLVPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            16649..17593
                     /locus_tag="WGR19_000116"
     CDS             16649..17593
                     /locus_tag="WGR19_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0797723.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            17735..17869
                     /locus_tag="WGR19_000117"
     CDS             17735..17869
                     /locus_tag="WGR19_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0797724.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(18159..18674)
                     /locus_tag="WGR19_000118"
     CDS             complement(18159..18674)
                     /locus_tag="WGR19_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797725.1"
                     /translation="MKSKLPFILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(19140..21059)
                     /locus_tag="WGR19_000119"
     CDS             complement(19140..21059)
                     /locus_tag="WGR19_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0797726.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKFTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPKVREQEIRNFLGGFNFAGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(21136..21531)
                     /locus_tag="WGR19_000120"
     CDS             complement(21136..21531)
                     /locus_tag="WGR19_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ0797727.1"
                     /translation="MMRRRGKKMLFEENPIDGHFTEYGHRSGEIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            21704..23011
                     /locus_tag="WGR19_000121"
     CDS             21704..23011
                     /locus_tag="WGR19_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0797728.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAAEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFSLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDNRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            23004..23438
                     /locus_tag="WGR19_000122"
     CDS             23004..23438
                     /locus_tag="WGR19_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797729.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(23497..23766)
                     /locus_tag="WGR19_000123"
     CDS             complement(23497..23766)
                     /locus_tag="WGR19_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0797730.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(23948..26398)
                     /gene="lon"
                     /locus_tag="WGR19_000124"
     CDS             complement(23948..26398)
                     /gene="lon"
                     /locus_tag="WGR19_000124"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0797731.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            26483..26674
                     /locus_tag="WGR19_000125"
     CDS             26483..26674
                     /locus_tag="WGR19_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ0797732.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNRSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            26649..27752
                     /locus_tag="WGR19_000126"
     CDS             26649..27752
                     /locus_tag="WGR19_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0797733.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNLDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(27824..29569)
                     /gene="recD"
                     /locus_tag="WGR19_000127"
     CDS             complement(27824..29569)
                     /gene="recD"
                     /locus_tag="WGR19_000127"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0797734.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDATGARQNLAKWFQGAGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(29637..30332)
                     /gene="lolD"
                     /locus_tag="WGR19_000128"
     CDS             complement(29637..30332)
                     /gene="lolD"
                     /locus_tag="WGR19_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0797735.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            complement(30325..31572)
                     /locus_tag="WGR19_000129"
     CDS             complement(30325..31572)
                     /locus_tag="WGR19_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0797736.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            31777..32055
                     /locus_tag="WGR19_000130"
     CDS             31777..32055
                     /locus_tag="WGR19_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797737.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(32111..32728)
                     /gene="recR"
                     /locus_tag="WGR19_000131"
     CDS             complement(32111..32728)
                     /gene="recR"
                     /locus_tag="WGR19_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0797738.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKLAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(32795..34333)
                     /locus_tag="WGR19_000132"
     CDS             complement(32795..34333)
                     /locus_tag="WGR19_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0797739.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAMQNEQAGGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIHDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNVYEERVARLPANEAKPSFEQEKAAIENELKMKKAVADFN
                     KAKEKLGDDAFNHPASLAEAAKNSSLKVETQETWLSRQDAQMSGLPENLINAIFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     EVLAQLSGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAITPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(34393..34767)
                     /locus_tag="WGR19_000133"
     CDS             complement(34393..34767)
                     /locus_tag="WGR19_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0797740.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFKRQAPCRQG"
     gene            34917..36545
                     /locus_tag="WGR19_000134"
     CDS             34917..36545
                     /locus_tag="WGR19_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0797741.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            36617..37870
                     /locus_tag="WGR19_000135"
     CDS             36617..37870
                     /locus_tag="WGR19_000135"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0797742.1"
                     /translation="MQTYLVGGAVRDYLLGLPIKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECWAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            38143..38451
                     /locus_tag="WGR19_000136"
     CDS             38143..38451
                     /locus_tag="WGR19_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797743.1"
                     /translation="MTLKTDLLLKINNEDYQRLILKHISEFSEGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            38533..39564
                     /gene="ruvB"
                     /locus_tag="WGR19_000137"
     CDS             38533..39564
                     /gene="ruvB"
                     /locus_tag="WGR19_000137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0797744.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(39587..40315)
                     /gene="rpe"
                     /locus_tag="WGR19_000138"
     CDS             complement(39587..40315)
                     /gene="rpe"
                     /locus_tag="WGR19_000138"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0797745.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
BASE COUNT         9140 a        10572 c        10833 g         9788 t
ORIGIN      
        1 ataccgcaaa acataccaca aaaaacggta acggatagtt gtaagcggtt gatgacgatt
       61 atagacaata acgggttttc ccaatgaaat tattgtttga ataataaaaa atcccaaacc
      121 gtaaaagttt gggatttgta tttggcagag aggaagggat tcgaaccctc gatacgctat
      181 tcacgtatac acgctttcca ggcgtgcgac ttaaaccact catccacctc tctaatggcg
      241 gaaattatcc caatcggaat aatttatttg gtgcccggga gaagactcga acttccacac
      301 ccgtgaggat accagcacct gaagctggcg cgtctaccaa ttccgccacc cgggcaatct
      361 aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg gagaagactc gaacttccac
      421 acccgtgagg atactagcac ctgaagctag cgcgtctacc aattccgcca cccgggcttt
      481 gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa gaagtcgcta ttatatagtt
      541 cataaagaga atgtcaacag tccaaatgaa taaaaatatt aaatctttaa atttacggga
      601 aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa catcctttgc ccagtcggga
      661 atggataatc gaattgttgg agcgcaaagg tgtgccttca aaaatcgaat cgcttgcgcg
      721 cgagctgtcg attacggaag acgagtatgt ctttttcgaa cgccgtctga aggcgatggc
      781 gcgggacggt caggttttaa tcaaccgccg gggcgcggtt tgcgcggcgg acaaattgga
      841 tttggtcaaa tgccgtgtcg aggcgcacaa agacggtttc ggttttgccg tgccgctcac
      901 gcccgccaaa gacggtgatt ttgttttgta tgaacgccag atgcgcggca ttatgcacgg
      961 cgatattgtc actgttcgtc ctgccggcat ggaccgtagg ggccgccgcg aagggacagt
     1021 tctggatatt gtcgaacgcg cgcaaagcaa agtggtcggc cgtttctata tggatagggg
     1081 cgtggcgatt ttggagccgg aagacaagcg tctgaaccaa agcatcgtat tggaaccgga
     1141 cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc gtcggcgaaa ttgaggttta
     1201 tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa gttttgggcg attatgccga
     1261 cagcggcatg gagattgaaa ttgccgtgcg caagcatcat ttgccgcacc aattcagtga
     1321 agcgtgtgcc aaagccgtga aaaaaattcc cgaccatgta cgcaaaagcg atttgaaagg
     1381 ccgcgtcgat ttgcgcgacc tgcctttggt aacgattgac ggcgaaacgg ctcgagattt
     1441 tgacgatgcg gtgtttgccg aaaaaatcgg acgcaattac cgtctggtcg tggcgattgc
     1501 cgatgtcagc cattatgtcc gccccgatga cgctatcgac acggacgctc aggaacgcag
     1561 caccagtgtt tacttcccgc gccgcgtgat tcccatgttg ccggaaaacc tgtccaacgg
     1621 catctgctcg ctcaatccta atgtcgagcg tttgtgtatg gtgtgcgata tggttatcac
     1681 ttacgcgggc aatattaaag aataccgctt ctaccccgcc gtgatgcgct ctcatgcccg
     1741 cctgacctac aaccaagttt ggaaatggct ttcaggcggc atcgggcatc cgttcaaagc
     1801 ccaaatcgac acgctttaca aactcttcaa aatccttcag aaaaagcgtt tcgaacgcgg
     1861 ggcggtggag tttgacagca tcgaaaccca aatgcttttc gacgacaacg gtaaaattga
     1921 aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg attgaagaat gtatgttggc
     1981 ggcaaacgtt tgcgcagcgg attttctgtt gaaaaacaag cataccgcat tgttccgcaa
     2041 ccatttgggg cccacgcccg aaaaactcgc cgccttgcgc gagcagttcg gtctgttggg
     2101 gcttcaactt ggcggcggcg acaacccgtc gccgaaagac tatgccgcgc ttgccggaca
     2161 gttcaaaggc aggccggatg ccgaattgct gcaagtcatg atgttgcgct ccatgcaaca
     2221 ggcggtttac gaaccgcatt gcgacggaca ctttggtctt gcctacgaag catacgccca
     2281 cttcacctcg cccatccgcc gctatcccga cctgaccgta caccgcgcca tcaaagccgt
     2341 gttgaatcag caaacctaca cgccaaaaaa aagctggcag gctttgggcg tgcatacctc
     2401 gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg gaaaactggc tgaaaaccta
     2461 ctatatgcgt gacaaagtcg gcgaaatatt tgaaggcaaa atttccgggg tggcaaattt
     2521 tggaatattt gtcactttgg acgatatcca tatcgacggt ctggtgcata tcagcgattt
     2581 gggcgaagac tatttcaact tccgccccga aatcatggca atcgaaggtg aacgcagtgg
     2641 catccgtttc aatatgggcg acagggttgc cattaaagtg gcccgtgccg atttggatga
     2701 tggaaaaatc gattttgtcc tgattgccga ggggagcggc agagggcgga aagttaaatc
     2761 atccgcgtct gccaaaccgg cagggacggc ggggaaaggg aagccgaaaa ccgccgccgg
     2821 gaaaaaaaca gcccgaggcg gcaaagtaag gggaaggggc gcgtctgccg ccgcagaatc
     2881 ggggaaaaag gcaaagaaac cggttccgat taaggtaaaa aaacggaaag gcaaatcata
     2941 atgctgacgg ggcggcttgg ggagggaggg gcatactttc ccgaagccgc cgcccgattt
     3001 tttgatatgg cggccggcgc atcatttcag gccaatcgaa acgcccgtat tgaaagattg
     3061 cgtttatttc caccgccgtt ttaaaggcca gcggtattcg gcagacgggg cgcaaacggc
     3121 gttcagacgg cattttcatt ctttcggcgt gtccgtccga atggctttgc ccgtccgcgc
     3181 aatcagcccg tgcggcttgc cttgaacgga caaaaaatgc cgtctgaaac ccgaaaatca
     3241 ggtttcagac ggcattttgc cttgaaaagg ctgttcaaat cagcggtggt agttcggtgc
     3301 ttctttagtg atttgaacgt cgtgaacgtg cgattcgctc atacctgcgg aagtgatttc
     3361 cacaaattcg gctttttcgt gcatttcggc aatattggcg caacccaagt aacccatact
     3421 ggaacgcaga ccgccggtca gctggtggat gatgttcaca atcgggcctt tgtaaggaac
     3481 gcggccttcg atgccttcgg ggacgtattt gtctgcgctg tcggttttgt cttggaagta
     3541 gcggtcggca gaaccttggc tcatcgcgcc caaggaaccc ataccgcgat aggatttgta
     3601 cgagcggcct tggtagagtt cgatttcgcc cggcgcttct tccgtgcctg caaacatacc
     3661 gccgagcatg acgctgtacg cgcctgcggc gagggctttg gcgatgtcgc cggagaagcg
     3721 gatgccgccg tcggcaatca gcggaacgcc cgtgcctttg agggcttcgg caacgttgtg
     3781 aatggcggtc agttgcggca cgccgacacc tgccacgata cgggtggtgc aaatcgatcc
     3841 cggaccgata ccgactttga cggcatccgc gccggcggcg accaaatcca atgcggcttt
     3901 ggcagtggcg atgttgccgc cgatgacttg gatgtgtgga taggtttctt tcacccattt
     3961 cacgcggtca atcacgcctt ggctgtgccc gtgggcagta tcgacgacaa tcacgtccac
     4021 gccggcttcg accaaggctt tgacgcgctc ttcggtatcg ccgccggtgc cgactgccgc
     4081 accgacgcgc agacggccct cggagtcttt gttggcgttg ggaaactcgg tggtttttaa
     4141 aatatcttta acggtaatca gacctttgag ttcgtctttt tcgttcagaa ccaaaacgcg
     4201 ctcgactttg tgcgtgtgca tcagttcgcg cgcttcgtct atgcttgtgc cttcggggac
     4261 ggtaaccaga cgttcgcgcg gggtcataat ggcggaaacg ggcaaatcga cgcggttttc
     4321 aaaacgcagg tcgcggttgg ttacgatgcc gacgactttg ccgttttcaa cgacgggcag
     4381 gccggacatt ttgcgtttgc gctgcgcgcg catttccaag acttcgcgga tgagcgtcgt
     4441 cggtgcaacg gttacggggt ctttgaccac gccgctttcg tggcgtttca ctttggaaac
     4501 ggcgcgcgcc tgcatttcgg gcggcatgtt tttatggatg atgccgatgc cgccttcctg
     4561 tgccatcgaa atggcgaggc gcgcctcggt aacagtgtcc atcgcggcgg aaagcagggg
     4621 gaggttaagt gtgatttcgc gggtgagctt ggtttgaagt ttaacgtctc gcggcagcac
     4681 ggtcgaatgt gcgggaacca acaaaacatc gtcgaaagta taggcttttt ctacgatacg
     4741 cataatgctc ggtctttcag tttgtgcaag atgcacggca ttatagcacg ttaccggcgg
     4801 cttgacagtt tatattgttt gcaatatccc ccgccttcag acagcgtacg gcgcggcggc
     4861 gggataccgt ctgaaaaccg gcagggtgct tatgtcaact gttccagcaa agcctgcttc
     4921 aatgaggact ggatttttgg gtttttcagg tcggggctaa aaacggtaaa actgtcttcg
     4981 gcgcgttcat ccagtgtgga gattttggca tagcgcaggc tgacgttgtg ggcgaaaaag
     5041 acttccgcca tatcggcgag caggaagggg cggttgacgg cggtaatttc gacggagtac
     5101 cagtccggat agtcttcttc gggggtgatg gtgatgcttg gcgcgatcgg catgtagcgg
     5161 ctgcggcggc tgatgcggcg gctatggctg gagatttcgg caacggtgtg tccgtggata
     5221 aagctgttga gttcggcttc gagcgcgctt tggatgtcgg ggtagtcttc gggagcgtgc
     5281 tgcgagggga tttgcacgat gaaggtgtcg aggatgtagt cgtgttcggt aataaaggcg
     5341 cgggcggcga ggatatcgaa gccgtggcgg ctgaagatgc ggcagaggcg ggcgaacagg
     5401 cgcgggccgt tgggcatgaa aaccatgact tgaaagctgt cgcttttggg caggatgcgg
     5461 ctgcggacga tgggggtttc aaagtcgtgg actaggttgg cggcgtgcca caggatttcg
     5521 cgggactggt ggcgggcgaa gtaggcggaa ccgagcgcgt tccatagttt tttctgctgt
     5581 ttttcgggga cggcggcgcg ggtgagtaag tcggcggctt cctgccggcg gcggccgaag
     5641 agggtgtgcg ggttgccgcc gttgcctgta aggtagcgtc cggcggcatg gaagaggctt
     5701 tccagcaggc tggcgcgcca tgcgttccac agcttgggat tggtgccgcg tatgtcggaa
     5761 atggtcagaa ggtagagcgc gctgaggcgt tcgtgggttt ggacgcgttt gcagaaagca
     5821 tcgagtacgt cggggtcttg gatgtcttct ttttgggcga cggcagacat cagcaggtgg
     5881 ttttcgacca gccaggcgag caggtcgctt tcttctccgg tcaggaagtg gtcagcggca
     5941 aattggcgcg cgtctgcgat gccttgtatg gcgtggtcgc cgccgcgtcc tttggcgatg
     6001 tcgtggaaga aggcggcgag atagaggatg tcttgttttt caaaggactg catcagcgcg
     6061 gaggcatagg gcagctcgtg gctgtgcata tccaaggcaa ggcggcggac gttgcggacg
     6121 acggcgagga tgtggtcgtc cacgggataa atgtggaaca ggtcgtgctg gagcaggccg
     6181 acgatttttt cccacgcggg caggtagcgt cccaatacgc cgtagaggtt gaggaagcgc
     6241 agggtttggg tcagtccgtt gccgttgcgg aaaaagccgg cgaagcggtg gcggttttca
     6301 gaattttggt agaagctgcg gttgattttg cgcgtcgcgc cccaccatgc gcgcagggtt
     6361 tgcggttcga gcgcggtaat atcgttgcgc tgctgcatga tttcgacgat tttgaaaatg
     6421 tgttcgggcc gtctgaaaaa aatatcggtg tgccgcgcgg cgatttggtt gttgacttgg
     6481 atgtagtcgt cgtcaatccg cagggtaacg cgcaacgggg tggaggaaac gcggctttgc
     6541 agcataggcg tgaggatgcc gcccagttgt ttgacggttt taatcgcgcg gtaaaacacg
     6601 cgcatcagtt cttcgctttg gcggcggagg ttcaagcctt gataacccat gctttcggcg
     6661 acttgcggct gcaaatcgaa cagcaggcgg tcttcggcgc gattggcgtt taaatgcaga
     6721 tggatgcgga tgtgggcgag gcggcggtag ccgtgcgaaa gcataccggc ttcggcacgc
     6781 gtcaaaatcc gctgtttgag caggtcgggc aggtcggtcg ccaagccttg cgccttcgct
     6841 atccaaagca gggtgtggat atcgcgcaga ccgcccggac agcttttgat attcggctcc
     6901 aatactgccc ccgaaccttg cgatttggcg tggcggtgtt ccatctccac cagttttgcc
     6961 tcgacaaacg ccgccacatt gcgctgcgcg ttcatttttt ccgctagttt atccgccgtt
     7021 tggcggttgc caaacaaaaa cctagcctct aaaaacgctg tgtcccccgt aatatcattg
     7081 cgcacgcttt cacatagttc atcaacgctg ccgcttttta cagacggcat cagtttgcag
     7141 tcccacaggg tttggacaaa ccgggcaatc tgttcctgaa tgccgtctga aagcggggca
     7201 ggggagacaa ccgccaaatc cacatccgaa cagggataca gttcgccgcg cccgaagccg
     7261 cccaccgcca tcaggcataa cgcgctgttt tgaaaatgtt ctacccacaa tgccgccagt
     7321 aaggtttcga ctgccgccgt gtattctctg aaaaataccg acacgcgctt ggctttcaaa
     7381 taatgcgctt cggcggcatc gcgctgctgt ttgaaggttt ccagtgctga agacaggttt
     7441 gcaggcattt ttattctttc gattggcggg aaaaaaggag acggatggtt cggcggtcaa
     7501 ataccgcttt cagacggcat ttgtcgggta tcggcggaat tggtcatcag gttcaaccgt
     7561 tcctgcggcg gcagcagcaa ctgatacgcc gccacgccca aagccgccgc cattttttcg
     7621 atattcgaca gggcgatgtt ccagcgtttg cgttcgactg ccgacacata agtcctgtcc
     7681 aaaccgcatt gccgcgccaa ttcttcttgc gaccaaccct tgttcacgcg gaaaagccgc
     7741 atattgtatg ccaataccgc ccgcaaatcc tgttcggcag gcagttcggc aggcagagtc
     7801 aatttgttgc ccatcatttg cttccggatg aatgttaaag ttaagcattt gctgttacgg
     7861 attttacttc acataaaagc caaaaaaaag gggtggcggc agccacaccc aaacacacac
     7921 acatcaagag gaaagaggta aaatcaagac agattgggaa acagcaaaca agcaatctag
     7981 atttcatatc ggtattatta aacaaaaacc gttatcgagt attgattcaa atcaatttag
     8041 atttgattcg caaaccggca aagaaaaacg gcatacccgt ttatacggat atgccgtctg
     8101 atgatgcagg ggactgctat atttggcagt ggtttgtgtt ttagtcctct tgcgccgctg
     8161 tttgcaggta gttcggcaaa ccaattttgc cgatcagttc ctgctgggtt tcaagccagt
     8221 cgatgtgttc ttcgttggtg tctttttgtt tttccaacaa atcacggctg acgtaatcct
     8281 gttgcgcttc tgctgtagcg atggcagcaa gcagggcttc gtgtttttcc tgttctttgg
     8341 tcaaatcgca ggcaatgatt tcttcggtag attcaccaat caacagtttg cccagttctt
     8401 gcaggttcgg caggccttcg aggaacagaa tgcgctcgat caaatcgtct gcagctttca
     8461 tttcaacgat ggactgtttg aagaaatgtt cgcccagttc tttaaagccc cagtttttca
     8521 aaatacgggc gtgaaggaaa tattggttga tggttatcag cagcaagcct aagtttttgt
     8581 tcagctcgcg gataactaaa cggtcgcctt tcatacggac tccttttatt ccgaatacag
     8641 cttacagttg gctttggtag tagttgcctt cgccaatcag ctcgatcagg cgcagctgct
     8701 gttccaacca gtgtgcgtgg tcttcttcgg tatctttcag ttgggcaacc atcaggtcgc
     8761 gcgtaacata gtcttgagcc tcttcgcaca gtttgatgcc ttttttcaac gcatcacgta
     8821 cttcatattc ggtttgcagg tcggctttga ggcaggaaac cacgtccgtg ccgatattca
     8881 gttcggcgcg taccattttc ggcgtaccgc ccagcatcag gatgcggcgg ataaagtctt
     8941 cggcgtgtgt ggtttcttct tccatctcgt gattgagacg ttcaaaaagt ttggtgtagc
     9001 cccattcgga gtagaggcgg gagtggataa agtattggtc gcgtgccgcc agctcgccag
     9061 acagcaattc gttcatataa tcaacaacag cttgattgcc ttgcataata tctctctttc
     9121 cgtaacttgg ttttcggtat gtctgaacag catctgtgtc gctattcggg atgcgtgcat
     9181 tgtatgcaaa agctgtcggc atgacaaatt ttctatttaa aatacaaaca attatcaaaa
     9241 gaaatagggc gtattccccg atgccttcca aatcagtatg ctgtttctta tcggtttttt
     9301 gtgctgttaa aactaagaat ccatatcatc cataaagaac atttacactt gttaaccata
     9361 gcgaaaaaca aatagagcac ggttttttat caaaatttat aatgaatcgt tctcattaac
     9421 cgacagattc ttttgagatt atcggatttt gggaataaat tatgccgtct gaggggcttt
     9481 cagacggcat agacaattaa agctgattaa acaacgcggc agacagcagc gtaggaaatt
     9541 attttttact tcggaacggt attttgggtt taatgatgcc cgcaaccgcc gtggctttcg
     9601 cgtaaggctt cggcagcctg ttcgtcggcg tggtagctgg aacgtaccat cgcgccgatg
     9661 gcggcattgc tgaagcccag ttcgtatgct tctttttcaa agattttgaa ctgctcgggc
     9721 gtaacgtagc gcaagacagg caagtggccg tctgaaggct ggagatactg tccgatggta
     9781 atcatctcga tattgtgcgc ccgcatatcg cgcataattt cgcgcacgtc ttcgtctgtt
     9841 tcgcccaagc cgaccatgat gccggatttg gtcgggatgt gcggcatcat ttctttataa
     9901 cgttttaata agtctaaaga atgttgataa ttggcaccgg gacgggcttt tttgtacagg
     9961 ctgggatggg tttccaagtt gtggttcatc acgtcgggcg gagtttcggc aaggattttc
    10021 agtgcgatgt ccaagcggcc tcggaagtcg gggacgagga tttcgatttt ggtgttcgga
    10081 ctagtctcgc ggatggcttt gatgcagtcg gcgaaatgct gtgcgccgcc gtcgcgcagg
    10141 tcgtcgcggt cgacggaggt aatgacgaca taacgcaggt tcatggcttt gacggattcg
    10201 gcgaggtttt tcggttcgtc ggggtcgagc atattcgggc ggccgtgtcc cacgtcgcag
    10261 aacgggcagc ggcgggtgca gatgtcgccc ataatcatga aggtcgccgt gcctttgctg
    10321 aagcattcgc cgatgttggg gcaggaagct tcctcgcaaa cggtgtgcat tttttgttcg
    10381 cgcaaaatgt ctttgatttc aaagaatttg cgcgatggaa gtttggcgcg tatccattcg
    10441 ggctttttta atttttcctg aagggggacg actttgatgg ggatgcgggc ggttttgtcc
    10501 gcgcctctga gtttgatgcc gcgtttgggg tcgtcggttt tgatttcact cattgttgtc
    10561 tgctttcggt gtgaattgtg tttcaaggtg tgcggtgagt ttggcggcga cttcgtccgg
    10621 cgtggggcag ggttggacaa aatccgcgat ttgcgtcatt tccataccgg cgtagccgca
    10681 ggggttgatg tgggtaaacg ggcttaaatc catattgacg ttgagcgcaa gcccgtgata
    10741 gacggagccg tttttgatac gcagccccag tgaggcgatt ttgcgttctc cgacataaac
    10801 gccggggcgt ttggggtctg ccgccgcttc gatgccgtat tctgccaatg tggcgatgat
    10861 gctgttttca agcgcggaaa cgatgtttct aacactggtt ttgcgccgtt tgaaatcaat
    10921 catcgtataa acgaccaatt gcccgggccc gtgataggta atctgcccgc cccggtcgat
    10981 ttggacgacg ggaatgtcgt cgcgaatcag caggtgttcg ggttttcccg ccagtccttg
    11041 tgtgaacacg ggcgggtgtt cgacgaccca cagttcgtct tcggtgtcgg cattccgtcc
    11101 ggcattaaag gttttcatcg cttcaaaagt cggcagatat tcgaccaaac ctttgtgtat
    11161 gattttcatc tcaaagtacc actttgacca gttcgtgcga agtcagcgca cggtagatgt
    11221 tgtccaattg ttcttggttt tcaaccttta cctgtacggt ggcgccagta tagttgcctt
    11281 tgctgctcgg acgcgtggtg atgtggtgcg cctgcgtgtc gggggcgtgg aggcggacgg
    11341 tgtccaatac cgcctgctcg aactcgggat gcaccgcgcc cattactttc aatgggaagg
    11401 tgcagggaaa ttcgatgagg gatgttttgt ttttttgttc ggtcatgatg tgctgccttg
    11461 ttgtgtacgg tatgccgtct gaaggcgggt ttgcctttca gacggcatcg gatgtgcgtt
    11521 attttagcct aaaccgcgat aacaggctat cgggaaggcg gaggcttttt tgacggcgcg
    11581 gcggttctgc tatactggcg cgcaatatta tttttagaag ggtggttttt atgtatcgga
    11641 ggaaagggcg gggcatcaag ccgtggatgg gtgccggtgc ggcgtttgcc gccttggttt
    11701 ggctggtttt cgcgctcggc gatactttga ctccgtttgc ggttgcggcg gtgctggcgt
    11761 atgtattgga ccctttggtc gaatggttgc agaaaaaggg tttgaaccgt gcatccgctt
    11821 cgatgtctgt gatggtgttt tccttgattt tgttgttggc attattgttg attatcgtcc
    11881 ctatgctggt cgggcagttc aacaatttgg catcgcgcct gccccaatta atcggtttta
    11941 tgcagaacac gctgctgccg tggttgaaaa atacaatcgg cggatatatg gaaatcgatc
    12001 aggcatctat tattgcgtgg cttcaggcgc atacgggcga gttgagcaac gcgcttaagg
    12061 cgtggtttcc cgttttgatg aggcagggcg gcaatattgt cagcagtatc ggcaacctac
    12121 tgctgctgcc cttgctgctt tattatttcc tgctggattg gcagcggtgg tcgtgcggca
    12181 tcgccaaact ggttccgagg cgttttgccg gtgcttatac gcgcattacg ggtaatttga
    12241 acgaggtatt gggcgaattt ttgcgcgggc agcttctggt gatgctgatt atgggtttgg
    12301 tttacggctt ggggttggtg ctggtcgggc tggattcggg gtttgcaatc ggtatggttg
    12361 ccggtatttt ggtttttgtt ccctatttgg gcgcgtttac aggactgctg ctggcaaccg
    12421 tcgccgcctt gctccagttc ggttcgtgga acggcatcct atcggtttgg gcggtttttg
    12481 ccgtaggaca gtttctcgaa agttttttca ttacgccgaa aatcgtggga gaccgtatcg
    12541 ggctgtcgcc gttttgggtt atcttttccc tgatggcgtt cggacagctg atgggtttcg
    12601 tcggaatgtt ggcaggattg cctttggccg ccgtaacctt ggtcttgctt cgcgagggcg
    12661 tgcagaaata ttttgccggc agtttttacc ggggcaggta ggcggttccg aaacatatag
    12721 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat aggacaacgc
    12781 cgtactggtt tttgttaatc cactatattt gaagcggaat acaaccctgc ccgagtttaa
    12841 ataaaaatgc cgtctgaacc ccgaaaacag agcttcagac ggcatttcat cacggcttat
    12901 ttggcggttt tgctgctgtc gataattttc ataccggcag aaatcaggct gccgatgtcg
    12961 gcaacattgg cgggcataat cagcgtattg ctttctttgg caagattgtt gaacgcggcg
    13021 acgtattgtt ccgcaatctt cagattgacc gcatccgcac cgccttgggt ttgaagggcg
    13081 gcggcaattt gacggatggc ttcggcattg gcttcggcaa caaggcgcaa ggattccgct
    13141 tcacctttgg cgcggttgat gcgggcgatt ttttcggcat ttgacgcatt gaccgcagcc
    13201 tgagcctcgc cttcggattg ttggatttcg gcttcgcgct gaccactggc aaggttgatt
    13261 tgttcgattt tacgaccttc ggattcggcg atacgggcgc gtttttcgcg ttcggcagta
    13321 atttgcgcct gcattgagcg aaggatttct tgcggcggca ccaagtcttt aatctcataa
    13381 cgcaaaacct tcacacccca agccccggcc gcctcgtcca aagccgcaac aacagtactg
    13441 ttgatttcgt cgcgttcttc aaacgttttg tccaactcca tacgtccgat aacggaacgc
    13501 agcgtcgttt gggcaagctg ggtaatcgcc ataatgtagt tgctcgaacc gtatgaggcg
    13561 agtttggggt cggttacttg gaaatagatg ataccgtcaa cagtcagctg cgtattgtcg
    13621 cgcgtgatgc agacttggct gggtacgtct aaagggattt ctttcagcga atggcggtag
    13681 gcgacgcggt cgataaaggg aatcaaaata ttcaaaccgg ccgtcagggc gcgatggaaa
    13741 cgcccgagcc tttcgacaac gtggacttcc tgttgtggga tgacaacaaa ggatttgaaa
    13801 ccgaaaacgg cgacggctac caacaagata atgaaaaatt ccataattcc tccgagtgtt
    13861 aagggtgtgt gataataaga aggttgcctt ccttgcggac aatgagggcg cgcgttcccg
    13921 gttcaagctc ttcttgcccc gtattttgcg cctgccagtg cgtaccgcga taaaaaactt
    13981 cgtaacggtt gccgcctacg tgccggagga tttcggcata ttgcccggca tccaaatcct
    14041 gatatgaatc cgtttcaact tttcccacgg cggttttggc gtgtacgaac caaataccca
    14101 gcgcggaaag cagagcggcg gtcaagacgg cggcaggcgt gctgccggtc agcccgtaag
    14161 caatgcccga acccgccaaa gccgcgctga caaccaaaag ataaaccgtt cccgtcaata
    14221 attcgatgat taagacggca acagcggcaa caaaccatac agtcatacat ttccccacaa
    14281 agcgcgtcgt ttgacaaaat aacgcaatat cagcagtata gccgaatttg aaaggatagg
    14341 gcagatatgg acacttggca cgatgcactc ggcggcgaaa aacagcagcc gtattttcag
    14401 gaaattttaa atgcagtcag gcaggaacgt ttgtcgggac aaatcatcta tccgccggcg
    14461 gcggatgtgt tcaacgcatt ccgcctgaca gcgttcgacc gggtcaaagc cgtcattctc
    14521 ggacaagatc cgtatcacgg ggcagggcag gcgcacggtt tggcattttc cgtccggcag
    14581 ggtatccgca taccgccgtc tttactcaat atctacaagg agttggaaac cgacatcgaa
    14641 ggcttttcca ttcccgcgca cggctgtctg acagcgtggg cggagcaggg cgtattgctt
    14701 ctgaacacgg ttttgacggt gcgtgcagga caggcgcatt cgcacgccct tttaggctgg
    14761 gaacgcttta ccgataccgt catcaggcag cttgcggcgc accgcaagca ccttgtcttc
    14821 atgttgtggg gtgggtatgc gcaacaaaaa aggaaactga tagacagtca aaatcatttg
    14881 atactgaccg caccgcatcc gtctcctctg tcggcatatc gcggtttttt cggctgccgc
    14941 catttttcac aggcaaacag ctatttgagc cggcacggta tcgatccgat aaactggaag
    15001 ctgtgaatgc cgatatagcc gttgccgccg gcgtgttaaa atcgcgtttg atttgtaatt
    15061 tccatttatt aggcaaaacc ttatggcaat caaaaaaaat cagctttatg catcactttg
    15121 ggcgggctgc gacgagttgc gcggcggtat ggatgcgagc ctttataaag aactatgtgc
    15181 ttacgctatt gtttttaaaa tatgtttctg ataagcataa gtacggcggc ggcatgattg
    15241 agctgcacgc cggtacgact tttgacgaca tcgtcaaact caaaaacacc gccgacatcg
    15301 gcgaccgcct gaataagatc atcgcccaaa ttgccgaagc caacgactta aaaggcgtga
    15361 tcgacgttac cgacttcaac gacgaagaca aactgggtaa aggtaaggag atgatcgacc
    15421 gtttgagcag gcttgtcggc atttttaaaa agctcaacct ttcttccaac caagccgaag
    15481 acgacgattt gttaggtgat gcctacgaat acctgatgcg ccattttgcg accgagtcag
    15541 gcaaatccaa agggcagttt tacacgcctg ccgaagtctc ccgcattatg gcgaagatta
    15601 tcggaatcag cgcagattgc cgtccagcac cagcgtttat gacccgacct gcggctcggg
    15661 ttcgctgttg ctcaaagccg ccgcccaagc cggcagccaa atcagccttt acggtcagga
    15721 aaaagatgtg gcaaccgcgt cccttgcccg tatgaaaatg attttgcaca acaacgaaac
    15781 cgccgaaatc aacaccggga acaccttgtc cgattcgtct ttccgtgatg aaaacgacgg
    15841 gcttaagacc ttcgattttg ccgttgccaa tccgccttgt cccgcccgaa aaaaacggcg
    15901 attacgcctt tttgctgcat ctgctcaaaa gcctgaaacc aagcggcaaa ggtgcgatta
    15961 ttcttccgca cggtgtgctg tttcgcggca atgccgaagc gcgtattcgc acggaattgc
    16021 ttaaccttga ccttattaaa ggcattatcg ggctgcctgc caacctgttt tacggcacgg
    16081 gcattcctgc ctgcatcatc gtcatcgaca aagaacacgc ccaaaccgcc caatttgccg
    16141 aagagggaac aaaccaagtt atcagcggcg gcagcgtgtt tatgattgac gcatcgcgcg
    16201 gcttcattaa agacggcaac aaaaaccgtc tgcgtgagca agacattcac aaaatcatcg
    16261 acactttcac aaacctcgtt acagccgtat ggtgcattta agcgaaatcg cagcacaaga
    16321 ttacaacctt aatctgcctc gctatatcga cagcggagaa gtcgaagacc tgcaaaatct
    16381 cgccgcctat ctatctttat ggcggcatac ctgcgcacga tatggacgca ttggaagcct
    16441 attggcaagt tttaggccgt atgaaaaacg agttgtttgc cgaacacgat ggccgcttta
    16501 ccactataca acggaatcga ttgcaaatct ttcccactct caacagctta aaatcctgcg
    16561 ggattggtgt ggaatttagg gctaatctag tacagcccca aatttaatcc actataaaat
    16621 cgaaagcagc caaatcaaag cccatatatt ggcgcacccc gattacgccg ccttcaaagc
    16681 cggacacctg gcaaagtttg ccgcgtggca cactcaaaac gaccttgccg ccatccaacc
    16741 gggcaggctt atccggaaat ggagcgaaag cctgctggac gcgttcaaac ccggcagcct
    16801 gattgaagaa tacgatttct accaaatcct gacggactac tgggcggaaa ccctgcaaga
    16861 cgatgtttat ctcatcgccc aagatggctg gaaggcggtt aaaaacctgg ccgaaatcac
    16921 caaagaaagc gatgaagccg cgaacctgac cgtcgtcttt gaggaaaccg aaaccgacaa
    16981 aaaaggcaaa gccaaaacca agcgcatcag caaaaaatac cgcagcgaag tcatcgcccc
    17041 cgagctggtt gcccgccgct acttttcaga cggcatcgcc aagctggaag aaaaacaaag
    17101 cgagctggaa cgcctaagcc aagaattgga aaaccacata gaagaacacg gcggcgaaga
    17161 gggtgcgctg aacgacgtat tggatgcaaa aggcaaactt tccgccaaac ttctgaaaac
    17221 cgcattggaa gaaagcggca tagaagaagg cgaacgggct gttttacaaa ccacccaaac
    17281 actgatgacg caggaaaaag ccgcgaaaga cgcagtcaaa acccaaatcg aagccctgaa
    17341 ccttgccgta ttcaaacaat tcggccgact ttccgaagcc gaaatcaagc agcttgccgt
    17401 tcaagacaaa tggcttgccg atttacaaag ccgaatcgaa aatcgcttgg aaaacagtat
    17461 tcagcagctt atcagccgct tgaacacgct ggaagaccgc taccgcagcc cgatggccga
    17521 gcttgcccga gaagtggaaa agtggcaaag caaagtcaat gcccaccttg aaaatatggg
    17581 ttttggaggc tgaaatggcg gcacagacag gctataaggc gagcgggttt tgagaccttt
    17641 gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatc gcggttcggg
    17701 tgtccgcacc gcttaatacg tcgtcgtcca cgaactgacc catttgctcg aacgccatcg
    17761 caacgcccgt tttatggcgc atatggacaa ctttctccca aactggcaaa gcatcaaaca
    17821 acagcttaat gccttggagt tatttgcaca aatatataat ttaacataat atacattatg
    17881 cgaactatcg gaaacagttg tacgtgtccc tgtggtcttt ccaagtagga aaattaaagt
    17941 atggacaatg cggctgaatg tatagcccgg agcatccgcg tatccgaagg tactgaacga
    18001 tatgccctgc atccctttcg atgcttccat ccagccggta ttgggaaata cgttgacctt
    18061 caatataata tacaggcagc cgacacacag gatatgccgc tgctttttca tcatttcttc
    18121 tgtcaaatcc ttggaacggt cgatttgaaa acacggcttt acatacgtcc caattctctt
    18181 tgcagtgctt ggatattttg ctgcctgtcc aatacattgc tttgtaatgc atttatttct
    18241 tgatggttga tgttgccgcc ctttgccaga cgtgcttgtg ataacatttt ttgggcttca
    18301 gtcagggctt tgcgttcgtt gctcaattct gcttcgagaa tggagcgtct gctgttgttt
    18361 acaggtgctt gttgaggcgg gggcgtattg gattttgccg gcttggatac tgttttgacc
    18421 ggggctttat atttgacagc ctgtccgccg tttgacggtg atggtgtcgg ttcggatgtt
    18481 tggggcggga tatagcgttc gctgctgtag ttgccgattg ggggcaaatc ggttgagagg
    18541 cagcttttgg acggcttggt ggtgtaaacg gtttctccgt tgattgtgca ggtgtagatt
    18601 ttggccgcat tcgcacccaa tgggcttgaa atcagggaaa agttgattag gatgaagggg
    18661 agttttgatt tcataatgtg cttgatgctc ggataattat ttgatttttt ggtatttttt
    18721 gtaatgcttt cttggcgttt tacgggcttt tcttttcggt tggttgcttt tgattttctt
    18781 tcggggcttt ggcagcctgc ctgttctttt tcccggcagg ttttttgttt ttctttttac
    18841 tttctatgtg tattttggct tcttaactga gttttttaat tttcaggcgg tatccgcctc
    18901 cctgatggct gctgatttta ggtaaatccg catcggcgca cgatcctgct ggggctgatg
    18961 tatatacgct tttgccattt gagttgcaat ggtatacgga ggcttgcgcc gccgcgccag
    19021 aaagggacag gagacccaag gcggcaaaaa gtctgatgtt tataccggta aaaggcggtg
    19081 ataacgcccg aattataccg ttattggcag gcaaagataa gcaccctgcc cgcgcttctt
    19141 tatcgctcgg caaactgttt ctcgatttca gtttcaatcc gttccaattc ttcttgacaa
    19201 gccagccaaa cctcttcgat ttgggcaagt tgtgttttga cttttgccag ctgggataag
    19261 gtattttgca atttttcttt gtttgcttca aggtaagcgt cttcttgtgc taaaaatgct
    19321 tcacatgccg tctgaatttc ggaaagctgc gccatttctt tttcggcacg gtctattttc
    19381 tgctgtatcg gcttgccgcg tcgggctttt tcctgacgga tttgcgcttc gatgcgcttg
    19441 gtgtctttgc ggctttggct ttgtgcggat gctgctggtg cggcggcgtt ttcctgtgcc
    19501 aaacgccatt ggcggtaatc gttcaaatcg ccgtcgaagt tcttcagacg gcctttgtcg
    19561 atcaggagga agctgtcggt agtcgcttca agcaggctgc ggtcgtgcga tacgatgatt
    19621 aaggcgcctt ggaaactttg cagagcgagc gtcagggcgt ggcgcatatc caagtccaga
    19681 tggttggttg gttcgtcaag cagcagcagg ttcggctttt gccagataat catggcgagg
    19741 gcgaggcggg ctttttctcc gccggaaaaa ggttcggttt tctgcaacgc catatcgccg
    19801 gcaaagttga agccgccgag gaaatttcgg atttcctgtt cgcgtacttt gggagaaagc
    19861 tgctggatat gccaaacagg gttttggtcg gcgcggatgg tatcgagttg gtgttgggca
    19921 aaatagccga tattgagttt ttcggaacgg acgatgctgc cggaaagtaa atcgattttg
    19981 cccgccaagg ctttgataaa ggtagattta ccgctgccgt tgacacccaa tagcccgtag
    20041 cgtgcgccgc tttccagcga cagggtaatg tcatgcagaa cggttttgcc ttcgtaaccc
    20101 aaatcagcgt gctccagctt cagcaaagga ttgggcagat gatcgggatg gtaaaactca
    20161 aaggaaaact cgctgtccag atgcgcgggc gcaacgcgtt ctagctttgc caaagccttc
    20221 atgcggcttt gcgcttgaac ggctttggtg gctttggctt tgaagcggtc gataaaggat
    20281 tgcaaatgtt tgatttgcgc ctgctgtttg acataggcag cttgttgttg cgcgagacgc
    20341 tgcgcgcgtt cgttttggta aaaatcgtaa ttgccgccgt attgcgtgaa tttttgctgc
    20401 gataattcaa tggtttgggt agttgccgcg ttgagaaaat cgcggtcgtg ggaaatgatg
    20461 atttgcgtgc agggtaaaga agcaagatgg ttttccagcc acaagacggt ttccaaatcc
    20521 aagtggttgg tcggttcgtc caaaagcagc aaatcggcgc ggcaaatcag ggcttgggcg
    20581 agattgaggc gcatacgcca gccgccggaa aaggatttga cggggcggct gtgttcttct
    20641 tgtgaaaaac ccagcccgtt caacaatttt gccgcgcggg ccggcgcggt ataagcgtcg
    20701 atttcttcca atttggcatg atattccgcc tgcttcatgc cgtcattttg cgcttccgcc
    20761 tgcctcaatg ccgtctgaaa agcctgcaac tcggcatcgc cctgcaaaac gtaatccaaa
    20821 gcggaaatat ccaaatcggg cgtttcttgg gaaacggaag cgagccgcca gtttttcgga
    20881 atcgagacat cgccgccgtc ctgagtgatt ttacccttga ttaaggcaaa caggctcgat
    20941 ttgcccgttc cgtttttgcc gatcaaaccg acgcgctgac cgggattgac ggtagcgttg
    21001 gctttgtcga gcaggacttt caaaccgcgt tgcagggtga ggtttttgat ttcaatcata
    21061 acggaaacat cgtcgggcgg aaaaagcccg tattttacct gaaagtcagt gccgatgccg
    21121 tctgaaatgg gaaatttacg gctgaagcca agcccaagcc ctgcgccctt ccgagtgcag
    21181 gaaaaccaat gtcctgaatg ccgaatcggt attcatacat tccacgccga ttccgattcg
    21241 agaaaaatcc gccatgattt tgggatggat aaactcctga gccgcgcccg tcccgataat
    21301 caatatttcc ggatagtcaa caggtttgat gtcggacaac aggttttccg gagtcagatc
    21361 ggacaaggtt cggcattgcg acaggcagac cgaatcctta tgtacaagca cgggtttatg
    21421 gaaactttga cccgccaatc ggatttcacc cgaccggtgg ccatactctg taaaatgtcc
    21481 gtctatcgga ttttcttcaa acaacatttt tttaccccgt cgccgcatca tctacaccga
    21541 aaggggtgca aaatcaggcg aattcatgta ggattggcag atttcatctg acccgcctgc
    21601 cgatttcaga ctgcatttga ttcaaagtgc ggcacaatta tatcggcagc ggatattttc
    21661 gtctttcaat atttacattt cagtcggctt acaaggagac acaatgaagc cagtaaacat
    21721 cggtctttta ggtttaggta cggtcggcgg cggtacggct gccgtgttac gggacaatgc
    21781 ggcggaaatc agccgccgtt tgggacgcga agtgcgcatc agtgccgtat gcgacttgag
    21841 cgaagaaaaa gcccgccaaa tctgcccgtc cgcagccttt gtcaaagacc cgttcgaact
    21901 ggtcgcgcgt gaagacgtcg atgtcgtcgt cgaattgttc ggcggtaccg gcattgccaa
    21961 agatgcggtg ttgaaagcca ttgaaaacgg caaacacatc gttaccgcca acaaaaaact
    22021 gctcgccgaa tacggcaacg aaatcttctc gctggcggaa gaaaaaaatg tcatggtgca
    22081 atttgaagcc gcagtagcgg gcggtatccc aatcatcaaa gccctgcgcg aaggtttggc
    22141 agcaaacagg attaaatcca tcgccggcat tattaacggc accagcaact tcatcctctc
    22201 cgaaatgcgc gaaaaaggca gcgcgtttgc cgatgtactg aaagaagcgc aggcattggg
    22261 ttatgccgaa gccgatccga ccttcgacat cgaaggcaac gatgcgggcc ataaaatcac
    22321 catcatgagc gcactggcat tcggcacgcc gatgaacttt tccgcctgct acctcgaagg
    22381 catcagcaaa ctcgacaacc gcgacatcaa atacgccgaa gaacttggct atcgcatcaa
    22441 actgttgggc attacccgca aaaccggcaa aggcatcgag ctgcgcgtcc accctaccct
    22501 gattcccgaa agccgcctct tggcaaacgt caacggcgtg atgaacgccg tgcgcgtcaa
    22561 cgccgatatg gttggcgaaa ccttatatta cggcgcgggc gcgggcgcat tgccgaccgc
    22621 ttccgccgtg gttgccgata tcatcgacat cgcccgcctg gttgaagccg ataccgccca
    22681 ccgcgtaccg catctggcgt tccaacccgc gcaagtccaa gcgcaaacca tcctgcctat
    22741 ggacgaaatt accagcagct actacctgcg cgtccaagcc aaagacgaac cgggcacgct
    22801 ggggcaaatc gccgcgctgt tggcacaaga aaacgtgtcc atcgaagcac tgattcaaaa
    22861 aggcgtgatt gatcagacca ctgccgaaat cgtgattctg acccacagca cggtcgaaaa
    22921 acacatcaag tcggcaatcg cagccatcga agcactggat tgcgtggaaa aaccgattac
    22981 catgatccgc atggaaagcc tgcatgactg agccgaaaca cgaaatgccg acggaagagc
    23041 aggttgccgc gcgcaaaaaa gcaaaagcca aaatccgcac catccgcatt tgggcgtggg
    23101 tcattttggc gttgctcgct ttaaccgccc tgctctccca atgcgcgatg tccaaaccgc
    23161 aggcaaaaca gaaaattgtc gagtcttgcg tgaagaatat tccgtttgcc gaaaaatggc
    23221 aaaacgattt gcgggcccgc ggtttagatt caaacaatac ccgcctcgcc gtcgactact
    23281 gcaaatgtat gtgggagcag cctttggaca gattgagcga gaaacagatt agatccttcg
    23341 gcaaactcgg cgcacaagaa cagcttgacc tgctcggcgg cgcaaatgcc tttgaagcac
    23401 gtgacaagca gtgtgttgcc gatttgaaat cagaataatg tggaccgata aaaaagccga
    23461 ttctttaaag aatcggcttt tttcataaaa aacggcttac agtgcgtctt tcaatgcttt
    23521 acctgcacgg aatttaggcg ttttggcggc ggcaatggtc agaggctcgc cggttttggg
    23581 gttgcggcct tggcgttccg cacgttcgcc cacgtagaaa gtaccgaaac cgaccaaagt
    23641 aacggtgtcg ccttgtttca gggcggtggt tactgcattg gtagtggcat ccaaagcttt
    23701 ttgtgcggcg gctttggaaa tgtcggcttc ttgagcaatc gcttcgatca attcagactt
    23761 gttcacaatc agtcccttcc tgtcttaaaa aatgatgaaa tgcccgaata ctcggggttt
    23821 gtactgcttg agcaactttc gctttatagc aattctgaaa ttgccgtgtc aagcaaaaaa
    23881 tacggaatca ccctatttga caggctttca ggacgaaacc gcatttttac aacacatttc
    23941 ctgcgtttca atgtttggtt gccctgctgc ggggttttgg ttttgaagcg gattccgccg
    24001 ccgcttccgc accagaaggt tctgcccaag gctcaggctg gctttccaaa cccagagcca
    24061 atacctcgtc tatccatttg accggatgga tggtcaggcc ggttttcacg ttttcaggga
    24121 tttcttccaa gtctttgagg ttgtcttttg gaattaaaac gtgtttgatg ccgccgcgaa
    24181 gcgcagccaa cagtttttcc ttcagaccgc caatcggcag gacttcgccg cgcagggtaa
    24241 tttcgcccgt catggcaaca tcggcgcgca ccgggatttt ggtgaatgca gacaccatcg
    24301 ccaaagtcat ggcaatgccc gcactcggac cgtctttcgg cgtcgcacct tcgggaacat
    24361 ggacatggat gtcttttttc tcgtaaaaat caggagccaa gcccactgat tccgcgcggg
    24421 agcggacaac cgaccacgct gcggacacgg attccttcat cacatcgccc aactggccgg
    24481 tgcactgaat cacgccctta cccggcaatg ctgcggcttc gacggtcagc aattcgccgc
    24541 cgacttccgt ccacgccaaa ccggttactt gtccgatacg gttttcgctt tcggcaacgc
    24601 cgtaatcgaa gcggcgcaca cccaaatagt cgtgcagatt tttctcattt actttaaccg
    24661 ccctgggctt ggcttttccg gtttttttgg tttttgagtc ttcgtccaag gtaatctgca
    24721 tcaccacctt gcggcagatt ttggcaatct cgcggtcgag cgaacgcacg cccgcctcgc
    24781 gggtgtagta acggataata tcgcgcaccg cgctttcttc gattgccaat tccccttctt
    24841 ttacgccgtt gcgcttcatt tgcttcggca cgaggtactg catcgcgata ttgatttttt
    24901 cgtcttcggt atagccggac agacggatga tttccatacg gtcgagcagc ggtgtcggga
    24961 tattcaggct gtttgaagtc gcaataaaca tcacatcgct caaatcgtaa tccacttcgg
    25021 cataatgatc ggcgaacttg ttgttttgct cgggatcaag cacttcgagc aacgcgctgg
    25081 cgggatcgcc ccggaagtcg ctacccaatt tgtcgatttc gtcgagcagg aacaatgggt
    25141 tttttacgcc ggcttttgcc atattctgca aaatcttacc gggcatggag ccgatatagg
    25201 tgcggcggtg tccccggatt tcgctttcgt cgcgcacgcc gcccaaagcc atgcggacat
    25261 atttccgccc cgttgctttg gcgatggatt cgcccaaaga ggttttgccc acgcctggag
    25321 ggccgaccag gcacagaatc gggcctttga gtttgtccat acgtttttgg acggcgaggt
    25381 attccaaaat ccgttctttg actttttcca ggccgtagtg gtcggtatcc agcaccaatc
    25441 cggctttggc gatgtctttg ctgacgcggg attttttctt ccacggcagt tcgagcaggg
    25501 tgtcgatgta gttgcgtacg acggtggatt cggcagacat cggcggcatc attttgagtt
    25561 ttttcagttc ggacaggcat ttttcttccg cttctttgct catccccgcc tttttgatgc
    25621 ctgcttccaa ggcatccagt tcgccgtttt cgtcttcttc gcccaattct ttgtgtatcg
    25681 ctttaatctg ttcgttcaga taatattcgc gctgggattt ttccatttgg cgtttgacgc
    25741 gtccgcgtat gcgtttttcg gcctgcataa tgtcgagttc ggattccagc tgtgccagca
    25801 ggaattccat ccgtttgccg atttcgggaa tttccaaaat ctgttggcgt tgcgccagtt
    25861 tcaactgcaa atgcgctgcg accgtatcgg ttagccggct gttttcggca atgccgttaa
    25921 tgctgccgat aatttcggcg gggatttttt tattgagttt ggcgtattgt tcaaactgcg
    25981 ccaacagggt gcggcgcacg gcttcgaggt cggtattgcc gcccgtgtct tcttccacga
    26041 ccgtctctat atgggaaacg aacagaccgc ccgtgtcttc aatggtcaga acacgtccgc
    26101 gatacagccc ttcgaccaat acttttaccg tgccgtcggg tagtttcaac acttgcagga
    26161 cttgtgcgac cgtaccggtc tgatacaggt cggcggcaac cggttcttct accgccgcat
    26221 cggtttgcgc caacaggaaa accggctcct cgcgggtaat ggcgttttcc agtgcggcga
    26281 tggatttcgg cctgccgaca aacagcggca gaaccatatg cgggtaaacg acgacatccc
    26341 gcaaaggaag ggttgccaag gcggcatatt cctcaaaatg cttttctttt tgtgtcatag
    26401 gtactctctt gtgtctgaca gattgccgat tttcgcgtac attggggttg aaggtattat
    26461 ttcaagcata tgtggtttat ttatggagtt tgatgcgatg ccgtctgaaa cattccggct
    26521 tcagacggca tgggcttgga aagacaaggc gggaacaaaa aaccgttctg tgttgccgct
    26581 ccttgctgta ccatccgtat ggtttgcggt tctgccgccc tattttcaaa acatgacgca
    26641 ggtattccat gtcttctttt atttttcccg ataacgggtt cgaatggcgc aacgaaagtc
    26701 tgcaaaagcc gcccaaaggt tggcattatg tccgcgaaag cggtgcaaac ggcattttga
    26761 aggctgccta tcaaaatatt gcaactgtct ggcagggcga tttccacaat gccaaacagg
    26821 tgctttccgc aatgaagaag aaggtccgca aacctgccgc cgtccgttcc gatgcggata
    26881 tcgccgccct tttccacgcc caccgtatga agcaggcgca gcagagccgt attctgaata
    26941 tgcttgccgt tgaaatccgc cccggttttg tgttggacaa caaacgcgcg cccgatatac
    27001 gcgccgccct gctcgacgtg tacggagagg cggacggcaa accgtttttc ctgccgctca
    27061 atctgctgct ggggtttatg ggtgcgcacg agtggcataa gaaaggggtt gccgttccgc
    27121 agctgggcgg cagcatacac gttcctttcg gcgtattctc gccgttgcgc ggcgaatacc
    27181 tcgatctgct cgcccatgcg ccgtcaacgg gttttcagac ggcattcgat atcgggacgg
    27241 gctcgggcgt gcttgccgcc attttggcga aacagggcat tccttccgtc atcggcacgg
    27301 ataccaatcc gaaagccgtc gcctgcgccc gtgccaatat tgcccgtttg ggctttgaaa
    27361 aacaggttga aatccgcgaa accgatctgt ttcccgaagg gtttgccgat ttaatcgtct
    27421 gcaatccgcc ctggcttccc gccaagccga cttccgccgt cgaatccgcg ttatacgacc
    27481 ccgaatctgc gatgctggct gcgtttttgc gggatgcgcc gaaacatctg aatctcgacg
    27541 gagaaatccg cctgatcatt tctgatcttg ccgaacatct gcacctgcgt ccgtccgatt
    27601 ttctggataa ggcatttgct caggcgggtt tgcgcgttgc ggatgttttg aaaaccaaac
    27661 cggttcacaa aaaagctgcc gatccgaaca atccgctggc ttttgcccga aacagggaaa
    27721 ctactttcct gtaccgtttg aaaaaggcat aaggggcggc ggtgcgcttc cggcggcagg
    27781 catcggaacg cccgcgccgc cggcacggca tcattcgggc ggattattct tgtgaaaata
    27841 cccgctcgag catactgccc aatgccgtct gacgcgtttt gacggtggcg gcagcttgcc
    27901 ggaaggcttc ttccccgccg aagaatacga acttctctct cgcgcgggta atggcggtat
    27961 ataacagctc cttactcaat ccggacaatg catcgtcccc ttcgtccgaa ggtgcggcgg
    28021 aaggcggcag cagccatact tcccggtatt ccgaaccttg gcttttgtgg acggtcatgg
    28081 cgaatgcggg ttcaaattcg ggcaggcagc ttaccgctac ctttttaaat ccgtccgcat
    28141 cggcaaaata ggcggcaagg ctgccctgcc gtccgacatc ttccataatc aatccgatgt
    28201 cgccgttgaa cagttcaagc gcgtagtcgt tctgtctgat cataatcggt tctccggcga
    28261 aataggcctg atgttccggt atgttcattt tgcggcgtac atggcggcaa taggcttcgt
    28321 tgaagtcttc cgcatcctgc cgccaagctg ccaggaccac gatatccgag atgcctgcgt
    28381 atgcggcttc gatattgccg tcttttaccg cctgccaata ggctttgtgc gcccggtaca
    28441 acctttcgac tcgagcgttc ggactgcatt ccgaatgttc cagttcgtcc ggaaaccggt
    28501 caaacaatgc ccacgccccc tcatcgcccg atacggcggc acgggcaagg cagccgatgc
    28561 cgctgttgtc gccgaagcgg tggctgaacg acagatgggc ggtgttttgc gccaacacgg
    28621 gcggatttgc gctgacgctg aaaccgtgtt ccggaaggaa gccggccagc ctttggtgcg
    28681 tttctccgtc caaaacggtt ttttgcgaca aaacggacag caccgccccg attccgacgg
    28741 acgggagctg gttttcatcc cccagcagaa tcacgcgcgc gccggttttg accgctttca
    28801 aaagttgcag catcaatgcg gtatccaaca tggaggcttc atcgacaatc agcacatcga
    28861 acggcagcgg acggatatgg tcgaacgccg cctgcatttt gggcgggcgc agcttcagca
    28921 atcggtggac ggtttgccct tccagtttga gcaaatggcg gcggacggcc tccggcgcgt
    28981 caaaaccgtt gattgcacgg tgcagcgcgc gcgccatatg tgctgccgct ttgcccgtcg
    29041 gtgcggcaag cgcgatatgg ggaagatttt cgtcttcacc gcaaatcagc gccagcagtt
    29101 tggcaaccgt tgtcgttttg cccgttcccg gcccgccggt aatcaccata aaagactgta
    29161 acagtgccaa ggcggcggca tcgcgctgtc cttcgctgcc cgcgccttga aaccattttg
    29221 cgaggttttg ccgcgcacct gtcgcgtcgg gggcggatgt gccggctgcc gccaagcgtt
    29281 ttatctcggc agccaaatcg tattccaact gccacatcct gcccaaaaac agccttctgc
    29341 cttccaaaat caaaggcgcg gcggatgttc cgaccacggg tgcgagtgcc gacagcgcat
    29401 cagcctcgcc accgctcaaa cggataaacg aatgaccgtt ctgcaatgcc tgaaacaggc
    29461 gttcggtaca gtttgcaagc acttcgtcgc cagaacccgc atagtgttcc aaaaaacgga
    29521 ttgccgccct tgccgccgct tgggcaaatt catctgtctg ccgttccatc gtattccttg
    29581 tcgaaatgcc gtctgaaggc gtggggcttc agacggcgcg gtgttgtttc ggctgtttag
    29641 gcgtttgcgc cctgtttggg gtgcaggctg ccgtctttca tgaccatcac gcgcccgaag
    29701 cggccggcga gttcgtcgtc gtgcgttacg accaccagcc ccgttcccaa ttctgttttc
    29761 agttccagca tcatatccaa aacattcctt gcgtttgctc ggtcgagatt gccggtcggt
    29821 tcgtcggcaa gcaggcattt gggttgcgta accaaggcgc gcgcaatggc ggcacgctgc
    29881 cgctcaccgc cggacagttc gcccgcgcgg tgcgtcgaac ggtgtttcag tccgaccttt
    29941 tccagcatcg ccattgccgc ctccgcagcc tcttcacggc tttttttgcc gatcagaagc
    30001 ggcatcatca cgttttccag tgccgaaaat tcaggcagaa gatggtggaa ctggtacacg
    30061 aaaccgagat ggcggttacg taaatcgccc aaacgccgct ggtttaaggt acgcaaatcc
    30121 tcgcccatca gcagcaccct gccttcagac ggcatatcca gcccgcccag aatatgcagc
    30181 agcgtcgatt ttccgctgcc cgaagaaccg atgatgccag tgctttcccc cgcgcggatt
    30241 tccaaatcca agccgtgcag cacccgaacg tccaaaccgc cgtcccgata gcgtttgccc
    30301 acgccttcgc atttcaaaat caactcactc ataacgcaaa gcctccgccg gttgggtttt
    30361 tgacgcgcgc cggctcgggt agagcgtggc aacgaaagac agtcccaaag aaatgcaggc
    30421 aatcagggca acgtcgccca tatcgacatc gctgggcagg tagtcgataa aataaacctg
    30481 cgaattgatg aggtggacac cgagcaggtt ttcaaaaaac gccacgaccc tgccgacgtt
    30541 ccaacccaaa agcacgccgc agaccacacc cgccagcgtg ccgaaaaagc ctgaaaacgc
    30601 gccctgcacc ataaaaatct tcatcacgcc gccaggggaa agacccaaag tccgtaaaat
    30661 cgcaatgtcc gcctgctttt ccgtaaccgc catcaccaag gaagagacaa ggttgaacgc
    30721 cgccacggcg ataatcagcg tcaggatgat gaacatcatc cgtttttcca actcgaccgc
    30781 ctcaaaatag ctgcggttgt tgtacgtcca atcgcgcacc caaaccgcgt ccctttgcgc
    30841 ctccggaatc agtgttgccg tcaaggcggg ggcgttttgc gggtcggcga gtttcagccg
    30901 cagccccgca acttccttat ccaagcggta cagtacgcgc gcgtcttgga tatgcgtcat
    30961 cgccaatgag ttgtccactt cgtaaacgcc cgtcttaacc agaccgacca cggtaaactg
    31021 tttcaacctc ggtacgactc cggcgggcgt aacattgccc tccggcgtga tgacggtaac
    31081 tttattgccg acttccgccc ccaaagcctc cgccaagccg acaccgagga taatgtcaaa
    31141 ctcgcccgga atcaggtctt caaatttgcc tgccggcatt ttgtcgccgt attccaccac
    31201 tttgcgctct tcagacggca aaatgccgcg catctgcacg cccctgattt cgcccgcatt
    31261 ggccagcaat gcctgattgg aaacataggg cgcggcagcc aaaatacctt tgcggttttc
    31321 ggtaaatcga agcaggttgc gccaatccgt atccgtattg tcgatatagc cgatttcggc
    31381 gtgcggcgcg acattcagga gctgcccgcg tatttctttc tgaaagccgt tcataaccga
    31441 caagacgaca atcagcgcgg ttacgcccaa ggcgattccg gcaatcgaaa ccatcgtgat
    31501 aaacgacata aagccgttgc gctttttcgc cctgagatac ctcaagccta tccaagcctc
    31561 tagagaaaac ataacgctac cttaaaaatg tctgcaaacg tgccgccccg gacggcggtt
    31621 tgggggcggc ggcaaaagtt tattgtaccg taaaacccag gcagcgtccg aacgcccgag
    31681 tgcgggggac ggggcggcag agcgggcgga aaacttgcac aacgcgtcaa actgccctat
    31741 cctttccttc agaaaaaccg tttcttgagg aaaacaatga atatccgaac tgcttttgct
    31801 ttgtgcgcca tcgccttatc cgccgctgcg gctgcctacg ccaaagaaat caaaatcgat
    31861 gccaacaaca cgccttattc cgaagccgac gcgcaaaagc tggcggcaac ggcagtcggt
    31921 atgggtgtta aggaacctat cagcctgaac ggcggcagcg gcagcattac cgtgtccggc
    31981 agcagcgcga cgcagtgcgt gttcaaagtc ggcaacggag gcgcattgca gattcaaggg
    32041 ctaaactgca agtaaaccgc ccggaaaaat gccgtctgaa ggcttcagac ggcattttgc
    32101 attggcggcg ttatgccccg ccttctttaa tcaggcggcg ttcgtacacc gcctgcgcca
    32161 gcgttcccgc atcgacatat tccaattcgc cgcccaaggg aatgccctgc gacagcctgc
    32221 tgactttgta aggcaggttt ttaaaaaact cggacaggac atacgccgtc gcattgcctt
    32281 ctgcggtaaa agcggttgca ataatgattt cttcgacttc cccgccgccc agccgttgcg
    32341 ccagcctgtc caatgcgatg gcggatacgt ccattcccag tgccgtattg atttgcccca
    32401 tcaggacgaa atacagcccg tcgtggcagt ttgccgcttc catattcgac acgtcggcag
    32461 gcatatgcac caccatcagc cgccgcccgt cgcgtgtttc atcggcacaa atatcgcaca
    32521 atccgccttc gcaaaacgtg ttgcacatcg cgcaatggta aacctgcttc aatgccgtct
    32581 gcaaggcatc caccagtttt tcagcctctt tgcgcttgtg ttggagcaaa tgatacgcta
    32641 tccgctgtgc caatttcggc ccgacgttgg gtaaaacctt cagcgcgtcg atcaatcctt
    32701 ggaaggcatc ttgttttttg tggctcatca tattctgccg tatgggaaaa cgtccggaat
    32761 attccgaccg ttattttgtc aacaaaagtg tcaattactg accgtcgccg ttgtcgaccg
    32821 attgcgctcc tttggtctgt ttgattttgc cgttgaaata acgtatcaac aagtcgaaag
    32881 tattggcaga ctgctgttgc gccaaagcct gttttgcaag cggaagctgt gcggcgatat
    32941 catccggcgg ggttatagcc tgtacttcga caatcacggg tgccggcaga ccggtcagcc
    33001 tgacgtaggc gggtttgccg tttgccggtt ttgctttcag cagttccgca taagcctcgg
    33061 gcggcatgga ctgccttgcc tgctgcgcgc ccaaaacgga cacttccgac catttcacgt
    33121 caactgcctt gcctccgctc aactgcgcca gcacttcttt tgccttgttt tcggcaagtt
    33181 tggcggcttc ggtacggata taagcctgac gcaccgcatc tttggcttcg gcaaacggca
    33241 gggttttctc ttcgcggact tctttggcac ggacgaccca cgcggtttcg ctgttgatgg
    33301 tcagcacttc ggaattgtgt tttttcttca atacgtcgtc gctgaatatg gcattgatca
    33361 ggttttcagg cagtccggac atttgcgcgt cctgcctgct cagccaagtt tcttgggttt
    33421 cgactttcaa actgctgttt ttggcggctt cggcaagcga agcgggatgg ttgaacgcgt
    33481 catcgcccag cttttctttt gccttattga agtcggcaac cgcctttttc attttcaact
    33541 cgttctcaat ggcggctttt tcctgctcga aagaaggttt ggcttcattt gccggtaaac
    33601 gcgccacgcg ctcttcatac acatttttca cttccgtttc gctgacggtc tgtttgtctg
    33661 caaaatcctt cagattcaat gcgacatatt ccaatttgac cgcctgcggc agcagatagt
    33721 cttttttgtt cgcattataa aatttctgca aatcggcttc agacactttg acttgggcga
    33781 tgaactcgtc ggggttgaaa gtgtgcgaac ggatggtgcg gttgacctgc gtcagcctga
    33841 tcagctgttc cgcctgcgcg tcgccgacca atacgccgtt ttggacgagg tttaccaaat
    33901 tctgcaaggc aaactgatcg tggatttctt cgacaaactg gtcttcagac atatggcgtt
    33961 gggaaaggta gcggtttaaa agcgcgtggt cgaatttgcc gtttgcgtcg tggaaattgg
    34021 gatcgtccac gataatctgc ttgatttgtt cggaagaaac cgaaatgccc atcagcttcg
    34081 cgccctgttt caggtaggcg cgttgcagca gggattggaa caccgcgtcg cgcgaagggc
    34141 tgccgccgcc cgcctgttca ttttgcatgg cgttgttgat ggagtggtcg ctgatttttt
    34201 cgtcgcccac ttggacgatg tagtcggcac ccggatggga taccgtgctg accccgaagc
    34261 cgacgaaggt taatgcaatc aggcccaaaa ggacttgggc gggcgttctg tatttttcga
    34321 tggaatggaa catattttaa atcgggatat agaatgggaa cgggaaattc aagtcgggta
    34381 ttgtaacggt ttttatccct gtctgcacgg ggcttgccgc ttgaagatgc cgtcgtaggt
    34441 ttcttcgctg aagccggcgt aaaccttgcc gccgcactcc aatacgggac gcttgatcag
    34501 gctcggcatt tcggacatca atttgacggc ctccgccgtc gaggacagca ctttttgctg
    34561 tgtttcggca tcgagtttgc gccagcttgt cccgcgtttg ttgagcaggg ttgccaaagg
    34621 cacttgttcc agccacgagc agatttccgc ttcagacggc ctctgttttt tgaaatcccg
    34681 aaactcaaac tccaagccgt atccggcaag tcggtttttg gcttttttga ccgtatcgca
    34741 atttgggatg ccgtgaagga ctatcatttg gaaacctttt gtctgaaata ataaaacgga
    34801 tattttacta taagtgtctg aaaatttgcc cgtctgtttc agacggcggg gcggttatgt
    34861 tacaatccga aaattcgaaa aatttaatct cttgttcaat aaaggcttta ccaatcatga
    34921 tttctaccaa cggcatcacc atgcagttcg gcgcgaagcc gctatttgaa aacgtatccg
    34981 tcaaattcgg cgaaggcaac cgatacggtt tgatcggcgc gaacggctca ggcaaatcta
    35041 ctttcatgaa aatcctcggc ggcgatttgg aacagacggc cggcgaagtg gcgattgaaa
    35101 acggcgtgcg tttgggtaaa ttgcgccaag accagtttgc ctacgaagat atgcgcgtgc
    35161 tggacgtggt aatgatgggt cacaccgaaa tgtgggcggc gatgaccgaa cgtgatgcga
    35221 tttacgccaa tcccgaagcc accgaagacg actacatgaa agccgcagaa ctggaagcca
    35281 agttcgccga atacgacggc tacaccgccg aagcgcgtgc cgccgagttg ttgagcggcg
    35341 tgggcatttc cgaagatttg cacaatgcaa ccatggcaga agttgccccg ggcttcaaac
    35401 tgcgcgtatt gctggcgcaa gccctgttct ccaagccgga tgtattgctc ttggacgaac
    35461 cgaccaataa cttggacatt aataccatcc gttggttgga aggcgtgttg aaccagtacg
    35521 actccacgat gattatcatc tcgcacgacc gccacttctt gaacgaagtc tgcacgcata
    35581 tggcagattt ggattacaac accatcacca tctatccggg caactacgac gactatatgc
    35641 tcgcctccgc ccaatcgcgc gaacgcgccc tgaaagacaa tgccaaggcg aaggaaaaat
    35701 tgcaggaatt gcaagaattt gtggcgcggt tcagtgccaa caaatccaaa gcccgtcagg
    35761 caaccagccg tctgaaacag gcagacaaaa tcaaatcgga gatggtcgag gtcaaaccct
    35821 ctacccgtca aaacccgtat atccgttttg aagccgatga aaaagccaag ctgcaccgtc
    35881 aggctgtgga agttgaaaaa ctggcgaaac gctttgaaac ccagttgttt aaaaacctga
    35941 acttcatcct tgaagcggga caacgcctcg ccatcatcgg cccgaacggc gcgggcaaat
    36001 ccaccctgct gaaactcttg gccggcgcgt acaaccccga atattcagac ggtctgctgc
    36061 cggacgaagg cagcatcaaa tgggcggaaa aagccagcgt cggctactat ccgcaagatc
    36121 acgaaaacga cttcgatgtc gatatggatt tgagcgaatg gatgcgccaa tgggggcagg
    36181 acggcgacga cgaacaagtc atccgcggca ctttggggcg tttgctcttc ggcagtaacg
    36241 atgtcgtgaa aaaagtgaag gttctctccg gcggcgaaaa aggccgtatg ctttacggca
    36301 aactgttgct gttgaaaccc aatgtcttag tcatggatga accgaccaac cacatggaca
    36361 tggaaagcat cgaatccttg aacatggcgc tggaaaaata caacggcacg ctgattttcg
    36421 tctcccacga ccgtcagttc gtttcttccc tggcaaccca aatcatcgaa ctggacggca
    36481 aaggcggata tgaacactac ttgggcgatt acgaaagtta cttggagaaa aaaggcgtag
    36541 cataaccgcc ggttggaaca atgccgtctg aagccgcttc agacggcatt gttgataact
    36601 ttaaaatagg aagcatatgc agacttacct cgtcggcggc gccgtccgcg attatctttt
    36661 gggcttgccc atcaaagacc gcgattgggt ggtcgtcggc gcagacgcac aaaccatgct
    36721 ggcgcaaggc ttccagcctg tcggcaaaga ttttcccgtg tttctccatc ccgaaacaca
    36781 cgaagaatac gccctcgccc gcaccgagcg caaaaccgcc aaaggttacg ccggcttcag
    36841 tttccacacc gacaaagacg ttacgctgga gcaggatttg atgcgccgcg acctgaccat
    36901 caacgcgatg gcgcaagatg cggacggcaa gattatcgac cctttcggcg gacaacggga
    36961 tttggcggca ggcattttgc gccacgtttc cccggccttc gccgaagacc ccgtccgcat
    37021 cctgcgtact gcccgctttg ccgcgcgtta caggtttgaa atcgccgaag aaaccataaa
    37081 gctgatgcgg cagatggtgg aaaacggcga agcggacgca ttggttgccg aacgcgtctg
    37141 gcaggagttt gcgaaaggtt tgatggaaaa aaatccgcgc aaaatgattg aagtgttgcg
    37201 cgaatgcggc gcgctcaaag tcttgctgcc cgaagtcaat gccctcttcg gcgtgccgca
    37261 acgcgccgac taccatcccg aaatcgacag cggcatccat accctgatga cgctgcaacg
    37321 cgccgccgat atgggtttga gcctgcccga acgctatgcc gccctgctgc acgacttggg
    37381 caaagccaaa acaccgtccg acatcctgcc gcgccaccac gggcacgacc tcgccggtgt
    37441 cgaacccgtg cgcgaagtca atcagcggct gcgcgcgccg aaacattgcg ccgagcttgc
    37501 cgaattggtt tgccgttggc acattatttt ccaccaagtc ggacagctta aaagccaaac
    37561 cattctgaac gtattgaaaa aaaccgatgc cttccgccgt cccgaacgct ttcagacggc
    37621 attgaacgtc tgcattgccg acacgcaagg ccgtctgaac cgcgaacaca cgccctaccc
    37681 gcaacgcgca cactggctcg ccctgctcga agccgccaat caggcggatt cgggcaaaat
    37741 cgccgccgaa tgctgggcgc agggaaaagc gcaccttatc gccgaacaaa tcgaccgggc
    37801 gcggctggcg caaatcgccc cgctgcaaaa agcgtttcga gcggcgcaag acaaaacaga
    37861 aaaacattaa aacgtccaat gcagccactt ttatagtgga ttaacaaaaa tcaggacaag
    37921 gcgacgaagc cgcagacagt acagatagta cggaaccgat tcacttggtg cttcagcacc
    37981 ttagagaatc gttctctttg agctaaggcg aggcaacgct gtactggttt ttgttaatcc
    38041 actataaagt tttgaggacg atacccaatc caagctttgc aacagccgcc gccatatccg
    38101 ctataattca cgcttcagcc attccgcccc cgacataaaa tcatgaccct gaaaaccgat
    38161 ttattgctta aaatcaacaa cgaagattat caacgcctca tcctcaaaca catctcggaa
    38221 ttcagcgagg gcgaaatccg cctgttgaac gaaatccttg aaaaattcaa tttcgacgtc
    38281 gttcaggcgc aggcattggc gcaggcggta atgcagcaag tccgcttcga ccccaacgcc
    38341 taccacatcg acagcgacga cgaagacacc acaggcatct gcccccactg catcaacccg
    38401 cccatgccgc ccctgcgcga ttacctcgtt tggcgcgaaa cgcgcggata aaatgctttt
    38461 gaccgttatc ttttcaatgc cgtctgaaac gccgccgacc gttcggacgg catacccgac
    38521 aaagggaaca ctatgctgca aaccgacaac ctgaccgccg cgcaaccgca acgcatcgtt
    38581 gccgcccaaa ccgcctccgc acaggaagaa ctgctcgaac gcgccctccg ccccaaaacg
    38641 ctggacgact acatcgggca agacaaagcc aaagaacagc ttgccatttt catccaagcc
    38701 gccaaaaaac gcggcgaagc actcgaccac gttttgctct tcggcccgcc cggactgggc
    38761 aaaaccacac tggcgcacat catcgccaaa gaattgggcg taaatttgcg ccaaaccagc
    38821 ggccccgtcc tcgaacgcgc aggcgacctc gccgcccttt tgaccaacct tgatccgcac
    38881 gatgtattgt tcatcgacga aatccaccgc ctcagccctg ttgtcgaaga aatcctctat
    38941 cccgcgctcg aagactaccg gctcgacatt atgataggcg aaggacccgc cgcccgttcc
    39001 gtcaaaatcg acctgccgcc cttcacgctc atcggcgcga ccacccgcgc cggtatgctg
    39061 accaatccgt tgcgcgaccg cttcggcatc gtctcccgcc ttgagtttta cgaaaaccga
    39121 gaccttacca ccatcgtcag ccgttcggca caactgttgc agctcgatat gtccgaagaa
    39181 ggcgcggaag aaatcgccaa acgcagccgc ggtacgccgc gcatcgccaa ccgcctgttg
    39241 cgacgcgtgc gcgatttcgc cgacgtgaaa aacaacggca caatcgacgg cggcatcgcc
    39301 gatgccgctt taagtatgct ggacgtggac gcgcaggggc tggacgtgat ggacaggaaa
    39361 tttctcgaag ccgttttgca caaattcggc ggcggcccgg tcggtttgga caatgttgcc
    39421 gccgccatcg gcgaatctac agacaccatc gaagacgtta tcgaacccta tctcatccaa
    39481 caaggcttcc tgcaacgcac cccgcgcggc aggatggcga ccgaacgcgc ctacctgcat
    39541 ttcgggctgc ccgtcgaaaa ataacgcaat gccgtctgaa acagagctaa ttttcagacg
    39601 gcatttctat ttcaatcatt ggcgcaaggt tcagcctgcc gcttttttcc agttccgccc
    39661 tcatcgcatc aatcaccgcc ttatagtctg gtttgccgaa aatcgcagaa ccggcaacaa
    39721 aggtatccgc accagctcgg gcaacggcgg caatattgtc ggttttgatg ccgccgtcca
    39781 cttcgatggc gatgtgccgc ccgctttgtg cttcgtaccg atccagcatc gcccgcaccc
    39841 ggcggatttt ttcaagggtg tgcgggatga agctttgtcc gccgaatccg gggttgaccg
    39901 acatcagcaa aaccatatcc agcctgtcca atacgttttc caacagatat acgggcgttg
    39961 ccggattcaa caccagcccc gcctgacagc ccatgtcccg aatcaggctc aagctgcggt
    40021 cgatatggcg gctcgcttcg ggatggaacg tgatgattga tgctcctgct ttggcaaacg
    40081 actgaatcag gtcgtcaacg ggttcgacca tcagatgcac atcgatcggc acgcttgcat
    40141 aaggcttcaa cgccgcgcaa accatagggc cgaaggtcag gttcggcaca taatggttgt
    40201 ccatcacgtc aaaatggatc aaatctgcac ctgccgcaat gacgctttcc acctcttctc
    40261 cgaggcgggc aaagtctgcc gataaaatgc tgggtgcgat acggtaagta gtcatgtttt
    40321 tttccttcaa tat
//
LOCUS       DAWFJW010000004        39574 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFJW010000004 DAWFJW010000000
VERSION     DAWFJW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.004"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..422
                     /locus_tag="WGR19_000139"
     CDS             <1..422
                     /locus_tag="WGR19_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ0797746.1"
                     /translation="LFAESGKTQTVRNTVVKPKPLKAYNLTVADWHTYFVKSSQAETE
                     GVWVHNDCSSRKTPSTPIYEDDSEAYAAAKKLGYRKIKERTRNDAAIFKKGKSYISRD
                     VDSHNGGAWKEASSPKNLNRKETRNGTFDKNLNRIGD"
     gene            428..826
                     /locus_tag="WGR19_000140"
     CDS             428..826
                     /locus_tag="WGR19_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0797747.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            857..1234
                     /locus_tag="WGR19_000141"
     CDS             857..1234
                     /locus_tag="WGR19_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0797748.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            1270..1368
                     /locus_tag="WGR19_000142"
                     /pseudo
     CDS             1270..1368
                     /locus_tag="WGR19_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1639..2772
                     /locus_tag="WGR19_000143"
     CDS             1639..2772
                     /locus_tag="WGR19_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797749.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIMKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQDFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKRGDRLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(2874..3149)
                     /locus_tag="WGR19_000144"
     CDS             complement(2874..3149)
                     /locus_tag="WGR19_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797750.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(3166..3933)
                     /locus_tag="WGR19_000145"
     CDS             complement(3166..3933)
                     /locus_tag="WGR19_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0797751.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCKPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQNNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPE
                     YEAYEDKRSIPENPYIHELQYIKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTVQ
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            4377..4727
                     /locus_tag="WGR19_000146"
     CDS             4377..4727
                     /locus_tag="WGR19_000146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797752.1"
                     /translation="MPSETKFRRHQAIKSNPASSSSDKPHPHHRFADKYRQPASTALH
                     HNHTAAYHPETPQDNNCRQPNSKTVRYREQAIRLATVGNPIVHKPVVKRFGIRFPVEE
                     ILRHLGKNRSFHCR"
     gene            complement(4877..5152)
                     /locus_tag="WGR19_000147"
     CDS             complement(4877..5152)
                     /locus_tag="WGR19_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0797753.1"
                     /translation="MNALNWSLYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(5149..6432)
                     /locus_tag="WGR19_000148"
     CDS             complement(5149..6432)
                     /locus_tag="WGR19_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0797754.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(6580..7998)
                     /gene="glnA"
                     /locus_tag="WGR19_000149"
     CDS             complement(6580..7998)
                     /gene="glnA"
                     /locus_tag="WGR19_000149"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0797755.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDTTVVFTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(8272..8649)
                     /locus_tag="WGR19_000150"
     CDS             complement(8272..8649)
                     /locus_tag="WGR19_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797756.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            8820..9629
                     /gene="aroE"
                     /locus_tag="WGR19_000151"
     CDS             8820..9629
                     /gene="aroE"
                     /locus_tag="WGR19_000151"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0797757.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGLGLVGDIVKVQHTEIEGKNILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELSRLFGIEAVPMADLNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            9631..10332
                     /gene="mtgA"
                     /locus_tag="WGR19_000152"
     CDS             9631..10332
                     /gene="mtgA"
                     /locus_tag="WGR19_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0797758.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDAHFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(10534..10609)
                     /locus_tag="WGR19_000153"
     tRNA            complement(10534..10609)
                     /locus_tag="WGR19_000153"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10574..10576),aa:Phe,seq:gaa)
     gene            complement(10800..11534)
                     /gene="lptB"
                     /locus_tag="WGR19_000154"
     CDS             complement(10800..11534)
                     /gene="lptB"
                     /locus_tag="WGR19_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0797759.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(11608..12138)
                     /gene="lptA"
                     /locus_tag="WGR19_000155"
     CDS             complement(11608..12138)
                     /gene="lptA"
                     /locus_tag="WGR19_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0797760.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQGDSRQPIQIEADQGSLNQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRNDKGEQFMKADGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(12119..12700)
                     /gene="lptC"
                     /locus_tag="WGR19_000156"
     CDS             complement(12119..12700)
                     /gene="lptC"
                     /locus_tag="WGR19_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0797761.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVKTEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGLLNFSSKVKATIYDTKDM"
     gene            complement(12697..13233)
                     /locus_tag="WGR19_000157"
     CDS             complement(12697..13233)
                     /locus_tag="WGR19_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0797762.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRVQA
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(13406..14380)
                     /locus_tag="WGR19_000158"
     CDS             complement(13406..14380)
                     /locus_tag="WGR19_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0797763.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            14476..15531
                     /gene="tal"
                     /locus_tag="WGR19_000159"
     CDS             14476..15531
                     /gene="tal"
                     /locus_tag="WGR19_000159"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0797764.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEVRRLYAAIGCKNAMIKVPATDAGIDALETIVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDYLK
                     GKIAIALAKAAYQDWAQYFGSSEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            15685..15975
                     /locus_tag="WGR19_000160"
     CDS             15685..15975
                     /locus_tag="WGR19_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797765.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            15992..16879
                     /gene="gluQRS"
                     /locus_tag="WGR19_000161"
     CDS             15992..16879
                     /gene="gluQRS"
                     /locus_tag="WGR19_000161"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0797766.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGRWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWYGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(16947..17957)
                     /gene="dusA"
                     /locus_tag="WGR19_000162"
     CDS             complement(16947..17957)
                     /gene="dusA"
                     /locus_tag="WGR19_000162"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0797767.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEANLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDAMLLKDNDGGLILEAWEEVERANT
                     RE"
     gene            complement(17988..18479)
                     /locus_tag="WGR19_000163"
     CDS             complement(17988..18479)
                     /locus_tag="WGR19_000163"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0797768.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(18553..19311)
                     /locus_tag="WGR19_000164"
     CDS             complement(18553..19311)
                     /locus_tag="WGR19_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0797769.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(19382..20248)
                     /locus_tag="WGR19_000165"
     CDS             complement(19382..20248)
                     /locus_tag="WGR19_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0797770.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(20297..20575)
                     /locus_tag="WGR19_000166"
     CDS             complement(20297..20575)
                     /locus_tag="WGR19_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0797771.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(20575..20865)
                     /locus_tag="WGR19_000167"
     CDS             complement(20575..20865)
                     /locus_tag="WGR19_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0797772.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(20868..21398)
                     /locus_tag="WGR19_000168"
     CDS             complement(20868..21398)
                     /locus_tag="WGR19_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0797773.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            21706..24219
                     /locus_tag="WGR19_000169"
     CDS             21706..24219
                     /locus_tag="WGR19_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0797774.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSAQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTKTVAEIPD
                     FNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLETPDS
                     NTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKYDLAE
                     MYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(24391..24807)
                     /gene="gloA"
                     /locus_tag="WGR19_000170"
     CDS             complement(24391..24807)
                     /gene="gloA"
                     /locus_tag="WGR19_000170"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0797775.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAIEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVVYANT"
     gene            complement(24854..26023)
                     /gene="lapB"
                     /locus_tag="WGR19_000171"
     CDS             complement(24854..26023)
                     /gene="lapB"
                     /locus_tag="WGR19_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0797776.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHNDQTYQFEIAQFYCELAQASLFKSNFDVARFNVGKALEANKRCTR
                     ANMILGDIEYRQGNFPASVEAYAAIEQQNHAYLSMVGEKLYDAYAAQGKLEEGLNRLI
                     GYMQTFPELDLINVVYEKSLLLREEKEAAQTAIELVRRKPDLNGVYRLLGLRISDMDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(26034..26348)
                     /locus_tag="WGR19_000172"
     CDS             complement(26034..26348)
                     /locus_tag="WGR19_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0797777.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQELTAPPAQNAAEPAK
                     QP"
     gene            26658..27656
                     /gene="ilvE"
                     /locus_tag="WGR19_000173"
     CDS             26658..27656
                     /gene="ilvE"
                     /locus_tag="WGR19_000173"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0797778.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(27900..28685)
                     /gene="fabI"
                     /locus_tag="WGR19_000174"
     CDS             complement(27900..28685)
                     /gene="fabI"
                     /locus_tag="WGR19_000174"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0797779.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGADLAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(28840..29661)
                     /gene="dapD"
                     /locus_tag="WGR19_000175"
     CDS             complement(28840..29661)
                     /gene="dapD"
                     /locus_tag="WGR19_000175"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0797780.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(29871..31514)
                     /gene="pgi"
                     /locus_tag="WGR19_000176"
     CDS             complement(29871..31514)
                     /gene="pgi"
                     /locus_tag="WGR19_000176"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0797781.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPVTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            32024..33256
                     /gene="pilG"
                     /locus_tag="WGR19_000177"
     CDS             32024..33256
                     /gene="pilG"
                     /locus_tag="WGR19_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0797782.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            33330..34190
                     /locus_tag="WGR19_000178"
     CDS             33330..34190
                     /locus_tag="WGR19_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0797783.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRARQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            34192..34824
                     /locus_tag="WGR19_000179"
     CDS             34192..34824
                     /locus_tag="WGR19_000179"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0797784.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFIILIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            34817..35026
                     /locus_tag="WGR19_000180"
     CDS             34817..35026
                     /locus_tag="WGR19_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0797785.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(35111..36787)
                     /gene="pilB"
                     /locus_tag="WGR19_000181"
     CDS             complement(35111..36787)
                     /gene="pilB"
                     /locus_tag="WGR19_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0797786.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(37187..37258)
                     /locus_tag="WGR19_000182"
     tRNA            complement(37187..37258)
                     /locus_tag="WGR19_000182"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37223..37225),aa:Arg,seq:cct)
     gene            complement(37278..37724)
                     /locus_tag="WGR19_000183"
     CDS             complement(37278..37724)
                     /locus_tag="WGR19_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0797787.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(37729..38703)
                     /gene="ispB"
                     /locus_tag="WGR19_000184"
     CDS             complement(37729..38703)
                     /gene="ispB"
                     /locus_tag="WGR19_000184"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0797788.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(38719..38907)
                     /locus_tag="WGR19_000185"
     CDS             complement(38719..38907)
                     /locus_tag="WGR19_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797789.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            38927..39235
                     /gene="rplU"
                     /locus_tag="WGR19_000186"
     CDS             38927..39235
                     /gene="rplU"
                     /locus_tag="WGR19_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0797790.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            39261..39533
                     /gene="rpmA"
                     /locus_tag="WGR19_000187"
     CDS             39261..39533
                     /gene="rpmA"
                     /locus_tag="WGR19_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0797791.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT         9814 a        10688 c         9837 g         9235 t
ORIGIN      
        1 ggctgtttgc cgaaagcggc aaaacccaaa ccgtccgcaa caccgttgtc aaaccaaaac
       61 cgctcaaagc ctacaatctg accgttgccg attggcatac ttacttcgtc aagagcagtc
      121 aggcggaaac ggaaggggtt tgggttcata atgattgctc gtcaagaaaa acaccatcta
      181 cccccatata tgaggatgat tcagaagcat atgcagcagc aaagaaattg ggatatagga
      241 aaataaaaga aagaacgaga aatgatgctg ctatttttaa aaaaggaaag agttatatat
      301 cacgcgatgt agattcacat aatggagggg cttggaaaga agcatcttca cccaaaaatc
      361 tgaacagaaa agaaacaaga aacggtacat ttgataaaaa cctaaatcgt attggagatt
      421 aattattatg tgcgagttca aggattttag aagaaacatc ccttgttttg aagagtatga
      481 cgaaaattca tttattggca aatggtatga tgacggggtt tgggatgatg aagaatattg
      541 gaaattggag aatgctttaa tcgaggttag aaaaaaatat ccttatccga tggatatacc
      601 aagggacatc gtgattggaa tcggtaccat tattgatttt ttaatggttc aaaattggaa
      661 actttttgaa attaaagctt ctccttggtt gcctaaaagt gtcaaaatta atgagcgtta
      721 tgaaaggttc agagtaatgc tccgttatat ttttactgat ctagatgtag aggactggaa
      781 atttttttac tttccaatac aacatagtaa aggtagattg agatgaaaag tttaattata
      841 ttggagatac attattatgt gtgagttcaa ggatattata agaaacgttc cttattttga
      901 ggggtatgac gaaaattcat ttattggcaa atggtatgat gacggggtgt gggatgatga
      961 agaatattgg aaattggaga atgatttaat cgaggttaga aaaaaatatc cttatccgat
     1021 ggatatacca agggatattg tgattggaat cagtaccatt attgattttt taatggttcc
     1081 aaattgggag cttttcgaaa ttaaagcttc cccttggcta cctgatagcg tgggaattca
     1141 tgaacgttat gaaagattca caacaatgct ccgttatatt tttaccgaga aagacatagt
     1201 caacgtgcga tttgattatt acaacaaaaa atagcaaacc ccggcaatca aaatgccgtc
     1261 tgaagcggta ttcggctttc agacggcatc ggtaacagcc aaaccctgat ttccaaccgc
     1321 atccatccgt tttattcgga cggcaaatgg attaaggcag aagacttaat attggtggga
     1381 aatggaaacg ttcaaaatat cagttttaga agattgggaa cgctcgggaa gaccgttaaa
     1441 ttgggaaaaa tggcaaaggc aatatcgatc ttaagcattt gaattagttg aaaatttgaa
     1501 tcaaagcaaa caagagtaac aagccgcagc ctgcataggt ttttttaaag tacggcagca
     1561 agcgtagatt agattaaaaa tcaaacaatc aaagctcgtc tgatataatc gatatattct
     1621 gttaaacgaa aaataaatat gtttaaaaaa ttcaaaccgg tactgttgtc attttttgca
     1681 cttgtatttg ccttttggct gggaacgggt attgcctatg agattaatcc gcgttggttt
     1741 ttgagcgata cggcaactga aatacctgaa aatccgaatg cttttgtggc gaaacttgcc
     1801 cgcctgttcc gaaatgccga cagggcggtt gtcatcatga aggaatcgat gaggacggag
     1861 gaaagtcttg ccggagctgt ggatgacggt ccgttgcagt cggagaagga ttatctcgcg
     1921 ctcgctatcc ggctcagtcg tttgaaagaa aaggcgaaat ggtttcacgt aacggagcag
     1981 gaacatggga aagaggtttg gctggattac catatcggcg agggcggttt ggttgcggtt
     2041 tcgctttcgc aacgctcgcc ggaagcgttt gttaatgccg aatatctgta tcggaacgat
     2101 cgtccgtttt ctgtaaatgt gtacggcgga acggctcacg gagaaaatta tgaaacgaca
     2161 ggagaatatc gggttgtttg gcaaccagac ggttcggtat ttgatgcgtc ggggcgcgga
     2221 aaaatcgggg aagatgttta tgagcattgt ctcgggtgtt atcagatggc ccaggtatat
     2281 ttggcgaaat atcgggatgt cgcgaatgat gagcagaagg tttgggactt ccgcgaagag
     2341 agcaaccgga ttgcgtcgga ctcgcgtgat tatgtgtttt atcagaatat gcgggaattg
     2401 atgccccggg ggatgaaggc aaacagtctt gtggtcggct atgatgcgga cggtctgccg
     2461 cagaaagtct attggagttt cgacaatggg aaaaaacgcc aggatttcga atattatttg
     2521 aaaaacggaa atctttttat tgcacaatct tcgacggtag cattgaaagc ggatggagta
     2581 acggcggata tacagaccta tcatgcgcaa cagacgtggt atttggatgg cgggcggatt
     2641 atccgcgaag agaaacgggg agacagactg cctgattttc ctttgaactt ggaaaatttg
     2701 gaaaaagagg tgagccgtta tgcagaggct gcggcgagac gttcgggcgg caggcgcgac
     2761 ctttctcact gaacgcaaat gccgtctgaa accggttttc agacggcatt ttttcagacg
     2821 gcactggaga gtcaaacgct ttttgcaatg ccgtatattc caaatccaat tcgctataaa
     2881 ttgaaaacca caatccaaaa aacggaaata cacattaaac ggatcttttt accgaccgta
     2941 tcatcccata ggttttccac accttttttc atccataaat tcaaaccatt taagtttgca
     3001 tccttaccga caattgcagc agcatcatcc gcataacggt cacccaaatt agccaaataa
     3061 cgccaaccgc cataggtatc gccacggaaa gcataaccac gcaaggtttg gatttgttct
     3121 ggtgttaaaa caacttcacc ctcattcata ataagatcct ttaaattatt ttttacttga
     3181 attgtttaca aaactctgta ccaattgctc tacttgttgg tgataaccta ccaactcctg
     3241 ctgtgtgagc tgctgctttt cccgaataaa cgggtaatac tgtaccgtga aaccgttaat
     3301 acaggaacct atatcagtac tgtaatcgtt ctctccatac ctatgatagt tctgatgagt
     3361 aataatcgcc ggattttctc ctttcttaat atactgtagt tcatgaatat atgggttttc
     3421 aggaatcgac cttttatctt cataagcttc atattcagga tagacagacg ggattttttt
     3481 aaatcccaaa tcaagcaacg attttcctaa gtcttgattc aaatgacacg catcttttcg
     3541 gataacaata tcgtccacgc tatccaaacc atcgcgtaga aattgaataa agtgtgcttt
     3601 gttctgctgg ttgtatggtt cgagcaccca gccttggtcg gtttttttgt aatagacgct
     3661 aatctctatc aaattaatgg agtaagcatc ctgcccgggc gtgtcggaaa gccattttgt
     3721 ggcattgttt tgcgtatacc gtgcaagata gccatagggg ataaccattt tggtgccctg
     3781 aaaatcaaat gtaaccgtat gttcaaatcc tgtcattggc tcgggattgt tgaaactggt
     3841 ttgttcatta acaggtttac atgaggtcat agtaaaaaca ctcccaataa acaaaataaa
     3901 aagttgataa atggtagtag catatggtct cataatttca agcttagaaa ttagttaaag
     3961 aatatattca tggtagatac taactcttta agttgacata ttgtgcgata tcgagggctg
     4021 taccatattt tcccatcggg tcttccccgt tctttatatg agcggcaggc atcctcataa
     4081 aagaagggat accgataagg aaaaatacct cggcataaaa aatatactga ttaatcaata
     4141 caaatccaac aaacagttta tcattttctt ttctgttggc gtataaaaac cgcttggaaa
     4201 aaataaaata tcaaaacggc aaaataaaaa aactgatata tgccttcact ttcatagggc
     4261 gacgctttat gtgccgtcct gtgtgttgaa acatcggttt tcctttttca aaactccgta
     4321 aaaaaagcgg catccgccgc aaaacgcccc gtttttccaa acaaagggca ataaaaatgc
     4381 cgtctgaaac caagttcaga cggcatcaag ccatcaaatc aaaccctgcc tcctcatcat
     4441 ccgataagcc tcatccacat catcggtttg cagataaata ccgacaaccc gcttcaactg
     4501 ctcttcatca taatcatacg gctgcatatc atcccgaaac tccacaggac aataactgtc
     4561 gacagccaaa ctcaaagact gttcgatata gggaacaggc catccggctt gctactgttg
     4621 gcaatccaat cgtccataaa ccggttgtaa aacgtttcgg catccgtttt cccgtcgaag
     4681 aaatcttgcg ccatcttgga aaaaaccgtt cttttcattg cagataatcc gtattcaaat
     4741 aatgctcgcg ctgttttcca cttatcttca ttgccgttac aaacctattg tctttatcaa
     4801 aaacgacagc gtcgcctgcc ctgctttgtc ctgattgttg ccaactgctg taaccatgcc
     4861 gtccgaacct gaaccgtcag gtttcagacg gcattttttc tgcctgctcc cacaattgcc
     4921 gtttttgtgc ggcggtcagt tttttgacgg cgatttcctg cctgagtgcc gtgcctttat
     4981 ccatcccgcc tgcaacgata cgcatcgcca ccggtttgaa tacgcgggta tatttcgcgc
     5041 ctttaccggt tgtgtgggcg gcaagccgct gttgcggatt cgggctgatg ccgcaataga
     5101 acgcgctgtt ttcacacaat atcaggtaaa ggctccaatt taacgcgttc atctgcctgc
     5161 atcctgagtt ttctccccgt tccaaggcgc aactttcagc agcagcagca tacccggtag
     5221 ggcgagtgcg aaacacagtt ggaaaaacgg tacataaccg agccattcaa tcagataacc
     5281 ggcaaaggaa ttgatgaccg tacggggaac agcggacagg ctggtaaaca gcgcaagctg
     5341 cgttgcggta aatgcgggat tggtctcgcg cgccatatac gatacgaacg ccgccgtccc
     5401 caaccccacg ccgaccgctt ccgcgccgat aactgccgcc agcatcagcc tctcgcctgt
     5461 gccgaccgtg tcgaaatgtc cgaaccctgc cagccataca aaccccaaaa cggttacagc
     5521 ctgcaccgcg ccgaatagcc acaaggcttt gtttacgccg attttcagca tccacacgcc
     5581 gcccaagata cctgccgcca ctgccggcca cagtcctgca tttttcgcaa tcaaaccgat
     5641 gtctgtcttg ctgaaaccca tatccagata aaacggcgtt gccaacgcgg ttgccatact
     5701 gtcgccgagt ttgtaaagga agataaacag cagcacgcat acagccgaag cgatgccttt
     5761 acgtgtaaaa aattctttaa acggctctac cacggtctgc ttcaacgttt taggaacggc
     5821 aggcggcaac acgggttcgc gcgcgagaaa cagcgtcatc agaaggccgg gcagcataaa
     5881 taatgaagta ataacaaata cttctgacca cggcatcctg tctgccaaca ccaaactcaa
     5941 tgaaccggga atcagggcgg caatccggta ggcgttcaca tgaaccgagt tgcccaaacc
     6001 caattcttcg tccgacaaaa tctcgcgcct gaacgcatcc aatacaatat cctgactggc
     6061 ggaaaaaaaa gcgacaagca ccgacaagcc ggcaatcagc ggcagatgat tacgggggtt
     6121 taaaaaggca taggccgcca aagccgccag caaccctgcc tgcgtcagca gcatccaccc
     6181 gcgccgccgt cccaaaacgg gcagcctgac cgcgtccatc agcggcgacc acaaaaattt
     6241 ccaagtaaac ggcagaccga ttaacgccat cagcccgatg ctcttcaaat ccacctgctc
     6301 gctgcgcaac cacgccggaa tcaggttaat cagaaagtac agcggcagcc ccgaggtaaa
     6361 ccccgtaaaa atacagacga gcatattgcg ggaaaagatt tgcccgataa aacctgattt
     6421 cgatgcagtc atcgttcgtg cagccaaaaa acaaaagcct attgtagcaa atatcgcccg
     6481 cctgctgaaa aacgccgcgc cgccgttttg ccgttccaat gcaaaatgcc gtctgaaact
     6541 gtgttcagac ggcattgctt ttctgaaaac ccgacgtgct tacaggctgt aatacatttc
     6601 aaattccagc ggatgcggcg ccatgcggat gcggcggaca tcttcctctt taaaggcgat
     6661 gtagctgtcg atccagtctt tgctgaacac gccgccgcgc agcaggaatt cgtggtcggc
     6721 tttgagggcg gcgagtgctt cttccaaaga agcgcaaacg gtcgggacga gcgcgtcttc
     6781 ttccggcggc aggtcgtaga ggtttttatc ggcaggatcg cccggatgga ttttgttttg
     6841 aataccgtcc aaaccggcca tcaacaaagc ggcaaatgcc aagtatgggt tggcggtcgg
     6901 atcggggaaa cgcgcttcga tgcggcgcgc cttgctgctg ttcacggacg gaatgcggat
     6961 ggaagcggaa cggtttttgg cggagtatgc cagtttggtc ggcgcttcaa agtgcggcac
     7021 gaggcgtttg taggagttgg tggacggatt ggtaatcgcg ttcaaggctt tggcgtgttt
     7081 gatgatgccg ccgatgtagt agagcgcggt gtcgctcaag ccggcatagc cgtcgcctgc
     7141 gaacaggttt tgaccgtctt tccaaatgga ttggtgaacg tgcataccgc tgccgttgtc
     7201 gcccataatc ggtttgggca taaaggtggc ggttttgccg aagttgtggg caacgttttg
     7261 aatcacatat ttcatgtctt gggtttggtc ggcgcgtttg accaaggtgg cgaagcgcgt
     7321 gccgatttcc atttggctgc cggtgccgac ttcgctgtgg tggacttcga cttcgatgcc
     7381 gagttcttcc aaaatgttca ccatggcgga gcgcaaatct tggccgcaat caatcggcgc
     7441 gacgggcgcg taaccgcctt taacggcggg gcggtggccg gtgttttgac cgtccatatg
     7501 cagaccgctt gcccatgcgc cgctttcgga cgtaatttcg taacgggttt tgtgcatatc
     7561 ggtttcaaat tctacgccgt caaaaacgaa gaattcaggc tcagggccga aataggcggt
     7621 gtcgccgata ccggaagatt tcaaataggc ttcggcgcgg cgtgcgatag agcgggggtc
     7681 gcggtcatag ccttggccgt cagccgggtc gataacgtcg caggtaaaga caacggtggt
     7741 atcgtcgtag aaaggatcga cgaaggctgt agacgcatcg ggacgcaact gcatatctga
     7801 agcctgaata cctttccaac cgccgatgga cgaaccgtca aacgcctgtc cgttttcaaa
     7861 ccactcttcg gggtcttcca acacgatgcg cgcaggcacg gtaaagtggt gctgcttgcc
     7921 tttggtatcg gtaaagcgca aatcgacaaa gcgggcttcg ctttcttcaa tcaattttac
     7981 ggcgttttta atagacattt ttcagctcct ggaaaaggga aatagcaaaa tatgcggaac
     8041 gcggcagatt agctcaaagc cagcatgatt ctaccacgtc catgccgtct gaaactggcc
     8101 ctgcgtcaaa ttccaagctg taaaatatag tttcagacgg caatattgtc aacaatatgc
     8161 ttcggtttgc ccgttcccaa gcagaatgcc cgccctttta tccgaatgcc gacaaaacag
     8221 atgccgtctg aaagcagttc agacggcatc tgttgtttgg tttgaaaacg gtcagcgagg
     8281 atagccgttg cagacaaatt tgtactgagc ctctccaagc gtaccgggaa taacataatt
     8341 ccattcggca taggatggtg tataagaagc gatagagttt cccgatttgg aataccacgt
     8401 tgccgtcaat atcctaaaac gtttaccact gcaatccatt tcttccaacg ttttagctgt
     8461 attaaaaaat tttccattgc catacttttg cggcttggcg aaccttgttt caaaccatgc
     8521 actgttgtcc gtacggctgt ctgcatcacc aaacgtggca gtattactat ctaccaatac
     8581 ccaatccgcc gccaccgccg gcgttgccaa ccccaatgca aatactgcca agcacaacat
     8641 ctttttcatg tccgaccctc tcgtccttgt caacaaaata aagttaaatt gtaaaaaaaa
     8701 acgacttttt tcaagagaaa aatattccgt catgctatct gacaaccgtt tcagacggca
     8761 tcgtttccat ctgtacgccg ccgttataaa atagccttcc cgtcttttgg aacacgacca
     8821 tgaccgccct cccccgttac tccgttttcg gcaaccccgt tgcacacagt aaatcgccgc
     8881 aaatccatca gcaatttgcc cttcaggaag gcgttgacat tgaatacgaa cgcatttgcg
     8941 ccgacatcgg cggttttgca caggcggttt cgacattttt tgaaacaggc ggttgcgggg
     9001 caaacgttac cgtaccgttc aaacaggaag cgtttcatct ggcggacgag cattccgaac
     9061 gcgcattggc ggcaggtgcg gtcaatacgc tgattccgtt gaaaaacggc aagctgcgtg
     9121 gcgacaacac cgacggtttg ggtttggtcg gcgatatagt gaaggtgcaa cacacagaaa
     9181 ttgaaggcaa aaatatccta ttgctcggcg cgggcggcgc ggtgcgcggc gtgattccgg
     9241 ttttgaaaga acaccgtcct gcccgtatcg tcattgccaa ccgtacccgc gccaaagccg
     9301 aggaattgtc gcggcttttc ggcattgaag ccgtcccgat ggcggatttg aacggcggtt
     9361 ttgatatcat catcaacggc acgtccggcg gtctaaacgg tcagattccc gatattccgc
     9421 ccgatatttt tcaaaactgc gcgcttgcct acgatatggt gtacggctgc gcggcaaaac
     9481 cgtttttaga ttttgcacga caatcgggtg cgaaaaaaac tgccgacgga ctgggtatgc
     9541 tagtcggtca agcggcggct tcctacgccc tctggcgcgg atttacgccc gatatccgcc
     9601 ccgttatcga atacatgaaa gccatgtaat atgttccgca tcgtcaaatg gctgattgcc
     9661 ctgcccgtcg gcatctttat ctttttcaat gcctatgtgt acggcaacat cattacctac
     9721 cgcgccgtcg cgccccatcg gactgccttt atgtcgatgc ggatgaagca gtttgaacag
     9781 gaaggtcgcg atgtcgcact ggattaccgc tgggtgccct acaaccgcat ctcggtcaac
     9841 ctgaaaaaag ccctgattgc ttccgaagat gcccattttg ccgggcacgg cggcttcgat
     9901 tggggcggca ttcaaaacgc catcaggcgc aaccggaaca gcggcaaagt gaaggcgggc
     9961 ggctcgacca tcagccagca gcttgccaaa aacctgtttt taaacgaaag ccgcagctat
    10021 atccgcaaag gcgaagaagc ggcgattacc gcgatgatgg aagccgttac cgacaaagac
    10081 aggatttttg aactgtattt aaactcaatc gaatggcact acggcgtttt cggcgcggaa
    10141 gccgcgtccc ggtattttta tcaaataccc gccgccaagc tgaccaaaca gcaggcggcg
    10201 aaactgactg cccgcgtccc cgccccgctc tactacgccg accatccgaa aagcaaacgg
    10261 ctgcgcaaca aaaccaatat cgtgctcaga cgcatgggtt cggcagagtt gcctgaaagc
    10321 gacacggact gagtgccgcc gcaatataaa atgccgtctg aaatatcatt cagacggcat
    10381 atgctttctg cctttaccct cttctccaaa acgcaacggg gcggtcatta tcatttttgc
    10441 ccttctacat aacggtcaga cacaaaacgt gtccgacacg gacaacttcc caaaaacggc
    10501 agacaaaaaa ataaccgctt atcagcggtt ttttggtggc cagaggcgga atcgaaccgc
    10561 cgacacacgg attttcaatc cgttgctcta ccaactgagc tatctagcct ttatctgtct
    10621 cgttaagaga tgcgtattaa accaaatttc gggacaacac gcaagcggat ttgtcgatat
    10681 tttttatcca accgactaag tcgatgtttt aagtttaaat taatttcaca gaaaaatagt
    10741 gcaaatcggt tttttgccgt ttgttcggcg atattctgct tttcagacgg caaaacgctt
    10801 caatatttga agttctcgcc caaatagacg gcgcgcacct gttcgtttcc gaccaaatca
    10861 tcaggttttc ccgatgccaa caccgtgccg tctgaaataa tgtaggcccg atcgcagatg
    10921 ctgagggttt cgcgtacgtt gtggtcggta attaatacgc cgataccgcg cgacttgaga
    10981 aatccgatga ttttttggat gtcgatgacg gcgatgggat cgacgccggc aaaaggctcg
    11041 tccaacaaaa taaaatgcgg tttcatggca agtacgcggg cgatttcgac gcgtcggcgt
    11101 tcgccgcccg acaaagatgg ggcaggatta ttgcgcaaac gctcgatatt caggtcggca
    11161 agcagttttt caacttcgcc gtcgatgcgg cttttgtctt tggtactgat ttctaaaatg
    11221 gcgcggatgt tttgttcgac ggtcatttta cggaaaatag aggcttcctg cggtaggtag
    11281 ccgacaccca ggcgggcgcg ttcgtgtatg ggcaggtgac gcaattcctg cccgtccagc
    11341 atcacgctgc ccgcatcggc ggcaatgagt ccaacaatca tgtagaagct ggtggtttta
    11401 cccgcaccgt tgggcccgag caggccgacg acttccccgc tttcgatttc aagggagaag
    11461 cttttaacga cttggcgttt tttaaaactc ttttgcaggt tttgaacaac aaggcggctg
    11521 acgtttgcgc tcataaatat ctcttcaata tcttcggtcg gcatatgccg tcggaaccgg
    11581 gtttatgttt cagacggcat tttgggatta ttcggatttt tgcgtactcg aaggctggat
    11641 gatgacgctg accctgccgg atttggaagc ggattttgcg ccggattttg tactgccgct
    11701 gatggtatag acttcggttt tggtgttgta tgtaatcacc gcgccttcgg cgacatcgcc
    11761 gccgcgctgt actttggcat taccggttaa gactacggtg ctgcctgcag acgaataagc
    11821 aacgttgttt gcctgaccgc gcaccgtccc tttgcccccg tccaacgttt ggctgaagcg
    11881 tacgggcgag ccgtcggcct tcataaactg ttcgcctttg tcattacgcg ttacgttgac
    11941 gcgggcggcg gaaatattga gcgtaccctg tctgatgaca acgtttccgc taaatgtagt
    12001 gctttggttg gcttgattga gagaaccttg gtcggcctca atctgaatag gctgcctgct
    12061 gtcgccttga agggcaaaag cgggagatgc cgaaaaaaat gctattaaaa caaatagctt
    12121 acatatcttt tgtatcataa atcgtggctt tcactttaga tgagaagttc aacaggcctg
    12181 ttttgtggtc gtaagtcatg ccgcccgcct gcccgtgcga cgcaccgtat tgaaagctga
    12241 caggcgtatc ggtttgggca tattgagatt cggtatcgac gtgcagcttt tcggttttaa
    12301 ctttacccgc ctgccgtttg ccatcggcgg ttttggtcag cacaacgttg tttttaaaaa
    12361 gaacctgttt gttttcggta tggtaaacgg cttcatcgct gccgacttcg tacaacaacc
    12421 tgccttcttg gaagaacacg agatgcggcg aatcaaaatg gatgtcgctg ctttcgggaa
    12481 actgtttcgc gcctttcgcg ctcaaatgtt cttttaagta tccctgttcg tcaaaccgcc
    12541 tgccatccaa tccgtccatt gtgtattgcg gttcgtcggg attgagcctg acttcttcaa
    12601 tctcgacttc gctgatgcgt cccaaccatg ccgacaggct gcccaaggca accgccaata
    12661 tcaatgggaa cgcaattccg taccgccatc ttactttcat ttgatgtact cgttcaaagc
    12721 cgcgcccaaa gtcccttgcg cctgcataat caggtcgcac acttcgcgca ccgcacccgc
    12781 accgccggga cgtttggcaa tataggcggc gtgttgaagc gtaaaccaat gcgcgtcagg
    12841 gacggcaacc ggcaatccgc agcgcaccat caccggcaaa tcgaccacgt cgtcgccgac
    12901 aaaggcacac tcggcttctt ccacacctgc ctgcacgcgc agctcttcat acgcggcgcg
    12961 tttgtccgaa atgcctttga agtagtaatt tatgcccaac tgtttgacgc ggatgccgac
    13021 ggagggcgcg tcccgacctg tgataatcgc agtctgcacg ccgcttgcct gaagcatttt
    13081 cagaccgtgt ccgtccagtg tgtgaaacga tttgatttct tcgccgttat cgcggataaa
    13141 gatgcgcccg tcggtcaaaa caccgtccac atccaatatc agcagtttga ttttggcggc
    13201 gcgcgcctgt aattcgggag aaattgcctg catgggattt ccttgttttc agacggcaca
    13261 ttttagcaca aatgccgtct gaaacggctg caacgcctta tatagtggat taacaaaaat
    13321 caggacaagg cgacgaagcc gcagacagta cagatagtac ggcaaggcaa ggcgaggcaa
    13381 cgctgtactg gtttttgtta atccactata caatccgtgc cgccagcagg tcgtgcatat
    13441 tcagcgcgcc aatcagtacg ccgtcggcat cagtaaccag aagcccattc acatggtttg
    13501 cctgcatgac tttcagggct tcggtggcga ggcgttcggc ggagatggtt ttaggatgcg
    13561 tatgcatgac ttcgtctatc gaaagaccgg taaaattgtc gcattcttga aacaggcggc
    13621 gcaaatcgcc gtcggtgaat acgcctttca gacggccttg cccgtccgtt accgccagca
    13681 tccccagccc tttttcactc atgctgacga tggcttcttt caagggcgtg ccgagtcgga
    13741 cggcaggcag gccgccgcct ttgtgcataa tgtcggcaac gcgcaaaagt aggcgtttgc
    13801 cgaggctgcc ggcaggatgg ctcaaggcga aatcgtcggg cgtgaacgcg cgtgcgcgca
    13861 gcaggacgac cgccaacgca tcgcccaaag ccatgacggc ggtggtgctg gtggtcgggg
    13921 caagccccag cgggcaggct tcttgcgaaa ccgatgcggt gatatggatg tcggcatggc
    13981 gcgccatggt tgaatcgggg cgggcggtga tgcagacgag ggtaatgtct ttgcgtttga
    14041 gtgcggggat gatggcggcg atttcgtcgc tttcgccgga attggaaatc gcgacgacca
    14101 cgtcgttgtc cacaatcata cccaaatcgc cgtgtgccgc ttccgcaggg tggacgaaaa
    14161 acgcaggcgt gccggtcgag gccatagttg ccgccatttt gcgcccgata tgtcccgact
    14221 tgcccatgcc cgtgataacg accctgccct tgcagtgcaa caacgcgtcc gccgcaaggg
    14281 cgaagttttc gtccaaatcc gccgcaattt cgcgcaagcc ttccgcttcg gtgtgcaaca
    14341 cttcgcgcgc ccagtcgaga tattttccgt tttctgccat tgccatccgc ctttgctgta
    14401 acaaaactac ccaaaatttc cgaatcgggg tagcataagc attcaaaccc cgccatccat
    14461 ccaaaggaat cagagatgac cattttatcg gacgttaaag cattaggaca acaaatctgg
    14521 ctggacaacc tttcccgttc gctcgtgcaa agcggcgaat tggcgcaaat gttgaaacag
    14581 ggcgtgtgcg gcgtaacttc caatcccgcc atcttccaaa aagccttcgc cggcgacgcg
    14641 ctttacgccg acgaggtcgc cgccctcaag cagcaagacc tgaccccgaa acaacgctac
    14701 gaaacgatgg cggttgccga tgtgcgtgcc gcctgcggcg tttgccttgc cgaacacgaa
    14761 tccaccggcg gcaaaaccgg ctttgtcagc ctcgaagttt cgcccgagct tgccaaagac
    14821 gcgcaaggca cggttgaaga agtgcgccgc ctctacgccg ccatcgggtg caaaaacgcg
    14881 atgattaaag tgccggctac cgacgcaggc atcgatgcgc tcgaaaccat cgtttcagac
    14941 ggcatcagcg taaacctcac gctgctgttc tcacgcgccc aaaccctcaa agcctacgcc
    15001 gcctacgcgc gcggcatcgc caaacgcttg gcagccggac aaagcgttgc ccatatccac
    15061 gtcgtcgcca gcttcttcat ctcccgcgtg gacggtgcgc tggacacaac actgcccgat
    15121 tacctcaaag gcaaaatcgc catcgccctt gccaaagccg cctaccaaga ctgggcgcaa
    15181 tacttcggca gctcggaatt tgccgcgctg gaagcccaag gcgcaaaccg cgtgcagctt
    15241 ttatgggcat ctaccggcgt gaaaaaccca gcctatcccg acacgctcta cgttgacagc
    15301 ctgatcggcg cgcacaccgt caacaccgtt cccgatgcca cgctcaaagc ctttatcgac
    15361 cacggcacag ccaaagccac gctgaccgaa ggcgtagaag aagcacaagc gcagctcgcc
    15421 gaaaccgccg cgctcggcat cgatgtcgaa accttggcaa cgcgtttgca ggaagacggt
    15481 ttgaaacagt ttgaagaagc gtttgaaaaa ctgctcgcac cgttggttta aacttttcat
    15541 ccccgatgcc gtctgaaagg ctttcagacg gcatcgccct tttgaccgga cacagtgcgg
    15601 catatcaagg attcgcccta aaaatattaa tgggcaagaa gttaatgcac gacattaccc
    15661 gccaacacaa aaggaaatag aaccatagca aaagcactcg aaatcatttc acccaatgaa
    15721 atttattcag atctgatttt taaggatccg gtacctcccc atacaatata tacagaattg
    15781 atgaaactgg ttagtagaaa tgcaagtaat gagagaatcg cagatagagc cttcgatttt
    15841 ttctcgtccg gccttatgga tggttcagct accgaagagc aatataatgc cctatatgat
    15901 ttaaccttac ttgaagaacc cgggatggaa ctgaataaag atgagataat ggctttgatt
    15961 aatagtctta aataaagggg ccgtaccgga tatgtacaca ggacgtttcg cccccagccc
    16021 gaccgggctg ctccacatcg gctcgctgct gaccgccgtc gcttcctatg ccgatgcacg
    16081 cgcacacggc ggaaggtggc tggttcgcat ggaagacctc gatccgccgc gcgaaatgcc
    16141 gggggcggca agccatatcc tgcacacgct tgaggcattc ggattcgagt ggtacggaga
    16201 agtcgcctat cagagccgcc gttacgccct gtatgaagaa accctatgcc gtctgaaaac
    16261 cgccggactg gtctatccct gccattgcag ccgcaaagac tggcaggctg cggcagtcca
    16321 cggcgcagac ggattcgtct ataacggacg ttgccgcaat ccgcagcaac gccctgcact
    16381 gcaaggcaaa cagcctgcgt ggcgcatccg cgtccccgac cgcgacatcg gcttttcaga
    16441 cggcatcgtc ggcggttacg cccaaaacct cgcccgcgac atcggcgatt tcgtcctgct
    16501 tcgtgcagac ggttactggg cataccagct cgccgtcgtt gccgacgatg ccgaacaggg
    16561 cgttacccac atcgtccgcg gacaagacct gctcgtttcc actccgcgcc aaatctattt
    16621 gcagcagtgt ttgggcgttc cgacaccgca atatgcccac ctgccgctgc tgaccaacgc
    16681 acaagggcag aaatggtcga aacagacgct cgcccccgca ttggatttaa accgccgcga
    16741 acaactcctc cgccaagtgt tccgttacct caagctgccc gaagcaccgg aaaccgaccg
    16801 ccctgccgaa ctgctcgact gggcggtggc acactgggat atggacaaag tgccgaaaca
    16861 cgccattacc accccctaaa caccgcccga caaacgcaca aatgccgtct gaaactttca
    16921 gacggcattt cacatacagc cccgccctat tcgcgcgtat ttgcccgttc gacttcttcc
    16981 cacgcttcga gaatcaggcc gccgtcgttg tctttcaaga gcatcgcgtc ggaaagcata
    17041 cgccgccaag tccgcgcacc tttcaaaccg tgcatcagcc cgaggctgtg gcggacgatg
    17101 tggcgcaaga ttgtgccgcg tccggcttgg atctgggctt ggctgtatgt gtagaggcac
    17161 tgcaccaaat cggcgtattc gatcgggctg cgagtatcgc cgtaaaacaa tctgtcccat
    17221 tcgcgcatca ccatcgggtt gtggtacgcc tcgcgcccga ccattacgcc gtcaacgtgt
    17281 tgcaggtgtc cggcgattgc ttcgttggtg gtgatgccgc cgttgatgat gatttccagc
    17341 ccgggaaact cctgcttgag gcggtaaacg taatcgtatt tcaacggggg aacgtcgcgg
    17401 ttttctttgg gggaaagacc gtccagccaa gcgttgcggg cgtggacgat gaaggttttg
    17461 caggcggttt tgtcgcgcag cgtgccgacg aaatcggcaa cggtttggta ttcggtctgc
    17521 ctgtccacac cgatacggtg tttgacggta acgggaatac cgaccgcgtc ctgcatggcg
    17581 ttgaggcagt cggcaaccag cccgacttcg ttcatcagac acgcgccgaa cgagcctttc
    17641 tgcacgcgcg gactggggca gccgcagttg aggttggcct cgttgtaacc gtatgcctcg
    17701 gcggctttgg cggctttcgc caaatcggac ggatcgctgc cgcccagttg cagggcgacg
    17761 ggctgctcgc cttcgttgaa catcaaaaag cggtctttgt cgccgtaaac aatcgcaccg
    17821 gcattgacca tttcgctgta cagccaagta tttcgggtaa tctggcgggc aaggtaacgg
    17881 tagtgcctgt ccgtccagtc gagcatcggg gcgacggaaa ggcggcgcgg gggaagggtg
    17941 tgtgtattgt cgttcattgt cgatatttta tcattgcgga ttcaatttca atccggaaca
    18001 accttcattt ttggggaaat ccgcgtcatg aaagagtcca gcagtatggt taccggcccg
    18061 tcgttgcaga gcgacacctg catatgcgtg cggaaacgcc ctgtttcgac atgaatcccg
    18121 tgtccgcgca acagttccgc cgttcgcagg taaagctgct gcgcctgttc tgcaggtgcg
    18181 gcttgggaaa acgaaggccg ccgcccgctt gccgcatcgg cataaagtgt aaactgcgac
    18241 accagcagca ccgcgccgcc gacatctttc aaagacaggt tcaacttgcc cgcttcgtct
    18301 tcaaacacgc gcaaatgggc gattttgtcg gcgatatagt gcgcatcttt ttctgtgtcg
    18361 ctatgcgtta cgccgagcag cacgacgaat ccgccgtcga ttttgccaca ggtttccgtg
    18421 ccgttttcgg acacgacatc cacctttgca cctaccgttt tctgtatgac cgcacgcata
    18481 agccgtcctc cttacctgaa cgtgcaatgc cgtctgaaaa ggcttcagac ggcatcggta
    18541 ttggaaatgc cattacggtt tgacaccgtt ttcttccaaa atggcatcga ttttcaagcg
    18601 ggctttttcc tgtctcaaac cttgttccaa ctggtttctg accaactcga aaggctgcgc
    18661 gtcggggttt ttcccgacct cgctgagttt gaacagataa tagcgttcgc ccaatttgac
    18721 cggatcgcgg gtaacgtcgc cccgattcat cgctgcaaac tgcgaagcca gcggctcggg
    18781 aagctgctgc gccataatga aaccgtcaaa agcctgctcg tcgttcggat aacgcttcat
    18841 cagcccttca aaagacagcc ctttgagcag gagctgctgc gcctgacgcg cctcctcttc
    18901 ggttgcgaag ctgacctgct gcaatttgat catgcggatt tgccgctcat aaaattgacg
    18961 cagtgcgctt tcggaaaccg tttccgaacg ttccagaaaa cggacgtact cctcggcata
    19021 aaaagacgct tcggcgattt taaagcggtt ttggacatcc ttatccttat ccaaaccttc
    19081 cttcaatgcc ctgtttttca aaacttccaa agtttgcagc cgacggacgg catcgtttcg
    19141 gattgcctgc ccgtccggtt tttgggactg ctccgcatgc cggtctgcct gctgcatgat
    19201 ctgcgccacc agcgtatcca ccaaagccgg gtcgatttcg ggtgctttgg ctgccgcaaa
    19261 acctgccaac attgcagcaa taactgcggc agcggttttt ttctgtttca tggtattccc
    19321 tgtcctgcgc ggcggcaaaa cgcctgtctg aacgtcttgc cgccgtatcc cgttttcaga
    19381 attattttgc aggtttgatg tttgccttgc ccaacagtgc accgacggca cggtcaatcc
    19441 gttccgcctg aaggttgccg gcaatctgtc ctttcatttc atcaaaagaa ggcactttca
    19501 cctcgcggct gtcgttgaca taataaacgc cgtagaaatc gccgtttttc agcggcgttg
    19561 ccgtaaattc gccttttttc aagtccttaa ttgcctgata aagcggcgga acaccctgtt
    19621 ccaaatcttt cagcggcaca tatccgaccg gcgcgccggt ctgtttggta cggtcgttga
    19681 gggaatattg tttcaagacg gcatcgaaac cttttttcgc cttcaagtcg gcaaccgctt
    19741 tttttgcatt ttcttccttg tcggtcagga tttcgcccaa ctggacttcc tgcgtacctt
    19801 tgtaaaaacc gctgatattg tcatatgcgg cttttacttc ctgctcggaa accggttggg
    19861 ttttggcgat atgcagcgcg tatgcctcgc cgttcaagcc atattttacc gcctgccaaa
    19921 cggttttgaa ggacggtttc ttgtcgtcgc ccgacttttt cgcttcggca cgcaatttgg
    19981 caagcgcatt tttaaactct gccgaccggt cgagtttcag gcgtttcact tcctgtgcga
    20041 ccacggtgtt gaccacttcg ttttccagca gggattggcg cagttgcggc gtgtcttcgg
    20101 cacggctgtt ttccgcacgg aatgcggcaa cctgcgcatc gatgacggaa ctgtcgattt
    20161 tctgaccgtt gacggttgcc agcgtttggg caaacaggct gccggaacag gcaagcagtg
    20221 caacggaagt caggattttt gctttcataa tcttctctct ttaaatttta aattgaaatg
    20281 ttcgggcaga cttcattcag tccgccgtat ggaaatactc gtcaggggta gccgcccgga
    20341 tgccgagcgc gtgaatcatg ccgcctgaaa acaaatcttt gagcagcgat ttgaccgttt
    20401 tctggcggtt caggcggctt acgccttcaa aacggccgct gacgaccaaa acggcataat
    20461 gtccgccgcc ggtattgccc gcgtgtcctt tgtgcagatg gctctcatcg ccgatttcca
    20521 acaccagcgg atcgagcgtc tgcaggcgtt cgcggatcaa atcgacggcc ggcattattt
    20581 gaacaccgct ttaaacggct tgatcagcac ttcgctgtac acgcctgcat gaacataggg
    20641 atcgtcttcc gcccaagcct gcgccgcgtc caaagactcg aactgcgcca caatcaaact
    20701 gcccgaaacg cggttggagt cctccggcaa aggattcggg cctgccgtca acagccggcc
    20761 ttccgacttc agcgtctcca gccgtttgag gtgttcggga cgtgccgcca tacgcgcttc
    20821 atacacattc tccccgtctg ttgccagcaa cataaaatat tccacagtca atcctccttt
    20881 ttcagacagg tactcagata aataccctga ataataaaga aaacaagcat cagtgcagtc
    20941 gagccgaaca ttttatagtt gacccattgc gactcgaacc gggtaaacac aaaccagttg
    21001 gcgataccca taaaaatcag gaaaccgacc cacatatacg tcaatttcgc ccatacggca
    21061 tccggaagct gaatctccct gccgatactc gccttcaagc cgtttttacc ggcgaggtgg
    21121 ctgccccaca ggaacagcgc gcccagccaa aacaaaacgc tcggcttcca cataatgaag
    21181 cggctgtcgc ccaaaacaat ggttgcgccg ccgaatacga caatcagcac caatccgacc
    21241 cactgcatcg tatccagttt tttatatttc caatacagaa aagccgcctg aaccacaccg
    21301 gcaaccaagg caaccgccgt agcggcaatc atatttttgg taacggtata agtagcaaaa
    21361 aacaagatga cagacaaaag gtcgctgacg aatttcatat ccaaatgaga aagcaaatca
    21421 aataaacgct aatcatacag gcagaaccct gtttttgcac ctgcaaaacc aacaaatttt
    21481 gttttgattg atgcaaatat gcacagatgt ttttgaaaaa agatggagat atgtcataat
    21541 ttgtaaaaac ggcgaatgat tgccgtttaa aatgtggcgg cggtcggtac attcacatat
    21601 taaatgcccg ccggccgact gccccgccga tgcccgatga tgacgaaatt tacagactgt
    21661 acgcggtcaa accgtattca gccgccaacc cacaggggat acatcttgaa aaacaacaga
    21721 caaatcaaac tgattgccgc ctccgtcgca gttgccgcat cctttcaggc acatgctgga
    21781 ctgggcggac tgaatatcca gtccaacctt gacgaaccct tttccggcag cattaccgta
    21841 accggcgaag aagccaaagc cctgctaggc ggcggcagcg ttaccgtttc cgaaaaaggc
    21901 ctgaccgcca aagtccacaa gttgggcgac aaagccgtca ttgccgtttc ttccgcacag
    21961 gcagtccgcg atcccgtcct ggtgttccgc atcggcgcag gcgcgcaggt acgcgaatac
    22021 accgccatcc tcgatcctgt cggctactcg cccaaaacca aatctgcact ttcagacggc
    22081 aagacacacc gcaaaaccgc tccgacagca gagtcccaag aaaatcaaaa cgccaaagcc
    22141 ctccgcaaaa ccgataaaaa agacagcgcg aacgcagccg tcaaaccggc gtacaacggc
    22201 aaaacccata ccgtccgcaa aggcgaaacg gtcaaacaga ttgccgccgc catccgcccg
    22261 aaacacctga cgctcgaaca ggttgccgat gcgctgctga aggcaaaccc aaatgtttcc
    22321 gcacacggca gactgcgtgc gggcagcgtg cttcacattc cgaatctgaa caggatcaaa
    22381 gcggaacaac ccaaaccgca aacggcgaaa cccaaagccg aaaccgcatc catgccgtcc
    22441 gaaccgtcca aacaggcaac ggtagagaaa ccggttgaaa aacctgaagc aaaagttgcc
    22501 gcgcccgaag caaaagcgga aaaaccggcc gttcgacccg aacctgtacc cgctgcaaat
    22561 actgccgcat cggaaaccgc tgccgaatcc gccccccaag aagccgccgc ttctgccatc
    22621 gacacgccga ccgacgaaac cggtaacgcc gtttccgaac ctgtcgaaca ggtttctgcc
    22681 gaagaagaaa ccgaaagcgg actgttcggc ggttcgtaca ccttgctgct tgccggcgga
    22741 ggcgcggcat tgatcgccct gctgctgctt ttgcgccttg cccaatccaa acgcgcgcgc
    22801 cgtaccgaag aatccgtccc tgaggaagag cctgaccttg acgacgcggc agacgacggc
    22861 atagaaatca cctttgccga agtcgaaact ccggcaacgc ccgaacccgc tccgaaaaac
    22921 gatgtaaacg acacacttgc cttagatggg gaatctgaag aagagttatc ggcaaaacaa
    22981 acgttcgatg tcgaaaccga tacgccttcc aaccgcatcg acttggattt cgacagcctg
    23041 gcagccgcgc aaaacggcat tttatccggc gcacttacgc aggatgaaga aacccaaaaa
    23101 cgcgcggatg ccgattggaa cgccatcgaa tccacagaca gcgtgtacga gcccgagacc
    23161 ttcaacccgt acaaccctgt cgaaatcgtc atcgacacgc ccgaaccgga atctgtcgcc
    23221 caaactgccg aaaacaaacc ggaaaccgtc gataccgatt tctccgacaa cctgccctca
    23281 aacaaccata tcggcacaga agaaacagct tccgcaaaac ctgcctcacc ctccggactg
    23341 gcaggcttcc tgaaggcttc ctcgcccgaa accatcttgg aaaaaacagt tgccgaagtc
    23401 caaacaccgg aagagttgca cgatttcctg aaagtgtacg aaaccgatgc cgtcgcggaa
    23461 actgcgcctg aaacgcccga tttcaacgcc gccgcagacg atttgtccgc attgcttcaa
    23521 cctgccgaag caccgtccgt tgaggaaaat ataacggaaa ccgttgccga aacacccgac
    23581 ttcaacgcca ccgcagacga tttgtccgca ttacttcaac cttctgaagt acctgccgtt
    23641 gaggaaaatg cagcggaaat cgttgccgat gatttgtccg cactgttgca acctgctgaa
    23701 gcaccggccg ttgaggaaaa tgtaacgaaa accgttgccg aaatacctga tttcaacgcc
    23761 accgcagacg atttgtccgc attacttcaa ccttctgaag tacctgccgt tgaggaaaat
    23821 gcagcggaaa ccgttgccga tgatttgtcc gcactgttgc aacctgctga agcaccggcc
    23881 gttgaggaaa atgcagcgga aatcactttg gaaacgcctg attccaacac ctctgaagca
    23941 gacgctttgc ccgacttcct gaaagacggc gaggaggaaa cggtagattg gagcatctac
    24001 ctctcggaag aaaatatccc aaataatgca gataccagtt tcccttcgga atctgtaggt
    24061 tctgacgcgc cttccgaagc gaaatacgac cttgccgaaa tgtatctcga aatcggcgac
    24121 cgcgatgccg ctgccgagac agtgcagaaa ttgctggaag aagcggaagg cgacgtactc
    24181 aaacgtgccc aagcattggc gcaggaattg ggtatttgat tcccaactgc cctttcgcag
    24241 atcaaggatg ccgtttcaga cggcatcttt tttgccttat cggtgtaacg gataaagttt
    24301 gaaccggcac aggctcaaac agcaggccga cggcaacaaa atgccgtctg aaacccctaa
    24361 aggcttcaga cggcattggc ggcggcggta tcaggtattc gcatagacaa ccgaatcgtc
    24421 accgcttttc ttttgaatga actcgatttt gtatccgtcg gggtcttcga cgaaggctat
    24481 cacggttgtg ccgtgtttca tcgggccggc ttcgcggacg acgtttccgc cctgccgctt
    24541 cacacgttcg caggcttcgt aggcatcgtc cacttcaatc gcgatgtgtc cgtaagcgtt
    24601 gcccaagtcg tatcgttccg tatcccagtt gtgcgtcagt tccaaaaccg tgctgtcggt
    24661 ttcatcgccg taaccgacga aggcaagggt aaatctgcct tcgggatagt cttttcggcg
    24721 gagcagtttc atgcccaaaa cgttttggta gaaatcaagg gatttttcga gattgcccac
    24781 gcggagcata gtatggagta agcgcatttt tcgtgttcct cattggtggt ggttaacttc
    24841 gatttagtgg tggttaaact tcaattttat tcggggtaaa cgtctgccat ttgttgcagg
    24901 cgggacagtg ccagaaaaag acttgggatt tgaagtggca gttgcggcaa cggtacatca
    24961 cgctgcgctg tagctgccgt ccgataaccg aacgcatcat gtcggcatcg gctttccaag
    25021 ccggatccat atcgctgatt cttaaaccga gcaggcggta aacaccgttc aagtccggtt
    25081 tgcgccgaac aagctcgata gcagtttgtg cagcctcttt ctcctccctg agcaataagg
    25141 atttctcgta cacgacgtta atcaaatcaa gttccgggaa ggtctgcata taccctatca
    25201 ggcgatttaa tccttcttca agctttcctt gcgccgcata agcgtcataa agtttctctc
    25261 cgaccatgct caaataggca tgattttgct gctcgatggc ggcatacgct tcaaccgatg
    25321 caggaaaatt gccctgcctg tattcaatgt cgcctaaaat catattggca cgggtacatc
    25381 ttttgttggc ctcaagggct ttaccgacat taaaacgtgc aacatcaaaa ttggacttaa
    25441 acaatgaagc ctgtgcaagt tcgcaataaa actgtgcgat ttcaaactga taggtctgat
    25501 cgttatggct gagcagccgg gcggtttcaa ccgctttttc ccaatccctg tcctgttggt
    25561 agatattgag caggtgctgt ctggcttcac gcgccatttc accgtcttgc agccccaaaa
    25621 aaatctgttc ggcacgatcg accaaacccg cgctttggta gttttgcgcc aattcaaaca
    25681 ggacgcgcgc gcgcttttcg ccgaccgtat cgggagaatc gagcattgtc cggtgtatgt
    25741 tgatggcttt gtcgttttcg ccgcgctggc ggtaaagttt gccgagggtg aggttcaaat
    25801 catacgattg cggccggccg tcgacgactt ccgccaactc ccttgccgcg cgcccgctgt
    25861 tgcggtcgac caaagcgtcc aagcttttat aaaatcccga agggatgctt tttgcctgct
    25921 tcaatacggt tttcatatcc acgcgggcgg caaaccagcc catcgcaaag aagacgggca
    25981 aaaggataat cggcagcagg ataatccaca attcgttgtc catatcggct ttcttaaggc
    26041 tgtttggcgg gttcggcagc attttgtgcc ggcggtgcag tcaattcctg tccgctcaag
    26101 cgcgcacttt tcttcacttc cgcacgcagg cggctgtttt cgccgcgcaa ggacagcaac
    26161 cgcccgaaca gggcaaacat tccaaaaata ataccgacta caaatgcgcc gaacaatacg
    26221 acaatcaacg gcaaatcgaa tttttgcccc ggcaggtagg aaaaggtaac ggcatccgta
    26281 ttaatgacgg caagcagcag gaagagcagc aggataatga ttttgatgac ggtatagata
    26341 agtttcatgt ttgctccgta aataaaaacg ccgcccggac agcttcgcac tctgaaccaa
    26401 gattgcgcta gtttaaacga tttgcacggt ttcggatagg atgcggcagc gtgttcggac
    26461 gacatacgga gtatgcgatt gccgacaatt ttaccaaata caccgttcct ttccatttga
    26521 aaaataacgg attggacacc gcatcgacag aaaaacccgc cgcgcacttg tcaaaaccct
    26581 gtttgcaggc gtatctttac aatcttcaaa ttcaaaccgt tcattgaaac atatcagaat
    26641 aagaaaggct ttacatcatg agcagacccg tacccgccgt attcggcagc gtttttcaca
    26701 gtcaaatgcc cgtcctcgcc taccgcgaag gcaaatggca gccgaccgaa tggcaatctt
    26761 cccaagacct ctccctcgca ccgggcgcgc acgccctgca ctacggcagc gaatgtttcg
    26821 agggactgaa agccttccgt caggcagacg gtaaaatcgt actgttccgt ccgactgcca
    26881 atatcgcgcg tatgcggcaa agtgcggaca ttttgcacct gccccgcccc gaaacccaag
    26941 cctatctcga tgctttggtc gaactggtca aacgtgccgc cgacgaaatt cccgatgcgc
    27001 ctgccgccct gtacctgcgc ccgaccttaa tcggtaccga tcccgttatc ggcaaggccg
    27061 gctctccttc cgaaaccgcc ctgctgtata ttttggcttc ccccgtcggc gactatttca
    27121 aagtcggctc gcccgtcaaa attttagtgg aaaccgaaca catccgctgc gccccgcata
    27181 tgggccgcgt caaatgcggc ggcaactacg cttccgccat gcactgggtg ctgaaggcga
    27241 aagccgaata cggcgcaaac caagtcctct tctgcccgaa cggcgacgtg caggaaaccg
    27301 gcgcgtccaa ctttatcctg attaacggcg atgaaatcat taccaaaccg ctgaccgacg
    27361 aatttttgca cggcgtaacc cgcgattccg tactgacggt tgccaaagat ttgggctata
    27421 ccgtcagcga acgcaatttc acggttgacg aactcaaagc tgcggtggaa aacggtgcgg
    27481 aggcgattct gaccggtacg gcagccgtca tctcgcccgt tacttccttc gtcatcggcg
    27541 gcaaagaaat cgaagtgaaa agccaagaac gcggctatgc catccgtaag gcgattaccg
    27601 acatccagta tggtttggcg gaagacaaat acggctggct ggttgaagtg tgctgatgct
    27661 ttaaataaaa aatgccgtct gaaacccgtt tggcgtttca gacggcattt tcgcatccga
    27721 accgtttccg ctgcacctgc agcaagtcgg cacaaaggca atcggttaaa acaagcgtcc
    27781 gcatttccca tcccgcctgc cgtaagtcgg gcatttccct agaaatacgc ttcagacggc
    27841 aaaacgccgc ccgaaaccga tatgcggcac ggacggcgca cggatttgaa aacggcggat
    27901 tatccctcgg tgctcaaggc attgatgctg taaccgccgt caacgtaagt gatttcgccg
    27961 gtaatgccgg acgacaggtc ggacagcagg aaggcggcgg tattgccgac ttcttcaatg
    28021 gtaacgttgc ggcgcagcgg attgtgggcg gcgacgtgtc ccaaaagttt gccgaaatcg
    28081 gcgatgccgg aggcggcgag tgttttgatc ggaccggcgg aaatgccgtt gcagcggatg
    28141 ccttctttgc ccagacaggc ggcggtaaaa cggatgcccg cctcaaggct ggcttttgcc
    28201 atacccatca cgttgtaatt cggaatcgcg cgcaccgcgc ccaagtagct cagggcaacg
    28261 atggcggagt ttctgccgcg catcatcgga cgggcggctt ttgccaatgc gggcaggctg
    28321 tatgcggaaa tttcgtgtgc ggtgttgaac gcttcgcggc tgatgctatc gaggaagtcg
    28381 ccgctcaagg cttctttcgg cgcaaaaccg atggaatgca ccaaaccgtc caagccgtcc
    28441 caatgtttgc ccaaatcggc gaacacttgg ttgatttcgt cgtcgctggc gacatcgcag
    28501 cggaatacga gttcggaatc caattccgcc gccattttgc ggacgcgctc ttccagtttg
    28561 tccacaacgt aggtaaacgc caaatccgcg ccttgttcgc ggcaggcttt ggcgatgccg
    28621 taagcgatgg aacgctcgga aatcatgccg gtaatcagaa tttttttgcc ttgcagaaag
    28681 cccattttgt atccttcaaa tagtgttcgg ttcgttctaa accgttgatt atagcaaatt
    28741 gtccctgttt ctgtgttttc acgttgcagc gtgcaaacgg caatgccgtc cgaagcggat
    28801 ttcagacggc atcggacgtt tcaaatacgg tttaaggcat cagatgccgc gcaacaattc
    28861 gttcacactg gttttggcgc gtgtttgcgc gtccacacgt ttgacgatga cggcgcagta
    28921 aaggctgtgg ctgccgtctt tggaaggcat actgccggat acgacaaccg aacctgccgg
    28981 tacgcggcct tggtagattt cgcctgtggt gcggtcgaag attttggtgg attgaccgat
    29041 gaacacgccc atcgaaatca cgctgccttc ttcgacaatc acaccctcaa cgatttcaga
    29101 acgcgcaccg atgaagcagt tgtcttcaat gatggtgggg gcggcctgca ggggttcgag
    29161 tacgccgccg ataccgacgc cgccactcaa gtgcacgttt ttgccgattt gcgcgcaaga
    29221 gccgacagtc gcccaagtat caaccatcgc gccttcgtcg acgtatgcgc cgatgttgac
    29281 gtaagacggc atcagcacga catttttcgc cacaaagctg ccgcgtcggg caaccgcacc
    29341 cggaacggcg cggaagcctg cgtttttgaa ctcgtcttca gaccagtcgg caaacttggt
    29401 cggcactttg tcgaagtatt tgttcacgcc gtcgttgagg acttcgttgt cttggatgcg
    29461 gaaggacagc aacacggctt ttttcgccca ttcgttgact ttccactcac ccacgcccaa
    29521 gcgttcggca acgcgcagtt tgcctgaatc gagttggcgg atggtttcca acacggcttc
    29581 tttgacttcg ggagtaacgg tggtcggggt aatgtccgcg cggttttcaa aggcggtttc
    29641 gataatgttt tgcaaagaca taatgtttcc ttatgtgaga tgtttcgtgg gacgcttgcc
    29701 ggtattttca agcagtatgt tcatttgaaa tgaatgcgct attgtgccac gcaaacgcat
    29761 attcgtcagc aatacacggg cggttgccga acattggcgt ttcggaatcg ggcgggcggc
    29821 aggtattgcc ttttccgtgc ccgaatcaat acagaacggc ggcggcactt ttatgccgcg
    29881 ccgccgcctt tcagacggca ttcgcggtaa aacgccatca gcccttcggt cgaggcatcg
    29941 tgtacggacg tgccgccttc cagttcgccg atgatggttt tcgccaactg tttgccgtac
    30001 tcaacccccc actgatcgaa ggggttgacg ttccatatcg cgccttggac gaaggttttg
    30061 tgttcgtaag ccgccatcag catacccaaa ttgtagggcg tgaggcggtc gagcaggatg
    30121 ctgttgctgg ggcggttgcc agggaactct ttgtgcggcg caaggcgttc gcgttccgct
    30181 tcgggcaaat ctgccagttc ggcgcgtgct tcgtccaagg ttttgccctt catcagggct
    30241 tccgcttggg caaaggcgtt ggcaacggta aaacggctgc gtccgtcctc cacgccctgc
    30301 gccgtcaccg ggacgataaa gtcgcaggga attaggcgcg tgccttggtg gagcagttgg
    30361 aaataggcgt gctggcagtt gaccccttca ccgccgaaca cgatgccgcc cgttttgcac
    30421 acggcgggac tgccgtctga agcgcggctt ttgcccaaac tctccatatc gagctggttc
    30481 agccacaccg gcagcaggcg caggttgtgg ctgtacggaa cggcggtctg cccgtccgcg
    30541 tgttggaaat tgttatacca cacggcaatc agtgccatca gaacgggaat gttgcggcgc
    30601 ggcggcgtgt ggaaaaaatg gctgtccatc gcgtgcgccc ccgccaacaa ctcgcggaaa
    30661 cgcgccccgc cgaccgcaac catcacgggc aaaccgacgg gcgaccagac ggaatagcgt
    30721 ccgcccaccc agtcgtacat cgcaaacacg cgttccgccg cgataccaaa agccgcagct
    30781 gccgcagtgt cggcagacac cgcgcaaaaa tggcacgccg tttcggattc cgagaacccc
    30841 gcaccgcgat accacgcctt gactgcctgt gcattgagca gggtttccgg tgttttgaag
    30901 gacttgctgg caacgcaaaa cactgtcgtt tcggggttca gacggcataa aaccgcatcc
    30961 aggcaggcag gatcggcgtt ggcggcaaaa tggacggtga tatgccgtct gaacggctca
    31021 agtgcctgca cgcacattgc cggcccgagg tcggatccgc ctatgccgat gtggacaaaa
    31081 tccgtaatcc gttttccggt tgtcccctga tacgaaccgt cgtccaaact gtgtgcaaac
    31141 ttcaacgcac gatttaactc gcggcggatt tcgggcaaca cgtccctgcc gtccgcataa
    31201 acggcacccg caccgtcggg caggcgcaaa gccgtatgca gcgcggcacg cccctcactg
    31261 ttgttgacct tcgcacccgt ccgcaaagca cgcattttcc cttccaaatc cgccgtctcc
    31321 gcaagacggc agagcagttg cagcgtatct tcgcccaaac ggtttttgct gtaatcgaac
    31381 aacatcccgt ccaaacgctc atgcatacgc tcaaaacggt cgggttcgca ggcaaagcgg
    31441 tcgcgcaaaa ggacatgacg cgtatcctga taatggcgtt cgagcgcata ccatgcacgg
    31501 gtaaaagcat tcatctgttt tccttgattt ttcagaaccg gattaaaatg tagtagaatg
    31561 tagtttaaca aacggcagcg gctttggcga atctccggaa caccgcccgc cgcgtccaat
    31621 agcaccttat ttccatatgg cgaaaatata gtgaattaac aaaaatcagg acaaggcggc
    31681 gagccgcaga cagtacaaat agtatggaac cgattcactt ggtgcttcag caccttagag
    31741 aatcgttctc tcttgagcta aggcgagcca acgccgtacc ggtttttgtt aattcactat
    31801 accctccggc cggcatttga cccgcaacaa tatcctgcaa gatttattgt gtacgcataa
    31861 atgccggaca gccgcctaaa taaggtataa tcgcatccga ttctgtcccg tttgccgccc
    31921 gcaggcggga tgtcggcggt tttcatctta ccgggaacac gccggtactg ttttttaacg
    31981 atgtttctta cattttattc caattacttt acggggctag aatatggcta aaaacggagg
    32041 attttctttg ttcgcaaaga aagaaaaacg ctttatcttt gaaggcaggc attccgcctc
    32101 cgacaaactg gtcaacggcg aagtatccgc gtttaccgaa gaagaggcgc gcaaaaaact
    32161 ggcaaaacgc ggcatccgcc cgttgcagat tacccgtgtg aaaacaagct ccaagcgcaa
    32221 aatcacacaa gaagacatca ccgttttcac ccgccagctg tccacgatga ttaaagcggg
    32281 cctgccgctg atgcaggcat ttgaaatcgt ggcgcgcgga cacggcaacc cgtctatgac
    32341 ggaaatgctg atggaaatcc gaggcgaagt ggaacagggc agctcgttga gccgcgcatt
    32401 ctcaaaccac ccgaaatatt tcgaccgctt ctactgcaac ctggttgcgg cgggcgaaac
    32461 gggcggcgta ttggaaagcc tgctggacaa attggcaatt tacaaagaaa aaacccaggc
    32521 catccgcaaa aaggtaaaaa ccgcgctgac ctatccggta tccgtcatcg ccgtcgccat
    32581 cggtttggta ttcgtgatga tgattttcgt actgcccgcc tttaaagaag tttacgccaa
    32641 tatgggcgcg gagcttcccc cactgaccca aacagtgatg gatatgtccg actttttcgt
    32701 ctcatacggc tggatggtgc tgatcgcact gggctttgcc atatacggct tccttaaatt
    32761 gaaggcgcgt tcgattaaaa tccaacggcg tatggatgcc atactgctgc gtatgccgat
    32821 tttcggagac attgtccgca aagggacgat tgcccgctgg ggcaggacga cggcgacgct
    32881 gattgcggca ggcgtgcctt tggtcgatgt attggactcc actgccggcg cggcgggcaa
    32941 tttaatctat gaagaagcca cccgggaaat ccgtacgcgc gtcatccaag gtttgtctat
    33001 gacttcgggg atgcgtgcga cggaactgtt ccccaatatg atgttgcaga tgtcttccat
    33061 cggcgaggaa tcgggttctt tggacgatat gctcaacaaa gccgccgaat tttacgaaga
    33121 cgaggtggac aatgcggtcg gcaggctgtc cgctatgatg gagccgatca ttatcgtgat
    33181 tttaggcttg gtcatcggca cgcttctggt cgccatgtat ctgccgctgt ttaatttggg
    33241 caacgtggtc gcctgatttg ccgcacagat ccggcgcgga ttcgctctgc gccggtttgt
    33301 ttttgttttg aatacattga gaacaaaata tgtctgattt gtctgtattg tcgccgtttg
    33361 ccgtgccttt ggcagcggtg ttcgggctgc tggtcggcag tttcttaaac gtcgtcattt
    33421 accgcgtgcc ggtcatgatg gaacgcggct ggacggtatt tgccaaagaa tatttaaacc
    33481 tgccgctgac cgaagaggaa agccgtacct tcaacctgat gaaaccggat tcctgctgtc
    33541 ccaaatgtcg cgtgccgata cgcgcgcggc agaacatccc gattgtcagc tacctgctcc
    33601 tgcgcggcaa atgcgcttcc tgccaaacca aaatcagcat acgttatccc ttaatcgagc
    33661 tgctgaccgg cgtattgttc gggctggttg cctggcaata cggctggtct tggattacat
    33721 tgggcggttt gatactgacc gcgtttctga tttccctgac ctttatcgat gcggacaccc
    33781 aatacctgcc cgactcgatg accttaccct tgatttggct ggggctgata tttaatttgg
    33841 acggcggctt cgtgcctttg cagtctgccg ttttaggtgc ggttgccggc tatggttcga
    33901 tatggctttt atgcgcggta tataaactga ttaaaggcga aatcggtatg ggcaacggag
    33961 atttcaaact gattgccgca ttgggcgcgt ggctcggcat atccgcattg cccgtgctga
    34021 tttttgtttc ctcgctgatc ggtttggtcg cggcaatcgt tatgcgcgtc gccaaggggc
    34081 agcattttgc cttcggcccc gcactgacag tttcgggctg gataattttt acggcaaacg
    34141 attccgtatg gcgggcggtc aactggtggc tgacccatcc ggtgctgtaa gatgacggta
    34201 tgggtcggac tgaccggcgg aatcggcagc ggcaaatcgg cagccgcgca atgttttgcc
    34261 gatttgggcg tgccgcgcat cgatgcagac gcggcggcgc actcgctgac ggcttcagac
    34321 ggcatcgccc tgccggaaat caggcggctg ttcggcgaca ccgttttcga cacacagggt
    34381 ttgttgcggc gcgacatatt gcgtaaagaa gtctttgcct ccccatcgcg aaaagccttg
    34441 ctcgaatccg tgatgttgcc gctgattttc tcagaaatca aaaaacagca agaaaccttt
    34501 accgatgcgg cttacggcat tgtcgaaatt ccgctgctga cggaaaagcg tcaatttatc
    34561 atcctgatac ggcgtgtcct gaccataagt gcccctgtgg aaaaacgtat cggcagggtg
    34621 atggcccgca gcgggctgac gcgcggcgag gtggctgccg tcatcagcca tcaggcatcc
    34681 gaatccgaac gcctgctgct tgcagacgat gtgctgctca atgacggcag cctcaaaagc
    34741 ctgcgtgaga aaacaatgcg cctgcacgcg ttttattcag ggattttcgc ctcaaaacca
    34801 acacaaggaa aacacaatgg ctgaatcgcg gcaaacacgc cttcaagtca aatgcccgac
    34861 ctgtcaaacg gcagtagtat ggaaaccgga aaacgcattc cgccccttct gttcgcaacg
    34921 ctgcaaactg atcgacttgg gcggatgggc agacgggaaa tatacggttt ccggccaaac
    34981 ggaaagtttg ccggaaatat ccgaacccga catggcatac cgctgaccgc cccgccttcc
    35041 cggcaaacac cctgaaagtc aaatgccgtc tgaaacaaac acgcttcaga cggcattttc
    35101 attctcaaac ctaatcgttg gtatttgccg ttacctcttc caatgaagta atgccctgca
    35161 taactttcaa aataccggcc cggcgcaaat ccaccatacc ctccttatag gcaacgtcca
    35221 aaatatccac ttccgtaccg ttgttcataa tcacacgctg catttcttcg ctgatgggca
    35281 taacctcata cacgcccgca cgccccttat aaccctgccc ccggcaacgg tcgcaaccga
    35341 cggcgcggta aagtttccaa tcttttgcaa ggtcctcatc ggtgaagccg acttccttca
    35401 aagcagaggc agacggacgt tccacttcct gtttgcagct cgaacacagc ctgcgtaaaa
    35461 gacgctgcgc cataatcagg ctgaccgaac tggcaatatt aaacggcgcg acacccatat
    35521 tcagcatacg cgacaacgtc gccggcgcat tattcgtgtg cagtgtggaa aacaccatat
    35581 gccctgtttg tgccgcctta atcgcaatat cggcagtttc caaatcacga atctcaccga
    35641 ccataatgat gtccgggtcc tgacgcagga aagacttcaa agcagcggca aaagtcagac
    35701 cctgcttatc attgacgtta acctgattga tgcccggcag gttaatctcg gcagggtctt
    35761 ccgccgttgc aatatttacc gactccgtat tcaaaatatt caaacaggta tagagcgaca
    35821 ccgtcttacc cgaacccgtc gggccggtta ccagcaccat cccgtaagga cggtgaatcg
    35881 cctccaacaa caattttttc tgaaacggct caaaaccgag ctggtcgatg ttcaaagacg
    35941 cggcatcgga attcaaaatc cgcatcacaa ccttttcgcc aaacagcgtc ggcaatgtgc
    36001 tgacacggaa atcgacaggc ttgccgccct tttgaaaggt cagctgcatc ctgccgtcct
    36061 gcggtatccg tttttcggaa atgtccaaac gcgacattac cttaatccgg gaagcaagct
    36121 gcccccttac cgcaatgggc ggctgaacca cctcgcggag ctgcccgtcc acacggaaac
    36181 ggatacgggc attgtgttcg taaaactcga aatggatgtc cgatgccccg ctgcgcaagg
    36241 catccgacaa agtcttatgg ataaacctcg gaacagggcc gtcttctgcc tcctcgttgt
    36301 cgatatacag ggtgtggctt tcctcttcct cctgcccctc cccaagctcc tgaagcagcg
    36361 atgtcgaacg cgaacccacc caatcgagca aacccgccaa ctggtcatcc tcgacaatga
    36421 ccaactcaac ctcaatccct gcggcagaaa cggttttctg aatttgcggc atctgtgtcg
    36481 gatcggaaac cgcaaaaaat actttgtcgc cccgacggaa aaccggcaca cagtggaact
    36541 ccaccatctg ctcctccgtc aacaccccca tcagcaccct gtggcgcgga taatgacgca
    36601 aatcaagaat cgaataactg aacaccctcg caatcaatgc cgcaagcgac ttgggcgaaa
    36661 tgacaccgtc tgaaaacagc atcggcaaca cttccttacc cgcctgcgac tcattgtagt
    36721 aatgctcggc ctgctcaaca gtaaccacct ggttttgaac cagaatcctc agcaaaccta
    36781 cgctcatacg accttatccc caaatttatt cattgttata cctgtacagc ttttatagtg
    36841 gattaaattt aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa
    36901 ggtgctgaag caccaagtga atcggttccg tactatctgt actgtctgcg gcttcgtcgc
    36961 cttgtcctga tttttgttaa tccactatat cataaaacaa cagatttcca agccggaaca
    37021 tcttttacga agcctgaaaa tcatttcatg attctaccgt cctaaaggtc ggtttttcaa
    37081 gcaggaagaa aaagttttca gatggcaaaa aagccctcca acactgaaag gccttatatc
    37141 ggaaacttcc cgcaacacgg gaaacagaca aatgcaaatc gtcaaacctc gccaacagga
    37201 atcgaacctg tattttacgc ttaggaggca tacgttctat ccgttgaact atggcgagcc
    37261 gaaatgaaaa ggagatttta accctttccg acgacaaaag acaagatgcc ggccgcaatc
    37321 agcggcccga cagggatacc gcccataaat gccaccccga taaccgtccc gattaacagc
    37381 cctgtaatta aaacaggctg ctgtcccatc aaaggcacgc cgcgtcccgc cagccaagcc
    37441 acgaaaatac cgataaaaac ggcggatatc attttaaaat tcaaaaattc ggcaacagga
    37501 ggaacctgcg cctttcctga aaccaacgga ctcaaaaccc ctatggtcaa aagaatgata
    37561 ccgagattca acccatgctt ctcgaccaac gggacaaact gtaccaatgc cgtctgctgc
    37621 atcagcagca atatggttgc cgagatggta atcgaattgt tgttgctgac cacccccaac
    37681 agaatcagcg taaccagaaa caggggaaca aaactgaaat tcatcgcctc aagacaccct
    37741 gaccaaagat tccttcgcca gctgaatcat ggcatccttc acttcgctgt cgggcagggc
    37801 atccaaggcg gtaacggcac aatcgactgc tttgcgcgcc tcgcctatcg aatatgccaa
    37861 agcatccgaa cggacgacat aatcgtggat tttctcaaaa tagctgcgat ctgcattttc
    37921 caaagcagta cgcacatcgt tcgcaacctg ttcggaaccc tgacgcatca gataaatcaa
    37981 aggcaaagtc ggttttcctt ccgccaaatc gtcgccgacg tttttgccgg tttcttcggt
    38041 ttcgccagaa tagtccagca catcgtcaat aatttggaat gccgtaccga catacatacc
    38101 gtagtctttc aacgcctgtt cgtgttcggg ggaagccttg cccaaaattg cgccgacttg
    38161 agcggcagct tcaaacaatt ttgccgtttt atattggatg acttggatat attgttcttc
    38221 ggtaatgtcc gtattgccga tgttcatcag ctgcatgact tcgccctcgg caataatgtt
    38281 ggttgcatcc gccatcactt ccaaaacgcg catactgccc gaggcaacca tcagttgaaa
    38341 ggcgcgcgtg tataaaaagt cgccaaccaa cacagccgcc gcattgccga acagattgtt
    38401 tgccgttgcc cgcccacggc gcaaatcgct ttcatcgacg acatcgtcgt gcaggagggt
    38461 ggaagtgtgg ataaactcga ccatcgccgc cagcgaatac agtttctcgt catcataacc
    38521 gaccgcctta cccgccaaaa tcgtcataat cggacgcagg cgtttgccgc ccgcgctgat
    38581 gatatatgta ccgatttgcg aaatcagtgc gacatcggat tgcaccgcac ggttgatgac
    38641 ttcattgact ttggcaaggt cttcaggcag atgtcgctgg aaatagggca gattctcgag
    38701 catggcagac tctcaggttt acgtttccgt caaacggcag ataccgcttg aaagctgatt
    38761 caaatagcgg gcaattatat cagcaaagcc atcagggtac atcttttcag acggcatatg
    38821 gttatccgaa ccgcgcaaac tttgacaaaa caggcaaata acaatacaat gacgagttcc
    38881 gcacaatggt gtgcggatat taaccataac ccttatggag ttgagtatgt acgcggtcgt
    38941 aaaaaccggc ggcaaacagt ataaagtttc cgtcggcgaa aaattgaaag tagaacagat
    39001 accagccgaa ctcgacagcc aaatcgaact gaccgaagtt ttgatgattg ctgacggcga
    39061 atctgtaaaa gttggcgcac cctttatcga aggtgcaaaa gtaacggcta aagtagtggc
    39121 acacggtcgt ggcgaaaaag tccgcatctt caaaatgcgc cgccgcaaac actaccaaaa
    39181 acgccaaggc caccgccaaa atttcaccca aatcgaaatc gtggcaatcg cctaacctta
    39241 aaacttagga gcattacaaa atggcaagta aaaaagcagg cggcagcacc cgcaacggtc
    39301 gcgattcaga agccaaacgc ttgggcgtta aagcctacgg caacgagctg attcccgcag
    39361 gttccatcat cgtacgccaa cgcggtacca aattccacgc aggcgacaac gtaggtatgg
    39421 gcaaagacca cactttgttc gccaaagttg acggttatgt cgaattcaaa accaaaggcg
    39481 cgctgaaccg taaaactgtc agcatccgtc cttacaccgg ttctgaagaa taatccaacc
    39541 gatacctgaa gccgcatctt ttcacgatgc ggct
//
LOCUS       DAWFJW010000005        38872 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFJW010000005 DAWFJW010000000
VERSION     DAWFJW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.005"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(124..897)
                     /locus_tag="WGR19_000188"
     CDS             complement(124..897)
                     /locus_tag="WGR19_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0797792.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(996..1619)
                     /gene="rsmG"
                     /locus_tag="WGR19_000189"
     CDS             complement(996..1619)
                     /gene="rsmG"
                     /locus_tag="WGR19_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0797793.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1715..2200)
                     /locus_tag="WGR19_000190"
     CDS             complement(1715..2200)
                     /locus_tag="WGR19_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797794.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDAFALAFGRAGLLVSV
                     SDGGFGWRGGWDGTVWFVYGVFAFLNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            2306..2381
                     /locus_tag="WGR19_000191"
     tRNA            2306..2381
                     /locus_tag="WGR19_000191"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2339..2341,aa:Thr,seq:cgt)
     gene            2455..3582
                     /locus_tag="WGR19_000192"
     CDS             2455..3582
                     /locus_tag="WGR19_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0797795.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            3763..4320
                     /gene="frr"
                     /locus_tag="WGR19_000193"
     CDS             3763..4320
                     /gene="frr"
                     /locus_tag="WGR19_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0797796.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPI
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4376..5122
                     /locus_tag="WGR19_000194"
     CDS             4376..5122
                     /locus_tag="WGR19_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0797797.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5126..5923
                     /locus_tag="WGR19_000195"
     CDS             5126..5923
                     /locus_tag="WGR19_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0797798.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAAGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5928..7163
                     /locus_tag="WGR19_000196"
     CDS             5928..7163
                     /locus_tag="WGR19_000196"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0797799.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSLHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSSTMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDTLSALTFQKPDFGRFPCLKL
                     AYEAMNAGGVAPCVLNAANETAVAAFLDGRIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7198..8538
                     /gene="rseP"
                     /locus_tag="WGR19_000197"
     CDS             7198..8538
                     /gene="rseP"
                     /locus_tag="WGR19_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0797800.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYAVAKLCGVKVLRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8595..10988
                     /gene="bamA"
                     /locus_tag="WGR19_000198"
     CDS             8595..10988
                     /gene="bamA"
                     /locus_tag="WGR19_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0797801.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTQGSAIIKNLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKQYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11054..11554
                     /locus_tag="WGR19_000199"
     CDS             11054..11554
                     /locus_tag="WGR19_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0797802.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11587..12633
                     /gene="lpxD"
                     /locus_tag="WGR19_000200"
     CDS             11587..12633
                     /gene="lpxD"
                     /locus_tag="WGR19_000200"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0797803.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTTVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDTGQDSK"
     gene            12667..13116
                     /gene="fabZ"
                     /locus_tag="WGR19_000201"
     CDS             12667..13116
                     /gene="fabZ"
                     /locus_tag="WGR19_000201"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0797804.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            13203..13979
                     /gene="lpxA"
                     /locus_tag="WGR19_000202"
     CDS             13203..13979
                     /gene="lpxA"
                     /locus_tag="WGR19_000202"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0797805.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFTQSTRGIIR"
     gene            14337..15728
                     /locus_tag="WGR19_000203"
     CDS             14337..15728
                     /locus_tag="WGR19_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0797806.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            15807..17063
                     /locus_tag="WGR19_000204"
     CDS             15807..17063
                     /locus_tag="WGR19_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0797807.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            17244..18053
                     /gene="zupT"
                     /locus_tag="WGR19_000205"
     CDS             17244..18053
                     /gene="zupT"
                     /locus_tag="WGR19_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0797808.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            18150..20987
                     /locus_tag="WGR19_000206"
     CDS             18150..20987
                     /locus_tag="WGR19_000206"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0797809.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            21129..21320
                     /locus_tag="WGR19_000207"
     CDS             21129..21320
                     /locus_tag="WGR19_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797810.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(21317..22237)
                     /gene="oxyR"
                     /locus_tag="WGR19_000208"
     CDS             complement(21317..22237)
                     /gene="oxyR"
                     /locus_tag="WGR19_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0797811.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(22241..22504)
                     /gene="minE"
                     /locus_tag="WGR19_000209"
     CDS             complement(22241..22504)
                     /gene="minE"
                     /locus_tag="WGR19_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0797812.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            complement(22508..23323)
                     /gene="minD"
                     /locus_tag="WGR19_000210"
     CDS             complement(22508..23323)
                     /gene="minD"
                     /locus_tag="WGR19_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0797813.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            complement(23352..24065)
                     /gene="minC"
                     /locus_tag="WGR19_000211"
     CDS             complement(23352..24065)
                     /gene="minC"
                     /locus_tag="WGR19_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0797814.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            complement(24212..24580)
                     /gene="rplQ"
                     /locus_tag="WGR19_000212"
     CDS             complement(24212..24580)
                     /gene="rplQ"
                     /locus_tag="WGR19_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0797815.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            complement(24604..25590)
                     /gene="rpoA"
                     /locus_tag="WGR19_000213"
     CDS             complement(24604..25590)
                     /gene="rpoA"
                     /locus_tag="WGR19_000213"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0797816.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            complement(25616..26236)
                     /gene="rpsD"
                     /locus_tag="WGR19_000214"
     CDS             complement(25616..26236)
                     /gene="rpsD"
                     /locus_tag="WGR19_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0797817.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            complement(26256..26651)
                     /gene="rpsK"
                     /locus_tag="WGR19_000215"
     CDS             complement(26256..26651)
                     /gene="rpsK"
                     /locus_tag="WGR19_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0797818.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            complement(26671..27033)
                     /gene="rpsM"
                     /locus_tag="WGR19_000216"
     CDS             complement(26671..27033)
                     /gene="rpsM"
                     /locus_tag="WGR19_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0797819.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            complement(27099..27212)
                     /gene="rpmJ"
                     /locus_tag="WGR19_000217"
     CDS             complement(27099..27212)
                     /gene="rpmJ"
                     /locus_tag="WGR19_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0797820.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            complement(27233..27451)
                     /gene="infA"
                     /locus_tag="WGR19_000218"
     CDS             complement(27233..27451)
                     /gene="infA"
                     /locus_tag="WGR19_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0797821.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            complement(27456..28766)
                     /gene="secY"
                     /locus_tag="WGR19_000219"
     CDS             complement(27456..28766)
                     /gene="secY"
                     /locus_tag="WGR19_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0797822.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            complement(28778..29212)
                     /gene="rplO"
                     /locus_tag="WGR19_000220"
     CDS             complement(28778..29212)
                     /gene="rplO"
                     /locus_tag="WGR19_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0797823.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            complement(29214..29399)
                     /gene="rpmD"
                     /locus_tag="WGR19_000221"
     CDS             complement(29214..29399)
                     /gene="rpmD"
                     /locus_tag="WGR19_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0797824.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            complement(29392..29910)
                     /gene="rpsE"
                     /locus_tag="WGR19_000222"
     CDS             complement(29392..29910)
                     /gene="rpsE"
                     /locus_tag="WGR19_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0797825.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            complement(29929..30282)
                     /gene="rplR"
                     /locus_tag="WGR19_000223"
     CDS             complement(29929..30282)
                     /gene="rplR"
                     /locus_tag="WGR19_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0797826.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            complement(30296..30829)
                     /gene="rplF"
                     /locus_tag="WGR19_000224"
     CDS             complement(30296..30829)
                     /gene="rplF"
                     /locus_tag="WGR19_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0797827.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            complement(30843..31235)
                     /gene="rpsH"
                     /locus_tag="WGR19_000225"
     CDS             complement(30843..31235)
                     /gene="rpsH"
                     /locus_tag="WGR19_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0797828.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            complement(31251..31556)
                     /gene="rpsN"
                     /locus_tag="WGR19_000226"
     CDS             complement(31251..31556)
                     /gene="rpsN"
                     /locus_tag="WGR19_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0797829.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            complement(31559..32098)
                     /gene="rplE"
                     /locus_tag="WGR19_000227"
     CDS             complement(31559..32098)
                     /gene="rplE"
                     /locus_tag="WGR19_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0797830.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            complement(32108..32431)
                     /gene="rplX"
                     /locus_tag="WGR19_000228"
     CDS             complement(32108..32431)
                     /gene="rplX"
                     /locus_tag="WGR19_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0797831.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            complement(32443..32811)
                     /gene="rplN"
                     /locus_tag="WGR19_000229"
     CDS             complement(32443..32811)
                     /gene="rplN"
                     /locus_tag="WGR19_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0797832.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(33033..33296)
                     /gene="rpsQ"
                     /locus_tag="WGR19_000230"
     CDS             complement(33033..33296)
                     /gene="rpsQ"
                     /locus_tag="WGR19_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0797833.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            complement(33296..33487)
                     /gene="rpmC"
                     /locus_tag="WGR19_000231"
     CDS             complement(33296..33487)
                     /gene="rpmC"
                     /locus_tag="WGR19_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0797834.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            complement(33487..33903)
                     /gene="rplP"
                     /locus_tag="WGR19_000232"
     CDS             complement(33487..33903)
                     /gene="rplP"
                     /locus_tag="WGR19_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0797835.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            complement(33887..34579)
                     /gene="rpsC"
                     /locus_tag="WGR19_000233"
     CDS             complement(33887..34579)
                     /gene="rpsC"
                     /locus_tag="WGR19_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0797836.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            complement(34589..34918)
                     /gene="rplV"
                     /locus_tag="WGR19_000234"
     CDS             complement(34589..34918)
                     /gene="rplV"
                     /locus_tag="WGR19_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0797837.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            complement(34927..35205)
                     /gene="rpsS"
                     /locus_tag="WGR19_000235"
     CDS             complement(34927..35205)
                     /gene="rpsS"
                     /locus_tag="WGR19_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0797838.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            complement(35212..36045)
                     /gene="rplB"
                     /locus_tag="WGR19_000236"
     CDS             complement(35212..36045)
                     /gene="rplB"
                     /locus_tag="WGR19_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0797839.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            complement(36051..36365)
                     /gene="rplW"
                     /locus_tag="WGR19_000237"
     CDS             complement(36051..36365)
                     /gene="rplW"
                     /locus_tag="WGR19_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0797840.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLASATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRRDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            complement(36362..36982)
                     /gene="rplD"
                     /locus_tag="WGR19_000238"
     CDS             complement(36362..36982)
                     /gene="rplD"
                     /locus_tag="WGR19_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0797841.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            complement(36982..37626)
                     /gene="rplC"
                     /locus_tag="WGR19_000239"
     CDS             complement(36982..37626)
                     /gene="rplC"
                     /locus_tag="WGR19_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0797842.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGISKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            complement(37890..38195)
                     /locus_tag="WGR19_000240"
                     /pseudo
     CDS             complement(37890..38195)
                     /locus_tag="WGR19_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(38316..38627)
                     /gene="rpsJ"
                     /locus_tag="WGR19_000241"
     CDS             complement(38316..38627)
                     /gene="rpsJ"
                     /locus_tag="WGR19_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0797843.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(38645..>38872)
                     /locus_tag="WGR19_000242"
     CDS             complement(38645..>38872)
                     /locus_tag="WGR19_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0797844.1"
                     /translation="HTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVEL
                     IAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT        10205 a        10724 c         8836 g         9107 t
ORIGIN      
        1 aacgccgtac tggtttttgt taatccacta taaatgacga tataagtatt tttattttaa
       61 tccgccatat taacgcaccc ggccaaacag cataaaggca cgggcagccc gatttggatt
      121 gacctatttc cccgacaccc tcgccatcag ctcgtccgcc aaggcaagat acgccttggt
      181 accctttgcc tgcgcgtcgt aagccatcac cggcataccg tggctcggtg cttctgcaag
      241 gcggatattg cgcgggatga cggtttcaaa aagcaaatcc ccgaaatggc tgcgcaactg
      301 ttcgctgact tcggcaacca gcctgctgcg gctgtcgtac atcgtgcgca cgatgcccgt
      361 gatgtccaaa tcgggattga ccgcctgacg gattttacgc acggtcgcaa tcaaatcgga
      421 aatcccctca agcgcgtaat attcgcacaa catcggcaca atcacgccgc ctgccgccac
      481 caagccgtta agcgtcaaca gcgtcagcga aggcgggcag tctatcagga taaagtcgta
      541 atcttccgcc actgccttga gtgcgttttt caaacgcact tcccgggcga tttcctgcac
      601 cagctcgatt tccgcaccgg ccagcgcgcg gttcgcaccc aacacagcgt atccgccctc
      661 tttgctgcgt accgccgccg atttcacgtc cgcatcgccc aataagacct gataaacgcc
      721 ggactgcaaa ctcgccttgt cgatgccgct gcccgtcgtc gcattgccct gcggatccaa
      781 atcgaccacc agcacgcgtt tgccgcgcga tgccagcgaa gccgccaaat ttaccgtcgt
      841 cgtcgttttg cccacaccgc ccttctgatt ggcgatggca aggatgttcg cactcatgtc
      901 ccgcccgtgt cccgtctgat gttgaaaaaa gaaaaacagt gcggaatttt acctttatcc
      961 gcacaaaaaa ggtattcatg ccgtctgaag tgcgctcaac gcttgctcag gatgacgata
     1021 tggcgttccg catccaagcc cggcacgtcg agcctttgga ctttttcaac gcacacatcc
     1081 tgcggcaggc ggccgatttc cccctgcgga tacacgccct tcatcgccgc ccagtatccg
     1141 ccgtctttca acagatgcac cgtccacgac acaaaatccg ccagttctgc aaacgcacgg
     1201 ctggtaacca cgtcggcacg cacgtccgaa accgcctcca cgcgtccgga taccacgcgc
     1261 acattgtcca atcccaactc gataaccgcc tgctgtaaaa aagccgtttt cttcgtattc
     1321 gcatccaaaa gggttatctg cacatccgga cggcacaccg ccgccggaat gccgggctga
     1381 ccgccgcccg aaccgacatc cagcatcgtt tgcacaccct cgatatgggg cagcagcgtc
     1441 aggctgtcca aaagatgatg gacaatcatt ttttcctcgt cgcgcagggc ggtcagattg
     1501 taggttttgt tccacttttt caacaaatcc acataggcca aaagcctgtc ctgcgccgtt
     1561 tccgaaatat ccagccccat cgcggcaatg cctgcacgca ggcgttcttt gcgttccata
     1621 tctgcttcct gtccatattc aggcgtaatg ttagcggaaa aacatgccat ctgaaaccgc
     1681 gtgtcggggt tcagacggca tttcccgttc caacctattt tttcctgtga aacaccaccg
     1741 gctgcaccgg cgcgcgcgtc aggcgtttgg gcagcgtcag catagacacc agccacacgg
     1801 caaacgcata ttccgacacc gcctccccgc gcagcccgcc caaggcggca ctgcccaaca
     1861 acagctcgaa aatccacgcg accgcagcag ccgcgcaagc cgccacgccc gacagaaaca
     1921 gcgcgtaacg cgaatgccgc cgcatcatct ttttgtacgc cagtttcgtc agacccgccg
     1981 acacgactag gttcaaaaac gcaaacacgc catacacaaa ccaaacagtg ccgtcccaac
     2041 cgccacgcca gccgaatccg ccgtccgaca ccgacaccag cagccccgcc cgtccgaacg
     2101 caagcgcaaa ggcatcatgc accgccaacg caaacggcag cgtcaaaaac accagtcccg
     2161 caaacagagc cgcataagga tctttctcgt tcacaaacat cgccgtcccc ctcttccgaa
     2221 gcagaccgca ttataccgcg cactgccttg ccgcccgcta gaaaagttgc acaaacaacc
     2281 gttcatatat atcataacga aaaacgccgg tgtagctcag tcggtagagc agcgcattcg
     2341 taacgcgaag gtcgggggtt cgattccctt ctccggcacc aataccaagc acagaccctc
     2401 ccttcctcgg gaagcctgtg ctttttcaca tttccgcttc agacggcaac cgatatgaac
     2461 acctcgcaac gcaaccgcct cgtcagccgc tggctcaact cctacgaacg ctaccgccac
     2521 cgccgcctca tacacgccgt gcggctcggc ggggccgtcc tgttcgccac cgcactcgcc
     2581 cggctgctcc acctccaaca cggcgaatgg ataggcatga ccgtcttcgt cgtcctcggc
     2641 atgctccagt ttcaaggggc gatttactcc aaggcggtgg aacgtatgct cggcacggtc
     2701 atcgggctgg gcgcgggttt gggcgtttta tggctgaacc agcattattt ccacggcaac
     2761 ctcctcttct acctcaccgt cggcacggca agcgcactgg ccggctgggc ggcggtcggc
     2821 aaaaacggct acgtccctat gctggcgggg ctgacgatgt gcatgctcat cggcgacaac
     2881 ggcagcgaat ggttcgacag cggcctgatg cgcgcgatga acgtcctcat cggcgcggcc
     2941 atcgccatcg ccgccgccaa actgctgccg ctgaaatcca cactgatgtg gcgtttcatg
     3001 cttgccgaca acctgaccga ctgcagcaaa atgattgccg aaatcagcaa cggcaggcgc
     3061 atgacccgcg aacgcctcga agagaacatg gcgaaaatgc gccaaatcaa cgcacgcatg
     3121 gtcaaaagcc gcagccacct cgccgccaca tcgggcgaaa gccgcatcag ccccgccatg
     3181 atggaagcca tgcagcacgc ccaccgcaaa atcgtcaaca ccaccgagct gctcctgacc
     3241 accgccgcca agctgcaatc tcccaaactc aacggcagcg aaatccggct gcttgaccgc
     3301 cacttcacac tgctccaaac cgacctgcaa caaaccgtcg cccttatcaa cggcagacac
     3361 gcccgccgca tccgcatcga taccgccatc aaccccgaac tggaagccct cgccgaacac
     3421 ctccactacc aatggcaggg cttcctctgg ctcagcacca atatgcgtca ggaaatttcc
     3481 gccctcgtca tcctgctgca acgcacccgc cgcaaatggc tggatgccca cgaacgccaa
     3541 cacctgcgcc aaagcctgct tgaaacacgg gaacacggct gacggtcgga cacgatgccg
     3601 tctgaaacgc ctgccgcttc agacggcatc tccaatccgc ctgccttgtc gtcatgcccg
     3661 aataacggta ctattcgcag ttaacagttt tcatgccgtc tgaaaggttt gaagcgcgtt
     3721 tcagacggct gcccgcctac cttatcttac cgaaagaaca atatgatcaa cgatattcaa
     3781 aaaacagccg aaggcaaaat gcagcgttcg gtcgaagtac tgaaagaaaa tctggcgaaa
     3841 gtgcgtaccg gccgcgcgca taccggcctg ctcgaccaag tggaagtcga atactggggc
     3901 agcatggtcc ccgtcagcca agttgccaac gtaacgcttc tggacgcgcg caccatcggc
     3961 gtgaaaccgt ttgagggcaa tatggcggcc aaagtcgaga aagccatccg cgattcaaac
     4021 ttggggctga acccggcagc tgtcggcgac ctgatccgcg tgccgatacc catgctgacc
     4081 gaggaacgcc gcaaagacct gattaaagtc gtacgcggcg aagcggaaga aggccgcgtc
     4141 tccatccgca acgtgcgccg cgatgccaac gaccacatca aaaaactcct caaagacaaa
     4201 gaaatttccg aagacgaggc acgtcgcggc gaagaagcgg ttcaaaaact gaccgacaaa
     4261 tacattaccg aagccgacaa actcctgact gccaaagaag aagatttgat ggcaatttaa
     4321 cctgcacggt tcggcgttca gacggcattt gaatgccgaa ccgcgaaagg cagacatgaa
     4381 aagcagcacg caggccgttt tggaacacac cgccattccc aagcatatcg ccgtgattat
     4441 ggacggcaac ggccgttggg cgaaaaaacg tttcctccca cgcataatgg gacacaaacg
     4501 cggtttggac gcattggaaa atatggtgaa gcattgcgcc aaactgggtg tgcaatatct
     4561 gaccgtgttt gccttttcaa ccgaaaactg gcgccgcccc gaagacgaag tttcgttcct
     4621 gatggggctg tttttacagg ctttgcaaaa acaggtacgc cgcctgcacg aaaacaatat
     4681 gcgcctgaaa atactgggca gccgcgaacg ctttaatcgg cagattctgc aaggcatcga
     4741 agaggcggaa gccttgacgg caaacaatac cggcctgacc ctgagcattg ccgccgatta
     4801 cggcggacgt tgggacattt tgcaggcggc aaacaaactg attgccgaag gcgtatccga
     4861 gattacggaa gacacgctgg cgaaacactt gatgctgggc gatgcaccgg aaccggattt
     4921 gttcatccgc accggcggcg aaacgcgcat cagcaatttc ctgctctggc agatggcata
     4981 tgcagaactg tatttcaccg atattttgtg gcccgatttt gacggcaagg ctttggacga
     5041 tgccgtcgct tcgttccaaa aacgcgaacg gcggttcgga cgcacctccg agcaactgcc
     5101 tatcgaacag caaaggaact aaattatgct gaaacaacgg gtaataaccg ccatgtggct
     5161 gctgccgctg atgctgggca tgctgtttta cgcgccgcaa tggctgtggg ctgcattttg
     5221 cggactgatt gccctgattg ccttgtggga atatgcccgt atgagcggct tgtgcaaaat
     5281 caaaaccaac cattacctcg ccgcaacctt ggttttcggc gtggttgcct atgcgggcgg
     5341 ctggatgctg cctaatttgg tttggtatgt tgttttggca ttttggctcg ccgttatgcc
     5401 tttatggttg agattcaaat ggaggctcaa cggcggttgg caggtttatg ccgccggctg
     5461 gcttctggtc atgccgtttt ggttcgcgct cgtatccctg cgcccgcatc ccgatgatgc
     5521 cctgccgctg ctcgccgtga tgggtttggt gtgggttgcc gatatttgcg cgtatttcag
     5581 cggcaaggcg ttcggcaaac acaaaatcgc accggcaatc agccccggca aaagctggga
     5641 aggtgcaatc ggcggcgcgg tttgcgtggc cgtgtacatg accgccgtac gaagtgccgg
     5701 ctggctggca ttcgatacag gctggttcga taccgtgtta atcggtttgg tgctgaccgt
     5761 tgtcagcgta tgcggcgacc tgttggaaag ctggctcaag cgcgcggcgg gcattaaaga
     5821 cagcagcaag ctgctgcccg gacacggcgg cgtgttcgac cgtaccgaca gcctgattgc
     5881 cgttatcagc gtctatgcgg cgatgatgtc ggttttaaat tgattctatg ccgtctgaaa
     5941 acgcttcaga cggcatccgg tataaagtta tccccattat gacaccacaa gtcctgacca
     6001 tattaggcag taccggcagc ataggcgaaa gcacgctgga cgttgtctcc ctccaccccg
     6061 aaaaattccg cgtattcgcg ctggcagggc ataagcaggt cgagaaactg gcggtgcaat
     6121 gccggacgtt ccgccccgaa tatgccgtcg ttgccgatgc cgaacacgcc gcccggcttg
     6181 aagctttgtt gaaaggcgac ggcacggcga cgcaggtttt acacggcgcg caggcattgg
     6241 ttgacgttgc ctctgccgac gaagtcagcg gtgtcatgtg cgccatcgtc ggggcggtgg
     6301 ggctgccttc cgcgctcgca gcggcgcaaa aaggcaaaac catttatctg gcaaacaaag
     6361 aaacgctggt ggtttccggc gcgttgttta tggaaaccgc ccgtgcaaac ggcgcggcag
     6421 tgctgcccgt cgacagcgaa cacaacgccg ttttccaagt tttgccgcgc gattacgccg
     6481 gccgtctgaa cgaacacggc atcgcttcga ttatcctgac cgcttccggc ggcccgtttc
     6541 tgaccgccga tttaaacacg ttcgacagca ttacgcccgc ccaagcggtc aaacacccca
     6601 attggagcat ggggcgcaaa atctccgtcg attcctccac catgatgaac aaaggtttgg
     6661 agctgattga agcgcattgg ctgttcaact gtccgcctga caagctcgaa gtcgtcatcc
     6721 atccgcaatc cgtgatacac agcatggtgc gctaccgcga cggctccgtg ttggcgcaac
     6781 tgggcaatcc cgatatgcgt acgcccatcg cctattgttt gggcttgccc gagcgcatcg
     6841 attcgggtgt cggcgacctg gatttcgaca cattgtccgc gctgaccttc caaaagcccg
     6901 acttcggccg cttcccctgc ctgaaactcg cctatgaagc catgaacgcg ggcggagtcg
     6961 cgccctgcgt attgaacgcc gccaacgaaa ccgccgtcgc cgcctttttg gacggacgga
     7021 ttaagtttac cgacattgcc aaaaccgtcg cccattgtct cgcacaagac ttttcagacg
     7081 gcataggcga tatagggggg ctcttggcgc aagatgcccg gacacgcgca caagcgcggg
     7141 catttatcgg cacactgcgc tgatgccgtc tgaacaccgt tatcaaagga aaaccatttg
     7201 cacacccttc tagcttttat cttcgccatc ctgattttgg tcagtctgca cgaattcgga
     7261 cactacgccg ttgcaaaatt atgcggcgta aaagtgctgc gtttttccgt cggcttcggc
     7321 aaaccgtttt ttacccgaaa gcgcggcgat accgaatggt gtttggcacc catccccttg
     7381 ggcggctatg tcaaaatggt cgatacgcgc gaaggcgaag tatcagaagc cgatttaccc
     7441 tacgcttttg acaaacaaca ccccgccaag cgcatcgcca tcgtcgccgc cggcccactg
     7501 accaacctcg cactggcggt tttgctgtac ggactgagct tttccttcgg cgtaaccgaa
     7561 ctgcgcccct acgtcggcac agtcgaaccc gacaccattg ccgcccgcgc cggcttccaa
     7621 agcggcgaca aaatacaatc cgtcaacggc acacccgttg cagattgggg cagcgcgcaa
     7681 actgaaatcg tcctcaacct cgaagccggc aaagtcgccg tcggcgttca gacggcatcg
     7741 ggcgcgcaaa ccgtccgcac catcgatgcc gcaggcacgc cggaagccgg taaaatcgca
     7801 aaaaaccaag gctacatcgg actgatgccc tttaaaatca caaccgttgc cggtggcgtg
     7861 gaaaaaggca gccccgccga aaaagcaggc ctgaaaccgg gcgacaggct gactgccgcc
     7921 gacggcaaac ccatcgcctc atggcaagaa tgggcaaacc tgacccgcca aagccccggc
     7981 aaaaaaatca ccctgaccta cgaacgcgcc ggacaaaccc ataccgccga catccgcccc
     8041 gatactgtcg aacagcccga ccacaccctg atcgggcgcg tcggcctccg tccgcagccg
     8101 gacagggcgt gggacgcgca aatccgccgc agctaccgtc cgtctgttgt ccgcgcattc
     8161 ggcatgggct gggaaaaaac cgtttcccac tcgtggacaa ccctcaaatt tttcggcaaa
     8221 ctaatcagcg gcaacgcctc cgtcagccat atttccggtc cgctgaccat tgccgatatt
     8281 gccggacagt ccgccgaact cggcttgcaa agttatttgg aatttttggc actggtcagc
     8341 atcagcctcg gcgtgctgaa cctgctgccc gtccccgttt tggacggcgg gcacctcgtg
     8401 ttttatactg ccgaatggat acgcggcaaa cctttgggcg aacgcgtcca aaacatcggt
     8461 ttgcgcttcg ggctcgccct gatgatgctg atgatggcgg tcgccttctt caacgacgtt
     8521 acccggctgc tcggttagat tttacgtttc ggaatgccgt ctgaaaccgc attccgcacc
     8581 acaaggaact gacaatgaaa ctgaaacaga ttgcttccgc actgatggtc ttgggcatat
     8641 cgcctttggc acttgccgac ttcaccatcc aagacatccg cgtcgaaggc ttgcagcgta
     8701 ccgagccgag taccgtattc aactacctgc ccgtcaaagt cggcgatacc tacaacgaca
     8761 cacaaggcag tgccatcatc aaaaacctgt acgccaccgg tttctttgac gacgtacgcg
     8821 tcgagactgc ggacgggcag ctcctgctga ccgtcatcga acgccccacc atcggatcgc
     8881 tcaatatcac cggcgcaaaa atgctgcaaa acgacgccat caagaaaaac ctcgaatcgt
     8941 tcgggctggc gcagtcgcaa tactttaatc aggcgacact caatcaggca gtcgccggac
     9001 tgaaagaaga atacctcgga cgcggcaaac tcaatatcca aatcacgccc aaagtgacca
     9061 aactcgcccg caaccgcgtc gacatcgaca tcacgattga cgagggcaaa tccgccaaaa
     9121 tcaccgacat cgaatttgaa ggcaaccaag tctattccga ccgcaaactg atgcggcaga
     9181 tgtcgctgac cgaaggcggc atttggacat ggctgacacg aagcaaccaa ttcaacgagc
     9241 agaaatttgc ccaagacatg gaaaaagtaa ccgacttcta ccaaaacaac ggctacttcg
     9301 atttccgcat cctcgatacc gacatccaaa ccaacgaaga caaaaccaag cagaccatca
     9361 aaatcaccgt ccacgaaggc gaacgtttcc gttggggcaa agtctccatc gaaggcgaca
     9421 ccaacgaagt ccccaaagcc gaactggaaa aactgctgac catgaagccc ggcaaatggt
     9481 acgaacgcca gcagatgacc gccgttttgg gtgagattca gaaccgcatg ggctcggcag
     9541 gctacgcata cagcgaaatc agcgtacagc cgctgccaaa cgccgaaacc aaaaccgtcg
     9601 atttcgtcct gcacatcgaa ccgggccgga aaatctacgt caacgaaatc cacatcaccg
     9661 gcaacaacaa aacccgcgac gaagtcgtgc gccgcgaatt gcgccaaatg gaatccgcgc
     9721 cttacgacac ctccaagctg caacgctcca aagagcgcgt cgagcttttg ggctacttcg
     9781 acaatgtcca gtttgacgcc gtcccgcttg ccggcacgcc cgacaaagtc gatttgaaca
     9841 tgagcctgac cgaacgttcc accggttccc tggatttgag cgcgggttgg gtacaggata
     9901 ccggcctggt catgtccgca ggcgtttccc aagacaacct gttcggtacg ggcaagtcgg
     9961 ccgccctgcg cgcctcacga agcaaaacca cgctcaacgg ctcgctgtcg tttaccgacc
    10021 cgtacttcac ggcagacggg gtcagcctgg gctacgatgt ttacggaaaa gccttcgacc
    10081 cgcgcaaagc atcgaccagc atcaaacaat ataaaaccac cacggcaggc gcaggcatcc
    10141 gcatgagcgt gcctgttacc gaatacgacc gcgtgaattt cggtttggtg gcagaacacc
    10201 tgaccgtcaa cacctacaac aaagcgccca aacactatgc cgactttatc aaacaatacg
    10261 gcaaaaccga cggcacagac ggcagcttca aaggctggct gtacaaaggt accgtcggct
    10321 gggggcgcaa caaaaccgac agcgcgttat ggccgacgcg cggctacctg acgggcgtga
    10381 acgccgaaat cgccctgccc ggcagcaaac tgcaatacta ctccgccacc cacaaccaaa
    10441 cctggttctt ccccctgagc aaaaccttca cactgatgct tggcggcgaa atcggctatg
    10501 ccaacggcta cggcaaaacc aaagaaatcc ccttctttga aaacttctac ggcggcggcc
    10561 tgggttcggt acgaggttac gaaagcggca cactcggtcc gaaagtctat gacgaatacg
    10621 gcgaaaaaat cagctacggc ggcaacaaaa aagccaacgt ctccgccgag ctgctcttcc
    10681 cgatgcccgg cgcgaaagac gcgcgcaccg tccgcctgag cctgtttgcc gacgcaggca
    10741 gcgtgtggga cggcaaaacc tacgacgaca acagcagttc cgcgaccggc ggcagggttc
    10801 aaaacattta cggcgccggc aatacccata aatccacctt taccaacgaa ttgcgctatt
    10861 ccgccggcgg cgcggttacc tggctctcgc ctttaggccc gatgaaattc agctacgcct
    10921 acccgctgaa gaaaaaaccg gaagacgaaa tccaacgctt ccaattccaa ctcggcacga
    10981 cgttctaatc ccgcaaatgc cgtctgaagc ccttcagacg gcatttcgcg gcaacattcg
    11041 aaggagtttt accatgaccc gtttgacccg cgcgtttgcc gcggctctga tcggtttgtg
    11101 ctgcaccgca ggcgcgcacg ccgacacctt ccaaaaaatc ggctttatca ataccgagcg
    11161 catctacctc gaatccaagc aggcgcgcaa gattcaaaaa acgctggaca gcgaattttc
    11221 cgcccgtcag gacgaattgc aaaaactgca acgcgaaggt ctggatttgg aaaggcagct
    11281 tgccgaaggc aaactcagaa acgcaaaaaa ggcgcaagcc gaagaaaaat ggcgcgggct
    11341 ggtcgcagcg ttccgcaaaa aacaggcgca gtttgaagaa gactacaacc tccgccgcaa
    11401 cgaagagttt gcctccctcc agcaaaacgc caaccgcgtc atcgtcaaaa tcgccaaaca
    11461 ggaaggttac gatgtcattt tgcagaacgt gatttacgtc aacacccaat acgacgttac
    11521 tgacagcgtc attaaagaaa tgaacgcccg ctgacccttt cagacggcat accgaacagg
    11581 aaaaccatga ttccggccac ctacaccctg tcccaaatca ccgcgcggct cggcggcgaa
    11641 tggcgcggcg aggacatttc cgttaccacc gtgcgcccgc tcgcagacgc gcaggcggaa
    11701 cacatcagct tcctcgccaa tccgaaatac aaagccgaag tccacgacag cagcgcgggc
    11761 gcggtcatcg tttccgccaa agcggcagac ggatttgaag ggcgcaacct gattgtcgcc
    11821 gacgacccct atctctattt cgccaaagtc gcccgcctgt tttcacccgt cgtcaaagcg
    11881 cgcggcggca tccatccgac cgccgtcgtc gaaccgggcg cgaccgttcc cgccagctgc
    11941 gaaatcggcg cgaacgtcta catcggcgca aacaccgtgc tcggcgaagg ctgccgcatc
    12001 ttggcaaacg ccgtcgtcca acacgattgc aaactgggcg acgaagtcgt cctgcatccc
    12061 aacgccgtcg tttattacgg ctgtacactg ggcagacgcg tcgaaatcca cagcggcgcg
    12121 gtcatcggcg cggacggttt cggactcgct tttgccggcg attcatggtt taaaatcccg
    12181 caaaccggcg cggtaacgct gggcgacgac gtggaaatcg gctcgaacac caacatcgac
    12241 cgcggcgcga tgagcgacac caccgtcggc aacggcacca aaatcgacaa ccaagtccaa
    12301 atcggacaca actgcaaaat cggttcgcac accgtcatcg ccgccaaaac cggcatctca
    12361 ggcagcgtaa ccataggcag ctactgcatc atcggcggcg gcgtcggtac ggtcggacac
    12421 atcgaaatcg ccgacaaaac caccatcggc ggcggcacgt ccgtcaccca cagcattacc
    12481 gaaagcggca aacacctcgc cggcatcttc ccgatgtcca cccataaaga atgggcgcgc
    12541 aacgctgttt acatccaccg cttaagcgaa atgaacaaac ggctcaaaac actagagcag
    12601 cagctttcag ataccggtca agacagcaaa taaccaaacc gactttattc aaggaatacg
    12661 acagacatgg acgtacaact ccccatcgaa gccaaagaca tccaaaaact catcccccac
    12721 cgctatccgt ttctccagct cgaccgcatc accgccttcg agccgatgaa aaccctgacc
    12781 gcgattaaaa acgtcaccat aaacgaaccc cagttccaag gccatttccc cgacctgccc
    12841 gtgatgcccg gcgtactcat catcgaagcg atggcgcagg cgtgcggcac gttggcgatt
    12901 ttgagcgaag gcgggcgcaa agaaaacgaa ttcttcttct tcgccggcat agacgaagcc
    12961 cgtttcaaac gccaagtcat ccccggcgac caactcgtct ttgaagtcga gctgctgacc
    13021 agccggcgcg gcatcggcaa attcaacgcc gttgccaaag tggacgggca agtcgccgtc
    13081 gaagccatca tcatgtgtgc caaacgcgta gtttgagtgt tcagaaaaag gtcgtctgaa
    13141 agttttcaga caacctgttg ccgtcgcgca tcttcgcggc aacacgacag gaaaggaaaa
    13201 acatgaccct catccacccg accgccgtca tcgaccccaa agccgaactc gactccggcg
    13261 tcaaagtcgg cgcgtacacc gtcatcggcc ccaacgtcca aatcggcgcg aataccgaaa
    13321 tcggcccgca cgccgtcatc aacggccaca ccagcatcgg cgaaaacaac cgcattttcc
    13381 aatttgccag cctcggcgaa atcccgcagg acaaaaaata ccgcgacgag ccgaccaagc
    13441 tgattatcgg caacggcaac accatccgcg aattcaccac ctttaattta ggcacggtaa
    13501 ccggcatcgg cgaaacccgt atcggcgacg acaactggat tatggcgtac tgccacctcg
    13561 cgcacgactg cgtggtcggc aaccacacca tcttcgccaa caacgcctcg cttgccggac
    13621 acgttaccat cggcgactac gtcgttttgg gcggctacac gctggttttc caattctgcc
    13681 acatcggcga ctacgctatg accgccttcg ccgcaggcgt acacaaagac gtaccgccct
    13741 actttatggc atcgggctac cgcgccgaac cggcggggct caacagcgaa ggcatgcgcc
    13801 gcaacggttt taccgcagaa cagatttccg ccgtcaaaga cgtgtacaaa accctctacc
    13861 atcgcggcat tccgtttgaa gaggccaagg cagacatcct ccgccgcgcc gaaacccaag
    13921 ccgagcttgc cgtattccga gactttttca cacaatcgac gcgcggcatc atccgctgac
    13981 cgtacccttt tgatgccgtc tgaaaccccg aaagcgtttc agacggcatc ggttttttta
    14041 aagcggtata ttgtttgatc gatgcatcat tttgtagatt tcagaagtat ggcagtcggc
    14101 agcattgatg atttctgccg cccgccacat ccctattcgg acatttcatt gtgacctccg
    14161 ccgctgcttt taaagaattg caaacaattt ccgcagcttg aaccggcctg ttttcggcga
    14221 aaaaaaccaa aagtattttt caaaagaaca tattgtctta ttggatattc tgtgctgaaa
    14281 ttactggaca ctacaaaagt ggtgttttaa aaataaaaca gacaaaggag aaagccatgc
    14341 aagtgttttt agacaatccc aaagcctttt ttgaaaccgt cagcggctgg gtttggggac
    14401 ctttgatgct gatgctgctg gtcggcacgg gtattttgct gactgtttta ttaaaaggtt
    14461 tgcagttcac gatgttgggt tatgcgctga aacaggcgtt tatgccgcca aagaagcata
    14521 aaagcggcga aggccacgaa ggcgatattt cccatttcgc ggcgttgatg accgcgctgt
    14581 ccgccaccat cggtacgggt aacatcgccg gcgtggcgac tgcggtggta accggcggcc
    14641 cgggcgcggt attttggatg tggatgaccg ccattttcgg catggccacc aaatacggcg
    14701 aaggcgtgtt ggcggtgaaa taccgcgtca acaattccaa aggcgaaatg tccggcggcc
    14761 cgatgtatta catcgaaaaa ggcttgggca aaaactggaa atggatggcc gttgcatttg
    14821 cgctgttcgg cacattcgct tccttcggta tcggcagctc ggtgcagtcc aactcggttg
    14881 cacaggcggt gcaaaccagc ttcggtatcg aacctgccta taccggcatt acgttgaccg
    14941 ttctgactgc cgttgtcgtt ttaggtggta ttaaaggcat cgccaaagcc gcttctttca
    15001 tcgtgcctgc tatggcggtg ttttatgtgt tgggcggtct ttccattatc gcgattaatt
    15061 ccgatgcact gatgcctgcc gtcaagctga ttttctccga tgcgttcagc gcgcaggcag
    15121 tggcgggcgg tgctatcggt acggtcatcc gctacggcgt ggcgcgcggc gtgttctcca
    15181 atgaggcggg tatgggttct gcgcctattg ccgccgcagc cgcgaaaacc gaccatcccg
    15241 tccgtcaggc cttggtttct atgaccggta cgtttttgga taccatcgtc gtttgctcga
    15301 ttaccggcat cgtgttggta atgggactgc tcggcgcggg cggcgagttt gtcaaacctg
    15361 aagtgagcgg cgcggcactg acaaccgtca cattccaaaa aatgctgccc ggcatcggcg
    15421 gctggattgt caccatcggc ctgattttct ttgcctactc aaccattctc ggctggtgct
    15481 attacggcga aaaatgcgcg gtgtatgttt tcggcgagaa atttgccggt ttgtaccgcg
    15541 tcggttatgt ttcttccgtt atgctgggta ccgtgttgag ccttgatttg gtgtggctgg
    15601 cttcggacac attcaacggc ttgatggcat tgcccaacct gattgcactt ttattgatgg
    15661 cgaaagtcat cgtcaatgaa acgcgcgact tcaaacaaaa aatcacaaac ggcgaattgc
    15721 cgcattaata tccgaggccg tctgaatccg aatattcaga cggcacggca tattaagtct
    15781 gttttgaaaa agtcggaggg agaaacatga aagtgcttgt tttaggtgcg ggtgttgccg
    15841 gcgtatcttc cgcgtggtat ctggcagagg caggacatga agtaacggtc atcgaccgcg
    15901 ccgagggcgt ggcgatggaa accagttttg ccaatgcagg tcagctttct tacggctata
    15961 ccacgccttg ggctgcaccc ggtattccga ccaaagcact gaaatggctg tttaaaagcc
    16021 atccgccttt gctgtttcgc cccgacggca gcctgtatca aatcgaatgg ctgtggcgga
    16081 tgctgcaaaa ctgcacggca gcgcactatc aaaccaataa agagcgcatg gtcaggattt
    16141 ccgaatacag ccgtgaaatg ttccgccgtt ttgaagcgca aaccggcatg aattttgaag
    16201 ggcgcaaaaa agggacgttg cagattttcc gccaaaccaa agaagtcgaa gcggcagaac
    16261 aagacattgc cgttttggaa cgctacggcg tgccgtaccg ccgtctgaaa cccgaagaat
    16321 gtgcagaatt tgagcctgcg ctggcacgcg ttaccgccaa aattgccggc ggcctgcacc
    16381 tgcctgcaga tgcgaccggc gactgccacc tcttcactga aaacctatac aaattgtgtc
    16441 aggaaaaggg cgtacagttt catttcaacc aaaccatcag ccgcatcgac cataacgggc
    16501 tgcgcatcaa agccgttgaa accgaaacag ggcggtttga agcagatgcc gttgtctgtg
    16561 cgctcggctg cttcagtagg actgtgttgg cgcagttaga cctcgatctg ccgatttatc
    16621 ccgtcaaagg ctattccttg accctgccgg tcaccaattc agacggcgcg ccggtgtcca
    16681 ccgttttgga tgaaagctac aaagtcgcca tcacgcgctt tgacaacaga atccgcgtcg
    16741 gcggtatggc ggaattgtcg ggctacgaaa tcaaactgcc cgaaaaacgc cgcgaaacct
    16801 tggctttggt cgtcaacgac ttgttcccgg aaggcggcga tttgagccaa gcatcgttct
    16861 ggagcggcct cagaccgatg acgcccgaca gcacgccgtt aatcggtcgc acccgctttg
    16921 acaacctgtt tctgaatacc ggccacggta ctttaggctg gaccatgtcg ctgggttcgg
    16981 caaaattaac cgccgatatc gtgagcggca aagacaccga aatccgcagc gacgatttga
    17041 gcctgtcgcg ttatcaggct taagctggaa agcactaagg tttagcggca tttcaaattt
    17101 aaaggccgtc tgaaaatcat ttttcagacg gccttttgtt tcaaaatccg tgtatccaat
    17161 caataaaata aataataact attatcatat acattaaaaa gtgttagtct gaacctgtcc
    17221 cagcttttag cgcaggtttt ctcatgcccg atttttcgat gtccaatttg gccgttgcct
    17281 tttccatcac attggctgcc ggtttgttta ccgtattagg cagtggcttg gtgatgtttt
    17341 ccaaaacgcc caatccgcgt gtgttgtcgt ttggtttggc gtttgccggc ggtgcgatgg
    17401 tatatgtttc cctgacggag attttcagta agtccagcga ggcgttcgct gaaatttatg
    17461 ataaagacca cgcgtttgcg gcggcgacca tggcattttt ggccgggatg ggcggcattg
    17521 cgctgattga ccgtctggtg ccgaacccgc atgaaacttt agacgcgcaa gacccgtcgt
    17581 ttcaagaaag caaacgccgc catatcgcgc gagtcggcat gatggcggcg tttgcgatta
    17641 ctgcgcacaa tttccccgaa ggcttggcga cgttttttgc cacattggaa aatccagcag
    17701 tcgggatgcc tttggccttg gcgattgcca tccataatat tccggagggc atttccatcg
    17761 ccgcgccggt ttattttgcc acccgcagcc gtaagaaaac ggtgtgggcg tgtctgctat
    17821 ccggcttggc cgagccgttg ggggcggctt tgggctattt ggttttgcag ccgtttttgt
    17881 cgcctgccgt gtttggttcg gtattcggcg tgatagccgg tgtgatggtg tttttggcgt
    17941 tggacgagct gctgccggct gccaaacgct attcagacgg ccatgaaacc gtttacggcc
    18001 tgacaatggg catggcggtg attgccgtca gcctggtatt gttccatttt taaaacctgt
    18061 atgccaaagg ccgtctgaaa tgattcagac ggcctttttg cgttataatc cgcacatcaa
    18121 aaataacctt aatttcacaa agttccatca tgttagacaa atacaatcca gccgaaatcg
    18181 aatccaaaca ttatcaaaac tgggaagagc agggctattt ccaacctgat atggatttga
    18241 ccaaaccgtc tttctccatc caactgccgc cgcccaatgt aaccggcacg ctgcacatgg
    18301 gtcatgcctt caaccaaacc attatggacg gcctgacccg ctactaccgt atgaaaggct
    18361 gcaacaccgc ctggattccc ggcacagacc acgcgggcat cgccacgcaa atcgtggtcg
    18421 agcgtcagct tgccgcgcaa aacgtgtccc gtcatgactt gggtcgtgaa aaattcttgg
    18481 aaaaagtgtg ggaatggaaa gaagtttccg gcggcacgat tacgcagcag atgcgccgtg
    18541 tgggctgttc tgccgactgg acgcgcgagt atttcacaat ggacgatgta cgcgccgaaa
    18601 ccgtgaccga agtgttcgta cgcctgtatg aacagggctt gatttatcgc ggcaaacgct
    18661 tggtaaactg ggatccagtg ctcggtacgg cggtgtcgga tttggaagta gaaagcgtgg
    18721 aagaacaagg ctctatgtgg cacatccgct atccgctggc ggacaatcct gccgaagccg
    18781 ttatcgtcgc caccacccgt cccgaaacgc tgttgggcga cgttgccgtt gccgtcaatc
    18841 ccgaagacga acgttatacc cacttaatcg gcaaggaatt aatcctgccg ctgaccggcc
    18901 gcaccattcc ggttatcgcc gacgaatacg ttgaaaaaga ctttggtacc ggctgcgtga
    18961 aaatcacgcc tgcgcacgac tttaacgact acgaagtcgg caaacgccac gatacgcgcc
    19021 tgattaatgt gttcgatttg gaagccaaag tgctggcaaa cgccgaagtg ttcaacttca
    19081 aaggcgaagc gcaacaaggc tttgccctgc ctgaaaaata cgcaggtttg gaccgctttg
    19141 ccgcgcgcaa acaaatggtt gccgatttgc aggaacaagg tttcttggtc gaaatcaaag
    19201 cgcacacgct gatgacgccg aaaggcgacc gtaccggttc ggtgattgag ccgatgctga
    19261 ccagccaatg gtttgtcgcc atgtccgcca cgccaaacgg cggcgagcct gacagcgaat
    19321 tcaaaggctt gagcctcgcc gacaaagcca aaaaagccgt cgacagcggc gcggtacgct
    19381 ttatccctga aaactgggtc aacacctaca accaatggat gaacaacatc caagactggt
    19441 gcatctcgcg ccaactgtgg tgggggcatc aaatccccgc gtggtacgac gaagcaggca
    19501 atgtttacgt cgcccgcaat caggtggaag ccgaaaaaca agccggcaaa acaggcttga
    19561 cccgagaaga agacgtattg gacacatggt tctcctccgc gctcgtgccg ttttccacgc
    19621 tcggctggcc gtctgaaacc gacgaactca aagccttcct gccgtccaac gtcttggtaa
    19681 ccggctacga aatcatcttc ttctgggtgg cgcgcatgat tatgatgacc acccacttta
    19741 ccggcaaagt accgtttaaa gacgtgtaca tccacggcat cgtgcgcgac cacgaaggca
    19801 aaaaaatgtc caaatccgaa ggcaacgtca tcgatcctgt ggatttgatt gacggcatcg
    19861 gcttggacaa gctgctggta aaacgtacca ccggcctgcg caaacctgaa accgcgccga
    19921 aagtggaaga agccaccaaa aaactcttcc ccgaaggcat tccgagtatg ggcgcggacg
    19981 cattgcgttt caccatggcg agttacgcca gcttggggcg cagcgtcaac ttcgacttca
    20041 aacgcgccga aggctaccgc aacttctgca ataaaatctg gaacgccacc aacttcgtct
    20101 tgatgaacac cgaaaaccaa gactgcggct acggcgcaac cgctgccgaa ccgcgcggct
    20161 attccttccc cgatatgtgg attgtaggcc gtctgaatca gaccatcgag caggttacgc
    20221 aagcctacga aacctaccgc tttgatttgg cggcagaaac cctgtacagc ttcgtatgga
    20281 acgactattg cgactggtat ttggaacttg ccaaagtgca gcttcaaacc ggctgcgcca
    20341 gccgccaacg cgccacacgc cataccttgt tgcgcgtact cgaagccgcc ctgcgcctgc
    20401 tgcacccgat tatcccgttc atcaccgaag aattgtggca aaccgtcgct cctatgtgcg
    20461 acgcgaaaac cgccgacagc atcatgctcg cccgcttccc cgaagccgac ggcggcgata
    20521 ttgttcagac ggcattcgag caaatgaccg tgttgcagga tttgattggc gcagtccgca
    20581 acctgcgcgg cgaaatgggc atccagccca atgtgaaagc cccgctgttt gtcgaaagcg
    20641 cagacgactt ggcggactac ctcaaatacc tgccgatgat gacccgactg accgaagcac
    20701 agcaagtcgc cgccctaccc gaaagcgaag acgcgcccgt cgccgtctgc aacggcgcgc
    20761 gcctgatgtt gaaagtcgaa atcgacaaag ccgccgaaac cgcccgtttg agcaaagaag
    20821 ccgagaagct gcaaaaagcc ttggacaaac tcaacgccaa actctccaaa cccggctaca
    20881 ccgaaaaagc ccctgcgcat ctggtggaaa aagacaaagc cgatttggcg gaattggaag
    20941 acaaaatggc aaaagttcaa aatcagttgg cgaagttgaa agactgattt gattgaataa
    21001 acaaggccgt ctgaaaaatg tttttcagac ggcctctttt atagtggatt aacaaaaatc
    21061 aggacaaggc gacgaagccg cagacagtac aaatagtacg gcaaggtgag gcaacgccgt
    21121 actggttttt gttaatccac tatacatggc attgcatcgg cactgatgaa aacattcaca
    21181 gtgatgcaaa agtcgaatat cttcaggacg gcagtttccg tggcgatgct atactcaaaa
    21241 tcgatgatga cggcaatatc ttggcatacc gtgttgtcgg ctcagggaaa tggcgttttg
    21301 aaaacaatgc ctgcgcctag tcgcggataa aacttacccc gtgaagctgc gactgcataa
    21361 tcgccgcctt catcgccgac aacgccttcg gacggacaaa attgcggcgg tacgccaata
    21421 cgacccgccg gcttggcggc gtaccttcaa acggaataat gctgaacagc atatgatcgt
    21481 tttcggtcag tgcggttgcc ggcaacacgc tgattgccaa accgctggca accatatggc
    21541 ggattgtatt aatcgagctg ccctgcaatg tattggtcaa cccctgtata cgttgtttcg
    21601 ccgccaattc ggaacagctt gagagtacct gatcccgcat acagttgcct tccgtcagca
    21661 gcaaaacctg ctcctcaccc agcatccggg gcgaaacggc atccagttcc tcaaatgaat
    21721 gccctttcgg gacaatcacg aaaaacggtt cgtcatacaa gggttcggta acaatgcccg
    21781 gctcttgaaa cggttcggca acgataatcg catcaacgtc cccgcgtttg agcgactcgg
    21841 tcaaagtatg cgtgtaattc tcttccaaca tcaaaggcat tttcggtgca gtacggcgca
    21901 acgagacaat cagtttcggc agcaggtacg gcgcaaccgt aaaaatcagc ccgagtttga
    21961 acgcaccctc cagctcgttt tgttcttcat ttgccaaatg cctgataagc tctgcctctt
    22021 ccaacacctt acgcgcctgt gcaacgatac gttcccccgc ctcggtcgta ataatatcgt
    22081 tgctgctccg gtcaaacaaa gagacggcaa gctcttcttc caatttctta atggcaatag
    22141 acaaagtggg ctgactgaca aaacaacgcc gcgccgccct gccgaaatga cgttcttggg
    22201 cgactgcgac gatgtaccgc aattcggtta aggtcatgtc ctataccttt ttctgttccg
    22261 gcaaagtaat gttcaactca agcacatcca taccatcctg tttttcctgg gaaatacgga
    22321 tattgtctaa tgaaacattc acatatttgg acaggacttc catcaactct ttacgtaaag
    22381 tcggcaggta atccggagcc tgaccttctt gggcgcgctc ttgggcaatg atgatttgaa
    22441 ggcggtcgcg ggcaacggtt gccgttttct gctttctgcc gaataaaaat tcgattaatg
    22501 acatacctta tcctccaaac agacgtttga agaagctttt tttctcagct tccaagaaac
    22561 gcatttcacg gttctcgccc aaaagacggg caataacgtc cttatatgcc tcggaagccg
    22621 ccacgctgtc ctgatggatg accggttctc cggaattgga tgcctgcaag acgttttggg
    22681 attcaggaat cacacccagc aaaggaatat gcagaatatc gcaaatatcc tgtacagaca
    22741 gcatttcgcc ttttgccact cgttcgggag aataacgcgt aatcaacaga tgttctttaa
    22801 ccgaaccgcc ttgctctgcc ttatgggatt tgctttgcaa aattcccaaa atcctgtcgg
    22861 agtcacgcac actggaaacc tcaggattgg tcgttacaat ggcttcatca gcaaaataca
    22921 acgccatcaa tgcaccctgc tcaataccgg caggagagtc gcaaataata tactcaaagc
    22981 ccattttctt gccggacagc tcctgcatca ctttttctac gccctcgcgt gtcaaagcgt
    23041 ctttatcccg agtctgggaa gccggcaaaa taaacaggtt ttcacaattt ttatctttaa
    23101 tcaaagcttg gttgagcgtc gcctccccct gaatgacatt gatcaggtca taaacgacac
    23161 gacgctcaca acccataatg aggtcgaggt tacgcaagcc cacatcaaaa tcaattaccg
    23221 cagttttata tccgcgtaat gccaaacctg tcgcaatact ggcactggta gtcgttttac
    23281 cgacaccgcc cttacctgaa gttactacaa taatttttgc cacaatattt cctttttaaa
    23341 atatcaaaca attactctga gccaattgca ctgataacca atcggttatc ctgcaacaat
    23401 atctgtaccg gctgcttgtg cagatggttc ggcaaatcct gttcaaaatt acggtaaata
    23461 cccgccacag aaaccagttc tgcctgcatg gagtggataa atatgcgggc agaagtatca
    23521 cccttggcac cggccaaagc acgccccctc atcggcgcat aaatatgtat attgccatcc
    23581 gcaatcaatt ccgccccctg gctgaccgcc cccgtaacaa tcaaatcgcc atcttcggca
    23641 taaacctgct gaccggtacg gacaggggat gtaatcaata ctgttttcct tgctttctga
    23701 ccatcctccg tgttctgcac ctcaacctga cccagttctt taacattttc cgaatgagac
    23761 agacaaaaca gcaaatgata cttcatagcc gcagcagccc aacgttcatt agaatgcttc
    23821 agacccaaaa tttgcatacc atgccttgaa aacaacgaaa ccaatgcagc aagatccaaa
    23881 gactcgggat aatcaaactc ttgaacatcc agcacaaatg gaacaacacc agactcttga
    23941 aacttcttgc ccaatttgac cagcacatct tccaaatcaa acaagtctga cgtatgcaaa
    24001 gatatagaca atacgtccat ctttgtcgac tttatatcga aagcattcat tatataaacc
    24061 atcatagaat tttttaatga tgtaagttta ccgcgcaaac cttagctatt caattaatat
    24121 tttaaacatg atagccaata ccctattacc tatcagatat tgcaaaaata acgccacctg
    24181 cttttcggca gatggcgtta tatgactaaa attactcagc agccggtttg tcaaccaatt
    24241 caaccagtgc cagaggtgca ttatcacctt tacggaatcc gtatttcaac acccgaacat
    24301 aaccaccgtt acgagcagta aaacgaggac ccaaatcgcc aaacagtttt actacaacat
    24361 cacggtcgcg agtacggtca aatgccaaac ggcggtttgc caatgacggc tttttaccca
    24421 atgtaatcaa cggctctact acacggcgca attccttggc cttaggcaga gttgttacaa
    24481 tagcttcgtg agtcaataat gaattcgcca tattacgcag cattgcagca cgatgactgc
    24541 tggtacggtt taatttgcga ttgccattac gatgacgcat atcaattatc ctttaattct
    24601 tcattaaggc ttttccaagc ctacaggtgg ccatgcttcc aacttagaac ccagtgtcaa
    24661 acctttagat gccaatactt ccttaatctc attcaaagat ttacgtccca aattcggcgt
    24721 tttaagaagc tcggtttcag tgcgttgaat caaatcgcca atataataaa tatcctcagc
    24781 tttcaaacaa ttagctgaac gtactgtcaa ttccagatca tccaccggac gcaaaagaac
    24841 agggtcgata ggaggtgctt tttcttcaac ctcctccaca ggcgtaccct gcaaatcagc
    24901 aaaaatagac atctgatcaa tcaaaatacg tgccgcactg cgtacagctt cctcaggatc
    24961 aatagaaccg tcggtttcga tatccaaaac caacttatca agatccgtcc gctgctctac
    25021 acgtgcaggt tcaacctcaa agctaacacg gctgatgggc gaaaagctcg catccaactg
    25081 gattgcacca atctgacggt tctcatcacg aactacctga cgacctgaaa cagattgata
    25141 accacgacct tgctctactt taatttccat ctcaatttga ccgttatcag ccaaatgaca
    25201 aatgacatga ccagggttca gaatttctac atcatgcggc aactcaatat caccggcaga
    25261 tacgacacct gaacctgatt tcttcaacac aagttgaact tggctacgac catggagttt
    25321 aaacacaata cctttaatat tcagcaaaat gtcaacaaca tcttcctgaa taccatcaac
    25381 agtagaatat tcgtgtaata caccggcaat agctacttca gtaggagcaa aaccattcat
    25441 ggatgacagt aagatacggc gcaaagcatt acctaaggta tgaccgaaac cacgttcaaa
    25501 tggctgcata gatacttttg cacgagttgc agaaaaagta tttacatcaa tttgacgagg
    25561 tttcaaaaat tcggttgtgc tattctgcat ttaactgtcc ctcactgagc tagcattatt
    25621 tagagtagaa ctctaccacc agctgttcat taatatcacc ggtcaattcc gagcgatccg
    25681 gcatgttttt gaacacaccc tcaagtttat ccgcatctac agaaacccaa cccggcaagc
    25741 cgatttgagt tgccaaaccc aatgcttctt gaatacgtac ctgttttttg gctttttcac
    25801 gaactgagac aacatcacca gctttcactt ggaaagaagg aatattgaca acttgtccat
    25861 tcacaactat cgccttatga gaaacaagct gtcttgcttc agctcgggta gaaccgaaac
    25921 ccatacgata aacgacatta tccaaacgag attccagcaa ctgcaacagc aactcgccgg
    25981 tagaaccttt acgacgatca gcttctgcga aataacgacg gaactgacgt tctaatacgc
    26041 cataaatacg gcggattttt tgtttttcac gcaactgcaa accatagtct gacaaacgcg
    26101 gtttttttgc accatgctga ccgggagcgg aatcaatttt acatttagaa tccaaagagc
    26161 ggcgcgcact cttcaaaaac aaatccgtac cttcgcgacg tgccaactta catttagggc
    26221 caatataacg tgccatgttt caaatcactc caatattaaa tacgacgttt tttaggcgga
    26281 cggcaaccgt tatgaggcaa cggggtaacg tcagtaatgc tggtaatctt gaaaccaaga
    26341 gcattcaaag cacgtacaga ggattcacga cctggacctg gacctttaat acgaacctct
    26401 aaatttttaa cgccatactc ttgggcaact ttaccagctg cttctgctgc aacttgtgct
    26461 gcaaatggtg tacttttacg agaaccttta aaaccagcgc cgccagaggt agcccaagac
    26521 aacgcattgc cttgacggtc agtgattgta atgatggtat tgttgaaaga tgcatgaacg
    26581 tgcacaatac cctcactcac ggttttacgt acttttttac gtacacgtga agctgtgttt
    26641 gctttagcca ttaataaaat tccttaaaat ttatttctta ccagcaatcg ctttacgcgg
    26701 acctttgcgg gtacgcgcat ttgtacgagt gcgttgaccg cggcatggta agccgcgacg
    26761 atgacggaag ccacgatagc agcccatgtc catcaatcgc ttgatactca tagttacctc
    26821 acgacgcaaa tcaccttcta cttcatactt ggcaacttgg tcacgcaaag catctaattg
    26881 agtctcgtcc aaatcttttg ctttagtatc aggcgcaata tttgcagcct cacaaatcaa
    26941 tttagcacga gtagcaccaa taccgtaaat agcctgaaga ccaattacga tgtgtgcgtt
    27001 attagggata tttacccctg caatacgagc catatttttt cctttttagg gcaaagtgtg
    27061 tcactatatc acagaatcac attaaaagaa atattccatt aaccttgacg ctgtttgtga
    27121 cggagatcag tacaaattac acgaactaca cgatttcgac gaataatctt gcaatttcgg
    27181 caaattttct taacagatgg ttgtacacgc attttatttt ccttttattg gtttatcttg
    27241 ctctgaaaac gattcgagcc ctagttagat cataaggtgt cagctctact gtgaccttat
    27301 ctcccggaga aatacgaatg taatgcatcc gcatcttccc agaaatatga cccaatacaa
    27361 tatggtcatt ctcaagtttt actttaaatg ttgcattagg taaagtttca agaatttcac
    27421 cttgcatttg gatagtatct tctttcgcca taattctatt tccgagaaaa tgatttcatt
    27481 tctgaacgag tcattaactt atcatactgt tgagtaagcc tatacgaatt tatttgtgta
    27541 ctaaaatcca tcgttacaac aactagaatc aacaaagacg tgccacccaa ataaaaaggt
    27601 acatttaaaa ccgtagttaa gaactctgga attaaacaaa tagttgtaat ataaagagct
    27661 ccaaacaatg tcaaacgtaa tacaactttt tctaaatacc tagaggtctg ctcaccaggt
    27721 ctaatcccag gaacaaaagc accactcttt tttaaattct ctgccatttc tttagggcta
    27781 aaaaccaaag ccgtataaaa ataacaaaag aaaataactg tcgctgcaaa taaagccata
    27841 tacagcaatt gaccgtgttg taacaatcca gctattttgt gcaaaacact atttgtatca
    27901 gccgaaccaa accaacctaa aagagtagat ggaaatagaa taatactgga agcaaaaatt
    27961 gggggaataa caccagccat attcaacttg aaaggcatat gcgtattttg actacccgcc
    28021 ctaccattaa actggcgttt tgcataatga ataggaatct tccgctgtgc actttcaaag
    28081 tatacaacca aataaattaa taataaggca ccaaatacaa taaacaacgc cgtaagcatg
    28141 ctcatagaac cttggttcgt cagtgtaacc agctttgcaa tacccgaagg aatacctgaa
    28201 gcaatacctg ccgtaatgat taaagaaata ccgttcccga tacccctttc agtaatttgc
    28261 tccccaagcc acataagaaa catggttccc gttaccaaag aaactaccgt ggaaacatga
    28321 aactcaaatg aacttgttac aacaattcct tgctgaaata cgaaagatgc aacacctaga
    28381 ctttgaagaa ttgctaacaa aacagtacca tacctagtat atttcgtaat tacctttcta
    28441 ccagcctccc cttctttttt taaagccttc aatgatggca aaatttcaga agcaagctgt
    28501 acaataatag aagctgaaat atatggcata attcctattg caaatatact aaagcgctct
    28561 aacgacccac cggaaaacat attcaatatt cccaggatgc cgtttccagc gctttcgtat
    28621 aatttagcta aagcaacagc atcaactcca ggtacgggta tatgggcacc aattcgaaaa
    28681 acaatcaatg ctccaaatag aaacaaaaga cgtttcttaa gatctccaaa tttggatgaa
    28741 cctgatgacg tttgttgatt agccacaata ttaaacctta catttcaatc ttaccaccaa
    28801 cagcctcgat agcagctctc gcacctttgg taactttaat acccttcaaa gcaactgcct
    28861 tagaaatttc accagaagca ataactttaa cattagagac tgtagatgca atcagacccg
    28921 cttgctttaa gaccaaaata tcaatctcat taacagcaat tgattccagt tcacttaaac
    28981 gaagctgtgc attagctgat gctgttaaag atttaaaacc tcttttaggg aggcgtcgtt
    29041 gcaagggcat ttgaccaccc tcgaaaccca ccttatgaaa cccaccagac cggctctttt
    29101 gacctttatg accacgacca cccgttttgc caagaccact accaataccg cgtccaacac
    29161 gacgaccagc atgcgtagca ccaacagcag gttgaattgt attcaaaaac atatcaagac
    29221 tccactttca acaagtagct gattttatta atcataccac ggttttcagg ggtatctaaa
    29281 acctctaccg tatgctcgcg acgacgcaaa cctaaaccgc gtgcacatgc acgatgagat
    29341 tcaattgtac caatcaggct tttaaccaat gtaaccctaa tctttttttg ttcagccatg
    29401 gttaactccc aaaatgtctt ccactgtcaa gccacgtttg gctgcgatat cagcaggagt
    29461 atgcaactta gacaaaccat ctaatgttgc acgtacgata ttatatgggt tagtagatcc
    29521 gtgcactttg gcggagatat tatgaatgcc catagcatca aaaaccaaac gcataggtcc
    29581 accggctttt acgccactac cctctttagc aggctgcata aatactttag tagcaccatg
    29641 tcgaccaata acctcatgat gaatcgtacc attttttaat ggtaccttaa tcatagagcg
    29701 tcgtgcttga tccatcgcct tttggactgc aacaggcact tcttttgact tacctttacc
    29761 cataccaata cgaccatctc catcaccaac aacagttagc gcagagaaag ccataatgcg
    29821 accaccttta actactttgg ttacacggtt aactgcgacc atcttttcaa tcagaccgtc
    29881 accgcgttct tcaatttcat gttttgccat ctgaaagtct ccaaatattt agaagcttaa
    29941 accattttca cgagcagctt cagccaaagc cttcacacga ccgtgatatt ggaaacctga
    30001 acgatcaaaa gcaacctttt ctacacctgc tgctttagcc ttctcagcaa tacgtttacc
    30061 aactattgca gctgcttcaa cattgcttcc agatttcaga ctaccgcgca cctcagcttc
    30121 caatgtagag gcttgagcca atactttatc accttcagca ctaattactt gagcataaat
    30181 atgattattg cttcggaaca cacataatct taccattttc aagtccgcaa tacgagcacg
    30241 ggttttgcgt gcacgacgga gtcgggttgt atgtttatcc attagtgaac ctcaattatt
    30301 ttttcttggc ttctttcatt accactactt ctcctacata gcgaacacct ttacctttat
    30361 aaggctcagg agcacggaac gcacgaatct cagcagcaac ttgaccaaca acttgtttat
    30421 ccgagccggt taaaacaatc tctgtttggc taggagtttg aacggagaca ccttcaggca
    30481 tttcatatac gatcggatga gaaaaaccca aagacagatt caagatttta ccttgtgctt
    30541 gagcacgata acccacacct atcaattgca atcttttctc aaaaccttct gaaacacctt
    30601 taaccatatt gctgactaat gcgcgagcag taccagacat tgcatttgct tgtttactgc
    30661 tgttattcgc aacaaaagcc aatttgccat cattaaattc aatggctaca tcagaatgca
    30721 aaggaaaaga caattcaccg ttcttaccct taataactaa tgcctctgtt ccaaatttta
    30781 cttctacacc agcgggaaca gtcactgggt tttttgcgac acgtgacatt tcttttcctc
    30841 cactaggcta caatgcataa caactcacca ccaacacctt gagaacgtgc tttacgatca
    30901 gtcattacac ctttagaagt actaacaata gcaataccca agccattcat aacacttgga
    30961 atctcactag acgctttata aatacgcaaa ccagggcgag atacacgctt gatttgttca
    31021 attacaggac gacctgcata gtattttaat tgaatttcca atatagactt tacgtcagat
    31081 gaaactgcga agtcctcaat atatccttct tctttcaata cctttgcaat agcacacttt
    31141 aattttgaag aaggcattgc aaccgctgct ttattagcac gttgcgcatt gcggatacga
    31201 gtcaacatat cggaaatagg atcatgcata ctcattctta attactccta ttaccagctg
    31261 gctttaacaa cacccggaat ttcgccacgc atggcgattt cacggatttt aatacgaccc
    31321 aaaccaaatt tacggaaagt accacgaggg cgacctgtca aagcacaacg acgacgttga
    31381 cgcacaggtg ccgcattacg aggaatggat tgaaacctca aacgagcctc aaaacgctct
    31441 tcctcagttg cattcgaatc attgattacc gctttaattg ccgcgcgttt agccgcatat
    31501 tttttagcca aagcttgacg tttcagatca cgattaataa gtgctttctt agccatgatt
    31561 atcctttgaa cggaaattta aacaatgaca ataaagcttt cgcttcctca tcggtttttg
    31621 ctgtagtagt aatagtaata ttcaaaccac gcaaagcatc aattttatcg tattcaattt
    31681 ccggaaaaat aatttgctca cgaacaccca tattgtaatt gccacggcca tcaaatgatt
    31741 taccgctcac accacggaag tcacgtacgc gaggtaatgc aatagtaatc aaacgatcca
    31801 agaattcaaa catttgatca cgacgcaatg ttactttgca accaaccgga tagttatcac
    31861 ggattttaaa acctgcgata gatttacggg caacagtaac aaccggtttt tgaccggcaa
    31921 ttttctctaa atcggaaaca gcatgttcca taactttttt atcagcaaca gcctcaccca
    31981 cacccatatt caaggtaatt ttttcaatac gcgggacttc cattactgat ttgtaaccaa
    32041 attgtttaac caattcagga acaactgtct ctttataaaa ctctctcaac cgagccatgt
    32101 tatctcctta tgccccaatg atagagccat ttgatttgaa gaaacgaacg cgtttaactt
    32161 tgccttcatt ttcaatcagc ttaataccaa cacggtccgc tttattagtt tccggattca
    32221 ggattgcgat attagaaata tccaaaggca tttctttagt aataataccg ccctcaatgc
    32281 cacgcattgg attaggtttt tgatggcgtt ttacaacatt aacgccctca acaacaactt
    32341 tatcacccaa cactcgaact acttgaccct gcttaccttt atccttacca gcaattacta
    32401 caaccctatc gcctttaatg attttattca tcgtgccatt ccttataata cttcaggtgc
    32461 caatgaaacg attttcataa atcgctcagt acgcaattca cgggttaccg gaccaaagat
    32521 acgagtaccc aaaggttcaa gtttattatt cagtaacacg gcggcattgt tatcgaattt
    32581 aattaacgca ccatcaggac gacgtacacc cttagcagta cgaacaacta ccgcattata
    32641 tacatcgcct tttttgacac ggccacgcgg agccgcatct ttaactgcca ctttaataat
    32701 atcgccaaca gaagcgtagc gacgcttaga tccgcccaat accttgatac acattacgcg
    32761 acgcgcacca gagttatcag ccacatctaa gatggtctgc atttgaatca tgttattacc
    32821 tttaaattaa ccaacttaat ttaccacttt cagataactt acagcaaagc cgctgcttat
    32881 taaatgaaac cttgcggttc aagtaaacca gtcttggacc ccgaaaggga agaaacttcc
    32941 agaagaaact gaaagacagg aaacgaagtt tacacgcaaa ttaaatttgc tgcaatactt
    33001 cgtttcccat tccaagcacg ttgctttaat tcttaaatag aacgtgcttt ctcaaccagc
    33061 tcactgacaa cccaagattt agtttttgac aatggacggg attccgatat aacaaccaca
    33121 tcaccaattc catattgatt attttcatca tgggcatgga ttttagtaga taaccgaata
    33181 atcttaccat aaagcggatg ttttacttta cgctcaacca atactgttac ggttttatcc
    33241 attttgtcgc ttactacttt gccttgcaaa gtacgaacat ttttagtttc gctcattact
    33301 tagcaccttt ttcagttaaa acggttttaa tacgagcaat atcgcgacgt acacgtttca
    33361 attcacttgg tttgcctaat tgaccggtag cgttttgcat acgtaagcca aactgagctt
    33421 tcaacaagtc caacaaatct gcattcaact gctcaacgga tttgtctttc aattcatttg
    33481 ctttcattat tgacccacct gtcttactac aaaggttgta ggaataggca atttggcagc
    33541 agccaactcg aatgcttcac gagccagttc ctctggaacg ccatccattt catacaacac
    33601 tttacctggt ttaatttcgg caatgtaata ttccacgtta cctttaccgc cacccatacg
    33661 aacttgaata ggcttttcag taatcggttt atcagggaat acacgaatcc aaatacgacc
    33721 accacgtttg atatgacggg tcattgcacg acgagcagct tcgatttgac gggcagtcaa
    33781 acgaccacga cctacggctt tcaagccgaa ctcaccgaaa cttaccttat taccgcgagt
    33841 agcgatgccg gtattgcgac ccttttgttg cttacggtat ttcagtctag ttggctgcag
    33901 cattacgtct acctgccttt ctttgtttac tctcatgttc aggtttggaa gatttaatat
    33961 tgccttccgt ataaacccaa actttcagac ccaatacacc atatgtggtg tgcgcttcgc
    34021 tggttgcata atctacattt gcacgtaaag tatgcagtgg cacgcgacct tcacgatacc
    34081 attcgctacg ggcaatatcc gcaccattca gacggcctga agtcataatc ttaatgcctt
    34141 tagcaccaga acgcattgca ttttgcattg ctcgtttcat agcacgacgg aattgaacgc
    34201 gcttttccaa ctgctgggca ataccgtcag caataatttg agcatccaac tcaggacggc
    34261 ggatctcttc aatatttaca tgaacaggta cacccatcaa gacttgcaag tcacgtttca
    34321 aaacctcgat atcctcacct tttttaccga taaccacacc cggacgagcg gagtgaatgg
    34381 taatgcgtgc agattttgca gggcgttcaa taaccactcg accaaccgaa gcattggcca
    34441 atttttgacg caaataattg cgaacatcga tatcctgctt caaaacagta gaaaagtcgg
    34501 tgcttttagc aaaccatttt gaagcccagt ctttagttac cgccaggcga aagcctgtag
    34561 ggttaatctt ttgtcccata gcttttcctt agttacccac tgtcacattg atatgacaag
    34621 tttgtttttc gatgcggtta ccgcgacctt tggcgcgagc ttgaaaacgt ttcaagcttg
    34681 ggcctttgtc aacaaagata gttaccactt tcagttcatc aatgtccgca ccgttattgt
    34741 gctcggcatt agcaatagct gactccaata cttttttaat cagctcggca ccttttttag
    34801 gactgaaagc caaaatattc aaagcttggg caacgtcttt accacgaatc aaatcagcta
    34861 ccaaacgagc cttttgagca gagatacggg catttttatg ttgtgcattt actctcatga
    34921 ttcaccttat ttctttttag cctttttatc ggccaagtgg cctttaaagg tacgggtcaa
    34981 tgagaattcg cctaatttat gaccaaccat attgtcgctg ataaacacag gcacatgggt
    35041 gcggccgttg tgcacagcaa tggtcagacc gataaaatca ggcagaatgg tagaacgacg
    35101 agaccaggtt ttaatcgggc gtttgtcgtt gcttgcgcga gcagcatcta cttttttcag
    35161 caaatgcagg tctacatatg ggcctttttt caatgaacga gccatactaa attaaccttt
    35221 atttgagtaa cggcgacgaa caatcatgtt atccgtgcgt ttgttattac gagtgcggta
    35281 gcctttagca ggagtacccc atgggctgac cggttcgcgg gcctcgcccg tacggccttc
    35341 accaccacca tgcgggtgat cgacaggatt catgacaaca ccgcgtacgg tcggacgaat
    35401 accgcgccaa cggttggcac cggctttacc gatttttttc aggctttgct cttcgttacc
    35461 aacttcaccg atggtcgcac ggcaatttac gttgatttta cggacttcgc cagagcgcag
    35521 gcggacttga gcgtacgcgc cttctttagc cagcaatacc gcagaagcac cggcagaacg
    35581 tgcgatttga gcacctttac cgggtttcat ttcgatacag tggatagtcg taccaacggg
    35641 aatgttgcga atcggcaggg tgttgcctac tttgatggca gcttcagcac cggaaaccaa
    35701 tacggcaccg gcttgaatac cgcgaggagc aatgatgtaa cgacgctcgc cgtctgcata
    35761 gcacaacagt gcgatgaagg cagtacggtt aggatcgtat tcgatacgct ctactttggc
    35821 aggaatgccg tctttgttac gtttaaagtc tacaacacgg taatggtgtt tatgaccgcc
    35881 gcctttgtga cgggtggtga tatgaccatt actgttacga ccagcagtag aatttttctt
    35941 ttcgagcaaa ggtgcataag gcgcaccttt gtgcaaacct tctgttacca cgcgaaccat
    36001 gccgcgacgg cctgcagagg tcggcttcat tttaacgatt gccattttgt ttattcctta
    36061 tctgcagctg cagcagcggc ttccaaatcc aactcttgac cggcagccaa gcttacataa
    36121 gcttttttaa catcgcggcg gcggcccaaa gtacgaccaa aacgcttagt tttgccttta
    36181 gttgtaacgg tagttacaga agcaacttga acaccaaaca gcagctcgac agccgctttg
    36241 atttcaggtt tggttgcact tgccaagact ttaaacgtca tttggttgcg tttttcagcc
    36301 aatacgttgc ttttttcaga aacgataggt gccaaaatca cttgagtcaa acgttgttga
    36361 ttcataccca ttgctcctct aattgtgcaa ctgcatcttt agtgatgatt acttttttgt
    36421 aacgcagcaa gctgtaagga tcaacttgtt gagcttccaa aaccaacacg tttggcaagt
    36481 tgcgtgaagc caagtaaaca ttctcgtcga gctgtttggt tacaaacaac acttgctcca
    36541 gacccagatt tttcacttgt tcagcaaaaa ctttggtttt aggagtttcg gcagtcaacg
    36601 cctcaatcgc aaacaaacgc tcgtcacgag tcaattggga cagaatagtc gccataccgg
    36661 cacggtacat tttgcggttt actttttgag tgaagttttc gtcgggtttg ttcgggaacg
    36721 cgcgaccacc tttacgccac agcggagatg aagtcatacc ggaacgggcg cggccggtac
    36781 ctttttgacg ccatggtttt ttggttgagt gttttacttc ggcacgggtt ttttgagcac
    36841 ggttaccaga gcgggcgttt gccaagtagg catttaccag ctgatgaacc aacgcttcat
    36901 tgtattcgcg ggcgaacaaa gcatcagaaa cagacaggct gcctgaaact tgtcctttag
    36961 cgtcaattac tttcaattcc attacgcacc tactttcacg ctgggacgaa ctacaacatc
    37021 gctgttgacc gcacccggaa cagcaccctt aaccaacagc agttggcgtt ctgcatcaac
    37081 acggacaact tccaattttt gaacagttgc tttggtgttg ccgtattggc cggccatgcg
    37141 tttaccgggg aacacgcgac ccgggtcttg cgccataccg atagaacctg gaacgcggtg
    37201 agaacgggag ttaccgtggg aagtacgttg ggcaccgaag ttatgacgtt taatcgtgcc
    37261 ggagaaacct ttacctttag agataccggt tacatcgacc agttgaccga cttcaaacat
    37321 agaaacggtg atttcgtcac cagctttcaa ttcagccagt ttttcttcag tcaaagcaaa
    37381 ctcaatcaaa ccgcgaccgg cttcaacacc tgcttttgca aagtgcccgg cttcggcttt
    37441 gttgacacga ttggcttttt tctgaccaaa ggtaacttga acggcagtat agccgtcagt
    37501 atctttggat tttacttgtg taacgcggtt ggcagacata tccaaaacgg ttaccggaac
    37561 agaaacaccc tgttcgtcga acacgcgggt cataccaact ttgcgtccaa ccagacctaa
    37621 agtcatgatt attttccttt taaagtaaag ggacaggcta cgattggcct gtctttcaga
    37681 caaagttaaa cttggcacaa atgtaccaag ctcggcatta tatctgacaa gttaaagaaa
    37741 tatcaacaga aatatcagaa atatcttcaa tatgccgtct gaagcgtttt gaattttcag
    37801 acggcatagg gtcggactct tttaaagggt cggtatccgg tcatcacagc gtatgcatct
    37861 ttctccgaca aaccggaata tggttgatga ctgtaatcgg ggatgctcag ttcgtcaaag
    37921 cggcaaaaca ggttgaaatc gatgcgggtg atgaggctgt gctcgagttc gggatcggag
    37981 aggctgtgcc attgtccgag caggacggct ttgaacatgg atagcagggg ataggcggga
    38041 cggccgcggt ggtctctaag gtaacgggtt ttttgacggt tcaggtattg ttcgatcagc
    38101 tgccaatcaa tcacccggtc caacttcaat agcgggaagc ggtcgatgtg tttagcaatc
    38161 atggcttgtg cggtttgttg aaagaaggtg ctcatggaaa atctcctaaa tgtcttggtg
    38221 ggaatttagg ggattttggg gaattttgca aaggtctcgg cataacataa aaaatcccca
    38281 agcattgatt gcttggggat tttcaatagt ccgaattatt ggactttgat ttctacatca
    38341 acaccggccg gcaaatccag cttcatcagc gcatcggtag ttttatcggt ccaatccacg
    38401 atgtccatca ggcgcaagtg ggtgcggatt tccaattgtt cacgggaagt tttgttcacg
    38461 tgcggagaac gcagaatgtt gaaacgttcg attttggtcg gcaaaggaat cgggcctttt
    38521 acaacggcac cggtacgttt tgcagtttca acgatttctt gtgcagaacg gtcaatcagg
    38581 gcgtaatcat aagctttcag gcggatacgg attttttggt ttgccattta tcaatatcct
    38641 tcaattaagc gataacagaa gaaaccacgc cggcacccac ggtacggccg ccttcgcgaa
    38701 tcgcaaagcg caggccttct tccatagcga taggcgcaat cagttctacg gtgatggtta
    38761 cgttttcacc cggcattacc atttccacac cttcttccaa agtaaccgcg ccggttacgt
    38821 cggtggtacg gaagtagaat tgcggacggt agttggcgaa gaatggggta tg
//
LOCUS       DAWFJW010000006        38671 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFJW010000006 DAWFJW010000000
VERSION     DAWFJW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.006"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            232..510
                     /locus_tag="WGR19_000243"
     CDS             232..510
                     /locus_tag="WGR19_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797845.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            529..1677
                     /gene="earP"
                     /locus_tag="WGR19_000244"
     CDS             529..1677
                     /gene="earP"
                     /locus_tag="WGR19_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0797846.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSLRLARVLHRELGWRVHLWTD
                     DVSALRALCPDLPDVPCVHQDIDVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFEQPSAPEKLAAFVSKHQKIR"
     gene            1720..2280
                     /gene="efp"
                     /locus_tag="WGR19_000245"
     CDS             1720..2280
                     /gene="efp"
                     /locus_tag="WGR19_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0797847.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(2330..3166)
                     /locus_tag="WGR19_000246"
     CDS             complement(2330..3166)
                     /locus_tag="WGR19_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0797848.1"
                     /translation="MKNTTSSLLLWLAAIMLAACSPSKNDKTKEVGASAASSSASSAS
                     SQTDLQPAASAPDNVKQAESAPPSNCTGLHPASGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            3260..3366
                     /locus_tag="WGR19_000247"
                     /pseudo
     CDS             3260..3366
                     /locus_tag="WGR19_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3388..3969)
                     /gene="metW"
                     /locus_tag="WGR19_000248"
     CDS             complement(3388..3969)
                     /gene="metW"
                     /locus_tag="WGR19_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0797849.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(3966..5105)
                     /locus_tag="WGR19_000249"
     CDS             complement(3966..5105)
                     /locus_tag="WGR19_000249"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0797850.1"
                     /translation="MSQNASVGIVTPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGKHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIEQWAAVIGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            5265..5465
                     /locus_tag="WGR19_000250"
     CDS             5265..5465
                     /locus_tag="WGR19_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797851.1"
                     /translation="MIGRLFRIFFFFALAALIIKRLFSRRQKRALCEVAKISAWVLLG
                     SAAATLFWYLVMLYFKHIPDSY"
     gene            complement(5726..5851)
                     /gene="ykgO"
                     /locus_tag="WGR19_000251"
     CDS             complement(5726..5851)
                     /gene="ykgO"
                     /locus_tag="WGR19_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0797852.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(5851..6126)
                     /locus_tag="WGR19_000252"
     CDS             complement(5851..6126)
                     /locus_tag="WGR19_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0797853.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            6327..7205
                     /gene="metF"
                     /locus_tag="WGR19_000253"
     CDS             6327..7205
                     /gene="metF"
                     /locus_tag="WGR19_000253"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0797854.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            7343..9619
                     /gene="metE"
                     /locus_tag="WGR19_000254"
     CDS             7343..9619
                     /gene="metE"
                     /locus_tag="WGR19_000254"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0797855.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTIVGPL
                     TFLWVGKEKGVVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLKTVESLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAAGSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSVADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            9898..10635
                     /locus_tag="WGR19_000255"
     CDS             9898..10635
                     /locus_tag="WGR19_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0797856.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWSAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            10884..12287
                     /locus_tag="WGR19_000256"
     CDS             10884..12287
                     /locus_tag="WGR19_000256"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0797857.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            12566..12943
                     /gene="sdhC"
                     /locus_tag="WGR19_000257"
     CDS             12566..12943
                     /gene="sdhC"
                     /locus_tag="WGR19_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0797858.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            12937..13278
                     /gene="sdhD"
                     /locus_tag="WGR19_000258"
     CDS             12937..13278
                     /gene="sdhD"
                     /locus_tag="WGR19_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0797859.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            13281..15044
                     /gene="sdhA"
                     /locus_tag="WGR19_000259"
     CDS             13281..15044
                     /gene="sdhA"
                     /locus_tag="WGR19_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0797860.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDINTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            15164..15871
                     /locus_tag="WGR19_000260"
     CDS             15164..15871
                     /locus_tag="WGR19_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0797861.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            15875..16123
                     /locus_tag="WGR19_000261"
     CDS             15875..16123
                     /locus_tag="WGR19_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0797862.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            16235..17518
                     /gene="gltA"
                     /locus_tag="WGR19_000262"
     CDS             16235..17518
                     /gene="gltA"
                     /locus_tag="WGR19_000262"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0797863.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            17723..20551
                     /locus_tag="WGR19_000263"
     CDS             17723..20551
                     /locus_tag="WGR19_000263"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0797864.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            20651..21844
                     /gene="odhB"
                     /locus_tag="WGR19_000264"
     CDS             20651..21844
                     /gene="odhB"
                     /locus_tag="WGR19_000264"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0797865.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     APAAAQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVT
                     LPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEK
                     EHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPIL
                     RDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQ
                     SAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPAR
                     LLLDL"
     gene            complement(21896..22012)
                     /locus_tag="WGR19_000265"
                     /pseudo
     CDS             complement(21896..22012)
                     /locus_tag="WGR19_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            22209..23642
                     /gene="lpdA"
                     /locus_tag="WGR19_000266"
     CDS             22209..23642
                     /gene="lpdA"
                     /locus_tag="WGR19_000266"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0797866.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDHEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            23767..24054
                     /locus_tag="WGR19_000267"
     CDS             23767..24054
                     /locus_tag="WGR19_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797867.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            24154..25320
                     /gene="sucC"
                     /locus_tag="WGR19_000268"
     CDS             24154..25320
                     /gene="sucC"
                     /locus_tag="WGR19_000268"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0797868.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            25331..26221
                     /gene="sucD"
                     /locus_tag="WGR19_000269"
     CDS             25331..26221
                     /gene="sucD"
                     /locus_tag="WGR19_000269"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0797869.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(26456..26785)
                     /locus_tag="WGR19_000270"
     CDS             complement(26456..26785)
                     /locus_tag="WGR19_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0797870.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTPPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            27034..27300
                     /locus_tag="WGR19_000271"
     CDS             27034..27300
                     /locus_tag="WGR19_000271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797871.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            complement(27527..28468)
                     /locus_tag="WGR19_000272"
     CDS             complement(27527..28468)
                     /locus_tag="WGR19_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEZ0797872.1"
                     /translation="MEYNAIMKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKIN
                     RKDLMQAFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIK
                     ILNGSLIFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGL
                     SEKVIVPLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDI
                     DWNRIVKIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDH
                     HIDDPAFRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            28606..28875
                     /locus_tag="WGR19_000273"
     CDS             28606..28875
                     /locus_tag="WGR19_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797873.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            28879..29076
                     /locus_tag="WGR19_000274"
     CDS             28879..29076
                     /locus_tag="WGR19_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797874.1"
                     /translation="MEMIMTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKA
                     PPTQNPGSSWIQIKRTPDSSR"
     gene            29091..29261
                     /locus_tag="WGR19_000275"
     CDS             29091..29261
                     /locus_tag="WGR19_000275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797875.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(29293..32139)
                     /gene="uvrA"
                     /locus_tag="WGR19_000276"
     CDS             complement(29293..32139)
                     /gene="uvrA"
                     /locus_tag="WGR19_000276"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0797876.1"
                     /translation="MCKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVCVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            32294..33079
                     /locus_tag="WGR19_000277"
     CDS             32294..33079
                     /locus_tag="WGR19_000277"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0797877.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QTESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            complement(33902..36178)
                     /locus_tag="WGR19_000278"
     CDS             complement(33902..36178)
                     /locus_tag="WGR19_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0797878.1"
                     /translation="MVQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSRADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            36600..37190
                     /locus_tag="WGR19_000279"
     CDS             36600..37190
                     /locus_tag="WGR19_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0797879.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTADGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            37254..38312
                     /gene="trpD"
                     /locus_tag="WGR19_000280"
     CDS             37254..38312
                     /gene="trpD"
                     /locus_tag="WGR19_000280"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0797880.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(38464..>38671)
                     /locus_tag="WGR19_000281"
     CDS             complement(38464..>38671)
                     /locus_tag="WGR19_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0797881.1"
                     /translation="TVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFSH
                     LRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         9628 a         9351 c        10018 g         9674 t
ORIGIN      
        1 tcaaaccgac gcgccgggtt ttcaatatgc ccgtgcctgc tgccgccttg tcctcaggca
       61 tcggcggcag cgtccgattt ttcggggaac gcccgtctcg ggcgtattta aaggttcggc
      121 ggtgcggtgt tttcctgcgg caaggcttca gacggcatct ccggtgcgtc cgttagacaa
      181 ggcgtgcgct tggggcgata atggcgtttt gctttttttg aaagccttac aatgtctcga
      241 aacctgcttg tccgttggct tgccgtctgc ctcatcccgt tggcgacgct tgccgttttc
      301 gccgccaatc cgcccgaaga caaactccag catctgatca acggcatcat ccttgcctgc
      361 gaagcgacgt ttttgttcaa attcgtcctt ttcgacacca tcaagcatca tttgaagcaa
      421 gagtttgatt tgaaacgtca aactatgttt ctgtttattc cgattgtttt gctggttgtg
      481 tatctgttcc actatttcgg cgcgttttag cccgtttccg ttattcctat gaatactcct
      541 ccttttgtct gctggatttt ttgcaaggtc atcgacaatt tcggcgatat cggcgtttcc
      601 ctgcggcttg cccgtgtttt gcatcgcgaa ctcggttggc gggtgcattt gtggacggac
      661 gatgtgtccg ccttgcgtgc gctttgccct gatttgcccg atgttccctg tgttcatcag
      721 gatattgatg tccgcacttg gcattccgat gcggcagaca ttgataccgc gcctgttccc
      781 gatgtcgtca tcgaaacttt tgcctgcgac ctgcccgaaa atgtgctgca cattatccgc
      841 cgacacaagc cgctttggct gaattgggaa tatttgagcg cggaggaaag caatgaaagg
      901 ctgcacctga tgccttcgcc gcaggaaggt gttcaaaaat atttttggtt tatgggcttc
      961 agcgaaaaaa gcggcggact gatacgcgaa cgcgattact gcgaagccgt ccgtttcgat
     1021 agcggagcct tgcgcaagag gctgatgctt cccgaaaaaa acgcccccga atggctgctt
     1081 ttcggctatc ggagcgatgt ttgggcaaag tggctggaaa tgtggcgaca ggcaggcagc
     1141 ccgatgacac tgttgctggc ggggacgcaa atcatcgaca gcctcaaaca aagcggcgtt
     1201 attccgcaag atgccctgca aaacgacggc gatgtttttc agacggcatc cgtccgcctc
     1261 gtcaaaatcc ctttcgtgcc gcaacaggac ttcgacaaac tgctgcacct cgccgactgc
     1321 gccgtgatac gcggcgaaga cagtttcgtg cgcgcccagc ttgccggcaa acccttcttt
     1381 tggcacatct acccgcaaga cgagaatgtc catctcgata aactccacgc cttttgggat
     1441 aaggcgcatg gattttactc gcccgaaacc gcatcggtac accgccgcct ttcagacgac
     1501 ctcaacggcg gagaggcttt atccgccacg caacgcctcg aatgttggca aaccctgcaa
     1561 caacatcaaa acggctggcg gcaaggcgcg gaggattgga gccgttatct tttcgagcag
     1621 ccgtcagctc ctgaaaaact cgctgccttt gtttcaaagc atcaaaaaat acgctagaat
     1681 agcgcgtttt acgacaaccg atttgattgg aaaatcacaa tgaaaacagc acaagaactg
     1741 cgcgccggca atgtatttat ggtcggcaac gatcctatgg tcgttcaaaa aaccgaatac
     1801 atcaaaggcg gccgctcttc cgccaaagtc agcatgaaac tgaaaaacct gctgaccggc
     1861 gcggcttccg aaaccattta caaagccgac gacaaattcg acgtggtcat cctgtcccgc
     1921 aaaaactgta cgtacagcta ctttgccgat ccgatgtacg tctttatgga tgaagaattc
     1981 aaccaatacg aaatcgaagc cgacaacatc ggcgacgcgt tgaaattcat cgttgacggt
     2041 atggaagacc aatgcgaagt taccttctac gaaggcaacc ccatctccgt agaactgccc
     2101 accatcatcg tacgcgaagt cgagtatacc gagcctgccg tcaaaggcga tacttcaggc
     2161 aaagtgatga aaaccgcgcg cctggtcggg ggcaccgaaa tccaagtgat gtcttacatc
     2221 gaaaacggcg acaaaatcga aatcgatacc cgcaccggcg aattccgcaa acgcgcttga
     2281 tttgttgcat tgaaaaatgc cgtctgaaaa cgtttcagac ggcatttttt tatcttcgcc
     2341 ccaagtttgg attgaagtag atgttttttt cgtaaacgac aatacgcgtg attttgccat
     2401 tttcgtcaaa atggatgtct aaaaacggtt tgtcgggatt gcgttcacgg ttggacagcc
     2461 ggaagagtga ttggttttca agcatttctc cgtgtatttt cagatagccg ggcaattggc
     2521 cgatgtattg gaaattgccg ccaagtccgt tgttgtgccg gctcgaatag gcaggggttg
     2581 ccgaaacttc aaaataacgc gtgcgtttcg gctcctcgcc ttgtccgcgg gtttcttgac
     2641 tgccctgtat cagcaacagc gtcgcttctc ggaggcggcg ttcctgttcc atcaaggcat
     2701 cttgtgcctg ccggctgatc gtgcgctttc cgtaacgcaa gtcttcaatt gccagtatga
     2761 ttttctgatg gtagtcggga tcttcagggc ggatgtcggg aaacagcagc cgctgtatgt
     2821 tgtcatagcc tccgccgggt aagccgaaac gggtttccag ttcgtgatgg gaaagggcaa
     2881 acagacagtc cgagtcgatg tgttcggcga tttgctgcat gagatcgtca atgccggagg
     2941 cggggtgcag gccggtgcaa tttgacggcg gcgcgctttc tgcctgcttg acgttatcag
     3001 gggcggatgc ggccggttgc aaatcggttt gggaagaagc tgatgacgcg gaggacgaag
     3061 cagcggatgc accgacttct ttggttttat cgtttttgct cggagaacac gcggccagca
     3121 tgattgcggc aagccataag agaagtgatg acgttgtgtt tttcattcta ttgtttccaa
     3181 tattaaagag gccgtctgaa aacctaccgt ttcatttttc agacggcttc ttgttagtac
     3241 aacggaagaa cctcgttatt atagtggatt aaatttaaac taggacaagg cggcgagccg
     3301 cagacagtac aaatagtacg gcaaggcgag gcaacgccgt actggtttaa atttaatcca
     3361 ctataccaac aaggttctca acctgtctta cccgacgcgg taaaacgcaa ggctgcccaa
     3421 aaggttgggg aaatgtttga cctgtctgtt gcccgtcatc acggcgcgct cgaggacgcg
     3481 tatgttgttt ttggcgcaca acaaatcaaa gtctttgagc gtacaccaat ggatgttcgg
     3541 cgtatcatac caatggtagg gcatacgttc ggaaaccggc atatgtccgc cgagtgcgat
     3601 ttggacgcgg ttgcgccagt agccgaaatt cgggaagctg acaatcgcct gtttggcaac
     3661 gcgcatcagg cagcgcagga ttttttcggt attctgcatc gcttggatgg tttggctcaa
     3721 cacaatcaca tcaaaacttt gatcgttgaa tgcggttaaa ccttcttcca aatcggcttg
     3781 gataacattc acgccgcgcg acatcgcggc aatcacgctg tcggtgtcga tttcgatgcc
     3841 gtagccgctg cattttttgt gttcgaccaa tgcggcaagc agttcgccgt cgccgcagcc
     3901 caagtccaag acgcggctgc cttcgggtat ccggtcgtaa atcagttgca aatcatcgcg
     3961 caggttcatt gctgacatcc cttataaacg ttgttcatat aggcggcgac cgcacgcata
     4021 taggcttcgt cttccattaa aaaggcatcg tgcccgtgtg cggatttgac ttcgatatac
     4081 tgcacggatt tttgggcggc aatcagtgcc ttgaccagtt cgtgcgaacg ttcgggcgcg
     4141 aaacgccagt cggtgctgaa gctggcgaca aagaattttg cctgaacatc ctgcacggcg
     4201 cgggtcaggc tgttgccgaa atccgccgcc ggatcgaaat agtccaaagc cttggtcatc
     4261 agcaaatagg tgttggcatc aaaccgcccg acgaatttgt cgccttgata gcgcagatag
     4321 gattctactt caaattcaac gccatagccg tattgatagc cgttggaacg caaatcgcgt
     4381 ccgaattttt tgcccaaacc gtcttcggca agataggtaa tgtgccccat catgcgggca
     4441 atccgcaagc cccgagcggg aacggtgttg cggctgcggt aatgtccttc gttgaaatcg
     4501 ggatcggtca aaatcgcctg acgcgccaca tcgttgaacg cgatattttg cgtggaaagc
     4561 ttcggcgcgg acgcaatcac caaggcgtgg cgcacgcgtt cgggatagga aatcgtccac
     4621 tgcaaagcct gcatgccgcc caagctgccg ccgataaccg ccgcccattg ttcgatgccg
     4681 agataatcgg caagcgcggc ttgtgatttt acccagtcct tcaccgtaac caccggaaaa
     4741 tccgcgccgt attccctgcc cgtttcagga ttaatcgaca aaggcccgct gctgccgtcg
     4801 cagccgccca gattattcaa accgaccacg aaaaaacgtt ccgtatcaat cggttttccg
     4861 ggaccgacca tattgtccca ccagcccgta tatttatcct ccgccgaatg tttgcccgca
     4921 acatgatggt tgcccgacag cgcgtggcag attaaaaccg cattgttttt ttcagcattc
     4981 agctcgccgt aggtttcaat catcagatcg aaacgcggca aagttttacc gttttccaaa
     5041 accagtggca cctcaaacgg aattttttgg ggcgttacaa tgcccaccga ggcattttga
     5101 ctcatatcct gttccaacaa atgcggcgaa aagcgttatt atatcgcaaa cggcatgatt
     5161 ttttgacacg gtcggacaag cagcctgaca cgccgaaaca ttcgttccca tgccgccgca
     5221 accgaaccat actttttcag acggtcctaa cccagcttcc cgacatgata ggcagacttt
     5281 tccgtatttt tttctttttc gcacttgccg cgttgattat caagcgcctt ttcagccgca
     5341 ggcaaaaacg cgccctgtgc gaagtcgcca aaatcagcgc atgggtactg cttggctcag
     5401 ccgccgcgac actgttttgg tatttggtca tgctgtattt caaacacatt ccggattcgt
     5461 attgacggaa aaaatgccgc ctgaaacgca tttttctgtt tcagacggca tatttgatga
     5521 aaagggcttg cggtaggagg tgctttatag tggattaaca aaaaccagta cggcgttgcc
     5581 tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg gatcggttcc
     5641 gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
     5701 caaccgaagc aggaagggca gggggtcagc gttggcgcgc tttaaaacgc ggattgcttt
     5761 tgcagatgac gtaaactttg cctttacgtc tgacaatctg acagtcgcgg tggcgttgtt
     5821 tggcggtttt gagtgaagac agaacctgca ttatttgtcc tttctaaacg atgacatgac
     5881 ggattggaaa cgttggttga atttgctggc acggccttcg gtgttgacgt tgcgctgttt
     5941 gccagtatag acgggatggg atgcggaaga agtatccagc gaaaacagcg gatattcttt
     6001 gccgtctgtc caaaccatcg tttttccgtg tgtttcggca caggagcgga ttaaccagcc
     6061 ttcattggcg ctgctgtcga aaaaaaggac ggttcgataa ttgtcaggat gaatattcgg
     6121 tttcatatat cgccttagtt gaattgttat aacataacaa actttatcat aaattagaag
     6181 gcttgtacaa ggaaatttaa ctatttttgt aatatattag aaattttcat gataaatctg
     6241 aaaattttga aattgactca tgtttggcgt aactttatta tgttgcctga aaatatcata
     6301 taaaagataa taaaaggtac gcagccatga attatgcaaa agaaatcaat gcgttaaata
     6361 acagtctttc tgatttgaaa ggcgacatta acgtttcgtt tgaatttttc ccgccgaaaa
     6421 acgaacaaat ggaaacgatg ctgtgggact ccatccaccg tctgcaaacc ctgcatccca
     6481 agttcgtatc cgtaacttac ggcgcaaatt ccggcgaacg cgaccgcacg cacggcatcg
     6541 tcaaacgcat caaacaggaa accggcttgg aagccgcgcc tcacctgacc ggtatcgacg
     6601 cttctcccga cgaattgcgc caaattgcca aagattattg ggacagcggc atccgccgca
     6661 ttgtcgccct gcgcggagac gagccggccg gttatgagaa aaaaccgttt tacgccgaag
     6721 acttggtcaa actgttgcgc tccgtcgccg acttcgacat ttctgtagcg gcatatcccg
     6781 aagtgcatcc cgaagcgaaa tccgcacaag ccgacctgat taatttgaaa cgcaaaatcg
     6841 atgcgggcgc gaaccacgtc atcacccaat tcttcttcga tgtggaacgc tacctgcgct
     6901 tccgcgaccg ctgcgtgatg ttgggcatcg atgtggaaat cgtccccggc atcctgcccg
     6961 ttaccaactt caagcagctc ggcaaaatgg cgcaggtaac caacgtcaaa atcccaagct
     7021 ggctgtcgca aatgtatgaa ggtttggacg acgaccaagg cacgcgcaat ctggtggcgg
     7081 caagtatcgc catcgatatg gtcaaagtcc tgtcccgcga aggcgtgaaa gatttccact
     7141 tctatacgct taaccgcagc gagctgactt acgccatttg ccatatttta ggcgtgcgcc
     7201 cttaaagccg tatcaaacag tttcagacgg catctaaggt gtctaaaaag caaaacaccg
     7261 ccccatccga gccattctga tttacaatac cggccgattc ggatcgaacc ggtccttaca
     7321 aaatccaact ggagagttca acatgacaac attacatttc tcaggcttcc cgcgtgtcgg
     7381 cgccttccgc gaattgaaat tcgcacaaga aaaatactgg cgcaaagaaa tcagcgagca
     7441 agaattgctg gctgttgcta aagacttgcg cgagaaaaac tggaaacacc aagctgctgc
     7501 caacgccgat tacgttgccg caggcgattt cactttctac gaccacatcc tcgacctgca
     7561 agtcgccacc ggcgcgattc ccgcccgctt cggcttcgac agccaaaacc tgtctttgga
     7621 acaattcttc caactggcgc gcggtaacaa agaccaattc gctatcgaaa tgaccaaatg
     7681 gttcgacacc aactaccact acttggtgcc tgaattccac gccgataccg aattcaaagc
     7741 caatgccaaa cactacgtcc aacaattgca agaagcccaa gccctcggtc tgaaagccaa
     7801 accgaccatc gtcggtccgt tgactttcct gtgggtgggt aaagaaaaag gcgtcgtcga
     7861 attcgaccgt ctgagcctgt tgcctaaact gttgcctgtt tacgttgaaa tcctgactgc
     7921 tttggttgaa gccggtgccg agtggattca aatcgacgag cctgctttgg ctgtcgattt
     7981 gcctaaagaa tgggtggaag cctacaaaga cgtttacgct actttgaaca aagtaagtgc
     8041 caaaatcctg ttgagcactt acttcggttc tgttgccgaa cacgccgcat tgctgaaagc
     8101 cctgcctgtt gacggtctgc acatcgactt ggtacgcgct cccgagcaac tggacgcgtt
     8161 cgccgactac gacaaagtcc tgtccgccgg cgtgattgac ggccgcaata tttggcgcgc
     8221 caacctgaac aaagttttga aaactgttga gtctctgcaa gccaaactgg gcgaccgttt
     8281 gtggatctcc agctcttgct ctctgctgca cactccattt gacttgtcag ttgaagaaaa
     8341 actgaaagcc aacaaacctg acctgtactc ttggttggca ttcaccctgc aaaaaaccca
     8401 agaattgcgc gttctgaaag ctgcattgaa cgaaggccgt gattctgttg ccgaagaact
     8461 cgccgccagc caagctgccg ccggctcacg cgccaacagc agcgaaatcc accgtgcaga
     8521 cgttgccaaa cgcctggctg atttgcctgc caacgcagac caacgcaaat ctccatttgc
     8581 cgaccgtatc aaagcgcaac aagcatggtt gaacctgcct ctgctgccga cgaccaacat
     8641 cggttctttc ccgcaaacta ccgaaatccg ccaagcacgt gcagctttca aaaaaggcga
     8701 actgtctgtc gccgattacg aagctgcgat gaaaaaagaa atcgccttgg tggttgaaga
     8761 gcaagaaaaa ctggatttgg acgtactggt acacggcgaa gccgagcgta acgacatggt
     8821 cgaatacttc ggcgaattgt tgagcggttt tgcattcacc caatacggct gggtacaaag
     8881 ctacggctca cgttgtgtta aaccaccgat tatcttcggt gacgtaagcc gtcctgaagc
     8941 catgactgtg gcttggtcta cttacgcaca aagcctgacc aaacgcccga tgaaaggtat
     9001 gttgaccggc cctgtaacca ttctgcaatg gtctttcgtc cgcaacgaca ttccccgcgc
     9061 taccgtgtgc aaacaaatcg cactggctct gaacgacgaa gtattggatc tggaaaaagc
     9121 aggcatcaaa gtcatccaaa ttgacgaacc tgccatccgc gaaggcttgc cgctgaaacg
     9181 cgccgattgg gatgcctacc tgaactgggc gggcgaatcc ttccgcctgt cctctgccgg
     9241 ttgcgaagac agcacacaaa tccacactca tatgtgctac tccgagttca acgatatcct
     9301 gcctgcgatt gctgcaatgg atgcggacgt gatcaccatc gagacttcac gttccgacat
     9361 ggaactcttg accgcgttcg gcgaattcaa atacccgaac gacatcggcc cgggggttta
     9421 cgacatccac agcccgcgcg taccgacaga agccgaagtg gagcacctgt tgcgcaaagc
     9481 catcgaggtt gtaccggttg aacgtctgtg ggttaacccg gactgcggcc tgaaaacacg
     9541 cggctggaaa gaaactctgg aacaactcca agtgatgatg aacgtaaccc acaaattgcg
     9601 tgccgaattg gcgaaataag ccgagaccgt atgaataaat accgtctgaa agcctttcag
     9661 acggtatttt gtcctgattt gcggcgcaag ggcgcagttg ccggaaaatc ttttcattgc
     9721 agcttgtttt tttctaattc ggctttatat gtgggaaaca ggcaaatcgg agttgtgttt
     9781 gatagtttta aataatttat attatttgaa ctataaatta tacaaatcat tttgcatggg
     9841 gtagaatgcc cagcgattca cagttatttc tcaaaccaat ctattaagga gcttaaaatg
     9901 gctttgcaag atcgtaccgg tcaaaaagta ccttccgtag tattccgcac ccgcgtcggc
     9961 gacacttgga aagatgtgtc taccgatgat ttgttcaaag gcaaaaaagt agtcgtattc
    10021 tccctgcctg gtgcatttac cccgacttgt tcttcttcac acctgccgcg ttacaacgaa
    10081 ttgttcggcg cgttcaaaga aaacggcgtt gacgcaatct gctgcgtatc tgtaaacgat
    10141 acgttcgtaa tgaacgcttg gtctgccgaa gaagaatccg acaacatcta catgattcct
    10201 gacggcaacg gcgaatttac cgaaggtatg ggtatgctgg tcggtaaaga agacttgggc
    10261 ttcggtaaac gctcttggcg ttactccatg ctggttaacg acggcgtggt tgaaaaaatg
    10321 ttcatcgagc ctgaagaacc aggcgatccg ttcaaagtat ccgatgcaga tactatgctg
    10381 aaattcgttg ctcctgattg gaaggctcaa gaatctgtgg caattttcac taaaccaggt
    10441 tgccaattct gtgctaaagc caaacaagct ttgcaagaca aaggtttgtc ttacgaagaa
    10501 atcgtattgg gcaaagatgc aaccgttact tctgtacgcg ccattactgg caagatgact
    10561 gctcctcaag tcttcatcgg cggtaaatac atcggcggca gcgaagattt ggaagcttac
    10621 ttggctaaaa actgatagct gtttgcttaa ggcggtttga ttaaactgtc tgatataccg
    10681 gatagagtta ttcgggcggt tctacactgc cgctccgaat aactctatat ttataagaga
    10741 atttggatat tgttgcactc aatcgaaatt ttgtttttat ttatctgaat gatgtttttg
    10801 attgggaaaa tatttaaatg ccgtctgaaa ccgatatgtt ctgtgtcggc aatgtttcag
    10861 acgaaaacgg aaggacaaag attatgaaaa aaattcaagc ggatgtcgtc gtaatcggcg
    10921 gcggtactgc cggtatgggt gcgtttcgca atgcccgttt acattcggat aatgtttacc
    10981 tgattgaaaa caatgtgttc ggcacgacct gcgcgcgcgt gggctgtatg ccttccaaac
    11041 tcttgattgc cgccgcagag gcgcgtcatc acgcattgca taccgacccg ttcggcgtgc
    11101 atttggacaa agacagcatc gtcgtcaacg gtgaagaggt catgcagcgc gttaaatccg
    11161 agcgtgaccg ttttgtcggc tttgtcgttg ccgatgtgga agagtggcct gccgacaagc
    11221 gcatcatggg ttcggctaaa tttatcgacg agcataccgt ccaaatcgac gagcatactc
    11281 aaattacggc aaaaagtttc gtgattgcta ccggttcgcg tcccgtcatc ctgccgcaat
    11341 ggcagtcttt gggcaatcgt ttgattatca acgatgacgt tttctcatgg gatacgctgc
    11401 ctaagcgcgt tgccgtgttc gggccgggtg ttatcggttt ggaactgggt caggcattgc
    11461 accgtttggg cgtgaaagtt gaaattttcg gtttgggcgg aatcatcggc ggcatttccg
    11521 accccgtcgt ttcagacgag gcgaaagccg tgttcggcga agaattgaaa ctgcatctgg
    11581 atgctaaaac cgaggtcaaa ctcgacgcag acggcaatgt agaagtccat tgggagcagg
    11641 atggcgaaaa aggcgtattt gttgccgaat atatgctggc agccgtgggc cgccgtccga
    11701 acgttgacaa tatcggtttg gaaaatatca atatcgaaaa agatgcgcgc ggcgtacctg
    11761 ttgccgaccc gctgaccatg cagaccagta ttccgcatat cttcatcgca ggcgatgcgt
    11821 ccaaccaact gcctctgctg catgaagctg ccgaccaagg caagattgcc ggcgataacg
    11881 cgggccgcta cccgaatatc ggcggcggtt tgcggcgcag caccatcggc gtggtgttta
    11941 ccagtccgca aatcggcttt gtcggtctga aatacgcgca ggttgccgcg caataccaag
    12001 ccgacgaatt tgtcatcggc gaagtatcgt tcaaaaacca aggccgcagc cgcgtgatgc
    12061 tggtaaacaa aggccatatg cgcctgtatg ccgaaaaagc caccggccgc tttatcggcg
    12121 cggaaatcgt aggccctgcc gccgaacatt tggcgcactt gttggcatgg gcgcatcaaa
    12181 tgaagatgac cgttccgcaa atgctggata tgccgttcta ccatcccgtt atcgaggaag
    12241 gtctgcgtac cgcgttgcgc gatgccgatg cgaaattgaa agcctgaccg atatggcaaa
    12301 acaatgccgt ctgaaatttt ttcagacggc attttgtttt tggggatggg gtcggatgct
    12361 gataccgtgt cgggaagggg gcggcaaaac taaaaatctt tcttttaatc tgctgtttcc
    12421 acgcgtgttt gtcaaaatct atcagtttgt ttttaaaata cactgttcaa aatgggataa
    12481 aacaggtaaa ttaacgttta tgtaacccag tgtagcaatg ggtttacggt ttttgagtcg
    12541 atatataact acagaggaat tgactatgtc tgccaaaccg cgtcctgttt atctggattt
    12601 gccgaacatc cgtctgccga tacccgggat agtttccatc cttcaccgca tcagcggggt
    12661 cgggctgttt attatgctgc ctttcctgct gtatttcctg tccggtaccc tgagtcaaga
    12721 gtctgcattt gaaacttacc gtgccattgt ttcccatcct ttggtcaagc tggttttaat
    12781 cggtgtattg tgggcttatc tgcaccattc tctcgccggt atccgctttt tatttttgga
    12841 tgcgcacaaa ggccttgagc tgaatactgc gcgcaatacc gctaaagccg tatttgcttc
    12901 tgcattggtt ttgactgtcg ttttgggagc gttgttatgg tagaacgtaa attgaccggt
    12961 gcccattacg gtttgcgcga ttgggcgatg caacgtgcga ctgcggttat tatgttgatt
    13021 tataccgttg cacttttagt ggttctattt tccctgccta aagaatattc ggcatggcag
    13081 gcatttttta gtcaaacttg ggtaaaagta tttacccaag tgagcttcat cgccgtattc
    13141 ttgcacgctt gggtgggtat ccgcgatttg tggatggact atatcaaacc cttcggcgtg
    13201 cgtttgtttt tgcaggttgc caccatcgtc tggctggtcg gctgcttggt gtattcaatt
    13261 aaagtaattt gggggtaagt atgggttttc ctgttcgcaa gtttgatgcc gtgattgtcg
    13321 gcggtggtgg tgcaggttta cgcgcagccc tccaattatc caaatccggt ctgaattgtg
    13381 ccgttttgtc taaagtgttc ccgacccgtt cgcataccgt agcggcgcag ggcggtattt
    13441 ccgcctctct gggtaatgtg caggaagacc gttgggactg gcacatgtac gataccgtga
    13501 aaggttccga ctggttgggc gaccaagatg cgattgagtt tatgtgccgc gccgcgcctg
    13561 aagccgtaat tgagttggaa cacatgggta tgccttttga ccgtgtggaa agcggtaaaa
    13621 tttatcagcg tcctttcggc ggccatactg ccgaacacgg taaacgcgcg gtagaacgcg
    13681 cctgtgcggt tgccgaccgt acaggtcatg cgatgctgca tactttgtac caacaaaacg
    13741 tccgtgccaa tacgcaattc tttgtggaat ggacggcaca agatttgatt cgtgatgaaa
    13801 acggcgatgt cgtcggcgta accgccatgg aaatggaaac cggcgaagtt tatattttcc
    13861 acgctaaagc tgtgatgttt gctaccggcg gtggcggtcg tatttatgct tcttctacca
    13921 atgcctatat gaataccggc gatggtttgg gcatttgcgc gcgtgcgggt attccgttgg
    13981 aagatatgga attctggcaa ttccacccga ccggcgtggc aggtgcgggc gtgttgatta
    14041 ccgaaggcgt acgcggcgag ggcggtattc tgttgaatgc cgacggcgaa cgctttatgg
    14101 aacgctatgc gccgaccgta aaagacttgg cttctcgcga cgttgtttcc cgcgcgatgg
    14161 cgatggaaat ctacgaaggt cgcggctgcg gtaaaaacaa agaccatgtc ttactgaaaa
    14221 tcgaccatat cggcgcagaa aaaattatgg aaaaactgcc gggcatccgc gagatttcca
    14281 ttcagttcgc tggtatcgat ccgattaaag acccgattcc cgttgtgccg actacccact
    14341 atatgatggg cggtattccg accaactacc atggcgaagt tgtcgttcct caaggcgacg
    14401 aatacgaagt gcctgtaaaa ggtctgtatg cggcaggtga gtgcgcctgt gcttccgtac
    14461 acggtgcgaa ccgcttgggt acgaactccc tgctggactt agtggtattc ggtaaagctg
    14521 ccggcgacag catgattaaa ttcatcaaag agcaaagcga ctggaaacct ttgcctgcta
    14581 atgccggcga actgacccgc caacgtatcg agcgtttgga caatcaaact gatggtgaaa
    14641 acgttgatgc attgcgccgc gaactgcaac gctccgtaca attgcacgcc ggcgtgttcc
    14701 gtactgatga gattctgagc aaaggcgttc gagaagtcat ggcgattgcc gagcgtgtga
    14761 aacgtaccga aatcaaagac aagagcaaag tgtggaatac cgcgcgtatc gaggctttgg
    14821 aattggataa cctgattgaa gtggcgaaag cgactttggt gtctgccgaa gcacgtaaag
    14881 aatcacgcgg tgcgcacgct tcagacgacc atcctgagcg cgatgatgaa aactggatga
    14941 aacatacgct gtaccattca gatatcaata ccttgtccta caaaccggtg cacaccaagc
    15001 ctttgagcgt ggaatacatc aaaccggcca agcgcgttta ttgatgcgtt ttcagacggt
    15061 cttcgcctca aaggtcgtct gaaatctaac catacccaca ttgaactgct tgaatttata
    15121 atacaaaatc attgggcagt tgatgagaaa aggaacactt ctcatggaaa aaatgagttt
    15181 tgaaatttac cgttacaacc cggatgttga tgccaagcct tatatgcagc gttacgagtt
    15241 ggaattggaa ccgaccgacg tgaaactttt ggatgctttg gtacgcctga aagcacaaga
    15301 cgataccttg tctttccgcc gctcctgccg cgaagggatt tgcggttctg acggtatgaa
    15361 catcaacggc aaaaacggct tggcgtgttt gaccgatctg cgtggcttga aacagccggt
    15421 taaaattcgt cctctgccag gtctgcctgt tatccgtgac ctgattgtgg atatgaccca
    15481 gttcttcaaa caataccatt ccgtcaaacc ttatgttgtc aacgataatc cgattgatgc
    15541 ggacaaagag cgtctgcaaa ctcaggaaga gcgtaaagag ttggacggtt tgtacgagtg
    15601 tattttgtgc gcctgctgtt cgactgcctg cccgtcattt tggtggaacc ctgataaatt
    15661 cgttggtccg tccggtttgc tgaatgctta ccgtttcatt gcggacagcc gtgataccat
    15721 cactaatgag cgtttggata atctgaacga cccataccgt ttgttccgtt gccacaccat
    15781 tatgaactgc gtagacgtat gtcctaaaca cttgaatccg acccgagcca tcggtaagat
    15841 taaagagatt atgttgaaac gggccgttta agaaatgatg gtttttgacg atattgccaa
    15901 acggaaaatc cgttttcaaa cccgccgggg attgttggaa ttagatttaa tcttcggcag
    15961 gtttatggaa aaagaattcg agcatttgag cgataaagag ctgtccgagt tttccgaaat
    16021 ccttgaattt caagatcaag aattgcttgc cttgattaac gggcattcgg aaacggacaa
    16081 agggcacctt atcccgatgc ttgaaaaaat cagacgggca tgaggcatct gaaatctgcc
    16141 tgcaggcaga tttcaaaatg caaaagccgt ctgaaggcaa agaacgtgct gcggatgcag
    16201 taacgtgggt tataacttgc aaaggagcaa taatatgtcc aaatcaatca aactcaacgt
    16261 accgggtcag gcaggtttgg agctgccggt attggaagcc agcatcgggc acgatgtggt
    16321 tgacattcgg gggctgacaa aaaatacagg tttgttttcc ttcgaccccg gatttgtttc
    16381 aaccgcaagc tgtgagtcta aaattactta catcgacggc gatcaaggct tgctttatta
    16441 tcgcggatac cccatcgagc agctggccga aaagtccgat tatttggaag tctgctacct
    16501 gttgatttac ggcgaactgc cgactcccga gcaaaaggca gaatttgaca ataccgtccg
    16561 ccgccacacg atggtgcatg aacagctgac ttggttcttc cgggggttcc gccgcgacgc
    16621 gcatccgatg gcgatgatgg tcggcgtggt cggtgcgctg tctgcgttct accaagacag
    16681 cttggacatt agcaatcccg aacaccgcaa aatcgcgatt taccgcctga tttccaaaat
    16741 cccgaccatt gcggcaatgt gctaccgcta ttcaaacggt ctgccgttca attatccgaa
    16801 gaataatctt tcttattccg aaaacttcct tcatatgatg ttcgccacgc cgtgtgaaga
    16861 ctacaaaccc aatcccgttt tggcacgcgc gctcgaccgc atctttattt tgcatgccga
    16921 ccacgagcaa aacgcctcaa cttcaaccgt ccgtctggca gggtcttcgg gtgcgaaccc
    16981 gtttgcctgt attgctgccg gtatcgcctg cctgtggggt gcttcgcacg gcggtgcgaa
    17041 cgaagccgtg ttgaaaatgt tggacgaaat cggcgatgtg tctaatgttg ccgcatacat
    17101 ggaaggtgtg aaacaacgca aataccgtct gatgggcttc ggtcaccgcg tgtaccgcaa
    17161 tatggatccg cgtgccagca ttatgcgcga aacctgctat gaagttttga aggaattggg
    17221 cttggaagac agtccgaaat tcaaactggc gatggaattg gaacagattg cgctgaaaga
    17281 cccgttcttt atcgaacgca aactgtatcc aaacgtcgat ttctattccg gcatcgtcct
    17341 gtccgcgctg ggcatcccga ccgaaatgtt taccgtcatc ttcgccctgt cgcgcagcgt
    17401 gggctggatt tcgcactggc acgagatgat tagcgatcct tcgctgaaaa tcggccgccc
    17461 gcgccagctt tataccggtt cggaacgccg cgattatgtg ccgccaggcg agaggtaaca
    17521 tacattaata tattgtcaaa caggcaatat cagagaaccg gattgtttcc cgaatccgtc
    17581 tgattgtagt cggatgaaat caagacaagc aatccggttt aaaatagggt agaataaaat
    17641 gtcttttcag acggcatcag tttagccgtc aggacgcgga cttctaccct ttgtttatat
    17701 tttaaagaaa agagcgcacg ccatgatgga cgaaaaactc aatttctctt acctgttcgg
    17761 ttcgaacgca ccctacattg aggaattgta cgaggctttt ttggaaaacc ccgatgcggt
    17821 tgatgaaaaa tggaagcagt atttcaccga tttgagcaaa cagccgggga cggttgctgt
    17881 cgatgtcgca cacacaccga ttcgcgaatc atttgttact ttggcgaaaa agaaaattgc
    17941 atctgccgtt gcgggcggtg cggatgaggc aatgctgaaa aagcaagtca gcgttttacg
    18001 gctgatttcc gcctatcgta tccaaggcgt gggtgcagcc caacttgatc cgctcaaacg
    18061 tatccccccg cgcgatattg aagccctcga tccgaaattc cacggtctgt cagatgccga
    18121 tatggcgctt caattcaata tgggcgaggg cgattttgcc aatcgcggca aactgccttt
    18181 gtcccaaatc atcagcaacc tcaaacaaac ctactgcggc cacatcgcat tggaatatat
    18241 ctatattccc aataccgaag agcgccgctg ggtacgcaat tattttgaaa gcgtattgtc
    18301 cacaccgcat tacaatgccg atcaaaaacg ccgtatcttg aaagagatga ctgctgccga
    18361 gactttggaa cgttatctgc ataccaaata tgtcggtcag aaacgtttcg gtgtcgaagg
    18421 cggcgaaagc gcgattgccg gtttgaacta cctgattcaa aacgccggta aagacggcgt
    18481 ggaagaagtc atcatcggta tggcgcaccg tggccgtctg aatgttttgg tgaacatttt
    18541 gggcaaaaaa cccggcgatt tatttgccga atttgaaggt cgtgccgaaa tcaaactgcc
    18601 cagcggcgac gtgaaatacc atatgggctt cagctccgat attgctaccc cgcacggccc
    18661 gatgcatgtt tctttggcgt tcaacccgtc acacttggaa atcgtcaacc cggtggtgga
    18721 aggttctgcg cgcgctaaac aaaaacgttt gggcgaaaac ggccgcgaca aagtcttgcc
    18781 ggtattgatt cacggcgact ccgcatttat cggtctggga gtcaaccaag cgacattcaa
    18841 cctgtctaaa acgcgcggtt ataccaccgg cggtacggtt catatcgtca tcaacaacca
    18901 aatcggcttt accacttccg atatccgcga tacccgttca accgtacact gtaccgatat
    18961 cgcaaaaatg gtttccgccc cggttatcca tgtgaacggc gatgatcccg aacgcgtttg
    19021 ctttgctatc caagccgctt tggattaccg caaaaaattc cataaagaca tcgtgattga
    19081 cgttgtctgc taccgtaaat ggggtcacaa cgagggcgat gatccgacct tgacccaacc
    19141 gatgatgtac aaaaaagtat cgcaacatcc tggtgcgcgt gctttgtaca ccgagcaact
    19201 gattgccgaa ggtgtggtaa ctcaagccga ggcagacggt tacatccaag cctaccgcga
    19261 tgctttggac aaaggcgaac acgttgagca aacaacgttg agcaatttcc aacgcacaca
    19321 aatcgattgg agcaaatacc aaggtaaaga ttggcgcgaa cacatcgaaa ccggtttgcc
    19381 tgccgcggat attgagcgtc ttaccgagaa atttaccgcc gtaccggaag gctttgccct
    19441 gcatccgact gcaaaacgtg tgattgaagc gcgtaaagcc atggcatccg gcaaacaagc
    19501 catcgactgg ggtatggccg aaaccttggc atacgcaagc cttgtaacca aaggtcatgg
    19561 cgtgcgtatc tccggtgagg actccggccg cggtacgttc tcgcaccgcc acgcagtatt
    19621 gcacgatcaa aaacgcgaaa aatgggacga cggtacttat gttcctctgc gacatatggg
    19681 cgaaggaatg ggcgagttcc tggttatcga ctccattttg aacgaagagg ccgtgatggc
    19741 gttcgagtac ggctttgcct gctccgctcc tgacaagctc accatttggg aagctcaatt
    19801 cggtgacttc gccaacggcg cgcaagtgac gattgaccaa ttcctgtctt caggcgaaac
    19861 caaatggggt cgcttgtgcg gtctgaccac catcctgccg cacggttacg acggtcaagg
    19921 ccctgagcac tcttctgcac gcgtagaacg ctggttgcaa ctgtgttctg agaacaatat
    19981 gcaagtcatc atgccatctg aagcgtcgca aatgttccac ctcttgcaac gtcaagtctt
    20041 aggttcatac cgcaaaccgc tggtaatttt catgtccaaa cgcctgttgc gattcaaagg
    20101 ggcaatgagc ccgctggaaa acttcaccga aggttcgact ttccgtccgg ttatcggcga
    20161 taccgccgaa cgcgcaagca acgacagcgt gaaacgcgtg gtattgtgtg ccggtcaggt
    20221 ttactatgac ttggaagccg gtcgagccga acgtaaactg gaagatgatg ttgcgattgt
    20281 ccgcgttgag cagctgtatc cgttcccata cgatgaggtt aaagccgaac tggcgaaata
    20341 tccgaacgca aaatctgtgg tttgggcgca agaagagccg aaaaaccaag gcgcgttcta
    20401 ccaaatccgc caccgcatcg aagatgttat tagcgaagag caaaaactgt cttatgccgg
    20461 tcgtccaagc agcgcatcgc ctgcagtggg ctactcaagc aaacacattg ctcaattgaa
    20521 acaattggtt gaagacgctt tggcattgta aaccaagtag cattccgtct gagtctgctc
    20581 agatggaatg cccatatgca gaattaaaaa cacacaacag gccgtctgaa aaggccattg
    20641 gagacacaaa atgattattg atgtaaaagt acctatgttg tctgaaagcg tatctgaagg
    20701 cacgctcttg gaatggaaga aaaaagttgg cgaagcagtt gcccgtgacg aaatcctgat
    20761 cgatatcgaa acggacaaag tggttttgga agtaccttct ccacaagccg gcgtattggt
    20821 tgaaatcgta gcgcaagacg gtgaaaccgt tgttgccgac caagttttgg cacgcatcga
    20881 tacagctgct actgccgctg ccgaagcacc cgtagccgct cctgccgaag ccgccccggc
    20941 tgccgctcct gctgccgctc ctgctgctgc acaaaacaac gccgctatgc ctgccgccgc
    21001 caaactggct gccgaaaccg gtgttgacgt gaacgcattg caaggttccg gccgtgacgg
    21061 tcgcgtattg aaagaagatg tacaaaatgc cgctgccaaa cctgccgcag ccgctgctcc
    21121 tgctgttaca cttcctgccg gcgcacgtcc cgaagaacgc gtaccgatga gccgcctgcg
    21181 tgcccgtgtt gcagaacgtc tcttggcttc tcaacaagaa aacgccattc tgactacatt
    21241 caacgaagtc aacatgaagc caatcatgga cttgcgtgcg aagtacaaag aaaaattcga
    21301 gaaagaacac ggcgtaaaac tgggctttat gtccttcttc gttaaagccg cggttgccgc
    21361 cctgaaaaaa tatccggttg tgaatgcttc tgttgacggc aaagacatcg tgtatcacgg
    21421 ctacttcgat atcggtatcg ccatcggcag cccacgcggt ttggttgtac caatcctgcg
    21481 cgatgccgac caaatgagca ttgccgacat cgaacaagca attgttgatt acgcgaaaaa
    21541 agccaaagac ggcaaaatcg ctatcgaaga tctgaccggc ggtacattca gtattaccaa
    21601 cggcggtact ttcggttcta tgatgtccac cccgatcatc aatccgcctc aatctgcgat
    21661 tttgggtatg cacgccacta aagagcgcgc tgtggttgaa aacggccaag ttgttgtccg
    21721 tccaatgatg tatctggctc tgtcttacga ccaccgtatc attgacggcc gcgaagctgt
    21781 attgaccttg gtagccatta aagacgcgtt ggaagaccca gcccgcctgt tgttggatct
    21841 gtaatcgttt cagacggcct tttatttgtt aatgaaaagg ccgtctgaat ttttatagtg
    21901 gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt actgcctgcg
    21961 gcttcgtcgc cttgtcctga tttaaattta atccactata tttagatgta gcgtaatgta
    22021 gtatcgtgct acaataggct caacgaacga ttgaggtcgt ctgaaacatt tgattcgaat
    22081 gaatcggcag atatggactt tcagacggct ttttcttaaa accatcaaaa cgcagtcatt
    22141 caaaataaaa aagaaacgaa aagtatcgtt tttattttga gatactgcta aaagcaaagg
    22201 atgacacgat gtctcaatat gatgtagtag tgattggtgc aggcccgggc ggatacgttg
    22261 ccgccatccg tgccgcacaa ctgggtttca aaactgcctg tgtcgatgca ggcgttaaca
    22321 aagcaggcaa tgcccctgca ttgggcggta cttgcttgaa cgtaggttgt atcccttcta
    22381 aagccctgtt gcaatccagc gaacatttcc acgctgcgca acacgatttt gccgaacacg
    22441 gtatcactgt cggtgacgta aaattcgacg cggccaaaat gattgagcgc aaagatgcca
    22501 tcgtgaccaa actgaccggc ggcgtgaaat tcctgttcca aaaaaacaaa gtaaccagcc
    22561 tgttcggtac ggcttccttt gccggtaaaa atggcgatgc ttaccaaatc gaagtcgata
    22621 acaaaggaga gaaaaccgtt atcgaagcca aacacgtcat cgtagcgacc ggttccgtac
    22681 cgcgtccgct gccacaagtc gctatcgaca atgtgaacgt attggaccac gaaggcgcat
    22741 tgaacctgac cgaagtacct gccaaactcg gcgtgatcgg ttccggtgtg attggtttgg
    22801 aaatgggttc cgtatggaac cgcgtgggtg cggaagttac cattcttgaa gccgcgccga
    22861 ccttcctggc tgccgccgac caacaaatcg ccaaagaagc cttcaaatac ttcaccaaag
    22921 agcaaggtct gagcatcgaa ttgggcgtga aaatcggcga catcaagtct gaaggcaaag
    22981 gtgtttccgt tgcttacgaa actgctgctg gcgaagccaa aaccgaagta ttcgacaaac
    23041 tgatcgttgc catcggccgt attccaaaca ccaaaggcct gaacgcggaa gccgtaggct
    23101 tggaaaaaga cgagcgcggc tttatcaaag tagatggcga atgccgtacc aacctgccta
    23161 acgtatgggc aatcggcgac gtggttcgcg gcccgatgtt ggcacacaaa gccagcgacg
    23221 aaggcgttgc cgttgccgaa cgcattgccg gtcaaaaacc gcatatcgac ttcaacaacg
    23281 taccgttcgt gatttacacc gatcctgaaa tcgcttgggt gggtaaaacc gaagagcagc
    23341 tcaaagccga aggcgtggag tacaaaaaag gtacttcagg ttttggtgcg aatggtcgcg
    23401 cattggcaat gggcaaagcc aaaggtacgg ttaaagtgtt ggcagatgcc aaaaccgacc
    23461 gcatcttggg cgtacacatg attggtccgg ttgtcagcga attggttacc gaaggcgtga
    23521 ctgcgctcga attcttcgcc agcagcgaag acatcgcccg cattatccat gcccacccaa
    23581 ccttgtccga agtggttcac gaagctgcat tggcggccga caaacgcgct ttgcacggtt
    23641 gatagacatt aaggccgtct gaaacttttc agacggcctt aaggcctccg acaaattgaa
    23701 tgttccgaga gcttcgtttt ctgatttata attccgtcag acaaacaaac agcatttaca
    23761 ttcattatga acaaagaaat agtcggtatt ttctttatac cggcgggcat catcagcatg
    23821 tgtatggccg cattgtggca gatgtatgtg atgatgaccg aaacttatac gctcaaccgt
    23881 ttcaaagata aagaattggt ttggcgcgtg gcattgttgt ttatcagttt cagccttgcc
    23941 gtttatctgc tctgcccgaa ttcgcgtaaa aaaggcatcg tcttttttat tctcggggga
    24001 ggcggtgcag ccatgtatct gctggcgcgg atgtggttgc ctttcagcaa gtgaaacgac
    24061 gattttccga ccgccgaaag gtagtctgaa acgcacgggc ttgccatttg gaggcaaact
    24121 cgggggcatt ccactaatct aaaggagaaa tccatgaatt tacacgagta tcaggctaaa
    24181 gaactgctgg ctggctacgg tttgcccgta caaggcggta ttttggcaca caacggcgaa
    24241 gaagccgctg cagcttacga caaattgggc ggcaaattcg ctgttgtcaa agcacaagta
    24301 cacgccggcg gccgcggtaa agcgggcggc gtaaaagtcg ttaaaagccg cgaagaagct
    24361 aaagaagtgg ctgaaagcct gattggcacc aacttggtaa cttaccaaac tgatgccaac
    24421 ggccaacctg tcaacagcgt tttggtttgc gaagacatgt atccggttca aaccgagctg
    24481 tacttgggcg cagtggttga ccgttctacc cgccgcatta cattcatggc ctctaccgaa
    24541 ggcggcgtgg aaatcgaaaa agttgctgcc gaaactcctg aaaaaatctt caaagtaacc
    24601 gttgatccgc tggtcggcct gcaaccttgc caagctcgcg aagttgcgtt ccaactgggc
    24661 ttgaaagaca aacaaatcaa cgagttcgtc aaactgatga ccggtgcgta caaagcgttt
    24721 gtcgaaaatg acttcgccct gtttgaagtc aacccgctgg cagttcgcga aaacggcgcg
    24781 ctcgcctgcg tggacggcaa aatcggcatc gacagcaacg cgctctaccg cctgccgaaa
    24841 atcgccgaat tgcgcgacaa atctcaagaa aacgaacgcg agttgaaagc ttctgaattt
    24901 gacctgaact atgttgccct ggaaggcaac atcggctgta tggtgaacgg tgccggtttg
    24961 gcgatggcca ctatggacat catcaaactg aaaggcggcc aacctgccaa cttcttggac
    25021 gttggcggcg gcgcaaccaa agaccgcgtg gttgaagcgt tcaaactgat tctggaagac
    25081 aaatccgttc aaggcgtatt gatcaacatc ttcggcggta tcgtacgttg cgacatgatt
    25141 gcggaagcca tcgtggcagc cgttaaagaa atcaacgtcg acgttcctgt cgttgttcgt
    25201 ttggaaggca acaacgccga actcggcgcg aaaatcctga acgaatcagg tctgaaactg
    25261 acttctgcag acggcctgaa tgacgcagcc gaaaaaattg tcgcagccgt aaacgcctaa
    25321 ggagaaaaga atgagcgtat tgattaataa agacaccaaa gtattggttc aaggtttcac
    25381 cggtaaaaac ggtactttcc actccgaaca agctctggct tacggcacta aagttgttgg
    25441 cggcgttacc ccgggcaaag gcggtcaaac ccacctgaac ctgcctgtat tcaacaccat
    25501 gaaagaagcg gttaaagaaa ccggcgctga cgcatctgta atttacgttc ccgctccgtt
    25561 tgtattggat tctatcgttg aagcagttga ttcaggcgta ggcttggttg ttgtgattac
    25621 cgaaggcgtg cctactctgg acatgctcaa agccaaacgt tacttggaaa ccaacggcaa
    25681 cggtacccgc ttggtcggcc ctaactgccc gggcgtgatt actccgggtg agtgcaaaat
    25741 cggcattatg ccgggccaca tccacactcc gggccgcatc ggtattattt cccgttccgg
    25801 tacattgact tacgaagctg tggcacaaac caccaaactg ggtttgggtc aatcaacctg
    25861 tatcggtatc ggcggcgatc cgattccagg tatgaaccaa atcgacgcac tgaaactttt
    25921 ccaagaagac ccggataccg acgccatcat catgattggt gaaatcggcg gtactgcaga
    25981 agaagaagca gccgaataca tccaatccaa cgtaaccaaa cctgttgtag gttacattgc
    26041 tggtgttacc gctcctaaag gcaaacgtat gggccacgcc ggtgcgatta tttccggcgg
    26101 taaaggtact gccgaggaaa aattcgccgc tttcgaaaaa gccggtatcg cttacacccg
    26161 cagccctgcc gagttgggca ctaccatgct ggaagtgttg aaaacaaaag gtttggcata
    26221 atcaggtttg acaactgatt gaacatcaaa tgccgtctga aaccggaaat cgggtttcag
    26281 acggcatttt gtttgtcatt tcaaaaagag gcagcctcaa catacccaca ttatttttgc
    26341 cctttgaggc aggcagaata tttatcggtc aaacaggagc aaacccgtaa gtcaggttgc
    26401 acacgccttg tttgaagttg gggaatgata actatcgcat ttgaaaagac tgatattatt
    26461 ttttgcagga ttctgtttta gcacgcgaca ttttttgttt gataggattg gaatggatgt
    26521 agtccgcgca acgctgcaaa tccgtttcgt cgcgcacggt gtgtccgtaa aaacgccgtt
    26581 gccaaatacc gcgttcgtgc tgccgctgtt tgccggcgga taagttttta gtataagggg
    26641 aatatgcgga gaatttggtt ttaatcagcc gccggagtag ggaataatcc gcatcgtcgg
    26701 gcggcggcgt ccaaatggcg tggatatggt tcggcagcac gcatacggcg acggtttcaa
    26761 aggaatattg tttttgcaca tccatataag ccgcccgcga agtcgatatg ttcgacaagc
    26821 aggcgcgatt tcgggtcggc gagtttgacg gtgaaaaaga atgtgccgcc ggcaatgaag
    26881 tttctgcgat aacgcgccat aggatttttg tttttggttt gagcgttgga gaaggaatgg
    26941 cttgttgttt aaggttttta acttcgttga agctagcgct ttcagacgac attggaggcc
    27001 gtctgaaagc gctagcttca acgaagttaa aagttgaaac ggcaaaccgt caatcaagca
    27061 accgcgtgcg tgcgtaccgc acacacccta cacgttgatt ttgaagttcc gtgtaattca
    27121 caggtagggt gtgtggcgca gccacgcacg cggtgttggg tatgcaggct acggcttgct
    27181 gctgcagaaa ctcctgaaga aaaagccaaa gacgccgctg aagaggctaa agaagaagca
    27241 aaagacgcag cggaagatgc caaagaagct gctgaagacg ctaaagaaga agctaaataa
    27301 ttcggatagc tttacagaaa aacggattcc aatgattggg aatccgtttt ttgttttgtg
    27361 agttagggta gttgttttag ataaagcttt ttcagttttc agacggccat ttttcatgcc
    27421 gtctgaaagt gttagtttta actaagttca atttacaaca gaaagccatc aatcaaacaa
    27481 ccgcgtgtgt ggcgcagcca cgcacgcggt cggttggata tgtaggctac ggcttgctac
    27541 gttttttatc aaaggcaggc gctaagtgtt cagcattgtt gatgccgtat aaaactaaat
    27601 tatctaacag ccataatcta aaagcaggat catcgatatg atgatctgac gaacaatcga
    27661 ctcgccattg ctgtaaaaca tatccgatat ttgaagctct aagcgccaat tctaattttc
    27721 catttgtcat tccaaagtcc atttcaatca cttttttagc ttcatcatga ttaggatggg
    27781 gaaccaactc gattttaaca attctattcc aatcaatatc attgttaatc gtttcatttt
    27841 cttcaatatt ttcagctaat aaaatatttg cttgaattat tcgtgttagg acaaaatctg
    27901 caaatcggtt ttttgttcta tcgaatgccc gtaaatgcca tcgaaatccg tcattaacta
    27961 gagcaagagg cacaataact ttctcagata atccagatga aattgaatga tatgagattt
    28021 ttagaggttt ttttaaggat aatgctttcg atattttaga aaagattaag acatcaggat
    28081 ttattaaaac aggaggtaaa tcaaacggaa gaagaggttg atgttgggaa tcaatatcaa
    28141 atattaaact gccattaaga attttaattg cattttcaaa tgaatggggg aattgaggtt
    28201 caaagtcttc agtagagagg taaaatttac ttttggtatc aaaacggaga tttttcgaaa
    28261 aactagagca atatagcgca aaatccctag tagcaacagc tggggaaatt tgaaatgcct
    28321 gcatcaaatc ttttctattt atctttccta gaaagcatag atagaaatca ataaatataa
    28381 tcctattgcg ttgagattgg ttcaactctg gcataagggg ctttatatgg gaaggagcga
    28441 ttgttttcat aattgcatta tattccatca atctttttca tggcaatatg aattcaaaaa
    28501 aattgcttga cgtaatcaaa aagagtgcat ataatgcaaa aagctacttc atagtagctc
    28561 tctgacttaa aatttctgtt atgtaatttt ttataggatt tatttatgaa aataaaattt
    28621 gctcacttaa gacatccagc cgtaggtgga ggatatatta actttgctgt ttttgatgct
    28681 aaatcaacta atggtaacca tagtgtcgta ctgaaggaat tgaccactga agctcgtaga
    28741 aatggattaa aaattgacca atctgcgtta atatttgaac aacatgggcg tacacaattc
    28801 tatggagaca aaaatctagt agatttttta tctgcaaatt ggtatccgca atggactcat
    28861 actttaacta tttaaattat ggagatgata atgacgaaca aaatcaaacc tggtcaaaat
    28921 acaggaaata acggtggaat ttatcaagaa attggttcac gaggtggtcg aaaagataac
    28981 tacgcaacca ttaaagataa tcaaaaagca ccacccactc aaaatccagg aagctcatgg
    29041 attcaaatta aaagaactcc agatagctcc agataataag gatagttcta atgtttttag
    29101 gtatttttat agctttatca ggcattctta cattattaga aatgtttaat gttatttctt
    29161 cggatatgaa atgggggatg cctttaatgt tcacctgttt gggatttagc attatttttg
    29221 atagcttcaa agccaaacaa aatcgaaaac agaaggaata aaaaaacggg atggtttaaa
    29281 ccatcccgtt ttttaaagta tattcttcaa atacttccca gtataactcc ccttaacctt
    29341 cgccacttcc tccgggctac ctgaagcaat aatcctccct ccgccgtcgc cgccttctgg
    29401 ccctaagtcc acaatccaat cggcggtttt aatcacatcc agattatgct cgataatcac
    29461 tatcgagttg cctttgcctt tcagacggtc tatgacttcc agcagcaggg cgatgtcggc
    29521 gaagtgtagg ccggtggtgg gttcgtcgag gatgtagagc gttctgccgg tgtcgcgttt
    29581 ggagagttcc aaggcgagtt tgacgcgctg ggcttcgccg ccggagaggg tggtggcgga
    29641 ttggccgaga cggatgtagc ctaagcctac gtccatcaag gtttgcagtt tgcgcgatac
    29701 ggtggggacg gcgtcgaaaa attcgcgggc ttcttcgacg gtcatgtcga ggacttggct
    29761 gatgtttttg cctttgtatt ggatttcgag ggtttcgcgg ttgtagcgtt tgccgtggca
    29821 gacttcgcag ggaacataca cgtcgggcag gaagtgcatt tcgactttaa tcacgccgtc
    29881 gccttggcag gcttcgcagc ggccgccttt gacgttgaag gagaatctgc cgacgttgta
    29941 gccgcgttcg cgcgagaggg gtacgccggc gaagagttcg cggatggggg tgaacaggcc
    30001 ggtgtaggtg gcggggttgg agcgcggggt gcgaccgatg ggggattggt cgacgttgat
    30061 gactttgtcg aggtgttcga ggccgcggat gtcgtcgtat ggggcgggtt cttcttgggc
    30121 gcggttgagt tcgcgggcgg taattttggc gagggtgtcg ttaatcaggg tggatttgcc
    30181 gctgccggat acgccggtga tgcaggtaat caaaccgagc ggcagctcaa gggtaacgtt
    30241 tttgagattg ttgccgcgtg cgcctttgag gacgagcatc cggtcgggat tgacgggcgt
    30301 gcgttcagac ggcacggcaa tggatttttt gccgctgagg tattgtccgg taacggagtt
    30361 ttcgcattgg gcgacgtttt cgggcgtgtc ggcaatcagt acgttgccgc cgtgctcacc
    30421 cgcacccggg cccatatcga ccacgaaatc ggcttcgcgg atggcgtctt cgtcgtgttc
    30481 gaccacaatc acgctgttgc ccaaatcgcg cagtcgtttg agggtggcca gcaggcggtc
    30541 attgtcacgc tggtgcaggc cgatggaggg ttcgtccagt acatacatca cgccggtcag
    30601 gccgctgccg atttggctgg cgaggcggat gcgctgggct tcgccgccgg agagggtttc
    30661 ggcggagcgg cttaaattca ggtaatccag cccgacgtta atcaggaagc cgaggcgttc
    30721 ggtgatttct ttgaggattt tttcggcgat ttgttttttg ttgccgtcta aatccagtgt
    30781 ttcaaagaat tggtgggttt tggtgagcgg ccaggcggaa acttcgtgca acggctcacc
    30841 gctgacgtaa acgtagcggg cttctttgcg caaacgtgcg ccgccgcagc tcgggcaggc
    30901 gcggtggttt tggtattcgc gcagtttttc gcgcacggtt tcgctgtcgg tttcgcggta
    30961 gcggcgttcg agattgggga tgatgccttc gaaggcgtgg ctgcggttga aggtggtgcc
    31021 gcgttcggac aggtaagtga aatcaatgac ttccttgccc gagccgtgca acacgacttt
    31081 tttgattttt gcaggtaggg tttcccaagc agcctgcaca tcaaaaccgt aatgccgtgc
    31141 cagcgactga atcatttgga aatagaactg gttgcgcttg tcccagccgt caatcgcgcc
    31201 cgttgccaac gacaattcgg gatgggcgac tactttttcg ggatcgaaga aattggtgtt
    31261 gcccaagccg tcgcaagtcg ggcaggaacc catcgggttg ttgaacgaaa agaggcgcgg
    31321 ctccaattcg ggcaggctgt acgaacatac ggggcaggcg aaacgcgcgg aaaaccaatg
    31381 ttcttcgccg ctgtccattt ccatcgccag cgcgcgctcg ttgccgtggc gcagcgcggt
    31441 ttcaaaactt tccgccagcc gctgcttgat gtccgccttc actttcacgc ggtcgatgac
    31501 cacgtcgata ttgtgtttga tgtttttttc cagcttcggc acttcgtcaa gctgataaac
    31561 ttcgccgtcc acgcacacac gggcaaagcc ttgcgcctgc aagtcggcaa agaagtcgac
    31621 aaactcgccc ttacgctcac gaactgccgg cgcaagaatc atcacgcgcg tatcttccgg
    31681 cagttttaat acggcatcga ccatctgaga tacggtttgg ctcgacagcg gcagattgtg
    31741 ttcggggcaa tacggtgtgc cgacgcgggc gtacaaaagg cgcaggtagt cgtggatttc
    31801 cgtaaccgtg ccgacggtgg aacgcgggtt gtggctggtg gatttctgct cgatggaaat
    31861 cgcaggcgag aggccttcga tcaaatcgac atcgggtttg tccatcatct gcaaaaactg
    31921 ccgcgcatag gcggaaaggc tctcgacata acgccgttgc ccttcggcat acagcgtgtc
    31981 aaacgccagc gacgacttgc cgctgcccga caatcctgtt accaccacga gcttgtggcg
    32041 cggaatgtcc aaatcgacgt ttttcaaatt atgcgtgcgc gcgccgcgga tgcggatggt
    32101 gtcgttgtcg tgcgaatgtt ggggatgatg cttgcacata atggatgccg cctgaaaaat
    32161 aaaggaaaac tggtattgta gcactttctc ggatgccgtc tgaagccgcg ttcagacggc
    32221 atttgccagc ggagtacggc agattccgct ataatgtcgg caattttaac ccgcttgaac
    32281 aaaaggatga caaatgaacc gtctttaccc ccacccgatt atcgcccgtg agggctggcc
    32341 gattattggc ggcggtttgg ctttgagcct gctggtgtcg atatgttgcg gctggtggtc
    32401 tttgccgttt tgggtgttta ccgtatttgc attgcagttt ttccgcgacc ctgcgcgtga
    32461 gattccgcta aatcctgaag cggtgttgag cccggttgac ggccgtatcg tggtggtcga
    32521 acgcgcacgc gatccgtatc gtgatgtcga tgctttgaaa atcagtattt ttatgaacgt
    32581 gttcaacgtg cattcgcaaa aatcgcctgc cgattgtacg gtaacgaaag tggtctataa
    32641 caaaggcaaa ttcgtgaatg cggatttgga caaagccagc acggaaaacg aacgtaatgc
    32701 ggtgttggcg actacggctt caggtcgtga aattactttt gttcaagtgg ccggtttggt
    32761 ggcgcgccgt attttgtgct acacccaagc aggtgcgaaa ctgtcccgcg gcgaacgtta
    32821 tggctttatc cgcttcggtt cgcgcgtgga tatgtatctg cctgtcgatg cgcaggcgca
    32881 agtggcgatt ggcgataaag taaccggcgt cagcactgta ttggcgcgtt tgccgctgac
    32941 tgcgccgcaa actgaatctg agcctgcttt acaaactgct ccggttgaaa cagcggcaaa
    33001 cccatctgcc gaacaacggc aaatcgaggc agcggcggct aagattcagg cggctgtgca
    33061 agatgtgttg aaagattaat tttgcggact gaaatagaaa atatcagtac catcattcac
    33121 acgaatgagg aagtttggtt ttttgaattt ttgctaatgt tcacaccgtc attcccacga
    33181 aagtgggaat ctagaaactt aacgttacga cgatttatcg gaaacgactg aaaccggacg
    33241 gactggattc ccgcctgcgc gggaatgacg acttattagt tacctaacac ttaaaaaaca
    33301 gaaacctttc cgcgtcattc ccacgaaagt gggaatccgg gaacttaacg ttacgacgat
    33361 ttatcggaaa cgactgaaac cgaagagacc ggattcccgc ctgcgtggga acgacaactc
    33421 attagttact taaaacttaa aaaacagaaa cctttacgcc gtcattccca cgaaagtggg
    33481 aatccgggaa cttaacgtta cagtgattta tcggaaataa cagaaaccga acgaattgga
    33541 ttcccgcctg cgcgggaatg acgacttatt agttacctaa cacttaaaaa acagaaacct
    33601 ttccgcgtca ttcccatgaa agtgggaatc tagaacccaa atgctaaggc gatttatcgg
    33661 aaacggctga aaccgaagag accggattcc cgcctgcgcg ggaatgatgg gtttctgctt
    33721 ttgatttttc tgcttttgcg agaatgacgg cgtgaaagca agaatgatga aacaaagaaa
    33781 atgggaatga tggcatattg gtttgttctt tgtctttgcc atatttccta acaaattgat
    33841 taaaaagaaa aaaggttttc agaatgccgt ctgaaaacct tttttgtttg cctgtccgat
    33901 tttaaaactt cacgttcacg ccgccggtaa agctgcggcc catttgcggc gtatcagaga
    33961 ggaagctgct gtgggcgtaa acggattggt tgagcaggtt gtcggctttg acgtaccaat
    34021 tccactcgcc atagcgcgta ttgcggcggt agtttgcgcc gaggttgagc atatggtgtc
    34081 cgggcgtgcg cgtttcgtag cgggcgagtt tgttttgggc gaacacgcgg tagtagtcca
    34141 aattggcatc gatgcggtcg gtcagcgagg ctttcaggtg gacgccgagg cgcgcagccg
    34201 gaacgcgagg ggcgttttgg tcgtcctgcg cgatgaaagg acggttgccg taggcatctt
    34261 ccctgccggg tagggaaggc aggtttttca gacggcctcg tacatagtcg ccggaaacgc
    34321 cgatgcggta gcgcggtgtc ggtttgaagt agatttcgcc ttctgcgccg tagaagtccg
    34381 caccggattg gttgtagcgc acgagcttca tttcgctgtc gtcttcgatg gatttggggc
    34441 cgcgtccgtc gtttaaggtt tgggcgtaaa tgtagttgcc gaagcggttg cggtagagtg
    34501 ccagattgta ttgccagcgg tcgccttcgt agcccagcgc gagttcgata ttgttggaac
    34561 gctctttgtt gaggtgtttg ttgccgactt caaaggtgtt ggtggcgacg tgtttgccgt
    34621 gtgcgtacag ctcttgcgtt gacggcaggc gttcctgatg ggaggcggtc aggctgagtt
    34681 tgtgttgtgg cgtgaaatac cagttgcccg aaagtgcgaa tgagcgggcg gtttggcggt
    34741 gcgcgccgag gtcgggcagg ggatggttgt agtagttttc ccgatcaatc aatgctttat
    34801 cgtagcggat ggaggctttt tgtttttcca cgcgtacgcc gccttcaagc gtgaagttgt
    34861 cccagtttgc ctgttctaca ccgaaaaagc tgtaatgttg cactttattg tcaagcagca
    34921 tcggttgttt gaccgcttcg gatgtggcag ataaagcact ggatttttgt tgtaaatatt
    34981 gcacgcccca gctgcctttc agacggccta tgggttggtg gcgcaactcg atgcgggcgt
    35041 tttgcgtttg gttgttaaaa aagttttcga ccgcatcgcc tgctttttcg tcgtggcggt
    35101 agtcgttgcg gttcaagtat acgcgcaggg cttcaaaacc ggggaacggt tgcttccatt
    35161 cggcacggag ttcgtagcgt ttgttgcgca ggtctatcca cggtctgccg ctgtgggtgt
    35221 gtgcgtgtgc attatcgtcg tcgtggaagc cgcagctcaa gcccggattg tcgtaatcga
    35281 tgtcttcttc ggtcaacagg tgcggataaa gctgtaaata gcgtttgtta atcaagctct
    35341 tttgccagat gatgtcggcg tggcaatcat cgtattcgtg gctgtgggca ggcagaccat
    35401 attggtcgcg acggtcgctg tacgctacgc cgataaaacc tttttcgcca acccaagaca
    35461 gcccgatgct gcccgtttgc gaatcggcgc ggctgtcggg caggcgtttc agattgcggt
    35521 aacgcggtac ggcgtaatcg cccgatttgc ggtacaagcc ttccgtatgc aatacaaagt
    35581 ttttgcccaa gccgatattg atgcctgcgg atgtcagttt ttccaaattg ccgctgctca
    35641 aacgcaatcc ggcttcgccc gatacgccgt tttcaggcat tttttcgggg attttgccat
    35701 cggcaacatc gaccagcccc gccacattgc ccgagctgta caagagcgta accggcccgc
    35761 gcaggatttc gacctgttgc gacaaggcgg tatctaccat aatggcgtga tcgggcgaaa
    35821 aatccgccat atcgcctgtt tcgccgtgat ggttcaacac tttaatccgc ctgcctgttt
    35881 gaccgcgaat aacgggagcg gacgcgccgc cgccgtattg cgaggcgtgg atgcccggta
    35941 cgccgtctaa agcatcgccc aagttgacgg ctttttggcg caaggtatcg ccggagatga
    36001 ttttgtcgga ggcggtcgaa gtgtgcagca gccccgatgt ggcgcgcgga cggcttttgc
    36061 cgacgacgct gaccgtttcc aagtccaccg attgctcagt ttcatgcgct tgggcaagga
    36121 ggggtgtgtt gattaaaaga attgataaaa caatgggttt gagtgtggtt tgtaccattt
    36181 tggcttctcg actcatttca aaagtttgtt attatataac attgcattcc ttatatcata
    36241 agattttgag aacactcaga gggcataggc aaaagttttt caaatgacac ggttgcggca
    36301 tcgggcggtg tccatttgta tccgccgtca ttcgggggcg cggttgatgt tgacgcagat
    36361 tccgctgtgt ttgcccacta tgttcggctg cgggataatc aaaatttatg agtgcataaa
    36421 aacggcacgt tcccgaacgg tcgaaaaggt ggcaaaatgg cgtacttgtc agaacgcgag
    36481 gctctgcgcc aggttcacga gggcgcatcg ggcacgctaa gatataagag ggtatggatg
    36541 ggggtatcgg aaatgcagtt aataacaaac aaattataaa tcaataggtt aatcacaaaa
    36601 tgcttttgtt tatcgacaat tacgacagtt ttacttacaa catcgtccag tatttcactg
    36661 aattggggca ggaagttgcc gtgcgccgca acgatgatat tacgttggag gaaatcgagg
    36721 cattgaatcc gcaatatctc gttatcggcc ccggcccgtg ttcgcccaaa gaagcgggga
    36781 tttccgtggc ggcgatgcgc catttcgccg gccggctgcc gattatgggc gtgtgcctcg
    36841 ggcatcagac gataggcgag gcgttcggcg gcaggatagt ccgcgccaaa acgctgatgc
    36901 acggcaaggt gtcgcccgtg tcccattcgg gcaagggtat gtttaaaggt ttgcccaatc
    36961 cggttacctg tacgcgttat cacagcctcg ttatcgaacg cggcacgttg ccggattgct
    37021 tggaaatcac ggcttggact gcggacggcg agattatggg cgtgcgccat aaggaatatg
    37081 ccgtcgaggg ggtacaattc caccctgaag ctctgctgac cgaacacgga catgatatgt
    37141 taaacaattt tttagttgaa tttcaaaact tcaaaccgca aaaaatctga cgtgatgccg
    37201 tctgaatccc ttcagacggc attttcgtcc gaatattgaa cggaggacaa aaaatgatta
    37261 caccgcaaca ggctatcgaa cgattaatca gcaataatga gttgttttac gatgaaatga
    37321 ccgacttgat gcgccaaatg atgagcggaa aagtgccgcc cgaacaaatt gcggcgattt
    37381 tgacaggatt gcgtatcaag gttgaaaccg tttccgaaat taccgcagct gcagccgtca
    37441 tgcgcgagtt tgcgacaaaa gtgccgctgg agaatgcgga ggggctggtt gatatcgtcg
    37501 gtacgggcgg ggatggtgcc aaaaccttca atatttcgac gacttcgatg tttgttgccg
    37561 cagcggcagg cgcgaaggtt gccaaacacg gcgggcgttc cgtttcatct tccagcggcg
    37621 cggcggacgt gatggagcag atgggcgcaa acctcaacct gactcccgaa cagattgccc
    37681 aaagtatcag ccagaccggt atagggttta tgttcgcccc caatcaccac agtgccatgc
    37741 gccatgtcgc cccggtacgc cgttcgctcg gtttccgaag cattttcaac atattgggtc
    37801 cgttgaccaa tcctgcgggc gcgccgaacc agcttttggg cgtgttccac accgatttgt
    37861 gcggcatttt gtcgcgggtc ttgcaacaac tcggttcaaa acacgtttta gtcgtttgcg
    37921 gggagggcgg tttggatgaa attacactga cgggcaaaac gcgcgttgcc gaactcaaag
    37981 acggaaaaat cagcgaatac gacatccgcc cagaagattt cggtatcgaa acccgccgca
    38041 atttggatga aatcaaagtt gccaatactc aggaatcttt gttgaaaatg aatgaggtgc
    38101 tggatgggaa agaaggggct gcgcgcgata tcgtattgct taataccgcc gccgccctat
    38161 atgccggaaa tgtcgctgct tcgctttcag acggcatatc tgccgcacgg gaagccatcg
    38221 attcaggcag ggccaaatcg aaaaaagagg agtttgtcgg ttttcaacca caacaaagat
    38281 gccattttct tggaaagatg gagcttgggt gatgccgcca tgattatgga acttttgtgg
    38341 caaaacataa gcacttcacg aagagaactt accaaactgt ttttatataa aaacttgggg
    38401 ctgtactaga taaccagacc aaattcccat taactaattg tcttaaaatc tgaatttgag
    38461 attctattta aaatgccatt ggcatttctt taaatgcagc cccaaatgct ctttgggaat
    38521 gccgttaaac ttacgtaaat ggctaaattc actaatatca agcacatcat aactacgaaa
    38581 ggtatccgtg tatacaatgc catcaggctt aactttcttt cggataattg gcaacaatgt
    38641 cgctgattgc gcattaggaa caacgacggt a
//
LOCUS       DAWFJW010000007        36738 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFJW010000007 DAWFJW010000000
VERSION     DAWFJW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.007"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR19_000282"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR19_000282"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0797882.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAPAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKG
                     RKVDIIQGDGQFLDPHHLEVSLTTSEVYEQATPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIFDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMINTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKASFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1965..3569)
                     /gene="aceF"
                     /locus_tag="WGR19_000283"
     CDS             complement(1965..3569)
                     /gene="aceF"
                     /locus_tag="WGR19_000283"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0797883.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKVGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAADAAPAPAAEAPAAPATAPA
                     AAPAGGATVQVVVPDIGGHSDVDVIAVEIKVGDTVAVDDTLITLETDKATMDVPCTEA
                     GVVKAVFLKVGDKVSEGSAIIEVETAGSATAAPAPAAQAAAPAPAAAPAPTAPAAAPA
                     PAAPAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGEDVKAFVKSVM
                     QSGAGKPAAASLGGGLDLLPWPKVDFSKFGSVEVKELSRIKKISGQNLSRNWVVIPHV
                     TVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVAALKAFPEFNASLDGDNL
                     VLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGA
                     CFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHR
                     VIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WGR19_000284"
     CDS             complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WGR19_000284"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0797884.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDTQLKSSGDREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRVGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7076..8788
                     /locus_tag="WGR19_000285"
     CDS             7076..8788
                     /locus_tag="WGR19_000285"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0797885.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDAVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8851..9507
                     /locus_tag="WGR19_000286"
     CDS             8851..9507
                     /locus_tag="WGR19_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0797886.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFAAADRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WGR19_000287"
     CDS             complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WGR19_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0797887.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9965..10513)
                     /locus_tag="WGR19_000288"
     CDS             complement(9965..10513)
                     /locus_tag="WGR19_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0797888.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10515..11075)
                     /locus_tag="WGR19_000289"
     CDS             complement(10515..11075)
                     /locus_tag="WGR19_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0797889.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGKLFAGTCMISNPIENPDKR"
     gene            11403..13208
                     /locus_tag="WGR19_000290"
     CDS             11403..13208
                     /locus_tag="WGR19_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0797890.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13335..14819
                     /locus_tag="WGR19_000291"
     CDS             13335..14819
                     /locus_tag="WGR19_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0797891.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQEKTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMSGDPLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15148..17175
                     /gene="uvrB"
                     /locus_tag="WGR19_000292"
     CDS             15148..17175
                     /gene="uvrB"
                     /locus_tag="WGR19_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0797892.1"
                     /translation="MEVIRYPDSPFKLHQPFQPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEASVLRDRIRGIREGLLFGSE"
     gene            17287..17574
                     /locus_tag="WGR19_000293"
     CDS             17287..17574
                     /locus_tag="WGR19_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797893.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIEVVYVLEQQQG"
     gene            complement(17755..18471)
                     /gene="trmB"
                     /locus_tag="WGR19_000294"
     CDS             complement(17755..18471)
                     /gene="trmB"
                     /locus_tag="WGR19_000294"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0797894.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIR"
     gene            complement(18593..19996)
                     /locus_tag="WGR19_000295"
     CDS             complement(18593..19996)
                     /locus_tag="WGR19_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0797895.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EAGLSEAQVRLMYLLYASRHFEEALEWAKTTAKNNNPHGQYLLAQYCRYSTPPDFETA
                     HLFYRKAAVQGLAEAQWQLGLQYRFGQGTQTDTAQAVNHLRAAAQQGYIPACTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKYLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIASDKEKARHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQQAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGIAADYGKARKWFEQAAAQKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            20060..20161
                     /locus_tag="WGR19_000296"
                     /pseudo
     CDS             20060..20161
                     /locus_tag="WGR19_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(20220..22106)
                     /gene="uvrC"
                     /locus_tag="WGR19_000297"
     CDS             complement(20220..22106)
                     /gene="uvrC"
                     /locus_tag="WGR19_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0797896.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCSAPCVGHISEEDYHDSVREASTFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRCLQQSSQ
                     QHRIDELAKILNMNSDDLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEQVEGISKALAETIY
                     NHLH"
     gene            complement(22161..24323)
                     /locus_tag="WGR19_000298"
     CDS             complement(22161..24323)
                     /locus_tag="WGR19_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0797897.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            24463..25413
                     /gene="trxB"
                     /locus_tag="WGR19_000299"
     CDS             24463..25413
                     /gene="trxB"
                     /locus_tag="WGR19_000299"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0797898.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLETDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            25569..25937
                     /gene="rpsF"
                     /locus_tag="WGR19_000300"
     CDS             25569..25937
                     /gene="rpsF"
                     /locus_tag="WGR19_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0797899.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            25938..26240
                     /gene="priB"
                     /locus_tag="WGR19_000301"
     CDS             25938..26240
                     /gene="priB"
                     /locus_tag="WGR19_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0797900.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            26247..26477
                     /locus_tag="WGR19_000302"
     CDS             26247..26477
                     /locus_tag="WGR19_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0797901.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            26494..26946
                     /locus_tag="WGR19_000303"
     CDS             26494..26946
                     /locus_tag="WGR19_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0797902.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQVEILADARVRQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            26960..27067
                     /locus_tag="WGR19_000304"
     CDS             26960..27067
                     /locus_tag="WGR19_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0797903.1"
                     /translation="MPSETLPIIGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            27633..27788
                     /locus_tag="WGR19_000305"
     CDS             27633..27788
                     /locus_tag="WGR19_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797904.1"
                     /translation="MYAGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(27904..28710)
                     /locus_tag="WGR19_000306"
     CDS             complement(27904..28710)
                     /locus_tag="WGR19_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0797905.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGPQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLINGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(28711..29457)
                     /gene="pssA"
                     /locus_tag="WGR19_000307"
     CDS             complement(28711..29457)
                     /gene="pssA"
                     /locus_tag="WGR19_000307"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0797906.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            30040..30522
                     /locus_tag="WGR19_000308"
     CDS             30040..30522
                     /locus_tag="WGR19_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0797907.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(30574..31785)
                     /locus_tag="WGR19_000309"
     CDS             complement(30574..31785)
                     /locus_tag="WGR19_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0797908.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQIATKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLTGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            32008..34446
                     /locus_tag="WGR19_000310"
     CDS             32008..34446
                     /locus_tag="WGR19_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0797909.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            34654..35964
                     /locus_tag="WGR19_000311"
     CDS             34654..35964
                     /locus_tag="WGR19_000311"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0797910.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            36060..36674
                     /gene="clpP"
                     /locus_tag="WGR19_000312"
     CDS             36060..36674
                     /gene="clpP"
                     /locus_tag="WGR19_000312"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0797911.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
BASE COUNT         8920 a         9142 c        10050 g         8626 t
ORIGIN      
        1 ccggaagaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atatcgccgc cgttaggacc aacgataccg ccaccgatga
      301 tgcggccggt ttcggcatca aaaatcagct tggtgaaacc attgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagcagcc cacgggaagc tggctttggt gattttgcgg ccggaggctt
      421 tggcggacaa ttcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatgacgcg cgcgtcgaag taggctttgt ggccggcaca gttttcagcg gcaacgtggc
      541 cttcgtgaac agctttgtgt gccaacatcg gctgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac gttggtgcgc atttgtttgt ccacttcgat aaagccgcga tcggttacgg
      661 caacaccggc tttttccgca ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 acacggcatc gtaacgttgt ggctctttcg gcgcatttgc accttcgaag gtaacgtaaa
      781 cgccgtcttc tttaggctcg acagcaacag ttttggtgtt gatcatgatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaacaaca tccaggcgag aacccagcgt gctgtaaacc gtacccattt
      961 caagaccgat aataccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggagtcgaaa attcgaggat cttcagggat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctct ccggtaggcg
     1141 tagcttgttc gtacacttcg ctggttgtca gagaaacttc caagtggtgt ggatccaaga
     1201 attggccgtc gccttggata atgtctactt tgcgaccttt ggccatacct gccaaaccgc
     1261 cggtcagacg agaaaccacg ccatctttgt aggcgcgcag catgtcgata tcaagttcag
     1321 gttcaggata tttgataccg ttggcagcca agtggcgtac ttcatcgata acagcagcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcaa gcaaacgccg cccaaagttt
     1441 tgtaacgttc aacgatggca actttcaagc cttcatcggc agctgcaaat gcagcagaat
     1501 aaccgccagg gccgccaccc aatacaacta cgtcgtattc ggcatcggca gaaccgccga
     1561 attgagcagc ttgaggggca ggagccgctg ctttaggtgc ttcttgtgca ggagcggcag
     1621 gcgcttcggc tttaggagca gcagctgcac cttcggcctc aacaacaaca atcaaaccgc
     1681 cttcggagat tttgtcgccc actttaactt taacttcttt gactacgcct gcaacttcgg
     1741 cagggacgtc catagtcgct ttatcggttt ccaaagtaat cagtgtatcg tccacagcaa
     1801 tggtatcgcc cacatttact tcaaccgcaa taatatctac attttcgtga ccaccgatgt
     1861 cgggcacttt caattcaact aagctcatgt ctcatctttc tgaatgatgt tcaaacttcc
     1921 tttttagacg gcacagaccg ctatgccgtc tgaagccgat cgggttacag ggtaatgcgg
     1981 cggaagtctt tcaacagctt cgccaagaat acggtgaagc gcataccggc cgcaccgtcg
     2041 atgacgcggt ggtcgaagga caggctcaat gggcacatca ggcgaggagc aaattctttg
     2101 ccgttccaaa ccggtttgat ttgggatttg cacacgccca agatagcaac ttcaggagca
     2161 ttcacgattg gcgtgaagcc tgtaccgccg atgccgccca agctggaaat ggtaaagcat
     2221 gcgccttgca tttcttgcgg tttgagcttg ccttcgcggg cttttttaga caattcggtc
     2281 agctcttggc tgatttgttt caagcctttt tgatccacgt ctttgattac tggaacaacc
     2341 aagccgttcg gcgtgtctgc tgcgaaaccg atgttgaagt agtttttcag caccaagttg
     2401 tcgccatcca gagaggcgtt gaattcaggg aaggctttca gcgcagcaac agaggcttta
     2461 atgatgaacg ccaatgggga cagtttcacg ccttcgcgtt cccattcttt gttcagttgt
     2521 ttgcggaatt cttccaattc ggtcatgtcc gcttcttcgt gtacggtaac gtgaggaatc
     2581 acaacccagt tgcgggacag gttttgaccg gagattttct taatgcgtga cagttcttta
     2641 acttcgacgc tgccgaattt agagaagtca actttaggcc atggcaacaa gtccagaccg
     2701 ccgcccaaag aagcagcggc aggtttgcct gcaccgcttt gcataacgga tttaacgaaa
     2761 gctttaacgt cttcgcccat gatacggcct ttcagaccgg tacctttaac ttggcccaaa
     2821 tctacgccca attcgcgtgc cagtttacgt gcggaaggac ccgcgtgtgc tttggcaaaa
     2881 gcggcttcgt cgattttggc ggcaggagca gcaggtgcag gcgcggcggc cggagcggta
     2941 ggagccggag cggcagccgg tgcaggtgcg gcagcttgag cggcaggagc aggagctgct
     3001 gtggcagagc cggcggtttc tacttcgata atggcagagc cttcggatac tttgtcgccg
     3061 actttcagga atacggcttt aaccacaccg gcttcagtac atggtacgtc catggtggct
     3121 ttgtcggttt ccaaagtaat cagggtgtcg tcaacggcaa cagtgtcgcc aactttaatt
     3181 tcaacggcaa taacgtctac gtcagagtga ccgccgatat caggtacgac aacttgtacg
     3241 gtagcgccac cggcaggagc ggctgcaggc gcagtagctg gagcagcagg agcttctgct
     3301 gcaggggccg gagcggcatc agcagcggca gcaccggttt caacggtcag aataacgccg
     3361 ccttcagaga ttttatcgcc aactttaact ttaacttctt taacaacacc ggctgcatca
     3421 gcaggtacgt ccatagtggc tttgtcggtt tccaaagtaa tcagggtgtc gtcaacggca
     3481 atagtgtcgc ctactttgac ttctacggcg atgatgtcga cgttttcatg accgccgata
     3541 tcggggactt tgatttctac gatactcatt tgagttcctt taatcttttt caattggatg
     3601 ccgtctgaaa gctgcttcag gcggcatcgg tacggtttgt ctgctcaaac cattcgggca
     3661 ttcggttttc agacggcctg atgtcggaat ggggcaaaca ggccgtctga aacatcaatc
     3721 agcgtttcca gctaggagct gaatcggctt tgatgccgta tttctcaatg gcttgttgaa
     3781 cggtttcttt gctgactttg ccttgttccg ccaatgcggc caatgcggcc acggcaacgt
     3841 tgtagcgatc cacttcaaag aagcggcgca ggttggcgcg actgtcggaa cggccgaaac
     3901 cgtcagtgcc caagacgtgg tagtcgttcg ggatgtaggc gcggatacgg tcagcatagc
     3961 tgcggatata gtcggtagcg gcaatcaccg gaccgtcatg accttgcagt tgggaagtaa
     4021 cgaaaggtac tttttcggct tccagcggat gcaggcggtt gaagcgttct acctcgacag
     4081 cgtcgcggtg cagcaggttg aaggacgggc aagaccagat gtctgcttct acgccgaagt
     4141 cggctttcag cagctcggca ccggcaatga cttcttgcag gatggtaccg gagcccatca
     4201 attgaacttt cttgtcgcct ttgccgccgg ctttcagcag atacatacct ttcaggatgt
     4261 cttgttccgc accttcgggc atatccggat gggtgtagtt ctcgttcatc agggtgatgt
     4321 agtagaacac gtcttcatta ttggcataca tacggcgcag accgtcttgt acgatgacgg
     4381 cgacttcgta ttggaaagtc gggtcataag atacgcagtt cggaatcagg tcggcctgga
     4441 tgtggctgtg gccgtcttcg tgttgcaggc cttcgccgtt cagcgtcgta cggccggcag
     4501 taccgcccag caggaagccg cgcgcgtgca tatcaccggc tgcccatgcc aagtcgccga
     4561 cacgttggaa accgaacata gaatagtaaa tgtagaacgg aatcatcgcg aagttgctgt
     4621 tggcgtagct ggttgcagca gcgatccagt cagccattgc gcccggttcg ttaatacctt
     4681 cttgcaagat ttgaccgtca acggattctt tatagaacat cagttggtct ttgtcttgag
     4741 gggtatattg ctgacctttc ggattccaaa taccgtattg gcggaacatg ccttccatac
     4801 cgaaagtacg gctttcgtcg ggaacgatag gtacgacgcg tttgccgatt tttttgtctt
     4861 tcaacaaggt tgacaaaatg cggacaaatg ccatagtggt tgagaattcg cggtcgccgc
     4921 tggatttcag ttgggtatca aatgcggaca gctccggcac ttccaaaact tcttgggtag
     4981 gtttgcgttg cggcaggtag ccgcccaaag catcgcggcg tgcgtgcagg tatttgtatt
     5041 cttccgtatc gggggcaaaa gtcaggtaag gcagatcgcc gctttcgatt tgttcgtcgg
     5101 taaccggaat gtcaaagcgg tcgcggaatt gtttcaggga cgctttgtcc atttttttgg
     5161 cttggtgggc aacgttctga ccttcgccgg atgcgcccat accgtaacct ttaatggttt
     5221 tcgccaagat gacggtaggt ttgccgtcgg catggttcgc tgcgcggtcg taggcgttgt
     5281 acactttttg cgggtcgtgg ccgccgcggt tcaatgccca gagttgctca tcggtcatat
     5341 cggcaaccaa tgctttcagt tcgggcgtat tgaagaagtg ttcgcgcaca tacgcgccgt
     5401 ctttggattt gtaagtttgg tagtcgccgt ccaaacattc ttccatacgt tggcgcagga
     5461 taccgtcttt gtctttcgcc aagaggcggt cccagcggcg gccccaaatg actttgacga
     5521 cattccagcc ggcgccggca aagttgcctt ccaattcttg gatgattttg ccgttgccgc
     5581 gtaccggacc gtccaagcgt tgcagattgc agttgatgac gaaaatcagg ttgtccaagc
     5641 cttcgcgtgc agccagtgcg attgcacctt gagattcggg ttcgtccatt tcgccgtcgc
     5701 cgcagaaaca ccatacttta cggcctttgg tttttgccaa accacgcgat tccaagtatt
     5761 tcaggaaacg cgcctgataa atcgccatga tgggccccaa gcccatggat acggtcggga
     5821 actgccaaaa gtcgggcaag aggtgggggt gcggatagga aggcagaccg tgtccgtcca
     5881 cttcttggcg gaagttgttc agctggtctt cggtcagacg gccctcgacg aatgcgcgtg
     5941 catagatgcc cggggcgacg tgaccttgga agaagaccaa atcgccttct tcgccttcgc
     6001 ctttggcttt ccaaaagtgg ttgaaaccga cttcgtacat ggtggcggca gattggaaag
     6061 atgcgatgtg cccacccagt tccaaatctt tcttgccggc gcgcaatacg atggcggcgg
     6121 cgttccagcg cacgaatgcg cggatgcggt gttcgatgtt ttggtcgccc ggaatgcctt
     6181 tttcgttttc aaccgaaacg gtattcaaat acggggtggt cgtgccgtgt ggcatacgta
     6241 cgcccttgtc gcggcagtat ttgaccaggt tttccaagag gtattgcgcg cgttcgccgc
     6301 cttcatattc gaggacggag cttaacgcgt ccagccactc ttgggtttcg atagggtcaa
     6361 catcgtgtaa ttgggtggac atagtatcta tcctttatgt tgagtgttta atcggcaggg
     6421 agggtgtagc caaccccttt attttcgttt gtgaaaaata ataagcaaaa acgaaaattt
     6481 ctaaatcaaa ccgaaaaaaa taatttaagt ttaatgtttt caaaaaatta taaaagtttt
     6541 ggaaacaagg gaaaacttga aaaaaatgtt cgatctgctc cacacgaaat cctatcagca
     6601 atcgggtctg ctggcaaata accatgcctg taaaagcagg ttttatactg aagttggcgt
     6661 ttggtaaggt caagtctttt tgatttcgaa atattctgtt tgattttggc gatatttttt
     6721 cggaattgtg gtatatcgaa tcattatgtc tctgtcggca attttcctat tgtagaaaat
     6781 ctatacacaa gctttgtgga taaccgtgtt gataagccac ggcttgtgtt aaaaaagtct
     6841 tacgcaacaa atgtacgctt attttgatta aaaaataggc acatttaaat ctataaaaat
     6901 catgtgctta tgttcaatat gatgtttctg gtcatgtttt tgccgtctgt tatgggattt
     6961 ctcccgcgct tggggcaggg ggtgaatttt ggaggttggt gcaaaacctg cttttttcgc
     7021 gttacaatct gcacccgatt tacttttgaa tttgcctgtt tggaaaaata cttttatgaa
     7081 agccagccaa ttctttatct ctactttaaa agaagcccct gccgaagccg cgcttgccag
     7141 ccacaagctg atgattcgcg ccggtctgat taaagccaac gcgtccggcc tttatacttg
     7201 gatgccgatg gggctgcgcg tgttacgcaa agtcgaaaac gtcgtgcgcg aggaaatggc
     7261 gcgcgcgggc agcgtggagc tgctgatgcc ggtggtgcag cctgccgagc tgtggcagga
     7321 atccggccgc tgggaatttt acggtaaaga actgctgcgc ctgaaagacc gccacgaccg
     7381 cgatttctgc atgggcccga cctgcgagga agtcatcgcc gacatcgtgc gtaaagagat
     7441 caacagctac aaacaactgc cgaaaaactt ttaccacatc caaaccaaat tccgcgacga
     7501 agtgcgcccg cgtttcggcg tgatgcgcgc gcgcgagttc gtcatgaaag acgcttattc
     7561 cttccatgcc gactacgcct cgcttcagac gacctatgat gccatgtatg acgcttactg
     7621 ccgcatcttc acccgtctgg gcttggcatt ccgccccgtc gccgcagaca ccggcagcat
     7681 cggcggtacg ggttcgcacg agtttcaagt gttggcggaa agcggcgaag atgttattgc
     7741 gtacagcgac acttccgatt acgccgccaa tatcgagttg gcaccgacct tgccgcttaa
     7801 aggcgaacgc gccgctgctc aggctgtgtt gaccaaagta catacaccaa acgtcaaaac
     7861 cattgagtct ttggttgaat tcctgaatat tccggttgaa caaaccctca aatccatcgt
     7921 ggtcgaaggc gaaaacgaag gcgaaatcgt cttattactg ttgcgcggcg accatgagtt
     7981 caacgacatc aaggcagaaa aactcgcagg cgtgagatcg ccattgacga tggcaagtcc
     8041 tgccgcgatt gtcgaacagt tcggcgcaaa cggcggctcg ctgggtccgg tcagctttgc
     8101 aggtaaagtt tatgccgatt ttgctactga aaaaggagcg gactgggtca tcggtgcgaa
     8161 cgaagacggc taccactata ccggcttcaa cttcggtcgc gatgccgccg agcctgaatt
     8221 cgtcgatttg cgtaatgtgg tcgaaggcga cgaaagcccc gacgggcaag gccgtctgaa
     8281 actggcgcgc ggcatcgaag tcggacacgt cttccagttg cgcgacaaat acacccaagc
     8341 catgaacgtg agcttcctcg acaacaacgg caaatcgcaa atcatggaaa tgggctgcta
     8401 cggtatcggc atcacccgcg tcgttgccgc cgccatcgag cagaacaacg acgaaaaagg
     8461 catcatctgg acgaaggcga tggcgccgtt tgaagtcgtt atcgtgccga tgaactacaa
     8521 aaaatcagac gccgtgcgcg aagccgccga caaaatctac gccgaactgc tggcggcagg
     8581 cgcagacgtc ctcctcgacg accgcgacga acgcgcaggc gtgttgctga acgattccga
     8641 gctgttgggt attccgcacc gcatcgtcat cggcgaccgc gctttgaaag aaggcaatgt
     8701 cgaatacgcc gaacgccgca acaacgaagc gcaggcagtt gcaatcggag aaattgttgc
     8761 gcgtgtaaca gcttcattaa atgggtaagc ttggtcaaat gctgtctgaa aataatttca
     8821 gacggcattt ttatttgggg agaaagtgaa gtggcttcag tatttttaga aggagaagca
     8881 gtcgaggtcg gcaatatttt ctgtatcggc aggaactatg ccgcacatat tgaagagtta
     8941 aaaaacgaaa taccttccga acctgtggtg tttatgaagc cgtcaggcag cattttgaat
     9001 agcggcggta caatcttgct gcccgagttt agtagggatg tgcagtttga gtgcgaactc
     9061 gtgttgctgg tcggtaagga ttcagacggc atgggagagg gtgaagatat tttgggatgt
     9121 gtggcggggt acggcgtggg tttggatttg accgcgcgag acatccaatg ccatctgaag
     9181 gcgcaggggc tgccctggct gaaggcaaaa gggttcaggc attccgcgtg cgtgtcggat
     9241 tttgcggcgg cagacagaat cggcaatccc gagaaggtgt tgttttcatt aaaacaaaac
     9301 ggcgtattga aacagcgcgg ggataccggt ttgatgattt acccgattcg ggaaatttta
     9361 cacaaactgg cggcggatta cggtttgggg aaaggggatt tggtgtttac cggaacgccc
     9421 tccggcgtgg gcgcaatcgg tgcgggggac aatttggcat tggagctgga cggcttggtg
     9481 cgcgcctctt tcacggtagg gtgttaaccg cgccgcttaa ccctcacgcc cgttgaaaca
     9541 ttccgccgga ccgccctcga aaaaaccgtg caagatggct tgcgccgcca cttggtcgag
     9601 caccgatttg cgttttttgc cgaagacctg tgcttccgaa agcaggcttt cggcatagac
     9661 ggacgacagc cgttcgtcaa cccaatagac ggggagattg aacctgccgt tcagcctgcg
     9721 tccgaacttg cgcgacaggt gcgtcatttc atgtttcgtg ccgtcggtat gcacgggcag
     9781 tccgacgaca aaataacgcg gctgccattc ttgaaccagc ttggcgattg ccgcgaactt
     9841 ttcatcgttg ctgccgccgg taacggtgct caaaggatgg gatagcccta attccgcgtc
     9901 tccttgtgcc acgccgatac gcgcttcgcc gaagtcgaat gccagtgccg ttccttttgg
     9961 aattttatgc atggccggct cccgaaaaca gggcgagcgg gtcgatgccg agtttggcga
    10021 atgcggcggc gtaacggtgt tcgtagggga tgtcgaacag gatgtgttcg tcggcgggaa
    10081 cagtcagcca cgcattgtcg gcaagttcgc gttcgagctg gccttttccc cagcttgaat
    10141 agcctatgct gatcaaggct ttgtcaaccg caccttcgcg tgaaatattt tcaatcacgt
    10201 ctcgggaaga agttagcgcg atattgtctg aaacgccgat actgctttgc cagttgccga
    10261 tcggggtatg cacgacataa cctcgctcga cttgcaccgg cccgcccatc atcacgctgt
    10321 cgtgctgcat ccgcatgggg atgtttttgc cggtggcgga aaaaatcatg tccatcgtaa
    10381 tcggagaggg tttgttgatg gcgatgccga gtgcgccgtc ttcatcgtgt ttgcagatat
    10441 agacgaccga ttgtgaaaaa aacgcgtctt ccatatcggg catggcgacg agaaagtggt
    10501 tggagaggtt catatcagcg tttgtcggga ttttctatcg ggttggaaat catgcaggtg
    10561 ccggcaaaca gtttgccgga agtatcggct tgcaccccat cggtttgcgg tatgccgccg
    10621 ccgaaacagg aaaccgcgct taaggaattt ttcggcgagc cttggatgat tttatcgctg
    10681 tacaccaaat aggcgaaggt tttgcgtttg gggtcgtaat aacggacaat ctgccggctc
    10741 ttgaacgcga agctcgcacc gtgtttgaaa acttctttcg gtttgcgcac ggcggtttcg
    10801 tcaaaagaaa tcgaagatgc cgtctgaacg cacgaaaccg atgcgtcgga cgcgtcctct
    10861 tccaaattga ccatttcctt caagccgcct ttttttgcat acgaaatata acaggcaacc
    10921 ccttgaacgt cgggatcgtc gaatccttcc acttcgatac ggtcgttttt gcccagcatg
    10981 ttgaaaacgg tacttgcccg tccgatttta tcggtttcgc cgctgccgca ggcagtcagc
    11041 aggacggcgg cagacagcag tagcagtctg ttcatcaaaa tttccttatc ggttgaatcc
    11101 ccgttttccg tgaaggcggc agggcttgct ttgtttggag gggcgcacgt tcccaaaatc
    11161 gggcaacata cggtatgcgg cttaatgtgc taccctgtgt gtcggaacac aatatccgtt
    11221 gcggcattga aaatatgacg cacaatataa ggctgtttga gccgattgca agtatccgcg
    11281 caaagaaggc gcaagattgg gcaaacggca ttttcagacg gcataactga cagtataatc
    11341 caaacgtccg gctgctttcg tgcagccttc agtattatcc aactttttcc gaaggtcgaa
    11401 ccatgcgcta caaacccctt ctgcttgccc tgatgctcgt tttttccacg cccgccgttg
    11461 ccgcccacga cgcggcacac aaccgttccg ccgaagtgaa aaaacagacg aagaacaaaa
    11521 aagaacagcc cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatggc gcagtgaaag
    11581 ataaaaaaac aggcggcaaa gaggcggcaa aagagggcaa agagtccaaa aaaaccgcca
    11641 aaaaccgcaa agaagcagag aaggaggcga catccaggca gtctgcgcgc aaaggacgcg
    11701 aaggggataa gaaatcgaag gcggaacaca aaaaggcaca tggcaagtcc gtgtccggat
    11761 ccaaagaaaa aaacgcaaaa acacagcctg aaaacaagca aggcaaaaaa gaggcaaaag
    11821 gacagggcaa tccgcgcaag ggcggcaagg cggaaaaaga cactgtttct gcaaataaaa
    11881 aagtccgttc cgacaagaac ggcaaagcag tgaaacagga caaaaaatac agggaagaga
    11941 aaaatgccaa aaccgattcc gacgaattga aagccgccgt tgccgctgcc accaatgatg
    12001 tcgaaaacaa aaaagccctg ctcaaacaaa gcgaaggaat gctgcttcat gtcagcaatt
    12061 ccctcaaaca gcttcaggaa gagcgtatcc gccaagaacg tatccgccaa gagcgtatcc
    12121 gtcaggcgcg cggcaacctt gcttccgtca accgcaaaca gcgcgaggct tgggacaagt
    12181 tccaaaaact caataccgag ctgaaccgtt tgaaaacgga agtcgccgct acgaaagcgc
    12241 agatttcccg tttcgtatcg gggaactata aaaacagcca gccgaatgcg gttgccctgt
    12301 tcctgaaaaa cgccgaaccg ggtcagaaaa accgcttttt gcgttatacg cgttatgtaa
    12361 acgcctccaa tcgggaagtt gtcaaggatt tggaaaaaca gcagaaggct ttggcggtac
    12421 aagagcagaa aatcaacaat gagcttgccc gtttgaagaa aattcaggca aacgtgcaat
    12481 ccctgctgaa aaaacagggt gtaaccgatg cggcggaaca gacggaaagc cgcagacaga
    12541 atgccaaaat cgccaaagat gcccgaaaac tgctggaaca gaaagggaac gagcagcagc
    12601 tgaacaagct cttgagcaat ttggagaaga aaaaggccga acaccgcatt caggatgcgg
    12661 aagcaaaaag aaaattggct gaagccagac tggcggcagc cgaaaaagcc agaaaagaag
    12721 cggcgcagca gaaggctgaa gcacgacgtg cggaaatgtc caacctgacc gccgaagaca
    12781 ggaacatcca agcgccttcg gttatgggta tcggcagtgc cgacggtttc agccgcatgc
    12841 agggacgttt gaaaaaaccg gttgacggtg tgccgaccgg acttttcggg cagaaccgga
    12901 gcggcggcga tgtttggaaa ggcgtgttct attccactgc accggcaacg gttgaaagca
    12961 ttgcgccggg aacggtaagc tatgcggacg agttggacgg ctacggcaaa gtggtcgtgg
    13021 tcgatcacgg cgagaactac atcagcatct atgccggttt gagtgaaatt tccgtcggca
    13081 agggttatat ggtcgcggca ggaagcaaaa tcggctcgag cgggtcgctg ccggacgggg
    13141 aagaggggct ttacctgcaa atacgttatc aaggtcaggt attgaaccct tcgagctgga
    13201 tacgttgatc ggattgccga aacggtaggc tttatgcaaa tcgtgccgat atggtgcgct
    13261 gttccgtgaa gtttgccgtt tgccgtacct gtattttccg gtttttggaa atcaagagag
    13321 aaagttaaag aagaatgtcg aaacctgttt ttaagaaaat cgcactttat actttgggtg
    13381 caatcagcgg cgtggccgtc agtctggcgg tgcagggttt tgccgccgag aaggaccggc
    13441 gggataacga agtcctgccg gtgcaatcca tccgcacaat ggcggaggtt tacggtcaaa
    13501 tcaaggcaaa ctactatcag gacaaacccg atgccgattt gtttgaaggt gcgatgaagg
    13561 gtatggtggc cggtttggat ccgcattccg aatatatgga taaaaaaggt tatgccgaga
    13621 taaaggagtc caccagcggc gaatttggcg gcttggggat ggaaatcggg caggaagacg
    13681 gatttgtcaa agtggtttcg ccgattgagg acacgcctgc ggaacgggcg ggggtgaaaa
    13741 gcggcgattt cattgtgaaa atcgataatg tttcgacacg cggcatgacg gtcagcgaag
    13801 cggtgaagaa aatgcggggc aagccgggta cgaagattac tttgacgctg tcgcgtaaaa
    13861 atgccgacaa gccgatagtc gtcaacctga cgcgcgccat cattaaagtg aaaagcgtcc
    13921 gccatcacct gattgaaccc gattacggct atatccgcgt gtcgcagttc caagagaaga
    13981 cggtcgaaag cgtcaataca gccgcaaaag agctggtaaa ggaaaataaa ggaaaaccgc
    14041 tcaaggggct ggtgttggat ttgcgcgacg accctggcgg gcttttgacc ggcgcggtcg
    14101 gcgtgtcggc ggcgtttctg ccgtctgaag cggtcgtcgt cagcaccaag ggacgcgacg
    14161 gcaaagacgg catggtactg aaagccgttc ccgaggatta tgtgtacggt atgagcggcg
    14221 atcctttggc gggcattcct gccgagttga aaaccatacc gatgacggta ttggtcaatt
    14281 ccggttcggc ttccgcgtcg gagattgtcg caggtgcatt gcaggatcat aaacgcgcgg
    14341 ttatcgtcgg tacgcagagc ttcggcaaag gttcggttca gactttgatt cctttgtcca
    14401 acggcagcgc ggtcaagttg acgaccgccc tgtattatac gccgaacgac cgttctattc
    14461 aggcgcaggg gattgttccc gatgtcgaag taaaagataa ggaacgcatt tttgaaagcc
    14521 gcgaggcgga tttggtcgga cacatcggca atcctttggg cggcgaggat gtgaacagtg
    14581 aaacccttgc cgtgccgctt gaaaaagatg cggataagcc cgctgtaaaa gaaaaaggta
    14641 aaaagaaaaa ggacgaggat ttgtcttcaa ggcggattcc caaccctgcc aaagacgacc
    14701 agttgcggaa agctttggat ttggtcaagt cgcccgagca gtggcagaag tctttggggc
    14761 tggcggcgaa aaagccggtt tcaaataaag ataagaaaga taaaaaagat aagaagtagg
    14821 tttttacgat gccgtctgaa aggatttcag acggcatatt atgtttgaaa ggacaggtta
    14881 tgagcgattt tttaccggaa tttgaaccgt gcggcattta tagtgggtta acaaaaatca
    14941 ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg caacgccgta
    15001 ctggtttttg ttaatccact atacttgggt agcgattggg tttcagacgg catacattca
    15061 ggatttcacc gccttatgcc gtctgaaaga cgtttccgac ccatccctac tacacgcttt
    15121 gtccgcaaac gcatcacagg caagattatg gaagtcattc gataccccga ttcccccttc
    15181 aaactccacc aacccttcca gcccgcaggc gaccagccta ccgctattgc cggcttgctc
    15241 gaagggcttt cagacggcct tgcctaccaa accctgctcg gcgtaaccgg ttcgggcaaa
    15301 acctacacca tggcgaacgt catcgcccaa agcggccgac ccgccatcat catggcgcac
    15361 aacaaaaccc ttgccgccca gctttatgcc gaaatgcgcg agtttttccc tgaaaacgcg
    15421 gtggaatatt tcgtctccta ctacgactat taccaacccg aagcctatgt gcccagccgc
    15481 gacctgttca ttgaaaaaga cagcgcgatc aacgaacata tcgagcagat gcgcctttcc
    15541 gccaccaaga acctgatgac gcgcgacgac gtgattatcg tcgccaccgt gtccgccatc
    15601 tacggtatcg gcgacccgac cgagtatcag caaatggtgt tgtccgttaa agaaggcgac
    15661 accattgagc agcgcgacat catcgccacg ctcgtttcca tgcaatacga acgcggcgat
    15721 ttggacttta aacgcggcag cttccgcgtg cgtggcgacg tgattgacgt gtaccccgcc
    15781 gaaagctccg aaaacgcctt gcgcatcagc ctgtttgacg acgaaatcga ccgcctcgat
    15841 atgttcgacc cgctttcagg cagccttatc cagcgcgtcg gccgctacac cgtcttcccg
    15901 tccagccatt acgttacccc gcgcgatacc gtcttgcgcg cctgcgagtc catcaaagaa
    15961 gaattacgtg aacgcattga tttctttacc aaagaaaacc gtcccgtcga acaacaacgc
    16021 atcgaacagc gcacccgctt tgacctcgaa atgctctacg aaatgggctt ctgcaaaggc
    16081 atcgaaaatt actcccggca cttctccggc aaaaaagaag gcgaaccgcc gcccacgctg
    16141 atggactacc tgcctgacaa cgccatcatg ttcatcgacg aaagccacgt taccgtcacc
    16201 caaatcggcg gcatgtacaa aggcgacgca tcgcgcaagc aaaacctcgt cgactacggc
    16261 ttccgcctgc cttccgcccg cgacaaccgc ccgctcaaat tccatgaatt tgaaaaagtc
    16321 atgccgcaaa ctgtcttcgt ttccgccact ccagccaaat acgaagaaga acacgccgga
    16381 caagtggtcg aacaagtcgt ccgccccaca gggctggtcg acccccaaat catcatccgc
    16441 cccgtcgcca cccaagtcga cgacttaatg agcgaaatca acgaccgcat ccaaaaaggc
    16501 gaacgcgttc tcgttaccac cctcaccaaa cgcatggcgg agcaactcac cgactattac
    16561 agcgaactcg gcatcaaagt gcgctacctg cacagcgaca tcgataccgt cgagcgcgtt
    16621 gaaatcatta gagatttacg gctcggcctg tttgacgtac tcgtcggcat caacctcttg
    16681 cgcgaaggcc tcgatatccc cgaagtctcc ctcgtcgcca tcctcgacgc cgacaaagaa
    16741 ggcttcctgc gctcacaccg cagcctgatt caaaccatag gccgcgccgc gcgcaacgtg
    16801 aacggcgtcg cgattctgta cgccgacaaa atcaccgact ctatgaaagc cgccatcgac
    16861 gaaaccgaac gccgccgtga aaaacaaatt aaattcaacg aagaacacgg catcgtgccg
    16921 caacagatta aaaaacaggt caaagacatc attgacggcg tgtaccacga agaagacagc
    16981 ggcaaaggcc gtagacaagg caaaaacaaa gttaaagtcg gcgaaattca caacgaagaa
    17041 gacgcgatta aagaaatcgc caaattggaa aaagccatgc aacaggcagc tagggatttg
    17101 cagtttgaag aagcgtcggt gttaagggat aggattcggg ggattaggga agggttgtta
    17161 tttgggagtg agtaataaaa gcacttgaaa gtttagttga tgaccacaat gttcttaaaa
    17221 gatttggaga aacaaaagat gtggagaaac atcgtaactt gatcaattac attctgagga
    17281 ctgaaaatga catattcttt tcgtgtagaa aacataaatg atatatctga tattcctgaa
    17341 aatcatttgg aaatagttag agctgctctg cctagtaata gaagtaatgc cttatctcgt
    17401 gagccagatg gggcacaagc tgtttttgtt tatgaaattg atggtgaatt tagagctaag
    17461 ttatttgacc gcataaaaga tacagacagt tatttacaag caacagagga taattactct
    17521 aatgaccctc gcagtataga agtagtatac gttttggagc aacagcaagg gtagcctgag
    17581 ggtcgcacaa actctcaagc tgacgtgcag tatgtgtgat acgcacgcat gcgttttttc
    17641 gttcaaacca ttgttttaaa ccttgtatcg aaacatcact cgctgcttta tcttgaaaaa
    17701 atgccgtctg taagcaaaca tgcttcagac ggcatttttt caaacagcgg tttgttatct
    17761 gatccgtttg aataccaagt cccaaacgcc gtgtccgaga cgtttgccgc gcgcttcgaa
    17821 tttggtttcg gggcggtagt ccggggtagg ggcgtagtct gtcgccgtat tttgcaggct
    17881 gtcgaagcta ctgaggactt caagcatctg ctgtgcatat tcttcccagt ctgtcgccag
    17941 gtggatatag ccgccggttt tgagtttggg cagtagtttg gcgatgaagg gggcttgtat
    18001 cagacggcgt ttgtggtggc gttttttgtg ccacgggtcg gggaagaata tgtggatgcc
    18061 gtcgagcgag ccgtcttgca gcatattttc gacaacttct acggcatcgt gccgcatcac
    18121 gcggatattt tccaaatggt tttcttctat gagtttgagc aggttgccta cgccgggacc
    18181 gtgtacgtcg atggcgagaa agtcggtttc gggcaggcgg cgggcgattt ctgcggttgc
    18241 cgtccccata ccgaagccta tttcgaggat tttgggtcgg ttgcttccga aattggcatc
    18301 aagatcggcc ggtgttgctt ggtaatcgat gccgaagctg tcccataagg tatcgatggc
    18361 gcgttgctgt gcggcggtca tatggctttg gcggaggacg aagctgcgga tgctgcgggg
    18421 atgttcggct tgccagttgt tttgtgtttg gttttcggcg gtgtctgtca taatgcttcc
    18481 gtgcgcttgt gcaatcagga tgccgtctga atgtgaaagg cctgttcgga cggcgggaag
    18541 ggaaaggggc gattatacct gtttcgtttc ggcagggtag gtgtaacgct gttcagacgg
    18601 cattttgcca ctgttgcaac agttcttgca ggacgctttt ttgcccgtat ccgttgttta
    18661 tggcttcttg caggtagcgg caggcttttt ctttgtccgg cccgacaccg tgtccgctgt
    18721 aatggatgca ggcgaggttg tagaacgcca tactgtcttt ttgcgcggcg gcttgttcaa
    18781 accatttgcg ggctttgccg tagtcggcgg ctatgccctg tccgaagtaa tacatactgc
    18841 ccagattggt ttgggctttg ctgtgtccga gttctgcggc ttctgtgtag agtttgagcg
    18901 cggctgcgta ttcgggcgcg cattgtaatc cgtaatgcag ggcttgggct ttttgataaa
    18961 gctgctctgc ctgttgccgc ctgatggcgg aatctttgat gccgccgtat tggtcgggat
    19021 ggtttaacgc gctgtcggat atgagtttct gatacgcggc aaggctgccg gcttcggctg
    19081 cctgccggta ataatgccgg gctttttctt tatcggaagc gatgcccagc ccgtagcggc
    19141 agatgtcgcc cagtatccgc aaaccttcgg gatggcgttc ggcggcggct gcttcggcgt
    19201 gatgcagggc aagtttgtgg tttctttcca gatacttgcc ttgcaggtag atgtcggcca
    19261 gtgcggcatg ggcatcgggg tcattttcct gtgccgcctg ttggaaccgg taaacggcct
    19321 catcgggagc cgtaggtagg atgagttcgg caagcggggt gcaggcggga atgtatcctt
    19381 gttgcgcggc ggcacgcaaa tgattgacgg cctgtgccgt gtcggtttgc gtcccttgtc
    19441 cgaaacgata ttgcagcccg agctgccatt gtgcttccgc caagccttgt accgccgctt
    19501 tgcggtagaa caggtgcgcc gtttcaaaat ccggcggtgt gctgtaccgg cagtattggg
    19561 cgagcaggta ttgcccgtgg ggattgttgt tttttgcggt cgtttttgcc cattcgagtg
    19621 cttcttcaaa atgccgggac gcgtacagca ggtacatcag gcggacttgc gcttcggaca
    19681 gacctgcttc tgccagaggc ctgtaaagcg gtatcagggt ttcagggggc gcgccttcca
    19741 attcttggat atatgccaga ttgtaacgcg cgtagggatg cccctgttcg gcggcgatgc
    19801 ggagcatttc cataccttgt tcgcggtctt ggtaggggct gtccgcatcc atcaggtgtt
    19861 ttgccaactc gaatgcagcg gcgggatgac ctgccgagaa cgcttctgtc agcagtcggc
    19921 ggacttcggt aaagttatat tgtttttgag acaggcgttc taaggcctgc cgatagggta
    19981 agtcgggatt gttcatatca ggtcgttttc cgttttgtca aatcatcgtt attgtgttat
    20041 gaagttaggt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
    20101 acaaatagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc cactatatat
    20161 cgatataccg acctgctgta tcatattttt tacagacgga ttttgtcttt gacagcatgc
    20221 taatgcagat gattgtaaat ggtttccgcc aatgccttgc tgatgccttc cacttgttcc
    20281 aaatcctcgc ggctggcggc aatcacgccg cgcagaccgc cgaagcgggt aagcagggct
    20341 tggcggcgtt tgctgcctac gccggggatg tcgcttaagg aggaggtaac gcgggctttg
    20401 tcgcgttttt tgcggtggcc ggtaatggcg aaacggtgcg attcatcgcg tacggtttgc
    20461 aataaatgca aggccgggct gttgggcggc aagcggaaga cttcgccggt aaaaggcagt
    20521 atgagctcct ccataccggc tttgcgctcc gggcctttgg cgataccgac caaaggaatg
    20581 tgcagcccga gttcttccca taccgatacg gctacgccga tttgcccttt gccgccgtca
    20641 atcaatacga catcgggcca tttgacggtc tcgccgttgg cttcggcctc ctgcattttg
    20701 ccgtaacggc gcgtcaacac ttcgcgcatg gcggcgtagt cgtcgccggg tttggcggtc
    20761 gtgatgttgt agcggcggta ttgcgagggc tggatgtttt gctcatcgta cacaacgcag
    20821 gacgcaatag tggcctcgcc ttgcgtgtgg ctgatgtcga agcattcgag gcggttgagg
    20881 tcgtctgaat tcatgttcag gatttttgcc aactcgtcga tacggtgttg ctggctgctt
    20941 tgttgcaggc agcgttgtgc aatcgccatt tgcgcgtttt gttccgccat tttcaaccaa
    21001 actttgcgtt cgcctatggt tttggtaacg aactgcattt gtttgccatg ttcgccttcc
    21061 aaagcctctt ttagcgcatc gggaacggga aagttgctga tgatgatgtc gggtttgctt
    21121 ttgcctagat agtgttgggc gacaaaggct tcggcgtaat cttgtccgtt tggctcggga
    21181 tcgtttttgg tgtcggggaa aaagctttta tcgccaacgt gccgtccgcc gcggatgctg
    21241 acccagtgta cgcagacgag gccgtctgaa acggcaagcg caagcaggtc gatatcgttg
    21301 gggttgttcg ggtttttgct gtcgatgaac tgattactct gcatgatgcc gagcgcttgg
    21361 atttggtcgc ggtagcgggc ggcttcttca aattgcaaat tcgcggcggc agtttgcatt
    21421 ttgtgttgca gggtgcgggt cagttcgtcg gtcttgccgt tgagaaaagt cgaggcctcg
    21481 cgcacgctgt cgtggtagtc ttcttcgctg atatggccga cacagggtgc gctgcagcgt
    21541 ttgatttgat acagcaggca agggcggtcg cggtgttcga acacgctgtc ttcgcaggtg
    21601 cgcagcataa agactttttg caggacttgg atgctgtcgc gcacggcgtt gctgttagga
    21661 taggggccga agtattggtt cggctttttc agtgtgccac ggtaatacgc catctgcgga
    21721 tattgatgtc cgctgagcat gaggtaggga tagcttttgt cgtcgcggaa aaggatattg
    21781 tatttcggcg acaaggcttt gatgaggttg ttttcgagaa tcagggcttc ggcttcggaa
    21841 cgcgtgatgg tggtttcgat atgatgaacc tgttttacca tcaatgcgat gcgcggcgaa
    21901 tggtcgtttt tttggaaata gccggacacg cgccgcttga ggttgaccgc tttgccgacg
    21961 tataagactt tgccgccttc gtcaaaaaaa cggtacacgc ccggcagctt gggcaggttt
    22021 ttgaggaaga gcgggatatc gaattgttcc ggagaacggt tttctgtatt catatcaatt
    22081 atttgaaaaa cggcttcaga cggcatatcg gcagcggcag ccttatccgt tcggaaaagc
    22141 tgtcgggtgg ttatatgcgg tcaatcgatt tttacccgtt tcaggcgcaa ggcgttgctc
    22201 aacacggaaa ccgagcttgc cgccattgcc gcgcctgcga tgacagggtt taaaaaaccg
    22261 agcgcggcaa gcggaatgcc caaaatattg tagaagaagg cgaaaaacag gttttgcttg
    22321 atgtttctca acgtcgcccg cgataccgat agggcatcgg cgagttggtt gaccgaatgt
    22381 tgcatcagcg tggcggatgc ggtgtgttcg gcaacgtccg cgccgccttt catggcgaag
    22441 ctaacgttgg cggcggcgag cgcaggtgcg tcgttgatgc cgtctccgac catcgccacg
    22501 gttttgccgg cggctttgag tttctgcact tcggcggctt tgtcgcgcgg actcatatta
    22561 ccgaaggcgt gtgcgatgcc cagttgtttg gcgacgtact cgaccgtgcc ttggttgtcg
    22621 ccgctcataa tatagacatc gatattgtgt tttttcagac ggcctatggc ttcggcggta
    22681 tcggctttca acgcgtcggc aagtgcgaat gcgccgatgg gtttgttatc gactgagacc
    22741 gcaacaatgc ttgcaatatc ccaaacgccg tctaaaaact tcggcaaggc cagttcggca
    22801 aattcggctt tgcctgcttt caccaaaccc acgccttcca cttcggcggt aatgcctgcg
    22861 ccgacaacgg tttgtgcgtt ttgtgcggcg ggaatgtcca aaccgcgcgc ttgggcggcg
    22921 gagacgatgg cacgggcgag cggatgggcg gcgttttgtt cgacggcggc ggcgatgcgg
    22981 tacaaagcgt cttcgtcaaa gccgctgtcg ggaacgcaat aaacggcggc aacctgcggg
    23041 ctgccttcgg tcagcgtacc ggttttgtcc aacacgacgg catcgacgtg ggcggcttcc
    23101 tccattgctg ccgcgtcttt aaaccaaata ccgtgtttaa ccgctttgcc cataccgacc
    23161 ataatcgcgg caggggttgc caaaccgagt gcacacgggc aggcaatcac caaaacggcg
    23221 acggcgtgca tcagcgcaac cgtccaatcg cccttaatca gccaagtaac aataaaagtc
    23281 aacaacgcaa tgcccacgac ggcaggcacg aataccgcag ccgctttatc ggctacgcgc
    23341 gcaatcggtg ctttactgcc ttgtgcttca gagagcgcgt tcatcatgtc gccgagctgg
    23401 gtttggctgc cgagctgcgt ggcgcggtac accacactgc cttcggtcat taacgcgccc
    23461 gccaacactt tgccgcccgc ctttttttct tcaggattgg attcgccggt aagatggctc
    23521 tcgtccgccc aaccgctgcc gctttcaatg atgccgtctg cggcaatgcg ttcgccgtgg
    23581 ttggcgcgga taaggtcgcc gatttgcact tggtcgatgg gaagctgttt ccattcgccg
    23641 ttgcgttgca cgttgacttg ggttggtgta agtttgagca gcaagcccaa gctgttgagg
    23701 ctggattttt tggtacggtg ttccaaaaat ttacccagtg acacaaaacc gatcaccatc
    23761 acgcccactt caaaatacac atgcgccata ccgtacgccg cgtgcgggct gaaaaacagc
    23821 atatagacgg aatacaggta aatcgagacc gtgccgatgg taaccagcac gtccatattc
    23881 gccagtccgc ccttaatgct cgcccacgcg cttttgtaaa acgggattgc cagccaaagc
    23941 tgcaccacgc ttgccaatgc gaactgccac aacggcggaa tcatccaatc gtgtctgccg
    24001 atcatcatcc ccgccatgcc gataaggaac gggacgttga tggtgaacag cagccacagc
    24061 cgccagccga tatggtgttc tgcttcgggt tgcggcaatg tatcttccgt tttttccttc
    24121 gcgccgtaac cggttttctc aatgattttg gcaatgtcgg ctactgaggt tttgctgtcg
    24181 tcaaacacta cctgcgcctc ttcgctggcg aagtttaccc ccgccgattc gacaaaatct
    24241 tttttgttca acactttttc aatacgcgaa gcgcaggcct gacaggtcat gccttcgatt
    24301 tggaaacgga ttttttgttg cataagcgtg attcctgctt tgatgttcag acggcagtca
    24361 aggttgcggg atgccgtctg aaacgccggt atggtcaaaa tttctcaaac gcgccgataa
    24421 ttcgagtaga atccgaaaac ttgacacagc aaaggaaaaa caatgtccca acaccgcaaa
    24481 ctgattattt tgggttccgg ccccgccgga tacaccgccg ccgtctatgc cgcgcgcgcc
    24541 aatttaaacc ccgtcattat tacaggtatc gcgcaaggcg ggcaactgat gaccaccacc
    24601 gaggtggaca actggcctgc cgatgccgac ggcgtgcaag gacccgaact gatggcgagg
    24661 tttctcgccc acgccgaacg tttcgggaca gaaatcattt ttgaccaaat caacgccgtc
    24721 gatttgcaaa aacgcccgtt cacactcaaa ggcgatatgg gcgagtacac ttgcgatgcc
    24781 ctgattgtcg caacaggcgc gtccgccaaa tacctcggtt tgccgagtga ggaagcgttt
    24841 gccggaaaag gagtttccgc ttgtgccacc tgcgacggtt tcttctacaa aaaccaagat
    24901 gttgccgtag tcggcggcgg caatacggca gttgaggaag cactctacct tgccaatatc
    24961 gccaaaaccg ttacgctgat tcaccgccgc agcgagttcc gtgccgaaaa aatcatgatc
    25021 gacaaactga tgaaacgcgt ggaagagggc aaaatcatcc tcaagctgga aagcaacctg
    25081 caagaagtct tgggcgacga tcggggcgta aacggcgcat tattaaaaaa caacgatggt
    25141 tctgagcaac aaattgccgt cagcggcatc tttatcgcca tcgggcacaa gccgaatacc
    25201 gatattttca aagggcagtt ggaaacggac gaagccggtt acctgaaaac caaaggcggc
    25261 acggcggaca atgtcggcgc aaccaatatc gaaggcgtat gggcggcggg cgacgtaaaa
    25321 gaccatacct accgtcaggc aattaccagc gcggcttccg gctgtcaggc ggctttggac
    25381 gcggaacgct ggctgggcag ccaaaacatt tgaatgccgt ctgaaaccga aattttcggc
    25441 ggaatgcaag tttttcgcac caaatccttg attccgtcgg caaaccccaa tataatgcct
    25501 gctctttgaa ccttgccttc tgctttcgca tggcagaagg ctgtttttac catccataag
    25561 gagataacat gcgtcattac gagatcgtgt ttatcgttca tcctgatcaa agcgagcaag
    25621 tgcccgctat ggttgaacgt tacaaaacca tgattgctga agcaaacggt aagattcacc
    25681 gtttggaaga ttggggtcgc cgccaactgg cttacccgat taacaaaatc cataaagccc
    25741 attacgtttt gatgaacatc gaaaccactc ccgaagtggt tgaagagctg gaaaccgcat
    25801 tccgcttcaa tgatgccgta ttgcgccatc taaccatcaa aaccaaacac gccgttaccg
    25861 aagcatcccc tatgttgggt ggtgaaaagg ctaagaacct gttgagcggt gcgtctgaag
    25921 aagcggtcgc ccaataattg ggattcaata atcttgtttc gcttgccgcg ttaattgaaa
    25981 aggttttccc tattcgatat acgcctgccg gaatccctgt tttagatatt attttaaagc
    26041 acaaatcgtg gcaggaggaa aacgggcagc aatgccttgt ccaattggaa attccggcac
    26101 ggattttagg caggcaggcg gaagagtggc agtatcggca aggtgtatat gttcacgtcg
    26161 aaggtttttt agctcaaaaa agcagacgtt cccttatgcc gatgctcagg atacaaaata
    26221 ttcaagaata taaaggttaa acgacaatgg ctcgtcaatc attcaaacgt agaaaattct
    26281 gccgtttcac ggctgaaaaa atccaagaag tcgattacaa acaagttgat ttgctgaaag
    26341 actttatctc tgaaaacggt aaaatcattc ctgcacgcat cacaggaacg aaggcattct
    26401 accaacgcca attggctgtt gccgtaaaac gcgcacgctt cctggctctg ctgccttaca
    26461 ccgaccaaca caaataattt tggagattga atcatgcaaa ttattctgtt agaaaaaatc
    26521 ggcggtctgg gcaacttggg cgacatcgta accgttaaaa acggctacgc ccgcaacttt
    26581 ctaattcccg caggtaaggc aaaacgtgcg accgaagcga atatgaaaga gtttgaagca
    26641 cgccgcgcag aactggaagc caaacaggtc gaaattttgg cagatgcccg agtccgtcag
    26701 gaaaaattgg acggtcaaac cattaccgtt gcacaaaaag ccggtgtgga cggtcgcctg
    26761 ttcggttccg tcaccaatgc cgacattgct gctgcaatcg ttgctgccgg catcgaagcc
    26821 gtgaaagcaa atgtacgtct gccgaacggt cctctgaaag ccgttggcga gtacgaagtg
    26881 gaagtggctt tgcacactga tgccgttgct aaaattaccg ttgccgtcgt tgccgcaacc
    26941 gagtaattga aattatcaga tgccgtctga aaccctgccg attatcggaa tgtggtttca
    27001 gacggtattt gttttgtttt ccgtatattc tgcaaaaatc gggatggttg tttttatagt
    27061 ggattaaatt taaaccagta cagcgttgcc tcgccttgcc gtgctatctg tactgtctgc
    27121 ggcttcgtcg ccttgtcctg atttttgtta atccactata tttgcctgtg gtctttgcag
    27181 gaatagtttg ttaacgaaat ttgatgcata aaatgcgcca aaaaatattg gctgtgtttt
    27241 aatatagtgt tgataagcgt acctggcttc aatatactca ataggatagg tctcttccta
    27301 accgaaaaaa taattgtctt ttcccttgca aataattgaa atcaacagat tactaaaaca
    27361 cagccaaaat attcaattga caaaaacttt tctgattttg agaaatctgt gaaaatcagg
    27421 ggattattta ttttgaaaat ggtatcgggc ataaaatttg gctgtgtttc aatatggtgc
    27481 tgataacata ctttgtttca aaatgttcaa taggataact tcctgattga ccgaaaaata
    27541 atggcctttt cccttgcaag cagttgaaat caacagattg tcaaaataca tcctaaaaat
    27601 ttaataactt atatgattgc aaaggttgcg gcttgtatgc cggtcattat ggcggaaata
    27661 ccgtttggga ggcagccgca tcgcgtggaa tcggcacggc aggcgatgcg ccgatgcctt
    27721 cgggcgtgaa ggcggggcaa tcggcagttt ggaaaaaggc aatggaatct gtgggcagaa
    27781 gaagttaagg gtaaaagtcg ggcgcacggc caggttgtct gtccttttgt cggaacagga
    27841 gactgggcag ctttgtcctg attaattgct tgtgcaaacg gtaaaaaggg tttttcgtgt
    27901 gttttaaagc aggttgtaca gcatttttcc ggcaatcaaa agcagcataa tgccgaagga
    27961 ttttttgagt ttggcagaag aaagtttgtg ggcggttttg acaccgagcg gggcaaaggc
    28021 aatggttgcc gcgctgagga cggcgacggc gggcaggtaa aggaagccca gcgacccttc
    28081 gggtaatcct gcaatattca gaccgttgat cagatacgat attgcgccag agagtgcaat
    28141 cggccaggca aggccggatg atgtgccgat ggctttatgg gcggggaagc cgcagtggat
    28201 taagaaaggg acggaaagtg aaccgccgcc tatgccgacc cagctcgaca ttgcgccgaa
    28261 cagtgtggaa accgcagtca gtccgggcag tccgggcagc gggcgggatg ccgtctgagg
    28321 gccggtatgc agtgttttga atgcgacggc agttaaaaac aggatgaaga aaatttgaag
    28381 cccgaacgcg gggatatatt ttgcggagag tgcgcccgtg aatacgccga atatcatacc
    28441 cggcatcatc gcaaatacgg ttttccagtc gaccgcccgt tttttgtgct gccccaacat
    28501 actggaaaag gcggtgaaga ccatgacggc gaaggatgtg ccgacggcga ggtgttgcgc
    28561 gtaaggatgt tgtgccaaac cctgcaaatc aagcacccat aaaacgacag ggacaatcag
    28621 cgtgccgccg cctacgccga acaggccggc aataaaacct gccgcactgc ctacggcaag
    28681 caggattaag ataatgtccc aatgccacat ttaatccgcc tttctgtatt ttttccaaaa
    28741 tcggcgtgcc gccataatgt agccggacag gctgtatccg agaaagaaca ggaagaggac
    28801 gagcgacggt tcccaagtga ccagaagcag cagtaagacg gcaagcagca ttccgacaaa
    28861 ggggacttgt ctgcggatgt tgatttcttt aaaactccaa aaagggattt ggacaatcat
    28921 cgacaggccg gcaaacagtg tgatgcccaa tgcccaccag tggacggcgg ggaatttttc
    28981 gacgctgtgg ttgacccaaa tcagcccgac aatcagcgcg gcggcagtcg gactgggtac
    29041 gccgataaac cagcgtttgt ccactttgcc gatgagcgtg ttgaacaggg cgaggcgcag
    29101 ggcggcgcag gcgcagtaga tgaaggcgac ggaataaccg attttgccga actgccaaag
    29161 ctgccatttg taggcaatca gagcgggagc aacgccgaag ctgaccatat cggcaaggct
    29221 gtcgagctgc tccccgaacg cgctttggct gttggtcagc cgcgccacgc gcccgtccat
    29281 accgtccagc aacatagaga tgaataccgc gatggcggcg gtttcataac gtccgtgcat
    29341 ggattgggtg attgcgtaaa acgcggaaaa cagcgcggcg atggtaaagg agttgggcag
    29401 caggtagata ctgttttgac gcagggagcg ttgcggggtg ttggtcgggg tttccatagc
    29461 tgaagtgtct cacggacgtg gagaatcgat aggggattat aacccaaacc attatcgaac
    29521 aatacgccct ttaggatgaa gccctcggag gcggtttgtt tttctgatgg cggatgcttt
    29581 gcggggcggt ttgactctgc ctgccaccgg gtgtgttgtt tcagattttt gccggatgga
    29641 tattgttgcc gggcatttct gggcttgccg gaaggtatcg gggggagggc atcggaatat
    29701 gcggcgcgcc gttcaaaccg gggcggcgtt tccctatttt cagggggttg caggtttcaa
    29761 caaattgaaa gatatttttt gcatttccga ccgtatgctg attttgaaac gggataaatt
    29821 ataggacaag gtttttcgcc cgccgcaaag gttggccgca atgccgtctg aaaacaacgg
    29881 cttgccgcta aggcggcggt atgcggattt gcgctgccga aatcggtttg ccatacccgg
    29941 cggtgcaatc tgcggggagg gataagccga taaggcggga cttatcctgt tttttgttta
    30001 taatgcaaca gaatttggac gaaccgtcgg gaatttatca tgatgaaaaa gatactggcc
    30061 gtatcggcac tatgcctgat gactgcggcg gcacgggctg ccgatactta cggctatctc
    30121 gccgtttggc agaatccgca gaatgcggac gatgttttgc aggttaaaac cacaaaagaa
    30181 gattcgacga aaagcgaagc gtttgccgag ttggaagcct tctgcaaagg tcaggacacg
    30241 cttgcgggca ttgccgaaga cgagccgacc ggatgccggt cggtcgtgtc gctgaacaat
    30301 acctgtgtcg cgctggcata cccgaaagcc ttgggcgcga tgcgcgttga aaacgccgtc
    30361 gtgattactt ctccgcgttt tacgagcgtt catcaggtcg cactcaacca gtgcatcaaa
    30421 aaatacggcg tacagggaca atgcggcttg gaaacagtgt attgcacatc ttcttcttat
    30481 tacggcggaa ctgtgcgctc tttgattcaa aatctcaaat aaaacgggaa atgccgtctg
    30541 aaagatgttc agacggcatt tctatatcga cggtcaggat tctttcggat cgggcagcag
    30601 gctgttcaac ataatggcaa gtacggcgca caagcccacg ccggcaaagc tgaagctgcc
    30661 caatttgagc gtcatgccgc cgatgcccgt ggtcagtacc gagctgacga tgaccaggtt
    30721 tttcggcagc atcaaatcga ctttggcatc aatcagcgtt ttcacgccca aagaagcaat
    30781 cgtgccgaac agcagcagca taatgccgcc cattactggc atcggaatgg aagccaaaaa
    30841 cgcattgaat ttgccgaaaa acgccatgca gacggcaaaa accgccgccc aagtcatgat
    30901 gacggggttg ctgtttttgg taatcatcac cgcacccgtt acttcgccgt aggtcgtaac
    30961 cggcgggccg ccgatcagac ccgcaacgca tacgcccaaa ccgtcgcccg taagggtttt
    31021 gtccaagccc gggtctttcg tatagtcttt ccccgtcaca ttgccgattg ccatgatgcc
    31081 gccgatgtgt tcgatggcgg gggcgacggc aacgggcagc ataaacagtg cagcctgcca
    31141 gttgacctga ggcgtttcaa aatggggaac ggcgaaccag ggcgcgtgtg caatgcttgc
    31201 cgtgtccacc agtcccatca gcagtgccaa aacataaccc gaagcgacac cgatcaagat
    31261 gggaatcagc ttcatcatcc tgctgccgaa aaccgatacg atggcggtaa cggcaaaggt
    31321 aaagccggaa agaatcagcg aatcggtata gtcgatgacc tgtttgccgt ccgcctgacc
    31381 cattgccatg ctgcttgccg ccacggcaac agacagaccg atgaccatga tgacggggcc
    31441 gatgacgacc ggaggcagca gtttgtgtac cgctgccagt ccgcgccaac ggatcagcgc
    31501 ggcaaacaca aaatacataa agccggcggc aaacagtccg aacatggtgg aaggcagccc
    31561 ccattcgccg acggagtaga taatcggtgc gataaaggca aacgaagaac caagaaaaat
    31621 cggcactttg cgtttggttg cgatttggaa cagcagcgtt cccaagcctg cgcccaaaag
    31681 cgcaagagcc ggattcagac cggtcagcag gggaaccagc accattgcgc cgaatgccac
    31741 aaataaaatc tgcgcgccgg aaacggcaag tttcagttgg ttcataccat cttctttcaa
    31801 tcggttcaaa cggcacggat tatactggtt tgccgagagt ctgcgtaaaa atacatatgg
    31861 cgggcaacag atatgccgtc tgaaaggcaa tgtctgtctg ttgcaggatg aaagcaggag
    31921 aaatggagta taatccgaca ctttccatat tcaatccgca agccgtccgg cttccttatt
    31981 tcagacggca ttttccggta atacggcatg acagaaaaat cccataaaaa aacagcaaaa
    32041 ggcagggcgg gaagcccgtc cccaacgtcc gcacgcaaca agaaagccga caacggcgca
    32101 cggggcaata aagtttccga gcggctcaag gcggtcaaag agttgcagaa aaccgaaacc
    32161 aaaaaggcgc gtcccgaaca tgtcgtcaac cttatcggcg acgcactgtg gctgatgggt
    32221 ttggcggcaa ccctgtattt ggcgatttcc ctgatcagtt tcgatatggg cgatccgtct
    32281 tggtcgcaca gttcgccggt tgtggaagat gtcgccaatt ggggcggact gttcggcgcg
    32341 tatgttgccg atgtcggcta ttatcttttc ggctggtcgt tctggtggtg gatagcggct
    32401 gcctgcgtca tgctgtataa aaattttcgt ctgcacgcaa aacagacgga aaacgaggca
    32461 tacaaccaca aaatcgctgc cgccgcgctg tttgtcctga cggtcttcag ccccgtcttg
    32521 gagtattttg tgctgggcgg aaaatatgcc gactccctgc ctgtcggagc aggcggtatg
    32581 gtcggcatac gcgtcggcgc agtgtttgcg tggctgctgg ggaaatcggg cagcctgctg
    32641 attatcttgg ttgttctgct gttgtcgttg tccctgctgg tgcagatttc atggctggaa
    32701 tttttgaacg gtgcgggcag ggcggttcaa aaccgcctga gtgccttatc cggcaaggtc
    32761 atggctttag gaaaacgccg gccgaatagc aaaacagacg gtgtcgatac ccaaaataca
    32821 cggcgcatgg taaaagaagc caagaatatt acggccaaac ccgttgcctt gcccgaaggc
    32881 agcagcagca accgcaaatc cgtcgcggtt tccgtcgcgc cgtcgcccaa aattcaggtt
    32941 tctctgtttg aagatgacga acctcggcag gcgggcgaat accacaagcc tacattgaac
    33001 ctattgcgga ttcctgacag cgaacccgtc agcatcaatc ccgccgaatt ggagcgcact
    33061 gccgaactga tcgaatccaa actggcagaa ttcggcatcg gcgtacaagt cgtatccgcc
    33121 acatccggtc ccgtcatcac gcgctacgaa atcgaacccg cgcaaggtgt taaaggcagc
    33181 caaattgttg ccttgtcgaa agatttggca cgctctatgt cgctgcaatc cgtgcgtatc
    33241 gtcgaaacaa tcgcaggtaa aaacacgatg ggcatcgagt tgcccaacga caaacgccaa
    33301 gacgtgatgt tgagcgaaat cttgtcctcg cccgtgtttg ccgaagccaa atccaagctg
    33361 accgtcgcgc tgggcaaaga cattgccggt acccccgttg tcggcgactt ggcgaaaatg
    33421 ccgcaccttt tggtcgccgg tatgactggt tcgggcaagt ccgtcggcgt gaacggcatg
    33481 attatgtcta tgcttttcaa agctacgccc gacgaagtcc gcttcattat gatagacccg
    33541 aaaatgctcg agttgagcat ttacgacggc attccgcacc tgctctgtcc cgtcgtgacc
    33601 gatatgcgcg aagcagggca ggcgttgaac tggtgcgtcg ccgaaatgga aaaacgctac
    33661 cgcctgcttt cccacgccgg tgtacgcaat ttggaaggct tcaaccaaaa agtcgaagcc
    33721 gcaaaagcgg caggcaagcc gctgctcaat ccgttcagtc tgaaccccga caaccccgag
    33781 cctttggaaa aactgccgat gattgtggtc gttatcgacg aacttgccga cctgatgatg
    33841 accgaacgca aagccgtcga gcagcaaatc gcccgtctcg cccaaaaagc gcgcgccgcc
    33901 ggtatccata tgattgtcgc cacccaacgt cccagtgtcg atgtcgttac cggcctgatt
    33961 aaagccaaca tcccgacgcg tatggcgttt accgtgcaaa gcaaaatcga cagccgtacc
    34021 atcctcgacc aaatgggcgc ggacgaactg ctcaaatatg gcgattcgct gttcctccag
    34081 cccggcagtg ccgaaccgac tcgcctgcaa ggcgcgtttg tttcagacga cgaagtacat
    34141 caagtcgtca actacgtcaa atcgcaagcc ccagccgact atattgaagg tctgctcagc
    34201 ggcgaggccg cgctggaaac tgccaatatc gttaatccga atgcagacag cgacgaattg
    34261 ttcgatcagg cagtcgccta tgttttggaa agcaaaaaaa cctccatttc gtctttgcag
    34321 cggcagttgc gtatcggcta taaccgcgcg gcaaacctga tggaggcact ggaaaatgcg
    34381 ggtgtcgttt ctcccaccga cctcaacggc agccgtaaaa ttttggcgca caaggaccat
    34441 ttgtagctcg tattgcaaaa tgccgtctga acggcggaat tggcgtttca gacggcatat
    34501 tatgtttcag gcgaaacatt gtgatatact tgccagctaa aatttcccct ttgcggcaat
    34561 gcggttcaaa tatcgtaccg ttgcgctgtt tgcttcccca tgtagggaag aaagtttatc
    34621 attttatcaa cacaacaaat ttaagggctt atgatgagcg taactgttga aactttagaa
    34681 aatctggaac gcaaagtagt gttgtccctg ccttggtccg aaatcaacgc agaaaccgat
    34741 aaaaaactga aacaaaccca acgccgtgca aaaatcgacg gtttccgtcc gggtaaagca
    34801 cctttaaaaa tgattgccca aatgtacggt gcgagcgcac aaaacgacgt gatcaacgag
    34861 ctggtgcaac gccgcttcta cgatgttgcc gttgcccaag agttgaaagt ggcaggcttc
    34921 ccccgttttg aaggcgttga agaacaagac gataaagagt ctttcaaagt tgccgccatt
    34981 tttgaagtgt tccccgaagt cgttatcggc gatttgtctg cacaagaagt tgaaaaagta
    35041 accgcttccg tcggtgatgc cgaagtggac caaaccgtag aaatcctgcg caaacaacgc
    35101 acccgcttca accatgtcga acgcgaagcc cgaaacggcg accgcgtcat cattgacttt
    35161 gaaggcaaaa tcgacggcga accttttgcc ggcggcgcat ccaaaaacta cgccttcgta
    35221 ttgggcgcaa gtcaaatgct gcctgaattt gaagccggcg tagtcggcat gaaggctggc
    35281 gaaagtaaag acgttaccgt caacttccct gaagaatacc acggcaaaga cgttgccggt
    35341 aaaactgccg tgttcaccat tacgctgaac aacgtttccg aagcgactct gcctgaagtc
    35401 gatgcagatt ttgcaaaagc cttgggtatt gcggatggcg acgttgccaa aatgcgcgaa
    35461 gaagtgcaga aaaacgtaag ccgcgaagtg gaacgccgcg taaacgaaca aaccaaagaa
    35521 tccgtaatga acgcgctgct caaagccgta gagctgaaag cacctgttgc tttggtcaat
    35581 gaagaagccg cacgcttggc aaacgaaatg aaacaaaatt ttgttaacca aggtatggct
    35641 gatgctgcca acttggatct gcctttggat atattcaaag aacaagccga acgccgcgta
    35701 tctttgggtc tgattttggc caaactggtt gatgaaaaca aactggaacc gactgaggag
    35761 caagtcaaag ccgttgtcgc caacttcgca gaaagctacg aagatcctca agaagtaatc
    35821 gactggtact acgcagagcc ttcccgcctg caagccccga cttctttggc gatagaaagc
    35881 aacgtcgttg atttcgtttt gggtaaagcc aaagtaaacg aaaaagcttt gtcttttgac
    35941 gaagtgatgg gcgcgcaagc ctgattatcc tgaaaatgcc gtcttagggc agtttttgaa
    36001 agcaccgaag ccgcttttaa agcccgcttt ggtgctttta catcatgaaa ggagacgaaa
    36061 tgtcttttga taactatctt gtccctaccg ttatcgagca gagcggtcgc ggtgagcgtg
    36121 cattcgatat ctattctcgg cttttgaaag agcgcatcgt attcttggtc ggcccggtaa
    36181 ccgacgagtc cgccaatctg gtggttgccc aactgttgtt tttggaaagt gagaatccgg
    36241 ataaggatat tttcttctat attaactcgc caggcggttc ggtaacggcc ggtatgtcga
    36301 tttacgacac catgaatttt atcaagcccg atgtatcgac tttgtgcttg gggcaggcag
    36361 caagtatggg cgcgttctta ttgtcggcag gcgaaaaagg caaacgtttc gccctgccca
    36421 acagccggat tatgattcac cagcctttaa tcagcggcgg cttgggcggt caggcatccg
    36481 atattgaaat tcacgcacgc gaactgttga aaatcaaaga aaaactcaac cgcctgatgg
    36541 cgaaacattg cggccgcgat ttggcagatt tggagcgcga caccgaccgt gataatttca
    36601 tgtccgccga agaggctaaa gaatatggtc tgattgatca aatcttggag aaccgtgctt
    36661 ctttacagtt ttaataagaa atctgagaga aacaaatgcc gtctgaaaac agttcaggac
    36721 ggcatttgtc ttataggg
//
LOCUS       DAWFJW010000008        33998 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFJW010000008 DAWFJW010000000
VERSION     DAWFJW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.008"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(127..1047)
                     /gene="truB"
                     /locus_tag="WGR19_000313"
     CDS             complement(127..1047)
                     /gene="truB"
                     /locus_tag="WGR19_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0797912.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYTE
                     NGRFAGLAEYQKEICRLKALRLMNTAASAV"
     gene            complement(1105..1629)
                     /locus_tag="WGR19_000314"
     CDS             complement(1105..1629)
                     /locus_tag="WGR19_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797913.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGRHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRMLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            complement(1640..2011)
                     /gene="rbfA"
                     /locus_tag="WGR19_000315"
     CDS             complement(1640..2011)
                     /gene="rbfA"
                     /locus_tag="WGR19_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0797914.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELSKRIKLFKTPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            2201..3445
                     /gene="clpX"
                     /locus_tag="WGR19_000316"
     CDS             2201..3445
                     /gene="clpX"
                     /locus_tag="WGR19_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0797915.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESVGQTSEEPGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADVTKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFEP"
     gene            complement(3548..4744)
                     /locus_tag="WGR19_000317"
     CDS             complement(3548..4744)
                     /locus_tag="WGR19_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0797916.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVCSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHALTEFA
                     ATSDNP"
     gene            5148..5648
                     /locus_tag="WGR19_000318"
     CDS             5148..5648
                     /locus_tag="WGR19_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797917.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHPLPL"
     gene            5733..6287
                     /locus_tag="WGR19_000319"
     CDS             5733..6287
                     /locus_tag="WGR19_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797918.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            6301..6471
                     /locus_tag="WGR19_000320"
     CDS             6301..6471
                     /locus_tag="WGR19_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797919.1"
                     /translation="MPSEGLSDGIFAFVRGIVMLPFDFQTAFCFPAFDVGMAILIYVR
                     WQGGIVMPPLGF"
     gene            6611..6778
                     /locus_tag="WGR19_000321"
     CDS             6611..6778
                     /locus_tag="WGR19_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797920.1"
                     /translation="MNLRKFLIVAKAEKGFYYSLILWVSKPGGAETLQKCRLKLEIGF
                     RRHWFMRQVGA"
     gene            complement(6775..8163)
                     /locus_tag="WGR19_000322"
     CDS             complement(6775..8163)
                     /locus_tag="WGR19_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0797921.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFARLEVLILDEAD
                     RMLDMGFIDDIETIVEATPEDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKPAGGKPKG
                     KGGWGDRKAGGRRGDHKPGKEGFGGKTRGEGFKKESFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
     gene            8491..9438
                     /locus_tag="WGR19_000323"
     CDS             8491..9438
                     /locus_tag="WGR19_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0797922.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMRSEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES"
     gene            complement(9491..9823)
                     /gene="trxA"
                     /locus_tag="WGR19_000324"
     CDS             complement(9491..9823)
                     /gene="trxA"
                     /locus_tag="WGR19_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0797923.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            10121..10594
                     /locus_tag="WGR19_000325"
     CDS             10121..10594
                     /locus_tag="WGR19_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0797924.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLAS"
     gene            complement(10664..11485)
                     /gene="nadE"
                     /locus_tag="WGR19_000326"
     CDS             complement(10664..11485)
                     /gene="nadE"
                     /locus_tag="WGR19_000326"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0797925.1"
                     /translation="MMRIFSTGGTMDTQAVITHIARWLDEYATQANAKGFVIGVSGGI
                     DSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTADLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            11682..13037
                     /locus_tag="WGR19_000327"
     CDS             11682..13037
                     /locus_tag="WGR19_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0797926.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAMRLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPPRPQCIGIVTSLAAAALRDVVTTL
                     NRRAPEIPVIVYPTPVQGTGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACSIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGHLKTILEQRYFDASQKLDWLARQIRHPRQKLDDQRTYIHKLVQTLSYSMTQNLRA
                     HTARFERQTQTLKHCRPDVSVYKHNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13795..15336
                     /locus_tag="WGR19_000328"
     CDS             13795..15336
                     /locus_tag="WGR19_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0797927.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAIKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMASLLIIGLLCNLAVKSVHEKYH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(15405..16454)
                     /locus_tag="WGR19_000329"
     CDS             complement(15405..16454)
                     /locus_tag="WGR19_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0797928.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLIVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(16463..16618)
                     /locus_tag="WGR19_000330"
     CDS             complement(16463..16618)
                     /locus_tag="WGR19_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797929.1"
                     /translation="MHKLCLATSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAE
                     EPPYQAS"
     gene            16678..17310
                     /gene="pdxH"
                     /locus_tag="WGR19_000331"
     CDS             16678..17310
                     /gene="pdxH"
                     /locus_tag="WGR19_000331"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0797930.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(17440..18450)
                     /locus_tag="WGR19_000332"
     CDS             complement(17440..18450)
                     /locus_tag="WGR19_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0797931.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLSAPARQNLNLPHSCKNGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDNGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRVVHFYWGARHQDDLYALEEAQGFACRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(18542..19972)
                     /gene="gatB"
                     /locus_tag="WGR19_000333"
     CDS             complement(18542..19972)
                     /gene="gatB"
                     /locus_tag="WGR19_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0797932.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPELR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            complement(20015..20946)
                     /locus_tag="WGR19_000334"
                     /pseudo
     CDS             complement(20015..20946)
                     /locus_tag="WGR19_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
     gene            complement(20943..22388)
                     /gene="gatA"
                     /locus_tag="WGR19_000335"
     CDS             complement(20943..22388)
                     /gene="gatA"
                     /locus_tag="WGR19_000335"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0797933.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPIGV
                     QLIGNYFSEAKILGAAHQIQLNSDWHGKRPE"
     gene            complement(22451..22741)
                     /gene="gatC"
                     /locus_tag="WGR19_000336"
     CDS             complement(22451..22741)
                     /gene="gatC"
                     /locus_tag="WGR19_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0797934.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            22892..23545
                     /locus_tag="WGR19_000337"
     CDS             22892..23545
                     /locus_tag="WGR19_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0797935.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            23550..24893
                     /locus_tag="WGR19_000338"
     CDS             23550..24893
                     /locus_tag="WGR19_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0797936.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLVDLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTDRA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(24941..25375)
                     /locus_tag="WGR19_000339"
     CDS             complement(24941..25375)
                     /locus_tag="WGR19_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0797937.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKKIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(25489..25596)
                     /locus_tag="WGR19_000340"
                     /pseudo
     CDS             complement(25489..25596)
                     /locus_tag="WGR19_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(25815..27071)
                     /locus_tag="WGR19_000341"
     CDS             complement(25815..27071)
                     /locus_tag="WGR19_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0797938.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTKFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTHIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLMYATCSVLPEENELQVERFLSEHSEFELVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(27107..27919)
                     /locus_tag="WGR19_000342"
     CDS             complement(27107..27919)
                     /locus_tag="WGR19_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0797939.1"
                     /translation="MNYALDALWWKLTDPTVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLARHAPFGHRLGIYAEELSAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGGTGRPEDMRGLDPTDTLLGKAAKLTA
                     QLGLPLDVKPVSIVRGIGFFPQSFHAFEPPLNPYGWRGIYIQDWVEYGFERQEARYHL
                     LDRMAYLAPARVAETEILNATEIRRIDQGLIAVLELRPDGFWHEIERIMKAV"
     gene            complement(27990..28808)
                     /locus_tag="WGR19_000343"
     CDS             complement(27990..28808)
                     /locus_tag="WGR19_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0797940.1"
                     /translation="MTALKPALPAYLNNIRIVLTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFNPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTLQQRLKDGQS"
     gene            28872..29042
                     /locus_tag="WGR19_000344"
     CDS             28872..29042
                     /locus_tag="WGR19_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797941.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            29052..29837
                     /locus_tag="WGR19_000345"
     CDS             29052..29837
                     /locus_tag="WGR19_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0797942.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATSRLDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            30107..32230
                     /locus_tag="WGR19_000346"
     CDS             30107..32230
                     /locus_tag="WGR19_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0797943.1"
                     /translation="MGKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFG
                     GESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNP
                     EGAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLR
                     HGDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLF
                     GAPDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVC
                     LNATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLTARGEFETSPVTH
                     NWSTAFDRVIRQRKTIRGTAAGAGKVEVKANENIANQLASFKADYPNSWENSANLDAN
                     IKVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQP
                     DLVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKR
                     PGYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANL
                     LNNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLN
                     SNVSYFGKSYQDTQKQYAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQ
                     RGQYDRSFAVVGMPRTYWLKAELDF"
     gene            32354..33904
                     /locus_tag="WGR19_000347"
     CDS             32354..33904
                     /locus_tag="WGR19_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0797944.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         7583 a         8580 c         9574 g         8261 t
ORIGIN      
        1 aaatcaatca tttgttatct ttgctgtttc cgggggcgtt cgcaaagttg caataagcgg
       61 ggtgggtgtc cgagtgtgtc gtgattttgc ggaaatatca acatattatt caaacacagc
      121 tgccgttcag acggcggatg ccgccgtgtt catcaggcgc aaggctttca gacggcatat
      181 ttctttttga tattccgcca gaccggcaaa gcggccgttt tccgtgtaaa cgcgtaccgg
      241 cgtgtcggaa ggcaggtctt cttcgaaacg cggacgttgt ccgcagtgga gcatatggac
      301 ggcataatcg tttaaaacgg tttggggaaa gtgtgaaacc aatacgtcgc agggtagcag
      361 caagctgtcg cgttctgttt cgttcaaatt tgccaaggcc tcaagcgtgt ggctttgggc
      421 gatggtaaag ccggcggttt cagtgcggcg cagggcggtc aggtgggcga acgtgccgat
      481 gtgtttggcg atgtcttcgc tgagggtgcg gatgtaggtg cctttgctgc aacgtacgtc
      541 gatgacggct ttgggcgcgt caaattcggc aatatcgatg gcgtaaacgg taatgtagcg
      601 cgctttgcgt tcgatgacga tgcctttgcg ggcgtattcg tacagcggtt tgccttcgtg
      661 cttgagcgcg gaaaacattg gcggcacttg gcggatgtcg cccgttaatg ctttgcaggc
      721 cgtctgaaat tcggctaagg aaatatcggc acgggcggtg gcaacgattt cgccttcggc
      781 atcacccgtg ctgctggctt cgccgagttt cagcgtggcg gtgtaggctt tgtcggcatc
      841 cagcaggtat tgggcgaact tggtcgcttc accgaagcag acgggcaaaa gtccggttgc
      901 caaagggtcg agcacgccgg tatgtccggc tttttcggca tgaaacaaac gccgcgcttt
      961 ttgcagcgcg gtgttgctgg aaaggccttc gggtttgtcg agaagaagaa cgccgttgac
     1021 cgggcgtttg gcgggtttat tggtcatatt aattagggaa tattaatgtt cagaccgtct
     1081 gaaagggttc agacggcctg cgtgttagct taattcttca ttcaataatt cacgaacaga
     1141 gtgtttttca tcaattagat caacgctgat tgcatcaaag ccatttcgtt ttaatgagtc
     1201 aaaactaatg attcggccat caattaaggg gacttgtcct tcagaattga cgaaatcttc
     1261 atattcatct tcatagtcta tattgtaata aagatgaatc agaactcggt aagtatactc
     1321 atccaaatca gttaacattc tgttttgctt ttgatttgga ctatatttgt cgcaaaacat
     1381 agcgttatag ccatgttccg cgccatcaaa caatacgatt tcatcgccgc ttacagcttc
     1441 taccgccaca atttttaccg gagccttatc agtatcggta atcaatccat caaaaggctc
     1501 tccatagtaa cggatgtgga aagtatctct accttggcag gataatgaaa agcaacgatg
     1561 cctgccttca atttgcaggc tatcactatg tgcataggcg gctaaatgtt cgggaatcag
     1621 attgttcatt ccagcagtat cagtcttcaa ccggtttttc cgccgccact tgatcgataa
     1681 gggcagacag gttcatacca cgctctaagg attcgtcgta tttgaaatgc agttcgggcg
     1741 ttttgaacag tttgatgcgt ttggacaatt cgctgcggag gtgtccgcgc gcgtgttcca
     1801 gcacttcttc cgtaatttcg cgcgcgtctt ggtttaacac cgtatagaac acggtggcat
     1861 ggctgtaatc tcgggtaact tcgacttcgt tgacggtaat gaagccggcg cgcgggtctt
     1921 tcagtccggt acggacgagt tcggcaagct cgcgcataat ttgttctttg acacggtctt
     1981 ggcgggcata gccgcgttgg ggttttctca tgaatatcct cagatttcag atttggtttc
     2041 agacggcatt tttgccgccg gggcggttgc gaaagggcgt attatagcct aatcggcggg
     2101 attttaaggt aatacattga taagaacgga tatagttatt atacttggca acccgaacgg
     2161 cacgtgccgt ttctgtaacg catacattgt aaagaaagcc atgtccaacg aaaaccgtac
     2221 ctgttccttt tgcggaaaat ccaaatcaca cgtcaaacat ttaattgagg gcgaaaacgc
     2281 ctttatctgt gacgaatgcg tctcaaactg tattgaaata ttgcatgaag acggaaatga
     2341 cggcacgcct tcggaaagtg tcggacagac atcggaagag cccggcaagc tgcccacgcc
     2401 cgccgaaatc gtcgccaacc tcaacgacca cgttatcggt caggaacaag ccaagaaagc
     2461 cttggcggtg tcggtgtaca accattacaa acgcctgcgc cacccgaaag ccggagctaa
     2521 tgtcgaattg tcgaaatcca acatcctgct tatcggcccg accggatcgg gtaaaaccct
     2581 gctggcgcaa tctttggcgc gcaaactgga tgtgccgttc gtgatggcag atgcaaccac
     2641 gctgaccgaa gcaggttatg tcggcgaaga tgtcgagcaa atcatcacca aactcttggg
     2701 caaatgtgat ttcgatgtcg aaaaagccca acgcggcatc gtctatatcg acgaaatcga
     2761 taagatttca cgcaaaagcg acaatccgtc catcacgcgc gacgtgtccg gcgaaggcgt
     2821 gcagcaagcc ttgctgaaac tgattgaagg tacggtggcc agcgttccgc cccaaggcgg
     2881 ccgcaagcat ccgaatcagg aatttatcaa cgtcgatacc accaacatcc tgtttatctg
     2941 cggcggcgcg tttgcaggct tggaaaaagt catccgccag cgtactgaaa aaggcggtat
     3001 cggtttcggt gcgtccgttc acagcaagga cgaaaatgcc gatgttacca agctgttcgg
     3061 catcgtcgaa ccggaagatt taatcaaatt cggtctgatt ccggaattaa tcggacgttt
     3121 gcccgtgatt gcaacgttag aagaactgga tgaggacgcg ctgattaata ttttgaccga
     3181 gccgaaaaat gctttggtca agcagtatca agctttgttc ggtatggaaa atgtcgagtt
     3241 ggaatttgaa gaaggcgcat tgcgttccat cgcgcgtcag gcaatggagc gcaaaaccgg
     3301 cgcgcgcggc ctgcgttcca tcgtcgaacg ctgcctgttg gatacgatgt accgcctgcc
     3361 cgatttgaaa ggcttgaaaa aagtggtggt cggtaaggca gtcatcgaag agggcaggga
     3421 accggaattg gtgttcgagc cctgatggca aacaaaaccg cttcggaacg tcgaaccacg
     3481 tttccgaagc ggttttttgt ttttgatgcc gtctgaagcc ttcccgcctt cagacggcat
     3541 ttgagtttta cggattgtct gatgtcgcgg caaattccgt cagcgcgtgt tccagacgcc
     3601 gcaaaccttc cgccgtatct tcatcgtcca acagcagcga aggcgcgaaa cgcaatacgt
     3661 ccgcacccgc aaccaaaatc atcacgccgt gtttcaaggc ggcggcggtt atttcggatg
     3721 cgcgtccgcg ataaggcgcg tccaacacgc agccgagcag cagccccatg ccgcgaactt
     3781 gcttaaacac gcccgtttgt tctcccaaag accgcaatgc cgtctgaagt ttctgcccct
     3841 gactgcagac gttgtgcaag gtttccggcg cgttgatgat gtcgaaggcg cggctgccga
     3901 ccgcacacgc catcgggttg ccgccgaaag tcgagccatg cgtgcccggt tggaaggcgg
     3961 cggcaatcgt ttctgtcgcc agcatcgcgc cgatcggaaa gccgcagccc aaggcttttg
     4021 ccgaactcaa aatatcggga acaacgccgt aatgttcata ggcaaacagc ctgcccgtat
     4081 gccccatccc ggtttgaact tcgtccaaaa tcaacaacgc attgtgccgg tcgcacagac
     4141 ggcgcgcggt ttgcaaatat tccgcagtgg cgggcaggat gccgctttcg ccctgtatcg
     4201 gctcgatgat gaccgcgcag gtctgttcgc cgacggcagc ttccagcgcg gcaatatcgt
     4261 tgaacggaac gtgcgtaatg ccttgcggca ggggtgcata atccttgctg tatttcggct
     4321 gaccgccgac ggacacggta aacagcgtgc gtccgtgaaa actgttgata caggcgacga
     4381 tttcgctttt tcctccgccg aaacggtcgc gggcgtattt cctcgccagc ttcaacgccg
     4441 cctcattcgc ttccgagccc gagttgcaga aaaaaacctt gtcggcaaaa ctgtttgcaa
     4501 ccaatttttg cgccaattcc tgcgctggac gcgtcgtata gatattggaa atgtgccaca
     4561 gcttgtgcat ctgcgcgttt aaagcatcga caagggcagg gtggcagtgt cccagcgcat
     4621 tgacggcgat accgcctgaa aaatcaatat attcacgccc tttcgtatcc caaacgcggc
     4681 tgcctgaagc gcgttcggga atcatcgggg caaaggcgaa attgggggtc agatagtttt
     4741 gcataattgc tcctgattgc ggttttgctc gggattatgc accgtttttc aaagcagata
     4801 aagggaaatt gccgacaata tcgggcagcg gtaaaaataa accgaaatat tttcaatcgg
     4861 atagatgaaa aatcaaaaaa atttcgataa aatgcttgag gcgcggcagc gttttgattt
     4921 agaatctccc caatattttt atcttcagac ggcatttcat catgcaaggc aaaaaattta
     4981 caaccgctta ttgctattgg taccacacag acgtgcggta cttttttgcg cttgcttgaa
     5041 atccatccca tacgcaaaac aggccgctcg aaaacggggc ggcttttttg ttaccttcac
     5101 acaggtcgcc cgattcggca acacacgaca gaatcaggaa aatgaccatg aatgcaatgc
     5161 cccaaaccgc cgcacaaaac gaaaccctgc acacttgtag cattattgtc gatgatgcac
     5221 aggatttttc ccccatgctt ctcgatgcag ggcgggacga cacacttatc agcgaatcga
     5281 tgattccgca aatccgcacc gttgccgccc tgatagccgc cgaacggcac gacttcagcc
     5341 gcagcagccc cgccgagttt accgatgccg ccgatttttt tgccgcacgg attctcgtat
     5401 tgggcgtgcg acgcttccat ctggacgtat cgctgcttca gattttgaaa accgccaaca
     5461 gccgcgcccg ccgctttgcc gaaaaacacc gcctgttttt cacacccgca caagccgaat
     5521 tgagcctgaa caaacacaaa aaccgccgcc tgctgaccgt agaaaccgaa cacgaagtcg
     5581 aaaacaaagg caacccagtc gccaacagcc tcgcatttgt ccgcaaattg caccccctgc
     5641 ccctgtaaac atcacgattt gccggcagaa tgcttgccaa aattcccgac ggcgatacaa
     5701 taaaaccgat tttccaccca aacaggacac acatgaaaaa aatcatcgcc tccgcgctta
     5761 tcgcaacatt cgcactcgcc gcctgccaag acgacacgca ggcgcggctc gaacagcagc
     5821 agaaacagat tgaagccctg caacagcagc tcgcacagca ggcagacgat acggtttacc
     5881 aactgactcc cgaagcagtc aaagacacca ttcctgccga agcacaggca aacggcaaca
     5941 acgggcaacc cgttaccggt aaagacgggc agcagtatat ttacgaccaa tcgacaggaa
     6001 gctggctgct gcaaagcctg gtcggcgcgg cggcaggcgc gtttatcggc aacgcgctgg
     6061 caaacaaatt cacacgggca ggcaaccaag acagtcccgt cgcccggcgc gcgcgtgcag
     6121 cctaccatca gtccgcacgc cccaatgcgc gcaccagcag ggatttgaac acgcgcagcc
     6181 tccgtgcaaa acaacaggcg gcgcaggcgc agcgttaccg cccgacaacg cgcccgcccg
     6241 ccaattaccg ccgccccgct atgcgcggtt tcggcaggag gcggtaaacc cggcgtgcca
     6301 atgccgtctg aagggctttc agacggcatt tttgcatttg ttaggggcat tgttatgttg
     6361 ccgtttgatt ttcagacggc attttgtttc ccagcgtttg atgtcgggat ggcaattctg
     6421 atttacgtgc gttggcaggg cggcattgtc atgccgccgt tgggttttta gacggcatca
     6481 tatcgaaccg tcagaccata gggcgagcat tcttgcccac cgttgcaagg gcgggatttg
     6541 gtgggcagga atgcccaccc tgcatctgaa cctttccgcg tcattctcgc gcaggaagga
     6601 atttaggtat ttgaatttac ggaaattttt aatcgttgcc aaagcggaaa agggttttta
     6661 ttacagccta atcctttggg tatcaaagcc gggcggtgct gaaacattgc aaaaatgccg
     6721 tctgaaactc gaaatcggtt tcagacggca ttggtttatg cggcaggttg gggcttagcg
     6781 gtctttgtaa cggcgttcgc ctctgcctgc aaaagaatcg ccggctttgc gtttgccttt
     6841 gaagccttcg ccggtttttt tgaaaccgtc tttcttaaag ctttctttct tgaaaccttc
     6901 gccgcgcgtt ttgccgccga agccttcttt gcccggttta tgatcgccgc gccggccgcc
     6961 ggctttacga tcgccccagc cgcctttgcc tttcggtttg ccgccggccg gtttgcgttt
     7021 gcgtgtcggt tccatgcctt cgatggtcag ttcgggcagt ttgcggttaa tgtatttttc
     7081 gattttgtgg actttgacgt attcgttcac ttcggcaaac gtaatcgcaa tacccgtgcg
     7141 gcctgcgcgg ccggtgcgcc cgatgcggtg gacgtagtct tccgcctgtt tcggcaggtc
     7201 gtagttgatg acgtgggtaa tggtcggtac gtcgataccg cgtgcggcga cgtcggtggc
     7261 aaccaaaatt ttgcagcggc ctttgcgcaa gtccatcagc gtgcggttac gccagccttg
     7321 cggcatatcg ccgtgcaggc agttggcggc gaaacctttt tcgtacaatt catccgcgat
     7381 gacttcggtc atggctttgg tggacgtgaa aatcacgcat tggtcgatgt tggcatcacg
     7441 caggatgtgg tcgagcaggc ggtttttgtg gcgcatatcg tcgcagtaca gcagttgttc
     7501 ttcgattttg ccttggtcgt ccacgcgttc gacttcgatg atttcagggt ctttggtcag
     7561 tttgcgtgcc agtttgccga ccgcgccgtc ccaagtggcg gagaacaaca aagtctgacg
     7621 gtcttccggc gtggcttcga cgatggtttc gatgtcgtcg ataaagccca tatccaacat
     7681 acggtcggct tcgtccaaaa tcagcacttc caagcgggcg aaatcgactt tgccgctttg
     7741 catcaagtcc atcagacggc ccggcgtggc gacaatcaga tcgaccggtt tgctcagggc
     7801 gcgggtttgg tagccgaacg atgcaccacc gacgatgctg acggtacgga accaacgcat
     7861 atttttggca tacgccagcg cgtttttctc gacttgagcc gccaattcgc gggtcggcgt
     7921 caacaccaac gcgcgcgggc ctttgcccgg tttttcgctg cgtttggtca gtcgctgcaa
     7981 agtcggtaac agaaaggcgg cggttttgcc ggagccggtt tgcgccgaag ccatgatgtc
     8041 gcggccttcc aaagcaaacg gaatggcttg cgcctgaatc ggcgtcgggc tttcgtaacc
     8101 ctcgctgctg acggcggaca aaatgttttt atcaaggttc aaatcggcaa atttaataga
     8161 catggctatc cttggaagac aacaacgctc gcgtccgaaa ggttcagacg gcctgaagca
     8221 gggaagggta acgatacggg gaatgtgaag ttacagggtt gtcgcaagcg gcttgcttgt
     8281 ggtttacatc gacggcaacc tgcacagata cgctttgcaa aatcaaagca acagaagaaa
     8341 cgcgcccgta agggcggttt aggttggaag gcgatggctt cgtatgacaa gataagcgac
     8401 ggatggtata gggtttgccc gaaacggtca agcaatcggg cgttaattac agtaaaattg
     8461 ccgttttcca ctttattccg cctgaaacca atgaccgaca tcacaccgtt tgccaaccgc
     8521 ttgggcaaaa acatcaaaca ccttatgaaa tgggcgaaac gcaacggcat cgaagcctgg
     8581 cgcatttacg accgcgacat cccccaattc ccctttgctg tcgatgttta cggcgaccaa
     8641 atccaccttc aggaatacga taccggctgg ctgatgcggt ccgaagaata cgaagcgtgg
     8701 cttgccgaag tcttggaagc cgtcgccttc gttaccggtt ttgcgcccga acaaatccgc
     8761 ctcaaacgcc gcgaacgcca aaaaggtttg cagcaatacg aaaaaaccgg caaggcgggc
     8821 gacgatttcg tcatcaccga aaacggccgc aagttttggg tcaaccttga taagtatttg
     8881 gatacggggc tttttttgga tcatcgaaac acgcgcaaaa aagtcggcga aacggcagcg
     8941 ggcaaacgtt ttttaaacct gttttcctac accggcagct tcactgtcta tgccgccacc
     9001 ggcggcgcgg catccagcga aactgtcgat ttgtccaaca cttatctcga ttgggcgaga
     9061 cgcaattttg aattgaacgg catcgatacg gaacggcaca aaatcgtccg cgccgacgtg
     9121 ttccaatatc ttcagacggc atatggggaa ggcaggcagt tcgacctcat tgtgatggat
     9181 ccgcccagtt tttccaacag caagaaaatg tccgacatcc tcgacattca gcgcgatcac
     9241 aaaaagctga ttgacggcgc ggtgaagctg ctcgcttcag acggcatttt gtacttttcc
     9301 aacaacctgc gcagcttcgt cttagacgac ttggtatcgg aacaatacgc cgtcaaagac
     9361 atttccaaac aatccgttcc cgaagatttc cgcaacaaaa aaatccatag gtgctgggaa
     9421 atccgccacg aatcttaagg gtgcgaaaaa gccgccggaa tcttccggcg gctttttcga
     9481 ttgcgcttta tcaggcgata gaggcttcga caaaggcggt cagctgacct tttgccaatg
     9541 cgccgacttt ggtggcgacg acgtcgccgt ttttgaacac catcagggtc ggaatgccgc
     9601 gcacgccgaa acgggacggg gtggcttcgt tgtcgtcgat gttgattttg actactttca
     9661 gacggccttc aaattcggcg gcgatgtcgt ccaaaatcgg ggcaatcatt ttgcaggggc
     9721 cgcaccacgg agcccaaaag tccagcagga cggggaggtc ggatttcaaa acatcttgct
     9781 cgaaagcggc atcgccggtg tgtacgatta attcgctgct cattattttt ccttttcggt
     9841 tgggattggc acgatagcgt gcaggatagg gcttgggcat aaaaatttca agtgggcggg
     9901 cgggataata gtttttctgt accgccccaa tcgcctttgc aaattctaaa aaacggttcg
     9961 gacagtattt tatttctgaa aattactttt attatcaaaa tgtaaggaat acgtttgagg
    10021 ctgtacagaa gcgcgcgggc ggtgtatgct ccgtttgaat tgattcttaa agtatgaatg
    10081 ttcaaaacca aacccgaaac cctgtaaaag gaacactgcg atgactgatt tttccgtttg
    10141 ggagaccgcc ccctttggtg caaccgtcga tcatatcctg caacgctacc acaatgtcca
    10201 ccgcgcacaa tttgaagaat tggtgccgct ggcgcagaaa gtggcccaag tccatgccga
    10261 tacctttccg gcagaaattg ccgggctgct tgccgatatg cagaacgaac tgctgatgca
    10321 tatgatgaag gaggagcgga tgctgtttcc gatgattaat cagggtgtcg gacgcggtgc
    10381 ggcgatgccc atcggcgtga tgatgcacga acacgaagaa cacgaccgcg ccatcgcacg
    10441 gctcaaagag ctgaccgaca atttccaacc gccagaaggg gcttgcggaa gctggacgcg
    10501 gctttacgcg ctggcgaaag aaatggtgga ggatttgaac gaccacattc atttggaaaa
    10561 cgatattttg tttgcccgcg tacttgcttc ttaaggaacg ggagccggat atgtcaatgc
    10621 cgtctgaaac acggttttcc gcttcagacg gcattttcat gtttcagccc aacaattcgg
    10681 gcggaatgcg gcacacggga atcgggttga ttttgtgctg catcgcgcgg tgaagccgcg
    10741 tatagatttc caaaacttcg cgccgccgcc cttcaaaatc ttcgggtttg cgcgtaccgt
    10801 acacgcccat tgcccactcc agttcgggat agcttgcgcc catctgttct tcgtcggtgc
    10861 gttccgtatc ccacaggccg tcggtcggcg gggctttttg aatcgcctcg tccacgccca
    10921 atgcttcggc aagccggtaa acctgcgttt tggtcaggtc ggcaatcggg ctgatgtcta
    10981 cgccgccgtc gccgtatttg gtaaaaaatc ccacgccgaa atcttcaatc ttattacctg
    11041 tccccgtaac cagcagtccg tgtatctgcc cgtagtagta cagggtcagc atacgcaggc
    11101 ggcttttggc gttggcgagg gaaagcggct gattgtcaaa tgccgtctga tgaatgccga
    11161 cggtttgttc aaaggtctgg aaggtgtcgg tcaaatcggc cgtttgcgcg cttacattgg
    11221 catattgccg ttgcagattg cggatgtgca gccttgcccg ctcaagctgg ccggggtgtt
    11281 ggcgtatcgg catatccaga agcagcgtgg ggcggccggt gcgggcggcg agtgtggaga
    11341 cgacggcgga atcgatgccg ccggaaacgc ctatgacgaa tccttttgca tttgcctgag
    11401 tggcgtattc gtccaaccat cgggcgatat gtgtgataac tgcctgcgta tccatggttc
    11461 ctcctgtcga aaatatccgc atcatagctt gtcggcggtt caatttgaat agattgcagg
    11521 caggttgatg aatatttttc ccattccccg cttaaaaaag tcatacgggc aaagtcgggc
    11581 ggcaagatgg cgcgtgttat aatattgttg acaatttagg cggctctatg ccgccttggg
    11641 cttcaaaaca tattcatatt ttttactatt tgattgattc gatgtctgat tttttccatt
    11701 ctgacgtgtt gtccgtttcc gagttgaacg catttgccaa aagtattttg gaaaaccacc
    11761 ttgccggtct gtggattgcg ggcgaagtat ccaacctgac ccgtgccgcc agcggacatt
    11821 attacttctc gctcaaagac agccgtgcgc aggtgcgttg cgcgatgttt aagggtgcgg
    11881 ccatgcgctt ggcgaaacct ttgaaagaag gcgaccatat cgaggtatca gggaaaatca
    11941 gcatttacga ggcacggggc gaatttcaga ttaccgtgaa cgaggtgcgg ctcaaaggtt
    12001 tggggcagct ttacgaagcc tatgagcgat taaaagcaca gttgcaggcg gaaggcgcgt
    12061 ttgcggcgga acgcaaaaaa ccgttgcctc cccgcccgca atgcatcggc atcgtaacca
    12121 gtctggcggc agcggcattg cgcgatgtcg tgaccacctt aaaccgccgc gcgcccgaaa
    12181 ttcccgttat cgtttatcca acgcccgttc aaggcacagg tagtgagttg caaattgccc
    12241 aagcgattaa aaccgcttcg caacgcgccg agtgtgacgt gctgattgtc tgtcgcggcg
    12301 gcggcagcat cgaagacttg tgggcgttta atgaagagcc ggtcgtacgc gccatcgaag
    12361 cctgctcaat tccggtcgtc agtggcgtag gacacgaaac cgatttcacg cttgccgatt
    12421 tcgtcgccga cgtacgcgca ccgacaccga ccggcgccgc agaattggtc agccccaacc
    12481 gccaagaatc gttacaccgc ctcgcccaag cgcaaggcca tctgaaaacc attttggaac
    12541 aacgctattt cgatgccagc caaaaactcg actggctggc gcggcaaatc cgtcatccgc
    12601 gccaaaaact tgacgatcag cgcacctaca ttcacaaact ggtgcaaacc ctgtcctact
    12661 cgatgacgca aaacctccgc gcccataccg cccgttttga acgccaaacc caaaccctga
    12721 aacattgccg tcctgatgtt tccgtttaca aacacaacat cgaccgcttt cagacggccc
    12781 tgtcgcacgc cttccaccaa ctgcttaccc accgccgcca aagcctgacc gcccaagcag
    12841 cattgctcga agccgtttcg ccgcagcaca ttctggagcg cggcttttcc gtcgtcaaaa
    12901 acacacgcgg acaagtcatc cgcaacgccg atgttttgaa gcaggggcag aagctgcaca
    12961 ttatcttcgc cgacggcgaa accgacgtgc gcgtaagcaa agaacaaggg cagcaggatt
    13021 tgttcgattg catttaaacc caatcggaaa aaccgctccc cctccgccgt cattcccgcg
    13081 aaagcgggaa tccagtccct tgaccttaag ataatcttta attcgtcatt cctgaaaaag
    13141 cgggaatccg gaagcgaaaa gtggtgggaa tgtattagaa atgactgaaa ctcaaaaaac
    13201 cggattcccg cctacgcggg aatgacgagt cagaagttac tcgaaactta aaaaaaacga
    13261 aatcgaacgg actggattcc cgcctgcgcg ggaatgacga gtcagaagtt actcgaaact
    13321 taaaaaaccg aaatcgaacg aactggatgg attcccgcct acgcgggaat gacgagtcag
    13381 aagttactcg aaacttaaaa aaccgaaatc gaacgaactg gatggattcc cgcctgcgcg
    13441 ggaatgacga ttgatacgtt tcgataattt attgaagatg gctgaaatcc aaagttacag
    13501 aaatttataa acaaaaaaca gcaacccgcc gccgtcattc ccgcgaaagc gggaatccag
    13561 tcccttgacc ttaagataat ctttaattcg tcattcccgc aaaagcggga atccggaagc
    13621 gaaaagccgc aggaatttgt cggaaaaaac agaaaccttt ccgtacaccc gaaatgtttc
    13681 agacggggca tcttggggcg gataaaaaac cgatccgata tttttttata gttttcaata
    13741 agtttccatt gagcaaatga aattttattt ttcaactgac agaaagagga tgttatgaag
    13801 tttttagacc gtgaggcaag cattgccaag ccgggtttca accgttggct cgtaccgccc
    13861 gcagcattgg cggtgcacct tgccatcggg cagatttacg cctattccgt attcaacgcg
    13921 ccgctgacca aacttatcgg cataaccgaa tcggcggccg gagattggaa gctgacgacc
    13981 gtaggttgga ttttcagtat cgcactcgcg atgctgggcg cgtcggccgc cctgttcggt
    14041 acgtggatgg aacgggtagg gccgcgcaaa gccatatttg ccgccgcctg ctgcttcagc
    14101 ctgggcttct tcgtatccgc attcggcgtg cgtacgcaca acctcttcct gctctatttg
    14161 ggcaacggcg tcatcggcgg cgtaggcttg ggtttgggct acatcgggcc ggtttccaca
    14221 ctgatgaaat ggtttcccga caagccgggt atggcgaccg gtttggcgat tatgggtttc
    14281 ggcggcggcg caatgctggc ctcgccgctt tccgtatcac tgatgaacgc cttttcaaac
    14341 gctgcttcgg ttggggttgc cgaaaccttc gccgtattgg gtctgcttta cctcgcactg
    14401 atgatgtttg gcgcgtttac catccgcgta cctgccgacg gctggaaacc tgaaggctat
    14461 accgcgccga aaacgcaaaa caagctggtc agcagcaatc atgtcaacgt gtcccaagcc
    14521 attaaaacgc cgcagttttg gctgttgttc tgggtattgt gcctgaacgt aactgccggc
    14581 atcggcgtat tgggtcaggc atccgtgatg attcaggaac tcttttccga aacctccgta
    14641 ggtaggcagg cggcagtcgg cgcaggtgcg gcggcaggct tcgtcagcct cctgagcctg
    14701 tttaatatgg gtgggcgctt tttgtggtcg agcgtttccg acaaaatcgg acgcaaaaac
    14761 acctacacca tcttcttcgt actcggttcg ctgctgtatt tcgccgttcc ctccatcggc
    14821 gagggcggca gcaaagccct gttcatcatc ggcttttgcg tgattatttc catgtacggc
    14881 ggcggatttg ccgccattcc cgcctacctg aaagacctgt tcggcaccta tcaggtcggc
    14941 gcgattcacg gacgcatcct gctggcctgg tcaaccgccg ccgtcatcgg cccggtactg
    15001 gtcaattaca tacgccaaag ccaaatcgac agcggcatac cggcggcaca ggcatacagc
    15061 gtaaccatgt acatcatggc gagcctgctg attatcggtt tgttgtgcaa cctcgccgtt
    15121 aaatccgtac acgaaaaata ccacgaaaaa gacatcaaga cagccgcacg cagcggtaac
    15181 cccgacgacg aaaccgccat atccgacgct taccttgtgg gcgaaaaagt ttccggcggc
    15241 ggcatatcgg tctggtggcg ttgggcattg gccgtcatac cgctcgccta cggtgtggtg
    15301 atggtctttg tcaaagcact cgaccttttc tcctgaaaac gcccgaacaa aatgccgtct
    15361 gaaacagcag gtgtttcaga cggcattttt gtgttttggc gggtttaggc ttttttgcgg
    15421 cggcgttcgc ccggcagcag catttccgcc catttgagga tgttggcgac ttcggcgggg
    15481 ttgagttcgt agaactgtcc gcgtttgagg cggttgggca gtccgatggg gccgaagccg
    15541 acgcgcacga ggcggctgac ggtgagtcct tggctttcaa aaatgcggcg cacttcgcgg
    15601 ttgcggcctt ctttaatcac gacgttgtac catttgttcg cgccttcgcc gccttgttcg
    15661 cggatgcgtt cgacttttgc caagccgtct tcgagcatca cgccttcttc ggtgaggacg
    15721 cgcatttgtt cgccggtcag cccgcccaag acgcgcacgg cgtattcgcg ttcgacttcg
    15781 aagctggggt gggcgaaacg ttggacgagt tcgccggagg tggtaagaat cagaagtccg
    15841 ctggtgttga tgtccaagcg tccgatggcg acccagcggc tgctggcggc ctgcggcagg
    15901 cggtcgaaaa tactgaccct gccttgcggg tcgtcgcggg aaacgatttc gccttcttgt
    15961 ttgtaataca ggatgatgcg cggcaggcgg tcggcccatt tgagcttgat gatgctgcct
    16021 ttgacggtaa cgtggtcgtc gggggtaact ttgtcgccca gttgcgcggt tttgccgttg
    16081 accgttaccc agccgttggt aatccattct tccatttcgc ggcgtgagcc gacgccggag
    16141 gctgcaagca ctttttgcag gcgttcgggt tccatgcgcg acaggtcgct gcggcgttct
    16201 ttcaaatcgc gcgcgtgttc catgattttt tggttgggat tgcgcacgat gagttttttg
    16261 gctttggcgg cgcgctgttt ggggacgttt tgaggtttga tgccgtctga agctttttgt
    16321 ccgtaaggtt gggaagcggt tttgcccgtt tcatctttgg gacgggcttt gcttttgaac
    16381 ggtttggcgg ttttcttggc agacggggct gcgccgtcgc gccattggcg tttgctggtg
    16441 ggctgtttgg acatcatttt tcctaactcg cctgataagg cggttcttct gcggcgtgtg
    16501 cggcaggacg ggttgataaa atgccgtcct cagtcggacg gttcgatatt gtacctgatg
    16561 cggcggtttt gcggatgaaa tcgtcgctgc ggcttgtggc gaggcaaagt ttgtgcaacg
    16621 gttgaaatcg gcttgcgtgc gggggtagaa tctttcaaat aacaagagga gcggtaaatg
    16681 gatttgcaca atattcggga agattacagc aaacgggaat tgtcggaagg cgattgcgcc
    16741 gacaatccga tcgagcagtt cgagcggtgg ttggacgagg cggtacgcgc acaggtcaac
    16801 gagccgacgg cggttaatgt ggcggcggtc gacggacgcg gcaggcctaa tagccgtatg
    16861 gtgctgctga aggaagttaa ttctgaaggt tttgttttct ttacgaatta tcacagccgc
    16921 aaggggcgtt cgctggatgc tcatccattt gcggcgatga cgtttttttg gccggagctg
    16981 gagcgtcagg tgcgcgtgga agggcgtgtt gaaaggctgg cggagaaatt gtcggacgaa
    17041 tattttgaga gccgcccgta tcagagccgt ttgggtgcgt gggcaagcgc gcagagcgaa
    17101 gtgattccga acaaggcggt attggtggca aaggcggcgg cggtcggact caaacatcct
    17161 ttgcacgtgc cgcgtccgcc gcattggggc ggctatattg tgattcccga tttgattgag
    17221 ttttggcagg gcaggccgag caggctgcac gaccgcatcc aataccgttt gctggatggc
    17281 ggctggatac gcgagcggct gtcgccttga acaggaaaaa acatatgccg tctgaaaaca
    17341 ggaatcgggt ttcagacggc attttttcac ggggaaaata cggacgggaa gttttttgtg
    17401 ttccgaacgg aaagcctgaa tcttttttat accggggaat tatgatgcgg acggcgtgaa
    17461 tgcgtcggag aaaaataagt tttccggcag cttatgctgt tgcacaaaca gattttttgc
    17521 ttgttcggtc atggccggag agccgcaggc aaatacttcg tattccgaca ggtcggggtg
    17581 gtcttgtgcc gcgatgtctt gtacgtggcc ttttcttcct tgccagcact ctctggggcg
    17641 ggacaatacg ggggtgaagc aggcgttttt cagacggcat gcgaatcctt gtgcttcttc
    17701 gagggcatac aaatcatcct gatgacgcgc gccccagtag aaatggacga cgcggctgct
    17761 gttttggcgg ataaggtcga gcaggatgct gcggatgggg gcgtagcctg tgccggttgc
    17821 cagcaggatg acgggcttgc cgttgtcttc ctgtaaggta aacgaaccga gcgggccttt
    17881 aacgcggacg atgccttttt ctttgacttt gggttcgctg ccgaaaatca tttccgagca
    17941 gacaccgttt tcgcgcctgc ggatgtgtag ttccaaaatg ccttcttggt cgggcgaatt
    18001 ggcgatggag tagctgcggc tgacgttgcc tggcagcagt aaatcaatgt attgcccagc
    18061 gtagaaggca aacggcgggg ctttgggcag ggcaagtttc aggagggcga catcgtgttt
    18121 gaaaataata ctttcgatgc gtgcgggcag ggtgcggacg ggtagggcat cggctttgta
    18181 gccggggatg ttgatgctga tatcgctttg cgcagtggtg cagcacatca aaatcttgcc
    18241 ttgcgctttt tctgcttcgg ataaagcctg ttccgagtgt ccgcccattt gaatatcgcc
    18301 gctgaccagt tcggctttgc attgcccgca gacaccgttt ttgcaggaat ggggcagatt
    18361 aaggttttgt cgggcggggg cggacaaaac ggtttcaccg tcgttggcgg caaaggtggt
    18421 ttggtcgggc agggtaacgg tgtggttcat ggaatattgt tttttatgct gttttaatgt
    18481 ggtcagactg tgaaaatgcc gtctgaacgt cggatttggg tttcagacgg catcttgctt
    18541 atcaggcaag tttggcttta atcagctctt gaacctgcgc ggggttggct ttgcctttgc
    18601 tggctttcat cacttgtccc acaatcgcat tcagggcttt ttcgttgccg gatttaaact
    18661 gttccacggc tttggcgttg tttgccagca cttcgtccac catcgcttca accgcgccgg
    18721 tatcggtcat ctgttgcaaa ccgtgttttt cgatgatttc ggcaatagtg gcttcgggtt
    18781 ctgcccacat ggcttcaaag gcttttttcg ctaatttgct gcttaatgtg ccgtcggcga
    18841 ttttgcctac cagcgcggcg aggcgcggag cggtaatcgg gctgtcggca agttccatgc
    18901 cttctttgtt cagtgcggcg gcgagttcgc cgttcatcca gttggcagtc ggcttgcctt
    18961 gtccgctctc tttggcggct tcttcaaaat aggcggcctg tgcgcggctt gcggtgagca
    19021 ggcgcgcgtc gtattcggac acgccgtagt ccgccacgaa acgcgctgcc atttctttcg
    19081 gcagctcggg catttctgct ttggcttttt gcatttgagc gtctgaaatg atgacgggca
    19141 gcaaatcggg gtcggggaag tagcggtagt cttgcgcgtc ttctttcagg cgcattacgc
    19201 gggtttcgcc tttttcggga tcaaacagca tcgttgcctg ctgtactttg ccgccgtctt
    19261 ccaaaatctc gatttgcgct tctgcttcgt aattaatcgc ctgctccaag aaacggaagg
    19321 aattgaggtt tttaatctcg cggcgcgtgc cgaattccgc ttgacctttc gggcgaacgg
    19381 atacgttggc atcgacgcgg aacgaacctt ccgccatatt gccgtcgcaa atgtccagcc
    19441 aggttaccaa gctgtgcaag gctttggcgt aggcaacggc ttcggcggcg gagcgcaatt
    19501 ccggttcgga tacgacttcc aataaaggcg tgccggcgcg attcaggtcg ataccggtcg
    19561 cgccgttcaa gccttcatgc acggacttgc ccgcgtcttc ttccatatgg gcgcgggtta
    19621 cgttgatggt tttcacatcg tcgccgacta cgatttccaa tttgccgtgt tcgacaatcg
    19681 gtaagtccaa ctggctgatt tgatagcctt ttggtaagtc gggatagaag tagtttttac
    19741 ggtcgaacac gtttttccgg ttgattttcg catccaaagc caaacccaat ttgatggctt
    19801 tttcaacgac ttctcggttc attaccggca gtacgcccgg caaagcacat tccaccacgc
    19861 tggcgtgcgc gttgggttct gcgccgaatg cggtcgatgc gccgctgaag attttggatt
    19921 tggtgttcaa ttggacgtgg atttccaagc cgattacggt ttcccaggtc ataagagctt
    19981 ctttctctaa aatatcaatt cgatagtctt cgcgtcaggc tgtccggcgg agcggttttt
    20041 ccgtttggat gccgtctgaa atgccgcgtg cttctgtttc agaccgtcct tcatctctgc
    20101 aaccatgttg tccagcattt cataacgctt gcggtacatc gagcgttttt tgctttcgat
    20161 gtcggtaagg tctttgcggg cgggggtttg ggggagatgc cagtagccgt cccgttccaa
    20221 acttgcgccg tattcctgcc agaatgcgtc gtaattcatt ttgacacgct ttttaagttt
    20281 ccagcgcagt ttgacctgat gtttttgtgc aatgcccatt gcgccgtcca agccgagtac
    20341 ggcggcgaaa tattgcaggg cggttaccat cagttgttgg ggacgcaagc cgtgaagttg
    20401 tttggttatg cggcggacgg tgtctttggc ttcttcaccc gccggccctt gtacggaggc
    20461 tgttagcagg tgtgtgccga caaaggcgaa ggtcgccata tacagccggt tgcctgcttc
    20521 gtcgcgcaaa gataaagacc acgcgccttc ttcgacgcag ttgtcgttgc ggttgagcca
    20581 caagctgatg ccgtctgaaa ggtgcgccaa gaccagatgg aagcggtagt cttccatttg
    20641 gtgcaggatg tctgtgccga acagtttttc cgccatgaga aaatctgccg tcatcgcatc
    20701 taaacggcgt ttgcagttaa aacgcctgtc aacaaattcg cgcagcagcg gataggcatt
    20761 ttgcgggcag tggaggaaca gcccctgacg gataggggag gcgttgacaa actgctcgaa
    20821 ggcgtaaatt tctttttttc cgaaaaattt tcgcaatgtg tattttaaat gttgcagccg
    20881 atataaaggc gcatagctgt aaacggtgcg aaaatcgggg aaggtaaagg tgcggttctg
    20941 cttcattcgg gtcgtttgcc gtgccaatcg ctgttgagtt ggatttgatg cgccgcaccg
    21001 aggattttgg cttcggagaa atagttgccg ataagctgta caccgatggg cagtccgccg
    21061 ccgctgaaac ctgcgggcag ggtcaatgcg ggcagtccgg cgaggttgac ggcgatggtg
    21121 tagatatcgc tcaagtaggt ttcaaccggc gaagcatccg ctccgatttt tggggctgca
    21181 gtgggtgcgg tcggcgcgag gatgaggtcg caccgtgcaa atgccgtctg aaaatcatcg
    21241 gcaacgaggc ggcgcagttt ttgggctttg agatagtagg catcgtagta gccgtgcgac
    21301 agtacataag tgccgatcat gatgcggcgt ttgacttcgc tgccgaaacc ttcggcgcgg
    21361 gttttgccgt acatttcttc caaatcggcg aattgggcgg cacggtgtcc gtaacgtacg
    21421 ccgtcgtaac gtgaaaggtt ggtgctggct tctgcggagg cgaggacgta gtaggcgggg
    21481 atggacagct tggtttgcgg cagggaaact tcaatcaatt ccgcgccttg ggctttcagc
    21541 aaatcaatgg tgttttgcaa tgccgtcaga acatcggcgc tgttgccttc gccgaaatat
    21601 tctttgggca ggccgatttt caaacctttg agcggttggt tcaaatcgcg ggtgtagtct
    21661 tctttttcgc gctcgaggct ggtggagtct ttggggtcga aacctgccat cgcgtttaac
    21721 agaatcgcgc agtcttcggc agtttgcgcc atcgggccgg tttgatcgaa gctggaggcg
    21781 taggcgacca taccgaagcg ggaaaccgtg ccgtatgtgg gtttgatgcc ggtaatgccg
    21841 cagtgcgatg cgggttggcg gatagagccg ccggtgtccg aaccgagcgc ggcaggggcg
    21901 aggcgcgcgg caacgacggc ggcggaaccg cctgacgaac cgccggggac gtgctcaaga
    21961 ttccatgggt ttttggctgc accgtagaat gagttttcat tggtcgaacc catagcgaac
    22021 tcatccatat tggtgcggcc gagcgttacc ataccttcgt cgagcaggtt ttggacgacg
    22081 gtggcggtgt agggggagat gaagttgtcg agcattttgg aagcgcacgc gctgcgccag
    22141 ccggtttggc agaaaatatc cttgtaggcg acgggtacgc cggtaagcgc ggaggcgttg
    22201 ccctgcgcga tacgttcgtc ggcggcacgg gcttctgcaa gggttttatc ttggtcgatg
    22261 gtgatatagc cgttgagggc gggatttttt tcggcgatgg cggcaaggta tgcgcttgcc
    22321 agttcgacgg cggaaatctg tttggactgc aacaggacgc ttgcctgttt caatgtgtat
    22381 tgggtcataa atcttccatc cttaaagggc ttcgggcggt attgctgatg ccgtctgaag
    22441 catattcgga ttattcttcg ataacttgcg gtacgatgta cagacggttg cgtacttccg
    22501 gagcacccgc ctgatattcg gcggcgcggt cggtttcggt tacttcgtct tcgcgcaggc
    22561 gcagggcggc ctcgtgcggg tgcgccatcg gttcgatgcc gtctgtgtta atggtttgca
    22621 tctgttcgac catagtgaaa atgtcgttta attcttgaag cgatttttct ttttcttccg
    22681 cagtcaggtg cagtcgggag agtcgggcga ttttgtctac gtcggcaagt gtcagtgcca
    22741 tgaaaagcat ccttgtctga tggtaaagat accgtcgttc gggtatgatg gggcgtattg
    22801 cttgaatttt taattataac ggaaaaatcc gttgcggcgg caatttgcag catcggatgc
    22861 ctgccgcacg ggtttgaagg agggggaaat aatgggattg ggcatggaaa tcggcaagct
    22921 gattgtggct tttttggtgc tgattaatcc gtttagcgcg ttgtcgcttt accttgacct
    22981 gaccaacggg cacagcacga aggagcgcag gaaggtcgcg cggacggccg ccgttgccgt
    23041 gtttgccgtg attgcggtat ttgcgctgat cggcggtacg ctgctgaagg ttttgggcat
    23101 cagcgtcggt tcgtttcagg tcggcggcgg aattttggtg ttgctgattg ccatttctat
    23161 gatgaacggc aacgacaatc ccgccaagca gaatctcggc gcgcagccgg aaacggggca
    23221 ggcgcgcccc gcccgcaatg ccggagcgat tgccgtcgtg cccatcgcca taccgatcac
    23281 catcggcccg ggcggtattt cgaccgtgat tatttacgct tcggcggcta aaacatacgg
    23341 cgacatcgcg ttgattatcg cggccggttt ggtggtcagt gcgatttgtt atgccatttt
    23401 aatcgttgcc gggaaggtca gccgcctgct gggcgcgacg gggctgacga ttttaaaccg
    23461 cattatgggt atgatgctgg cggcggtatc ggtggagatt attgtgtcgg gactgaaaac
    23521 gatattcccg caactggcag gttgattcga tgtttcacag tgtcaatgta tttacgggcg
    23581 aaacgcttta ccgccgcccc gctcaagatt atgcggagtt tgaacggcgg ctggtggatt
    23641 tgaaaatgcg cggcggggcg ttcgcgcaac tgggcgtaac cgaacgtgcc gcgcgcctgc
    23701 aaaaatttgc cgaccgtttg gaggcggaga aagagcgttt tgcggaaatg gtgtgtgagg
    23761 aagtcggacg ctgcttgcac gaatgccgtg cggaaatcgg caagtctatc gaactgatac
    23821 gctattacgc ccgccttgcc cccgaactgc ttgcccacaa aaccatcgcg acgcaggcga
    23881 gtttgagtca ggtgcgcttc gagcctttgg gcgtggtgtt tgccgtcatg ccttggaact
    23941 atcccgtctg gcaggtattg cgtttcgccg ttcccgctat gtgcgcgggc aacgcgtgcg
    24001 ccgtcaaacc cgcgcccagc gtggcacgcg tcagtcaggc actgttcgat ttggcttcag
    24061 acggcattcc tctggcaggc gtgtggctgg atgaggcggg cacgctcaag gcggttgaag
    24121 ataccgatgc aatggcgttt accggttcaa cgcatacggg gcgcatcctt gcggcacacg
    24181 cgggcgcaaa ccttaagaaa accgtgttgg agctcggcgg tagcaacgcc tttatcgtga
    24241 tgcccgatgc tgatttggag cgtgccgccg cagaagcctg ttattcccgt ttccgcgatg
    24301 cggggcaatc gtgtaacgcc gccaagcgca tcatcgtaac cgaagccgca gccgatcgtt
    24361 ttatcacgct gtttcttgcc gaatgcgcta aattgaaaat gggcgacccc aaacatcccg
    24421 atactacgct tgcaccgctg caccgcgaag atttgcggga cagggttcac gggcaggttg
    24481 aagatgccgt ttcaaacggc gcggtatgcc tgaccggcgg aaaaatccca caagggcgag
    24541 gctggtttta tcccgcgacg gttttagaca gggtaaatcc tgcctgccgc gtctggagag
    24601 aagaagtatt cgggccggcc gccctgattt tgcgcgccga aaacgaagaa catgccatcg
    24661 cccttgccaa tgattcaccg ttcggactcg gcgcctgcat ttataccgcc gatactgacc
    24721 gggcatggcg gtttgccgaa aaaatacagg cgggatcggt gtttatcaac cgccatacca
    24781 gcagcgattt gcgcctgcct ttcggcggag tcaaagattc cggttacggg cgcgaattgt
    24841 ccgaattcgg gctgtacgag ttcgtcaacg tcaaaaccta ctggcagaaa tgacaaaccc
    24901 cgtgccgctt cagacggcat cgggtttgcg ctagagccga ttaacggcag tatttgttta
    24961 cggcgttatt gtatttccga atcaaatcat ccttgttttt tgcatttgaa tttcccaccg
    25021 ccttcaggtt catttttgaa atccggcagt tttcttcttt attttgccgt tcggtttcgg
    25081 caattttttt cttttcttcc tcaagttgcc tgtttttctc ggcagtgtcg ggattgtttt
    25141 cctgcgccgc accgtctgtg cgcttccctg cgtcggcttg ggcgggtttg accgccggtt
    25201 tggtttggcg cgtccgcagg ttgaggattt ggctctggtc gggatgaagc tgtttcggca
    25261 catccgaata gctgttgccg ccgccgtcct tccaagtaaa gacggcggct tcggcgggcg
    25321 ggacgggcag gaaagaggcg agggtaaatg tgatgacgga taaagcaaag ttcattaggg
    25381 tttccttaag ggttggctgt cggggcagca tcaggattgg ctcgggcggt gtcttttccg
    25441 gggttcgggt caaagcaaag aagacactcc gccttaatat ggtgctttat atagtggatt
    25501 aaatttaaac cagtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
    25561 cgtcgccttg tcctgattta aatttaatcc actatatatt gggctaaaac agatacccaa
    25621 aaatatcatt ggaatatgct tatgctaccg tatatttcga caaagaaaaa gaaaattgca
    25681 ggcagacgcg tataaaatcc gatggggcgc gtgattctgc ctttggtgtt gaatcggttt
    25741 ttgatcgttt taattgatga aatgccgtct gaacgctttg aaaagggttc ggacggcatt
    25801 ttgttcaaac cggtttattt gcgttgcaat acggcggcga agaagccgtc ggtttggtgt
    25861 cgggcggaat cgaggcgcag gtattttccg gtatccaaat caaccttcag gctttgcagc
    25921 agttcggcgc agttgacgag ttcaaattcg gaatgttcgg acaggaaacg ttcgacttgc
    25981 agctcgtttt cttccggcaa cacgctgcaa gtggcgtaca tcaagcggcc ttgcggtttt
    26041 accagtttgg aggcggcatc gaggatgctg tgctgctgtt ccaaaaggtt ggctacggtc
    26101 tcggcggatt ggcggtattt gagatcggga ttgcggcgta aagtacccaa accggagcag
    26161 ggcgcgtcca ccaacacacg gtcggctttg cctgccagtc gggcgatatg ggtatcgtgt
    26221 tcgctgctga tgcgttcggg gtggatattg gtcagtccgg cgcgggtcat gcgcggtttg
    26281 aggttggcaa ggcgtttttc ggcgatatcg aaggcgtaga ttctgccttt gttggccatt
    26341 tgcgcgccga cagccaaggt tttaccgccg gctccggcac aaaaatcgac aatgatttcg
    26401 ccgcgttttg cgcccaccaa taaggcaagc agctggctgc cttcgtcttg gacttccagc
    26461 gtgccgtcca aaaacagctc gtgtttgtta agcgcaattt tatttttcag gcggatgccc
    26521 caaggtgaat aaggcgtagc ttctgcatcg gcactttcgg cttgcaataa tggcagcact
    26581 ttatcgcgtt tgcctttcag cgtattgacg cggatgtcga gcggggcagg ttggttgatg
    26641 ctgcggccga aagcgaggat ttcttcttcg cgccaatgct gttgcagctg ttccaccagc
    26701 cattgcggca attctgcggc ggtattcagg ccgtctgaaa acttggtttt acgggctttc
    26761 aaattgccga ggaactctgt ttcttcttca tcaagcaggt ctttgatttg gctgatgttg
    26821 gtgcttctgc cgagaaccag tgcggcgaga gcggctttgc gcggctgcgc gtgcggacgg
    26881 cgcagggcag tgctgatttt ttgatagtgt cgcagcgcgg caaaggcggt ttcggcgatt
    26941 tcgtggcggt cttggcggcc aagctttttg tgttcgcgga aataggcgga gaggacggca
    27001 tcggaaggct gtttgaaagt cagcatttca gccaaaactt tggcggtatg gtcgagttgt
    27061 gcggcgttca taatgtaatg ggttctctta aagttggtaa atggtttcag acggccttca
    27121 tgatgcgttc gatttcgtgc caaaagccgt ccggccgtaa ttccaaaacg gcaatcaagc
    27181 cttggtcgat acggcggatt tcggttgcgt ttaatatttc ggtttcggca acacgcgcag
    27241 gcgcaagata ggccatgcgg tcgagcagat ggtagcgggc ttcttggcgt tcaaacccgt
    27301 attccaccca atcttgaata tagatgccgc gccagccgta tgggttaagc ggcggctcaa
    27361 aagcatggaa actttgtgga aaaaatccga tgccgcgcac gatggaaacg ggttttacgt
    27421 caagcggcag accgagttgg gcggtcagtt tggcggcttt tcccaacagc gtgtctgtgg
    27481 ggtcgagtcc gcgcatgtct tcgggtctgc ccgtgccgcc gccgtaatat ttgcaggtca
    27541 gctcgatatg gtagggtttg ccgttaagcc ttgccacaaa atccgccgcg ccttgcgtat
    27601 tgccgtccga accggaaacc gtgaggttgt gcgcgagcag ttcggcgtgc ggcgcgttgg
    27661 caaaccaaaa tgccgacagt tcttcggcgt aaatgccgag gcggtgtccg aatggggcgt
    27721 gtcgggcgag gtgttccgtc agcggcgcgg gatcggcatc caatgccaaa aggtaacgga
    27781 aaccgcgttc ccccagtagt tcgcgcacgc ccaattcgca accgctttgc cacaaaggcg
    27841 gcgcagtcag cagcgaggct aaatcccgca cggtagggtc ggtcagtttc caccacaggg
    27901 catcgagggc gtagttcatg gttttcctgt ttctgtattc tgatttttcc tgaaatgccg
    27961 tctgaaaaac ggattcagac ggcatgcgct taggattgcc cgtccttcag acgctgttgc
    28021 agcgtattga aaaaaccgcg caggatatcg atgtcttcgg tttgcgtgtt ggcgcgtccg
    28081 aacaggctct gcatacggcg catcagacgc tcgccgttgc ggcggttgaa aaagccgatg
    28141 tcgttcatca cgctttccat gtgggcgacc atgcctttga tttgctcgtg ggtcgcagcg
    28201 tggtcttctt gttgaagatg ggtcatgggc gaaccagttt ggctgaagat ttcgtagcac
    28261 acgacctgta ctgcttgggc gaggttgagc gagaaataat cggggttgcc gttgatggtc
    28321 atcagtcggt tgcaggcttg gacttcttcg atgctcaagc cgaaagtctc gttgccgaaa
    28381 accagtgcca ctttctcgcc tcggttggcg gtctgcagta attcggatac caaatcgcgc
    28441 ggggtttgca gcggcgcagt aatttcgcgg cggcggctgg tcagggcgca ggcgatggtg
    28501 gtgtcggcaa gggcttcgtc caaagaagcg gcaatggtgg cattttccaa aacatctgcc
    28561 gcgccggaag cgaggatgaa gctttcttcc ggtaatttaa acgattgagg atgctccgga
    28621 ttgaacacgg gcggattctc cgtcatcggc gtagccatca gattgggggc gacgatggtc
    28681 agtttgtgca gacccatcgt tttcatcgcg cgcgcggcgg aaccgatatt ggcggggtgg
    28741 ctggtgcggg tcagaacgat gcggatgttg ttcagataag cgggcagggc gggtttgaga
    28801 gcagtcatgt tcggttttcg gtcggaatgg aatagggtat aatgcgccgt ctttttcgtt
    28861 cggacggtca aatgccgtct gaaatgttct ttaacaacgt cggaaattta acgttgcgcc
    28921 tgaagcagac acgcgtcggg cggtatttta cccgatttgg caggatttac cccaaccaaa
    28981 taaactccga ttttgccgct ccgaaggact gcgcgtccga accggcggta tgccaccctt
    29041 aaggaaatct gatgaatccg tttttgaata cagcctttaa agccgcccgc cgtgccggtc
    29101 agatgatgat acgcgccgcg ggcaatctcg atgccgtcaa aaccgacagc aaagcattca
    29161 acgattttgt ttccgatgtt gaccgcaatt ccgaaatcat cctggttgag gctttgaaag
    29221 aagcctatcc gcaccacaaa atcacttgtg aagaaagcgg ctcccacggc aaagccgcgg
    29281 ccgagtacga atggattatc gatccgctcg acggcacgac caatttcctt cacggccatc
    29341 ctcaatacgc tatctctatg gcgctcctgc acaaaggcgt gttgcaagaa gctttggtgt
    29401 acgcacccga acgcaacgac gtatatatgg catcccgagg caaaggcgcg ttgctcaatg
    29461 accgccgcat ccgcgtttcc aaccgcatcg aattgaaccg ctgcctgatc ggtaccggct
    29521 ttcctgttgt cgatcaaagc atgatggaca agtatttggc gattttgaaa gatttcttgg
    29581 caaaaaccgc cggcggccgt cgtgaaggcg cggcttcttt ggatttgtgt gctgtggcga
    29641 ctagtcgttt ggacggtttc ttcgagttta acctcaaacc ttgggatatt gccgcaggcg
    29701 cattgattgt tcaggaagcg ggcggtatcg taaccgatat gacgggtgaa gacggctggc
    29761 tggaaagcgg cgatattgtt gccgccaatc ccaaagtgct ggcgcaaatg ttaaaaatca
    29821 tttccgcaca cgtttaaatt tcagcccgct cccgggaatg cgggaaaacg ggcgggtatg
    29881 ccgtctgaag tatcgcgccg atatttcaga cggcattttt actggcagcg ggggcaggaa
    29941 ggtggaaacg gctaaatgcc cgaaactgcc gaagatggaa acccatatct gattaaacgg
    30001 agaaccctgt acgatttgtt tatagtggat taacaaaaac cagtacggtg ttgcctcgcc
    30061 ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatgg gcaaagccaa
    30121 cgcctttacc gcgccgatta ccgtcgtcaa ctacgacgaa caagccctca acaacaccga
    30181 agcgcgtact ttggtggatg ccgtagcgaa aaaagacgct tccgtttggc agttcggcgg
    30241 agaaagcaac acgctgaccg gactgtattt cagaggttat cagcttgatg cgcgccaatt
    30301 cagcgtcaac ggtttggcag gtatgtacgg cacgcaaggc acggccagcg tgcaagtcgg
    30361 ctccgcacaa ctgattaaag gcgcgtccac cgccgtaaac ggcatgaacc ctgaaggtgc
    30421 ggtatccggt tccgtcaata tcgaaaccaa aaaagccgcc gatgaaggca accgcaaaat
    30481 cggtttgggc cggtttagca acaaccgcgc ccaaggtacg ttcgacttgg gtcaacgctt
    30541 cggcgaaaac aaagcattcg gcgtacgcgc caacggcaaa ctgcgccacg gcgacactcc
    30601 gcgccacggt tacagcgaag acaacaaaga atttgcggta aatgccgact atcgcggcga
    30661 aacactgcgc gtagcgttcg attccatcta cgcaaaacgc aaaaccaacg gcggtcgcgc
    30721 gcgtatgcag gacattcaaa acgccggcgg acgcttgttt ggcgcgcccg atggtaaaac
    30781 caacctgctg ccttcttgga actggcaaaa taccgccggc caaaccaata tgcttacgtt
    30841 tgaatgggat gcgtttgata atgcacagat tacaggcggt atcggctaca acaaggcacg
    30901 ctattacggc acgctgattt ccccgaccgt atgtttgaat gctaccagca cttgtaccga
    30961 tatgcaaaac acaaacgcca gcggcaaagt aacggcacgc aactatgact acaataccgg
    31021 tactgcgcgc ctgaccgacc aatatttccg cacattgagt atgaacctga ccgcacgcgg
    31081 cgaatttgaa accagcccgg taacacataa ctggagtacg gctttcgacc gcgttatccg
    31141 ccagcggaaa accattcgcg gaacggcagc cggcgctggc aaggtagaag tgaaggcaaa
    31201 cgaaaatatt gcgaatcaat tggcatcgtt caaagccgac tatcccaatt cttgggagaa
    31261 ctctgccaac ttggatgcca atatcaaagt caacagcctg gctttgtccg acaccttggg
    31321 cttcgtggat aacaaatacc gcctgacttt gggcggacgt ttccaagcgg ttgaatacac
    31381 caataagaaa aaatcacaga gcggcgatgc caaacgcttc agcccgatgc tgatggcggc
    31441 gtgggtgccg caacctgatt tggttgtgta cggcaactat atggaagact tggagcctgc
    31501 cgatatcaaa accgatgatt ccggcgaaac gacaatggcg aaaccgcgtg tcagccgtca
    31561 gtttgaagtg ggcgtgcgta aaaactgggg caatttcgta accacgttaa acgcgttcca
    31621 aatcaaacgc ccgggctact ggcgcggtca tacaactacc aagacaacaa aaggggtaac
    31681 aacaacgctg acctatggca acaattccga tttcgcccgc tataaggcgc aaggcggcgc
    31741 ggccggcgac gagcaaggta tggaacgcaa tcgcggtatt gagttcaaca cctacgcgaa
    31801 cttgctcaac aatactttgc gtccgacttt gggtctgatg tatctgcaat cgaccgtgaa
    31861 agagtatcca aattcacgcg atatgctggt taacggcgta caagtcgcca atccgcgcgt
    31921 aattgccaag gcaggtttgg aatgggatac accgtttgcc aaaggcttga ctttaaacag
    31981 caatgtttcg tatttcggca agtcttacca agacacgcaa aaacaatacg ctttcccgtc
    32041 ttacactttg gttgacgtag gcgcgcgcta caaaaccaag ctcggcaaaa atacgttgac
    32101 cgtcagcagc tcggtggaaa acctgttcaa caaaaactac tggcaggtac agcgcggcca
    32161 atacgaccgc agcttcgccg tcgtcggtat gccgcgtact tactggttga aagcggaatt
    32221 ggatttctga tttctccgca tccggaaaat gccgtccgaa caggtttcag acggcatttc
    32281 ttttaaagaa atatggcggg atatgcttaa ataatgctat tctagcgtct ttgaaacctg
    32341 cttgtttgaa gtgatgaaaa aacctttgat ttcggttgcg gcagcattgc tcggcgttgc
    32401 tttgggtaca ccttattatt tgggtgtcaa agccgaagaa agcctgacgc agcagcaaaa
    32461 aatattgcag gaaacgggct tcttgaccgt cgaatcgcac caatatgagc gcggctggtt
    32521 tacctctatg gaaacgacgg tcatccgtct gaaacccgag ttgctgaata atgcccgaaa
    32581 atacctgccg gataacctga aaacagtgtt ggaacagccg gttacgctgg ttaaccatat
    32641 cacgcacggc ccttttgccg gcggatttgg cacgcaggcg cacattgaaa ccgagttcaa
    32701 atacgcgcct gaaacggaaa aagttttgga acgctttttt gggaaacaag ctccggtttc
    32761 ccttgccaat accgtttatt ttaacggcag cggtaaaatg gaagtcagtg ttcccgcctt
    32821 cgattatgaa gagctgtcgg gcatcaggct gcattgggaa ggattgacgg gaaaaacggt
    32881 ttatcaaaaa ggtttcaaaa gctaccgcaa cagctatgat gcgcccttgt tcaaaatcaa
    32941 gctggcagac aaaggcgatg ccgcgtttga aaaagcgcat ttcgattcgg aaacttcaga
    33001 cggtatcaat ccgcttgctt tgggcagcag caatctgacc ttggaaaaat tctccctaga
    33061 atggaaagag ggtgtcgatt acaacgtcaa gttaaacgaa ctggtcaatc ttgttaccga
    33121 tttgcagatt ggcgcgttta tcaatcccaa cggcagcatc gcaccttcca aaatcgaagt
    33181 cggcaaactg gctttttcaa ccaagaccgg ggaatcaggc tcatttatca acagtgaagg
    33241 gcagttccgt tttgacacgc tggtgtacgg caatgaaaaa tacggcccgc tggacatcca
    33301 tatcgccgcc gaacatctcg atgcttctgc cttaaccgta ttgaaacgta agtttgcaca
    33361 aatttccgcc aaaaaaatga ccgaggaaca aatccgcaat gatttgattg ccgccgtcaa
    33421 aggcgaggct tccggactgt tcaccaacaa tcccgtattg gacattaaaa cttttcgttt
    33481 cacacagccg tcgggaaaaa tcgatgtggg tggaaaaatc atgtttaaag acatgaagaa
    33541 ggaagatttg aaccaattgg gtttgatgct gaagaaaacc gaagccgaca tcagaatgag
    33601 tattccccaa aaaatgctgg aagacttggc ggtcagtcag gcaggcaata ttttcagcgt
    33661 caatgccgaa gatgaggcgg aaggcagggc aagtcttgac gacatcaacg agaccttgcg
    33721 cctgatggtg gacagtacgg ttcagagtat ggcaagggaa aaatatctga ctttgaacgg
    33781 cgaccagatt gatactgcca tttctctgaa aaacaatcag ttgaaattga acggtaaaac
    33841 gttgcaaaac gaaccggagc cggattttga tgaaggcggt atggtttcag agccgcagca
    33901 gtaatgggtc aaatgccgtc tgaaatgttc aggcggcatt ttgttgtatt ggaaactgcc
    33961 gttttcaacc attcctttag ataagccgtg tcggattc
//
LOCUS       DAWFJW010000009        33706 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFJW010000009 DAWFJW010000000
VERSION     DAWFJW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.009"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(58..1368)
                     /locus_tag="WGR19_000348"
     CDS             complement(58..1368)
                     /locus_tag="WGR19_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0797945.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WGR19_000349"
     CDS             complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WGR19_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0797946.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WGR19_000350"
     CDS             complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WGR19_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0797947.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVTSCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(4134..4862)
                     /locus_tag="WGR19_000351"
     CDS             complement(4134..4862)
                     /locus_tag="WGR19_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0797948.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(4852..5766)
                     /locus_tag="WGR19_000352"
     CDS             complement(4852..5766)
                     /locus_tag="WGR19_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0797949.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(5880..7289)
                     /locus_tag="WGR19_000353"
     CDS             complement(5880..7289)
                     /locus_tag="WGR19_000353"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0797950.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(7444..8511)
                     /gene="murG"
                     /locus_tag="WGR19_000354"
     CDS             complement(7444..8511)
                     /gene="murG"
                     /locus_tag="WGR19_000354"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0797951.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADIAKLPAPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8515..9840)
                     /locus_tag="WGR19_000355"
     CDS             complement(8515..9840)
                     /locus_tag="WGR19_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0797952.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9966..11303)
                     /locus_tag="WGR19_000356"
     CDS             complement(9966..11303)
                     /locus_tag="WGR19_000356"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0797953.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHQADFWLERETGRLKQGDEDLIATQDIPLQGLHN
                     AANVMAAVALCEAIGLPRNELLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDTLAGKVKGVFLIGVDAPQIHRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(11388..11660)
                     /locus_tag="WGR19_000357"
     CDS             complement(11388..11660)
                     /locus_tag="WGR19_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0797954.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDRALAQEWYGKACQNRDQDGCGHYQRLKAGY"
     gene            complement(11775..12857)
                     /locus_tag="WGR19_000358"
     CDS             complement(11775..12857)
                     /locus_tag="WGR19_000358"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0797955.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13104..13280)
                     /locus_tag="WGR19_000359"
     CDS             complement(13104..13280)
                     /locus_tag="WGR19_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797956.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(13283..14641)
                     /locus_tag="WGR19_000360"
     CDS             complement(13283..14641)
                     /locus_tag="WGR19_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0797957.1"
                     /translation="MKPLDLNFICQALKLPMPSESKLVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCASLKDALKVDDTLAALQTLAKAWRENVDPF
                     VFGITGSVGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14766..16412)
                     /locus_tag="WGR19_000361"
     CDS             complement(14766..16412)
                     /locus_tag="WGR19_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0797958.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYVAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(16435..17913)
                     /locus_tag="WGR19_000362"
     CDS             complement(16435..17913)
                     /locus_tag="WGR19_000362"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0797959.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDLG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPNPERVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(17938..19683)
                     /locus_tag="WGR19_000363"
     CDS             complement(17938..19683)
                     /locus_tag="WGR19_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0797960.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(19744..20007)
                     /gene="ftsL"
                     /locus_tag="WGR19_000364"
     CDS             complement(19744..20007)
                     /gene="ftsL"
                     /locus_tag="WGR19_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0797961.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(20004..20990)
                     /gene="rsmH"
                     /locus_tag="WGR19_000365"
     CDS             complement(20004..20990)
                     /gene="rsmH"
                     /locus_tag="WGR19_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0797962.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(20987..21442)
                     /gene="mraZ"
                     /locus_tag="WGR19_000366"
     CDS             complement(20987..21442)
                     /gene="mraZ"
                     /locus_tag="WGR19_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0797963.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            21721..22938
                     /locus_tag="WGR19_000367"
     CDS             21721..22938
                     /locus_tag="WGR19_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0797964.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEEIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFSFGDICHKL"
     gene            23070..23146
                     /locus_tag="WGR19_000368"
     tRNA            23070..23146
                     /locus_tag="WGR19_000368"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23104..23106,aa:Met,seq:cat)
     gene            23154..23229
                     /locus_tag="WGR19_000369"
     tRNA            23154..23229
                     /locus_tag="WGR19_000369"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23187..23189,aa:Ala,seq:ggc)
     gene            complement(23511..24332)
                     /locus_tag="WGR19_000370"
     CDS             complement(23511..24332)
                     /locus_tag="WGR19_000370"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0797965.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGLFILWVEKRQSWAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(24365..25009)
                     /locus_tag="WGR19_000371"
     CDS             complement(24365..25009)
                     /locus_tag="WGR19_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0797966.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKQFDGKKLMRAYDSPEAAAAASKMQK
                     LTEQYGIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(25012..25842)
                     /gene="ftsN"
                     /locus_tag="WGR19_000372"
     CDS             complement(25012..25842)
                     /gene="ftsN"
                     /locus_tag="WGR19_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0797967.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALYEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEEKKAAK
                     EKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRRSAEGQ
                     RAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIRSIESK
                     "
     gene            complement(25948..27444)
                     /locus_tag="WGR19_000373"
     CDS             complement(25948..27444)
                     /locus_tag="WGR19_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0797968.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLVQTDCQVPQMPSENTKLLDLVDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(27458..27778)
                     /locus_tag="WGR19_000374"
     CDS             complement(27458..27778)
                     /locus_tag="WGR19_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0797969.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            28261..29787
                     /gene="putP"
                     /locus_tag="WGR19_000375"
     CDS             28261..29787
                     /gene="putP"
                     /locus_tag="WGR19_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0797970.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFTGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWI
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            30038..33643
                     /gene="putA"
                     /locus_tag="WGR19_000376"
     CDS             30038..33643
                     /gene="putA"
                     /locus_tag="WGR19_000376"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0797971.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         7932 a         9706 c         8351 g         7717 t
ORIGIN      
        1 cggcaaacaa aatgccgtct gaaaaccttt cagacggcat ttttaatatc gaatcaatca
       61 gcccaaatac cagaatttca gtgcccacaa tacggcaaca atccatacca taggcggaac
      121 gtctttggtg cggcggcata aaagtttaac cacggcatag ctgatgaagc cgaaggcgat
      181 gccgtctgca atcgaatagg taaacggcat gaagacaatg gtcaggaatg cgggtgcggc
      241 ttccgtcata tcgtcccaat caatatccct cgcactgcgg agcatctgcg tgccgacata
      301 aagcagggcg ggcgcggtgg caaaagcggg aacacttttc gccaaaggtg aaaacatcag
      361 gcaggcgagc atcaatacgc cgacggtaac cgccgtcagg ccggtccgtc cgcctgccga
      421 tacgcccgcc gcgctttcca cataaggcgt ggtggaagaa gtacccaaag ccgcacctgc
      481 cacaatggcg gtagagtctg caagcagtgc gcgtttcagg cggggcagct taccgtccac
      541 cagcagcccg gcacggtggg atatgccgac cagcgttccg gtactgtcaa atagatcgac
      601 caagaagaag acgaaaatca cactgaccat gctgacggta aacaggcctt caaaatccat
      661 ctgcataaaa gtcggcgcaa tgctcggtac ttcgccgatg atgccgtgaa attcattcaa
      721 acccatcagg ctggcaatga cggtaatggt caagatggtg atgatgattg cgccttgaac
      781 gcggaaatgt cccaatacga ccaccatagc aaaaccgaac agtgccaaca acgcggacgg
      841 ctgatgaata tcgcccaaac cgaccaaggt tgccggattg gcaacgataa tgcctgcgcc
      901 tttcagggaa atcagtgcca aaaacaaacc gataccggca gcaatcgaca ttttcaaacc
      961 cataggcagt gcgttgacca gcatttccct gactttaaaa aagctgaaca ggatgaaaat
     1021 cagaccggag atgaacaccg cacccaacgc aacctgccaa ggcacgccca tacccttaac
     1081 gacggcaaag gtgaaatagg cattcagccc catccccggt gcgagtgcaa tcggatagtt
     1141 gccgacaaaa cccataacaa aacagccgat ggcagacgcg atacaggtag cgacgaatac
     1201 cgcccccata tccatgccgg tctcgcccaa aatcagaggg ttgacgataa cgatgtagca
     1261 catcgtcaaa aaagttgtca aacccgccat caactcggta cgcaccgtcg taccgtttgc
     1321 cttcagctta aaaatcccgt ccaacagtgt ttgttttgaa gtgtccatat ctgaaagtct
     1381 gtatcaagtg gaaaaatctg aatccgccga ccgcaaaccg tacgaaattg caacatcttg
     1441 aaaaacatac ctcccaaacc ggacaaaccg attcgggagg cagcaggttt acgggcaaac
     1501 agcacattat ttgtctgaat tgtgttgacg acgcaaaatc gcagggattt caaagtcgtc
     1561 aagtacggac tgattgtcga aatccgcagc ggtaaggttc atcgtgcgga taccgcgatt
     1621 ggtgcggatc ataccttcga cattgtggct ttgctcctgt ttggacgggg caaccgcttc
     1681 tacctccctt gccggaacaa aatcgaccgc gcctttttct ttcagaccgg tagcgataat
     1741 ggtaatccgg atggcatctt cgctcatggt ctcgtcttca gcagcaccga atttgcattc
     1801 caaatcggga tgcgcgcttt ggttgacgat tttcatgact tcggacaact cggacatttt
     1861 caagcaaccc ggagcagtcg taatattgac cagcacaccg cgcgctccgt ccaaggttac
     1921 atcgtccagc agcggactgg aaatggcctg gtcggtcgcc atacgcgcac ggtcgatacc
     1981 ttgggcataa cccgaaccca tcatagcgat accgcggttg ctcatcacgg ttttcacgtc
     2041 ggcaaagtcg aggttgatga tttcgctcgg gcaagttacc acttcggaaa tgcctgcgac
     2101 cgcatcgcgc aatacattgt cggcggcacg gaaggcttcg cgcatcgtta cgtcttcacc
     2161 caatgcagtc atcagtttgt cgttcgggat gataatcagc gaatcgacgt gttctttcaa
     2221 ctgttccaac cctgcctgtg cgacatggac gcgcttacct tcatatgcga acggtcgggt
     2281 aaccacggca acggtcagaa tgcccaaaga cttggcaatc tcagcaacaa ccggcgcgga
     2341 accggtaccg gtaccgccgc ccataccggt cgtgataaac agcatattcg caccgcgaat
     2401 ggcttcttca atggcttccc ggtcttcctg ggctgccgca cggccgatat cgggattcgc
     2461 gcccgcgccc aaaccgcgtg tcagattcgt acccaactgg attctcttcg ccgcatggtt
     2521 ttttgccaga gactgcgcat ccgtattggc actgataaac tccacaccgc gcacattgtt
     2581 ggcaaccatg ttattgattg cattgcaacc gccgccgccc aagccgatta ctttaatcac
     2641 cgcagggctg actgccgatt ctgccacgtc gtaaacaaat tccattcaaa aactcctgct
     2701 cgccccattc agaggacggt ttaaataaat tattattcat tatataagaa gtatcttgct
     2761 gctggcaaaa tacttctcac ctgtcaaacg gcaatccacc tgttcagaag ctgttttcaa
     2821 tccaccgttt caatcttgcc aacaaaccgc cgcccccttc cctctcttgc actgcaccgt
     2881 tttccggctg cggcaagttt ccttccagct tgcatgctgc atgaagcagc ccgatggcgg
     2941 tagaaaagcg cggtgtgcgg acgcggtcgg acaaaccgcc catttcttgg ggcgcaccgg
     3001 tgcgtacagg caaatcgaag attttttcgg caaattccac aatcccggtc atcatggaca
     3061 caccgccggt cagaacgata cccgcattca gcacttcttt ggggaaaccc gatttttgca
     3121 gctcgcccag cactacgcca aaaatctcct gaatacgtgc actgatgatt gctgccaaaa
     3181 ccttactgga aacctgacgc gatgtccggt cacccacgcc cggaacttca atcatctcac
     3241 ccaagccttc cgtatcgcat gatgtcacgc cataatggat tttaatgtac tcggcggcat
     3301 cgagaggtgt tctcaacgat ttggacaaat ctttggtaat cagattacca ccggccggaa
     3361 tgacggacgt atggcggatg gcaccgttca tataaacggc aatatcggtc gttccgccac
     3421 caatgtcgat gacgcatacg ccgaggtctt tttcatcttc agtcagcacc gcctgcccgc
     3481 ttgccaacgg ctgaagcatg atttgatcgc ttttcaagcc gcaccgctcg atacattttt
     3541 ggacattctg cactgccgta cttgcaccgg taatgatgtg cacccgcgta tccagacgca
     3601 caccgctcat accgatgggc tccctcacgc caagttgggt gtcgataatg tagtcttgaa
     3661 ccacggcatc gagaattttt tgatcgggcg ggatattgat tgcctttgcc gtttcaatgg
     3721 cgcggtcgat gtctgcctgc gtgacttccc cgtctttaat tttaaccaca ccttgcgaat
     3781 tgagactgcg gatgtggttg cctgcgatac ctgtggtaac gtgggtaatt ttggtatccg
     3841 ccatcagctc ggcatcattg accgcctgcc tgatggcttg gacggtggca tcgatattgg
     3901 ttaccatgcc cgcgcgcaag ccccgtgaag gagcctgccc caaaccgacg atgttgattt
     3961 tgtcgtcatc ttgaacttcc ccgatcagtg cgaggacttt agacgtaccg atatccagta
     4021 cgctgatgta tctttgctgc tgttccattg ttcgtctgct cttaaaactg attgaaattt
     4081 gcgtcgcacc gtttcagacg gcacggtcgt aatctgtccg atacctgttc ccactattct
     4141 tcggattctt tttcgggtaa accgtcggaa gcatagcgga ctgaaaatcc gtccttatac
     4201 ctcatatcca cataggataa ccgattttta tttttacgca acagatgctg ccacgcttcg
     4261 gtaaaaagcc ggaggcgttt catctcgttt tcccgtccga gcctgacggt gatgccgttg
     4321 tccaaaacga caatccacgc cgaacgtgcc gtataggtca tctctttgat gcccaaaccc
     4381 tgttttgcca aaacagtcga aaattcgtca taacggcgga gcatttcggc agacgttcct
     4441 tccgcgcctc tgaataccgg cattccgggt ctgtccaagc gggcttcaaa aacattgcct
     4501 tcgccgtcca ccaaggcatg gtcgccccaa cgcgcgaccg gcttgcgctc ggtcaggacg
     4561 acctcaaccg tgtcgggaaa acggcggcgc accatgaccg acgcaatcca cggataccgg
     4621 cggtaggcct cctgtgcgcc attgatgtcc gtcctcaaaa tattcccatg gatgtattct
     4681 ttcgccaaac tgcccaatgc cttcttatcg gaataaacca ggttgccctt cagcgacacc
     4741 tgcttgacgg gcagatgatt cgaattgtaa aaccaaacca gcccggacgc agcaagcagc
     4801 atcgccatca tgacaagcag ccagcgcgtc agccgttcca tcgcttcggc attatcccac
     4861 atgtgcggtc ttcaaaattt caatacataa atcggcaaaa cccacgcccg taacggcagc
     4921 ggatttcggt actaaactat ggctcgtcat accgggcagg gtgttgattt ccaacagata
     4981 gagtttgccg tcggtatctt tgaggaaatc gacgcgcacg cagccttccg caccgattgc
     5041 ctgcgcgccg cgaaccgcca gttcgcgcat caggctttct tcggcttcgg tcaaatcttc
     5101 cgaaggacat tgataaatgg tgtcgtcgcg gttgtacttg gcttcgtagt cgtaaaactc
     5161 ggttgcggga atgatgtgta tgccgggcag gcctttgccg ttcaacacag ggcaggaata
     5221 ttcgccgccg ccgataaacc gttcggcaat gatttcgccc tgaaggtgtt tcaattcttc
     5281 gtaaacgctt ttcagacggc cttttccttt gacttttacc acgcctacgc tgctgccttc
     5341 ggccgccggt ttcacaaaca tcggcaggcc caatttttct tcaacggcat cgaaatcagt
     5401 gtcgtcgtgc aggacggcga actcgggaac gggcagtccc aatgcctgcc aaatcagttt
     5461 gcagcggtat ttgtccatgc cgatggcgga tgcggcgaca ccgctgccgg tatagggaat
     5521 gcccaacagt tccaatgcac cctgaaccgc cccgtcttcg ccgtaagtac cgtgaaggat
     5581 gttgaatgcc gtctgaaaac cttgtgcctt caattcagac aatggggttt ctttaggatc
     5641 gaaagcgtat gcgtctatgc ctttgctttt taaagcattc aaaatggcgg tgccgctgtc
     5701 cagcgagatt tctcgttcgc tggaaaaacc gcccatcaat acggccactt tgccaaaatt
     5761 ctgcattgtt ttttgttctt tcctgattgc tttatgcttg ttgccagagg tcgtctgaaa
     5821 cctgatttgc ggtttcagac gacctttata tgatgttccg tctgtcaggc gggtgtgcct
     5881 caaatctgtt tcgacaattc cagcagcgcg gcggggacgc ggttgatgct tcccgcgccc
     5941 atattcaaca cgatgtcgcc gtcctgcaaa acgttcaaca gcatttcggg cagatcggca
     6001 acgttttcgc agtaaatcgg ctcaagtttg cccaatacgc ggatggcgcg ggcaagagcg
     6061 cgggaatcgg cggcggcaat cggctcttca ccggcggcat aaacttcggt cagcaccagc
     6121 gcgtcaacgg tattgaggac tttggtaaag tcttcaaaca aatcgcgcgt gcgggtatag
     6181 cggtgcggct ggaaggcgag taccaaacgt ttttccggat acgcgccgcg tgcggcggca
     6241 agggtcgccg ccatttcgac ggggtggtgt ccgtagtcgt ccaccaagag cgcggtcccg
     6301 ccgtttggca acttgatgtc gccgtatttt tggaagcggc ggccgacgcc ttcaaagccg
     6361 agcaagcctt tttggatcgc ttcaaccgat gcgccgactt ccagcgccac gccgatggct
     6421 gccaatgcgt tcagcacgtt gtgtctgccg ggcatattca gcacgacttc aaacgacccc
     6481 tgctcatgtc ctttcatttg aacatggacg gtgaatttca tttgcgcgcc gacgttttcg
     6541 atgtcggtgg cgtagatgtc ggcggtatcg tccaaaccgt aagtagcata aggtttgctc
     6601 actttgggca aaatcgcgcg gacgtgttcg ctgtcaatac acaaaaaggc tttgccgtag
     6661 aagggcatac ggtggatgaa atcgataaac gcctgatgca gtttttcgac gctgtgcccg
     6721 taggtatcca tatggtcttc gtcgatattg gtaacgacgg acataatcgg tgtcaggtgc
     6781 agaaaggatg catccgactc gtcggcttcg gcaacgatgt attcgccttt gcccaagcgg
     6841 gcgttggtgc ctgcggcgtt gagtttgccg ccgataacga aagtcgggtc aagtcctgcc
     6901 gcgccgagga tggaggcggt caggctggtg gtcgtggttt tgccgtgcgt gccggcaatg
     6961 gcgatgccgt cacggaagcg catcaactcc gccaacatca gggcgcgcgg aataacggga
     7021 atttgctgct ccaacgcagc gacaacttcg ggattttctt ttttgacggc ggtagaggta
     7081 acgacgacat ccgcaccgtt aacgtgttcg gcggtatggc cgggataaac ttgaatgccc
     7141 aggctgccca aatgctcggt agcggcatta cgcgcctgat ccgaaccgga aactttaaaa
     7201 cccaaattgt gcaagacttc ggcgatgccg ctcatgccga cgccgccgat accgacaaaa
     7261 tggatgttgg tcactcgatt tttcatcata atgttgcgtt ccggtggatt ttcgatgcgt
     7321 aaaggcgtta ttttaaaggg ctgaccgttt gcgcgccata gttttctgac aaatatatag
     7381 cggattgaaa taaaaaacat ccatgctgtc tggacggcat ttcagacggc atggttcggc
     7441 agtttacgcc gcacacgcaa tcgcggcttc ggcaacgtcg tccgcgctgt gcggcaatgc
     7501 caaagtacgg gcgttttccg cccatttgag acatttttcg cggttcaggc tgccgaggat
     7561 ttcggcgagt ttttccgccg tcaactgggt ttgcggcaac agcaatcccg cctccgcctg
     7621 caccataaaa cgcgcgttgg cggtttgatg gtcatcaacg gcgtgaggat acggcactaa
     7681 caacgcaccc aatcccgccg ccgtcaactc ggcaatcgtc agcgcgccgg cacggcaaat
     7741 caccaaatcg gcatcgcggt aggcggacac catatcggta ataaattcca cgcattcggc
     7801 tttcacgccc agcgcgtcgt aatccgcctg caagctgccc agcttgcccc gtcccgattg
     7861 gtggtacatc tgcggacgcg cattgtcggg cagcaaagcc aatgcctgcg gtacggtttt
     7921 gttcaaaacg tccgcgccca aactgccgcc gaccaccaaa attttcagac ggccttcacg
     7981 cccttggaag cgttcggcag gcgcgggcag cttggcaata tcggcgcgga cgggattgcc
     8041 gaccaagccg ccttcgtggc tgaacgcttt cggaaaagcg tacaacaccc gcttcgccca
     8101 gcgcgacagg tggcggttgg acaaacctgc cacggcgttt tgctcgtgaa tcacaatcgg
     8161 cacgcccaag agtttcgctg ccagaccgcc gggaaaggta acgaagccgc cgaagccgat
     8221 gacgcactcg acacggtgtt tgcggataat ccgctgcgct tcgcggacgg tttgatacaa
     8281 agtaaacggc aacatcagct tgcgcttgat gccgttgccg cgcacgccct taatcgccag
     8341 cgtttccaag cgtatgccgt attgcggcac gatacgctct tccatcgaat ccttgctgcc
     8401 cagccaaatc acatgatggc cgcgcgcgcg caatgaatcc gccaccgcca gcgcggggaa
     8461 aatatgtccg cccgttccgc ccgccatcag cataaaggtt ttaccgccca tgatttactc
     8521 cacccgataa ccgcgcattt tccggcggtt ttcataatct atacgcaaca gcagcatcat
     8581 gctgatcagc atgaaaaaga ctgacgaacc gccataggac atcaacggca gcgtcagacc
     8641 tttggtcggc aaagcaccga tgttcacacc gatattgaag aaactttgga taccgatcca
     8701 aatgccgata cccgaagcga tataggcgtt gaaagtcaaa cccaaatcgc gcgactgctt
     8761 gccgatggaa aacgcccgca ccaccagcca gccgtaacag aatatcagca cgcacatccc
     8821 gaagaagccg aattcttcgg cgatgatggc aaaaataaaa tcggtatgcg cttccggcag
     8881 aaagccgcgt ttgctcaaac tcgcacccaa acccataccg aaccactctc cgcgcccgat
     8941 tgccatcaga gagtgggtaa gctggtagcc ggcaccctgc gggtctttcc acgggtccaa
     9001 aaatgccact acccgctgca cacggtaggg agcggcggta atcatcagca ccatcccgcc
     9061 caagacgctg cctaccagga cgaaaaaata tttccacggc aatcctgcca aaaacagcat
     9121 tccaacggca atgacggtaa tgacgacaaa cgaaccgaaa tccggctgta ccattatcag
     9181 caccaaaccg aacgccacca gcataatcgg caggatgatc gcccggaaac ggccgtacat
     9241 ttctaatgtt tcacgacgtg cctgcggatt ggtggcggac atgatcagat tggccgtccc
     9301 ccgccaaatc gactgccaac ccaaactttc catgctgcgc aacacttctt cacggcgcgt
     9361 gaacaggctt gccaaataaa ggatgaccgc cagcttgaac agctcggtcg gctggaaatt
     9421 caacggaccc aaaggtatcc aacgggtcgc gccattgatt tcgcgcccgg caatcaatac
     9481 gactaccagc aacaggccgg atagggcaaa aatccacggc acaagccgcc gccatgtcct
     9541 catcctgcaa agaaaccata aaaaaccgct cgctatcaag ccggcaacga cgaaccccgc
     9601 ctgtctggtc aaatagaaaa actgatcgcc gccttctttt gatgccaaat acacagaagc
     9661 cgaataaatc atcagcaggc tgaacgccgt catcagcacc accatccaca aaagcggcgc
     9721 gtcgaatttc ctgccgtcgc gcacaatcgg cctgtcgagc agcagagtgt ggacaccgtc
     9781 gcccactttt accaatactt ccgaaatctt caaaaaaacc acctgccagt ctgtttgcac
     9841 ctgccgcaaa gggcaaaaat ttcagacggc agacaatgcc gtccgaacat acgatacatc
     9901 ccaaatcggt attctaaatc tttacttgcc gcccaacaat gacggcgttt gcgtttcaga
     9961 cggtatcaca aagccttaaa cgccccgata aacacttccg aacggtgcgc atagccttta
    10021 aacatatcga agcttgcgca ggcggggctg agcaacacaa tatcgcccgc ttcggcttgg
    10081 gcgtatgccg tctgaacggc ttcttccaaa gtggcgcagt cggtcatatt cagaccgcag
    10141 ccgtccaaat cgcggtggat ttgcggcgca tcgacaccaa tcaagaacac gccttttacc
    10201 ttgccggcaa gcgtatcgcg caggggcgtg aagtcctgcc ctttgcccat gccgcccaaa
    10261 atcacgaaga gaggattttg caaaccggca atcgcggcgg cggtcgcgcc gacgttcgtg
    10321 cctttgctgt cgtcgatgaa caccacgccg tttttctcgc caattttttc cacgcggtgc
    10381 ggcaggcctt ggaaggtttt gacgtgttcg agcaactcgt ttcgtggcaa gccaatagcc
    10441 tcacacaaag ccacggcagc cataacattg gcggcattgt gcaagccttg cagcggaatg
    10501 tcttgcgtgg caatcaaatc ttcatcgcct tgtttcagac ggcctgtctc gcgctccaac
    10561 caaaaatcgg cttggtgttc caacgaaaac cattttacct cgcgcccggc gcgcttcatc
    10621 gcgcgacaga acgcatcgtc cgcattcaaa acctgcacgc catcgccacg gaaaatcttg
    10681 tccttggtat gcgcatagtc gagcaagtcg tcgtagcggt cgagatggtc ttcggaaatg
    10741 ttcagcaccg tcgccgcagt cgggcgcagg ctttcggtgt tttccagttg gaagctggaa
    10801 agctccaaaa cccacacgtc cgccttttta ccttcgcgct gcaattccgc ctccaaaacc
    10861 ggcgtaccga tattgcccgc gatgacggta tccagcccgc acttgatgca gagatagccg
    10921 actaggctcg ttaccgtcgt tttgccgttg ctgccggtaa tcgcaatcac cttgtcgccg
    10981 cggcggttca caatgtccgc cagcaattcg atatcgccca acacacgtcc gccgttttgc
    11041 ttgaacgctt cgatatccgg ctgccgctcg ctgatgccgg gactgagcgc cagaatatca
    11101 aaaccgttgt ccagcgcatc tttcagacgg cctgtgtaaa acaccagccc gtcaaacatc
    11161 ttaccgattt gcgccacgcg ctcgggcttc aactccgcat cataagcggc aacctccacg
    11221 ccgtttttgc gcaggtaggc aatcatggaa atacccgtgc cgccaagtcc ggcgacgagg
    11281 atttttttgt tttggaaagt cattttggtt tgtcctaaaa caaatcatat tgggcaggaa
    11341 gatgtctgct cctgctcaag ccgctttcag acggcatcgc gagctgttca ataacccgct
    11401 ttcaggcgtt ggtaatggcc gcagccgtct tggtctctgt tttgacaagc cttaccgtac
    11461 cattcttggg caagggcgcg gtcttggcgc acgccgcgtc ctttgtaata cattgcgccc
    11521 aaattgtatt gggctgctgc atttccctgt gctgccgcct gcaagttttc ccgaaaatcc
    11581 gatacgtcat ccgcccacac cgcttggttc aagcccaagg caatcagggc ggcggcaagc
    11641 catttgactg tctgtttcat ggttttactt ctgttttagt ataagccgcc gttaacgata
    11701 gggctgggcg gattgtcgcc gcaggtttat tgcgcgttca aatgccgtct gaaaggtgtt
    11761 cagacggcat aggttcagcg gattttgagg gtactcaaac cgatcaacac caagacgatg
    11821 gtaataatcc aaaagcggac gacgacttgg gtttctttcc agcctttttg ttcgtagtgg
    11881 tgatggatgg gcgccatcag gaagatgcgt tttttggttt tcttatacca gccgacctga
    11941 agcataacgg atacggcttc tacgacaaat aatccgccca taatgacgag gacaaactct
    12001 tggcggacga tgacggcgac ggtaccgagc gcggcaccca atgccaatgc accgacatcg
    12061 cccataaaga cttgcgcggg ataggcgtta aaccacaaga aaccgaggca cgcgccgcac
    12121 atggcggtac agaaaatcac cacttcgttt gcgccggcaa cgtaaggtaa ttgcaggtat
    12181 tgggcaaatt gtgagtggcc gctggcatag gcgaagatgg cgaggccggc ggcaacgagg
    12241 acgacgggga aggtcgcaag gccgtccaag ccgtcggtga ggttgacggc attggatgtg
    12301 ccgacgatgg tcaggtaaga caacaccaaa aagccgacca cgcccagcgg cagggcgatt
    12361 tgtttgaaga acgggacaat caaaatattg ttggcggaat tggcggcaag gtaaaacaat
    12421 gccaaaccgg cgataatggc aacgcttgac tgccacacca ttttgaattt ggcggacacg
    12481 ccgttggggt ctttatagac gactttgcgc cagtcgtcgt aaaaaccgag tgcgcccgtg
    12541 gcgagcaata cgcccaagag aatccagata tacgggtttg cccagttgcc ccacaacagg
    12601 gtggacacgg taatggcggt cagaatcagc gaaccgccca tcgtcggcgt gccgtttttg
    12661 acgaggtggg tttgcggacc gtcggtacgc actgcctgcc cgcatttgag cgcggtcagc
    12721 ctgcgtatcg tccacgggcc gaacatcagg gaaaacgcta aggcggtcaa cgccgccatg
    12781 acggcgcgga atgtggtgta ttgaaaaata ttcagaccgg ttaaccagtt gctgaaatgt
    12841 gcgagccata aaaacatggg gcttcctttt tttgttttgt cgttatagtg gattaacaaa
    12901 aaccagtacg gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc
    12961 accaagtgaa tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    13021 ttttgttaat ccactataca tcgggcctgc gcccgtttga ggttggcttg ccgcccggca
    13081 aggtttcgga cggcttttgc agattaacgt gcagggactt tctcaatcac gcaaattggg
    13141 taactccccg gatttttacc gccgcccaag tctaaacata aatcttcata aagatacgcc
    13201 tgcgtccgca ttccggcata aaacgcgccg accagcgcgg caatcagcaa taaggcttta
    13261 aaaaaacgtc ggcttttcat tttcacttat cctccaatgc ctcgaccact tcttccatct
    13321 gcataaagcg cgaacctttc accaacacgg tggcgcgttc gggcaaatcg tggcgcaaca
    13381 cttgaatcaa cgggtctttg gcggcgaacc acaaaccgtc cgcgccaaat ttttccgccg
    13441 cttcgacgct gttgtcgccg acaaaataag ccgcttcgat gccttggtcg cgggcatacg
    13501 cgccgacttc ggcgtgcata gcggcggctt cgtcctcgcc cagttcgccc atatcgccca
    13561 ttacgaaaat acgcggcgca ggcatacgcg ccaacacgtc aatcgcggct ttcatgctgt
    13621 ccgggttcgc attataagta tcgtcaatca gggttgcgcc cttgattccg gatttgacgt
    13681 tcagacggcc tttgatattg ctgaagcctt tcaaaccttc cgccacatcg ttcaaactca
    13741 aacctgcagc caaagacagc gcggcggcag cggcggcgtt gtggacattg tggcggccgg
    13801 gaacaggcag caccacggcg gcgcgttcgt cgccgcacac caaatcaaat tcgcacgaca
    13861 acggtttcag cacgatattt tccgcgtgga catcgccgct atcgatgccg aaagtgcgcg
    13921 tattcaaatt aagcgttgcc gttttgaaga cagccatatt ggcatcttct tgaggaatca
    13981 gtgccatgcc gtctgaacat aagccttgat aaatctcgct tttcgctttg gcaatatcgc
    14041 ccactccgtc gaaaccgcag ccgacatggg cgcgcatggc gttgttgacc aatgcggcat
    14101 cgggtttggc gatttgtgtc aaaaccgcca gttcgccaaa atggttcata cccatttcaa
    14161 tcacggcata gcggtgtttt tcgtttaatt tcaacaaagt caacggcaat ccgatgtggt
    14221 tgttgaagtt gcctgccgtc gccaaaacgg cattatcgcc gaaacggcgg cgcaataccg
    14281 cagccaacat ttccttcacc gtcgtcttgc cgaccgaacc ggtaatgccg aacacaaacg
    14341 gatccacgtt ttcacgccac gcctttgcca gcgtttgcaa tgcggcaagc gtgtcatcaa
    14401 ctttcaacgc gtctttcaaa gacgcgcaat cttcgcgcga aaccacaacc gccgccgcac
    14461 ccgcagccaa tacgtcttca acaaaatcat gcgcgtcaaa ccgcccgccc gccaatgcga
    14521 aaaacacatc gcccgcgcgg atgtcgcggc tgtcggttac gatgcgcgac acgagtttgc
    14581 tttcagacgg catcggaagc ttgagggctt ggcagatgaa atttagatcc agtggtttca
    14641 tatttacttt cgttaatatt cgggcggcgg acacatcggt agcggctgat ttttttatcg
    14701 cctgttttgc tgtggtaaaa cacagattat tttcccattc tcattcggca ttttttctgt
    14761 acgtatcatt ttttagacgt atttttagtc gatttgcctt ttcccgcata ccacggcgcg
    14821 gggtcgtcgg gcagtccgtc gataaaggca aggttattgc cttcgccctg cacatcggga
    14881 acattccccc aaaaatcata gccgtcatcg ggcaactcgt cggtttcgat acccgtccaa
    14941 ctgccgaatc cgcgtaaaaa attaaacgcg ctgcgcctca ctttaatcat ttcgcgccgc
    15001 gtgtcatcgg acgaaatttt aaccagcggc acgccgtagc tttgacgctt ccacgcacca
    15061 tgtgtcaacg ttcgctcgcc gcctttaccg acatgcatca aaccgtggtc ggaaaagtaa
    15121 accaaagacc agctttcttt attttcattc aatatcttaa ctgtatcttc taaaaattta
    15181 tcggtttgcg cgatggtgga aacatagcag gatatttttt cagtttgata ctgaaaccgc
    15241 cgcgcatcct tatccaaacg tgtgcaaaaa tcactgtgcg aacccatcag gtgcatcaca
    15301 atcagccgag gcttcgtgcc tgcatttcct atcaaaaccc gtttgaacgc cggcaacaaa
    15361 aggcggtcgc tcaaccccgc gcttttgcca taatcacccc tttgggtaaa ccacggataa
    15421 tcgctgcgta gggcataggt ggaaatttcg ttggcaaaat gccccaacat tccttgatta
    15481 gacagccacg ccgtccgaaa acccgcctgc ttcgccaagc tgacgatgtt attgttcggt
    15541 tctcccggca gccccaaagt ctgcggcagc gaaagattcg tcgcgtgggc ggtcgattgg
    15601 taaccgttta tcaacagccc tttggtctga ctcaaaaaag gcgtatcggg caatgggaaa
    15661 ccgtaaacat tcatataatc cgaacgcgcg ctctcaccga taaccacaac ataattttta
    15721 tacttgggcg caacatgacg gatatgccat gttgacgctt tttttgctgc ttccaaaatg
    15781 tgggcgcgct tggcgacata ttgtgccggc gcggaagcca aatcgtaata caggtcgaaa
    15841 atattcaaca acaggccggc atcaggttct cgcaaatctt tatcgctggc gattttatcc
    15901 atcaccgcgc aggacaaaat caaagtcaat aatatagtca gccatatttt gctgcggcgt
    15961 ttatagtttt ttacgtcagc aaataccccc cccgatacac aatatttcca aactgtcaag
    16021 ccaaaaatga aaaataatgc ctgcacaaaa taaagcgacc cgggaagatt gccgacaaat
    16081 tcacgcgcct cggcaggatt gctttccaat atcgaaccga ctatctgata agacggcgca
    16141 ccatacaacc agccgaccgg caaatatagg gcggttgtgc cgacgtaaat cagtaaaaca
    16201 actgaagaaa cgcgcgggaa accgcgtgcc aataataata aaaatacaac cgaagccaaa
    16261 gcacccacgg cagaataacg gtagccgtat tcatattcca agtgataacc tgatgcgatg
    16321 gccgcgccaa aacaaaaggc taaaagcgcg caaatgccca atgtattttt taatgtctga
    16381 tccatatttt ttattcgggc ggattgtttc atacgatgcc gtccgaatat cccattattt
    16441 acgagttaac aaagcctgtc cgacgatttc aagatcggaa aaacggtgct tcacgccttg
    16501 tacatcctga tagttttcat gccctttgcc ggcaatcagg atgatgtcgt ttgcggcggc
    16561 ttgttcaacc gcataacgga tggcggcggc acggtcggct tcgacacgtt cgggattagg
    16621 tacggcaggc aggatgtcgt tgatgatgtc gtgcggattt tccaaacgcg gattgtcgct
    16681 ggtgacgacg actttatccg cgccctgtac ggctgccgcg cccatcagcg ggcgtttgcc
    16741 gagatcgcgg ttgccgccgc aaccgaatac gcaccacaaa gccgcaccct gcggtttgat
    16801 ttcctgcaag gtggcgagtg ctttttccaa tgcgtcgggc gtgtgggcgt aatcgacgac
    16861 gaccaagggc ttgccgctgt tcatgatgca gtccatgcgc cctgaagcgg gacggatttt
    16921 tgccagcaca tccaatactt tatcaagcgg atagccgttg gcgcacagca aggcgatgca
    16981 ggcggcgagg ttttgcgcgt tgaaccgtcc gagcaggcgc gtgcggcatt tcccttcgcc
    17041 ccacggggtt tggaatacgg ctgctatgcc gtctgaagag gcggtaaagt cggtaatgcg
    17101 gatgtcggcg tgttcgctga aaccatagct gtaaacggcc aaatcgggac agtctttttt
    17161 cagacgacct acgagttccg cgccgtattc gtcatccacg ttgatgactg cgtgtttcaa
    17221 gccgtgccag taaaacaggc gcgacttgat ggcaccgtag gcttccatcg tgccgtggta
    17281 gtcgaggtgg tcgcgggtga ggttggtaaa gattgcgctg cggaatgaca cgccgttgac
    17341 gcgcgactgg tcaagcccgt ggctggagac ttccatcgcg gcgactgttg cgccttgttg
    17401 acggaaacgg tagagcaggg tttggacatc gacgggggcg ggtgtggtat gcgtggtttc
    17461 ttccaatgca ccccaaaagc cgttgccgac cgtgccgaca atggcggttt tttcgcccaa
    17521 caaatcggca gcttgcgcca gccattgcgt gatggaggtt ttgccattgg tgccggttac
    17581 gccccagact ttgagaccgt ctgaaacgtt gccgtaaact tgcgccgcca atatgccggc
    17641 acggtgtttc aaatctttga tgccttgatt ggggactttc cattcgggat tccacgcgaa
    17701 tttgccatcg tcgtcccaaa aaacaaaagc cgcgccgttg gcaacggcgg cggggatata
    17761 actgcgtccg tcggcatatt cgcccggaca ggcaacgaaa atatcgcctt gtttgatttg
    17821 gcggctgtct gaatgcaaca aacgccctgc cgcgtttgca cacgacaggg tcgggatgcc
    17881 ggtttcagcc aaaggggtta acttgctgaa cataaaacaa tctcgttgat actcggatta
    17941 agacggtgtt ttgacggctg cggcggtcag gggcttggtc ggggaaatgc ccaagatgtt
    18001 caggctgccg cccataattt ttttgaaggg cggccctgcc actacgccgc cgtaataacc
    18061 gtgggcagtc ggttcgtcaa tggttaccgc cacaatcaca cgggggtttt tggcgggggc
    18121 aaaaccgata aaggtagcga tgtgtttgtt gtcggcataa cgcccgttga cgaacttgcg
    18181 cgccgtgccg gttttcgcgc cgacatcgaa accgtccacc gcacccgccg taccggtgcc
    18241 gcccggctcg gttacggaaa ccatcagatt gcgtacctcg cgcgcggtcg attctttgaa
    18301 tatgcgtttg ccttgcggcg caaccgcctg tttttcaaag ctgaccggca gtaaaacgcc
    18361 gtcgtgcgtc agtgcggtat aggcgcgcgc caattgcagc aggctcaatt gcaggccgta
    18421 accgaaagac atcgtcgcct gttcgatagg ccgccacctg cgccaatttc tcaacaaacc
    18481 tgcagtttcg cccggaaagc ccgagtgcat acgcacaccg atgcccagct catgatagaa
    18541 gtcatacatt tcttcggcac cgaaacgcgc agacagtttg cttgtgccga cgttggacga
    18601 tttctgcatg atgccgcgca catccaaaga ggggtaaaca tgggtatcgc gcacgggaga
    18661 cggtccgatt ttataaggct gcgtattcag ccgttcgttc aaatcggttt tgcccgcatc
    18721 caatgccttc gcaatcacaa acggtttgat tgccgaaccg ggttcgatca tatcggttac
    18781 ggcacggttg cgccgctgtt cgctgtctgc ccggccgggc ctgttgggat cgtaggcggg
    18841 cgtattggcc aaggcgagga tttcccccgt gcgggcatcc aaaaccacca ccgttccggc
    18901 ttttgcctga tggtattcga ccgccttgtt caactcttca taggccaagg tctgaatcct
    18961 ctgatcgagg gaaaggatga tgtctttgcc gtttttcggg gctttattgc gcggggagtc
    19021 caagctgtcc acaatattgc cctgccggtc ccgcaaaacg acttccgcgc cgtcttcgcc
    19081 atgcaggctg tcttcaagcg aaagttccaa accttcctga cctttgccgt caatatcggt
    19141 aaatccgatg acgtgtgcaa acaggttgcc catcgggtaa tggcgtttta attctttttc
    19201 aaatacaaag ttttccaaac ccaaggcttt gacctcttcg gcaaccttgg gatcgagctg
    19261 ccgcttaatc cagataaacg acttgccttt ctgttcgagc ttgttcctca aaacatcaac
    19321 cggcacatcg acaagctcgg acaggcgttc caattgtgcg gcagacggca tttccttcat
    19381 ctctttaggc acggcaaaca gggactccgt cggcgcactc aacgccaaaa ccgcaccgtt
    19441 ccggtccgaa accgtaccgc gtgtagccgg caatgtttga gtccgcacaa tccggttgtc
    19501 gccctgttct ttcaaaaagt tatacgttac cgtctgcaga tacagtccgc gagcaatcag
    19561 accggcaaac aagaccgcta ttgccatcag gacgaagctg atccgtccgt tactggtcat
    19621 cggctttttg acctgctctt ctttgggcag catccgaggc ttatattcgc tcttaatcaa
    19681 catttttact tctcgttatt attatcctga cgcaggaatc cgattccggc acacaggctg
    19741 cttctatctt tgatgcgcca ccataaaggt attgcccgaa accggcggat ggaggttttg
    19801 tttttctgcc gccgccctga tcgcttcgtg gttcgccaaa cgcgcctgtt gcagcctcat
    19861 ttgcgcataa tcctgctcca aggcgatttc ctgttttttc gccttgtcca aagctgtgaa
    19921 gttgagcctg tactggtttt gctgcatcac aacggaaaaa gcggaaacgc acaccgcaag
    19981 cagcagaagg aaattcaatt tgttcattgc cattcagacg tttttctctg tgattgttcc
    20041 ggtatcgaac cggcagtccg ctccgccaca cgcaaaaccg cacttctcgc cctcggattg
    20101 gcggcaattt ccgcctcacc cggctttaat gccctgccca cgattttcag gggcagctcg
    20161 ggcaaatccg cttccctgac cgccgcccaa cgcggcaggg gcgcgtgttg cgaatatttt
    20221 ttgacaaact gcttcacaat gcggtcttcc aacgaatgga aagcaatgac cgccaaacgt
    20281 ccgccctctt tcagacgaca catgacctgc ggcaatactg cccctacttc ttcaagctcg
    20341 cggttaataa agatgcggac cgcctggaag gtgcgcgtcg caggatcctg cccccgctcg
    20401 cgagtacgga cgttttgtgc cacgatctgc gccagcttgc gggttgtatc gattggactt
    20461 tccgcccgtt gcgcaacaat ggcgcgcgca atctggcggc taaaccgctc ttcaccataa
    20521 ttcttgatta cctcgtgcaa atcctgttcc gacgcaaccg ctatccactc tgcggcagac
    20581 ataccgcgcg tcgtatccat acgcatatcc aaaggggcat cgaaacggaa gctgaaaccg
    20641 cggctgccgt catcgatttg cggagacgaa atccccaaat caaacagcgc accgtccacc
    20701 ttgccgatac ccaaaccgtc caatgccgtc tgaaacgaag caaaaccgcc atgcacgaca
    20761 ccgacccgtt tgtccgaacg cgccagctct tctgccacag caatcgcctg cgggtctttg
    20821 tcgaaaacaa tcaaccgccc cgcatcgccc aaacgcgaca aaatcagccg ggaatgccct
    20881 cccctgccga acgtaccgtc cacatagaca ccgtcttcgc gcacggcaag cgcatccacc
    20941 gcctcattca gcaagaccgt gatatgccgg taactttctg ctccactcac aattgcaaat
    21001 ccgtctgact caactggaag gcaagttcgt caggatcgtc atccaaagcc tgaaccatct
    21061 cagcctccca ctgctcgcga ccccaaagct ccaaacggtt ggcacgaccg accaaaacga
    21121 cttcacggtc gaaatccacc ctcttcctca gtccggcagg aaccagcacc cggccggcgc
    21181 tgtcccattc caaaatttcc gcgttatgca gcaaaagatt ttgaaaccgc cgcaaaacag
    21241 ggttatccgc cacttttaag tttaaaagtt gcgccgcaac cttttcccac tccgcaacag
    21301 ggtacatcaa cagcttgtgt ttcgactcga gcgttaccac tacggcaggc gtatagaggc
    21361 gcgacagaat gtcacggaat ttggcaggaa cagccaaccg ccccttactg tcgatgctta
    21421 attcgtgtgc gccgccgaac atgacatgtc ccaagccgaa atcaaaatca caagggtaaa
    21481 agagacactt tgccccacaa ttccccacca atcgacacta taagaaattt taaacactcg
    21541 gtcaaatcag ggcatgaaaa ctcattaaca tatctgaaat ttttattcct tttaaaaaca
    21601 ataagataaa aaatgacgac aacggcacgg cgggtgcggt acagaataat cgaaccaata
    21661 aacaactata tatgattaat ttaataatat aaacacaata tatagtatta agataaagcc
    21721 atgacagcac ccgtaccaac gtgtaatatg tcgggaaatc caataaattt acacaagcta
    21781 acacttatca tgcccctccc cctcccctct cccgaagcac ggcaattctc gctcaaactg
    21841 caaaccctca ttgccgaaga aatcggcaaa cacggcaact ggattccatt ttcacgtttt
    21901 atggaattgg ttttatacgc tccgcaatac ggctactaca ccggcggcag ccataaaatc
    21961 ggcaataccg gggattttat taccgcaccg accctcacct ctctgtttgc acagacactg
    22021 gcacgccaac ttcaagaact tctatcccaa acggcgggca atatctatga attcggcgcg
    22081 ggaaccggac agcttgccgc cgatttgttg ggcagcattt cggacggcat cagccgttac
    22141 tatattattg aaatatcgcc ggagctggca gcacgtcaga aaaacctgat tcaagcacgc
    22201 gcaccggaag catctcaaaa agttgtccac ttgaccgcac ttcccgaagc gtttgacggc
    22261 atcatcatcg gcaacgaagt actcgatgcc atgcctgtcg aaatcgtccg taaaaatgaa
    22321 ggcggctcat tcgagcatgt cggcgtttgc ctagataatg accgttttac ctattcggca
    22381 cgaccgctgc acgacttaca gctatctgcc ttggcttccc tctattttcc tcaaacagat
    22441 tatccctaca ccagcgaact acatccgcaa caatatgcct ttatccgcac ccttgcctca
    22501 aggcttgaac gcgggggtat gatattcatc gactacggtt ttgatgcagc gcagtattac
    22561 caccctcaac gcaatcaagg tactctgatc ggacactacc gacatcacgt tatccacaat
    22621 ccttttgact tcatcgggtt ggccgacctg accgcgcatg tcaactttac cgacattgcc
    22681 caagccggta cggatgccgg attagatttg ataggttacc ttccccaatc ccatttctta
    22741 ttgaacctgg gcattaccga gctattggca cagacgggga aaacgaattc ggcagcctat
    22801 atccgtgaag ctgctgccgt tcagaaactg attgaccagc atgaaatggg cgaactgttt
    22861 aaagtcatcg cattcggaaa aaatatcggc atcgactggg caggattcag cttcggcgac
    22921 atctgccaca aactctaacc ctcatgccgc ctgaatccgc ttcagacgac ataaactttt
    22981 taacatttaa aaacagtcaa ctaattcaaa attaaaaaat acggcttgtc aaaaaaacag
    23041 aaaaacatat aatagcgtct tcacgaaacg gcgaattagc tcagtcggtt agagcagagg
    23101 aatcataatc cttgtgtccg gggttcgaat ccctgattcg ccaccaaatt ttcgggggta
    23161 tagctcagtt ggtagagcgc ttgcatggca tgcaagaggt cagcggttcg atcccgctta
    23221 cctccaccag ataaaaaagc acagaccgta aaaaggtatg tgctttttta ttgcctgatt
    23281 gccagcaaat aaagaataaa ccactgcctt caaaacaggc aatcgacttt aaacctatcg
    23341 ccccgcctgt cctgatttta tagtgaatta aatttaaacc ggtacagcgt tggctcgcct
    23401 tgccgtacta tctgcggctt cgtcgccttg tcctgatttt tgttaattca ctatatcagc
    23461 ccgccagaca aacccgaccc gaataatgtc ttcaggtcgg gtttatggtt tcattcccaa
    23521 cttatccagc ctgacagcca caatataatg atggcaatac caaaaacaat gcggtaatag
    23581 gcaaaaggaa tataattttt cttggaaaca aacctcagca acgctttgac cgccaccaag
    23641 cctgaaacaa aggcagcaac aaagccaatc aaaatcaaac cgacatcatg cagggtgaaa
    23701 aaccggtaat gcttcaggac atcataagcc gttgcggcaa ccatcatcgg cacagccaag
    23761 aaaaacgaga attctgtcgc agttttccgt tcgattcccc aaagcatccc gcccataatc
    23821 gtactgcccg aacgggacgt acccggaatc agtgcaaaca cttgggcaac gccgatcatc
    23881 aaggcatcaa tcggacgcaa tgcatcaaca tcggcaattt taggctctgc ccagctttgg
    23941 cgtttctcca cccacaaaat aaacaaaccg cccaaaacca gcatgaccgc cacactcaaa
    24001 ggattgaaca gatgttcttt aatctgtttg ccgaacaaca gccccatcac ggcggcaggt
    24061 ataaaagcaa tggcaagatt gaggacgaag cggttggctt tccggtcttt tcccacgcca
    24121 tgcaacacat tgctgaaacg ctgccggtat tcaaacacta ccgccaaaac tgcaccgagc
    24181 tggatggcaa tttcaaaaac cttgtgattg ctgtgaaaat caatcagatt gccgaacaca
    24241 atcaaatgcc cggtgctgga aatcggtaaa aattcggtaa aaccttctac caagcccatc
    24301 atcagggctt tcaggacaat cagaaaatcc attgcttgcg cttctttcgg atacgggagt
    24361 tcggctattt ctgtacagca ggggtctgac gcttgcgttc ttccctgact ttggcaacca
    24421 attctttaat cgtatgaaca ccgccgtcaa agccattatt gaagataacg cggtattttc
    24481 cgccgacgac aacggtcggc gtgctgtcaa taccgtattg ttccgtcagt ttctgcattt
    24541 ttgatgctgc tgctgcggcc tcgggagaat cataggcgcg catcagtttt ttgccgtcaa
    24601 actgcttttg ggacaaagcc cattttccgg caacagccct gttttccaag cggatttttt
    24661 gttcgtaaac tgctttaaac acagcagggt ttgcctgata tttcaaaccc gacaaattga
    24721 cggcagccgc catcctggcc agaccgagca tttcaggctg ccagaccacg tgctccgtcc
    24781 tcaaataggc atcagacggc aatgccttac ccagtttcaa taacaaagga tcgaaatgat
    24841 ggcaatgtac gcagaaatag ccgaaaaatt ccaaaacctc aattttaccc gactgttctt
    24901 gaggaatggg tttatccaac acaagatagt cttccccttc cgtcagggca tatgcctgcg
    24961 cggacaacac tgccgacagc agcagcggca acagatgttt gagcttcata attatttact
    25021 ttcgatagaa cggatcaggc tggcgacttc atgttttttc aactcgtcct gcattttttt
    25081 caccgcatcg gcagacatat tgccgctttg cacccggtaa agcgttttat gtcccgcctg
    25141 ataaccgacc accttggaag atatgcccaa gattgccagt ttggcacgct gcccttccgc
    25201 gctccggcgg tcggcatacg cgcccatttg cagataatgc gttgcttccg ccttgtcgga
    25261 cgttttcatt ttctgcactt ctttggcggc ggcactgcgc gctttttcga tgctgccgct
    25321 gttgaggatt tgttccgggg tcggtttggg tgcaactttt tccttcgccg cctttttctc
    25381 ttcttttgga gcttttttct ctgtttcttt agacggtttt accgcttgtt ttttaaccgt
    25441 ttcggcatct ttcttctgcg ctttttccct gacggtttgt tcacgctctt ccgtcagtgc
    25501 tttcttacgc actgcctgtc cgtccggctc gcccgccttt tcttcaacct cgtcggcttt
    25561 atcggcagct ttttccgcat ccgactgctc tgcctctgtc gcagcatccg gttcgtacaa
    25621 ggcgttttga tcggccggtt caggttggat gtcttcctta ggctggtttt tcggtttcag
    25681 gatttccgtt tctgcaggct gcttcgacgg aaccgggatt ttgaacgcat tttgaccgct
    25741 ctggttcaga taaaacaaaa taccggcaat aatgaccgtt gccagtatca aaccgaagaa
    25801 aaaaccggac agaccttttc cggattggga aaatttgttc ataaacatac cttaatgtgt
    25861 ttcagacggc attaacgccg tttgcttacg ggcggatatt ctaacaatat cgccatattt
    25921 gggcaaaacc tgcttccatt cccgttccta taaagcgcgg cggaaaccta tggctttcat
    25981 gacgtggctt ctgccgactt cttcgtcgcc cgccaaatcc gccaatgtac gcgccacgcg
    26041 catgatgcgg tggaaactgc gggcggagag ggagagtttt tccagcaggc tgcccaatgc
    26101 ttcctgcgct tctttttgga tgcgggcttg ggaatcgagt tcgctgacgc tcaaagcggc
    26161 gttgactttg ccctgccgtg cgtattgttt gtcgcgggcg gcgataacgc gttccaaaac
    26221 ggacgcgctg ctttcgcccg cttcctgctg catcagttcg gcggcggaca ggctggggac
    26281 ttcgatggtc aaatcgatgc ggtcgagcag cgggccggaa atcttgctgc ggtaacgcgc
    26341 gacgctttcg ggcgtgcagc ggcagggttt gacgggatgc ccgagataac cgcacggaca
    26401 cgggttcatg gcggcgacaa gttggaattt ggcaggatag acggcttggc gcgccgcgcg
    26461 ggaaatgtgg atttcgccgt tttccaacgg ttcgcgcaaa acttccaaaa ctttgcggtc
    26521 gaactcgggc aattcgtcca aaaacaaaac accgtgatgc gccaatgaaa tctcgcccgg
    26581 acgcggatcc gaaccgccgc cgaccatagc cgccgcgctg gcgctgtgat gcggactgcg
    26641 gaaaggacgg ttgctgtcga gttgttgctg gtggttgggc aggagcgaac gcaatgccca
    26701 aacttcaacc aattcgtctt cggtcagcgg cggcaggatg ccgggcagcc gttgggagag
    26761 catagacttg cccgttcccg gcggacccat catcaagagg ctgtgtccgc ctgcggcagc
    26821 gatttccaaa gcaaggcggg cagtgtgctg acccttgaca tcgacgaggt cgagcaattt
    26881 ggtgttttca gacggcatct gaggaacttg acagtcggtt tgcaccaaag gttcgatgcc
    26941 gttcaaatgg gcggcgactt cgcccaaaga gcgcgcgccg taaacggtaa tgccgcgcat
    27001 cacggcggct tgtcctgcgt tttcttcagg caaaacaaat gcacgttttg cctgcatacc
    27061 ctgccacgcc atcgccaacg cgccgcgcac ggggcgcaac agccccgaca gtgccaattc
    27121 ccccgcaaac tcgtattccg ccagtttttc gggcgcaacc tgccccgatg cggcaaggat
    27181 gccgattgca atcggcaaat cgaaacgccc cgactctttg ggcaggtcgg cgggggcgag
    27241 gttgacggta atttttttgg cggggaattc aaaaccgctt tgaataatgg cggcacggac
    27301 gcggtcgcga ctttccttta cttccgtatc gggcagcccg acgatgttga aatgcggcag
    27361 gccgttggca aggtgggctt ccacttcgac caacggcgca ttcataccgc tcaaggcgcg
    27421 gctgtaaacc aaggcaagcg acatatttca gacggcctta ttcgccggct tcggtttgct
    27481 gcctgatttc ggcgacggct tcttcggcag cggcttcagc cgcttccaat gctgcccgtt
    27541 cgggattttg cgcggcttcg agttttgcca aacgcgcttc caaagccgcc agtttggtac
    27601 gagttttgat taaaacctgc tgctggatgt cgaattcttc gcgcgtaacc agatccatac
    27661 ggttgaacgc gccgcccagc atcgccttaa tatttttttc cacatctttg gcagggctgt
    27721 tggcgatggt ttcgctgatt ttcgaaccga cttcttcaaa aagctgcttg ccgaacataa
    27781 tctgtatcct tcctgaacat atcaaattca atcggctatt gtataaggaa aaatgccgtc
    27841 tgaaaacggg cggcggataa tcggcaaaac ataccgcgcc tcctttgcgg ttgctaaatt
    27901 acacctcaaa acacctgccg cctgaaaacg gatttcatat tccgcgcaac gccagattac
    27961 aagacactac aaaacaatat actgttttaa atgatttttc cgacgcgcat cgtttcaaac
    28021 tcggcttttt aagccattaa gtgctttgca aacaacagaa attgggttat cctgaaacgg
    28081 attatttaca atttcatata gttttattac atatcttatt gtgattgaag ataatttatc
    28141 cgaatccccc tttcgggtat ccggattttc cgttgtactt ttattagaaa aacatttcag
    28201 gcgcaagttg cttgcaattt caaagcctgt aattctgaag ttttagacga ggaatagcac
    28261 atgaatccca tgtacattac ttttgcaatc tatctggttg cagtccttct catcgggctt
    28321 gccgcctatt tttccacgcg caatttcgac gattatattt tgggcgggcg cagcctaggc
    28381 ccgtttgtta ccgcgatgtc ggcaggcgcg tccgatatgt ccggctggct gctgatgggt
    28441 ctgccgggcg cgatttacct gagcggtttg aatgaggctt ggattgccat cggcctcttg
    28501 gtcggcgcgt atttcaactg gctcttggtg gcgggccgcc tgcgcgtgca taccgaatac
    28561 gccaacaatg cgctgacgct gccggattat ttcttccacc gcttcggcgc gggcggacac
    28621 ttgatgaaag tggtttccgc actgattatc ctgttcttct tcacgattta ttgcgcctcg
    28681 ggcattgtgg cgggcgcaac cctgttccaa agcctgtttg aaggtatgac ttacaatcag
    28741 gcaatgtggc tgggcgcggg cgcgaccatc gcctatacct tcttgggcgg tttcttggcg
    28801 gtcagctgga cggatacgct gcaggcttct ttgatgattt tcgccctgat tttaacgccg
    28861 gtgatggtct atctgggctt gggcggcgcg gaacagatgt ctgccgcgat tcaaagcgtt
    28921 gccgcaggca cgggtaaaga atacggcagc ctgtttacag gtacgacggt tatcggcatt
    28981 atttccactg ctgcatgggg attgggctac ttcggccaac cgcacatcct ggcgcgcttt
    29041 atggcggccg aaagcgcgaa atcgctggta tccgcacgcc gcatcggcat gacttggatg
    29101 gcgttgtgcc ttgcgggcgc ggtggcggtc ggttatttcg gcattgcgta tttcggtgca
    29161 aatcccgaca aagtttcttc tatgagcggc aaccacgaac gcatcttcat cgcgctttcc
    29221 accctgctgt tcaacccttg gatcgccggc atcattttga gcgcgatttt ggcggcggtg
    29281 atgtccaccc tgtcctgcca gcttttggtt tgctccagcg cgattaccga agacttctac
    29341 aaaggctttt tgcgtaaaaa cgcgcaacag tcggaactgg tatgggtcgg ccgcctgatg
    29401 gtcttggcga ttgccgtgat ttccatcctg attgcttccg atccgaacag caaagtattg
    29461 ggactggtgt cttatgcgtg ggcaggtttc ggtgcggcgt tcggcccgat tgtgattctg
    29521 tccgtattgt ggaaacgcat cactgcctac ggcgcgttgt cgggtatggt tgcaggtgcc
    29581 ttaaccgtag tcgtatgggc ggaatggata aaaaaaccgg ctcaagcagc aggcgaaagc
    29641 ggcttgttga cgatgtacga aatcgtcccg ggctttattg tttgcttaat cattgccgtg
    29701 ttggtttccc tgttcaacaa agaaccttcg cgcgaaatcc aagaacgctt tgaaaaagcc
    29761 gacgcagatt accgcgccgc ccgataaacc cctttcagac ggcacggcat cccggttcgt
    29821 gccgtctgaa cccgtttccg ggtcaaacac aaataaacct tgcctgcata caggccttat
    29881 ttgtgtttta cccgtttcat tttcaaagaa acggaagcac ctaccctgcc cgcatttgaa
    29941 gccttgtatt tcatctgcgg atttattacg atattaccgt attcagaccg caccgatgcc
    30001 gtctgaaccc cccgaaaaac tttggagaat ccaaaaaatg tttcattttg catttccggc
    30061 acaaactgcc ctgcgccaag cgataaccga tgcctaccgc cgtaatgaaa tcgaagccgt
    30121 acaggatatg ctgcaacgtg cacagatgag cgacgaagag cgcaacgccg cctccgagct
    30181 tgcccgccgt ttggttaccc aagtccgcgc cggccgcacc aaagccggcg gcgtggatgc
    30241 gctgatgcac gagttttcac tctccagcga agaaggcatc gcgctgatgt gtctggcaga
    30301 agccctgctg cgtatccccg acaacgccac gcgcgaccgc ctgattgccg acaagatttc
    30361 agacggcaac tggaaaagcc atttgaacaa cagcccttcc ctcttcgtca acgccgccgc
    30421 ctggggcctg ctgattaccg gcaaactgac cgccacaaac gacagacaaa tgagttccgc
    30481 actcagccgc ctgatcagca aaggcggcgc accgctcatc cgccaaggcg taaattacgc
    30541 catgcgcctg ttgggcaaac agttcgtaac cggacagacc attgaagaag ccctgcaaaa
    30601 cggcaaagaa cgcgaaaaaa tgggctaccg cttctccttc gatatgttgg gcgaagccgc
    30661 ctacacccaa gccgatgccg accgctacta ccgcgactat gtcgaagcca tccacgccat
    30721 cggcaaagat gcggcaggac aaggcgttta cgaaggcaac ggcatttccg tcaaactttc
    30781 cgccatccat ccgcgctact cgcgcgccca acacggccgt gtgatgggcg aactgttgcc
    30841 gcgcctgaaa gagctgttcc ttttgggtaa aaaatacgat atcggtatca acatcgatgc
    30901 cgaagaagcc aaccgtctgg agctgtcttt ggatttgatg gaggctttgg tttcagaccc
    30961 tgacttggct ggctacaaag gtatcggttt cgttgtccaa gcctaccaaa aacgttgtcc
    31021 gttcgttatc gactacctga ttgacctagc ccgccgcaac aaccaaaaac tgatgatccg
    31081 cctcgtcaaa ggcgcgtatt gggacagcga aatcaaatgg gcgcaagtgg acggcttgaa
    31141 cggctatccg acctacaccc gcaaagtcca caccgacatc tcctacctcg cctgcgcgcg
    31201 caaactgctt tccgcgcaag acgcggtatt cccgcaattt gccacgcaca acgcctacac
    31261 cttaggcgca atctaccaaa tgggcaaagg caaagacttt gaacaccaat gcctgcacgg
    31321 catgggcgaa accctgtacg accaagtcgt cggcccgcaa aacttaggcc gccgcgtgcg
    31381 cgtgtacgcc ccggtcggca cacacgaaac cctgctcgcc tacttggtgc gccgcctgtt
    31441 ggaaaacggc gcgaactcgt ctttcgtcaa ccaaatcgtc gatgaaaaca tcagcatcga
    31501 cacgctcatc cgcagcccgt tcgacaccat cgccgaacaa ggcatccacc tgcacaacgc
    31561 cctgccgctg ccgcgcgatt tgtacggcaa atgccgtctg aactcgcaag gcgtggactt
    31621 gagcaacgaa aacgtattgc agcagcttca agaacagatg aacaaagccg ccgcgcaaga
    31681 cttccacgcc gcatccatcg tcaacggcaa agcccgcgat gtcggcgaag cgcaaccgat
    31741 taaaaaccct gccgaccacg acgacatcgt cggcacagtc agctttgccg atgccgcgct
    31801 tgcccaagaa gcggttggcg cagccgttgc cgcgttcccc gaatggagtg cgacacctgc
    31861 cgccgaacgc gccgcctgcc tgcgccgttt cgccgacctg ctcgaacagc acacccccgc
    31921 gctgatgatg cttgccgtgc gcgaagcagg caaaacgctg aacaacgcca ttgccgaagt
    31981 gcgcgaagcc gtcgatttct gccgctacta cgcaaacgaa gccgaacata ccctgcctca
    32041 agacgcaaaa gccgtcggcg cgattgtcgc catcagcccg tggaacttcc cgctcgccat
    32101 ctttaccggc gaagtcgttt ccgcattggc ggcaggcaac accgtcatcg ccaaacccgc
    32161 cgaacaaacc agcctgattg ccggttatgc cgtttccctc atgcacgaag ccggcatccc
    32221 gacttccgcc ctgcaactcg tcctcggcgc aggcgacgtg ggtgcggcat tgaccaacga
    32281 tgcccgcatc ggcggcgtga ttttcaccgg ctcgaccgaa gtggcgcgcc tgatcaacaa
    32341 agcccttgcc aaacgcggcg acaatcccgt cctgattgcc gaaaccggcg gacaaaacgc
    32401 catgattgtc gattccaccg cacttgccga acaagtctgc gccgacgtat tgaactccgc
    32461 cttcgacagc gcgggacaac gctgctccgc cctgcgtatc ttgtgcgtcc aagaagacgt
    32521 tgccgaccgt atgctcgaca tgatcaaagg cgcgatggac gaactcgtcg tcggcaaacc
    32581 gattcagctc actaccgatg tcggccccgt catcgatgcc gaagcacagc aaaacctgtt
    32641 gaaccacatc aacaaaatga aaggcgttgc caagtcttac cacgaagtca aaaccgctgc
    32701 cgatgtcgat tccgaaaaat ccacgttcgt ccgccccatc ctgtttgaat tgaacaacct
    32761 caacgaactg caacgcgaag tcttcggtcc cgtcctgcac gtcgtccgct accgcgccga
    32821 caaactcgac agcgtcatcg accaaatcaa cagcaaaggc tacgccctga cccacggcgt
    32881 acacagccgc atcgaaggca cagtacgcca catccgcagc cgcatcgaag ccggcaacgt
    32941 ttacgtcaac cgcaacatcg tcggcgcagt cgtcggcgta cagcccttcg gcggacacgg
    33001 tctgtccggc acaggcccca aagcaggcgg ttcgttctac ctgcaaaaac tgacccgcat
    33061 ccccgaatgg gttgccccga ccctgagcca aatcggacag gcagacgaag ccgcgctcaa
    33121 acgcctcgaa gcactggttc acaaactgcc gttcaacgcc gaagagaaaa aagccgcagc
    33181 ggccgctttg ggacacgccc gcatccgcac cctgcgccgt gccgaaaccg tccttaccgg
    33241 cccgaccggc gagcgcaaca gcatctcatg gcacgcgcca aaacgcgtct gggtacacgg
    33301 cggcagcctc ctgcaagcct tctgcgccct aaccgaactt gccgcctccg gcgtacaggc
    33361 agtggtcgaa cccgacagcc cgctggcttc ctacaccgcc gacttggaag gtctgctgct
    33421 ggtcaacggc aaacccgaaa ccgccgacat cagccacgtt gccgccctgt cgcctttgga
    33481 cagcgcgcgc aaacaggaac ttgccgccca cgacggcgca ctcatccgca tcctcccttc
    33541 ggaaaacgga ctcgacatcc tgcaagtgtt tgaagaaatc tcttgcagcg tcaacaccac
    33601 agccgccggc ggcaacgcca gcctgatggc ggttgccgac tgattttacc gaaatccccg
    33661 agcgcggccc gtgaaccaat gccgtctgaa aacctttcag acggca
//
LOCUS       DAWFJW010000010        32135 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFJW010000010 DAWFJW010000000
VERSION     DAWFJW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.010"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(68..814)
                     /locus_tag="WGR19_000377"
     CDS             complement(68..814)
                     /locus_tag="WGR19_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0797972.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WGR19_000378"
     CDS             complement(993..1664)
                     /locus_tag="WGR19_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0797973.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGR19_000379"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WGR19_000379"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0797974.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKII
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WGR19_000380"
     CDS             complement(2481..2963)
                     /locus_tag="WGR19_000380"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0797975.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WGR19_000381"
     CDS             complement(2995..3684)
                     /locus_tag="WGR19_000381"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0797976.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPIADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGR19_000382"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WGR19_000382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0797977.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WGR19_000383"
     CDS             complement(4571..5881)
                     /locus_tag="WGR19_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0797978.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSEMVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLRLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WGR19_000384"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WGR19_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0797979.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WGR19_000385"
     CDS             complement(6056..6355)
                     /locus_tag="WGR19_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797980.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WGR19_000386"
     CDS             6706..7902
                     /locus_tag="WGR19_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0797981.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPNHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8116..8178)
                     /locus_tag="WGR19_000387"
                     /pseudo
     CDS             complement(8116..8178)
                     /locus_tag="WGR19_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8467..10308)
                     /locus_tag="WGR19_000388"
     CDS             complement(8467..10308)
                     /locus_tag="WGR19_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0797982.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PESAKDRFLQPSSQNGSGVLPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFVLGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANMP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10399..10869
                     /locus_tag="WGR19_000389"
     CDS             10399..10869
                     /locus_tag="WGR19_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0797983.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     CYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKHGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            complement(10942..11754)
                     /gene="hpnC"
                     /locus_tag="WGR19_000390"
     CDS             complement(10942..11754)
                     /gene="hpnC"
                     /locus_tag="WGR19_000390"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0797984.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLDALRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LNGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(11841..12323)
                     /locus_tag="WGR19_000391"
     CDS             complement(11841..12323)
                     /locus_tag="WGR19_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0797985.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12796..13077
                     /locus_tag="WGR19_000392"
     CDS             12796..13077
                     /locus_tag="WGR19_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797986.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAPATEAPAAEAPATEAPAAEAPATEAAATEAPAAEAPAAEAAK"
     gene            13187..14554
                     /locus_tag="WGR19_000393"
     CDS             13187..14554
                     /locus_tag="WGR19_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0797987.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELVGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14735..16765)
                     /locus_tag="WGR19_000394"
     CDS             complement(14735..16765)
                     /locus_tag="WGR19_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0797988.1"
                     /translation="MYYETRSKQASKQKFKKSFIISLFFSILYTSPLLAVDYVYDKTK
                     LTDDEITRLKKLRDKTSEYWKKETYLITGDNPKVPSVPALYPRTYQFENINNSKKISF
                     YDQKYTEDYLVGFARGLGVAKRNGDTEEPIRKYFKKCFNTELDDYSTCRAEQFRSHPL
                     VVNSHIFSLGPEIKNSHINSEILSVGNYIEWLRPTLNKLTGWQEQLYSGLDPFHYIDV
                     TDNSHVIGQTISLDQFRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAG
                     GARFRFAYGLKDKVPETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADK
                     HAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNDKNS
                     DIFDRTLPRKGLWLRVISGHLSQDVQGKTAPVEGNRKGIQLGGDVFSLQNHNYQLSVG
                     LMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQRFR
                     HRINTEDATERFTSKGITASIEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVNGK
                     FSDSENAHVNLLGSRQLQSRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEMDG
                     ERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGTHHQAKQGALNLQWTF"
     gene            complement(17131..17577)
                     /gene="smpB"
                     /locus_tag="WGR19_000395"
     CDS             complement(17131..17577)
                     /gene="smpB"
                     /locus_tag="WGR19_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0797989.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17632..18642)
                     /gene="waaF"
                     /locus_tag="WGR19_000396"
     CDS             complement(17632..18642)
                     /gene="waaF"
                     /locus_tag="WGR19_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0797990.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPAKHFAELGKHYSATGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18773..18848)
                     /locus_tag="WGR19_000397"
     tRNA            complement(18773..18848)
                     /locus_tag="WGR19_000397"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18813..18815),aa:Asn,seq:gtt)
     gene            complement(19039..19791)
                     /locus_tag="WGR19_000398"
     CDS             complement(19039..19791)
                     /locus_tag="WGR19_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0797991.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20102..20626
                     /locus_tag="WGR19_000399"
     CDS             20102..20626
                     /locus_tag="WGR19_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0797992.1"
                     /translation="MQTRILSAVLLAFSTTAFAGGAFTLQFDNPSKDGGFTQNQLLSA
                     PYGFGCSGDNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     AASLQLSRRASIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     TLCGTSSSVSTAAA"
     gene            20587..20766
                     /locus_tag="WGR19_000400"
     CDS             20587..20766
                     /locus_tag="WGR19_000400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0797993.1"
                     /translation="MRHFFIRQHRRRIIKRRAHPPGVRQLHRIAHCLRRPVLCGKPFY
                     GFLFESNPLRLIEAV"
     gene            complement(20763..20966)
                     /locus_tag="WGR19_000401"
     CDS             complement(20763..20966)
                     /locus_tag="WGR19_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEZ0797994.1"
                     /translation="MPSNYFHTRERLGALGGIVQHHTALYRSVAVYEPAVLIVRRGSK
                     KLRRVRRSPWQADRRLTLLIFPI"
     gene            complement(21338..22483)
                     /gene="dapE"
                     /locus_tag="WGR19_000402"
     CDS             complement(21338..22483)
                     /gene="dapE"
                     /locus_tag="WGR19_000402"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0797995.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAAICGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(22596..23216)
                     /locus_tag="WGR19_000403"
     CDS             complement(22596..23216)
                     /locus_tag="WGR19_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ0797996.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYRPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(23250..23759)
                     /locus_tag="WGR19_000404"
     CDS             complement(23250..23759)
                     /locus_tag="WGR19_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0797997.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQLVLQTIRQITRYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLHGN
                     WDAVAAEFAKLEADNAYIPDAEAFKRFVAGYDVHLAIEEPLFDMGKMFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(23854..24420)
                     /gene="azu"
                     /locus_tag="WGR19_000405"
     CDS             complement(23854..24420)
                     /gene="azu"
                     /locus_tag="WGR19_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0797998.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPK
                     ASMGHNLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPA
                     KLADGEYKFACTFPGHGALMNGKVTLVD"
     gene            complement(24701..25123)
                     /locus_tag="WGR19_000406"
     CDS             complement(24701..25123)
                     /locus_tag="WGR19_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0797999.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            25254..28004
                     /gene="secA"
                     /locus_tag="WGR19_000407"
     CDS             25254..28004
                     /gene="secA"
                     /locus_tag="WGR19_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0798000.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     FDVIGGLVDIYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNTIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28019..28171
                     /locus_tag="WGR19_000408"
     CDS             28019..28171
                     /locus_tag="WGR19_000408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798001.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            28149..29921
                     /locus_tag="WGR19_000409"
     CDS             28149..29921
                     /locus_tag="WGR19_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0798002.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30108..32036
                     /gene="rpoD"
                     /locus_tag="WGR19_000410"
     CDS             30108..32036
                     /gene="rpoD"
                     /locus_tag="WGR19_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0798003.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         8152 a         9078 c         7566 g         7339 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcac gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgattttc gcgccctctt ccgtacccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacaat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag acgaattttt cggatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cacgtcgccc tgtatcagta gcggacgcac acccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaatggg aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacagtt ttgccacacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc ggcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacctcagcc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc atttcactca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccaa ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaagacc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc cattaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gctttctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaacaaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 ggcgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagtttttta tccggctttc catgcttaaa cagcactgcc
     7981 tcttttcaga cattgacggt tgcagccgct tacctgaacc ttatagtgga ttaaatttaa
     8041 atcagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctcaagca
     8101 ccaagtaaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     8161 taaatttaat ccactataat gattgactat ttttttaatc atgttattat tttccataaa
     8221 atacatgaca ttaagatgtt tttccacaaa agatacacac accggcaaac accggctgtg
     8281 tttatctttt cttatgccta ttttttaatc atcgtatttt tatcttttaa tttcaatacg
     8341 caaactaact tatacacacg gttttcacat ctttggactg cttccgtgtg tatagtggat
     8401 attgccgttt tcctttctga caaaaatgcc gtctgagaac ttcagacggc atttgaaaca
     8461 tcggaatcag cggttttgtt cataccactc gataaacttg tctgctttga caaaacccag
     8521 cagcggctcg ctgcggctgc cgtcggagcg gacgacaaac acgcccggcg gcccgaacag
     8581 accgtattct ttcaacaacg cctgatgttc gggcatgttg gcggttacat cgatttggaa
     8641 aaagcgttcc atatcgactg cctgatgcac ttccggctga ttgagcgtgt aagccgccat
     8701 ttctttgcag gaaatgcacc agtcggcata aaaatccaaa acgacgggtt tgtcgggatg
     8761 ttctttcaac gccgtatcca tcgctgcctt cagcgcggca gtatcggcaa acatttttcc
     8821 gtgttccgaa gatttgcctg cttcagccgg cggattgagg gtcaggaaat ggtgcagcgc
     8881 ggtcgttttg ccgtttgcgc cctgccagcc gaaccacgcg ccgcctatca gcaatatgcc
     8941 gcccaatacg aatgccacag ctttcggacg gcgtttctgc ctgcgtccgt tgaccagcag
     9001 catacaggca ggaaccagca tcagcagcgt gtacagcgcg acgacgagat aatagggcaa
     9061 gtgcggcgtg gcgaggtaaa cggcgacggc aagcaggata aagccgaaag cgtatttgac
     9121 ggcattcatc caatcgcctg ccttaggcag gatatgcccg ccgaacgtgc cgatggcaat
     9181 cagcggaacg ccggtgccca acgccaaagt gtaaagtgcc aaaccgccta aaaccgcatc
     9241 gcccgtctga ccgatgtagc ccaaagcaaa tgccagcggc ggggcgacgc acggcccgac
     9301 aatcagcgcg gacaatatgc ccataataaa gacggaaacg attttaccgc ctgaaagcct
     9361 gctgctttga ttctgaaaat acgactgcac ggcgttggga agctggatgt tgaacagccc
     9421 gaacatagac agtgccaaga cgaccattaa agccgatgcc gccaacacca cccaagcctg
     9481 ctgcaaccat acggtcagca gtgcgcccgt cagtccggca acaatgccga ccagcgtata
     9541 agtcagagcc aaaccctgaa cataaacgac ggacagcaca aacgcccgcg ccttgcccgc
     9601 ctttttgtcg ccgaccacaa tactggaaac aatcggcaac aggggataca tacaggcggt
     9661 aaaactcaga cccaaaccgg cgagaaaaaa cgccaaaaga ttggcgttga gcgtatccca
     9721 agacagcttg aaacggctgt cgccgccctc atcccccttc gggggcggca gcaccccgct
     9781 gccgttttga gaggaaggct gcaaaaagcg gtctttggcg gattccggtt cgtcggtttg
     9841 cggatggtaa gtgccgttgc cggaaatatc aaactcggta tccacgggcg gatagcacac
     9901 gccggcttcg gcacagccct gataggtcaa aaccaattta tacggttcgc cgacagcctt
     9961 tgcataagga aaggcaacct gcgcttcgtg atggtaaacc gtctgcctgc cgaaaaactc
    10021 gtcttccttc tcttcaccct tgctgaaaga aggctgtccc aacaaatccg ccggatcggt
    10081 cttgccgacg attttcgcct gatacatata gtatccgtcg gcaatcctga aacggacgtt
    10141 cacaccgtcg tcggcaacgg caagctccgg cacgaatgcc ttttccggcg gcagcagatc
    10201 gttcgcatcc agcgcgaaag ctcgtccgca caacatcaaa aatacggcga acaggcaaat
    10261 cagttttttc ataatcgaat ccgtttcaga caaataattt gtctgcatta taaatggtaa
    10321 ggttgacggt gggatttaat ttatgtaaaa cccgccatta tccgaaccta tttccataaa
    10381 catcttatcg aacccgccat gtacgatgtc aatacccacg atgtccgccg ctttttcgcc
    10441 cgcgtgtggc agcagcggct caatccgctg caactgagcg cactggaaca gaaagccctc
    10501 cgcattgtcg aagcccatcc cgaataccac tgctatctcg aacgcatcga agaccatctg
    10561 gacaccgact ggctgcccga aaacggcgaa agcaacccct tcctgcatat gtcgctgcat
    10621 ttgtccgtcc aagaacaggc gggcatagac cagccgcacg gcatacgcgc aatccacgac
    10681 accctgtgcg ccaaacacgg ctggctggaa gccgaacacg aaatgatgga ggcactggcg
    10741 gaaacactgt ggacggcgca acgctacggc accggtttgg atgtcaattt ctacatgacc
    10801 cgactgcgca aactcatcgg cttgggtgca gaaaatcaag ccaggttgaa cccgcatgaa
    10861 atcgcctgac cataccaacc gcctgcaaaa tgccgtctga agcggaacaa cccctttcag
    10921 acggcattca ttttccccca atcatttcca caacgccttt ttcagcataa tcaaccaatc
    10981 cttcttatcc aaaacggggc gttgtgcaaa cacatcgtat cggcacgcgt ccagtttctg
    11041 caaaatcaac tgcgccccca acacaatcat acggagttcc aaaccgatac gcccattcag
    11101 ttcccttgcc aaaggcgaac ccgccttcag catacggaac gcacgccgac actcatacgc
    11161 catcagccgc tgaaacgccg catccgcccg tcctgccgcg atctgttcct cagaaacacc
    11221 gaatttcaac aaatcgtcct gcggaatata aaccctgcct ttttgccaat ccacagccac
    11281 atcctgccaa aaattcacca gttgcaaagc cgtacagatg ccgtcgcttt gcgccacgca
    11341 caccgcatcc gttttcccgt acaaagccag cataatgcgt ccgacagggt tggcggaacg
    11401 ccggcaataa tcggccagct cgccgaaatg cgcgtaccgc gttttgacca cgtcctgcga
    11461 aaacgccgag agcagatcat aaaacggctg caaatccaaa ccgaacggca caaccgcctc
    11521 ggcatccaat cgtgcaatca aaggatgcgc cgaccggccg cccgatgcca acacgtccaa
    11581 ctcgcgccgc aaagcatcca accccgccaa cctggcttca gacggcatac tgccctcgtc
    11641 cgccatatcg tccgccgtcc gtgcaaacgc gtacaccgca tgaaccggct tcctcaacct
    11701 gcgcggcaaa atcagcgaac cgacgggaaa attctcataa tgcccaaccg acataccttc
    11761 tccatccatc aaacaaaatg ccgtctgaaa cggaacaaac ccttttcaga cggcatcaga
    11821 tacctccaag ctgccggcaa tcaatggtgg tgatgaccgt gcggaccgtg gacatgaccg
    11881 tgtgcgattt cctcatcgga tgcatcgcgc acgctttcaa ccgtagcctt aaagcggatt
    11941 ttcatgcctg ccaaaggatg gttgccgtcc accaccgcct tgccgtcggc aacatcggtt
    12001 acacgataga cgacaacatc gccggtttca ggatcgtcgg cttcaaacat catgccgact
    12061 tcgacttcaa cagggaacac gcccgcatct tcaatacgga ccaactccgg atcctgctcg
    12121 ccgaacgcat cgtcgggcga cagcgccaca tcgaccgtat cgccggcatc cttaccgtgc
    12181 aacgcctctt ccaccaaagg gaaaatgccg tcgtaaccgc cgtgcagata cgcaatcggt
    12241 tcttcggttt tgtccaaaag ctgattgttg gcatcataca tctcataatg cagcgaaacc
    12301 acggaatttt tcacgatagc catatttgtc ctttcaggaa cagcagattg attacaggcg
    12361 cattctaaca caaccgccgc gccggccgat taccgttaac ctattcataa actgtacagc
    12421 acatatttca atgtaaattt ttgttatttt attgcggtgt aactttttta caacattctt
    12481 aaaaccattc cgacctgtct gccgactttc ccaatccgcc ttaataaatc atacaagata
    12541 ctgaaattat attaatctct ataatattta tccctatcga atttttaaca gcaaaaccgt
    12601 tttacaggat ttatcaatcc gcccgccaga aaacttttca ttcaaacctt tttcccatct
    12661 gtacgacatt gcaatccctt attccatagt gcataattac gcaaattcag cgatgaattt
    12721 ccaacccggt ttgtagtatg gtcgataaag acctatttgt ttcaataatt taaattggtt
    12781 ctaaaggtta ctaaaatgaa aaaatccctg tttgccgctg ctttgttgtc tttggttctg
    12841 gcagcctgcg gcggtgaaaa agccgctgaa gctcccgcta ccgaagcacc tgccgccgaa
    12901 gctcccgctg ctgaagctgc cgctactgaa gcacctgccg ccgaagctcc cgctactgaa
    12961 gcacctgccg ctgaagctcc cgctaccgaa gcacctgccg ccgaagctcc cgctactgaa
    13021 gctgccgcta ccgaagcacc tgccgctgaa gctcctgctg ccgaagctgc aaaataagca
    13081 ttttccgctt gcaaaaaagc aggatacgtt cagtatcctg cttttttgat ttttcagacg
    13141 gcatcagatt cccttcctca atcttctccc tacccttccg acaaacatgc ctgaccttca
    13201 taccgaattt tcccgactcc tgccggcaga tgaaattgcc gaactttctc cgacgctttt
    13261 aaaagaccag cgcaaccgct ttacgtctgc accagacatc attttgcagc cgcgcagcgt
    13321 tgaaagcgtg caaaccatta tgcgtttttg ttacgaacac cgtattccgg ttacgccgca
    13381 aggcggcaat accggtttgt gcggcgcggc agtatcggaa aacggcgtat tgctgaacct
    13441 ttccaaactc aaccgcatcc gcagcatcaa tttgtcagac aactgcataa ccgtcgaagc
    13501 aggttccgta ctccaaaccg tccaacaggc agccgaagcc tcaaacaggc tgttcccact
    13561 cagtctcgcc agcgaaggct cgtgccaaat cggcggcaac atcgcctgca atgccggagg
    13621 tttgaacgta ttgcgttacg gcacgatgcg cgacctggtt atcggtttgg aagtcgtcct
    13681 ccccaacggc gaactggttt cccatctcca tcccctgcat aaaaacacca ccggctacga
    13741 cctgcgccat ctgtttatcg gcagcgaagg cacactgggc atcatcactg ccgccacact
    13801 caagcttttt gcccgtccct cggacaaggc aacggcatgg gtcggcatac ccgacatcga
    13861 atccgccgtc cgcctgctga ccgaaaccca agctcacttt gccgaacgcc tatgcagttt
    13921 tgaactggtc ggccgttttg ccgccgagtt gtcttccgaa ttcagcaaat tccccctgcc
    13981 gacacattca gaatggcata ttttacttga gttgaccgac tcattacccg acagcaatct
    14041 tgatgatcgg cttgtcgaat ttctttataa aaaaggattt accgacagcg tattggcaca
    14101 aagcgaacaa gaacgtatcc atatgtgggc attgcgcgaa aacatctccg catcgcaacg
    14161 caaactgggc accagcatca aacacgatat tgccattcct atcggacgcg ttgccgactt
    14221 tgtccgccgg tgcgccaaag atttggaaca gaattttaaa ggcatacaaa tcgtctgctt
    14281 cggacatctt ggcgacggca gcctgcacta caatactttc ctgcccgaaa tcctcagcaa
    14341 tgaagtctat cgttacgaaa acgacatcaa cgacgcagtt tatcgcaacg ttcttgcctg
    14401 caacggcacg attgccgccg aacacggcat aggtatcatc aaaaaacagt ggctggacaa
    14461 agtacgcacg cctgccgaaa tcgccctgat gaaaagcatc aaacaacacc ttgatccata
    14521 taacattatg aatccgggca aactgcttcc gtaaccggca tttctgattt gcatacacaa
    14581 caaagaaagg gacaatagat ccgattgtcg gtttagcgcg agctcgtgag tgcggtaaaa
    14641 aattggtgga aatgacacga aaaatgaccg catttttaaa ataaaaaaat cggcagtgaa
    14701 tttccctgcc gattttattt tgttacaact taacttaaaa cgtccactgc aaattcaacg
    14761 caccttgttt agcttgatga tgtgtgcctg tttggcggtt gaatgttgct tgtaaagtta
    14821 agtgagattt gattttcact gctacaccta attggctctc aattgccgtc ttattgttta
    14881 tcactcgacg ctctccgtcc atttccacac cgaaaggttt gttgtggtaa agcgcgttca
    14941 cagcggcgaa aggttcaata gcgatatttt tatagagtga aaattgtgct ttagcttgaa
    15001 cgcccactcg gctttgtaat tggcgggagc caagtaaatt cacgtgggca ttttcgctat
    15061 cgctgaattt tccgtttacc cccaaataag tcaattgcgc ctgtggttgt aggtaaaaac
    15121 gaacacggtt gcccttttta gtgaagtgtt ctgccaataa cgcattgtaa cccgcttcaa
    15181 ttgaggcagt gatacctttt gaagtaaaac gttctgtagc atcttcagtg ttgatacggt
    15241 ggcggaaacg ttgatattgt acccagctat ccgcatacgc acctgtctgt ttgtcctgaa
    15301 gctgatgcca agtggcgtaa acgcctgcac caaagccttt cacatttccc gttgtaagat
    15361 tgtctgtatc tgggttgcgg aaagtactgc gttgttctgc ttgtccgccc attaagccaa
    15421 cggaaagttg atagttgtga ttttgcaatg agaaaacatc gccaccaagt tgtatgcctt
    15481 tacgatttcc ttctactggt gcggttttac cttgtacatc ttggcttaag tgaccactaa
    15541 ttacacgcaa ccacaagcct ttgcgtggta aagtgcggtc aaaaatatcg ctgtttttgt
    15601 cgttcaaacg taggttgaat aaagtattgg cggcttgagc ctgttgtgca taaatcgcaa
    15661 tatcatcgcg ttcttgcact ttggtaaaaa agccctctgg gcgttgttgt aaagaaagcg
    15721 tataaattcc cttttggtgt ttgccagaaa gacggaatgc gtgtttatct gctgtgccat
    15781 ttactttgat aatttgatgc ccgtcgaggc tttttaaatc gtctattgga ttttcgaaga
    15841 tgatgtcgga agtgccagtt atattttttt caaaagttaa aactggtgtt tcagggactt
    15901 tatcttttag accgtaagca aaacgaaaac gagctccgcc agcataatct tcttttacga
    15961 gtaaactttc acttttagta ttaaaacgga tgtctgcatt cgttgttttt aattccccaa
    16021 catcagaatc ccaacggggt tcccagagag aattttctaa tctgaattga tccaaactaa
    16081 ttgtttgtcc gataacgtgc gagttgtctg taacatcaat atagtggaat ggatctaaac
    16141 cagaatatag ttgttcttgc caaccagtaa gcttattaag tgttgggcga agccattcga
    16201 tataattacc tacagataaa atttcgctgt tgatatggct attttttatt tctggaccta
    16261 aggagaatat atgactgttt actacaagag gatgggatct aaattgttca gctctgcaag
    16321 tactataatc atctagctca gtgttaaaac atttcttaaa atatttccgt attggttctt
    16381 ctgtgtcccc atttcttttt gcaaccccta aacctcgggc gaagccaact aggtaatctt
    16441 cggtatattt ttgatcataa aaagaaatct tttttgagtt attgatgttt tcgaattggt
    16501 atgttctagg gtatagtgcg gggacggatg gaacttttgg attatccccg gttataagat
    16561 aagtttcttt tttccaatat tcactcgttt tatcgcgaag tttttttaag cgggtaattt
    16621 catcatcagt gagcttggtt ttgtcgtaaa cgtaatcaac agccaaaagc ggagaggtat
    16681 aaagaataga aaaaaataga cttataataa atgatttttt aaacttctgc ttgcttgctt
    16741 gcttgcttcg agtttcataa taaattttcc tttgtcaagt aaaaataaat ggggcgtgga
    16801 ttttagcata gaattgaaca aaaaatgtca tttagctcac atttttctct atttatttct
    16861 tatttattaa aagtaaacgt ttgctttttg ctattttgtc aagccagttt gaaaatgtgt
    16921 ataattgccc tcgttattca caaaaatttc aggaaaaatg accgcacttt acccttggtt
    16981 aatgccaatt tatcatcaaa ttgctcaaac ctttgacgaa ggtttggggc atcatgctgt
    17041 gctgattaaa gcggattctg gtttaggtgt agaacgttta cacatcaggc ggcagccttg
    17101 cccataccgt ctgaagcact gtttccacaa tcagcgcgta tgcttaatca accgctgttt
    17161 ctcgcgtttc caatccgcct ctttcatact ctgacgtttg tcgtgctgtt tcttaccttt
    17221 tgccaaaccg atttccatct tgatttttcc gcgcgaaaaa tgcaaatcca gcggcacgat
    17281 ggtgtagccg gcacgttcgg ttttgccgat taatttgttg atttccgact ggttcaacaa
    17341 gagcttgcgc ggacgtacgg catctggttt aatgtgtgtc gaggctgtgg gcaaagccgt
    17401 aatatggcag ccgaccagat aaaacgcgtc ttttttccaa tagatataac tctctttaag
    17461 ctgtacgcgc gcggcgcgga ttgctttgac ttcccagcct tccaagacca aaccggcttc
    17521 aatccggtct tcaatgaaaa aatcgtgaaa tgctttttta ttgttcgcaa tagccataaa
    17581 catcctatca atatccgccg tcagacggca taaacccgaa aacagaaccc atcataccgc
    17641 ctcttcaacc gcctgcacaa tcttctcggg atacagcctg ttgaggcagt cggtatgccc
    17701 cagcggacat tcccgcttaa aacagggcga acattccaag tgcaggctga cgattttcgc
    17761 cctgtcgctc aaaggcggcg tatgcgtcgg gcttgaagaa ccgtaaaccg ccaccacctt
    17821 cctgcccaat gccgccgcca agtgcatcag cccgctgtcg ttgcacacga ccgtgtccgc
    17881 caacgacaac aaatccattg cctgcgacaa atcggttttg ccgcacaaat tgacacacat
    17941 accgtctgaa aggcagttga tttcctcggc gatttcatca tctttttgcg aaccgaacag
    18001 ccaaacctgc caacccgtcg ccgaataatg tttgcccaac tcggcaaaat gctttgccgg
    18061 ccaacgcttt gccggcccga attccgcacc cggacaaaaa gccagaacag gctttccaag
    18121 attcaaaccg aaagtttcga cagaaatttc ccgccgccgt tcatcaatgg aaaactcggg
    18181 gaatcccgaa tgcccgtcaa aatcttcctg actcggatgc gcgagagccg tatatctgtc
    18241 caccatcaaa ggcagacgtt ccttatccag cctgcgtata tcgttcaata gaaaataacg
    18301 gctttcgccg acataacccg ttctttttcc gatgcccgtt gccagcgcga tgatggccga
    18361 tttcaaagaa ccgggcagca cgataacctg atcgtagccg cgccgtccca aatccctacc
    18421 gacccgccaa cggcgtttta actccaacgc accatgtccg aacggatttt caagaatctc
    18481 attcacttca ggcatacgct caaacaccgc catcgaccac ttcggtgcga acacatcaat
    18541 cgtgcaaccg ggatgaagtt ccttcaaacg gcggaacaag ggctgggtca tcacgcagtc
    18601 gcctatccaa ctgggagaaa taatcaggat tttgatggac ataacaagaa accgaaatca
    18661 gacaggcaga attttaccgc gaaaccgttg gaaaacctat cttaccgcat tccgaacgcc
    18721 ggacgtgcaa atatgaaaaa gcccgaacat tcaagttcgg gcttcaaaat tctggctccc
    18781 cgacctgggc tcgaaccagg gacctgcgga ttaacagtcc gtcgctctac cgactgagct
    18841 atcggggaat ggggcgtatt atagcgtcca gaaaaaatgt gtcaatcctt aattttggaa
    18901 aaatgggcga caaaacgaca agcatatgaa tcagaaagac attaagaccg atgccttaaa
    18961 aggattgccg ttgtatgaat ttccacagcc gtcatcacac catatttaag cccgatgagc
    19021 cgttctgccc tcccccgctt aaaacaatgc cgtctgaact tcgccgcgtt ccaaagccag
    19081 taaaaactgt ttgcggttca acccgcccgc gtagccggtc agtttgccgt cgctgccgat
    19141 gacgcggtgg cagggaatca ggacagacac cttgttctgc ccgttggcgg cggcaacggc
    19201 gcggacggct ttggggttgc ccaaacgctg cgcctgctcc ttgtagctgc gcgtttcgcc
    19261 gtaaggaatc gccaagagcg cgtcccatgc ctgcttttga aactcggtgc caatctgctc
    19321 caaaggcgtg gcaaaggttt tcagacgacc cttgaagtat aagtccaatt cctgccgcaa
    19381 aagttgcgtc cgctcatcct cccgaaacac aaaccgtccg cgcaaggctt tttggacggc
    19441 ggcaatttcc tgttccaaat gcttctgtcc gacaaactcc agcagacaca atcccttatt
    19501 tccgaacacc gccagcatct cgcccaaagg cgtggcaatg gcggcacaca ccagctcatt
    19561 caaactgtcg ggataacgcg cttccaacag acggatggct cggcggatac ggacatattc
    19621 ttcaggcgcg cagccgatat tgtcccaaaa atcccgatcg aactgcccgg cttcgcattc
    19681 cgtcaaattg ggatgcggca taacgccgca ctcacaaacc cgggtttcga gccaatagcg
    19741 gatttcatcc cattttgacg gcagattgtt taaggaaggc agggtaatca ttcgtttgct
    19801 ccgtatctct atcatggatt tggcggcaaa atccccaatt tttgccattc ccgcacgccg
    19861 gagcaggaac gggctatgac gtaaatcttg agggttaggt tgcggcaata cctaaatatt
    19921 cgatatttct aaagcctcag ggaaaggaat taaagacttc agacgacctg caaagggtcg
    19981 tctgaaacca cgatttttgc atttgcgcat tccggcacat catccaaccg tttcggcaca
    20041 ttcctgccgc cgttgacagc ctataatgaa tccacttatt catcaagcaa aggaatcatc
    20101 tatgcaaacc cgcatcctct ccgccgtact gctggctttt tcaaccactg cctttgccgg
    20161 gggcgcattc acgctgcaat tcgacaaccc gtccaaagac ggcggcttca cgcaaaacca
    20221 gcttttgagc gcgccttacg gctttggctg ttcgggcgac aatgcttcgc ccgcgctgtc
    20281 gtggaaaaat ccgcccgccg ggacaaaaag tttcgtcctg accgtttacg ataaagacgc
    20341 gccgaccgga ctgggctgga tgcactgggt ggtcgccgac attcccgccg atgtccgccg
    20401 ccgcaatgcg gcctcgctgc aattaagccg ccgcgccagc atcgccgacg accagtccgc
    20461 agccatatcg gcggtaatca gtttgcggat ttgccgcatc aggttgacgc cttcgtacac
    20521 ggcaaaaccg atgccgtcat gctgcaacca cgccaacacg ccgcaaagcg cggcctccgc
    20581 cacattgtgc ggcacttctt catccgtcag caccgccgcc gcataatcaa acgccgcgcc
    20641 catccgcccg gggtgcggca gcttcaccgc atcgcacact gcctgcgccg tcccgtattg
    20701 tgcggcaaac ctttctatgg cttcctgttc gaaagcaatc cattgcgcct gatagaggcc
    20761 gtctagatcg ggaatattaa taacgtcaaa cgtctgtccg cctgccaagg cgaccgcctc
    20821 acccgccgca gcttcttact tccgcgccgc acgataagca cagccggttc atataccgcc
    20881 acgctgcggt acaaggcggt atgatgttgc acgatgccgc ccaaagcacc caatcgttcg
    20941 cgcgtatgaa agtaatttga cggcactatt atctccaaca cgcagggccg cacataaggc
    21001 gcagccctgt ttaccctgat ttggaagggg ttacttccca aaaataaacc aaaatcccac
    21061 tgccccgaat ggccgggttt caaccatatt ccgtcggtgt gcctgcaact tgactccctc
    21121 ccctgtgggg gagggttggg gagagggtaa aacggggcag atacagacaa tatttccgtt
    21181 accgccccga tgccctctcc ctaaccctct cccacgggag agggaatgga ttgccgttga
    21241 aataaatcgc tctacataaa aaatcaatgt gttatctcaa acccacgtta ggtctaagca
    21301 aatggtcgga catccgtatc cggcaagcaa gctgctttca gacggcattt ccagccaaca
    21361 agcgcgccaa tatcccctca tacaccgcag acagcttcgg aatgtcgttt agccgcacgt
    21421 tttcgttgat ttggtggatg gtcgcattgg acgggcctaa ttcgatgagt tctttcgcaa
    21481 tggctttaat aaagcgtccg tccgaagtgc cgccggtggt ggacaattcg gcctctacgc
    21541 cgcagattgc ggcaatggct gcgcgtgcca catcggtcag ttttccggcc tgagtcagga
    21601 aaggttggcc tgagcaagac cactgcaaat catattgcac gccgtgtttg tccaaaatgg
    21661 cgtgaacgcg ttgtttcagc cctgcttcgg tggactcggt ggagaagcgg aaattgaatt
    21721 tgacgttcag ctcgcccgga atgacattgg tcgcgcctgt accgccgttg atattggaaa
    21781 tttgaaagct ggtcggcggg aagtatttgt tgccttcgtc ccagacttcc tgcgtcagct
    21841 ctaacaaggc cggggcaaaa gtatgcacgg gattgattgc caaatgcgga taggcaatat
    21901 ggccttgctt gcctttgacg gtcaggttgc ccgacagcga gccgcgccgg ccgtttttaa
    21961 tcatatcgcc caatttgtcc acggctgtcg gttcgccgac gatgcagtag tcaatcaact
    22021 cgccgcgcgc tttcaataca tcgacgactt tggtcgtgcc gtccaacgcg tcgccctctt
    22081 cgtcggaagt aatcagaagc gcaatgctgc cttggtggtc gggatgttcg gcaacgaagc
    22141 gttcacaggc ggtaacgaag caggcgatgc tggttttcat gtccgccgcg ccgcgcccgt
    22201 ataatcttcc gtcgcgttcg gtcggctcga acgggggcga atcccatttt tcaacagggc
    22261 ctgtcggtac aacgtcggta tgccctgcaa aacagacgac gggagctttc gtgccgcgtc
    22321 gcaaccagat gtttttggtg tcgccgaaat ggagttcttc agccgcaaaa ccgattttgt
    22381 gcaggcgttc ggcaaggagt ttttggcaat ccctgtcgtc aggggtaacg gatggtcggg
    22441 aaatcagctc tttggcaagc tctagggatt gagtttcggt catatttgtt cacttttgaa
    22501 attagaccgt ctgaaacgtt ctgaatgtga ttttcagacg gcatttaggt taggttggca
    22561 tacggggtgg gtattttacc catcagtctt ctgaatcatt tgccgtggca ggcttcgtaa
    22621 agcggcagca aatcttccac cgtttccgct atccatttcg cgacatcctg cctgcccaaa
    22681 tcgtcgcgtt cgatgtgttt gccgatgcag aaaaagtctt cgtcgttttg caactttcgg
    22741 tcggattcat tttgctgcgc gacggtgcgg tagtcgtcgt attcgctttc cgcaccgtgc
    22801 cacatatcga aagaagcgta tttttcggta tcaaaattat ccaaccaacg gttgtaatca
    22861 ggcagcgcga tgggggaaac atcggcttta tagcagtgcc aatccaagct gacactcaaa
    22921 cggcggcggt tgagcagtat cgacaaaatc gcggcggaat ttttatattg ttcgtatttg
    22981 aagtaggcaa agaaatgggc gcgaacctgc cagccgttgc accagcgttc gatgtgcggc
    23041 ggcgcaaacg gcgcacccaa ttcggcggca acctgctgaa tcagctgctg ccatatctgc
    23101 cagttttctt tatagtcagc cttgatttgc ggaatgcttt caggccggta ttttttaagc
    23161 tgggaaaatt ggaaaaacgg gatattgaac aaatcgcaac ttttcggggt cagcataata
    23221 tatccttgag acgattgttt cagacggcat tatttgcgcc ggcgcgccgc catgatttcg
    23281 ccgatttcgg tcagtttctc tttggggatg aacatcttgc ccatgtcgaa caagggttct
    23341 tcaatcgcca aatgcacatc atatcccgcc acaaagcgtt tgaacgcttc cgcatcggga
    23401 atataggcat tgtctgcttc gagtttggca aattcggcag ccacggcatc ccagttgcca
    23461 tgcaggctta catgttggcg taaaagctcg tccacgcttt cttgggcttg cggcgcatat
    23521 tgcagcagga gcgggaaaaa gttttcttct tcgtcctcat ggtgcagcgg cgcggcaacg
    23581 ttgaaatacc gggtgatttg gcggatggtt tgcaaaacaa gctgattgca gccgttttcg
    23641 gcgatgtagt ccgacagcat cgcgacttgg ccgcaaaaac ggcgtacttt gccgtggcag
    23701 gcatacagca tttcaatcgg ttcggcaaag gtaacgcttt gggtttcaaa cggattcatg
    23761 ttttcgttct caacgggcac ttttcaagca gtcattttat aataaaacag cctgcacaaa
    23821 gcaggctgtc cgtcttttga gactttaagc ggattaatcg accaaagtca ctttgccgtt
    23881 catcaaagca ccgtgacccg ggaaggtgca agcgaatttg tattcgccgt cagccagttt
    23941 ggcaggatct agagtcaggg aagactcttc gccgccgccg atcagtttgg tgtgggcaac
    24001 aacgcgcgca tcgtcaggtt tgacatagtc ggtatcggca gcacctacgc cgtctttaaa
    24061 tacgccgtcc atgtcttcag ctttggcaat cacaaggttg tgacccatgc tggctttggg
    24121 ttgcgtaccg gtatgtttca gagtgatggt aaactcttta catgctttgc tgacttggat
    24181 gtctttagtg ttgaactgca tattgtcgtt ggattcgaca gttgccgcac agttgccggc
    24241 agcaggggct tcggcagcat ctgcaggagc agcatctgca ggagcagctt cggcggcagg
    24301 tgcttcggaa gcgggtgctt cagcagcagg agttgcctcg gcagcaggcg cggcaggttc
    24361 ttgagagcag gcagccaaac cgataacggc ggcagaaatc agagccagat acgctttcat
    24421 aacaaatctc caatcgataa aataatattc ggttttacag aaatcaaagt gcaaccgcca
    24481 ttaacaaaac cttgaaaaag attccgccgc gttgcacaaa cagatgtttc ggagcgacat
    24541 tttgctacaa atttcatttg aaatcaaagc ctgtctgcaa gtttacaatc gtttacttta
    24601 aaaggggcaa ttttaccccg aacctatttc tttagtatta gacccattat cctttacttc
    24661 ttaatattaa cggatgttta cacaaattcc cgtatacatt ttatgcgcca tgccttctga
    24721 ccaagtttgc caatgcctcc gccaattcgg gatgccgttt ttccaacttt accgccgccg
    24781 aaccgaaact ctccagcgca gccttactca aatgcagggt attggttttc ggcggttttt
    24841 ccggtttcgg gaccagcctg accgaaacag agcgtatcga agcatcaagc cctgccaact
    24901 gcggcaatac cgacggtgca atcattttca agcgcgatgc cgccatattg tttgccgcca
    24961 aaaggacaag cctgccgtct tcgatacatg ccgtctgaaa atgcgggtgc aggttggcag
    25021 gcagcagttt tttcacggcg gcatccaacc gccgccactg tcccgcctgt ttcaaaagtc
    25081 cggaaagcag cgcgtcccgc ctgcccaact gttccaaatt cataaaacat acacccaaaa
    25141 agattgaaat accgcaaacg cgcctttatt tcagacggca ttagcacttt gcacaaacgc
    25201 ttgtgttaaa atcgcgtttt cgcccactat tatatcaggc gcaggaatta ttcatgctga
    25261 caaacattgc caagaaaatc ttcggcagcc gcaacgaccg cttgctgaaa caataccgta
    25321 aatccgttgc cagaatcaac gcgctcgaag aacagatgca agccctaagc gatgctgatc
    25381 tgcaagccaa aactgccgaa ttcaaacaac gcctcgccga cggtcagact ttggacggca
    25441 ttttgcccga agccttcgcc gtctgccgcg aagcgtcccg ccgcaccctc ggtatgcgcc
    25501 acttcgacgt gcagcttatc ggcggtatgg tgctgcacga cggcaaaatc gccgaaatgc
    25561 gtaccggcga aggcaaaacc ctggtcgcca ccctcgccgt ctatctcaac gcgctggccg
    25621 gcaaaggcgt acacgtcgtt accgtcaacg actacctcgc ctcacgcgat gcgggcatta
    25681 tggagccgct ctacaatttc ctcggcctta ccgtgggcgt gattatttca gatatgcagc
    25741 cgttcgaccg tcaaaacgcc tatgccgccg atatcaccta cggcaccaat aatgaattcg
    25801 gcttcgacta cctgcgcgac aatatggtta ccgaccaata cgacaaagtg cagcgcgaat
    25861 tgaattttgc cgttgtcgat gaagtggatt ccatcttgat tgacgaagcg cgcactccgc
    25921 tgattatctc cggccaggca gacgacaaca tccagttgta ccaaatcatg aacaccgtcc
    25981 cgccccacct cgtccgccaa gaaaccgaag aaggcgaagg cgactactgg gtagatgaaa
    26041 aggcacatca ggtcatcctg agcgaagcag gtcacgaaca cgccgagcaa atcctgaccc
    26101 aaatgggatt gctggcagaa aacgactcgc tctattccgc cgccaatatc gccctgatgc
    26161 accaccttat ggcggcattg cgcgcgcatt ccctcttcca caaagaccaa cattacgtca
    26221 tccaagacgg cgaaatcgtc atcgtggacg aattcaccgg ccggctgatg tccggccgcc
    26281 gctggtcgga gggtctgcat caagccgtcg aagccaaaga aggcgtggaa atcaaacgcg
    26341 aaaaccaaac ccttgcctcc atcaccttcc aaaactattt ccgcctgtac accaagctct
    26401 ccggcatgac cggtacggca gataccgagg ctttcgagtt ccaaagcatc tacaacctcg
    26461 aaaccgtcat catcccgacc aaccgccctg tacagcgcaa agacttcaac gaccagattt
    26521 tccgttccgc cgaagaaaaa ttcgaagccg tcgtcaaaga catcgaagaa tgccacaaac
    26581 gtgggcagcc cgtcctcgtc ggcaccacca gcatcgaaaa ctccgaactg gtctcccacc
    26641 ttctgcaaaa agccggactg ccgcacaacg tcctcaacgc caaagaacac gaacgcgaag
    26701 ccctgattgt cgcccaagcc ggcaaagtcg gcgcgattac cgttgccacc aatatggcgg
    26761 gacgcggtac ggacatcgtt ttaggcggca acctgaagca ccaaaccgat gccatccgtg
    26821 ccgacgaaac cttgagcgac gaagagaaac aggcacaaat cgccgcactc gaaaacggct
    26881 ggcaggcaga acacgataaa gtaatggaag caggcggcct gcacatcatc ggtacggaac
    26941 gccacgaaag ccgccgtatc gacaaccaat tgcgcggacg ttccggccgt cagggcgacc
    27001 ccggatccag ccgtttctac ctttcgtttg aagacccgct gctgcgctta ttcgcactcg
    27061 accgcgccgc cgccatcctc aaccgtctcg cccccgaacg cggcgtcgcc atcgagcaca
    27121 acctgctgac gcgccaaatc gaaggggcgc aacgcaaagt cgaaggcaga aacttcgata
    27181 tgcgcaaaca ggttctggaa tacgacgacg tcgccaacga gcaacgtaaa gtcatttaca
    27241 gccaacgcaa cgaaatttta accagcaaag acatcagcga cctgatgaag gaaatccgtt
    27301 ttgatgtcat cggcggactt gtagacatat atatgccgcc ggacagcatg gaagaacaat
    27361 gggacattcc gactctggaa aaccgtctgg ctgccgaatt cagactgcac gaagacattc
    27421 aatcctggct gaaagcagac aatacaattg acggtcaaga catcaaagag cgcctgatcg
    27481 aacgcatcga aaacgaatat gccgccaaaa ccgaactggt cggtaagcag gcaatggccg
    27541 atttcgagcg caacgtgatg ttgcaggtca tcgacaacca atggcgcgaa cacctcgccg
    27601 ctatggacta cctgcgacaa ggcatacacc tgcgcagcta tgcccagaaa aatccgaagc
    27661 aggaatacaa acgcgaagcc tttaccatgt tccaagacct gtggaacggc atcaaattcc
    27721 atattgcttc cctgcttact tcggttcaaa tcgaacaaaa ccctgtcgcg gcggttgaag
    27781 agcaacccat cggcaacatc cagtccatcc attccgaatc gcccgatatg gaagaacttt
    27841 tgggtcagtc gcaaaccgat ttggttaccg aggcctttaa tcccgatgga acggatttca
    27901 gccccgaagc cttggaagcg cggggacaaa tcgtccaccg caacgacccc tgcccctgcg
    27961 gcagcggttt gaaatacaaa caatgccacg gcaaactggc ttaagcgttt gaacgcaaat
    28021 gccgtctgaa catcccgctc ccgtttcaga cggcattttg cctgaaccgc cacatccgac
    28081 tgccattccg aaaaatcccg atttcgtacc gtccgtacca aaaacagaca tcccgtccgc
    28141 cccacatcat gattccatcc gacttcattg acgagctttt agccaaaacc gatattgtcg
    28201 atattatcga cgagcaggtt ccgctgaaaa aaggcggggc gaactatatg gcgtgttgcc
    28261 cgttccacaa ggaaaaaacg ccgtcgtttt cggtcagtcc aaccaagcag ttttaccatt
    28321 gtttcagttg cggggcgcat ggttcggcga ttggttttgt gatggagcat caggggctgt
    28381 cgtttccgga ggctgtgcag tttcttgccg accgtgtggg catggttgtg cctaaagtgc
    28441 gtgggcagaa cgataatccc gaagtccgtg ccgaacgtaa gaaaaaacag cagacgctgg
    28501 aagaaacgac ggcggcggcg gctgattttt acgcgcaaca gctaaaattc aatccggcgg
    28561 caaaagctta tttggacaag cgcggcttga gtgcagaagt tatcgcgcat tatggtttgg
    28621 gctacgcgcc cgacggctgg cagcctttgg cgcaagtgtt ccaaccgtat ccgaataccg
    28681 cgttggtgga tacgggtatg gtgattgaca atgaaggacg gcattacgac cgattccgtc
    28741 atcggattat gttccccatc cgcaatccgc gcgggcaggt catcggtttc ggcggcaggg
    28801 tgctggacga ctccaaaccc aaatatctaa actctcccga tacgcctttg tttgataagg
    28861 ggaaaaacct ttacggattg tatgaagggc gtgctgctgt caaggaagtg gggcggattc
    28921 tggtggtcga aggctatatg gacgtggtcg cgctggcaca gttcggcgtg ggctacggcg
    28981 tggcggcctt gggcacggca acaacagcgg aacacgtcaa aatcctgatg cgtcaggcgg
    29041 acagtattta tttctgtttc gacggcgaca gcgcggggcg caaagcggct tggcgcgcgc
    29101 tggaaaacgc gctgccgcag ttgaaggacg acaaatcgct gcattttttg ttcctgccgg
    29161 aagaacacga ccccgacagt tacatccgtg cctacggcaa agcgcaattt gaagacgcgc
    29221 tgttgaatca aagcaagcct ttgtcggagt atttctggga acacctttca gacggcattc
    29281 atctcaatac gcaggaaggc aaggcggaat tggtaaaaac cagttcgccg cttttggcgc
    29341 agattaccgc gccggcattg gcttatttgt taaaacaacg gcttagcgaa ctggtcggca
    29401 tcgaccccga caacctcgcg caactgctag gacaggaagc gccgaagcgg cacgtcaaac
    29461 aaaaaaacta caaactgcct ccgatttccg tcaaacagcc cgtcatgctg acgctggtac
    29521 agcggcaaat ccgcagcctc ttgataaatc cggattgggc tgcatatata gacctgcccg
    29581 attatctggc gttggacggt gatttcgcct gccttgccaa tcttgccgaa tcgattaaaa
    29641 accatgccgc cgtacccgaa accgctcagg ttttagagta tatgcgcggc tcgccttacg
    29701 aagaaacgat aacccgaatc ttccattcaa cgcaccaatc ggaagaaatg aacagcagca
    29761 gtgaagaaga ttgcgagaat ttccaaatcg gcatgaaaaa actgctcaat gagttaaaat
    29821 acagccaaat cgaagcattg aaacaaaaaa gcctgcaatc cggcttaaat gaaagcgaga
    29881 aaaaactttt gctgtcgttg ctgaccgcaa aacaaaattg accggcggat tccgccaccc
    29941 gtaaacccgt tatgccgtct gaaaagcact taccccggct gcaacaacga cacctgcaga
    30001 acacccatcc caaaaagcct tcagacggca tcagagtacc cgctctgcca cgccttcagg
    30061 tgcgttcaaa cgcaaaccgt cggcacctta ccaacagaaa gcagacaatg tccagaaacc
    30121 aaaatcacga agaatatcaa gacgacaccc gtccgttaag cattgaagag caacgcgcgc
    30181 gcctgcgtca gctcatcatc atgggtaaag aacgcggcta catcacctac tccgaaatca
    30241 acgacgccct gccagacgat atgtctgatg ccgaccaaat agacaatatc gtcagcatga
    30301 tttccggttt gggcatccaa gttaccgaac acgcccccga tgcggaagac atattgttaa
    30361 gcgacaatgc cgccgttacc gacgatgatg ccgtcgaaga agccgaggcc gccctttcca
    30421 gtgcagattc cgagttcggc agaaccaccg accccgtccg tatgtatatg cgcgaaatgg
    30481 ggcaggtcga cctgctgacc cgcgaagacg aaatcatcat cgcaaaaaaa attgaaaacg
    30541 ccctgaaaaa tatggttcag gccatctccg cctgcccggg atccattgcg gaaatcttag
    30601 aactcatcga aaaaatccgc aaagacgaaa tccgcgtcga cgaagtcgta gaagccatta
    30661 tcgacccgaa tgaagtattg ctcaacgaat tgggcttggg gcacttggaa accacagcgc
    30721 ccgagaaacc ttccaacgac aattcggacg aaaacgaaga cgacgaagaa tcggaagaag
    30781 atgcggatga aatctcggca gccaatctcg ccgaattgaa acaaaaagtc atcggccact
    30841 ttgcccaaat cgaaaaagac tacaaaaaaa tgattggccg tttggaaaaa caccacagcc
    30901 ggcacaaaga ctatctcgcc taccgcgacg cgattgccaa caaactgctg gaagtccgtt
    30961 tcgccacccg gcaaatcgac agcctcagca gcagcctgcg cgggaaagta gaaaacatcc
    31021 gcaaactcga acgcgaaatc cgcgacatct gcctcgaccg cgtccatatg gaacgcgact
    31081 acttcatcca aaacttcctg cccgaaatca ccaatctaga atggattgaa gaagaaatcg
    31141 ccaaaggcag ggtttggagc gacgcgctcg accgcttccg ccacgccatc ctcgaaaaac
    31201 aaaccgagtt ggcggatatg gaaaaagaaa cccgcatttc catcgaagag ttgaaagaaa
    31261 tcaacaaaaa tatggtgtcg agcgaaaaag aaaccgcagc cgccaaacag gaaatgattc
    31321 aggcaaactt gcgcctcgtg atttccatcg ccaaaaaata taccaaccgg ggcttacaat
    31381 tccttgatct gattcaggaa ggcaacatcg gtttgatgaa agcggtcgat aagttcgaat
    31441 accgcagagg ctataaattc tccacctacg caacctggtg gatccgccag gcaattacac
    31501 gctcgattgc cgatcaggcg cgtaccatcc gcattccggt acatatgatt gaaaccatca
    31561 acaagatgaa ccgcatctcg cgccaacacc ttcaagaaac cggcgaagaa cccgattccg
    31621 ccaaacttgc cgaactgatg cagatgcccg aagacaaaat ccgcaaaatc atgaaaatcg
    31681 ccaaagagcc gatttcgatg gaaaccccca tcggcgacga cgacgattcg cacttgggcg
    31741 acttcatcga agatgccaac aatgttgcgc cggccgatgc ggcaatgtac accagcctgc
    31801 acgaagtaac caaagaaatc ctcgaaagcc tgacaccgcg tgaggcaaaa gtcctgcgta
    31861 tgcgtttcgg catcgatatg aacaccgacc acacgctgga agaagtcggc agacagtttg
    31921 acgtaacgcg cgaacgcatc cgacaaatcg aggcaaaagc actccgcaag ctgcggcatc
    31981 cgacaagaag cgaccgtttg agaagtttct tggacagcga agacagcaag ctgtaaacca
    32041 aaaaaccgca ggtttcaaat acctgcggtt ttttcttaca caataaacaa cgcttccaca
    32101 tatcccacac tcctatcccg agacctttgc aaaat
//
LOCUS       DAWFJW010000011        30210 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFJW010000011 DAWFJW010000000
VERSION     DAWFJW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.011"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(249..896)
                     /locus_tag="WGR19_000411"
     CDS             complement(249..896)
                     /locus_tag="WGR19_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0798004.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANNLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(893..1261)
                     /locus_tag="WGR19_000412"
     CDS             complement(893..1261)
                     /locus_tag="WGR19_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0798005.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1271..1942)
                     /locus_tag="WGR19_000413"
     CDS             complement(1271..1942)
                     /locus_tag="WGR19_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0798006.1"
                     /translation="MKTVSTAVVLAAAAVSLPGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLIALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2107..2826)
                     /locus_tag="WGR19_000414"
     CDS             complement(2107..2826)
                     /locus_tag="WGR19_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0798007.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEARHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2910..4223)
                     /locus_tag="WGR19_000415"
     CDS             complement(2910..4223)
                     /locus_tag="WGR19_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ0798008.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLTRHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTIPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(4621..5493)
                     /gene="hpnD"
                     /locus_tag="WGR19_000416"
     CDS             complement(4621..5493)
                     /gene="hpnD"
                     /locus_tag="WGR19_000416"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0798009.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDGKTLQYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5664..7526
                     /gene="hscA"
                     /locus_tag="WGR19_000417"
     CDS             5664..7526
                     /gene="hscA"
                     /locus_tag="WGR19_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0798010.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKSDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7739..8362
                     /locus_tag="WGR19_000418"
     CDS             7739..8362
                     /locus_tag="WGR19_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0798011.1"
                     /translation="MFVNSLYKIGISDVSNFEGRMRHLENNGYANVAGLERILAVKTD
                     NYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSV
                     HERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGK
                     SGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            8399..9160
                     /locus_tag="WGR19_000419"
     CDS             8399..9160
                     /locus_tag="WGR19_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0798012.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            9215..9556
                     /gene="fdx"
                     /locus_tag="WGR19_000420"
     CDS             9215..9556
                     /gene="fdx"
                     /locus_tag="WGR19_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0798013.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9843..10430)
                     /locus_tag="WGR19_000421"
     CDS             complement(9843..10430)
                     /locus_tag="WGR19_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0798014.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            10643..10840
                     /gene="iscX"
                     /locus_tag="WGR19_000422"
     CDS             10643..10840
                     /gene="iscX"
                     /locus_tag="WGR19_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0798015.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10908..11309)
                     /locus_tag="WGR19_000423"
     CDS             complement(10908..11309)
                     /locus_tag="WGR19_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0798016.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11520..12479
                     /locus_tag="WGR19_000424"
     CDS             11520..12479
                     /locus_tag="WGR19_000424"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0798017.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12547..13857
                     /gene="tilS"
                     /locus_tag="WGR19_000425"
     CDS             12547..13857
                     /gene="tilS"
                     /locus_tag="WGR19_000425"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0798018.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            13931..14716
                     /locus_tag="WGR19_000426"
     CDS             13931..14716
                     /locus_tag="WGR19_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0798019.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEGKMPSEEVRRLRAVVPEGKVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGSADVWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLASYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            14726..14923
                     /locus_tag="WGR19_000427"
     CDS             14726..14923
                     /locus_tag="WGR19_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798020.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            15185..15538
                     /locus_tag="WGR19_000428"
     CDS             15185..15538
                     /locus_tag="WGR19_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798021.1"
                     /translation="MKNCKFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            15550..15855
                     /locus_tag="WGR19_000429"
     CDS             15550..15855
                     /locus_tag="WGR19_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798022.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            15917..17293
                     /gene="mpl"
                     /locus_tag="WGR19_000430"
     CDS             15917..17293
                     /gene="mpl"
                     /locus_tag="WGR19_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0798023.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVYEGFDTAQLDEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            17500..18552
                     /gene="bioB"
                     /locus_tag="WGR19_000431"
     CDS             17500..18552
                     /gene="bioB"
                     /locus_tag="WGR19_000431"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0798024.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18630..19265)
                     /locus_tag="WGR19_000432"
     CDS             complement(18630..19265)
                     /locus_tag="WGR19_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0798025.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGSHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQIGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            19588..19848
                     /locus_tag="WGR19_000433"
     CDS             19588..19848
                     /locus_tag="WGR19_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0798026.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19911..21770)
                     /gene="ilvD"
                     /locus_tag="WGR19_000434"
     CDS             complement(19911..21770)
                     /gene="ilvD"
                     /locus_tag="WGR19_000434"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0798027.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHALTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21943..23712)
                     /gene="cysI"
                     /locus_tag="WGR19_000435"
     CDS             complement(21943..23712)
                     /gene="cysI"
                     /locus_tag="WGR19_000435"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0798028.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23739..25553)
                     /locus_tag="WGR19_000436"
     CDS             complement(23739..25553)
                     /locus_tag="WGR19_000436"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0798029.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKASDSLEAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDRRFEELGAKRLLERVDADLDFAAAADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25550..26836)
                     /locus_tag="WGR19_000437"
     CDS             complement(25550..26836)
                     /locus_tag="WGR19_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0798030.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLVIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26875..27798)
                     /gene="cysD"
                     /locus_tag="WGR19_000438"
     CDS             complement(26875..27798)
                     /gene="cysD"
                     /locus_tag="WGR19_000438"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0798031.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27836..28570)
                     /locus_tag="WGR19_000439"
     CDS             complement(27836..28570)
                     /locus_tag="WGR19_000439"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0798032.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(28586..30037)
                     /gene="cobA"
                     /locus_tag="WGR19_000440"
     CDS             complement(28586..30037)
                     /gene="cobA"
                     /locus_tag="WGR19_000440"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0798033.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLSELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
BASE COUNT         6499 a         7979 c         8685 g         7047 t
ORIGIN      
        1 tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
       61 ccactatacc atacaaccac gccggaatta agtttaaatt tgaataaaag gttcgggttc
      121 tgcaaaatac agaacccgaa ccttgttcgg atattgaaac cggctgcccg attttgggcg
      181 gtacggcttg caagtatcaa gattcgcata tgccgtctga agttcggaga ggttcagacg
      241 gcatatgctt atttgggctg ctcttcgacg aatctcggac ctttcaagat gccgttgtga
      301 gaatagggcg acagcaggtt gtatgcggcg gttttggaaa cttgataacc gcggtcggtc
      361 aggttgttgg caatctgatt gaccactgcg ctgaccaaag cccccaacag gccgctgttg
      421 ctgttgttgc tgccttcgcg gatgctggcc gaacccgacc acaactcttt tccgttacgg
      481 gaatcgacca gccgtgcttt ggcggatacg gtcgtcacgc tgtctaaaat ttgatatgaa
      541 gtgccgtatt cggtaaccgt aatgtacaaa accgcatcat tgccgaaaat ctgatgcagt
      601 ttttccggtc ggacggcgtg aatatcggcg gcattggtca agccgttttc tttgaaggtt
      661 tcctccacga ctgcggcggg gaagacgtaa tagccggctt cggaaagcgg agcggcggtc
      721 gaagccagta caccccatgt tccgttgaca tcgggcgatt cgttcagcgg cggaaccacc
      781 aaaattgaag ccggtttgct ttccttgaat gacgtgtagt cgaaatcggg cgctttttga
      841 acttggcagg cagacagcgc caacacggcg gcaagcccta aaatcaaagg tttcatcgct
      901 tgcctccttt accggttttc atcaggaagt ccataaatac gcccgattcg ggaaacagcc
      961 ttttctcttc ttcaaactgg cggaacgcgc cctctttgtc tcccgaacgg gaaagcagca
     1021 gtcccaaatg ggcgtgcgca cccggggcgg cattcatttt tttgttgccg gcttccacaa
     1081 agtatttttc catcttttcg gtctgcttgc ccaacgaagt gtcgtcgttt ttcaaacctt
     1141 catagacggt atcgggatag ccgccgtaat aatacaggga tttttgcccg ttgccgccgc
     1201 aggcggtcag agccaagacc gccgcacaca gcgacaaacg gctcaaggtt ttcggattca
     1261 tcatttctcc ttaacggttg ggttgccatg cgccgttgtc aacagcctga accaggctgt
     1321 tgacggcttc gcggattgcc aagtctaaaa ctttgccgtt caaagtcgca tcgtagccgg
     1381 aagtgccgcc gaaaccgatg atttcacggt tggaaagtgc gtattcgccc gcgccctgtg
     1441 cggaatagac gatttcggaa gtattgacgt tgacgatatt cagagccact tttgcatagg
     1501 cgatttgcga tttgccgcga cccaaaatgc cgaagagctg atgatcgccg acatctctgc
     1561 gtccgaattc ggttacatcg ccggtaacga cataatctgc gcctttcagg ttatgcgctt
     1621 tgccggaaat gccggattcc tgttttaatg cgatcaaatt ggtgcggttc agtacgttga
     1681 agcggttggt ctgttgcagg tgcgttacca gaatggtttt tgcctggctg cccaaacggt
     1741 cttccccgtc ggagaaaatg cctttttgga agctggagcg gttgtcgaat gttccgacgg
     1801 aaatcggggt acgaacacca tgatattgcg tattgtagga ggcgactttt gctacctcga
     1861 gactgcgtga ggattcggtc gcacagccgg gcagtgaaac ggcagcggcg gcaaggacaa
     1921 cggcggtgga aacggttttc ataaaattta ccctaaggtc aagttaagga aataacgagt
     1981 tatcattatt gtccttatgt aaatttaagt caaggtgttt gtctgtgtgg gacggatgcg
     2041 cgcggaaggt acggatattt ttcaaacgga atcgcggtgc gggcgggatg cctgcctttt
     2101 ccggatttag aggtaaacga tttcgccact ccgccctttg ctttcggcac ttgcccacca
     2161 gacaaatgcg ggcagcacgt ccccgtagct tttgcgttcg cttttggctt cgcccggatg
     2221 ggatttgatg cgttgcgggg aattgatggg gccggggacg aggacgttgg cgcgcaggtt
     2281 gccgaagcgt tcccattcgt cggcggcgac tttgcacagg tagttcaacg cggctttgga
     2341 cgcgccgaag ccgccccagt aggctttggg tgtttcgccg tggctttcgc cgacgaagat
     2401 gacggacgcg tcgggcgact gcttcagcag cgggaacagg gcgcgggtca gccccatagg
     2461 tgcgacggtg ttgatgcggt attggttgac ccattcggcg acggtttgga aatccagcgg
     2521 cgagagggcg taaaaatagc cggcgcagtg gacgatgccg tccagtttgc cttgcgtggc
     2581 ttcggcaatg gtggcggcga actgattgaa ttctttttct tccgcactca tcaggtcgaa
     2641 acggatggcg aagggttcgg ggtgtctggc ttcgacaatc gcgtcataga ctttttccag
     2701 ttttttctga tggcgtgcca ccagaatcac ggttgcgcct gccgccgcat aggctttggc
     2761 gacctgttcg cccagacctt gcgatgcgcc ggtaattaag atggttttgt cggacagtgt
     2821 cgccatactt tttccttttt ggtggtcggt cgggggtatt ttagcgtttt gccgcacctt
     2881 gtaaagcgtc ccgataccgg ccggcgtttt cagacggcat cgctcaggct ttgcaggcag
     2941 gcttccgccg acgcgaaacc tgattgtacg gcggcttcga gcgtggcggg gtagtctggg
     3001 tggaggtagt cgccggcggg gaagatgcgg tgccggtgca accacgacaa atccggcggc
     3061 ggggcatcgg ctgcggttgt ggcgcgtttt tcggtgatga cgcgcacggc ttcgggttcg
     3121 cccaaatgcg gaaggatgcg tttgaggtcg gcgtgaactt tgtccgccca cgctcggttt
     3181 gtaaacgcgc cgacgcggtc ggaaacgctg atgacggcgg acacttcgtt ttcaggcagt
     3241 ccgagcctgc cccggcaaag cagccattgt gccgtgccgt cggcaaggcc ggtcagcggg
     3301 gcgggcaggc ggacgggttc ggcgtagcgc agatagacgg tggtgatggc gtgatagcga
     3361 aggttttgat atgccgtctg aacgtgttcg ggcgtgcctt cgggcaggag cgcggcggcg
     3421 tggtagggcg cggtggcggg gacggcggca tcgaaagctt cgccgttgac gagcactttc
     3481 ccgtccggga tggtgttcag acggcatacg cgcgtttcga ggcggatgtc cgcgccgagc
     3541 cgttgaagct ccgccaaggc gggttcggcg acgattgcgc ccaaatcctg cttgggtagg
     3601 agatagtcgc tgccggattt tttcgtcagc acgccgtcgg acaaaacgtt gcacaacacg
     3661 cgcaggcttg cggtttccaa aggcgtgttg agcgcgcccc acaccagcgg ctgccaaaac
     3721 tgcatcacgg cggcacgcgg cacgttccgc tgtttcagcc attgtgcaac tgtcgtgtcg
     3781 ggctgtccga ggcgtgcgga cttctgcaaa tcggacatat cggcaagcag tttggctttg
     3841 aatgcggacg gtacgcgccg ggcaagcagc acgccgccca aaatatgcag cggtgcgggc
     3901 agggggaggg cgcggaactg caaaccgccg tgcatatgcc agtgcagcgg tacgcgcaaa
     3961 aaggcggcac ggggatccga accgatggtt ttcatcaggc gcaacacgcc ccggtatgcg
     4021 ccgagcaaaa tgtgctgccc gttgtccaaa aaaccgaaac cgtcggtatt tccggccagt
     4081 gtgcgcgccc tgccgcccgc ctgccggccg gcttcaaaca gggtaacgtc ggcgtgccgc
     4141 gtcaaggtga cggcggcgga caagccggcc cagcctgcgc cgatgacggc gattttcggg
     4201 cgcggatgcg gcgtgttcat catttattcc tccaatggtt ttgcagccgt atctatttcc
     4261 gtttccgaaa atgacggtaa aaaaggatac aggctaaaaa taaaggcagc agaaagcgcg
     4321 catagggata ccacggatgg tctgcatgcc agtatccgta ccgtccctgt ggaacggtat
     4381 catagcggta attgtcgggg aaaaagttga tccaaaccgt tactgccagc cataaggcta
     4441 tatccctgac tgctttcatt tacttctgct cctgtttaaa ttcccagcaa ttcgatttca
     4501 aagcgcgaac gccaacggga ttgcgcggtt acgatgcaga ctttcagacg atggttgaaa
     4561 ccccgtccgg cggggctgtg ggaatcgggt ttgcctgacg ggcggcgggc ggtatgcgcc
     4621 ttatgcccgt tccggcgtgc cggggcgcgg tttgaatccg aataaccagg ttttcagggc
     4681 aatgcgtttt ttgcgcggcg aagggagggc gattttgtat ttgaggacgt tttgcgcgcc
     4741 gtcccggtcg atttcgttca atagctcgta ataaaccgcc gccataacca gtccgacttt
     4801 ttgggctttt ttatcggcag ccggcagcag cgatacggct tcgcggtagg tttcacgggc
     4861 gcgtttgatt tggaacgcca tcaattcggc aaaattgccc gtcgggctgc attgcaaaat
     4921 cacgcttgcg gggacatcaa accgctgcat ttcctccatc ggcaggtaaa tccgtcccct
     4981 gcgcgcgtct tcgccgacat cgcggatgat gttggtcagt tgcagcgcaa gtcccatctt
     5041 gtcggcgtat tgcagcgttt tgccgtctga aaaccccaaa atccgcgcaa tcaggcagcc
     5101 gactacgcct gcgacgcggt ggcaatacag tttcaattcc tcaaaactgc cgtaacgcgc
     5161 ctgaaccaaa tccatctgca tcccgtcgat taaggcttcc agttcatatt tcggcagctt
     5221 gaaggtttcc ttaacctgcc gcaaggcctg attgacgggg tgttccggca tcgcgccgcc
     5281 gaataccttg tccaaatcgc cgcgccacca gttcaatgtt gcctgtgcaa catcggggtt
     5341 ggaacattcg tcaaccacat cgtccaattc gcggcaaaag gcatataaaa tcgttaccgc
     5401 atcccgtttt tcctgagtca ggaaacggaa gcccgataaa aaactggagc ggctttcttc
     5461 tgctttttga cggcaatagt cgagtccttt cacgatttat attcctaatg atgggcggga
     5521 aaggcggatt ttatcggcat ttggcggtag agggcaattt cggcggcacg acccaatcct
     5581 tagcggtttg ctcaactatc ggcgcaaatt ctgttaaaat gccgctttcc ttcctttaca
     5641 caccgcaccg acaggcagaa tttatggctc ttttgcagat ttcagaaccg ggtatgtccg
     5701 ccgccccgca ccggcaccgt ttggcggcag gcatcgattt gggtacgacc aacagcttgg
     5761 tcgccaccgt ccgcagcggc agtgccgcct gcctgcccga tgccgacggg cgcgttaccc
     5821 tgccttccgt cgtccgttat ttggaaaacg gcggcattga agtcggcaaa accgccctgt
     5881 ccgcccaaaa atccgacccg ctgaacaccg tcagctccgc caaacgcctt atcgggcgga
     5941 cgcttgccga tctgcatcaa aatacgcatt acctgcctta ccgtttcggc gacaatcaac
     6001 gcgttatcga actgcatacg cggcaggggg tgaaaacgcc tgtcgaagtg tcggcggaaa
     6061 tcctcaaaac ccttaaatcg cgcgccgaag aaaccttggg cggcgatttg gtcggcgtgg
     6121 tgattaccgt ccccgcctat ttcgacgatg cccaacgcca ggccaccaaa gatgccgcgc
     6181 gtctggcggg tttgaacgta ttgcgcctgc tcaacgaacc caccgccgcc gcaatcgcct
     6241 acgggctgga caacgcctcg gaaggcacgt ttgtcgtgta cgacttaggg ggcggcacat
     6301 tcgacgtatc cgtattgcaa ctgaccaaag gactgtttga agtcaaagcc accggcggca
     6361 acagcgcgtt gggcggcgac gatttcgacc accgcctgtt ctgccgcctg ctcgaacaaa
     6421 acggactctc ccaactcaac gaacaagaca gccaactcct gctttcgctc gtccgcgccg
     6481 ccaaagaaca attaaccacg caaaccgaag cgcgcattca ggcgacgctt tcagacggca
     6541 tggcaatcga cacaagcatc agtcgcgccg agttccacaa cctgacgcag catttggtga
     6601 tgaaaacgct cgaaccggtc acacaggcgt tgaaagatgc cggtgtcggt aaaaacgaag
     6661 tcaaaggcgt gattatggtc ggcggttcga cccgtatgct gcacgtccaa caggcagtcg
     6721 ccaccttctt cggacaaacc ccgctgaaca acctcaatcc cgacgaagtc gtcgcgctcg
     6781 gcgccgccat acaggcaaac gtcctcgcag gcaacaaaac cgacggcgaa tggctgctgc
     6841 tggacgttac acctttgtcg ctcggtttgg aaacctacgg cggcttggcg gaaaaaatca
     6901 tcccgcgcaa ttccaccatc cccaccgcgc gcgcgcagga ctttaccacc ttcaaagacg
     6961 gtcaaaccgc gatgacgata cacgtcgtgc aaggcgagcg cgagctcgtt tctgactgcc
     7021 gcagccttgc caaattcacc ctgcgcggca ttccaccgat ggcggcgggt gcggcgcgta
     7081 tccgcgtaac cttccaaatc gatgcggacg ggctgctgtc cgtttccgcc caagaacaaa
     7141 gcaccggcgt acaggcgcaa atcgaagtca aaccttccta cggcttggac gacggcgcga
     7201 ttacccgaat gcttaaagac agtatggaca acgccgccga agatatggcg gcacgcgccc
     7261 gcgccgaagc cgtagtcgaa gccgaaagcc tgaccgatgc cgtcaacgcc gccctcgagt
     7321 tggacagcga tttactggat gccaaagaat tgcaacagat ccggcaaggc atcgccgatt
     7381 tgcaaggccg tctgaaagac ggaaaagccg aagacatccg ctccgccgtc gccaaactca
     7441 gccgcagcac cgacaacttc gccgccaaac gcatgaaccg caatatccaa cgcgcattga
     7501 caggccagag tgtcgataat atttgatact taaacggttt cagacggcat agatataacc
     7561 tgatgccgtc tgaaggctcg aaaacacttg aaaaacatcg atatggaaaa gtcaggcatt
     7621 gtctattcga tgaaaaccgt catcaagggc gtgtatagtg aattaaattt aaaccggtac
     7681 agcgttggct cgccttgccg tactatttgt actgtctgcg gctcgccgcc ttgtcctgat
     7741 ttttgttaat tcactatata aaatcggcat ttcggatgta agcaattttg aaggcagaat
     7801 gcgccatttg gaaaacaacg gctatgcgaa cgttgccgga ttggaacgca tcctcgccgt
     7861 caaaaccgac aattacaaag aaaaagaaaa cctgctccat gaaattttca gcaaaagcag
     7921 gataggcgat accgaattgt tcgccgtgga cgaaaacctt gtaaaacgtt tgtttttatc
     7981 gcttcgcggc gaaatcgtgt ttccgaaaaa cgaaacggcg gaatcggaat ttgaaaaaag
     8041 cgtccacgaa cgcaggcagg aagggaatgc cgggtccggc cgcaaacaac tgcttgattt
     8101 ggtacggcgc ggacaccggg aataccctta cgcgctgccc cggcttttgg cgggcgcggc
     8161 atcctacaag ccgaaaaaat cgaaaatccg cctttttaaa gaagcatatt tcggcaaaag
     8221 cggcacgagg ctgaccgacg aaattgcaga cggcatccat atttacacct gtttttcgcg
     8281 ggcggatttg gaaaaagcct attccgaata tttggaactt ttcaaatccg aatcggatgc
     8341 cgaaggcaga aagccgcagt aaggtgcaaa cagataccgt acacgttgag gagcagatat
     8401 gatgggcgat tccgtcattt attatgtaga acaggcagac aaaccggtaa accgtgccgg
     8461 cgaacgcgcc cgtaaaacat tcaaatattt ttggcgcgag cttttttggg aacgccgccg
     8521 catcattccc gccttagatt ttgccatggt caaagcccct tttttccaag acggcgaaga
     8581 cggcgaaatt tgcgaacata tgtggatcga cgatatttat tttgacggtc tttacattta
     8641 tggcgtgctg aacaatgaac ccggcgaact gaccaatgtc gaacaaggcg aaagcgtttg
     8701 cgttccggtt gacgacatca gcgactggat gttcgtgtgc aacggcattc cctacggcgg
     8761 ctttaccata caggcaatgc gtggacagat gacggaagag gagcgtaccg aacacgatgc
     8821 cgcatgggga atcgatttcg gcgatcccgg gcaggtattg ctggtgtatg aagaaaaaga
     8881 acatcccgaa aatctggaag agcatccgat gtgccggaac tgtattgacg attttcggca
     8941 acagttgtcc caaaatccgg attttctgca tgagcaggac gaagacggct atacgccgct
     9001 tcatcatgaa gccatcgcag gaaatgcact tatggttcaa gccatgcttg aatacggcgc
     9061 aaatcctgcc tcaaagacat cggaaggcta taccgccctc gattttgccc gcctggcggg
     9121 ctggcaaaat gttgccgacc tgctcgaacc gcgacattag gcggacagtt ttccgaaaac
     9181 gaacacaaac actttttaca gaaagacaat aaaaatgccc aaaatcaccg tacttccaca
     9241 cacgacatta tgccccgaag gcgcggttat cgaagacgcg cccgaaggca aaaccgtcct
     9301 tgacgtgctg ctcgaccatg atatcgaagt cgatcacgcc tgcgaaaaat cctgcgcctg
     9361 cacaacctgc cacgtgatta tccgcaaagg tttcgacagc ctggaagagc cgaccgaatt
     9421 ggaagaagac ctgctcgatc aggcttgggg tttggaagcc gattcgcgcc tgagttgtca
     9481 ggcggttgtc gccggcgagg atttgattgt ggaaatcccc aaatacacca tcaaccacgc
     9541 gcgagaagaa cactgaaaac aggccgtctg aagccggcac gcttcagacg gcattgttgc
     9601 gcggataagg cgcaatcgcc cgaaaacagg cgttcgtaca ggcggaactt tcgattctat
     9661 agtgaattaa caaaaatcag gacaaggcgg cgagccgcag acagtaccaa tagtacggca
     9721 aggcgaggca acgctgtacc ggtttaaatt taattcacta tatattgatt tttatcggtt
     9781 ttctgacgga acggattggt gcggcatccg aggcggatta ctcgggcgcg atgcacgggt
     9841 atttatcggt tttgcagccg gaaaaaccgc cggcgggtta ctttcagggc ggcggtcggg
     9901 cggtatgccg gatagcgttc cactttcgct tctatattga ttttgattgg ttttctgacg
     9961 gaatgatccg atgcggcatc cgggacggct tgtgtttctt cctgcccgcc tgccggatat
    10021 tcctgtactt gcacgaaacc gaaagaggtg gcggtgcggc ggacaagctc gagatagcgt
    10081 ccttcaagct ccggacaggc ggcggacacg ctttctgccg taaccgtgaa gccgccgccc
    10141 gacccggcaa ggcgttccgc aatctgcgtg tagatgcgcc aacgttgggc gcgcaacatt
    10201 gccgcatcgc aaccggcagc cgctttatcc aaggcaatca gaccgcgcct gctccggtag
    10261 tgaaggcaaa ccgttactct ggagaggata tgttgcccgt ccaatatttg atacagtttg
    10321 cgtatcagca gaatcaggcg gtcaaactcc tccatctccg acagggaatc aggatggtcg
    10381 gaagcggtat aaaccagttt ggacaatttt gcggcaaaca tattgttcat caatcttcct
    10441 tgtcggttga aaccccgctc ttcggggcgg tagaatcaga tttgtttggg agggacaact
    10501 cctcccagat caggacgaca cataggctgg tgcttgatgt gttgtccggc gagttgaaac
    10561 attcagccat cctcaaggtg cggcagtttt gccgaaacat attctacacg gcttcaatgc
    10621 cggacgataa aaggaaattc atatgaaatg gaccgacacc cggcgtatcg ccgaagaact
    10681 ctatgacctg cacggcgaaa ccatcgatcc cagaaccgtg cgctttaccc aactgcgcga
    10741 cctgattatg gcattgcccg aatttgacga cgaccccgcc cgttgcggcg aacgcatcct
    10801 cgaagccgtg cagcaggcat ggatagacga ggcggaataa gtttcgggaa tgccgtctga
    10861 aatgcggcgg tacgcggttc gtgcttctgt ttgcagcggg aatggtttta ccagtctcct
    10921 tttttcagcc tgtccagttg gcggcggtcg cgcttggtcg gtctgccgtc gggataggcg
    10981 gaagtgatgc ggctgaattg gtcgagctgt ttgcgctctt ccctcaatgt tgccgttttc
    11041 gcgtcctctt catacagaag ccgcgcctcg gatgccgggc ggcgttggtg gttcaaacct
    11101 ttaaccttga ttttataggg aagggaattg agcgtcaggt cgataatatc gccgatgtct
    11161 atggttttac tgtttttgac cttcgagccg tttacttgaa ccctacccag ttcgatgtgc
    11221 ttttgcgcaa gggaacgggt cttgaaaaaa cgtgccgccc aaagccattt gtccagccgc
    11281 atggcggaag aatcgtgctt gtctttcata cgattttgtt tgaaataatt gaatttgttt
    11341 caagtttagc ataagatacg ccgcctataa ctagtatata tgcactaatc cactgttttc
    11401 catgctgtcc gaacacaaaa agagggtgtg gaaaagccgt tttggacaat aaattaactg
    11461 cggaatatgc acaaatagcg tatgatagcg gcagaatctg ttgatgagag cttcattcta
    11521 tgaaacctgt ttttttggat tttgaacaac ccatagccga actgaccaac aaaatcgatg
    11581 agctgcgttt cgtccaagac gagtctgccg tcgatatttc ggacgaaata caccgtttgc
    11641 agaaaaaaag caacgacctg accaaatcga ttttcagcaa acttacgccc gcccaggttt
    11701 cacaggtttc ccggcatccg cagcgtccct atactttgga ttacattgag gcactgttta
    11761 ctgattttga agagctgcac ggcgaccgcc actttgccga cgatcatgcg attgtcggcg
    11821 gattggcgcg tttcaacgga caaagcgtga tggtcgtcgg gcatcagaaa gggcgcgaca
    11881 ccaaagaaaa aatccgccgc aacttcggta tgccgcgccc cgagggctac cgcaaagccc
    11941 tgcgcctgat gaaaacggca gaaaaattcg gcttgcccgt catgaccttt atcgatacac
    12001 cgggcgcgta tcccggcatc ggcgcggaag aacgcgggca gtcggaagcc atcggcaaaa
    12061 acctgtacga actgacacgc ctgcgcgttc ctgttttgtg taccgtcatc ggcgaaggcg
    12121 gttcgggcgg tgcgttggcg gtcgccgtgg gcgattacgt caatatgctg caatactcga
    12181 cctattccgt tatttccccc gaaggctgcg cttcgatttt gtggaaaacc gccgaaaagg
    12241 cggcggatgc ggctcaggct ttgggcatta ctgctgaccg cctgcaaaag ctggacttgg
    12301 tcgataccgt catcaaagaa ccattgggcg gcgcgcatcg ggattttggg caaaccatga
    12361 aaaacgtgaa agccgttttg gaaaaacaac tgcacgaagc gcaaagcatc ccgcttgccg
    12421 atttgctttc gcgccgtttc gaccgcatta tggcttacgg caaattttcg gaacaataat
    12481 tcaggtagaa caagcagcaa gcagtttgtc tgaaactgct tgctttttct ttatcgggac
    12541 ggaaccgtgc tgactttaga tgcgtttgag caatgcttga aggattgttt tcctcaaggt
    12601 ctgaatggaa aaaaaacagc ggtggcatta agcggcggct tggattccgt cgttttgctg
    12661 catctgcttg tccgcgccgg aaaaaagggc ggttttattc cggatgcatt gcatatccat
    12721 cacggcttga gtccccgtgc cgacgattgg gcagatttct gccaaaacta ttgcgatatg
    12781 ctcggggtgg ggctggaaac ggttaaggtc tgcgtggaaa aaaacggttt gggcatcgag
    12841 gcggcggcgc ggcaaaagcg ttatgccgcg tttgccgaaa aaggctttga cgttttggcg
    12901 ttggcgcacc acagggacga tcaaatcgaa acctttatgc tggcagtcgc tcgcggcggc
    12961 ggtttgcgcg ctttggcggc tatgcccacc gtccgccctt ttggggaaaa aggcatcatc
    13021 tggcggcctt tgctgccttt ttcgcgccaa gacatatggg attatgccca aaaacacggt
    13081 ttgccgaata tcgaggacga aagcaatagc gatacggctt atttgcgaaa ccgcttccgg
    13141 caccgtattt tgcccgaact ttcggcgcag attccccatt tcgggcggca tgtactgaac
    13201 aatgtccgcg ctttgcagga agatttggct ttgttggacg aaatcatcgg tcaggactgc
    13261 cgttgggtct gtgaggaagg cagtttcgat acggtgcgtt ggcaggcgtt ttccccacgc
    13321 cggaaaaccc atattttgcg gtattttttg aaggaaaacg gcatccccat gccgaatcag
    13381 aatgcccttg ccgacattgc ccgggttttg acggaggcaa aaaccggacg ttggaacttg
    13441 caaggctttg aattgcatca ttatgcaggc aggctgtttg tgttccgact ggaaaaaacg
    13501 gataaactgc ggtttttgaa agacaggcag ataagcggaa atttaaggga aatattgacg
    13561 gggcagggat ttgttttaaa acgtcatccg tttgggcttc ctgagcatct tttggagcag
    13621 gacggaattt tgaggacggt agcggtatcg gatacgttgg ctgtcggcgg catccataag
    13681 gatgtgaaaa aaatccttca ggggaaacgg gttttgcctg tcctgcgccc aatttggccg
    13741 cttgttgccg acagcggaaa ccgtccattg gcgttggcaa actgttgtgc ggatttccaa
    13801 tactcggttt cagacggcat tttgcccgtc catcctgact ttcccatttt attttgataa
    13861 tatcgcaaac agatttcggc ggcgttcaga cgggtattgt ccggttgcat atttctaaaa
    13921 ggcttgtgaa gtgaaacaca tcagttcgac caataatgaa cacatccggc acctgcaccg
    13981 cctgttgtcg caaggaaagt tcagacggca atacgcccaa accgttttgg agggcgtgca
    14041 cctgcttcag gttttcctgc aatccggcag aaaaccggtc ggggtatata ttcccgaggg
    14101 caaaatgccg tctgaagaag tccgcaggct gagggcggtt gtgccggaag gcaaggtttt
    14161 ttccgtttca gacggcatat tgaaaaaaat cagcagcttg acttgtgcgg atgatgtgct
    14221 tgcgctgatt gatattccgg atgcgggtgc tttgcctgcc ggcggcgact gcgtggtttt
    14281 ggacggcgtg caagacccgg gcaatgtcgg cacggtgttg cggagcgcgg cggcggcagg
    14341 ggtaggtacg gtcgttttgg gcaggggttc agcggatgtt tggtcgccca aggtattgcg
    14401 cgcggggatg ggcgcgcatt tcttgttgga catttattcg caggcggatt tggaaatatg
    14461 gttggcgagc tataaaggcc gtgtgtttgc caccgccttg cgagaggaaa agcaggcggt
    14521 tttgtacggc gaagatttgt gcgaaccgac agcctgggtg tttggcaacg aaggcgcggg
    14581 ggtcggtaaa gcggttttgg acagggcgga caagtgtgtc aggattccga tgcacgatgc
    14641 aaccgagtct ttaaatgtcg cgatggcggc gacaatctgc ctgtttgaac agatgcggca
    14701 gcggtcggcg tattgaggaa aagaaatgcc gtctgaaaaa atctattacg gcgtattgat
    14761 tttcttatgt atcgcttcta tgctgctgtc gccgtttttt tatgcgggtg ctttgaagcc
    14821 caagaaggcg gcattgcgga aggacgggca gtggaaactc atcttattgt ccaataccgt
    14881 ggcggcggcg gttttgtttt ggatgtggtg gaaatggttt tgacagatat tgctcaaaat
    14941 tgggttaatg gaatccgaac aaataaataa tttggtaaaa aatttgttaa atcaatggat
    15001 taaagttttt cgggaaacaa aacagctcta agcaaatagg gcgtttgtcg gtaaatacgg
    15061 aagagttgcg gcattatcgg gcatctttaa caagtagtgc cggcttggca ggcaattggt
    15121 ttttatgggc agcttgcaaa atcgcggata taaaattgcg aatcggttaa agcgtgagga
    15181 taccatgaaa aattgcaaat ttttttatga tccgacaagg gcaatctatg atagcggtgc
    15241 agattattta accagagaaa aacatagatt agtcgtaatt gcaaatagtg cttgggggct
    15301 attgcttaat ttatcttgtt attatgacga ggttttggaa aagcggaaaa taccgttcgg
    15361 caaacaggaa attgatgacg atatggacaa agtgtccgcc cttaagcgga agtttaaaga
    15421 tatttctgaa atcaaagtag gggatggttg ggaatacccg ttcaattatg agcagggaat
    15481 gaaagaatta gatgaagtgc tattgaaata cattcccttt tttgaagaag aacgataaag
    15541 gaggttgata tgcgcgtatc taaaataatt ggaagtatgt tgcttgttac agcggttcag
    15601 accgtatttt cggcaaatgt ttacgcgtgc cgccataatg gtaaaaccag ttacagccaa
    15661 actccgggaa aacattgtac caacgcgggt ttggggcggg acctggtgta cagttcggtt
    15721 agaccggcag taaaagacag ggcggaagac gcaggggtcg gcgattattc ggacacggtg
    15781 agggacgaac acgtccaaaa tccgagagag aatacgcata aggatgcttc gaataccggt
    15841 gcaaagacgc attgattgaa accgaatcag ccatgacggc gttgaataat agaatttgaa
    15901 cgattaggga actctgatga aacacatcca cattatcggt atcggcggca cgtttatggg
    15961 tgggattgcc gccattgcca aagaagcagg gtttgaagtc agcggttgcg atgcgaagat
    16021 gtatccgccg atgagcaccc agctcgaagc cttgggcata ggcgtgtatg aaggcttcga
    16081 caccgcgcag ttggacgaat ttaaagccga cgtttacgtt atcggcaatg tcgccaagcg
    16141 cgggatggat gtggttgaag cgattttgaa ccgtgggctg ccttatattt ccggcccgca
    16201 atggctggct gaaaacgtgc tgcaccatca ttgggtactc ggcgtggcgg ggacgcacgg
    16261 caaaacgacc accgcgtcta tgctcgcgtg ggttttggaa tatgccggac tcgcaccggg
    16321 cttccttatc ggcggcgtac cggaaaactt cagcgtttcc gcccgcctgc cgcaaacgcc
    16381 gcgccaagac ccgaacagcc aatcgccgtt tttcgtcatt gaagccgacg aatacgacac
    16441 cgcgtttttc gacaaacgct ccaaattcgt gcattaccgt ccgcgtaccg ccgtgttgaa
    16501 caatctggaa ttcgaccacg ccgacatctt cgccgatttg ggcgcgatac agacccagtt
    16561 ccaccacctc gtgcgtaccg tgccgtctga aggcctcatc gtctgcaacg gacggcagca
    16621 aagcctgcaa gacactttgg acaaaggctg ctggacgccg gtggaaaaat tcggcacgga
    16681 acacggctgg caggccggcg aagccaatgc cgatggctcg ttcgacgtgt tgcttgacgg
    16741 caaaaaagcc ggacacgtcg cttggagttt gatgggcgga cacaaccgca tgaacgcgct
    16801 cgccgtcatc gccgccgcgc gtcatgccgg agtcgacatt cagacggcct gcgaagcctt
    16861 gagcacgttt aaaaacgtca aacgccgcat ggaaatcaaa ggcacggcaa acggcatcac
    16921 cgtttacgac gacttcgccc accacccgac cgccatcgaa accacgattc aaggtttgcg
    16981 ccaacgcgtc ggcggcgcgc gcatcctcgc cgtcctcgaa ccgcgttcca acacgatgaa
    17041 gctgggcacg atgaagtccg ccctgcctgt aagcctcaaa gaagccgacc aagtgttctg
    17101 ctacgccggc ggcgtggact gggacgtcgc cgaagccctc gcgcctttgg gcggcaggct
    17161 gcacgtcggc aaagacttcg atgccttcgt tgccgaaatc gtgaaaaacg ccgaagtagg
    17221 cgaccatatt ttggtgatga gcaacggcgg tttcggcgga atacacggaa agctgctgga
    17281 agctttgaga tagcccgggc gatgccgtct gaaagccctt cagacggcat cgcccggctg
    17341 cgcggcacaa aggcggaaaa accgtttgcc ccgtattttc aaacgcgtta cacttgccgc
    17401 cgctgttttc agccatttga ttacccgcaa ccgccgtcat tgcgccggcg gtttgcctgt
    17461 cagcgtcatt gcgccgctgt aaatacgaaa gaacacatta tgaccgtatc ccccgtcgcc
    17521 ttgcgccgta agaccgagtg caagcctcat cccaccgcgc gctattggaa aaaatgcgat
    17581 gtcgaagccc tgttcggact tcccttcctc gacctcattt accaagccgc cgaaatccac
    17641 cgccaaaatt tcaacccgcg cgaaatccag ctttccacgc tgttgtccat caaaaccggc
    17701 ggttgtcccg aagactgcgc ctattgtccg caatcggcgc accacaatac caatctgggc
    17761 aaagagcaga tgatggatgt ggatgaaatc gtcgaaaaag ccaaaatcgc caaatcgcgc
    17821 ggcgcaagcc ggttttgtat gggcgcggcg tggcgcggcc ctaaacccaa agacgtggag
    17881 acggtttccg caatcatcaa agccgtcaag ggcttgggta tggaaacctg cggcacgttc
    17941 ggtatgctcg aagaaggtat ggcggaagac ttgaaagagg cgggcttgga ttattacaac
    18001 cacaacctcg acaccgaccc cgaccgctac aacgacatca tccacacccg ccaacacgaa
    18061 gaccgaatgg acaccttggg caaagtccgc aacgccggtt tgaaagtctg ctgcggcggc
    18121 atcgtcggga tgaacgaaac ccgcgccgaa cgtgccgggc tgattgccag tctcgccaat
    18181 ctcgacccgc agcccgaaag cgtgccgatt aaccggttgg tcaaagtgga aggcacgccg
    18241 cttgccgatg ccgaagattt ggactggacg gaatttgtcc gcaccatcgc cgtggcgcgg
    18301 attacgatgc cgcaaagtta tgtccggctg tcggcagggc gcagcaatat gccggaagca
    18361 atgcaggcga tgtgctttat ggcgggcgcg aactcgattt tttacggcga caagctgctg
    18421 accacgggca atcctgatga ggacggcgac agaatcctga tggaaaagct caacctgtat
    18481 cccttgcagt ttgaaccgga aggcgaggtc gccgaagtgg aaaaagcctc tgggattaaa
    18541 gtggattatt gacgattgaa aaatgccgtc tgaaacccgg aaaaaggctt tcagacggca
    18601 tttgtccgga cggcatttcc aatatctttt taccggcgcg tgatgctgcc gtcgggcgag
    18661 acatccagcc cgttcccctt ggggaaatcg ctgcggttca gataaataat ccgcccgata
    18721 acggttttct cggggtcgat tttggggatt ttatcgccga tttgagtgat ggggataatg
    18781 ttgccggaaa cgaaccgccc ctgtttgtcg gtgataattt tgaaaatcgg cgcaatgccg
    18841 ctgatgccgg aggtgttgat tgcgccgtag gtggcaaagt tgccgccgct gtaggagatg
    18901 aagcggtcgc ggtaaagttc gacggcgcga gtaacgtgcg acccctgccc gaatacgaca
    18961 tccgcgccgg aatcgacggc aagccgtgca aactcgacga cgttgcccct gttttcccca
    19021 tagaagattt cggtatcgaa cggcaggtgt tccgcctgtt tcccttccgc gccgccgtgg
    19081 aacatcacaa tgacgatgtc ggctttctgt ttggttttgg taatccgttt tctaactttg
    19141 gcataatcgt tcagtttgac ggcggcaagg ttgggggcga aggagacgaa gccggatctt
    19201 acgccgtttt tcttcaggat ggcggtttca aacctgtttt cgatgcccga atatttgatg
    19261 ttcaattcgt caaggttcgc cctttatgcc gtttccgcac cgcaaaccgc cggggtcaag
    19321 ccctcggcgc atcctgccgg aacggaatcc ccgtgctgcc gattgacggt tcaaaccccg
    19381 cgcccgtttc aaataccggc gatgtggacg gacaggatgc gcctgacgaa aagacagccg
    19441 ataccgtttc cattatcggc gtgggcgaca ttatgctcgg cagcaattat ccggtcgatt
    19501 acctgcccga taccaatatt ctgaaaaacg tcgaatctgc cttgcaagac gcggacatta
    19561 ccgtcggcaa cctcgaaggc acgctgtttg acgaaggcgg tacgccgaaa aaatgtgcaa
    19621 acccccaaaa tatgctatgc attccgaacg ccctccgcat acgggcaata ccttgccgac
    19681 gcgggattcg actacctcag cttcgccaac aaccacagca acgacttcgg cgcgcaaggc
    19741 atcacggcaa cggcggcgag cggcagctct tttacatact cgatcgcgct aaagccgctg
    19801 ccgataacga ggccaaaatt gcggaaaata ccgccatcgc ccagataaat ttgtccatca
    19861 tcagaccttt actgttcaga cgagacagca tttgccgcac gttttggggc ttatctttcg
    19921 atttgcgcta cgtcgcgcac cgcgcctttg tcggcggaag tcgccatcgc gccgtaagct
    19981 cttaatgcgg cggagacgta gcggtcgcgg tttttaggct tccatgcttt gctgccgcgc
    20041 gcttccattt cggcacggcg tgcggcaagc tcttcgtcgg aaatggcaag gtggatgctg
    20101 cggttgggga tgtcgatttc gacggtatcg ccttcgtgta ccaaaccgat cgcgccacct
    20161 tccgccgctt cgggcgaggc gtgtccgatg gacaaacctg atgtgccgcc ggagaagcgt
    20221 ccgtcggtta agagagcgca ggctttgccg aggcctttag atttcaggta ggaagtcgga
    20281 tacagcattt cctgcatgcc cgggccgcct ttcgggcctt cgtagcggat gatgacgatg
    20341 tcgccggcga cgatttggtt gcccaaaatg ccttctactg cgtcttcttg gctttcaaac
    20401 acgcgggcgc ggccggtgaa tttgaggatg ctctcatcca cgcctgcggt ttttaccacg
    20461 cagccgcgtt cggcgatgtt gccgaacaag accgccaaac cgccgtcttg cgagtaggcg
    20521 tgtgccacgt cgcggataca gcctttttcg cggtcgaggt cgagggtttt ccacatacgg
    20581 ttttgcgaga acgcttgggt ggtgcgtacg ccgccgggcg cggctttgaa gcgttcgatg
    20641 gcgcgggtgt tttcagggtt ggttacgtcc catttttcaa tcgcgtcttt cagggtcagc
    20701 gcgtgaatgg tgtacacgtc ggtgtgcagt ttgcccgctt tgtccaattc tttcaggatg
    20761 gcgaagatgc cgccggctcg gtgcacgtct tccatatagt agtcgtggtt gttgggcgcg
    20821 gttttgcaga tgcaaggcac aacgcggctc aggcggtcga tgtccgccat tttgaaatcg
    20881 acaccggctt cgttggcaac ggccaacagg tgcaaaatgg tattggtaga gccgcccatg
    20941 gcgatgtcca tggtcatggc gttttcaaac gcttttttgg tggcgatgct gcgcggcagt
    21001 acggtttcgt cgtcttgctc gtaatagcgt ttggtgattt cgacaatcat acggcctgct
    21061 tcgaggaaca attctttgcg gccggcgtgg gtcgccaaat acgaaccgtt gccgggcagg
    21121 gaaaggccga gtgcttcggt caggcagttc atcgagtttg ccgtaaacat acccgaacac
    21181 gagccgcagg tcgggcaggc gttttgttcg acttcctcga cttgccggtt gctgacattg
    21241 tcgtccgccg attcgatcat cgcgtcaatc aagtccaaac ggcgttcggg ttggatgttt
    21301 gccacgccga taactttgcc cgcttccatc gggccgccgg aaacgaagat ggtcgggatg
    21361 ttcaggcgca tcgcggcaat cagcatgccc ggggtgattt tgtcgcagtt ggaaatgcac
    21421 accagcgcgt cggcgcagtg ggcattgacc atatattcga tggagtcggc aatcaaatcg
    21481 cggctgggca gggaatacag cataccgctg tgtcccatag cgatgccgtc gtcgatggcg
    21541 atggtgttga attctttggc gatggcgccc gccttttcga tctcgcgggc aaccagctgg
    21601 cccatattgt gcaggtggac atgacctggc acgaattggg tgaacgagtt ggcaacggcg
    21661 ataatcggtt tgccgaagtc ggtttccatc acgccggtgg cgcgccacaa cgcgcgcgcg
    21721 cccgccatat tgcgaccgtg ggtggaggtt ttggagcggt attctggcat agtgtgtttc
    21781 cttgtgccta taccgtctga aagacagggc tgtttcagac ggtatcgggt acggtttttt
    21841 agagtgggaa aagagggtat tttataccaa gtatcggaat tttgcgggat tgaaacggcg
    21901 tgcggcaaaa aagaaaatcc ccgcaggaat gcggggacgg gttcaggcgc gggcaatcgc
    21961 gacggctttg gatgcgtccc aaaaatcaac gggtgcattt aatacgggtt tgacgatgcc
    22021 cgtccgtatg gcgaaatcgc cgaaaccttc gccgatattg cgttctgccg cccatttgcc
    22081 gatcaggtcg tccaattcgg caaggatttc gggcagggtg atgttttctt tgtaaagacg
    22141 ggggatgcgt acgccttcac ggtcgccgcc gatatggagg ttgtagcgtc cgacggcttt
    22201 gccgaccagt ccgatttccg ccaacatcgc ccgtccgcag ccgttcgggc agccggtaat
    22261 gcgggtaacg atgtagtcgt ccgacgtgcc gtgtttcgcc ataatcttat ccagctcgcc
    22321 gatgaagtcc ggcagcacgc gttcggcttc cgccattgcc agcgggcagg tcgggaagga
    22381 aacgcacgac atcgcatttt cgcgcagttt gctgacaccg tcgtgtatca gcccgtatga
    22441 gcgggcgagc tgttcgatcc gggctttgcc tccttcgggt acatttgcca cgatgaggtt
    22501 ttggttggcg gtgatgcgga agtcgccttt gtggattttg gcgatttcca acacgccggt
    22561 cagaagctgt ttcccgcctt cgtcaaccaa acgcccgctt tcgatgaaaa gggtcaaatg
    22621 ccagttgccg tctatgcctt tcacccagcc gatgcggtcg ccgcgcccgg taaatttgaa
    22681 cgggcgtatg ggttcgaacg gcatacccat acggcgttcg acttccgcgc ggaagttgtc
    22741 caagcccata ttttgaatgg tgtagcgggt gcgggcgttt ttgcggtcgc tgcggttgcc
    22801 gaagtcgcgc tgtgtggtta ccaccgcttc ggcggccttc agcgcgtgtt ccggaggcac
    22861 gaaacccagt tccagtgaaa tgttcggata ggttttggtg ttgccgtgtt ccatcgaaag
    22921 cccgccgccc gccaaaacat tgaatccggc aagctgtccg ttgccgtctg aaatggcgac
    22981 aaaatccaag tcgttgccgt agcagtccac atcatttaag ggcgggatga cgactgcggt
    23041 tttgaatttg cgcggcagat aggttttgcc caaaatcggc tcgtcttctt gaaggaagtc
    23101 gtcggaactt tgaacttttt tgccgtccac ccacacatcc agataaccgc gcgtgcgcgg
    23161 cagcaggtgt tcggaaatct ttttcgcgta ttcgtaagcc tgccggtgca gttcggactc
    23221 gatcgggttg gacgtgcaaa gcacgttgcg gttcatatcc gccgccgtgg cgatggaatc
    23281 caaacccagt ttgtgcaaga ggcggtgcat cgtctgcaac ttggctttcg gcacgccgtg
    23341 aaattggaag gtttgccggt tggtcagccg gatggagcgg taatgactgt tttcccgggc
    23401 aaatttgtcc agttctatcc attgggacgg tttgatgatc ccgcccggca gccggcagcg
    23461 caaaagcata aatttcaagg gctcgagttt tgcctcggcg cgttcggcgc ggatgtcgcg
    23521 gtcgtcctgc tcatacatac cgtggaagcg gatgagttgg aagttgtcgc ctttgaagcc
    23581 gcccgtgagc gggtctttca aatcgtccaa aatcgtgccg cgtaaaaaat tgctttcggt
    23641 tttcagacgt tcgttgtcgg atagcggttt ttcttgccac gccaaacctt ttgtcttggt
    23701 ctgtacggtc attttgtgtt cctcccgatt atatttaatc aataaacatc acgctgatag
    23761 cgtttttctt cgcgcagcat atccaaatat tcttctgcgc cctcttcgtc caaatgtcct
    23821 gccccgataa tcacatccag caaggcggct tccacgtctt ttgccatttt tgccgcatcg
    23881 ccgcacacat agatatgcgc gccttcctgc agccattgcc aaagtccttc cgcctgttcg
    23941 cggattttgt cctgcacata gattttttct tcctgatcgc gggaccaggc gaaatcgtac
    24001 ctgtgcagga aaccgtcttt ggcaaactgc tgccattcgg tttgatagag aaaatcacgg
    24061 gcaaaatgcg gattgccgaa aatcagccag tttttgcctt ccgcattttc tgcggcacgt
    24121 tgttggacga aggcgcggaa cggtgcgacg ccggtgcccg agccgatcat cacaatcggc
    24181 ttgcggctgt cttcgggcag cctgaagccg tcgttgcgtt ccacaaacac gcgcaccgtg
    24241 ccgtcctctt ccagccggtc ggcaaggaaa cccgatgcgc cgcccgccct ggcgcgccct
    24301 tcgtgttcga aacgcaccgc gccgacggtc aggtgcactt cgtcccccac ttccgcctgc
    24361 gacgaggaaa tcgaatacag gcgcggcgca agcgggcgca gtaggccggc gaattgttcc
    24421 gccgtcagtt ttgccgggaa gcggtgcagc acatcggcaa tcggcgtgct ttgcacaaag
    24481 ccttgcaaaa cggcgttgtc ggcagcaata cggtcgagtt cgtcatcatc ggcgaacggg
    24541 gcatagcctt tgacaaaggc gggggtgttt tgcgtgagtt caaaatggga taacagtgcg
    24601 gaggcaaccg gcagggtttt tccgcccgcc tgtatttccg ttgccggatc gatgccgagc
    24661 aggtctagga tttccctgac cagtgccgga tcgttgtcaa accaaacgcc gagcgcgtcg
    24721 cccgggaggt agtgcaaatc cgaaccgctc aaatcgattt cgatgtggcg cacgtcttta
    24781 tcggattggc gggcggtgat tttctgattg gccagcaggg cggcgggaaa ggggtctgcc
    24841 ttgcagtacc tgccatccgg tgccgtctga aggccggcgg ggggcgttgt ctgcggcgcg
    24901 ggcgttgccc ggttttttgc ggcttcttct tttaagcgcg cgacaatgcg gcctgtccat
    24961 ccgtctgcgg cggcggcaaa gtccaaatcc gcatcaacgc gttcgagcag gcgttttgcg
    25021 cccaattctt caaaacgccg gtcgaaatct ttgcccgccc ggcagaaatt cggataggaa
    25081 ctgtcgccca aacccagtac ggcaaattgg agtttgtcca atttcggggc ttttttgccg
    25141 ttcagcagtt tgtgcagcac gacggcttct tccggcggtt cgccttcgcc ctgggtggac
    25201 gtaaccagca gcaggcggcg ttcgtcggcg atgtttttcg ccttatagtc tttcagttcg
    25261 gcgcgcctga cttggatgcc ggcggcttcc aggctgtccg acgctttgtc ggcaacggat
    25321 ttcgcattgc cggtttgcga ggcggaaagg acggttacgg aaaaaggttc tgccgccggc
    25381 aatgccgtct gaagcgcggg cagccctgcg gatgccccgt ttcctgcttt tgcccaagcg
    25441 tagccggaca gccacgccca ttgtgccgcg tccagccccg acaggagctg cgtgatttcg
    25501 ggcggcagag gcggtaatgg cggatttgtg ttctgcatat cgtgttcact cataaaatca
    25561 tacctgccgc aacagtgccg tatgtcgctt cgtctatcag gataaacgaa ccggcggcgg
    25621 tgttttccgc ataaggcgtt gccgtaacgg gtttttgaag gttgatgcgg actttggcga
    25681 tgtcgttcat cttcaaggat tccgcgccgg cctcttgttc cagcgtgcgg acatctaaaa
    25741 cgctttcaat ttccccgact tttgccggca cggtttgcgt gccgtgcttg agcaggtatt
    25801 tgcgcgcggt gttgagcgga cgttcgtcaa accagcaaag cgtggcttcc agatgttttt
    25861 gcggggcgag cggggaattt ttatcgacaa aaaggtcgcc gcgcgaaaca tcgatgtcgc
    25921 ggtccagccg gagggttgcc gcctcgccgg caaaagcctg cgccacttcc cctttcggcg
    25981 tgatgatttc ggacacttcg gcggtcagcc cgttcggttc gatgcggacg gtttgcccga
    26041 cggcgaccga accgcgttcg atgcgcccct gatagcctcg gaaatcatcg gccttgtcgg
    26101 catcttggcg gacgaccagt tggacgggga aataaaaatc ggcagcggaa cgaccggttt
    26161 cgtccgcccc cggcaggttt tccaagatga ccagcagcgg ttcgccccga taccacggca
    26221 tattgccgcc ggggtaaacg aggttgtcgc ccaagagtgc ggacaccggt acgaaatgca
    26281 cgtctttcaa accgagctgt tcggcaagtc ggcggtatgc ctccacaatg gcgttgaatt
    26341 tgtcttcgct gtaatccagc aggtccattt tgttgaccgc caccacaata tgcgggcagt
    26401 tgagttggcg gaggatggcg gaatggcgtt tggtctgcgg cagaagctgc aagggctgcg
    26461 cgccgaaatc cagttgggat gcgtcaacca gcacgactgc cgccgaagcg gtgcttgcgc
    26521 ccgtaaccat attgcgcgtg tattgttcgt gccccggcgt gtcggcgatg atgaatttcc
    26581 gtttcgccgt ggaaaaatag cggtatgcca catcgatcgt aatgccctgt tcgcgttcgg
    26641 cttccagtcc gtcggtcagg atggagaagt ctatggtttc tttcaaacct ttgcttttgc
    26701 cggattccaa ggttttgatt tggtcggaca gcagggcttt gctgtcgtag agcagtcgtc
    26761 cgatcagggt gcttttgccg tcatcgacgc tgccggcggt aatgaagcgg agcggggcct
    26821 ggtgttgtgc cgtcatattt tcttcctcat atctgcttaa agggtttttg aaatttagaa
    26881 atagccttct tttttgcgtt tttccattgc cgcctcgctt gcctgatcgt ctagccgggt
    26941 cgcgctgcgt tcggaaatgt cggcaaccgc cgtttccctg ataatctccg ccggcgtgga
    27001 cgcggtgctt tctaccgggc aggtgcagct gatgtcgccg acggtgcgga agcggacatc
    27061 aaggatttcg gaggtttcag acggcatttt cggggtgagc ggcgttacag ggaccagcag
    27121 ccccctgcgt ctgaccactt cgcgcctgtg gctgtaataa atcggcggca gctcgaggtt
    27181 ttcgcgggcg atgtattgcc agatgtcgag ttccgtccag ttggaaatcg ggaagacgcg
    27241 catattttcg cctttgtgca gcctggtgtt gtacagcgac cacagctcgg ggcgttgcgc
    27301 cttcggatcc cattgtccga actcgtcgcg gaacgagaaa atccgttctt tggcgcgggc
    27361 tttttcttcg tcgcgccgcg cgccgcccat cagcgcgtca aagccgtttg cctcgatggt
    27421 ttccaacaag gtaaccgcct gtgccgcatt gcgcgaatcg gtttctttgc gtaagaccac
    27481 cgtgccttta gcgatggagt cttccacgcg ccccactatc aggcgggcat tgagttttgc
    27541 cgcctgcgcg tcgcggaagg caatcacttc ggggtagttg tgccccgtgt cgatatgcac
    27601 cagcgggaag ggcagtttta ccggcctgct gcccagccgg aaggctttgc aggcgagggc
    27661 gagcaggacc acggaatctt tgccgccgga aaagagcagg gcggggtttt cgcattctgc
    27721 cgccacttcg cggataatgt ggatggattc ggattccaac cagtcgagtt ggacgttgtt
    27781 cggttcggtt ttcgtcatac gatattcctt atttcttctg tttgatcctt atgaattatt
    27841 tgtgcagccc gcattctttg ctgtttttat cttcccacca ccaccgcccg gcgcggatgt
    27901 cttcgccggc cttgacgggg cgggtgcagg ggtcgcagcc tatactggga aagccttgcc
    27961 ggtacaaatc gttgtaaggc acattgttgg caaggatgta tgcccacacg tcgtgttccg
    28021 accagtcgaa aatcgggttg tatttatcga tgccgcgccc ggcatcatat tcggcaaacg
    28081 gcagttccgt gcgtgtggcg gactgttcgc ggcgttgccc ggtaagccaa gcatccgccc
    28141 cggcaatggc gcggtcgagc ggttcggttt ttcggatgcg gcagcattcg cggcgcgctt
    28201 caacgctgtc gtaaaaggca aacctgcctt tgctttccac ataatgcagg gcatcttccc
    28261 gtatcggttg gaaacgcttg atttgcatat gcggataaac acgttcaatc ctatccaaca
    28321 ggtttaaagt ttccgcgtgc agcagccccg tatccaaagt aaaaataccg atattgaggt
    28381 tttctccggc gataaggtcg gtaattacca tatcttccgc agcaaggctg ctggcaaacc
    28441 gtgcatcccg gtggctgccg gcaatccggt gcaggcgttg tttgagggtt tccgtttttt
    28501 ccgccaaagc cgtttcactg ccattttcaa tcggcggaat tttccataaa gcaggtttga
    28561 aaattttcat tttgtccgcc ttgctttatg cggcttgttg ttcttgtccg gtgcccagtc
    28621 cgtccaatgc aaaatacgcg tgttcggccg gattgctttc ttttttggcg ttttcgccga
    28681 accaagccag tttttcgtgc agccccacca cttcgcctat gacaatcaat gccggattcg
    28741 gcgcggtttc ggcgagttcg gaaaggttgg cgagcgtgcc ggttgcggtt ttttgagccg
    28801 gcagcgtgcc ttggctgata acggctgccg gcgtgtcggg cgagcgtccg tgctgttgca
    28861 gccgttcggc aatcagggcg gctttgagcg cacccatata aatcaccaag gtctggcggc
    28921 tgcgggcgag ggtctgccat tcgatgtcgg gcgcatccgc cttgcggtgg ccggttacga
    28981 aaaccgcact ttgggcataa tcgcggtgcg tgagcgggat gccggcatag gcggtcgcgc
    29041 cgacggcggc ggtaatgccg gggacgaccg aaaacgggat ctgatggcgt gccaaggttt
    29101 ccaattcttc gccgccgcgt ccgaacacga aagggtctcc gccttttaaa cgcaccacgc
    29161 gcctgccttc gcgggccagc ctgaccataa gcgcattggt gtcctcttgc ggggtgcggc
    29221 cgccccgggc gcgcttgccg acaaaaatcc gttccgcatc gcggcggacg agggacagta
    29281 tgccgtctga aaccagcgcg tcgtaaagca ccacgtctgc ctgctggatt tcctgcagcc
    29341 ctttgagcgt cagcagcccc gcatcgccgg gacccgcacc gaccaaagac acaaaaccgc
    29401 cttgataatt ttgttccaac tgctttgcca attcccgttc ggcaagggtg ttttgccggt
    29461 ttttgacgag ggcggcgaaa cgtccgttaa actgcttttc ccaaaagcgg cggcgttcgg
    29521 taacggattt cagtttgccc ttgacggcat cgcgccacct tcctgaaatt tccgccatat
    29581 cgcccaaaga cggcggcagc agggcttcca gcctttcacg cagcagtcgg gcgaggacgg
    29641 gcgcgctgcc ggagctggaa acggcaatct gaaccgggtt gcggtcgata accgacggga
    29701 agatgaagct gcaatggtcg cggtcgtcca ccacgttgac cggcttttgg cagctttcgg
    29761 caagatggaa aacgcgccgg ttgagggctt ggtcgctgct tgccgcaatg atgaggaaaa
    29821 ccgtgcggat gtgttcggca cgaaattctt cggcaagcca caggattttg ttttccgccg
    29881 ccaacgcgga gagttcggca ttcaggtgtt ttgcggcaac cctgacctct gcgcccgcct
    29941 tcagcagcag gctgattttg cgtgcggcga ccgcgccgcc gcctacgacc aatacggggc
    30001 ggccggcgag gttggcgaaa atagggaaat aattcactgg ctgactcctt tgctgtttgc
    30061 ccgcaccttg tttccgatac ggtgcgtcgc ggcatttttg tcggaatgcg ggtcatttta
    30121 gacaaaagga ttttcccggt taaataataa aaaggcattt gttagaagct gaaagctata
    30181 tgagggcggc tgcggatgcg gcggttttcc
//
LOCUS       DAWFJW010000012        30129 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFJW010000012 DAWFJW010000000
VERSION     DAWFJW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.012"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..676
                     /locus_tag="WGR19_000441"
     CDS             <1..676
                     /locus_tag="WGR19_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0798034.1"
                     /translation="AHIRAGDRVLSKDEASGETGYKPVTARYGNPYRETVYIKVSDGI
                     GKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITFKQQPLKAYNLT
                     VADWHTYFVKGDRAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRTYSGKGTRQRSQ
                     ISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNNYNRIESPGKKY
                     RKQDGD"
     gene            679..1002
                     /locus_tag="WGR19_000442"
     CDS             679..1002
                     /locus_tag="WGR19_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0798035.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQNEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1061..1288
                     /locus_tag="WGR19_000443"
     CDS             1061..1288
                     /locus_tag="WGR19_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798036.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1415..1957
                     /locus_tag="WGR19_000444"
     CDS             1415..1957
                     /locus_tag="WGR19_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0798037.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LELSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            2122..2799
                     /locus_tag="WGR19_000445"
     CDS             2122..2799
                     /locus_tag="WGR19_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0798038.1"
                     /translation="MAHIQTGEYVFAKDEASGKTGYKPVTARYGNPYQETVYIEVSDG
                     IGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVVKPTPLKAYNL
                     TVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSVKSRAPTNGQA
                     ALDNSVQVKSTSPRRVGVDKANNEIVVLDKTQTFNNGFAEYHGHVRSWQDLHTDQKNA
                     LKKAGLD"
     gene            2825..3154
                     /locus_tag="WGR19_000446"
     CDS             2825..3154
                     /locus_tag="WGR19_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798039.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            3571..3972
                     /locus_tag="WGR19_000447"
     CDS             3571..3972
                     /locus_tag="WGR19_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798040.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            4154..4543
                     /locus_tag="WGR19_000448"
     CDS             4154..4543
                     /locus_tag="WGR19_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798041.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(4584..6041)
                     /locus_tag="WGR19_000449"
     CDS             complement(4584..6041)
                     /locus_tag="WGR19_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0798042.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILSAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(6056..6886)
                     /locus_tag="WGR19_000450"
     CDS             complement(6056..6886)
                     /locus_tag="WGR19_000450"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0798043.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(6964..7353)
                     /locus_tag="WGR19_000451"
     CDS             complement(6964..7353)
                     /locus_tag="WGR19_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0798044.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNS"
     gene            complement(7489..8013)
                     /gene="nspA"
                     /locus_tag="WGR19_000452"
     CDS             complement(7489..8013)
                     /gene="nspA"
                     /locus_tag="WGR19_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0798045.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     AHLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            complement(8184..8378)
                     /locus_tag="WGR19_000453"
     CDS             complement(8184..8378)
                     /locus_tag="WGR19_000453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798046.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            complement(8598..9773)
                     /locus_tag="WGR19_000454"
     CDS             complement(8598..9773)
                     /locus_tag="WGR19_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0798047.1"
                     /translation="MHTISFPNHTRLIALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     ATALKLFDKVNIDLMYALPNQTVQTASDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHSALENGGFIHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQCFL"
     gene            complement(9830..12355)
                     /gene="ligA"
                     /locus_tag="WGR19_000455"
     CDS             complement(9830..12355)
                     /gene="ligA"
                     /locus_tag="WGR19_000455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0798048.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYCLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPDFKISENKAQALWELAGKSIEGLQTDKALPTDWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGILPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(12503..13789)
                     /locus_tag="WGR19_000456"
     CDS             complement(12503..13789)
                     /locus_tag="WGR19_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0798049.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSASKPADAPAKPIPVPQTPTKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQEEMLKVGIQPGGKTALRLFS"
     gene            complement(13983..14555)
                     /gene="ampD"
                     /locus_tag="WGR19_000457"
     CDS             complement(13983..14555)
                     /gene="ampD"
                     /locus_tag="WGR19_000457"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0798050.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFKYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            14639..15634
                     /gene="mltG"
                     /locus_tag="WGR19_000458"
     CDS             14639..15634
                     /gene="mltG"
                     /locus_tag="WGR19_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0798051.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            15694..16314
                     /locus_tag="WGR19_000459"
     CDS             15694..16314
                     /locus_tag="WGR19_000459"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0798052.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            16536..17816
                     /locus_tag="WGR19_000460"
     CDS             16536..17816
                     /locus_tag="WGR19_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0798053.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            17898..18168
                     /locus_tag="WGR19_000461"
                     /pseudo
     CDS             17898..18168
                     /locus_tag="WGR19_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18208..19242
                     /locus_tag="WGR19_000462"
     CDS             18208..19242
                     /locus_tag="WGR19_000462"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0798054.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEVGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            19448..20029
                     /locus_tag="WGR19_000463"
     CDS             19448..20029
                     /locus_tag="WGR19_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0798055.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPAGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGKNPDAGCKQAGQLGKRHQK"
     gene            20098..20280
                     /locus_tag="WGR19_000464"
     CDS             20098..20280
                     /locus_tag="WGR19_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0798056.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALGEEELKV"
     gene            20277..21038
                     /gene="kdsB"
                     /locus_tag="WGR19_000465"
     CDS             20277..21038
                     /gene="kdsB"
                     /locus_tag="WGR19_000465"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0798057.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGIEAVMTSNRHESGTTRLAEASAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            21061..21378
                     /locus_tag="WGR19_000466"
                     /pseudo
     CDS             21061..21378
                     /locus_tag="WGR19_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            21483..21680
                     /locus_tag="WGR19_000467"
     CDS             21483..21680
                     /locus_tag="WGR19_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798058.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQGIKQDAQKHFA"
     gene            21837..21929
                     /locus_tag="WGR19_000468"
     tRNA            21837..21929
                     /locus_tag="WGR19_000468"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21871..21873,aa:Ser,seq:gct)
     gene            22355..23140
                     /locus_tag="WGR19_000469"
     CDS             22355..23140
                     /locus_tag="WGR19_000469"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0798059.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            23177..24049
                     /locus_tag="WGR19_000470"
     CDS             23177..24049
                     /locus_tag="WGR19_000470"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0798060.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPIDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(24109..24720)
                     /locus_tag="WGR19_000471"
     CDS             complement(24109..24720)
                     /locus_tag="WGR19_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0798061.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            24829..25053
                     /locus_tag="WGR19_000472"
     CDS             24829..25053
                     /locus_tag="WGR19_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0798062.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            25054..25200
                     /locus_tag="WGR19_000473"
     CDS             25054..25200
                     /locus_tag="WGR19_000473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798063.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            25416..26450
                     /gene="pyrC"
                     /locus_tag="WGR19_000474"
     CDS             25416..26450
                     /gene="pyrC"
                     /locus_tag="WGR19_000474"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0798064.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(26817..27242)
                     /gene="nusB"
                     /locus_tag="WGR19_000475"
     CDS             complement(26817..27242)
                     /gene="nusB"
                     /locus_tag="WGR19_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0798065.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAIPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(27320..27796)
                     /locus_tag="WGR19_000476"
     CDS             complement(27320..27796)
                     /locus_tag="WGR19_000476"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0798066.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRILQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(27844..28167)
                     /locus_tag="WGR19_000477"
     CDS             complement(27844..28167)
                     /locus_tag="WGR19_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0798067.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            28334..29053
                     /gene="rnc"
                     /locus_tag="WGR19_000478"
     CDS             28334..29053
                     /gene="rnc"
                     /locus_tag="WGR19_000478"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0798068.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            29100..30023
                     /gene="era"
                     /locus_tag="WGR19_000479"
     CDS             29100..30023
                     /gene="era"
                     /locus_tag="WGR19_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0798069.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         7133 a         7023 c         8663 g         7310 t
ORIGIN      
        1 tgcccatatc cgagccggag accgcgtcct ttccaaggac gaggcaagcg gagaaacggg
       61 atacaaaccc gttaccgccc gatacggtaa tccgtatcgg gaaaccgttt acattaaagt
      121 ttcagacggc atcggtaaaa tacagaccct cgtttccaat aaaatccacc cgttttacag
      181 tcaaggaaaa tggatacagg caggtcgtct gaaaaaaggc gacaccctgc tttccgaaag
      241 cggcgcaaaa cagacggttc aaaacattac cttcaaacag cagccgctca aagcctacaa
      301 tctgaccgtt gccgactggc atacttactt cgtcaagggt gatagggcgg aaacggaagg
      361 ggtttgggtt cataatgatt gtccgtatgg agaaaaatat aggacagaag ttggttctta
      421 tactaatact catgaaagtg gtcgaactta ttctggaaag ggcacaagac aacgttccca
      481 aatctctgga gcaagagaag ctagggttaa taatgaccct catatagcta cagattttac
      541 tcctgccaaa aataatcgag aggcattcaa agatgaatct agaagaattg acgctcacgg
      601 tggttccaaa agtaccaata attacaatcg aattgaaagc ccggggaaaa aataccgaaa
      661 acaagatgga gattaaaaat gattgttgac gaaaaatcta cttcagatgt actgaaacaa
      721 tatcaagaac tagggtttga tttgacaaaa cctatgataa tagagttttt tataggaggt
      781 tctcaaaaga atctgcaatc aatagaaagc cgactcattt cttatcggca agattttcaa
      841 atcagtattg agcaaaatga attcggagaa atgtggactt gctattgttc cgtcaatatc
      901 gtcccttccc ttgaaaacat tttagctatt gaaactacgt tatttggtat tgcacaaaaa
      961 aatgattgta attatgaagg ttttggttcg tatgggaact aacaaaaagt gtaggttggg
     1021 ttgaaaaaac catattccca ttaaagatta gggtgtgtct atggacataa aattcaatac
     1081 tttgggggtt atcctgaatg gggtcaatcc tgaagaaaaa ttcataaaaa ttattgatga
     1141 tcaggaaaat accgggggat ttctgatact gttatcttca aatgacaaat tctcatcatt
     1201 tgacagttac gatgactggg tcgaaaactt agaaatatta aaagaatacc tgcaagaatc
     1261 tcattggata ataaagtggg tcggatagtc ggcaaacgta gtccgtctga aacgctttta
     1321 cttttcagac ggactttcta tcaatgggaa caaggcgttt tgtctcaatc tggtctatga
     1381 ttattcgata cagtgatggg aagctaagaa aaatatgatt aatcatattg aaaaatatat
     1441 aggcactatt tctcatggct cgaaaagcga ttcgggaagt cagtacaaat taaatatcgc
     1501 agctattccc tcttccccta acagggattt aaaaacttat attactttag agttaagcaa
     1561 acatgatctg aattataaaa gcagatttga aatacttttt gtatgttctt taaaatatga
     1621 tgaaaatcag attttcccat ttttaagatg gctggcagaa accatcattg agaataaaaa
     1681 aatccttctt cgaggacagg ttatttattt gcctagaagc attgttgatt caacaaaaat
     1741 ggatgcactg tatgtttccg ccccatttta tttcgacgat gattttcagg tctgttatgg
     1801 cgaacactac aatatcgttt tccctttgct tgtccccttg tataaacagg aagccgaatt
     1861 ggtggaaaaa aagggttgga atgcttttga gcagttcttg ctggataatg aagttgacaa
     1921 cctttcggat atgaatcgga aaccgtttgt ttggtaaggg ctttcttaaa tttgattaaa
     1981 ggtcgtctga aaccgtgttt aactttcaga caacctttcc ttcatttgaa acaggatatt
     2041 gggaactaag ttcttcaaaa atcctgtctc cgtcccttat acgtcggtgc tttagtcaaa
     2101 acggcagacg gctacaaagc catcgcacat atccaaactg gggagtacgt ctttgccaag
     2161 gacgaagcaa gcggaaaaac gggatacaaa cccgttaccg cccgatacgg caatccgtat
     2221 caagaaaccg tttacattga agtttcagac ggcatcggca acaaccaaac cctgatttcc
     2281 aaccgcatcc atccgtttta ttcgggcggc aaatggatta aagcggaaga tttgaaagcg
     2341 ggaagcaggc tgttatccga aagtggcaaa acccaaaccg tccgcaacat cgttgtcaaa
     2401 ccaacaccgc tcaaagccta caatctgacc gtcgccgact ggcataccta cttcgtcaag
     2461 ggcagtcagg cggaaacgga aggggtttgg gttcataatg attgtccgta tgataaaggc
     2521 aaccaacgat ataaagacgc ttcttatcat ggcaaaaatg ataattctgt gaaaagtaga
     2581 gcaccaacaa acggacaagc agctcttgat aattccgttc aagttaaatc aacttctcct
     2641 cgaagagttg gggttgataa agccaataat gaaatcgttg tattagacaa aactcaaact
     2701 tttaataacg gttttgcgga atatcacggg catgtcagaa gttggcaaga tttgcatacc
     2761 gatcagaaaa atgctttaaa aaaagcagga ttggattagt taattcaaaa ggaaaaatta
     2821 aaaaatgact gataaaagta aaacagaaaa gttgatttct tctgatgata aacaaagtgt
     2881 tatagatggc attcttgata tggtatttaa ttccaaagca tatgaagtac cgtggatttc
     2941 tgagaaattg atggaattat cgaaaaataa agacttggat attgccggat tatcgctaac
     3001 ctgtttcgga catctcgcca ggctacattc aaatatcggt gattacgata aagttatttc
     3061 tttactacat tcaaagcaag atgatccaga gcttcaaggt agggctgaag atgcgttaga
     3121 agatatttct ttatttttat ctgaaaatca ttaggaaccg taggtcgggt tgaaaaccca
     3181 acaatcaaaa tgccgtctga aaccgtgttt aattttcaga cggcatttcc ttcgtttgaa
     3241 ataggatatt gagaactgag ttcttcaaaa atcctacact tgctccttcc acggcagcac
     3301 cttggtcaaa acggcagacg gctgaaaagc aaacaccgtc cgtcgtgttg ccgtttgcgg
     3361 atgagtacgg gtcaacccca atgccgccga aaccgtcgaa gccgccttca acattgccgc
     3421 cgccaaagcc gcaaagttgg caaaaacggt aaaaccgggg agataaaagc cgatggcagg
     3481 aaagtaaatg tgaggataga cagtacggag gcagacctgc tttatccggc agggcaataa
     3541 gaaaacaaaa attagatatg gaaaacgatt gtgaagatta aaccattaca attttctaac
     3601 aataatcaca gattttatgt ggacaaaatt tgtgttaccg aacaagatgt gaacattttg
     3661 gcatccgacc gaaattatga attaaatatt gaaatattta ttgacaatat aattcatttt
     3721 caaataacag atgaatctta taaagtaaaa ttttcagaat atttatttga aaataaagaa
     3781 aaaaatgatt gggatagaaa tcctgctata aattattttt tcgagataat agatgacagt
     3841 tatatggact ggttgaaaga agaaagtttt gatttttttg aaaagaaata ttataaggct
     3901 tatattttct tttttagcga ttctgtaata gaagttatca gctcgacaga acctgtattt
     3961 tattcaaaat aacaaattat caaacaaagc tctgattaaa aacccaacaa tcaaaatacc
     4021 gtctgaagcg gtattcggct ttcagacggc atttttgata caaagcaggt aaccaaaaga
     4081 gcgtttgacg gcaaaggaga atctaaaata ccggatgtat cggttgggaa gcaatggata
     4141 aaggtaaata attatgtgga aaattagtaa agaaaattgt gaagatttag gatttgcaat
     4201 agtctgtatg ttctatgatg ctattaatct ttctgaattt aaattatggt tggatatagt
     4261 tgtcagagat actcctattg atacaattcc attgtatatt tttgatttga ttgattttga
     4321 taagagtata ggggaaattt atgatgtaat tggagtcgtt aattatggtt acatttcaaa
     4381 tgatcaaaaa aatgcattaa cgggcattgc cttcttaagg gggatagatg tctatgatcc
     4441 gcctatttca aaagaaaaag cattaaaagc cttagagaaa tatcctgaaa tttatcagag
     4501 gtttcagcat ttctttcctt ttgtagagct tccgcttctt taaaggacaa gatgccgtct
     4561 gaaaagtttt cagacggcat tttttatttc ttccagtagg cgggggtgaa gaggatgaag
     4621 acggtgaaga tttccagcct gcccaagagc atggcggtaa cgcagatcca tttctgcatc
     4681 acgtccaaac cggcgtaatt gccggcgggc ccgacttcgc ccaggccggg gccggcgttg
     4741 gtgatgcagg cgatgacggc ggtgaaggcg gtggtaaatt ccataccgct cgccatcagc
     4801 aggaagctga agaggacgac ggtcataaag tagatgaaga tgaaggacat aacggtcagc
     4861 gcgaggcggt cgggtatggc cttgccgctg attttgacgg tgcggacggc tttggggtgc
     4921 agcagcacca tcatttcgcg caggctgaat ttgaacagga cgagggcgcg tatggttttg
     4981 atgccgccgc cggtcgagcc ggagttggcg aggatgttgg cgaggaaaaa catccacagg
     5041 gaaatcagga gcggccattg tgcgaagtcg gtgttggcca gcccgtttgc caatccgatg
     5101 gagacgaagt tgaaggcggt gtagcgcagg gattcggtaa aaccggcgta atagccggtg
     5161 tgccacaggt acagggcggc ggaaaggatg ctgccggaga gcagcagcag catcgtccgg
     5221 cattcttcgt ctttccaata ggttttgagg ctgcggctgt tgagggcggc gaaatggttg
     5281 gcaaaattga tgccgccgac aatggtgaaa acgatgatga ccgcttcgat gaggggggag
     5341 ttgtaataag ctatgctggc atcgtgggtg gaaaacccgc ccagcgagag ggtcgccatc
     5401 gcgtgacaga cggcatcgaa ccagcccatc ccggcaaaat gcaggcaggc tgccgcgagg
     5461 atggtgatca gggtgtagcc gaaccaaagt tttttcgcca cttgggaaat gcgcggcgac
     5521 attttgcttt ctttgtcaat gccggggatt tcggctttga ataactgcgt gccgcctacg
     5581 ccgagcatag gcaggatggc gacggcaagg acgatgatgc ccatcccgcc cagccagttg
     5641 agcatatgcc gccaaaagtt gacggagggg gcgagcccgt cgacgtgggg gatgacggtc
     5701 gcgccggtgg tggtcagtcc cgacatcgat tcaaaaaatg cgtcggtaaa gcccatattc
     5761 gggaaataca ggtacatcgg catcgccgcc atagcggcaa acgccagcca caacatcagg
     5821 acgagggtga agccgtcgcg cgggcgcagt tcgcgcctga accggagggt ggcgagccgg
     5881 acgatgcacg agccggaaag ggtaacggtc gcggtggtgg cgaaggcggt gtacgcgccg
     5941 tccgaaaagg cgtaggagag ggcggcgggt atcagcagga taaaggaaaa cagcataccc
     6001 agtcgggaga ggacgtgggc gatgggcagg attttgtgca tagtggggcg gtccgttatt
     6061 ttgcgaagct tttccagtct atgccgccgg cggcttggac ttgggtaatt tcttccgttt
     6121 cgaggttgac ggcggtcagc tgtccgcccc acagcgcgcc ggtgtccagc gagatgacgt
     6181 tgtcggcatt cgtgtagccc agcgaggacc agtgtccgaa gatgatggtg cggtcgaggt
     6241 tttgccggtc gggggctttg aaccacgggc gcaggtaagg cggcattttt ttcactgtgg
     6301 atttgtagtc gaaatccagt tcgtttttaa aggtcagggc gcgcatccgc gtgaaggcgt
     6361 tgacgatgaa gcgcaggcgg gcatagcctt ccaaaccttc gtcccacgcg gccggtttgt
     6421 tgccgtacat tttggagaag aatttgacgt attttttgcc gcgcagttcg gcttcggctt
     6481 ctctagcgag cgattcggct ttggcgatgc gccattgcgg caggatgccg gcgtgtacca
     6541 tcacgcggct gccctcgcgt atcaacagcg gttgcgcacg cagccagtcg agcatttttt
     6601 ttccgtcggg gtgtttgagt atgggttcga ttgtgtcgct gcgtttgggc gcgccttcgc
     6661 cgcagccgac ggcgagcagg tgcaggtcgt ggttgccgag gacgatttgc acgctgtttt
     6721 cgtgccggat gcagaattgc agcgtttcga gggatttcgg gccgcggttg acgatgtcgc
     6781 ccgtcagcca gagggtgtcc gtgccgtggt tgaaaccgat tttgccgagc agcgcggtca
     6841 gttcgtcgaa acagccttgt atgtcgccga ttgcgtaatg tgccattgca gatgttgtga
     6901 agtgggaaag tgttgcggtt cggacggcat ggttttgaaa tatcatgcag tccgaacgtg
     6961 gaattatgag ttcaaaacga ggacggcttc ggcttcgacc tgcacgcctt tgggcagcga
     7021 ggcaacgccg acggcggcgc gggcggggaa gggctcggcg ataaattccg ccatgacttc
     7081 gttgaagacg gcaaaattgc ccaagtcggt caggtaggcg ttgagtttga cgatgtcggc
     7141 cagcgtgccg cctgccgctt cggcgacggc ttgcaggttt tggaacactt ggcgcgcttc
     7201 ggcgcggaaa tcgccgttgc cgacgacggt catcgtggcg ggatcgaggg ggatttgacc
     7261 gctcatgtaa acggtgtcgc ctgctcggac ggcttggctg tacgcgccga tggcggcggg
     7321 ggctttgtcg gtgtggatga tggttttgga catttcggat tcctcaaaaa atagggcggc
     7381 agaagccgca gcattcggga ttatcgtaca aaaccgccgg cttgtgtagt tgcggtggca
     7441 gaaaacaaaa ccgccgaagg ctcggcggtt tgcagaataa ggcgcatatc agaatttgac
     7501 gcgcacaccg gcggacagtt cgccggaacg gacgtttttg acagtgttga ctttgccgat
     7561 gtagttgtag cggtagccgg catccaaatc gacattcggg gtaacggcat agcttacgcc
     7621 cgtcaatacg ccgaggccga tggaggtttg gctgaagctg tcgctgccgc ccaagtcgac
     7681 ggaggcgcgg ttgaggctca agtgcgcgcc gagatacggt ttgacgggcg attgggtgtc
     7741 gaagtcgtaa atggcggacg cgccgatgct gtaaagtttg aaatcggtgg atggggcttt
     7801 atagtttttg tagcgcgtgt aatcgacggc gaagcggagg tcgttgatgc ggtagcctgc
     7861 ggagatgcgc gggctgaagc ctttggcaga acctaaagag cttgaggctt ttgcgtgtgc
     7921 ggcatcggct tggacgtaaa agccggatgc gccttccgcc agtgcggcgg ccgggagagc
     7981 gagggcaatc agtgtggcaa gtgctttttt catattttgg ttcctttatg gtcagttaga
     8041 aaaattgtta agaatccgtt aaagaatcct gctgtattat acttaaattt tctttttgca
     8101 tcgtaatatt ttcaatactt caagatacgt agcggtatcc ggctgctttg ccgacggcaa
     8161 agccgttaac ccgcgcgttg cctttaaatg gtggcggcgg catcacgcgg cggatgggtg
     8221 aaacttgcaa acggtttgga aaaaacagcg gtatctgtcg gattgttgca ggtgcaggca
     8281 tacggttttg tgtgcgtctg tgccttaagc gtcggacatt tccggcggcg gctgtgccgt
     8341 ctgaaacgcc cggcggggga tgcggctgcg ttttccatcg ataagcatat tttccggacg
     8401 cgttcggggc gggttttccc gggcggccgc cgatttgttt gcgcttatat agtggattaa
     8461 caaaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacgga accgattcac
     8521 ttggtgcttc agcaccttag agaatcgttc tctttgagct aaggcgaggc aacgccgtac
     8581 tggtttttgt taatccacta taaaaaacat tgcagcaaat cgtttaaaaa caagcgtcct
     8641 ttttcggtcg ggcggaatac ggcggggtcg gtttccagca ggcctttttg ccttgctgtt
     8701 tcgatttgcg ccatgatttt ggcactcggt acgcccgtgc gttcctgcaa catcgcggcg
     8761 ggtacgccgt cggtcaggcg cagggcgttc atcatgaact caaacggcaa atcttcggcg
     8821 gcgacggttt tgcgttcgac ggcttcgctc ggtcggtttt gcattaaggc gaggtagtcg
     8881 ttggggtggc ggcggcggac ggtgcgttcg atgcggtcgg gataggagat tttgccgtgc
     8941 gcgcccgcgc cgatgcccaa ataatcgccg aactgccagt agttcaaatt gtggcggcac
     9001 tgcatggcgg gttttgcaaa agccgatgtt tcgtagtgga taaagccgcc gttttctaat
     9061 gcactgtgta ccgcgtcttc gatgtcgagg gcggcttcgt cttgcggcaa acctttcggc
     9121 ggcgtgtgtc cgaacggcgt gttcggctcc atcgtcagat gatacgcgct gatgtgggtc
     9181 gcgcccgtgg cgatggcggt ttgcacgtcg tccgatgccg tctgaacggt ctggttcggc
     9241 agggcgtaca tcaagtcgat attgacttta tcaaacaatt tcagggcggt agctatggcg
     9301 gttaaggctt ctttgccgtt gtggacgcgc cccagccttg agagcatatc gtcgttgaag
     9361 ctttgtacac caatagaaag ccgcgtgatg cctgcgtctt taaatccttg aaacttctcg
     9421 atttcaaacg tccccggatt ggcttccaag gtaatttccg cttccggctg caagcgcaac
     9481 agcgaacgca cgccgctcaa caaacggtca atcgattccg cctgaaacag gctgggcgta
     9541 ccgccgccga aaaagatcgt ttccaccggc ctgccccaaa tattgggcaa ttcaagctgc
     9601 aagtcggtca gcagcgcgtc gatataggcg gcttcgggca atccgttttt caggctgtgg
     9661 gaattgaagt cgcaatacgg gcattttttg atgcaccacg ggatgtggat gtaaagcgac
     9721 aggggtggca gggcgatgag tcgggtgtgg tttggaaagg aaatggtgtg catggtgcgg
     9781 ttcggaaaag tgggcaatgc cgtctgaagg cggttcagac ggcatgggtt cagccgagca
     9841 gggtaagcag ttcggcttcg ctgaggacgg aaacgcccaa ggcattggct ttttccagct
     9901 tgctgcccgc ggcttctcca gcgacgacgt agtcggtttt tttggacacg ctgccggaaa
     9961 ctttgccgcc tgcggcttcg attagggatt gggcttggtc gcgtttgagg gtgggcagga
    10021 tgccggttaa cacgaaggtt ttgcctgcta cggctttatt gatgccgtct gaaccttgcg
    10081 ccgcctcgtc ttcagacggc atttgcgcga agaaggtttt caggttttcg agcagggcgg
    10141 cgttttgtgg ttcgctgcgc cacgcctgcc agtcggtggg gagggctttg tcggtttgca
    10201 gaccctctat acttttgccg gcgagttccc ataaggcttg ggctttgttt tcgctaattt
    10261 tgaagtcggg caggcgggcg atccagcgtt gcggttcggc atattgtgcg gcaggcaggc
    10321 tgacggcttg ggcttgcggg gcaacgcctg cggcgagcag ctcgtctatc atcgcctgct
    10381 gttcggcttg ggcgaagaag tgggcaatgg aatgcgccac taccgtgccg atgtcgggca
    10441 ggcaggcgag gacgggttcg ggggcgcggc ggacgcgttc caatgtgccg aatgcctgcg
    10501 ccagcgtttt ggcggtgcgt tcgccgacgt ggcggatgcc gagcgcgaac aggaagcgcg
    10561 cgagttcggg cgttttgccg gattctatgc ccccgaggat gttttccgcc cacttggtcg
    10621 gttgtttttt atgtttgccc gacgcgccga ccgaactgcc ttcagacggc atttgatccg
    10681 attcggcaac ggttttgtcc gccgtttcct tcattttttg cagggtcgga atatcgaggc
    10741 agtagaggtc ggcgaagtgg cggacgaggt cttgggcgac aagctgttcg atttgttttt
    10801 cacccaagcc gtcgatgtcc atcgctttgc gcgaggcgaa gtggattaag ccttgcgcgc
    10861 gttgtgcctg acaaagcata ccgccgctgc atcgggcgac ggcttcgcct tcttcgcgtt
    10921 cgatttcgct gcggcagatg gggcaatggg tcggcaggcg gtagggcttg tggagcggaa
    10981 cggattgggt ttgattagcg gacggtgttt cggcaaacaa atcgtcctgc cgatgcccga
    11041 tgccgtctga aacagcaacg gcggtttcct gcatcgggcg gcgttcaaaa atcacacgca
    11101 cgacttcggg aatcacgtct ccagcgcggc gcacgacgac ggtatcgccg acgcgcacgt
    11161 cttttcggga aacttcgtcc tgattgtgca gggtggcgtt ggtaacggtt acgccgccga
    11221 cgaatacggg ttgtaatcgg gcaaccggcg ttaccgcgcc cgtcctgccg atttgcacgt
    11281 caatcgcttc gacaatggtc agggcttctt cggcagggaa tttgtgggca atcgcccagc
    11341 gtggcgcgcg tgagatgaag ccgagttcgc gctgttgcgc caagctgttg actttgacca
    11401 ccataccgtc gatttcgtag ggcagttcgg ggcgtttttg ctgcatttgt tcgtaaaacg
    11461 ccaatacttc gtcgatattt ttgaaacagc cgaaattgcc atttggcaga ctgaagccga
    11521 gtgcttggaa ataggcgagt tcttggatgt gttcttccgc gacgaaaccg ccttgccggc
    11581 gggcgacgga gtaggggaaa aagtgcagtt tgcgttgcgc agtgatgcgc gaatcgagtt
    11641 ggcgtaggct gccggcggcg gcgttgcgcg gattggcaaa gggtttttgc ccgttttcgg
    11701 cttgtctttt attgagggcg gcaaaatcgg ctttttgcat cagtacttcg ccgcgtactt
    11761 cgatgagttc gggcgtattt tcgccgtgca gccgcaaggg gatgttggat acggttttga
    11821 tgttttgggt aacgtcttcg cccgttgtgc cgtcgccgcg cgttgccgcc tgtaccaata
    11881 cgccgtcgcg gtagagcagg ctgatggcga ggccgtcgaa tttaggttcg ataacgtatt
    11941 cgggtttgcc gccgtccaaa ccgtcgcgca cgcgttggtc gaaagcgtac atttctgcat
    12001 ggtcgaacac gccgttttca tcttgcgggg aaaaggcgtt ggtcagcgac agcatcggca
    12061 cttcgtggcg tacttcggca aatcccgcca gaggctcgcc gccgacgcgc tgggtcgggc
    12121 tgtcgggcag tttgagctcg ggatggtttc gttccaacgc ttcgagctcg cggaataatt
    12181 tgtcgtattc ggcatcgggt acgctgggcg cgtcgagggt gtagtattcg taggcatagc
    12241 ggttgagcag gtcggtgagg tggcggatgt gttgtgcggc aaattgtttt atatcactat
    12301 cagacggttt aagaagattg gtaaagttag tgttatgttt tgagtttgga ttcatgagag
    12361 aaggttttca gacgaccttt gtctgatacg ggatgaaacg ggcaaaggtc gtctgaaaaa
    12421 tgataggttg aaaacagctg aattttaccc gaaaaaaagc ggatatgccg taacgacata
    12481 tccgctttga ttgcattcga ttttaggaga acaggcgcag ggcggttttt cctccgggct
    12541 ggataccgac tttgagcatt tcctcctgac gtgccaaaac gtaggtgcgt acgtctttaa
    12601 gccattgggt agatacttct tccattttgt cgttaaccaa attgaggttc agttggccgg
    12661 acagtcggac tgccaaatcc ataaagaggt cgtcaaaagt tttttcgcct gccggggagt
    12721 gcggaatatc gagcagcata ctgaagcctt tgtaggactg gttgtccaaa agggcattgg
    12781 taaacggctc gttgttgagc gagcagatgg agaacatggt cgaacccgac gtgtcggtat
    12841 agtggaacgc gccgtcgtct tccaaaacga aacccacgcc cgttacggcg gaacgcagtt
    12901 ctacgccgct gatgctggtc ggggaaacca aatggatggc gatggtctga tcgacgcgcg
    12961 cgcagaatgc gtccagtgcg gaagccactt cgataaaggc ggcaaggtcg gtgtgcagcg
    13021 tctgaccacc catgctttgt gcgaatgcgt ccacctggcg gttgaatgcg gagagttctt
    13081 cctgcgaggc aagtccgttg cggctgactg cctgaatacc cacgataaat gcctgatagc
    13141 ggatgcccgg gatgggttcg gcaatctgga aatggtcgtc catggtgcag ccgacaatct
    13201 ggtagcggca gcggttggaa aggcgcggca gtgcgtgcag ttctttggct tcggtcagcg
    13261 cgatatagga gatgaagtcg aagcgcacgt caaaccaggg cagctcgacc ttcgacagct
    13321 ctttgagcgt aatcagcggt tttgtcggcg tttgcgggac aggaatgggt tttgccggcg
    13381 cgtcggcagg tttcgatgcg gaatgtccgg tttggggttc ggaaacggtg tgggcggagt
    13441 tgccgataat gccgctttct tccaaggcgg tttcgatttc ggttttgaac ggggaggctt
    13501 ttgcctgttt ctgcttggcg atgtagacgg catcctgttc ttgcaggttg cgcatggcgg
    13561 gatcttgggg ttttgccgtt tttttgaccg ccggttgggg tttcggcatc atgactggcc
    13621 cgccggacgg tttgccgtcg cggacatggc tggttttgct gttgagcagg gcatctttgt
    13681 cggagtgtcc gaactggtcg cgcacttttt tgcggtattg gttttcctga tacatattgt
    13741 aggcgacaac ggcgaggacg gcggcgagga acagtacgat gtaaatcatg gcaatcactt
    13801 taaatttcgg gatgcaggat acgcaaagtg cgggtactgc ggttaaatcg ggcttgcgtt
    13861 tccggcagtc tgacggaacg gccggttata acgtttgaat tataacgaaa attgcagggt
    13921 ctgacagcag tgtgtcgaaa taagcggaaa ttttccgaaa tgccgtctaa aatctgtggt
    13981 tttcagacgg catttctgtc cacgggaaac cctttccccc gtatccgccg ccagtcgaaa
    14041 aaatgaccgg ggtcggtttt gcggccgggc gcgatgtctt ggtgccccgt taccgccgtg
    14101 atggggtagc ggcggcagat tgcgtccaac aaggcttcga gcgaacggta ttgcgcttcg
    14161 gtaaagggtt cgaaatcgca gccttccagt tcgatgccga tggaaaatgc gttgcatttt
    14221 tcccgtccgc gaaacgagga tacgccggca tggtatgcca tattgtcgca ggaaacgaac
    14281 tgtaccgttt cgccgtcgcg tttgattaaa aaatggctgg atacgcgcaa agtgtgtatc
    14341 aggctgaaga acggatgtcc gtcggggtcg agccggttgg caaacagctt ttccaccgca
    14401 tccgtgccgt atttgaaggg cggcagcgaa atgttgtgca gcacgatcag ggaaaccgtt
    14461 tcccccgttt ccctcgggct gaaattgggc gacggggtat ggcgtatgct ttgaagccag
    14521 ccgttttgcc agtgtgcttc ggcgtgattg tccatgatgt tcttcctgtc cggcgggtaa
    14581 tttgggttat actgtcgccc gaattttaag acgtattccg aatgctggga atcctaccat
    14641 gttgaaaaaa atgttgaaat ggactgccgt ttttttgacc gtatcggcag ccgttttcgc
    14701 cgcgctgctt tttgttccta aggataacgg cagggcatac cgaatcaaaa ttgccaaaaa
    14761 tcagggtatt tcgtcggtcg gcaggaaact tgccgaagac cgcatcgtgt tcagcaggca
    14821 tgttttgacg gcggcggcct acgttttggg tgtgcacaac aggctgcata cggggacgta
    14881 cagattgcct tcggaagtgt ctgcttggga tatcttgcag aaaatgcgcg gcggcaggcc
    14941 ggattccgtt accgtgcaga ttatcgaagg ttcgcgtttt tcgcatatga ggaaagtcat
    15001 cgacgcaacg cccgacatcg aacacgacac caaaggctgg agcaatgaaa aactgatggc
    15061 ggaagttgcg cccgatgcct tcagcggcaa tcctgaaggg cagtttttcc ccgacagcta
    15121 cgaaatcgat gcgggcggca gtgatttgca gatttaccaa accgcctaca aggcgatgca
    15181 acgccgcctg aatgaggcat gggaaagcag gcaggacggg ctgccttata aaaaccctta
    15241 tgaaatgctg attatggcga gcctggtcga aaaggaaaca gggcatgaag ccgaccgcga
    15301 ccatgtcgct tccgtcttcg tcaaccgcct gaaaatcggt atgcgcctgc aaaccgaccc
    15361 gtccgtgatt tacggcatgg gtgcggcata caagggcaaa atccgtaaag ccgacctgcg
    15421 ccgcgacacg ccgtacaaca cctacacgcg cggcggtctg ccgccaaccc cgattgcgct
    15481 gcccggcaag gcggcacttg atgccgccgc ccatccgtcc ggcgaaaaat acctgtattt
    15541 cgtgtccaaa atggacggca cgggcttgag ccagttcagc catgatttga ccgaacacaa
    15601 tgccgccgtc cgcaaatata ttttgaaaaa ataaaccatg ccgtctgaaa agtttgtgtt
    15661 ttcagacggc atacccttac cggaactgca agcatgaaac cgcaattcat cactttggac
    15721 ggcatagacg gagccggaaa atccaccaac cttgccgtca tcaaagcatg gtttgaacgg
    15781 agggggctgc ccgtgctgtt cacgcgcgag ccgggcggaa cgccggtcgg tgaggccttg
    15841 cgcgaaatcc tgctcaaccc tgaaaccaaa gccggtttgc gtgcggaaac actgatgatg
    15901 ttcgccgcca gaatgcagca tattgaagac gtgatattgc ctgcactttc agacggcatc
    15961 catgtcgtgt ccgaccgttt taccgatgca accttcgcct atcagggcgg cgggcgggga
    16021 atgccgtctg aagacattga aattttggaa cattgggtgc agggcggttt gcgccccgat
    16081 ttgaccctgc tgttggatgt gccactcgaa gtgtcgatgg cgcgtatcgg gcagacgcgc
    16141 gagaaagacc gtttcgagca ggagcaggcg gatttcttta tgcgtgtgcg cagcgtttat
    16201 ctgaaccgtg ccgccgcctg tcccgaacgg tacgccatta tcgacagtaa ccgcagcttg
    16261 gatgaagtca gaaacaacat agaaaaagtg ttggacggac atttcggctg ctgatgcggc
    16321 aaatattgaa acaagcgcat ccgcccgcgc cgaaaatcaa acggcagtgc cgcaggtgaa
    16381 aatggcggta tgcgccaaac tttcggcatg atagaattac gctcggttac aaggcaggat
    16441 gcgtcggcaa tattaacgaa ccgcccgtaa catgatgacc cgaaagcgtt tcggacagtc
    16501 cgattcaaat ctttttctcg caacaggatt gacacatgga aaactcattg aaagaagccg
    16561 ccctcaagtt ccacgaatta cccgttccgg gcaaaatttc cgttaccccg accaaatctc
    16621 tggcgaccga caaagatttg gcgttggcgt actctccggg cgtagccgct ccttgtatgg
    16681 aaatccatgc cgatccgcaa aatgcctaca aatacactgc taaaggcaac ttggtcgccg
    16741 tcatttccaa cggtacggcc gttttgggct tgggcgacat cggcgcgctg gcgggcaaac
    16801 ccgtgatgga aggtaaaggc gtattgttca aaaaattcgc cggtgtggat gtgttcgaca
    16861 tcgaaatcga tgaaaaagac ccgcaaaaac tggtggatat catcgctgct ttagagccga
    16921 ccttcggcgg catcaacctc gaagacatca aagcacccga gtgtttctac atcgaacgcg
    16981 aattacgcaa acgctgcaaa atccccgtat tccacgatga ccagcacggc acggccatca
    17041 ttaccgccgc cgccgtattg aacgctctgc gttttaccgg ccgtaaaatc gaagaagcga
    17101 ccttggtttg ctctggtgcg ggtgcggccg cgattgcctg cttgaaccaa ttgctggatt
    17161 tgggcttgaa acgcgaaaac gtaaccgttt gcgactccaa aggcgtgatt taccaaaccc
    17221 gcgaagacaa agatcgcatg gacgaatcca aaaaattcta cgccgttgaa gacaatggcc
    17281 agcgcgtact tgccgatgcc gtcaaaggta aagacatctt cttgggcctc tccggcgcga
    17341 acctgctgac ccccgaaatg ttgaacacga tgaacgaaaa acccatcgtg ttcgccatgg
    17401 ccaacccgaa tccggaaatc ctgccgccgc tggcaaaaga aacccgtccg gacgtggtta
    17461 tcggtaccgg acgttcagac ttcccgaacc aagtcaacaa cgtattgtgc ttcccgttca
    17521 tcttccgcgg cgcgttggat gtcggcgcga ccaccatcaa cgaagaaatg aaacgcgcct
    17581 gcgtgtatgc tttggcggat ttggcgatgg aagaggtaac cgaagaagtg gttgccgctt
    17641 acggtaagaa atttgaattc ggcgcggaat acctgattcc gactccgttc gattcccgcc
    17701 tgctgccgcg cgtcgctacg gctgccgcca aagcagcgat ggaaagcggc gtggcaaccc
    17761 gtccgattgc cgactttgaa gcctacgccg ccaagctggg cgaatggaag ctgtaagccg
    17821 tttgtggtta aaatgccgtc tgaactgttt tcaggcggca ttttgctgtc agattgataa
    17881 atgaaagata ctggaaaatg aaagagatgg agactgtccg ttatcatatc ggcgatatgc
    17941 ccgagacttc aaaacaaacc gcccccgtca tgacgacagg gcagtgggtg ttgacgatga
    18001 ttgttttcat gattcctttg gtcaattttt gtttgggtgt tcggcagagg caacccgaac
    18061 cgcgccaatt tctgtaaagc gcagttgctt atttacctga ttggttcgct tatcggtttg
    18121 gtcttcgcgt tgtttatagg tgggtctgta tcgggtacgc atgattaatg ccccgggctg
    18181 attttgcttc gaggatttgt atcgaatatg ccgaattgtt tcaaatttca taccgttatc
    18241 gaacggcatt ggcaaaaacc ttatccggtt ttgtcttttc tgcttaagcc gctctccggg
    18301 ctgtttgcca aaattgcggc aaaacggcgg acggattttt tatcgggaaa acggcaaagc
    18361 gaaaagctgc ccgtgcctgt ggttgtggtc ggcaatatcc atgccggcgg tacaggaaaa
    18421 acgccgattg ttgccgcgct ggtgtcgggt ttgcaggaaa agggcgtcaa ggtcggcatc
    18481 atcagccgag gctacgggcg caagagcaag gcggttcatg tattgaatgc cgagagccga
    18541 gcggaagatg cgggcgatga gcctttgctg ctgttccgca aaaccggtgc gccgacggcg
    18601 gtgggcagca gccgtgcaga ggcaggcagg gcgttgctgg cggcgcatcc cgacatcgga
    18661 ctgattgtgg cggacgacgg tttgcagcat tacgccctgc ggcgagatgt ggaaatcgcg
    18721 gtgtttccgg cggcggatac ggggcgcacg gatttggact tgctgcccaa cggcagtttg
    18781 cgcgaacctt tgtcgcggct ggattcggtg gatacggtcg tcgtcagcgg cggcaaggcg
    18841 gatgcgctgt ttaggccgtc tgaaaatatg tttcacagcc gtatcgaagt gggacgaatt
    18901 taccgtttga acaatccgtc cgaaatactg gacacaggcc gtctgaaaaa tcaaaccgtc
    18961 gtcgccgtgg caggtattgc caagccggcg cgttttttcg attcgttgcg gaatatgggc
    19021 attaccgtga agcgaaccgt cgcgctgccc gaccacgccg acatttcggc ggcagatttg
    19081 cccgatgcgg acgcggtcat tattacggag aaagatgcgg tcaaattttc agacggcatt
    19141 tgcaccgata atgtttgggt gttgcccgtt tgtgcgataa tcgaacctga tttggcggcg
    19201 tttgtgttgg agcggttgga agatgtaccg aaggccgtct gaaagcacgg tttgggcgga
    19261 gtgattacgg atttgaataa gaacgcctcg cgccgtcatt cccgcgcagg cgggaatcta
    19321 agtctcgaat tttcaggaat gcctaggagg cttcagaaat cccaaatctc cggatttcca
    19381 cttggacagg aatgagaaaa ccggtcgtat tttttatctg cattaatcat tcattaaagg
    19441 attgaatatt aaactgaaaa ccttgttatt gcccttcgcc acgctggcat tgtgcaccaa
    19501 tgcttttgcc gctccgccca gcgacgcgtc gttggcgcgt tggctggata cgcagaattt
    19561 tgaccgggat atagaaaaaa atatgattga gggctttaat gccggattta aaccgtatgc
    19621 ggacaaagcc cttgccgaaa tgccggaagc gaaaaaagat caggcggcaa aagcctttaa
    19681 ccgctatcgt gagaatgttt tgaaagattt gattacgccc gaagtgaaac aggctgtccg
    19741 caatacttta ttgaagaatg cccgtgagat atacacgcaa gaagaaattg acggcatgat
    19801 tgccttttac ggttcgcccg ccggtcagtc cgtcgttgcc aaaaatccgc gcttaatcaa
    19861 gaaatcgatg agtgaaatag cggtatcttg gactgcattg tcagggaaaa tcgcgcgaca
    19921 tcatctgccc gagtttacgg aagagttgcg acgcatcatc tgcggcggta aaaatcccga
    19981 tgcgggctgc aagcaggccg gacagcttgg gaaaaggcat cagaaataaa tgatagccgt
    20041 ctgaaatatt gcagagggca tccgattgat taaaccatca aacccgaaag caaccttatg
    20101 gaaaaaaaat tcttagacat cctcgtctgc cccgttacca aaggcaggct ggaatatcat
    20161 caggacaaac aggaattgtg gagccgtcag gcgaagctgg cgtatccgat taaagacggc
    20221 atcccctata tgctggaaaa cgaagcgcga gcgttgggcg aagaggagct caaagtatga
    20281 ccgaattcgt cgtattgatt ccggcgcggc tggattcgtc gcgcctgccc ggaaaagcct
    20341 tggcggacat ccacggcaaa ccgatggtcg tgcgcgttgc cgaacaggcg gcaaaaagta
    20401 aagccgcgcg cgtcgtcgtc gccaccgacc atcccgatat tcagacggtc tgtcaggcgc
    20461 acggcatcga agcggttatg acttcaaacc ggcacgaaag cggcacgacg cgccttgccg
    20521 aagcctctgc cgcgctgaag ctgccgccgc atttgattgt tgtgaacgta cagggtgacg
    20581 agccgctgat tgcccccgaa ctcatcgacc gcaccgccga agtactcgtc gaaaacaacg
    20641 tccaaatggc gaccgccgcc cacgaattgc acgatttcga cgaattgatg aatcccaatg
    20701 ccgtcaaagt cgtcctcgac aaaaaccgca acgccatcta cttcagccgc gccccgattc
    20761 cctatctgcg cgatgcaatg cgtgccggaa aacgcgaaat gccgtctgaa accgccgtcc
    20821 tgcgacatat cggcatctac gcttaccgcg ccggcttcct gcaacgctat gccgaaatga
    20881 gcgtttcgcc gctggaaacc atcgaatcgc tggaacagct gcgcgtcctg tggcacggtt
    20941 atcccattgc cgtcgaaacc gccaaagaag cccccgccgc cggtgtggat acgcaagagg
    21001 acttggacag ggttcgcgcc gtatttcaga ccgtataaaa caggttcaaa gggaaaagat
    21061 atgcagcaac atattgaaaa gtggcaacac ttgagccggg aagaacagaa aatccttgct
    21121 gaagtatggg gtctcgtgca aaacgacgat caggaggttc actatgaaat gctcaaattg
    21181 aacgcacccg atgaagccag cggcgaattt tggttcagaa tggcagaaac actcagcacc
    21241 ctgccgccca accgttccct cggccttaga atgaacggcg gcaggctggc gaccgccgta
    21301 tccatccttt ccgtcatgat tgaagacaat cccgacatac cgcagctttg ggcgcaaaaa
    21361 attaccgcgc tcaattatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgt
    21421 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
    21481 atttggcaca cggacacaaa gcccgtgccg acggtttggc acaacagccc gacaaagcgg
    21541 cagaagccaa cgaggaggaa tacctgacca aagccctgtc gcaaaacctg ctgtcaacat
    21601 tggatgtcgc gcttgcacgt tttcctgaag acgcgtggtt tcagggaatc aaacaggatg
    21661 cacaaaagca ttttgcttga ggatgtggca gtcaggaata tttccattca ggaagaaaag
    21721 aagtgcctga ttgggtataa tcagggtaaa tcttatttta tttcaaaaga ttaatatttg
    21781 ctttctgttt ttccttgacg gtatcggaaa agttgattat agttacagct tccttaggag
    21841 taatggctga gaggctgaag gcacttccct gctaaggaag catgtggggt caacctgcat
    21901 cgagggttcg aatccctctt actccgccag ataaaaaata gacgctgtgt tttacagcgt
    21961 ctattttttt atgcaatttt atagcgggtt ggtgcaaaac cagtatggta ttgccctgtc
    22021 ttgattctga attttgttat agtggattaa caaaaaccag tacggcgttg cctcgcctta
    22081 gctcaaagag aacgattctc taaggtgctg gagcaccaag tgaatcggct ccgtactatt
    22141 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta taatccgaga
    22201 tgcttgccgt ttatttccgc ctcgttcaaa cggcggctct gatttgcgcg gtttctgttt
    22261 gccgtattcg cctatccgta ccgcaaatgt tatactggga aaaatttact gattgtgttt
    22321 tacggcatat ttgccgatag gatggaagag acaaatgagc agaatccggc aggcttttgc
    22381 cgctttggat ggcggaaagg cattgattcc ctatattacg gtgggcgacc ccgatattcg
    22441 gacaactttg gcattgatgc atggcatggt tgcaaacggt gcggatattt tagagttggg
    22501 cgtgccgttt tccgatccga tggcggacgg gccggttatt cagcgtgcgg cggagcgggc
    22561 gttggcaaac gggatttcgc tgcgcgatgt cttggatgtc gtcagaaaat tccgtgaaac
    22621 cgacacgcaa acgccggttg ttttgatggg atatttgaac cctgtacata agatgggtta
    22681 tcgggagttt gctcaggaag ccgcaaaggc gggtgtggac ggcgtgttga cggtggattc
    22741 ccctgtcgaa accatcgatc cgctctatcg cgagctgaag gataacgggg tcgactgtat
    22801 tttcctgatt gcgccgacga cgacggaaga ccgtattaaa accattgccg agctggcagg
    22861 cggatttgtc tattatgttt cgctcaaggg cgtaacgggc gcggcaagtt tggatacgga
    22921 tgaggtttcg cgtaaaatag agtatttgca tcagtatatc gatattccca tcggtgtcgg
    22981 tttcggcatc agcaatgcgg aaagcgcgcg caaaatcggc cgggttgccg acgcggtcat
    23041 tgtcggcagc cggattgtga aagagattga aaacaatgcg ggcaacgagg ctgccgccgt
    23101 cggtgctttg gtcaaagagt tgaaggatgc cgtgcgctga ttctgtcacg cattctaaat
    23161 attttaggag ttgtccatga gctggttaga taaaatcctg ccacccaaaa tcaaaaaccg
    23221 cagcaaagac ggttcttcca atgttcccga gggtttgtgg cacaaatgtc cgtcttgttc
    23281 ggcaaccgtt tattcgaccg agttgcagca gaacaatcag gtttgtccga aatgcaacca
    23341 ccacaatccg ttgtcggcac gacaacgcct gaatctgctt ttggatgagg aaggcaggga
    23401 ggaagttgcg ggtaatgtca aaccgataga tcctttgaag tttaaagacg gcaaaaaata
    23461 tccggatcgt ttgagtgcgg cacgcaagct gaccggggaa gatgatgctt tggtggtgat
    23521 gaaaggcaag atgaacggtc tgcccgtcgt cgttgccgcg tttgaattcc gctttatcgg
    23581 cggttcgatg ggttcggttg tgggcgaacg tttcgtacaa ggtatccgtc gggcggttgc
    23641 cgacaattgt ccgttcgtct gtgtggcggc ctccggcggt gcgcgtatgc aggagggtgt
    23701 gaactcattg atgcagatga cgaaaaccag tgccgcgctg catttgctga cggaaaaacg
    23761 cctgccattt atatcggtgt tgaccgatcc gactatgggc ggcgtatccg ccagcttcgc
    23821 atttttgggc gatgtcgtgc ttgccgaacc gaacgcgctg atcggttttg ccggtccgcg
    23881 cgtgattgag cagacggtgc gcgaaacgct gccggaaggc ttccaacgcg ccgagttcct
    23941 gctggaaaaa ggcgcaatcg accagattgt cgaccgccgc gatatgaagc ggcgcatcag
    24001 tgatttgatt acgctgttgt gccgtcagga caaagtttcc gccgcttgat ggctgatgaa
    24061 tcgaataccg tctgaaaccg atgtttcaga cggtattttt gtgtctggtt atttgttgtg
    24121 cggctttatc gacggggcat agcgtccggc acgttctttc aggcgttgta ccaaaccttt
    24181 cgtgtcggcg ggtacaccgc cctcgcagaa tgcctgatac aggacggtgc gcagtgcgtc
    24241 gttgcggctt aatgtaccgc ctatcggttt ccattcggcg tttcggggct gtatccagcg
    24301 gcggttgacc gtgtcgccgt agccgaacac tttataggag gaaggtttgc cgttgccgaa
    24361 cctgatggag aagcgggcgc agaatatgcc ttcggcagtc aggttgtcgt agcctttgtc
    24421 ggagcggata ttgagaatgt agcggatgct gccgtcgggc gcgggcataa tttgcaggct
    24481 gtcgagcagg attttcggct gtttgccgta attttcatcc acataaatgt caaaccagcc
    24541 gtccgagtgc gtatcgggca gcggcggcag tttggcggta tgttctttaa attcgcgggc
    24601 ggcggcttct tcgggcgttt cgcggtagcg ggtgttgatc ggcgtgtctt tttggttgaa
    24661 gccggcagca agggacgtgc cggcggtgag tgcgagcagc aggagggcgg tgcggcgcat
    24721 aagtttctcc aaattgaaaa cggcgttatt ttatgggttg gcaaaggggg ctgcaagcaa
    24781 ctggggtata atctcccccc gattcccatt ttttaaacgg tacaaacgat gaacagcgaa
    24841 actttagacg taaccggatt gaaatgtccc ctgccgattt tgcgggcgaa aaaggctttg
    24901 gcgcaaatgc agcagggcga cgtgttgacc gttctggcaa ccgacggcgg cgcaccgggg
    24961 gattttgagg ctttttgccg ccaaaccggt catgtgctgt tggatgcttc cgaacaggac
    25021 ggcgtgttca cgctggtcgt caagcacaaa taaatgccgt ctgaaatgcg gatgtcccgc
    25081 ccgacggcgt tgtttttgaa tatcgtatgt gccgcgtgcc gtttcaaaaa caaaccgtcc
    25141 ggcgtgcagt gtcggcattt cgggcgtatc gccgccgatt tgttccggaa tggctgttaa
    25201 ccgccttgcc gtcggcaaag aagcaaaacc caagcacaat caaaatctaa aggctgtgtt
    25261 tgaagattcc gttgatgcaa cccaatcagt cggacaaaat gcctttcatc caatggaacc
    25321 ggtttccgac cggacggaat aaccggcttt cccccggcaa acggatggaa tcgaccgggt
    25381 attcaaacgc agccaaaacc taaaaaggaa caaccatgca aaccctgacc atcatccgcc
    25441 ccgacgatat gcacctgcat ctgcgcgacg gcgacgcgct caaagccgtt gctccttata
    25501 ccgcccgcca aatggggcgt gccgtcatta tgcccaacct caaaccgcct gtcgtcagcg
    25561 ttgccgacgc gcttgcctac aaagcgcgca tcatggcggc attgcccgaa ggcagcgcgt
    25621 ttgagccgtt gatgacgctt tacctgaccg accaagccac gcctgcactt gtgcgcgaag
    25681 ccaaagccgc cggcatcgtc gccttcaaac tctaccccgc cggcgcgacg accaattccg
    25741 attccggcgt aaccgaccta ttcaagctca tccccgtgtt ggaagaaatg gcaaaacagg
    25801 acatcctgtt cctcgttcac ggcgaagtaa ccgaccccga aatcgacatt ttcgaccgcg
    25861 aagccgcctt tatcgagcgc gtgatgaaac ccgttttggc gcaagtgccg aatcttaaag
    25921 tcgtgtttga acacattacc accgccgaag ccgcccgcct ggttttggaa gcaggcgaca
    25981 acgttgccgc ctctgtgacc ccgcaacacc tcctgctcaa ccgtaacgac ctcttggtcg
    26041 gcggcgtgcg cccccatcat ttctgcctgc ccgtcctcaa gcgcgaaacc caccgtcagg
    26101 cattggttgc cgccgttacc ggcgagaagg cgcacaaatt cttcctcggc acagattccg
    26161 cgccgcacgc caaatccgct aaagaaaacg cctgcggttg cgccggtatg ttcagcgcga
    26221 tgaccgccat cgagctttac gccgaagtgt ttgaaaaagc aggcgcgttg gacaaactcg
    26281 aagcctttgc ctcgaaaaac ggcgcaaggt tttacggcat tccggaaaac gccggcacga
    26341 tcaccctcgt caaacaaagc caaaccgtcc ccgcaagtgt tccctacggc gacggcgaac
    26401 ttgtcccgat gcgcgcgggc ggcgaaatcg gctggacggt acagtattga ttgaacggtc
    26461 aagcaaaatg ccgtctgaaa ggtttttcag acggcatttg tgttgttgac tgattgatac
    26521 gtcaacggcg tttgagtttg ttacgtttcg gttatttccg ataaattccc acaattttca
    26581 aatttcgcca ttcccacgaa ggcaggaatc cggaaattcg atgcgaccag agtttatcaa
    26641 aaacggcagc aactcaaaaa accggattcc cgcctgcgcg ggaatgacga gattgaagtt
    26701 tcagaattta tttgaaatac ccaaaattca aaaaaccaaa ttcccacatg cgtgggaatg
    26761 acgaaacaaa gaaagcagaa ataaggacat agaactttct ttaaatttgt gatgcatcaa
    26821 cggcgtttgg gctcgtcggg gcggatttgg gcagcgagtt tgtcgaggat gccgttgacg
    26881 aatttgtgcc cgtccgtacc gccgaaggtt ttggtaactt cgatggcttc gttgataatg
    26941 acggggtagg gcgtttcggg tatggcggac agctcgtggc aggcggtcag caggacggcg
    27001 cgttcgatgg ggttgaggtc tttttcgtcc ctgtcgagca gcgggcggat ttgccggatg
    27061 tattctgccg cattggtttg cgtgccgaag aaaagtttgt tgaacaattc ttcgtctgcc
    27121 ttggcaaagt cggacatttc gcggatgttt ttagcaatct cgggtgctgc ggtgcggttg
    27181 ataagggatt ggtaaacggc ttgtacggca agctcgcggg aacggcggcg ggctgttttc
    27241 atgatttttc cttgaaacgg ttgggcggca cggtatgccg tctgaaacgg aaagggcgta
    27301 ttggtgtacg ccctgtttgt tattcttcgt cttcaaactg ttcttcgagc agcaggttga
    27361 cgaggttggc gcattctacg gcgactttgg cggcatccga ggctttttct tcaatccgtt
    27421 cgattgcctg cgcgtcgttt tcggtggtta agacggcatt ggcaatcggg atgttgtagt
    27481 cgagtgcgac gcggctaacc cctgctccgg attcgttgga aaccagctcg aaatggtagg
    27541 tttcaccacg gatgacgaca ccgatggcaa tcagtgcgtc aaatttttcg gaagaggcaa
    27601 gattcatcag cgcgatgggg atttcaagcg cgccgggtac ggtggcgacg gtaatgtttt
    27661 cgtctgccac gcccaattct tggaggatgc ggcagcagac tttgagcatt tcgctgccga
    27721 tttcgttggt gaagcgtgcc tgtacgatgc cgatgcggag gtgtttgccg tcgaggttgg
    27781 gggcgatggt gttcattggg tgtcctttgg tattcggagg tttcggaatg ccgtctgaag
    27841 gtttcagtct tgcggctgcc agtcggcgac ggtttggaat gtgccgtctt cggcaagctc
    27901 ccatgcgctg ccttcgggtt gggaaagcag tgcggcggtt tcagggttgg ttttggcgat
    27961 gtcggcgagg ctgacgatgc tgaagttgtc cggatcgtcg gtgtattcgt cggtctcgtc
    28021 gccgctgaag aaacgccagc cgctgtcgtt ttcaaaaacg ggggcttcgc ggtagaggaa
    28081 gccgacgggc cggttttgtt tggcgacggt gttggtggcg atacagcggt cgagtgccga
    28141 ggaaagtgct tgtgcaaatg cgttcattac gggaatacgt tgggggaaaa cttacggatt
    28201 ttaccacgat tcgtgcgttg tcggcagacg gcggcggttt ggtggtacaa tgtgcgccgt
    28261 ttgcagcctt aaggtgtttc tgtatttttg gagtatggaa acgcattcgg gctgtttttt
    28321 gcggaagacg gtaatgaaag acgatgtttt gaaacagcag gcacacgcgg cgatacagaa
    28381 gaaactgggc tatgcgttcc gcgatatttc gcttttgcgg caggctttga cgcacaggag
    28441 ccatcatgcg aagcacaacg agcggtttga gtttgtcggc gattcgattt tgaattatac
    28501 ggtggcgcgg atgctgtttg acgcgtttcc gaagttgacc gagggcgagt tgtcgcggtt
    28561 gcgggcaagt ctggtcaatg agggcgtgct ggcggaaatg gcggcggaaa tgaatgtcgg
    28621 cgacggtctg tacttggggg cgggcgagtt gaagagcggc ggcttcagac ggccttcgat
    28681 actggcggac gcgatggagg cgatgtttgc ggcggtcagc ttcgatgccg atttcaacac
    28741 ggcggaaaag gtggtgcgcc atttgtttgc cgaccgcgtc cggtgtgccg atttccaaaa
    28801 tcaggcaaaa gacggcaaaa ctgctttgca ggaggcgttg caggcacgcc gctttgcttt
    28861 gccgaaatac cgtatcgaag agcaaatcgg ccatgccaac gacagtatgt ttgtggtttc
    28921 ctgcgatttg ggcgaactgg gtttcgtgtg ccgtgccaaa gggacgagcc gcaaggcggc
    28981 ggaacaggag gcggcgaaag aggctttgaa atggctggaa gagaagctgc cgctgaagaa
    29041 gaaaaagaaa tgaggcggcg cgtgaatatg ccgtctgaaa tgtggatatg aaagcgaata
    29101 tggatattga aaccttcctt gcaggggaac gcgccgccgg cggataccgt tgcggcttcg
    29161 tggcgattgt cggtcgtccg aacgtgggca aatcaacgct gatgaaccat ctcatcggtc
    29221 agaaaatcag catcaccagc aaaaaggcgc agacgacgcg caaccgcgta acggggattt
    29281 ataccgacga taccgcgcag ttcgtgtttg tcgatacgcc cggctttcaa accgaccacc
    29341 gcaacgcgct caacgacagg ctgaatcaaa acgttaccga ggcgcttggc ggcgtggatg
    29401 tggtggtttt cgtcgtggaa gcgatgcgct ttaccgatgc cgaccgtgtc gtgttgaaac
    29461 aactgcccaa gcacacgccg gtcattttag tggtcaacaa aatcgacaag gacaaggcga
    29521 aagaccgtta cgcgctggag gcgtttgttg cccaagtgcg cgccgaattt gaatttgcgg
    29581 cggcggaggc ggtcagtgcg aaacacggat tgcggattgc caacctgttg gagctgctca
    29641 agccgtatct gcccgaaagc gtgccgatgt atcccgaaga tatggttacg gacaaatcgg
    29701 cgcgtttttt agcgatggaa atcgtgcgtg aaaaattgtt ccgctatttg ggcgaggaat
    29761 tgccttatgc gatgaacgtc gaagtggagc agtttgaaga ggaagacggt ttgaaccgca
    29821 tctatatcgc cgttttggtc gataaggaaa gccaaaaggc gattttaatc ggcaaaggcg
    29881 gggagcgttt gaagaaaatt tccaccgaag cgcggttgga tatggaaaaa ctgtttgata
    29941 ccaaagtatt tttgaaggtc tgggtcaaag tcaaatccgg ttgggcggac gacatccgct
    30001 tcctgcgcga gctgggtttg tagtttttct tgctgaactt tacgcaaatg ccgtccgaac
    30061 aggtttcaga cggcattttg tttcaatcgg gaatatcttt gttaaaaacg ggttgatatt
    30121 atctgtgca
//
LOCUS       DAWFJW010000013        29854 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFJW010000013 DAWFJW010000000
VERSION     DAWFJW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.013"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(204..788)
                     /gene="rnhB"
                     /locus_tag="WGR19_000480"
     CDS             complement(204..788)
                     /gene="rnhB"
                     /locus_tag="WGR19_000480"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0798070.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAAGPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(856..2751)
                     /gene="mnmG"
                     /locus_tag="WGR19_000481"
     CDS             complement(856..2751)
                     /gene="mnmG"
                     /locus_tag="WGR19_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0798071.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLMDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3076..4503)
                     /locus_tag="WGR19_000482"
     CDS             complement(3076..4503)
                     /locus_tag="WGR19_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0798072.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4663..5652
                     /gene="pdxA"
                     /locus_tag="WGR19_000483"
     CDS             4663..5652
                     /gene="pdxA"
                     /locus_tag="WGR19_000483"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0798073.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAAEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRRERINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGSALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            6158..6403
                     /locus_tag="WGR19_000484"
     CDS             6158..6403
                     /locus_tag="WGR19_000484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798074.1"
                     /translation="MGIQTFGRRQYSKIIRKFEVLDSRFHGNDERLWESRPSGSGNIQ
                     RLSESLEVLDSRFHGNDGMLRESGLFGFGFFEVSGNF"
     gene            complement(6818..9577)
                     /locus_tag="WGR19_000485"
     CDS             complement(6818..9577)
                     /locus_tag="WGR19_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0798075.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENSKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
     gene            10070..11062
                     /locus_tag="WGR19_000486"
     CDS             10070..11062
                     /locus_tag="WGR19_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0798076.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSS
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            11110..12282
                     /gene="lpxB"
                     /locus_tag="WGR19_000487"
     CDS             11110..12282
                     /gene="lpxB"
                     /locus_tag="WGR19_000487"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0798077.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHSMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLKRYPAARFLLPAATAATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            12290..12535
                     /locus_tag="WGR19_000488"
     CDS             12290..12535
                     /locus_tag="WGR19_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798078.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            12537..12947
                     /locus_tag="WGR19_000489"
     CDS             12537..12947
                     /locus_tag="WGR19_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798079.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVNCVDYPDLP
                     QEIPMPNYSDWVKIKFKQFSYLKFVYGYATKNRDKDIDNTLELGELKQDDEILDYGGA
                     LEVIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            12965..13210
                     /locus_tag="WGR19_000490"
     CDS             12965..13210
                     /locus_tag="WGR19_000490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798080.1"
                     /translation="MPRRQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPLQIRTTH
                     RATLYVSSRVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(13207..14016)
                     /gene="dapB"
                     /locus_tag="WGR19_000491"
     CDS             complement(13207..14016)
                     /gene="dapB"
                     /locus_tag="WGR19_000491"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0798081.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(14026..14403)
                     /locus_tag="WGR19_000492"
     CDS             complement(14026..14403)
                     /locus_tag="WGR19_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0798082.1"
                     /translation="MNKTLILALSALLGLAACSAERASLFPSYKLKVIQGNEIDPRAA
                     AALRLGMTKDQVLLLLGSPLLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALKDRQNTDKP"
     gene            14622..15056
                     /gene="fur"
                     /locus_tag="WGR19_000493"
     CDS             14622..15056
                     /gene="fur"
                     /locus_tag="WGR19_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0798083.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            15126..15851
                     /locus_tag="WGR19_000494"
     CDS             15126..15851
                     /locus_tag="WGR19_000494"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0798084.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVVFPDRLHIPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(16105..17136)
                     /gene="gap"
                     /locus_tag="WGR19_000495"
     CDS             complement(16105..17136)
                     /gene="gap"
                     /locus_tag="WGR19_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0798085.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(17336..18175)
                     /locus_tag="WGR19_000496"
     CDS             complement(17336..18175)
                     /locus_tag="WGR19_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0798086.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDESACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            18324..20300
                     /locus_tag="WGR19_000497"
     CDS             18324..20300
                     /locus_tag="WGR19_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0798087.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKHKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            20370..20445
                     /locus_tag="WGR19_000498"
     tRNA            20370..20445
                     /locus_tag="WGR19_000498"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20403..20405,aa:Val,seq:tac)
     gene            20478..20554
                     /locus_tag="WGR19_000499"
     tRNA            20478..20554
                     /locus_tag="WGR19_000499"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20512..20514,aa:Asp,seq:gtc)
     gene            21127..21225
                     /locus_tag="WGR19_000500"
     CDS             21127..21225
                     /locus_tag="WGR19_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0798088.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(21296..22678)
                     /locus_tag="WGR19_000501"
                     /pseudo
     CDS             complement(21296..22678)
                     /locus_tag="WGR19_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
     gene            complement(22933..25323)
                     /gene="gyrB"
                     /locus_tag="WGR19_000502"
     CDS             complement(22933..25323)
                     /gene="gyrB"
                     /locus_tag="WGR19_000502"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0798089.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRIIDELVLHAMLHASPVDLSTAESA
                     DRSVAELSGLLDEKEVALERIEGHEGHQFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            25593..26465
                     /locus_tag="WGR19_000503"
     CDS             25593..26465
                     /locus_tag="WGR19_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0798090.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            26553..28589
                     /locus_tag="WGR19_000504"
     CDS             26553..28589
                     /locus_tag="WGR19_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0798091.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            28595..28800
                     /locus_tag="WGR19_000505"
                     /pseudo
     CDS             28595..28800
                     /locus_tag="WGR19_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694731.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29032..29598)
                     /locus_tag="WGR19_000506"
     CDS             complement(29032..29598)
                     /locus_tag="WGR19_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0798092.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKADDVREAKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMATTVMVTVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT         6112 a         7600 c         8936 g         7206 t
ORIGIN      
        1 cggtttggaa gttacctgaa attcaaaaaa aaaaaaaacg gaaaccggac ggattggatt
       61 cccgtctgct cgggaatgac ggattttagg tttctttttt gattttctgt ttttcgcagg
      121 aatgacggtt tgggttcttt ctctttggag ttgcgatgcc ggaaatgccg tctgaaggct
      181 tcagacggca tttttgtgcc ggtttaaaac aaggcctgct gcgcgagcag gtttctgacg
      241 ggggcgaagt cgcggcggtg ttcgggcagc acgccgtatt tttcgagggc ttccaaatgc
      301 tgcttcgtgc cgtaaccttt gtgtttgtcg aaaccgtatt ggggatggcg ttgcgccagt
      361 gcgtacattt ccgcatcgcg ggcggtcttt gccaaaacgg atgcggcgga tatttcgatg
      421 attttgctgt cgcctttgac gacggcttcg gcagggatgt tcaaatgttc gggaatgcgg
      481 ttgccgtcga tgaatatttt ttcgggacgc acagccaaac cgtcaacggc gcgtttcatc
      541 gcgagcatgg tggcgtgcag gatgttgagg ctggcgattt cttcgggccc ggcggcggcg
      601 acgtgccacg caaccgcctg attttttatc atttcggcaa gcgcgtcgcg ttttttctcg
      661 ctgagttttt tggagtcggt cagtccgggc aggtcgaata tttccggaag gatgacggcg
      721 gcggcaaaca cgctgccgac taaaggcccg cgccctgcct cgtccacgcc ggcggtcagt
      781 atgtgcatga tgtttcctgt cgggatggca cgatgccgtc tgaacggtgt ttcagacggc
      841 atcgcgccga tgtgtttatt tcgcgtcttt aaacccgcgc ttcaaatgca ccatcagcaa
      901 tgccactgcc gcaggggtta cgccggaaat gcggcttgcc tgcccgacgg tttcgggttt
      961 gtgctggttg agcttttgct gcacttctgc cgacaaacct ttaactttgc tgtaatcgat
     1021 atcctcgggc agctttaagg tttcgatgtc gcggcggctg tcgatttctt cgttttggcg
     1081 gtcgatatag ccttggtatt tgacttggat ttcgacttgt tcgaccactt cggcggagag
     1141 gttttcagac ggcatcgcgc cttcgagcgt catcagcgcg gcgtagtcga ggtttgggcg
     1201 gcgcaggagg tcgtgcaggt tggcttcgcg gctgagcttt tgcccgaata cgcggatttg
     1261 ttcgtcttcg gcgagttttt gcggcgtgta ccacgttgtt ttcaaacgtt ggatttcgcg
     1321 ttcgatggct tcgcgttttt cgttgaacat gcgccattgc gcttcaccca ccaagccgat
     1381 tttgtagccg tcttcggtca ggcgcatatc ggcgttgtct tccctgagtt gcaggcggta
     1441 ttcggcacgg ctggtgaaca ttcggtaggg ttcgttcacg cctttggtga tgaggtcgtc
     1501 catcaatacg ccgagatagg cttgttcgcg gcgcagcagg agcgggtctt gttcgcggac
     1561 gtattgcacg gcgttcgcgc ctgccagcag tccttgcgcg gcggcttctt cgtagcccgt
     1621 tgtgccgttg atttgtccgg cgaagaagag gccttggatg gttttggttt cgaggctggc
     1681 tttgaggttg cgcgggtcga agtagtcgta ttcgatggcg tagccggggc gcaggatatg
     1741 ggcgttttcc aaacctttca tactgcggac gagcgcgatt tggatgtcga acggcaggct
     1801 ggtggagata ccgttaggat agtattcgtg cgtggtcaga ccttcgggtt cgaggaaaat
     1861 ctggtggctg tctttgtcgg cgaagcggtt gattttgtct tcgatagacg gacaataacg
     1921 cggacccacg ccttcgattt tgccggtaaa catcgggctg cggtcgaagc ctgagcggat
     1981 gatgtcgtgg gtttgcgtgt tggtatgcgt aatccagcag gacacttggc gcgggtgcat
     2041 ttcggcgttg ccgcgcacgg acatgacggg aacgggtgtg tcgccgggct gttcggtcag
     2101 ttgggaaaag tcaatcgtgc gtccgtcgat gcgcggcggc gtgccggttt tcagacggcc
     2161 ttgcggcagc ttcaattcgc gcaaacgtcc gcccagcgat ttggcggcgg ggtcgccggc
     2221 gcgtccgccc tcgtagtttt ccaaaccgat gtggattttg ccggacaaaa acgtgcctgc
     2281 ggtcagcacc acggcgcgtg ctttaaactc cacgcccatc gcggtgatta cgccgctgat
     2341 gcgttcgccg tcgagcgtta cgtcttcgac ggcttgttgg aaaaggtcga ggttttcttg
     2401 gttttccaac atttcgcgga tggcggcttt gtacaaaatg cggtcggcct gcgcgcgcgt
     2461 ggctcgaact gccgcgcctt tgctggcgtt caggcggcgg aactggatac cggatttgtc
     2521 ggtggccaac gccatcgcgc cgccgagcgc gtcgagttcg cgcaccaaat gccctttgcc
     2581 gatgccgccg atggaagggt tgcacgacat ttgtccgagc gtttcaatat tgtgtgtgag
     2641 caaaagcgtc tgcgcgccca tacgggcggc ggcgagcgcg gcttctgtgc cggcgtgtcc
     2701 gccgccgacg acgataacgt cgtaggtttt ggggtaaatc atgtgggtca tgtgtgtatt
     2761 gcctgtcggt gtttcagacg gcatttatag tggattaaca aaaaccagta cagcgttgcc
     2821 tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gtaccaagtg aatcggtttc
     2881 gtactgcttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgttg aaccactata
     2941 ttcaatatgc cgtctgaaaa acgaaatgga ttcaaaagta aagggttggg attgtacgct
     3001 tgttcgccct gtttttacag tgtgcggaaa gggaaaagcc gcttcgcggg gaagcggctc
     3061 cggtaagggc gggatttacc aaacgtcgga tttgatacgg cgtttcaggc ccggatgttc
     3121 ggaaagtttg aactcggggt ctttgcccat tttcagcttg gcggtgtaat cgcgcagcag
     3181 cataaacgcc aagggcgaga gcagcaggat ggcgacaagg ttgatccacg ccataatgcc
     3241 catcgccata tccgccatat cccagaccaa aggcacattg gcaaccgcgc cgaaatagac
     3301 ccacgccaaa accagcatac ggaaaacggc ggtaatcagc caatggcttt tgatgaattg
     3361 gacgttggac tcggcatagg catagttgcc gataacggtg gaaaaggcaa acataaacag
     3421 gatgacggcg aggaagcccg cgccccattg ccccacttgg ctgacaatcg ccgcctgcgt
     3481 cagcgccgca ccgctcaaat cgccgtaagg ctgttggtaa atcaagatga tgaaggcggt
     3541 gcaagaacaa acgatgatgg tatcgacaaa cacgcccagc atttgaatca taccttgcga
     3601 aacagggtgt ttcacttcgg cggcggcggc ggcgttcggc gcggaaccca tacccgcctc
     3661 gttggaatac aggccgcgtt tgatgcccat catcatcgtt tgcgaaatca gaccgccgag
     3721 taagccgcct gctgccgcgt cgaatttgaa cgcgcccgaa aaaatctgac cgaacacgtc
     3781 cggaatcatc ggaatattgg tcaaaatgat gaaaagcgcg ataaagaggt acaaaaccgc
     3841 catcaggggg acgacgattt ccgccgcttt agatatgcgc ctgatgccgc cgaagataat
     3901 cggcgcggtt aaaatcacca gggcgacgcc gacataatga ggctcccaac cccacgccgc
     3961 tttgacggta tcggcaatgg tattggtctg aaccgcttca aacacaaagc cgaaacagaa
     4021 aatcaggctc agggcgaaca acacgcccag ccatttctgc cccagccctt gagtgatgta
     4081 gtaggcaggg ccgccccgga aatggtggtt gtcgtagtcg cggactttaa agagctgcgc
     4141 cagcgaagat tcgacaaacg ccgaactcat accgattaag gcggttaccc acatccaaaa
     4201 caccgcgccc ggcccgccga ctttgatggc gattgccacg cccgcgatat tgcccacgcc
     4261 cacgcggctg gcaaggccgg ttacaaatgc ctgaaacggc gtgatgccgt gagggtcgtc
     4321 cccctgtttg cggccgccga gcatttcttt gatgctgcgc ccgaacaggc ggaattggac
     4381 aaagcccgtg gttacggtga agaaaagccc cgtacccaaa agcatataaa ccaagtatga
     4441 ccacatcgga tcgttgatgg cgccgaccca gccgtgcagc cattcggtaa agttctcgtt
     4501 catatcgctt ccttaaagtt gaaactcgca catattggcg gtatgcaagc agggtttaaa
     4561 ttttgtaaac gcccattcta gcagattgtc aacaaaatca gaaaaattta catcgccgcg
     4621 cggctgcggc gttagaatcg cattttgttt ggagcaaaca cgatgaaaca gcctgttttt
     4681 gccgttactt ccggcgagcc tgccggcatc ggccccgata tttgtttgga cttggcgttt
     4741 gcacgcctgc cctgccgctg cgcggtattg ggcgacaaac acctgttgcg cgcgcgcgcc
     4801 gaagccttgg gcaaaagcgt cgtcctgcgc gacttcgatc cagaatcagg cggcgcggca
     4861 tacggcgagc tggaagtgct gcacatccct gccgccgaag cggttgaggc gggcaaactc
     4921 aatcccgcca acgccgccta tgtgctgcaa cttttggaca ccgcgctcgc aggcatttca
     4981 gacggcattt tcgacggcat cgttaccgcg ccgctgcaca aaggcatcat caacgacgcg
     5041 cgcgcaagca caggtttttt cagcggacac accgaatatc tggcggaaaa aagcggcacg
     5101 gggcaggtcg tgatgatgct tgccggcaaa ggcctgcgcg tcgccctcgt aacgacccac
     5161 ctgccgctga aagacgttgc cgccgccatc acgcaaccgc tgattgaaag cgtcgcacgc
     5221 attttgcatc acgacttaaa acacaaattc tgcatcaaaa atcccaaaat cctagtcgcc
     5281 ggactcaacc cccacgccgg tgagggcgga cacctcggac acgaagaaac cgacaccatt
     5341 atccccgcgc tcgaaaacct gcgccgcgaa aggataaacc tcgccggccc gtatccggcg
     5401 gacacattgt tccaaccgtt tatgctcgaa ggtgcggatg ccgtattggc gatgtaccac
     5461 gaccaagggc tgcccgtgtt gaaataccac agtttcgggc agggcgtgaa catcacactg
     5521 gggctgccct ttatccgcac ctccgtcgat cacggcagcg cgctcgattt ggcggcaacc
     5581 ggcagggcgg attccggcag cctgataact gccgtggaga ccgccgtaga aatggcgcgc
     5641 ggcagccttt aaagatgata aaagacccgt catttccgcg caggcggcat cgtccaccct
     5701 ccgccgtcat tcccgcgcag gcgggaatcc agcctcgtcg gtacggaaac ttatcggaaa
     5761 aaacagtttc tccaaccctg cgttctagat tcccgctttt gcgggaatga cgaatttagg
     5821 tttctgtttt tggttttctg tccttgtggg aatgacgaat ttaggtttct gtttttggtt
     5881 ttctgtcctt gtgggaatga tgaaatttta agttttagga atttatcgga aaaaacagaa
     5941 accgctccgc cgtcattccc gcgcaggcgg gaatctagac ctttgggcgg cggcaatatt
     6001 caaagattat ctgaaagttt gaggttctag attcccgttt tcacgggaat gacggaatgt
     6061 tgcgggaatc cagaccttcg ggcggcggca atattcaaag attatctgaa agtttagagg
     6121 ttctagattc ccgttttcac gggaatgacg gaatgttgtg ggaatccaga cctttgggcg
     6181 gcggcaatat tcaaagatta tccgaaagtt tgaggttcta gattcccgtt ttcacgggaa
     6241 tgacgaaagg ttgtgggaat ccagaccttc gggcagcggc aatattcaaa gattatctga
     6301 aagtttagag gttctagatt cccgttttca cgggaatgac ggaatgttgc gggaatccgg
     6361 cttgttcggt ttcggttttt ttgaggtttc gggcaacttc taaaccgtca ttcccgcgca
     6421 ggcgggaatc cagaccattg gacagcggca atattcaaag gttatctgaa agtttgaggt
     6481 tctagattcc cgttttcacg ggaatgacgg aatgttgcgg gaatctggct tgttcggttt
     6541 cggttttttt tgagatttcg ggcaacttct aaaccgtcat tcccgcgcag gcgggaatcc
     6601 aggcctttgg gcgacggcaa tattcaaaga ttatccgaaa gtttgaggtt ctagattccc
     6661 gttttcacgg gaatgacgga atgttgcggg aatcatggga atgacgaaag gttgcgggaa
     6721 tgacgggaat ggcgaagttt cagacggcat tgcaggtatc cgaacccatg taaaaaagag
     6781 gttctgcgga acagaacctc tttttgccgc cgtcggttca gccttgccgg gtttcgactt
     6841 ggatcatttc ttcggcaggg acggttgcga cttcagacgg cttgggctgt tcggaacggc
     6901 gcaaaccgcg tccggcttgg acttcgggtt gtgccgccca tgccttcaat gcggcagggt
     6961 cggtttcgat caggacgagt ccgccggttt gtgcggtttc ccgtgcctgt tccgccgcag
     7021 ccgtaaaggt tgcggtttca gacggcattt cctgtgcttc ggctttcggt gtcgcgcctt
     7081 cgggcaggat ggcggcggtg gcacggcgga ttttttccgc cgcatcataa accggtgcgt
     7141 cgccgtttga aacggcggga gatgctgtcg gaagatccct ttctgcaacc ggatcggcaa
     7201 tgctgacggt aatcggcgcg tttgcgtcgg tttcgccgaa aacgtgcgcg gcggcggaac
     7261 ggactttgtc ggcggtatcg tgaatattca ggtactgctc gattttggcg gcagacggaa
     7321 tattgcgttt tttgccgttt tgacggcggt cgcgctgatt gttgcgctcg cggcgttctt
     7381 tggcatctcg gctgtcgcgt tcgcggcggt tgcgttcgga tttgggcttg ctgcctttgt
     7441 cttctgcggt atgcggttcg gacggcgtgt tttccgctgt ctgaacggtt gtttcggcaa
     7501 cggtggcggt ttccggcgcg gtttggccgc gttcgctgcg gctgccgttg cggcggcgtt
     7561 ttccggtttg cacttcggtt tcggacggtg cggcatctgc aacggttgcg gcaggctgta
     7621 tgttgcggtc ttggatttcc gcttcgtcgg cgcgtccggc ggcacggtcg ccgcgttcgt
     7681 tgcggcggcg gttgccgttg ttgcgcgttt cggctttgtc ggcgcgcgct tcctgtccgg
     7741 cagttttgcc tgccacttcg cggacttcta ctttgctgcc ttcgcgtttg ctgcggcgcg
     7801 ggttttggcg gcggttgttg gcgcggctgc cgctgcggtt tgccgtgctg cgtttttcgg
     7861 aggtttcggc agcgggcgcg gcttgggttt cgctgccgcc gaaaatgcgt ttgagccaag
     7921 ctttgaagct gtcccaccag gaggtttttt tctcgggggc ggcagtcggg gcggggctgg
     7981 tgtggcgcac gcctttgacg gcgggttcgg ggcgggcggc tttggctttt tcgccgccga
     8041 acggtttggc ggattcgtct tcttcaggct cggcgacgcg cttgtagctc ggttcgccgt
     8101 cttcttctac gtcgtcggtg cggatgcggt tgatttcgta gtgcggattt tcgaggtgga
     8161 tgttcggaat caggacgacg ttgacatcca aacgctcttc catcgcaaac agctcggcgc
     8221 gtttttcgtt cagcaggaag gtggcgacat cgacgggcac ttgggcgcgc acttcgccgg
     8281 tgttgtcctt catcgcttct tcttgaatga tgcgcaaaac gtgcagggcg gtggattcga
     8341 tgccgcggat gacaccagtg ccagcgcagc gcggacaggc gacgtggctg ctttcaccca
     8401 aagccggttt caggcgttgg cggctcaatt ccaaaagtcc gaaacgggag agtttgccca
     8461 tctgcacgcg ggcgcggtct tttttgagcg cgtcgcgtag gacgttttcc acatcacgct
     8521 ggtgtttgga gttttccata tcgatgaagt cgatgacgac caagccgccc aagtcgcgca
     8581 ggcgcatttg tcgggcgact tcttcggcgg cttccatatt ggttttgaag gcggtgtctt
     8641 cgatgtccgc accgcgtgtg gcgcgtgcgg agttgacatc gatggagacg agggcttcgg
     8701 tgtggtcgat gacgattgcg ccgccggaag gcaggctgac gctgcgagaa aacgcgcttt
     8761 cgatttgatg ttcgatttgg aagcgggaaa acagcggcgt gtggtcttcg tagagtttca
     8821 gacggcctat attgcccggc atgacgtagc tcatgaactc ggcaacttgg tcgtaaactt
     8881 cttgattgtc caccaaaatc tcgccgatgt cggggcggaa atagtcgcgg atggcgcgga
     8941 tgagcagcga gctttccata aagagcaggt aagggtcgtg atgcgctttt cctgcttctt
     9001 caatcgcctg ccagagttgt ttgaggtagt tcaagtccca ttccaactct tccgcgctgc
     9061 ggccgatgcc ggcggtacgg gcgatgatgc tcatgccgtt tggaatgtcg agttccgcca
     9121 tggcggcttt caactcttga cgctcttcgc cttcgatacg gcgggatacg ccgccgccgc
     9181 gcgggttgtt cggcatcaat accagatagc gtccggcgag gctgatgaag gtggtcagtg
     9241 ccgcgccttt gttgccgcgc tcgtcttttt cgacttggac gatgacttcc ataccttctt
     9301 tgagcacgtc ctgaatacgc gccctgccgc cttcgtagtc gcggaaatac gaacgcgata
     9361 cttctttaaa cggcaagaag ccgtggcggt cggttccgta atccacgaaa cacgcttcca
     9421 gcgacggctc gatgcgggta atgatgcctt tgtagatatt gcctttgcgc tgttctttgc
     9481 ccagcgtttc gatgtccaaa tccagcaggt tttgtccgtc gacgatggca acgcgtagct
     9541 cttcggcctg cgttgcgttg aataacattc ttttcatgat cacctcgtgg acgggcggca
     9601 ttcgggcgcg ccggtccggt tcggcattcc gtaaggctgg gttttccgat gtcttcggat
     9661 aaaaccggta atcagttttt gagttgaaaa tccgcaggga tgcacgttcc ggagaaccgt
     9721 gtgcggaagg gtcggataca gaaggctata aagatcgatg cggcggtctg tctgccgcgt
     9781 tccgagcggt atggtcggaa aaatgggtgc cggcttcttc ttgttatcgt gatgcctgtg
     9841 ttttcgggcg gtttgcgttt ggggcttggg cccggctgcc gtcttacttc cgcgccgaaa
     9901 cggcaaaatc aattcaaact tgactacgtt ctgcgcctgc cggctgggaa caggcgcagg
     9961 gaaaatgctt tgcggagtgc gtttttaata taaaattccg ttttaaagta aaccgtttca
    10021 ggaggcgcgg cgggcgcgct ttttgctgaa acggatgttc ggattataga tgaaaacgca
    10081 cgaaataagc aaagattcgg tcagcttgat aggggtcgcc gaacatgagg cggggcaacg
    10141 ccttgataac tatctgataa aaatcctcaa gggcgtgccc aagagccata tccaccgcat
    10201 tatccgcgcc ggcgaggtgc ggttgaacaa gaaacgctgc aaacccgaca gccgtattgc
    10261 ggagggggat acggtgcgga ttccgcctgt gcgcgtggcg gagaaggaaa tgccgtctga
    10321 aaggcgtgcc gccgtaccgg cgcgtgcgtt tgacgttgtt tacgaagacg atgcgctttt
    10381 ggtcatcgac aaaccgtccg gcgttgccgt ccacggcggc agcggcgtga gtttcggcgt
    10441 tatcgaacag ttgcgccgcg cccgtccgga ggcgaagtat ttggagttgg ttcatcgttt
    10501 ggacaaggat acgagcggct tgttgatggt ggcgaagaaa cgcagctcgc tcgtcaaact
    10561 tcacgaagcc atccgtaacg accaccccaa aaaaatctac cttgcgctgg gggtgggcaa
    10621 actgccggac gacaatttcc atgtcaaact gcccctgttc aaatataccg gcgcacaagg
    10681 cgaaaagatg gtgcgcgtca gtgcggacgg gcagtcggcg catacggtgt tccgtgtgtt
    10741 aagccgtttt tcagacggca ttttgcacgg tgtcgggctg tcgcacctga ctttggtgcg
    10801 ggcgacgttg aaaacggggc gcacgcacca aatccgcgtc cacctgcaat ctcaaggctg
    10861 tccgattgcg ggcgacgaac gctacggcga ttatcaggcg aaccgtcgtt tgcagaagtt
    10921 gggtttgaag cggatgtttt tgcacgcgtc cgagctgcac ttgaaccatc cgctcacggg
    10981 tgagccgctg gtgttgaagg cggagctgcc gccggacttg gcgcagtttg cggtgatgtt
    11041 ggaaaacggg acgaaaatgt gaaccccgat gccgtctgaa gccttcagac ggcatcggga
    11101 cgtgaaagta tgtggggaca gacgaatatg gttgataaaa aaagcccttt gattgccgtc
    11161 agtgtcggcg aagcgtcggg cgacctgttg ggggcgcacc tgatacgcgc catccgcaag
    11221 cgttgcccgc aggcgcggtt taccggcatc ggcggggaac tgatgaaggc ggaaggtttc
    11281 gagagccttt atgatcagga gcggctggcg gtgcgcggct ttgtcgaagt ggtcaggcgg
    11341 ctgccggaaa ttttacggat acgcaggggg ctggtacggg atttgctgtc gttgaaacct
    11401 gatgtctttg tcggtatcga tgcgcccgat ttcaatttgg gtgtggcgga aaagctgaaa
    11461 cgggcgggca ttccgaccgt gcattatgtc agcccgtcgg tgtgggcgtg gcggcgggaa
    11521 cgcgtgggca aaatcgtgca tcaggtcaac cgcgtgctgt gcctgttccc gatggagccg
    11581 cagctttatc tcgatgcggg cggacgtgcg gagtttgtcg gtcattcgat ggcgcagctt
    11641 atgcccttgg aagacgaccg tgaaacggcg cggcaaactt tgggcgtgga tgccggcatc
    11701 cccgtattcg ccctgctgcc cggcagccgc gtcagcgaaa tcgactatat ggcgccggtg
    11761 ttttttcaga cggcattatt gttgttgaaa cgctatcccg ccgcacgctt cctgctgcct
    11821 gccgcaacgg cggcgacgaa gcggcgtttg gcggaaattt tgcagcgttc ggagtttgcc
    11881 ggattaccgc tgacggtaac cgacagacag tctgaaacgg tgtgcagggc ggcggatgcg
    11941 gtgctggtaa cgagcggtac ggcgactttg gaagtggcgc tgtgcaagcg tccgatggtc
    12001 atcagctaca agatttcgcc gctgacttat gcttatgtga aacgcaaaat caaagtgccg
    12061 catgttggcc tgccgaatat cctgttgggt aaggaggctg tgccggagct gcttcaacat
    12121 gatgccgtac cggaaaaact ggcggcggcg ttggcggact ggtacgaaca ccccgataag
    12181 gttgccgcgc tgcaacagga tttcagggcg ttgcacctgc tgttgaaaaa agatacggcg
    12241 gatttggccg cgcgcgcggt tttggaagag gcgggatgtt gagcggttaa tggattattt
    12301 tcccgaagca gcacgtatta caaaaaaagg gggagaaatt gtgattaatg gcacatcaaa
    12361 caataagtat ttaagaggaa ttccaaatga agcagaactg gctcgagtgg gattaaggtt
    12421 aaaatataat ggtctgttaa aggaagagtt taaacaactg aaatttcata agagtagtag
    12481 aaccaacttg gactcaaaaa atcttaaact cattgttttt gaaaaggtaa aataatatga
    12541 caacttatac cattccaaaa aaagattatc aatttctgta tatatatgag ggcactctat
    12601 taaactatac tttgaaaaac gatgaattcc atatcatcgt caattgtgtg gattatccgg
    12661 atttacctca agaaatccct atgcctaatt attcagactg ggtaaaaatt aaattcaagc
    12721 agttcagcta tttgaaattt gtctatggat acgccacaaa aaaccgagat aaagatatcg
    12781 ataatacatt ggaacttggc gaattaaagc aggatgatga aatcttggat tatggaggtg
    12841 cgctggaagt gataggcggt aggtatgatt ttccgactgc ttttaggata gatatagttt
    12901 gccgggaaat agagttagaa tttttagatc aggagggttt caattaaacg agccgtagct
    12961 tgttatgccg cgcaggcaac tttatcgtat ttccttttcg gttgaaaccc cgccactcgg
    13021 acatctgtcc ttcggggcgg tagaatcaga ctttatttgg gaggggcgta atcccttgca
    13081 aatcaggacg acacataggg cgacgcttta tgtatcgtcc cgtgtgttga aacatatgcc
    13141 gatacggagc ctgtcggcaa aatgccgtct gaacaatatc ttttcagacg gcattttgta
    13201 tgggggttaa cggttgttca gcccgagtac gtcctgcata tcgtacaaac ccgttttgcc
    13261 gtttacccaa actgcggcgc ggacggcgcc ggcggcaaag gtcatgcggc tgccggcttt
    13321 gtgggtgatt tccacgcgct cgccgtcggt ggcgaagagg gcggtgtggt cgccgacgat
    13381 gtcgcctgcg cggacggtgg caaagccgat ggtcgacgga tcgcgcggac cggtgtggcc
    13441 ttcgcggccg taaacggcgc attgtttgag gtctctgccg agcgcgccgg cgatgacttc
    13501 gcccatgcgt aacgcggtgc cgctgggggc atcgactttg tggcggtggt ggccttcaat
    13561 gatttcgatg tcgtagcctt cgtttaatac gcgtgcgacg gtgtcgagga tgtggaaggt
    13621 gaggttgacg ccgacgctga agttggcggc gaaaacgatg cctgtttgtt cggcggcagt
    13681 gtggatagcg gctttgcccg catcgtcgaa gcctgttgtg ccgatgatga tgttgacttg
    13741 tttttcaacg catttttgca ggtgtttgag ggtgggctcg gggcgggtga agtcgatgag
    13801 tacgtcgctt tgtgcgagaa cggcgtcaac gtcgtctgaa atggcgatgc cggttttgag
    13861 tccgacggcg tagcctgcgt ccagtccgag ggcttctgag cctgagtgtt cgagcgcgcc
    13921 ggaaaggacg gtgtcgggat ggttgttgac ggcttcaacc aatacgcgtc ccatacggcc
    13981 gtttgcgccg gcgatggcga ttttgagcgg tgtcatgtgt gttccttatg gtttgtctgt
    14041 gttttggcgg tctttgaggg cttcggcagc gttttgcagg gcgtcgcctt cggtgcggac
    14101 gagtacgccg ttttcaaaat agacggtcag attgctgcgt tctttgatga tgccgttgcg
    14161 ggaggtgttg aaggtatagt cccagcggtc ggtgtggaac gcgtcgcgca acagggggct
    14221 gccgagcagg agcaggactt ggtctttggt cataccgagg cggagtgcgg cggcggcgcg
    14281 ggggtcgatt tcgttgccct gtatgacttt gagcttgtat gaggggaaca gcgaggcgcg
    14341 ttcggcactg cacgcggcaa ggccaaggag ggcggaaagg gcgaggatga gggttttgtt
    14401 cacggaaatg cctttctgtg caaatcggga tgggtagtgt aacactgctt gaatatttta
    14461 taaaagcgaa cgataatcat acgattaagc ggtatccgcc ctgtccgcgc atcgaccgcc
    14521 ggtgcggttt tactattgca aactgctatg gtgcgatagt aggcaaacag gccgaaattg
    14581 cgtattataa cgtctattgt tttacagggg tattgaatat tatggaaaaa ttcaacaata
    14641 ttgcacaact gaaagacagc ggtctgaagg ttaccggccc gcgtttgaag attttggatt
    14701 tgttcgagac gcatgcggaa gagcatttga gtgcggaaga tgtgtaccgc attttgttgg
    14761 aagagggtgt ggaaatcggt gtggcgacga tttaccgcgt gctgacgcaa tttgagcagg
    14821 cgggcatttt gcaacgccac cattttgaaa cgggcaaggc ggtttatgag ttggacaagg
    14881 gcgaccacca cgaccatatc gtctgcgtga agtgcggcga ggtaacggag ttccacaatc
    14941 ccgaaatcga agccctgcaa gacaaaatcg ccgaggaaaa cggctaccgc atcgtcgatc
    15001 acgcgctgta tatgtacggc gtgtgcagcg actgtcaggc caagggcaaa cgttaaatcc
    15061 ggacggtttg ttgttcagac ggcattcatg attttggatg ccgtctgtgt ttttggagaa
    15121 ctgccatgcg tattccgctg cttgcccctg acaattatgc ctttcccgat cctacctatg
    15181 ctttggcccg gtgcgacggg ctggtcggcg tgagcggcga tttggatgcg gggcggctgc
    15241 tcgaggcgta tcggaacggc gtgtttccgt ggttttctcg ggacggttgg tttttttggt
    15301 atgcggtcgg gccccgtgcg gtggtgtttc ccgacaggct gcatattccg cgctcgctgg
    15361 cgaaaacgct gcgcaacggc agctatcggg ttgcggtcaa cggctgtttt gcggaagtgg
    15421 tcgcgcattg tgcggcagcg gcgcgcccga atcaggacgg aacttggatt gcgcccgagt
    15481 ttcagacggc atatttgaag ctgcacgaaa tgggacacgc gcattctttc gagtgccatt
    15541 atcccgatga aaacggtaaa acgaggttgg cgggcggctt ttacggcgtg caaatcggca
    15601 gggtgtttta cggcgaatcg atgttcgcat tacaaccgga tgcgtcgaaa atcgcgtttg
    15661 cctgcgccgt gccgtttttg gcggatttgg gtgtggaact gatagactgc cagcaggaca
    15721 cggaacatat gcgccgtttc ggttcggagc tgctgccgtt tgcggatttt gccgaacgtc
    15781 tgcggatgtt gaacgccgtg ccgttgaaag aggaaatcgg gcggcgcgaa gtggcgtgca
    15841 aggggctttg atggcggctt atgctccggt caggttcaaa tatggtggat tatagtggat
    15901 taacaaaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggcaaggcga
    15961 ggcaacgccg tactggtttt tgttaatcca ctataaaatt agaaatgacg acagccgggt
    16021 aaaatcacgg tgaaaaatga aaaatgccgt ctggaacttg aaaacatcgg gtttcagacg
    16081 gcattttgtt tgacggttta ttgcttattt gagcgggcgc acttcaagtc cgaacatacg
    16141 gcgtgcggtg ttcagcattt ggcagctgaa gccccattcg ttgtcatacc atgcgaagac
    16201 tttaatcatg ttgccgtcaa cgactttggt cagtgttgcg tcgaagtggc tggcttcggt
    16261 agtgtggttg aagtccatgg aaaccaaggg cagggtgttg tagcctaaaa cgcctttgag
    16321 cgcgcctgct tccgaggcgg ctttcatcag tgcgttgatt tcttcgactg tcgtgtcgcg
    16381 cgcggcttgg aagctcaaat ctaccaatga tacgttgacg gtcggcacgc ggatggcaag
    16441 cccgtcgagc ctgcctttca attcgggcag taccaaaccg acggcttttg ccgcgccggt
    16501 tttggtcgga atcatgtttt ccacgccgct gcgggcgcgg cgcaggtctt tgtggcgcac
    16561 gtcggtaacg gtttggtcgt tggtcagcgc gtggatggtg gtcatcgcgc ctttgacgat
    16621 gccgacgctt tcgctcaaca ctttggcaac cggcgagagg cagttggtgg tgcaggaagc
    16681 gttggaaacg acggtcatgt cggcggtcag gacgctgtcg ttcacgccgt acacgacggt
    16741 tgcatcgaca tcgtcgccgc ccggtgcgga aatgaggact ttttttgcgc cgctttccag
    16801 atgaactttg gctttttctt tgctggtgaa cgcgccggtg cattccatga ccaaatcgac
    16861 accgagttct ttccacggca gttcggcagg gttgcgggtc gagaagaagg ggattttgtc
    16921 gccgttgacg atgaggttgc cgccgtcgtg ggatacgtcg gcttcaaagc gtccgtgtac
    16981 ggtgtcgaat ttggtcagat gggcgttggt ttcaaggctg ccgctggcgt tgacggcgac
    17041 gatttggagt tggtcttgaa tctgatagtc gtagatggcg cgcaaaacct ggcggccgat
    17101 gcgtccgtag ccgttgatgg cgactttgat gcccatggtt cgttcctttg ttgaggggtt
    17161 gggtagattt tcggggcgga ttatagcaaa tttgtagtgg cgtgtaatta atattttatt
    17221 gaaaacagcg cggtcggaag ggtgggcggt aagatgcgga cggcacgggt gcggcggacg
    17281 gagagcttga taaaatgccg tctgaagcgg cttcagacgg catttcgggg agggctcagg
    17341 aggcggtatt ctgtgcggct tcctgtttgg ctttgtattg tttgagatat tcgagggcgg
    17401 cggctttttc gctgtcgctg ccgtatttca tatcgcgttg ggcgcggcgc aactcggcgc
    17461 gttcgcgggc ttcggctatc tgtttcgcct gatagtcttt gcggttgtcg gcggcggcga
    17521 ggcggtcttg ttgggtttgc gcttttgcca tggctttggc gatgaggtcg gcagggttaa
    17581 acgccggttt tttcggtgtg tcgggtgttt gcggacgcgc gttgcggacg gcggcttcgc
    17641 gttcggcaag catggccttg cgttcgtcgg cttcgcgctg tttgcgttcg ttgcgtttga
    17701 ggtagcgcgt gcgcgcgtgt tcggcggcgg caaaacggct gtcggcggac aggctgaagc
    17761 ggcgcgcgcg gggcaggacg gtgtcggcaa cgggctgcat atggatgcag tcgacggggc
    17821 agggggcgac gcagagtccg cagccggtgc attcgtcggc gatgacggtg tgcataagtt
    17881 tgcccgcgcc cataatggca tcggcagggc aggcgcggat acaggcggtg cagccgatac
    17941 aggcggattc gtctatccgg gcgagtgctt tggcttgggt tttggcaggt gcgacaaggg
    18001 gtttgccgag cagggcagca aggtcgcgga cgacggttgc gccgcccggg gcgcagaggt
    18061 tgtacgcttc gcctgttgcg actgcctgtg cgtagggcag gcagccgtcg tagccgcatt
    18121 cgcggcattg ggtttgggga agcaggcggt ctatggcggc ggctgtggcg gtcatgtcgg
    18181 tgtgcggctc aaaatcgaaa gggcgtattt tagcagaatt gtatgccgcg cccgtttcgg
    18241 atggtgcgcg gtgttttgtt ataatgcggc ggcgtatgcc gtttcagacg gcatttttct
    18301 gtattttcct gttcggacgg tctatgaacg aattttcgct tgcccctatt gtgattgttt
    18361 tgctggtgtc ggtcattacg gtgatcctgt gccgcaagtt caacattccc tccatgctgg
    18421 gctacctgct ggtgggcttt ttggcggggc ccggtatgct cagcctgatt ccgaaaagcc
    18481 atgcgacgga ttatttgggc gaaatcggga ttgtgttcct gatgttcagc atcggtttgg
    18541 agttctcgct gcccaagttg agggcgatga ggcggctggt gtttgggctg ggcggtttgc
    18601 aggtcggcgt tacgatgctg tcggtaatgg gcatactgat gctgacgggc gtcccgttca
    18661 attgggcgtt tgccgtgtcg ggcgcgttgg cgatgtcgtc cacggcgatt gtgagccgga
    18721 ttttgtcgga aaagacggaa ttggggcagc cgcacggtca gatggcgatg ggtgtgctgc
    18781 tgatgcagga catcgccgtc gtgccgctga tgattctgat tcccgcgctg gcgggcggag
    18841 gggacggaaa tatttgggcg gccttgggtt tggcgtttgc aaaaatgctg ctgacgctgg
    18901 ggctgctgtt tttcgtcggc agcaaaatta tgtcgcgatg gttcaggatg gtggcaaaac
    18961 acaaatcgtc cgaactcttt atgatcaatg tgctgctggt aaccttgggt gtggcttatc
    19021 tgactgagct ggaaggtttg tctatggcgt tgggcgcatt cgttgccggc atgctgcttt
    19081 cggaaacgga ataccgtttc caagtcgaag acgacatccg cccgttccgc gatatcttgc
    19141 tcggcttttt ctttatcacg gtcggcatga agctggacat tcaggcatta atcggcggct
    19201 ggcggcaggt attgatgctg ttggcaatgc tgctggtgtt gaaggcactg gttgtgtttg
    19261 ccattgcctt caaaatgaaa cattcggtcg gcgacagcct caaaacggct ttgtatctcg
    19321 cgcagggcgg cgagttcggc ttcgtgatgc tggccattgc cgggcagctt gatatggttt
    19381 cgccagaatg ggaacaggcg gcgacagcgg cggtgctgct gtcgatgatt atcgcgccct
    19441 tcctcttggg cggcagcgat gcgctggtcg ggcgtttggt caagtcaagc tgggacatga
    19501 agtcgctcga tctacacagt atgctggtag aaaccatgag caagtccgac catgtgctga
    19561 ttgtcggctt cggcaggggc gggcagacgg tcggacgcgt ccttgcccaa gaggatattc
    19621 cgtatttcgc gctcgacttg gacattgcgc gggtgcaggt tgccagaagt gcgggcgaac
    19681 cggtgtcgtt cggcgatgcg aaacgcaggg aagtattgga agccgccggt ctgggacggg
    19741 cgaaaatggt ggtggttacg ctcaacaata tgcacgaaac gcaacacgtt ttagacaatg
    19801 tgctgtccat gtatcccaat atgcccgtat atgtgcgcgc caccaacgac gattatgtga
    19861 aaacgtttac cgatataggt gcggaagaag ccgtgtcgga caccaaagaa accggactcg
    19921 tgctggcagg ctatgcgatg ttaggcaacg gcgcgtcgta tcggcacgtc tatcagacga
    19981 tggcaaatat ccgccacagc cgttatgccg cgttggaggg actgtttgtc ggtagtgatg
    20041 atgaggcagg attcggcgaa aacggcgaaa ccgtccgtca cgcctttcct ttggctgcag
    20101 aagcatacgc cgtcggcaaa acagtcggca cgcttccgat ggcggcttac ggcatcaaac
    20161 tcttgttcgt ccgccgccgc accggccgga ttgaaaaccc ggatgcctcg tttacattgg
    20221 aaggcggtga cgtgttggtg gtcgcaggca aaaaagaaga aattatctct tttgaaaact
    20281 ggagtttgca gggaatataa atgaaatgcc gaaataaggc ttgcgccatt tccggttatt
    20341 tggtttaata acgctttcgc aaatcgcaag ggtgattagc tcagttggta gagcgtctgc
    20401 cttacaagca gaatgtcggc ggttcgactc cgtcatcacc caccaagttt tctttcattg
    20461 ttgcaaacaa tggatgcgcg gtggtagctc agttggttag agtaccggcc tgtcacgccg
    20521 ggggtcgcgg gttcgagccc cgtccgccgc gccaaaagtt aaggaatacc aacctccggt
    20581 tggtattttt ttgtttgtat gctttgaaaa atgtttgttt ccggattttg ccattcccat
    20641 ccggttttgc gctgtacgat gtgttttagc gcggacttgc tcaaaatcgg atgtggttcc
    20701 ggtatttgag gctttgatta gggatgcgga ctttcaatat atttttctca gctacaacaa
    20761 cgaaggcttg atgtctgtcg ggcaggtaag ggagattttt gagtgtttcg gcaaatataa
    20821 tttggttcaa acggaatacc ggcgttttaa ggcagataag acagaaaacc gtaatcataa
    20881 ggcaaattcg atattcgaat atctgcatat tttagaaaag accttttata gtggattaac
    20941 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga
    21001 agcaccaagt gaatcggttc cgtactattt gtactgtctg cggcttcgtc gccttgtcct
    21061 gatttttgtt aatccactat aaaaattctt gccggatgct gcaaacaacg ccggtttgca
    21121 ttcctgatgg cggtggtttt cttagacgaa cgcccgaaca cgcaggaatg gataggcttg
    21181 gggctggtta cggcgggcgt gttgacgctg gcactgaaac ggtaaagccg caagaaataa
    21241 atgaaatgcc gtctaaaaaa ctgttttcag acggcatttt cgtttctgtc catcctcagc
    21301 actcgaccac gcgcacggat acggggacgg cttttttccg gagcgtggcg gcgagtccgg
    21361 caagcgaggt ttctttgtag gtcgccttca tatcccggcc ggtttgcagc atggttcgga
    21421 tgacttcgtc gagcgagact tttttgtccg tgccgtcttc caaaagcgcg agcgtgccga
    21481 gtttgagggc tttttcggcg gcgatgccgt tgcgttcgat acaggggatt tgcaccagcc
    21541 cgccgacggg gtcgcaagtc agccccaaat ggtgttccat cgccatttca gcggcgtttt
    21601 ccacttgttt gggcgtgccg ccgatgactt cggcgtatgc gcccgccgcc atcgaacacg
    21661 ctacgccgac ttcgccctga cagccgacat ccgcaccgga aatggaggcg ttggttttgt
    21721 agaggatgcc gattgcgccg gcggtgagca ggaagttttc gacgcgctcc tgtgtggcgt
    21781 gggggttgaa cttgcggaaa tagtgcaata cggcgggaat gatgcctgcc gccccgttgg
    21841 tcggggcggt aacgacgcgt ccgccggcgg cgttttcttc gttgaccgcc atggcgtaca
    21901 ccatcggcca gagctgggtg ttgacgattt cggtttcgcg caggactttg agcttggtgg
    21961 caagatgcgg ggcgcggcgg cggacgttca atcagccggg cagttcgccg tccgcaccca
    22021 agccgcgttt gatgcagcct tccataacct cggcaacggc agcggcgcgg cggcggattt
    22081 cggcttcgcc gcatcctgca agcgcggctt cgtttgccaa cacgacttcg gagatgtcga
    22141 gccggttcag acggcatcgg gcaagcagtt cggcgcaact ggtataggga tagggaacgg
    22201 ctttttccgt ttccgcctgc cggtcaaaat cttcttcggt aacgacaaag ccgccgccga
    22261 ccgaataata aacctgttca ttcaataccg tgccgtctga agcataggcg gtaaaacgca
    22321 ggccgttggg gtgtttgggc agcacttgat tgccgagtat gttcaggtcg cggtcgggga
    22381 tgaagcggat ttcttgcccg ttgagccgga ggatgtgctg cgtgcggatg cgttcgaggc
    22441 gttcgggaat gccggcaagc gggatgtcgt gcggcaggct gccttccaaa ccgagcatca
    22501 gcgcgtcaaa tgtaccgtgt ccgtatccgg tcagtgcgag cgagccgtaa atgtcgatga
    22561 cgatgcgaac agcctgtgca tccaaacctg ccgcaaaggc ggcggctgcc ttcatcgggc
    22621 cgaccgtatg cgaactggaa ggcccgatac cgattttgaa aatatagtgg attaacaaaa
    22681 accagtacag cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca
    22741 ccaagtgaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
    22801 tttgttaatc cactatatca aaaatgctga ccatggtttt gctccgaaag gcggtttcag
    22861 acggcatggc ttccgtttga caaaccaaaa ggagacgcgg cacgatgccc gtctcctttt
    22921 ttaaaacggc acttatgcgt cgatattttg ggcaatcagc gcgttgtttt cgataaaggc
    22981 gcggcgcggt tcgacctcgt cgcccatcag ggtaacgaat acttcgtcgg cggcaatggc
    23041 atcttcgatg cgcactttca acaggcggcg cacggcggga tccatcgtgg tttcccacag
    23101 ctgttcgggg ttcatctcgc ccaagccttt gtatcgttgg atggacatac ctttttgggc
    23161 aacgctcatc aagatgtcca aagcggtttc aaagctgtcc gcgtcgtacc cgttttcgcc
    23221 tttgtaaagc ttggcaccct cgccgaccat gcctttaagt gcggcggcgg tttgggtaag
    23281 ggtttgatag gctttgctgt tgaggaactt aggctcgagg tagctcacca ccacattgcc
    23341 gtgcagcttg cgtgtgattt tgatgaactg gtgtccttcg tggccttcga tacgttcgag
    23401 cgcgacttct ttttcgtcca agaggccgct caattcggca acggagcggt ctgcgctttc
    23461 tgctgtgctg agatcgaccg gcgaagcatg tagcatggca tgcaggacga gttcgtcaat
    23521 gatgcggctt tcttgctcga tgacggtttt ggccaacagg aattgtttgg cggtgtcggc
    23581 aagttctgcg ccttcgatgg tgcggccgtc tgaaatgatt ttggcttttt ccaaggcaag
    23641 accgagcagc cattggtctt tttccaactc gtccttgagg taacgttcct gtttgccgta
    23701 tttcgcttta tacaaaggcg gctgggcgat atagatgtag ccgcgctcga ccagttcggg
    23761 catttggcgg tagaagaagg tcaaaagcaa ggtgcggata tgcgcaccgt ccacatcggc
    23821 atcggtcatg ataatgatgc ggtggtaacg cagcttctcg gcattgaatt cttctttgcc
    23881 gatgcccgcg cccaacgcgg taatcaaagc ggctacttcc tgactggcga gcattttttc
    23941 aaagcgcgct ttttcgacgt tcaaaatctt gcccttaagc ggcaaaatcg cttggaattt
    24001 gcggtcgcgc ccctgcatgg cggaaccgcc tgcagaatcg ccctcgacca gataaagttc
    24061 tgacaatgcg gggtcttttt cttggcagtc ggcgagttta ccgggtagcc ccaagccgtc
    24121 catcacgcct ttgcggcggg tgatttcgcg ggctttgcgg gcggcttcgc gcgcgcgggc
    24181 ggcatcgacg attttgccgg tgatgatttt ggcttcggca gggttttctt cgaggaagtc
    24241 ggtcagggct tggctgatga cttcgttgac gacgggaccg atttcgccgg aaaccagttt
    24301 gtctttggtt tgggacgaaa atttggggtc gggcagtttg acggacaaca cgcaggtcaa
    24361 accttcgcgc atatcgtcgc cggcggtttc cactttggct tttttggcga cttcgttggc
    24421 ttcgatgtag ctgttgatgg tgcgcgtcat cacttggcgc aacgcggtca ggtgcgtacc
    24481 gccgtcgcgc tgagggatgt tgttggtgaa gcactgcacg ctttcctgat agctgtcgtt
    24541 ccattgcatt gcgcattcga cgctcatgcc gtctttctcg ccgaacgcgt agaagatttt
    24601 ttcgtgcaag ggcgtttttt tgcggttcat gtattgcacg aatccagcca cgccgccgga
    24661 aagggcgaag ctttcgtgtt tgccgtcgcg ctcgtcggtc aattcgatgt ccacgccgtt
    24721 gttcaggaac gaaagttcgc ggatgcgttt ggcaaggatg tcgaagctgt attcgacgtt
    24781 gccgaaggtt tccgtactgg cgaggaagcg cacggtcgtg ccttttttat cggaatcgcc
    24841 gacaacgttc agcggctctt cggtttcgcc gcgcacgaag cggacgaagt gttctttgcc
    24901 gtcgcggtag atggtcagcg taacccagtc ggacagcgcg ttgacgacgg acacgcccac
    24961 gccgtgcagg ccgccggaga ttttgtagct gttgttgtcg aatttaccgc ccgcgtgcaa
    25021 tacggtcatg atgacttcgg cggcggagcg tccttctttc gggtggatgc cggtgggcat
    25081 accgcgcccg ttgtcggcga cgctgacgga atggtcggcg tgtatcgtta ccgtgatttt
    25141 gtcgcaatgt ccggcgagtg cttcgtcaat ggcgttgtcc aatacttcga acaccatgtg
    25201 gtgcaggccg ctgccgtcct gcgtgtcgcc gatgtacata ccggggcgtt tgcgtaccgc
    25261 ttccaagcct tcgagcacct gaatgctgtc ggcgccgtat tcttcgtgtt tttgttcagt
    25321 catatttttt gccggatttt gaaaagattt tgcgatgccg ccaaaacaag tccgcacctt
    25381 gtagaaaaag cgggcgggac ggcatatata attgtgtatt atagccgatt ttgccgccta
    25441 attcagcgtt atccgcatca gtgtgccgcc gggaaaagat gaaacggtac gtttgcctcc
    25501 ggcatcaggt cggggatttt cccgtaaagt ggcaaaagcg tttttttgcc actaaaatct
    25561 acaccctata cttttcggac aggggcgcgg aaatggaaat atggaatatg ttggacactt
    25621 ggctcggtgc cgtcccgata cgtgcggagg cggtcgaatc cgtggcggcg gttgcggctt
    25681 tgctgctggc gcgcgccctt ctgttgaata tccacttcaa acggcatccg gatttcggca
    25741 tcgaaagcaa gcggcggttt ttggttgcca gccgcaatat aacgctgctt ttggtgctgt
    25801 tttcgctggc atttatctgg tcggcgcaaa tccaaacgct ggctttgtcg atgtttgcgg
    25861 tggcggcggc ggtcgtcgtg gcgacgaagg aactgattat gtgtctgtcg ggcagtattt
    25921 taaggtctgc cacccagcaa tactcggtcg gcgactatat cgaaatcaac ggcctgcgcg
    25981 ggcgcgtggt cgacatcaac ctgttgaaca cgctgatgat gcaggtcggt ccgaacccct
    26041 tggtcggaca gcttgcggga accaccgttt ctttccccaa cagcctgttg ttgagccacc
    26101 ccgtgcgccg cgacaatatt ttgggcgact atgtcatcca tacggtcgaa atccccgttc
    26161 ccatccattt ggattcggat gaagccgtat gccgtctgaa agccgtactc gagcccttgt
    26221 gcgcgcccta catccccgcc atccaacggc atttggaaaa cgtgcaggcg gaaaaactgt
    26281 ttatcacgcc cgccgccaga ccgcgcgtta cccgcgtgcc gtacgatgac aaggcatacc
    26341 gcatcatcgt ccgcttcgcc tcccccgttt caaagcggct ggaaatccaa caggcggtta
    26401 tggacgaatt tttgcgcgta caataccgcc tgttaaatta ccccgccggc tccgaaacac
    26461 tttaactttc cctgaccgac cccatttccg gcttcagacg gcatattgcc gatatgctgt
    26521 ctgaagcaca acacgcaaag gaaacccatc ttatgactga caacgcactg ctccatttgg
    26581 gcgaagaacc ccgttttgat caaatcaaaa ccgaagacat caaacccgcc ctgcaaaccg
    26641 ccattgccga agcgcgcgaa caaatcgccg ccatcaaagc ccaaacgcac accggctggg
    26701 caaacactgt cgaacccctg accggcatca ccgaacgcgt cggcaggatt tggggcgtgg
    26761 tgtcgcacct caactccgtc gccgacacgc ccgaactgcg cgccgtctat aacgaactga
    26821 tgcccgaaat caccgtcttc ttcaccgaaa tcggacaaga catcgagctg tacaaccgct
    26881 tcaaaaccat caaaaactcc cccgagttcg acaccctctc ccccgcgcaa aaaaccaaac
    26941 tcaaccacga tctgcgcgat ttcgtcctca gcggcgcgga actgccgccc gaacagcagg
    27001 cagaactggc aaaactgcaa accgaaggcg cgcaactttc cgccaaattc tcccaaaacg
    27061 tcctagacgc gaccgacgcg ttcggcattt actttgacga tgccgcaccg cttgccggca
    27121 ttcccgaaga cgcgctcgcc atgtttgccg ctgccgcgca aagcgaaggc aaaacaggct
    27181 acaaaatcgg tttgcagatt ccgcactacc tcgccgtcat ccaatacgcc gacaaccgcg
    27241 aactgcgcga acaaatctac cgcgcctacg ttacccgcgc cagcgaactt tcagacggcg
    27301 gcaaattcga caacaccgcc aacatcgacc gcacgctcgc aaacgccctg caaaccgcca
    27361 aactgctcgg cttcaaaaac tacgccgaat tgtcgctggc aaccaaaatg gcggacacgc
    27421 ccgaacaagt tttaaacttc ctgcacgacc tcgcccgccg cgccaaaccc tacgccgaaa
    27481 aagacctcgc cgaagtcaaa gccttcgccc gcgaaagcct gaacctcgcc gatttgcaac
    27541 cgtgggactt gggctacgcc agcgaaaaac tgcgcgaagc caaatacgcg ttcagcgaaa
    27601 ccgaagtcaa aaaatacttc cccgtcggca aagtattaaa cggactgttc gcccaaatca
    27661 aaaaactcta cggcatcgga tttaccgaaa aaaccgtccc cgtctggcac aaagacgtgc
    27721 gctattttga attgcaacaa aacggcgaaa ccataggcgg cgtttatatg gatttgtacg
    27781 cacgcgaagg caaacgcggc ggcgcgtgga tgaacgacta caaaggtcgc cgccgttttt
    27841 cagacggcac gctgcaactg cccaccgcct acctcgtctg caacttcgcc ccgcccgtcg
    27901 gcggcagaga agcgcgtttg agccacgacg aaatcctcat cctcttccac gaaaccggac
    27961 acgggctgca ccacctgctt acccaagtgg acgaactggg cgtatccggc atcaacggcg
    28021 tagaatggga cgcagtcgaa ctgcccagcc agtttatgga aaatttcgtc tgggaatacg
    28081 acgtcttggc acaaatgtcc gcccacgaag aaaccggcgt tcccctgccg aaagaactct
    28141 tcgacaaaat gctcgccgcc aaaaacttcc aacgcggcat gttcctcgtc cggcaaatgg
    28201 agttcgccct ctttgatatg atgatttaca gcgaagacga cgaaggccgt ctgaaaaact
    28261 ggcaacaggt tttagacagc gtgcgcaaag aagtcgccgt catccaaccg cccgaataca
    28321 accgcttcgc cttgagcttc ggccacatct tcgcaggcgg ctattccgca ggctattaca
    28381 gctacgcgtg ggcggaagta ttgagcgcgg acgcatacgc cgcctttgaa gaaagcgacg
    28441 atgtcgccgc cacaggcaaa cgcttttggc aggaaatcct cgccgtcggc ggatcgcgca
    28501 gcgcggcaga atccttcaaa gccttccgcg gacgcgaacc gcgcatagac gcactcttgc
    28561 gccacagcgg tttcgacaac gcggcttgat cgatgtgaac aaaaaatgcc gtctgaaaca
    28621 atcaaacagt gtttcagacg gcatcagcat ttgcgcggtt tcgccatttt cacaaaaaca
    28681 gtaagccaaa atcccgtcat tcccgcgtag gcgggaatcc agaccggtcg gcacggaaac
    28741 ttatcgggta aaaaggtttc tccggtcctg aatcctagat tcccgctttc gcgggaatga
    28801 cggaatattt ttgaatttga taaaaatgcc gtctgaaacg gtcaaacaac gcttcagacg
    28861 gcattttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa
    28921 tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcca
    28981 aggcgaggca acgacgtact ggtttttgtt aatccactat attttccgac atcattgaat
    29041 caaacccaaa tgcgacaaga gcgtccatgt gccgatggca atcaacacca aacctccggc
    29101 aaattccgca cacctgccga acaatacgcc caaagccctt cccgccgtca gcccgaccgt
    29161 aaccatcacc gtcgtcgcca tgccgatgac ggcggcggca aaggcgatgt ttacctccat
    29221 aaacgccaag cccaccccga ctatcatgga atcaatactg gttccaaaag cagtcagaac
    29281 cgtcaaccac cagctttccc gtttggcttc gcgcacatcg tccgccttgc cggacagccc
    29341 ttcgcgcatc attttcagac ccagcccgcc cagcaggacg aaagccgccc aatggtccca
    29401 ttcgctgata aacggtttgg cataaaaacc gcctacccag cctgccagcg gcgtgagtgc
    29461 ttcaaccgag ccgaacacca aggctgttgc tgcaattttg cgcggaggca tcctgaccgc
    29521 cgcacccttt gccaatgcga cggcaaacgc atccatcgac atccccagcg caatcaagag
    29581 caaagcgtaa agactcatac cgcacccatc ctcaaaaagg gcagattata gcaaaacagc
    29641 aaagcgcgaa aaacgccgca cgaaaaccca catcccgtca ttcctgcaaa aacaaaaaat
    29701 caaaaacaga aatcccgtca ttcccgcgca ggcgggaatc cagaccggtc ggtgcagaaa
    29761 cttattggat aaaacggttt ctttagattt tacgttctag attcccactt tcgtgggaat
    29821 gacgggatgt aggtttccgt gcggatggat tcgt
//
LOCUS       DAWFJW010000014        28208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFJW010000014 DAWFJW010000000
VERSION     DAWFJW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.014"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGR19_000507"
     CDS             complement(181..2109)
                     /gene="dnaK"
                     /locus_tag="WGR19_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0798093.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2317..2589)
                     /locus_tag="WGR19_000508"
     CDS             complement(2317..2589)
                     /locus_tag="WGR19_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798094.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2775..3461
                     /locus_tag="WGR19_000509"
     CDS             2775..3461
                     /locus_tag="WGR19_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0798095.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3593..3931
                     /gene="erpA"
                     /locus_tag="WGR19_000510"
     CDS             3593..3931
                     /gene="erpA"
                     /locus_tag="WGR19_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0798096.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3948..4094
                     /locus_tag="WGR19_000511"
     CDS             3948..4094
                     /locus_tag="WGR19_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798097.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4091..4555
                     /locus_tag="WGR19_000512"
     CDS             4091..4555
                     /locus_tag="WGR19_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0798098.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            4590..6101
                     /gene="ubiB"
                     /locus_tag="WGR19_000513"
     CDS             4590..6101
                     /gene="ubiB"
                     /locus_tag="WGR19_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0798099.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWINGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGR19_000514"
     CDS             complement(6458..7276)
                     /gene="cysE"
                     /locus_tag="WGR19_000514"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0798100.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            7460..8038
                     /gene="grpE"
                     /locus_tag="WGR19_000515"
     CDS             7460..8038
                     /gene="grpE"
                     /locus_tag="WGR19_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0798101.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGR19_000516"
     CDS             complement(8162..8377)
                     /gene="nqrM"
                     /locus_tag="WGR19_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0798102.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8403..9458)
                     /locus_tag="WGR19_000517"
     CDS             complement(8403..9458)
                     /locus_tag="WGR19_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0798103.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISWFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGR19_000518"
     CDS             complement(9611..10828)
                     /gene="nqrF"
                     /locus_tag="WGR19_000518"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0798104.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMEIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGR19_000519"
     CDS             complement(10842..11435)
                     /gene="nqrE"
                     /locus_tag="WGR19_000519"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0798105.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(11439..12065)
                     /locus_tag="WGR19_000520"
     CDS             complement(11439..12065)
                     /locus_tag="WGR19_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0798106.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(12065..12841)
                     /locus_tag="WGR19_000521"
     CDS             complement(12065..12841)
                     /locus_tag="WGR19_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0798107.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARVAAKDPA
                     QSIQIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(12834..14066)
                     /locus_tag="WGR19_000522"
     CDS             complement(12834..14066)
                     /locus_tag="WGR19_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0798108.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGVQAFGALTPDLLQQNIANDWHYTLAD
                     ALGINMSSEASVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(14069..15412)
                     /locus_tag="WGR19_000523"
     CDS             complement(14069..15412)
                     /locus_tag="WGR19_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0798109.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(15810..17231)
                     /locus_tag="WGR19_000524"
     CDS             complement(15810..17231)
                     /locus_tag="WGR19_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0798110.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(17589..17948)
                     /locus_tag="WGR19_000525"
     CDS             complement(17589..17948)
                     /locus_tag="WGR19_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0798111.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(17945..18550)
                     /locus_tag="WGR19_000526"
     CDS             complement(17945..18550)
                     /locus_tag="WGR19_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0798112.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGILQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(18597..19079)
                     /locus_tag="WGR19_000527"
     CDS             complement(18597..19079)
                     /locus_tag="WGR19_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0798113.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      19283..19380
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19591..20691
                     /gene="gcvT"
                     /locus_tag="WGR19_000528"
     CDS             19591..20691
                     /gene="gcvT"
                     /locus_tag="WGR19_000528"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0798114.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            20853..21239
                     /gene="gcvH"
                     /locus_tag="WGR19_000529"
     CDS             20853..21239
                     /gene="gcvH"
                     /locus_tag="WGR19_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0798115.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNDDLPSAPETANSDPYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            21391..22638
                     /locus_tag="WGR19_000530"
     CDS             21391..22638
                     /locus_tag="WGR19_000530"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0798116.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            22914..23339
                     /locus_tag="WGR19_000531"
                     /pseudo
     CDS             22914..23339
                     /locus_tag="WGR19_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            23345..23923
                     /locus_tag="WGR19_000532"
                     /pseudo
     CDS             23345..23923
                     /locus_tag="WGR19_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003759061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
     gene            24107..24760
                     /locus_tag="WGR19_000533"
                     /pseudo
     CDS             24107..24760
                     /locus_tag="WGR19_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712464.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            24818..25438
                     /locus_tag="WGR19_000534"
     CDS             24818..25438
                     /locus_tag="WGR19_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0798117.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            25449..25580
                     /locus_tag="WGR19_000535"
                     /pseudo
     CDS             25449..25580
                     /locus_tag="WGR19_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            25577..26071
                     /locus_tag="WGR19_000536"
     CDS             25577..26071
                     /locus_tag="WGR19_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0798118.1"
                     /translation="MKKTLLAIIAVFALSACRQAEEGPPPLPQQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVSFDDMPDT
                     YIFK"
     gene            complement(26128..26232)
                     /locus_tag="WGR19_000537"
                     /pseudo
     CDS             complement(26128..26232)
                     /locus_tag="WGR19_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(26310..27971)
                     /locus_tag="WGR19_000538"
     CDS             complement(26310..27971)
                     /locus_tag="WGR19_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0798119.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIAYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMINVNYKEYASPETRYCPAGVYEIIE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         6716 a         6871 c         7634 g         6987 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatataa aaaatttcca gagaaccgat acaacagttg
       61 gaacttgggt ttgggaatat tacggtagat gaacttggaa cctctgttat gctatggtct
      121 tttatctcaa ttgaaaaaag cgcgaatcaa acggttcgcg cttttttcag acggcatcaa
      181 ttattttttg tcgtctttta cttcttcaaa gtcggcatct acgacatcat cgtctttctt
      241 tgcagaagca ttggcttgtt cgctttcgcc tgcttgggct tcagcttgtg cttgagcgta
      301 aaccatttcg cccagttttt ggctggctgt gcccagcgcc tcggctttgg catcgatggc
      361 ggctttgtcg tcgcctttca cggcttcttc ggcttctttc agcgcggctt cgattttttc
      421 tttttcggca gcgtcgagtt tgtcgccgta gtccgccaaa gattttttca cggagtgaat
      481 cagggcttcg gcttggttgc gggaagcgac caattcagtc agctttttat cttcctcagc
      541 attggcttcg gcatctttca ccatgcgttc gatttcttct tcgctcaaac ctgaagaacc
      601 ttggatggta atgttggctg ctttaccggt gcctttgtct ttggcggaaa cgtgcaggat
      661 gccgttggcg tcgatgtcga aggttacttc gatttgcggc ataccgcgcg gtgcaggtgc
      721 gatgtcgccc aagttgaact gacccaaaga tttgttggca gaagcgcgtt cgcgttcgcc
      781 ttgcagtacg tggatggtta ctgcgctttg gttgtcttcg gcggtagaga acacttgcga
      841 cgctttagtc gggatagttg tgttcttttg aatcagcttg gtcatcacgc cgcccatggt
      901 ttcgataccc aaggacagag gggttacgtc cagcagcaat acgtcgctgc ggccgccgct
      961 taacacttcg ccttgaatcg ctgcgcctac ggcgacagct tcgtcagggt tcacgtcttt
     1021 gcgcggctct ttgccgaaga agtctttaac ggcttcttgt actttcggca tacggctttg
     1081 accaccgacc aaaatcacgt cgtcgatgtc gccagtgctc agaccggcat ctttcaatgc
     1141 ggtgcggcaa ggctcgatag agcgggcaat caggtcttca accaggcttt cgaatttggc
     1201 gcgggtaatt ttcatcgcca agtgtttcgg gccggttgcg tccatggtga tgtacggcag
     1261 gttgatttcg gtttgctggc cgctggacaa ttcgattttg gctttttcgg cagcttcttt
     1321 cagacgttgc agagccatga cgtcttgttt caaatcaatg ccttgttctt ttttaaactc
     1381 gtcaatgatg tagtcgatca agcgttggtc gaagtcttca ccgcccaaga aagtatcgcc
     1441 gttggtagcc aatacttcaa attgtttgtc gccgtcgagg ttggcgattt cgatgatgga
     1501 aatatcgaaa gtaccgccgc ccaagtcata tacggctact ttgcggtctt tgttgtcgcc
     1561 tttgtccata ccgaatgcca aagcggctgc ggtcggctcg ttgatgatgc gtttcacgtc
     1621 caaaccggcg atacggcctg cgtctttggt agcttgacgt tggctgtcgt tgaagtaggc
     1681 cggaacggta atcacggctt cggttacttt ttcgcccaag taagcttcgg cggcttcttt
     1741 cattttacgc aggacttctg cggaaatttg aggaggagac agctctttgc cttgcgcttt
     1801 cacccatgcg tcgccgttgt tggctttgat gatttcaaaa ggcatagatt cgatgtcgcg
     1861 ttggacttct ttgtcttcaa atttgtggcc gatcaaacgt ttggcggcgt aaatagtgtt
     1921 tttagcgttg gttacggctt ggcgtttggc aggcgcgccg acgaggattt cgccgccgtc
     1981 caaataagcg ataacggacg gcgtggtgcg tgcgccttct gcgttttcga taactttggt
     2041 ttgaccgttt tcggaaatgg ccaaacaaga gttggttgta cctaagtcga taccgattac
     2101 ttttgccatg tggataatcc tatttgattt tgcttatttt gagaaatatg ttggaacatt
     2161 ttgtcccgat gggctgtaaa tagggcgggc ggcgggctgt ttcaagctac agcatggcta
     2221 taagtatata actttatgaa tatattggtt ttatatttga tttaatacat ttggctccaa
     2281 tgcattcaag cataatgttt caaatggcag gcaggtttat tcatagacga tgccggcgag
     2341 catttcctgt tcgttcaagt tgccgtactc tttttcccag tcgtgtgaag actcgatgat
     2401 gtcgcattct ttggaaaggg agacttgttc tgcatccata tctttggcgt tcagtatgtt
     2461 gaattgttcg cacagggatg cggataaagt gatgtcaggc tgtttggctt cagaacggtt
     2521 ttcttggaag gcaaagcaga atgcggtaaa tgccgcagca tagataagat atttgccggt
     2581 tttcttcatt ttctatcctt tttctgtcaa ttcaggatta aacctatgga aaatcttaaa
     2641 aattatgtat ttagtaagaa aaatcataat ttaaatttag tttatcatag ttgttccgtt
     2701 tttgggatag ctaaggtaaa atatatttca tgtttacttt agatgattga atgaagggga
     2761 gcggaaggat atttatggaa acctttaaag acagactggt ttttttatgg aaaagcgaag
     2821 cgaagcaggc aaaaatcgca tccgatattg aaatgacgat tgcgggcttc agcaggatat
     2881 ggaatgaagg cggtctgccg aagtctgaaa cattgaaaaa aatcaagcag ttgaaggggt
     2941 gtagtatcga ttggctgctg accggggagg gtaatccgtt tccggatgaa gccccaaaaa
     3001 aatcccttgc ttacgatacc ttgggcaatg aagtcgatac ggacgagttt gtctttgtgc
     3061 cgagatatga tattcgggcg gctgcgggat acgggcagtt tgtcgatcat gaggaaccgg
     3121 tatttacaat ggcgttcaga cggcattgga ttgagaatta tgttacccgc gatacgaaaa
     3181 acctgtctgt aatttccgtc aagggggatt cgatggaggg ggttttgaat gacggcgatt
     3241 cgattttggt caatcatggt gaaaatacgc cgagggacgg tctgtatgtg ttgcggatta
     3301 atgaaaatct gctggttaaa cgtttacaga ttgtaccggg cgggattatc aatgtgattt
     3361 ctgcaaacga ggcttatcct gcttttgaaa tcaatttgaa cgatttgacc gatgatgtgg
     3421 agattatcgg gcgtgtcgag tggttcggca ggacgatttg agtttggggc ttgaaattgc
     3481 aggcggtcaa acttatctat tggaacaatt cctttttcaa aggcgaagcc tgcttgcctt
     3541 taaagggggt ttgagagaga atgcagaaaa tattatatta aggaataaca ccatgtcgga
     3601 tgaaagccct attattttta ctgacagctg ctgtgccaaa gttgccgatt tgattgccga
     3661 agaaaacaat cccgatttga aattgcgggt ttttgtcaat ggcggcggct gttcgggttt
     3721 ccagtacgga tttacttttg acgaaatcaa aaacgacgac gattttgaaa ttgagaaaaa
     3781 cggtttggtc tttttggtcg atccgatgag ctatcaatat ctggtcggtg cggaaatcga
     3841 ctatacggaa agtttgcagg gttcgcaatt cgtcatccgc aatccgaatg cggaaacaac
     3901 ctgcggttgc ggatcgtcgt tttccgtatg accgcttggt ttgtgtgatg ccgtctgaac
     3961 gttcagacgg catttttact tttggaaaat atattatcgg gatgaattca catataatcc
     4021 gattgtttga agatgaatcg ggtttcccga aaggaacggg cggaacggta tcaggcgtat
     4081 ttgttccctt atgattgaga tgagtaaaga ttaccgaaat gatttgtacg atgtatatgt
     4141 ttcctacccg ccccaagtgg atcgcgggct tatccgggag tgcctcaggg agaatctcgg
     4201 cgaggaaaag gcggaaggat tgatcgaatc gctcgattcc aaacctcaag tgctggttga
     4261 ggaaaaatgc acttgggcga aacgggaaga gttgcatgat tatttcagct atttgggttt
     4321 ggatattatt acccggagat atatggagtt ggaaacggtc gtgccgccgg aggaagggga
     4381 gggcgaagga gaaggcgcgg atggggaaat gcccgaatat cttgaacttc acggcgggcg
     4441 ggaagatgat atttccgcac cttcgcaacc cgaaccgccg tcccgcaata tcaaactgct
     4501 ggttttcggg ctgctgattg cctttttggg ctatctgctc ggtaagattt tttgattgtc
     4561 cgataaatgc tgtattcggg attttatata tgaaatggtt gaaacgcctg acggttattg
     4621 tcgggacttt ttaccgctat cggctggcag gtctgtgtgc ttcgctgatg ggtagcggtt
     4681 ggatatgcgc tctgctgaaa atgatgccgc agtcgtccaa attgaaaaac gaaccgcctg
     4741 ctgtccgtct gcgccttgcc ttggaaagcc tggggccgat tttcatcaag ttcgggcagg
     4801 ttttgtccac acgccccgat ttgattccgc acgattacgc cgtcgaactg gcaaagctgc
     4861 aagacaaagt gccgcctttt gacgcgcggc tttcgcgtga acaaatcgaa aaatcgttgg
     4921 gtcagtccat cgaaaagctg tatgcggaat ttgaaaccga gcccatcgcc agcgcgtcca
     4981 tcgcccaagt acacaaagcc cgcctgcatt cgggcgaacg agtggcggtt aaagttttgc
     5041 gccccaacct tttgcccctt atcgaacagg atttgtcgct gatgcgtttc ggcgcggcct
     5101 gggtcgaacg cttgttttca gacggcaggc gtctgaagcc gctcgaagtg gtggcggagt
     5161 tcgacaaata cctgcacgac gagttggatt tgatgcgcga agccgccaat gccggacagc
     5221 tcgggcgcaa tttccaaaac agcaatatgc tgattgtgcc caaggtgttt tacgactact
     5281 gcaccagcga cgtgctgaca atcgaatgga tggacggcac gccggttgcc gacatcgcca
     5341 aactcaaagc agacggcatc gatttgcaca aactcgccga ttacggcgtg gaaatctttt
     5401 tcacgcaggt cttccgcgac ggctttttcc acgcggatat gcaccccggc aatattttgg
     5461 ttgccgccga caaccgctac atcgccctcg atttcggcat cgtcggcacg ctgaccgatt
     5521 acgacaaacg ttatctcgcc atcaatttcc tcgccttttt caaccgcgat taccggcgcg
     5581 tcgccaccgc ccacatcgaa tcgggctggg tgcctgccga cacgcgtgcg gaagaattgg
     5641 aagccgccgt ccgcgccgtg tgcgaaccgg tgttcaacaa accgatttcg cagatttcct
     5701 tcggcttggt gctgatgcgc ctgtttgaag tcagccgccg cttcaatgtc gaaatccaac
     5761 cgcagcttgt gctgctgcaa aaaacgctgc tcaacatcga aggcttgggc cgccagctcg
     5821 atcccgattt ggacttgtgg aaaaccgcca aaccgttttt ggtgaaatgg ataaacgggc
     5881 aggtcggccc taaagccctt tggcgcaacc tcaaaaacga agcccccgac tgggcgcaaa
     5941 tcatcccttc attgccgcgc aaaatcagtg cgttgattga tgaaaaccgc cagcaggaaa
     6001 tgcgtgatgc ctatatccat ttggtcaaag tgcagcagcg gcaaagcctg tggctggctg
     6061 tgattgcggt tgttttgctg ctgattttgc ttttgaaata ggctttgtcc gaatcatcgc
     6121 ccgactccgc ccgtttataa ggaaatcggt tatagtggat taacaaaaac cagtacggcg
     6181 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
     6241 attccgtact atccgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
     6301 ctatatttcc ggttgcgtgg gaatcgggtg tattgaataa aaggcatttt gtccgactgg
     6361 caagtgccga catcggcggc atatcaaggc gcaggcttga agcgggcaat gtcgtctgaa
     6421 gcccgtttgg cgtttcagac ggcattggtg cggatattca aatcataaag tcgatttcgg
     6481 taaactggat attttgatcc atatccgccg acggtgtttt gagcgatcgc gccacgggtt
     6541 tggcgggtac gccgacaacc gtgatggacg gcggcacgtc tgaaaccacg acgctgcccg
     6601 ccccgatttt ggcattgctg ccgatgcgga tattgcccaa tatcgaggcg tttgcgccga
     6661 tcatcacgcc gtcgccgatt ttagggtggc ggtcgccgcc ttctttgccc gaaccgccga
     6721 gcgttacgcc gtgcaaaatc gaaatattgt tgcccaacac ggcggtttcg ccggcaacaa
     6781 agccggtggc gtggtcgagc atcagcccgt atccgaaacg ggcggcggga tggatgtcca
     6841 cgccgaatac ttcggacata cggttttgca ggaaatacgc cagcgttttg cgcccgtcga
     6901 gatacagccg atggttgatg cggtgtgcct gaatcgcgtg gaagcctttg aaatataaaa
     6961 gcggcagcga atattcgtcg caggcgggat cgcgttcgta gatggctttt aagtctgctt
     7021 cgacgcattt gccgatttgg gtgtcgctgc ccaacgcctg ctggtagatt tcaaacagcg
     7081 cgcgcacgtc cataatcggg ctgccgagtt tgctggaaag gtggtaggca aggacggagc
     7141 cgagggactc gtggcgcaac acggtttggt gcaaaaaact tgccagcatc ggttcggcgg
     7201 agaccgcggc cgcggtttct tcgcggatgg tgtgccagag gtcgaaaccg gttgtgttta
     7261 aatggtcttt tttcatgagt gatgacgttt gaaaatcgat atggtcggca gtatcttacc
     7321 gtctatatta ttttttcggt aggggatttg aaaatgaatt tgaaattctc tgcttttgct
     7381 tgaagtttct tgaaaatgtc cttatcttgc gcgggtaata actggatttt gatttccaat
     7441 ttgttttaag ggatacgata tgagcgaaca gacacagcag caaaacagtg aagaagcggt
     7501 tgaaaatgtg gaggcggtgg aaaccgtcga gacagtagga aatgcggacg gtgtgcagga
     7561 acaggctgcc gcagagccgg cttatgagga tttgcaggcg cggattgccg agctggaagc
     7621 gcagttgaaa gacgagcagc tgcgcgcttt ggcaaacgag caaaacctgc gccgccgcca
     7681 ccagcaggaa attgcggata cgcacaagtt cgccggacag aagtttgccg tggaaatgct
     7741 gccggtcaag gattatctgg aaatggcgct tttggatcag agcggcaatt tcgatgcgct
     7801 gaaaatgggc gtgcagatga ctttgaacga gttgcagaaa gcatttgatg ctacgcaaat
     7861 caaggaaatc aatcctaaag cgggcgataa gctcgatccg aatatccatc aggcgatgca
     7921 ggcggtggca agcgaacagg aacccaatac cgtggtgggc gtgatgaaga agggttatac
     7981 gctgtccgac cgcgtgttgc gcccggctat ggttacggtg gcacagaagg aagcctgaag
     8041 gcgtctgggg aataatctga tttatttcct gaagcgcgtt ttgcgtataa accgatcgaa
     8101 gtaaagcggc aatgccgtct gaacccgcct gtcgggcttc agacggcatt ttgtttcggc
     8161 gttaacggtc aacccgtatg ccgcctgcct gtttttcttc atccagtttc ttttgcaggg
     8221 tgtcgcaagg tgtgtcgcag tcgcacattt ttttcatacc caaggcagta atgccgccgc
     8281 aactgccttt gatgctgcgt ttggagaaaa tatagccgac cgccataccg atgatgacgg
     8341 tcaggaagat gccgaaggta aggagcaggg ttttcatggt gtttcctaat cggtttgtat
     8401 gtttagcgga gcagtttttc aaattcggaa gacatggcgg tgcggtagcc gcctttatcc
     8461 ctgacaatca ggaaaacagc gagtttttcg cgctctgcca gctttaaggc ttcggtttcg
     8521 cccaatacga ataatcctgt cgacaagccg tccgccgtca tcgcactgtc tgcgaccacg
     8581 ctgatggagg cgaggttgtg gctgatgggt cgtttgttgt tcgggttgat gatatgggag
     8641 aggcgtttgc cgtttttatc gacgtggaaa atacggtaat cgccggaagt ggcaagcgaa
     8701 cggttgttca gcgggacgat aatctgcgta ttgccgcctt ggacgatatt gggctgctcg
     8761 ataccgatgc gccacggttc gccgcgcgcg tttttgcctt tgccgtgcaa ctcgccgccg
     8821 atttcgacca gataattttg aatgccgtat ttttccagtt cgcccgcaac tttatcaacg
     8881 ccgaagcctt tggcaatcga agataaatcc aaataggcct tggggtgggt tttgctcaag
     8941 gaagcgtaat ctttgccttg tttcaaaatg attttgtcta tgcccgtata agatgccgcc
     9001 tgtttgattt gttccggcga cggttcacgg gtaacggatt tgtcggggcc gaatccccaa
     9061 aggttgacca aggggccgac ggttacgtcc agcgcgccgt gtgtcaggcg gttcaggcgg
     9121 acggcttcgg cagtaacgtg tgcgaagtcg cttgaaatgc ggaggggctt gccggctgtg
     9181 tgttggttga accagctgat ttcggagtcg ggctgatagg tggacatctg ccggttgact
     9241 tctttaagcg cgtcatcgat gcgtttttgt atttcggcag gtgaggggag tttgtcccga
     9301 ttatttgaaa ggtatttgac ggtataggtc gtgcccatcg tttcgccttg cagggtaacg
     9361 gtttgcgcgg tttgttccga acaggcgttc aggaagatga aacccagggc aaatatcaag
     9421 acgcggataa agttcggcag gcgtgtttca gacggcatag tgtttgacgg ttttggcaaa
     9481 tggtttgaat tatatcgcaa aacggccggt atgtttctat gccgatgccg tctgaagggt
     9541 gttcggatgg catcggcata gaaaaaggaa gaaaccgagg tttcttcctt ttgtgtttga
     9601 agccgaatat ttaaccgccg aaatcgtcca agaggatgtt ttcgtcttcc acgcctaagt
     9661 ctttaagcat tttgatgacg gactggttca tgatcggagg accacacata taaaattcgc
     9721 agtcttccgg cgcttcgtgg tttttcagat ggttttcgta aaccacgttg tgaatgaagc
     9781 ctgtgtagcc gtcccagttg tcttcaggca atgggtcgga cagggcgacg tgccacgtga
     9841 agttcgggaa ctctgccgcg agttggtcaa agtcttcgac atagaacatc tcgcgtttgg
     9901 aacgtgcgcc gtaccagaag gtaatcttac gtttggagtt caaacgtttc aactggtcga
     9961 aaatgtggga acgcatcgga gccatacccg caccgccgcc gataaatacc atttcggcat
    10021 cggtgtcttt ggcgaaaaat tcgccgaacg ggccggaaat cgtaactttg tcgccgggtt
    10081 tgagcgacca gatgtaggac gacatttgtc ccggaggcgc atcaggtacg cgcgggggcg
    10141 gcgtggcgat acgcacgttc agcataatga tgcctttttc ttcaggatac gaagccatag
    10201 agtaggcgcg caaaatcggc tcgtccactt tggaaacgta ttgccacaaa ttgtatttgt
    10261 cccagtcttc gtgatattcc ttaggaatgt cgaagtcttt gtaggcaaca gtgtgcggcg
    10321 gggcttcaat ttgaatgtag ccgccggcgc ggaaggggac ttcttcgcct tcgggaatgg
    10381 caagcttgag ttctttaatg aacgtggctt tgttgtcgtt ggaaatgacg gtgcattccc
    10441 attttttcac gccgaacact tcttcgggga cttcaatctc catatcggtt ttgacgttga
    10501 cttggcacga cagccggcag ccttcgcgtg cttcgcgttt gctgatgtgg gacagctcgg
    10561 tcggcaggat gtcgccgccg ccgcttttta cgacgacgcg gcattgtccg cacgaaccgc
    10621 ccccgccgca ggcggagggg ataaagatgc cttcgttggc aagcgcgccc aagagtttgc
    10681 cgccggcggg catcgtcagc tctttttcgc cgttgacttt gatggtgatg tcgccttcgc
    10741 tgaccagttt ggatttggca aacagaatca tcagtgccaa aaccaaaacg atgacggtaa
    10801 acatcacgat acctaaaata atctccatac cgatcccttt cttataactg gatgccagag
    10861 aacgacataa acgccatcgc catcaggccg gcggcgataa aggtaatgcc caagcctttg
    10921 aggcctttgg gagcgtccga atatttcatt ttttcggtaa tgcccgccaa agcgacaatc
    10981 gccaacatcc agcccaagcc cgcgccgaag ccgtatacaa cggactcgcc gaagttgtat
    11041 tcgcgttgcg ccataaacga tacggcgccg aaaatcgcgc agttcacggt aatcagcggc
    11101 aggtagatgc ccaatgcgtt atagagggcg gggacgaatt tatccaagaa catttccaaa
    11161 atctgcacca aagcggcaat cacgccgatg aaggtgatga atttcaaaaa ggtcaaatcc
    11221 acgccttcga caatcgcgcc gtctttgagc agcgagtaaa cgagttggtt gacagggacg
    11281 gacagcccga gtacgaaaat taccgccaca cccaaaccga atgcggtgga tacttttttg
    11341 gataccgcca aaaacgtgca catacccaaa aagaaggata gtgccatatt ttcaatgaag
    11401 acggatttga tgaagaggct caaatagtgt tccatagctt attcctccgc ctgttcgggt
    11461 ttccaggtac gcagtcccca aatcaaaaag ccgatgatga agaacgcgct gggggcgagc
    11521 aggaacaagc cgttggtctg ataccagccg ccgtcctgca cggtttggaa aacggtgtag
    11581 cccaagagtt tgcccgagcc aatcagttcg cggacggtgg cgacgacaag cagcattatc
    11641 ccgtagcccg cgccgttgcc gatgccgtcg atcaggcttt ccagcggcgg ctctttcatc
    11701 gcaaatgctt cggcgcggcc catcacgata cagttggtaa taatcagacc gacgaatacg
    11761 gaaagctgtt tggacaattc gtaggcaaat gcctgcaaga gttggtcgac cagcgtaacc
    11821 agcgacgcga taatcgccat ttgcacgata atacggatgc tgttggggat gtagttgcgt
    11881 accagcgaaa tgaagaagct ggaaaaaccg gttaccaaag ctacggaaat acccatcacg
    11941 atggccgtct gaagtttggt ggtaaccgcc aaagccgaac aaatacccaa aacctgcaag
    12001 gcaatcgggt tgttgtcgat aaagggtgaa aacatcaaat gtttcaagcg tttcatatca
    12061 gccattattg cgctcctgct gatttcaatt tgttcaggta ggggatatag ccgttttcgc
    12121 cgaaccagta ggcgaacgaa ccttgcacgc ctttggatgt cagcgatgcg ccggagaggg
    12181 catctacgcc gtgttctttg tccgaacccg cgcctttgcc gacgtgcagg gcgagtttgc
    12241 cttgtccgtc aaacagtttt ttgccgacga atttttgctg ccacaacgga ttgccgattt
    12301 cgccgcccaa gcccggggtt tcgccttgtt cgtagtaggt aatgccgttg atggtgttgc
    12361 cgtcgggctg gatggcgaca aagccgtaca tgaccgacca caaaccgttg ccgtgcatag
    12421 gcaggatgat ttgcccgatt ttgccgtctt caccttttac caaataaacc tcagtgtatt
    12481 tggcacggct tttgatgcct gccaaatcgt cttcgggttt gatttggatg ctttgcgcgg
    12541 gatctttggc agcaacacgc gcgctgaagt ctttcggcgc gtctttcaca tattcgccgg
    12601 tcgccaaatc gacaacacgt tgctcgatac gctcggcaaa ggttttaccg atgtcggtgt
    12661 ccttatccat caaaccggct acgctcaaga tatagccttg tttgtcttgg agtttttgtt
    12721 tctcttggat gggtttcaag ccgacgaccg caccggcaac gatgaccgag caaatcaggc
    12781 tgaccgccaa cacgacaatc agcgtgccgc tgaagctgtc tttatcgaat ttcttagcca
    12841 ttgctgcgcg cctttctgcg tttgatgttc gcttgtgcga cgaaatagtc gaaaatcggg
    12901 gcaaacaggt tggcaaacag aatcgccaac atcatgcctt cggggtaagc cggattgacc
    12961 acgcggatta acacgcacat cacaccgatc agcgcaccgt accaccattt gccgacattg
    13021 gtaaaggaag cggaaacagg gtcggtcgcc ataaacagca taccgatggc gaagccgccg
    13081 acgaccaaat gccagtacca aggcatagca aacatggcgt tggtgtccga accgatgaag
    13141 ttgaacagcg aagacatcgc aatcataccg atcatcacgc cggcaataat gcgccaagaa
    13201 gcgatgcggg caaacacgat aaacgcgccg ccgattaaga gtgccaaagt ggagacttcg
    13261 ccgatggagc cgggcagttt gccgataaac gcgtccatcc aagtgatggt ttgaccggtt
    13321 acggcgtttt tcaggccgtc tgcaccgtgt gccgcccatt gcgccagcgc ggttgcgccg
    13381 gaatagccgt caaccgccgt ccaaaccgca tcgccgctca agttggcggg gtaggcgaag
    13441 aacaggaagg cgcggcctgc cagcgcaggg ttcataaagt ttttacccgt accgccgaat
    13501 acttctttcg caaccacaac gccgaaagaa atacccaaag ccgcctgcca cagcggcagc
    13561 gtgggcggaa cgattaaggc aaacagaatc gaggtaacaa agaaaccttc gttgatttcg
    13621 tgtttgcgca cggtggcgaa caagacttcc cagaaaccgc cgacggcaaa tacggtcgcg
    13681 taaatcggca ggaagtaaat cgcgccgaac agcattttgc ccaacacgct cgcttcagac
    13741 gacatattga tgcccaaagc gtcggcaagg gtgtaatgcc agtcgttggc gatgttttgt
    13801 tgcagcaaat caggcgttaa cgcaccgaat gcctgtacgc cgacgttgta cataccgtag
    13861 aacatggcag ggaacaaagc cagccacacc aaaatcatca tgcgtttgga gtcgagcgca
    13921 tcgcggacgt gtgccgcttt gcgcgttacc gcaccggatg tatagaaaat cgtcgccgca
    13981 gcttcgtaga gggcatacca tttttcatgt ttgccgcctg gcaggaagtg cggttcgatt
    14041 ttttccagaa aatgtttcaa gcccataatc agccttcctt ctcaatggtt tccagcacct
    14101 tacgcaacag cgggccgtat tcgtatttgc ccgggcagac gaagctgcac aaagcgaggt
    14161 cttcttcgtc caattccaag caacccaatg cctgcgcgct gtcggtatcg ccgacgatta
    14221 aatcgcgcaa aagcagggta ggcaggatgt ctagcggcat tacgcgctcg taagtaccaa
    14281 tcggcaccat ggcgcggtcg ccaccgttga cggctgtcgt gaacttgaag agtttgtttt
    14341 tcaggaaatg accgagggtc gtacgcgtga tggagtattt gtccggctgc ggcgcaaccc
    14401 agccgaacag ctctttgctg cggccttctt cgataacgga aatctgattg tggtagcgtc
    14461 ccaaataatc gtgcgcgcct tgtgtaatcg cgccgttcaa taccgaaccg gaaatcacgc
    14521 ggttgtctgc gtcaaccaat tcgcccgcag taatttgcga tactttcgca cccaaaacgg
    14581 tacgcaagag gcgtggtttg ttgacttgag aaccacccaa agcaatcacg cgctcggtgt
    14641 tcagacggcc tgttgcaaac aaacgtccga tggcaattac atcttgataa ttgatggtcc
    14701 aaacggtttt gtttgcaccg accggctcaa tgaaatgaat gtgcgtgcca ctcaaaccgg
    14761 ccggatgcgg gccgccgaat tcatgtgttt cgatgttggc agcattttca gacggcacgt
    14821 ctgcgccagc tgccttacac acatggattt tacgctcggt caaacggctc aataccagca
    14881 aacctcgtct gaaatcctcg gcggcttctt tgatcacaac cacagggtct gccgccagcg
    14941 gattggtgtc catcgcattg acgaagatgg cgaacggctc ggcatcgacg gcagggattt
    15001 tgctgaacgg acgggtacgc agcgcagtcc acaaaccgga ttggatcaga ttgcggcgca
    15061 cttcttcgcc gcttaagttt gccaacgctt cgggcgcgta gcgttcgaac tcgatttcgt
    15121 cgttgccttc aacggcaatc acgaccgact gaagtacgcg cttttcgccg cgatggatgg
    15181 cggcgatttt gcctgaaacc ggcgcggtaa acaccacgcc cggattcttt ttgtcttcaa
    15241 acagcacttg gccttttttg acggcatcgc cttccttgac tttcatcgag gggcgcatac
    15301 cggcatattc ttcgccaagc aacgcgactt cggtaatggc cgggccgtca taaatgactt
    15361 gctccggtct gcccgcgatg ggcaggttta gacctttttt gattttaatc atatatttgc
    15421 attacttgtg atggttaagg taaaaacggc gtgttttgat accgtgtcgc gtggcatcaa
    15481 aagcattgaa taaattaatg tagcaaagtg ttagattcta tcaggaattg tacctgtttg
    15541 tcagatttgc tgcttttttc cttgcggaag ccgtttttat agtggattaa atttaaacca
    15601 gtacggcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa
    15661 gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
    15721 ttaatccact ataattgtcg gaagggggga tattgatttg attatgccgg aatttaaaat
    15781 gccgtctgaa tgttcagacg gcatagcgtt tacagcagtt tgaaaacgaa aaagataagg
    15841 gtatgtacga tgaagacggg tgtcaggaag gcgaccgacc acatcatata gccgaagaaa
    15901 gtcggcatcg gtacgccgcg ctgttcggca atggccttga ccatgaagtt cggtgcgttg
    15961 ccgatgtagg tcagtgcgcc catgaatacc gaacccatag aaaccgccag cagcgaatga
    16021 aacagggtac ccgtcatcaa ggcttgggca tcgccgcctg ccatattgaa aaaaacgaga
    16081 taagtgggcg cgttatccaa gaatgccgac aatatgccgc tcatccaaaa atacatcaca
    16141 ttaatcggat gacctgccgt atcgtgaacc agcgatacca ccccgcccag cgcgcctgcc
    16201 tcgcctgctt tcagaatgct caggacggga aagatggtga tgaagatgcc gaggaagagt
    16261 ttgcccactt cggcgatggg ttcaaagttg aattcgttgc ctgcgcggac ttgtttgggc
    16321 gtgattgcca tagatacggc ggtcaatgca atcaggatga catcgcggac gaggttttgc
    16381 agggcgtaac ggctgccgag gatttcaaat cccgggtgtt cgggtttcca aaggccggac
    16441 attagaaccg cgccgaccac gcccgaaagc aggaggaagt tccatttgcc gaagatggcg
    16501 attttttcgg gtttttcctg ttgtgccggc gtatcttgtg caatgctttc ctgtttgaag
    16561 aaacggttgt cgatgaaata gaaggcggtc aacaggacag cggtgctgat caggacgggg
    16621 gcgaacatat gtttgaccgt ccacatgaaa tctacgcctt tgaggaagcc gaggaagagt
    16681 ggggggtcgc ccaaaggggt cagaccgccg ccgatgtttg caaccaagaa aatgaagaag
    16741 atgacgatgt gcacgcggcg ggtacggttt tggttggctt tcagcagcgg acgaatcatc
    16801 agcattgctg cgccggtcgt tcccatgata gaggcaagtg cagtaccgac ggcaagcagg
    16861 gcggtgttga gcttgggtgt gccgttcaag tcgccccaaa ccaaaatgcc gcctgaaatg
    16921 gtgtacaggg caagcagcag caggatgaag gggatgtatt cttcaacgag tgcgtgtgcg
    16981 acggtatgga taccggcgga cgcgccaaaa accaaactga acgggatgag gaagagcaat
    17041 gtccaaaagg cggtaatttt gccgtaatgg tgatgccagg tatgcgaaaa aaacaaggga
    17101 cccaatgcga tagacagcaa aatcagggca aagggcaggc cccacagcag gtttaggttt
    17161 gcgccgtcca aatctgcggc gtaaaccgat gctgggaaaa gcattagtga aaacaggggt
    17221 aggtggcgca tcgtgtttcc tcgattcaag cactgccttg cgcggcgcgt gggagtgata
    17281 caggcaccgt gccgcccgga cataggcgga ctgtgctgcc tgtttgtttt tgaaaaaagt
    17341 tttccgattg attgtaaagt aaataatcag gataaaccag tttcccaaac cggaaggcgg
    17401 cgggaaggcg gattgccgtg cttgggaata tatgttcttt ttgataaata attttattta
    17461 aaacaaataa atatatgtct ttaataaata atctatcgaa aacgaaaaat gaatttattt
    17521 taacatatat ttgcaatgaa acaggtttgc cccccgtttg tttgccctta tccctttcag
    17581 tacggcattc aagattcggg cctgcgccac atccatatgg cgacaaggga acaaaaaacc
    17641 gatgaaaccg ccccgaccca ccagcgttgg ggaaactgcc aaaacattat caggcaggat
    17701 gcggtcatca tactgatggc gaatattttg gctttgcgcg gcactgcgcc gttttgttcc
    17761 cagttatgaa ccatcggacc gaaatagcgg tgccggtgca gccagcggtg aaagcgcggg
    17821 gatgcctttg cccagcaggc ggcggagagc agtacgaacg gcgtggtcgg caacagcggc
    17881 aaaaaaatgc cgatgatacc caacagcagg gaaatgcagc cgcaggcaat taaaagataa
    17941 cgtatcattt tgaaatattt ttcttattgt gcggataagg gcaggatgtg ataccgagtt
    18001 ttgcccagcc ttcatgtccc attttttcca gcagggcgat attgcgttcg aatattgccg
    18061 aagcgtcggg aaaggcttgt gcggctttgg caatgctgtc ttcgcggatc agatgcagtg
    18121 tcggataggg agaacggttg gtgtagttgc cgatgtcgtc gggttcggta ttctgaaagc
    18181 ggaattgggg atggaacggt gcgatttgga ggatgccttc caagccgttt tcgacaacgg
    18241 cggcatcggc aatatcgagc atatcgttga atacgtcgaa atcggggaat agggtcgggt
    18301 gaaccagcag ggtggtttcc agttcggtgg cgggtgtatt gcccagtcgc tgcagttctt
    18361 cgtccaagtc ttccaaaaaa ccgtcaaggt gtttggcttc gctgatcgcg atgcggacaa
    18421 ggtttttaac gtggggggct ttggcaaagg gacacaggtt cagaccgatg acggcttttt
    18481 ccaaccattg tccggtgtgt tcggcaacag catctttatt ttcggaagta ttgatattca
    18541 ttattgtcat gtaaatgtgt ttgcagattg cacgtgcggg aaaatcggga agggcactat
    18601 tccttcagca ggtggttgag tggcagggag gtggtgtgtt tgatttcttt taaaacaaag
    18661 ctcgattggg catcctgtac gccgtggtgg gacaggagcg tatccaaaac aaaatgggaa
    18721 aacgcgttca tatcggtaaa aaacgcctga agcaggtagt cggtttcccc tgtcagggcg
    18781 aagcagctca agacttcagg ccattttcga accgatgcgg caaagtcttc ccgcgcgtct
    18841 tttgctttgc ggatggaaac gcggataaat gcctgaagcc ccaggttgac ggattccgga
    18901 gacagcagcg cggcatattg gcggacgatg ccggcatctt ccaactgctt cagacggcgc
    18961 aggcacggag aaggcgaaag tgcgacacgt tcggacagtt cgacattggt cagcctgccg
    19021 ttttcctgga gaacctgtaa gattttaata tcggttttgt ctaaagtgag ttggggcata
    19081 tttgcgttcc gttttaagga attcggattg tctgtccgta tgtttgcggc aatccgcaca
    19141 gatggagacc atattaacat ataaaaagtt ataccgtcat ccgggacaaa ttttgttttc
    19201 ggaaaatcat gtgaaaacag aggcggtcgg tttgcatctc ttttaagacg gcttgcccaa
    19261 accgccgatt caagacataa tcgggaaatg tgcaggagag tgttacaccc aactacaatg
    19321 taaccaccga aggcgcagac acccttaaat cgctcaggta tcagggactg cacattgaaa
    19381 caaacaatct ggagagcggc gttggaataa cgtccaccga aggggagaag gccgtctgaa
    19441 ccaccattca gacaaccgcg caaagcagtg agcagactgg tttgccatca tgcggatacg
    19501 gccgaaaatc tcaggttcaa ggacagatag ggtcatccgc gcacaggtgc gcgggtggca
    19561 tctgaacaaa aaatccggag aaacttgaga atgattactc tgaaaaccac cccatttcat
    19621 caagcccatc aagatgcagg cgcgaagccg gtagattttg ccggctggga gctgcccatc
    19681 cattatggtt cgcaaatcgc cgaacacgaa gccgtgcgca ccgacgccgg tatgtttgac
    19741 gtatcccata tgcttgttac cgacgtagca ggcgcaaatg ccaaagcctt tttccgcaaa
    19801 ttgattgcca acgatgtcgc caagctcgct tttgtcggca aagcccttta ttccgctttg
    19861 ctcaacgaca acggcggcgt gattgacgac ttaatcgttt accgcaccaa cgaagccgaa
    19921 acccaatacc gcatcgtgtc caacggcgcg actcgcgaaa aagatacggc acaattccac
    19981 aaagtcggac aagagttcgg tgtttccttc aatccgcgct acgacctcgg catgctcgcc
    20041 gtacaaggtc ctaaagccat tgaaaaactc ctgaccgtca aacccgaatg ggcagatgtc
    20101 gtccataacc tcaaaccgtt ccaaggcgcg gatttgggca acgactggtt tgtcgcccgc
    20161 actggctaca ccggcgaaga cggcgtcgaa gtcatcctgc ccggcaccga agccgtcgca
    20221 ttcttcaaag ccctgcaagc agcaggcgta caaccttgcg gtctcggcgc gcgcgacacc
    20281 ctgcgcatgg aagccggcat gaacctctac ggcaacgata tggacgacga taccagcccg
    20341 ctcgaagcag gcatgggttg gaccgttgat ttgaaagacg aaagccgcga ttttgtcggc
    20401 aaagctgcct tgttggcatt gaaagaaaaa ggcgttgccg tcaaacaggt cggtttgctg
    20461 ctcggcaaag gcggcatcct gcgcgcgcat atggaagtgt tgaccgacaa aggcaaaggc
    20521 gaaaccacca gcggcgtatt ctctccaagt ctgaaacaat ccatcgccat cgcccgcgta
    20581 ccgaaagatt ttgacggcga taccgccaaa gtgctgattc gcggcaagga agcggacgtg
    20641 cgcgtactga agctgccgtt cgtccgcaac ggtcaaaaac agtttgatta atatcggttc
    20701 agacggcatt ttcatttcat atgccgtctg aaagcaggtt ttaattgttg tccgatacgg
    20761 acgtttgtag aaagcattga acaaggcatc tgtggatatt gattcatgca gatgccgtct
    20821 gaaaataacc cctatcaatg gagtatcaaa ccatgagcaa caacatcccg gccgaactga
    20881 aatacgttgc cagccatgaa tggctgcgcc ttgaagaaga cggtacgatt accgtcggca
    20941 ttacccacca cgcgcaagag ctgttgggcg acatcgtgtt cgtcgagctg cccgaagtcg
    21001 gcgcaaacct tgccgctgaa gagcaagccg gtgtggttga gtctgtaaaa gccgcgtccg
    21061 acgtgtacgc accgattgca ggtgaagtcg ttgccgtcaa cgatgactta cccagtgcac
    21121 ctgaaactgc caacagcgac ccttacggtg caggctggtt cttcaaaatc aaacctgtca
    21181 atcctgccga ttacgacggt ctgctgactg ccgaacaata cgcgggcgaa gtagattaaa
    21241 ccgcccggct gcccgacggc aaccgccgga caaacggaaa ctgcaccttc agacggcatt
    21301 tttgcggtcg gaggtgcagt tttttgtccg tgttttaagg aagcagttag gctataataa
    21361 cggtctatat tcatctttac cgattttttc atgcaactta ccgctgtcgg actcaatcat
    21421 caaaccgcac ctttaagcat acgggaaaag ctggcgtttg ccgccgccgc cctgcctaaa
    21481 gccgtccgca atcttgcccg aagcaatgcg gcaacggagg cggtaatcct ttctacctgc
    21541 aaccgcaccg agctttactg cgtcggtgat tcggaagaaa tcatccgatg gcttgccgat
    21601 taccacagtt tgccgattga agaaatccgt ccgtatctgt acgcgctgga tatgcaggaa
    21661 accgtgcgcc atgccttccg cgttgcctgc ggcttggatt cgatggtgtt gggcgagccg
    21721 cagattttag gacagattaa ggatgcggtc agggttgctc aagagcagga aagtatgggt
    21781 aagaaactca atgccctgtt ccaaaaaacc ttttctgttg ctaaagaggt ccgtaccgat
    21841 actgccgtcg gcgaaaactc ggtttccatg gcttccgctt ccgtcaaatt ggcggaacag
    21901 atttttcccg acatcggcga tttgaatgtc ttgtttatcg gcgcaggcga aatgattgag
    21961 ctggttgcca cttattttgc cgccaaaagt ccccggctga tgacggttgc caaccggacg
    22021 ctggcgcgtg cacaggagtt gtgcgacaag ctcggtgtca acgccgaacc gtgcctgctg
    22081 tccgatctgc ctgctattct gcacgattac gacgtggtgg tttcttcaac ggcgagccag
    22141 cttccgatag tcggcaaagg catggtcgaa cgtgcattga aacagcgtca gagtatgccg
    22201 ttgtttatgc ttgatttggc cgtgccgcgc gatattgaag cggaagtcgg cgatttgaac
    22261 gatgcctatc tttatacggt ggacgatatg gtcaacatcg tccaaagcgg caaggaggca
    22321 aggcagaagg ccgccgccgc cgccgaaacg ctggtatccg agaaggttgc cgaatttgtc
    22381 aggcagcagc agggcaggca gagcgtcccc ttgattaaag ccttgcggga cgagggcgag
    22441 aaagcgcgca agcaggtgtt ggaaaatgcg atgaaacagc ttgccaaagg cgcaacggca
    22501 gaagaggttt tggaacggct gtccgtccaa ctgaccaaca agctgctgca ttcgccgacc
    22561 caaactttga ataaggcggg ggaagaagat aaagatttgg ttcatgccgt cgcgcagatt
    22621 tatcatttgg acaaataacg gtgcgccggg aaaaatgccg tctgaagagg tttcagacgg
    22681 catttttttg tgccgcctga caacatcgtg aaatcccaca ttatatcgat gtaatcacaa
    22741 agtatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt
    22801 acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactatatt atcccgtatg
    22861 cggattggtt ttaagatttg taaatttaat ttgcatcaaa aaatcgccga tagatgattc
    22921 atataatatc aatattaaag agtatcggta tatcggggat agtcatgtcc tgtttttcaa
    22981 tcaaacgtat gtccgcgttt cgggcgcgga taacggcgtt ttttgccgcc tttgtctttt
    23041 tgacggcggc actgcccgct tatgcggagc gtctgcctga ttttctggcg aaaatacagc
    23101 cttcggaaat ttttccgggt gcggaccgtt acggcaagcc ggaaggtaag cctatggttg
    23161 cccgcgttta caaaggcgat gagcagttgg gcttggtcta tatcacgacc gatgcggtca
    23221 atacgcgcgg ttattcgagc aaaccgattg atacgctgat ggcgttggca aacgacggaa
    23281 cgatagccgg agcgaaattg gttgatcacc atgagtcgat tatgctgatc ggtatcccgc
    23341 atttgcccgc gcccgggcgg gcgatacgct caaactggct tccggcgtat atcaaaccaa
    23401 actttatatc gacaaaccga ttacgattga agggcctgcc gaccgttctg caaccatcga
    23461 aggggacagg agcgggcgta ccatagccgt acacgcgccg gacgtaacgc tccgcaacct
    23521 gaccgttacc cgttccggca tgagcctgcc agcaatggat gccggtattt atctcgaaga
    23581 aactgccccg cgcgccctga ttgaacacaa caatattttg gataattcgg tcggcgtcta
    23641 tctgcacggt tctgccgatg cgatggtgcg cgagaataaa atcgtcggcg acgcgacttt
    23701 gcgcgtgaac gagcgcggca acggcgttac cgtttggaac gcacccggtg cgcaggtcgt
    23761 cggcaacgac atttccaaag ggcgggacgg cattttttcc aacaccagca cgcacaacac
    23821 ctataaaaac aaccgcttca gcgatttgcg tttcgccgtc cactatatgt acaccaacga
    23881 cagcgaagtc agcggcaata tttccgtggg caacaatatg ggctgagacc tttgcaataa
    23941 cataggttac taaaatttta tgctcaatct cattttcaaa atgcaaaact tttctgattt
    24001 ttcctacttt ttgctcaata ttaggaaggt tttaggcaat tgaaaatttt ttggcgcatt
    24061 tttatgcgtc aaatttcgtt aacagactat ttttgcaaag gtctcgggct atgtgctgat
    24121 gttttccgaa cggctcaaag tattcgacaa tattgccgtc ggtagtcgcg actagggcat
    24181 catgctcaac tatgtcaact attccgatat tcacgacaat attatcaaca aagcgggcaa
    24241 gtgcgttttt gcctacaatg ccaactacga taaactgtcc gccaatcatt ttgaaaactg
    24301 ccaaatcggc atacacttta ccgccgccat cgaaggcacg tctctgcacg acaattcctt
    24361 tatcaacaac gaaagccagg tcaaatacgt cagcacgcgc tttctcgact ggagcgaggg
    24421 cggacacggc aactattgga gcgacaacag tgcgttcgat ttgaacggcg acggcttcgg
    24481 cgacagcgcg taccggccca acggcatcat cgaccaaatc atctggcgcg cgcccgtatt
    24541 gcgcctcttg atgaacagtc tcgcaatcag catcgtcaaa tgggcgcagg cgcagtttcc
    24601 cgccgttctg cctagcggcg tggtggacag caaaccgctg atgaagcctt atgcccccaa
    24661 aattcaaacc cgttatcagg cgatgaagga cgggctgctc aaagaagtcg aaacgcggca
    24721 gttggaatgg ggcagggcgg aaaacggttc tttgaactag tctgcttcag acggcatccg
    24781 gattcaaatg ccgtctgaaa acacaaaagg aacaaccatg accacacatc atgtcgaatt
    24841 gaggaaggta accaaacgct tcggcgcgca aaaagccgtc aaccaagtcg atttggtttt
    24901 gaaggcagga gaaagcgtcg ggcttgccgg acacaacggt gcgggcaagt ccaccatcat
    24961 gaagctgata ctcgggctga ttaccccgac cgaaggcgaa gtgatgcttt tgggcgaacg
    25021 taccggcagc aaagcggggg cgcggcttcg cagccaaatc ggctacctgc ccgaaactgt
    25081 tgcgctgcac ccttcgctga ccggcatcga aacgctggat ttttacgcca aacttaaaaa
    25141 acagccgctc acgcagaacc gggggctgct cgagcgcgtc ggtatttcac aggcggcaca
    25201 ccgccgcgtc ggcacttatt ctaaagggat gcgccaacgc cttgccttgg cacaagccct
    25261 gctgggcgag cccaaagtcc tgctgtttga cgaaccgaca accggtcttg accctgcatc
    25321 acgacaaatg ttttacgaag tcgtgcgcga actcaacggg cgcggcgcga ccgtattgct
    25381 cagcacccac gcccttgccg agttggacgg gcacgccgac cgcattatcg tggattaaat
    25441 ttaatccact atatgcgggt atggcgggtt tgagcggaca aatcagcctg accgtccccg
    25501 ttttgctgac cgctcaggtt ttatgggtta tcattccgct tgttttggca gccggaattt
    25561 ttagaaagcg acaaatatga agaaaaccct gttggcaatt attgccgttt tcgccttaag
    25621 tgcctgccgg caggcggaag aggggccgcc gcctttaccc cagcagatta gcgaccgttc
    25681 ggtcggacac tattgcagta tgaatctgac cgaacacaac ggccctaaag cccagatttt
    25741 tttgaacggc aaacccgatc agcccgtttg gttctccacc atcaagcaga tgttcggcta
    25801 taccaagctg cccgaagagc ctaaaggcat ccgcgtgatt tacgttaccg atatgggcaa
    25861 tgttaccgat tggacgaatc ccaatgccga cacggagtgg atggatgcga aaaaagcctt
    25921 ttacgtcatc gacagcggct ttatcggcgg tatgggtgcg gaagacgcgc taccgttcgg
    25981 caacaaggag caggctgaaa aatttgcaaa ggataaaggc ggtaaggttg tcagtttcga
    26041 cgatatgcct gatacctata ttttcaaata aatccaaaca aaaataccgt ctgaacgttg
    26101 aagccaaggt tcagacggca ttttttatag tggattaaat ttaaaccagt acagcgttgc
    26161 ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttaagtt
    26221 taatccacta tattatgggt tcaaacgggg caggtttgaa caactgcccc attccttatt
    26281 cctgaccggg cgtaacggcg gaataaggtt tacatcccgc cgtaattcgg cccgctcgcg
    26341 ccttcggggc aaatccaagt gatgttttgc gtcgggtctt tgatgtcgca ggttttgcag
    26401 tgcacgcagt ttgccgcgtt gatttgcagg cgcggattgc cgttttcttc aataatttcg
    26461 tacacacccg ccggacaata gcgcgtttcg ggcgaggcgt attctttgta gttcacgttt
    26521 atcatcgttt gcggattgtt cagcaccaaa tggtcgggct ggttttcttc gtgcgcgaga
    26581 ttggcaagga agacgctgct caagcggtcg aaggtcaaca cgccatcggg tttcggataa
    26641 gcaatcggct tgcacgcggc ggcttttttg agctgctcgt tgtctttacc gtgatgtttc
    26701 aaggtccacg gggctttgcc tctgaaaatc atctggtcga tgccggtata aagcgagccg
    26761 aggtaaacgc cccatttgaa tgacggacgg acgttgcgcg cggcgtaaag ctcttgatac
    26821 agccagcttt gttcaaaacg ttgctgataa tccgccgcct ctttgccgct gtcgaaaccc
    26881 tccacttctt caaggttttc caacaagggg aacacggctt cggcggcgag catggcggat
    26941 ttcatcgcgg tatgaatgcc tttgatgcgc ggcatattga ggaaacccgc cgcatcgccg
    27001 accaaaatgc cccctttgaa cgagagtttc ggcaggcttt gcaaaccgcc ttcaatcagc
    27061 gaacgcgcgc cgtaagcaat gcggcggccg ccttcaaagg ttttgcggat ttcgggatgg
    27121 gttttgaaac gttggaactc ttcaaacggc gacagataag gattttgata gtccaaaccg
    27181 accacgaagc cgacggcgac tttgttgtca tcgaaatggt aaacaaacgc gccgccgtag
    27241 gttttgctgt ccagcggcca gcctgcgctg tgcaccacca aaccgggctg atgctgttcg
    27301 gacggcactt cccaaacttc tttaatgccc aagccgtaag tttgcggctg gctgttttgg
    27361 tcgagtttga agcgttcgat gatttgtttg gaaagcgaac cgcgacaacc ttcggcaaac
    27421 agggtttgct gcgcccaaag ctccatgccg ggttggaatg aatcggtcgg ctcgccgtct
    27481 ttgccaatgc ccatattgcc ggttgcaatg cctttgaccg aaccgtcttc gtgatacagc
    27541 acttcggcgg cggcaaagcc cggatagatt tccacgccca tattttccgc ctgctccgcc
    27601 aaccagcgca cgacttcgcc caagctgacg atgtagttgc cgtgattgtc gaaatttggg
    27661 gtaataggca gattgaacgc ttttttctca gtcaggaaca acactttgtc ctgcgttacc
    27721 gtacgcgtca gcggcgcgcc tttttctttc cagtcgggaa tcaactcatt cagcgcaatc
    27781 ggatcgataa ccgcgcccgc cagcgaatgt gcacccgcct ccgaaccttt ctccaccacg
    27841 caaacgctga tttcgcgccc gttctgttcg gcaagctgct tgagtttgat ggcggcggac
    27901 aaacccgatg ggcctgcgcc gacaatcacg acatcgtatt gcatactgtc gcgggtgatg
    27961 gattctgtca tggcggttcc tgtgtattta ttattgaatt gcaaatccgt aattatacaa
    28021 cgggaacata tagttaccaa atacaacaaa ggtcgtctga aaaccatatt ttcggttttc
    28081 agacgacctt tgtcgaaatt tcaataagca cgccaccatt ttacctgtcc gaccgcaaac
    28141 tccgtctgac gtttcggact gcgtgtgaaa aacgccttat ccccgccggc atccctccct
    28201 ttcggcac
//
LOCUS       DAWFJW010000015        27284 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFJW010000015 DAWFJW010000000
VERSION     DAWFJW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.015"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(213..587)
                     /locus_tag="WGR19_000539"
                     /pseudo
     CDS             complement(213..587)
                     /locus_tag="WGR19_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(893..2152)
                     /gene="rho"
                     /locus_tag="WGR19_000540"
     CDS             complement(893..2152)
                     /gene="rho"
                     /locus_tag="WGR19_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0798120.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILSDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(2372..4756)
                     /gene="ppsA"
                     /locus_tag="WGR19_000541"
     CDS             complement(2372..4756)
                     /gene="ppsA"
                     /locus_tag="WGR19_000541"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0798121.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISVALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITHRIAGYASPIDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSIYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5167..5988
                     /locus_tag="WGR19_000542"
     CDS             5167..5988
                     /locus_tag="WGR19_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0798122.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(6228..6890)
                     /locus_tag="WGR19_000543"
     CDS             complement(6228..6890)
                     /locus_tag="WGR19_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0798123.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6903..7730)
                     /locus_tag="WGR19_000544"
     CDS             complement(6903..7730)
                     /locus_tag="WGR19_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0798124.1"
                     /translation="MKPDIYALLERALLSGNPDEKGRLADEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRTFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSATCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
     gene            8008..8631
                     /gene="lolA"
                     /locus_tag="WGR19_000545"
     CDS             8008..8631
                     /gene="lolA"
                     /locus_tag="WGR19_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0798125.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            8904..10043
                     /locus_tag="WGR19_000546"
     CDS             8904..10043
                     /locus_tag="WGR19_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0798126.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEATNGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            10176..11246
                     /locus_tag="WGR19_000547"
     CDS             10176..11246
                     /locus_tag="WGR19_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0798127.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKTTNNKQQTTNNKQQTTNNKQQTTNNKQQTTNNTRYRTPNIKYIYQDN
                     QGTAEARNTGIKNSNGKYIVFIDSDDFINCQVLLDFLSKDDIDMPDVVFLNAVKYDKG
                     SVSHFGEDYQPEKILNQSKVEVLKGLCRFRKFPGSACNKIIKRELIIKEKLFFEKEVY
                     AEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDSKTGTVSEKSIKSLLYILEKNAEME
                     FNRDISSYLYSFLSYEYLVLLFIMTSKNIACDADIKRRAYHLRFMLLKSNKLIYKLIF
                     PIITLFGVDITGRILKAIRGNI"
     gene            11287..11817
                     /locus_tag="WGR19_000548"
     CDS             11287..11817
                     /locus_tag="WGR19_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0798128.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(11828..11966)
                     /locus_tag="WGR19_000549"
                     /pseudo
     CDS             complement(11828..11966)
                     /locus_tag="WGR19_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(11985..13580)
                     /locus_tag="WGR19_000550"
     CDS             complement(11985..13580)
                     /locus_tag="WGR19_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0798129.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LKIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLSIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(13687..14082)
                     /gene="hisI"
                     /locus_tag="WGR19_000551"
     CDS             complement(13687..14082)
                     /gene="hisI"
                     /locus_tag="WGR19_000551"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0798130.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(14113..14880)
                     /gene="hisF"
                     /locus_tag="WGR19_000552"
     CDS             complement(14113..14880)
                     /gene="hisF"
                     /locus_tag="WGR19_000552"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0798131.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAANPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(14893..15630)
                     /gene="hisA"
                     /locus_tag="WGR19_000553"
     CDS             complement(14893..15630)
                     /gene="hisA"
                     /locus_tag="WGR19_000553"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0798132.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(15663..16301)
                     /gene="hisH"
                     /locus_tag="WGR19_000554"
     CDS             complement(15663..16301)
                     /gene="hisH"
                     /locus_tag="WGR19_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0798133.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(16432..17934)
                     /gene="pta"
                     /locus_tag="WGR19_000555"
     CDS             complement(16432..17934)
                     /gene="pta"
                     /locus_tag="WGR19_000555"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0798134.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(18119..19177)
                     /gene="fbpC"
                     /locus_tag="WGR19_000556"
     CDS             complement(18119..19177)
                     /gene="fbpC"
                     /locus_tag="WGR19_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0798135.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(19198..20715)
                     /gene="fbpB"
                     /locus_tag="WGR19_000557"
     CDS             complement(19198..20715)
                     /gene="fbpB"
                     /locus_tag="WGR19_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0798136.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(20783..21778)
                     /gene="fbpA"
                     /locus_tag="WGR19_000558"
     CDS             complement(20783..21778)
                     /gene="fbpA"
                     /locus_tag="WGR19_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0798137.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(22029..22499)
                     /locus_tag="WGR19_000559"
     CDS             complement(22029..22499)
                     /locus_tag="WGR19_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ0798138.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWSFPAAEKTCSVLKKIGAVHSAMLIHRAADALGKEIRHLQS
                     EGKNLKEMWDITQHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(22557..23933)
                     /gene="argH"
                     /locus_tag="WGR19_000560"
     CDS             complement(22557..23933)
                     /gene="argH"
                     /locus_tag="WGR19_000560"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0798139.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(23952..24821)
                     /gene="galU"
                     /locus_tag="WGR19_000561"
     CDS             complement(23952..24821)
                     /gene="galU"
                     /locus_tag="WGR19_000561"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0798140.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(24849..25448)
                     /gene="rdgB"
                     /locus_tag="WGR19_000562"
     CDS             complement(24849..25448)
                     /gene="rdgB"
                     /locus_tag="WGR19_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0798141.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDSA
                     NNLKLVGELACKADKSCCYVCVLVFIRHENDPRPIIAEGVWHGQWHDTPQGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(25441..25674)
                     /locus_tag="WGR19_000563"
     CDS             complement(25441..25674)
                     /locus_tag="WGR19_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798142.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAKENRYV"
     gene            complement(25782..26315)
                     /locus_tag="WGR19_000564"
     CDS             complement(25782..26315)
                     /locus_tag="WGR19_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0798143.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(26417..26875)
                     /gene="nudB"
                     /locus_tag="WGR19_000565"
     CDS             complement(26417..26875)
                     /gene="nudB"
                     /locus_tag="WGR19_000565"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0798144.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            27085..27161
                     /locus_tag="WGR19_000566"
     tRNA            27085..27161
                     /locus_tag="WGR19_000566"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27119..27121,aa:Pro,seq:ggg)
BASE COUNT         5906 a         6666 c         8004 g         6708 t
ORIGIN      
        1 ggtatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
       61 acggaaccca ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
      121 gaggcaccgc cgtactggtt tttgttaatc cactataaaa atttatgggg ctgtcctaga
      181 taactaggat aaactcgatt ttactaattg ttttaaaatg gaacaagaac ttttatctca
      241 ctgttgttaa aacgtcgttc gtaccccttt aaatacagct caaaatgcgc tttgggaatg
      301 ccgtcaaact tgcgtaaatg acgttttgcc cggtttcaaa agttcccaat tccattgata
      361 tggttttgtc gttcagcaaa ataactttca tctgcttcta ctttgccgtc aaacatttcc
      421 aaatgcggac tgttttgata aataagtaat cgtaaacgat gaaaataata ggctgcggta
      481 tttttattaa cgcctactaa ctctgctgct gttcttgcag ttacacctgc gacaaacagt
      541 tcaatgagtt tattttgttt atactggctt agacgacttt ttctcatagg ggcaactcta
      601 acttaatttg aatttcccta gttatctagg acagccccaa atttatacaa aaatgagcgc
      661 ggttcggcgc aaccttgaat caagttcccg catcggtttt cattgccggt acggatgcgt
      721 tcaagccggt tttgcaaagg ccgcgctttc ggcaagcgga cacggacact gccgacggtt
      781 gcgccgttaa cggggggagg gaggagctgc gccgaccgtg tgaatgaaag tgccgtctga
      841 aacccgattt tcaggcttca gacggcattt cgcattaatg cgggcggcgc gtttatttgc
      901 cgcgcatcag ttcaaagaaa tcgtcgttgt ttttagaggc tttgattttc ccgtttaaga
      961 actcggtagc ttcgatttca tccatcgggt gcaggaactt gcgtaagagc cacatacgtt
     1021 gcaactggtc gttcggcaca agcagctctt cgcggcgcgt gccggatttg ttgatgttga
     1081 tggcggggaa gaggcgtttt tccgccatac ggcgatcaag gtgcaattcc atattgccgg
     1141 tgcctttgaa ttcttcgtaa atcacatcgt ccatacggct gccggtttca accaatgcgg
     1201 tggcgataat ggtcagcgaa ccgccttctt ccacgttgcg cgccgcaccg aagaaacgtt
     1261 tggggcggtg cagcgcgttg gcatcgacac cgccggtcag gattttgccc gaggtgggga
     1321 cgacggtatt gtaggcgcgg gcaaggcggg taatcgaatc cagcaggatg accacgtctt
     1381 ttttgtgttc caccatacgc ttggcttttt caagcaccat ttcggcaact tggacatggc
     1441 gttgagccgg ctcgtcaaag gtggaggaaa ccacttcgcc acggacggag cggctcattt
     1501 cggttacttc ttcgggacgt tcgtcaatca agaggacgat gagttcgact tcgggatagt
     1561 ttgcggtaac ggcgtgggca atgttttgca gcatcacggt tttaccgctt ttgggcgggg
     1621 caaccaagag ggcgcgctga cctttgccga tgggggaaat caggtcgatg gcgcgtccgg
     1681 tcaggttttc ttcggacttt aagtcgcgtt ccagcttcaa ctgttcggtc ggaaacagcg
     1741 gggtcaggtt ttcaaacagg attttatggc ggcatacttc cgggtggtcg ccgttgatgg
     1801 tatcaagcct gaccagggca aaatagcgtt cgttgtcttt tgggacgcgg acgctgcctt
     1861 cgatggtgtc gcccgtatgc aggttgaagc ggcggatttg ggtgggcgag acatagatgt
     1921 cgtcggggcc ggcaagatag gacgtgtccg cgctgcggag gaagccgaag ccgtcggaca
     1981 ggatttcaag cgtgccggag caggtgaaac cctcgccttt tttcatcatt tggcggacga
     2041 tggcaaatac gaggtcttgt ttgcggaatc ggttggcgtt ttcgatgccg tgttcttccg
     2101 ccaattctaa gagtttggaa atgtgcaggg tttgtaattc ggagacgtgc ataataatga
     2161 tgtattttga agaggaaaaa gacaggcaga tgccgtctga aagaagaagc tgaccgttgc
     2221 cggttgctcg gggaaggggg aattgtaggc agtcggcgcg tgggtgtcaa atattatcgc
     2281 ggacggggca tcggcaggaa atgccgtctg agcggagctg cttggaaaaa aatacccccg
     2341 cgcttttcag gctcgggggt atgggcattg attatttgtt caattcattc gccaaatata
     2401 gccaagtttc gatgacggta tccgggttca gggaaacgct ttcaatgcct tcctcaacca
     2461 gccatttggc gaagtccgga tggtcggacg ggccttgacc gcagatgccg acatatttgt
     2521 tctgcttgcg gcaggcggag atggcaagat gcagcatcac tttgacggca gggttgcgtt
     2581 cgtcaaacga ttcggatacc aagccgctgt cgcggtcgag accgagggtc agttgggtca
     2641 tgtcgttcga gccgatggag aagccgtcga agtattgcag gaattgttcc gccaataccg
     2701 cgttgctcgg cagctcgcac atcataatca ggcgcaggcc gtttttgccg cgttccaagc
     2761 cgttttcttt cagggctttg acaacggctt cggcttcgcc caaagtgcgg acgaacggaa
     2821 tcatgatttc aacgttggtc agacccattt cgtcacgaac gcgtttcaag gctttgcatt
     2881 cgagggcgaa gcagtctttg aaactttcgg cgacataacg cgccgcaccc cggaagccca
     2941 acatcgggtt ttcttcgtgc ggttcgtaga tgctgccacc caccaagtta gcgtactcgt
     3001 tggatttgaa gtcggacata cggacgatgg ttttacgcgg ataaaccgat gcggctaaag
     3061 ttgccacgcc ttctgcgatt ttatcgacat agaagtcaat aggagaggcg tagccggcga
     3121 tgcggtgggt aatttcagct tttaactcgt cgtcttgttt gtcaaattcc aacaaggctt
     3181 tggggtggat gccgatttgg cggttgatga tgaattccat acgcgccaag ccgatgcctt
     3241 cgctcggcag gttggcgaag ctgaatgcga gttcgggatt gccgacgttc atcatcactt
     3301 tgacaggtgc tttgggcata ttgtccaagg cgacatcggt aatctgtacg tccaacaggc
     3361 cggcatagat gaagccggta tcgccttcgg cgcaggatac ggtaacttcc tgaccgtttt
     3421 tcagcaattc ggttgcattg ccgcagccga cgacggcagg aatgcccaat tcacgcgcga
     3481 taatcgctgc gtggcaggta cgtccgccgc gattggtaac gatggcggaa gcgcgtttca
     3541 tcaccggttc ccaatccgga tcggtcatat cggtaaccag tacgtcgccg gcttcgacgg
     3601 aatccatctc ggaagcatct ttaatcaggc gtactttgcc ctgtccgact ttctgaccga
     3661 tggcgcggcc ttcgcacaag acggtttttt cgccgttgat agaaaagcgg cgcaggttgc
     3721 ggctgccttc ttcttgggat ttcacggttt cgggacgggc ttgcaggata tagagtttgc
     3781 cgtccaagcc gtcgcgaccc cattcgatat ccatcgggcg gccgtagtgt ttttcgatgg
     3841 tcagcgcgta atgcgccaac tcggtgattt cttcgtcggt aatggagaag cggttgcggt
     3901 cttcttcggg gacatcgacg ttggttaccg atttgccggc ttcggctttg tcggtaaaaa
     3961 tcattttgat gtgtttcgaa cccatggttt tacgcaggat ggcgggcttg cccgctttga
     4021 gcgtaggttt gaacacataa aattcgtccg ggttgaccgc gccttgtacg acgttttcgc
     4081 ccagaccgta agaggaggta acaaagacga cttgatcgta gccggattcg gtgtcgaggg
     4141 tgaacatcac acctgatgcg ccactgtcgg aacgcaccat gcgttgaacg ccggcggaaa
     4201 gggcgacgat gtcgtgttcg aagcctttgt ggacgcggta ggaaatggcg cggtcgttgt
     4261 acagggaggc gaatacatgg cgcatcgctt ctttaacgtt ttccaagccg ttgatgttca
     4321 agaaggtttc ctgttgtccg gcaaatgatg cgtccggcag gtcttcggcg gttgcggaag
     4381 agcgtacggc aacggaaatg tccgcaccgc cggcatcggc aaccattttg ttccatgccg
     4441 cttcgatttc ggcatcgagc tgttcgggga agggcgtgtc caaaatccat tggcggattt
     4501 ctttgccgac gcgtgccagt tcggcaacgt cttcgacatc caattttgcc agtgcgacgg
     4561 aaatgcgttc gctcagaccg ttgtgtgcga ggaatgcgcg gtaggcatcg gccgtggtgg
     4621 caaagccgcc ggggacgcga acgccttttt cggtcagctg actaatcatt tcacccagcg
     4681 aggcgttttt accgcccacg cgttcaacat ctgtcatacg caggttttca aaccagatta
     4741 cgtagttgtc ggccatttgt gtgtccaatc caaaatatgt taaaaaagaa acaaatccgc
     4801 gtgcttattt taagcgattc gttcctctgc tgtcatgtgt tttatccgtt ttaaaatcat
     4861 gatgccgtct gaaaaattgc ggtttcggcg tgtgtagcgg tttgaaactt gcagccggta
     4921 tacttctttt tttgggtatt ttctttgtaa aacaggtggt ttgaataggt taatgttttt
     4981 tctgtttgat ttttttgttt attttttaaa attttctgcc aaaaaatact ttatataaat
     5041 aattttattt caaaattata ttgtgtctgt ttgggtgtaa tccgaggtag gcgtgctgcg
     5101 ggtgctttcc ttgtgtctgc cgctgctgtt atgatgatat ttttattctg tatttaagga
     5161 tggaagatga gcagtccgcg ccatgtgttt tacatttccg accgtaccgg tctgactgcc
     5221 gagaatatcg gcgaggcttt gctgaaccag tttggcaatc tgtcgttcaa acgccatacg
     5281 catccgtttg tcgatacgcc ggaaaaggcg cgcgcggtgg tggagaaggt caatcggagc
     5341 cggcaggaaa acggtcagcg tccgattgcg tttgtcagtg tggttgatga cgaaatccgt
     5401 cggattatca aaggggcgga tgcttttcag attaatttct ttgagacttt tttgggactg
     5461 ttggagaaag aactcaatac cgaagccacg gcatccgagc aggggcatca cagtatcggt
     5521 aatacgaagc gttacgatgc gcgtatggag gcggtaaatt tctcattgaa ccacgatgac
     5581 ggggtcagcg ataagaacct tcaggaagcg gatgtaatct tgatgggtgt atcgcgttcg
     5641 ggcaaaacgc cgacctgcct ttacctcgcc ctgcaatacg gcatccgtgc ggcaaactat
     5701 ccgctgattc ctgacgattt ggaatcggcc gatctgccgc gtatggtcaa gccttatagg
     5761 gataagctgt tcgggttgac catccagccg gaacgtttgc aggccatccg ccaagagcgc
     5821 cgcccgaatt caacttatgc caaaatcgat acatgccgca gcgaggtggc agacgcgcag
     5881 agtatgttca gacggcatgg gattccgttt gcgaatacga cggataaatc agtggaagag
     5941 cttgccgtac acatccttca ggcgtgcaag ctcaaacgca ggttttgacg ggttttgatt
     6001 cggtttgaag gtggaaatgc cgtctgaaat caggtttcag acggcagttt tatagtggat
     6061 taacaaaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggagccgatt
     6121 cacttggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgctg
     6181 tgctggttta gatttaatcc actatatgtt tgtggggcgg atatttttca gggctgtatt
     6241 ttgtccagac attcgagcag atcgagcggc gttcggatgt ggaaatccgc ctgccatgag
     6301 tcggtatcgt cttcatctga gatgtagccc cattcggcga ggacggtttt catgccggcg
     6361 ttgcggcctg cttggatgtc gcgttcggca tcaccgacgt agagtgtgtg ttgcgggtcg
     6421 gcgtggattt gtccgcacgc atacagcatg ggtttgatgc tgggcttggg ttcgccgcag
     6481 gtgtcgccgc tgacgacgac ggcgggtggg atgatgaagc cgagtttggg gacgagtttg
     6541 tcggtgaagc gcatgggttt gttggtgatg atgccccatt tgatgccgcg ttggttgagt
     6601 tcggcaatga gttcgttcac gccgtcaaag agctcggtgt cttgggcgta gcggcggtcg
     6661 tattcctcca gatattcggt gcgccatcgg gcatagtcgg ggtggtcggg ggtgatgcct
     6721 gcgccgagtt tgatcagtcc tgccgcgccg tggctggctt gggtgcggat ctcgtccatg
     6781 ctttttgcag gtagtccgtg gcgagcgagc agggtgttga gtgcgcctcc gaggtcgagg
     6841 gcggtgtcgg caagtgtgcc gtcgaggtcg aagagtacgg cgtgtatcat gtgtgttcct
     6901 tttcagacgg tatttttgtt ctgtttcaaa ccctgctcga aatcttccag catatccaat
     6961 tcaaagcggc tgaagcccgc tttttcgcgc gcttcgatgt tcacatagcc gcggaagata
     7021 aacatatcgt aacgggcaat cagactgcgg aacagggtaa tcggttccaa accgcgttcg
     7081 cggcagaggt gttgatacca atggttgccg atggcgacat gacccacttc gtcgcggtaa
     7141 atgatgtcca acacgccgca ggttgccgaa tcgccgcgct gcgccacctt cgcgcgtatc
     7201 cccggcgtaa cgtccagccc gcgcgcttcc aacacgcgcg gcaccaaagc catacgcaac
     7261 agtgggtcgt aggcggtttt gtacgccata tcccacaaat ggttgtgcgc ctcgaaactg
     7321 ccgtaatcga agccgaaagt gcgcaatctg tcgcgcatca ggcggaaatg gtacacctct
     7381 tccttcgcca ctttcaccca gtcgcggaca aactgaaacg gcagcgtgcg gaaacggtat
     7441 gccgcgtcca aagccagatt gacggcgttg aattcgatat gcgcaatcgc gtgcagcatc
     7501 gccgcatagc cttctgccgt gttcattttg cgcggggtca gttgcgacgg cgcgaccaaa
     7561 acaggcttgt ccggccgtcc cgctcggggg aagtccgccg gcggcgcgtt tgtttccgcc
     7621 ccgtccgcat tttgaacggc ggcaaacgcc tcatccgcca gccgtccttt ttcatcgggg
     7681 ttgcccgaaa gcagggcgcg ttccagcaaa gcataaatat cgggtttcat ttcaagtccg
     7741 ccgtgttcgg aaaacaaata ttatagcgtt taaaaaaaac aagatgaggc atataatctc
     7801 cgcgattcgg cattccgcgc ccaaaccgtc aaatcacgcg gcacacaatt aaagggcagc
     7861 gtggcgcgcc taccttttcc gagcggtcaa aaaaatcagc cctcggaaaa cgcggtttgc
     7921 aaaatgcaaa ccgcccgtaa cgccgcccgt atgattgttt tgctgcgccg atactttacg
     7981 ccacacccat cccaacaagg aaaaataatg atgaaaccgc acaacctgtt ccaatttctc
     8041 gccgtttgct ccctgaccgt cgccgtcgct tccgcacagg cgggcgcggt agacgcgctt
     8101 aagcaattca acaacgatgc cgacggtatc agcggcagct tcacccaaac cgtccaaagc
     8161 aaaaagaaaa cccaaaccgc gcacggcacg ttcaaaatcc tgcgcccggg cctcttcaaa
     8221 tgggaataca cttcgcctta caaacagact attgtcggcg acggtcaaac cgtttggctc
     8281 tacgatgtcg atttggcaca agtgaccaag tcgtcccaag accaggccat aggcggcagc
     8341 cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca gctacacgct gaaagaggac
     8401 ggttcgtcca acggcatcga ttatgtgctg gcaacgccca aacgcaacaa cgccggctac
     8461 caatacatcc gcatcggctt caaaggcggc aacctcgccg ccatgcagct taaagacagc
     8521 ttcggcaacc aaacctccat cagtttcggc ggtttgaata ccaatcccca actctcgcgc
     8581 ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt tgagcaactg atgccgtccg
     8641 ccccgatgcc gtctgaaagc cgccgaggct tcagacggca ttttcacgca ggcggaacaa
     8701 tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa cccgtgaaaa ttaacggttg
     8761 cgccccggct gtttttgccg tttaatgcaa accttgctgc accaagggcc aagaaagccg
     8821 accggccgcc cccacagctt ccgatgcagg cggcccggcc gtccctgcaa tgttttttat
     8881 ttttgaacga aaggtcgaaa accatgaaaa aaacactggt ggcggcggca atcctgagcc
     8941 tcgccttgac cgcgtgcggc ggcggaagcg ataccgccgc ccaagccccc tccgccaagc
     9001 ccgaagccga acaatcgggc aaactcaata tctacaactg gtcggattat gtcgatcccg
     9061 aaaccgtcgc cgcctttgaa aaagaaaccg gcattaagac gcgttccgat tattacgaca
     9121 gcaacgaaac actggaggca aaagtcctga ccggcaaatc cggctatgac ctgactgcgc
     9181 cgtccatcgc caacgtcggc cggcaaatca aagcgggcgc gtatcagaaa atcgacaagg
     9241 cgcaaatccc ccattacggc aacatcgata aagatttgct gaaaatgatg gaagccgtcg
     9301 atccgggcaa cgaatacgcc gtcccctatt tctggggcat caacaccttg gcaatcaaca
     9361 cgcggcaggt gcaaaaggca ttgggtacgg acaagctgcc cgaaaacgaa tgggatttgg
     9421 tgttcaaacc cgaatacacc gccaaactca aatcctgcgg catcagctat ttcgacagtg
     9481 caatcgaaca gattcctttg gcgttgcact atttgggcaa agaccccaac agtgagaatc
     9541 ccgaagacat caaagccgcc gtcgatatga tgaaagccgt ccggggcgac gtgaaacgct
     9601 tcagttcttc cggctatatc gatgatatgg cggcaggcaa cctgtgtgcc gccatcggtt
     9661 acggcggcga tttgaacatt gccaaaaccc gtgccgaaga agccacaaac ggcgtggaaa
     9721 tcaaagtatt gaccccgaaa accggcgtgg gcgtgtgggt ggattccttt atgattccgc
     9781 gcgacgcgca aaacgttgcc aacgcccacc gctatatcga ctacacgctc cggcccgagg
     9841 tggcggcgaa aaacggcagc ttcgtcacct acgcgcccgc cagccgtccc gcgcgcgagc
     9901 tgatggatga aaaatacacc tccgacgcat cgattttccc gaacaaagaa ctgatggaaa
     9961 aaagtttcat cgtatcgccc aaatccgcag aatccgccaa actgggcgtg aagctgtggc
    10021 aagggctcaa agcgggcaaa taaccggaat ccctgccgtc tgaaaccttt cgggcggcag
    10081 gaaacggcgc gtccgttatc aaacaggggg gcgtttcccc tcctgccggt tatgattggg
    10141 ttaagattaa aatgatttag taaaatgaga aagatatgga tttaagtatc gtagttccca
    10201 tttataatgt cgaaagttat ttggaagcgt gtctaaattc catagaacct atattaagta
    10261 atgaaaatgt cgaacttatc cttgtgaatg acgggtcaaa agacggaagt gaagatatat
    10321 gttataaata tatagataaa acaacaaaca acaaacaaca aacaacaaac aacaaacaac
    10381 aaacaacaaa caacaaacaa caaacaacaa acaacaaaca acaaacaaca aacaacacca
    10441 gataccgaac accaaacatc aaatatatat atcaggataa ccaagggacg gcagaagcta
    10501 ggaataccgg aataaaaaat tcaaacggaa aatatatagt ctttattgat tcagatgatt
    10561 ttattaattg tcaggtcttg ctggattttc ttagtaaaga tgatattgat atgccggatg
    10621 tggttttttt aaatgcggtt aaatatgata agggaagtgt ttcacatttt ggcgaagatt
    10681 atcagcctga aaaaatactc aatcaatcca aagtcgaagt tttgaaagga ttatgccgat
    10741 ttagaaaatt tccgggttcg gcgtgtaata agattataaa aagggaattg attatcaaag
    10801 aaaaactgtt ttttgaaaaa gaggtttatg ccgaagatat cgaatggtca atgagattat
    10861 ttaatgcggc aacaactttt tcttatttgg acggttgtta ttactattat cggcagggaa
    10921 gaaaagattc taaaacagga actgtttcgg aaaaaagtat aaagtcatta ttatatattt
    10981 tggagaaaaa tgcggaaatg gaatttaata gggatatatc aagttatctt tattcttttc
    11041 tttcctacga atatctcgtt ttgcttttta tcatgacgag taaaaatata gcgtgtgatg
    11101 ctgatataaa aagaagggcg tatcatttaa ggtttatgct gttaaagtct aataagttga
    11161 tatataagct gatattcccg ataatcacat tattcggggt tgatattaca ggcaggattt
    11221 taaaagcaat cagggggaat atttaataaa tcctttaaca ctatacatct taccgaagga
    11281 ggaaaaatga acgcaatccg aactttccaa aaccgcacgc ccgaaatcca cgaaagctgt
    11341 atgatagacg aagcgtgcgt cgtcatcggc gaagtgtcgc ttgccgaaga cgtttctgtg
    11401 tggccgtgcg ccgtgttgcg cggcgatgtg aacagcatca ccgtcggcgc gcgcagcaat
    11461 atacaggacg gcagcgtcct gcacgtttcc cacaaaaccg ccgccaaacc cgaagggtcg
    11521 ccgctggtta tcggcgaaga cgttaccgtg gggcacaaag tgatgctgca cggctgccgt
    11581 atcggcaacc gcgtcctggt cggcatgggg acgacggttc tggacgatgc cgtgattgag
    11641 gacgaagtga tgatcggcgc gggcagcctc gtgcctccgc gcaaacgttt ggcgggcggc
    11701 tatctttatg tcggttcgcc ggtcagacaa gtgcgcgtgt tgaccgatga ggaaaaagcc
    11761 tttttgaagt attccgccgc gcattatgtg aagctgtcga aacagtacgg gatgtgaaat
    11821 cacatcggcg ttcttgcgtc agccccaaat tcatgcggat gggacgcatc cgataacggt
    11881 atccgatgcg ccttgatttt gatcggtgca tttgagttgc aggcaaaaat gccgtctgaa
    11941 gccctttttt cgggttcaga cggcatttta ttgccgattg ctttttaaag tttgaccgaa
    12001 tgctcgcgcg tttcgtggaa cacgatgtcc ggccaacgtt cttgcgtgag tcccaaattc
    12061 acgcggttgg gggcgaggta ggcgaggttg ccgcctgcgt ctatcgacag attgcctgca
    12121 ttggcttttt caaattccgc cagttttttc ttgtcgtcgc acgataccca gcgcgccgac
    12181 cagatggatg cgctgtcgaa cacggcttct acaccgtatt cgttggcgag ccgcgaggtt
    12241 acgacttcaa actgcaacac gccgaccgcg cccaaaatca aatccgcgcc gctcatcggt
    12301 ttgaacacct gtaccgcgcc ttcttcgcca agctgttgca aacctttttg cagttgcttg
    12361 attttcagtg ggtttttgat gcggacgctg cggaacagtt cgggcgcgaa gaagggaatg
    12421 cctgtaaatg ccagttgttc gccttcggag aagctgtcgc cgatttggat gttgccgtgg
    12481 ttcgggatgc cgatgatgtc gccggcgtag gcttcttcca ccagctcgcg gtcgtgcgac
    12541 atgaaggtta ccacgctgga ggcggcgatt tcgcggttga tgcgaaggtg tttcatcttc
    12601 ataccgcgct cgaatctgcc ggagcagacg cgcaggaagg cgatgcggtc gcggtgtttc
    12661 ggatccatat tggcttggat tttaaagata aatccggaga atttcggctc gtccggctcg
    12721 accatacgta cggtcgcgtc gcgcggtttc ggcgcgggcg cccagtcaat caatgaattg
    12781 aggatttcct gaataccgaa gttgttaatc gcagagccga agaatacggg cgtgagttcg
    12841 ccggcgagga attcgtcgag attgaactcg ttggaagccg cctgcaccaa ttcgatttcg
    12901 tcgcgcaact gttggatttc caatggaaag cgttgttcca attcaggatt atcgatacct
    12961 ttgatgatgt cgaattcgtg cggcaggcgt tcgccgcccg cttcaaagag atagatttcg
    13021 tcgttcagga tgtggtacac gcctttgaag tttttgccca taccgattgg ccaggtaacg
    13081 ggcgcgcagc ggatttttaa aatgttttcc acttcgtcta aaagttccag ggaatcgcgc
    13141 acttcgcggt cgtatttgtt catgaacgta acaatcggcg tatcgcgcag gcggcagacg
    13201 ttcaagagtt tgatggtttg cgcttccacg ccttttgccg cgtcgatgac catcaacgcg
    13261 ctgtccacgg cggtcaaaac gcggtaggtg tcttctgaga aatcttggtg tcccggcgtg
    13321 tccaagaggt tgacggtgtg gtctttatag tcgaactgca tcacgcttga tgccacggaa
    13381 atgccgcgct gcttctcgat gtccatccag tcggaagtgg cgaatttgcc ggtttttttg
    13441 ccttttaccg tacccgcgct ttgaatcgca cctgaaaaca gcaagagttt ttcagtcaac
    13501 gtggttttac ctgcgtcagg gtgggagatg atggcaaacg tgcggcggcg gcgcacttgg
    13561 tcgaggattt cttgggacat ggttttcttt gcaaaaaggt tcaggccgct tttcagacgg
    13621 cccggacagt gtttgagacg gcgaaattgt acaaaaaaat gcctgataat tcaatgttgg
    13681 aggcggtcag tgcgtgctgc cgtaaatctc tttttcgtct ttcaggacgg catcggcggt
    13741 ttcccacgcg ccgccgcgcc agactttgta aaagcagctt tcgcgcccgg tgtggcaggc
    13801 gatgccgccg ttttgggcga tgagcatcac aatggcgtcg ccgtcgcagt cgaggcgcag
    13861 ttcgcggact ttttgcgtgt gtcccgactc ttcgcccttc atccattgtt tttggcgcga
    13921 acggctgtaa tagtgggcaa agccggtttc gacggttttt tgcagggctt cggcgttcat
    13981 ccacgccacc attaaaacac gtttggtttc ggcatcttgg gcgatggcgc aaaccaaacc
    14041 tttttcgtca aatttgacgg cttcaagcag gtttttatcc atatttcctt tcagacggca
    14101 tagtcggggc ggtcagaggc gcacttcgat gccggcttcg cgcatagcgc gtttggcttc
    14161 gcggatggcg atttccccga aatggaaaat gccggcggca agtacggcat cggctttgcc
    14221 ttcggttatg ccttcaatca ggtgccggac attgccgacc ccgccggagg cgatgacggg
    14281 gatgtcgacg gcttcggcaa cggcgcgggt cagcggcagg ttgaaaccct gtttcgtacc
    14341 gtccctgtcc ataccggtaa gcaggatttc gcccgcgccg cgtttttgca tttcgaccgc
    14401 ccattccacc gcatccaaac cggtcggatt tcgcccgccg tgggtaaaga tttcccagcg
    14461 tgtgttttcg gggttggcgg ctttggcatc gacggcggcg acgatggctt gcgaaccgaa
    14521 aaatccggcg gcttcgtcaa ttaaatcggg acgggtaacg gcggcggtgt tgatgctgac
    14581 tttgtccgcg ccggcattga gcaggcggcg gatgtcggca acggtgcgta cgccgccgcc
    14641 gacggtcagg gggatgaaga cttgtccggc aacttcttcg atgatgtgca ggatggtgtc
    14701 gcggttgtcg gatgaggcgg tgatgtcgag gaaggtcaat tcgtccgcgc cttcgccgtt
    14761 gtagcgtttg gcggcttcga cggggtcgcc cgcgtcgcgc aaaccgatga agttcacgcc
    14821 tttgacgacg cgcccgtctt ttacgtcgag acaggggatg atgcgttttg ccagtgccat
    14881 aatcggatgc ctttagtcga gggaatctgc cagttgctgc gcttgggcaa aatcgatgct
    14941 gccctcgtaa atcgcgcggc cggtaatcgc gcctgctacg ccatgttttt cggcggcaca
    15001 cagggcgcgg atgtcgtcca agccggtcag tccgccggag gcgatgacgg gaatgcggac
    15061 ggcttgggcg agtttgaccg tcgcgtcgat gttcacgccg ctcatcatac cgtcgcgccc
    15121 gatgtcggtg tagatgatgc tgttgacgcc gtcgtcttca aagcgttttg ccaaatcaat
    15181 tacatgatgc ccggttacgg ttgcccagcc gtcgatggcg gccataccgt ctttggcatc
    15241 cagcccgaca ataatcctgc cggggaaggc tttgcacgcc tcgcgcacga agtcggggtt
    15301 tttgaccgcc gccgtgccga taatcacgtc gttcagaccc aaatccaaat attgtccgat
    15361 ggttttcaaa tcgcgtatgc cgccgccgag ctgtacgggg atgtctttgg cgacggcggc
    15421 aaggatgtct ttgatggcgg gcaggttttg cggaacgccg gcaaacgcgc cgttcaaatc
    15481 taccagatgc aggcggcgcg cgccttgttt gaaccagtgc agtgcggttt cggcgggcga
    15541 atcggaaaag acggtcgcct cttccatcag cccttgtctc aggcggacgc agcgtccttc
    15601 tttcaaatcg atggcgggta tcagcagcat aatttttctc cttgtgcggg gccgtgtccg
    15661 gcttaccagt ttaaaaagtt tttcaacatc gtcagcccgg catcgtggct tttttcggtg
    15721 tgaaattgcg tggcgaatac gttgtctttg ccgacgatgc aggcaaacgg ggacgggtag
    15781 tcgctttcgc ccaatatggt ttcgggattt tcgggggcga aatagtagct gtggacgaag
    15841 taaaaacgcg tgtcttgggg aatatcttta aacagcgggt ggttttgggt ttggcgcacg
    15901 gtgttccagc ccatatgcgg gactttcaga cggcatccct gcgggtcgcg gaggtcgcgc
    15961 tcaaagcgtc tgactttgcc gccgaaccag cccaagccgt cggtgtttcc ttcttcactg
    16021 tggtcgaata aaagttgcgc gccgacgcag attccgaaaa agggtttgtt ttttaaggca
    16081 tctttgactg cctcgtccag gccgccgcgc gtcagtgccg ccatacagtc gggcatcgcg
    16141 ccctgcccgg gaaaaatgac tttgtcggcg cgggacacgc gttcggggtc gccgcttaaa
    16201 aagatttcgg tattttttcc ggcaagctgc cccgccgtcc ggacggattt caatacggaa
    16261 tgcaggttgc ccataccgta atcgataatg gcgatttgca tggcttcctc ctcttttttt
    16321 gcaatatggc tgcgatttta acaaacaaat gtgccgtgct gataaaaatg ccgtctgaaa
    16381 acgggagtct gtcttcagac ggcatagggt ttaaacccgg aaagccgttt gtcagccttc
    16441 catttgtttt gcctgaacgg cagtcagggc gatggtgaac acgatgtctt ccaccagcgc
    16501 gccgcgggag aggtcgttga ccggtttgcg caggccttgc agcagcgggc cgacgcttaa
    16561 gacgttggca ctgcgttgga cggctttata ggtgcagttg ccggtgttca ggtcggggaa
    16621 gaccaaaacg gttgcctgtc ctgccaccgg gctgcccgga gctttggatt tgcccacacc
    16681 cggcacggtt gccgcatcat attgcagcgg gccgtcgatg gcgaggtcgg ggcgtttttc
    16741 ccgggcaagt ttggttgctt cgatgacggt atcgacatcg gggccgctgc cggagttgac
    16801 ggtggagtag gaaatcatcg ccactttcgg gtcgatgccg aaggcttttg cggaatcggc
    16861 agactggatg gcgatgtcgg caagctgttg cgcggtcggg ttcggattaa ccgcgcagtc
    16921 gccgaagacg aggacttggt tgggcagcag cataaagaat acgctggaca cgaggcttgc
    16981 gcccggtgcg gttttaatca gttgcaaagc ggggcggatg gtgttggcgg tggtgtgaac
    17041 cgcaccggat accaaaccgt ccacatcatt ttgcgccatc atcatcgtac cgagtaccac
    17101 ggtgtcttgc agttgcttgc gcgcgtcttc gggtgtcagg cctttggatt tgcgcagttc
    17161 gcacatcggc tcgacgtatt gttcgaccaa tgaggcggga tcgatgattt ccaaagagtc
    17221 gggcaggctg atgccgcgtt ctttggcaac ggcttcgact tcttcgcgtt tggcaagcag
    17281 gacgcagcgg gcaatgcctt tttcgtggca gatggcggcg gcttggacgg tgcggggttc
    17341 tgcgccctca ggcaggacga tgcgtttgtc ggcttggcgg gcgaagtcga tcaggttgta
    17401 gcggaattgc gccggcgaca ggcgttttgc ttcgcggcct gccaatacgg atacgtcttt
    17461 cagcgcgtcg ctcgaaccga agaaggtcag gccggttttt tcggctgccg cttcggcaac
    17521 ggaggctgcc gcgccgtcca cgacaaaacc ttccaatacg cccggcgcgg cggcgaagaa
    17581 ctgtttggca aggttcagcc gatttgccag ttcgtcggca tcggcgttgt cggaacggac
    17641 ggcgaagacg gctgccgcgt caagggacaa tgccagttcg acgtttttgc ctgcgaggta
    17701 gattttgtcg gcatcgggcg cgatgccttc gatgacgagg ttggcggcat cgagtgcggc
    17761 aactttgccg accagtgcgt cgaaccagtc gtcgcttttg ccttgcgcga gcagggtttc
    17821 ggcggttgcg tcaacggctt ggaaaatttg tgcgtccagt gcttttgcaa aggcttgtgc
    17881 ggcggcggag gcgtccagtc cggcagatac gggtacgatg agtacttttg ccatgatata
    17941 tcctttcgta tgctgcggtg tgcggcatat gtggttggaa ggggcggcat ataggcagaa
    18001 acggctgcct gcgtgccgtg cgtgccgtgt ttggcttgag gcgcgcaggt tgaatatagc
    18061 aaacaaattc tgtttccaac aagataaata tccgcaggct tgtggatgct gccgcctttc
    18121 agagggtatt tccggggaag aacagggcgg gaccgtccaa atggaggacg gcggaaatgc
    18181 cgtctgacag ggtgggggcg gaagggaggt tgagcgtgag gacggtttgt ccgacccgga
    18241 gactgatttc ggtatgccgc gctttgggcg tggttttgag aaccacggcg tgaatggagg
    18301 cggtgggtgc ggaatggggg tgaaggctga actgttccgg acggatgagc agtgtgccgc
    18361 gtgtgcctgc gggtgcgccg ctttgaacgg gcaggcggcc caatccgcaa tcggcggtgc
    18421 cgtcggcgtt gagcgcggcg gggaacacga tgccttcgcc gataaacagg gcggcatcaa
    18481 ggtcggcagg ttgtcggtac aattcgtgag ggcttgcagt ttggaggatg cgcccctgtt
    18541 tcatcacggc aatccggtcg gcgtattgca gggcttcttc gcggtcgtgg ctgacgaaaa
    18601 cggcagattt gccgttggcg cgcagggcgg caatcatgtc ttcgcgaatc tggcggcgca
    18661 actgttcgtc cagcgcgctg aagggttcgt ccaacaaaat cagttcggga tcgggtgcga
    18721 gggcgcgggc gagggcgacg cgctgttgct gtccgcccga aagttcgtgc ggatagcgtc
    18781 cggcaagttc ggaaatgccg gtcaattcca acatagcttc gatgcgctgc cgctcttgcg
    18841 ccgtcttgcc tttgccgttg cccagcccgt aggcggtgtt gcggtaaacg gtcaggtggg
    18901 ggaacagtac gccttcctgt acgacataac ccaaacggcg ttcgcggacg gggaggttgg
    18961 tatttttcga gaagatggtt ctgccggaaa gcgaaatttc gccaaaatcg ggttgttcaa
    19021 aaccggcaag gcagcgtaaa agggtggttt tgccgcagcc ggacgcgccg acgataaaga
    19081 ggatttcgcc cgggtcgagg ctgagcgaaa tgtcgtttaa aaccggggtg ttttgaaaac
    19141 ttttggacag gtgtccgatg tgcagggcgg cggtcatggc ggtacttcct caagctgtta
    19201 tttgaaggcg tatttcttca gcaggaatac ggggatgccg gaaaataata ccagcatcag
    19261 cgcgtaaggg gtggcggcgg cgtattgtgc gtccgatgtg tattcccaaa cggcggtgga
    19321 gagtgtgtgg acatcgtcgg tggtcagcag cagggtggcg gtcagctctt tcatcagttt
    19381 gaggaagacg agtgcgaatg cggcggtaat gccgggcagg atggacggca gtaccaacgt
    19441 cctgaaaata aagaagtgtc cgcgccccaa tgttgcgccg acctgttcca tccctttggg
    19501 gagttgttcc aaggaagtcc tcagggtggt ttgcgccatc ggcaggtaaa gcatgaaata
    19561 ggcaaggatg acgacgataa aggtttggta aacggcaggg gtgtagttga tgctgaaata
    19621 aaccaaggat agggcgataa ccaaaccggg gacggcgtgc agtaaaaacg gcagcctgtc
    19681 tatccaaacg gttaaaaaat tgcgatagcg aaccgatgcc caaacaaggg gcaaggcaca
    19741 taatatagtc aaaatcgcac ctaaagccga tacgcttaag gaacggataa aggcatcaaa
    19801 tacggatacg agcgcgaatg tgccggaagt gccgaccatc atccaatgta tcaatacgcc
    19861 aaaggggata ataatgccca aagtcaacaa gctgcttaaa aaaacaatcg cgccaatctg
    19921 accgggcagt ttgagggttt tgacgggata aggacgggca acgcctttgc cgctgtggta
    19981 aatcttggct ttgccgcgaa atatgctttc tccaaatacg acgatgccgc acaccgccat
    20041 taaaacagcg gaaagcaggg cggcggtatt gttgttgtag gacatttcgt attcttggaa
    20101 aatggcggtg gtaaaagtgg ggtagttcaa aatagatacc gcgccaaatt cgaccagcat
    20161 atgcagggca atcagtaaca cgctgctgcc gatggcgggt ttgagctggg ggaggatggc
    20221 ggaaaaaaag gtttgcaggc ggcttttgcc caaggacagg ctgacttctt cgtaagacag
    20281 gctgatgcgt ttgagtgccg cctcgacggg caggtaggcg agcgggaacg aggacaggct
    20341 cataatcatc actgtccccc aaaagccttc gacacggaag gtcaggctga tccaggtgaa
    20401 acagctgaca aatgcgggga tgcacaaagg cagggtgatt gccgtctgaa aaaaggtttt
    20461 gccgaagaag cggtaacgtt ggaacaaaag ggcgcaggca atgcccaaaa caatggaaat
    20521 cagggtaacg cccgccatca tcgtcaaggt gttggagagc aaatcccaca tacgcgggcg
    20581 gaacaacagt tcgacggcgc ggttgatgcc gacctgccac gaacgcatag cgacatataa
    20641 aaaaggcagg gtaagcggta gggcaatcag taggatgagg ccggtaagcc aaatgggtat
    20701 ttttttagga gacatagtgt tttttatcgg caaaacgggc ggacagtata aatgtccacc
    20761 cgtttgacaa tccgaaaacg gcttatttca taccggcttg ctcaagcagc cgggtggcgt
    20821 gttctttttc ggaaacagtg gtggcggaca cttggggtgc ttccaacttg gcgatgggtt
    20881 ccaaattgaa agtggatacc acgtgcggat tcaaaggata ttcggcacgg acggcggtca
    20941 aggcgcgctg tccttccttg ctggcgagga aggcgacgaa ttttttcgcc tcatccttgt
    21001 tttgggagga ttttaacacg gctgcgccgg aataggtaac gagtgcgccg ggatctctgt
    21061 ggcggacgaa attcaggcgg gtgtggacat tttgtacgcc tttttcacgc gcaaaagcgt
    21121 gccagtagta gttgttgatg agggcggcat cgatttcgcc gttttcaacc gcttgaaggg
    21181 cgacggagtt tttagcgtaa ggcttgccgt attctttcag acctttgagc catttcaatg
    21241 cggccgcttc gcctttcagt ttgacgatgg cgacaacctg ttccaagaac gcgccggaag
    21301 tgggggcgta accgatgcgg tttttccatt tcggcgtggc gtaattcagg acggattttt
    21361 ccaaatcttt ttcagacagt ttgcgggtgt cgtaaacgac gacgcgcgaa cgtccgctca
    21421 gtgccaccca gtcttttttg gcggcaaccg gcacgccctt gccgcgtgtt tcgttgatgg
    21481 tggaggcggg caggggctct aggaggttgg ctgcggaaag ggtggcgagt gccgggattt
    21541 gttcggaata gaatacgtcg gcggggcttc ggctgccttc ttctttgatt tgaccggcaa
    21601 gctggtcgcc tttggcgctg ttgagtttga ctttgatgcc ggtagcccgg gtaaaggcat
    21661 ctgcaacggc ttgtgccgct tctttgtgtt ggccgttgta cacggtaatg tctgccagcg
    21721 cgggggttgc ggcggtcagg gctgcggcaa gcagtgcgta tcggatagat gttttcatat
    21781 cgattttctc ctaatgaatg agagtgtaca ccttgttaag acataacggc gtgtagtgta
    21841 ttccttcttc tttataaatg caaataatta ttttttaaat ttgttattat ccgatccggt
    21901 tatggtttgt tctgacttgt attttttccg tgcatcgcgc ccgtaaggcg gaagcggcgg
    21961 gcaatgcgtg gcggaatgtg ggtaaaggcg gcattttgat ttgtcggaat gcttgagaac
    22021 ccctctcttt aaaacagcgt ctgaacaggg ttgccggaat agtattgcca tcccggcaga
    22081 tacagtttgt cggggtcttg ccaatattgt tcatccagac tgttcggcag tgaggcggtt
    22141 ttgttgtcga gatgttgcgt tatatcccac atttctttca ggtttttacc ttccgattgg
    22201 aggtggcgga tttcttttcc caatgcgtct gccgcccgat ggatcagcat tgcgctgtgt
    22261 accgcgccga tttttttcag aacggaacaa gtcttttcgg cggcggggaa actccagttg
    22321 cagacaaatt gcagtatgct gccgttaccg atatcggctt ctgtccgcca aatcaaaacc
    22381 agctcctgtt cccgcccgtc catgctttcg agtttgccgt catgctgttc aaaaagtttg
    22441 tccaccgcct gccacatcat ctgttcgaag cggtcggctt gggtatccgt atcggtcatc
    22501 gtgttcttgc cttttaaaaa tgccgtctga acatttcttc agacggcatt tgggggttaa
    22561 gccaacattt cccgccagcg tttgacttgg aaacggactt gttcaggcgc ggtgccgccc
    22621 aagtggttgc gcgcgtttaa gctgccttcg ggtgtcagca cgccgtaaac gtcgtcggca
    22681 atcaaatcgc tgaaaccttg taagacttcg agcggcagtt cgctcaaatc gacgccagct
    22741 tcatcggcgt ggcgcacggc ttgggcgacg acttcgtggg catcgcggaa gggcatacct
    22801 tttttaacca gataatccgc caagtcggtg gcggtggcaa aaccctgcat cacggcggcg
    22861 cgcatattgt cgggtttgac ggttacgccg cgcatcatat cggcgtaaat ccgcagcgtg
    22921 tcgatgagcg tgtcggcggt gtcaaacaag ggttctttgt cttcctgatt gtctttgttg
    22981 tacgccaagg gctgcgattt catcagggta atcaagccga taaggtgtcc gatgactcgg
    23041 ccggatttgc cgcgcacgag ttcgggcacg tcggggtttt tcttctgcgg catgatggac
    23101 gaacctgtgc agaaacggtc ggcgatgtcg ataaagccga aacgcggact catccacaaa
    23161 atcaattctt cagacaggcg gctcaggtga accataacca gcgaggcggc ggcggtgaac
    23221 tcgacggcaa aatcgcggtc ggatacggca tcgagcgagt tttggcagat ttgttcaaag
    23281 cccaatagct cggcggtaat ttcgcgctga atcgggtagg tcgtcccggc aagggcggct
    23341 gcgccgagcg gcatacggtt gacgcggcgg cggcagtccg ccatccgttc gttatcgcgg
    23401 ccgagcattt cgacgtaggc gagcatatgg tgtccgaagc tgacgggctg ggcgacttgc
    23461 aggtgggtaa agccgggcat aacggtttcg gcgttttgtt ccgccaaatc cagcaatgcc
    23521 gtctgaaggt tttgaatcag gcttcggata acggtaattt gatcgcgcag ccacaggcgg
    23581 atgtcggtgg cgacttggtc gttgcggctg cggccggtgt gcaggcgttt gcccgcgtcg
    23641 ccgattttgt cggtcaggcg gcgttcgatg ttcatatgga catcttccaa atcgagcgac
    23701 cattcgattt tgccgctgcg gatttcttcg aggatttccg ccataccccg acggatgtct
    23761 gccaaatcgc cttcgtccaa cacgccggtt tctttcaaca tttgcgcgtg ggcgagcgag
    23821 ccttgaatgt cccattcggc aagccgtcgg tcgaaaccga tggaggcggt gtattgtttg
    23881 acgagttcgg aaacgggttc gttgaaacgt ccggaccagg ttttgtcgtg cataaggatt
    23941 ccttgatggg gttattcggt gcggtatttt tccaaaaggc ggcggaaggg ttcgccggtt
    24001 tcggggtgtt tcagaccgta ggcgacggtg gcttcgaggt agcccagttt gctgccgcag
    24061 tcgtagcgcg tgccttcaaa ggggtgcgcc aggacaaatt cgtgatcgag cagcttggcg
    24121 atgccgtctg taagctggat ttcgttgccc gcgccgcgcg gcagtccggt cagtaagtcg
    24181 aaaatgcgcg gggtaaggat gtagcgtccg acgacggcaa ggttggaggg cgcgtcttcg
    24241 ggcttgggtt tttcgacaat gccgataatg cgttggaact gtttgagctg ttcggtttcg
    24301 acgatgccgt atgagccggt ttgcgatgct tcaacggttt ctacgcccaa aatgctgttg
    24361 ccgctgcgcc cgtacacttc gaccatttgt ttgagcgcgc ctttgggggc atcaatcagg
    24421 tcgtcggcaa ggataacggc aaagggttcg tctccgatgg cggcgcgggc gcacaagacg
    24481 gcgtgtccca agcccagtgc ttccgcctga cggatgtaga ggcaggtaat gttcggcggc
    24541 aggatgttgc ggacgtgttc caacaatttg tctttatggc gcatttccaa ctcggtttcg
    24601 agttcgtatg ccttgtcgaa atggtcttcg atgctgcgtt tgttgcgtcc ggtaacaaac
    24661 accatttccg tgcagccggc ttccacggct tcttctacgg cgtattggat cagcggcttg
    24721 tcgacgatgg gcagcatttc tttcgggctt gccttggtgg cgggcaggaa gcgtgttccc
    24781 atccctgcga cggggaagac ggctttccgt atgggtttca ttctttttcc tttgtattgt
    24841 tttgatgttt aaagggcgag tttgcggatt aaatcggcga gtgcctgcgc gcggtggctt
    24901 tcgcggtttt tgacctccga atccaattcg gcggcggttt tgccgtgttc gggcagataa
    24961 aaatacgggt cataaccgaa accgttttgc ccttgcgggg tatcgtgcca ctgtccgtgc
    25021 catacgcctt cggcaataat cggacgcggg tcgttttcat gacggataaa aaccaatacg
    25081 cagacgtagc agcagctttt gtcggctttg caggcaagtt cgccgacaag tttcaggttg
    25141 ttggcggaat cggatttggg gttgtcgccg gcgtagcgtg cggaatggat gcccggcgcg
    25201 ccgtttaggg cggcggcaca gatgccgctg tcgtcggcga gtgcgggcag cccgctgtat
    25261 tcggcggcat ggcgtgcttt tgccagcgcg ttttcgacaa aggtgggata gggttcgggg
    25321 cattcgggta tgccgaatgc ggattgcggc aatacggtga tgctgtaagg tttgaataag
    25381 ttgccgaatt cttcgagctt gccggcattg ccgcttgcca aaacgatttt ttccggtttt
    25441 tcagacatag cggttttcct ttgcggcgga ttgggcggcg cgtagggatt tgtgccgcag
    25501 gtagagggcg agtgcgccga tttgcccgaa cagtgcgccg aatgcgaaaa ggaaggaggc
    25561 gacggcgaag gctttgctgc cgacggtcag cagataagca cccaagagga cgagcagcat
    25621 aaatgccagt gtgaagaggg caacagacaa cagatagatt tttcggcggt tcatggcgtt
    25681 cggtcggaaa cggtatgttc ggattatatc cgattgggac ggtattccct agagcttgga
    25741 aaaatgccgt ctgaagcggc gttcagacgg catggcgggc gttatgcctg tttgttccag
    25801 cgttcgatgg attctttgat gacttttttc gcttcttccg catcgcccca agattcgact
    25861 ttggtcgtac ctgctttttt caggtctttg taatggttga agtggaactc gatttgtttg
    25921 atgagctgtt gcggcaaatc ggacaaagtt ttgtaggcgt tgccgttgtt gcggtcgtcg
    25981 gcaggaacgc agacgatttt gtcgtctact tcgccgtcgt caacgaattt catcacgccg
    26041 ataacgcgcg cttccaagaa tacgccggtt gccagaggtt gttcggtaac gagcagcacg
    26101 tccaattcgt cgccgtcttc gtccaaagtt tggggaatga agccgtagtt ggtcggtttg
    26161 gcgaagatgg cgggttcgac gcggtcgagt tggaatgcgg cgagtttgcg gttccactcg
    26221 attttgtggt tgctgccggc ggggatttcg ttgacgacgt tgatgatgcc gccgtccaca
    26281 tcgccggggg tcaggatttg gttgaagtct gccatttggt ttcctttgtt tgggaaagtt
    26341 tgaagtttga aagtatagca caaacgtccg gctgaaaatg cgcccgatgc cgtctgaaag
    26401 ggtgtacggg cgcgtgttac cgtttgccca aaaacctgcc cagttccaga atcgcgcgcc
    26461 tgttggacgg ggaaaacact ttttccgccg cttcttccaa gccgaaccag ccgtaggaga
    26521 cgtgttcttc gggttgcagg gcgatgggcg tatcacgcgg gatttcggca gagaagaggt
    26581 gttcgcggtt ttcaaacacg ccttttggat agcggtgccg ccagtggtgg tagatttcgt
    26641 aaaccgtgct gtcgtgccag tcttgaagct gcccgtccgc cagcaggatg ccggtttctt
    26701 cccaaacttc gcgccttgcc gtttgggcga cggtttcgcc cggttcgagg ctgccggtta
    26761 ccgactgcca aaatcctttc ggatgcgtgc gttcgatgag caggatgccg ccgtccccgc
    26821 tataaaggac gaccagtgcg gaaacggggt atttgagcgg ttttgccatc ggcatctttc
    26881 ggcgggctgc ggtaatgaag gggcggatta tagcaaacgc gcacgttatg gcgtttatcc
    26941 ttttccgtat ccttttttct gcacggatgg gacgcgccgg tgtttgccgg taaattttcc
    27001 gttgtgtcaa aaagataagg gcggttgtga ttttaatgct tgccaaagcg tcgggcggaa
    27061 actataatcc gaaacttatc gagtcggagt gtggcgcagt ctggtagcgc acttgcatgg
    27121 ggtgcaaggg gtcgaaggtt cgaatccttt cactccgacc aaaaattccg aaagccgctt
    27181 tcaaaagcgg cttttttgcc gtccgtatga ttatgatgta gagtacgcgg cgacagacat
    27241 tcaaataccg tccgaaaacc gttcagatgg catctcttta tctt
//
LOCUS       DAWFJW010000016        25604 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFJW010000016 DAWFJW010000000
VERSION     DAWFJW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25604
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.016"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            823..2271
                     /gene="gnd"
                     /locus_tag="WGR19_000567"
     CDS             823..2271
                     /gene="gnd"
                     /locus_tag="WGR19_000567"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0798145.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYSDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FTAWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGTDPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2334..3605
                     /gene="waaA"
                     /locus_tag="WGR19_000568"
     CDS             2334..3605
                     /gene="waaA"
                     /locus_tag="WGR19_000568"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0798146.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3646..4095
                     /locus_tag="WGR19_000569"
     CDS             3646..4095
                     /locus_tag="WGR19_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0798147.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            4128..5102
                     /locus_tag="WGR19_000570"
     CDS             4128..5102
                     /locus_tag="WGR19_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0798148.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSIVFGALGASILTSLIYTKKQSDK"
     gene            5171..5246
                     /locus_tag="WGR19_000571"
     tRNA            5171..5246
                     /locus_tag="WGR19_000571"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5204..5206,aa:Lys,seq:ttt)
     gene            5466..6719
                     /gene="murA"
                     /locus_tag="WGR19_000572"
     CDS             5466..6719
                     /gene="murA"
                     /locus_tag="WGR19_000572"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0798149.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGS
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYEHIEKKLGSVGAKIERVSG"
     gene            6800..7978
                     /locus_tag="WGR19_000573"
     CDS             6800..7978
                     /locus_tag="WGR19_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0798150.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFTADAEAV
                     VKDIADVAEDDMILDIGPKSAAELADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(8045..8293)
                     /locus_tag="WGR19_000574"
     CDS             complement(8045..8293)
                     /locus_tag="WGR19_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0798151.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8384..9301)
                     /gene="ftsX"
                     /locus_tag="WGR19_000575"
     CDS             complement(8384..9301)
                     /gene="ftsX"
                     /locus_tag="WGR19_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0798152.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9298..9948)
                     /gene="ftsE"
                     /locus_tag="WGR19_000576"
     CDS             complement(9298..9948)
                     /gene="ftsE"
                     /locus_tag="WGR19_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0798153.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9945..10136)
                     /locus_tag="WGR19_000577"
     CDS             complement(9945..10136)
                     /locus_tag="WGR19_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798154.1"
                     /translation="MPSEAASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(10144..10626)
                     /locus_tag="WGR19_000578"
     CDS             complement(10144..10626)
                     /locus_tag="WGR19_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0798155.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTEANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10750..11103
                     /gene="arsC"
                     /locus_tag="WGR19_000579"
     CDS             10750..11103
                     /gene="arsC"
                     /locus_tag="WGR19_000579"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0798156.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11100..11756
                     /locus_tag="WGR19_000580"
     CDS             11100..11756
                     /locus_tag="WGR19_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0798157.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPFLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKHPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11872..13266
                     /locus_tag="WGR19_000581"
     CDS             11872..13266
                     /locus_tag="WGR19_000581"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0798158.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMEWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13287..13682
                     /locus_tag="WGR19_000582"
     CDS             13287..13682
                     /locus_tag="WGR19_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798159.1"
                     /translation="MPSDVGIRLQTASKRKLNMKKILYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13686..14177
                     /locus_tag="WGR19_000583"
     CDS             13686..14177
                     /locus_tag="WGR19_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0798160.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMKSFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            14222..14332
                     /locus_tag="WGR19_000584"
                     /pseudo
     CDS             14222..14332
                     /locus_tag="WGR19_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(14406..17000)
                     /gene="mutS"
                     /locus_tag="WGR19_000585"
     CDS             complement(14406..17000)
                     /gene="mutS"
                     /locus_tag="WGR19_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0798161.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLAALRDSLFALSEIDLSANGSSLLETLKAVFP
                     ETLPVAETLKVAVMPEPAVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPKF
                     ADYPAIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDELNVGNFVDKAEEKHFEGILATALENLDPDRLTPREALSELYRL
                     KDLCKSVS"
     gene            17445..18449
                     /gene="gap"
                     /locus_tag="WGR19_000586"
     CDS             17445..18449
                     /gene="gap"
                     /locus_tag="WGR19_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0798162.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18519..18638)
                     /locus_tag="WGR19_000587"
                     /pseudo
     CDS             complement(18519..18638)
                     /locus_tag="WGR19_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18707..19387)
                     /gene="tsaA"
                     /locus_tag="WGR19_000588"
     CDS             complement(18707..19387)
                     /gene="tsaA"
                     /locus_tag="WGR19_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0798163.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLASAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDSTPIIDIKPYIPFVESKPDASSGFVSGKPEELEVV
                     WLENIGAENLSESTQNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19502..20173
                     /locus_tag="WGR19_000589"
     CDS             19502..20173
                     /locus_tag="WGR19_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0798164.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIVGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKNTLIINKLKLFH
                     VKHTS"
     gene            20177..21145
                     /gene="waaC"
                     /locus_tag="WGR19_000590"
     CDS             20177..21145
                     /gene="waaC"
                     /locus_tag="WGR19_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0798165.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPGVELHWLCEAGFTDI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            21371..22120
                     /locus_tag="WGR19_000591"
     CDS             21371..22120
                     /locus_tag="WGR19_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0798166.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22131..23066
                     /locus_tag="WGR19_000592"
     CDS             22131..23066
                     /locus_tag="WGR19_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0798167.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVTAAAKLGKVDLLVAGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            23129..23740
                     /locus_tag="WGR19_000593"
     CDS             23129..23740
                     /locus_tag="WGR19_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0798168.1"
                     /translation="MIATYKTITSLTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKGATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(23792..24109)
                     /locus_tag="WGR19_000594"
     CDS             complement(23792..24109)
                     /locus_tag="WGR19_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0798169.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24267..25538
                     /gene="purD"
                     /locus_tag="WGR19_000595"
     CDS             24267..25538
                     /gene="purD"
                     /locus_tag="WGR19_000595"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0798170.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
BASE COUNT         6095 a         5913 c         7255 g         6341 t
ORIGIN      
        1 gtcattccca cgaaagtggg aatccagaat ctcggacttt cagataacct ttgaatattg
       61 ctgttgttct aaggtctgga ttcccgcctg cgcgggaatg acggcggagc ggtttctgtt
      121 ttttccgata aattcctaaa actcaaaatt tcatcattcc cactttcatg ggaatgacgg
      181 gatgtaggtt cgtgggaatg acgcggtaca ggttttcgtt acgtatcccc atccgaaagt
      241 ttatttatac acaagcaatc caaagttatc tgactgaaat attgtgagat ttacccgcaa
      301 atctcatttt ttccaccact tatacacaaa ttcggatgcg cactctgccg atttgcagaa
      361 ataagacatt cgtgccgaca cctgaccatt tccacacgtt ccaaacacgc ttccggctta
      421 aatctccaca gtattcccgt tcttaaaggc gccagcagcc attctgtaaa gaaaagtagg
      481 atgccatctg cacaacaagc aaactatcca caaaacttat tccccataaa aaacaatcca
      541 caggtatgca caaaagggaa tccgccttac cgacacccta aaaaaaagaa atcctgatga
      601 tttttaaaat aaaaaaaagt tacccacaga aaatttctcc tatcaatata atcatcgttt
      661 tattatttaa attttatatt ttatttataa cccttcagca aaacggtaca gcaaagcgta
      721 aaggcatctt ttcaaaaaat taaaaagtta aggattgtta aacaagtgta aatcgggtag
      781 agttgcggtc gataaattaa atctatgacg gagcttccta caatgaaagg cgatattggc
      841 gtaatcggtt tggcggttat ggggcaaaac ctgattttga atatgaacga ttgcggattt
      901 aaggttgtcg cctacaaccg aacaatcggt aaggtggacg aatttttaaa cggcgcggct
      961 aagggaacgg atattgtcgg cgcgtattcc ctgcaagact tggtcgataa gctggcaaaa
     1021 ccgagaaaaa tcatgatgat ggttcgcgca ggttcggtgg ttgacgactt tatcgaacaa
     1081 ctgcttccgt tgttggaaga aggcgacatc attattgacg gcggcaatgc caattactcc
     1141 gacacgacac ggcggacaca ttaccttgcc gaaaaaggga ttttgtttgt cggggcgggc
     1201 gtatccggcg gcgaagaagg cgcgcggcac ggcccgtcca ttatgccggg cggggataag
     1261 cgcgcttggg aagccgtcaa accgattttt caggcgattg ccgccaaaac gccgcagggc
     1321 gaaccgtgtt gtgactgggt cggcagggac ggcgcgggac attttgtcaa aatggtgcat
     1381 aacggtatcg aatacggcga tatgcagttg atttgcgaag cgtaccagtt tatgaaagac
     1441 ggtttgggac tgtcctacga cgaaatgcac cgcgtgttta ccgcgtggaa caaaaccgag
     1501 ctggattctt atctgattga aatcacagcg gcaattttgg gctataaaga cgaaggcggc
     1561 gaaccgctgg tcgagaaaat cctcgatacg gcagggcaaa aaggcacggg caaatggacg
     1621 ggcatcaatg ctttggattt gggtattccg ttgacgctga tttccgaagc ggtattcgcc
     1681 cgctgcgttt cgtcgttcaa agaacagcgc gtgcagaccg gcaagctgtt cgcccgaacc
     1741 gttacgcccg tcgaaggcgg caaacaagaa tgggttgagg cgttgagaca ggccctgctt
     1801 gcatccaaaa tcatttccta cgcacaaggc tttatgctga tccgcgaagc gggcgaaagc
     1861 tacggctggg atttggacta cggcaacact gcgctgctgt ggcgcgaggg gtgcatcatt
     1921 cgcagcgcat tcttaagcaa tatccgcgat gcctatgaaa acaatcccga tttggtgttc
     1981 ttgggtacgg atccgtattt caaaaatatt ttggaaaact gcctgccggc gtggcgcaag
     2041 gtggttgcca aggcggtcga atgcggcatc cctatgccct gcatggcttc ggcgattacg
     2101 ttcttggacg gctacacgac cgaacgtctg cctgccaacc tcttgcaggc acagcgcgac
     2161 tacttcggcg cgcacactta cgaacgcacc gacaaaccgc gcggcgagtt tttccatacc
     2221 aactggacgg gcaagggcgg agataccgct tcgacaacct acgatatttg attccgttgc
     2281 cgaaatgccg tccgaaccgt tcggacggca tttgttttgc aggaaataga aagatgttcc
     2341 aatggcttta tgatgtattg tggctgcttg cgccgatatg gatacggcgt tatttggaca
     2401 aacgctccgg aagtgcccct gcatatcggg cgcatcggga cgagcgtttc ggcaagccgt
     2461 atccgaatcc cgttaccgga gcggtttgga tacacgccgt ttcggtcggg gaaacgcgtg
     2521 ccgcccaacc tttgatacgc gagttgcggc agcgttttcc cgatgcgccg ctgctgatga
     2581 cgcagatgac cccgacggga cgggaaaccg cgcaagttct gtttcccgat gcgcaatgcc
     2641 gttatcttcc gtatgacaaa aaaacgtggg tacggcagtt tttgcgcgaa caccgcccga
     2701 tgttcggcat tttgatggaa accgaaatct ggcccaacct gatgacggag tgccggaaat
     2761 cgggcgttcc gctgtttttg gcgaatgcgc ggctgtcgga aaaatcgcta aacggttatc
     2821 tgaaagtccg ccgcctgatc cgtcctgccg ccgcttcgct gacgggttgt ctggcgcaga
     2881 cggaggcgga tgcggcgcgg ttggcgaaat tgggcgcggc atccgtgcag gtgtgcggca
     2941 ataccaaata cgacctgatg ccgtcggaac agatgaaaac gctggcgggg cagtttgaaa
     3001 aacgcatcgg aggccggccg gttgccgtgt gcggcagcac gcgcgtttat cggggcgaag
     3061 acgaggcgga aaaactgctg gcggcgtggc aacaatatcg cggcgatgcg ctgctggtcg
     3121 tcgtgccgcg ccatcccgag cattttcaga cggtatttga aacggcaaaa cgcttcgggt
     3181 ttaaggttca gcggcgcagc gacggtttgc cggtcgaacc tgatacgcag gtgtggatag
     3241 gcgacagtat gggcgagctg tatgcgtatt acctgtgcgc cgatgttgct tttgtcggcg
     3301 gcagtctggt cgattcgggc tgtcagaaca tcatcgaacc gctttcctgc ggcgttccga
     3361 cgatattcgg cttttcgacc tacaattttt ccgaagcctg ccggcacgcc ttggcatcgg
     3421 gggcggcggt tcgggttgag tcggcggacg cgtggcggga agccgttgaa aaaaccttat
     3481 cgtccgaggg gggcggaatg cagatgcagg cgcacgtgga cggctttatc gcacaacatc
     3541 gcggagcggg cgcgagaatc gccgaggcgg tacgggaagc ggtatgcgga catcggggac
     3601 gatagtgata caatccgtat ccgagttttc cggttggagc atcgtatgag tatttatgcc
     3661 gtcgcgcaca tcgtccacct gtattgcgcc attgcctttg tcggcggcgt gttttttgaa
     3721 gtgctggttt tgtccgtcct gcatacggga cgggtgtcgt gcgaggcgcg gcgcgaagtg
     3781 gaaaaggcaa tgtcttaccg cgccgtcagg gtgatgccgt ttgtggtcgg actgctgttc
     3841 gccagcggca tcgtgatggc ggcaaaccgc tatctttcta tattgggcga accgtttgcc
     3901 acttccttcg gtacgatgct gacgctgaaa atcctgttgg cgttcagcgt gttggcgcac
     3961 ttcgccatcg ccgtcgtcaa aatggcgcgt tccacactga ccgtcggctg gtcgaaatac
     4021 atacacaccg tcgtctttac ccatatgctg ctgattgtct ttttggcaaa agcgatgttt
     4081 tatatcagct ggtaacacaa ctacatttca aatcaggaga ttgaaccatg acagaatatc
     4141 ccggcatcgg gtcgccgttg ttttacggcg ttttttttgc ggcagtgctg gtcatgattg
     4201 ccttggatat gttttcgctg aagaaaaaca gcagccataa agtcggcgtc aaagaagcct
     4261 tggcatggag cggcttgtgg gttgccgtat cctgcctgtt cgcaggctgg ctgtatttca
     4321 aactcgccgg caatcccggc tacggcgcgg cagtcgcaaa agaaaaagta ttggaattct
     4381 ttaccggcta cattttggaa aaatcgttgg cagtcgataa tattttcgtg ttcctgatga
     4441 tattcggcta cttcaaagtc gcaccgcagt ttcagcaccg cgtgctgctg tacggcgtat
     4501 tgggcgcatt ggtattgcgc accgtcatga ttttcgtcgg cgcggcactg gttcggcagt
     4561 tcgagtggat tctgtatctg ttcggcgcgt tcctgctcta taccggcata cacatgatga
     4621 agcccgaggg cgatgaggaa gaggatttgg caaacagcag gctgctgaat gccgtcaaga
     4681 aagtcgttcc ggtcggcacg gaatttcacg gcgagaaatt ttttaccgtc gaaaacggca
     4741 aaaaaatcgc cacgccgctg tttttggtgc tggtcatgat tgaattgagc gatgtcgtgt
     4801 ttgctgtgga cagtattccc gccgtctttg ccgttaccac cgatccgttt atcgtgctga
     4861 cttccaatat tttcgctatt ttgggtttgc gggcgatgta tttcctactg gcggatgtgg
     4921 cggaacgctt tatcttcctg aaatacggct tggcattcgt gttgagtttt atcggtgtga
     4981 aaatgctggt aatgcattgg gtgcatatcc cgatttccgt ttcactgtcg atcgtcttcg
     5041 gcgcgttggg tgcatcgata ctgacctcgt taatttatac gaaaaaacag tccgataaat
     5101 aaaatgccgt atgagcgccg acaaaatggt tgacagcatc aggcttgatg tatagaattt
     5161 cgagcttatc gggtcgttag ctcagtcggt agagcagcgg acttttaatc cgttggtcga
     5221 gcgttcgaat cgctcacgac ccaccagata aaacaaaaaa agctgagttt tcaaaactcg
     5281 gctttttttg tctgccgaca atccttgttc ggaaagcgcg gcatttcgtc aaattctttg
     5341 gtgcggcttc cgtatttttc ccgaaaatcc cttataattc cgcctctgta accttcatcg
     5401 ggtgatgctt tatgcgttgt cccgagtatt gaaatattac aagtacccac caggaaagaa
     5461 caaacgtgga caaactgaaa atctccgcaa acggcccgct caacggggaa ataacggtct
     5521 cgggcgcgaa aaacgcggca ttgccgctga tgtgcgcggg tttgctgaca tcgggtacgt
     5581 tgcgcctgaa aaacgtccct atgctggcag atgtgaaaac cacgcaaaag ctgcttcagg
     5641 ggatgggcgc gcgcgtcctg actgacaata tcagcgaatt tgaaatcaac ggcggcacgg
     5701 taaacaatac ctgcgcccct tacgaattgg ttcggacgat gcgtgcttcg attttggtgc
     5761 ttggtccgac gctggcgcgt ttcggcgagg cgcaagtcag cctgccgggc ggctgcgcca
     5821 tcggttcgcg ccccgtcgat cagcatttga aaggcttgga agcgatgggt gcggaaatcg
     5881 tcatcgaaca cggttacgtc aaggccaaag gcaaactcaa aggtacgcgc gtggcgatgg
     5941 atgtcgttac cgtcggcggc acggaaaacc tgctgatggc ggcgacgctg gcggaaggta
     6001 cgacggtttt ggaaaactgc gccatcgagc ctgaagtggt cgatttggcg gaatgcctgg
     6061 tcaaaatggg cgcgaaaatc agcggcatcg gtacgtccac gatggttgtg gaaggctcgg
     6121 acgagctgca cggctgcgaa cacagcgtcg tccccgaccg gattgaagca gggacgttcc
     6181 tgtgcgcggt ggcgataacc ggcggcaggg tggttttgcg gaatgccgcg ccgaaaacga
     6241 tggaagtggt gttggacaaa ctggttgagg caggtgcggt gattgaggcg ggcgacgatt
     6301 ggatcgccat cgatatgcgg cagcgtccga aggcggtgga catccgtacg gtcgtccacc
     6361 ccggcttccc cacggatatg caggcgcagt ttatggcatt gaatgccgtg gcggagggaa
     6421 gctgccgcgt ggtggaaacg atttttgaaa accgctttat gcacgtcccc gagttgaacc
     6481 ggatgggggc gaacatcaca accgagggca atacggcatt tgtgcagggt gtggaacagc
     6541 tttccggcgc ggtcgtcaag gcgacggatt tgcgtgcgtc cgcaagcctc gttatcgccg
     6601 gtttggcggc gcgaggcgaa accgtggtcg aacagattta ccacttggat cgcggttatg
     6661 aacatattga aaaaaaactc ggcagcgtcg gcgcgaaaat cgagcgcgta tccggctgat
     6721 cgggttgcga tgccgtctga agccttcaga cggcatcaat tgtttccgta tcgcaaacac
     6781 tgattgaaaa ggactgatta tggcattttt aaaactgacc gaacaaaacg tgcagggcaa
     6841 aaccgtcctc atccgcgccg atatgaacgt gccgttcaaa gacggcaaaa tcagcgacga
     6901 cacccgtatc cgcgcctcgc tcgcgtccat ccaatactgc atggacaacg gcgcatccgt
     6961 tatcgtgatg acccacttgg gccgcccgac cgaaggcgag ttccaccccg aagacgacgt
     7021 tgcgcccgtt gccgcgcatt tgggcggcct gttgggcaaa aacgtgaaag tattgaacga
     7081 ctggcgcgaa aacaaaccgg ctttgaacgc gggcgatgtc gtgatgctgc aaaacgtgcg
     7141 tatcaacaaa ggcgagaaga aaaacgattt ggaactgggc aaagcctacg cttctttgtg
     7201 cgacgtgttc gtcaatgacg cgttcggcac cgcgcaccgc gcccaagcct cgaccgaagc
     7261 cgtcgcccaa gccgctcccg ttgcctgcgc cggcgtattg atggcgggcg aactcgacgc
     7321 tttgggcaaa gccttgaaac agcccgcgcg cccgatggtt gccatcgttg ccggcagcaa
     7381 agtgtccacc aagctgacca tcctcgaatc gctggcggac aaagtcgacc aactcatcgt
     7441 cggcggcggc attgccaaca ccttcctgtt ggcggaaggc aaagccatcg gcaaatcttt
     7501 ggcggaacac gatttggtgg aagaatccaa aaaaattatg gcaaaaatgg cggccaaagg
     7561 cggttctgtg ccgctgccga ccgatgtcgt cgttgccaaa gcctttaccg ccgatgccga
     7621 agcggtcgtg aaagacattg ccgacgttgc cgaagacgat atgattttgg acatcggccc
     7681 gaaatccgcc gccgagcttg ccgatttgct caaagctgcc ggtacggttg tatggaacgg
     7741 ccctgtcggc gtgtttgagt tcgatcaatt cgcaggcggc acgaaagctc ttgccgaagc
     7801 cattgcccaa agcaaagcat tctcgattgc aggcggtggc gacacgctgg cggcgattgc
     7861 taaattcggc gttaccgacc aaatcggcta tatttctacc ggcggaggcg cgtttcttga
     7921 gttcttggaa ggtaaagagt tgcctgccgt agccgctttg gaaaaacgcg gcgcgtaagc
     7981 ggtttgatat aaaaaatgcc gtctgaaacg ttgtgctgtt tctgacggca tttttgtgtg
     8041 gcgattattg tgctttggct gcccattccg ccggagtggc ggcaaccgaa atggaaagtg
     8101 cgtgcagttc gttactttcc agttgggctt tgagtccgtc tttaatcagg cggtggcgcg
     8161 cgaggcgtgc cttgccttca aattctgatg aaacgatgac ggcgaaaaaa tggtgtccgt
     8221 cgccttctac ttcgatatgt tcgcattttg ccacgccttc aatcatggct tttacttgtt
     8281 ccgaggtcag catggtaggt tccttgttgg aaaaatgcgc cattatactg gcaaattgaa
     8341 atgccgtctg aaaacgggtt cggacggcat ttttgacggt gttttatttt ttggctttga
     8401 agccgagcag gtgctgggtg gtggcaagcc acgcgccgaa tacgcccaac gcgatgacga
     8461 agccgaacac cagcgcgatt tcttcaggat aaaagaaacg ccagccgata ttgagcccgt
     8521 agggtttgaa gatggcatcg accaaggggc gcactgtgga aagcagccaa ccgcaaagcc
     8581 ccaggctgac ggcggcggaa aggatgctct gccacattgc ctgataaagg aaggggcggc
     8641 ggatgaacga tgcgggcgcg cccaagagtt tggtgatttc gatttcttct ttgcggctga
     8701 ggatttgcag gcggatggtg ttgtgtgcga caaggacgaa cgccataccc agcgtcaggg
     8761 aaagaaacca taaaatttta cggataaatt cgttgatttg gtacagcgtt tgcacccatt
     8821 cggtatccat agacgcggat tcgaccatag gcagtttggt aatgtcccgg tagattgcct
     8881 gcatttgcgc gggcgttgtc gcggggtcgg gggtaacgat aaatgcgtcg ggcagcgggt
     8941 tgccgtcgag catcgaaacc aaattttggt cgaggttgga ctgtaattcc gccaagccgt
     9001 cttccttgcc gatgaagcgg acgttggcga tccgtttgtc gcgcgccagc aggctgcgga
     9061 cggtatcgct gtcgctttgt gcggcggcgg tttccatata gacggtgatt tgcggcgact
     9121 cgttgagttt gcccaacacg ctttgcccgc tttgaatgcc caaatacata aacagcggca
     9181 gggtcatcgc gacggcgagc atcaggaggg tgagcagcgt gccgaagggt tggcgcagaa
     9241 gctctttgag cgcggtgcgg gcggattcga tgtgcagcga ggcgtagtgg atgatgctca
     9301 tgcgagtcgt cctttcgaga ggcgcaggat gcggtgtccg tagtccgcca tcagggtttc
     9361 gtcatgggcg gcaacgatga cggttgttcc cgcttcgtgg aaggttttga acaattccat
     9421 gatgtcgagc gcgtaggcgc ggtcgaggtt ggcggagggt tcgtcggcaa tcagcaggcc
     9481 gggctggtga acgacggcgc gggcgatgca caggcgttgt tgttcaccgc cggagagggt
     9541 tacgggatcg tccaattctc gtcctttcag gccgactttt tcgatggcga tgcgggcgcg
     9601 ttcttcggct ttgcgcggcg gatagccgat aatccgaagc ggcaggatga cgttttgcag
     9661 gacgttgcgg tcgtagagga ttttgtggtc ttggaacacg atgccgatgt gttggcgcat
     9721 aaagccgatt tggttgtcgg tcagtgtgcc gaggtcttgg ttgttgaagt ggactttgcc
     9781 cctgcttggt ttggtaatgc ccgaaatcag cttgaggacg gtggatttgc ctgaaccgga
     9841 gtgtcctgcg ataaaaatca tctcgccttt gttgatttgg aagctgacgt ttttcagggc
     9901 ttcaaaaccg ccgggatagg ttttggaaac ttgttcgaaa cggatcatag gaggtcctgt
     9961 aaggaatggg cggcatatcg tttcgggcgg ttgcgtccgc agggatatgc cgtctgaaca
    10021 ataggtaaga cagtgccgat tataaagatt ccgcctgttt ttagatagtg gataagtttg
    10081 gcggataccg aaggtcgggc gggaaaaatg ccgtctgaag cggcttcaga cggcatccgg
    10141 cgtttaacgg cattttgaat gggcgagttt gacggcttcg gtcaggcttt tttcgttcac
    10201 ttccccggta atggtttgtt tgtacccgca tttcggggct tcgacgacgg taaagggcag
    10261 tacgccgaca ttgtttccgt aggatttcat aaagtttcgg ctgttcgcct cggtgtaacg
    10321 ccaaatcggg taggaaacag gagtttgttt gaggaagttg ccgatattgt cggatgtgtc
    10381 gagcgcgatg ccgaccatat cgacgctgcc ttttttctgc gctttgtacc atttggacat
    10441 ggcgggcatc tcttttcggc acgggccgca ccaggttgcc caaaggttga cgatgcgtac
    10501 gggggctttg agtgattgca ggctttgtgg ggtgttgtct ttccatccgg ccagttcgtc
    10561 tgccgaggcg gtatgtgcgg caaatgtggc ggcaagggtg attgcggcaa gattcaagta
    10621 tttcatcgtt ttgtcctttg tatgtcgatt cgggtattgt aaaccgtttg gtacggttgt
    10681 aggcgggata ttgagtaaaa ccctgtgtaa gccgataata aggggattgt cgagaagcaa
    10741 ggaaatgtca tgcctgaaat caaaattttc cacaatccgc gttgcagcaa atcgcgtgcc
    10801 gccttgtccc tattggaaga acgcggcatt gctgccgaag tggtcaaata tttggatacg
    10861 ccgcccgact tgtccgaatt gaaggatatt ttcaacaaat tgggcttggc atcggcgcgc
    10921 gggatgatgc gcgtgaaaga tgatttgtat aaggaattgg gtttggacaa ccccaatttg
    10981 gacaatgacg cgctgctgcg tgccatcgcc gatcatcccg ccctgttgga gcgtccgatt
    11041 gttttggcaa acggtaaggc ggctgtcggc agaccgctgg aaaatatcga agcggtattg
    11101 tgaacaaacg gctgttttgc agccgcaacg gcttgcgcta ttacctgttg ggcggtttct
    11161 gtttgagtgt ttttccgttt ttgctggtgt ttgcctcatc cgtttgggcg gtttatcgga
    11221 cgggcgggca ggttttgccg ccgtatgtcc gggcggatgc cgcgctggtg ttgggtgccg
    11281 ccgcgtggga caaacaccct tctccggttt ttcgggaacg catcaaccac gccatcgcgc
    11341 tttatcagag ccgcagggta ggcaaaatcg tgtttacggg aggacggacc aaaaaaggct
    11401 atatgaccga agccgaggtc gggcggcggt acgcgctcaa acagggggtt ccggcgcgca
    11461 acatcctgtt tgaaaacact tcgcgcaaca cttatgaaaa cctgaacaac atccgtccgg
    11521 ttttgcgtgc caacggtatt gccagtgtgg tgattgtcag cgatccctac catttggcgc
    11581 gggcggcgga aatggcggag gatttgggtg tccgcgttta tatgtctgcc acgccgacca
    11641 cgcgctttga tgcgggaaat aagaaaaaaa tctttatgct gcaagaggga tatgcgttat
    11701 ccctctaccg tttggaaaaa tggggcagcc gctttttggg atggctgtct gattgaagcg
    11761 gtagaaaatg ccgtctgaaa cttatccgca ggcagggttc ggacggcatt tttaatgccc
    11821 aagcacggta aaaacagtaa aataagccgt tttaaagatt ctggagccat catgaccgtc
    11881 aaaacccgtt tcgcccccag ccctaccggc tacctgcaca ttggcggcgt tcgcaccgcc
    11941 ttgttttcgt gggcgtttgc ccgccatcat aaaggcgaat ttttattgcg tatcgaagac
    12001 accgacttgg cgcgttccac cgccgaatct gtcaatatca tccttgacgg tatggaatgg
    12061 gtcggtctcg attacgacaa cgccgacaac gtggtgtacc aaacccgccg cttcgaccgt
    12121 tataaagaag tcatcgccga actcttggca aaaggcgatg cctactactg ctattgcagc
    12181 aaagaagagc tggaagcgat gcgcgagaaa gccgaaaaag aaggcacggc gacttacgac
    12241 cgccgctggc ggccggaagc aggcaaaacc ctgcccgaaa tccctgccgg cgtgcaaccc
    12301 gtcgtccgct tcaaaacgcc tttggatggc gtaaccaagt ggacggactt ggtcaaaggc
    12361 gaaatctcca tccccaacga agcactcgac gacctgatta tcgcccgcgc cgacggcacg
    12421 ccgacctaca acttctgcgt tgtcgtggac gactacgaca tgggcgttac ccacgttatc
    12481 cggggcgacg accatgtgaa caacaccccg aaacaaatca acatcttaaa agccatcggc
    12541 gctaccctgc ctgaatacgg ccacctgccg atgattctta acgaacaagg caaaaaaatc
    12601 tccaaacgca gcggcgatac cgttgccatt accgatttcg gcgcgatggg catcctgccc
    12661 gaagccatgc tcaactatct ggcacgcttg ggctgggcgc acggcgacga cgagttcttt
    12721 acgatggaac aattcatcga atggtttgat ttgaaagacg tttccccgtc cccaagccgt
    12781 atggatttga aaaagctcta ttggatcaac ggcgaacaca tcaaaatcac gcccaacggc
    12841 aaactcgccg aactcgtcaa accgcgtctc gctctgcgcg atatccatga aactgaaaaa
    12901 cctgcattgg aagacgtgtt ggcactggtc aaagaccgcg cccaagacct gaacacactt
    12961 gccgatgagt gcctctactt ctacgtcaaa caaacgccta ctgaagcgga cgtgcagaaa
    13021 cactgggacg atgaagccgc cgcccgtatg ctgcgctttg ccgaacgcct cgaagggctg
    13081 gaagactgga atgccgaagc catccacgac ctcttcaaac ccttctgcga cgaagaaggc
    13141 attaaaatgg gcaaactcgg catgcccctg cgccttgccg tctgcggtac ggcgaaaacc
    13201 cccagcgtcg atgctgtgtt ggcattaatc ggcaaagaag aagtgttgaa acgcatccgc
    13261 gcttaagtta tccacaggcg caggcgatgc cgtctgatgt cggaatacgg cttcagacgg
    13321 catccaaacg gaagttgaac atgaaaaaaa tattgtattt tctgatggtt gttttttcta
    13381 caagcgtatg ggccggggat gcggaagaca atctgctcag catccaatcc ggctatcgtg
    13441 ccttactgca aaagcaaaac aatctggacg gaaaaatcat cgggatgcag tcggatttgg
    13501 aagatgcgcg cagacggttg caggcggctc aggcagacat cgcccgtttg gaagcggaaa
    13561 ttcctgcagc aatggcgcaa aaagcccggc aggctgaaga gctgaggcaa atcggagtgc
    13621 gtttggacca tgcttggaat gcggtttacg gcgcaggggg aacgaaggcg tcggggaatt
    13681 gaccgatgaa ttccctcttt gtggacaata ctgttttcat tacacggctg aaagccgggc
    13741 atatcggcag gttggttcag gcgttgtttg aggagtggca cggatttgaa ccgtggtctt
    13801 ctgtggataa gattcgtgcc tattacggca gatgtttgaa agatgacgaa ctgccgctgg
    13861 cttttgcggc tgtggatgat ttcggaaccc tgttgggttc ggctgcggtc aagcggcatg
    13921 atatgaaaag tttcccacag tatgaatatt ggttggggga tgtctttgtt ttacctgaat
    13981 atcgcggaaa aggcattggc aggaggctgg tcgcccattg cataggcgca gcgcgttcgc
    14041 tggggataaa gttcttgtat ctttatacgc ctgatgtgca aatattttat gaatcattcg
    14101 gctgggcggt tgtcgggcga catttccata acggtgaatg ggttacggtt atgcgtttgg
    14161 atatggataa ggtttaagcc gaatggacaa atgccgtctg agctgttcgg acggcatttt
    14221 ttatagtgga ttaaatttaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
    14281 acggcaaggc gaggcaacgc cgtactggtt taaatttaat ccactatatg tttggaaatt
    14341 tcctgtcaga taatgtttat ccacagattt ttccatatgg tttgatgctg ttccgacaac
    14401 ggaaattaag atacggattt gcacaaatct ttcagacggt acagttctga caatgcttcg
    14461 cgcggagtca gtctgtcggg atcgagattt tccaaggctg ttgccagtat accttcaaaa
    14521 tgtttttcct ctgctttgtc cacaaagttg cccacattca gttcatctcc tttttcagac
    14581 ggcatggtac tgaaaatatc cagttgggga cggttcgcgg cggcttggtt ttccagttcg
    14641 ttcaaatgct tttgggcgga tttcaatgcg cgtacaggca gtccggcgag tttggcgacg
    14701 gcgatgccgt agcttttgcc ggcgggaccc ggttggattt ggtgcaggaa cacgatgtcc
    14761 cgtccctgtt cgagcgcgga aaggtgcata ttgagtgcgg cggcgtgggc ttcgggcagg
    14821 taggtcagct cgaaatagtg ggtggcaaac aggctgaagg atttgttttt ttgcagcagg
    14881 tgttcggcaa cggcgtgcgc gagggcgagg ccgtcgaaag tggaagtacc acgtccgact
    14941 tcgtccatta aaacaaggct ttgttcggtg gcgtgatgca ggatgtaggc ggtttcgctc
    15001 atttcgacca tgaaagtgga gcggttggag gcgaggtcgt ccgatgcgcc gatgcgggtg
    15061 aagatttgat cgatgggccc gattgtggcg gcatcggcag gcacaaaaca gccggtgtgt
    15121 gccaataaaa caatcagcgc gacttggcgc atgtaggtgg atttgccgcc catattgggg
    15181 ccggtgagca gcatgaggcg gtgtttgtgg tcaaggtcgg tgtggttggc ggtgaagtgg
    15241 cgtacctgct gttcgacaac gggatggcgg ccgttttcga tgtggatagc cggatagtcg
    15301 gcaaacttgg ggcggacgaa gttccgctct tttgccaagg ctgaaaatgt ggacaacacg
    15361 tccagcgcgg cggcggcttt ggcggctttt tgaagctgcg gcaatgccgt ctgaaggttt
    15421 ttcaatacgc cgtcaaagag ttgtttttct aaggcgaggg cttgctcttg agcagtcagc
    15481 actttgtctt caaaggcttt caactccggc gtgatgaagc gttcggcgtt tttgagggtt
    15541 tgccggcgtt ggtagtcggc aggtgcttgt tcggcttggg ttttggataa ttcaatgtaa
    15601 aagccgtgaa cgcggttgaa ctcgactttg agtgtggaca aaccggtacg ttcgcgttcc
    15661 ttggcttcca aatccagcaa aaattcgtcg ccatggtttt gaatgcggcg caattcgtcc
    15721 agttcgggat gaaaaccgtg gttgatgaca ttgccgtctt tcagccagac ggcaggttcg
    15781 ggcatcacgg cgactttgag ggtttcggca actggcaggg tttcggggaa aacggctttg
    15841 agggtttcca acaggctgct gccgtttgcg gacaagtcga tttcggacag ggcaaacagg
    15901 ctgtcgcgca gggcggcgag gtcgcgcggg cgggcgttgc cgacggcgat gcgggcagca
    15961 atgcgttcaa tgtccgcaat gtttttcaga cggcattgga ggggtttgta ttggctttcc
    16021 agcgcggcaa cggcttcttg gcgcgctcgg atgtgggcgc ggttgcgtaa agggtggtgc
    16081 agccagagag ccaagaggcg gctgcccata tgggtagcgc aaaggtcgag cgtggacatc
    16141 agggtcggcg attttttgcc ggagagggtt tgcgtgattt cgagattgcg gcgcgtggcg
    16201 gcatccatac cgatatattg gctgtcggtt tcgagcgaca ggccgtccaa atgttgcggc
    16261 atcaggtttt gcgtcaggcg gatatagttc aacagtgcac ctgccgcgcc aatcgcaacg
    16321 gcgtgttctt tgccgtccaa accgaagccg cgcaaatcct ggcagccgaa atattccgtc
    16381 agcagttttt cccccgcgtc ggcggcaaac tgccacgcgt tcaggcgcgt aacacccgat
    16441 gctgtctgaa gttgcggtgc gtttttactg tccggcagca ggatttccgc cgcctgcagg
    16501 cgcgccagtt cgtcgttcaa cttatccaca gttgtcagct tggttttgaa ttcgccgctt
    16561 tgcagcgatg cccacgccaa accgatgtat tttttgtcgg gggacacggc aacgatgcgg
    16621 ttggtttcct tgtcttccag caatgcggaa tcggtcagcg tgccgggcgt tacgatgcgc
    16681 acgactttgc gctccaccgg tcctttgcct gcgccgactt cgccgacctg ttcgcaaatc
    16741 gccacgcttt tgcccaactt gaccaggcgc gccagatatt gttcggcggc gtgaaacggc
    16801 acgcctgcca ttttgaccgg ctcgccatcc acctgtccgc gcgtggtcag ggtaatatcc
    16861 aaaagttttg ccgcttctac cgcatcgtcg aaaaacatct cgtaaaaatc acccatgcgg
    16921 taaaacacca gtttgtcggt atgttgcgct ttgatgccga ggtattgctg catcattggg
    16981 gaaacggcgg atttggacat ggggctgcct tatcattagg aaaacggtat tgtagcaaaa
    17041 ggttttgtct tcgacggaat gagactgacc gtaagcctgt caataaggtt gtggagacaa
    17101 gtaggaaaac agcaaagtta tccacggaga tattattaag tcaaaaaaac atccacgttt
    17161 atctgtccta tacggcaagc cttaaactca aacttatttg acgaaaaggt aatgaaaata
    17221 ttggatattt gcaacttata cacaagcaga tccgcaatca tcaaaatcat cattaaaatt
    17281 ttaaattcaa atattgatgg taaaagtttt ggaaatctgt gcaaacagta aaattcatct
    17341 atatcaaata agcatcaagt agttagatgt aatagtggaa tgtaacttta tttcagaata
    17401 caatacagat attgaaattt cgtttcacag cttggagtat atacatgagc attaaagtag
    17461 cgattaacgg tttcggccgc atcggccgcc tcgcattgcg tcaaattgaa aaagcccacg
    17521 gcatcgaagt cgtcgctgtc aacgacctga cccctgccga aatgctgctg caccttttca
    17581 aatacgacag cacgcaaggc cgtttccaag gtactgccga attaaaagac gatgccatcg
    17641 tggtaaacgg caaagaaatc aaagttttcg ccaatccgaa tcccgaagaa ttgccttggg
    17701 gcgagttggg tgtggatgtc gtcctcgaat gtaccggctt ctttaccaat aaaaccaaag
    17761 ccgaagcaca catcagggcc ggcgcgcgca aagtcgtgat ttccgcacct agcggcaatg
    17821 atgtgaaaac cgtcgtatat ggcgtaaacc aagacatttt ggacggcagc gaaaccgtta
    17881 tctccgctgc ttcctgcacc accaactgcc ttgccccgat ggcggcagtc ctgcaaaagg
    17941 aattcggcgt agtcgaaggc ctgatgacca ccatccacgc ctacaccggc gaccaaaaca
    18001 cccttgacgc gccgcaccgc aaaggcgatt tgcgccgcgc ccgcgccgcc gcgctcaaca
    18061 tcgtgcccaa cagcaccggt gccgccaaag ccatcggtct ggttatccct gaattgaacg
    18121 gcaaactcga cggctccgcc caacgcgtcc ctgttgccac cggctcgctg accgaattgg
    18181 tttccgtcct cgaacgccct gtaaccaaag aagaaatcaa cgccgcgatg aaagcagccg
    18241 ccagcgaatc ttacggctac aacgaagatc aaatcgtttc ttccgacgtt gtcggcatcg
    18301 aatacggctc gctcttcgat gcgacccaaa cccgcgtgat gaccgtgggc ggcaaacaac
    18361 tggtgaaaac cgttgcctgg tacgacaacg aaatgtccta tacctgccaa ctcgtccgca
    18421 ctttggaata ctttgcaggc aaaatctaag gcttgaacaa acctgtggat aagtaaatgc
    18481 cgtctgaaca cattgcgttc agacggcatt gtttatctgt atagtggatt aaatttaaac
    18541 cagtacggcg ttaccttgcc ttgccgtact atttgtactg tctgcggctt cgttgccttg
    18601 tcctgattta aatttaatcc gctataactt gatattgcgg cttatggcga acaaacaaca
    18661 gccgtttgtt caactgctat gcggcaaaac cggatttttg cccgatttaa agcggggttg
    18721 gggaaagatc aataacggtt gcacggtttt cttcgatttg aaacctaact tcgtaatccg
    18781 caatattcat cacataaatc cgttcgggaa tatcctgata ggcggggcgc gggtcttggg
    18841 caatgctttg gctgattagg ttttgggtgc tttcagataa attttccgcg ccgatgtttt
    18901 ccagccatac aacttcaagc tcttcgggtt tgccgctgac gaagccggat gatgcatcgg
    18961 gtttggattc gacaaagggg atgtagggtt ttatgtcgat aattggtgtg ctgtccaaca
    19021 ggtctgcgcc gctgcaatag aggcggacgg gtttgccggt ttcgatgcgt tcgagtttca
    19081 ggagcgagag tccgagatgg ttggggcggt gggggctgcg cgtggcgaac acgcccattt
    19141 tttgtttgcc gccgagccgt ggcgggcgca ccatttgcgc ccagccttca tccaatacgc
    19201 cgtgaaaaat aaaacttatc cacacataat cgaaatcttc cagcccgcgc acgctgtctg
    19261 cggtaaattc gggattcagc tcgatgcata cttctgcggc agaggctaaa ccgggctggc
    19321 gggcgatgcc gaatttctgt ttgtagggcg atcgggcggt gccgatgggg gtaatggtat
    19381 aagtcataag gttgtggata agtttttttg gaaaaacctt atcttatcac aatcggtttt
    19441 accgtttttg ggcagtaatt tgttataata cagggcattt taatcgcgtt gaaataatga
    19501 gatgattgtt tccattgatg ttgatgcaca aaaaacgttt acgcctttgt gtcctaaaga
    19561 gctgcctgtg aacgaggggc atttgattgt tgaagagttg aatgcccaag ccgctttggc
    19621 ggatttgcgc gtgatgacga aagatgcgca tcatgcggca gcaaaatggc ttgtggataa
    19681 ccctgttgat atgttgaagc cgacaggttt gcctgatgcg gatttgactt gggtggctca
    19741 tgcgatggtc ggtacgcgcg gctatgaatt attggacgga ctgccttctg ccaaagaata
    19801 cgattattgc gtttggaaag gtgttgatcc tgaattgcat ccttacggcg cgtgttttca
    19861 cgatattgag gaaaaactga gcacagggct gattgaatgg ctgcgttgtc aaaatacgga
    19921 tacggtcatt gtcggcggct tggctacgga ttattgtgtg aaaacaacgg ttttgcagtt
    19981 gcttaaaggt ggtaattggc aggttatcgt caatgaagcg gcttgtcggg gtattgcgcc
    20041 ggataccatc gaaacggcat ggcaggaaat gcgttctgcc ggtgctgtca tcttaaaaaa
    20101 cgccgaagaa attaaaaaaa acacattaat aatcaataag ttaaaattgt ttcacgtgaa
    20161 acatacttct taaaatatga aaattttgct tgtccgtttg tctagtatgg gcgatttaat
    20221 tcacactttg cccgcaattg aggatttggc gcgacaatgc cctggtgtag aactccattg
    20281 gctgtgtgaa gctggattta cagatattgc gcgcctgcat ccgtttgtga aaaaaatcca
    20341 tgtgatgaaa tggcggcaat ggcgcaaaca tctctttcag gctgaaactt ggcgggaaat
    20401 gggtcatctg aaacagactt tgcggcagga agcatttaat tttgtattgg acagtcaagg
    20461 tctgattaaa agcgcgtgtt tcgctaaaat ggcgaaatcc cctatttatg gtttggataa
    20521 aaatagcgcg cgcgagggac tggctgcgct ggcgtatgcg aaaacatacg ctgtaccgaa
    20581 agggaaaaat gctgtttggc gcaatcgtga gctgttcgcc caagtatttg ggtatgcgat
    20641 gcctgaaacg caggtatttg gcttgactgt tcctgaggca ggccgtctga aaaatttagc
    20701 gcagccgtat tatgcggctt tgcatgcgac cagtcgggac agtaagttat ggcctgtgga
    20761 aaactggcgg tcgttgctgc aaaagttgaa tgaagaacag caatgcaata tttacctgcc
    20821 ttggggaaat gaagatgaaa aaacgcgtgc caaacagatt gcagacggac tgccgtttgc
    20881 tattgtgtgc gacaaaataa atttattgca ggcagcgtat ctgctgaaat acgcggtcgg
    20941 aattgtcggc gtggataccg gtttgctgca tttggcaaat gccttggaaa aacctgtggt
    21001 cggcatttat accgataccg atccgattaa aacaggcgtt caggtttcgc caattgcaaa
    21061 aaatttgggc aatatcgggc agattccgac cgcagatttg gtttatcaaa cgttgatgga
    21121 ttgtgttgca gcagacaaag gctaaagggt tgtctgaaac tgatatagca cctgatattt
    21181 ttcagtttgt taagcataag aatgtatatc taccagcctg agtaaatgct ttaaaaatgg
    21241 tataatcggc aagcattatc cgaaagattt ttgaatgaat tcgtttttaa ggaaaacgga
    21301 tttggtttta tagggaagaa ggtttggttt cgctggatgg tgaaagcagc gttcttttta
    21361 ggagggttca atgaaagcac tggtcgcagt aaagcgcgta gtggactaca acgtcaaagt
    21421 tcgtgtaaaa gccgatggtt cggatgtgga tatcggcaat gtcaaaatgt cgatgaatcc
    21481 gtttgacgaa atcgctgtgg aagaagccgt ccgtttgaaa gaagccggaa aagtaagcga
    21541 gatcgtagcg gtttctttgg gtgaaaagaa atgcgaagaa accttgcgta cagctttggc
    21601 gatgggtgcc gaccgtgcaa ttcatgttga aaccgatgcg aaattggagc cgctggcagt
    21661 tgccaagttg ctgaaagccg ttgcggacaa agaaaatccg caaattttct ttttgggtaa
    21721 acaagcgatt gatgacgatg ctaatcaagt ggcgcaaatg ctggcagctt tgttgaatgc
    21781 ggcgcaaggt acgtttgctt caaaagtaca aattgaaggt gatgaagtac agattgtgcg
    21841 cgaaattgat ggcggcgaag aaaccatagc attgaaattg cccgctgtta tcagtgcaga
    21901 tttgcgtttg aacgagccgc gcttcgtcaa actccccaat attatggcgg cgaagaaaaa
    21961 acctttggaa aaactggctc ctgccgattt ggttgctgac atttcacctc gtctgaaaac
    22021 ggtgaaattc gccgagccta aagcgcgtca ggcaggcgta aaagtagcaa gcgttgccga
    22081 attagttgag aaattgaaaa acgaagccaa agtgatttga ggagactgaa atgagcgtat
    22141 tgattattgc cgaacacgac aacaaacagt tgaatcctgc cactttgcat gctgttaccg
    22201 ctgccgcaaa actaggcaaa gtcgatttat tggttgccgg aaacggcgca tcgtccgtcg
    22261 tggagttcgc gaagcaagta gcgggagtgg aaaaagtttt ggttgcagat gctgcccatt
    22321 atgctgaagg tttggctgaa gagttggctc cgctggttgt caaattggcg gcggattacc
    22381 gctatgttgc agcaacggca accacattcg gtaaaaacct tttgccccgc gtagccgcct
    22441 tattagacgt accgcaaatt tctgatttga ccgagattgt agataacacg acttttgtac
    22501 gtccgattta tgcaggtaat gcatttgaaa ccgtgcaagc caatgcggaa aaattggtgc
    22561 tgaccttccg tgcgacggct tttgacgcag tggcggcgca aggtggtaat gctgaggtaa
    22621 ttaatgttga agcaacccct gcccaaaacc tgagtcgttt tgttaaccgt cagctttccc
    22681 attccgatcg tcctgaactg actcaggcaa aagtgattgt ttccggtggt cgtgcgttgg
    22741 gtagtgcgga aaaattcaat gaagtgctga caccgttggc ggatgtttta ggtgcggcaa
    22801 tcggtgcatc ccgtgcagca gttgatgccg aatatgcgcc aaacgatgct caagtcggac
    22861 aaaccggtaa agtggttgcg ccgcaactct attttgcaat cggtatttca ggtgcgattc
    22921 aacacgttgc cggtatgcaa gacagtaagg tgattgttgc aatcaataaa gatgctgatg
    22981 cgccgatttt caatgtagcc gattacggat tggttggcga tttatttgaa atcgttccac
    23041 aattaacaga agcattgaaa aattgatgtt tcacgtgaaa cgttcagacg accttgttat
    23101 taatgaggtc gtctgaaaac atttcagcat gattgcgact tataaaacca ttacctcctt
    23161 gacgcaggct gagtttaaag ataaaggcag ccgttttatt gcatttgcct atccgattcg
    23221 gacattggct gatgtgaaaa aatacctcga tccgctaaag gaagagcatc ataaagcacg
    23281 acactggtgc tatgcctatc gtttgggtgt ggatggtgtg caatttcgtg ccaacgatga
    23341 tggagagcca tcaggaagtg ctggacgacc aattttggga cagattgatt cggtgggtat
    23401 taccgacgtt ttggtcgtgg tcgtccgcta tttcggtggt actttgttag gtgttccagg
    23461 tctgatacat gcgtacaaag gggcgacggc tcaagcgttg gcagttgcag aagtagttga
    23521 aaagaatatt gaaaaaactg tttggctgaa atgtgaatat ccgtttttga atgaagcgat
    23581 acgcattgcc aaacaatatc aggcggatat attggagcgg gatttgcagt tggattgccg
    23641 attgacggtc agtttgtcgt tggtaaatta tgaggcctgt gttgcggctt ggaagaatac
    23701 acggcagatt gaagtaaata cggaaaagcc ttttgaatga aaggtcgtct gaaatgcttt
    23761 gtgatggtat tttagacgac ctcttcgctg actaatcttc ttgtgtatat ttgtctaagg
    23821 catcgacgct ggagcgcaaa tataaccaag ccaattgatg gaattcgccc agcgcgttcc
    23881 atagcgcatc ttcactcata atgctgtatt cgccttccgt acgcaaatcc agttctaccc
    23941 cgtcttcaat agcctgatga cacgcctgat actcgtgggt atagaaattg tccatgtcgc
    24001 gaattaaagt gacgacatat ttccaacggc ggatgtcttc ttccagttgc ggtagtaaat
    24061 gacaccattc gcggtatttg ctttggattt catcaatacg tttttgcatg gttgcttgtc
    24121 cttttttaat ttatggataa gttgaatagg ttgcagagtg gaatgaagcg gtcttttgtt
    24181 tgattctact aaaatttctt cagatgggat aaaagtccct ttgtatcgag ttttcgttaa
    24241 aagtacatat ttgtcaagga gatggtatga aactgctggt tatcggtaat ggcggtcgcg
    24301 aacacgcgct ggcttggaaa ttggcgcagt cgcctaaagt ggaaacagta tttgttgcgc
    24361 ctggtaatgc cggtacagcg attgaaccca agttgcaaaa catcgacttg actgcgcatc
    24421 aggatttgat tgaattcagt cgtaaagaaa atattgtttt taccgtcgtc ggccctgaag
    24481 cgcctttggc ggcaggtgtt gtggatgatt tccgtgccgc aggactaaaa atattcggtc
    24541 cgacacaata tgcggcgcag ttggaaagtt ccaaagattt cgccaaagca tttatggcga
    24601 aatacaacat tccgaccgcg caatatcaaa cctttgaaaa cgccgatgcc gcacatgatt
    24661 acgtcaatca gaaaggtgcg cctatcgtta tcaaagccga tggtttggcg gcaggtaaag
    24721 gcgtgattgt ggcgatgact ttggatgaag cgcatgctgc gattgatgat atgctgctgg
    24781 acaacaaaat gggcaatgcc ggcgcgcgtg ttgtgattga agatttcctg caaggcgaag
    24841 aagcgagttt tatcgtcatg gttgatggca ataatgtact gcctatggca accagccaag
    24901 accacaagcg tcttttagac ggcgacaaag gtctgaatac gggcggtatg ggtgcgtaca
    24961 gccctgcgcc tgtggtaacg cctgttgtgt acgagcgcgc gatgaatgaa attattttgc
    25021 cgaccgtagc agggatgaaa gcggaggggc atgagttcac cggtttcctg tacgcaggtt
    25081 tgatgattga tcaaagcggt gcaccctata cgattgagtt taactgccgt ttcggcgatc
    25141 ctgaaaccca gccgattatg agccgactga acagcgactt atcggatttg gttgaagcgg
    25201 caatagacgg cagactcgat agcgtaaccg cggaatggag cccgcaaact gccgtgggcg
    25261 tggtactggc ggcgcaaaat taccccgaaa cgcccaaaaa aggcgatgtt atttccggtc
    25321 tggatgctgc caaccaagta ggcaaagttt tccatgcagg tacaacggca aacgagaaag
    25381 gtgacgtatt gaccaacggc ggacgcgtat tgtgtgttgt gggattgggc gacaatgtgg
    25441 cgcaagcgaa agctaaggca tatggcgcgt tggaaaaaat cagtttcgac gggatgcaat
    25501 accgtaaaga cattgccgat aaagcgatta accgttaact agaaagagga tgatgccgtc
    25561 tgaagccaag tatgactgat gtgcttcaga cggtatttta tatt
//
LOCUS       DAWFJW010000017        25033 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFJW010000017 DAWFJW010000000
VERSION     DAWFJW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25033
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.017"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..830
                     /locus_tag="WGR19_000596"
     CDS             <1..830
                     /locus_tag="WGR19_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0798171.1"
                     /translation="QVALVVTESTGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQP
                     LTKTDAKDAKMPAFFAQMTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQPADMRTAEK
                     NRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHTHTHFDGKAQVAEQIKGIGSITT
                     ATLMAMLPELGRLSHKRIAGLVGIAPHPRESGETKFKSRCFGGRSAVRKALYMATVAA
                     TRFEPLIRDFYQRLPSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
     gene            878..1951
                     /locus_tag="WGR19_000597"
     CDS             878..1951
                     /locus_tag="WGR19_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0798172.1"
                     /translation="MRPFCVGATVLAILGALVFFISPGVVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPATTLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAW
                     LIWLARNTDNFALLMLLAAFTVFQTAYAATGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAVLMNVNPIFFITVPAILTAT
                     VFVLYLLTFVPIFRANAFTGDPE"
     gene            complement(1997..4186)
                     /locus_tag="WGR19_000598"
     CDS             complement(1997..4186)
                     /locus_tag="WGR19_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0798173.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILGVQTTFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAVQGWIETTEAAKPVLRPYHGQASHSEFVLNANQQKASDAIQTAFGSFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVDRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPHEA"
     gene            4325..5107
                     /locus_tag="WGR19_000599"
     CDS             4325..5107
                     /locus_tag="WGR19_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0798174.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5521..7455)
                     /locus_tag="WGR19_000600"
     CDS             complement(5521..7455)
                     /locus_tag="WGR19_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0798175.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7521..8699)
                     /locus_tag="WGR19_000601"
     CDS             complement(7521..8699)
                     /locus_tag="WGR19_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0798176.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQASYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8925..9314
                     /locus_tag="WGR19_000602"
     CDS             8925..9314
                     /locus_tag="WGR19_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0798177.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVK
                     STLLMNAQTMERYYRQKGTFDNYDKNKLKQNEYFDITLSEVSPDHFTLKATPNPTTND
                     GETCVVTLNDGGTFSAAGNGQSCPDFD"
     gene            9503..10549
                     /gene="adhP"
                     /locus_tag="WGR19_000603"
     CDS             9503..10549
                     /gene="adhP"
                     /locus_tag="WGR19_000603"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0798178.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(10714..14199)
                     /gene="smc"
                     /locus_tag="WGR19_000604"
     CDS             complement(10714..14199)
                     /gene="smc"
                     /locus_tag="WGR19_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0798179.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQVTGHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPYGFVPPTPLPQGQAAWL
                     SDGLSSGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVMLYAKPAQESLIAQKARLDGIASELESLAPELSAAEAAFKQAE
                     VTVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIAILQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            14201..14452
                     /locus_tag="WGR19_000605"
     CDS             14201..14452
                     /locus_tag="WGR19_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798180.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            14449..15171
                     /gene="lpxH"
                     /locus_tag="WGR19_000606"
     CDS             14449..15171
                     /gene="lpxH"
                     /locus_tag="WGR19_000606"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0798181.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            15440..17026
                     /locus_tag="WGR19_000607"
     CDS             15440..17026
                     /locus_tag="WGR19_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0798182.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVTAMIFFL"
     gene            complement(17194..17397)
                     /locus_tag="WGR19_000608"
     CDS             complement(17194..17397)
                     /locus_tag="WGR19_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0798183.1"
                     /translation="MDNLHMREIAVSPENLPLYCSGPDNEEWNGHPRVFLPLREGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17383..17559
                     /locus_tag="WGR19_000609"
     CDS             17383..17559
                     /locus_tag="WGR19_000609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798184.1"
                     /translation="MKIVHLIFLCVVGVRPFYAIGRLKDIAEAAEQDLPPPTEHIRSN
                     RETFAHMHGCFQPV"
     gene            17616..18809
                     /locus_tag="WGR19_000610"
     CDS             17616..18809
                     /locus_tag="WGR19_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0798185.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19213..20148)
                     /gene="hemC"
                     /locus_tag="WGR19_000611"
     CDS             complement(19213..20148)
                     /gene="hemC"
                     /locus_tag="WGR19_000611"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0798186.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVILQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20267..20782
                     /gene="ybeY"
                     /locus_tag="WGR19_000612"
     CDS             20267..20782
                     /gene="ybeY"
                     /locus_tag="WGR19_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0798187.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KSGYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            20784..21608
                     /locus_tag="WGR19_000613"
     CDS             20784..21608
                     /locus_tag="WGR19_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0798188.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(21751..23130)
                     /gene="nhaC"
                     /locus_tag="WGR19_000614"
     CDS             complement(21751..23130)
                     /gene="nhaC"
                     /locus_tag="WGR19_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0798189.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23421..24642)
                     /locus_tag="WGR19_000615"
                     /pseudo
     CDS             complement(23421..24642)
                     /locus_tag="WGR19_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
BASE COUNT         5712 a         7109 c         6522 g         5690 t
ORIGIN      
        1 ctcaggtcgc cctcgtcgtg acggaaagta ctggcggtct cgaaatcccc gccgccaaag
       61 ccatccgccg agcaggcata gccgtgatta tcgccaaccc gcgtcagacg catcagtttg
      121 cccaatcgca gccgctgacc aaaaccgacg ccaaagatgc caaaatgccc gccttcttcg
      181 cacagatgac ggcacagaaa gaagattcgc aaaccatgcc ctaccaaccg cccaccgaag
      241 cggaagaagt gttggaagcc ttggttaacc gccgcaacca accggcggat atgcggactg
      301 ccgagaaaaa ccgtctgcat caggttcacg aaacgcaagt cggaagcgtc aaacaactga
      361 ttgcccattt tgaccggctg attgacgaat tggacaaaca aatcgacgac cacacccaca
      421 cgcattttga cggcaaagcc caagtggcag aacaaatcaa aggcatcggt tcgataacga
      481 cggctacgct gatggcaatg ctacccgaat tggggcggct gtcgcacaaa cggatagcgg
      541 gtttggtcgg cattgccccg cacccgaggg agagcgggga aaccaaattc aaaagccgct
      601 gctttggcgg aaggtctgcg gtgcgtaagg cactgtatat ggctaccgtg gcagcgacac
      661 gttttgaacc gcttattcgg gatttctacc aacgcctgcc gtccgagggt aagccgtata
      721 aggttgccgt tacggcatgt atgcgcaaac tgctgacgat atcgaatgcc cggatgcgtg
      781 attattttgc cgaaaacgat accgccgaaa acggtatccg gacggcttga tttgagtttt
      841 ggtatttttg cccgacgggg tgaaaaatac tgttgctatg cgcccgtttt gcgtcggcgc
      901 gacagtgctt gccatactcg gcgcgttggt gtttttcatc agccccggtg tcgtcgtcct
      961 gcaccgccaa atcttcttag aactcatgct gcctgcagca tatggtggtt tcctgactgc
     1021 ggctttgttg gactggacgg gtttttcggg caacctgaaa cctgccacta ctttgatggc
     1081 ggtgttgttg cttgttgcgg ctgttttatt gccgttttta ccgcaacttg ccgcattctt
     1141 tgtagcggct tattggctgg ttttattgct gttctgcgct tggttgattt ggctggcccg
     1201 caacacggat aacttcgccc tgctgatgct gcttgccgcg ttcaccgttt ttcagacggc
     1261 atacgcggca accggcgact tgaacctgtt gcgcgcgcaa gtgcatctaa atatggcggc
     1321 ggtgatgttc gtatccgtgc gcgtcagtat tcttttgggc gcggaagccc tgaaagaatg
     1381 ccgtctgaaa gaccctgttt ttattccaaa tatcgtttat aaaaacatcg ccattacttt
     1441 cctgctcttg cacgccgccg ccgaactttg gctgcccgcg caaaccgccg gttttaccgc
     1501 gctcgccgtc ggcttcatcc tgctcgccaa gctgcgtgag cttcaccatc acgaactctt
     1561 acgtaaacac tacgtccgca cttattacct gctccagctc tttgccgccg caggctattt
     1621 gtggacaggc gcggcgaaat tacaaaacct gcccgcctcc gcgcccctgc acctgattac
     1681 cctcggcggc atgatgggcg gcgtgatgat ggtgtggctg accgccggac tgtggcacag
     1741 cggctttacc aaactcgact acccaaaact ctgccgcatc gccgtcccca tcctcttcgc
     1801 cgccgccgtc tcgcgcgctg ttttaatgaa cgtgaacccg atattcttca tcactgtccc
     1861 cgcgattctg accgctaccg tgttcgtgct ttacctgctg acattcgtac ctatttttcg
     1921 ggcgaatgcg tttacgggcg accctgaata aaaatgccgt ctgaaacggt ttcagacggc
     1981 atcggattgc caataatcaa gcctcatgcg gatcgacatc caccgaccat cggattttgc
     2041 cgtccctgtt ttgctgcaac acctgcaccc acaaactcac ggcgcggtgc aaatcctgtc
     2101 gggatgttga ttcgaggaag atttgcgcgc gttcgcgttc ggcgaggcgc accatcagca
     2161 tcggggcggc gccgaactgt gaaacgcttt cgggcaaaag cggggcgagg gtttctttgg
     2221 cggcgttgag aaattccatc gcatcggcaa cacgcggcgc gtccgcgcgg acggccgtct
     2281 gaaaaccgaa gggcggcatg gcgaacattt gccgctcgtt caattcgttt tcggcaaaca
     2341 cggcgtagtc ctgcgctttg acggcggcga agacgggatg ttcgggcagt tgggtctgta
     2401 tcaacacctt gccgggtttg tcggcgcgcc ccgccctgcc ggacacctgc atcagctcgg
     2461 cgaacagcct ttccggggcg cgaaagtccg cgctgtacag gctgccgtca gcgttcaaca
     2521 cgataacgag gttgagccgc gcgaaatcat gccctttggc gagcatctgc gtgccgacca
     2581 aaatgtcgat tttgttgtcg gcgatgcggc ggtacaaatc cgcccagtcg tttttgtgcg
     2641 cggtgctgtc cctgtcaaca cggacgacgg ctgccttggg caggaaggcg cgcagggttt
     2701 cttcgacgcg ctgcgtgccg tggccgacgg cggtcaggtc ttggttaccg cagtcggggc
     2761 atttgaacgg tatgggttcg cggtggtcgc agtggtggca gcgcagttgg cgggcgcgtt
     2821 ggtgcagcac cattttggcg gagcagttcg ggcagccgaa ggtatgaccg cagtcgccgc
     2881 aaaacagcgc gggcgcgaag ccgcgacggt tgaggtacac cagcgacata ccgcccgctt
     2941 caaagttctg cttcaaaagt tgcaaggctt gcggcgagaa gccgttgtca agtttcagac
     3001 ggtctacgtt gaggatgtcc acttgcggca gttgcgcggc ggtatgggcg cgttcggtca
     3061 gttgcagcag gcggtacgcg ccgctttgcg ccttgtgcca gctctccaag ctgggggtgg
     3121 cactgcccaa tatgatcggg cagccgccct gcttcgcccg ccacaccgcc aaatcgcggg
     3181 cgtggtagcg caattcgttg tcctgtttga acgagccgtc gtgttcctca tcgaccacaa
     3241 tcagcccgac atcatccatc ggcgtgaaca ccgccagccg cgtgccgatg accaatttcg
     3301 cctgccccaa catcgcgcgc aaataatcct gcgtgcgctt gcctgccgcc atctgactgt
     3361 gcaacacggc ggtcggcacg tcggcaaaac ggttttccac ccgcttcaaa agctgcggcg
     3421 tgaggttgat ttcgggcaac agaaacaaca cctgccgccc ctgcgccaac actttcgcca
     3481 tcgcatcgaa atacacctcg gtcttgccgc tgccggtgat gccatacagc agaaacggtt
     3541 ggaagctgcc aaaggccgtc tgaatggcat cggaagcttt ctgctggttg gcattcaaca
     3601 caaattcaga gtgcgaagcc tgcccgtggt acggccttaa aacgggtttc gccgcttccg
     3661 tcgtttcaat ccaaccctgc accgcccaat cttcaatcaa tttcgccgcc tgcgcgttta
     3721 cctgcttcaa cgctgccatc gtcatttcgc ccgacagcag cgcgtcccac aaagccgctt
     3781 ttttgttgaa ccgcgccggc ggcggcgttt gcgccctgcc cgcttcgttc aacgcgtaaa
     3841 acaagggcgg ctgcggcatt tccaccgcgc gcgtttcctt caaaccttgc ggcaatgcgg
     3901 caaatactgc ctgcccggtc ggatagtggt aataacgcga cgtaaacgcc aacaaatcac
     3961 gccagctttc gggcagcagc ggttcgtccg aaaaggtcgt ctgaacgccc aaaatccgcg
     4021 ccgtatccat atccggcgca atatccgttt cccacaccat cccgaccacg gttttattgc
     4081 ggaaaggcac aagcacccgc gttcccggag gaagcggatc ggaatgggaa taagtcaaaa
     4141 ggccgtctga aagcggcacg tttacggcga tgcggtggta gatcatacag gcaggcaaat
     4201 catttttttg cgtattttaa caggcggaag ctattttcag acgataaatt gagcctaggc
     4261 gcaatgcagg cgcaacagag atttgataca attccgcttt tccaacacgc gcaatcatct
     4321 ttatatgaaa accaagttaa tcaaaatctt gacccccttt accgtcctcc cactgctggc
     4381 ttgcgggcaa acgcccgttt ccaatgccaa cgccgaaccc gccgtcaaag ccgagtccgc
     4441 cggcaaatcc gttgccgcct ctttgaaagc gcgtttggaa aaaacctatt ccgcccaaga
     4501 tttgaaagtg ttgagcgtca gcgaaacacc ggtcaaaggc atttacgaag tcgtcgtcag
     4561 cggcaggcag attatctaca ccgatgccga aggcggctat atgttcgtcg gcgaactcat
     4621 caacatcgac acgcgcaaaa acctgaccga agaacgcgcc gccgatttga acaaaatcga
     4681 cttcgcctcc ctgcctttgg acaaagccat caaagaagtg cgcggcaacg gcaagctgaa
     4741 agtcgccgtc ttctccgacc ccgattgtcc gttctgcaaa cgcttggaac acgagtttga
     4801 aaaaatgacc gacgtgacgg tttacagctt tatgatgccc attgccggcc tgcaccccga
     4861 tgccgcgcgc aaggcgcaaa tcttatggtg tcagcccgac cgcgccaaag cgtggacgga
     4921 ttggatgcgt aaaggcaaat tcccggtcgg cggcagcatc tgcgacaatc ccgtcgcgga
     4981 aaccacttcc ttgggcgaac aattcggctt caacggcacg ccgaccctcg tcttccccaa
     5041 cgggcgcagc caaagcggct acagcccgat gccccaactg gaggaaatca tccgcaaaaa
     5101 tcaataatcc atccgcaaaa aaggcttgga aaattccaag ccttttttgc gggaaatgcc
     5161 gcctgaaaac caatccgccc tgaaagttag caagccaaca aacacatcct aacccattga
     5221 atgattgaaa ataataaatt gaacaatacg aagccccgcc gccgttccca cgcgggcggg
     5281 aatctggaat attgagttac cgtgatttat ccgaaacaag caaagccgaa cgaaccgaat
     5341 tcccgtcttt acgggaatga cgggactcga gcgttgtgtg caatcatcga acccccgccg
     5401 ccgtcattcc cacgcaagtg ggaatctgga acgtagaatc taagaaaccg tttttgtcca
     5461 ataagtttct gctccaaaat gaccggtcct gcagcttcag actgcatctt tgttccaacc
     5521 tcaatcctgc gccaatgcgt ctatcggatt gagtttggct gctttattgg caggcataaa
     5581 gccgaacgcg atgccgattc cggtcgaaca ggcgaccgcg ccgatgacgg atgccgccga
     5641 aatgtccatc gggaagtcgg ttacaaaatg attgaacacg aggctgacgg cggcggacaa
     5701 acccacgccg accaaaccgc cgatgacgca gattaacacc gcctcaatca aaaactgctg
     5761 caaaatattg ccgcgccgcg cgccgattgc catccgtatg ccgatttctt tggtgcgctc
     5821 ggtaacggac accagcatga tgttcatcac gccgatgccg ccgactacca atgaaatcag
     5881 ggcgatggag gaaatcagca gcttcatcgt accggtggtg ctttcgacta tctgcctgat
     5941 gctgtcgctg ttgttcataa agaaatcttc cgtgccgtgc cgcgctttga gcagatcggt
     6001 cagccctttt tcggcaacct gggtattggc attgtctttg attttgacgg tgatggagtt
     6061 ggtgtggctc tcgcctgtga tttggtgcat caccgtcgta tagggcgacc aaagcatcag
     6121 cacgtcggaa ttgccgaaag cgttttcgtc ttttttcatc acgccgatga cggtcaaggg
     6181 gcgtttcctg aacaaaatgg ttttacccaa cggatccgag tccgcaaaga gtttgtcttt
     6241 gacattttgg tcgatgacga cgacctgcgc gtcttctttc acatcgtttt cgtcaaacag
     6301 ccgccccgtt tccagcttca gtccgcgcac gtcgaaatat tgttcgccca cgccgtacaa
     6361 agaagcggtc aggtcggtat tgcggtaagt cagcgtgccg ccgctcgaag tcatgggcgt
     6421 ggcggaagca acgtagcttt gtttggcgat gatttttgcg tcgtctatgg tcagggtttt
     6481 aatcctgccg ctgcgcctgt cgccgaagcc gcgccccggg aagatgctga tggtgttcgt
     6541 ccctatcgaa ctgatgtctt caaggatttt tttctgcgaa ccattgccca atgcgacgac
     6601 ggagaccacc gacgcgatac cgatgatgat gccgagcatc gtcagaagcg aacgcatttt
     6661 gtgcgccaat actgcttgca ccgacattct gaaggcttcg acaaactggt cgtaataaaa
     6721 cgaccacgag gctttttccc gaatcctccc gacattgctt gcggggattt cgggattttt
     6781 cgaggtgtcg gaaatgattt cgccgtcccg gatttcgatg acgcggttgg cattggcggc
     6841 gatgccgggg tcgtgcgtga ccataatgac ggtatgcccg gcttcgtgca gcttatggat
     6901 gatttccatc acgtttttgc cgctggcggt atcgagcgcg ccggtcggct cgtcggcgaa
     6961 gatgatttct ccgccgttca tcagggcgcg ggcgatggag acgcgctgct gctgtccgcc
     7021 cgagagttcg ccgggcttgt tgccctcttt gcttgccaaa cccaaatcct gcaagagttt
     7081 gtccgcccgc gcggaacgct ctttgccgcc cgcgcccata tagacggctg gcagcgcgac
     7141 gttgtccctt gcggtcagcg agcttaagag gttgtagcgt tggaagatga aaccgaagcg
     7201 ttcgcggcgc aatgccgcca gttcgtcagg ctgcattttg gcagtttcga tgccgtcgat
     7261 tcggtacgaa ccggaaccgg cggtatccaa acagccgagt atgttcatca gcgtggactt
     7321 gcccgaaccg gactgcccga tgatggcgac aaaatcgccc ttctctatcg acaggctgat
     7381 gtctttcaaa atatggacgc ggttctcgcc gctgccgaaa tagcggttga tgtttttaca
     7441 ttcgatcaag ctcataatat tccgtaaatc aataacaaat gccgtctgaa accgtttccg
     7501 tgttcagacg gcatattcgt ttatcggcgc ggcgggccgc ctagggcgcg ttcgccgctt
     7561 tcctgctgct cggcggcggt tatttcggag atgaccactt tgtccccctc tttcaacccg
     7621 ctttttactt cggtattcat actgtctctc ataccggtcc ggatttcgcg ttccgccgcc
     7681 ttgccgtccg cacccaacac gcgcacaaac gccttgccgc cgcgattttt cacggtcagc
     7741 gacggaataa tcagcacatt tttcacgccg tcgatttcaa ccgtattctg cgtcgtcatc
     7801 cccgtggcga gtttgccgtc cggattgggc acaaacgaac gggcataata gtagaccgca
     7861 ttggaagccg tatccgtact gctgttgtag ccgcccgacg acatcgtggt cagcccgggg
     7921 tcgacgctgt cgagcttcgc cttaatcggc gtatccggtt cggacaaaat cgtaaacgaa
     7981 atatcctgcc ccgccttcac cttggtaata tcgccctcgg caatctgcat tttgttcaac
     8041 atcatatcca gattcgccaa ttggacaatc gtcggcgtag actgcgccgc gttcacagtc
     8101 tgcccctctt ccacgagaat cgccaccacc gtgccgtcca tcgttgcggt aatgcgcgtg
     8161 tagcccaatt ccgactcggc ggtattgatg gaaattttgc tctgtctgat tagagccttc
     8221 agctcggcaa cattggcttt ggcggcggca aacgcatcct gcgcgctttc caaatcctct
     8281 ttggaagtcg cgttttcctt ccataacgcc gcctgacgct tatatttctt ctccgcgctg
     8341 cccaatgcaa tctgtgccga caccagcttc gcctgatacg tttccaactt ggatttttcc
     8401 gtattgagcg tattggtctg cgaggtcgaa ttgatttccg caatcaaatc gccctttttg
     8461 acctgttgcc cgagcttgac gtaaagtttc ttaatctgcc ccgacgcctg cgcgccgacc
     8521 gacaccaaat tcgacggcga aatctccccc gtcgcggaaa ccgtccggct gatgtcgccg
     8581 cgcctgaccg tttccgtaat ataagaagcc tgcggttcgg gcttcagata agaccatccg
     8641 ccccaaaccg ctgccgccgc gaccgccgca acagccgccc atttcatcat ttttgccatg
     8701 ttttcaattc tctgtatgcg tcaatcccat aaatcggaag cggattctac cgcaagtccc
     8761 tgccccatcc aaggcggcaa actgccgaca ataaaaataa ccatttgttt taaatggctt
     8821 atacaatctg ctttcccgaa aatttcaatc cgtttacata ttccgccatt tccgatacgg
     8881 cgttataatg ccgtcgaccg acaaacaaat ggaaacacat tgctatgaaa aacgttcaaa
     8941 aaggctttac gctgctcgag ctgatgattg ccgtcgccat cctcggcatc ctgacgctca
     9001 tcacctaccc ttcctataaa acctacatcc ggcgcgtccg cctgtcggaa gtcaaatcca
     9061 ccctgctgat gaacgcgcag accatggaac gttactaccg ccaaaaaggg acgtttgaca
     9121 actacgacaa aaacaaactg aaacaaaacg aatatttcga tatcacttta agcgaagtca
     9181 gccccgacca tttcaccctt aaggcaacgc ccaatcccac caccaacgac ggcgaaacct
     9241 gcgtcgtcac gctcaacgac ggcggcacat tttccgccgc cggcaacgga caatcctgcc
     9301 cggacttcga ctaatcctcc gccgaacccg catccggcaa ccgccccgat gctgtgcctc
     9361 cccaactgtt ataatataac atataaccca tatttttcaa caacatacca cttgacctgt
     9421 gagatgcgga agcgtatgct tacccttata tcggacaaca ttccgtttac aggttttttt
     9481 gccgtaacta aggagcgcaa tcatgaaaat gcaggcagtt gttgtgaata aaaatgtagc
     9541 gggcgatgtg gaaatagtcg aacgcgaggt tcgcccgttg gaatacggcg aggcgttggt
     9601 cgaagtcgaa tattgcggcg tgtgccacac cgacctgcac gttgcggcag gcgactacgg
     9661 cgaaaaaccg ggccgcgtgc tgggacacga gggcatcggt ttggttaaag aagttgccga
     9721 cggtgtgaaa aatctgaaag tcggcgaccg cgtcagcatc gcctggctgt tccaaagctg
     9781 cggctcgtgc gaatactgca ataccggccg cgaaaccctg tgccgctctg tattgaacgc
     9841 gggctacacc gccgacggcg gtatggcgac ctactgcatc gtgagtgccg attacgcggt
     9901 caaagtccct gaaggtttgg atcctgcgca agcttccagc attacttgtg ccggcgtgac
     9961 cacttataaa gccattaaag tttccagtgt tcgtccggga cagtggattg ccatctacgg
    10021 cgcgggcggt ttgggcaact tgggggtcca atacgcgaaa aaagtatttg gcgcacacgt
    10081 tgtcgccatc gacatcaacg acgacaaact ggcgtttgcc aaagaaaccg gcgcggattt
    10141 ggttgtcaac gccgccaaag aagacgctgc caaagtgatt caggaaaaaa ccggcggcgc
    10201 acacgctgcg gtcgtaaccg ccgtatctgc tgccgcattc aactctgccg tgaattgcgt
    10261 ccgcgcaggc ggacgtgtgg ttgccatcgg gctgccgccg gaatcgatgg atttgtccat
    10321 cccgcgtttg gttttggacg gcatcgaagt ggtcggctct ttggtcggca cgcgcaaaga
    10381 tttggaagaa gccttccaat tcggcgcgga aggtttggtt gtgccgaaag tccaactgcg
    10441 tgctttggat gaagcacccg ccattttcca agaaatgcgc gaaggcaaaa tcaccggccg
    10501 tatggtgatc gatatgaaaa aagaatgcgg ctgcggccat caccactgat ttgacgtggc
    10561 agtacacatc gaaatgccgt ctgaacgctg ttcagacggc attttttatg gatttggatt
    10621 tgattttaat ctgttctgtt tgaaataccg tctgaaaacc catatcgcaa cacttcatta
    10681 aaaacggcaa gattcagccg ttctgcaacc cgttcaaacg gcttccgcca tttccaacgc
    10741 ttgtttgatg tccacggcga cgacgcgcga cacgcctttt tcctgcatgg ttacaccgac
    10801 gagctgctct gccatttcca tggtcaggcg gttgtgggag atgtagagga attgggtttg
    10861 cgccgacatt tctttgacca ggttgcagaa acgtgaggtg ttggcatcgt ccagcggggc
    10921 atcgacttcg tccaaaaggc agaagggggc ggggttgagg ctgaacaggg cgaacacgag
    10981 gctcatggcg gtgagggctt tttcgccgcc ggaaaggagg tggatggtgc tgtttttctt
    11041 gccgggcgga cgcgccataa tggacacgcc ggcggtcaaa aggtcgtcgc ctatcatttt
    11101 gagggtggct tcgccgccgc cgaacagggt cgggaagaaa gtttggactt tgccgttgac
    11161 ggcatcgaag gtttctttga aacgcgcttt ggttttgtcg tcgatttggg cgatggcttc
    11221 ttccaaaagg gtgatggctg cctgcacgtc ttcgctttgg ctgcggtagt agccgtcgcg
    11281 ttcgcgcgct tcttcgagtt cttgcagggc ggcgaggttg accgcgccga gtgcttcgat
    11341 ttgttgcgaa aggctgccga tgctgctgtt caatactttc ggcgattctt tcgccaacgc
    11401 ttcgagcgcg tccaaatcgg cggcgcgttc ggtcaggttt tgatggtagc gtttggcgtt
    11461 gatcagggct tcctgctgct gcaacaaggc ggtttgggtg gcggcctgaa gctgcggcag
    11521 cttggtttgc agggtttgca ctttagcgta ttgctccctg ccctgttcct gaatctgcgc
    11581 gagtttctct tgcacaacaa tatattcttc gtccaaggtc tgtacggctt cgcttaattc
    11641 ttcaagcttg atgtgctgct cgtcgttttg gaactcggtt tcataggcga gggcaagctc
    11701 ttgctggcgt tcctgccagt ccagggtttg ctgttcaagc tgggcgattt gctgccggta
    11761 attttgtttt tgctggttga gcttgtggac ggcgacttcg gcaagcccgt attggcggtt
    11821 ggcttccaac agggcaagct gcgcctgttt cagacgacct tgctgctctt ggcggctgtg
    11881 cgcggcggtt tgctgctggt gttcgagttc ggcggcggct tcctgcaaaa tggcgatgtc
    11941 gtctgaaagc ccgtcggacg tgtgttgcaa cacggtctgc tcttccgcca actgcgccag
    12001 ttcgcgctcg atgtgttcgc ggcggatttg cccttggttg gtgcgcgcca gaagttcggc
    12061 ggcgcgttgc tgtgcttggc tgtattggcg cgtgtgctgt tgttgctgct gcatcaggtt
    12121 tttgtgttgc acttcggacg agcgcacggt gacttccgcc tgtttgaacg cggcttcggc
    12181 ggcggaaagt tcgggggcga gactttccag ttcggacgca atgccgtcga gacgcgcttt
    12241 ttgggcaatc aggctttcct gcgcgggttt ggcatagagc atgacgctga ctttgtcgac
    12301 ctgatggcct tcgggcgtga gccagatttg gtgtgcgccc aaatcgcttt gatgcgcgag
    12361 ggcgtagctc aaatcgggcg cgcacaatac gccgtcgagc cagtggtgca atgccgtctg
    12421 aaacggcggc tgcgcttgga tttggttcag caatgcctgt acgggcaggg attttttgat
    12481 gccgctggag aggccgtctg aaagccatgc cgcctgcccc tgcggcaaag gcgtgggggg
    12541 aacgaagccg tacggtacgg cgcgggcgtg caggcgttcg gcaaggatga cggacaaggc
    12601 gtgttgccac tcggcgggcg cggtgatgtg ttgccacagt tgcggcgcgg cggcgtggcc
    12661 ggttacctgc cagaaatcgg cggcttcctg ctgttgcgac aggatttgcg acaacgcctg
    12721 ctgctgcgcc tgcaaggtga tatgttgctg cttcaggctt tggaagcggt ttgatgccgt
    12781 ctgaaacgcc tcgcgggcgg cgtgtagagc ttcttcggcg gcgatgattt gttcttcgta
    12841 atgctcttgc tggctttgca acaaggcggc ggcttcctgc gcggcggcgg tttcggcttc
    12901 gtcgggcagg ttcagggctt ggttttcctg tttcagacga cctttgcgct cttcgtgctt
    12961 ggcaacggtt tgttcggcat gggcaagctg ctgctgcttc aacgccagtt cgcggcggat
    13021 gcggtttgcc tcgtcctgct gggtttggaa ggcggcattg agcgtggctt gggcttcttc
    13081 caattcgggc agacgctctt cgtgttcggc aacctgcatc gcccattccg ccagctcggt
    13141 ttgtttttct tcgacctgca actcgttttc ttcaagctgc acgcggattt gctgctgctc
    13201 ttgatgaatg cgttgtaact gcgcctgcgc cgcctgcttg tcgcgttcga tgcgttgatg
    13261 caggttttgc tgatggcgga tttgttcttc caaacgggca atctgctcgc gcaacacgcc
    13321 gcgtttgttg cccagttcgt gtaccgcctg ctgctgcgac tgttcggcgg tctgcaaggc
    13381 gtgtacttcg tcgtttaacg cctgaacctg cgcggcggtt tcgtcctgct gcgcctgcaa
    13441 agactgatgc tgcgcggtcg ctttgtcggc ggcggcaagc gattgctgcc attgggcgta
    13501 atcgagcaaa tcctgttgcc ggttcaactg cgcggtcagg gatttgtagc gttcggcggt
    13561 ttccgcctgt ttctccagct tttccacctg acgcgccaat tcgttctgca aatcgcccaa
    13621 acgctgcaaa tgctcgcgcg tgtctttcag acggccttcc gtctccttgc ggcgttcctt
    13681 atatttggac acacccgccg cctcctcgat ataagcgcgc aactcctccg gccgcgcttc
    13741 gatgatgcgc gaaatcatcc cctgctcgat aacggcataa ccgcgcgcgc ccacgcccgt
    13801 acccagaaac aaatcagtaa tatcgcggcg gcgcacggtc tgattgttga tgaaataagt
    13861 cgattcgccc tgccgcgtca gctgccgctt gatgctcacc tcggcatact gcccccacgc
    13921 gccctgcaaa ctgtggtcgc tgttgtcaaa caccagctcc accgaagccc tcggcgcagg
    13981 acggcgcgtc gccgcaccgt taaaaatcac gtcctgcata ctctcgccgc gaagctgctt
    14041 cgccgaagcc tcacccaaca cccagcgcac cgcgtcaatc acattcgact tgccgcaacc
    14101 gttcggcccg ataaccgcga caagctgccc cggcacatga attgtggtcg ggtcggtaaa
    14161 agatttgaag ccggagagtt tgatatgggt caggcgcata atggtcggaa aaaataaaaa
    14221 agaagcgtat tttaacggaa atcccgccgc accaccccat atcttgccgg caaaacctta
    14281 ccgcatcccg caccctcgat gccgtctgaa gccttcagac ggcattttta acgcgcccga
    14341 accccgattc cgccaacgcc cgatgcttgg cgcggcattt gccgtattgc cccgccgccg
    14401 ggcgcgttat aatccgccac gacatccgcc cgatttggaa acctgcctat gaaacccgcc
    14461 tatttcatct ccgacctgca tttgagcgaa aagcaccccg aactgaccgc gctgttgctg
    14521 cgttttttac gttcttccgc cgccgggcag gcgcgggcgg tttacatttt gggcgatttg
    14581 tttgattttt gggtgggcga tgacgaagtt tccgagttga atacttcggt tgcgcgtgaa
    14641 atcaggaaat tgtccgacaa aggcgttgcc gtgttcttcg tcaggggcaa ccgcgacttc
    14701 ctgattggtc aggatttttg ccggcaggca ggcatgacgc tgctgccgga ttactcggtt
    14761 ttggacttgt tcggctgcaa aaccctcatc tgccacggcg acactctgtg tacggatgac
    14821 agggcatacc aacgtttccg caaaatcgtg catcggaagc ggctgcaaaa actgttcctg
    14881 atgctgcccc tgaagtggcg cacgcgcctt gccgccaaaa tcaggcgcgt cagcaaaatg
    14941 gaaaaacagg tcaagcccgc cgatattatg gatgtcaatg ccgcctttac cgcgcggcag
    15001 gttcgcgcct ttaacgcgga aaggctgata cacggacaca cccaccgcga gcatatccat
    15061 cacgaaaacg gctttacccg catcgttttg ggcgactggc ataacgacta tgcttcaatc
    15121 ctccgcgtgg acggggacgg cgcggtattc gtgccgctgg aaaaatgctg aaaatgccgt
    15181 ctgaagcctt tcgggcggct ttttttacac gcccctttcc cacactccct ccgccgcatc
    15241 atcaccatcc ttaacccgca ttaatgggcg ttatcgataa aatgcttgac cgattacata
    15301 agattacgta aagtgtgcaa acgcataaca attggtctta ccaatttatc ttttaaccaa
    15361 aatatcgggt gtacgggatt tgaacgtctt tccaaacctc gacgcgcctt atgatgcaaa
    15421 tgcctaaaaa ggattcgtta tggcactttt cctcagcata ttccccatcg tcctgctgat
    15481 ttggctgatg gtgaaaaaaa acagtatgcc ctcctacgtc gcgctgccga ttaccgcagt
    15541 gctgatttac gccatcaaac ttttctactt cggcgatgcg ggcatgctgc tcaacgccac
    15601 cgccgcttcc ggcctcgtca aaacgctcac gccgattacc gtgattttcg gcgcgattat
    15661 gttcaaccgt atgatggaaa ccacgggctg cattgatgtc atccgcaaat ggctggcgac
    15721 catcagcccc aaccccgtag cgcaactgat gattatcggc tgggcttttg cctttatgat
    15781 tgaaggcgca tccggcttcg gcacgcctgc cgcgattgcc gcgccgattc tgatgagctt
    15841 gggcttcaac ccgttgaaag tggcgatttt cactttggtg atgaactccg tccccgtctc
    15901 tttcggcgcg gtcggtacgc cgacctggtt cggtttcgca ccgctgaacc tgagtgccga
    15961 agacatcctc gccatcggca ggcagaccgg cgtaatgcac ttcttcgcag gtttcgtcat
    16021 ccccgtcatc ggcttgggct tcatcgtacc ttggtctgaa atccgcaaaa acttgggctt
    16081 cgtcgccatt gccgtcttct cctgcaccat tccttatgtc gcattggcga tggtcaacga
    16141 agaattcccg tcgctcgtcg ccggcgcaat cggcctgatg gtgtccgtat tcgccgccaa
    16201 ccaaggctgg ggcttgagca aagaccacgc caaagacccg aatgccgaaa aagtgccgtt
    16261 cgcccaagtc gccaaagcac tcgccccttt gggtatgctg atcggcatgc tggtggttac
    16321 gcgcatcaaa cagctcggca tcaaaggcat tttgaccagc aaagaagaat ggttcagctt
    16381 ccaactgccg tttgatttgt ccaaaatcac cgtcagcgac tccctgacga ttaccttcgg
    16441 caatattttc ggacaagatg tcagcgcgtc ttaccaaacg ctgtacgtcc cggcttggat
    16501 tccgtttgtg ctgaccgttt ggatttgcat cctgctgtat aaaaccaaat tcaaagatgc
    16561 ctggacgatt tattccgtaa ctttcaatca aaccaaaaaa ccgctgcttg ccctgatggg
    16621 cgcgctgatt atggttcagc tgatgctggt cggcggcgac aattcgatgg tgaaaatcat
    16681 cggtaaggaa tttgccgcaa tggcgggcga acactgggtt tatttctcgc cgtatctggg
    16741 cgcgatcggt gcgttcttct ccggctccaa caccgtgtcc aacctgactt tcggcccgat
    16801 tcagcagcaa atcgccttgg ataccggcct gtccgttacc ctgattctgg cgttgcagtc
    16861 cgtcggcggc gcgatgggca atatggtgtg cctcaacaac atcatcgccg tatgtaccgt
    16921 attggacgtg aaaaattccg aaggtgcgat tatcaagaaa accgttatcc cgatggcgat
    16981 ttacggcgtg attgccgtcg tcacggcaat gattttcttc ctctaggcga aacgccgcca
    17041 tccatagcgg cagcacgcag ggaacggaaa ccgccgttcc ccgtccgggc ggtgcgtcgg
    17101 gcagaatctc ggtgccggaa cagcccgccc cgccaaacaa atgccgtctg aaaccggaaa
    17161 aggcttcaga cggcattttt ccgccgtttg cattcaggcg tagtgatgat gcggcatctt
    17221 gccgtcaagg cggtagcgtg tgccgcaata cgggcaggca acgctgcccg attctccttc
    17281 cctcaacggc aggaacaccc tcggatgccc gttccattcc tcattgtccg gaccggaaca
    17341 gtataaaggc aggttttcgg gggacacggc aatttcccgc atatgaagat tgtccatttg
    17401 attttccttt gcgtggttgg cgtgcgccca ttttacgcca tcggcaggct aaaggatatt
    17461 gccgaagccg ccgaacaaga tttacccccg cctaccgaac acatacggtc aaaccgggaa
    17521 accttcgccc atatgcacgg ctgtttccag cccgtgtaaa acagcgtaaa atcggcaaac
    17581 ccgaatgacc ttccgtcccc atcaaggagc aagctatgtt cttcaagcac atcgaagccg
    17641 cccccgccga tccgattctc ggtttaggcg aagcgttcaa agccgaaacc cgccccgaaa
    17701 aagtcaacct cggcatcggc gtttataaag acgcatccgg cgcgacaccc ctcgtcaaag
    17761 ccgtcaaaga agccgaaaaa cgcctgttgg aaagcgaaac caccaaaaac tacctcacca
    17821 tcgacggcgt tgccgactac aacgcgcaaa cccaaatcct gctgttcggc aaagaccacg
    17881 aaatcatcgc cagccgtcgc gccaaaacag cgcaaagcct cggcggtacg ggcgcattgc
    17941 gtattgcggc cgagtttgcc aaacgccagt tgaacgcgca aaccatctgg atttccaatc
    18001 cgacttggcc caaccacaac gccatcgcca aagcggtcgg tatccaagac aaaccttatc
    18061 gttactatga tgccgccaaa cacggtttgg attgggacgg catgattgag gacttgagcc
    18121 aagcgcaaaa aggcgacatc gtcctgctgc acggctgctg ccacaaccct accggcatcg
    18181 accctacgcc cgaacaatgg gaaactctgg caaaactttc tgccgaaaaa ggctggctgc
    18241 cgctgtttga ctttgcctac caaggcttcg gcaacggttt ggaagaagat gcctacggct
    18301 tgcgcgtgtt cttgaaacac aatacagaat tgctgattgc cagctcttat tccaaaaact
    18361 tcggtatgta caacgagcgc gtcggcgcgt tcactttggt ggccgaagat gaagaaacag
    18421 cagcccgcgc ccacagccaa gtcaaaacca tcatccgtac cttgtattcc aacccggctt
    18481 cacacggtgc gaacaccatt gcgctggtgt tgaaaaatga tgatttgaaa gcacaatgga
    18541 ttgccgaact cgatgaaatg cgcggccgca tcaaagccat gcgccaaaaa tttgtcgagt
    18601 tgctcaaagc caaaggtgca agccaaaact ttgatttcat tatcgaacaa aacggtatgt
    18661 tctctttcag cggattgact cccgaacaag tcgaccgttt gaaaaacgag tttgccattt
    18721 atgccgtccg ctccggccgc atcaacgttg ccggcattac cgacgacaac atcgattatc
    18781 tgtgcgaaag catcgtgaaa gtcttgtaaa acaaaagcgc atacgaaatg ccgtctgaac
    18841 cttaaccagt tcagacggca tctgtttttt gttaaaaaac aataaaccat aacaatttca
    18901 aacaagctaa aaaacatcta taaaatcaac ccaatatgat gaactcaaat aaaaaatacg
    18961 aatatcatca ttctcaaatt caaaatttga acgccacagt attatccgaa gccacaaaaa
    19021 cctttccgcc gtcattcccg cgcaggcggg aatccgggaa cccaatgctg caagaattga
    19081 ttagaaacaa ctgaaaccga gcaaactgga ttcccgcctg tgcgggaatg acgaaataag
    19141 gcgagtaaaa ctgatgaaac aaattgatta aaaacaataa cttatattat tttatttaaa
    19201 ttttcgataa aatcaatttt ccgtattcaa tactgctcca atcaattccc gcgcaccgtc
    19261 gtccgccaat ttcttagcga cagcgcgtcc gagcgcgtcg gcatattcgg caggggcttg
    19321 cgcgtccgcc tgcaaaatga ccgaaccgtc ggggtgtccg accagtccgc gcaaggtcag
    19381 caagccgttt tcttccgtgc aatatgcggc caaaggcact tggcagcttc cgcccaaagc
    19441 gcgcgccaaa gcgcgttcgg ccgtaacgca ggcgtgtgtg gtatcgtggt ttagcggttt
    19501 taaaacctca tacaaatctt cgcggtgcgc ggcaatttcg atacccaatg cgccttgtcc
    19561 ggcggcaggc aggctgtcgg attccgacaa aatcatgcgg atgcgtccgt ccaattccag
    19621 acgctgcaaa ccggcggcag ccaagataat tgcgtcgtat tcgccgttat cgagtttaga
    19681 caaacgggtt tgcacattgc cgcgcaaagg tttgataacc aaatgcggat agcgcgcacg
    19741 caactgggct tcgcggcgca ggctggatgt gcctacgacg gcgcctttgg gcatttcttc
    19801 caaacgcgcg tattggttgg acacaaacgc gtcaaacgga ttggcgcgtt cgccgatggc
    19861 tgcaagcgcg aaaccttcag gcaaatccat cggcacatct ttaatcgaat gcacggccaa
    19921 gtcagcgcgc ccgtcatata aagcctgttc caactctttg acaaacaagc ccttaccgcc
    19981 gacttttgac aaagttttgt ccaaaatctg gtcgccgcgc gtggtcatgc ccaaaatctc
    20041 gacttcgcaa tcgggataca gggctttcag acggccttgg atatgctttg cctgccacat
    20101 agcaagcagg ctttcgcggc tggcgatgac gagttttttc gggttcatgt tcaatgcgtt
    20161 caaagaatgg aaacggcgtg aagtctatca tattttgaac gtttcttcag acggcaccgt
    20221 cagatctatg ttatatttac acccacaaaa cacgaaaccc gccacaatga aacgcaccaa
    20281 aaaataccct ttcttaacgt tgcagcggca acgtttccat ttgaactttg aaaacgcctc
    20341 ttccgccgcc ggcatcccag ccgaacgcga tttctaccgc tgggcgtggt ctgccttgaa
    20401 aagcggatac cgccgcgccg acatcagcct gattcttctg gacgaagaag aagcccgagc
    20461 ctacaaccgc gactaccgcg gcaaagatta cgccaccaat gtattgagtt tcgcgctcaa
    20521 cgaaggcgaa atcctgccct gccaagtttc ggaaaaactg tacggcgatt tgattatctg
    20581 cccgcaagtg gttttgaaag aagccgccga acagggcaaa acgcccgaac ggcattttgc
    20641 ccacctgacc atacacggcg ttttgcacct gatgggctac gaccacatcg aagacgatga
    20701 agccgaaata atggaagccg aagaaatccg cctgatgctg gcggcaggct tccccaaccc
    20761 ctaccaagag gacgaatatt aaaatggacg gtacgcagtc gaaacccaag tttttcgaac
    20821 gcctgatctc ccgacttgcc ggcgagcccg actccgccga agacgtattg accctgctgc
    20881 gccaagcgca cgagcaggaa gtttttgatg cggatacgct tttaaggttg gaaaaagtcc
    20941 tcgatttttc cgatttggaa gtgcgcgatg ccatgattac gcgcagccgt atgaacgttt
    21001 taaaagaaaa cgacagcatc gaacgcatca ccgcctacgt tatcgatacc gcccattcgc
    21061 gcttccccgt catcggtgaa gacaaagacg aagttttggg tattttgcac gccaaagacc
    21121 tgctcaaata tatgttcaac cccgagcagt tccacctcaa atcgatattg cgccctgccg
    21181 tcttcgtccc cgaaggcaaa tcgctgaccg cccttttaaa agagttccgc gaacagcgca
    21241 accatatggc gatcgtcatc gacgaatacg gcggcacgtc gggtttggta acttttgaag
    21301 acatcatcga gcaaatcgtc ggcgacatcg aagatgagtt tgacgaagac gaaagcgcgg
    21361 acaacatcca cgccgtttct tccgaacgct ggcgcatcca cgcggctacc gaaatcgaag
    21421 acatcaacac cttcttcggt acggaataca gcagcgaaga agccgacacc attggcggcc
    21481 tggtcattca agagttggga catctgcccg tgcgcggcga aaaagtcatt atcggcagcc
    21541 tgcaatttac cgtcgcacgc gccgacaacc gccgcctgca tacgctgatg gcgacccgcg
    21601 tgaagtaagc agagcctgcc cgcaccgccg tttctgcaca gtttaggatg acggtacgga
    21661 cgttttctgt ttcaatccgc cccatccgcc aaacataaaa atgccgtctg aaaccggaaa
    21721 acaggttcag acggcatttc gcttatccgc ttattttttg ctcaaagtca gccccgtcca
    21781 gccgaataac agggttaaag ccaaactcaa atagcagaaa aaggcatagg gcagatattc
    21841 ccagaccggc acacccagcg catggctgat aaatacgccg cacacgctcc acggaacaag
    21901 cgggttgatg accgtacccg cgtcttcgag cgtacgcgag aggttgcggg aatgtagtcc
    21961 gagcttatcg taaacgggtt tgaacgtttc acccgacaac aaaatactca aatattgctc
    22021 gccgatcagg aaattaaccc cgaccgaagt catggcaacg ctgaatgtgg cacgtccggc
    22081 attcgtcaaa aagctgcgga cggcatccag cagggaagga atcgcgccca gcgcaaacag
    22141 cagcccgccc aaactcatcc cgagaatcac gatggtttgc gtgaaaaaca tactttccaa
    22201 gccgccgcgc gaaatgagtt tggcgatgtc tttaaacgct tcgccttcga gtttgtagcc
    22261 gccgtaaaac cacgcgccga gctgacgcag atcgggcgtg ctgtgcagat acgttacggc
    22321 aacggcggca atgacggtaa agagcatagc gaccacggca ttgacgcgca tcaatgccaa
    22381 aacgaccaac agcgcaaacg gaatcagcga atagcagtgc accaatcccg tggcttcaag
    22441 ctggctgcgg aaggattcga cgctgttcaa atcctgcgcg gcgacgctgg gaagaagcca
    22501 cagcatcagt gccgcactga tgagccacgc gggaatggtg gtgtacatca tatttttgat
    22561 gtgttcaaac aggtcgatac cgacaatgga cgcggaaatg cccgtcgtat cggaaagcgg
    22621 ggacattttg tcgccaaaaa atgcgcccga aacaatcgcg cccgccgtca tcgccatatc
    22681 ggcctgaaac gccgccgcca tacccataaa ggcaacgccg acagtggcgc aggtggtcag
    22741 gctgctgccg atggacacgc cgatgacgga acacagcgcg aaggcggaaa aataaaaata
    22801 agtcggggaa atcagtccga aaccgtaata catcagcgtc ggaatcgcgc cgctcatcat
    22861 cagcgcgctg accatcagcc cgatgaagaa aaacaggtaa atcgcgccca taccctgatt
    22921 caacgcgcct atcatgccct gctgcatatc gttgtatttc aaaccgcgcg ccaagccgta
    22981 caaaatcagc acgacgatgg cggcaataat ggacatatgc ggcagccact ccaatgaaat
    23041 gatggtatag cccatcgcgg caatcagagc gacgacgacg gcaagtgcct caccgcgcgg
    23101 catatcgagt aaggatttga aagcgaacat gattctttgc ccgatttgat ataaagttaa
    23161 cggattctaa cacaccatac cgaatccgcc cgacgttttc agacggcatt ttagcgtttt
    23221 acaaaagccg gacagaaaca aaacggcaat tttctcaatt taaattaatt tttttgaaat
    23281 ataatttttt atttttttgt ttgttgacat ataagacttt tatggattga agtattaaaa
    23341 cctcggcaca cacgccgcaa acaccggtac gcaaaaatgc cgtctgaaag atgttcagac
    23401 gacatttctc aagaaaaaga ttatttgtcc gccctatatc cgtacaccgc aaaaaacacg
    23461 atatacagat aacagatgac ggaaacgaca aacgaagaca tcagggtgta agtatctgcc
    23521 acccagccct gcacgacagg aacgaccgca ccgccgacaa tcgcggtaca cagtacaccg
    23581 gaagccgcgt tggtaaattt tcccaatcct ttggttgcca aagagaaaat cgtcgggaac
    23641 ataatcgagt tgaaaaaacc gatggcaagc agcgaccaca tcgccacatc cgcattgccg
    23701 ctacccgtcg ccatcgcgac ggcaagcagt acgaccgcag ccgatgcgtt aaacgccaaa
    23761 taacggttgg gcgcgaattt cgccatcacc gccgaaccga ggaaacgtcc gaccatcgcg
    23821 ccgccccaat agaacgacag gtaatgcgcg gcagaagcat gatccagccc tttcagataa
    23881 cccaatacgt tgaccatcaa cgaaccgata gacacctccg cgccgacata gcagaaaatg
    23941 cctgccgtac cgaacacgag atgtttgtat tgccatacgc tggttttgcc gtcgtggttg
    24001 tgcgcgcttt cctcggcggc aattttgcgc gcgtcgggca gccggatcat tttcacgaaa
    24061 acggcaagga taatcagcag ccccgccagt cccaaatagg gaatctgtac ggaagaaatc
    24121 tgttccgcct tgctgacggt ttgggttgcg tccgccagaa tcaggaacgc gccgatttgc
    24181 ggcgcaatgg tcgtacccaa agcgttaaac gcctgaacca gcgtcagtgt tgccgattcc
    24241 ttgccgggtt tcgccagcag ggtaataagg attaccggcg acctgaagca gcgttacgcc
    24301 ggaagccaaa ataaacaacg cgcccaaaaa taccgcgtaa gaatggctac ccgcagcagg
    24361 ataaaacagc aggcatccga ccgccgtcag cagaaagccg ccgataacgc cgtttttgta
    24421 gccgactttg ccgacaaaag cccccatcgg gatggacatc accgcatagg cggtaaagaa
    24481 acagaattgg atcagcatcg cctgaacgta agacaggtcg aaaatttctt tcaaatgagg
    24541 gataaggatg tcgttcatgc aggtaataaa acccatcatg aagaacagcg tggtcaagac
    24601 gaccaatggg gacgtatgat tgttttgtga ttgcgcagac atgatttgcc tttcgtggtg
    24661 tcggaatatt acccgtttca agaaaataga aattgacatt tcttaacggc aatgcaaatc
    24721 atactgatat ttgaacattt tgcaaacttt atcggatgca ggcaatgcgc cgatctgaaa
    24781 ttcaagcagc agaaattaat attttacagt tgcttgcata gattttaata gattttaaaa
    24841 aattccggcg gacttgcaaa aaccgttccc aataaaatga aaatccgccg ccgtatccgg
    24901 aaaattcgga aatgccgcat cgtggcatca aaaccggagc ggaaagaatg tcgtatgccc
    24961 gacaaaaaag gggcatacgg ctgtgccgta ttttttgcac cgtcctccgc ccttcggcgg
    25021 gggcgtgaaa ata
//
LOCUS       DAWFJW010000018        23697 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFJW010000018 DAWFJW010000000
VERSION     DAWFJW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.018"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(107..946)
                     /locus_tag="WGR19_000616"
     CDS             complement(107..946)
                     /locus_tag="WGR19_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0798190.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1231..1306
                     /locus_tag="WGR19_000617"
     tRNA            1231..1306
                     /locus_tag="WGR19_000617"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1264..1266,aa:Arg,seq:ccg)
     gene            complement(1371..2564)
                     /locus_tag="WGR19_000618"
     CDS             complement(1371..2564)
                     /locus_tag="WGR19_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0798191.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAVTPAPSPYLPMEGLNTYRSAVQHLLFGKDNRALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKRRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYVLWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2570..3673)
                     /gene="trmA"
                     /locus_tag="WGR19_000619"
     CDS             complement(2570..3673)
                     /gene="trmA"
                     /locus_tag="WGR19_000619"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0798192.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNRW
                     YAVEFLSTLGGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGGFTQPNAAVCQKMLEWACGAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVGAAQWNIEANGIGNIKIARLSAEEFTEAYTGKREFTRLK
                     EGGIALTDYAFSTIFIDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3820..4920
                     /gene="aroC"
                     /locus_tag="WGR19_000620"
     CDS             3820..4920
                     /gene="aroC"
                     /locus_tag="WGR19_000620"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0798193.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            5012..5437
                     /locus_tag="WGR19_000621"
     CDS             5012..5437
                     /locus_tag="WGR19_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0798194.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAVAETPSVEAEAAESSTTE"
     gene            complement(5511..7496)
                     /gene="parE"
                     /locus_tag="WGR19_000622"
     CDS             complement(5511..7496)
                     /gene="parE"
                     /locus_tag="WGR19_000622"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0798195.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFFKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7563..8087)
                     /locus_tag="WGR19_000623"
     CDS             complement(7563..8087)
                     /locus_tag="WGR19_000623"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0798196.1"
                     /translation="MLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLSQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQLSGNR"
     gene            8253..9548
                     /gene="serS"
                     /locus_tag="WGR19_000624"
     CDS             8253..9548
                     /gene="serS"
                     /locus_tag="WGR19_000624"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0798197.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9621..10619)
                     /locus_tag="WGR19_000625"
     CDS             complement(9621..10619)
                     /locus_tag="WGR19_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0798198.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVDLDELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10745..11821)
                     /gene="prfA"
                     /locus_tag="WGR19_000626"
     CDS             complement(10745..11821)
                     /gene="prfA"
                     /locus_tag="WGR19_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0798199.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPIVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11925..12695)
                     /locus_tag="WGR19_000627"
     CDS             complement(11925..12695)
                     /locus_tag="WGR19_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0798200.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLETMLARYPRAWVGEIGLDFY
                     DKSQTPQQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKRTGFTWGGIVHA
                     FSGSIEEAHVLAKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12810..13799)
                     /locus_tag="WGR19_000628"
     CDS             complement(12810..13799)
                     /locus_tag="WGR19_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0798201.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHKVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13866..14549)
                     /locus_tag="WGR19_000629"
     CDS             complement(13866..14549)
                     /locus_tag="WGR19_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0798202.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14738..16072)
                     /gene="glmM"
                     /locus_tag="WGR19_000630"
     CDS             complement(14738..16072)
                     /gene="glmM"
                     /locus_tag="WGR19_000630"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0798203.1"
                     /translation="MAKKYFGTDGVRSEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTIMTNMVMEIALKEQGVDFCRAKVGDRYVLEQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16202..17059)
                     /gene="folP"
                     /locus_tag="WGR19_000631"
     CDS             complement(16202..17059)
                     /gene="folP"
                     /locus_tag="WGR19_000631"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0798204.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17199..18092)
                     /locus_tag="WGR19_000632"
     CDS             complement(17199..18092)
                     /locus_tag="WGR19_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0798205.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHDAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLNHTKGHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18183..20297
                     /locus_tag="WGR19_000633"
     CDS             18183..20297
                     /locus_tag="WGR19_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0798206.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLEAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20361..21839)
                     /gene="ubiD"
                     /locus_tag="WGR19_000634"
     CDS             complement(20361..21839)
                     /gene="ubiD"
                     /locus_tag="WGR19_000634"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0798207.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VSVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21859..22374)
                     /locus_tag="WGR19_000635"
     CDS             complement(21859..22374)
                     /locus_tag="WGR19_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0798208.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYESDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22381..22881)
                     /locus_tag="WGR19_000636"
     CDS             complement(22381..22881)
                     /locus_tag="WGR19_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798209.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYR
                     KLVLKV"
     gene            complement(23113..23682)
                     /locus_tag="WGR19_000637"
                     /pseudo
     CDS             complement(23113..23682)
                     /locus_tag="WGR19_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
BASE COUNT         5391 a         6771 c         6155 g         5380 t
ORIGIN      
        1 gcttcgtcgc cttttcctga tttttgttaa tccactataa attcccataa aaaaacggag
       61 cagatacctg ccccgttttt atttaatccg aaattttaat ctaaatttag aattttgcac
      121 cggattggtt tgccatatag tcaacagccg ctttgacttc gtcatcgctc aaaccggcat
      181 tgccgccttt ggcaggcatc gcgttaaagc cttcaagggc gtgtttgtgc aaggtttctt
      241 tgcctttttt gatacgcggt gcccaatcgt cttttttgcc tatgccggga ataccgggaa
      301 tcgaaccgcc gtggcacacc tgacaggttg cttcgaagac ttttttaccg tcaacgccga
      361 ccgcaggggc tgccgcaccc ttgtcttctg ccttcgcttc tgccggagct gcactatcgg
      421 caggagcaga agctgttcct gaagcggcat tgtcggcagg cgcagcctca tcaggattcg
      481 ggaaagaacc gccgcttttg ttcgccatgt aagtaatcgc ccgtttgagt tcctgatcgg
      541 tcaggtctgc cgcaccgcct ttggcaggca tggcgttaaa gccgttcagc gcgtgttgga
      601 acaaggtatc gaagccttgc gcgatacgcg gcgcccaatc gccgttgtgt tccagtttcg
      661 gagcgttcgg cacattgctg tccgccgcgt ggcattggat acagattttg ccgaaaatct
      721 gttcgccttg gcgttcgccg acggggatgc cgtcgcccat cgtcaattgt ccgacaggct
      781 ggatacgggt ctgcgttgct gcttccgtag tggcatcgac atcgccgaac gagccgctgc
      841 ccgccagctt aatcaggaaa taaaggactg caataacaat aacgataccg ctcacaaggg
      901 taaacagtgc agagccttgg gctttattgt cgcggagttg tttcatttgg taggcctcgc
      961 cgtcaggtta ggttgtgctg taaattatag tttggtgtgt taaacgcagt taacaatatt
     1021 ttgctggatt atactgaatt cacagggtct ttccaatcgc tatcattgaa aatatgaaaa
     1081 aatttgccaa cggtatctgt ataaaacaaa taatcctttg aaaataattg tttatcctca
     1141 agaaaactct ccttatgccg ccatacgccg cctgccggcg caagataacc tttgccaatt
     1201 tgcagaattt acgttaacct tacgttttac gcacccatag ctcagttgga agagtgtcag
     1261 tttccgaagc tggaggtcac aggttcgatc cctgttgggt gcgccaatta taaagaggcc
     1321 gtctgaaaga taaatatttt tcagacggtc ttttgactta cttcaaactc ttatttcaag
     1381 acttccgcaa atgcgcgggc aacatagtcg gtattcgacg tattcagtcc ggcgacgcac
     1441 atcctgccgg aatccagcag gtaaacggca aattcgtcgc gcagcctgcg gacttgcccc
     1501 acgctcaacc ccgtgtagcc gaacattccg cgctgtttga tgaaataagt gaaatcgcga
     1561 tcggggattt gcgcagttaa gacatcataa agtttctgcc gcatggcgcg gatgcggtcg
     1621 cgcatcatat aaacttcgtt ttgccacaag acgtaaagtt cggggctgtt catcacgtcg
     1681 gcggcgatat acgcgccgtg cgcgggcggg ctggagtaga tgcggcggac agtgaatttg
     1741 agctgtccga acaccaaatc cgcttcttcc ttattcgggc aaaccacgct taagccgccg
     1801 acgcgctcgc cgtagagcga caggtttttc gagaaggaat tgctgacgaa caagggcaat
     1861 tccatttcca ccgctttgcg gacggcgtag gcatcgctgt ccaaatcgcc gccgaatcct
     1921 tggtaggcaa tgtccataaa cggaatcagt ttgcgccttt tgatgatgtg caacacttcg
     1981 tcccattgtt gttccgacat atccacgccg gtcgggttgt ggcagcaggg atggaggatc
     2041 aggacgctgt tttcgggcag ggtgttgaaa aacgcggtca tttcgtcgaa tttcacgccg
     2101 acggcagcag ggtcgtaata tgggtaagtg ccgacctcga aacctgcgcc ttcaaaaatg
     2161 ccgcgatggt tgtcccaagt cgggtcgctg acgtaggcgc gcgcttcggg aaaccagcgg
     2221 tgcaggaaat ccgctccgac tttgagcgcg cccgagccgc ccaaagtttg cacggtaacg
     2281 atgcgccctt gcgcaagcgc gcggttgtct ttgccgaaca ataaatgctg caccgcgctg
     2341 cggtaagtgt tcaaaccttc catcggcaga tagggcgacg gcgcaggcgt gacggcgcgg
     2401 gcggtttcgg ctctgctcac ggattccaat acgggcatct tgccttcgtc gtcgaaataa
     2461 atgcctatgc tcaaattgac tttttcaggg cgcgggtcgt ttttgaaggt ttcgaccaaa
     2521 ctcaaaatcg gatcgccggg atagtattcg atgtgtcggt acatagtcct tacctcttgc
     2581 tttttccaag gattttcttt ttcaacagca caccgctttc aatatggtgc gtaaacggaa
     2641 attgatcgaa cagggcggca cgttcaaccg tatgggtttc cgccaaggtg tccaaattgg
     2701 tacgcagcgt ttcgggattg caggaaatgt agatgatgtt gtcaaactgc gacaccagct
     2761 tcaaagtttc ctcatcgata ccggcacgcg gcggatcaat gaaaatagtg gaaaatgcat
     2821 aatccgtcaa agcaatacca ccctccttaa ggcgtgtaaa ctcacgtttg ccggtatagg
     2881 cttcggtaaa ttcttcggca gacaggcggg cgattttgat gttgcctatc ccgtttgcct
     2941 caatattcca ttgcgccgca cctactgaag ttttggaaat ctcggttgcc aaaacctgtc
     3001 ggaaatatcg ggacagcggc agggtgaaat tgccgtttcc gcaatatagt tcgagtaaat
     3061 ccccacccag cccttctgcc gcaccgcatg cccattcaag cattttctga cacacggcgg
     3121 cattcggttg ggtaaaaccg ccttcaattt gccgataacg gaaatcccgg ttgccgacct
     3181 tcaaagtttc cgttacatag tcctgtttta agactatttt ctgtcccctg ctccgcccaa
     3241 taacggaaat atccaactgt tgctgtaacg cttgcgccgc ctgcatccac tcattatcaa
     3301 gccttttgtg gtaaatcatg gtaaccagca tttccccgcc gagcgtagac aaaaattcga
     3361 cggcatacca acggtttctc agttcggcag attgggcggc aacggcaatc aattctggca
     3421 tcaggcagtt gactgcctct gaagcagcat cgaagcggtc gcaacggatc aggcttgccc
     3481 caccagcctt ttgtcctttt tcaaacatgg cataaaacat ttccccgcct tcgtgccaaa
     3541 tgcggaactc ggcacgcata cggtagtgtt tgtccggaga ttcgtacact tcccactcag
     3601 gaacatccaa acctgcaaaa agggttttaa ggtggtcttt tttatcttga agctgctgcg
     3661 tgtagtcgct catatcgcta tcctgaaaaa tcagaccgga ttataagccg atttgcctcg
     3721 ccgcacgcaa actctttgac tgccgcccct tcaatgacgg acgggctttt gtgctaaaat
     3781 ccgccatctt tccacactat accgataaag ggaaaaatca tggcaggcaa cactttcgga
     3841 caactcttca ccgttaccac cttcggcgaa agccacggcg cgggtttggg ctgtatcatc
     3901 gacggctgcc cgcccggctt ggaattaagc gaagcggata tccaatttga cctcgaccga
     3961 cgcaaacccg gcaccagccg ccacgttacc caacgccgcg aagccgacca agtcgaaatc
     4021 ctctccggcg tattcgaagg caaaaccacc ggcacgccca tcgccctctt aatccgcaat
     4081 accgaccagc gcagcaaaga ctacggcaac atcgccacca gcttccgccc cggccacgcc
     4141 gactatacct attggcacaa atacggcacg cgcgactacc ggggcggcgg caggagttcc
     4201 gcccgcgaaa ccgccgcccg cgttgccgcc ggagccgttg ccaaaaaatg gttgaaagaa
     4261 aaattcggca cggaaatcac cgcctacgtt acccaagtcg gcgaaaaaga aatccggttt
     4321 gaaggctgcg aacacatttc ccaaaatcct ttttttgccg ccaaccatag ccaaattgcc
     4381 gagctggaaa actatatgga cagcgtgcgc aaatccttgg attccgtcgg cgcgaagctg
     4441 catattgaag ccgccaacgt ccccgtcggc ttgggcgaac ccgtattcga ccgcctcgat
     4501 gccgaaatcg cctacgccat gatgggcatc aacgccgtca aaggcgtgga aatcggcgca
     4561 ggttttgaca gcgtaacgca acgcggcagc gaacacggcg acgaactcac cccgcaaggc
     4621 ttcctgtcca accactcagg cggcatcctc ggcggcatca gcaccggaca agacatccac
     4681 gtcaatatcg ccatcaaacc caccagctcc atcgccaccc cgcgccgcag tatcgacatc
     4741 aacggcaacc ccatcgaact cgccacgcac ggcaggcacg acccctgcgt cggcttgcgc
     4801 gccgcgccga ttgcagaagc tatgctggca ttggttttaa tcgaccacgc cctgcgccat
     4861 cgcgcgcaaa atgcggatgt tcaggttaat acgcccgaca ttaccctttc aaacaaataa
     4921 aaatttagcc aaaacacaga ctttataata gaataccgag cattgccgtg ccgctttgac
     4981 gcacgggttt gtttagagga atacaaccga aatgacacaa gaaaccgctt tgggcgcagc
     5041 actgaaatcc gccgtccaaa ctatgagcaa aaagaaacag acagaaatga ttgccgacca
     5101 catctacggc aaatatgatg tattcaaacg atttaaaccg ttggcactcg gtatcgatca
     5161 ggatttgatt gccgctttgc cccaatacga tgccgcactg attgcacgcg tcctcgccaa
     5221 ccactgccgc cgtccgcgct atctgaaagc cttggcgcgc ggtggcaaac gtttcgattt
     5281 gaacaaccgt ttcaaaggcg aagttacccc cgaagaacag gcaatcgcgc aaaaccatcc
     5341 tttcgtgcag caggctttgc aacagcagtc cgcccaagct gtcgccgaaa cgccgtctgt
     5401 tgaagccgaa gcagccgaat cttccacaac agaataaatc cccaaacgaa atgccgtctg
     5461 aaagccgatt tggtttcaga cggcattttt tcgtatgcgg caatcacggt tcaaatatcc
     5521 aattccgccg tatcgccttc gcgttccatc caagcgcggc gggcggcggc ttcgcctttg
     5581 cccatcagtt tgaagaagat gtcgcgcgtc tcgtcatccg cgccttcggg gatttgtacc
     5641 tgcaacaggc ggcgggtgtc ggggtgcatg gtagtatctt tgagctggtc tgggttcatc
     5701 tcacccaaac ctttgaaacg gctgatggaa taagcagttt ctttaacgcc ttctttttgc
     5761 agccgctcta aaatgccgtc aagttcgttt tggtcgaggg cgtagaattt gcgggcgggt
     5821 ttgcttttac cttgtgcgtt gacatctacg cggaacagcg gcggctgggc gacgtagatg
     5881 tgtccgtcag cgaccagttt cgggaagtgg cggtagaaca gggtcagcag caaaacttga
     5941 atatgcgagc cgtccacgtc ggcatcggac aggatggcga ttttgccgta acgcaggccg
     6001 cttaaatcgg gatggtcgtt gatgccgtgc ggatcgacgc cgatggcaac tgaaatgtcg
     6061 tggatttcgg cgttgccgaa gagctggtcg gggtggactt caaagctgtt gagcactttg
     6121 ccgcgcaggg gcaggatggc ctgggtggct ttgtcgcgag cgagtttggc ggagccgccg
     6181 gcggaatcgc cttcgacaag gaagagttcg ttttcgcgga tgtcttcgct ttcgcagtcg
     6241 gtcagcttac cgggcaggac ggcgacgccg ctgccttttt tcttttcaat ctttttaacc
     6301 gaacgcatcc gcgcctgtgc ctgacggatg gcgagttcgg cgattttttt gccgaagtcc
     6361 acgttttggt tcagccacag ttccaaaggg tcgcccgata cggtagcaac gagtttcagc
     6421 gcgtcgcggt tggtcagttt gtctttggtc tgaccttgga actgcgggtc gaggacacgg
     6481 gcagagagga caaaggcagt tttgccgaac acgtcgtcgc tttgcacttt cacgccgcgc
     6541 ggtaagaggt tgtgcaggtt gatgaagttg ctgacggcgt tgaacacggc ttgtttcaag
     6601 cccgcttcgt gcgtgccgcc cagcggggtg gggatgaggt tgacatagct ttcgttggca
     6661 cacgagcctt cttccagcca agtcagggca aaggcggcgc cttcgccgat gctgaaatcg
     6721 ccgttgtgtt cgtctgaaat gtagttttcg caagagaaca gcggtacggc ttcctgcgcg
     6781 tcggcaatca ggtcggtcag atagcttttc aggccgtcgg ggtaatgcca ggtttgggtg
     6841 tgcgcttcgt cttcgccttt gaccggacgg gtcagggaaa cgcgcacacc cggcagcagc
     6901 acggctttgg cacgcagcag gcgttcgagt tcgggaatgc tgtaattcgg gctttcaaaa
     6961 tatttgccgt ccggccagac gcgcacttcg gtgccgctgt ctttgacggc gcatttgccc
     7021 acttgtgcca acggttcgac cacgtcgccg ccggcaaaca cgatacggtg gattttgcct
     7081 tcacgtttga ccgttacctc aaggcgggtg gagagggcgt tggtgacgga tacgcccacg
     7141 ccgtgcagtc cgcctgaaaa ggcatacgcg ctgccgccgt cttttttgtt gaacttgccg
     7201 cccgcgtgca gacgggtgaa cacgagttcg actacgggta cgccttcttc agggtgcagg
     7261 ccgacgggaa taccgcggcc gttatcacgg acggaaagcg aaccgtcttc atgaatttgc
     7321 acgtcgattg cagtcgcgaa accacccaac gcctcgtccg ccgcattatc aatcacttcc
     7381 tgacagatgt gggtcgggct gtcggtgcgg gtgtacatgc cgggacgttc tttgaccggc
     7441 tccaagcctt tgaggacggt gatgctggat tcgctgtatt ggttgttttt agccatagga
     7501 ataatctgaa agtaagaaaa acaacgcttt cagacggcct gaaagcgttg cgttccgttg
     7561 ttttaccggt tgccggaaag ttggcgggcg gcaaagtctt cataactttc cataccgcgc
     7621 aggaagcggg aagaaagttc tttcaatgcc cccaaataaa cgtcgcgttt gaaatcaatc
     7681 acttcgtcaa cgggcgccca atattggtgc caacgccagc cgtcaaattc ggggtggcgg
     7741 gtggcgcgca ggttgacatc gcaatcccgg ccggtcaggc gcaggagata ccaaatctgc
     7801 ttctgcccgc gataagagcc gcgccattcg cggcgtaccc agttgttcgg tacgtcgtaa
     7861 cgcagccagt cgcgcgtccg cccgacgatt ttgacgtgtt gcgacagaag tccgacttct
     7921 tcgtaaagct cgcggtacat cgcggtttcg gggctttcgc ccggcttgat gccgccttga
     7981 ggaaactgcc atgaatgttc gcgtacgcgc ttgccccaaa agacctcgtt gcggttgttg
     8041 attaagatga taccgacatt ggggcgatag ccttccctgt ccaacacggt gtcgccctcc
     8101 gttaaattca atcttgggat tttcccacaa atcggacggt tttgacaaat cagacggtat
     8161 ggttcaagca tgtcgaaaca cggacggatt cgggaaaata tcttaaattt ggtttacaat
     8221 aatgtgtttc aaattaattc gggaatcaga ccatgttaga tatccaattg ctccgcagca
     8281 acaccgccgc cgttgccgaa cggcttgcac ggcgcggtta tgactttgat accgcacgtt
     8341 ttgacgcact ggaagaacga cgcaagtccg ttcaggtgaa aactgaagaa ctacaagcct
     8401 cgcgcaacag catttccaaa caaatcggcg cactgaaagg tcagggcaaa catgaagagg
     8461 cgcaggcggc catggatcaa gtcgcccaaa tcaaaaccga tttggaacag gcagccgccg
     8521 atttggatgc cgtgcaaaag gaactggacg catggctgtt gagcattccg aacctgccgc
     8581 atgaggacgt gcctttcggt aaggatgaaa ccgaaaacgt cgaagtccgc aaagtcggca
     8641 ccccgcgcga atttgacttt gaaatcaaag accatgtcga tttgggcgaa cctttgggtt
     8701 tggattttga aggcggtgca aaactctccg gcgcacgatt taccgtgatg cgcggacaaa
     8761 tcgcccgtct gcaccgcgcc ttggcacagt tcatgctgga tacgcacacg ctgcaacacg
     8821 gctacaccga gcattacacg ccttatatcg ttgacgatac gacgctgcaa ggtacgggcc
     8881 aactaccaaa atttgcggaa gatctgttcc acgttacccg tggcggcgac gaaaccaaaa
     8941 ccacccaata cctgattccg acagccgaag ttaccctgac caataccgtt gccgacagca
     9001 ttatcccgtc cgaacaactg ccgctgaagc tgaccgcgca ttcgccctgt ttccgcagcg
     9061 aggcgggttc gtacggcaaa gacacgcgcg gtctgattcg ccagcaccag ttcgacaaag
     9121 tggaaatggt tcaaatcgtt catcccgaaa aatcatacga aacgctggaa gaaatggtcg
     9181 gccatgccga aaacatcctg aaggctttgg aactgcccta ccgcgtgatt accctgtgta
     9241 ccggcgacat gggcttcggc gcggcaaaaa cgtatgactt ggaagtttgg gtgccggcgc
     9301 aaaataccta ccgcgaaatc tcaagctgct ccaactgcga agatttccaa gcccgccgcc
     9361 tgaaggcgcg tttcaaagac gaaaacggca aaaaccgctt ggtacatact ttgaacggct
     9421 ccggtttggc agtcggcaga accttggtcg ccgtgttgga aaaccatcaa aacgccgacg
     9481 gcagcatcaa catccctgcc gcactgcaac cgtatatggg cggcgttgcc aagttggaag
     9541 tgaaataagc cttaaccctg aacataaatg ccgtctgaaa cctggttcag acggcatttc
     9601 ctttaaactt ttaaaacaca tcaaccgtcg gcacgaaccg cattgccgca atcgccggtc
     9661 tgtccgacct cgcggatatt ggacagcgta acttccgaaa tattacccaa cgcctcttcc
     9721 gtcaaaaacg cctgatgtcc ggtaaacagc acgttatgac aagacgacag gcggcggaac
     9781 acgtcgtcgg taatcacatc gttggatttg tcttcaaaaa acagctcgcg ctcattctcg
     9841 tacacatcca tacccaatgc gccgattttc cggcgtttca acgcctcaat cgcggcggca
     9901 ctgtcaatca gcccgccccg gctggtgttg ataatcatca cgccgtcttt cattttgtcg
     9961 aacgccgctt cgttcagcat atagtggttt tccggcgtgg aggggcaatg cagcgtgatg
    10021 atgtccgacc gggcatacag ctcgtccaaa tccacatacc tgccgccgat tttttccgct
    10081 tcagggttgc aaaacggatc gtaagccagc aggttcatgc cgaaaccctt taaaatccgc
    10141 atggttgcaa taccgatttt ccccgtgccg ataacgcccg ccgttttgcc gtacatattg
    10201 aaaccggtca gaccttccag cgaaaaattc gcatcgcggg tacgctgata ggctttgtgg
    10261 atacggcggt tcaaagtcag catcagaccg accgtatgtt ccgcaaccga ttcgggcgaa
    10321 taggctggca cgcgcacgac tttcaagccc aactcttcag ccgctttcaa atccacatta
    10381 ttgaagccgg cacaacgcaa cgccacagtt ttcacgccaa tttgcgccaa tttttccaac
    10441 acgggccggc tgccgtcgtc gtttacaaaa atacaaaccg cttccgcgcc ttccgccatt
    10501 ttcgccgttt tcgcatccag cataaaatca aaaaactcca gctcgaagcc gaaatgccgg
    10561 ttggcgcggg taaaatgttc gcggtcatag cttttcgtac cgtaaatcgc aatcttcatc
    10621 aagtatgtcc agttgtcgtc tatggttgag aaacggcata atacactgaa ttcaaacaaa
    10681 tcagtagaat atggtggatt aaaattgatt gcatgcacgg catttccatt tcaaaacaca
    10741 aaactcaatc gcccattgcc gccaaaagct ccgcctgatg ttcggcaatc agggcatcgg
    10801 tgatttctgc caaatcgccg tccatcacaa aatccagctt gtgcagggtg aggttgatgc
    10861 ggtggtctgt taccctgcct tggggatagt tgtaggtgcg gatgcgttcg ctgcggtcgc
    10921 cgctgccgat gagggatttg cgttcggcgg cttctttggc ttgggcttcg cgtttttgcg
    10981 cgtcgttcag gcgggcggcg agaactttca ttgcctgcgc tttgttggcg tgttggctgc
    11041 ggccgtcttg gcattcgacc accatgccgg tgggcaggtg ggtgatgcgg acggcggaat
    11101 cggttttgtt gatgtgctga ccgcctgcgc cggatgcgcg gaaggtgtcg atgcgaaggt
    11161 cgacggggtt caactcgatg tcttccagtt cgtccgcttc gggcatgacg gcaacggtgc
    11221 aggcggaggt gtggatgcgg ccttggcttt cggtggcggg gacgcgctgc acgcggtgtc
    11281 cgcccgattc aaatttcaaa cggctgtacg cgcctaagcc gataatacgg gcgataactt
    11341 ctttatagcc gcccaactcg ctttcgttgg cggacacgat ttcaacctgc cagcggttgc
    11401 gttcggcgta gcggctgtac atacgcagca aatcgccagc aaacagcgcg gcttcgtcgc
    11461 ctcccgttcc ggcgcgtact tcgataaaga tgtttttgtc gtcgtcggca tctttgggca
    11521 gcagcagttt ttgcaattcg gtatcgagtg cgccgatttt ggctttcgcc gcttcgattt
    11581 cttcggcggc aaagtctttc atttcggggt cggacaacat ttcttcggca tctgccaagt
    11641 cgctttgggc aagccgatag ttttggaata cttcgacgat gggggtcagt tcggcgtgtt
    11701 cgcgcgtgag cttgcggtag ttgtccatgt cggacgtggc ttcgggttgc cctaaaaggt
    11761 gggtaacttc ttccaaccgg tcgccgagtt gttgcagttt ttctaaaata gacggcttca
    11821 taattcttcc ataacaaacg ccgcctgaat ttcagacggc atcaacactg aattattata
    11881 ataggttttc cggacattca aaaagataat ctcagatgga taacctaccg tcccaacagg
    11941 gcatcggcat tgcgctccgt taccttggca atctcttcca cacaggttcc gcggatttcc
    12001 gcagcaatct ttgcaatacc cggaatattg gcaggcgtat taatctcttt tttcagcata
    12061 aacgggctat ccgtttccaa tacaaaatcc ccgtcgttca aggctttaag cgtatcgcgc
    12121 actttacgcg cgttcggatt gagcagcagc gaaccgatgc cgattttgaa acccagtttc
    12181 gccaacacgt gcgcttcttc aatactgccg gaaaaggcgt gaacgatgcc gccccaggta
    12241 aagcccgtcc gtttgacggc ggcggcgatg tcggcggtgg ctttcagatt atggataatc
    12301 acgcggcggc gcagggtttg cgcgatggca agctggcgga cgaaaacttg aatttgccgt
    12361 tcgcgctgct gcggcgtttg gcttttatcg taaaaatcca agccgatttc gccgacccat
    12421 gctcgggggt aacgtgccaa catcgtttcc aggcggacgc aatcacgctc ggcaatgccg
    12481 tctgaaaacc aaggatggat gccgagcgca atacggattt gaccgtgttc ggacggcatt
    12541 tccgacagtt ccgccacgct ttgccaatcc tgcggacagg tggcgggaac gataaaccgc
    12601 ttcaccccaa ctttccgcgc ggcggtcagg atgtgcggca ggttttcgcg cagggcggca
    12661 tcggcaaggt ggcagtgggt gtcggtgaag ttcatttcga ttccgcaatg cggcaggttc
    12721 acataaaaat aaggcagggc ggcaagggat gccgtgtacg ggcggcgcat aaggcgcggt
    12781 caacgccccg tcattgccat ttcaatccgc tataacttca gtcttgccaa ctctttttcc
    12841 acctcttcct taccccagcc ttgcgatacg gcgagggtca taagcgcggt ggcggtttcg
    12901 aggttgcatt tgccgccatt gatgatgcct gagttgcgga acgcgttgcc ttgcgcgtaa
    12961 acggcagcgg ttctgccttg tcggacttgg ctgatgttga gcagcagttt gccttgctgc
    13021 gtaaagtctt gcacggcgcg gatgaaacct tcgtctgcgg gcgtgttgcc gtgtccgtag
    13081 ctttgcagga taagggcttg ggcgggaagc tgtccgagtc cgtccgcaag ttcttggacg
    13141 gcaaagccgg ggataagcgt gcggacagcg atttttgcct gcgggtcggg ataacggatt
    13201 ttgaggccgt ctgaaacatc tgccgcgtcc tgattcggga tgcggacttt gtgccagccc
    13261 tgggtttcgt cccactccgc cagtgcgccg aaatgcggat tgtcgaagcc tgcggcggtt
    13321 tcagtgctga ctttgctgct accgacggcg ggatacagtt tgccgtcaaa cgcgatgacg
    13381 gtttgtttga gcttgaggct gaaggcggca acggcagtcg ccagattgcg cggcgcgtcg
    13441 ctgcccacgg cgttgtacgg ccattgggaa ccggtcagga cgatcggctt gtccaaaccc
    13501 tgcagcgcga gggcgaacag attggcggta taggcgagcg tgtccgtacc gtgcagcacc
    13561 aaaacgccgt cgtactgcgg cagcgcggcg gcaatgatgt ccagccaagc gcaccagttt
    13621 tgcagttgga cggcggaaga atcaatcagc gtcccacaaa cgtgccaatc gaaatccatg
    13681 ccgtttgaaa acggcgcgag tgcctgcccg accagcgcgg tatcggggcg caggccttcg
    13741 ctgctttgcg tcatgccgat ggtgccgccg gtataaagga caaaaatttt ctgtttcatg
    13801 gctcatcctg aaaatgcaaa gaccgcctga aacgcgaaat gcgtccggac ggtcgaaaat
    13861 ctgatttact gctgcttctg tgccgctttt ttcgctgcct tttccgcctt gcgtttggcg
    13921 cgtttttcgc tcaattgtgc gcggtaaagc tgataatgtc ggcgtttgcg ccaccagaac
    13981 cacgccagcg ccgccgccag cacgcccaat gcgatgaaga tgccggattg caggctgtgc
    14041 attttcgcca tcagccaatc gatgttgtgc gcgccgtact cgcccaagta aatccaaacg
    14101 ggcacggaaa tcagcgcggc aagcccgtcc ataatcagaa agcgcagata cgatactttg
    14161 cggctgatgc cggcggtaac gaaaacggca gtccgcaaac cgggcaggaa acgagcgaca
    14221 aataacaccc agttgccgta tttgtcgaat ttttcctgaa cctgtgcgta acgtttcggc
    14281 gtcatgatgc gcgcaatcgg tttgaacttg aggattttct gcccccagat gcgtccggcg
    14341 gcgaacatga tgccgtcccc gaccaatacg ccgagcatac cgactgcaaa cataatatgc
    14401 ggattggtat aacccatacc cgaaatcacg ccgcctgtta ccaaggtcaa atcctcggga
    14461 atcggcacgc cgaaaccgca gatgaccaaa acgaaaaaca cggccgcgta gccgtattcg
    14521 acaaaaaagg cttccaaaag ggcaaacata gcggatattc cattgtcgga gataaaaagt
    14581 cagaacaagc cgaaacattt tctgcgcgaa acggcattct atcaaagatt atgccgtctg
    14641 aaagcggcaa ggggcagatt cgcgccctct gccggacgtt cagacggcat ttaaacagga
    14701 aaaccgccgc tgttgaaacg gttttgcctg attttgccta aaggctgccg atggcggaag
    14761 caatacgttc cgcaccgtcg cgcgcccaat ccgcctgccg cgcctccacc atcacacgca
    14821 cgacgggttc ggttcccgaa gcgcgcaaca cgacacgccc tttgccttcg agttcttttt
    14881 ccacttccgc caacacgtct tttgaggctt cctgccagtt ttgccctttg ccgatacgga
    14941 cattaatcat cgtttgcgga tacggctgcc aatcggcgca aacggtggcg aggtcttggt
    15001 tcagcgtttg cagtgccgcc aaaacttgca gtgcggaaat aatgccgtcg ccggtgttgt
    15061 gtttgtccat acacaaaata tggccgctgg cttcgccgcc gatgagccag ctgcgttggt
    15121 tcagctgttc caacacatag cggtcgccga ctttggcgcg gcagaaatcg acgccctgct
    15181 ctttcagggc gatttccatc accatattgg tcatgatcgt gccgaccacg ccgccgatgt
    15241 tgataccttc tcgggcgcgg gctttggcga tgacgtaaat caggctgtcg ccgtcgtaaa
    15301 ccttaccgtt tttatcgacc atcatcaggc ggtcgccgtc gccgtctaag gcgatgccgt
    15361 agtcggcttc atgctgtaaa acggcggcct ggagtgtctt ggtataagtc gcaccgcatt
    15421 tctcattgat attgtagccg ttgggttcgt cgccgatgct gacaacctgt gcgcccagtt
    15481 cgtgaaacac cttgggtgca acaccgtacc ccgcgccgtt ggcggtatcg acaaccaact
    15541 tcaaaccccg aaggtcggaa tggctgggga aggtggattt gcaaaattcg atatagcggt
    15601 cgtccgcacc gctgatgcgg cgggcgcgtc cgaggcgtgc agaaggctgg gttttcattt
    15661 cttcgtcgat tttggcttcg atttccaact cgatttcatc gctcagtttc acgccgccct
    15721 cggcaaagaa tttgatgccg ttgtcggaat aggcattatg cgacgcggaa atcatcacac
    15781 cggcggacaa acgcagcgca cgggtcagat aagccacgcc gggcgtaggc agcggaccgg
    15841 tttggataac gttgacacct gcggcggtaa aacctgccac caaagcagct tccagcatat
    15901 aaccggaaat acgcgtatcc ttgccgatta aaacggtcgg tttctggtcg gtgtcgtgct
    15961 gaaccaaaac ctgacccgcc gcatagccga gtttcaatac aaaatcgggc gtaatcggaa
    16021 actgcccgac ttcgctgcgc acgccgtccg tgccgaaata tttttttgcc atgtgttgct
    16081 ccgagaatgt gaaccgttgt ccgagattat acagtcagtt tgtgccttgc tgtctgcacc
    16141 gttgatgccg tctgaaaccg ccccgtcctt ttcagacggc atgaagtatg tgaaccgctg
    16201 tttacagatt gatgcccaac gcttcccaca ccttcaacgc atccgctgtc gccttcacat
    16261 catgcacccg cacgatttgc gcgccgcgcg ctacggaagc caacgctgcc gccacgctgc
    16321 cgtgtacgcg ttccgccgca tttgcctcgc cggtcagctc gcctatcgtg cttttgcgcg
    16381 atacgccgat gagcagcgga aaacctgttt ccgccatcaa ttcgggcaaa tgccgcatca
    16441 gcgcgatatt gtgttgcaag ggtttgccga agccggagcc gaagccgggg tcgagtatga
    16501 tgcgttgcgg tgcgatgcct gccgcgatac attccgctga gcgcgctttc aaataccgcg
    16561 ctacttcacc gacaacatct tgatatttcg gattaatctg catggttttg ggcaaaccct
    16621 gcatgtgcat caggcaaatg cccgtgtccg cctgacgcgc cagcaattcg accgcgccct
    16681 cgtcattcaa cgccgccaca tcattaataa tatcgacgcc gccgagtgcc aacgcttttt
    16741 ccataatcac cgtgcggcgc gtgtccaaac tgatgggaac gccccacccc gccacttccg
    16801 ccaaaacagg ctcaacccgc gcccattctt cttcaggcga aacataatcc gcacccgacc
    16861 gcgtcgattc gccgccgatg tcgagaatgt ccgcaccctc tttcaaaagc cgttcggcgt
    16921 gcgccaaagc tgtttgggca ttttgcgaat acgcgccgcc gtcggaaaaa gaatcgggcg
    16981 tgagattcac gatacccatg attttcggtt tgtccaaacc gatttcaaac cgtcctgcct
    17041 gccaaacgtg tcgtgccatc tgaactcctc ccaaaataaa aaacagatta tatgccgtct
    17101 gaaaccgtct tgtgcgcttc agacggcacc gctattcagg cggcagacgg catgttgtcc
    17161 gaatgtctgc tccgcctttg aatctgccgg tatgcctgct atccgcccga cttttcaaaa
    17221 caggttccga cgattccgca cgcgcctgcc gcctttgcca agccgtacag gatttcctgc
    17281 ggcatatcgc ggttccataa tcccgtaata ttcgcaatca cgggcagatg gctgatttgg
    17341 cggactttca cgatggattc gacatccaaa cggtagggat ggcctttggt atggttcaat
    17401 acgcccgact cgcccagaat caggtggcgg ttgccgcgcg agacgacata ttctgcggca
    17461 ttcaaccaat cttcggcaga atggtgcttg tctttacaca gcaccaaggg aatgttcagg
    17521 tttccggctt cattcaatac ggcaagatcg gacatcagcc cgccgcccaa atacaggatg
    17581 tccgcccccg cattcaaagc cgcttcgaca tggcggacgt tgcggacgcg caccaatacg
    17641 ggtttccctg catcatgcgc cgatgcggtc tgttccgcca accgtctgca ccgtccccgc
    17701 ccttcatccg cacttgaagt gtcgtataag tttgccgaag tgaaaaacgg atccagaaac
    17761 actgcatccg cattgcgcca tactgacggt tctgcggcga tacagacggt ttccccgccg
    17821 ccgaaagcca cgcctttggc ggcaacgcgg ctgtcttccg cccgattttc ccgactgacg
    17881 gttttccatg tatccaaaat gcggacggct ttctcgacct ccggcagcgt ctgcacctcc
    17941 ctgacgctca aaaccctatc gtcgccgatt gcgccgatga cggtacgctc gtcgccgtga
    18001 gaaatgtgtt ctcgcagacc tctgctgcgg ataaaggcga caacgccggc aatgtccgct
    18061 tcggcggcac gcctgctcat gacaataatc atatttcctc ctgacacaag aaacggcctg
    18121 cccaaaatag gatttttgca agccgtgtta tactgtggcg tgttttacag attgttcggg
    18181 ctatggattt attatcggtt ttccacaaat accgtctgaa atatgcggtg gccgtgctga
    18241 cgatactgct tttggcggca gtcgggctgc acgcttccgt atatcgcacc ttcacgcctg
    18301 aaaacatccg cagccgcctc caacaaagca ttgcacacac acaccggaaa atctcgtttg
    18361 atgcggacat tcagcgcagg ctcctgcccc ggccgaccgt catcctgaaa aacctgacca
    18421 ttaccgaacc cggcggcgac cagactgccg tttccgtcca agaaaccaaa atcggattga
    18481 gctggaaaaa cctgtggtcg gatcagatac agattgaaaa atgggtggtt tcgagtgcgg
    18541 aacttgccct gacgcgcgac gggaaaggtg tttggaacat ccaagacctg atcgacagcc
    18601 aaaaacgcca agcctcagtc aaccgcatta tcgtcgaaaa cagcaccgtc cgcctcaatt
    18661 tcctgcagga acagcttatc ctgaaggaaa tcaacctcaa cctgcaatcc cccgattcgt
    18721 cggggcagcc gtttgaaagt tcgggcatac tggtttgggg aaagctgtcc gtcccgtgga
    18781 aaagcagggg gctgttcctt tcagacggca tcggcacgcc caaaatctca ccgttccatt
    18841 ttgaagcttc cacttcgctg gacggacacg gcattaccat ttccaccacc ggcagccctt
    18901 ctgtccgctt caacgccggc ggagcggatg ccgccggcct cggcctgcgt gcagacactt
    18961 ccttccgcaa cctccacctg accgcccaaa tccctacgct ggcactcagg aacaacagca
    19021 ttaaaattga aaccgtcaac ggcgcattta ccgccggcgg cgaatatgcc cgatgggacg
    19081 gttcgttcaa actcgacaaa gccaacctgc actccggcat cgccaacatc ggcaacgccg
    19141 aaatctccgg cagcttcaaa acaccgcgcc accagaccaa cttctccctc aattcgccgc
    19201 tcgtatggac ggaaaacaaa gggctggagg cgccgcgcct gtatgtatcg acccttcagg
    19261 ataccgtcaa ccgcctgccg caaccccgtt tcatcagccg gctcgacggt tcgctgtccg
    19321 taccgaatct gcaaaattgg aatgccgaat taaacggcac attcgaccgc caaaccgttg
    19381 ccgcgaaatt cagatacaca catgaagacg caccgcatct ggaagccgcc gtcgcactgc
    19441 aaaaattgaa cctgaccccc tatcttgacg acgtgcggca acaaaacggc aaaatatttc
    19501 ccgacaccct cgccaagctg tccggcgaca tcgaggcgca cctgaaaatc ggaaaagtcc
    19561 aacttcccgg cctgcaactg gacgatatgg aaacctacct ccacgccgac aaagaccata
    19621 tcgcgctcag ccgtttcaag tcagggcttt acggcggtca taccgaaggc ggcatcagca
    19681 tcgccaacac ccgtcccgcc acttaccgcc tgcaacagaa tgcaagcaac atccaaatcc
    19741 aaccgctgct gcaagacctg ttcggcttcc acagcttcag cggcaacggc gatgcggtca
    19801 tcgacctgac cgcgggcggc gaaacccgaa aagagcttat ccgctcgctt cagggcagcc
    19861 tgtcgctgaa tatttccaac ggcgcgtggc acggtatcga catggacaat atcctgaaaa
    19921 acggcatttc gggcaaaact gccgacaatg ccgcacccag cacacccttc caccgattca
    19981 cgctcaacag cgaaatttca gacggcatca gccgccacat cgataccgaa ctcttctccg
    20041 acagcctcta tgttaccagc aacggctata ccaatctgga tacgcaggaa ttgtctgaag
    20101 atgtccttat ccgcaacgcc gtccatccga aaaacaaacc gattcccctg aaaatcaccg
    20161 gtacggtgga caaaccgtcc attaccgtcg attacggcag gctgaccggc ggcatcaatt
    20221 cgcgcaaaga gaaacagaaa atcctcgaag acaccctgct ggaacaatgg cagtggctca
    20281 aacctaaaga accgtaaaca tcctgcgtac aaaaatgccg tctgaaacac ccccgcgctt
    20341 cagacggcag accgtaaaac ctacaacccc aattcctccc aaatcccatc aatcttagcc
    20401 gtaaccgcag ggtctttttt gatgacgcgt ccccattcgc ggtcggtttc gcccggccac
    20461 ttgttggtcg cgtccaaacc cattttgccg ccgagtccgc tgacggggct ggcgaagtca
    20521 agataatcga tgggcgtgtt ttctaccaaa acagtgtcgc gtacggggtc catgcgcgtg
    20581 gttactgccc agatgacttc tttccagtcg cgcacgttca catcgtcatc caccacgata
    20641 ataaatttgg tatacataaa ctggcgcagg aacgaccagc agcccatcat cacgcgcttg
    20701 gcgtgtccgg cgtactgttt tttcatgctg accaccgcca tgcggtagga gcagccttcg
    20761 ggcggcaggt aaaaatcggt gatttcgggg aactgctttt gcaaaagcgg tacgaacact
    20821 tcgttcaacg ccacgcccaa aacagcgggt tcatcgggcg gtttgcctgt gtaggtcgaa
    20881 tggtaaatcg ggttttcgcg catggtgatg cgctcgaccg taaatacagg gaaatgatcc
    20941 tgctcgttgt aatagcccgt gtggtcgccg tatggacctt ccaacgcggt ttcgttcgga
    21001 tgaatcacgc cttccaacac gatttctgcg cgtgcaggca cttgcaaatc gttgccgata
    21061 catttcacca gctccgtccg cgaaccgcgc agcagtccgg caaactggta ttcgctcaag
    21121 gtatcgggaa caggcgttac cgcgcccaaa atggtggcgg ggtcgcagcc gagcacaacg
    21181 gagacgggat acggcgtatc aggattgagt ttgcggaatt cctgataatc caacgcgccg
    21241 ccgcgatgcg acagccaacg cataatcagc ttgtttttgc cgatgagttg ttggcggtaa
    21301 atgccgagat tttggcgttt tttgtgcggc ccgcgcgtga cggtcaagcc ccatgttacc
    21361 agcggcgcaa cgtcttccgg ccagcaatgc tgaatcggaa gttgatacaa atcaacgtct
    21421 tcgccttccc acacgatttc ctgacacggc gcgtttttca ccacgttcgg cgccatgctc
    21481 caaatgtctt tcaacagcgg cagtttggaa aacgcgtctt taatgccttt gggcggttcg
    21541 ggttctttca aatacgccag cgtctgcccg atttcgcgca gcttggacac gctgtccgcg
    21601 cccatgccca tcgccacacg ttcgggcgtg ccgaacaggt ttgccaacac aggataaccg
    21661 tagcgcgtac cgtcgggctt aaccgggttt tcaaacagca acgccggccc ttcggcgcgc
    21721 agcacacggt cggcgatttc ggtcatttcc aaatgcgggg aaacggggtg cgcgatgcgt
    21781 ttgagtttgc cctgctgctc gagcatggcg atgaagtcgc gcaggtcttt gtatttcata
    21841 ttcatccttt ttgtcctttt atcctgagca atccgattcg gataccgccc ctatccttgc
    21901 ctgcgcttcg gcatattcta tgccgtgata aaagtcgcgt accagcggat gttcgctgcc
    21961 ttgatggagt tgcaacaaag gacgttgacc atcgggttgg gtaacgacat tgcaatgcaa
    22021 accgaaggtg tcggattcgt aagggggcag ccggttgcag atcatcccga aataaacggc
    22081 gttttcaggg ttgtcgtaaa agcggctttg atagtcgtta aaactctttt cgctgacgga
    22141 cacccacacg ccgtattcca gcgtttcctc atgcccgata atcggaatag gcagcactgc
    22201 gcggataaag cggtcggttt ggtcgggata gcggatgatg cagaaatcag aatcgcattc
    22261 tgcttgataa gcaatgcgtt cttcttcact gagttgatta tagggatcgg gggcggtaaa
    22321 gccgattgcg ggcatatctt catgtttttc gccgcaggaa gtgcaagtgt acataaggtt
    22381 tcagactttc aaaacgagtt tgcggtaaag ccattcgccg gcaaacggca tccccatcaa
    22441 tacataggca atcacgccgg tataaaccgc ccaccaatca tatcgcccca accgtgccaa
    22501 caaagcggca agcgtcccgt tggcaataaa gaatacgcac caaacctgcg ttacccggcg
    22561 ggtatagcgc acagcttttt caggcaggtc gggctgttgc agccgcgcaa gtttttcaat
    22621 caccgtctgc ccggcaaaca ggctgccgcc gaacaccgcc aacatcatca gattgacgag
    22681 gacgggatac caatacatcg aatcatgccg cccgaacacc aatactgcgg caaaaaacag
    22741 tgtaataaac aaagccgcat aacgctgttg gggcagtttg gcagtcaggg cgcgcagcag
    22801 ccacaacccg cacatcgccg ccgccagcca gacaaaccag cccgcctccc tgccgtaata
    22861 tagtggatta acaaaaacca gtacagcgtt gcctcgcctt gccgtactgt ctgcggcttc
    22921 gtcgccttgt cctgattttt gttaatccac tataccacaa agcgggatag gcaatgctta
    22981 atacggtcag aaaaatatgt ccgaaaaaac cgggtttcat tttgaatccg cacaaatgtt
    23041 ttcagacggc atccgataaa aacatgccgt ctgaaaaata attagaaata cccgattagc
    23101 ccgcctgaat cttcaatacc gcctgtacca cgtcgttgac ggtgcggaca ttgcggaaat
    23161 cttcagcctg cagcttgcgg gctgccacag ccattcacga ttgcgatata cccggcggca
    23221 ttcccgactg ccgttcagca gaaagcgcac ccattccaaa ccgctccaat atgcctcggg
    23281 cagcataccg gcttcagacg gcatatcgtc cgaagcagat aaagtcaggc tgtaacgcgt
    23341 ccctttggtc agaaccatcg ccaaagcata agcaaacggc gcgcgcgttg ccgatacggc
    23401 atatccttcc ggcagcggat cgtccgccgc caaaaccaaa accgacccgc atccctcttc
    23461 caacagtgat gccgcttccg ccaatgccgt ttccacaccg tcggcacaca cggccaatgc
    23521 cgtctgctcg ctcatatccc gcctcaatat cgaccattac cccgccgttg cattgtgcac
    23581 cgacaaaccg aacgaagtcg gcgacacggt ttgcgatttc aacagttcca accacaaatc
    23641 gaaactgcgc gccatttccc cgtcgtgcga ggcataaact atagtggatt aaatttt
//
LOCUS       DAWFJW010000019        23447 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFJW010000019 DAWFJW010000000
VERSION     DAWFJW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.019"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(135..470)
                     /locus_tag="WGR19_000638"
     CDS             complement(135..470)
                     /locus_tag="WGR19_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0798210.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            599..1504
                     /gene="mtrA"
                     /locus_tag="WGR19_000639"
     CDS             599..1504
                     /gene="mtrA"
                     /locus_tag="WGR19_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0798211.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDIRQNGAFTVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1566..2054)
                     /locus_tag="WGR19_000640"
     CDS             complement(1566..2054)
                     /locus_tag="WGR19_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0798212.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2201..3103
                     /locus_tag="WGR19_000641"
     CDS             2201..3103
                     /locus_tag="WGR19_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0798213.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLAEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3144..4274)
                     /locus_tag="WGR19_000642"
     CDS             complement(3144..4274)
                     /locus_tag="WGR19_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0798214.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4479..7103
                     /gene="alaS"
                     /locus_tag="WGR19_000643"
     CDS             4479..7103
                     /gene="alaS"
                     /locus_tag="WGR19_000643"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0798215.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSEQA
                     IVLLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLASVESWLCQKLS"
     gene            7165..7896
                     /locus_tag="WGR19_000644"
     CDS             7165..7896
                     /locus_tag="WGR19_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798216.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8205..11017
                     /locus_tag="WGR19_000645"
                     /pseudo
     CDS             8205..11017
                     /locus_tag="WGR19_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            complement(11735..12418)
                     /locus_tag="WGR19_000646"
     CDS             complement(11735..12418)
                     /locus_tag="WGR19_000646"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0798217.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            12715..15018
                     /gene="parC"
                     /locus_tag="WGR19_000647"
     CDS             12715..15018
                     /gene="parC"
                     /locus_tag="WGR19_000647"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0798218.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLESNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSG
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15038..16613
                     /locus_tag="WGR19_000648"
                     /pseudo
     CDS             15038..16613
                     /locus_tag="WGR19_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            16606..17073
                     /locus_tag="WGR19_000649"
     CDS             16606..17073
                     /locus_tag="WGR19_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ0798219.1"
                     /translation="MTELQHPVLVVDNETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            17177..17929
                     /locus_tag="WGR19_000650"
     CDS             17177..17929
                     /locus_tag="WGR19_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0798220.1"
                     /translation="MTDILIDNTATETVRTLIQAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(18500..19462)
                     /locus_tag="WGR19_000651"
     CDS             complement(18500..19462)
                     /locus_tag="WGR19_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0798221.1"
                     /translation="MRNAVGLDISKLTFDAFAMVGNAEYSAKFDNNSKGLNQFSDWLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTDKQDAK
                     LIAEYCRSAQESELVKRQKPTDEQYRLSRMTAAYTQIKSECAAMKNRHHVAKDEEAAK
                     AYAEIIKAMNEQLEVLKAKIKEQTEKPKCKEGVKCLETIPGIGRMTAAVLFQYLTSSA
                     FETSNKFAAFAGLSPQQKESGTSVRGKGKLTKFGNRKLRAVLFMPAMVAYRIRAFPDF
                     IKRLEAKKKPKKVIIAALMRKLAVIAYHVHKKGEDYDPSRYKLA"
     gene            complement(20315..21403)
                     /locus_tag="WGR19_000652"
     CDS             complement(20315..21403)
                     /locus_tag="WGR19_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HEZ0798222.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIVGFVGWKSFGMF
                     QIYSKATGGQIEQEAQKESVVQQTMTEQTASSETATFEHSKNLKPEDFTPTLPEKPES
                     KPIYNDVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPY
                     KDEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(21413..21703)
                     /locus_tag="WGR19_000653"
     CDS             complement(21413..21703)
                     /locus_tag="WGR19_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ0798223.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(21704..23272)
                     /locus_tag="WGR19_000654"
     CDS             complement(21704..23272)
                     /locus_tag="WGR19_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0798224.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQAQGYQYDPETDKFVKGYEYSNCLWYEDERRIN
                     RTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNN
                     FVLNRCTFDWNGGDCVVNKGDDYRNGANFSLSRNPKYKEEMDAKKLEEILSLKVDANP
                     DKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVI
                     PRPDLTPGSAEAPNAQPLPEVSPAENPANNPDPNENPGTRPNPEPDPDLNPDANPDTD
                     GQPGTSPDSPAVPGRTNGRDGKERKEGEDGGFLCRYFPKILACQEMGKPSDGMFHDIS
                     IPQVIDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYQASYEPLCVFAEKIRFAVLLAFI
                     IMSAFVVFGSLKGK"
     gene            complement(23214..>23447)
                     /locus_tag="WGR19_000655"
     CDS             complement(23214..>23447)
                     /locus_tag="WGR19_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0798225.1"
                     /translation="LIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDES
                     VIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
BASE COUNT         5874 a         6451 c         5485 g         5637 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaaaa acggctgcat atgttgtcta
       61 caaaatatcg ggaagcataa caggcaaaat gccgtctgaa atattttcag acggcatttc
      121 ttatccgaaa cggattattt ttgcgtttca accgcttcca atgcacgcag ggcataagtg
      181 taagcggcac cggcattcag ggcgatggcg gttgccaatg cgcctgcgat ttcgctgtcg
      241 gtcgcaccgg ctttggcggc agcggcggcg tgaacgctga tgcagctctc gcaacgagtg
      301 gtaatggcaa cggcgatggc aatcagttcg cgcgttttgg catcaagtgc ttcggcggcg
      361 gcggcctgtt ccaatgcgcc gtaggcctgc agcattttag gatgcgcctt acccagctcg
      421 ccgaacgatt ttttaaccaa tgcggtatgt tctttccaat ctttaaacat tttcttttcc
      481 tttctcttgc gtttaaccct gatacgcgct tgcgtatctg ttttcgatgt gcgtattatt
      541 gcaattattc agttgtgttt ctcgtttaat catctcattt tatggttcaa aaagatttat
      601 ggacattctg gacaaactgg tcgatttcgc ccaattgacg ggcagtgtgg atgtgcagtg
      661 ccttttgggc ggacaatggt cggtacggca tgaaaccttg caacgcgaag gattggtaca
      721 cattgttaca tcgggcagcg gctatctctg catcgacggc gaaacttccc cgcgtccggt
      781 cagtacaggg gatattgtat ttttcccgcg cggcttgggt catgtgttga gccacgacgg
      841 aaaatgcgga gaaagtttac aaccggatat acgacaaaac ggcgcattca cggtcaaaca
      901 atgcggcaac ggactggata tgagcctgtt ttgcgcccgt ttccgctacg acacccacgc
      961 cgatttgatg aatgggctgc cggaaaccgt ttttctgaac attgcccatc cgagtttgca
     1021 gtatgtggtt tcaatgctgc aactggaaag cgaaaaacct ttgacgggga cggtttccgt
     1081 ggtcaacgca ttgtcgtccg tcctgctggt gcttatcctg cgcgcctatc tcgaacagga
     1141 taaggatgtc gaactctcgg gcgtattgaa aggttggcag gacaaacgtt tgggacattt
     1201 aatccaaaag gtgatagaca aaccggaaga cgaatggaat gtcgacaaaa tggtggcggc
     1261 tgccaatatg tcgcgcgcgc aactgatgcg ccgtttcaaa agccgggtcg gactcagccc
     1321 gcacgccttt gtgaaccata tccgcctgca aaaaggcgcg ttgctgctga aaaaaaaccc
     1381 ggattcggtt ttgtcggtcg cactgtcggt aggctttcag tcggaaacgc acttcggcaa
     1441 ggcgttcaaa cggcaatatc acgtttcgcc gggtcaatac cggaaagaag gcgggcaaaa
     1501 ataaatcggg gcttcaaacg caaatgccgt ctgaaaaggc tttcatacgg catttgcgta
     1561 ccgcgtcatt tcaagggctg catcttcatc acttccatca aaaagttggt aaatgcgggg
     1621 ttgttgggtt tgacatccat atttttccaa cgctgctgcc agccgcgcaa ggcattctgg
     1681 atatacagct tggactgttc cgtattgatt gcgccccgct ggctgtctat cgccgaacgc
     1741 aggtagattt catacatact gtcatcgacg gcattgcgtc cgaccaggcg ttttctgaag
     1801 ttgttcagat attgcgccgc ctgaaccttg gtcattttac cgatacccac ctgatagccc
     1861 aagcgcgtcg cttcatcgct gattttggca acatccgtcc aatgcgaaga ggcaaggcgg
     1921 aaaccttttg caggtgcttc cgttttgacg gtattgatag gattcacggg gatttccgtc
     1981 aatgtgggca cataaataga ctggcagccg gaaagaactg ccacaatgga aagagggata
     2041 aggtattttt tcatgccccc attataatca agtttgcctt gagaaaacaa attgttcggc
     2101 aagaaaaata aaatttcggc atcagaagca ggcaaaaaca cattccacaa gccttgccgc
     2161 aaggtttaca atccgaccgt ccttatcgca acgaccgttt atggataccg caaaaaaaga
     2221 cattttagga tcgggctgga tgctggtggc ggcggcctgc tttaccatta tgaacgtatt
     2281 gattaaagag gcatcggcaa aatttgccct cggcagcggc gaattggtct tttggcgcat
     2341 gctgttttca accgttgcgc tcggggctgc cgccgtattg cgtcgggaca ccttccgcac
     2401 gccccattgg aaaaaccact taaaccgcag tatggtcggg acgggggcga tgctgctgct
     2461 gttttacgcg gtaacgcatc tgcctttggc caccggcgtt accctgagtt acacctcgtc
     2521 gatttttttg gcggtatttt ccttcctgat tttgaaagaa cggatttccg tttacacgca
     2581 ggcggtgctg ctccttggtt ttgccggcgt ggtattgctg cttaatccct cgttccgcag
     2641 cggtcaggaa acggcggcac tcgccgggct ggcgggcggc gcgatgtccg gctgggcgta
     2701 tttgaaagtg cgcgaactgt ctttggcggg cgaacccggc tggcgcgtcg tgttttacct
     2761 ttccgtgaca ggtgtggcga tgtcgtcggt ttgggcgacg ctgaccggct ggcacaccct
     2821 gtcctttcca tcggcagttt atctgtcgtg catcggcgtg tccgcgctga ttgcccaact
     2881 gtcgatgacg cgcgcctaca aagtcggcga caaattcacg gttgcctcgc tttcctatat
     2941 gaccgtcgtt ttttccgctc tgtctgccgc attttttctg gccgaagagc ttttctggca
     3001 ggaaatactc ggtatgtgca tcatcatcct cagcggcatt ttgagcagca tccgccccac
     3061 tgccttcaaa cagcggctgc aatccctgtt ccgccaaaga taaaaaatgc cgtccgaaca
     3121 tccttcagac ggcatatcgg gctttatttc cccgccttca catcctgcca ctggcgcacc
     3181 ataaacttca atgccgccgg ctggataggc accatgataa agctgttttt caaatcctcc
     3241 tcggttggga aaatcgtatt gtcgttttta aattcgtctt ccatcagctc acgcgccggc
     3301 ttgctcgaag gcgcataagt aacgaaattg ccgtttttcg ccgacacttc cgggtcgagg
     3361 aagtcgttga tgtatttgtg cgcgttggcg acgtttttcg catctttcgg aatcacgaaa
     3421 gaatccaccc aaatccccac gccctctttg ggcatcatca cgcggatttt ttccttgccg
     3481 cccgcttctt cggcacggcg tttggcgatg ttcaaatcgc cgccgaaacc gattgttacg
     3541 caggtatcgc cgcgcgccaa atcatcgata aagccggacg aagtaaagcg tttgatattg
     3601 gggcggtttt tcttgagcag ggcggttgcc tccctgatgt cttccgtatt gctgctgttc
     3661 gggtttttac ccaaatagtt caacaccata ggatagattt ccgccgcgct gtctaaataa
     3721 ctgataccgc attgtttgag tttggacgtg tattcggggt cgaacaccaa atcccactgg
     3781 ttgtccggca gcttgtccgt acccaaagcc tttttcacgc gttcggtatt gatggcgaag
     3841 gtatttgtcc cccaataaaa cggcacggcg tattcgtggc cgggatcgac cccgtccatc
     3901 agcctcatca tttcgggatt gaggtgttta taattgggaa tcagcgattt atcgattttc
     3961 tgatacgcac ctgccttaat ctgcctgccc acaaacgcat tggacggcgc gacaatgtcg
     4021 taaccggatt ttccggtcag caccttgctt tccagcgttt catcgctgtc gtacacatca
     4081 taagtaacct tgatgccgtt tttcttttca aaatcggcaa cggtttccgg atcgacgtat
     4141 tccgaccagt tgtaaatttt caatacgttt cggttttccg ccggcgccgg tttttcggca
     4201 ggcggtttgt ccgaaccgcc gcacgctgca agcagcaaag cagtcaggac ggcgagtggc
     4261 agatgtttgg ccattatcat tccttgcata tcgggttgga gaaagcggtc attatagccg
     4321 atattggcaa cagggcttca gacggcattc aaaatcccgc cacacccttc cgaaaaccgc
     4381 cgcttccata gctagaaaca gggatttgcg gtaagatacc gccgttcgtt ttccctgctt
     4441 ttaccatgac aagacatttg agagacattg aaaaaattat gaaaacctcc gaactgcgcc
     4501 aaaaattcct aaaatttttt gaaaccaaag gccacaccgt cgtccgctct tccagcctcg
     4561 tgccgcacga cgacccgacc ctgttgttta ccaacgcagg catgaaccag tttaaagacg
     4621 tatttttagg tttcgacaaa cgcccgtaca gccgcgccac caccgcgcaa aaatgcgtac
     4681 gcgcaggcgg caagcacaac gacctggaaa acgtcggcta caccgcccgc caccacacct
     4741 tctttgaaat gatgggcaac ttctccttcg gcgactactt caaacgcgac gccatccact
     4801 tcgcttggga atttctgact tcccccgaat ggctcaacat ccctaaagac aaactgttgg
     4861 cgaccgttta cgcggaagac gacgaagcct acaacatctg gttgaacgaa atcggtatgc
     4921 cgtccgagcg catcgtccgc atcggcgaca acaaaggcgc gaaatacgcg tccgacaact
     4981 tctggcaaat gggcgacacc ggcccttgcg gcccctgctc cgaaattttc tacgaccacg
     5041 gcgaagaaat ttggggcggc attcccggca gccccgaaga agacggcgac cgctggatcg
     5101 aaatttggaa ctgcgtattt atgcagttca accgcgacga acaaggcaat atgaatccgc
     5161 tgcccaagcc gtccgtcgat accggcatgg gcttggaacg catggccgcc gtcatgcagc
     5221 atgttcacag caactacgaa atcgacttgt tccaagacct gctcaaagcc gttgcccgcg
     5281 aaaccggcgc gccgttcagt atggaagaac ccagcctgaa agtcatcgcc gaccacatcc
     5341 gctcctgctc gttcctgatt gcagacggcg tcttgccttc caacgaaggc cgcggctacg
     5401 tattgcgccg cattatccgc cgcgccgtgc gccacggtta caaactgggt caaagcaaac
     5461 cgttcttcca caaactcgtt gccgatttgg tcaaagagat gggcggtgcc taccctgaat
     5521 tgaaagaaaa acaagcccaa atcgaagaag cattgaaaaa cgaagaaagc cgttttgccc
     5581 aaacgctgga aaccggtatg gctttgttgg aaaacgcgtt ggctaaaggc ggcaaaacac
     5641 tcgacggcga aatcatcttc aaactctacg atacttacgg tttcccatac gatttgaccg
     5701 ccgacatctg ccgcgaacgc aatatcgaac tggacgaagc aggcttcgag cgcgaaatgg
     5761 aagcccaacg cgcacgcgca cgcgccgccc aaagcttcaa agccaacgcc caactgcctt
     5821 atgacggtca agacaccgag tttaaaggtt atagcgaacg ccaaaccgaa tccaaagtcc
     5881 tcgccctcta caaagacggc gagcaagtcg acgaattgaa cgaaggcgac agcggcgcag
     5941 tcgtcatcga ctttaccccg ttctatgcag aatccggcgg ccaagtcggc gacgtcggct
     6001 atatcttctc aggcgaaaac cgctttgaag tacgcgatac ccaaaaaatc aaagcagccg
     6061 tattcggaca attcggcgta caaacttcag gccgtctgaa agtcggcgac agcgttaccg
     6121 ccaaagtgga cgacgaaatc cgcaatgcca atatgcgcaa ccacagcgcg acccacctga
     6181 tgcacaaagc cctgcgcgac gtattgggcg aacacgtcga acaaaaaggc tctttggtta
     6241 ccgccgaatc cacccgtttc gacatttccc atccccaagc ggtcactgcc gaagaaatcg
     6301 ccgaagtcga acgccgcgtc aacgaagcca ttttggcgaa cgttgccgtc aatgcagcca
     6361 ttatgagcat ggaagacgcg caaaaaaccg gcgcgatgat gctcttcggc gaaaaatacg
     6421 gcgacgaagt acgcgtactg caaatgggcg gtttctctac cgaattgtgc ggcggcacac
     6481 acgtttcacg caccggcgac atcggcctct tcaaaatcat cagcgaaggc ggtattgccg
     6541 caggcgtgcg ccgtatcgaa gccatcaccg gcctgaacgc actcaaatgg gcgcaagagc
     6601 aagagcgttt ggtgaaagac attattgccg aaaccaaagc ccaaaccgaa aaagacgtac
     6661 tggcaaaaat ccaagcaggc gcggcacacg ccaaagcatt ggaaaaagaa ttggcacgcg
     6721 ccaaagccga actcgccgtc cacgcaggcg ccaaactctt ggacgatgca aaagacttgg
     6781 gcgcagccaa actcgttgcc gcccaaatcg aagccgacgc agccgccctg cgcgaaatcg
     6841 taaccgacct gaccggcaaa tcagaacaag ccatcgtatt gctcgccgca gtcaacgagg
     6901 gcaaagtttc cctgtgcgca ggcgtatcca aagccctgac cggcaaagtg aaagcaggcg
     6961 atctggttaa atttgcagcc gaacaagtcg gcggcaaagg aggcggccgt cccgacttgg
     7021 cgcaggccgg cggcacggat gccggcaaat tgcctgaaat gctggcaagc gtggaaagct
     7081 ggctttgtca gaaattaagt taaatttaaa tcttaataag ccatttgagt cttgttcaaa
     7141 tggcttatat tttggattgt tagaatgaat actaaaacag agttacaaaa acttttagaa
     7201 gaggatatat caactttaac agaaacacta atttgtgcag atgctcttcc tccaagatat
     7261 gtacgtagca ttgcaactcc tattgtccgt cgatggttaa ttgacaaaca attaaatatt
     7321 ctcgcaaaag aaataggttt aactatagaa ttgcccatcc ttgatacaag tttggtattt
     7381 gaaaaattgt caaccttaga gaataaagtt aatttttata tggctggtgg agtttattta
     7441 ggcggagaat ttattagctc tatatatcat tcatcacaag aattttctgg tgaaccaata
     7501 atctatgctg aaccaaatat aattttatgt cctgctgaaa aatttttaac tttaaaacga
     7561 gttttccata atggaaatat ttttaatatg aatcagataa ttactttttt atccaataaa
     7621 caaggaggag tacattttga taaaaattac gataaatata aaacttggca agttgctata
     7681 gaaaaagcag caaatttttt aaaactcggt aatccatata atgaagataa attatctctt
     7741 agtgaagaac atgatactat tttagtagtt ttaccattag aaaaaggata tgaatggaat
     7801 tgtcttgaga tagaagtact atccgctgct caatctctag caaatatcta ttgcaacaaa
     7861 gttcgcctca tagatggtca tgtttggaag gaataaaaga aaaagcatag ttggtaattt
     7921 ttagttacgt agggtgggct tcagcccacc gttttttcat ttttgattgt tgattggtgg
     7981 gctaaagccc accctacggt tactaaaatc ccgtcattac ctcgcaggcg ggaatccggt
     8041 ttgttcggtt tcggtttttt agggacagtg ccgcaacatt aaatttccag attcccactt
     8101 tcgcacaaat aacagcgcaa atagggcaaa gataaggata tggggggttt tttctttgaa
     8161 tttactatac aattcgcccg tccccgttat agaaacccaa gccgatgaaa accctgctcc
     8221 aactccaaac cgccgcacaa aacttcgccg cctactacaa agaccaaacc gacgaacgcc
     8281 gcgagaaaga caccttctgg aacgaatttt tcgccatttt cggcatcgac cgcaaaaacg
     8341 tcgcccactt cgaatacccc gtcaaagacc ctgccgacaa cacccaattc gtcgatatat
     8401 tttgggaagg catcttcctt gccgaacaca aatccgccaa caaaaacctg accaaggcca
     8461 aagagcaggc ggagcgttat ttgcaggaaa tcgggcgcac caagccctcc gcgctgcccg
     8521 aatattacgc cgtcagcgat tttgcccatt tccaccttta ccgccgcgta cctgaagaag
     8581 gcgcagaaaa ccaatggcag ttccctttgg aagaactacc cgaatacatc acgcgcggcg
     8641 ttttcgactt catgttcggc atcgaagcca aagtccgcca aattcaagaa gaagccaaca
     8701 ttcaagcggc ggcggccatc ggcaggctgc acgacgcgct caaagaagaa ggcatttacg
     8761 aagaacacga gctgcgcctc ttcatcacgc gcctgctttt cctctttttt gccgacgaca
     8821 gcgccgtttt ccggcgcaac taccttttcc aagacttttt agaaagctgc aaagaagccg
     8881 acacgctcgg cgacaagctc aaccaactct ttgaattcct caacacaccc gaccaaaagc
     8941 gcagcaagac ccaaagcgaa aaatttaaag ggttcgaata cgtcaacggc ggcctgttta
     9001 aagaacgcct gcgcaccttc gacttcaccg ccaagcagca ccgcgcctta atcgactgcg
     9061 gcaatttcga ctggcgcaac atcagccccg aaatcttcgg cacgctcttc caatccgtca
     9121 tggacgcgca agagcggcgc gaagcgggcg cacactacac cgaagccgcc aacatcgata
     9181 aagtcatcaa cggctttttt tagaaaacct gcgcgccgaa tttgaagccg tcaaagccct
     9241 caaacgcgac aaagccaaaa aactcgccgc cttctaccaa aaaatccaaa acctgcaatt
     9301 cctcgaccct gcctgcggct gcggcaactt ccttatcatc gcctacgacc gcatccgcgc
     9361 ccttgaagac gacatcatcg ccgaagccct caaagacaaa gcaggcggcc tgttcgacag
     9421 cccgtccgtc caatgccgtc tgaaacagtt tcacggcatc gaaatagacg aatttgccgt
     9481 cctcatcgcc cgcaccgcca tgtggctcaa aaaccaccaa tgcaacatcc gcacgcaaat
     9541 ccgcttcgac ggcgaagtcg cctgccatac gctgccgctc gaagacgccg ccgaaatcat
     9601 ccacgccaac agcctccgta caccttggca ggcggcggac tacatcttcg gcaatccgcc
     9661 ctttatcggc tcgacctacc aaaccaaaga gcagaaaaac gacctcgaaa gcatctgcgg
     9721 ccatatcaaa ggctacggcc tgttggatta cgtctgcaac tggtacgtca aagccgccgg
     9781 catcatggca cagaatcccc aagttcagac ggcatttgtt tccaccaatt ccatctgcca
     9841 aggccagcag gtcgaaatcc tttggggcag ccttttgaag caaggcatcg aaatccactt
     9901 tgcccaccgc accttccaat ggacgagcca agccgcaggc aaagccgccg tccactgcat
     9961 catcgtcggc ttccgccaaa agccgcaaat gccgtctgaa aaaaccctct acgactatcc
    10021 cgacatcaaa ggcgaacccc aaaaacacgc cgcggccaac atcaatccgt atctgattga
    10081 cgcgcccgat ttgattattg ccaagcgcag ccgtcccata cattgcgaac ctgatatggt
    10141 caacggaagc aaaccgaccg aaggcggcaa ccttatcctt tcaaccgccg aaaaagatgc
    10201 cctgattgcc gccgagccct tggcggagca atacatccgc ccctttatcg gtgcggatga
    10261 gttcctcaac ggcaaaaccc gttggtgcct gtggtttcaa ggcatatccg acgttaaacg
    10321 caaccacgac ctgaaacaga tgcccaaagt tcaagcccgt attcaggcag tcaaaaccat
    10381 gcgcgaagcc agcagcgaca aacaaactca aaaagatgca gcaaccccgt ggctttttca
    10441 aaaaatccgc cagccttcag acggcaattt tctgattatc ccacgcgtct catccgaaag
    10501 ccgccgtttc atccccatcg gttatctatc atatgaaaca gttgtcagcg atttagtatt
    10561 tacccttcca aacgccaccc tctaccactt cggcatcctc agctccacca tgcacaacgc
    10621 ctttatgcgt accgttgccg gccgtctgga aagtcgttac cgctattcca ataccgttgt
    10681 gtacaacaac ttcccattcc ccgaaagctg ccggatgccg tctgaaaacg accgccaaga
    10741 cccgctccgc gccgccgtcg aaaccgccgc ccaagccgtc ctcgacgcgc gcggacaata
    10801 ccgccgagaa gcgcgggaag ccggtttgcc cgagccgacc ctcgccgaac tctatgcgcc
    10861 cgacgcaggc tataccgccc tagacaaagc ccacgccgcc ctcgacaagg cagtcgataa
    10921 agcctacggc tacaaaacag gcaaaaacac cgacgacgaa gccgaacgca ccgccttcct
    10981 gttcgagctg taccgcaagg cggcggcaat tgcgtagtag ttgcgtagta gttgcgtagg
    11041 atagttgcgt agggtgggct tcagcccacc gtttttcttc attttcggtt gttgattggc
    11101 aggctgaagc ccaccctgcg gttacaaaaa tcccgtcatt cccgcgcagg cgggaatcca
    11161 gacctttcgg ggcggaaact tatcggataa aacgatttcc cctgcatcct ggattcccac
    11221 tttcatggga atgacgggat ataggtttcc ctacggacgt gttcagattc ccgccttcgc
    11281 gggaatgacg gcggagaagt ttctgtcttt ccgataactt accgtaactt aaaatcccgt
    11341 cattaccgca aaagcagttg ctacggttac aaaaatcccg tcatccccac gaaagcgaaa
    11401 atcctgcctg tcggatttcg gttttttcgg gcgtttcggg aacttataaa tcgtcattcc
    11461 cgcgcaggcg ggaatccaga acgcgggacg gcggcaatat tcaaaggttg tctgaaaatt
    11521 cagaggttct agattcccgc ctgcgcggga atgacggcgg agcggtttct gcttttccga
    11581 taaatgaccg caacctaaaa cccatccttc ccacgaaaac agaaaaacaa aaacctaaaa
    11641 tcccgtcgtt ctcgcgaaag cagttgctac gtttaccgtc aaaaatgccg tctgaacgct
    11701 caagccttca gacggcattt tttatttcaa acccttacag gtagaatttt tcgaggactt
    11761 tcaggttgtc atccaatttg tacaccagcg gctgaccggt cgggatttcc aagcccataa
    11821 tgtcttcgtc ggaaatgccc tcgatgtgtt ttgccagcgc gcgcagggag ttgccgtgcg
    11881 ccgccaccaa gacgcgtttg ccgctcaaaa tcgcgggggc gatttggtct tcccaaaacg
    11941 gtaatacgcg ctccagcgtt actttcaggt tttcgccgtc gggtacgaca tcggcaggca
    12001 gatgggcata gcggcggtct ttgtgtgcgg aaaactcatc gtctttgtcc aaaagcggcg
    12061 gcagggtgtc gtagctgcgc cgccagatgc ggacttgctc gtcgccgtat tgttcggcgg
    12121 tttgtttttt gtccaaacct tgcagttggc cgtagtggcg ttcgttcagc cgccatgttt
    12181 tgatttgcgg tacgaacagt tggtcggatt cttccaaaac gatgttgcag gtcttaatcg
    12241 cgcgggtcag gacggatgtg aaggcgatgt cgaactcata gccgttttct ttcagttttt
    12301 taccggcggc ggcagcctcg gcaagcccct gctcgctcag cttcacgtcg cgccagcctg
    12361 taaacaggtt tttcgcgttc cattcgcttt gtccgtggcg gataaatacc agttccatat
    12421 cgtctccaat gtgtgaaagt gggaaagcct tatttataac atattttcac atttcccgta
    12481 tttgattcag attcagacac gcgcccacta tggtttgccg ttttgattta caataatgcc
    12541 ctttgcttta cattccgcat acacaatgtt tcaacacaca ggacgacaca taaagcaccg
    12601 ccctatgtgt tgccctgatt tggaaggggt tgccccctcc caaataaagt ctgattctac
    12661 cgccccgaag gacagatgtc caagtggcgg gggttcaacc gaaaaggaaa tacgataaat
    12721 atgcaaccgc acgctccaca taccgattcc aatacgctga tgctcggccg atacgccgaa
    12781 cgcgcctatc tcgaatacgc catgagcgtg gtcaaaggcc gcgcgctgcc tgaagtttca
    12841 gacggccaaa agcccgttca gcggcgcatt ttgtttgcca tgcgcgatat gggtttgacg
    12901 gcgggggcga agccggtgaa atcggcgcgc gtggtcggcg agattttggg taaataccac
    12961 ccgcacggcg acagttccgc ctatgaggcg atggtgcgga tggcgcagga ttttaccttg
    13021 cgctatccct taatcgacgg catcggcaac ttcggctcgc gcgacggcga cggggcggcg
    13081 gcgatgcgtt acaccgaagc gcggctcacg ccgattgcgg aattgctgtt gtccgaaatc
    13141 aatcagggga cggtggattt cgtgccgaac tacgacggcg cgtttgacga gccgctgcac
    13201 ctgcccgccc gcctgcctat ggtcttgctc aacggcgcgt caggcatcgc ggtgggcatg
    13261 gcgaccgaga ttccgtcgca caatttgaac gaagtgacgc aggcagcgat tgcgctgttg
    13321 aagaagccga cgctggaaac cgccgacctg atgcaataca ttcccgcccc cgattttgcc
    13381 ggtggcggtc aaatcatcac gccggcggac gaattgcgcc gtatttacga aaccggcaag
    13441 ggcagcgtgc gcgtgcgtgc acgttatgaa attgaaaaat tggcgcgcgg acaatggcgc
    13501 gtcatcgtaa ccgagctgcc gccgaacgcc aactccgcca aaatccttgc cgaaatcgaa
    13561 gaacaaacca acccgaaacc gaaagcgggt aagaaacagc tcaaccaaga ccagctcaac
    13621 accaaaaagc tgatgctgga tttaatcgac cgcgtgcgcg acgagtccga cggcgaacat
    13681 cccgtgcgac tggtattcga gccgaaatcc agccgcatcg ataccgatac cttcatcaac
    13741 acgctgatgg cgcaaacttc gctggaaagc aatgtgtcca tgaacttggt gatgatgggg
    13801 ctggacaacc gccccgcgca gaaaaacctg aaaacgattt tgcaggaatg gctggatttc
    13861 cgcaccgtaa ccgtaacacg ccgtctgaaa ttccgtttga accaagtgga aaaacggctg
    13921 cacatcctcg aaggccgtct gaaagtcttt ctgcacatcg acgaagtgat taaagtcatc
    13981 cgcgaatcag acgaccccaa aaccgacctg atggcggcgt tcgggctgac cgaaatccag
    14041 gccgaagaca ttttggaaat ccgcctgcgt cagttggcgc gtttggaagg tttcaaactc
    14101 gaaaaagaat tgaacgaatt gcgcgaagaa caaggccgtc tgaacatcct tttgggcgac
    14161 gaaaacgaaa aacgcaagct gattatcaaa gagatgcagg cggacatgaa acagttcggc
    14221 gacgcgcgac gcacgctggt ggaagaagcc ggacgcgccg tgttgacgca gaccactgcc
    14281 gacgaaccga tcacactgat tctgtcggaa aaaggctgga tacgcagccg tgccggacac
    14341 aatctcgatt tgagccaaac cgcgttcaaa gaaggcgacc gcctcaaaca aactcttgaa
    14401 ggccgcactg ttttgcccgt cgtcattctc gattcatcgg gcagaaccta cacgctcgat
    14461 gctgccgaaa tccccggcgg gcgcggcgac ggcgtaccgg tttcatcctt gatcgagttg
    14521 caaaacggcg cgaaacccgt tgcgatgctg acaggattgc cggaacaaca ttatttatta
    14581 tcgggcagcg gcggctacgg cttcatcgcc aaactgggcg atatggtcgg acgcgtgaaa
    14641 gcgggcaaag tggtgatgac cgcagacagc ggcgaaaccg tcctgccgcc ggttgccgtc
    14701 tatgcctcct cgttcatcaa ccccgactgc aaaatcattg ccgccaccaa tcaaaaccgc
    14761 gccctcgcct tccccatcgg cgaattgaaa attatggcga aaggcaaagg gctgcaaatc
    14821 atcggattaa acgccggcga atcgatgacg cataccgccg tttcttccga gctggaaatc
    14881 ctgattgaaa gcgaaggcag gcgcggcgca gcgcacaaag accgcatccc catctccctg
    14941 cttgaggcaa aacgcggcaa aaaaggcaaa ctattaccca tatcgggcag cctgaaacag
    15001 ctttcttccc cgaaataaac ccgactctgc acatactatg gtaatttcca atacccgcga
    15061 acttgagaaa ctcaaagacc ggattcccaa tctgatcaac atcgcccgca tcgccatcgt
    15121 cttccccctg ctgattatgc atatcttcgg gttggaggtc agcggacatg aaaacctgca
    15181 cgcttcgtgg acggcgtggg cgttttatgt ttggctcgcc attgcctgct ggctgatttt
    15241 cttttccatc atccatccgc attggcaatg gcaggctttg agaataccga gtttcagcgc
    15301 ggtggcggac atcacgatga tcggcgtgct gacctacctg ttcggcggca tcgactccgg
    15361 cttcggcatc ctgattctgc ccttcgtcgt ctgctcctgc ctgctcagct acgggcgtta
    15421 ccccctgctc tatgccagct acgccgccat cctgctgata ttgaatgccc ttgcagacag
    15481 caatatcaat atgtatccgc tgattctgga tgcaaaaacc gtcaccaaca ccttcgtcct
    15541 cgtggctggt tcctatttcg tcgccatgat tgcctcactg tcggtcagat acatcgaccg
    15601 tgccggaaaa ctcgcctacg acagccatat cgcctaccgg cgcatcaaag gcttgagcca
    15661 aaccgtactc aaccgcgttc aggaagcggt cgtcgtcatc aacgtcgagc atcagaccat
    15721 actgttcaat aaaaaggcaa aagatctgct ccccatgctt gaaatcggac agcataccgc
    15781 cctattcgac cctattaccg ttttatggga caaggcctct tcgcgtactt tcgaacgcaa
    15841 tatcgacacg cccgccctga ccgcccgcat ccgcgccgtg ccgatgaaca aagagcaaaa
    15901 caaactgctc atcctctaca tccgcccgca aaacgaaatt caggcagaag ccctgtccgt
    15961 caaacttgcc gcactcggac aactgaccgc caacctcgcc cacgaaatcc gcaaccccat
    16021 gtccgccatt cgccacgcca acgacctgct gcgcgaaaat atggaagcgg gggcggcaga
    16081 tccgttcaac gccaaattgt gcaaaatcat cgacggcaac gtctgccgta tcgacaaaat
    16141 gctcgaagat atttcctcgc caacaagcgc aacaaaaccg aacgcgaaac catcggcctg
    16201 ataccgtttt gggaagaatt caaacaagag ttcctgctcg gccatcccga tgccgccggc
    16261 tgcatccgcc tagatatgca gggcaatcac ctgactgcct atttcgatcc tgcccacctg
    16321 cggcaaatta tgtggaacct cgccaacaac gcgtggcggc acagccgcaa acagcccggc
    16381 tcgatttccg tcaccatccg ccccgcgcaa aaaaacaccg tctgcatcct ctttgccgac
    16441 cgcccgaagt gcaggaacac ctgttcgaac ccttttacac cacggcggaa aacggcaccg
    16501 gcctcgggct gtatgtcgcc cgcgaactgg cacatgccaa cttcggcgat ttgacctacc
    16561 tgcccgaagc caaatgtttc gaactcgcat taccggaaaa aaccaatgac tgaactgcaa
    16621 caccccgtcc tcgtcgtcga taacgaaacc gacattctcg acctgatgga aatgaccctg
    16681 atgaaaatgg gcttgcgcgt ccataccgcg tcaggcgttg ccgaagccaa aaacaagctc
    16741 gacagccaac gctattcgct cgtcctgacc gatatgcgta tgccggacgg ctcggggctg
    16801 gaagtcgtcc aacacatcaa cagccgcctg ctcgatacgc cggttgccgt catcaccgcc
    16861 ttcggcaacg ccgatcaggc acaggaagcg ttgcgttgcg gcgcgttcga ccccgatacc
    16921 atgcagatac aggactatct cgaccaaatc gaacgcgaca ttatcgaaca aaccctcaaa
    16981 caaaccgaag gcaaccgcac gcaggccgcc aaacgcttgg gcatcagctt ccgttccatg
    17041 cgctaccgta tggaacgcct caacatcggc tgacgacaaa acggcatccg caccatctcc
    17101 gcccacccga aaaaatgccg tctgaaacgg cacggcaaag cgggttcgcc ccacgcccga
    17161 acggacacaa aacaccatga ccgacatcct cattgacaac accgccaccg aaaccgtccg
    17221 caccctgata caggcattcc cccttgtgcc cgtttcccaa ccgcccgaac aaggcagtta
    17281 cctccttgcc gaacacgata ccgtcagcct caggcttgtc ggggaaaaaa gcagcgtcat
    17341 cgtcgatttt gcctccggcg cggcacaata ccggcgcaca aaaggcgggg gcgaactcat
    17401 cgccaaagcc gtcaaccaca ccgcgcaccc caccgtttgg gacgcaaccg caggattggg
    17461 gcgcgacagc ttcgtcctcg cctcgctcgg gctggccgtt accgccttcg agcaacatcc
    17521 cgccgtcgcc tgcctgcttt cagacggcat ccgccgcgcc ctcctcaatc ccgaaacgca
    17581 agacaccgcc gcgcgcatca acctccattt cggcaacgcc gcagtacaaa tgcccgccct
    17641 tgttcaaaca caaggcaaac ccgacatcgt ttatctcgac ccgatgtatc ccgaacgccg
    17701 caaaagtgcc gccgttaaaa aagaaatggc ctacttccac cggctcgtcg gcgaagcgca
    17761 agatgaagcg gcactcctgc ataccgcacg ccaaacagca aaaaaacgcg tcgtcgtcaa
    17821 acgcccccgc ctcggcgaac accttgccgg acaagcccct gcctaccaat acaccggcaa
    17881 aagcacccgc ttcgacgttt acctgcccta cgggacggac aagggataac gcccataaaa
    17941 caagacaccg aaaaatttgc cgttcttatg caaacgagaa accggttttt gcgtttcgac
    18001 tgttttggat aaatcatcac accttaaagt ttgtcattcc cacggaagtg ggaatccgat
    18061 tcattcagtt ttatagtggt ttaaatttaa accactatag ctgttttcga gtttcaggca
    18121 acttccaaac cgtcattccc acggaagtgg gaatctagaa atgaaaggca acaggaattt
    18181 atcgtaaatg accgaaaccg aacggactag attcccgcct gcgcgggaat gacggggcgg
    18241 gcggatgccg tctgaaattc cgtcattccc gtaaaaacgg gaatctagaa cttctgattt
    18301 ttcagacgac ttttgaacat tgccgctacc caatgatctg gattcccgcc tgcgcgggaa
    18361 tgacgagata ctactttaat tgacaacaga atctttacaa ttcattttca actcattgaa
    18421 tttcaatatt taaaaattca ctgaatttca gacacggcaa agcaggcatt taaaaaatgc
    18481 ctttttttcc tttcaggatt tacgccaatt tgtaacgcga tggatcgtaa tcttcgcctt
    18541 tcttgtgtac gtgatacgca ataacggcga gtttacgcat caatgcggcg atgattactt
    18601 ttttaggctt cttcttggct tccagtcttt tgatgaaatc gggaaatgcc cttatgcggt
    18661 atgcaaccat ggcaggcata aacaagacgg cgcgtaattt cctgttgcca aacttggtca
    18721 atttgccttt tcctcttacg cttgtacctg attctttttg ttgcggactc aatccggcaa
    18781 atgcagcaaa cttgtttgat gtttcaaacg ccgaagatgt cagatattga aacaatacgg
    18841 ctgcggtcat tcttccaatt cccggtatgg tttcaagaca tttcacgcct tccttgcatt
    18901 tcggcttctc cgtctgctct tttatcttcg cctttaaaac ttcaagctgt tcattcatgg
    18961 ctttgatgat ttctgcatat gctttagccg cttcttcatc ttttgccacg tgatgacggt
    19021 ttttcattgc cgcgcattcg cttttgattt gtgtatatgc tgcggtcatc cgtgaaagcc
    19081 tgtattgctc gtccgtaggc ttttgcctct ttacaagctc gctttcctgc gccgaccggc
    19141 aatactccgc tatcagtttt gcatcctgtt tgtctgtttt ggtgcgtttg aatctgcttt
    19201 ctgcatactt gcttattttc agcgggttca ctacgtaaac gctgtaatac tgcgcgaagt
    19261 agtcggcaac ttcttcataa tagtttcccg ttgcctccat acagatatgc agattctgac
    19321 atcccaagct tttcaaccag tccgaaaact gatttaaacc ttttgaattg ttatcaaact
    19381 ttgccgaata ttcggcattg ccgaccatgg cgaatgcgtc aaatgttagc tttgatatat
    19441 ccaatcctac ggcgttacgc atgagattac ccttatctat tcagaatcta tgttctttga
    19501 tactactcaa tttcacaaac aagaaaaccg cccgcctatt ctcgtcatca aactttaagt
    19561 ttgtggttta ttcaggctgg acggttgcgg caaagggtag ctattccttt gccgtgtctg
    19621 attttatttg ggttgcaggt tttggtaaag gtttctgttg cgacccgaat gtcttatttt
    19681 ttttgggcgt atctcagtct ggaatcactc cgttagtggg tttccggtat tgaaaaacag
    19741 ttcataaaaa aggaaaaggg ggtattcata aagattgggc aaaaagcgcg cccaatcttt
    19801 acaaagcttc ccccttttcc ttttttatga actattttcc tgcacctaca acccccgaac
    19861 gaagcgattc cagactgaga cactttaaaa aaaaacagcc attctagcag ttaaccccct
    19921 tcactcagct caagccatcc tgaggggtaa gggctgaaac cgaaagattt ttattttgaa
    19981 tgaaaggaac tttatgacat agattcaggc gcaaaaatca aagagcggtg gggataagca
    20041 aaaatcccca agcctgagaa caaggggatt ttttgcattc ctgccaagtg tgaaacttac
    20101 aggaaaatca aatgcagata actttgaaat ttggcaaact gcttttgact atcacgattg
    20161 atatacgttt tattctagcc gttatcatac tgataagtca ataacccacg acaagaaccg
    20221 cccaattaag ggcggttttc tatttaataa cgcctccgcc cgaaccttcg aatatcttgc
    20281 ccttctcctc ccaattatca tacatcaaat tttgctacgg ctttccgccc attacgagaa
    20341 cttgcggctt gtccgctttc gcggactgtt ccacctgttc cgtcctttgc tgttcgtcct
    20401 tataaggatt gaaaggcaac ccgtttttca catattcttt acacattatc tttgttattt
    20461 ctttcaaggg tgttccttga tttgaatagc atgtgcaatc tgattttccg ccgtctatac
    20521 atccggcgat ttgctcaaag gtttttacct gtctaacgtc gttgtaaatg ggcttacttt
    20581 cgggcttttc gggcaaagtc ggcgtaaagt cctcaggttt cagatttttg gaatgctcaa
    20641 aagtcgctgt ttctgatgat gccgtctgct ccgtcatcgt ctgttgcaca acgctttctt
    20701 tttgcgcttc ctgctcaatc tggccgcctg ttgctttact gtaaatttga aacatgccga
    20761 aacttttcca gcccacaaac cctacaatcg caatcaacgc ccaaaccgcc caaggtactt
    20821 ttttcttgaa cttttggtgc tggcttgctg atttatagta tttaaaggct tctttcggcg
    20881 gtttccaact tgcgacttct acgccgctta cgcctgcggg attgtccaac gaggttacgc
    20941 atttatacca ataatactgt ttcattccga ttgccttgcg ttcaaggtgt acatgctttg
    21001 aaacaaggtt gcggacgaat atatcaagtt ggctcgggtg ctgcgtcatc aaaataacgg
    21061 tatgcccgtg atggcggagt tctgtcagtt cctgaatata aggcggaacg ggacggcctg
    21121 ccgcgcgtac cggataagtg tagtgcgctt cgtcaacaat cagcactgcg ccttccggta
    21181 tgacatcacg aagcggggcg gacatgattt gctcttccgt cagttcgtgg gctttaaact
    21241 gccgtttatc caatccgtcg atatggcaga aataaagcgg cctgtctacc tctgtgccgt
    21301 cttccaattt cattttgaac aatccgtctt cgttgttcaa aatcatagag acgacgcggg
    21361 aggttttgcc tgtccccata tttcctgtaa acagataaat catgcttcta cctcatcctg
    21421 gaaagacaaa cgtcagtttt ttgaatgcgt gcataccaat gaagaacgag aatgcgccga
    21481 acaggtagcc caacccctga ccgaatcccg aaattaaaag aaggttcagt atgtcggaag
    21541 gcatggaatt gatcgcattt gacgtgtagt ctttgaactt ttccagcgcg atgagatacc
    21601 cggcataggt tacaaatgtc agacctgttg caaggattat tctgacaatc agcattttca
    21661 gaagtatgcc taaaagtgga atcagaccgg caagtaatgg catttatttc cccttcaacg
    21721 aaccgaaaac gacaaaagcc gacataatga taaaggcgag cagtacggca aaacggattt
    21781 tttcggcaaa cacgcataac ggctcatagc ttgcctgata ttgcctaccg aaaacatgaa
    21841 aggttttcgg ctgcggacat acgccgttag acggtaaaaa gttatgtgaa gaccatgttt
    21901 tatcgtctat aacctgcggt atgcttatat cgtgaaacat gccgtctgaa ggtttgccca
    21961 tctcctgaca ggctaggatt ttcgggaaat accgacataa aaatccgccg tcttcgcctt
    22021 ctttcctttc tttgccgtcc ctgccgtttg tgcgccccgg aacggcgggg gaatcggggc
    22081 ttgttccggg ctgtccgtcc gtatcgggat ttgcatcggg attcaaatcg gggtcgggtt
    22141 cgggattggg acgcgtgccg gggttctcat tggggtccgg gttgtttgcg gggttttcag
    22201 cgggcgatac ttcgggcagc ggctgtgcgt tcggcgcttc cgcgcttccg ggtgtgaggt
    22261 cgggacgcgg gattacttga acatccactg tggtgttgcc ttgcgaatcc ctgccgaatg
    22321 ttgcgacaac ctgaacggga ttcccgttcc tgtccgtgac gggccccata ttcacttttg
    22381 ttccgggtgc gacttctact ttttcggaat aaccgggata tccggttgcc tttatgtatt
    22441 tgtcgggatt ggcatcgact ttcaacgata aaatctcttc cagctttttg gcatccattt
    22501 cttctttgta tttcggattg cggctaaggg aaaaattagc cccatttctg taatcatcac
    22561 ctttattgac cacacaatct ccgccgttcc aatcaaatgt gcaacgattt aaaacaaaat
    22621 tattccaatc caaagaactt aatttattca gttcttcttt atgccaattc caaaacggac
    22681 gtgccagcct atacatttgg ctttccatca attctttgac ttcggggaat ctgctgtaat
    22741 cggacataag gcgcataatc gaactgtcaa cgccgtagca gccataggtt ctattaatac
    22801 gtctttcgtc ttcgtaccaa aggcaattac tatattcgta gccttttaca aatttgtcgg
    22861 tttcggggtc gtattggtag ccttgtgcct gtatgtcttc tttgaaagtt tcgtatacgt
    22921 cgtgggctaa aagggctgtt ccgacatagg gaaccgccct tgtgctgaat ttcgcgccta
    22981 agcgggcaag tttgccgacc cccgccaata cgccggcgcg ggaaacgctg gcggttattt
    23041 tggcgttgat tcgggctttt gcgcccgtag ggatgtgctc aaaatttgcc gcttctatga
    23101 atttggcaaa actcttagaa cttgaatcta atttaaaacc acttgatttt aaattattac
    23161 ttgattgaaa tttaatttgt gatctttcta atcttacagg ttcagaataa gaattactcg
    23221 aacatagaat taaaatcaga actatcggtg tactcttgat aaacgaattc atgaacagtt
    23281 tttccgttct cttctgattt tcttataaaa ataacagatt catcagaaaa ataaatcttc
    23341 caaatattat gtgcgactct cttatttaag aaataagaga aactctctaa aatatcgtac
    23401 tcttttacgt tattaacaaa ttcttcaaat tctttctgag caattaa
//
LOCUS       DAWFJW010000020        22556 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFJW010000020 DAWFJW010000000
VERSION     DAWFJW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.020"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(30..368)
                     /locus_tag="WGR19_000656"
     CDS             complement(30..368)
                     /locus_tag="WGR19_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0798226.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            463..675
                     /locus_tag="WGR19_000657"
     CDS             463..675
                     /locus_tag="WGR19_000657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798227.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            832..4818
                     /gene="purL"
                     /locus_tag="WGR19_000658"
     CDS             832..4818
                     /gene="purL"
                     /locus_tag="WGR19_000658"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0798228.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALNRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQAYGKPDHIASPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATGDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPYAT
                     EGNPFGKKPFNAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQ
                     TPVADCAVTMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCIGEAITNIAAVNIGDIG
                     NIKLSANWMAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADK
                     KSVVSPLSLIISAFAPVEDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYN
                     NMSGDAPDLDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLD
                     IDLNLLLAQTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVA
                     DIINLFYQQQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRL
                     RDNPACADSEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQ
                     IEMAAAFTRAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSIL
                     FHPALRDQFAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARL
                     SMVHVPKSASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQV
                     TQTYPLNPNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLF
                     AGARKALG"
     gene            4913..5671
                     /gene="gloB"
                     /locus_tag="WGR19_000659"
     CDS             4913..5671
                     /gene="gloB"
                     /locus_tag="WGR19_000659"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0798229.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            5985..10460
                     /gene="mspA"
                     /locus_tag="WGR19_000660"
                     /gene_synonym="ausI"
     CDS             5985..10460
                     /gene="mspA"
                     /locus_tag="WGR19_000660"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ0798230.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPILSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFVGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDMTGEAAIEKNRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYNLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPVT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSN
                     GNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALS
                     VNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            10692..12146
                     /gene="mgtE"
                     /locus_tag="WGR19_000661"
     CDS             10692..12146
                     /gene="mgtE"
                     /locus_tag="WGR19_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0798231.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDASLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDNLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(12232..13140)
                     /gene="hslO"
                     /locus_tag="WGR19_000662"
     CDS             complement(12232..13140)
                     /gene="hslO"
                     /locus_tag="WGR19_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0798232.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTAEELAGLDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            13385..14140
                     /locus_tag="WGR19_000663"
     CDS             13385..14140
                     /locus_tag="WGR19_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0798233.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLN
                     EQPVLPVNRVPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            14159..14377
                     /locus_tag="WGR19_000664"
     CDS             14159..14377
                     /locus_tag="WGR19_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798234.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            14522..14866
                     /locus_tag="WGR19_000665"
     CDS             14522..14866
                     /locus_tag="WGR19_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0798235.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            14866..15558
                     /locus_tag="WGR19_000666"
     CDS             14866..15558
                     /locus_tag="WGR19_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0798236.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTVVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            15625..16845
                     /gene="argJ"
                     /locus_tag="WGR19_000667"
     CDS             15625..16845
                     /gene="argJ"
                     /locus_tag="WGR19_000667"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0798237.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            16905..18314
                     /locus_tag="WGR19_000668"
     CDS             16905..18314
                     /locus_tag="WGR19_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0798238.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            18503..19894
                     /gene="hrpA"
                     /locus_tag="WGR19_000669"
     CDS             18503..19894
                     /gene="hrpA"
                     /locus_tag="WGR19_000669"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0798239.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     VAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKQRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRISAGV
                     CIRLFSEEDFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            19995..21122
                     /locus_tag="WGR19_000670"
     CDS             19995..21122
                     /locus_tag="WGR19_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798240.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANINEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            21300..>22556
                     /locus_tag="WGR19_000671"
     CDS             21300..>22556
                     /locus_tag="WGR19_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0798241.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWITGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGMLEVMLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEANIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNW"
BASE COUNT         5596 a         6932 c         5497 g         4531 t
ORIGIN      
        1 ctttcagacg gcatttccat tccaaacgct caaaccgccg cgtccgcacg ttcgcccgtg
       61 cggatacgga ttgcctcctc gaccggcagc acaaaaatct tgccgtcgcc gattttgccc
      121 gaacgcgcca cctcgacaat cacgtcaatc gcgcgttcca cagcgtcatc cgccaacacc
      181 agctcgattt tgaccttggg caggaaatcg acggcgtatt ccgcaccgcg atagatttcc
      241 gtatgcccct tctgcctgcc gaaccctttg acctcgctga cggtcatccc cgtaatgccg
      301 atttccgtca acgcctcgcg cacgtcgtcg agtttgaacg gtttgacaat cgcctcgatt
      361 tttttcatca cattctccat taattaacgt aaacaaacga caaatatagc agaaaccgcc
      421 ggcaaacgat aaacggtact gttcgcccgg cgatgcggcg atttgccgga aaccgtcatt
      481 cccgcgcagg cgggaatccc gatctctgca tttcagcaat atttaaaggt ttctgtaaat
      541 ccaaacttcc atattcctaa ccgtgcggaa acgacggcat ttgatctaaa aacatacgga
      601 agcagttgcc cgcccctcta cttcctgcaa ttatccgcat acccggcttt gggtaaaaat
      661 cagaaaaaca gataaaaccc ttattcctta atatcttcag acggcacctg tattttcccc
      721 aactgtatgc cgtctgaaga ttgtcaacat tttaagccaa aatatccaag caatacaaac
      781 ctgtccggcg tataatccct ctttttccca accgcatttg tgagccgaat tatgtctgtc
      841 gttttgccct tgcgcggcgt taccgccctt tccgatttcc gtgttgaaaa actcttgcaa
      901 aaagccgccg cactcggtct gcccgaagtc aaattaagca gcgaattttg gtatttcgtc
      961 ggcagcgaga aagcacttga tgccgcgact gtcgaaaaac tgcaagcctt actggcagcg
     1021 caaagcgttg aacaaacgcc aaaagcgcgc gagggcttgc atttgttttt ggtaacgccc
     1081 cgtttgggca cgatttcgcc gtgggcttcc aaggcgacca atatcgcgga aaactgcggt
     1141 ttggcaggca tcgaacgcat cgagcgcggc atggcggtgt ggctggaagg tgcgcttacc
     1201 gatgaacaga aacagcaatg ggcggcgttg ctgcacgacc gcatgaccga atccgtattg
     1261 cccgattttc agacggcatc caaattattc caccatctcg aatccgaaac cttctctacc
     1321 gtcgatgttt tgggcggcgg taaagaggct ttagtcaaag ccaataccga aatgggtttg
     1381 gcactctccg ccgacgaaat cgattatctg gttgaaaact atcaggcttt aaaccgtaat
     1441 ccgtccgatg tggaactgat gatgttcgcg caggcaaaca gcgaacactg ccgccacaaa
     1501 atcttcaacg ccgacttcat cctcaacggc gaaaaacagc cgaaatccct cttcggtatg
     1561 atacgcgaca cgcacaacgc gcatcccgaa ggcacggtcg tcgcttataa agacaattcg
     1621 tccgtgattg aaggcgcgaa aatcgagcgt ttctatccgg atgcggcgga aaaccaaggc
     1681 taccgtttcc acgaggaaga cacgcatatc atcatgaaag tggaaacgca caaccacccg
     1741 accgccatcg cgccgtttgc gggcgcggcg acgggcgcgg gcggcgaaat ccgcgacgaa
     1801 ggtgcgacgg gcaaaggttc gcgcccgaaa gcaggcttga ccggctttac cgtctccaac
     1861 ctcaacatcc ccgacctcaa acagccttgg gaacaagcct acggcaaacc cgaccatatt
     1921 gcttcccctt tagacatcat gatcgaaggc cccatcggtg gcgcggcgtt caacaacgaa
     1981 ttcggacgcc ccaacctctt gggctacttc cgcacctttg aagaaaaatt tgacggtcag
     2041 gttcgcggct accacaaacc gattatgatt gcgggcggct tgggcagcat tcaggcgcag
     2101 cagacgcata aagacgaaat ccccgaaggc gcgttgctga tccaactggg cggcccgggt
     2161 atgcttatcg gcttgggcgg cggcgcggct tcttcgatgg ataccggtac aaacgacgct
     2221 tctttagact tcaactccgt gcaacgcggc aaccccgaaa tcgaacgccg cgcgcaggaa
     2281 gtcatcgacc gctgctggca gctcggcgac aaaaacccga ttatctccat ccacgacgtg
     2341 ggcgcaggag gtttgtccaa cgccttcccc gaactcgtca acgatgccgg acgcggcgcg
     2401 gtattcaagt tgcgcgaagt gccgttggaa gaacacggtt taacgccttt gcaaatctgg
     2461 tgcaacgaat cgcaagagcg ttatgtgttg tcgattttgg aaaaagattt ggatgccttc
     2521 cgcgccatct gcgaacgcga acgctgcccg tttgccgtag tcggcacggc gaccggcgac
     2581 ggccatttga aagtacgcga cgatttgttc tccaacaacc ctgtcgattt gccgttgaac
     2641 gttttgctcg gcaaaccgcc caaaaccacg cgcaccgaca aaacggttac gccgtatgcg
     2701 accgaaggga atccctttgg gaaaaaaccg tttaatgccg gcgatatcga cattaccgaa
     2761 gccgcctacc gcgttctgcg cctgcctgcc gtagcggcta aaaacttcct gattaccatc
     2821 ggcgaccgca gcgtcggcgg catgacccac cgcgaccaaa tggtcggcaa ataccaaacc
     2881 cccgtagccg actgcgccgt gaccatgatg ggcttcaaca cctatcgcgg cgaagcgatg
     2941 tctatgggcg aaaaaccggc cgtcgccctg tttgacgcgc ctgcttcggg cagaatgtgt
     3001 atcggcgaag ccatcaccaa catcgcagcg gtcaacatcg gcgacatcgg caatatcaaa
     3061 ctttctgcca actggatggc ggcgtgcggc aacgaaggcg aagacgaaaa actctaccgc
     3121 accgtcgaag cggtttccaa agcctgtcag gcattggatt tgagcatccc cgtgggcaaa
     3181 gacagcctgt cgatgaaaac cgtttggcag gatggcgcgg acaagaaatc cgtcgtctcg
     3241 ccattgagcc tgattatctc cgcgttcgcg ccggtggaag acgtacgcaa aaccgttacg
     3301 cccgaactga aaaacgtcga agacagcgta ttgctgttta tcgatttggg cttcggcaaa
     3361 gcgcgtatgg gcggctcggc gtttggtcag gtgtacaaca atatgagcgg cgacgcgccc
     3421 gatttggacg atacaagtcg tctgaaagcg ttttacaacg tgattcagca gcttgtcgcc
     3481 gaagacaaac tcttggcgta tcacgaccgc agcgacggcg gtttgtttgc cgttttggta
     3541 gaaatggcgt ttgcggggcg gtgcggcttg gatatagatt taaatttatt gcttgcacaa
     3601 acatttatta ccaaccatac cgctctgtct caatcattgc ggactgaaga ggtaaaagcg
     3661 ttggctgaat ggcaagaaac cattgcccgc acattattta atgaagagtt gggtgctgtt
     3721 atccaagtta gaaaacaaga tgttgccgat attatcaatt tattctatca acaacagctg
     3781 catcataatg tctttgaaat cggtacgtta actgatgaga acacgttaat catccgcgac
     3841 gggcaaacgc accttatttc tgacaaccta atcaaactgc aacaaacctg gcaagaaacc
     3901 agccatcaaa tccaacgcct gcgcgacaac cctgcctgcg ccgacagcga gtttgccctg
     3961 attggcgaca acgaacgcag cgcattgttt gccgacgtga agttcgacgt gaacgaagac
     4021 atcgccgcgc cgtttatcaa cagcggcgcg aaacccaaaa tcgccatcct gcgcgaacag
     4081 ggcgtaaacg ggcaaatcga aatggccgcc gcgttcactc gcgccggttt cgatgcctac
     4141 gacgtgcata tgtccgacct gatggcaggc cgcttccgcc ttgccgactt caaaatgctg
     4201 gcggcgtgcg gcggcttcag ctacggcgac gtactcggcg cgggcgaagg ctgggcgaaa
     4261 tccatcctgt tccaccctgc cttgcgcgac caatttgccg ccttctttgc cgatccggac
     4321 acgctgacat tgggcgtgtg caacggctgc cagatggtca gcaacctcgc cgagattatc
     4381 ccgggtgcag aggcatggcc taaattcaaa cgcaacctga gcgaacagtt tgaagcacgc
     4441 ctgagcatgg ttcacgttcc gaaatcagcg tcgctgattc tgaacgaaat gcaaggctcc
     4501 agcctgcctg tcgtggtcag ccacggcgaa ggccgcgccg acttcgcgct tcacggcggc
     4561 aatatttccg ccgatttggg catcgcgctg caatatgtgg acggtcaaaa ccaggttacc
     4621 caaacctatc cgctcaaccc caacggctcg ccgcaaggca tcgccggcat taccaacgcc
     4681 gacggccgcg tgaccatcat gatgccccac cccgaacgcg tgtaccgcgc cgcgcaaatg
     4741 agctggaaac cggaaggctg gacggaactg tccggctggt accgcctctt tgccggcgca
     4801 cgtaaagcct tgggctaacc gccctactca aaccaatgcc gtctgaagaa tatttcagac
     4861 ggcgttccgg cataccatcc tttaaacggt atccgtccac cgaggaacac tcatgaaaat
     4921 cacccccgtc aaagccctaa ccgacaacta catctggatg atacagcacg gcaaccatgc
     4981 cgtctgcgtc gacccttccg aaccctcgcc cgtcttagaa ttcctcgtcc gcaaccgcct
     5041 catgcttgcc caaacatggg taactcaccc ccaccccgac cacgagggcg gtgcggcggc
     5101 actctggcgc ggctacatgg aatcgcccgt ttacggcgaa tccgacatcg aagcagcaac
     5161 ccacaccgta accgccggca cccaattcac cttcggcgac ggacaggtta ccgtttgggc
     5221 aacacccggc cacacagacc gccacaccag ctaccttctc gaaacttcag acggcataca
     5281 cgtcttttgc ggcgacaccc ttttttccgc cggctgcgga cgcgtgttta ccggcacaat
     5341 cgaacagctt tacgacagct tccaacgctt caaccgcctg cctgaaaaca ccctgttcta
     5401 cccggcacac gaatacaccg ccgccaacct gcgtttcgcc gcccatatcg agccggacaa
     5461 cgccgacatt cagacggcac tgaaagcggc ggcgcatacg cctaccctgc ccgttaccct
     5521 cgcgcacgaa cgccgcgtca atccgttttt gcgcgtcgac ctgccgcacg tcagagaccg
     5581 cgccgaggca ttgagcggga aaacgttaaa cagcagcctc gatacctttg tcgcgctgcg
     5641 tgaacttaaa aaccaatacc ggacgaaata aaacaatggg aaaacgcagc cattcctagg
     5701 atttttatta aaatcttaaa taaaatcata caatcatcgc caatagacga aaggacaccg
     5761 ttgccttata atcaaacaaa aacaaaatat ataatatagt ggattgaatt taaatcagga
     5821 caaggcgacg aagccgcaga tagtacggca aggcgaggca acgctgtact ggtttttgtt
     5881 aatccactat attgttaatc cactatataa atccagcaca aaacgggatc ggtgattctt
     5941 gtccgcaaga atcgttgatt ttctctatta cacggataat catcatgcgc ttcacacaca
     6001 ccaccccatt ttgttccgta ttgtccaccc tcggtctttt tgccgtttcc cctgcttact
     6061 catccattgt ccgcaacgat gtcgattacc aatattttcg cgactttgcc gaaaataaag
     6121 gcgcgttcgc cgtaggtgca agcaatattt ccatccaaga caagcaaggc aaaatattag
     6181 gcagggttct caacggcatc cccatgcccg acttccgagt cagcaaccgg caaaccgcca
     6241 tcgccaccct gattcacccc caatacgtca acagtgtcaa acacaacgtc ggctacggtt
     6301 ccatacaatt cggcaacgac acccaaaatc cagaagaaca agcctatacc taccgcctcg
     6361 tatcacgcaa cccgcacccg gactacgact accaccttcc ccgcctcaac aaactggtta
     6421 ccgaaatctc acctgccgca ctcagcagcg tacccttgct tggaaacggc cagccaaagg
     6481 ccaatgccta cctcgatacc gaccgcttcc catactttgt acgactcggc tcaggcacgc
     6541 aacaagtccg caaagcagac ggcacgcgta cacgaaccgc cccggcatac caatacctga
     6601 ccggcggcac gccgctgaaa gtattggggt tccaaaacca cggcttactc gtcggcggca
     6661 gcctgaccga ccaacccctt aacacctacg caatcgccgg agacagcggt tcccccctgt
     6721 ttgccttcga caagcatgaa aaccgctggg tgcttgcggg cgtactcagc acctacgccg
     6781 gcttcgataa tttcttcaac aaatacatcg tcacgcaacc cgaattcatc cgttccacca
     6841 tccgccaata cgaaacccgg ctggatgtcg ggctgaccac caacgaactc atatggcgcg
     6901 acaacggtaa tggcgacagc accctgcaag ggctcaacga acgcatcacc ctgcccattg
     6961 caaacccttc gcttgcccca caaaacgaca gcaggcacat gccgtctgaa gatgccggca
     7021 aaacgctcat cctatccagc aggttcgaca acaaaacact gatgctggca gacaatatca
     7081 accaaggcgc aggcgcattg cagttcgaca gcaacttcac cgtcgtcggt aaaaaccaca
     7141 catggcaagg tgcaggcgtt atcgtagccg acggcaaacg cgtcttctgg caagtcagca
     7201 accccaaagg cgaccggctc tccaaactgg gcgcaggcac gcttatcgcc aacggacaag
     7261 gcatcaacca gggcgacatc agcatcgggg aaggcactgt cgtactcgcc caaaaagctg
     7321 cttcagacgg cagcaaacaa gcattcaacc aagtcggcat caccagcggc aggggcacgg
     7381 ccgtcctcgc cgacagccag caaatcaaac ccgaaaacct ctatttcggc ttcaggggcg
     7441 gacggctcga cctcaacggc aacaaccttg cctttaccca tatccgccat gcggacggcg
     7501 gcgcgcaaat cgtcaatcac aaccctgacc aagccgcgac actgacgctg accggcaacc
     7561 ccatcctcag tcccgagcat gtcgagtggg tgcaatgggg caaccgtccg caaggcaacg
     7621 cggcggttta cgaatacatc aacccgcacc gcaaccgtcg gaccgactac ttcatactca
     7681 aacccggcgg caacccgcgc gaatttttcc cgttaaatat ggaaaactca acaagctggc
     7741 aatttgtcgg caacaacagg caacaggccg ccgaacaagt cgcccaagcc gaaaatgccc
     7801 gccccgacct gattaccttc ggcggatact tgggtgagaa cgcgcaaacg ggcaaagccg
     7861 caccgagtta cagcaaaacc aatatcgaat gggtgaatgg tccaaacacc ccacacaagc
     7921 cctcgcccta tacgaatacg ccaacccgca caggggcagg cgtaccgacg attaccgcct
     7981 caaagaaggc ggcaaccccg gcgcctactt cccatggaat gcctacagca cccaagactg
     8041 ggaatttctc ggacatgacc ggcgaagcag ccatagaaaa aaaccgccat atcgcaaatg
     8101 ccgccgtata cggccggccc gaataccgtt acaacggcgc actcaacctg cactatcgtc
     8161 ccaaacgcac cgacagcacg ctgttgctca acggcggcat gaaccttaac ggggaagtct
     8221 tgattgaggg cggcaatatg attgtgtcag gcaggcccgt accccatgcc tacgaccacc
     8281 aggccaaacg cgaacccgtt cttgaaaacg aatggaccga cggcagcttc aaggctgcac
     8341 ggttcaccct acgaaaccat gcccgactga cagcagggcg caataccgcg catctggacg
     8401 gcgacataac cgcatacaat ctgtccggca tcgacctcgg ctttatccaa ggcaaaacac
     8461 cggaatgcta ccgctcctac catagcggca gcacccactg cacacccaac gccgttttaa
     8521 aagccgaaaa ctatcgtgca ctacctgtaa cgcaagtacg cggcgacatt acccttaacg
     8581 accgttcaga gctccgcctg ggcaaagcac acctgtacgg cagcatccgt gccggcaaag
     8641 acaccgcagt ccgcatggaa gcagacagca actggacact ttcccagtcc agccacaccg
     8701 gcgcactgac gcttgacggc gcacaaatta ccctgaaccc cgatttcgcc aataatacac
     8761 acaacaaccg cttcaacaca ctgaccgtca acggcacact tgacgggttc ggcacattcc
     8821 gattcctgac cggcatcgtc cgaaaacaaa atgccccccc cctcaaactg gaaggggaca
     8881 gccgcggcgc attccaaatc cacgtcaaaa acaccggaca agaacctcaa acaaccgaat
     8941 cgcttgcact tgtgagcctc aatccgaaac acagccacca agcccgattc accctccaaa
     9001 acggctatgc cgatttgggt gcctaccgct acatcctccg caaaaacaac aacggataca
     9061 gcctgtacaa cccgctcaaa gaggccgaac ttcaaattga agccacgcgt gcggaacatg
     9121 agcgcaacca acaggcatac aaccaattac aggcaaccga catcagcaga caggttcaac
     9181 atgactctga cgcgaccagg caggcactac aggcctggca gaacagtcaa accgaacttg
     9241 cccgcatcga cagccaagtc caatatctgt ccgcccaatt gaaacagaca gacccgctga
     9301 ccggcattct gacgcgtgcc caaaacctgt gtgccgcaca aggatacagt gtcgatatct
     9361 gccgtcaggt tgccaaagcc gccgacacga acgacctgac actcttcgaa accgaactgg
     9421 atacgtatat agaacgtgta gaaatggccg aatccgaact tgacaaagca cggcaaggcg
     9481 gcgatgcgca agccgtcgaa acagcccggc acgcctacct gaacgcactc aaccgtctgt
     9541 cccgacaaat ccacagtttg aaaaccggcg ttgccggcat ccgtatgccg aacctggccg
     9601 aactgatcag ccggtcggcc aacaccgccg tttccgaaca ggccgcctac aataccggcc
     9661 ggcaacaggc gggacgccgc atcgaccgcc accttaccga tccgcagcag caaaacatct
     9721 ggctggaaac cggtacgcaa caaaccgact accatagcgg cacacaccgt ccctaccaac
     9781 aaactaccaa ctatgcacat atcggcatcc aaaccggcat caccgaccgt ctcagtgtcg
     9841 gtacgatttt aaccgatgag cgcacaaaca accgttttga tgaaggcgta tccgcccgaa
     9901 accgcagcaa cggcgcacat ctgttcgtca aaggggaaaa cggcgcactc tttgccgcgg
     9961 cagatttagg ctacagcaac agccgtaccc gatttaccga ttatgacggg gctgccgtcc
    10021 gccgccacgc atgggatgca ggcatcaaca ccggcatcaa aatcgatacc ggcatcaacc
    10081 tcagacccta tgccggcatc cgtataaacc gcagcaacgg caaccggtac gtactcgacg
    10141 gcgcagagat aaacagcccg gcgcaaatcc aaaccacatg gcatgccggc atccgtctcg
    10201 ataaaaccgt cgaactgggt caagccaagc tgacccccgc tttcagcagc gattactacc
    10261 atacccgcca aaacagcggt tccgccctca gcgtcaacga ccgtacctta ctgcagcaag
    10321 ccgcccacgg cacactgcat accctgcaaa tcgacgccgg atacaaaggc tggaacgcca
    10381 aacttcatgc cgcttacggc aaagacagca acaccgcccg ccacaaacag gcaggaatca
    10441 aaataggcta caactggtaa caagccgata aaaatgccgt ctggaacccg cgtttcagac
    10501 ggcatttgcg ttaaaaatag taaaccgttc caaaagggag tagaatagtg ccgtttccaa
    10561 ccctgcgcct gtaccgtcag gcttttatta tggaccttcc cagttcgttt ttactgaaca
    10621 ccccttccga ttccaaacga caataaccat cccgccggaa tgcctccccg cgcacggcgg
    10681 gcggagcatt tatgagcatc gaaccaaccc ctccgaacct tgaaaacgac ggtatcgaaa
    10741 acgatgtaga acgcgtttcc gccgatttcg accgtgtcca ctccctctgc gaaatcctcg
    10801 aacctgcttt tgaacaaatc gaaaacggca caccgctcga agacgcgtcg ctgcgcgaca
    10861 agctgaccga gctgaccgtc ctcttgagcg agctgcaccc tgccgacgtg gcggcggtat
    10921 tggaatcgct gccgccgcgc gaacgcaata tcgtctggat tctggtcaaa ccggaagacg
    10981 acggcgaagt cttgctggaa gtgtccgacg cggtgcgcga aacgctgatc gagtcgatgg
    11041 acaaagacga attgttggcg gcagttgaca atttggacgc ggacgaattg gcggaactgg
    11101 cagacgattt gccgcaccaa gtggtttatg aagccttgca gacgcgcgac gaggaagaac
    11161 gcgcccaagt caaagcggca atgtcctacg aagacaacca agtcggtgcg attatggact
    11221 tcgagttggt cagcatccgc gccgatgtcg cctgcgaagt ggtgctgcgt tacctgcgcc
    11281 gcttcgacag cctgcccgac cataccgaca agatttttgt cgtcgatgaa aacgacgtgc
    11341 tgcaaggcgt gctgcccatc cgcaaacttt tggtcgccga ccccgaagac ttggtggaaa
    11401 acgtgatggc gaaagatgtc gtgcgtttcc gcgccgaaga cgacgtggaa gaagcggcgc
    11461 aggcattcga acgctatgac ttggttaccg cgcccgtcgt cgatgaaaac aaaaagctca
    11521 tcggcagggt gaccgttgac gaaatggtgg acgtgatccg cgaagaatcc gaagcggata
    11581 tgttgaacat ggcaggtttg caggaagagg aagacctgtt cgcccccgtc ttggattccg
    11641 tcaaaaaccg ctggatgtgg ctcgccgtca acctctgcac cgccttcctt gccagccgcg
    11701 tcatcggcgc gtttgaaggc agcatcgaaa aaatcgtcgc actcgccgcg ctgatgccca
    11761 tcgtcgccgg tatcggcggc aactcgggca accagacgat taccatgatt gtccgcgcca
    11821 tggcgatggg gcaactgacg gatatgcagg cggggcgttt gctgaaaaaa gaagtcggcg
    11881 tcgccttggt caacggcatc atttggggaa cggtcatggg cgcagtatct tggctgcttt
    11941 acggcagcct cggcatcggt ttggtcatgg ttgccgcgat gacgctcaac ctgctgctgg
    12001 cggcaaccgt cggcgtatta attcccgtgg taatggaaaa gttcggacgc gatcccgcgc
    12061 tgggcagctc ggtgctgatt acggcggtaa cggattccgg cggcttcctg attttcttgg
    12121 gtctcgccac cctgttcctg ctttaaatgc cgtctgaacc cgcgcaaaaa tgccgtctga
    12181 agcggaagct gcttcagacg gcatttgact atttatcctt gttgcacaag attattggac
    12241 ggtatgccgg ggcagccctt tggcaacgcc gaccacatcc tccccgaaca gcgcgttgac
    12301 atcggtttcg tcaaacacat atttgctgtg gcagaaatcg caatcgactt cgatgctgcc
    12361 ttgttccacc accacgccgc cgacttcttc cccgcccagc atcaacagca tatcgctgac
    12421 tttgccgcgc gaacaggtgc atgaaaattc aaacgtttcc ggctcgaaca cgcgcggcgg
    12481 cgtttcgtgg aacaggcggt ataaaacgtg ttgcgcgtcc agtcctgcca gctcctccgc
    12541 cgtcagcgtg cgcgccagcg tactgacgtg ttcccatgcc tcttcatcca acaccgcttc
    12601 aggcagacgc tgcaccagca gaccgcccgc cgcttcgtcg cttgcagaca ggacgatgtg
    12661 cgtatcgatt tgttcggaac gcttcatata gttcgccaac atttgcgcga taccgccgcc
    12721 ttccaaaggc actacgccct gccagggttc gccgtctttg ggctgcagcg tcagcacgaa
    12781 tacgccgccc tcgcccaaaa ggtcgccgag gctttcgtca tcggctattt ctgcggtttc
    12841 gtcccaacgc gcggttgcac ggacggtacg gtcggaagcc gcttccgcaa ccagcatttt
    12901 cagccgcccc cgcccctgaa cctgcacaat cagcgtgcct tcgtttttga ggttgcccga
    12961 cagcaacaca cccgccgcca acaactcacc caaagcgcgg cggatggcgg cgggatagtt
    13021 tttctgtttt acaatgtgct gccatacgtt ttccagacgg acgtgcagcc cgcgcacggg
    13081 catatcgtcg aagataaagc gggtacgcac atcggcgcgg ttgatggcgg tttgattcat
    13141 gattttctct gactgattgt tcggatggcg gctatatggt tgcggtcggc gcgaaaacaa
    13201 gacggacggc ggatgcgctt cccaaattat caataaatta tataaaaatc aacatattaa
    13261 ctcaatctaa caagccgttt tttgccaaac agccgttttt ttatatacaa tcaacaagat
    13321 attttcgact gatacagcat aacatcgcac ggcggcacga tgcctcctgc gcggaaacac
    13381 cgatatggat tcttttttca aaccggcagt ttgggcggtt ttgtggctga tgtttgccgt
    13441 ccgccccgcc cttgccgacg agttgaccaa cctgctcagc agccgcgagc agattctcag
    13501 acagtttgcc gaagacgaac agcccgtttt acccatcaac cgagcccccg cccggcgggc
    13561 gggcaatgcc gacgaactca tcggcaacgc gatggggctt aacgaacagc ccgttttacc
    13621 cgtcaaccga gcccccgccc ggcgggcggg caatgccgac gaactcatcg gcagcgcgat
    13681 ggggcttaac gaacagcccg ttttacccgt caaccgagtc cccgcccggc gggcgggcaa
    13741 tgccgacgaa ctcatcggca acgcgatggg acttttgggt attgcctacc gctacggcgg
    13801 cacatcggtt tctaccggtt ttgactgcag cggcttcatg cagcacatct tcaaacgcgc
    13861 catgggcatc aacctgccgc gcacgtcggc agaacaggca cggatgggta cgccggttgc
    13921 ccgaagcgaa ttgcagcccg gagatatggt gtttttccgc acgctcggcg gcagccgcat
    13981 ttcccatgtc ggactttata tcggcaacaa ccgcttcatc cacgcgccgc gcacggggaa
    14041 aaatatcgaa atcaccagcc tgagccacaa atattggagc ggcaaatacg cgttcgcccg
    14101 ccgggtcaag aaaaacgacc cgtcccgctt tctgaactga tttcccaagg aatacgcaat
    14161 gagtatgccc gaaataccca aatggtacga cgatgacgga cagatcgtgt cctgtaccga
    14221 aaagatcaaa gtgatgtccg aaaatatggc cgagctgtat cagacggcac aagacgcgtt
    14281 tgaagacgcg ctgctgatgg gttgcggcga acgtcagttg cgcgattacc tgctcgcgct
    14341 gattgaaggt ttggaaaatc cctaccgcaa agtctgaaca cgccccggtt gctgcggcac
    14401 ggtttatccg tgccgttttt gcgtttgtgc gcggcttcgg cttttcagac ggcatatttg
    14461 acgttatgat taaacagtta acaagattta tcacaacgcc gtcaaaagac agacacacaa
    14521 catgaacatc atccgcgcgc tcctcatcat cctcggctgc ctcgccgccg gcgaaaccgc
    14581 cgttttccta gcaggcatca aactgcccgg cagcatcgtc ggcatgggcg tgctgtttgc
    14641 gcttttgcag gcaggttggg tcaaaacgtc ttggctacaa cagcttaccg acgcgctgat
    14701 ggcgaatctg acgctgtttc tcgtgccgcc ctgcgtggcg gtcatcagct atttggattt
    14761 gattgccgac gattggtttt cgatactggt ttccgcctcc gccagcactt tgtgcgtact
    14821 gctggttaca ggcaaggttc accgctggat acggagcatt atctgatgaa cgaaatcctc
    14881 aggcagccca gcgttctgct tttcctcacg cttgccgtgt acgcgcttgc gattatcgtg
    14941 cgcacgcgca cgggcaatat cttctgcaac cccgtactcg tcagcaccgt ggtgctgatt
    15001 gcctacctga aaatcctcgg tatcgattat gcggtgtacc acaacgccgc gcagtttatc
    15061 gatttctggc tgaaacccgc cgtcgtcgtg cttgccgtgc cgctctacca aaaccgccgg
    15121 aaaatcttca accaatggct gcccgtcatc gtttcgcaac ttgcaggcag cgttacgggc
    15181 attgttacgg ggatgtattt tgccaaatgg ctgggcgcgg aacgcgaagt cgtcctctcg
    15241 ctcgcatcca aatctgttac caatcccatt gccatcgaaa ttacccgctc catcggcggc
    15301 attcccgcca ttaccgccgc caccgtcatc attgccggtc tggtcggaca gattgccggt
    15361 tacaaaatgt tgaaaaacac agtcgtcatg ccctcgtccg tgggtatgtc gctcggcacg
    15421 gcttcgcacg cgatggggat tgccgcctcg ctcgaacgca gccgccgcat ggcggcatac
    15481 gcgggactgg ggctgacgtt caacggcgta ctgaccgcgc tgattgcgcc gctgctcatc
    15541 cccgttttgg gattttgaac ccgtttcaga cggcatttca gcccatgctg tctgaacgcc
    15601 gacacactcg caaggagaac cgttatggct gtcaacctga ctgaaaaaac cgccgaacaa
    15661 ctgcccgaca tcgacggcat tgccctctac accgcccaag caggcgtgaa gaagcccggg
    15721 cataccgacc tgacactgat tgccgtagcc gccggcagca ccgtcggtgc agtcttcacg
    15781 accaaccgtt tctgtgccgc gcccgtccac atcgccaaat cgcacctttt cgacgaagac
    15841 ggcgtgcgcg ccctcatcat caacacgggc aacgccaacg cgggtacggg cgcacagggc
    15901 agaatcgatg ctttggcagt gtgtgccgcc gccgcccggc aaatcggctg caaaccgaac
    15961 caggtgctgc ccttctccac cggcgtgatt ctcgaaccgc tgcccgcaga caaaatcatc
    16021 gccgcgctgc ccaaaatgca gcctgccttc tggaacgaag cggcacgcgc catcatgacc
    16081 accgacaccg tgcccaaagc cgcctcgcgc gaaggcaagg tcggcgacaa acacaccgtc
    16141 cgcgccacgg gcatcgccaa aggctcgggc atgattcatc ccaatatggc gaccatgctc
    16201 ggtttcatcg ccaccgatgc caaagtttcc caacccgtcc tccaactgat gacgcaggaa
    16261 atcgccgacg aaaccttcaa caccatcacc gttgacggcg acaccagcac caacgacagc
    16321 ttcgtcatca tcgccaccgg caaaaacagc caaagcgaaa tcgacaacat cgccgacccg
    16381 cgttacgccc aactcaaaga attgttgtgc agcctcgcgc tcgaactcgc ccaagccatc
    16441 gtccgcgacg gcgaaggtgc gaccaagttc atcaccgtcc gcgtcgaaaa cgccaaaacc
    16501 cgcgacgaag cccgccaagc cgcctacgcc gtggcacgtt cgccgctggt caaaaccgcc
    16561 tttttcgcct ccgaccccaa cctcggcagg ctgctcgccg ccatcggtta tgccggcgtt
    16621 gccgacctcg ataccgacct cgtggaaatg tatctcgacg atattttggt tgccgaacac
    16681 ggcggacgcg ccgcaagcta caccgaagca caagggcagg cggtaatgtc gaaggccgaa
    16741 atcaccgtcc gcatcaagct gcatcgcgga caagccgccg ccaccgtcta tacctgcgac
    16801 ctgtcgcacg gatacgtttc catcaacgcc gactaccgtt cctgacccga cacggcttca
    16861 gacggcatac ataaaattcc gtctgaaccg ccggacaaca taccatgacc tccacattcc
    16921 cccgccgcct cgcccgcaaa atccgccaaa cccgccgcct gtcgcgcaaa agcatcgcct
    16981 ttctgttcct tttggcaggt tcggcactcg tcgccctgac cgcgctgttt tttgcccatc
    17041 ttgccgattt tgcgctggaa ctgaacgcca aactggttca acaataccca tggttcgcgt
    17101 gggtcgcgct tcctttgggt ttgccgctta ttgcgtggct cacacgcaaa ttcgccccct
    17161 tcaccgccgg cagcggcatc ccgcaggtca tcgcctcact gtcgctgccc tacggcgcac
    17221 agaaaacgcg gctgatccgc ctcgggcaga cactgctgaa gattccgctg acctttttgg
    17281 gtatgctgtt cggcgcgtcc atcggacgcg aaggtccgtc cgtgcaggtc ggcgcggcag
    17341 tgatgggcgc gtggggcgcg tggtgcaaga aacacggctt ggcattcaaa gggatgcagg
    17401 aaaacgattt gatggcggcg ggcgcggcgg gcggtttggc agccgcgttc aacgcgccgc
    17461 tggcgggcgt gattttcgcc attgaggaac tcgggcgcgg catcatgttg cgctgggaga
    17521 ggcaaattct tttgggcgtg ctcgcctccg gtttcataca ggtcgccatt cagggcaaca
    17581 acccgtattt ttccggcttc aacggcggcg tattggaaca tatctttctg tgggtcgcac
    17641 tgtccggcct ggtttgcggc gcggcgggcg gcctgttcgg acgtttgctc tatcgcggtg
    17701 cggcggcgtt tgcaccgcgc aagatacgcg gcttcatccg caaccgtccg ctgctgctgg
    17761 cggcactgat ggggctgctg ctcgccctgc tcggcacgtt ctaccaaggc aaaacctacg
    17821 gcaccggcta ccacgaagcc gcccaagccc tgcacggcat ctatgaagcc cccttcggac
    17881 tcgccgccgc caaatggctc gccaccgtat tcagctattg ggcaggcgtt ccgggcggca
    17941 ttttcactcc ctcgctgacc ataggcgcgg ttttgggcga gcatatcgcc gccatcgccg
    18001 acatatcgca gggtgcaaac atcatcgtcc tcatctgcat ggcggcattt ctggcgggcg
    18061 cgacacaatc cccgattact tccgccgtcg tcgtcatgga aatgacgggc ggacaaagcc
    18121 tgctgttttg gatgctaatt gcctgcattt tcgcctcgca ggtttcgcgc cagttttcgc
    18181 cgcgtccgtt ctaccacgca tcgggaatgc gcttccgcca gcgcgtgctt caggaaacca
    18241 ccgcccaaac cggcaatgcg cccgcaagac cgcaaacagc aaacagcaaa acgggaatgc
    18301 cgtctgaaaa ttaaaacgcc cccgatcaaa cgccggcagc cgccttgatt tgaataccgt
    18361 tccgccgctg cttgaaactt caacaacaat gccgtctgaa cgacagaatg cggttttcag
    18421 acggcatttc cccatcccga tattgcctaa acaaaaccga agcgtttgct ataattgcac
    18481 tttttaccgc acacgcacgc ccatgtttcc cgatttctcc caaaccctct ccaaagaccg
    18541 ccacttcctg caatccgcct tcaaaaatcc caacaaatac ggcggcttgt ccaaagtcga
    18601 agaaaaatac cgaaaatcgc acgaaatctt tttgaaacgt ttggcagcct tgcccaaacc
    18661 cgaattcgac aacaccctgc ccgttcacga aaaacttgac gaaatcaaaa aaaccgttgc
    18721 cgaacatcag gtaacgatta tttgcggcga aaccggttcg ggcaaaacca cgcagttgcc
    18781 caagatttgt ttggaactcg ggcgcggggt agcgggtttg atcgggcata cccagccgcg
    18841 ccgtttggcg gcgcgttcgg tggcagagcg gattgccgaa gagctgaaat ccgaaatcgg
    18901 cagcgcggtc ggctataaag tacgcttcac cgaccacacc tcgcgcgatg cctgcgtcaa
    18961 gctgatgacc gacggcatcc tgctggcgga aacgcagacc gaccgttatc tcgccgccta
    19021 cgacacgatt atcatcgacg aagcgcacga gcgcagcctg aacatcgact tccttttggg
    19081 ctatttgaaa caactcctgc cgcgccgccc cgatttgaaa gtcatcatca cctcggcaac
    19141 gatagacgca gaacgcttct cccgacactt caacggcgcg cccgttttag aagtgagcgg
    19201 acggacgtat cccgtcgaaa tcctctaccg accgctgacc ggcaaagacg aagacgacgc
    19261 agaagtggag ttgaccgacg cgattgtcga tgccgccgat gaattggcgc gatatggcga
    19321 aggcgatatc ttggtattcc tgccgggcga acgcgaaatc cgcgaagccg ccgaagccct
    19381 gcgcaaatcc acgctgcgcc gcaacgacga aatcctgccc ctgttcgcac gcctgtcgca
    19441 cgccgaacag cacaaaatct tccacccctc cggcgcaaaa caacgcatcg tgctggcaac
    19501 caacgtcgcc gaaacctcgc ttaccgtgcc gggcatcaaa tacgtcatcg acaccggtct
    19561 cgcacgcgtc aaacgctatt ccgcacgggc gaaagtggag cagcttcata tcgaaaaaat
    19621 ctcccaagcc gccgcccgcc aacgctccgg ccgctgcgga cgcatctccg caggcgtatg
    19681 tatccgactg ttttcagaag aagatttcaa caaccgcccc gaatttaccg accccgaaat
    19741 cgcccgcagc aacctcgccg ccgtcatcct gcgcatggca tcgctgaaac tcggcgacgt
    19801 agcggcattc ccatttttag aaatgcccga ttcgaggtat attaatgatg ggtttcaggt
    19861 gttgctggaa ttaggggcag tggaggccgc ctgaaaacaa gtaggatata cacttctacc
    19921 tgacaaagac caacaaacga taaaactacg gcacatctag atgcccaacc tacaacattg
    19981 cacacggtaa aaacatgaaa cacttactca tcgattttga aaacgtccag ccgcaaaact
    20041 tagacaaatt actaaccgaa aatacccata tttggctatt tataggtgta ttacacaaaa
    20101 tgttacctat tagtctggtg caatccctac tacgtttcgg cgaacgtgtc catcttgtcc
    20161 agttacaaaa aacggggaaa aacgcattgg atttttacct gtcctattac ctcggacaaa
    20221 ttaccgccac agaccccaat gcccaaatcg gcatactctc gcgtgatgga ggatacgatg
    20281 ttctggtcga acatattttg aaaaaccacc aagcgaaggg tatcgtgcgc ctagccaata
    20341 taaatgaagt acaacatcag aaaattacta ccgaaccgcc gtcagcattg ctggaaaaca
    20401 ctcctcagcc tgaaaccaca ctcaaaccac agcaaccatt aacttcctat ttccaagcag
    20461 ccctaactgc actgcgccgc cccgacgctt tccgcccctg ccgcctgcat aacctgcgac
    20521 aaaatctgcg taagcatatt ttgagtgatt tgtttaaaga aaaaaccgat gaagaatgcg
    20581 aaataaccac tgctaacgtt atcaataaac tcaaagcaca aaacttcatc agcattgatg
    20641 aacaggaaac cgtttcctac catctcagtg ataatgattt gttacaaaga atccaacgcc
    20701 atattttaag ccaacgtccc aaaacctacg ctgattttca agccgtcgtg caaaaccgag
    20761 cagatgcact tcacttaaca gtcggtacca acgacattca atcctttgcg cgacatttgc
    20821 gcgaccaaaa cctgatccgc caaaacaatg ggaaaattga atatgcaccg tttactgaac
    20881 ctaaaccaca gccaacaccc aagcagccta aaaaaaccgc atgggaacct gatgaaatta
    20941 tttggaaaaa agtgattgcc gcgttatcgt taaagaaccg tcctaataaa accaaaactt
    21001 tacgcaatac aatccaggca ctcacaaaat ccaatgcaca agaaactgac aaactgctac
    21061 aacatttaca agatacccaa gtcctacgta ttgacggtac taaaattgtg tatatcaagt
    21121 aggatgggca tttatgcctg acctacaaaa ccaaaaacaa cctaacaccc ttaatcaact
    21181 ccacaaaccc ttttcagaca acctcgtttt ttgaaaaaca atctgtaaac agataactgc
    21241 tgaagaatac cgttgccgag ccccaaaacc cgtcctgcaa cttttattgt gaacttccca
    21301 ttatgaaaaa atcccttttc gttctctttc tgtattcgtc cctacttact gccagcgaaa
    21361 ttgcttatcg ctttgtattc ggaattgaaa ccttaccggc tgcaaaaatg gcagaaacgt
    21421 ttgcgctgac atttgtgatt gctgcgctgt atctgtttgc gcgttataag gcaacgcgtt
    21481 tgttgattgc ggtgtttttc gcgttcagca ttattgccaa caatgtgcat tacgcggttt
    21541 atcaaagctg gataacgggc attaattatt ggctgatgct gaaagagatt accgaagttg
    21601 gcggcgcggg ggcgtcgatg ttggataagt tgtggctgcc tgcgttgtgg ggcatgttgg
    21661 aagtcatgtt gttttgtagc cttgccaagt tccgccataa gacgcatttt tctgccgata
    21721 tactgtttgc cttcctaatg ctgatgattt tcgtgcgttc gttcgacacg aaacaagagc
    21781 acggtatttc gcccaaaccg acatacagcc gcatcaaagc caattatttc agcttcggtt
    21841 attttgtcgg acgcgtattg ccgtatcagt tgtttgattt aagcaaaatc cctgtgttca
    21901 aacagtctgc gccaagcaaa atcgggcagg gcagtgttca aaatatcgtc ctgattatgg
    21961 gtgaaagcga aagcgcggcg catttgaaat tgtttggcta cgggcgcgaa acttcgccgt
    22021 ttttaacccg gctgtcgcaa gccgatttta agccgattgt gaaacaaagt tattccgcag
    22081 gctttatgac ggcagtatcc ctgcccagct tctttaacgt cataccgcat gccaacggct
    22141 tggaacaaat cagcggcggc gataccaata tgttccgcct cgccaaagag cagggctatg
    22201 aaacgtattt ttacagcgcg caggcggaaa accaaatggc aattttgaac ttaatcggta
    22261 agaaatggat agaccacctg attcaaccga cgcagcttgg ctacggcaac ggcgacaata
    22321 tgcccgatga gaagctgctg ccgctgttcg acaaaatcaa tttgcagcag ggcaggcatt
    22381 ttatcgtgtt gcaccaacgc ggttcgcacg ccccatacgg cgcattgttg cagcctcaag
    22441 ataaagtatt cggcgaagcc aatattgtgg ataagtacga caacaccatc cacaaaaccg
    22501 accaaatgat tcaaaccgta ttcgagcagc tgcaaaagca gcctgacggc aactgg
//
LOCUS       DAWFJW010000021        22029 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFJW010000021 DAWFJW010000000
VERSION     DAWFJW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22029
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.021"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(162..1118)
                     /gene="gshB"
                     /locus_tag="WGR19_000672"
     CDS             complement(162..1118)
                     /gene="gshB"
                     /locus_tag="WGR19_000672"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0798242.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALEAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            complement(1211..2899)
                     /locus_tag="WGR19_000673"
     CDS             complement(1211..2899)
                     /locus_tag="WGR19_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0798243.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            complement(3011..3784)
                     /locus_tag="WGR19_000674"
     CDS             complement(3011..3784)
                     /locus_tag="WGR19_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0798244.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEGGSLKRHHGGASSGGNLPEGLSADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRVITNNIHVASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            complement(3827..4729)
                     /locus_tag="WGR19_000675"
     CDS             complement(3827..4729)
                     /locus_tag="WGR19_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0798245.1"
                     /translation="MDSLMTLISVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            complement(4878..5594)
                     /locus_tag="WGR19_000676"
     CDS             complement(4878..5594)
                     /locus_tag="WGR19_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0798246.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            complement(6163..6585)
                     /locus_tag="WGR19_000677"
     CDS             complement(6163..6585)
                     /locus_tag="WGR19_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0798247.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(6703..7374)
                     /locus_tag="WGR19_000678"
     CDS             complement(6703..7374)
                     /locus_tag="WGR19_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0798248.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(7390..8016)
                     /locus_tag="WGR19_000679"
     CDS             complement(7390..8016)
                     /locus_tag="WGR19_000679"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0798249.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGISTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(8123..8929)
                     /locus_tag="WGR19_000680"
     CDS             complement(8123..8929)
                     /locus_tag="WGR19_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0798250.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     TKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(9188..9628)
                     /locus_tag="WGR19_000681"
     CDS             complement(9188..9628)
                     /locus_tag="WGR19_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0798251.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(9691..11097)
                     /locus_tag="WGR19_000682"
     CDS             complement(9691..11097)
                     /locus_tag="WGR19_000682"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0798252.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHTAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIIALGHDVSGVMGNNQDLVDSLLAASRNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGSE
                     KGATGRPVPLLLNYLRNL"
     gene            11267..12382
                     /gene="lptF"
                     /locus_tag="WGR19_000683"
     CDS             11267..12382
                     /gene="lptF"
                     /locus_tag="WGR19_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0798253.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGRFNTLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGNDNIIFAKEGNFSLNDNKRTLELRDGYRYSGTPG
                     KADYNRVSFRHLSLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRGGHTYNILIAIGLFLIYQNGLTLLFEAVENGKIHFWLGLLP
                     MHIIMFAVALILLRVRSMPARPFWRAVAESLTPKGRK"
     gene            12379..13449
                     /gene="lptG"
                     /locus_tag="WGR19_000684"
     CDS             12379..13449
                     /gene="lptG"
                     /locus_tag="WGR19_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0798254.1"
                     /translation="MNLISRYIIRQMAAMTVYTLLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLATLIGGLLSLGRLAADSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            13759..16344
                     /gene="acnB"
                     /locus_tag="WGR19_000685"
     CDS             13759..16344
                     /gene="acnB"
                     /locus_tag="WGR19_000685"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0798255.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            16486..17481
                     /locus_tag="WGR19_000686"
     CDS             16486..17481
                     /locus_tag="WGR19_000686"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0798256.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(17550..17651)
                     /locus_tag="WGR19_000687"
                     /pseudo
     CDS             complement(17550..17651)
                     /locus_tag="WGR19_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17705..18718)
                     /gene="ilvC"
                     /locus_tag="WGR19_000688"
     CDS             complement(17705..18718)
                     /gene="ilvC"
                     /locus_tag="WGR19_000688"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0798257.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(18798..19091)
                     /locus_tag="WGR19_000689"
     CDS             complement(18798..19091)
                     /locus_tag="WGR19_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0798258.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(19158..19649)
                     /gene="ilvN"
                     /locus_tag="WGR19_000690"
     CDS             complement(19158..19649)
                     /gene="ilvN"
                     /locus_tag="WGR19_000690"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0798259.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(19660..21387)
                     /gene="ilvB"
                     /locus_tag="WGR19_000691"
     CDS             complement(19660..21387)
                     /gene="ilvB"
                     /locus_tag="WGR19_000691"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0798260.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDRKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
BASE COUNT         5107 a         5993 c         5684 g         5245 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata tataccgtct gaaaacgggg cggcggggtg
       61 tccgtacggc attaaccgca tccctgccgg ctgagggaaa accctgcctg cccgaacaaa
      121 ccaaacggtt gtgaagcaaa agcctttcag acgacatcgg tttaacgtac cgaccacgcg
      181 gcaacggcat cggcaaacat tgccgccacg tcgaaacctt tttgtttcat gatttcttgg
      241 aatccggtcg ggctggttac gttgacttcg gtcaggttgc tgccgataac gtccaaaccg
      301 gccagcagga tgccgcgccg tttgagttcg ggggcgagcg tttcggcaat ttcgcggtcg
      361 cgtccgccca attcctgcgc cacgccgcgc ccgcctgccg caaggttgcc gcgtgtttcg
      421 ccgttttgcg ggatacgcgc caaagcatag gggacgactt cgccgccgat aatcaggatg
      481 cgtttgtcac cgtgtacgat ttcgggaatg tagcgttgcg ccataatggt gcgggaatca
      541 agctgcatca gggtttcgag gatgctgccg atgttggggt ctttttcggt caggcggaag
      601 atgcccatac cgcccatgcc gtcgagcggt ttaatgatga tgtcgccgtg ttctttcaaa
      661 aatgcgcgga catcggcgga acgggtcgtt accagcgtgg gcgcggtaaa gcggctgaag
      721 ttcaaaatcg ccagtttttc attaaagtcg cgcatcgcct gtccgctgtt aaagaccttc
      781 gcgccctgct gttccgccag cgtcagtaat tgggtggcgt agaggtattg catatcgaac
      841 ggcggatcgg tacgcataat cacggcatca aatgcttcca atgccgtctg aactttgtcg
      901 gcggcggcaa accagttttt gtcgtggtcg tcttgtgcgc cgagaaactc aaaagcagac
      961 gcatcagcaa ccaccagacc gtttttaacc gacaattccc cgctcaaggt atgaaacagc
     1021 cgccaaccgc gttttgccat ctcgcgcatc atggcgtagg tggtgtcttt gtaggttttg
     1081 aaacttgcca tcgggtcggc gataaagagg actttcatca tatttccttt ccggtgtgcc
     1141 gaatgtgccg catttcgcgg gcaaagggga aatgccgtcc gaacaatatt cagacggcaa
     1201 ggatggggtt ttacttaggc tgccaagaat ctttcagcgt gaccgtgcgg ttaaacaccg
     1261 gcgtgtcttt gccgtggtct ttacggtcgg ttacgaagta gccgatacgc tcgaactgcc
     1321 aacggctttc tgccggcaaa tctttggcgg caggttcggc gtaggcggtg atttccttga
     1381 cggattccgg attgaggaaa tcggtgaacg gcaggtattc gccgtcttcg ccgcgcacgg
     1441 catcgggacg ctcgacggta aagaggcggt cgtacagacg gactttgatt tcggcggcgt
     1501 gttcggcgga aacccaatga atcacgcctt taactttacg gccttctgga tttttgccca
     1561 aggtgtcgtg gtcgatgctg catttgagtt caaccacatt gcctgcttcg tctttgacga
     1621 cttcatcgca cttgatgaca tagccgtggc gcaagcgtac ttcgccgccg ggaatcaggc
     1681 gtttgaagcc tttgggcgga ttttcggcaa agtcgtcggc ttcgatgtaa atggtttgtg
     1741 aaacaggtac ttcgcgatcg cccatttctt cgtggttcgg atggaacgcg gcacggcggc
     1801 tttgggtttt gccggcttca aagttggtca gggttacttt gagcgggttc aacaccgcca
     1861 tcaggcgcgg ggcggagttt tccagctctt cgcgaatcgc accctccaac acgctcatat
     1921 cgacgatgtt ttcagatttg gaaataccgg cgcgtttggc aaacaggcgc aggccttcgg
     1981 gcgtgtagcc gcgacggcgc ataccggaaa tggtcggcat acgcggatcg tcccagccgg
     2041 aaacgtgttt ttccacaacc aactgattca atttccgttt ggaggtaatg gtgtacaaaa
     2101 gctccaaacg ggaaaactcg tattggcgcg gacgtgtggc gtgcggcgcg gggatgttat
     2161 ccaacaccca gtcgtacagc ggacggtgcg cttcaaactc aagcgtacac aaggaatgcg
     2221 tgatgccttc gatggcatcg gaaatgcaat gcgtgtagtc gtacatcggg tagatacacc
     2281 atttgtcgcc ggtgttgtgg tgatgggcgc ggcggatgcg gtagatgacg gggtcgcgca
     2341 tattgatgtt gcccgatgcc atgtcgattt tcaggcgcag ggttttgctg ccgtcgggga
     2401 actcgccgtt tttcatgcgt gtgaacaggt cgaggttttc ttcgacgctg cggtcgcggt
     2461 aagggctgtt tttacccgct tcggtcagcg taccgcggta ttcgcgcatt tcttcgggcg
     2521 tcaaatcatc gacatacgct ttgccgtctt taatcaaacc gacggcgtag tcataaagct
     2581 ggtcgaaata gttggaagcg aaacgcggct cgcccgccca atggaaaccg agccactcga
     2641 catcttcttt gatggcgttg acgtattcgt cgttttcttt ttcggggttg gtatcgtcaa
     2701 aacgcaggtt gcacaagccg tcgtaaatat acgccaaacc gaagttcagg cagatggatt
     2761 tggcgtgtcc gatgtgcagg tagccgttgg gctccggcgg aaaacgggtt tggatggttt
     2821 cgtgtttgcc gcttttgagg tcttcttcga tgaaggtgcg gataaaatgg ttgtccgcaa
     2881 attggtcttt attgagcata gttttctttg aacagatggc ttcagacggc attggggcgg
     2941 ttccttatgc cgtctgaagc ggtttgatga tgtgtttatt gtacccgact tgcgcgcttt
     3001 gacatagcgt tcagacggca tcggcaatca ggcattccac ccccgcctct ttcagcatct
     3061 tctgcatcgc ggtatcgggc agccggtcgg taaatacttt gtcaaacgcc gtaatgtcgc
     3121 cgagcctgac cagcgcgttg ctgcggaatt tactgtggtc cacgccgagg aagcggacgc
     3181 gcgcattggc aatcatcgcc tgcatcacgc tgacttcttt gtagtcgtcg tccaaaagcg
     3241 aaccgtcgct ttccacgccg tgcgtactca tcacggcata atcgactttg aactggttga
     3301 taaaatcgac ggttgccacg ccggtaatgc cgccgtccaa agggcggacg actccggaag
     3361 tgatgatgac cgtataatcc gtccgcgccg aagcaatcga ggcgacgtgg atattgttgg
     3421 taatcaccct cagactgccg cgccgcctga ccagctccga tgccacggcc tccatcgtcg
     3481 tgccgatact gacaaacagc gacgaaccgt cggggatgtg ttccgcaatc agccgggcaa
     3541 tggcgttttt ttcgttttga caccgggttt ggcggtcggc ggacagaccc tccggcaagt
     3601 ttccgcccga agatgcgccg ccgtgatggc gtttcaggct gccgccctcc tccaactcgc
     3661 ggatgtcgcg gcgtatcgtc tgcggggtaa cgtccaatgc ggcggcaagc tcgtccaccg
     3721 acataaactg atgccggcgg acaaggctta aaatctctcc gtgcctttgg attttcggtt
     3781 tcatcgtttt ctgcctcctt gcatcgggat gccgatttta ccgcgttcaa cccaaagcgg
     3841 aaaacaccac catcagaaat ggggcggcga tattgaccac cacgccgaag ctgaccgcta
     3901 ccggcacgac ttccaaaccg cccgcaccct gaatcacggg caatgtgaaa tccatactgg
     3961 tcgcaccacc aacccccacc gccgcatccg gaaaacgctt catcagcagc gggataaatg
     4021 ccagtgcaaa cagctctcgt gccaaatcgt tcagcagcat gatgctgccc cataccgcgc
     4081 cgtaagcctc ggtcatgacc aaacccgaga gggaatacca accgaagccg gaagccatcg
     4141 ccaaaccttt cgtccacgac acaccgtctg tcgatgcggc aaacagcagc ccgcccgaaa
     4201 gagatgaaag cataaaccag accgacaacc gaatacccct gcggttgacc aaaacctgcc
     4261 gcaacgatac gccgctgctt ttgagctgta cgccgatgag gaacaccagc agcatcagac
     4321 aatacatgcc cgcgctttca gacggcatcc aaatatcgcg catcagtttg ccggatgcaa
     4381 atccgagcag cacgcatccg agctgcccca cactgcccga cacaccgacc gaaacgccct
     4441 tccctttccc ctttatccgc cacgggaata actttcccaa cactgccaaa gcaagcaggt
     4501 tcgccccgac cgtacaaaca aacagccaca gaaccgtcaa cgccatatcg tccaaccgcg
     4561 aacccaaatc ctccacgcgc gacaacgata cgccgatcag cagcagcacg gcatacacca
     4621 aaaccgatag caccttatcc aaagcgggca ggtaaggctt gggcacacgg ataaaaaatc
     4681 cggcaaacat cggtatcaaa accgaaatca atgtcatcag gctgtccatc tcctgacctc
     4741 ctttattgcc gcatgatatg tgcggtttaa aaattgccgt ctgaaaattg cagatacccg
     4801 catccatatt tcagacggca tcaggttcgc cattaaaaaa ccgcctgaag gttcaggcgg
     4861 cttatccgct ccggcattca atcttccaaa gtcttttcca aacgctccat acagttgccc
     4921 aaatggcggc gcaggatttt gaccacgcgg ttgcgcctgc ccgccagcag caggtcgagg
     4981 atttcgcggt gttctgaatg cgtatgcgta ttgatggcgt gtttttcctc gcgatgcacg
     5041 cccgccacgg cgacaatcag ggaagaccgc gcgcacagcg tgttcataat gtcgaacagc
     5101 acatcgttgc ccaccaggcg cgccagttcg acgtggaagg cattggacag gcggttccag
     5161 ccaacgcggt cgcccctgcc ggaggcctct tcttcgcgcc gtatcatcgc ataaagcggc
     5221 ttgaggcgcg tttccaaatc cggcaaatct gcgaggatat tcaaaatcat cgtctccatt
     5281 tcgatgcgcg cattgaacac atcctgcatt tctttcaaat cgggaacgtg gacgaacgcg
     5341 cccctgttgg gttgcaaatc gacaatcttg tcgtgcgcca aaagcgacag cgcgccgcgg
     5401 acggtgttgc gcgaacacac catctgacgg caaagttcgg attcggtcag ctttttgccg
     5461 ggcagcagca cctgatcggt aatgccgtcc aaaatcaggg cgtaaacacg gaacagctca
     5521 gaatcgtgcc gctcttcgag aatcagggaa gacgtagtcg gcgcatggat aatgtcgtcg
     5581 ttttcaaagt tcatgatgtt ttccgtattt ttacgctttc aaatttttta agatatttta
     5641 aggcggctgt gtttcaaatc gtgtcagagg aattaaagca ttgcacaaat ttattttata
     5701 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga tagtacggaa
     5761 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcca aggcgaggca
     5821 acgccgtact ggtttttgtt aatccactat aattcaataa attaatatat ggcttaaaat
     5881 aacgggatgc ccgcctcccg tcctcctgca gaagcgggcg ggtatcattt taaaacgcgg
     5941 catttaaaat ttgaccgaaa attgttgaca atccggaatc aagtctgcac aataccccga
     6001 caagtccaag tattataaag gctgaataaa gaggaaacag caggcagata tattcgggag
     6061 gtgcagtccg aatatatctg cttttttatg cgcctccgga ttgcctgccg cacctttccc
     6121 ttcagacggc atcagccgtt tccccataat gccgcccgat gcctatttat ctgccccggc
     6181 aatttcaaaa ctgtgggtaa tctttgccgc tttgcccaac ataatcgaag ccgaacagta
     6241 tttttcggca gacatctgaa cggcgcgctc aatggccgat tctttcaaat catgcccgaa
     6301 tactttgaaa tggatgtgga tttcggtaaa cacgcgcggc gcatcgtccg cccgtttcgc
     6361 cgtaaccgtc gcacggcagt cagtcacttt ctgacgctgt ttttcggcaa tcatcaccac
     6421 atcgatgctc gaacagcccg ccacgcccaa cagcagcatt tccaaagggc tgggcccgcg
     6481 cttagcctta ccttctgccg ccgacccctc cataacgacg ctgtgcccgc cttccgttgt
     6541 tccgacaaag cacattccgt ctatccattt tgatgtaacc tgcatggtgt cattcctgaa
     6601 aatagcgtta aaaccgcttt gcatatggcg ttattgtaaa caatttcaag cggcttatgc
     6661 agaaatatgg acaaaacggc aaaaaaacgc ctgaaaatcg atttacggtt tggcggactg
     6721 gccattgatc tgcaccgatt tgagtttcag cgtataggtt ttgccgtcgt cggtatagcc
     6781 gatttgtgcc ggaatattgt tcagggacgg tgcgaagaaa tacattaccg tatcgtcgcc
     6841 gcgccgcacc cgatatttga cgacttcggt ttccacgccg cctatgctgt attttcctgt
     6901 acccgcctta ttcaaaccgc cgacggaata aagttttttg ccgttggtga ttttcagccc
     6961 cggggggagt ttcgcgtcat ttgccgccaa ctgccaggca agcgtgaaca aatccatagc
     7021 cttggggctt tgctcggttt tgctctcgcc cgctttgccg taggttacgc tgccgtcggc
     7081 gaatttggct tccgcataca gtttgcccct gcgtatgtct ctatagtagg tagggtgcag
     7141 ggtattgccg acaaccgtac cgccggactc gaaacggata ttgtatagcg gcactttaat
     7201 cgtcgaaacg attttgtaag cattgccgct gcgttcaaat gtcatcgtgg cgggaatgcc
     7261 gtagctgccg gaatagtgca gcacggcgga ttggggcagc cctgccgcat acgcgcacgg
     7321 cagggcggcg gacaaaatgg cggcggaaaa tatattttta aaagtcttca tcatttgctc
     7381 ccgcccggtt tacgccgtca ggaaacgggc ggcatcggcg ttttccgaat ttctgacgcg
     7441 gtttccctcg ataatcaggc ggccggcggc aaaatcggca acggctttcg gatagagttt
     7501 atgctcgaca gccaaaaccc gggcggcaac atcgtctgcc gtatcgccgt cgagtatcgg
     7561 cacaacccct tgcgatacaa tcgggccgca atccagttcg gcagtaacga aatggatggt
     7621 gcagccggca acgcgacaac ctgcctccaa agcgcgttcg tgcgtatgaa gtccggtaaa
     7681 cgagggaagg atggacgggt gaatgttcat cagcctgcct tcgtaatggg cacagaactc
     7741 aggggtcaga atccgcataa aacctgccaa aaccaccaag tcgggttgat atgcgtcgat
     7801 tttttccatc atggcggtat cgaaggcaag tcgtgattca aagtttttat gattcaggct
     7861 gtcggtcgaa ataccctgac cggccgccca ttgcaaaccg gcagccgttt cgctgttgct
     7921 caacacggcg gcaatacgga cgttgggaat ggcggcattg acgattgcct gcatattgct
     7981 gccgcgtccg gaaatcagga tgacgatgtt tttcataatg gtgcgctttt gaaagggatg
     8041 ccatctgaac cgctgtttgg tggtttcaga cggcatttgc cgtaaaaatg cccgaaaacc
     8101 tgtttcgggc atggattcgg acttaattta cttttttgat gtcgacttga gccggctgct
     8161 tggcgggcgc gttttcgggt gcgccgattt tgaccagttt cacatcaaat accaaagtgg
     8221 cgttcgggcc gattttgtcg cccgcaccct gttcgcggta ggcaaggttg gacgggatgt
     8281 agaacgtggc ttcgccgcct tctttcagaa gctgtacgcc ttcggtccaa cccggaatca
     8341 cttggctcaa agggaaggtg accgggccgc cgttggcttt gctgctgtcg aataccgtac
     8401 catcaatcag gcggccttcg tattccacgg taacgatgtc gtctttggtc ggctgtttgc
     8461 cttcgccctg tttggtgatt ttgtattgca ggccggaagc agtggtcttc acgccgtctt
     8521 tggcggcatt ttctttcaaa aaggcttcgc ctttttcttt attggccttc gcgtccgcct
     8581 tgtgtttttc tacggcttta gcctgttgtt cctgaaggaa tttcatcatg acttcctgag
     8641 cctgctcttc agtcattttg atttctttgc cgtcatacac tgcctgcatg gcttcggtaa
     8701 agactttcaa atcgatttcc gcaccctgtt ccttcatttg cttcagggag cgtccgatgt
     8761 ccacgcccat cgcatagctt gcctgctgca tcgtgctgcc gatcgaagag gtgtcgccct
     8821 gcgcggcaga agcggcggca ggttcggatg cggcttcttt tttgccacag gcggaaagtg
     8881 ccaaagcggc ggaaagggtc agtgcgctga ttttgaaaat ggtgttcatg atggatcttc
     8941 gctgtcgata aggtcggaaa aacgggatta tagccgagtt tgaatgtttc aacacacagg
     9001 atgacacata aagcgtcaat cgtgtgttgc cctgttttgg aagggattga accttccaaa
     9061 ataagttttg attctaccgc cccgagggac agatgtccaa gtggcggggt tcaaccgata
     9121 aggaaatttt aatcaaatag aatcaagcct gtttaaattt tgtaaatgcg gcatttcaga
     9181 cggcatttta tgccttgccc tccatgccgt gatgttcgat ggcaaaaccg cttcggcggt
     9241 aggcggtaaa gcgttcgcgc gcgtcggcga gatcttccaa gctgttgccg acgatttcca
     9301 aaacgcgcgc gggcaagacc ggagcggtgt tccaaaaacc gtcggacagg ttcaaaacag
     9361 tcatgccttc gggaattcgg ggcggattgc cgccgcaagc aagcaggacg gatgtttcag
     9421 acggcatggc ttcttccatt tcccaaattt cgtgcggaat gaaactctcg gcttcgtatt
     9481 gccaaagcat tttgtccaat tcctgaagcc gcccgaacga atcggaccac accagtatcc
     9541 tgccgccgtc ccgaatcgca cgggcaatca ggcggcaggt gaaaatcgga acttgggcaa
     9601 cgtgcgtgta aaaggtggct ttcggcatat tgtttgaaca tttggcagga taatgccgtc
     9661 tgaaaggctt cagacggcat tgtggcaaaa ttaaagattc cgcagatagt tcagcagcaa
     9721 gggaacggga cggccggtcg cgcctttttc gctgccggat ttccacgccg tacccgcgat
     9781 gtcgaggtgc gcccacgggt agtcttcggt aaagtaagac aagaaggtgg cggcggtaat
     9841 cgtgcctgcg ccgggtgtgc cgatattggg gatgtcagcg aaattggatt tgagctggtc
     9901 tttgtaggtt tcaaagagcg gcagttgcca tgctttgtcg tccacgttgc gggaagcggc
     9961 aagcaggctg tcgaccaaat cttgattgtt gcccatcacg ccgctgacgt catgacccag
    10021 ggcgatgatg cacgcgccgg tcagggtggc gacgtcgatg acggctttgg gtttgaactg
    10081 ctcggcgtaa gtgagcgcgt cacacaaaat cagacggcct tcggcatcgg tgttcaacac
    10141 ctcgatggtc aagcctttca tgctttttac gacgtcgccc ggtttgttgg ccgcgccgga
    10201 aggcatgttt tcacaagtgg cgacgatggc aatcaagttg atcggcagtt gcagtttgac
    10261 ggcggcgcag aaggtactga tgacggttgc cgcgccgcac atgtcgaact tcatttcgtc
    10321 catgttcaga ccgggtttga gggaaatgcc gccggtgtcg aaggtgatgc ctttgccgac
    10381 caataccaca ggcgcggctt ctttgtcggc tgcgccaaaa tagctcagtt cgaccaaata
    10441 gggatcttcg acgctgcctt tggcgaccga ccaaaacgaa cccatgtttt ctttgatgta
    10501 gtctttttcg atgattttgg cgtgcgcgcc cagtttttcg gcttcggctt tggcggtgcg
    10561 cgctaaaaat tcgggcgtgc attcgttcgg cgcagcattg cccaagtcgc ggcagaggct
    10621 ttgtccgtaa acctgtgctt cggcgacgcg caaggcttct ttgacggcgg cttcgtgcgc
    10681 ggtatggaac acggcagttt caaatttggc gggcttggct tcttttttgt agcggtcgaa
    10741 acggtaggcg gcgttgccga acgcaatcgc aaacgcttcg gctacggcgg cggcttgcgc
    10801 ttcatcgaag gcatggatgt ccacattgac cgtttcctga ttttgcgccc atttggcggc
    10861 ttcggcggcg gccttgttca atgcggcgcg gccggtgctt ttcagacagc atacggcaac
    10921 agcctgcaaa ccgttgcctg tcgggatttt tgtgtcggca aaattttgac cttcttcaag
    10981 cgaagacaaa agggcaaggg cggtcggatg gtttaattgc gcctcttcgg tgcagacaaa
    11041 taactgtgcg cctgcctgct gttcctgcaa gatttcggtt tttgtgctaa attccacgtt
    11101 tattctcctg attgagacgg ttgtcggtag ttttcggacg gcctttcgct caaaagaccg
    11161 tctgaagacg gctggcacga ttgtacccca tttgaagcac cgtctgaaac cttgcgcgga
    11221 caatccgcct gcgccgaacc gcttaccgcc ccctgaccgc gattctatga tttatcaaag
    11281 aaacctcatc aaagaactct cttttaccgc cgtcggcatt ttcgtcgtcc tcttggcggt
    11341 attggtctcc acgcaggcaa tcaacctgct cggccgtgcc gccgacgggc gtgtcgccat
    11401 cgatgccgtg ttggcattgg tcggcttctg ggtcatcggt atgacgccgc ttttgctggt
    11461 gttgaccgca tttatcagta cattgaccgt gttgacccgc tactggcgcg acagcgaaat
    11521 gtcggtctgg ctatcctgcg gattggcatt gaaacaatgg atacgcccgg tgatgcagtt
    11581 tgccgtgccg tttgccgttt tggttgccgt catgcagctt tgggtgatac cgtgggcaga
    11641 gctacgcagc cgcgaatatg ccgaaatatt gaagcagaag caggaattgt ctttggtgga
    11701 agcaggcagg ttcaacactt tgggcaagcg caacggcagg gtttattttg tcgaaacctt
    11761 cgacaccgaa tccggcatca tgaaaaacct gttcctgcgc gaacaggaca aaaacggcaa
    11821 cgacaacatt atcttcgcca aagaaggcaa tttctcgctg aacgacaaca aacgcacgct
    11881 tgaattacgc gacggctacc gttacagcgg cacacccggc aaagccgact acaaccgcgt
    11941 ttctttccgg catctcagcc tgattatcag caccacgccc aaactcatcg accccgtttc
    12001 acaccgccgt accattccga ccgcccaact gatcggcagc agcaatccgc aacatcaggc
    12061 ggaattgatg tggcgcatct cgctgaccgt cagcgttttg ctgctgtgcc tgctcgccgt
    12121 gccgctttcc tatttcaacc cgcgcggcgg acacacctac aatatcttga ttgccatcgg
    12181 tttgtttcta atttaccaaa acgggctgac cctgcttttt gaagccgttg aaaatggaaa
    12241 aatccatttc tggctcggac ttctgcctat gcacatcatc atgtttgccg ttgcactcat
    12301 tctgttgcgc gtccgcagta tgcccgcgcg gccattttgg cgggcggttg ccgaaagcct
    12361 gacaccgaaa ggcagaaaat gaacctgatt tcacgctaca tcatccgcca aatggcggcg
    12421 atgaccgttt acaccctcct cgccttcctc gctttgtaca gcttttttga aatcctgtac
    12481 gaaaccggca acctcggcaa aggcagttac ggcatatggg aaatgctggg ttacaccgcc
    12541 ctcaaaatgc ccgcccgcgc ctacgaactg atgcctctcg ccaccctgat aggcggactg
    12601 ctttccctcg gcaggctggc tgccgacagc gaactgaccg tcatcaaagc cagcggcatg
    12661 agcaccaaaa agctgctgtt gattctgtcg cagttcggtt ttatttttgc tattgccacc
    12721 gtcgcgctcg gcgaatgggt tgcgcccaca ctgagccaaa aagccgaaaa catcaaagcc
    12781 gcggccatca acggcaaaat cagcaccggc aataccggcc tttggctgaa agaaaaaaac
    12841 agcattatca atgtgcgcga aatgttgccc gaccataccc tgctgggcat taaaatctgg
    12901 gcccgcaacg ataaaaacga actggcagag gcagtggaag ccgattccgc cgttttgaac
    12961 agcgacggca gttggcagtt gaaaaacatc cgccgcagca cgcttggcga agacaaagtc
    13021 gaggtctcta ttgcggctga agaaaactgg ccgatttccg tcaaacgcaa cctgatggac
    13081 gtattgctcg tcaaacccga ccaaatgtcc gtcggcgaac tgaccaccta catccgccac
    13141 ctccaaaaca acagccaaaa cacccgaatc tacgccatcg catggtggcg caaattggtt
    13201 taccccgccg cagcctgggt gatggcgctc gtcgcctttg cctttacccc gcaaaccacc
    13261 cgccacggca atatgggctt aaaactcttc ggcggcatct gtctcggatt gctgttccac
    13321 cttgccggac ggctcttcgg gtttaccagc caactctacg gcatcccgcc cttcctcgcc
    13381 ggcgcactac ctaccatagc cttcgccttg ctcgccgttt ggctgatacg caaacaggaa
    13441 aaacgctaaa ccaatgccgt ctgaccccct tcggacggca ttggttttta ttatcatatt
    13501 cctgtagaca gatagccgtt ccctattata gtggattaac aaaaaccagt acggcgttgc
    13561 ctcgccttgc cgtactattg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
    13621 atccactata cattacctgt cataacagtt ccatttttgt taaaactagt ctatgatagc
    13681 ggtacaaata ttgtttacaa tatttaacgc aaatcatttg caacccgaca aaagaaaaac
    13741 agaaaaagga acaaagagat gttagaagcc tatcgtaaag ccgccgccga gcgcgccgcc
    13801 ctcggcattc ccgccctccc tttgaacgcg cagcaaaccg ccgatttggt tgagctgctg
    13861 aaaagcccgc ccgcaggcga aggcgagttc ttggtcgaac tgcttgccca ccgtgttccg
    13921 cccggtgtgg acgatgccgc caaagtcaaa gcctcattcc tggctgccgt tgccgaaggc
    13981 agcgcgtcca gcccgctgat ctcccccgaa tatgcgaccg aactcttagg tacgatgctc
    14041 ggcggttaca atatccacgc cttaatcgaa ctcttggacg acgacaaact cgcgcccatt
    14101 gccgccaaag gcttgaaaca tacgcttctg atgttcgatt ccttccacga cgttcaggaa
    14161 aaagccgaaa aaggcaacaa atacgcgcaa gaagttttgc aatcttgggc agatgccgaa
    14221 tggttcgcct cacgcgccaa agttcccgaa aaaatcaccg ttaccgtttt caaagttgac
    14281 ggcgaaacca atacagacga cctctccccc gcgcccgacg cgtggagtcg tcccgatatt
    14341 ccgctgcacg cgctggccat gctgaaaaac ccgcgcgacg gcatcacgcc cgacaaaccg
    14401 ggcgaagtcg gtccgattaa attgttggaa gaactcaaag ccaaaggcca tccggttgct
    14461 tacgtcggcg acgtggtcgg tactggttct tcacgcaaat ccgcgaccaa ctccgtcatt
    14521 tggcataccg gcgaagacat tccgttcgtg ccgaacaaac gcttcggcgg cgtgtgtttg
    14581 ggcggcaaaa tcgcgccgat tttcttcaat acccaagaag attccggcgc gctgccgatt
    14641 gaagtcgatg tatctgctct aaaaatgggc gatgtcgtcg atatcctgcc ttatgaaggc
    14701 aaaatcgtga aaaacggcga gactgttgcc gagtttgaat tgaaatcaca agtattgctg
    14761 gacgaagtgc aagccggcgg ccgtatcaac ctgattatcg gccgaggtct gaccgccaaa
    14821 gcgcgcgaag ccctgaaact gcctgcctct actgcattcc gcctgccgca agcgcctgcc
    14881 gaaagcaaag ccggtttcac cttggcgcaa aaaatggtcg gccgcgcctg cggtctgccc
    14941 gaaggacaag gcgtgcgccc gggtacttac tgcgaaccgc gtatgacgac ggtcggctcg
    15001 caagacacga ccggcccgat gacccgcgac gagttgaaag acttggcttg tttgggcttc
    15061 tccgccgata tggtgatgca gtctttctgc cacaccgccg cctatccgaa acctgtcgat
    15121 gtaaaaaccc ataaagaact gcccgccttt atttccaccc gtggcggcgt gtccctgcgt
    15181 ccgggcgacg gcgtcatcca ctcgtggctc aaccgcctgc tgctgcccga taccgtcggc
    15241 accggcggcg acagccatac ccgtttcccc atcggtattt ccttccccgc cggctccggc
    15301 ttggttgcct ttgccgccgc aacgggcgta atgccgctcg atatgcccga gtctgtattg
    15361 gtacgcttca gcggcaagct gcaaccgggc gtaaccctgc gcgatttggt gaacgccatc
    15421 ccgctgtacg caatcaaaca aggtttgctg accgttgcca aagccggtaa gaaaaacatc
    15481 ttctccggcc gcatcctcga aatcgaaggc ctgcctgatt tgaaagtgga acaagccttt
    15541 gaattgaccg acgcatccgc cgaacgctcc gccgccggct gtaccgtgaa gctcaataaa
    15601 gagccgatta tcgagtacat gaaatccaac gtcgtgttga tgaaaaacat gattgccaac
    15661 ggctatcaag atccgcgcac tttggaacgc cgcatcaaag ctatggaaaa atggctggca
    15721 aatcccgagt tgctcgaagc ggataaagat gccgaatacg ccgccgtgat tgaaatcaac
    15781 atggacgaca tcaaagagcc gattatcgcc tgcccgaacg atccggacga cgtatgcttc
    15841 atgtccgaac gttccggcac caaaatcgac gaagtgttca tcggttcgtg catgaccaac
    15901 atcggccact tccgcgctgc ttccaaactt ttggaaggca aggcagacac ccccgtccgc
    15961 ctgtggattg cgccgccgac caaaatggac gcgaaacaat tgtccgacga aggacactac
    16021 ggcgtactcg gacgtgccgg cgcgcgtatg gaaatgccgg gttgctcctt atgtatgggt
    16081 aatcaggcgc aagtacgcga aggtgcgacc gttatgtcca cctccacccg caacttcccg
    16141 aaccgtttgg gtaaaaacac ctttgtttac ctcggttcgg cggaattggc agcgatttgc
    16201 tccaaactgg gtaaaatccc gaccgttgaa gaatatcaag ccaatatcgg catcatcaac
    16261 gaacagggcg ataaaatcta ccgctatatg aacttcaacg aaatcgacag ctacaacgaa
    16321 gtagccgaga ctgtgaacgt ttaatccccg tcatccgtat gaagtaaggg attgaccgca
    16381 atgccgtctg aacaaccttc agacggcatt gcaacattcc gctaaccctt ctttccgcaa
    16441 acgctgcaaa tacggcgttc acgcccccac ataaaggaaa cgacagtgaa cctgaaaaac
    16501 cgccattttc tgaaactttt agacttcacg ccggaagaaa tcaccgccta cctcgacctt
    16561 gccgccgaat tgaaagccgc caaaaaagca gggcgcgaga ttcagcggat gaaagggaaa
    16621 aacatcgccc tgatttttga aaaaacctct actcggacgc gctgcgcgtt tgaagtcgcc
    16681 gcgcgcgatc aaggcgcggg agtgacttat ttagagccgt ccgccagcca aatcgggcat
    16741 aaggaaagca tcaaagacac cgcccgcgtg ttgggcagga tgtacgatgc catcgaatat
    16801 cgcggtttcg gtcaggaagt tgttgaagaa ttggcgaaat acgcgggcgt acccgtgttc
    16861 aacgggctga ccaacgagtt ccatcccaca caaatgcttg ccgacgcact gactatgcgc
    16921 gaacacagcg gcaaaccttt gaaccaaacc gcgtttgcct acgtcggcga cgcgcgttac
    16981 aacatgggca attccctgct gattttaggg gcaaaattgg ggatggacgt gcgtatcggc
    17041 gcaccgcaaa gcctgtggcc gtctgaaggc attattgccg ccgcacacgc cgccgccaaa
    17101 gaaaccggcg caaaaattac cctgaccgaa aacgcgcatg aagccgtgaa gaatgttgat
    17161 tttattcata ccgatgtgtg ggtcagcatg ggcgagccga aagaagtctg gcaggaacgc
    17221 atcgatttgc tgaaagatta ccgcgttacg cccgaactga tggcggcatc gggcaatccg
    17281 caagtcaaat tcatgcactg cctgcccgcc ttccacaacc gcgaaaccaa agtcggcgaa
    17341 tggatttacg aaaccttcgg gctgaacggt gtggaagtta cagaagaagt attcgaaagc
    17401 cccgccagca tcgtgttcga tcaggcggaa aaccgtatgc acacgattaa agcggtaatg
    17461 gtcgcggctt tgggcgactg acagaactgt gcctgtttaa attcatccgc aacacagata
    17521 ccgtctgaac acgatgttca gacggtatcc atatatagtg gattaaattt aaaccagtac
    17581 ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
    17641 tttttgttaa tccactataa aaaaactgcc tacaccatgt gtaggcagtc ccgtttgaaa
    17701 acaatcagtt tttgtcttgg tcaaccaatt tgttggcagt aatccaaggc atcatggcac
    17761 gcagttgtgc gccgactttt tcaacttggt ggtcggcatt cagacggcgg cgggcagtca
    17821 tagacgcgta gttgacatta ccctcttgga taaacatttt tgcgtattcg ccggtttgaa
    17881 tgcgtttcag ggcattgcgc atggcttctt tgctggaagc attgaccact tcagggccgg
    17941 taacgtattc gccgtactcc gcattgttgg aaatggagta gttcatattg gcaataccgc
    18001 cttcgaaaat caggtcaacg atcagtttca tttcgtgcag acattcgaag taagccattt
    18061 caggcgcgta accggcttcg gtcagggttt caaaacccgc cttgatcaac tcgaccacgc
    18121 cgccgcacaa tacggcttgt tcgccgaaca gatcggtttc ggtttcttcg cggaaagtgg
    18181 tttcaatcac accgcctttg gtgccgccgt tggcagccgc ataagacagg gcgatgtctt
    18241 tggctttgcc ggaattgtct tggtaaacag caatcagaga aggcacgccg ccgccgcgtt
    18301 tgtattcact gcgtacggta tgacccgggc ctttgggggc aaccataatc acgtccaagt
    18361 cggcacgcgg aacgatttgg ttgtagtgca cgttgaagcc gtgtgcaaat gccagcgttg
    18421 cgccttcttt cagattggct gcaacttcgg cgtgatagac ggcaggcatg gtttcgtcag
    18481 gcagcagcag cataacgaca tcggcttctt tggtcgcttc agcaacggtt ttgacgacat
    18541 ggccggctgc ttcggctttt ttccaagaag aaccttggcg cagaccgatc accacgttta
    18601 cacccgaatc tttcaggttg gcagcatggg catgaccttg cgaaccgtaa ccgatgatgg
    18661 caacggtttt gcctttgatc agggacagat cggcatcttt atcgtaatag acttgcattt
    18721 gatttccttt aaggtaaatg gttgtcgaag ccttaaaatg ttgagcggct tcggacgggt
    18781 taaacagagt gtgccgctta agcggcaact tcattcatca atacgatttc caacgcttcg
    18841 gttttgccgt cgacggactg gacgaaggct tggaaatgcg cgctggcgtt atgttcgtca
    18901 atagctgctt gagatttcca attttccacg aaaacaaaac ggttcggttt gccgatttcc
    18961 tgatggagat cgtagctgat gttgccctct tctgcacggc tggctttgac cagttcttta
    19021 aactgtgcta ccagtgtttc cgtgtattcc ggtttgacgg taaccagtgc gacaatttta
    19081 atgttcgaca taaatctctc ctgccgttcg tttttcagac ggcattcaaa taccgtgccg
    19141 tctgaaaggt tacggcgtta aattttcaaa atacgctcac cgcgaccgat gccggccgcg
    19201 cctgtgcgta cggtttccaa aatttgggcg cgtccgaccg tttccaaaaa ggaatccagc
    19261 ttgtcggtcg agccggtaat ttcaatcgta tagctgcggt cggttacgtc gatgatgctg
    19321 ccccggtaga tttcggtcaa gcgtaaaaat tcgtcgcggt ctttgccggc ggcacggact
    19381 tttaccaaca tcagttcgcg ttcgacaaaa cggctttcat tcaaatcgac cactttaatc
    19441 acttcaatca atttattgag ttgcttggtg atttgttcga tgacctgctc gtctccgtgg
    19501 gtaacgatgg tcatacggga cagggttttg tcttcggtcg gcgcaaccgc caaagaatcg
    19561 atattgtaat cgcgtgcaga gaacaaaccg accacgcggc tcatcgcacc tgattcgttt
    19621 tcaatcagaa cagataagat atgtcgcatt tgcctctcct tacgcctttt cgtccgcacg
    19681 catatgcggc ggaagtacca tttcgtccaa acctttgccg ttgccgacca tgggcatcac
    19741 attctgtttc tggtcggtca ggaagtcgat aaacaccagc ctgtcttttt ggttcaatgc
    19801 ttccaacaac gcaccttcca catcagactt cctgtccacg cggataccga tatggccgta
    19861 tgcctcggca agtttgacga aatcgggcaa agaatcgaaa taggtttccg actctcgacc
    19921 gccgtaatat atttcctgcc actggcgtac cataccaaga taaccgttgt tcagcgtaat
    19981 gacgttaacc ggaatccgat attggaaaca ggtggacagc tcttggatgt tcatctgaat
    20041 cgagccgtcg ccggtaatac agaatacgtc ttgatccggg gcggcaagtt ttgcaccaat
    20101 cgcataaggc agacccacgc ccatcgtacc caaaccgccg gaattgagcc attggcgcgg
    20161 acgttcgaag ggataatatt gagccgcaaa catttgatgc tgccctacat ccgatgtgat
    20221 gattgccgaa ttgccggtaa tctcggcaag cttctgaatc acatattgtg gcttgataat
    20281 ttcgctgccg ttgtcaaacc acaagcaatc gcgggaacgc cattcctcta tggttttcca
    20341 ccatttgccc aaagcatctt cagacggcac ggactcttgt ttttgccaca gcgcaaccat
    20401 ctcggacaaa acgtttttca cgtcgccgac aatcggaatg tccgccttca cgcgtttggc
    20461 gatgctggaa ggatcgacat cgatatggat aaccttcttc gccttctcga aaaatttgga
    20521 cggtacggaa accacacggt cgtcaaaacg cgcacctacg gcaagaacga catccgcatt
    20581 ctgcatggca aggtttgcct cgtaagtacc gtgcataccg agcataccga ggaattggcg
    20641 gtcgccggaa ggataagcgc ccaagcccat cagcgtaccc gtgcacggag cacccgtcat
    20701 tcggacaaat cgggtcagct cttcagaagc attacccaac accacgccgc cgccaaaata
    20761 gacgaccgga cgtttggcag atgccaacat ctgcacggcc tttttaatct gaccgatatg
    20821 cccttgaaca accggttgat acgaacggat aaaaatgtct tcctgaggat agctgaattt
    20881 cgccatcgcc tgcgtaacat ctttcgggac atcaaccacc acaggccccg gtcggccgct
    20941 tgcggcaatt tggaacgcct ttttaatggt ttccgccaac tcattgatat ccgtaaccag
    21001 gaaattgtgt ttgacgcacg gacgggtaat acccaccgta tcaacttctt ggaacgcatc
    21061 cgtaccaatc agggaattgc ctacctgccc gctgatgacc accatcggaa tcgaatccgt
    21121 ataggcagta gcaataccgg tcagtgcatt ggtaacgccc gggccggatg taaccaatgc
    21181 cacgcccacc ttaccgctga cgcgcgcata cgcatctgcc gcgtgtactg ccgcctgctc
    21241 gtgacgggtc agaatgtgct tgaatttatt gagttggaaa agggcatcgt agatttcgat
    21301 aaccgcaccg ccaggataac cgaaaacgta ctcgacacct tcggctttga gactctgcac
    21361 tatgatttgc gcgcctgata actgcataac gacctctttt atacggtttc aaaccaatag
    21421 ggacaaaccg ctttgccaca gcacctgtaa tgcaattcca ccaagcagcg atttagggta
    21481 cgcgcattgg gggaacacgg caacagacgg attatccaat caattggaaa ggaacacaga
    21541 gtttgtgaaa aagagtagaa acgataacgc aaaccgacag ttcaatcaag aaaaatcttt
    21601 catcttttaa tattttttga aagcagagaa attattgatt gattttaaaa gaataaaatc
    21661 aggagtacct tttttgaaag atggaaattg ttgacagttt atgcaggagg ggcagatgtg
    21721 aaaaaccctt cttcgatatc aagaattgta aaatttacat ggtttcatcc caataaagac
    21781 tcgggatatt gattgaactt gattttattt ttgatatatc aaaaatattc ccaaccatac
    21841 ttcctgaaaa tggctcattg caccggactg tattggacgg cattaacaga accaagaggg
    21901 ctaacaacga cttaatatat tgattgtata gtggattaac aaaaatcagg acaaggcgac
    21961 gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact ggtttaaatt
    22021 taatccact
//
LOCUS       DAWFJW010000022        20940 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFJW010000022 DAWFJW010000000
VERSION     DAWFJW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.022"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(92..783)
                     /locus_tag="WGR19_000692"
                     /pseudo
     CDS             complement(92..783)
                     /locus_tag="WGR19_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1089..1646)
                     /locus_tag="WGR19_000693"
     CDS             complement(1089..1646)
                     /locus_tag="WGR19_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798261.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1890..2048)
                     /locus_tag="WGR19_000694"
     CDS             complement(1890..2048)
                     /locus_tag="WGR19_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798262.1"
                     /translation="MKPPLPICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2045..2887)
                     /locus_tag="WGR19_000695"
     CDS             complement(2045..2887)
                     /locus_tag="WGR19_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0798263.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3125..4045
                     /gene="pyrB"
                     /locus_tag="WGR19_000696"
     CDS             3125..4045
                     /gene="pyrB"
                     /locus_tag="WGR19_000696"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0798264.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4055..4513
                     /locus_tag="WGR19_000697"
     CDS             4055..4513
                     /locus_tag="WGR19_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0798265.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4578..5243
                     /locus_tag="WGR19_000698"
     CDS             4578..5243
                     /locus_tag="WGR19_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798266.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5306..6523)
                     /locus_tag="WGR19_000699"
     CDS             complement(5306..6523)
                     /locus_tag="WGR19_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0798267.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6665..7210
                     /gene="def"
                     /locus_tag="WGR19_000700"
     CDS             6665..7210
                     /gene="def"
                     /locus_tag="WGR19_000700"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0798268.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7297..8223
                     /locus_tag="WGR19_000701"
     CDS             7297..8223
                     /locus_tag="WGR19_000701"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0798269.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDAGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8302..9561
                     /gene="rsmB"
                     /locus_tag="WGR19_000702"
     CDS             8302..9561
                     /gene="rsmB"
                     /locus_tag="WGR19_000702"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0798270.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLVHIRAAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9527..10126
                     /locus_tag="WGR19_000703"
     CDS             9527..10126
                     /locus_tag="WGR19_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0798271.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10126..12246
                     /locus_tag="WGR19_000704"
     CDS             10126..12246
                     /locus_tag="WGR19_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0798272.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEVGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12239..13516
                     /locus_tag="WGR19_000705"
     CDS             12239..13516
                     /locus_tag="WGR19_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0798273.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNREVGAAVKRASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13607..14794
                     /gene="dprA"
                     /locus_tag="WGR19_000706"
     CDS             13607..14794
                     /gene="dprA"
                     /locus_tag="WGR19_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0798274.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGEKGIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGSRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14820..15281
                     /locus_tag="WGR19_000707"
     CDS             14820..15281
                     /locus_tag="WGR19_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0798275.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15353..17659
                     /gene="topA"
                     /locus_tag="WGR19_000708"
     CDS             15353..17659
                     /gene="topA"
                     /locus_tag="WGR19_000708"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0798276.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNSKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17732..18427)
                     /locus_tag="WGR19_000709"
     CDS             complement(17732..18427)
                     /locus_tag="WGR19_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798277.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            19117..19494
                     /locus_tag="WGR19_000710"
     CDS             19117..19494
                     /locus_tag="WGR19_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0798278.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19583..20152
                     /gene="rsmD"
                     /locus_tag="WGR19_000711"
     CDS             19583..20152
                     /gene="rsmD"
                     /locus_tag="WGR19_000711"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0798279.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGRL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20275..20526
                     /locus_tag="WGR19_000712"
     CDS             20275..20526
                     /locus_tag="WGR19_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0798280.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20623..20706
                     /locus_tag="WGR19_000713"
     tRNA            20623..20706
                     /locus_tag="WGR19_000713"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20657..20659,aa:Tyr,seq:gta)
     gene            20737..20810
                     /locus_tag="WGR19_000714"
     tRNA            20737..20810
                     /locus_tag="WGR19_000714"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20769..20771,aa:Gly,seq:tcc)
     gene            20820..20894
                     /locus_tag="WGR19_000715"
     tRNA            20820..20894
                     /locus_tag="WGR19_000715"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20852..20854,aa:Thr,seq:ggt)
BASE COUNT         5236 a         5309 c         5713 g         4682 t
ORIGIN      
        1 aagtgaatgg gtttcgtact atttgtactg tctgcggctt cgtcgccttg tcctgattac
       61 tatataaaat aaatgggcaa aaatcggttt attatcgttt ttgccgcatt tggatttgtt
      121 ctaccgtaaa acgtgtttga cgaacgggat tcttattaaa aaacatctga tttctaacaa
      181 aatcagtatt ttttggcacg atggctaaaa tttttccttc catttcgcca tcacgtgttt
      241 tccatgcgct caagaattgt gatttgctca ttgagacgtg ccccagcgat ggatcagcca
      301 gcaaaacagt ttctccgtta ataccgttca ataccgaaaa atggttgttt ttacggtatt
      361 ttaaatacac aattacagga atttttagtt gtaccaactg ttcaaatggc aaagcataac
      421 cttgtgcttc aaaacccagt tcgggcatta tgcgttgcat atcatcaaaa gaagcacgca
      481 tttgggtttt atccattttg tccaagattt ccgcttcaga ataatgtctg ccataaaaat
      541 tattcagtaa cgtggcaatc gaagccgcgc cgcaagaaaa atccaaatct tgttttacta
      601 tgccggaatc tcgccgtgct ttccaacttc gtacatggat gttttggtaa gaagcggggg
      661 gtcaacaaac ataggccaag caaaaactat atttggggcg aaaccaatca aagccgcata
      721 atttatcaat ttataaagat tttttatcat aatatgtata cgcggaataa acgcatgaaa
      781 cataaaaaaa taaaatcata tgcaatttta ttgcatgaat aaatatgaat aaaatagata
      841 atgatgggag taaatacgcc atgtattttg gaagtttaaa tttattaata ataaaataat
      901 ttatcgtagc gcaaataaat cccaaaattg gaacgattag aaaaaaaatt aatacaccca
      961 tcatcatgca aactctatat taataaatag ctaactaatt ctattgtgga aattagttag
     1021 ctaactaaaa gttattaatg attatttccg agaattgact gcattgttgg cagcattggc
     1081 cccaaaacct agtgcatgaa tacccggtct ccatgccaaa ttcccagcca atccgcctcc
     1141 ggcagcagca gccagccctg ttttgccgct actcctgttg ccgcaccgat tcctgccgca
     1201 gtagccgcgc cttgcgcagt tcctaattta ccatgattat acaaattagc accatgatac
     1261 tcccatgcac ctaatgcacc gccaaaagca gcggctgcaa taatgggaac aaattcacct
     1321 tgtgtttctt tcatttcagc ctgtgataat tgaattgctt tcacattttg gctgtcaaaa
     1381 acttggctgt ctaaattttg cgccattaca ggtgtaatca tcatagccat tacagttgca
     1441 attttcgttg cgctggtttg cacataaata ggattagcaa attcgctttg attgcgttca
     1501 gtgttgatgt agctaatact gctttctagt ttgaatttac ccttgtcagt caataattct
     1561 tccaaactta aaggtaagtc agcataagca atttggctgg caacagtcaa aataaaatct
     1621 attagacatt tgtgtttttg catcatttcg tttgattttc tagtttttga gaatgataca
     1681 aagtttttta caaagtaaag agtcactctg aaaaacattt tttcattata aatcaaaata
     1741 ttgatagaat aaatagcgag catcgattca cggtgtactt tagtgcaaag cgtacaacgc
     1801 gagcctgaac caccagccgc aacaggaaaa gaaagccgat agagtgcaat gcttgccaac
     1861 gtgcaagcga gcttgcaaga acgcttggct caacgagagc aggcaagaca gaaagcagaa
     1921 agcaggatag gagcggtaac gcaaaggtct cgggctttga tttcgccgta aaccctgctg
     1981 ccgccttgtc cggaaagggc gcaggcggcg agtgccgaca gggtgcagat ggggaggggg
     2041 ggtttcattt ggggtcgcaa cggaagtggt atgcgcagat ttcaaaaccg tttttgaaat
     2101 acaggcggtg cgcgtcggca cggtcgtggt tgacgtggac gttgaggtgg attttggtta
     2161 cccctgtttc cgcgccgatt ttgcggactt cttccaaaag gcgcgaggcg tagcctttgc
     2221 ggcggctttg cggcagggta acgatgtcat cgatgtggat gtggcggccg ctggcgaggg
     2281 tgcaggcttc gcggaagccg cagacggcga cggcattgtg tttgccttct tcaaaaatac
     2341 ccagcaggcg gtagccttgg gggcgttgga ctttgttgat ctgttcggta aagcggttga
     2401 tgtcggtcag ggcggaacgc aaaacgctca aggctgcaaa ggcggtggcg gtgtcgtccg
     2461 cgccgatttc gcgcaaaacg taggatgcgc ccgaggcggt ctgttcctgt gctttctcgg
     2521 cggcgtgttt ttcttcgatt gcctgtgcca gcatgacgtg ttcgtcggca gggttgtttt
     2581 gtccgccctg ttcgcgttct tcgagcaggg ctttgcagtc gatgacgcgc aggtcgttgt
     2641 cggcggcaaa gtccatcagg aagcggaaca tttgggggtt gtcggtttcc agttttttat
     2701 cgacggcgac gcagcggatg ttgtccacca gcatggggcg cacccatttc gaataggaaa
     2761 gcctgtggtc tttggtgaac gaggacagaa tgccgcacag gcgttccgcc cagtcgctgg
     2821 gacggaaaat cttgccggaa ctcgttgtgc cgtggatgac gacttcgtag gggttgcaga
     2881 ctaacatggc ggcttcctga aaagaaatgt ctggcgcgat tataccttat gcttatgcgg
     2941 gcgtgtttgg atatgccgtc tgaaaagtac gggattcgtg cggtaaaact ttgcggcggc
     3001 aaatgtgcga taatacgcgc cgtattgccg cttttgcgaa gctgttccgc aaacatacgg
     3061 gcggcgtgga cgacgtataa ccggataccc gcctgacgcg ggttttttac ggaagggggc
     3121 aaaaatgcct aatccgcttt acagacagca catcatctcg atttcggatt tgtcgcgcga
     3181 acagttggaa tgcctgcttc agacggcatt gaagctgaag gcgcatccgc gcggcgattt
     3241 gttggaaggc aaacttatcg gttcgtgctt ctttgaaccg tccacgcgca cgaggctgtc
     3301 gtttgaaacg gcggtgcagc ggctgggcgg caaggtcatc ggcttttcgg acggcgcgaa
     3361 taccagtgcc aaaaaaggcg agacgcttgc cgataccgcc cgcatcattt ccggctacac
     3421 cgacgcaatc atccaacgcc accccaaaga cggcgcggcg cgcgtggcag cggagttttc
     3481 gcgcgtcccc gtcatcaacg ccggcgacgg caccaaccag caccccagcc agaccttgtt
     3541 ggatctggta acgatttatg aaacgcaggg gcgtttggac aagctcaaaa tcgccatggc
     3601 gggcgacttg aaatacgggc gcaccgtgca ttcgctttgt caggcgttga aacgctgggg
     3661 ctgcgaattt gcctttgttt cgccgcccag cctcgcgatg cccgactaca ttaccgaaga
     3721 gttggacgaa gccggctgcc gataccgtat cctcggcagt ttggaagagg cggcggaatg
     3781 ggcggacatc ctgtatatga cccgcgtcca gcgcgaacgt ttcgatgaac aggaatttgc
     3841 caaaatccaa ggcaaattca acctcgaagc gtctatgctc gcccacgcca aaccgaacct
     3901 gcgcgtgctg caccctttgc cgcgcgtgga cgaaatccat cccgatgtcg atgccacgcc
     3961 gcatgcctat tatttcgagc aggcaaccaa cggcgtttat gcgcgtatgg cgatattgtc
     4021 gctggtgttg aacgaagaag tgtgaggaac cgatatggaa accccgaaac tcagtgtcga
     4081 agccattgaa aaaggtacgg ttatcgacca tattcccgcc ggcagggggc tgaccatcct
     4141 gcgccagttc aaacttttgc actacggcaa cgcagtaacc gtgggcttca acctgcccag
     4201 caaaacccaa ggcagcaaag acatcatcaa aatcaaaggc gtgtgcttgg acgacaaagc
     4261 cgccgaccgc cttgccctgt ttgcacccga agcagtggtc aacaccatcg acaatttcaa
     4321 ggttgtgcag aaacggcatt tgaacctgcc cgacgaaatc gccgaagtgt tccgctgtcc
     4381 gaacacgaat tgcgccggcc acggcgagcc ggtcaaaagc cggttttatg ttaaaaaaca
     4441 caacgggcag acgcggctga aatgccatta ctgcgaaaaa acctacagcc gggattcggt
     4501 ggcggaagcc tgacggattc ccttaaaccg agtgggcggc atttcgtctg ccgcctgttt
     4561 tgccaatctg aaatggaatg atgatgcacg cttctgtcca aagccgtttc gcaccgatac
     4621 tttatgtttt gattttcttt gccggttttt tgaccgcgca aatctggttc aatcagaaag
     4681 cctatactga agagctgcct ccgcttctgt ccgcattgtc cgctgtcgcg ctggtgtggc
     4741 tggcgtgggc gttcgtgtcg gcgcgttcaa aggctaaggc ggaaaagttc taccgcgaaa
     4801 aaatgataca gaacgaaagc atacaccccg tcctgcacgc ttctttgcaa cacttggaac
     4861 acaagccgca aatgctcgcc ctgctggtca aaaaccacgg caaagggatg gcggaacagg
     4921 tcaggttcaa ggcggaagtg ctgcccgacg acgaagacgc gcgcacgatt gccgccgagt
     4981 tggcaaaaat ggatatgttt gcattgggga cggacgcggt cgcctcgggc gaaacctatg
     5041 gacgcgtgtt cgccgatatt ttcgagttgt cggcggcttt ggaagggcgc gcgttcaaag
     5101 gaatgttgaa actgacggcg gaatataaaa acatcttcgg cgatgcctgc cgttcggaaa
     5161 cggcgttgga gttgggcgcg ctcaatcagg cgttgcagga gatttcaaaa acatcggaaa
     5221 agtccaaacg gatattttat tgaagatgga aaaatgccgt ctgaaacgga aggtgtttca
     5281 gacggcattt ttgtcggatg attaattatt cggagcggtt gaagccaaac ttcacgcggc
     5341 tgcggccctg atccggtata ttgtccaaat cgcgtcccgg attggcggcg gtgtcgccta
     5401 cggaaatatc ggagatgttt tccaaaatga tggcggacga caggtgttcg gaggtgcggt
     5461 aaaccattgc caagcccact tcttcggcag gagtggaaat cagctcgacg gtatccctgc
     5521 ttttgaaatt gttggagagg tcgacctgca tcgttttctt gcgtttgtag aggctcaaaa
     5581 ccgtgccttt gtccaaaccg tccgcctcgc ctttgtcgat ggtgatggtt tgaaactggc
     5641 cggcaatcct tgtgccttca aacacggaaa cgattttagc ctgaaccggg cgggacggtt
     5701 cgtgcggcat catgttgaag cggtcggtgt cttccggcat tttcatcagg tagtcgccct
     5761 gctgtatttc ggaaatggcg gtttcgacca ccagcggctg tatcgaaggg gtgcgcagcg
     5821 gggtaatcaa aggatgggtg cgggtatggt attcgttgtc tttcggccgt tctccagcct
     5881 gtttcgagcg ttgttcgagg acagagtcgg tatagtcgag ggagcgcacg atgccgctga
     5941 atgcgacttc ctgcccgagg aatttacccg tatccggatc ggtgatgttt ttattgattc
     6001 ggtaggtcag gtagcggccc ggctctttca ggcctttggt gtaaaccctg gtgcctttgg
     6061 tgtacagcag cctgccttcc gggcccgaga gcaggcgcgg cgcggcagcg gtttctttgc
     6121 gggaaacgat ttgcgggtgc cgcataaaga tgcggtagaa gttgacatcg atggcgggaa
     6181 taccgtatcc ggacacttcc ttatccggac tcattttgac gacggggatg ccgtctgtct
     6241 gttccaagcc gaggcgcggt tcgccgtcaa cgtggcgcaa caccaatacc tggtccggat
     6301 aaatcaggtc gggattgtgg atttgatccc ggttcgcgtc ccacaggcgg ccccattgcc
     6361 acgggctgta caggtatttg cccgaaatgc cccacagggt gtcgccctgt ttgaccgtgt
     6421 agcgttccgg cgcgttcggg cgcacctcca aatttgccgc caaagtttgt gttgagaatg
     6481 ccatacctgc cgcgcagagc agggttataa tacgacgttg cataaccgtt ccccttatct
     6541 gataaatttc ggtttgtctt gcttgattgg gttggaaaaa gcggcggcag cccctcggga
     6601 tgtgccgcgt gataaaaaat gttccgcatt ttaacatcga attatccgca ccatcacggt
     6661 aattatgaaa aacaggcggc gtatccgccg aaggaaagag aaaattatgg ctttattgaa
     6721 tatcttgcaa tatcccgacg agcgtctgca cacggtggca aagcctgtcg aacaagtcga
     6781 cgagcgcatc cggaagctga ttgccgatat gtttgaaacg atgtacgaat cgcgcggcat
     6841 cgggctggcg gcgacgcagg tcgatgtgca cgagcgcgtg gtcgtgatgg atttgaccga
     6901 agaccgcagc gaaccgcgcg tgttcatcaa ccccgtcatc gttgaaaaag acggcgaaac
     6961 cacttacgaa gagggctgcc tgtccgtgcc gggcatttac gacaccgtaa cccgcgccga
     7021 acgcgtcaag gtcgaggctt tgaacgaaaa aggcgaaaag ttcacgctgg aggcggacgg
     7081 cttgctggcg atttgcgtgc agcacgagtt ggaccacctg atgggcatcg tgtttgtcga
     7141 acgcctttcc caactcaagc aggggcggat taagaccaag ctgaaaaaac gtcagaaaca
     7201 tacgatttga cccttttgcc gtgccgtctg aacgctgcaa agttttcaga cggcacggtc
     7261 ttgtccgaca attttacgca cacgcaggaa cacgctatga aagtcatctt cgccggcacg
     7321 cccgattttg ccgccgccgc cttaagagcc gttgccgccg ccggttttga aatcccgctg
     7381 gtgctgaccc agcccgaccg tccgaaaggg cgcggtatgc aactgactgc cccgcccgtc
     7441 aaacaagccg cgctggaact cggtttgcgc gtcgaacagc ccgaaaaact gcgtaacaac
     7501 gccgaagccc tgcaaatgct caaagaggtc gaggcagacg taatggtggt tgccgcctac
     7561 ggtttgattc tgccgcaaga cgtgttggat gtgccgaaac acggctgcct caacatccac
     7621 gcttcgctgc tgccccgttg gcgcggcgcg gcgccgattc agcgcgcgat tgaggccggc
     7681 gatgccgaaa ccggcgtgtg cattatgcag atggatgccg gtttggacac cggtgatgtg
     7741 gtcagcgaac accgctacgc catccaaccg accgataccg ccaacgaagt ccacgacgcg
     7801 ctgatggaaa tcggtgcggc ggcggttgtt gccgatttgc aacagcttca aagcaaaggc
     7861 cgtctgaacg cggtcaaaca gcccgaagaa ggtgttactt acgcgcaaaa attgcgcaaa
     7921 gaagaggcgc gtatcgattg gagcgaaagc gcggcggtta tcgaacgcaa aatccgcgcc
     7981 ttcaaccccg tgcctgccgc gtgggttgag tatcagggca agccgatgaa aatccggcgg
     8041 gcggaagtgg tggcgcaaca aggcgcggca ggcgaagtgt tgtcctgttc ggcggacggt
     8101 ttggtcgttg cctgcggcga aaacgcgctg aagattaccg aattgcagcc tgccggcggc
     8161 aggcgtatga atatcgcggc gtttgcagca ggacggcata tcgaagcagg gacgaagctg
     8221 taaatccctt cagacggcat tccgatccgc aaacgggaat gccgtctgaa accatcagtc
     8281 gaagaaagcg aatcacataa tatgagtatg gcacttgccc aaaaacttgc cgccgacagc
     8341 attgcggcgg ttgccgaagg acgcaacctt caggacgtgt tggtgcacat ccgcgcggcg
     8401 catcccgacc ttacggcgca ggaaaacggc gcgttacagg acattgccta cggttgccag
     8461 cgttatttgg gcagtttgaa acatatgctc gcgcagatgc tgaaaaagcc gattggcaat
     8521 ccgcagctcg aaagtctgct tttggcggcg ttgtaccaac tgcattacac gcgcaacgcg
     8581 ccgcatgctg tggtcaacga agctgttgaa agcatcgcca aaatcggacg cgggcagtac
     8641 cgttcgtttg ccaacgcggt tttgcgccgc tttttgcgcg aacgcgacaa gctcgcggct
     8701 tcctgtaaaa aagacgatgt ggcgaaacac aacctgccgc tgtggtgggt tgcctacctg
     8761 aaaaaccatt atccgaaaca ctggcacaac atcaccgccg cgctgcaatc gcatccgccg
     8821 atgacgttgc gcgtcaaccg ccggcacggc aatgccgaaa gctatttgga aaaactggct
     8881 gcggaaggta tcgccgcaaa ggcgttggac gaatatgcgg ttacgttgga agaagccgtg
     8941 ccggtaaacc gtctgcccgg tttttcagac ggcattgttt cggtacagga cttcggcgcg
     9001 cagcaggcgg cgtatttgtt aaacccgaaa gacggcgaac ggattttgga cgcgtgcgcc
     9061 gcgccgggcg gcaagacggg gcatatcttg gaactggcgg attgccgcgt taccgccttg
     9121 gacattgatg caggccgtct gaaacgggtg gaggacaata tcgcccgttt gggctttcag
     9181 acggcatcgg cggcgtgtgc cgatgcgcgg gatttggcgg cgtggtatga tggaaagcct
     9241 ttcgacgcaa tccttgccga cgtgccatgc accgcttccg gcgtggcgcg gcgcaatccc
     9301 gacgtgaaat ggctgcgccg tccgaccgac gcgctcaaaa ccgcccgcca gcaggaagcc
     9361 ctgctggacg cactttggca gacgctgaaa cagggcggca gaatgctgct tgccacctgt
     9421 tccgtgttcg tcgaagaaaa cgacgggcaa ttgcaaaaat tcctcaaccg ccatgccgat
     9481 gccgaaccga tcgaatcgcg ggtgctttta ccgaacaaac accaagatgg cttttattac
     9541 gcgcttattc aaaagcatta aacaatggct tgtgctgctg ccgatgcttt ccgttttgcc
     9601 ggacgcggcg gcggagggga tagatgtgag ccgcgccgaa gcgaggataa ccgacggcgg
     9661 gcagctttcc atcagcagcc gcttccaaac cgagctgccc gaccagctcc aacaggcgtt
     9721 gcgccggggc gtgccgctca actttacctt aagctggcag ctttccgccc cgataatcgc
     9781 ttcttatcgg tttaaattgg ggcaactgat tggcgatgac gacaatattg actacaaact
     9841 gagtttccat ccgctgacca accgctaccg cgttaccgtc ggcgcgtttt cgacagacta
     9901 cgacaccttg gatgcggcat tgcgcgcgac cggcgcggtt gccaactgga aagtcctgaa
     9961 caaaggcgcg ctgtccggtg cggaagcagg ggaaaccaag gcggaaatcc gcctgacgct
    10021 gtccacttca aaactgccca agccttttca aatcaatgca ttgacttctc aaaactggca
    10081 tttggattcg ggttggaaac ctctaaacat catcgggaac aaataatgcg ccgttttcta
    10141 ccgatcgcag ccatatgcgc cgtcgtcctg ttgtacggac tgacggcggc aaccggcagc
    10201 accagttcgc tggcggatta tttctggtgg attgttgcgt tcagcgcaat gctgctgctg
    10261 gtgttgtccg ccgttttggc acgttatgtc atattgctgt tgaaagacag gcgcgacggc
    10321 gtattcggtt cgcagattgc caaacgcctt tccgggatgt ttacgctggt tgccgtactg
    10381 cccggcgtgt ttctgttcgg cgtttccgca cagtttatca acggcacgat taattcgtgg
    10441 ttcggcaacg atacccacga ggcgcttgaa cgcagcctca atttgagcaa gtccgcattg
    10501 aatctggcgg cagacaacgc ccttggcaac gccatccccg tgcagataga cctcatcggc
    10561 gcggcttccc tgcccgggga tatgggcagg gtgctggaac attacgccgg cagcggtttt
    10621 gcccagcttg ccctgtacaa tgccgcaagc ggcaaaatcg aaaaaagcat caacccgcac
    10681 aagctcgatc agccgtttcc aggtaaggcg cgttgggaaa aaatccaacg ggcgggttcg
    10741 gtcagggatt tggaaagcat aggcggcgta ttgtacgcgc agggctggct gtcggcgggt
    10801 acgcacaacg ggcgcgatta cgccttgttt ttccgtcagc cggttcccaa aggtgtggca
    10861 gaggatgccg tcttaatcga aaaggcaagg gcgaaatatg ctgagttgag ttacagcaaa
    10921 aaaggtttgc agaccttttt cctggcaacc ctgctgattg cctcgctgct gtcgattttt
    10981 cttgcactgg tcatggcact gtatttcgcc cgccgtttcg tcgaacccgt cctatcgctt
    11041 gccgaggggg cgaaggcggt ggcgcaaggc gatttcagcc aaacccgtcc tgttttacgc
    11101 aatgacgagt tcgggcgttt gaccaagttg ttcaaccaca tgaccgagca gctttccatc
    11161 gccaaagaag cagacgagcg caaccgccgg cgcgaggaag ccgccagaca ttatcttgaa
    11221 tgcgtgttgg aggggctgac cacgggcgtg gtggtgtttg acgaacaagg ctgtctgaaa
    11281 accttcaaca aagcggcgga acagattttg gggatgccgc ttacccccct gtggggcagc
    11341 agccggcacg gttggcacgg cgtttcggcg cagcagtccc tgcttgccga agtgtttgcc
    11401 gccatcggcg cagcggcagg tacggacaaa ccggtccatg tgaaatatgc cgcgccggac
    11461 gatgccaaaa tcctgctggg caaggcaacc gtcctgcccg aagacaacgg caacggcgtg
    11521 gtaatggtga ttgacgacat caccgttttg atacacgcgc aaaaagaagc cgcgtggggc
    11581 gaagtggcaa aacggctggc acacgaaatc cgcaatccgc tcacgcccat ccagctttcc
    11641 gccgaacggc tggcgtggaa attgggcggg aagctggatg agcaggatgc acaaatcctg
    11701 acgcgttcga ccgacaccat cgtcaaacag gtggcggcat tgaaggaaat ggtcgaagca
    11761 ttccgcaatt atgcgcgttc cccttcgctc aaattggaaa atcaggattt gaacgcctta
    11821 atcggcgatg tgttggcatt gtatgaagcc ggtccgtgcc ggtttgcggc ggagcttgcc
    11881 ggcgaaccgc tgatggtggc ggcggatacg accgccatgc ggcaggtgct gcacaatatt
    11941 ttcaaaaatg ccgccgaagc ggcggaagaa gccgatgtac ccgaagtcag ggtaaaatcg
    12001 gaagtggggc gggacggtcg gattgtcctg acggtttgcg acaacggcaa agggttcggc
    12061 agggaaatgc tgcacaacgc cttcgagccg tatgtaacgg acaaaccggc gggaacggga
    12121 ttgggtctgc ccgtggtgaa aaaaatcatt gaagaacacg gcggccgcat cagcctgagc
    12181 aatcaggatg cgggtggggc gtgtgtcaga atcatcttgc caaaaacggt aaaaacttat
    12241 gcgtagcagc gatattttaa ttgtagacga cgaaattggc atccgcgacc tgctgtcgga
    12301 aatcctgcag gacgaaggtt attcggtcgc attggcggaa aacgccgaag aggcgcgcaa
    12361 gctgcgccat caggtacgcc cggcgatggt gttgctggat atttggatgc ccgattgcga
    12421 cggcatcacc cttttgaagg agtgggcgaa aaacgggcag ctcaatatgc cggtggtgat
    12481 gatgagcggg catgccagca tcgataccgc cgtagaagcg accaaaatcg gcgcgctcga
    12541 ctttttggaa aaaccgattt ccctgcaaaa gctgctgtct gccgtcgaaa acgcgttgaa
    12601 acacggtgcg gcgaaaatcg aaacgggacc ggtgttcgac aagctgggca acagtgcggc
    12661 gattcaggaa atgaaccgtg aggtaggggc tgcggtgaaa cgtgcctctc ccgtactttt
    12721 gacgggcgag gcaggttcgc cgtttgaaac ggtggcacgc tatttccata aaaacggtac
    12781 gccgtgggtc agcccggcaa gggtcgaata tctgatcgat atgccgatgg aactgttgca
    12841 gaaggcggag ggcggcgttt tgtatgtcgg cgacatcgcc cagtacagcc gcaacatcca
    12901 agccggtatt gcctttattg tcggcaaggc ggaacaccgc cgcgtcaggg tggtcgcatc
    12961 gggcagcagg gcggcaggtt cagacggcat tgcctgcgag gaaaagctgg cggaactgct
    13021 gtcggagtcg gtcgtccgca ttccgccgct gcgtatgcag catgaagata ttcccttcct
    13081 gatacagggg attgcctgca atgtggcgga aagtcaaaag attgcgcccg ccgcattcag
    13141 tgaagaggca cttgccgcat tgacccgtta cgactggccg ggaaatttcg accaactgca
    13201 aagcgtcgtt gcaacgctgt tgttggaggc ggacggacag gaaatcggcg caggggcggt
    13261 ttcttccctt ttggggcaga atgtgcctgc cgagggagcg gaagatatgg tgggcgggtt
    13321 taatttcaac ctgcccctgc gcgaattgag ggaggaggtg gagcggcgtt atttcgagta
    13381 ccacatcgcc caagaaggtc agaatatgag caaagtggcg cagaaagtcg gtttggaacg
    13441 cacgcacctt taccgcaaac tcaaacagct cggcatcggc gtttcgcgcc gggcggggga
    13501 aaaaaccgaa gaataggccc ggacggccgg ttttccggct gcgggctttt gttttcagac
    13561 ggcatttggt gcaaatgccg tctgaaatcg taaggggacg gattttatga cagaggacga
    13621 acgtttcgcg tggctgcaat tggcgtttac gccctatatc ggcgcggaaa gtttcctgct
    13681 gctgatgcgc cgtttcggca gcgcgcaaaa tgccctgtcc gcaccggcgg aacaggtggc
    13741 ggcactgata cggcacaaac aggcgcttga ggcttggcgc aatgcggaaa aacgcgcttt
    13801 ggcgcggcag gcggcagaag cggcgttgga atgggaaatg cgggacggat gccgcctgat
    13861 gctgcttcag gatgaagatt tccccgaaat gctgacgcag gggctgaccg cgccgccggt
    13921 tttgtttttg cgcggcaacg tgcggctgct gcacaagcct tccgccgcca tcgtcggcag
    13981 ccgccatgcc acgccgcagg ccatgcggat tgccaaagat ttcggcaggg cgttgggtga
    14041 aaaaggcatt cccgttgtgt cgggtatggc ttcgggcatc gataccgccg cccatcaggg
    14101 cgcactggag gcagaaggcg gcaccatcgc tgtgtggggg acgggcatag accgcattta
    14161 tccgccgtcc aataaaaacc ttgcctatga aatcgccgaa aaagggctga ttgtcagcga
    14221 gttccccatc ggtacgcggc cgtatgccgg caactttccg cgccgcaacc gcctgattgc
    14281 cgcgctgtcg caggttacgc tggtggttga agccgccttg gaatccggtt cgctgattac
    14341 cgccggcttg gcggcagaaa tggggcgcga agtgatggcg gtacccggtt cgatagacaa
    14401 tccgcacagc aaaggctgcc acaaactgat taaagacggc gcgaaattgg tggaatgcct
    14461 ggacgacatc ctgaacgaat gcccggggct attgcaaaat acgggtgctt catcatattc
    14521 tataaataag gatacgcccg acacgggcag tcgcgccgtt cagacggcat acgccccgcc
    14581 gcctgccgca aaaatgccgt ctgaaggggc ggcaggcggc acagccgtcg gcggcatact
    14641 ggataaaatg ggtttcgacc ccgtccatcc cgacgtgctt gccggacagt tggctatgcc
    14701 tgccgcagat ttgtatgccg cactgttgga attggaattg gacggcagcg ttgccgcaat
    14761 gcccggcggc agataccagc gtatccgaac ttgaacgcac tttatattaa ggaacacgaa
    14821 tgaccgaagt catcgcctac ctcatcgaac atttccaaga tttcgatacc tgtccgccgc
    14881 ccgaagactt gggtatgctg cttgaagaag cgggtttcga taccatggaa atcggcaaca
    14941 ccctgatgat gatggaagta ttgctcaaca gctccgaatt ttccgccgaa cctgccgaca
    15001 gcggcgcatt gcgcgtgtac agtaaagaag aaaccgacaa cctgccgcag gaagtgatgg
    15061 ggctgatgca gtatctgatt gaagaaaaag ccgtcagctg cgaacagcgg gaaatcatca
    15121 tccacgcgct catgcacatt ccgggcgacg aaattaccgt agataccgcc aaagtgctga
    15181 ccctgctgct tttatgggca aacaagagcg agctgcccgt gttggtcggc gacgagctga
    15241 tgagcgcgct tttactcgac aacaaaccca cgatgaactg aagcggcttc agacggcccg
    15301 cccgaggccg tctgaaacgt cggcatcaaa accaccatcc agagaacgac aaatggcgaa
    15361 aaacctatta atcgtcgaat ccccgtccaa agccaaaacc ctgaaaaaat atttgggcgg
    15421 cgattttgaa atccttgcat cctacggaca cgtccgcgac ctcgtcccca aaagcggcgc
    15481 ggtcgatccc gacaacggct ttgcgatgaa ataccaattg attagccgca acagcaaaca
    15541 cgtcgatgcc atcgttgccg gtgccaaaga agctgaaaac atctacctcg ccaccgaccc
    15601 ggatagggaa ggcgaagcca tttcctggca tcttttggaa atcctcaaat ccaaacgcgg
    15661 cttgaaaaac atcaagccgc agcgtgtcgt gttccacgaa atcaccaaaa acgccgtgct
    15721 cgatgccgtt gcccatccgc gcgaaatcga aatggacttg gtcgatgcac aacaagcccg
    15781 ccgtgctttg gactatttgg tcggctttaa cctttcgcca ttgttatgga aaaaaatccg
    15841 ccgaggtttg agcgcggggc gtgtgcaaag ccctgctttg cgcctgattt gtgagcgcga
    15901 aaacgaaatc cgcgcgtttg aagcgcagga atattggacg gtgcatctcg acagccacaa
    15961 aggccgcagc aagttcaccg ccaaactcgc ccaatacaac ggcgcgaaac tcgaacaatt
    16021 cgacctgtcg aacgaagccg cgcaagccga cgtattgaaa gaactcgaag gcaaagaagc
    16081 cgtcgttacc gccattgaaa agaaaaagcg cagccgcaat cctgccgcgc cgttcaccac
    16141 ttctaccatg cagcaggatg ccgtgcgcaa actcggcttc accaccgacc gcaccatgcg
    16201 taccgcccaa cagctttatg aaggtataga cgtagggcag ggcgcgatcg gtctgattac
    16261 ctatatgcgt accgacagcg tgaatttggc ggatgaagcg ttaaccgaaa tccgccatta
    16321 cattgaaaac aaaatcggca aagaatatct gccgagtgcc gccaaacaat acaaaaccaa
    16381 atccaaaaac gcccaagaag cgcacgaagc catccgtccg acttccgtgt accgcacgcc
    16441 cgaaagcgtc aaacccttcc tgagcgcaga ccagttcaaa ctctatcaaa tgatttggca
    16501 gcgtaccgtc gcctgtcaga tgacgcctgc caaattcgac caaaccaccg tcgatattac
    16561 cgtcggcaaa ggcgtattcc gcgtaaccgg acaagtgcaa accttcgcag gcttcttgag
    16621 cgtttatgaa gaaagcagcg acgatgaaga aggcgaagac agcaaaaaac tgcccgaaat
    16681 gagcgaaggc gataaattgc ccgtggacaa actctacggc gaacaacact ttaccactcc
    16741 gccgccgcgc tacaacgaag ccacgctggt taaagccctc gaagaatacg gcatcggccg
    16801 cccctcgacc tacgccagca tcatctccac gctcaaagac cgcgaatacg ttacccttga
    16861 gcaaaaacgc ttcatgccca ccgacacagg cgacatcgtc aacaaattcc tgaccgaaca
    16921 cttcgcccaa tacgtcgatt accacttcac cgccaaactc gaagaccagc ttgacgaaat
    16981 tgccgacggc aaacgccaat ggattccctt gatggacaaa ttctggaaac cgttcatcaa
    17041 gcaagtggaa gaaaaagaag gcatcgaacg cgccaaattt accacgcagg aacttgatga
    17101 aacctgcccg aaatgcggcg aacacaaact gcaaatcaaa ttcggcaaaa tgggtcgttt
    17161 tgttgcgtgt gccggttatc ccgagtgcag ctacacgcgc aatgtcaacg aaaccgccga
    17221 agaggccgcc gagcgcatcg ccaaagccga agccgaacag gccgaactcg acggacgcga
    17281 gtgcccgaaa tgtggcggtc gcctagtgta caaatacagc cgcaccggca gcaaattcat
    17341 cggctgcgcc aactatccga aatgcaaaca cgtcgagccg ctggaaaaac cgaaagatac
    17401 cggcgtccag tgtccgcaat gcaaaaaagg caacctcgtc gagcgcaaat cccgctacgg
    17461 caaactgttt tacagttgca gcacctatcc cgactgcaac tacgccactt ggaacccgcc
    17521 cgttgccgaa gaatgcccga actgccattg gccggtcttg accatcaaaa ccaccaaacg
    17581 ctggggcgtc gaaaaagtct gcccgcaaaa agaatgcggc tggaaagaac agattgaacc
    17641 gcccgcgccg aaggagtaag attaggttgg tttgaaagag aaaaggtcgt ctgaaaaatt
    17701 ttcagacgac ctttgctttt ctgtgattgg tttatttgaa tccgcgtgtt gttttaaagt
    17761 ccgataaaat ccggttcatt tcaggcacaa acaaggcgat gtaatcgtaa gatagaccgc
    17821 gactggcact gggatgggga aagcagacga cttcgcaatc ttcaaacgat tggaatttga
    17881 cattgaaacg tgtaccgtca aattcttttt gcaccgtctc cagcggtttg gtctgcttac
    17941 cgaccaactg ctcgaagcgt ggcagtacat tttggttgtt cagaaaatcc gccaacctgc
    18001 tgcccatgaa gaggatgact ttcggacgca gtttttcgat gtggtagaga aaattatcga
    18061 tgtgctcggg ttgtgtgaac ttgtcgggat tgtcgatagt gttgccctgt gtagcagccc
    18121 agttggtttg aaccagggat ttttcaaatg caccgcccaa tccattttcg tctaaggggt
    18181 gtccccacat ttcaaaccaa ttttttatcg tattgtcgta acgccacttc tttgcctgct
    18241 ctccgaaata gagggatttg tttgcaaatg tatggtcgat tttgttttca gggagtttgt
    18301 attcacctgc tacataagca gcctcatcgg ctttactcca accccattca tacccacaaa
    18361 tcattaaacc atgtttgtcg ttgtagcctt tgaacagact gttgctcaaa ttcaaatcct
    18421 tcatcatgaa ctcttccttt taaaatttaa gagcgattga cttcaatgtt tttagatggg
    18481 gtggaaaaat ccttgtgtag gcaacataaa ttcaataaat ttcttgataa ttcgaaacct
    18541 actaatagcg cacctataaa agctttttca ttacgttcag catgacggtc acgtcgttca
    18601 tattttttac gcttgctgtt ccctgttatt acagctaagc caagtgatat ggcgagaatt
    18661 gcccaaacaa tagtacttaa ataacaattt tccccatact atcaataagg aaagaaaaaa
    18721 ccttttagta ttaggtcgat agattataat ccatgcccat gaaaatgcaa taagagttat
    18781 acataagata caagctgaca ggatttcttt atttttaatt aaataactta gagcgatgag
    18841 gatgacaggt gtcaagaaaa taatagttac atcccgatag ctataaaaga aaactgccct
    18901 attttgaatg tggagatgtg cacagaatcc aatatagcta aggataatag ttaatataat
    18961 aaaaaaagac caccaagggt gaagagatag gaattccatg ttttcccttt tttgtaaaaa
    19021 ggaaaaaatc ttatctaata gttaattgcc taaccagcca gaagaagttt aaaatctatc
    19081 ccaataattc aaccatctat acagaaagtt cagcttatgg aaacccacga aaaaatccgc
    19141 ctgatgcgcg aattgaataa atggtcccag gaggatatgg cggaaaagct ggcgatgtcg
    19201 gcaggcgggt atgccaaaat cgaacggggc gaaacgcagt tgaatatccc gcgtttggag
    19261 cagttggctc agattttcaa aatcgatatg tgggacttgc tcaaatcggg cggtggtggg
    19321 atggtgtttc agattaatga aggtgatagt ggtggcgata ttgcgttgta tgcgtcgggt
    19381 gatgtttcga tgaaaataga atttttaaaa atggagttga aacactgcaa agaaatgttg
    19441 gaacaaaaag acaaagaaat cgagctgctc cgcaagctga ccgaaaccgt ttaaacagat
    19501 atgccgtctg aaaaaagttt tcagacggca tattctttga caggtcttgt ataataccgt
    19561 ttgaacttac aggtttttga ttatggcggc aggcaaacat accaaacaca gcaaccgggt
    19621 acgcattatc ggcgggcaat gccggggcag gaaattgagt ttcacatccg ccgacggact
    19681 gcgtccgaca cccgacagcg tgcgtgaaaa gctgtttaac tggctgggac aggatttgac
    19741 gggtaaaacg gttttggatc tcttcggagg cagcggcgca ctcggtatag aagccgcttc
    19801 gcgcaacgcc aaacgcgtgc tgatttcgga taacaaccgc caaaccgtgc agaccttgca
    19861 gaaaaacagt cgcgaactgg gtttggggca ggtgcaaatc gtcttttcag acggcatcgc
    19921 atatttgaag accgtatccg aacagtttga tgttgtcttt ctcgacccgc cgtttgcatg
    19981 gcaggactgg caaatcctgt tcgatgcctt gaagccgtgc ctgaaccccc gggcattcgt
    20041 ctatctcgag gcgggtaggc tgccggatat ccccgattgg ctgacggaat atagagaggg
    20101 gaaatcgggg cagagtacat ttgaattaag ggtttttcaa gtggctgaat aatatgcgct
    20161 ttgataatca tttccgagtt gtaaacattc gtttgcaacc gtccggttca aaaaaacctt
    20221 gtgctataat ccgcgcccgc ccggttttga taatttagtg gaaaaggaaa agaaatgtcg
    20281 ctttttatta ccgacgagtg catcaactgc gacgtatgcg aacccgaatg ccccaatgat
    20341 gccatttccc aaggcgagga aatttacgaa atcaacccca acctctgcac gcagtgcgtc
    20401 ggacactacg atgagccgca gtgccagcag gtttgcccgg tggactgcat cctgattgac
    20461 gaagaacatc ccgaaaccca tgacgagttg atggcgaaat acgaaaagat tatccagttt
    20521 aaataaattc tttttaaaac atcaaattat gtctgttttg aaataaaatc aaaaaaaaac
    20581 ttgacggaaa agcaagccgc taataaacta acgttctctt ttggagggat tcccgagcgg
    20641 tcaaaggggg cagactgtaa atctgttgcg aaagcttcga aggttcgaat ccttctccct
    20701 ccaccaaaat tcttacttgg ggcagtagcg agtaatgcgg gtgtagctca atggtagagc
    20761 agaagccttc caagcttacg gtgagggttc gattcccttc acccgctcca aacaattagg
    20821 cccatgtagc tcaggggtag agcactccct tggtaaggga gaggccggca gttcaaatct
    20881 gcccatgggc accatctctc gattattcat ttctttaagg cttagatata taggatattg
//
LOCUS       DAWFJW010000023        20901 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFJW010000023 DAWFJW010000000
VERSION     DAWFJW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20901
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.023"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(1..682)
                     /locus_tag="WGR19_000716"
                     /pseudo
     CDS             complement(1..682)
                     /locus_tag="WGR19_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(768..1190)
                     /locus_tag="WGR19_000717"
     CDS             complement(768..1190)
                     /locus_tag="WGR19_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798281.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNFQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            1502..2812
                     /locus_tag="WGR19_000718"
     CDS             1502..2812
                     /locus_tag="WGR19_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0798282.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            2926..5931
                     /locus_tag="WGR19_000719"
     CDS             2926..5931
                     /locus_tag="WGR19_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0798283.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSAEQARQLFEHFQIASDHNFTAALILHDVGTKDERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSRLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIADKK
                     QLLALKKALQTAKKLYNVLRLQGSHEFLAHLDFDKLNLLYRETAARLETLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQEDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIAILETVYAKIKELNRQNDLLRHKYGGDAKYARIH
                     KRLRENPALYGDKLKVFNALNGVKTDTDQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            6056..7213
                     /locus_tag="WGR19_000720"
     CDS             6056..7213
                     /locus_tag="WGR19_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0798284.1"
                     /translation="MNSALKNYAAYVNNKIDVCEISLNQYISTDNILQNKQGIECAAS
                     LPIQGGKVTAFKKGDILLANIRPYLKKIWYAQFDGGCSADVLAIRANAKIDSHFLFYA
                     LFRDDFFIHAMKGSKGTKMPRGDKTQIMEFKIPVFAPQTQQSITTVLSALDKKIALNK
                     QINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEMVFDETLKREIPKGWESVELQ
                     SCLAKVPSTVKISNKDIKDFGKYPVIDQSQDFICGFTDDEKSILNPQDAHIIFGDHTR
                     IVKLVNFKYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHFKFLKEFPII
                     LPDKDISRKYYEIVNYFFIKIRNNIKQNHHLTQLRDFLLPMLMNGQVSVAE"
     gene            7262..8863
                     /locus_tag="WGR19_000721"
     CDS             7262..8863
                     /locus_tag="WGR19_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0798285.1"
                     /translation="MTEQHFTEQTKVLIDSLKTICAHYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKVRKENPNDPIEFVNMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEDVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISAEEFEQKMAEFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            8874..9011
                     /locus_tag="WGR19_000722"
     CDS             8874..9011
                     /locus_tag="WGR19_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0798286.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(9171..10508)
                     /gene="ribB"
                     /locus_tag="WGR19_000723"
     CDS             complement(9171..10508)
                     /gene="ribB"
                     /locus_tag="WGR19_000723"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0798287.1"
                     /translation="MTDTADLRRRNLRQWIEKHYGGLQTRFAEAVCLNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPSAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHNLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPTADEETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRAEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLIGFGLEVVGFEEAEK"
     gene            complement(10723..11734)
                     /locus_tag="WGR19_000724"
                     /pseudo
     CDS             complement(10723..11734)
                     /locus_tag="WGR19_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(11902..12495)
                     /gene="ribA"
                     /locus_tag="WGR19_000725"
     CDS             complement(11902..12495)
                     /gene="ribA"
                     /locus_tag="WGR19_000725"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0798288.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(12488..13162)
                     /locus_tag="WGR19_000726"
     CDS             complement(12488..13162)
                     /locus_tag="WGR19_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798289.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSATVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            13316..13400
                     /locus_tag="WGR19_000727"
     tRNA            13316..13400
                     /locus_tag="WGR19_000727"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13350..13352,aa:Leu,seq:tag)
     gene            13920..14954
                     /gene="purM"
                     /locus_tag="WGR19_000728"
     CDS             13920..14954
                     /gene="purM"
                     /locus_tag="WGR19_000728"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0798290.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(15428..16084)
                     /locus_tag="WGR19_000729"
     CDS             complement(15428..16084)
                     /locus_tag="WGR19_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0798291.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(16081..17853)
                     /locus_tag="WGR19_000730"
     CDS             complement(16081..17853)
                     /locus_tag="WGR19_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0798292.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            18059..18673
                     /locus_tag="WGR19_000731"
     CDS             18059..18673
                     /locus_tag="WGR19_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0798293.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFLDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQIDLQEG"
     gene            18836..19453
                     /locus_tag="WGR19_000732"
     CDS             18836..19453
                     /locus_tag="WGR19_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0798294.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            19461..20324
                     /locus_tag="WGR19_000733"
     CDS             19461..20324
                     /locus_tag="WGR19_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0798295.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(20337..20771)
                     /locus_tag="WGR19_000734"
     CDS             complement(20337..20771)
                     /locus_tag="WGR19_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0798296.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
BASE COUNT         5454 a         4978 c         5247 g         5222 t
ORIGIN      
        1 cgcaaatgcc gttttgtaca attccaaaag ttttcaattc cgttaatgcg attttgccgt
       61 ttggcgaaat gcgtactgtt ccagtcgtgg attgaacccc caccctgtat agttctttcg
      121 aagcattggg gtattgtttt ttcaaagcat cgattaaagc cgtattggtg tccggatttt
      181 taacctggac aatattgtcc ccttcggctt caagcgttgc ttttgcagaa tgcgacgcga
      241 taatgccttt tacggaggcg tggtttttta catttaaatc aacttcatta tttcccttga
      301 cggctttgag tttgatggtg ctgatgccat caagggaagg ttgggtaagg tcgcttgatt
      361 gattattgat gaaaatgccc gtcgtgtgtc cggaataggg ggagttggac tggagaccat
      421 ttacgcccaa tcccaagccc ttgccctcag tggcgatgga tattttattg ttattccccg
      481 ttaatacggt attggattta aaagccgttt caatgcccct taaaaaactt gtactctcaa
      541 catttcctgt cggcataggg tcggcgagtt cccctttaat ctcaatcaca ttatcgctgt
      601 cttcggcagt cagatgaaca tcggtattgt tgaggttgtg aataccgaaa tggtgtgtgt
      661 ctttatgaac tttaacgttc atattgaaca aattgcctgt ttttgaatga aaggatatgc
      721 tgcctttgtc attttcatcg gggggctgat agtgttgaca taccgcacta aggcactatt
      781 gtttgtcggt atttttttcc actggctaca tcttgtaccc catcaacatc gggaacattc
      841 aaattaaatt gaatttttcc tttggcatcc aaatggatat ttccggaatg gggcttcacg
      901 tcaacagatt ggaaattatt tttaaataga atatcattat tgctttttac caataattca
      961 ggcgtatatc ccaattgaga gtctgcaaaa gattggatgc cgtaaattcc atttaccgtt
     1021 aaatttccat tatcattttt accagtggta ataagttcta attttctaaa gttttcacct
     1081 tttttccacc aaggcacaat atagtttttt acagtgattt tttcatcttt aagggttata
     1141 agactatcat cgtctctttg cggatcagaa aataaagaca cccattccat ctcttgaata
     1201 ttagtatatt catgggcttc cgcctcaaaa cataatgccg attgaatcaa caaagcaatg
     1261 gtagataaag caaattttct attttagtct ccttaatgtt taattgaaac gctgatttgg
     1321 gtattgtcat gccggtgcgg aatcagtctc cgtgccggat atattgtgtg aagtctctcg
     1381 tcttttccag tatgcgccta atatcggctt aaggcaaacg gaggggcgga atccgtatgc
     1441 ggcacgccgc cgttccctgc cgtataatca tattgtttga aaacctgttg acggagccgt
     1501 tatgaccgat ttgtttgccc gagaacccga cgcgccgctt gccgaacgat tgcgtccgca
     1561 tacgcttgac gacgtggtgg ggcaggaaca cctcatcggc gaaggtaaac ctttgcgcgt
     1621 ggcggtagaa ggcggcaagc cgcattctat gttgctgtgg gggccgccgg gcgtgggcaa
     1681 gacgacgttg gcgcggattt tggcgcagag tttcaacgcc cagtttttgc ctgtttccgc
     1741 cgtattttcc ggcgtgaagg acatccgcga ggcaatcgat aaagccgaaa tcgctttgca
     1801 gcagggacgc gcgacgattt tgtttgtcga tgaagtccac cgcttcaaca aggcgcagca
     1861 ggacgcgttt ttgccgcatg tcgaaagcgg tttgctaacc tttattggtg cgacgacgga
     1921 aaatccgtcg tttgaagtca atcccgcgct gttgagccgc gctcaggtgt atgttttgca
     1981 acccttgtct tcagacgacc tgaaaaagct gattgccaag gtattggctt tgcctgaata
     2041 ccaagagttt acgattgaaa cggatgcgca aaaattactc gtgaataccg ccgacggtga
     2101 tgcgcgcaga ttgttgaatt tgttggaaca acttttacgc gccgccgata cacgtcgtct
     2161 gaaaaactta accgccgaat ttctcgccga cagtctcggg gcgcaaatcc gccgtttcga
     2221 caaaggcggc gagagtttct acaaccaaat ctccgccctg cacaaatccg tgcgcggttc
     2281 gcatccgaac gccgcgctgt attggttctg ccgtatgctc gacggcggca ccgacccgcg
     2341 ctacctcgcc cgccgcatcg tgcgtatcgc ttgggaagac atcgggcttg ccgacccgcg
     2401 cgccttccaa atcgccaacg atgccgccgc caccttcgaa cgcttaggct cgcccgaagg
     2461 cgaactggct ttggcgcaag cggtattgta tcttgccgcc gccgcgaaat ccaacgcggg
     2521 ctacaaggca tacaaccaaa tgcgccgttt cgtcaaagaa aacgccagcg acgaagtacc
     2581 cgtccacctg cgcaatgccc cgaccaagct gatgaaggaa ttgggctacg gacgcgaata
     2641 ccgctacgcc cacgacgaac cgaacgccta cgccgccggc gaaagctata tgcccgacgg
     2701 cttggacgaa ccggacttct accaacccgt cccgcgcggg ttggaaatca aaatcggcga
     2761 aaagctggcg tggctcaaat cgctggatga agaggcatta aaggcaaaat gaagcaatgc
     2821 cgtctgaagc agaacagtat ggcaaagccg gtacaccgaa aaggcgtatc gggtttttca
     2881 ggtgttataa tccgtttcaa taaattttga tgaatcatta cgtttatgtc ccaaactcac
     2941 aacgaaaatt cccgcgtcaa aatccccgcc gttttgcacc tgatgcgctt gggctacgac
     3001 tacctctcgc tcaaaaacga aaattgggat aaaccaacca acatctttcc caaaatcttt
     3061 atagaaagcc tctgccgcat caatcccgat ttgtcgccgg acgatgcacg ccgtttgctg
     3121 accgacatac gcttagaact ggacaacgaa gacttggggc aaaagtttta cgaacgcctt
     3181 accaatcaat ccggcggcaa gaaactgatc gacttccaaa attttgataa caacagcttc
     3241 cacgttgtaa ccgaattgcc ctgtatcaac ggcgatgaag aattccgccc cgacattacc
     3301 ctgctggtaa acggcatgcc tttggttttt atcgaagtca aaaagcccaa caacaaaggc
     3361 ggaatcggcg aagagcggga gcgcatgggc aaacgtgcga aaaattccaa atttcgccgt
     3421 ttcatcaaca tcacccaatt catgattttt tccaacaaca tggaatacga cgacggcgca
     3481 accgaaccgg cacaaggcgc gttttacgct tcgtctgctt acggtaagcc cgtgtttaac
     3541 tacttccgcg aagaacataa attaaatctt gccgaattat tgaacacgct ttcagacggc
     3601 ctcgaaaaca acgtcctgca agacaacaac ctgcccgtta tcaaacacag ccccgaattt
     3661 atcagcaata aatcgcccga tacgccgacc aaccgcatcc tgacttcgct gctttgccgc
     3721 gaacgtcttt cttttcttct gcaacacggg ctgacttacg tcaaagcaag tcaaggcttg
     3781 gtgcaaaaac acatcatgcg gtatccgcag ctatttgcca ctctcgccat tgaaaaacat
     3841 ttggcaaacg gcggcaaaaa aggcgtgatt tggcacaccc aaggctctgg caaaaccgcg
     3901 cttgcctatt acaacacccg ctacctgacc cactattatg ccaaacaggg catcgtgccg
     3961 aaattctatt tcatcgtgga caggcttgat ttattgaaac aggcgcagcg cgaatttact
     4021 gcccgcgatt tagtcgtcca taccatcgac agccgcgaag cctttgctgc cgacatcaaa
     4081 tccgcccaaa ctttgcacaa ccacgcaggc aaagcggaaa tcactgttgt taatatccaa
     4141 aaattccaag acgaccccga tgtcgtcgct cgtaacgact acgacctcgc cattcagcgc
     4201 gtctattttc tcgacgaagt acaccgcagc tacaacccca aaggctcgtt tcttgccaac
     4261 ctcaaccagt ccgacgtaaa cgccgtcaaa atcgggctga ctggcacgcc gcttatcggc
     4321 gtaaccgcag gcaacgtcaa cacccgcgaa ctcttcggcg actacatcca caaatactat
     4381 tacaacgcct ccattgccga cggctacacc ctgcgcctga tacgcgaaga aatcggcagc
     4441 cgatacaagg cgcaattaca agaagcactg gcgcaacttg aaatcgaaaa aggcagcttt
     4501 gaccgcaaag aaatctacgc ccatccgcac ttcgtccacc cgatgcttga ctacatcttg
     4561 gacgacttcg ccaaattcag aaaaaccaac caagacgaca gcctcggcgc tatggttgtt
     4621 tgcgacagcg cggaacaggc acgtcagctt tttgagcatt ttcagatagc ctcagaccat
     4681 aatttcaccg ccgcgctgat attgcacgac gtcggcacta aggacgaacg cgaccaatgg
     4741 gttaaagatt tcaaagcggg caaaatcgac atcttgtttg tgtacaacat gcttttgacc
     4801 ggctttgacg cgccgcgcct gaaaaagctc tatttgggca gactgataaa agcccataac
     4861 cttttacaaa ccctgacccg cgtcaaccgc acctacaaat cttaccgcta cggctatgtt
     4921 gtcgattttg ccgacatcga gcgcgaattt gacaaaacca accgcgccta ttgggacgaa
     4981 ctttccaacg aattgggcga cgaaatcggc agctacagcc ggcttttcaa aaccgccgaa
     5041 gaaatcgaac aggaaattgc agacattaaa aacgccctgt ttgatttcga taccgaaaac
     5101 gccgaagaat tttgcagcca aattagccaa atagcagaca aaaaacagct gctcgcccta
     5161 aaaaaagccc tgcaaaccgc caaaaagctg tacaacgtcc tgcgcctgca aggcagccac
     5221 gaatttctcg cccatctcga ttttgacaag ctcaatcttt tgtaccgcga aaccgccgcg
     5281 cgtttggaga ccttaaacct tgccgaaaaa ctacagcaag gcgacaccgc ccatttgctc
     5341 aatgaagcgc tggaagacgt ttactttcaa ttcgttaaaa tcggcgaagc cgaactcaaa
     5401 cttgccgacg atttgaaaga catcatgcgc aaagtccgcg aagggctggc gggcaacttc
     5461 gatcaagaag accccgaata catcagcctg cgcgaagaac tggaacgcat cttcaagaaa
     5521 aagaacctcg ccgaagtcgg gcaagaagaa atgcaggcaa acatcgccat actcgaaacc
     5581 gtctatgcca aaatcaaaga gctgaaccgc caaaacgacc tcttgcggca caaatacggc
     5641 ggcgacgcca aatatgcccg catccacaaa cgcctgcgcg aaaaccccgc cctctacggc
     5701 gataaactca aagtctttaa cgcgctgaat ggtgtaaaaa ccgacacgga ccaaaaagtg
     5761 ctggatatgg agcagatttt ggacaaccaa aattactttg aaaaacaaat gcagggcatc
     5821 gtattaaaac gctttagaac ggaacagcaa tttcccgttc aacccgcaga catccaagcc
     5881 atcaaccgcc tgttggtgcg ggaatattta aaagaaagcg ggcggatttg aggaattggt
     5941 ttcaggcagc ctttacgggc gtattgcata cgttcgaagt ggacaataaa agtgcggtcg
     6001 gattttgaaa tgtttttcag acggcctttg aagagttttg agagataaaa tcaatatgaa
     6061 tagtgcacta aagaattatg cagcatatgt aaataataaa attgatgtat gtgaaatttc
     6121 gcttaaccaa tatatttcaa cagataatat cttgcaaaat aagcaaggaa tagagtgtgc
     6181 agcatcttta ccgattcaag gtggaaaagt aactgcattt aaaaagggtg atattttatt
     6241 agctaatata cgaccgtatc taaaaaaaat ttggtatgcc caatttgatg gaggttgttc
     6301 tgctgatgtt ttggcgattc gtgcaaacgc caaaatagat agtcatttct tattttatgc
     6361 tttgtttcgt gatgattttt ttatccatgc aatgaaaggt tcaaagggca caaagatgcc
     6421 gcgaggcgat aagacacaga ttatggagtt taaaatccct gtttttgccc ctcaaaccca
     6481 gcaatccatc accaccgtcc tatccgcttt ggacaaaaaa atcgccctga acaaacaaat
     6541 caacgcgcgc ttggaagaga tggcgaaaac cctgtacgac tactggtttg tacagttcga
     6601 ttttcccgac gccaatggca agccctacaa atcctcaggc ggcgaaatgg tgtttgacga
     6661 aaccttgaaa cgggaaattc cgaaggggtg ggaaagcgta gaattgcaat cttgcttagc
     6721 aaaagtccca agtactgtaa aaatatcaaa taaagacatt aaggattttg gaaaatatcc
     6781 tgttattgac caaagtcaag attttatttg cgggtttact gacgatgaaa aatctatatt
     6841 aaaccctcaa gatgctcata ttatttttgg tgatcataca agaatcgtta agttagttaa
     6901 ttttaaatat gcgcgagggg cggatggtac acaagtaata ttaagcaata atgagcgaat
     6961 gccaaactat ttgttttacc aaatcataaa tcaaatagat ttatcaagtt atggatatgc
     7021 tagacatttt aaattcttaa aagagtttcc aattattttg ccagataaag atatttctcg
     7081 aaaatattat gagatagtaa attatttctt tattaaaatt cggaataata taaaacaaaa
     7141 ccaccaccta acccaactac gcgatttcct actgcccatg ctgatgaacg ggcaggtatc
     7201 cgtggcggaa taagccggtt tcaagaatcc gagcgataca agaaaaagaa tgagataaaa
     7261 aatgaccgaa caacacttta ccgaacaaac caaagtcctg attgacagct taaaaaccat
     7321 ttgcgcccac tacggtttgg gtaatgacgg caacgaattt aaaatcatca gtcaggcgtt
     7381 tttgtataaa ttcttaaacg acaaatacga tttcgaagtc aaaaaagttc gcaaagaaaa
     7441 ccccaatgac ccgattgagt tcgtcaatat ggatattgag ggaaaaacag cggttttgaa
     7501 gcccgagcat tccatcaaat atttgtcgga gcggcaaaac ggcgcggact ttgccaaact
     7561 ctttgacgat acgctcactg acatcgccgc ttgtaatgcg gagttgtttt ccgtaaagac
     7621 agaaggcgga gcgaaaatcg tattgttcga gcgtatcagt caatatatta cggatgaagg
     7681 ccgccgcgat gatttttgcc gcgcgctgat ttccaaactg gcaggtttta gcttcgaagc
     7741 gatttttgcg caaaaatttg atttttttgc cacgattttt gaatacctga ttaaagacta
     7801 caacagcaat tcgggcggta aatacgccga atactacacg ccgcatgcag tggcgcggat
     7861 tatggcggat attttggtgc cggaagatgt gcgcgggcaa atccgcagcg tggacgttta
     7921 cgacccgtcg gccggttcgg gaacgctgtt gatgaacgtc gcccatgcca taggcgaaga
     7981 caaatgtatg atttatacgc aggatatttc gcagaaatcg tccaatctgc tgcggctgaa
     8041 tctgattttg aataatttgg tgcattcttt gaataatgtg gttcagggca atacgatttt
     8101 gtcgcccgcc cacaaagacg cttcaggctg tctgaaaaaa tttgatttca ttgtttccaa
     8161 tccgccgttt aaattggatt tcagcgattt ccgcgatcgg ctggaaagcg atgaaaacca
     8221 cgaacgcttt tttgctggta tcccgaaaat caagccaaca aagaaagaga agatggaaat
     8281 ctatcagctt tttatccagc atattctgtt cagcctgaaa gaaaacggca aggcagcgat
     8341 tgtattaccg accggcttta tcacggcaaa atcgggcatc gataaaaaaa ttcgcgaata
     8401 tctggtggaa aacaaaatgc tcgccggcgt ggtgtctatg ccttccaata ttttcgctac
     8461 gacgggaacc aatgtttcta tcttgtttat tgataaagtc aataaggata aagttgtatt
     8521 aatcgatgct tccggtttag gtgaaaaaat tagtattaat gacaaccaaa aaactgtact
     8581 ttcccacgaa gaagaacaaa aaatctgcca taccttcacg cacaaacagg cagtagaaga
     8641 tttcagcgtg gtggtcggct atgatgaaat caaagcgaag aattacagct tgtcggcagg
     8701 tcaatatttt gaagtaaaaa ttgattatgt ggatatttcc gccgaggaat tcgagcagaa
     8761 aatggcagaa ttttcggcag atttggacaa gctctttgcg gaatctgccg aattggaaaa
     8821 agagattaag gatagattgg cgatgttgaa attcaattca taaataccga tgaatgaaac
     8881 cggtcaaact taaaatgccg tctgaaagcc tttcagacgg cattttgttt ggtttggaaa
     8941 atacttatgc cgttctttcc ctgaaagaga gaatccaaaa aaccaaagcc acaggaattt
     9001 atcggaaatg acaaaaaccc gacgaaccgg attcccgcct gcgcgggaat gacgaattag
     9061 aagttacccg aaatttgaaa aaacaaaatc caacccaaca gaccgggttt tcgtttgcac
     9121 ggaaataatg caataaataa agaaaatata aagtatttga atttactata ttatttttca
     9181 gcttcttcaa agccgacgac ttcgaggccg aagccgatga ggccggtgaa ggatgacggc
     9241 tggccgagga cgcgcagttt tttgacgttg aggccggcga ggatttgtgc gcctatgccg
     9301 tagcttttgc tgtcccattt gtaggcttgg tttgcgcctt tgggcagggt gcggtcgagc
     9361 agggatgcgc cgtcttcggc gcggtgcagg aggatgacta cgccgctttc ggcttgttgg
     9421 atgcgctcaa gggctttagg caatgaccag gaatggcgcg ggttggcttg gatgaagtcc
     9481 atcacgctga agggttcgtg gacgcggacg agggtttctt cgtcggcggt tggtgtgcct
     9541 ttgacgaggg cgaggtgggt ttcgccggag agtttgtcga cgtaaacgtg ttgttggaac
     9601 tcgccccacg gggtttgtac gggtgcgttg cccatgtctt cgagcaggct ttcggtgcgg
     9661 ctgcggtatt cgatgaggtc ggtaatggtg ccgattttga ggttgtgttc ttcggcgaac
     9721 ttcatcagtt cgggcatacg cgccatggtg ccgtcgtcgt tgatgatttc gcagatgacg
     9781 gcggcgggaa tcaggccgtt catttgtgcc aggtcgacgc cggcttctgt gtgtccggcg
     9841 cggacgagta cgccgccttt ttgggcgcgc agtgggaaga tgtggccggg ttggacgatg
     9901 tcttctggtt ttgcggatgg tgaaacggcg gtttgaatgg tcagggcgcg gtcggcggcg
     9961 gaaatgccgg tggtaatgcc gtgggcggct tcgatggaga cggtaaagtt ggtgccgtat
    10021 tgcgcgccgt ttttttgggt catcatcggc agcccgagtt tttcgaccat ttcgccgtcc
    10081 atcggcaggc agaccaagcc gcgcgcgtgt ttgatcatga agttgatggc ttcgggggta
    10141 acgaattgcg ccgccatcag caggtcgcct tcgttttcgc ggtcttcggc atcggtgatg
    10201 atgaccattt tgccggcttt gatgtcggct aggatttcgg ggatggggga aatatgggac
    10261 atggtgtgtt ttcctgtgtg ttcgggcggg cgggcggtgt gttcggtatc gagccaaaag
    10321 gcgggcattt ttgcagcctg ttcgatttta cgggcttttt tctcgccgaa ggatttgttt
    10381 tttaaaaggg cggagagttc gcccgtattg aggcagacgg cttcggcaaa acgggtttgc
    10441 aaaccgccgt aatgtttttc tatccactgc cgcaggttgc ggcggcgcag gtcggctgtg
    10501 tcagtcattg tttttccctt tgcggaaacc gaatgtagcg gattgtaatc tcgtatttac
    10561 caaaaagcaa atctgttttt tcgccaaacc gaattatttg ctttttggta aacagatgcc
    10621 gtctgaagcc ggattcgtgg cttcagacgg cattgccgca atcaccaccg gcgaggcggc
    10681 tgtttcttcc cgcccgcttc cgattcgggg tttgccggaa gcttattccg aaaaaaattt
    10741 ctgataaacg gccttgattg ccgggtacag cataggcggt acgagcaggc gcaatatcgg
    10801 caatcctatg atgaatgcgg tcttcccgat aaaaaccgga cggattgcct tcgcgccgaa
    10861 tatctttctg catttttctt taaatgaaaa atatttccgg tagatgagga taatgttttt
    10921 ggaaaaataa agccttacca atatagacct cagatagccg atattgtcga agacaaattt
    10981 ttgcaaaccg ccgtagatgg gggaattttt gttttcgact aaaaaatcca caccccggtc
    11041 gagatgcgtc agcacatcgc tgaaattttt gtatttgatg ttgtggctga tagagccgcc
    11101 gagaacgcgg tactggtaaa aagggttatc gtaaaaggca aaagtcttga taaaacgcgc
    11161 caattgcaaa ctgtacggga aatcctcgtg aatgtatcct tttgggaaaa acagattgtt
    11221 tttgatgatt atttcccgcc tgacgatttt tgtccacgcg ttggcgatat agtaccgccc
    11281 ctccaccaac gtttcaaaat ggcggacaaa atcattatcc gaaaagtccg cccctttggg
    11341 gatgtcgcgg taattgaagg acgagggatg caggatcaaa tcaacctttt tgtctgcaag
    11401 ctgttgtaaa tcaaagagaa tccccccccg cgttttttga acggttggta tcggcccaat
    11461 agtcgtcgct gtccaaaaag atcaggtaat cgccttttgc cgctcggata ccggcgttgc
    11521 gggcatccga cagcccgccg ttttcttgat gaatcacttt tatatgcgga tatttgcctg
    11581 catattcgtc gcaaatcttc ccgcagccat ccggcgaacc gtcatcgacc aaaatcattt
    11641 cataatcggc aaaattttcg gcaagcacgg aatccacgca gcagcggagg tatttttcca
    11701 cattgtaaat agggacgatg atggagaaaa tcataaatat caatccatta tattaagatg
    11761 tttgcgcgta tgcctcaaac ccgcacccgc aatgcgtttg catccgcacc ctgtaacttt
    11821 ataaaactta ttgctttatc aaggtatgga aacctgtttc ccgaaaggcg gcgcaggatg
    11881 cccgttccct gcatctttgc cttattccga catcagatgc cccagcttgt ctgctttggt
    11941 ttggaggtag cgtttgtttt caaggttttc cccgacgtgc agggggatgc gttcgaccac
    12001 gttaatcccc gcatctttca gggtttggat tttttcgggg ttgttggtca gcagtttgac
    12061 cgagcggatg cccagatatt cgtagataga ttgcgccaaa cggaaatcgc gggcatcgac
    12121 gggcagcccg agtgccaaat tggcttcaac cgtatccata ccttggtctt gcagatgata
    12181 ggcgcggatt ttgttaatca gcccgatgcc gcgtccttcc tgacgcagat agacgatgat
    12241 gccgcgcccc tctgtctgta ccgccctcat ggccgcttca agttgaggtc cgcagtcgca
    12301 ttttctcgag aacagcgcat cgcccgtcag gcattcggag tggatgcgcg tcagaaccgg
    12361 attgccgtct gaaaaatcac cgacggtcag cgcgacgtgt tcctgcccgt ttgcctcttc
    12421 aaagccgtgc atcgtaaata cgccccattc ggtcggcagg cggcaggaag caacatagtt
    12481 taataattca gacattttca tctccttctg cggcagtgat gcccaaaagc tgcttcaaag
    12541 gttcggacaa agccaaggca agagccaccc attccgcctg cgctgcctca tccgcacact
    12601 ctgcgcattc aaattccaca tgaaccacgc ccaacacacc gccactttcc gtgcagaccg
    12661 gaatggaaat ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctcc cccaaatcca
    12721 accaacggcg tacatccgag gcaacattca tccaaccgct ttgcgccgaa cggcaagcca
    12781 gcgaaacctt gcctgccgct tcatccaaat cccacaggtt ttccaaaacc tcgccctgtc
    12841 gggacaggca tatcagttgg aaagcctggt tttcagatgg catcaaggca tagaccgtcg
    12901 cactccgcac gcctgtcgag cgcgaaaaca ccgaatccaa agccatgaaa agccgtttca
    12961 gtgcatcttc gcggacattg tcgcacgaca ggtaatcggc aagtttccaa ccctcatcac
    13021 tgcgccacaa aaccgaacgg tctatcgaag ccgtccccat atccattacc gtctgcgccg
    13081 tcagatacgc cgcccgaacc tcgtcaagcg gcagcttcaa accctgagtc agcagaaaat
    13141 ccttaatcaa agcagcaggc atacccaaat tccgtcaata aaaacaaaaa accgcccgat
    13201 tccgatgtca ggcggacgaa aaacagattt taccgcccca aaccccaagc catcaacagc
    13261 cccgccgaaa accttgcaca aaaaaacaaa atctgtataa tcgtccaatt cccatgcgga
    13321 cgtggcgaaa ttggtagacg caccagattt aggttctggc gccgagaggt gtgagagttc
    13381 gagtctctcc gtccgcacca ataatgataa ataaattata tagttaatgc ttatcgggac
    13441 aaattagtgc cacaaaacat aagtcgtctg cttttcagcg ggcggctttt ttatctgcct
    13501 atatgtttca acacgcagaa cggtacataa agtaccgcca tttgttctac cccgaatcag
    13561 aagcgggtgc ttccgcccgc aatcagattc accgccccta atgccggggt ttcaaccgga
    13621 aagaagaaaa tattgatttt gatgatggca tcaaaaaacc ccttccgctc gtctgccaaa
    13681 aggaatggtt aagaaaagca atgcgtcttt tgatggaacg cgatatataa ggaattgagg
    13741 gaaagggcag gcgggtttct gcccgttaat cctgcggacg gtcccgccaa acccgacacg
    13801 cgcgggcata tggcggactg tttacaatat tgtctaaaaa tctgaacctg caaacgtgaa
    13861 ataaggtaga atacgccccg ttattttacc gtccaatccc catttaccaa ggaaaaacga
    13921 tgagtacttc attgagttac cgcgatgcag gtgtcgatat cgacgcaggc gaccaactgg
    13981 tcgaaaacat caaaccgttt gccaaacgca cgatgcgccc ggaagtattg ggggatttgg
    14041 gcggttttgg cgcattggtc gaaatcggca agaaatatca aaatcccgta ttggtcagcg
    14101 gtacggacgg cgtgggtacc aagctcaagc ttgcctttga ttgggataaa catgatacgg
    14161 tcggcatcga ccttgttgca atgagtgtca acgatatttt ggttcaaggg gctgagccct
    14221 tgtttttctt ggactatttt gcctgcggta aattggatgt tccgcgcgcg accgatgtta
    14281 ttaaaggcat tgcccaaggt tgcgaagaat ccggttgcgc cctgattggc ggggaaactg
    14341 ccgaaatgcc gggcatgtat cccgtcggag aatacgattt ggcgggtttt gccgtcggcg
    14401 tagtggaaaa agagaatgtc attaccggcc gcagtatcgg cgcgggcgat gtggtattgg
    14461 gtttggcttc caacggcgca cattcaaacg gctattccct tatccgtaaa atcatcgaac
    14521 gcgacaatcc cgatttggat gccgagtttg ataatggcaa aaccttgcgt gaggctgtta
    14581 ttgcgccgac ccgtctgtat gtgaaaccga ttcttgccgc tttggaaaaa tttaccatta
    14641 aaggtatggc acacattacc ggcggcggca ttaccgaaaa cgtgccgcgc gtgttgcctg
    14701 aaaacacggt tgcacaaatc gatgctaaat cgtgggaatt gcccaagctc ttccaatggc
    14761 ttcaaaaggc gggcaatgtg gaaacccaag aaatgtaccg aacctttaac tgcggcatcg
    14821 gcatggtcgt tattgttgct gccgaagatg ccgatgcggt tcagggtctc ttgggtgaac
    14881 aaggtgaaac agtttaccgt ttaggtttga tccgtgagcg tcagggagac gagcatcaaa
    14941 cccaggttgc ctgattgctt ctatagcgaa atgccgtctc aaacatgggt ttggacggca
    15001 tttttatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata
    15061 gtacggcaag gcgaggcaac gccgtactgg tttttgttaa tccactatat attgattgat
    15121 atgagggtgg gttacacatg cttaaacgat ataagccatt gtatatttat gggattcgga
    15181 atggaacatt ttaccctgca ttagtatttc cgctttttat tatgccgtct gaaaatatca
    15241 tgatgatgcg ttttcagacg gcatagctga tattgcagta tcagataggg gcaaacggaa
    15301 aacactgaac cgaacaatat tactgctatg ccaagcgtac ttatgggggc ataaacttac
    15361 atgcttggtc tgttatccga atgaccgtac ggggattaaa acccctaata tgccgtctga
    15421 acccgcctta ttccagcacg ggttggggga cgttgtgccg gatggcgtaa acagcggcct
    15481 gcacccggct gctgaggttg agtttgcgga gcaggttttg aacgtggact ttgacggtgg
    15541 attcggcaag atcgaggtgg cgggcgatga ttttgttgct gtgtcctgcg gcgagatagc
    15601 ccaagatttc cagttcgcga ggggtaagtg aggagagtgc ctgcgtccct tgggcaggtt
    15661 ggggggaaat caggcttttg acgagtttgg cggtcatctc gggcgagaat acattatcgc
    15721 cttcggcggc tttgcgtatg ctttcgagca gaaagtcggc gttgatgttt ttcagcaggt
    15781 agccgcgcgc gccgatgcgc atacattcgg tcaagtcgtc gttgtcttcg gaaacggtca
    15841 gcataatcac tgcctgctgc ggattgatgc tgatgatttg ggagagtgct tcgcgtccgt
    15901 tcataccggg catatcaagg tcaagcagga cgacatcggg ttggagccgg ttgatcattt
    15961 taatgcccga gaggccgtct gcggcttcgc cgatgacctc aaaatcatgt tggcgcgaca
    16021 aaagggattt aatgccgctg cggaagaggg tatggtcgtc tatcaggata attttaatgg
    16081 tcatttcaag ctttcttcag atgcaaccgt caatgagacg gtggttccct gttgagcttg
    16141 ggaacggatt tctaaaacgg catggatgcg tttggcacgc tcctgcatga tgtgcagtcc
    16201 gacatggctg cccgtgggtt ctcctatttt ctccgtgtcg aaaccttgtc cgttgtcttg
    16261 gatggtcatg gtaaagcgtc cgccgtgttc ggaaagggtg aattttacat gggtggcgcg
    16321 ggcgtgtttg cggatgttgg acaggctttc ctgcaggata aaaatcattt ggagctgcgc
    16381 ttcctgaggc ggcaggaacg aaccgttttc ccaggcggtt tcgaccgtta tcccggtttg
    16441 ttgcgtaaag cgggcgaata ggtcggcaac ggcttcggga aattctttat tgctgatttt
    16501 ggtacggaag ttgagcagca gttcgcggac atcttcataa cattcctgca cgcctgtttt
    16561 gatgaagccg atgttttctg cggcttcctc ccgtttgttt tcggcaaagg cggtttccag
    16621 catctgtacc tgtaggttta ggaacgttaa tgcttgtgcg atgctgtcat gtaatccttg
    16681 cgcaatcagg ttgcgttcct gcaatactgc aagcaggcgt ttttcttcct cctgttttgc
    16741 gccggcaagc gatacgccca attgcctgcc tagtgtttga agcaggatgc ggtcgtcttc
    16801 atcaagagaa atgccgtttg gaaagctgag caacagcctg cccaatgttt cgttctggta
    16861 ctcaatgggg aagatttcct catggtactt ccccaaatcc gaagctgctg tgccgcaatc
    16921 cgcatgatga atggaaacat aaacatcgga tccgccgtcc aaacaaactc tgccggaatc
    16981 tgctcctacg gcgggcagga tacggtttag aaaatgttct gcagcctgtt gcggtatgta
    17041 ggattggtgc aggtcccgtg tagtttggta cagcagggtc aggttttgat tttgtttttc
    17101 gagactgcgt gtctgctcgg cgacttgtcc ttccaaatca tcatataaaa ttttcaacct
    17161 gccgcccatt tgattgaaac aacgcccgac ctgtttgaat tccggcgtac cgccttcggg
    17221 aaccggaata tcgaaacacc tccgtccgat gcgttccgca ccttccctta acgcctgcag
    17281 cggccggata acccaaatct ggtgccaaaa cagcatcagt acagacgaca ccagcgtcat
    17341 caacataatt gcccattgaa aacgcctgag ccaccatgtg tttttttcgt tggcattttc
    17401 caatgcctgc aaaaacagtt cgatgtttcc ggcaaagcgg tagagatcga cctgagtcgg
    17461 tcgccggtag gactggagcg gggggaggat gtgtgcctgc caatctataa tcagcatgga
    17521 ttgtatcaaa tcataagcaa gaggggtgtc cgaaggaatc agcggatgga tggcatcact
    17581 ttgtgaaata cgattcaggc ttttttcaaa ttcgtcaacc tgattgtcta tttgggagcg
    17641 gggagatcct tcacccgcca tgtatgccag acggtatgcc tgcattctca agttgcccgc
    17701 ctcttcgatg acggaggccg cgttttccag acgcaaagag agcagcagtg tcaaaacgac
    17761 agacaatgcc gccaacccga cccacactcc ggtcaggagt ttcaggcgta gggaaaggct
    17821 gatgccgtct gaaaaacggg ctggcagtat catgctcggc gaaaaattgt tctaaataat
    17881 gcaaacaata tcattctttg ggattagata caactgctca gaaagaattg gttaaggaaa
    17941 acataatccg taccgcttca gtgttaaatc atgttatgat aaaattcaga taatatgaat
    18001 ttatttgccg tctgcttcag acggcatttt gagtcattgg gaacggcaga ctgcaatcat
    18061 gaaaacctcg aacttttcca agagaacatc cgcaatgaaa acttttgccc tgatactggc
    18121 cgacggtcag gcgagccgta tgggaggcga ggacaagggg cttgctcttt tggggggtaa
    18181 ggcactgata gatcatgtca tcgacagggt caggccgcag gtcagccata tcgccatcag
    18241 caccaaccgg aatttggaag aatatgctcg aagaagtccg catatttttc tggacgcacg
    18301 gcagtggcag catttcggcc cgctttcggc attgtgtacc gcagccaacg atttgcagtt
    18361 ggcgactgcc gactggcttt tggttgtgcc gtgcgatatg ccgtatctgc cgggcgattt
    18421 ggtggcgagg tttgaaaccg tgtcgaaacg cacaccgttg tgcaatgcgt attatgtgga
    18481 aacgccgata acgatgcact acaacattat gtatatccgc ccgcagattc tgcaaagcgc
    18541 gattccctat ctgttttcgg gtatgaaaac attaagaagc tggttgcagc agcaaagggc
    18601 gcggccggtc agattcgagt ttgacgggca ttttgccgac ttgaacacgc aaatcgattt
    18661 gcaggaggga taagggcaag accgccgacc ggcgtggaaa ggaaaggtca agccataccg
    18721 ggcggttttt gcccgaatcg gaggcataat gctgtctgaa ggcatttcag acggcatttt
    18781 tcgcggggag atgctacaat ttgcaccatt tttaccgaca cagggaaaca ggattatgtt
    18841 tacaggcatt gttcaaggat tgggaaaact gacggcaatc caccgcccgt cggaggcatt
    18901 tcacacttat gtcgtcgagc ttccgcaaga ggcggcggac aatctgcaac gcggcgcatc
    18961 ggtcgccaat aacggctgct gcctgacgat taccgaaatc gaaggaaacc gcgtcagttt
    19021 cgatttaatg gcggaaactt tggcaaaaac caatttgggg ctgctgaagg aaggcgattg
    19081 cgtcaacatc gaacgggcgg cgcgtttcgg agacgaaatc ggcggacacg tcatgagcgg
    19141 acacattatg gcaaccgtgc ctattgtcga aatcgaacgg gacgggttca accgcacggt
    19201 ttggttttcg ctcccgcatg aactcaaacc ctatatcctg accaaagggt tcgtcggctt
    19261 ggacggttgc agcctgacca taggcaaagt cgaagacggc cgtttcaatg tccatctgat
    19321 tcccgaaact ttggaacgga cgctgttcgg cagcagaaag gtcggcgaca ggatcaacat
    19381 cgaaatcgat ccgaatacgc aggcaatcgt cgataccgtc gaacggctga tggcgcaaag
    19441 atatgcaaag tgaggcagag atggaattga acgaatttct cgataaagcc tatgccgttt
    19501 tgcggcgttt ggatgccgtg cttccgcccg aacccgggca tacggattgg aacgcgcttg
    19561 ccttccgctg gcagagtgcg ggcaaaaaag gttttttgga acacttgccc gatccgcaca
    19621 cctttccctt ggtaaggctg gcgggagtcg gcaggcaaac cgaattgctg gtgcgtaata
    19681 ccgaacagtt cattgtcggc agacccgcga acaatgtatt gatgagcggc gcgcgcggaa
    19741 caggcaaatc ctcgctggtc aaagccctgc tacacgaata tgcggataag ggattgcgcc
    19801 tgatcgaagt cgataaaagc gatttaatcg gcctgcctta cctgttgacg cttttgaagg
    19861 aacgtccgga aaaatttatc gtattttgcg acgatttgtc gtttgaaagc ggcgatgaaa
    19921 cctataaggc attgaaaacc gcgttagacg gcggtctgtc tcaacgttgc gccaacgttt
    19981 tggtttacgc gacttccaac agacggcacc tgatgcccga atattttgac gagaatgccg
    20041 gtacgacggg gatgcggggg gaaatccatc agaaagaagc ggtggaagaa aaaatatccc
    20101 tgtccgaccg attcggattg tggctcagtt tttatccgtt cgatcaaaac gattatctgg
    20161 cggcggtgca aaattggctg gaagatttcg gcgtgccgta tgatgaaacc gcacagatgg
    20221 cggcattgca gtgggcgcag acacggggca gccgttcggg acgttccgcg tggcagtttg
    20281 cctgcgactg ggcgggcagg ctgccgaaac aacgggcgtt gtgagcgttt cggggattat
    20341 ttgaggcagg aagaaagcca catttgctgc tgttgcggtg tgaggatatg gaagaaccgg
    20401 tgttggattt ccaattcgtc caccgcaaaa tccataccgg acaaatagcg gctttcgaca
    20461 taatcgcgcg cctcgttccg attaaaaaca tccgaggaaa tgatttcgac gacagaccgg
    20521 cggcggctgt gttcggaatg cataaccttc aaacgcgccc tgtcgcccgc cattttgaag
    20581 gcggtgcgga ttttacgcag ctcattgtgc tgactttggg tcagcccgag tcggcgtatg
    20641 tcgcagttcg gttgaaaatc gttcgggggg atataagccg tggcggcgtg ggaaagctga
    20701 caggaaagca aagccatact taaaaaagag gcggcaggtt tggcaaaacg gcagggagca
    20761 ggcagtggca cggcttttcc tcttgttgga aattttagga aatatactag attgacgtgt
    20821 gaaagatggt attatcgcgg ttaacttttg ttaataatgc gtacgtatgt ccggcaggca
    20881 gacataacgg cggaatcgga a
//
LOCUS       DAWFJW010000024        20548 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFJW010000024 DAWFJW010000000
VERSION     DAWFJW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.024"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..171)
                     /locus_tag="WGR19_000735"
     CDS             complement(<1..171)
                     /locus_tag="WGR19_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0798297.1"
                     /translation="MSTFFRQTAQAMIAKHIDCFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPL"
     repeat_region   611..2362
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=677..712
                     /rpt_unit_seq="gttgtagctcccattctcatttcgcagtgctacaat"
     gene            complement(2426..2752)
                     /gene="cas2"
                     /locus_tag="WGR19_000736"
     CDS             complement(2426..2752)
                     /gene="cas2"
                     /locus_tag="WGR19_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0798298.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(2745..3659)
                     /gene="cas1"
                     /locus_tag="WGR19_000737"
     CDS             complement(2745..3659)
                     /gene="cas1"
                     /locus_tag="WGR19_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ0798299.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNDESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLVVIHLYEQGRLKTELTPSIKQHLIKTLHCQTSIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKAYQYE"
     gene            complement(3725..6970)
                     /gene="cas9"
                     /locus_tag="WGR19_000738"
     CDS             complement(3725..6970)
                     /gene="cas9"
                     /locus_tag="WGR19_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0798300.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKECGHIRNQRGDYSHTFSRKDLQAELNLLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPTEPKAAKNTYTAER
                     FVWLTKLNNLRILEQGSEQPLTDTQRAKLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYNEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFQEYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVAYADEEDWTVIDESFRFKFVLYSNDLIKVQLKKDSFLGYFSGLDRATGAI
                     SLREHDLEKSKGKDGMHRIGVKTALSFQKYQIDEMGKEIRPCRLKKRPPVR"
     gene            complement(7248..10682)
                     /gene="dnaE"
                     /locus_tag="WGR19_000739"
     CDS             complement(7248..10682)
                     /gene="dnaE"
                     /locus_tag="WGR19_000739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0798301.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLVAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     NFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            11134..11448
                     /locus_tag="WGR19_000740"
     CDS             11134..11448
                     /locus_tag="WGR19_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0798302.1"
                     /translation="MCCLFCGNFGYGGGRCYNNGLFLQGNIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EA"
     gene            11426..11635
                     /locus_tag="WGR19_000741"
     CDS             11426..11635
                     /locus_tag="WGR19_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798303.1"
                     /translation="MDTTLKHKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            complement(11677..11919)
                     /locus_tag="WGR19_000742"
     CDS             complement(11677..11919)
                     /locus_tag="WGR19_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798304.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNKCMANAKEKPLCRHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            11928..12764
                     /locus_tag="WGR19_000743"
     CDS             11928..12764
                     /locus_tag="WGR19_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0798305.1"
                     /translation="MIDLHCHSTVSDGMLSPAEIVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIADKLEKKGIGGAYDGALELAANKEMVSRTHVAEFLIKNGYVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            complement(12817..14109)
                     /locus_tag="WGR19_000744"
     CDS             complement(12817..14109)
                     /locus_tag="WGR19_000744"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0798306.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(14167..16077)
                     /gene="pglD"
                     /locus_tag="WGR19_000745"
     CDS             complement(14167..16077)
                     /gene="pglD"
                     /locus_tag="WGR19_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0798307.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRSACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(16125..17300)
                     /locus_tag="WGR19_000746"
     CDS             complement(16125..17300)
                     /locus_tag="WGR19_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0798308.1"
                     /translation="MLNTALSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPNTKAIIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKALWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            complement(17419..17529)
                     /locus_tag="WGR19_000747"
                     /pseudo
     CDS             complement(17419..17529)
                     /locus_tag="WGR19_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17529..17723)
                     /locus_tag="WGR19_000748"
     CDS             complement(17529..17723)
                     /locus_tag="WGR19_000748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798309.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAATAQKPQDRDGISD"
     gene            complement(17771..18457)
                     /locus_tag="WGR19_000749"
     CDS             complement(17771..18457)
                     /locus_tag="WGR19_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0798310.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDSKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            complement(18444..20021)
                     /locus_tag="WGR19_000750"
     CDS             complement(18444..20021)
                     /locus_tag="WGR19_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0798311.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCI
                     TVQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            complement(20014..>20548)
                     /locus_tag="WGR19_000751"
     CDS             complement(20014..>20548)
                     /locus_tag="WGR19_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0798312.1"
                     /translation="RSKTVRLFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGG
                     PDLEKLKQYENRAIKFYGYLPYSEMMSIAKACDIAVNAIHSHAMQSVTNKLSDYMALQ
                     KPILNSQTNAEVLGLLNLLPHENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFKR
                     DVAYQKIVNLIERLANE"
BASE COUNT         4403 a         5016 c         5659 g         5470 t
ORIGIN      
        1 cagcgggtag gcgggacggc cgcggtggtc tctaaggtaa cgggtttttt gacggttcag
       61 gtattgttcg attggttgcc aatcaatcac ctggtccaac ttcaatagtg ggaagcagtc
      121 gatgtgcttg gcaatcatgg cttgggcggt ttgccggaag aaggtgctca tgagaaatcc
      181 cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg tctcattttg
      241 caagatttta gtttaatcat caaacaggga acagcaaaaa tccccgctgc aacggggatt
      301 tttgttcggc tctcgcctgt gtgattaact tcactaggag aagctaaaat gcttaaattc
      361 cttatgcgtg ggaaaaagtt gctgctgtcc gttgaagtga accacaagtt cattttagcg
      421 gttgctttgc tgttaagtca atgcagtagc taacccgacc acaatcaaaa aaacgcaagt
      481 tttccgccta ctctgtgagt gggcggtttt ttcttatttc aaatctgtga cacaatatct
      541 gtcgcctttc ttgccataat tgcactgtgt ctcaatgact ttaacgcacg ttcgcttatc
      601 gcaacggctg gttgtagcgc ccattctcat ttcgcagtgc tacaatgttt cttcccatat
      661 actgctgttc aaaccggttg tagctcccat tctcatttcg cagtgctaca ataatttaat
      721 acggcgacca tctcgtgcca aggttgtagc tcccattctc atttcgcagt gctacaataa
      781 tttaatacgg cgaccatctc gtgccaaggt tgtagctccc attctcattt cgcagtgcta
      841 caattcatca atcgcccact caaacccaag catagttgta gctcccattc tcatttcgca
      901 gtgctacaat gcctcacaaa tatcgtcaat gcttgcgcga gttgtagctc ccattctcat
      961 ttcgcagtgc tacaattgcc acttgatgtt ttagccatag tgttctgttg tagctcccat
     1021 tctcatttcg cagtgctaca attgcgaagc gaacaccctg aacaatgccg acgttgtagc
     1081 tcccattctc atttcgcagt gctacaataa atgtgttttt gcccatgatt cagctacggt
     1141 tgtagctccc attctcattt cgcagtgcta caatcatgcc atgcatcaat gccaaagttg
     1201 tccggttgta gctcccattc tcatttcgca gtgctacaat ttgaaagccg cttgtatcaa
     1261 tgatttcaag gttgtagctc ccattctcat ttcgcagtgc tacaataacc gccaccacgg
     1321 catcaccgcc aacgaagttg tagctcccat tctcatttcg cagtgctaca ataggtcaag
     1381 ccgcttcaac agagttttat tggttgtagc tcccattctc atttcgcagt gctacaatcg
     1441 taaaagctca aaaacatttg caggtcatgt tgtagctccc attctcattt cgcagtgcta
     1501 caatataaca gcatagtgag ccgctacgcg gctggttgta gctcccattc tcatttcgca
     1561 gtgctacaat tagccgccgc catagtggag gccgcttgtt gttgtagctc ccattctcat
     1621 ttcgcagtgc tacaatgagt tcgaaaagtt cctgcgcgat gcaggggttg tagctcccat
     1681 tctcatttcg cagtgctaca attcatcgcc cctgctttca cattagcaca aggttgtagc
     1741 tcccattctc atttcgcagt gctacaatcc ttaaatgtga gtttttcatg tttgaggcgt
     1801 tgtagctccc attctcattt cgcagtgcta caattttaat cgtttgatgg cggcgggtga
     1861 tttggttgta gctcccattc tcatttcgca gtgctacaat atttttggaa atagaaataa
     1921 atcagggtat gttgtagctc ccattctcat ttcgcagtgc tacaattcat ttcgttgatt
     1981 ttaatatcca tctcaagttg tagctcccat tctcatttcg cagtgctaca atcgcgccct
     2041 aaaaacagcg cgctggtttt ctgttgtagc tcccattctc atttcgcagt gctacaatcg
     2101 cgccctaaaa acagcgcgct ggttttctgt tgtagctccc attctcattt cgcagtgcta
     2161 caatttcact tctgtaacga tagcattaac aacagttgta gctcccattc tcatttcgca
     2221 gtgctacaat attaaataag gatttaatta tgaaatttat gttgtagctc ccattctcat
     2281 ttcgcagtgc tacaataaat ccctcaccag ccaaagacgg tttgccgttg tagctcccat
     2341 tctcatttcg cagtgctaca ataccccaac gggaaatcct tattctataa ggatttcccg
     2401 ttttatttgg tctgaaaaaa tgggcttaaa ataacaataa ttggtctgaa ttgacctttt
     2461 tttcttgggt tttcagctcg cccaacagca gtttcatggc ggcatattgc ttttctgtta
     2521 tctccaaaca gcggattgag ccttcttgcg gaaggtttgc acatagtcta ttgtggtgtt
     2581 tttgcaacga atcgcggcct ttgacgatac ggctgtatac ggaaagttgc agcatttggt
     2641 atccgtcttt taataaaaac tggcggaatt gattggcggc tttgcgcttt gccgccgtga
     2701 taaccggtag gtcgaagaag acgataatcc tcataaattt ggcctcactc atattggtat
     2761 gctttcaatg gtaggatttc aggcagtttc agttgtttgg cgtttttatc agttacgccg
     2821 gcttgaaatg acgaaaccat tttgtcgatg gcagccaagg tgctgaaatg ttgccgttcg
     2881 atgctggttt gacagtgaag ggttttgata aggtgttgtt tgatgctggg cgtcagttca
     2941 gttttcagac ggccttgttc gtacaaatgt ataacgacca gatcggccaa tgggcgtaag
     3001 ggttcgataa agtcatcggc gagattgaat ggattcaact cacttctatg aaacaagcct
     3061 aatgcaggca accaaccgta taaggtcagg gcgcgtgcaa ctgcagcccg gagcacagcg
     3121 taggtatagt tcaatgcggc attgacggcg ttgttgtcat tgcgggtaaa tttttcgcca
     3181 aagagggctt ggaagtagag ggcggcggct tgggcttcgc ggtttcctgt atcgcccgag
     3241 cggacttcag atgccaaagt gcgcagccgt ttggctgcaa gatcattgcc ggtttcgtct
     3301 gcgacaaatg cttgattcag gattttttgg cggacgatat gttgccagaa ttgctttttt
     3361 aagggttcgc ttatattcag ctgcagtttg aggattttaa gttggcggtg atattgggca
     3421 taaggcagcc attgtccgca gggcaggaac tgctcgtcac atgtcagtaa ggttgcgcca
     3481 tgttcggcta aggctgacag aagtggagcg gtaatcaggg tttcgcggtt ttcgatgatg
     3541 ataacggcga tgtcttctaa tggtacggtg tgggactcgt cattttgttg aatcagcagt
     3601 tgccggcgtt gcaaggatag tttgccgccg ttttgaatga gcaggctgcg ccaagtcatg
     3661 ttttcccctt attttgatat aaaaaatccc tgcttcaggt cgtctgaaac agggatagat
     3721 taatttaacg gacaggcggg cgttttttca gacggcatgg tctgatttct ttgcccattt
     3781 cgtcgatttg gtatttttga aaggaaaggg cggttttaac gccgatacga tgcataccgt
     3841 ctttaccttt tgatttttcc aaatcatgct cccgaagaga aatggctcca gtggctcgat
     3901 ctaatcctga aaagtaacct aaaaagctgt cttttttaag ttgaacttta attaaatcat
     3961 tcgaataaag tacaaattta aatctgaagc tttcatctat tacagtccag tcctcttcat
     4021 ctgcataagc aacaacagcc ctatccggca aaatcccttt cgctacctgc caactgtaaa
     4081 tcggtaccag ataatacttg tcgcctttct caaacacatc tacgcgcacc atggttgcgt
     4141 tgtcggcaat accgttatgg ttgcgcaccc atacgccggt tttctgtact tgctctacgc
     4201 gtacggcttt tacctgttgg gtgcggttgc ctgctttatc gtatttgtaa aacggctcgg
     4261 caaaggcttt ggcaggatcg tctttatgtg cttccagccg tgctttcagt gcttcgtata
     4321 gcttaggttc gcgctcccga ttgaccattt tttccaagtc tttcagtttt aactgtgtta
     4381 acggtacgcg caacacgctg acgccttcgt ccagtcgttt ggcggatttg acggtctcca
     4441 tatgcccttg cccgctcatc ttccgattgg gcgcgcgtga aacaaacagt ggcgtaacgt
     4501 attcgtgtac ggcttcgggg cgagatgata atttttcggc aagcaacgtg cgcagttttt
     4561 ctggggtatc ggcttcttcg aattcgggtt tgccgtccgg tttgccgaag acgcgaatca
     4621 tgacttcttg tgcgaaaaat tcccaaggtt gtgggaagtg tgttttttga tgcagcactt
     4681 ctcctgtttc tttgtctatg gttttaccgt caaacgcgtt catctcttta tagcgtacaa
     4741 aacgggtaat tttctgctgc atggcaacgg tcgagcaggc aacgacgacg gcgtccaagg
     4801 cgtgatggcg gtcgttttcc gcacgcactt tgcgcaatcc ccaaaagccg cgcaacagat
     4861 tggtaatttg tccgttggat gcaaagacac gtttcttgcc tttacctgtc agccgcatac
     4921 ggtcggcaac aaattgacac aggaaacggt tgacgtagcg cgtgtcgttc agattgcgtt
     4981 ctttaaagcc gtcttcatcg aatttttgca gcagaatccg ttgtttttta ctgcgcggga
     5041 aacggctggt ttcgacacgc gctttaaatt cctgccattc gcggctgttg tctttgccgt
     5101 tgaagtattc gtaaggggtt tgattgcctt tgttttggtt ttcgctgccc aataccagta
     5161 ctttattgtt gaaactgtcg tcccatgtgc gcgagaacgg cagggcatgg tcgatttcga
     5221 catagccttt ttcgttcaga cggcctaagt tgatttcttt gcccgaatac aggcatttgc
     5281 cgtgttgttg ctcgtacagg cgcagtttca gaatatcttt tgatttgggt tcgccgacaa
     5341 aattggggaa atactcttgg aatttggcgg cggctttttc cctgtctttg cggttttctt
     5401 cttggcgttt ctcaatttct ttgcggtctt taaacgattt acctacttcc cttgcagttt
     5461 caatatggat acgagctggg gagccgtaac ggcgtaccac gccgttaatg accttacgtg
     5521 cttgagataa ggcgcgcaag acgacggggt tgcggatttc gtcggcggga atcggcggca
     5581 gataaatctt ttcttccgta ttcttcttgc cgtaatggtc tccgtagatt tcggcgcagg
     5641 cttcattgta acgtttgcct tgttccatta gaggcacaat tcggcgcaat gctttcaagg
     5701 aaatttggac gaacttatcg aagctgatgt gtttcaacag cgcttctaag atttcgggct
     5761 gaacacggtc tttcagacgg cctgtaatgt cctcatcggt tttgaacaag gagaatgccg
     5821 tgccgatttc gtcttgtaat tcgggagaaa ggtttaatgg ggattttttg tctttcaatc
     5881 cttctttttc cagcgcacgg ctgatggcat ggtaggcctt catttccatc aacgttgagg
     5941 cttcggcatt gtccttacca tagcgcaagc ctttgaaaaa ggcggtatct tctaaaccca
     6001 gtagcttacg ggcttgtgcg taagtcagtt tggattttct gtatggctcg tccataagct
     6061 tggcgcgttg ggtatcggtc aatggctgct cgctgccttg ctctaaaata cgcaggttgt
     6121 tcagcttggt cagccagacg aaacgttcgg ctgtgtaggt gtttttagcg gctttcggct
     6181 ctgtcggttc gaaggtgcaa tgtcccaaca ttttttgaac agcatcgccg gatagggcgg
     6241 ggcgttgcgt catcagcagg gtttcaatac cttctttaag gccgcctgaa acatgcggat
     6301 tgccgaattc tttttgtttt tcaaacagta aattcagctc cgcctgcaaa tctttgcggc
     6361 tgaacgtatg cgaataatcg ccgcgttggt tgcggatatg gccgcattct ttctcaaatt
     6421 tatttaaagc caattcggcc ggtgtgcgga aatcgcctgt ctgtaaggca tgggcattgt
     6481 cggctacgcc tttgagcaaa gcgccaagct ctttatcggc agtttcgccc tcgttttttc
     6541 tttgcgacaa ataaccgcgg tgtttgatta aatgcaataa gactgccgac cactctaaag
     6601 gcgtcagttt gcggtctaat gcggctgcgc gaagttgcca tggtgtattc ggtaaggatt
     6661 tgatcaagcc gttttcgtca aaatcagcgg cttgtaatac gccttcgcgt ttcaataggc
     6721 ggcgggcccg aagcaggcga tgggcgcgac ggcgggtcag gcggcggaca ctgcgcgcca
     6781 aacgccttgc catggcaagg gagtcgcctg ttttcggtac ttcggcacgc tcaaatacgc
     6841 gcacgcccaa atcaatcagg cggatggggt tttcttcttc gtcaatttct accatcgccc
     6901 agccgacgga tgcgatgccg atatcgaggc cgaggatgta gttgattgga ttaggtttga
     6961 aggcagccat ttattactcc gtaaacaacg ttgcaaataa tgataatata tcttattgtc
     7021 ttatttatat acaaatagat tattgactta tcatctcaca acggctagaa tcccaaacat
     7081 attgtcgcac tgcgaaatga gaaccgttgc tacaataagg ccgtctgaaa agatgtgccg
     7141 caacgctctg ccccttaaag cttctgcttt aaggggcatc gtttatttcg gttaaaaaat
     7201 gccgtctgaa accgattttg ggcttcagac ggcatttata ttttgggtta ccagttgacg
     7261 cgcaccgacc ggctgccgag caatgtttcc agttcgccga acaatgcgga gctcggtgta
     7321 accgtccatt tcggcggcac ttgaagcctg cccgacgctt tttcgttggc atacgacagt
     7381 tgcagcggga tgcgcggcgt gtcgggcagt tggtgggcgg cgagcagttg taccagtccg
     7441 ccgatgtcgt gatgcggggc gagggcgagg ctgaggctgc gggcgtagcg ttcgcgtgcc
     7501 gtttgcaggg tcatgacttg gtttgccaga atacgcagtc cgtcgccgcc gccgtagtcg
     7561 tcgcggctga ctttggattc gataatcagc acttggtcgg ctttgaggca gtcggcgcag
     7621 ttttccaacg tctgaccgcc gaccatgatt tcaacctgtc cgctcaaatc ttcaaggctg
     7681 acgaaggcga ttttgccgcg cttgcccatc atggtgcgta cggcggtaac gaatccggcg
     7741 aggcgcacgc tgtcttgcgg tttgagtttc gacagtttct gcggggcgat ttggcggact
     7801 tcttgggcat acgggccgaa cgggtggccg gacaggtaaa agccgatgac ggttttttct
     7861 tcggcgagtt tttccgattc gctccacatc ggcgcgtcga tgagccgcac cggttcgatg
     7921 gcgtcttcca tcatgtcgaa aagcccgccc tgattggcgt tggcggcttt ttggtcggcg
     7981 ttgtccatag cgaggtcgat gttcgccaag agcatggcgc ggttgggttc gatgctgtcg
     8041 aatgcgccgc cgcgtatcag agcttcgagg gtgcggcggt tcatgtgttc tttgccgacg
     8101 cgctcgcaga agttcaagag gccggtaaac ttgccgccgc tttgccgcgc ggcgatgatg
     8161 gattcgacgg cggcttcgcc cgtgccttta atcgcgccga gtgcgtagcg gattttcatg
     8221 ttcggatacg gcgtgaagcg gtagtcggat tcgttgatgt cgggcggcag gaactcgatg
     8281 ccgttggcgc ggcagtcgtc gtagaaatgt ttgagctggt cggtgttgtc caattcagac
     8341 gacatggtcg ccgccataaa ttcggcgggg tagtgggctt tgagccatgc ggtctggtag
     8401 gaaatcaggg cgtaggcggc ggcgtgggat ttgttgaagc cgtagccggc gaatttttcc
     8461 atgtagttga agatttcgtc ggatttttcg cgcgaaatgc cttgtttcgc cgcaccttcg
     8521 gcgaagattt ctcggtgttt caccatttct tcgggctttt tcttacccat ggcgcgacgc
     8581 agcaggtccg cgccgccgag cgagtagccg ccgataatct gcgccgcctg catcacttgt
     8641 tcctgataca ccataatgcc gtaggtcggc gcgaggatgc cttcgagcag cgggtggatg
     8701 tattggaatt cctgaccctt catgcgcgcg acgaagtcgg ggatgttgtc catcggaccg
     8761 ggtcggtaga gcgatacgaa ggcgatgagt tcttcaaact tggtcgtgtg cgccgttttc
     8821 agcatttttt tcatgccggt cgactcgaac tggaagacgg cggtggtgtt cgcatcgcgg
     8881 aagatttggt aggcggcctg gtcgtcaagc ggaatcttgc cgacatcgac aatatcgccg
     8941 gtggtgtttt tgatgttgtt ctgcgccatt tcgataatgg tcaggtttcg cagacccaaa
     9001 aagtcgaatt tcaccaagcc cacgtcttcc acgtcgcctt tgtcgtacat cgatactggc
     9061 gaggcggatt cgtctgcctg atacacgggg ctgtaatcgg aaatcttgcc cggcgcaatc
     9121 aatacgccgc ccgcgtgcat acccaagccg cgcgtcaggt cttccagctt tttcgccagc
     9181 gtaatcagtt cgtccgcttc ttccgcttcg attaattcct gaatctgcgg ctccgccttc
     9241 atcgcgtcgt ccaaacccag aggtttgttg gcttccaacg gaatcagctt ggacagtttg
     9301 tcgcacagca taaacggcag ctccaacacg cgccccacgt cgcggatgac cgctttggac
     9361 gacatcgtgc cgaaggtaac aatctggctg accgcctgag cgccgtattt atcgcgcaca
     9421 tattcgatca cgcggccgcg gtttgcctga cagaagtcaa cgtcgaagtc gggcatggag
     9481 acgcgttcgg ggtttaagaa acgttcaaac agcagcgcgt atttcagcgg gtcgaggtcg
     9541 gtaattttca gcgaatacgc caccagcgaa cccgcgcccg aaccgcgtcc cggcccgacc
     9601 ggacagccgt gcgttttcgc ccagttgata aagtcttgta cgataaggaa atagccgggg
     9661 aatttcatct ggatgatgat gttcagctca aaatccaaac gttcctgata ttccggcatt
     9721 tttgccgcac gctccgcctc gtcgggataa agctgaacca tgcgttcctg caagcccttg
     9781 tttgagagtt tgatgagata gtcatcgcgc gataaaccgt cgggcgtggg gaaaaggggc
     9841 aggaagtttt tgcccaatgt gatgtgcaga ttgcagcgtt tggcaatttc taccgtgttt
     9901 tccaaggcct cgggcaaatc ggcgaaacgc tccagcatcg tttccggcgg aatgaaaaac
     9961 tggcccggtg tgaaatcgcg cggacgtttt ttgtccgtca gcacccagcc gcccgcaata
    10021 cacacccgtg cctcgtgcgc gttgaaatca tcgctgctca taaactgcgt cgggtgggtc
    10081 gccaccaccg gcaaacccag ttcctccgcc agcttcacgc tgccggaaac gcaagcctcc
    10141 cattcggggc gttcgggcag gcgttgcagc tctaaataaa acgcatcggg aaaccatgcc
    10201 gcatatttca acgccgccgc acgcgccgcg tcttcattgc cgttcaacag attcacgccc
    10261 acttcgccgt aatgtgcgcc gctcaaacaa atcaagccgc tgttgtcgcc gttttccagc
    10321 cattcgggat tgagttccgc atgatggata ttgcggtctt tgccgacata agccgccgtc
    10381 agaagctcgc tcaagcgcag atagcccgca tcgttgcgga taatcagcat ggcgcgaaac
    10441 ggcttgtcgg gcgcattcgg attgcctatc cacacgtccg ccgcgccgat gggtttaatc
    10501 cctgcgccgc ggcaggcttt atagaatttc accaagccga attcgttcat caaatcgctg
    10561 atgcccaaag caggcaaacc gtattcctgc gctttggcaa ccagtttttt aatccgcacc
    10621 ataccgtcgg taattgagaa ctcggtatgc aggcgcaagg ggatgtaggt cggctcggtc
    10681 atggcaaaat cggcgtgaac aataaaaggc gtattgtagc agggttgtct ttagatggcg
    10741 gtgtaggtaa tgctgtttcg gattcatacg gcatgacctg caaatgtttt tgagctttta
    10801 cgacggcaaa aaaatgcctc ctaccgtgtg gtggaggctt cccaaggagt attgatagat
    10861 ataaagaact atcaaactag ttataaataa ctatatacct tattcggacg gatggcaagc
    10921 agttaaatta attttacgtt caaacaggtt tttgatttcg ttttgatgcc gatgaccagt
    10981 gtatcgggca gttcgtgttt gaggatgtgc atcagcgtca atgcggattc gtcggggaac
    11041 aggatttgca gcctgctgtt gggcaggatt tctttgatcg gatgacggca tatggggttt
    11101 cagacggcat ttttacggaa caggcggggc gggatgtgct gcctgttttg cggtaatttt
    11161 gggtacggcg gcggcaggtg ctacaataac ggcctctttc tgcaagggaa cattatggaa
    11221 gccaccgtct atctcgaaga caacgaatac atcgccctgt gcgacctctt gaaattggcc
    11281 ggacttgccg aaagcggcgg acaggcaaaa gcatttatcg ccgaagggct ggtgttgcgc
    11341 aacggcgaaa ccgaaacccg taaaaccgcc aaaatacgcg gcggcgaagt catcgagttt
    11401 gacggcgcgc gcttggaaat cgccgatgga tacgaccctg aagcataaag ccgaagccct
    11461 tttgggcgag ccgcttttag aagaacccgt ccgccctgag ttgtgggaat gctgcggcag
    11521 cgactgcggc gaggcgtgca ttcagacgat ttactgggcg gataaggcac gctacgacgc
    11581 gcaacggcgg aagttgaaag aagcgggttg ggcggaagac gaaaatgccg tctgaaacca
    11641 gttcggcttg ataaaaccac gttttcagac agtcttttat agtagattaa aattgaaaac
    11701 ggtcatatcg ccattccctc gaaagcagga atccggaagt ttgaaatagc ggttaacctt
    11761 aaacatttcc aataaatcaa agtctggatt cccgcctgcg cgggaatgac ggcatagcgg
    11821 cttttccttt gcatttgcca tacacttgtt ttttgaaatc accgccgcca caccgcccga
    11881 caatattgaa acaccctgcc caccatttac cacgcacata acgccttatg atagacctgc
    11941 actgccattc caccgtttcc gacggtatgc tctcccccgc cgaaatcgtg cgcctcgcgc
    12001 atcaaaatgg ctgcacgctg ctggcgttga ccgaccacga ccacacgggc ggcatcgccg
    12061 aagcgcgtgc cgaagccgac aggctcggtt tgcgcctgat taacggcgtg gaaatctccg
    12121 taacgtggcg cgggcgtacc atacacgttg tcggtttgga tttcgacgag caggacgaga
    12181 acctgcaaaa cttgttggcg caagtgcggc aaggccgtct gaaacgtctt gaagccatcg
    12241 ccgacaagct cgaaaagaaa ggcatcggcg gcgcatacga cggcgcgctg gagttggcgg
    12301 caaacaaaga aatggtcagc cgcacccacg tcgccgagtt cctcatcaaa aacggatacg
    12361 tcaaaaacaa gcagcaggcg ttcaccaaat acttgggcga cggcaaatcc tgcgccgtcc
    12421 gacacgaatg ggcgacgctg gaagactgcg tctccgccgt caacggcgcg ggtgggttgg
    12481 cggtgattgc ccatccgatg cgctacgatt tgtccgccac cgccaagcgc aatctgtttg
    12541 aagaatttaa aaacctcggc ggcgcaggca tcgaagtcca cagcggaaac tgttgcaaaa
    12601 acgaccgcct caactacgcg cttttggcag aacgcttcgg tatgctcgcc agcgcgggca
    12661 gcgacttcca ccgtttaaac gacttcagcg gcggcatcct cggcgcgtgt cccgagctgc
    12721 cggaaaactg caggccggtg tgggcgcatt tttcccgaca ttgaatgcgg ataaaaatgc
    12781 cgtctgaaaa cctttcagac ggcatttttt tgcgttttaa acgttgtcgt acagttttcg
    12841 gacggtttta ccgatggcgg caatgccttt ttccagcgtt tgagcgtcct gcgcgatact
    12901 catgcggatg cactcgcccg cgtgcgggta atcctgcgtg tcgatgccga cgaagaaatg
    12961 ttcgcccgga ataatcagcg tgccttcggc tttgagcatt tcgtacaggg tttgcgaaga
    13021 aacgggcagg ttttcaaacc agagccacag gaaaatcgcg ccttcgggtt tgtggatttt
    13081 catcgggtac gcgcccagct cgcgcttgag cagcgagacg gcggtttgcg cctgattgcg
    13141 gtaaaacggc cggatgactt ggtcggcaag ccgtttcatc tcgccgcttt ccagtagcgg
    13201 ggcggcgatg gccgcgccga agcgcgtggg ggcaaggttc acaatcgcgt tcaggctgct
    13261 gacggctttg accacttccg gcgcggcgac gatgatgccg gtgcgcacgc ccggcaggcc
    13321 gactttggaa aggctgaagc agaggatgat gttttcgtgc caattcagcg tcacgtcgct
    13381 gtaaatgatg ttggggaacg gcattccgta ggcgttgtcg ataatcagcg gaatcccgtg
    13441 ttcacgcgcc aaagcgtcca aacgcgccat ttcgccgtcg gtcagcacgt tgccggtcgg
    13501 gttggtcggg cgcgaacagc aaatcgcgcc gattttgccc gctttgagtt cgggcaggtt
    13561 ttccagtgcg tcaaagtcca cgcggtattt gaagaagcct gcttcgcctt cgtgttcgac
    13621 gttttcgatt ttgggcttga cggaaacgaa gtgctgccct tcgacatgca cgtcggcata
    13681 gccgatgtat tcgggcgcga gcggcaacaa aatggctttt tctgcggatg tgccgtctga
    13741 aaggttgaat ttgccgccga agaggttgaa taaatagaaa aacgcgtttt gcgaaccgtt
    13801 ggtcagcgcg atattgtcgg cggtcaggtt ccagccgtat tcgcggttga ggaaggcggt
    13861 cagcgcgtca atcagcgcgg catctccttg aggattggag tagttgccga tgttttcgac
    13921 ggcatgttct gctgccagtt tggaaaatac gtcggcgaac acggcattga cttcggcgat
    13981 tcgcgccgga ttgccgccgc cgagcatatt gacgggcttg tcgcttttga gcgcgtcgcc
    14041 gaggtcgtcc atcagttgga ggatgccgct gtgttgcgtg aatttttcgc cgaatgccga
    14101 aaactgcata tcaaactccg tgtgtgtgtc aggcaggttg gtttgtacgt tatgccgtct
    14161 gaaggttcag acggcatttt ttctgtgtgt ttctcgtacc caaagcaggt cgcagatgcc
    14221 gtcgctcggg gtaaagccgg tcggggcgtt gatgagcagg gtgcggattg cctgctggtc
    14281 gtaacggtcg caggccgaac ggatgcggtc gagcaggaca gagagctcgt gccacggcag
    14341 catcgcctcg tcagccgtca taatgcgcgg atgggcggtt ttgcggacgt tgtcgccgat
    14401 gagcagctct tcgtagagtt tttcgccggg acgcagtccg gtaatgagga tttcgatgtc
    14461 gccgtcgggt tgttcgggtg tgttgggttt gaggccgctt agggtaatca tttggcgggc
    14521 aaggtcgatg attttgacgg attcacccat gtcgaggacg aatacgtcgc cgcccgtacc
    14581 catcgcgcct gcctgtatga cgagttgggc ggcttcgggt atggtcatga aataacgtgt
    14641 gatttcgggg tgggtcaggg taagcgggcc gccttctgca atctgttttt caaacagcgg
    14701 gacaacggag ccggacgaac ctaaaacatt gccgaaacgt accatgctga agcgggtttt
    14761 ttgtccgggt tcggcggcga gtgcctgaag gcagagttcc gccatgcgtt tgctggcacc
    14821 catggtgttg gtggggcgga cggctttgtc ggtggagatg aggacgaaag ttcttacgcc
    14881 cgatgtcgtg gcggcaagcg cgcactcgag tgtgccgaag atgttgttgc gtatgccttc
    14941 gacggtgttg aactcgacca tggggacgtg tttgtaggca gcggcgtgat agacggtcgc
    15001 aacggaaaag gcggtcatga cgtgttcgag cagcgtgcgg ttttgcaccg aaccgagaaa
    15061 gggcaggatt tcggtgtcga ggcgtttttg gatgcaggtt tcgcgcaatt ctttttcgat
    15121 ggcgtacagg gcgaattcgg ataactcgaa cagcagcagc ttttcggggc ggcggcggat
    15181 aatctggcgg cagagttccg aaccgatgga gccgcccgcg ccggttacca tgacggtttt
    15241 gccttcgatg tcggcactca tcaggcggtc gtcgggcgcg acggaatcac gcccgagcag
    15301 gtcggacaca gagatttttt tgagcgtgcc gatgctgatt tttccgtcca tcaggtcttt
    15361 cattccggga atggtcaaca cttcgcacgg ataggcttcc agtttgttga tgattcggcg
    15421 gcgttgttcc tgggtcgcgc cggggatggc gagcaggatt ttttccacgc cgtagcgttc
    15481 gatgaggaag gcgatggcat cgggctggta aacggcaagg tcgtagatga cggtgtgcca
    15541 cagtttgggg tcgtcgtcta caaaggcggc ggcggaatat tcgcgcattt gtttgacggc
    15601 ctcaagcagt tgtctgcccg accgtcccgc gccgtaaatg atgacaggga tcatctgttt
    15661 tttggggtgt tcggacaaca gtccgcgcaa aaccatacgc gagccggtca cggaaacaaa
    15721 cagcagtaag aaatagacaa tcggcagggc gaggcgcagc ctttcttcaa aaatcaatgt
    15781 gttgaggaag aacaggacgg cggaaacaat gctgccggca aaagcggtgg tcaggacgtg
    15841 gaaactgaca aagcgtgtaa cggcacggta gagccccatt ctgacgaaca ggccgatggt
    15901 caacagcgcg gtcaatagga aagactgcca gttggcaggg tcgaaccatt cgttcgagta
    15961 gtcggccttc aggctttggg taaaccaaaa ggcaatgaaa atcatcagaa aatcgtgtat
    16021 gaggaaacag attttcttga tgttgcgcgg tagggcaagc agggtttcca gagtcatatc
    16081 gtggggcggt atgtgctttc aggcggcata tgccgtctga agggttatcg tgtggcttcg
    16141 gtcaatacgg cttcgatgtg ttctttgcag aacgcgattt cgccgtcggt cagcgtcgga
    16201 tgtactaaga acatcaggct ggtgtcgccc aactcgacag catttttcag ccgctctttc
    16261 ggacgccaag gcgtgttgtc gaaggatttc tccaaataca cttcggagca gctaccttga
    16321 tagcagggga ctttgcgcgc gttcagttcg ttgacgatgc ggtcgcgcgt ccagccgtct
    16381 ttgaggtgtt cgggtttgac gaaggcgtag aacttatatt gcgcgtgtcc gatgtagtcg
    16441 gcgacttcaa ccaagcggat acttttgaat ttgcctaggc tttccgccag cttggcggcg
    16501 tgcgcttgac ggcaggcagt ccattcgggc aggcgtttga gctgaatgcg cccgattgcc
    16561 gcctgcattt ccatcatacg ccagtttgtg ccgaagcttt cgtgcagcca gcggaagcca
    16621 ggggcgtgtt cgcggtggta cacggcgtca tagcttttgc cgtggtcttt gtacgaccac
    16681 attttgctcc acagtgcttt gtcgttggtg gtaaccatgc cgccttcgcc gcccgtggtc
    16741 atgattttgt cttggcagaa cgaccatgcg ccgacgtgtc cgatgctgcc gacggatttg
    16801 cctttgtatt tcgcgccgtg cgcttgggcg cagtcttcaa tcacccacaa atcatgttct
    16861 tttgccaaag ccataatgcc gtccatttcg gcaggcatac cggcaaggtg aaccacgata
    16921 atcgctttgg tattcggggt cagcgcggct ttgacggttt ccgcgctgat gttttggctg
    16981 ttcaaatcca catcagcaaa cacgggattt gcgcccgcgt tcacaatgca ggaagcggaa
    17041 gccaagaaag tgcgcgaggt aacaatcaca tcgttgcccg cgcctatgcc gattgctttg
    17101 agcgcgacat cgagtgccag cgtgccgttg gaaagggcga cggcgtaccg cgtgccggca
    17161 aaggcggcaa attctttttc aaattcgcgg cattcgttgc ccgtccagta gttgactttg
    17221 ttggacagca ggactttgga aacggcatcg gcttcttctt gggtgaagca gggccacggg
    17281 gaaagggcgg tgttcagcat gatggtttgt ccgtcggttt tcagacggca tttcctcatt
    17341 tctgccgtct gaagggggtg tgttccgaag aatcgggcgc acgccgcagg tgttgtcaaa
    17401 atcggtctgt acgggggtgt atagtggatt aaatttaaac cagtacggcg ttgcctcgcc
    17461 ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgattta aatttaatcc
    17521 actatatttt aatcgcttat gccgtcccgg tcttggggtt tttgcgcggt agcggctttg
    17581 ccggattgcc cgcgacggtc atgccgtccg ggatgtcgca cacgataacc gcccccgcgc
    17641 ccattcaact tccatcgttt ccaaatggtg tcgaaaacgt agggcggaaa gccgtttacg
    17701 ccccatgaat aatattttgt catgaaatag tccttttaat attgtgagtg catgtttgat
    17761 tatgtaatgg ttaagtattt gaatcaatta taggaactaa atcttgaagg gagttaaccc
    17821 aaaagtcggg atgatattct gtgttaaaac tttcaggctg gtggcggtgg atgttctgtc
    17881 cggagggaag caatcctatg gttatccagc ccaaagtgtt cggcgcgata aagtctttgg
    17941 aaatgttgtc gccgatatag atgaagcagc ctgccttgtc ggcatatttg tcttgaagat
    18001 ggcggaagcg tttgctgtcg ggcttttccg aagagcaggc ttcagagatg aggatgtcgt
    18061 caaacaggga agacagaccc aaggcttcca gcttcaggcg ttgggtcagg cttcttccgt
    18121 cggtaatcag cgcgcgtgcg gcgaagggcg cggtcagttc ggacaggaaa tccggcggag
    18181 catacggagt cagcgtcggg cggtgcagcc ggtattgcca caaaagaacc tgtttttccg
    18241 attcattaaa accacagtgg cggcacaatt tgtccagcca atctttgccg tcggggtcga
    18301 tgttgcgcca taggttgtct gaattccaat cgggatacag tgcggcaacg gtatcgatga
    18361 ccgaacggat accggagagc ttgtattcgt attccgaata cagggtatcg tccaagtcga
    18421 agacgatgac ggtggtttca ggattaatcg ttttcatgaa ccaagacctt ggcatcgtag
    18481 cgcaacataa tgaggttgtt ttcccagtcg tctgaaaacg ggatttcttc gtcgccgagg
    18541 tattcgcgca tcaaccggct cggataattg ccgcctgcgg catacgtcag cgggaagcca
    18601 ccgccgaagc gggggttgat ttccacgccg tagaattcgc cggtttcttc gttgtagaag
    18661 aactgcaccg tgatacagcc gcgcgcgccc tgcaataccg acattttttc cagcagcgtt
    18721 tggtagaggc tgtttttgca ggtaacgcct ttgctgattt cgccggagcg cacttccaag
    18781 cgttttcgcg ggatggcgca tttcagacgg cattgacggt cgtaatacat atcgacggta
    18841 aactcggaaa atttattttc gatgtcgata aactgacaga acattaattt ggtgtctgcc
    18901 aagacttccg gtgtcaggtc ggcaggtgtg tcaatccgtt ttgcaccgat ggcgcggctg
    18961 ccgtcataag gcttggcgaa gcaagggaaa accaacttct tgcggtcgta aatttcgggt
    19021 gagcgtatgc cgtagcgggc aaacaaggtg gcggttttcc gtttgtcgcg gcattgtgcg
    19081 atgaagtccg aatcggaaac gacgatgtgg acacttgccg cttcaaagcg ttcgcgctcg
    19141 tctgccagct tttgcagttc ggtgtctatg gttgggatga ttaaaccgat attttcgcgt
    19201 accgccagat tgaaaatgct gtcgatatat gaatcggtac taatcggcgg cacttcaaat
    19261 gaaccgtccg aaacatggca ggcagaagac atgcgggggt tgaggtcagt ggcaaaaacg
    19321 ctgatgccgt ctgaaaaacg tgcggcttcg gtttggaagt cctgcaccaa ttcgacacgg
    19381 cgacctgccg acagaatcag tatattattt ttttcattag tttgatttcc agtgaaataa
    19441 ggcatagtgg cttccccttc cgccgaaatc ccttctttga taaagacttt tctgactgtc
    19501 aaaaacagga ttttcatatc cagccaaaaa ctgtaatggt cgatatacca aacatcgcag
    19561 gcgaattttt cgtcccacga aagcgcgttg cgcccgttga cctgcgccca gccggtaatg
    19621 ccgggtttca tttcgtggcg gcggttttgg aagttgtcgt acagcggcag atattgcatc
    19681 agcagcgggc gcgggccgac caggctcatg tcgcctttta agacattcca taattcaggc
    19741 agttcgtcca aactagcggc acgcagtttt ttgccgaagg gtgtgagccg ctcgctgtcg
    19801 ggcagcggaa tgccgtctga atcaagcgcg tcgcgcatgg aacggaattt gaccatttta
    19861 aaaggttttc cgtcctttcc ggggcgttct tgaatgaaga agacgggcga acccagattc
    19921 ttgcggatga ggtacgccaa aatcaggaaa acgggtgaga ggacaatcag ccccgatgcg
    19981 gatgcgatga tgtcgaacag gcgtttgacg gctttactca tttgccaatc tttcaatcag
    20041 gttgacgatt ttttgatagg caacgtcgcg tttgaagcgg cggacgattt cgtcggactg
    20101 aacggggtcg tttttgcgtt tcaaaatatc tttggcggct tgaacgaagc tgtccacgtc
    20161 gccggaacgg tagttttcgt gcggtagtag gttcaagagg ccgaggactt cggcattggt
    20221 ctggctgttt aaaatcggtt tttgcaaagc catatagtcg gagagtttgt tggtaaccga
    20281 ctgcatggcg tggctatgga tggcgttgac ggcaatgtcg cacgctttgg caatcgacat
    20341 catttcagaa tagggtaaat aaccgtaaaa cttgatggcg cgattttcat attgtttgag
    20401 tttctccaaa tcgggtcctc cgcccataat gtgcaattcg acgttttcgc cgtcgtctaa
    20461 aagtttccga acgcctttgc acacggtttc cacgtcgtag ctgtaactga gcgtgcccaa
    20521 gtagaaaagg cggacggttt tggagcgg
//
LOCUS       DAWFJW010000025        20221 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFJW010000025 DAWFJW010000000
VERSION     DAWFJW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.025"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(209..284)
                     /locus_tag="WGR19_000752"
     tRNA            complement(209..284)
                     /locus_tag="WGR19_000752"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Asn,seq:gtt)
     gene            complement(377..736)
                     /locus_tag="WGR19_000753"
     CDS             complement(377..736)
                     /locus_tag="WGR19_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0798313.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(759..1403)
                     /locus_tag="WGR19_000754"
     CDS             complement(759..1403)
                     /locus_tag="WGR19_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0798314.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1405..1941)
                     /locus_tag="WGR19_000755"
     CDS             complement(1405..1941)
                     /locus_tag="WGR19_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0798315.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1953..3050)
                     /locus_tag="WGR19_000756"
     CDS             complement(1953..3050)
                     /locus_tag="WGR19_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0798316.1"
                     /translation="MEVQLPKIKTVRVMLTGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3053..4489)
                     /locus_tag="WGR19_000757"
     CDS             complement(3053..4489)
                     /locus_tag="WGR19_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0798317.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLILSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WGR19_000758"
     CDS             complement(4709..5491)
                     /gene="bioC"
                     /locus_tag="WGR19_000758"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0798318.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5479..6126)
                     /locus_tag="WGR19_000759"
     CDS             complement(5479..6126)
                     /locus_tag="WGR19_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0798319.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGISLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFSARPFDEIHQELIALFAMIGQDRR
                     TDLIRWTNALVGSGDKIFMSANQHRYWTARCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WGR19_000760"
     CDS             complement(6136..7278)
                     /gene="bioF"
                     /locus_tag="WGR19_000760"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0798320.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            complement(7300..7437)
                     /locus_tag="WGR19_000761"
     CDS             complement(7300..7437)
                     /locus_tag="WGR19_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ0798321.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7501..8575
                     /locus_tag="WGR19_000762"
                     /pseudo
     CDS             7501..8575
                     /locus_tag="WGR19_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
     gene            complement(8782..10248)
                     /locus_tag="WGR19_000763"
     CDS             complement(8782..10248)
                     /locus_tag="WGR19_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0798322.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10446..11369)
                     /gene="speB"
                     /locus_tag="WGR19_000764"
     CDS             complement(10446..11369)
                     /gene="speB"
                     /locus_tag="WGR19_000764"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0798323.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11468..13360)
                     /gene="speA"
                     /locus_tag="WGR19_000765"
     CDS             complement(11468..13360)
                     /gene="speA"
                     /locus_tag="WGR19_000765"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0798324.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYASTVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13527..13811)
                     /locus_tag="WGR19_000766"
     CDS             complement(13527..13811)
                     /locus_tag="WGR19_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0798325.1"
                     /translation="MKKLLLAAVVSLSAATAFASDSAERQIYSDPNFEQNRVKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13962..15770)
                     /gene="aspS"
                     /locus_tag="WGR19_000767"
     CDS             complement(13962..15770)
                     /gene="aspS"
                     /locus_tag="WGR19_000767"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0798326.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15828..16529)
                     /locus_tag="WGR19_000768"
     CDS             complement(15828..16529)
                     /locus_tag="WGR19_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0798327.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16672..17820)
                     /locus_tag="WGR19_000769"
     CDS             complement(16672..17820)
                     /locus_tag="WGR19_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0798328.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGRLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17980..18243)
                     /gene="rpsT"
                     /locus_tag="WGR19_000770"
     CDS             complement(17980..18243)
                     /gene="rpsT"
                     /locus_tag="WGR19_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0798329.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18546..19688
                     /locus_tag="WGR19_000771"
     CDS             18546..19688
                     /locus_tag="WGR19_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0798330.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5215 a         5219 c         4677 g         5110 t
ORIGIN      
        1 gcgagccaat gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagctg
       61 tccaggacag cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt
      121 ccatctcaaa acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct
      181 aagaaaatac ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc
      241 tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata
      301 aaacagaaat accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc
      361 ccgcaatctt tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca
      421 ataggattaa accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac
      481 ccgcctgact taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc
      541 cgcaggcgag ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc
      601 ctaccctgtc cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg
      661 agataacggc agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa
      721 tcaataattg ctgcataacc ccttctctct cccaagtttt acacgcggtt gctgtaacgg
      781 cggtataaaa tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg
      841 ataaccaaaa taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga
      901 ccggaaggat cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc
      961 agccggtatt tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc
     1021 gcctcatgat ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc
     1081 ttaccgctgc cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg
     1141 ccgtctgaat gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac
     1201 aggttcaaag cggtatcgat gtcggcggtg tccgcccagg taagcagctt ctcactgctg
     1261 accatttcgt ccgcatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg
     1321 gcatcgctgg atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct
     1381 tgtagtgaaa gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat
     1441 tgcgctaacc aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac
     1501 attaagatta tgttttgcaa gatatttgtg atacacgtcg ataccgggct gagagcgtat
     1561 atccatcttg gtaataccga caacgacggg cgccttctcc agcagccctc gaaacgaatg
     1621 taaaaagaat tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa
     1681 acccatactg ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt
     1741 accatataaa tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac
     1801 tgtcgtattc cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag
     1861 tgcttcgtcc gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa
     1921 aataatttta ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc
     1981 ctttgcagaa gaccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc
     2041 atcatttcac tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag
     2101 tatgcctgtt gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc
     2161 gcaagataat tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg
     2221 ttgacggatg ccttggtcag aaatgccgac aagcgtatcg accccggcac atttgccaac
     2281 cgggtcaggt ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc
     2341 aacctgccct gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta
     2401 gccttttcgc gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg
     2461 tctttgcaca attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag
     2521 gggaaaacaa taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt
     2581 tccgcaaacc gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc
     2641 gtacgctgca cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc
     2701 ccataaacat tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga
     2761 atcggtttgg gcaagtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg
     2821 ggtatatcct tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg
     2881 gtatccgcca aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa
     2941 cgggtggtat tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc
     3001 cccgtcaaca ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc
     3061 cgtaccgttt tattttttta atattttgat tcatacgctg cagcagtcgg ctcatcagca
     3121 taacgacctc ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca
     3181 acctatccca atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact
     3241 cgtcgggata tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat
     3301 acatcagcaa cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttga
     3361 tcaacgaaga atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt
     3421 tcggcagata cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt
     3481 ccaaatcatc gaaaacttca tggtaaccca ggctgtaccc ccaccccagc atccgctctt
     3541 taacctgcct gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac
     3601 ggtctatgtc cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt
     3661 tttcggaagt ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt
     3721 ctttaaggat tttcgcacct tgatcggcac ggaccaaacc ggcaatcgca ccgatttcct
     3781 cttgactgat tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt
     3841 cggcttcata agccttaccg ccggcatccg gcgggggcga tgcggaagct gccgtcgcag
     3901 aaccgccttc cgcacgtttt tcaatctcct gagtacccca tcccaaacct tcttccgcca
     3961 agacgcggat acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca
     4021 aaagttcgga aagaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat
     4081 cccccacttc gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac
     4141 catccggaat gccgtccaga taggcagcca aagattcggc agccttgccc tgataaccga
     4201 attgcttata aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg
     4261 atgcggtctc ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt
     4321 gtccaatctg atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc
     4381 cgacaccttg ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa
     4441 gcatcagcaa taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc
     4501 gataccgtaa cgagcggata gctgaccgcc tgcaacggcc attgtccgct tttattatta
     4561 acgatttact gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat
     4621 atgccttctg cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat
     4681 ataaaaataa ctttgcccat aagctgcctt attgcctgcc cgccgcagag tagcgcgcga
     4741 taaaaaataa cggatgataa gtcaggtgca ctttgcccga cggcatactg aacgccgaca
     4801 agtaatcgcc aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggctg
     4861 ttacgcctgt atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta
     4921 ccttaaaatc ctcacaccat aaaagctcaa aatctttggc taaccaagtc tgccattggg
     4981 ataaagtcgg gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt
     5041 tgccaaaggt tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg caatgagcga
     5101 taaaagcgtc gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt
     5161 gtcgttgaaa aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt
     5221 tttcagccag ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa
     5281 tctgtttttg cagcaaggca ctcaacattc ctgagccaca gcccaattcc aacacgtttt
     5341 ccaatggcat atccggcaaa taatcttgca aatgcgccat taaattaatc gtcatttttt
     5401 gttggattaa ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc
     5461 gcgatttatt tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa
     5521 caggtaatgt ccgccgtcaa tttcccgaac ggtgcaacgc gctgtccaat atcggtgctg
     5581 attggcagac ataaaaattt tatcgcccga tccgaccaag gcatttgtcc agcgaatcaa
     5641 atctgtgcgc ctatcttgcc cgatcatcgc aaaaagtgcg ataagttctt gatgaatttc
     5701 gtcaaacgga cgggcggaaa attgttggta atcttcaaaa gatgctttat cgccacacat
     5761 tctgcgttca aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac
     5821 ggcgcaaggg ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc
     5881 ggattttaaa cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc
     5941 caccaaacgg atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc
     6001 atagcaaatc aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg
     6061 cgttccccaa cctgcaaaat acaggattaa atgtccgcct tgatgattgt aaaattttgt
     6121 tttcatatca tatccttaca ggcacgccgc aaactgccgc acttcatcca ttgtcatatc
     6181 tgccgtcaaa gacaatcgta ttctggatgt accttttggt actgtcggtg gtctgatggg
     6241 caggcaataa taaccctgcc tttgcaggta ttccgctttg gcaagggtgg cttcattccc
     6301 gcctaaaata taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac
     6361 ttcccgccgt aaaaatgcgc ttaactgctc aagatggctt ctttcttttg agaattgcgg
     6421 caatcgttca aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa
     6481 aatcaatggg cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata
     6541 cgcccccacc gaggctaagg ctttaccgaa agtgccgacc aataaatcaa tctcggcaat
     6601 caaattatcc cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgagc
     6661 ttcatccaca taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa
     6721 atccgccaca tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa
     6781 ttttccgacg tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg
     6841 gaaaaacgca caccggctca aacggatgcc gtcaatcata ctggcgtgaa caaatttatc
     6901 tgccaaaatc aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata
     6961 gccgctgttg aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa
     7021 atcggtataa ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg
     7081 aaaattaccg ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc
     7141 caaataatca ttagacgaca tattcagcat tttgcggttt tcccgcgtaa tataccgccc
     7201 ttgatgaatc aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg
     7261 ttgtttaaaa accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag
     7321 catcaaacaa tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga cctttgccac
     7381 acccctgcct tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac
     7441 ccaatccgtc cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt
     7501 atgatttgcc gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc
     7561 cgcactgctg cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc
     7621 tcccccgtcg cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc
     7681 tacaccgacg gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa
     7741 accaatctag gcgaacataa aatcggtgcg aaagcctatc cctgtttggt acatccccaa
     7801 atccatacag aaagaacgcg gcgacggcgt aaaaaccata gccgccggcc cggacaaccc
     7861 tttggggccg gtttttgtcc gcttaggcga ccctaaactc ggtttgggca tacacgggac
     7921 caatgcgccg gccagcgtcc ccggggttcg gagtcacggc tgcgtccgca tgaaatcgcc
     7981 cgacgcgctc gagtttgcca aaaccatcgc cagcggctcg cccgcctccg tcatctatca
     8041 aatggcgggt ctcaatgaag atgcggatcg caacctgtgg cttgccgcct tccgcgaccc
     8101 ttacggtaag aacaaccttg acatcgcctc tctgaaaaaa agtattgcgc aatgggcaaa
     8161 aacacagggt aaaaccatcg cgcccgagaa agtcgatgct gtactcaaag accgcaccgg
     8221 atcggccgtc tgcctgacct gcggcaaaaa cggcaagatg aagatgccgc tcaaatcgct
     8281 ggcgtggata cagggttctt cctcatacag ccaagctgaa gtcattgaac aaactgagga
     8341 aaccaactct gccgaaatat ccgagacacg cacgcccgaa gtgcctgatg tacacacgcc
     8401 cgaggcacaa ccgcatttaa atacccaatc cgacggcacg ccgactgcct ataccgaacc
     8461 ggctgccgat tcatcgccgc aagtagaaac acctgatcag gctgcttccg agccggttga
     8521 tgtattattt tcaatagatg tgatacggca gggaaatttg cgtttaggta attgaataac
     8581 cttctgatta tttaatgctt atgtttatca gaagttgata ggcggtttgg tttttgtttg
     8641 gcttttccta caccgccgcc tgccgctttc tgcaacattc aagcgcacaa atatgccatc
     8701 tgaaggcttt agacggcata tttcacgata atagtcgccc aatcaaataa acacagattg
     8761 gaaatttatt ttatttttcc cttaccaata ccccagaact ttccaccaaa tgctgccgat
     8821 aacgaaaaaa atcagaaaat tgactacgct catgataaaa cccgccttcc accattctcc
     8881 cattgtggtg tagcccgaac cgaaaatcac aggcgaagta ccggtcgcat aatgagtgag
     8941 ggtcatcata atgttagatg cggccgccat catcagcgcg gtcggcatcg ccggggcatt
     9001 cagtgaaacg gcagcagcga aaaatgcgcc gaacatagcg gtaatatgtg cagtagtact
     9061 ggcaaacata taatgcgcat acatataagc aagcacgagg attacgcccg cagccgtgcc
     9121 gctaacgccc aaaccgccga cactttccgc caacactccg gagaaccatt taatcagtcc
     9181 gagtttattt aaaaatgcgg ccatcataat caatgcgcca aaccaaataa tcgtatccca
     9241 cgcacttttt tctttcaaaa catcgtccca agtcaataca ccggaaagca aaagcaggct
     9301 taatccgata aatgcggtgg cggtggcgtt gatactaaaa gcgtgattgc cggtaataag
     9361 ggcgggaaca tctgcccaca acagcagcaa gataccgaaa atgaccgcca taatgatttc
     9421 gtctgccgac attttaccca tctccctcag acggtctttg gcaaattgaa cggcattggg
     9481 cgtttcttta atttcaggcg gatacaaaaa atataaaatc aaaggcataa cgaaaaaggc
     9541 gataacgccg ggaacagcca ttgcccacgc ccacgccccc caagaaagac ggaaactact
     9601 gcctaaattt tcggcaatca agttgacgat taaagggttg ggggcagttg cagtaataaa
     9661 catagccgac gaaatgggat tggaatgata gttgaccaaa gccaaatatt tacccatctt
     9721 gccttctgtg ccttttgcgg gattggagcc gtaactgccg gcaatcgact gcataatcgg
     9781 atgtataatg ccgccgccgc gcgcggtatt ggaaggggta acgggagcca gcagcagttc
     9841 ggaaagagcg agactgtaac cgatgcccag cgtttttctt ccaaaaacgg cgataaacaa
     9901 atatccgata cgcatcccca gccctgtttt gagcaaaccg cgcgaaatca taactgcgat
     9961 ggcaatcagc caaatcaacg gattggcgaa cgcactcaac gcatcgctca tcgccgcgcc
    10021 cggtttgtcg gcggttacgc cggttactgc gaccaacccg acggcaataa tcgacagcgc
    10081 gcccaacggc atggccttgc cgataatggc ggcaatcaca ccgacaaaca tggccagcag
    10141 cgtccaagcc tgaggcttga ccccgtcggg tacgggcagt gccaaaacca gggcgcacaa
    10201 tactgcggca atggcgaggg gtatcggttt gaaacccaat ttcatcatat tgacctccgt
    10261 aaaaaagacc gtcccgaaaa atcggaaaaa taatatttaa ctaattgttt tataagatat
    10321 attcagatat ttcaccgtct ttccgatatg cggctccggg caacttttgt ttcagtattt
    10381 gaattttcat tagactgaat acgccgtttg aacggcacgg cgaaaacccg ggggatgccg
    10441 gacgttcagt cctttttcgc accttgaagg taaagcattt ccaaggcaat tgtggcgccg
    10501 gccaaagcgg taatgtcgga ttggtcgtaa gagggggcaa cttctacaac atccataccg
    10561 acgatgtcga gatccgtcag cccacgtagg atttttaatg ccctgtcgct gctcaagccg
    10621 ccgcatacgg gcgtaccggt cccgggggcg aacgacgggt cgaggcagtc tatgtcgaaa
    10681 gtcaggtaaa cgggcatatt gccgacggtt tctttgattt tacggacggt ctcttcaaca
    10741 ctgtcttcat taacttgggg ggcggtcaac acagtaaaag gcaatttttt actgtgttcg
    10801 gtacgtatgc cgatttgtac ggaacgggac gggtcgatga ggccttcctt gggggcggta
    10861 tagaacatcg taccgtggtc gtattcgctg ccgttgtcgt aggtgtcggt gtgcgcgtca
    10921 aaatgaatca gtgcgagttt gccgaaatag cgggcgtggg cgcgcaacaa cgggagggta
    10981 atgaaatggt cgccgcccaa actcaaacag cgtttgccgg aagaaagtaa tttgccggcg
    11041 tgcgcttcca ttttttcgac aaaatccctg ctgtcgccaa aagaaaaaac caagtcgccg
    11101 caatcaataa tgttcaggcg ttcgcgcaca tcaaatgtcc atggaaacct gcggtgctcc
    11161 caagcgaggt tgacggaggc gcgccggatg gcttcaggac cgaaacgcgc gccggaacgc
    11221 cctgaaaccg ccatatcata aggcacgccg gtaataaccc aatcggcatg actttcatac
    11281 ggcataaaat taagcggcag gcgcaaaaac ccgaaattat tggaaacgag ggagttgtcg
    11341 gtttgtcctg ccagtgtgct gtattgcatc gtaatgattc cttgtaattg gtttcaatcg
    11401 gtcgtgatga ttggtgtttg agtaagaaaa tcgggcttca gacgacatat ccgatgcctt
    11461 gatgcgtcta ttcgtcttcc aaataggtat aaccattaag ccccgcttcg agttctttta
    11521 agaaagacat agcctgcgag gcaggaaggt ctgaatgttc gatttgttcg cgatagcgtt
    11581 tcatcagctc tttcggatct tgataaacgt attcgagcat atcggcaacg gtgtttcctt
    11641 catcgtaatc gatgacggta aattgtccgt cttcccctac aacaacatcg gcagtggcag
    11701 tgtcgccgaa aagattgtgc atattgccga gtatttcctg atatgctccc accataaaaa
    11761 agcctaaaag cggcggctct tcttcgggat aatcaggcat aggcatcgta ccggcgatgc
    11821 cgtcgccgtc gatgtagtgg tcaatcgtac cgtctgaatc gcaggtaatg tccaacaaca
    11881 cggcgcggcg cgcaatcggt tcattcaaac cggtaatggg acaaacaggg aaaagttgat
    11941 ctatgcccca agcatcgggc aaagattgga agagtgagaa attgacatac agcttatcgg
    12001 caaaacgttc ttgcaattcg tcaataatgg ttcggtgaga ccggtgtttt tcattaaaca
    12061 attcgccgac ttcatgacag atatttaaat acagttgctc cgcccacgca cgttgcgcca
    12121 aactcaacag cccgacatta tactgattat gcacatcggc aagatcaaac tgcccttcgt
    12181 gtatccagct gcgtaaggaa cgtttttccc gcgaggcgga aatatccgtc caagtttccc
    12241 acatactgtg caacacacgc ggtgcttcgg gcgatggcgc atccagccgg cgcggtttgt
    12301 aacgttcaac gcctataaca ttagcaacca aaacggcgtg atgtgcggta atgccgcgcc
    12361 cgctctcggt aatgattgtc ggatgcggca gcccgtgttc gagacaagcc tggctgatgc
    12421 cccatacgac tgtggaggca tattcgttga ggctgtaatt aacggaacaa tccgattgtg
    12481 tgcggtttcc ttcgtaatcc acgccaagcc cgccgcctac atcaaaacag cggatattta
    12541 cccccagttt gtgcaactca acataaaacc gagccgattc gtgtacacct gtggcaacat
    12601 cacggatgtt cccaagctgc gagcctaaat ggaaatgcaa aagctgcagg cagtccagcc
    12661 tgtttttttg tttcaaaata tcgaccagtt gcaaaacttg ggaagccgac aagccgaatt
    12721 ttgatttttc cccacccgaa gactgccatt ttcccgaacc ttgggaagcc agtctggcgc
    12781 gcacacccaa acggggcttg atgccgagtt tttccgcctc ttccaatacc atttgtattt
    12841 cggacagctt ctcaatcacc aaataaacct gatgccccag tttttcgccc atcaaggcga
    12901 aacggatata ttcacggtct ttatagccgt tgcagacgat taatgtttgc cgggtgccgg
    12961 cgtgtgccaa aaccgccatc agttcggctt tagaaccagc ttccaaacca tgcggttgtc
    13021 cgcttgacat aagcgattcg atgacgcggc ggtgttggtt gaccttgata gggtaaacca
    13081 aacaataacc gcccttatag ccgcactctt cccgtgccgt ctgaaaggcg cggttaatgt
    13141 cgcggaggcg gtgttcgagg atttgcggaa aacaaaacaa aacaggcagg cgcgcctgat
    13201 gtttttgttg cacggcttca gtcagttttt gcagtgaaac agtttgattg tgttgcgagg
    13261 gattggggcg gacgatgatt tcgccggaat cgtcaacatc ataataacct atgccccaat
    13321 gattaatgtt gcacacttca cggatggtaa ggataggcat aataaacctg ctccgtctgt
    13381 cgtgttgaaa aggaatgatt ataacaaatc agcgtgaaat gtcatttttt taataagaaa
    13441 agcctgcctc atacctgatg aggaacaggc aaaatgccgt ctgaacgctt cagacggcat
    13501 tttggttcat ctttccatca aaggagtcag cgatcgagct gctctttgat gattttcagg
    13561 tcggggtaag acaacacgat gtcgtattcg cggccgtctt tataggcttc cacttccagc
    13621 acaggcttac cccaatggtc gtcggcatcg acatcgtgaa cctgataacc gcgctgttcc
    13681 aacattttca cagcttttac gcggttctgt tcaaaattgg gatcactgta aatctgacgc
    13741 tcggcagagt cgctggcaaa tgcggttgcg gcactcaggg aaacaacggc agccaataac
    13801 agttttttca ttttcagtcc tttctttatc ggttgattga ataagatgtg tttttcaatg
    13861 ccgccattaa aacacagcaa aattaggttt gaattagagg gaagtcaaga agcgtaaatg
    13921 ccgtctgaaa aaaacagagc cgtcaaacgg ctccgttttc cttatgcttc tttggtttca
    13981 gccgcctttt ggcgcaggcg caggctcaat tcacgcaact gcttgtcgtc cacgctgttg
    14041 ggcgcgttgg tcagcaggca ttgggcacgt tgtgttttcg ggaaggcaat cacgtcgcgg
    14101 atagattcgg cgccggtcat cagggtaacc aaacggtcga ggccgaatgc aaggccgccg
    14161 tgaggaggcg caccgaattt caggttgtcc aagaggaagc cgaatttctc ttgttgctct
    14221 tcagggctga ttttcagcgc ggcaaacact ttctcttgta cgtctgcacg gtggatacgg
    14281 atagagccgc cgccgatttc ccagccgttc aataccatgt cgtaggcacg ggccaggcag
    14341 tttgcagggt cggaaaccat caggtcttca tgaccttctt taggcgcggt aaacggatgg
    14401 tgtacggcaa cgtagcggtc ggcttcttcg tcgtattcga acattgggaa atcgacaacc
    14461 cacaaaggtt tccattcgtc tgtgaagtag ccgttgtctt tgccgtgttc caagccgact
    14521 ttgatacgca atgcgccgat ggcttcgttc acaactttgg ttttgtccgc accgaagaaa
    14581 ataatatcgc cgttttgtgc gccggtacgc tcgatgattt ctttcagggc attctcagac
    14641 aggaatttta cgattggaga ttgcaggccg ctgtcttcgc cgtttgagag gttgcccaca
    14701 tcgtttactt tgatgtatgc cagacctttc gcgccgtaga tgccgacaaa tttagtgtat
    14761 tcgtcgattt ctttgcggct gaattttgcg ccgttcggta cgcgcagggc gactacacgg
    14821 ccgcctttca tatctgccgc gccacggaag actttgaatt cttccgtttt catcaggtcg
    14881 gtcaactcgg taaatttcaa gttgatacgc atatccggtt tgtcagagcc gtagtagaac
    14941 atggcttcgg cgtaaggcat acgcgggaag tcgcctaaat ctacgtttaa agcatctttg
    15001 aagacttgtt tcgccatacc ttcggtgatg tccatgattt catcctcgtt caagaacgag
    15061 gtttccaagt caatttgcgt gaattcaggc tgacggtcgg cacgcaggtc ttcgtcgcgg
    15121 aagcacttgg tgatttggta gtaacggtcg aaacccgcca ccatcaacag ttgtttgaat
    15181 aattgcggcg attgcggtag cgcgaaaaac tcgcccggat gaacgcggct cggcacgagg
    15241 tagtcgcgcg cgccttcagg cgtggagcgg gtcagcatcg gggtttcaat gtcgatgaaa
    15301 ccttgcgcgt ccaagtagcg gcgaacgccc atagccactt ggtaacgcag gcgcaggttg
    15361 cgctgcatca ccggacggcg caagtcgata acgcggttgg tcaggcgcac gttttcgctg
    15421 atgttttcat cgtcgatttg gaacggcggc gtggcggcag cgttcaagac ttcgatttct
    15481 ttggcaagaa tctcgatttt gccggaaatc attttgtcgt tggtcgtacc ttcgggacgg
    15541 ttgcgtacgc ggccggtaat gctcaaaacg tattcgttgc gagcggaatc ggcagcggca
    15601 aacgcttcgg gcgtgtcggg gtcaatcacg acttggacga tgccttcgcg gtcgcgcagg
    15661 tcgataaaaa tcacaccgcc gtggtcgcgc cgacggtgta cccagccttt gacggtaacg
    15721 gtttggtcta agtattgctc actgatcaga ccgcaatagt tggtacgcat aaaatcacct
    15781 tttattgatt taaactgaaa acagaaaatg ccgtctgaac ggcggcttta ttgttgttcg
    15841 ggcaaatccg ccttttcaga cggcataggt cctgccaatg ttttgacggg caggtcgtca
    15901 gggatgacca tacccagcga aatcacatat ttcaacgctt cgtccacgct catatcgagt
    15961 tcgcgcacat cgcttttctt taccataata tagtaaccgc cggtcgggtt gggcgtggtc
    16021 gggacataca cggaaagata atcgccatcc tgcggcaatg cggccttaac cgcattcgac
    16081 acctgaccgg acacgaatgc gattgtccaa atacccgatt ggggaaacgg cacgagtact
    16141 ggtgttttaa acgaacggct gctgtcggac agcagcgatt cggatacttt tttcacactc
    16201 gaatagatgg acttcacaac cggaatccgc cccaacaggc tgtcccacgc ggcaagaatc
    16261 tgccggccca atacgtttgc ggcaaacaat ccggttacaa acaatacggc aatggcaacg
    16321 ataacgccca gccccgggat attaaacccc aaaacatatt gcggccgcca ttgcttcggc
    16381 agcaggttga cgagctgatc ggacgcggaa acgatatagg aaaccaccca aaccgttacc
    16441 gcaatcggca gccagaccaa aatgcccgta atcagatatt tttttaacgc cttggcagct
    16501 ttgccgcctt cggccgcagg ttccgtcatc ttgcttgatt ccgacaaagt ccgtacaaac
    16561 cgcacattat acgcgtttgc ccggattcaa acgaaatttt tatcccgccc cgccaaaccg
    16621 ccggcggctt cagacggcac ggcaacttga tataccgtct gaacacgcgg ttcagatgcc
    16681 gtccaagtcg ttgaacatca acccgatacc gataccgttc tgcttgtggt tgtagtcgat
    16741 cagactctcg ccgtaaccgt ggaatccgcg taccacgcct ttgagtttgc ccttaatcgg
    16801 aaacgtgtag gcggcttcaa tcgcgccgta gcccgttttg gggttgtagc gcaatacgga
    16861 atacacattc tgcctgtcgt tcaggcggta ctgcagcttc acgtcgccat accccatata
    16921 gtcggcaata tcgggattgt cgtttttatc gccgctctga tcgaacgcac gcacccacac
    16981 gcgcggaatc accgtcagcc ttccccattc catgcctgcc atggcataaa tcctgttcca
    17041 cgaacgcgat tcgggacggc tctgtccgtt ggactggtgg acaaaacctg caccgagcat
    17101 acgcagcctg ccgccgaacg gcaaatctgc cttcacaggc tgggtcagga aaatttcagg
    17161 tttgtaatcc gtattgcgga acggcgcgga tttcctgcct tggttgtaaa tctgccaatc
    17221 ggatctttgg gtgtagccga accacagatc cgcgcgggtt ttaaacaaat cttcggcaat
    17281 tttgcttttg aacgaaacct gcaatttggt ttccgcacgt ttctgctgtc cgaatttttc
    17341 ctgtacagtc gtaccgcgcg tcggcgaacc cggggcatag ttgggcgaat tgttgtacca
    17401 gagcggcata aggtacatcg gattgtgttc gcgtacgccc aacagcccgc gcaaatcgtt
    17461 tttgtccaag tcgtacatca ggctcaaagg cgtatagatg tcggcggttt cgcccgcact
    17521 gtcggcagga agcgcatccc cgcctttttc aacaacaatg accgcctcgc ccttatccag
    17581 gctgctgcgg acggtttccg tcagattgag tacggctttc gactcctgcc cttcctgccc
    17641 tgccgaagac ggaagctgtg ccgcaaaaat cctgtcgtaa cacgccaaac gcgtaacatt
    17701 gtccgtcaaa gcggcgcatt gcagcgcggt ctctccaaaa gcggatgcca tcggcaacag
    17761 tcctgtcaaa agaatatagc gcatattccg tgtattcatc tccgccccca ttgtcggcat
    17821 attggttttc aaacggcatt ttatagcgga ttcggataaa aaccgcaccc tttccgccat
    17881 ttcgggattt tgcccccgca atacagaaaa ccccgaaacc gtcgggcttc agggttttcc
    17941 gcttatcgcg tatcaaccgc cttggcggtt ttgcaaaaat caagccagtg cttttacttt
    18001 tgcagacaga cggcttttgt ggcgtgccgc tttgtttttg tggaacacgc ccttgtcggc
    18061 gatgcggtcg atgactttga cagactcttg gtaaaccgct tgtgcggcag ctttatcgcc
    18121 tgcttcgact gctttcaata cttttttcac tgcagtgcgg aatgcggtac gcaggctagc
    18181 gttgtgggcg cgttgtttga cggactggcg ggcacgtttg cgtgcttgtg cgctgtttgc
    18241 catattggaa tctcctggaa gaaaaataaa ttctgtaaac gcgcaatttt aaagggggtt
    18301 tacattatat gcaagccttt tctctctatc cgcccgtcgg acaccctgac agcgcaaaaa
    18361 aacggcaaaa tcgtgcggac gttttccccc tcttttgaaa acagggtaat tttttaggga
    18421 aatcagtcca atatcacttt gagtttgcac aaatttactt tacaatgccc aaaccttgcg
    18481 ccggggtgtt tatggggcgc ggggttttct ttttttccaa cctctttcat caggaatctg
    18541 cccgtatgaa aaaatccgta ttagccgttt tggccgcgct gtctctggca gcctgcggcg
    18601 gcagcgaaaa aaacgccgtg caacctcaag cagacgctgc gtctgctgcc aatgccgaag
    18661 ccgctgccac cgatactctg aacatctaca actggtcgaa ctatgttgac gagagtacgg
    18721 tcgaagactt taaaaaagcc aacaatctga agctgactta cgacttgtac gaaaacaatg
    18781 aaacgctgga agccaaaatg ctgaccggca aatccggtta cgatttggtt gtgccgggca
    18841 tcgccttcct gccgcgccaa atcgaggcgg gcgcgtatca aaaagtcaac aaagacctga
    18901 ttcccaacta taaaaacatc gatcccgaac tcttgaaaat gctggaaacc gccgacccgg
    18961 gcaaccagta tgccgtcccc tatttctccg gcgtgaacac gattgcgatt acggcgaagg
    19021 gcaaagagct tttgggcggc aagctgcccg aaaacggctg ggatttgctg ttcaaacccg
    19081 aatacaccca caagctgaaa tcctgcggca tcgccctgtg ggacaccccg agcgaaatgt
    19141 tcccgatttt gctgaactac ttgggcaaag accccaaagg ttcgaatcct gaagatttga
    19201 aggcggcggc ggaagtgttg aagtctatcc gtccggatgt caaacgtttc agcccgtcca
    19261 tcatcgacga gctggcgcgc ggcgacatct gcctggcggc aggcaacggc ggcgatttga
    19321 acttggcgaa agcacgttcc gaagaagtga aaaacaacgt cggcatcgaa gtgctgaatc
    19381 cgaaaggcat gggcttctgg attgagtctt ggctgattcc cgccgatgcg aaaaacgtcg
    19441 ccaatgccca caaatacatc aactacacgc tcgaccccga aatcgcggcg aaaaacggca
    19501 tcgccgtaac cttcgccccc gccagcaaac cggcgcgcga aaaaatgcct gccgagctgg
    19561 tgaacacccg ttccatcttc ccgaacgagc aggatatgaa agacggtttt gtgatgccgc
    19621 aaatgagcac ggatgcgaaa aaactgtctg tcagcctgtg gcagaaaatc aaagtcggca
    19681 ccaactgatt tgaagcatta aaaatgccgt ccgaacatcg ttcggacggc attttatatt
    19741 ggattgaaat agaaatattt atatagtgga ttaacaaaaa tcagaacaag gcgacaaagc
    19801 cgcagacagt acaaatagta cgaaaccgat tcacttggtg cttcagcacc ttagagaatc
    19861 gttctctttg agctaaagcg aggcaacgcc gtactggttt ttgttaatcc actataccgt
    19921 cgttcccacg gtcaggattt cagattgcgg acatctgtca gaaaagacaa aaaaccttcc
    19981 gccgtcattc cctacaggcg agaatccgat ccgttgaaat tcggttgttt taaataaatt
    20041 cttgcagctt tgattttctg tttttccgat aacgccgtaa ctttgaaacg cgaaagcggt
    20101 aatccgatcc gttcggtttc ggtttttttg aggtttcggg taacttctaa accgtcattc
    20161 ccacgaaaac agaaaatcaa aaacagaaac ctcaaatccc gtcattccca cgaacctgca
    20221 t
//
LOCUS       DAWFJW010000026        19910 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFJW010000026 DAWFJW010000000
VERSION     DAWFJW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.026"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..380)
                     /locus_tag="WGR19_000772"
     CDS             complement(<1..380)
                     /locus_tag="WGR19_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798331.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WGR19_000773"
     CDS             complement(438..803)
                     /locus_tag="WGR19_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798332.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WGR19_000774"
     CDS             complement(785..1330)
                     /locus_tag="WGR19_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798333.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WGR19_000775"
     CDS             complement(1493..1765)
                     /locus_tag="WGR19_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798334.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYVIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(1834..2268)
                     /locus_tag="WGR19_000776"
     CDS             complement(1834..2268)
                     /locus_tag="WGR19_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798335.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNREAHWTAIGGGALIG
                     AMNPINGGRALINGMRGVAVGTVAGGINGYAGSTGKNTDIRR"
     gene            complement(2311..3180)
                     /locus_tag="WGR19_000777"
     CDS             complement(2311..3180)
                     /locus_tag="WGR19_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0798336.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            3344..4267
                     /locus_tag="WGR19_000778"
     CDS             3344..4267
                     /locus_tag="WGR19_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0798337.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSRFCL"
     gene            complement(4345..4986)
                     /locus_tag="WGR19_000779"
     CDS             complement(4345..4986)
                     /locus_tag="WGR19_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0798338.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVSARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGRGETQDCLAVIDYACAQHPE
                     APEFALSGFSFGGYVATFAAQARTPDLLLLIGAAVCHYTDRPEPSAVPDVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(5041..5832)
                     /locus_tag="WGR19_000780"
     CDS             complement(5041..5832)
                     /locus_tag="WGR19_000780"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0798339.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFESEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFYL"
     gene            5998..6073
                     /locus_tag="WGR19_000781"
     tRNA            5998..6073
                     /locus_tag="WGR19_000781"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6031..6033,aa:Thr,seq:tgt)
     gene            6108..6183
                     /locus_tag="WGR19_000782"
     tRNA            6108..6183
                     /locus_tag="WGR19_000782"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6141..6143,aa:Thr,seq:tgt)
     gene            6325..7116
                     /gene="panB"
                     /locus_tag="WGR19_000783"
     CDS             6325..7116
                     /gene="panB"
                     /locus_tag="WGR19_000783"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0798340.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVACPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMHGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            7106..7282
                     /locus_tag="WGR19_000784"
     CDS             7106..7282
                     /locus_tag="WGR19_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798341.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            7239..8075
                     /locus_tag="WGR19_000785"
     CDS             7239..8075
                     /locus_tag="WGR19_000785"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0798342.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            8240..10078
                     /locus_tag="WGR19_000786"
     CDS             8240..10078
                     /locus_tag="WGR19_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798343.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVVKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPVGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            10218..10799
                     /locus_tag="WGR19_000787"
     CDS             10218..10799
                     /locus_tag="WGR19_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0798344.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGIPSETETPERCAARTR"
     gene            10809..11654
                     /gene="ispE"
                     /locus_tag="WGR19_000788"
     CDS             10809..11654
                     /gene="ispE"
                     /locus_tag="WGR19_000788"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0798345.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            11662..11737
                     /locus_tag="WGR19_000789"
     tRNA            11662..11737
                     /locus_tag="WGR19_000789"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11695..11697,aa:Gln,seq:ttg)
     gene            11750..11825
                     /locus_tag="WGR19_000790"
     tRNA            11750..11825
                     /locus_tag="WGR19_000790"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11783..11785,aa:Gln,seq:ttg)
     gene            11834..11909
                     /locus_tag="WGR19_000791"
     tRNA            11834..11909
                     /locus_tag="WGR19_000791"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11867..11869,aa:Gln,seq:ttg)
     gene            12076..13059
                     /locus_tag="WGR19_000792"
     CDS             12076..13059
                     /locus_tag="WGR19_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0798346.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            13126..13698
                     /locus_tag="WGR19_000793"
     CDS             13126..13698
                     /locus_tag="WGR19_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0798347.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(13823..14992)
                     /locus_tag="WGR19_000794"
     CDS             complement(13823..14992)
                     /locus_tag="WGR19_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0798348.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWARLTGQ"
     gene            15140..16666
                     /gene="ilvA"
                     /locus_tag="WGR19_000795"
     CDS             15140..16666
                     /gene="ilvA"
                     /locus_tag="WGR19_000795"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0798349.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(16722..17795)
                     /locus_tag="WGR19_000796"
     CDS             complement(16722..17795)
                     /locus_tag="WGR19_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0798350.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENTDSGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNIAFGLTVL
                     PKPERPSKEQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAEG
                     KELQLIPKQVYVFSQNELIEYSI"
     gene            complement(17792..18652)
                     /gene="cysW"
                     /locus_tag="WGR19_000797"
     CDS             complement(17792..18652)
                     /gene="cysW"
                     /locus_tag="WGR19_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0798351.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(18907..19743)
                     /gene="cysT"
                     /locus_tag="WGR19_000798"
     CDS             complement(18907..19743)
                     /gene="cysT"
                     /locus_tag="WGR19_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0798352.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT         4788 a         4680 c         5432 g         5010 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac ctccatccga aacagaaacc aatatatttt
     1621 taattaatac atagctgatt attacattta ttattcctat aacatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agtgctcttc ctccgttaat agggttcatc gcaccaatta acgcaccacc
     1981 tccaattgct gtccaatgtg cctctcgatt atacgagcca ctagcaatag cactagccat
     2041 atagctgaaa tgccctccaa ctcctcccat aatgcctcca acagcattag gaatccactc
     2101 cccctcagtc tccttcatct ccttttgaga aagctccgcc aactgcatcg gcgcatctgc
     2161 ctgcgtgtgg aacacttggt tttcaaatgt ctgattgtcc aagccgtttg ccattgcggg
     2221 ggcaatcata gacagcatca ttacggctga ggtgatttgt tttttcataa taactccttt
     2281 ggattacaag gttggaaaat caaaacctgc ttaaaatgta tgctgtacgc caaatttcag
     2341 ttcggaactg ctttgccctg aaacgttgaa acgtgcggat gcgtttaaag ccgtggtttt
     2401 ggtgaaaccg aaacctgtgc cgaaatgagc gtaggtggat gtgtttcttg cggtttcttt
     2461 tttgccgtct atgcggtcgg gctgtgtgcc cagccattgg atgcctccgg tcaggctgat
     2521 tctgtcgttg gcggcaaatg agatgttggg gttgagcagc aggtagttgc ccgatttgta
     2581 gcggatgccg tctgaaaggg ttttgctgcc gttgatgcgg taggcggcgg tgagggaaag
     2641 gacaatcgga tctatggctt tgtaggtggt ggcgccgatg agccaggatt ttcccgacga
     2701 ggctttgttg cgcgattttt cgtaaaccgt gctttcaaga aagctgatta gggcggggtt
     2761 tttgtcgtct ttaaggaaag tgtggctgat gccgagggat acgtcggaca tccgtttgtt
     2821 gcgggttttg ccgttgccgt cgagtttgcg ttcttcgtgc cacagatagc tgccgctgcc
     2881 gtaaatgtcg gtattcccgg tcagtccgta gcgcaaaccg agcgtgccaa cgagcatatc
     2941 ggtattgctg ccgttttctt ggatttcggt cggaatgggg ataaacgagg ttgcgccggt
     3001 ttggatgtaa accggtgcgg caagttcggc gcggttgttt tcgctgttca ggtaggtaag
     3061 ggaagtttcc agtttccatt tgcccttgtc ggtcattatg tcttcaatcg tcaagggcag
     3121 gtcggcataa gcggataaag gcaggatggc gggcaaggcg ggcaaaaaga tgcgcttcat
     3181 atttctccta ttgatattaa ttcttattta ctgataataa tgaatatccg ataatcttgt
     3241 ctaaataaat ttttaaatca taaaaaacaa tatgtttgtt tttatcaatt atgttttgat
     3301 atgccgtctg aaaagtttgt gaaaaacggt tacaatccgc cgcatgaaaa aacgcaataa
     3361 tcctcttccg ctgttgaacg gtgtcaaacc cagttatttg gtgctgccgc atgaaaagca
     3421 gttttacggg ctgccgctgc tgcattttct gtgcatccgc tttccttttg tgggcgcgga
     3481 cgattggcgc aggcggttga acagcggttt tgtggtcggt tcggatggtg cggcgttgga
     3541 cgaacattct ttgttcgagc cgggtaagac gatgttttat tatcgggaaa ccagcagtga
     3601 ggatgagccg cgtattccgt ttgaagaaaa gattttgcat attgatgagc atttgattgt
     3661 ggtggacaag ccgcattttc tgcccgtcat ccccagcggg cgttttttga gggaaacgct
     3721 gctcacacgt ctgcgcctgc gtcctgaatt gcagcatttg aatgttgagg acattacgcc
     3781 gctgcaccgc ttggacaagg atacggcagg cgtgatgctg ctgtcgcata atcctgccac
     3841 gcgcggggct tatcagacga tgtttcaaaa caaaacggta tggaaaacgt atgaggcact
     3901 tgcgccgaca aggacggatt tgccgtatcc gctcgatgtg gtttcgcgtt tggtgagggg
     3961 tgagaaattt tttacgacgc aagaggcgga aggcgaacca aacgcacata cgacggtcga
     4021 actgattgaa aacagggggg aattcagcct ttaccgcctt acgccgcata ccggcaagaa
     4081 acaccagttg cgcgtgcata tgatgggttt gggtatgccg ctgttgaatg acgcgctcta
     4141 tcccgtgccg tctgaagcgg gcagcgagga ttatcggaaa cctttgaagt tattggcaaa
     4201 aaagattgcg tttgcagatc ctttgtcggg tcgggaaagg gtgttttgca gtaggttttg
     4261 cctataatga gagggaatgc aaacccgtat cggcagatgc cgccgatacg ggtttgatat
     4321 tatcgatgcc gtctgaaagg ggttttatcc gttcaggcat acgggcgcga agcggaggat
     4381 ggtgtcgcgc aggacgatga gtttgccgtg aaagaaatgc gtggaaccgg cgatggtaat
     4441 gacgggcaaa tcttgcggtt ctgcccattt caatgctttc ccgatttcga cgacttcatc
     4501 ttccgcgccg tgtatcatca gcgtttttgc aacgtcggga acggctgacg gttccggacg
     4561 gtcggtatag tggcagactg ccgcgccgat gagcagcaat aaatcgggtg tgcgcgcttg
     4621 tgcggcaaat gtggcgacat aaccgccgaa ggagaagccg gataaggcaa attcgggggc
     4681 ttcgggatgt tgggcgcagg cataatcgat gacggcgagg cagtcttgcg tttcgccgcg
     4741 tccgtaatcg tgtgtgcctt cgctgttgcc tacgccgcga aggttgggca ggtagcagtg
     4801 gaagccgagt ttgcttaagg ctttggcggc agtttggatg actttgttgg tgtttgtccc
     4861 gccttgtagg gggttgggat gattgatgac ggcaacaccg cgtgccgata cttgttcgga
     4921 cgggatatgg atggtttcta aaatgccggc aggaccggat atgtgtatgg tttcgggttt
     4981 cagcataatg atttcctctt gattttccgc atgttccaat gcggtcatga agatagttgc
     5041 ttaaaggtag aaatgcttgc cgttgttggg gttggacgct ttcaggccgg caagcatgcg
     5101 gtggaagtcc aatccgtatg cgttgctcag tttgtcggca cgttttttga gtttgtctat
     5161 gttttgctct ttggggctgc tttggaaggc cattaccgcg acattgccgt ggctttcggc
     5221 aggaagttcc aatacgcgcc cttcaaaaac gctcaacaac cgttcgatga agcgttgata
     5281 gcgtttgtcg ccactccacc agttggtaac gaatatgccg tctgaagaga gtgcgtttcg
     5341 gcagtctcgg aagaacggtt cttcaaccag cgtatcgatg atttgttcgc cgtcgaatcc
     5401 gtccaccaag atgatatcag tgttgtggcg gaagactttg atgtactctg caccatccgc
     5461 ttcaataatt tcaaatttct cactctcgaa aggcaactca aacaggttgc gggcaatggc
     5521 gataacctgc gggttgatat ctacggcagt ttgacgcgtg tcgggcaggt aggtgtctat
     5581 ccagcgcgca aacgagccgc cgcccaagcc gatttgggtg atatgctgcg gaagcgcgtc
     5641 ggtaaacagc agccatccca tcatcgcgcg gctgtaagag agtaccagct cggacgggtg
     5701 gtcgaggttc attgagcttt gaacggtgtc gctgcccaag tgaagcgaac ggatattgcc
     5761 ttcttcggaa atgccgactt cgggaaagcc ggattttgca ggacgcaggc ggcggtaggg
     5821 atgtcttgcc atcggtgtga acggtcggtt tgaaaggcgg atattttatc agaatgccga
     5881 tgcttgtcct gcttcaaatt aatttctttt caaataaatt acttattcgg atttgccggg
     5941 gctttcggat aaattccttg ccaaagtgtg aagttgcctg cataattcgc cttctttgcc
     6001 ggtatagctc agttggtaga gcacctgact tgtaatcagg gggtcccgag ttcgactctt
     6061 ggtgccggca ccatttctgc tgatggtgtt gcaaaacatt tcagtaagcc ggtatagctc
     6121 agttggtaga gcacctgact tgtaatcagg gggtcccgag ttcgactctt ggtgccggca
     6181 ccagatttga cagtccgatt gtgtaaaaca gtcgggctgt tttgcatttg cggcacggtc
     6241 ggtggcaggg ctgcgggcat ttcactataa tagcccgttt caaactgaaa aggccgtctg
     6301 aaaagggcgg ggtaacaata tctgatgatt actgtgaaca cactgcaaaa aatgaaggcg
     6361 gcgggcgaga aaatcgctat gctgaccgct tacgaatcca gttttgccgc actgatggac
     6421 gatgccggcg tggaaatgct gctggtcggc gactctttgg gtatggcggt tcaagggcgg
     6481 aaatcgacgc tgcccgtcag cctgcgcgat atgtgctatc acaccgaatg tgtagcacgc
     6541 ggtgcaaaaa atgcgatgat tgtcagcgat ttgccgtttg gtgcatatca gcagagtaag
     6601 gagcaggcgt ttgccgccgc cgccgaactg atggcggcag gagcgcatat ggtcaaactc
     6661 gaaggcggcg tgtggatggc agaaacgact gaatttctgc aaatgcgcgg tattccggtt
     6721 tgcgcgcaca tcggtctgac gccgcaatcc gtgtttgcct ttggcggata taaggttcag
     6781 gggcgcggcg gcaaggcgca ggctttgctt aacgatgcca aggcgcacga tgatgcgggg
     6841 gcggcggttg tattgatgga gtgtgttcct gcggaactgg caaaaaaggt aactgaaact
     6901 gttgcctgtc cgaccatcgg catcggcgcg ggggtcgatt gcgacgggca ggttttggtg
     6961 atgcacgata tgctcggtat ttttccgggc aagacggcga aattcgtcaa aaactttatg
     7021 cacgggcaaa gcagcattca ggcggcggtt cgggcgtatg ttgccgaagt caaagccaaa
     7081 accttccctg ctgcggaaca tatttttgca gactgaacaa ggtttgctgc tgatgccgtc
     7141 tgaaagccgt ttcagacggc attttgtttt gccgtgcgcg gcgcgtataa tcggcgcgtt
     7201 ttgtcgggca ggaagcccga aggataagga ttaccgttat gcaaatcata cataccattc
     7261 gagaactgcg cgcgtggcgt aaaaatgcgg gaaaggtggc atttgtgccg accatgggca
     7321 atctgcatga agggcatctt gcgcttgtgc gtgaggcgaa aaaacgcgcg gacagtgtcg
     7381 tggtcagcat tttcgtcaac cgcctgcaat tcggtcaggg cgaggatttc gacaaatatc
     7441 cgcgtacttt gcaacaggat gcggacaagc tggaagccga gggtgttgcc gttgttttcg
     7501 cgcccgatga gaaagaactc tatccgaacg tggagcaacg gtataacgtc gaaccgccca
     7561 acctgcaaaa tgagttgtgc ggcaagtttc gtccgggaca tttccgcggg gtggcaacgg
     7621 tagtgtccaa gctgttcaat attgtcgcgc cggatgttgc ctgtttcggc aaaaaagact
     7681 atcagcagct tgccgtgatt aaaggtttga ccgaggattt gaattttgat gttgaaatag
     7741 tgcctgtcga tacggggcgc gcggaagacg gtttggcact ctccagccgc aaccagtatt
     7801 tgagtgcggc ggaacgcgac gaagcaccgc gcctgtaccg cgaattaaag gctgttgccg
     7861 aatccttggc gcagggcagt ttggattatg caggtttgga aaaacgtgcc gtccaatccc
     7921 tgacagaata cggctgggtg gtcgattatg tcgaaatccg ccgcgtcgat acgctcgaag
     7981 tggcgcgggc gggagataag aaactggtgg tcttggccgc cgcccgtctg gggacgacgc
     8041 gcctgattga caatttggaa ataaaactcc cttaaaccgc aagcgtcggg aatgccgtct
     8101 gaagcggatt tgcgtttcag acggcattta tttttttgaa cggggtttcg caaatctaca
     8161 aacgattctg cttgtgataa agttacgcct gattatatgc cgtctgaagg ttcggacggc
     8221 tgtcggataa aggaagatta tgttacccgc ccgtttcacc attttatctg tgctcgcggc
     8281 agccctgctt gccgggcagg cgtatgccgc cggcgcggcg gatgcgaagc cgccgaagga
     8341 agtcgtaaag gttttcagaa agcagcagcg ttacagcgag gaagaaatca aaaacgaacg
     8401 cgcacggctt gcggcagtgg gcgagcgggt taatcagata tttacgttgc tgggagggga
     8461 aaccgccttg caaaaggggc aggcgggaac ggctttggca acctatatgc tgatgttgga
     8521 acgcacaaaa tcccccgaag tcgccgaacg cgccttggaa atggccgtgt cgctgaacgc
     8581 gtttgaacag gcggaaatga tttatcagaa atggcggcag attgagccta taccgggtaa
     8641 ggcgcaaaaa cgggcggggt ggctgcggaa cgtgctgagg gaaagaggaa atcagcatct
     8701 ggacggactg gaagaagtgc tggctcaggc ggacgaagga cagaaccgca gggtgttttt
     8761 attgttggca caagccgccg tgcaacagga cgggttggcg caaaaagcat cgaaagcggt
     8821 tcgccgcgcg gcgttgaaat atgaacatct gcccgaagcg gcggttgccg atgtggtgtt
     8881 cagcgtacag ggacgcgaaa aggaaaaggc aatcggagct ttgcagcgtt tggcgaagct
     8941 cgatacggaa atattgcccc ccactttaat gacgttgcgt ctgactgcac gcaaatatcc
     9001 cgaaatactc gacggctttt tcgagcagac agacacccaa aacctttcgg ccgtctggca
     9061 ggaaatggaa attatgaatc tggtttccct gcacaggctg gatgatgcct atgcgcgttt
     9121 gaacgtgctg ttggaacgca atccgaatgc agacctgtat attcaggcag cgatattggc
     9181 ggcaaaccga aaagaaggtg cttccgttat cgacggctac gccgaaaagg catacggcag
     9241 ggggacgggg gaacagcggg gcagggcggc aatgacggcg gcgatgatat atgccgacag
     9301 aagggattac accaaagtca ggcagtggct gaaaaaagtg tccgcgccgg aatacctgtt
     9361 cgacaaaggt gtgctggcgg ctgcggcggc tgtcgagttg gacggcggca gggcggcttt
     9421 gcggcagatc ggcagggtgc ggaaacttcc cgaacagcag gggcggtatt ttacggcaga
     9481 caatttgtcc aaaatacaga tgctcgccct gtcgaagctg cccgacaaac gggaggcttt
     9541 gagggggttg gacaagatta tcgaaaaacc gcctgtcggc agcaatacag agttacaggc
     9601 agaggcattg gtacagcggt cagttgttta cgaccggctt ggcaagcgga aaaaaatgat
     9661 ttcagatctt gaaagggcgt tcaggcttgc acccgataac gctcagatta tgaataatct
     9721 gggctacagc ctgctttccg attccaaacg tttggacgaa ggcttcgccc tgcttcagac
     9781 ggcataccaa atcaacccgg acgataccgc tgtcaacgac agcataggct gggcgtatta
     9841 cctgaaaggc gacgcggaaa gcgcgctgcc gtatctgcgg tattcgtttg aaaacgaccc
     9901 cgagcccgaa gttgccgccc atttgggcga agtgttgtgg gcattgggcg aacgcgatca
     9961 ggcggttgac gtatggacgc aggcggcaca ccttacggga gacaagaaaa tatggcggga
    10021 aacgctcaaa cgtcacggca tcgcattgcc ccaaccttcc cgaaaacctc ggaaataatg
    10081 caggtccatc ctttcagacg gcataaggtt tgccgggaag ccggggcatt cgggcaaacg
    10141 gcacgcagtt cgcacgcgtt ttgcacggca cgccgaaccc gccggccggc aggatggcac
    10201 ccgttaagga aattctgatg aaacacaccg tatccgcatc ggtcatcctg cttttgaccg
    10261 cttgcgcgca attacctcaa aataacgaaa acctgtggca gccgtccgaa cacatcagca
    10321 gttttgcagc agaagggcgg ttggcagtga aagcggaagg gaaaggttcg tatgcaaatt
    10381 tcgattggac ataccaaccg cccgtggaaa ccatcaatat caatacccct ttgggcagta
    10441 cgctcgggca gttgtgtcaa gacagggacg gcgcattggc agtggacggc aaaggaaatg
    10501 tctatcaggc ggaaagtgcg gaagaattga gcaggcagct ggtcggtttc aaactgccaa
    10561 tccaatatct gcatatctgg gcagatggca ggcgtgtggc gggcgcgcct taccgcatcc
    10621 tgccggacgg catattggaa caatacggtt ggactgtcgg cagaaccgcc gacagtgggg
    10681 ggcaagtccg aacgttgcaa ctgaataacg gaaatttgaa catcaggctg gttttcaccg
    10741 aaatcggtat tccgtctgaa actgaaaccc cggaacgctg tgcggcgcgc acgagataag
    10801 gcggacagat gaatattgcg gacggacggc aggcgtttcc cgcacctgca aaactgaatc
    10861 tcgatttgag gattaccggc aggcgggaag acggttatca caatatcgaa agcatattct
    10921 gcttgataga tttgcaggat accgtatatt tgaaaccgag ggacgacggc aaaatcatcc
    10981 tgcacaatcc ggttgatggc atgccgcagg aagccgattt gagctaccgt gccgcatcgt
    11041 tgctgcaaaa atatgcgcgc aaccccgccg gcgtggaaat atggctggac aaaaaaatcc
    11101 cgacaggggc gggtttgggc ggcggaagct cggatgcggc aaccgttttg ctggtgttga
    11161 accgttggtg gcagtgttcc cgaacgacgc agcagctgat tgacttgggc gcaactttgg
    11221 gtgcggacgt accatttttt attttcggaa aaaatgcttt tgcaagcggg ataggtgaaa
    11281 aactgatagg aatggatatt ccgaaacagt ggtatgtcat cgtcaaaccg cccgtccacg
    11341 tttccactgc aaaaattttc acacacgaag gcttgacacg aaattccgcc tcaagcataa
    11401 tgccgacttt ccaaaacctg caaccgttta gaaatgatat gcaggcagtt gtatttaaag
    11461 aataccctga agtttggaaa gcctattccg aattgtcccg atatggattt gccttaatga
    11521 caggttctgg tgcgtgtgta ttcacggcgt gtcaagatag ggatagcgca tacaatatat
    11581 accgacaagt ttcaggtttg tacgaggcat atttggcaga gggtctttca aaacatcctt
    11641 tgttgtccgt ataaacattg ttggggagtc gtcaagcggt taagacactg gattttgatt
    11701 ccagcatgcg aaggttcgaa tccttcctcc ccagccaagc caaacgagtt ggggagtcgt
    11761 caagcggtta agacactgga ttttgattcc agcatgcgaa ggttcgaatc cttcctcccc
    11821 agccagccct ttttggggag tcgtcaagcg gttaagacac tggattttga ttccagcatg
    11881 cgaaggttcg aatccttcct ccccagccaa ataaaagcgt gtaagcctgc ttacacgctt
    11941 ttatttcata gaaataaaaa tattgaaatg cctttgtttg tgtcggatgt tgcaggtata
    12001 atgtcgggct tggtacaagc agagggaagc attgtgtttt ctgagcggaa gttaaacata
    12061 aaatcaggtg agaatatggc tgcgtacgac agtttgatgg tatttacagg caatgccaat
    12121 cccgaattgg cacaacgtgt tgtcaggcat ttggacattt ctttgggcaa tgcttccgta
    12181 tccaagtttt cagacggcga agttgccgtc gaactgttgg aaaacgtacg cgggcgcgat
    12241 gttttcatcc ttcagccgac ctgtgcgccg accaatgaca acctgatgga aatcctgacg
    12301 atggcggatg cactgaagcg tgcttcggca ggtcgtatta ccacagccat tccgtatttc
    12361 ggctatgcgc gccaagaccg ccgtccgcgt tccgtccgcg ttccgatttc tgccaaactg
    12421 gtggcaaata tgctgtattc ggcagggatc gaccgtgttt tgactgtcga tttgcatgcc
    12481 gaccagattc aaggtttctt cgatattccg gtggacaata tttatgccac cccgattctg
    12541 ttgaacgaca tcaaacaaca gcggattgaa aatctgaccg tcgtcagccc ggacatcggc
    12601 ggtgtcgtcc gcgcccgcgc cgtggcaaaa tccctgaatg ccgacttggc aatcatcgac
    12661 aaacgccgcc cgaaagccaa tgtggcggaa gtcatgaaca tcatcggcga tattcaaggt
    12721 agaacctgtc tgattgtgga cgatatgatt gacactgcaa atacgctgtg caaagccgcc
    12781 gtcgccctga aagagcgggg ggctgaacgt gttctagcct atgccagcca cgccgtattc
    12841 tccggagagg cggtcagccg tatcgcctca tccgaaatcg accaggtggt cgtaaccgat
    12901 accattcctt tgtctgaagc ggctaaaaac tgcgaccgta tccgtcaggt aacgattgcc
    12961 ggtctgttgg ccgaaaccgt ccgccgcatt agcaatgaag aatccgtctc atatcttttc
    13021 aatgaagaag tgatgacagg cagcatgttg ctgccataag cccgaagccg tcttaagctg
    13081 gtcgcggccg atgacggcga ttttacctaa cttggagtat ttaacatgac ttatgaaatt
    13141 caagcctctg ttcgtgaagc acaaggcact ggtgcgagcc gccgcctgcg tcgcgaaggc
    13201 caaatccccg gcattctgta cggtgaaggt caagagcctg ttgcaatcgc tgtggatcac
    13261 aaaaccgtat tctacgcatt ggaaaaagaa tctttccata ctgcgttgat taagttgtct
    13321 ctgaacggtg aaaccaaaga cgttatcgtc cgcgatttcc aaatgcaccc gttccgccgc
    13381 gaagttcaac acatcgactt ccaagctgtg aaagcagacc aacccgtacg catccgcgtt
    13441 cccctgcaca tcgttaacgc tgaaaattcc caagcggtca aactgcaagg cggccgcgta
    13501 tctctgttaa acacttctgt tgaagtagtt gctttgcctg ccaacatccc tgctttcttg
    13561 gatttggatt gtgctgaagt ggttgccggc gacattctgc acttgtcaga catcaaactg
    13621 cctgaaggtg tagaaagcgt ttccctgaaa cgtaacgaaa atctggctgt tgctaccgtt
    13681 accggtaaga aacgctaatt gatttcagca gcagggcggc gcgtatgcaa tacgtaccgc
    13741 cctgttgttt tatgccgtct gaaccgtgtt tcagacggca tttctttatt tgttggaaaa
    13801 acgggatatt tgaaatagca gattactgcc ctgtcagacg cgcccaaagc ctttgccacc
    13861 ggcttctttt ttttacattt tccagtgcga cgatttcttt ttcggcaatg gtgtatccgt
    13921 tttgtctgat tttgattttt cctaaaattt gccctttttt taccggggcg ggaatcggct
    13981 gtatggtttc caaaatctgt tctgccattt tcgcttcctt atgtggcaga gtgatgtagg
    14041 cttctttgag gaagcctgcg cggacggttt ttttgctgcc tccggaaatt tggatttggg
    14101 caacggtttt gcctttcgga tatattttgg gcgtatcgaa ggcctgcaat gcccagttca
    14161 gcagcttgct gttgtctgat gcgcgtgttt ccgccgattc cgaacccaat gtgatgacaa
    14221 ggatgtgcct gccgttgccg gagtatgaca cggcaaggtt gtagccgccg ctttctgtgt
    14281 gtccggcttt cagaccgttt acattgttgt ccctatataa aaggatattg cggttgtttt
    14341 gctctatatt tttgaatttg aaagatttga tggaaaacag cgggtaatat tccggaaagt
    14401 cgcgcatcaa tgcttcagac agctgggcga ggtctttggc ggtggaaacc tgtccttctc
    14461 tactcaagcc tgtcggattt ttgaatacag tgttcttcat gcccaagcgt cgggcttctt
    14521 tgttcatttg ttgcacaaaa ttttcaatcg agccgttgcc cagccggccg gcaagggtta
    14581 gggcggcatc gtttgcggat agtgcaatca tgccttttaa gagtttgtcg gtgctgaccg
    14641 tatcgccggg acgtacaaac attctgcttc cttctgaagc ccatgcggat tcgggtattt
    14701 ttaagttttc ttcagatcgg atatttcccg atttcatgtt tttgaaaacc agatatgcgg
    14761 tcatcagttg ggttagtgcc gccggttcga caggggtatt gatgtttttg gcggataaaa
    14821 tctgtttgct ttgaaggtcg ataacgatgt gtgccgctgt gagggtttcg ggtgtttgga
    14881 acgtgggggc ggcgtgtacc gccggtttgt tgggcgcggg cgatgcggcc gttgcgtgag
    14941 aaacgcctaa gatgatggaa agcaggacgg gcaggatttt atgtgctgtc atgaaatatt
    15001 ctaattgtgt gcgtgtttca gtctgccgat tatacgctta gggtgtctga tcgggcggat
    15061 ttttcttgct ttcgcgccgt cttgggcgta tggttttggg ttttgcgatt ttaataaacc
    15121 gattatccca tattgaatta tgaacacgcc ccttccttat tccaattacc tcatccgcat
    15181 cctgacggca tctgtctatg atgtcgcggt cgaaacgcct ttggaaccgg cacgcagcct
    15241 ttctgtacgt ttgaaaaaca acatcctttt gaaacgcgaa gatttgcagc cggttttttc
    15301 gttcaaaata cgcggcgcgt acaacaaaat gtccaagttg ccgaaagatg cgctcgcttg
    15361 cggcgtgatt gcggcaagcg cgggcaatca tgctcaaggc gtggcattgt ccgcacagcg
    15421 tttgggttgc cgtgccgtta tcgttatgcc ggaaactacg ccgaaaatca aagtggatgc
    15481 ggttaaaagc catggcggcg aggtggtttt gcggggcgtt tcatacaacg atgcctacga
    15541 ttatgcgatg gagttggcgg aaaaagaagg gttaacctat atcgcgccgt ttgatgatcc
    15601 tgatgtgatt gcaggacaag ggacggtagg gatggaaatt gtcagccagc atcccgatcc
    15661 aatccgcgcc gtattcgtac cgataggcgg tggcggtttg gcggcgggcg tggcggcatt
    15721 tatcaagcag gtccgtcccg aaatcaaagt tatcggcgtt cagaccaacg attcctgctg
    15781 tatgaagcag tcggtcgaag cgggtgaaat cgtccatttg aaagatgtcg ggctgttttc
    15841 agacggcact gcggtcaaag tcgtcggaaa cgaaaccttc cgtctctgca aagaactttt
    15901 ggatgaaatc attacagtcg ataccgatgc ggtttgcggc gcggtcaagg atattttcga
    15961 tgacacgcgc agcattaccg agccggcggg cgcgttggcg ttggcgggtc tgaaagccta
    16021 tatcgcccga gaaggcgcgg aaaaccaaac cctgattgcc gttaccagcg gtgcgaatat
    16081 gaattttcac cgtttgcgcc acgtttcgga acggagcgaa ttgggcgagg gcaacgaagg
    16141 tatttttgcc gttaccatcc ctgaagaacg cggcagcttc cttaagtttg tcaatatatt
    16201 gggaaatagg aatattaccg agttcaacta ccgctacggg gacgatgaaa aagcgcatat
    16261 ctttgtcgga cttcaagcgg caggcccgca ggatttggcg gttatcggca gccggttgga
    16321 tgaggcggga ttgcccaatg tcgacctgac tgatgatgag attgccaaaa tccatatccg
    16381 ttatatggtt ggagggcgga cggacaaagt agaaaacgag cgtttggtca gttttgagtt
    16441 tccggagcgt ccgggggcat tggcacgctt tttgaaccat atgcagggag ggtggaatat
    16501 tacgctcttc cattaccgca accacggtgc ggattacggg cggattttgg tcggtatcga
    16561 cgtgccgccg cacgatgccg ccgcatttga cggtttcttg gaaagtctgg gatacagcta
    16621 tcacgaggaa acgcaaaatg ccgcgtacaa gctgtttctt gcttaatggg taatacacaa
    16681 tgccgtctga aagtctttca gacggcattg cgttttcagg atcaaatcga atattcaatc
    16741 agctcgtttt gagaaaagac ataaacctgt ttcggtatca gctgcaattc cttgccttcg
    16801 gcgatcgggt aacgcgcggc atcgctgccc gccagtgtga tatgcacgtc ttgtttgccg
    16861 tgtttcacca aaacgtgcgt caatgcgccg acggcgtgga ttttttcgat ttcggcacaa
    16921 atcatcgggg tttcgtgttc ggcggcgatc tgccattcgt gcgggcggat atagccggtg
    16981 gcggtttgtt cctgccattt gtattgcgcg tccaatttcc acgcgaagcc gttgtaatgc
    17041 cagaagcctt tttcgatgcg tccttcaaat gcgtcggttt cgcctaagaa ctcggtaacg
    17101 aaggcgtttt cgggtttgcg gtaaatggct tcggcactgc cggtttgttc gattttgccg
    17161 tggttcatca cgacgatttc gtcggaaact tcgagggctt cttcttggtc gtgcgtaacc
    17221 agaatgctgg ttacaccgag gttgtgatga atgtcgcgca gccaggtgcg taattctttg
    17281 cgtactttgg catccaacgc gccgaagggt tcgtccagga gtaggagttt gggttcgacg
    17341 gcgagggcgc gggcgagggc aatgcgttgg cgttggccgc cggagagctg gtgcggatag
    17401 gattttgcca aatgcgagag ctgcacgagc ttgagcaatt cttcgacttt ggcgcggatt
    17461 tgttctttgg acgggcgttc tggcttgggc aatacggtca aaccgaaggc gatgttgtca
    17521 aacacgttca tatggcggaa aagggcgtag tgttggaaca cgaaaccgac tttgcgctcg
    17581 cgcacatgtt tggcggttac gtcttgcccg tcaaacagaa tcttgccgct gtcggtgttt
    17641 tccagtccgg cgataatgcg taaaagcgtg gttttgccgc agccggaagg tccgagcagg
    17701 gaaacgagtt tgccggtggg gacgttgagg ttgatgtttt tcagcgcatg aaaattaccg
    17761 aagtgtttgt ttaggttttg gatggtgata ctcatactgc gttcctttcg gcggcggcga
    17821 gttttttgtc ttgtaatttg gtaatgatgt tctgcaccgc cagcgtcgcc agtgccaaaa
    17881 gtgccaatac gccggagagg gcgaatgcgc cggtgaagtt gtattcgttg tagaagattt
    17941 cgaccaaaag cgggacggtg ttggtttcgc cgcgtatgtg tcccgatacc acgctgaccg
    18001 cgccgaactc gcccatcgcg cgggcgttgg tgaggatgat gccgtagagt aacgcccatt
    18061 tgatgttggg cagggtaacg cgccaaaaca tctgccagcc gcttgcgccg agtatcaatg
    18121 ccgcctgttc ttcgctgtcg ccctgagcct gcatcagcgg gatgatttcg cgtgcgacaa
    18181 aggggaaagt aacgaacagc gtcgccagaa taatgccggg gatggcgaag ataatctgta
    18241 tgccttgcgc ttcgagccag ccacccaatg ccgtgtgcgc gccgaacaat aagacgaaca
    18301 ttaaaccggc caccacgggc gatacggaaa acggcaaatc gagcagggtg gtcagcaact
    18361 gcttgccgcg aaaatcaaaa cgggtcagca gccacgccat cgccacaccc aatacagcat
    18421 tgacgggaac gacaatcagc gcggtaatca gcgtcaattt gatggcagac cacgcttcgg
    18481 gatcgtttaa ggatttcagg tacaaatccc aaccgccttt taaggcttcg taaaacacgg
    18541 cgacgagcgg cacgaccagc atcagcagca gaaagcccag cgcggcggca atcagcaaca
    18601 cgcgcagccg gcgcggttcg gtcaggttgg gattggcgga atagggtttc atggcggtgg
    18661 tgttccccgg ttggttgttt taaggtgttc ggatgccgtc tgaaaaaccg ttttttgtgg
    18721 ggcggattcg ttttcagaca accttttatt gattaaggga ataaaaacgt ctagctaccg
    18781 tcattcccgc gcaggcggga atccaccgca gggcaacagg aaaacagaaa ataaataagg
    18841 cagccgaaat tcaccaatgg attcccgcct gcgcgggaat gacggtaaca ggtatttcag
    18901 acgacctcaa cccttcgcgc ccgaacgcct gcccaacgcc cactgcatca cgttcagcgc
    18961 aaacagaatc acaaacgaaa ccagcagcat aaacaacgcc accgccgacg cgccctgcac
    19021 gtcgaactgt tccagcttgc ccgtaataat cagcggtagg atttcggaaa ccatcggaat
    19081 gttgcccgcg ataaaaatca ccgagccgta ttcccccgtt gcccgcgcga acatcattcc
    19141 cgcgccggtc aagagtgccg gcgtgatttc cggcagcagc acgcggcgga acgtcgtaaa
    19201 gcggtttgcg cccaaagtcg ccgccgcttc ctcatattcg cccgataatt cctccaacac
    19261 cggctgcacg gcgcggacga taaagggcag gctgacgacg accagcgcaa tccaaatgcc
    19321 gacgggcgta aacgcgattt tgatgcccaa aggctcgaaa aaacggccta tccaaccgtt
    19381 gggcgcatac agggttgcca acgcgatacc cgtaaccgcc gtcggcagcg caaacggcaa
    19441 atcgaccagc gcgttcgcca gacccttgcc cgggaattca taacgcacca atacccacgc
    19501 caccagcgtg ccgaacacga cattggtcag catcgcataa aacgacatcc gcaaactcag
    19561 ccacaccgcc gccaacacgt tcggctcggc aatcgtgttc caaaagccgc cccagccgat
    19621 ttccgccgcc ttcgccgcca tcatcgcaaa cggcaagacc acaagcagcg acaggcacaa
    19681 tacggtcaga ccaaggctga gtttgaagcc gggcagtacg ccgggcgttt tgagcggtaa
    19741 cataaaacaa tgctgaaaat aaggaaaagg aaggactact ttaacgatgc cgtccgaaaa
    19801 acggaaagaa tggaaagttt ggtgcaaaga cgaatttgtt ataaagcggt tggcagtttc
    19861 tcaagcgggc gcgatgtttt aaaatatagt ggattaaatt taaatcagga
//
LOCUS       DAWFJW010000027        19874 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFJW010000027 DAWFJW010000000
VERSION     DAWFJW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.027"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(108..1277)
                     /locus_tag="WGR19_000799"
     CDS             complement(108..1277)
                     /locus_tag="WGR19_000799"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0798353.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1460..1570)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1623..2519
                     /locus_tag="WGR19_000800"
     CDS             1623..2519
                     /locus_tag="WGR19_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0798354.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2563..3627)
                     /gene="tsaD"
                     /locus_tag="WGR19_000801"
     CDS             complement(2563..3627)
                     /gene="tsaD"
                     /locus_tag="WGR19_000801"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0798355.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3718..4905)
                     /gene="ccsB"
                     /locus_tag="WGR19_000802"
     CDS             complement(3718..4905)
                     /gene="ccsB"
                     /locus_tag="WGR19_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0798356.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGDIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4898..6850)
                     /locus_tag="WGR19_000803"
     CDS             complement(4898..6850)
                     /locus_tag="WGR19_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0798357.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7127..7750)
                     /locus_tag="WGR19_000804"
     CDS             complement(7127..7750)
                     /locus_tag="WGR19_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0798358.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7956..8585
                     /locus_tag="WGR19_000805"
     CDS             7956..8585
                     /locus_tag="WGR19_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0798359.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8706..11102)
                     /locus_tag="WGR19_000806"
     CDS             complement(8706..11102)
                     /locus_tag="WGR19_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0798360.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11254..12369
                     /locus_tag="WGR19_000807"
     CDS             11254..12369
                     /locus_tag="WGR19_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0798361.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12372..12971
                     /locus_tag="WGR19_000808"
     CDS             12372..12971
                     /locus_tag="WGR19_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0798362.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12972..13619
                     /locus_tag="WGR19_000809"
     CDS             12972..13619
                     /locus_tag="WGR19_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0798363.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13637..14182
                     /locus_tag="WGR19_000810"
     CDS             13637..14182
                     /locus_tag="WGR19_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0798364.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENALDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14201..16558
                     /locus_tag="WGR19_000811"
     CDS             14201..16558
                     /locus_tag="WGR19_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0798365.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQQ
                     AAAPAKQQAAAPAKQQAAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHI
                     IVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPG
                     YFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASD
                     SVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKD
                     IADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIV
                     TDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTS
                     AFGWGVNSGFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTK
                     TLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDG
                     QIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLT
                     KVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY"
     gene            17774..18286
                     /locus_tag="WGR19_000812"
     CDS             17774..18286
                     /locus_tag="WGR19_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0798366.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRQT
                     VQTLLKRLSR"
     gene            18366..19445
                     /gene="aroB"
                     /locus_tag="WGR19_000813"
     CDS             18366..19445
                     /gene="aroB"
                     /locus_tag="WGR19_000813"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0798367.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT         5310 a         5685 c         4544 g         4335 t
ORIGIN      
        1 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatataaaa
       61 atgccgtctg aactgttcag acggcatttt tgattttcaa accggaatta cagccccgct
      121 gccgccctca atgcagcagc tttgtcggtg cgctcccaag tgaactcagg ttcttcgcgg
      181 ccgaaatgtc cgtaagcggc ggatttactg taaatcgggc gcaagaggtc gagcatttgg
      241 acgatgcctt tggggcgcag gtcgaaatgt tcgcgaacta aggtaatcag tttttcttcg
      301 ctgattttgc ctgtgccgaa agtatcgatg gaaatcgaag tcggttcggc aacgccgatg
      361 gcgtaggaaa cttggatttg gcattgggtt gccaatcctg cggcgacgat gtttttcgcc
      421 acatagcggc aggcgtaggc ggcggaacga tccactttgg acgggtcttt gccggagaat
      481 gcgccgccgc cgtgcggagc cgcgccgccg taggtatcaa cgatgatttt acggccggtc
      541 agaccgcagt cgccttgcgg gccgccgatg acgaagcggc cggtcgggtt gatcaggtat
      601 ttggtttcgt cggtcagcag ttcagacggc aggacgggtt tgataatctg ctcaatgacg
      661 gcgtttttca gctcttcata accgatttcc gggtcgtgtt gcgtagagag gacgacggta
      721 tcaatgcgtt ttaccttacc ggtttcgctg tcgtaaacca cggtcagttg ggctttggca
      781 tcagggcgca gccaaggcag gcggccgtct ttgcgtaatt cgctttgacg ctgcatcagg
      841 cggtggctgt aatagatggc aaacggcatc agggtcgggg tttcgtcgca ggcatagccg
      901 aacatcaaac cttggtcgcc agcgccttgg ttcaagtcaa tgccttcgcc ttcgttcacg
      961 ccttgggcga tgtccggaga ttgctggtcg tagtacacgc cgaccgcgca gccgttggca
     1021 tcaaagccca gctcggagga gttgtagccg atgcgtttga tggtttcgcg tgcgactttg
     1081 atgtagtcca cttgggcggt ggtggtgatt tcgcctgcca atacgcacaa gcctgtgttg
     1141 accaaagttt ccgccgcgac acgcgctttg gggtcttgcg ccagaatcgc atccaaaata
     1201 gcatcggata cttggtcggc cactttgtcc ggatggcctt cggataccga ttcggaagta
     1261 aacagatatt cgctcattga gattttcctt gaaaaacaaa ccatcttctt cagacggcat
     1321 gttgtatgaa cataatgtcg acagcgggaa atatagcaaa atttccctat tcataccatt
     1381 cagttgagaa atattcccat ttgaatggca ctttggaatc tctgcccgta cgtttcttac
     1441 aggcaaaaaa attcccgcat caagcgggtt tggattgctc tggtgagcca catcggcttt
     1501 tcaaccgtcc accttacttt tccttttgaa aagcaggttg gcatggaatt cccaactctt
     1561 aatgcagcac gcatcgtagc agaaaaggca tattgccgca atacttccct ttttcagacg
     1621 gcatgtttcg tttacaattc aggctgtttc cccctttgcg aaccgccatg cacatcctgt
     1681 tgaccgccct gctcaaatgc ctctccctgc tgccgctttc ctgtctgcac acgctgggaa
     1741 accggctcgg acatctggcg ttttaccttt taaaggaaga ccgcgcgcgc atcgtcgcca
     1801 atatgcggca ggcgggtttg aaccccgacc ccaaaacggt caaagccgtt tttgcggaaa
     1861 cggcaaaagg cggtttggaa cttgcccccg cgtttttcag aaaaccggaa gacatagaaa
     1921 caatgttcaa agcggtacac ggctgggaac atgtgcagca ggctttggac aaacacgaag
     1981 ggctgctatt catcacgccg cacatcggca gctacgattt gggcggacgc tacatcagcc
     2041 agcagcttcc gttcccgctg accgccatgt acaaaccgcc gaaaatcaaa gcgatagaca
     2101 aaatcatgca ggcgggcagg gttcgcggca aaggaaaaac cgcgcctacc agcatacaag
     2161 gggtcaaaca aatcatcaaa gccctgcgtt cgggcgaagc aaccatcgtc ctgcccgacc
     2221 acgtcccctc ccctcaagaa ggcggggaag gcgtatgggt ggatttcttc ggcaaacctg
     2281 cctataccat gacgctggcg gcaaaattgg cacacgtcaa aggcgtgaaa accctgtttt
     2341 tctgctgcga acgcctgcct ggcggacaag gtttcgattt gcacatccgc cccgtccaag
     2401 gggaattgaa cggcgacaaa gcccatgatg ccgccgtgtt caaccgcaat gccgaatatt
     2461 ggatacgccg ttttccgacg cagtatctgt ttatgtacaa ccgctacaaa atgccgtaac
     2521 gaaaataaaa atgccgtctg aacaatttca gacggcattt tgtcatctga cgatttccga
     2581 cagcggccag cgcggacgga cattgaacgc accgacctcc ctgcccttgc ccaggtgcat
     2641 cgcaccggca aaggcaatca tggcaccgtt gtccgtgcag tatgccatcg gcgggaaaaa
     2701 cacgctgact ttttcggacg gatgtttcgg cttgcctttt ggggtcggga tttgcaccgt
     2761 catattgccg aaagtttcac ggagtttgcg gtttgcaccg accccgccgg cgaccactac
     2821 ggttctgaac cctgtctgca acagggcttt tttcactttc gccgccaaca catcgaccac
     2881 cgcatcttga aacgcacggc aaatatcgtt gcgggtttgc tcgggaatgg tttccgaacc
     2941 gtttgcctca cgcacttttt caacggcagt caaaacagcg gttttcaaac ctgaaaaact
     3001 catctgcaaa tcgtcggaat gaatcatcgg gcgcggaaaa acaaacgctt cgggcctgcc
     3061 cgattccgca agttccgaca gtttcgcacc gcccggatac ggcaagccca gcagtttcgc
     3121 cgttttgtcg aatgcctcgc ccgccgcatc atcgacgctc tcgcccaaaa gcgcgtagtc
     3181 gcctatgccc ctgaccgcca taatctgcgt atgcccgccc gaaaccaaca gcgcgacaaa
     3241 aggaaagtcg ggtttttcct ccgccaacag cggcgacagc agatgtcctt ccaaatgatg
     3301 gacggggatg acgggcttgc ccaatgccaa agccagtgca ttggcgtagc tcgaacccgc
     3361 cagcagcgca ccgcccagac ctggaccttg ggtaaaggca accgcgtcaa tgtcgccata
     3421 cgatgcaccc gcctgcgcca aacagccttc cgtcaacgga acaaggcggc ggatatggtc
     3481 gcggcttgcc agctccggca caaccccgcc gtattcggcg tgcattgcca tctgcgtgtg
     3541 caggcagtgc gcccgcaatc cgcgttccgt atcgtaaagc gcaacgcctg tttcgtcgca
     3601 agaagactcg attcctaata ccaacataat ccgataccgt ccgaaaattg aaaaaacggt
     3661 gcgccaaaaa gcgggaaatg ccgtctgaag cccttcagac ggcatcgtcg caccgtatca
     3721 aagcgttccg taagaatgca gcccgctcaa aaacatattc acgccgataa aggcgaatgc
     3781 ggttacgaac aaaccgataa tcgcccacca cgccagcact ttgccgcgcc aaccggcaac
     3841 cagccgcaag tgcagccaaa cggcgtaatt gagccagacg atgaacgccc acgtctcttt
     3901 cgggtcccaa ctccaatagc gtccccaagc atccgccgcc cacagcgcac ccaaaatggt
     3961 ggcaatggta aagaacagaa agccgacggc aatcgcctta tacataactt cctcaatcaa
     4021 cacggattgc ggcagccacg atttcccgcc ttttccttcc gcacgcaggg aaaccagttc
     4081 ggcaataccg agcatcgcgg aaatgcaaaa cgcgccgtaa ccgataaagt ttgccggaac
     4141 gtggattttc atccaccaag attgcaatgc cggaatcagc ggctggatgg tatgcgcctc
     4201 gcgggacacg ctgtaccaca agacaaatcc aaccacgacc gccataaagc cgaacacgaa
     4261 gccgcccaat ttctgtatgg cgaacttacc ttcataataa agatacatca gcgcggtaat
     4321 caccaaaaac aggatgaaca cttcatacag gttggaaacc ggaatatgcc ccgcatcggg
     4381 acggagcaga tagctttcgt gccaacgtac cagcagaccg gtaaagcctg ctacggcaga
     4441 cacccatgca aacacggttc ccatacccaa cagcgtgttg gtcggcacat tttttacgct
     4501 tgccaaaacc gcgcccgaaa tataggcgaa cagggcgaaa aagacaaagg cgcactgcca
     4561 catgatcgcc gactggctgc tgaggaaata ccgcaacagg aaaatctctg ccgatttgat
     4621 gtcgcctccg tacaaaccga cggcggcata ggcaagcaat acgcttaaag gaacaaacca
     4681 gcgcatcggt ttgaaaaacc aacccaaaaa cacggcaata ccggcacttg cccacaacat
     4741 gaccgtttcg taaatgtcca tatgcatacc ggaacgggtc tgtacgaaaa ccgtagccgc
     4801 aaaaaccagc acggcaaata cccaatccca aagattcaga ttgctgatca aagacttctg
     4861 aatcagcagc tcgtgttccg gaagggtttt atagtgttca gtcatgattc aagtccttgc
     4921 cgagccgttg cagactctcg acgtgttttg gaaattcctt ctgcaaatcc cgttcgctgc
     4981 gggccgaaga catggcaaaa cggattttgc cgtctgaaaa caatacccac gcccgttttt
     5041 cgcgcacata aaacatcaat accgtaccca ataccaacag caccgagccg agatagacca
     5101 aaagcgcacc cggggaacgg gtcatctgca aacccgacga acgcacctcg gaaaacccat
     5161 caagttgcag cagcataggc gcgggatatt cggtcaaacc cgtgtacgca tccatactgt
     5221 gcagcaggaa acgattccgc gcttcatcct gctgccattc gggcaagccg taccggcgta
     5281 tggtttcatc caaagcagcg ttcatcacgc cgtaaagcat ttcgtagaaa tagccctgca
     5341 tcttatcctg ctgctctttc gggatattgg acgtaataaa ttcgtccaat cccaaatagc
     5401 ctttttgtgc aaagatgttc agcgtgtttt ccgcagccag catgaattgt tcgcggattt
     5461 cggcaggtgc gcctttggtt gcgtcggcaa ccagacgttt gcgcccttcc ccatctttca
     5521 aaaactcacg caatgccata aaggtgtccg ctttcaactg cttgtccaag gggatacgca
     5581 gccagcggta ttgctgctgc aagccgctgc gcgtgccggt aatccaaaaa taatcctgtt
     5641 cctgcaaaac cggcagcata tagtttttat attcgaccgc ctgccctgcc gcatcacgga
     5701 tacggtaaac aatggaaggg ccgatattgg tgtatttttt accttcctga gtaacggcgc
     5761 ggacatcgtt cagcgtggat ttcaggcttt tttcccgttc cgcgccctcg ctcatgtcct
     5821 ccacattcat agaagtgaac tgatcgaact caagacgata tttgtgtttg ccaatttcca
     5881 acggaaactg gtgtatggat gttgccttca acacgacagg ctcgcgcgaa gcatcaccca
     5941 aattccacgc cttgaatgtc aaatccgaac cgccgtcggc aaaactcgcc tgataaatcg
     6001 tgatgccgtg caaggtcaaa ggatggttca cgcggatggt gcgctcgagt ttctcaccgg
     6061 ttgccttgtc cgtcacttca atatcgctgg cgaaatcacg cggcataccc gtattgtaaa
     6121 aatcgatatg gaattttttc agtttgactt caaaaggcaa gtcctgaacc aatatcccgt
     6181 tgtcggcatt caggaaaacc acatccgcac tctgcccctc ggaaatattg acgttgcccc
     6241 taaatgagag attggacgca cccaaaatac tttcgggctt gaaatccttg gcataaaccg
     6301 cctgattgtc cggaacaatc cgaccggtca gcatacccag tttcaacagc aggttactgt
     6361 ctatcaaccc gcccaggcaa atgacaatca aagcaacatg ggcaaagata tagccccatt
     6421 tgttcattgt gccttttttg gcggcaatca gaaccgaccc gtcttcacgg ttaatggttt
     6481 ttccctgaaa accttgtact tccagataac gtttggcaac ctcgggcgca atttttacat
     6541 ccaacagcga agaatggcgc atcgccgcca gagatttttc tttaaccttt tcccgaaaag
     6601 acttcatttc gcgccagaac ggcggcacat tgcgaatcag gcacaaactg gtagaaacca
     6661 ccaaaaacat catgataacg acaaaccatg ccgaagcata gacgtcatac agtcccagaa
     6721 aaccaaaaat ctgcgcccaa aaaggtccga atttgaccaa ataatccgtc tgcggctggt
     6781 tttgctgcaa caccgtaccg ataaccgatg caatacccag cagactgagc aaagcgaccg
     6841 caaagcgcat ggagctgaaa aaagcgaacc acggacggga aagaagtggg ggagatctac
     6901 gggatttact cattgtgtgt ttattccgcc atcaggaata tgggaaagca gaattgggca
     6961 aacagaaaac aacgtcccga ttctactgtc ttgatgcttt ttatttcaag acaatgaaga
     7021 cagcctgcat cgattccaac ggttgcgatt gaaaaaactt atcgcagaat tgcctgaagc
     7081 cgtctgaaaa cttttcagac ggcctctaaa acagactatt gcggaattaa cgcaaacctt
     7141 ggataaagtt ggcgaccgct ttcaaatctt cttcagacat acggtttgca atatcttcca
     7201 tgatggtatt tttacgctga ccggacttgt aggcattcat ctgttcaaca atatatgcct
     7261 gatgctgacc gcccaaacgc ggataagcct gaatttcgct tccgcctccc ggcatacccg
     7321 caccgctcgg accgtggcag gacatacacg ccggcacttt tttatcgctc aaaccgccgc
     7381 gatagatttt cgcacccaat tcgggatttt ccttaggatt ggcttcaccg gatttgggct
     7441 gctgtttggc atagaatgcg gatacgttca aaatatcctg atcgctcaaa ttcattacca
     7501 ccggtttcat cacagctgcc gaaccgtggg tgcgtttacc gtcgcggatg ccgatagttt
     7561 gatgatagat gtaagcagta tgctgtgccg ccaaacgcgg atacatcgca atgccgctgt
     7621 taccgtctgc tgcatggcaa gccgcacaaa ccgttgcggc aacctgtttg cctttttcca
     7681 cgtctgcttt gggagatgcg gaaaccgcac cggcagccaa aacaaaggcc aataaagtca
     7741 atcgtttcat ggagtgctcc tgattacagc attggataac gcaacaatgc tctttttata
     7801 ttcaaatacg ggatttttga cccgattaaa accgatgatt ctgcaaacgt gttattctat
     7861 actaaattta cattaaatta ccactgtgtt tcacataaaa ccaaccgcat atttttgctg
     7921 tcggacaaac ggcggcggaa aacaaggata tgcccatgaa cctttttcaa aacgccaaat
     7981 tcttcacgac ggtcaaccac cttaaagacc tgccggacac accgctcgaa attgcctttg
     8041 tcggcaggag caatgccgga aaatccagtg ccatcaatac cctgaccaac catgtccgtc
     8101 ttgcctacgt ttcaaaaaca cccggacgga cgcagcatat caacttcttc gagctgcaga
     8161 acggcaattt tatggtcgat ttgcccggct acggttatgc ccaagtcccc gaagcagtac
     8221 gcgcacattg ggtcaatctg ctcggcgact atctgcaaca gcgcaaacag cttatcgggc
     8281 tggttttgat tatggatgcc cgccatcctt taaaagaact cgacatccgt atgctggact
     8341 ttttccacac gaccggcaga ccggttcaca tcctgctgtc aaaagccgac aaattatcca
     8401 aaaacgaaca gataaaaacc ctgtcccaag tcaaaaaact gctcaaacct tattccgaca
     8461 ggcaaaacat cagcgtacag ctgttttcca gcctgaaaaa acaaggtatt gacgaggcca
     8521 accgaactgt cggaagctgg tttgacgcag cagatgccgc cgcttcctct ccaaaggaaa
     8581 actgacccca attatacaga aaccgtattt cccccacttg accgaccgca aacatttaaa
     8641 aaattgccac tgccaaatct aaaatgccgt ctgaaagact tttcagacgg cattttgcgg
     8701 agtctttaaa acagagaatc caactgctgc tgtttggaac cagtattact cggaagcacc
     8761 ggcgtttcct gcatatcttg gcggacttcg tcatccgccg cctgccgtcc gccttctgcc
     8821 gcgcccccgt catcttcttt tgcccgtcgg gaaggttgcg gcgcaatacc gctgttgtcc
     8881 agcgtcaagc ccggatcggt taccatacgt tccttcatat agtattcgcc attgctgctg
     8941 accacacctt caggcatttt catccccttg ccctgctttc ctttcaacgc aaaacgcata
     9001 tagtccaccc aaaccggcac cgcaatcgta ccgccgtagc cgacacgccc catactctta
     9061 ggtttgtcga agccgatata tacggcagta accacatcag ggttaaaacc gacaaaccac
     9121 gcatccttat tgtcattggt cgtacccgtt ttaccggcaa tatccgttct tcccaacgca
     9181 gctgcccccc ttgccgtacc aacacggacc acatcctgca taatcttata cataatatag
     9241 gcattgcgcg gatcgattgc ctgaggcgca ttttgcccgg ccaccaaagg ttgcatttgg
     9301 gcgcgcaacc tgccgtctct gtcataaatc ttatcgatta cgtgcgaaga aaccctatat
     9361 ccgccgttcg caaatacgct atatgcctcc gccactttca acggcgttgt ctcgcccgta
     9421 cctaaagcca tagacaggct tgccggcagc tcggacgacc tgaagccgaa acgccggata
     9481 tactgttgcg cgtaaccgac accgatagac atcaaaatac ggatggaaac catattcttg
     9541 gaagccgtca gagcctgtct caaagtaatg tagccggaat atctgccgtc tgaattttta
     9601 ggtgtccaaa ccgaaccgtt cggccctttc cccggcaggg aaatcggcgc atcgttaacc
     9661 actgtggacg cggtcatccc cttagataat gccgccgaat agacaaacgg cttaaaggtc
     9721 gaacccggct gccgcattgc ctgaacggca cgattgaatg ttttgctgtg aaaatcataa
     9781 ccgccgacca gcgcgcgcac agctccggtt tttgcatcca gcgaaaccaa agccccctgc
     9841 agcaacggct cttgaaccac cgcccaacgc ccgccgttgt ttttgacacg gatgaccgcg
     9901 cccctgcgga tacggtcctc ccccattttt tcattattga ccgcgcgggc cgcaaaaccc
     9961 aaggcgcgcc tgtcaagcgt aacccgcctg ccgccgggca gctgtatgac gacatttttc
    10021 tttttagtca catccaacac aacggcggga accattttat cgacggtata gagtcccgac
    10081 agatactggc tgacagtctc ctcgacatct tcactcttac tcaaatcgat atagttttcc
    10141 gcaccgcggt agctgctgcc gcgatcgaaa ttccgtagag ccttgcgcaa tgcctcggtt
    10201 gccaccttct gatgatcggc gcggaccgtg gtataaacct taaaaccctg cgtataggca
    10261 tcttcaccgt atttctcata cagttcctga cgcaccattt ccgccacata taaagcactc
    10321 tgatcgattt tccgaacaaa ccgctcgtaa tgcagttcct cattcaacgc ctgatcgcgc
    10381 tgttgcacgg taatcatctt ctcctcgagc atattgttca aaatatactt ctggcgcaac
    10441 ttggcacgtt ccggattaac aatcggatta taggcagacg gagccttggg cagtcccgca
    10501 agcatggcgg cttccgccaa agtcaaatct cggacattct tattgaaata gatttgcgcg
    10561 gcagatgcaa aaccataggc gcgctgaccg aggtaaatct gattgaaata caactcgagg
    10621 attttgtctt tgcttaaaga ctgctcgatt ttataggcaa gcaacacctc attgaatttg
    10681 cgtgtgaacg ttttttcact gctcaaataa aaatttttcg ccacctgctg cgtaatcgta
    10741 cttgcacccg actgcacgct gccggacacg acattgccga cggcagcgcg ggcaacaccc
    10801 caaacatcca ccccccaatg ccggtaaaag cgtttgtcct cggcggcgat aaccgcattc
    10861 cgcaacacct ctgggaaatc gccgattttt gtaaattcgc gccgctgctc cccatacata
    10921 ccgatgactt ccccatccgc cgaataaata gtcaacggca ttttaggctg gtaatgctgc
    10981 aaagaatcca aagacggcag tttcggatac gttaccaaaa tagcaatggc aaccaaaccc
    11041 actccaaata cacaaaaccc aaaaaccaaa ccaaaacaag tcgttaaaat ctttttaatc
    11101 atagctgaat aataatttac cattattggt attaaataaa gtaaaatagc aaccgatttc
    11161 tacaaagcac ggtttcaatg tgcaaagaac aaggaatcca ttacggatac cgaaacggtt
    11221 actcactgta caaataaagc aggaactttc atcatgcgct tgtttaaaag cttgaaaaac
    11281 cctaaaaaaa cagatgccaa gctccctaaa aaatcttcgg gactcaataa ccgcgcggca
    11341 atcggcatcg atatcgacca gcattccatc aaaatggtcc aattgtcagg acgtagttta
    11401 aaccaaattc aattggaaaa atacgtcatt gccaaattac caaagaatat cattcaaggc
    11461 aataaagtcc aaaattacga tcaacttgtt acatatttgc aacaagccta tgccaaactg
    11521 ggtacttcgt gcaaaaacat catcgcgtcc gtcccgcaaa atctggcaac catcgaacaa
    11581 ttgacctaca cagacaaaga tgcagaatta gacctgcagg ggttcgtgga gtcctccatc
    11641 tccgaagtca gctcgatatc gctcgaagaa gccaattacg actatcaggt cttgtcccaa
    11701 tcggccgccg gcgaagctgt gttggccgtc gcatcgagaa aggatgaaat cgaacccctg
    11761 attgacgcat tcaacgccgc cggtatgaaa ttatccgcgc ttgatgtgga cattttcgga
    11821 caatacaacg cctacgcgct atggataaac catttcgccc ccgagcttgc agccgaaaaa
    11881 gtcgccattt tcggcgtata tgccgcacag acctacgcct tggtcatcca agacggaaaa
    11941 atcctataca aacaggaaac ctccgtcagc gaagaacagc tcaaccaact catccagcgc
    12001 acctatcagg taacagaaga aaaagcggaa gaaatcatca actccccgca aaaaccttcc
    12061 gattaccaag aaagcgtggc aaactatttc aaccagcaga ttacccaaga aatacaaagg
    12121 gtcttgcagt tttattacac cacgcagacc gcagacgata tgaccgacat caagcatatc
    12181 ctgctgaccg gggaagcggc gcgccaggaa ggcatcgccc aaaccgtcgc ctcacaaacc
    12241 aatgcagatg tacaatgcgt ccatcccgcg cgttattttg cggacaacct caaaacagac
    12301 aaacaacaat tcgaacttga tgcgccgaca ctgaccaggg cgttcggttt ggcggtacgg
    12361 ggattataat tatgaacaat ttaatcaaaa tcaacctcct cccctacagg gaagagatga
    12421 acaagcgcaa acagcagcag tttaaaacgc tgatgtacgg tgccgtgctg acgggcgttg
    12481 ccgccgttgc ggcaacctac ctgtttatcg acaatatgat caataaccag tcggaaagaa
    12541 acacgctgct ggaaacctcc atcgcacact tggataccga gctgtcggaa atacaaaagc
    12601 tcaaacagga aaaagatgcc ttcctgatta agaaaaacaa aatcgaggag ctccagctca
    12661 aacgcctcca agccgcaaaa atcctcgaca gcctgaatga ggccgtcccc ggaagcacct
    12721 acctgacctc gctggatgcc gttaccgccg actcttatcg gctcagcggc aggacatcca
    12781 gcgacaaccg cgttgccgcc atgatgaggg cgatgcccaa taccggcata ttcaagcaac
    12841 ccgaattgtt aagcatcaag aaaaacaatt cgcatcaaga atttaccctt caggcaacat
    12901 tacaacccat cgtaaaggcg gccgaatcca aagagaatcc ggcttcggga aacgcacagg
    12961 aggcaaactg aatggcttct aaatcatcta aaaccaactt ggatctcaac aaccttcacc
    13021 tgctcaacct tcctgccagg ctttttatcg ccctgctggc cgttgccgcc gtgctggggc
    13081 tcggttatgc cggattgttc aaaagccaga tggaatccct tgaggaatac gaagcaaaag
    13141 aaaccgaact gaaaaacacc tacaaacaga aaagtatcga cgcggccagc ctgaacaacc
    13201 tgagggacga acttgcctca atccgctctg ccttcgatat catgttgaaa cagctgccga
    13261 cagatgcaga aattcccaac ctggttcaag agcttcatca ggcaggttcg agcaacggcc
    13321 tgcgcttgga cagcgttatg ccccaacctc ccgtagatga cggccccatc aaaagattac
    13381 cctattccat ttccattacc ggaaattacg aacagatcag ccaatttacc cgcgatgtcg
    13441 gcagcctctc ccgaatcatt acccttgagt cgctgaaaat cgcccaatct ccggaaaacg
    13501 gcggcaatcc tgacggcaag agcagcatcc tgaacctcag tgccattgcc accacctacc
    13561 aagcaaaatc cgtagaagag cttgccgcag aagcggcaca aaatgccgag caaaaataac
    13621 ttacgttagg gaaaccatga aacactatgc cttactcatc agctttctgg ctctctccgc
    13681 gtgttcccaa ggttctgagg acctaaacga atggatggca caaacgcgac gcgaagccaa
    13741 agcagaaatc atacctttcc aagcacctac cctgccggtt gcgccggtat acagcccgcc
    13801 acagcttaca gggccgaacg cattcgactt ccgccgcatg gaaaccgaca aaaaagggga
    13861 aaatgccctc gacaccaagc gtattaaaga aacgctggaa aaattcagtt tggaaaatat
    13921 gcgttatgtc ggcattttga agtccggaca gaaagtctcc ggcttcatcg aggctgaagg
    13981 ttatgtctac actgtcggtg tcggcaacta tttgggacaa aactacggta gaatcgaaag
    14041 cattaccgac gacagcatcg tcctgaacga gctgatagaa gacagcacgg gcaactgggt
    14101 ttcccgtaaa gcagaactgc tgttgaattc ttccgacaaa aacaccgaac aagcggcagc
    14161 acctgccgca gaacaaaatt aagaagagga ttactccatt atgaatacca aactgacaaa
    14221 aatcatttcc ggtctctttg tcgcaaccgc cgcctttcag acagcatcgg caggaaacat
    14281 tacagacatc aaagtttcct ccctgcccaa caaacagaaa atcgtcaaag tcagctttga
    14341 caaagagatt gtcaacccga ccggcttcgt aacctcctca ccggcccgca tcgccttgga
    14401 ctttgaacaa accggcattt ccatggatca acaggtactc gaatatgccg atcccctgtt
    14461 gagcaaaatc agtgccgcac aaaacagcag ccgtgcgcgt ctggttctga atctgaacaa
    14521 accgggccaa tacaataccg aagtacgcgg gaacaaagtt tggatattca ttaacgaatc
    14581 ggacgatacc gtgtccgccc ccgcacgccc cgccgtaaaa gccgcgcctg ccgcaccggc
    14641 aaaacaacag gctgccgcac cgtctaccaa gtccgcagta tccgtatccg aaccctttac
    14701 cccggcaaaa caacaggctg ccgcaccgtt taccgagtcc gtagtatccg tatccgcacc
    14761 gttcagcccg gcaaaacaac aggcggcggc atcagcaaaa caacagacgg cagcaccagc
    14821 aaaacaacag gcggcagcac cagcaaaaca acaggcggca gcaccagcaa aacaacaggc
    14881 ggcagcacca gcaaaacaac aggcggcagc accagcaaaa caaaccaata tcgatttccg
    14941 caaagacggc aaaaatgccg gcattatcga attggcggca ttgggctttg ccgggcagcc
    15001 cgacatcagc caacagcacg accacatcat cgttacgctg aaaaaccata ccctgccgac
    15061 cacgctccaa cgcagtttgg atgtggcaga ctttaaaaca ccggttcaaa aggttacgct
    15121 gaaacgcctc aataacgaca cccagctgat tatcacaaca gccggcaact gggaactcgt
    15181 caacaaatcc gccgcgcccg gatactttac cttccaagtc ctgccgaaaa aacaaaacct
    15241 cgagtcaggc ggcgtgaaca atgcgcccaa aactttcaca ggccggaaaa tctcccttga
    15301 cttccaagat gtcgaaatcc gcaccatcct gcagattttg gcaaaagaat ccgggatgaa
    15361 cattgttgcc agcgactccg tcaacggcaa aatgaccctc tccctcaaag acgtaccttg
    15421 ggatcaggct ttggatttgg ttatgcaggc acgcaacctc gatatgcgcc aacaagggaa
    15481 catcgtcaac atcgcgcccc gcgacgagct gcttgccaaa gacaaagcct tcttacaggc
    15541 ggaaaaagac attgccgatc tgggcgcgct gtattcacaa aacttccaat tgaaatacaa
    15601 aaatgtggaa gaattccgca gcatcctgcg tttggacaat gccgacacaa ccggaaaccg
    15661 caatacgctt gtcagcggca ggggcagcgt gctgatcgat cccgccacca ataccctgat
    15721 tgttaccgat acccgcagcg tcatcgaaaa attccgcaaa ctgattgacg aattggacgt
    15781 acccgcgcaa caagtgatga ttgaggcgcg tatcgtcgaa gcggcagacg gcttctcgcg
    15841 cgatttgggc gttaaattcg gcgcgacagg caagaaaaag ctgaaaaatg atacaagcgc
    15901 attcggctgg ggggtaaact ccggcttcgg cggcgacgat aaatgggggg ccgaaaccaa
    15961 aatcaacctg ccgattaccg ctgccgcaaa cagcatttcg ctggtgcgcg cgatttcctc
    16021 cggtgccttg aatttggaat tgtccgcatc cgaatcgctt tcaaaaacca aaacgcttgc
    16081 caatccgcgc gtgctgaccc aaaaccgcaa agaggccaaa atcgaatccg gttacgaaat
    16141 tcctttcacc gtaacctcaa tcgcgaacgg cggcagcagc acgaacacgg aactcaaaaa
    16201 agccgtcttg gggctgaccg ttacgccgaa catcacgccc gacggccaaa tcattatgac
    16261 cgtcaaaatc aacaaggact cgcctgcgca atgtgcctcc ggtaatcaga cgatcctgtg
    16321 tatttcgacc aaaaacctga atacgcaggc tatggttgaa aacggcggca cattgattgt
    16381 cggcggtatt tatgaagaag acaacggcaa tacgctgacc aaagtccccc tgttgggcga
    16441 catccccgtt atcggcaacc tctttaaaac acgcgggaaa aaaaccgacc gccgcgaact
    16501 gctgattttc attaccccga ggattatggg tacggccggc aacagcctgc gctattgatg
    16561 cgtcaaaata agggcatatg ttttacggca tatgcccttt ctttatgctt tttgccgcga
    16621 ccgaaatgcc gtcattcccg cgcaggcggg aatccaactt gtccggttcc ggttgttttt
    16681 cgtctcgtaa cttttgagcc gtcattcccg cgaaagcgga aatccagtcc gttcggtttc
    16741 agtcatttcc gataaattcc tgttgctttt catttctaga ttcccacttt tgtgggaatg
    16801 acggcggaag gggtaaatcc tcgcaatcca aagcctcgtc atttctacaa aaaacagcaa
    16861 cccgaaacag caacttaaaa ccccgtcatt cccgcgcagg cgggaatcca acttgtccgg
    16921 tttcgcttgt ttttcgtctc gtaacttttg agccgtcatt cccgcgaaag cggaaatccg
    16981 gtctcttcag tttcggtcat ttccgataaa ttcctgttgc ttttcatttc tagattccca
    17041 cttttgtggg aatgacggcg gaagggataa atcctcgcaa tccaaagcct cgtcatttct
    17101 acaaaaaaca gcaacccgaa acagcaactt aaaaccccgt cattcccgcg caggcgggaa
    17161 tctaggtctg tcggttcagg aacttatcgg ataaaacggt ttctccaacc ctgcgttcta
    17221 gattcccact ttcgtgggaa tgacgggata tgggtttccg tgcggacgtg ttcggatttc
    17281 cgcctgcgcg ggaatgacgg cgacagatgc ccaacggtct ttatagtgga ttaacaaaaa
    17341 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcacttggtg
    17401 tttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaacgct gtactggttt
    17461 ttgttaatcc actatagtat tgataaacat attatcttca atatattcaa ttggataatt
    17521 gtttacctaa gcaaagataa ttgccttttc ctgacaaata agtgaaatca acggattgtc
    17581 aaaacacagc ctgaaataaa aaacctccct gatttctttt atttttcctt aaaatcagaa
    17641 aggttcggga tggtcgggtt atttttccaa acgtaccgct gccctgccga tttcgtataa
    17701 aattccgccg taacccgaca agcccgaacc ctgtcgcccc gaaaggcggg gtgtcaaaca
    17761 ttaaggaatt gtgatgaaaa actttaacgg caaactcatc ctcatcggac tgatgggcgc
    17821 gggcaaaacc acgctgggcc ggcaaatggc gcagcggctg gattaccgtt tttacgacag
    17881 cgatcacgaa atcgccgtag ccgccggcgt tcccatcccc accatatttg aaatggaagg
    17941 cgaacaggga ttccgttcgc gcgaaaccgc catactcaaa aagctggtta tcctgcccca
    18001 tatcgtcctg tccaccggcg gcggcgcggt gttaaaagaa gaaaaccgcg cccttatccg
    18061 caaaagcggc acggtcgtct atctgcacgc cccgcccgaa accctgctcg aacgcacgcg
    18121 ctgcgacaac agccgtcctt tgctgcaagt tgccgatcct ttggcgaaat tacgtgaact
    18181 ctacgccgca cgcgaccccg tttaccgcca aaccgccgac tttaccgtag aatcggcaaa
    18241 ctgccggcaa accgtgcaaa ccctgctcaa acgcttatcc cgataaaccg gcatatgcgc
    18301 cgcgcccaga aaaccaagcc gcgccccgcc cggcgggccg gcggttcaaa ctttaaggaa
    18361 caacaatgaa aacactgacc gtacacaccc cgtcccacag ctaccccatc tttatcggca
    18421 acggactgct gccgcaggca ggaagcctgc tcaaaccgca tttgggcaaa cgcgccgcca
    18481 tcatcgccaa cgaaaccgtc gccccgctct acctcggcac gcttcagacg gcattggatg
    18541 cggcaggcgt atcccatttc agcatcatcc tgcccgacgg cgaggcgcac aaaaactggc
    18601 agacgctcaa cctcatcttt gacgggctga tgcaaaaccg cgccgaacgc aaaaccacat
    18661 taatcgcact gggcggcggc gtgatcggcg acatggtcgg ctttgccgct gccacctacc
    18721 agcgcggcgc accgttcgtt caaataccga ccacactgtt gagtcaagtc gactcatcgg
    18781 tgggcggcaa aaccgccatc aaccacccgc tcggcaaaaa tatgatcggc gcattctacc
    18841 agccgcaggc ggtgcttgcc gacttggaca cgctgcacac cctgcccgtc cgcgaattgt
    18901 ccgccggtat ggcggaagtc atcaaatacg gcgcgctcgg cgacatcggt tttttcgaat
    18961 ggctcgaaca aaacatatcc ggactgatga cgctcgatcg ggaaaaactc gcccaagccg
    19021 tgtaccgctg ctgccaaatg aaggcagaca tcgtcgccca agacgaaacc gaacagggca
    19081 tacgcgcatg gctcaacctc ggacacacct tcggacacgc cattgaaacc gagatgggtt
    19141 acggcacttg gctgcatgga gaagccatcg ccgccggctg cgtgttggcg gcgcgtttgt
    19201 ccgaacaact gggcaaaacc tccgccgcag ataccgcgcg gctcgccgcc ctactcgaag
    19261 ccgccggact gccgtccgca ccgcccgtgt ttgcctttga aaaatggctc gaacatatga
    19321 gccacgataa aaaagtcagc ggcggcatca tgcgctttat cggtctgaac cggctgggcg
    19381 aagccaacat caccgaaatt accgacacgg acatcctccg ccgcaccctg caaccgtatc
    19441 tctgatttcc tctgccgatg tgctgccgcg cgggtttgac gcacgatgat gattttccat
    19501 catctttctc cgtaaaagcg ggaatccggt ccgttcggtt tcggtcgttt ccgataaatt
    19561 cctgctgctt ttcatttcta gattcccact ttcgtgggaa tgacggtgga gaggtttttg
    19621 ttgtttcgga gaagtttctg caaccctaga atctcgttat ttccacaaaa aacagaaacc
    19681 cgaaacagca acttaaaacc tcgtcattcc cgcaaaagcg ggaatccggt ccgttcagtt
    19741 tcggtcgttt ccgataaatt cctgctgctt ttcatttcta gattcccact ttcgtgggaa
    19801 tgacggcgga gaggtttttg ttgtttcgga gaagtttctg caaccctaga atctcgttat
    19861 ttccacaaaa aaca
//
LOCUS       DAWFJW010000028        19568 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFJW010000028 DAWFJW010000000
VERSION     DAWFJW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.028"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            153..1058
                     /locus_tag="WGR19_000814"
     CDS             153..1058
                     /locus_tag="WGR19_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0798368.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGV
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEVEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSSH"
     gene            complement(1113..1219)
                     /locus_tag="WGR19_000815"
                     /pseudo
     CDS             complement(1113..1219)
                     /locus_tag="WGR19_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1317..2336)
                     /gene="lepB"
                     /locus_tag="WGR19_000816"
     CDS             complement(1317..2336)
                     /gene="lepB"
                     /locus_tag="WGR19_000816"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0798369.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENRYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIH"
     gene            complement(2480..4273)
                     /gene="lepA"
                     /locus_tag="WGR19_000817"
     CDS             complement(2480..4273)
                     /gene="lepA"
                     /locus_tag="WGR19_000817"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0798370.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4416..5117)
                     /locus_tag="WGR19_000818"
     CDS             complement(4416..5117)
                     /locus_tag="WGR19_000818"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0798371.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPARFASDGILIETAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            5303..6415
                     /locus_tag="WGR19_000819"
     CDS             5303..6415
                     /locus_tag="WGR19_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0798372.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            6449..7426
                     /locus_tag="WGR19_000820"
     CDS             6449..7426
                     /locus_tag="WGR19_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0798373.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDESENGRKLLQIKIDAVRKII
                     DNVYLTSVRGGLRVVLIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRDRGVAEPEEFLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLFQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            7430..7780
                     /locus_tag="WGR19_000821"
     CDS             7430..7780
                     /locus_tag="WGR19_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0798374.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            7785..8564
                     /locus_tag="WGR19_000822"
     CDS             7785..8564
                     /locus_tag="WGR19_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0798375.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRAV"
     gene            8590..8804
                     /locus_tag="WGR19_000823"
                     /pseudo
     CDS             8590..8804
                     /locus_tag="WGR19_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
     gene            8905..9216
                     /gene="grxD"
                     /locus_tag="WGR19_000824"
     CDS             8905..9216
                     /gene="grxD"
                     /locus_tag="WGR19_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0798376.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            9322..9948
                     /gene="upp"
                     /locus_tag="WGR19_000825"
     CDS             9322..9948
                     /gene="upp"
                     /locus_tag="WGR19_000825"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0798377.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            9971..10291
                     /locus_tag="WGR19_000826"
     CDS             9971..10291
                     /locus_tag="WGR19_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0798378.1"
                     /translation="MNFQDYLATFPPIDHLTGLDVQDAGGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGRLDKEAAEQGLIWFVEHVADACAHPGKHPNIDLLEKVVQSGETWLLKP
                     LAAR"
     gene            10478..10900
                     /locus_tag="WGR19_000827"
     CDS             10478..10900
                     /locus_tag="WGR19_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798379.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFAW"
     gene            10918..11679
                     /locus_tag="WGR19_000828"
     CDS             10918..11679
                     /locus_tag="WGR19_000828"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0798380.1"
                     /translation="METAKPVMLIVRPSGRAKDDVETCRRAGWQAEMLSPIEIETDKA
                     SLKRLPEMYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            11694..13028
                     /locus_tag="WGR19_000829"
     CDS             11694..13028
                     /locus_tag="WGR19_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0798381.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETDTILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQSDLNNAETAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            13025..14242
                     /locus_tag="WGR19_000830"
     CDS             13025..14242
                     /locus_tag="WGR19_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0798382.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRLLADADDAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYSRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGETQKAEAQRSLVLA
                     SVAEENRPSAETH"
     gene            14322..15386
                     /locus_tag="WGR19_000831"
     CDS             14322..15386
                     /locus_tag="WGR19_000831"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0798383.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPFALFGTPESIRTEVARILVDYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(15449..17338)
                     /locus_tag="WGR19_000832"
     CDS             complement(15449..17338)
                     /locus_tag="WGR19_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0798384.1"
                     /translation="MLRTYLNQLTPPELADSVENTVNGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGILSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVIKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     TDKPDKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQNEASDWQPEFIHFFEAGE
                     KYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKA
                     NCNFALHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIH
                     HFDEIYEKLTALLENKQISTLVVNSQTESDFDLERGFNIIIGGNVIGRGLTIPKLQTV
                     YYSRTAKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKN
                     SGGNIQVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKD
                     EIQSDLYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGR
                     KLSRATGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLV
                     YQSAK"
     gene            complement(17339..18379)
                     /locus_tag="WGR19_000833"
     CDS             complement(17339..18379)
                     /locus_tag="WGR19_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HEZ0798385.1"
                     /translation="MNTVFSNIANAKITDKSLNKVWMDLFMSADDMLMATGYVSNDAV
                     KELHKILEESDHKQKLSLMVGMHYLEGFSRPQYSSLCELNDFLQDEQRGMVYVSPWVK
                     FHGKMYSFQNQREIHTLIGSANLTCFWDKAERTYETMVHLDDYQIASKLREDIAQTIK
                     QLGIPINKADKPVEFKEHNVHLEQCLGVEKISKERVEKLFTQTSQYYFELPVKTEPRS
                     SLNRFFAEGRKNQRGFYLPRPWYEAELIVSTRFLTAEGYPKEKNFTVITDDGWQFECN
                     TTGQNGKNFSSKGDQTIFGKWVKGRLEATGCLKTGEPVTEETLRQYGNDHFEFRSTDN
                     PDVWLLSFKGKN"
     gene            complement(18376..19500)
                     /locus_tag="WGR19_000834"
     CDS             complement(18376..19500)
                     /locus_tag="WGR19_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0798386.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGYETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQIDPNNPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFHHPEPTHGENTDYGLLPYVTAGQAISDIPENA
                     NNHEQLKISEQTKKRLNLIPEGGNYSDIPRDHPLYVKSMLSLVYRRMHRDKPSTTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDNFEFMGSTTEVRRQIGNAVPPQGVVELA
                     KAILPVFSDGYHKVNLHEKLEAEKNVFFQDRLNKIRGGKR"
BASE COUNT         4911 a         4837 c         4886 g         4934 t
ORIGIN      
        1 gggttttatc tggcaggacg gttttttgcc ccaacggaaa atagcctgcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtgtttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gtgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtgtccggg ctgatgcttg
      661 ccctgtcgat ggtgttcgtc cgcaagactc atgaagtcga gccggtggcg cttttccctt
      721 caatgatgat tttgagttta ggcggcgcgg tatcgttggt tgttccggca ttgctgatgg
      781 atggcggcgc gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 gattgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttatctcgg ttcgctgaaa cagcagtctt cacattgatt tcatcagggc aatattggca
     1081 gttcgccgtg caaaacctgc gttaccggac tgatatagtg aattaaattt aaaccggtac
     1141 agcgttggct cgccttgccg tactatttgt actgtctgcg gctcgccgcc ttgtcctgat
     1201 ttaaatttaa ttcactatag gtaggaaaat ccgacaacgt tagacccgcc tgcaaaggta
     1261 aggaatagca aatgccgtct gaaactattt tcagacggca ttcttggctt cccggcctaa
     1321 tggattgccg taccggccct gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg
     1381 ccgacaacca gcttgtcatc cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg
     1441 ttgtcgccca tagcgaaata gcgtccttcg ggaactttgc acacgaaacc gctgccgtcg
     1501 tcggcatatt ggcagtgttc caaaccgctt tgctctatgg aatatctgtt ttcagacata
     1561 atatcggagg tatatttgcc caatacgggc agggaaacgg caggctgtcc ttcttttttc
     1621 agaatattga aggatttgcc gtccagaccg ctgcggaaca tatccgtgtt gtggatttcg
     1681 gaagggtcgg tgtcgtcggg ataacggtat gtgccgtcag gaatgtcgga agtgggtttg
     1741 ccatttaccg tcaaaatctt atcccgatat tcgaccacat cgcccggaat gccgacaata
     1801 cgcttgatgt aggtcatctc cggctgcaga ggataattaa aaacaacgac atcgccccgt
     1861 tcgattttgc ctgtaggaat aaatatattg tttaaaacgg gtacgcgcag gccgtaggaa
     1921 aatttgccga ccaaaatgaa atcgcccttg atcaggcccg ggcgcatcga gctggacggg
     1981 atttggaacg gttcggcgat aaacgaccgg atgaggaaca ataccaaaac ggtagggaag
     2041 aaactgccga aataatcgcc gaagtggctg ctttccgaga tttcgggatg agtcttcagg
     2101 cggtatttat atacccccca agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg
     2161 gtaaagctca taaacaggga caaagcggca aacacgccga ccgctgtcag gatataggcg
     2221 tattcaaggc cggaactcca ttctccgttt tcctgtcgtt tcttgtcgct tttgaaataa
     2281 aggatgatgc cggcaagcag cgcggcagcc gcacccgaca ttaacattgt gttcattgtt
     2341 gttccttaat gcttaaaacc cgcccggccg tgcaaccgtt ttaaggcggc aaattgcaaa
     2401 atttgtttgc gggcgcgtgc ccctgaaatc agggcggttt gaggggtgtt cccgacgcgc
     2461 cgccctatgt gccgaaatat tatttgtcgc tcacctgcaa aatcgccaag aacgcgcttt
     2521 gcggaatttc cacgttgccc acttgtttca tacggcgttt gcctgccttt tgtttttcaa
     2581 gcagtttttt cttacgcgta atatcgccgc cgtaacattt cgccaagacg tttttacgca
     2641 aggctttgac gttttcgcgg gcgataatct ggctgccgat ggcggcctgt acggcgatgt
     2701 cgaacatctg gcgcggaatc agttcgcgca ttttcgatgc cagctcgcgg cctcggtgaa
     2761 cggcgctttg acggtgcaca atcaggctca aggcatcgac tttttcgccg ttgaccataa
     2821 tatccagctt aatcaaatca gacggttgga actctttgaa atggtagtcc aacgaagcat
     2881 agccgcgcga agtggatttg agtttgtcga aaaagtccat caccacttcg ttcatcggca
     2941 aatcataagt cagcatcact tggcggccca tgtactgcat attgacttgc acgccgcgct
     3001 tttggttgca caaagtcatg acgttgccga catattcctg cggcacaagg atggtcgcgg
     3061 taataatcgg ctcaagaatg gtttcgatgc tgccgatgtc gggcagtttg gacgggtttt
     3121 caacttcgat tttttcgccg cttttcaaca cgacttcata aaccaccgtc ggcgcggtgg
     3181 taatcaaatc catatcgaac tcgcgttcca aacgttcctg cacgatttcc aagtgcagca
     3241 gacccaagaa gccgcaacgg aagccgaagc ccaatgcctg ggaaacttca ggctcaaatt
     3301 tcaacgaagc gtcgttaagc tgcaattttt ccaaagcgtc gcgcaaggct tcgtagtcgt
     3361 ggctttccac gggataaagg cccgcgaata cttggctttg tacttcttgg aagccgggca
     3421 gcggctcagt ggcagggttg gcaaccagtg taaccgtatc gccgactttc gcctgaccca
     3481 attcttttac gccggtaatc agaaagccca cttcgccggc tttgagttct tgtttttgaa
     3541 ctgatttcgg cgtgaatacg cccagctgct caacctgcgt ttccgccttg gtgctcataa
     3601 agcgtacttt gtctttcagc ttgatggtac cgtttttcac acggatcagc ataaccacgc
     3661 cgacatagtt gtcaaaccac gaatcgacaa taaccgcctg caacggtgca ttttcatcgc
     3721 cggtcggcgc agggattttg gcaacgattt cttccaaaac atcttccaca ccgatgccgc
     3781 ttttggcgga acattgcacc gcgccgacgg catcgatgcc gatgatgtct tcgatttcct
     3841 gttccacgcg ttcggggtcg gcggcaggca agtcgatttt gttcaaaaca ggcacaactt
     3901 ccacgcccaa atcaatcgcg gtatagcagt tcgccacggt ttgcgcttcc acgccttgcg
     3961 acgcgtcaac caccaaaagc gcgccttcgc aggcggacaa ggagcgggaa acttcataag
     4021 agaagtcgac gtgtcccggc gtgtcaatca aattaagctg atacacctgc ccgtcgcgtg
     4081 ctttgtagtt gagcgcggcg gtttgcgctt tgatggtaat gccgcgctct ttttcgatgt
     4141 ccatagaatc gagtacctgc gtactcattt cgcgcaaatc caagccgccg caatattgga
     4201 tgaagcggtc ggcaagcgtc gatttgccgt ggtcaatgtg ggcaataatg gagaaatttc
     4261 ggatattttt cattgagttg tttttaatgc tgaacggtag gttttagaaa tgccgtctga
     4321 acaaacagcg gtacgtccaa atatcgggcg caacgtggaa atagcccgat attctaacgg
     4381 aaaaccgctg ttttggcata agtttgataa aggtcttata aagatttgac gatttctgcc
     4441 accatttttg cggaatttgc cgccgccgtt ttcaagaact cgtcaaagct gatgtctgcc
     4501 ttttcgtctg ccgaatcgga aaccgcgcgg atgatgacga aaggcgtttc caactgatga
     4561 caggtttggg cgattgccgc cgcttccatt tctaccgcct taacttcggg gaagtgcttg
     4621 cggatttccg ccacgccttc gctgctgtgg acgaagcggt cgccgctgac aatcagccct
     4681 tgttctaccg ccgcgccttc aaacgtccgc gccgcccgtt ttgccgtttc aatcaatatg
     4741 ccgtctgaag cgaaccttgc cggcagttgc ggcacttgtc cccaaacata gccgaatgcg
     4801 gttacatcga catcgtggtg cgcgatttct gtgccgacca cgacatcgcc gactttcaaa
     4861 cccttgccca aaccgcccgc gctgccggtg ttgatgacgc agtccggtgc gaattgatgg
     4921 ataagccaag ctgttgcaac cgccgcgttg accttgccga tgccgctcaa tgcaagcacc
     4981 atgcgttttc ccgctaattc gccttcatag gcggcaaact tgccgaaaga gacggctttg
     5041 acattttcca tcatctcgcg caaaagctcg atttcttgtt ccattgcgcc gataacggct
     5101 actgttttca aagacatatt gctgacctgt tgtgaatttc ggatagaatg cctgattata
     5161 cacgctaaca cggcaggatt gagtgggggc ggtttgtccg tgccgtctga aacggtttca
     5221 gacggcacgg cgggtttttg gtagaatggg aaggtacaga ttgtttgaag attaggggac
     5281 gaggatgttt accgatgaaa atatgaccgc aaaggaagaa ctgttcgcat ggctgcgcca
     5341 tatgaaccaa aacaaaggtt ccgacctgtt cgtgacgact catttcccgc ccgcaatgaa
     5401 gctggacggc aaaatcaccc gcatcacgga cgaaccgctg acggcggaaa aatgtatgga
     5461 aatcgccttt tcgattatga gtgcgaagca ggcggaagaa ttttcatcga ccaacgagtg
     5521 caacttcgcc atcagtctgc cggacaccag ccgcttccgc gtcaatgcga tgatacagcg
     5581 cggcgcgacg gcgttggtat tccgtgcgat taccagcaag attcccaagt ttgaaagcct
     5641 gaacctgccg ccggtcttga aggatgtcgc gctgaaaaaa cgcgggctgg ttatttttgt
     5701 cggcggcacc ggctcgggca aatcgacttc gcttgcctcg cttatcgatt accgcaatga
     5761 aaattcgttc ggacacatca tcaccatcga agacccgatc gagtttgtcc acgaacacaa
     5821 aaactgcatc atcacccagc gcgaggtcgg cgtggatacg gaaaactgga tggcggcgtt
     5881 gaaaaacacg ctgcgtcagg cgccggatgt catccttatc ggcgaaatcc gcgaccgcga
     5941 aacgatggac tacgccattg cctttgccga aacggggcat ttgtgtatgg cgacgctgca
     6001 cgccaacagc accaatcagg cactcgaccg catcatcaac tttttccccg aggagcggcg
     6061 cgaacaattg ctgacggatt tgtcgctcaa ccttcaggcg tttatttcgc aacgcctcgt
     6121 tccgcgagac ggcggcaagg gcagggtggc ggcagtcgag gtgctgctca attcgcccct
     6181 gatttcggag ttgattcaca acggcaacat ccatgaaatc aaagaagtga tgaaaaaatc
     6241 cactaccctg ggtatgcaga ccttcgatca acacctttac caattgtatg aaaaaggcga
     6301 tatttccctg caagaagcat tgaaaaatgc cgattccgca cacgatttgc gtttggcggt
     6361 acagttgcgc agccgccgcg cgcaaagttc cagccccgat ttggaactgc tctgatggcg
     6421 gtatggattt ccggacggat ggtttgaaat gatttatccg tggcataatg agcaatggcg
     6481 gcagattgcg gaacattggg agcgtcgtcc caatgcatgg ctgtttgccg gcaaaaaaga
     6541 tacggggaaa actacatttg cccgctttgc ggcgaaggca ctgttgtgcg aaacccctgc
     6601 accgggctgc aaaccctgtg gcgaatgtat gtcctgccat ctgtttggac agggaagcca
     6661 tccggatttt tacgaaatca cccccttgtc ggacgaatcc gaaaacgggc gcaaactgtt
     6721 gcagattaaa atcgatgccg tcaggaaaat tatcgataac gtgtatctga cttcggtacg
     6781 gggcggtttg cgcgtggtac tcatccatcc tgcggaaagt atgaatgtcc aggccgccaa
     6841 cagtttgttg aaagtgttgg aagaaccgcc gccccaagtg gtctttttgc tggtcagcca
     6901 cgcggcggac aaggttttac cgaccattaa aagccgctgc cggaagatgg ttttgcccgc
     6961 gccttcccat gaagaggcat tggcatatct gcgcgacagg ggtgtggcgg agccggaaga
     7021 atttttggcg ttccattcgg gtgcgccgct gtttgatgag gcggacggtg tccgtgcgtt
     7081 gcggattaaa ctgttggata ttttggcaga accaaggttg ttgaagattt tggattacgc
     7141 cgcgcttttc gataaggaga aacttccgct cgacgtattt gtcgggtgga tgcagaaatg
     7201 gctggtcgat ttgggattgt gcctgcaaca catgaaaccc gtctattatc ccgcttatga
     7261 agacaggctg tttcagacgg catccggttt ccgcccgcgc aatgtatttg cggcggagga
     7321 tatgctcaaa cagcttgccc cttacgggtt tcatacttta aatgtcaaaa tgcagattga
     7381 gcatctgctc atcaattatt tggaattgaa gaaagagaac gggtgaatta tgtcagacgg
     7441 acaaaatatt ccggcaaaaa tgatgtcgtt gcagctgaaa gacatgaatc tgctgtacag
     7501 ctcctacatg ccgtttttgg aacacggcgg tctgtttgtg cagaccaacg acgtattttc
     7561 catcggggac gatattctgc ttgccgtaga aatcctcaac ttccccaaac tgttcctgcc
     7621 gaccaaagtc gcctggatca atcctgcgcg tacttcctcc aaacccaaag gggtggggct
     7681 ggcattcaca aaacacgaaa actgcctgaa agtcaaagac cagatcgaag tcgaactggg
     7741 caacacaatc ggcggcagca gacctacgtt taccatgtaa cgccatgcat atcatcgatt
     7801 cgcactgcca cctcaatttt gaaggtttga aagaacgcct gcccgaagtt ttgtccaaca
     7861 tggaagcaaa cggcgtgggg caggcactcg ccatcagcgt cagtagggaa agcttctccg
     7921 aagtctttgc catcgccgaa gcgcacgaac acatctattg caccataggc gtacatcccg
     7981 acagcaagga agccgaagaa ttttctattg cggaaatggt tgccgcagcc gcctcgccga
     8041 aagtggtcgg cattggcgag acgggcttgg attattactg gtgcaaaggc gatttgtcct
     8101 ggcagcacaa acgctttgca gaccacatcg aagcagccaa tcaaaccgga ctgcccgtta
     8161 tcgtccatac gcgtgatgcg gcggcggaca ccttgtctat cctgaaagaa tgccgggtta
     8221 attcgggcgt tatccactgt ttttccgaag acatcggttt tgcccgtgca gcaatggatt
     8281 tggggcttta tatttctttc tcgggaatcg ttacctttaa aaacgcaccc ttggttcagg
     8341 aggcggcaaa atacgttccg gacgaccgca ttttggtgga aaccgatgcg ccgttcctcg
     8401 cccccgttcc caaacgcggc aggcagaacg aaccggcttt tgtgcgccat accgccgaac
     8461 atatcgccaa attgcggaac caaacattgg aacaggttgc ggcatatacg acggaaaact
     8521 tttaccggct gtttaaaaaa gtacccgata tgcgggccgt ctgaccctgt accgacgata
     8581 aggaaaacca tgaaggcaat tcatccgtat gcatgtccgc gctgctgccg gctgcctgcc
     8641 aacacgtttc ggacaggcat ggcaaattcc gcttccaaat tctgcattgc caaaggcggc
     8701 agacgggaag caaaaaagac gaaagcggcg gcggatatgc cccgtgccat ttgccggaca
     8761 gcaggattgt cgaggagtgg gaatatttcc gttcacaaca ttgatactgc gcgatatacg
     8821 gcaaatattg tgggaagttt ccgcttttgc gtataatgcg ccctacctga caaattttgt
     8881 caactttatc aaaaggaata agcgatggct tccatccacg accaaattaa agaagtagta
     8941 acgacacacc gcgtcgtatt gtttatgaaa ggtacgaagc agtttccgca atgcggtttc
     9001 tcttcccgcg ccgtgcaaat cctgaacgcg gcaggctgca ccgattacgt taccgtcaac
     9061 gtattggaaa atcccgaagt gcgccaaggc attaaggaat acagcgactg gccgaccatc
     9121 ccccaacttt atgtgaacgg cgagtttgtc ggcggttcgg acatcctgat ggaaatgtat
     9181 gaggcaggcg agctgcaaga gctgctgaaa gcctgatgga ttcggcaatg ccgtctgaac
     9241 gtgtttcaga cggcattttc ttttccggca aatcaaaaaa aagtataatg gcgcgtctca
     9301 aaatcacact ggaacaccgc gatgaacgtt aatgttatca accatccgct cgtccgccac
     9361 aaattaaccc tgatgaggga ggcggattgc agcacctaca aattccggac gcttgccatc
     9421 gagctggcgc gcctgatggc atacgaggca agccgtgatt ttgaaatcga aaaatacctt
     9481 atcgacggat ggtgcggtca gattgaaggc gaccgcatca agggcaaaac attgaccgtc
     9541 gttcccatac tgcgtgcagg tttgggtatg cttgacggcg tactcgacct gattccgact
     9601 gccaaaatca gtgtagtcgg actgcagcgc gacgaggaaa cgctgaaacc cgtttcctac
     9661 ttcgagaaat tcgtggacag catggacgaa cgtcctgccc tgattatcga cccgatgctg
     9721 gcgacgggcg gttcgatggt tgccaccatc gaccttttga aagccaaggg ctgcaaaaac
     9781 atcaaggcac tggtgctggt tgccgcgccc gagggtgtga aggcggtcaa cgacgcgcat
     9841 cccgacgtta cgatttacac cgccgcgctg gacagccatt tgaacgagaa cggctacatc
     9901 atcccgggct tgggcgatgc gggcgacaag attttcggta cgcgataact gcctaattct
     9961 cggagttgat atgaattttc aagattatct cgccacattc ccgcctatcg atcatttgac
    10021 cggtctggac gttcaggatg ccggcggcaa aacggttcac catattcccg ccgtacaggg
    10081 caagctcggt tcgctcaagc tgtacaacgc tttggcggaa cgttttgacg gcaggttgga
    10141 taaagaagcg gcagaacagg gtttgatatg gtttgtcgaa cacgttgccg acgcgtgcgc
    10201 ccatcccggc aaacatccca atatcgacct gcttgaaaaa gtcgtgcaaa gcggcgaaac
    10261 ctggctgctc aaaccgcttg ccgcccgata attttcgact atgccgtctg aaattcgttt
    10321 cagacggcat tttgtcggaa agaagactgt aaaacgggta ttgccctatg tgttgcccca
    10381 atttggaaga ggttacaccc tttcaaataa aatctgatgc tgccgccacg aaggacggat
    10441 gtccaagtgg cggggtttta accattaagg aaatacgatg aaaaaaacgt tcctttctgc
    10501 cgtattgctt ctgtcggctg cgacccaaac cgtgtgggca gatacggtgt tttcctgtaa
    10561 aacggacaac aacaaatata tagaagttca aaaaatcaac cgcaatcttt acgaatattc
    10621 gttcggcagt gcggcaaaaa aagaaattgc catacgcaac agcaaagctg acctgttggg
    10681 gcgttccgac aggtggcaag gcatgggtag cggacgccgg gcaacgatga aattccaaaa
    10741 cggcgaattt atgtacacca tatggacagg cttcgattcc gtgactcata cggaaagcag
    10801 cggtgtcgtt gtggagcgta ggggcaagga agtcgcacgg gtcggctgta cgccgaaaac
    10861 cgcgcaggcg aatttcaacg atgacgattt tgcctggtaa tcggggcgga taaggcgatg
    10921 gaaacagcga aacccgtcat gctgattgtc cgtccgtcgg gtagggcgaa ggacgatgtg
    10981 gaaacctgcc gtcgtgccgg atggcaggcg gaaatgttga gtccgattga aatcgaaacc
    11041 gacaaagcaa gcctgaaacg gctgccggaa atgtatgccc gtgcggatgc cgtgttttgg
    11101 gtcagtccga ccgccgttga aaccgccgtc ccgtacctta acctttcaga cggcataaag
    11161 gcgcagattg ccgtagggca gggcagccgc cgcgcattgg aacgctgttt ggtcagaacg
    11221 gtcatcgcgc ctgatgacgg caacgacagc gaggcggttt tgcgcctgac ggtttggaac
    11281 agtctgcccg aaggtgcgcg cgtattgttt gtgcgcggac acggcgggcg ggattttctg
    11341 atgaatgcct tgcaggagaa aggttttcgg acggaggtgg cagaagtcta tttcagacgg
    11401 cataaacctt tgaactttca aaatttccaa accgaaaata ttgccgccgc ctatattacg
    11461 tccacagaac tcgtgcgctt gctgttcgga cagcttccgc cgcaattttc ccgattcttc
    11521 aaatccttgc tatactttac ccatcatccg cgcattgcgg aggcattgaa gcgcgaaggc
    11581 gtgtgttcgg tcgaaaccgt ccctacgctg gaagccgcgc tttcccattc ttccatttcc
    11641 gtttcagacg gcatggtctt tcccggaacc tcaaattaat aaggagcaaa acggtgggcg
    11701 aacctgaaaa caaatcatcc gaacccgtac gcgagataca ggcatcaaaa gaaatgccgt
    11761 ctgaaacctc ttccccacgc aaagaaaacg aaacagaagt acacattcct gccgctcctt
    11821 ttatcgtcaa acagtccggc agcaacgctt tggcagtctg cgccctggta ttggcggcat
    11881 tgggtttggg tacaagtggt tttttgtttg tccaaggaca gaatgtcttg aaaaaccaag
    11941 agttggcatt caaccaaaaa atcgacaaag ccgctttggg cgagtcggaa aacgccgccc
    12001 tgctcaaaga caacctcaac cggcaagccg ccatacaatc agagctcgac cgtttggacg
    12061 gaaacgtcaa agcaaacggc gaacaaatct tggaaatgca aaaatcctat cgcgagttga
    12121 ccaaaggacg tgccgattgg ctggtggacg aaaccgatac catactcaat ttggcagcgc
    12181 aacagctggt gttgaccggc aatatccaaa cggcagtcgg cgtgttggag catatcgaca
    12241 gccgcctgtc ccgtttcaat caggcagagc ttctgccgat caagcaggcg gtcagcagcg
    12301 acttggcgga actgaaaaac cgtccctatg tcgatatttc cggcacggca ttgcgcctcg
    12361 acaggctgga aaccgccgta tccggactgc cgctgatgct cgacggcgta ttgaaaccgg
    12421 gcgtacaggt gaagaacgaa gccgcttctg cttcatggtg gcagaacgta tgggaaaaat
    12481 ccctcggcac attgaagggg ctggtcgaaa tccgccgttt ggaaaacaac gatgccatgc
    12541 tgatttctcc cgaacaggca tattttgtgc gtgaaaacct gcgcctccgc cttttggatg
    12601 cgcggactgc attaatgcag cgcaacagcg aagtctatca aagcgatttg aacaatgccg
    12661 aaaccgccgt cagacagtat tttgatgcca agtctcccgc cacgcagtcg tggctgaaag
    12721 aactggcgga attgaaggcg ttggatgtgc gcatgacggc ggatgacggt ttgaaaaaca
    12781 gcctaaatgc cgtccgcgcc tatcgcgacg gtacgcgcat gacggcggcg gaaaatcaag
    12841 aagcggaaca ggcggcttcc gaaccggcaa acgaaaacac agcttccgaa ccggctgccg
    12901 catcggatgt gaagaccata gaagcaccgt ccctgccttc ggaacgcaaa ccggaacagc
    12961 ctgcaaaaaa acagaccgta ccggaaaagg cagggcgttc gccgtccgct aaaggagaac
    13021 gcgcatgaaa acggtagtct ggattgtcgt cctgtttgcc gccgccgtcg gactggcgct
    13081 ggcttcgggc atttacaccg gcgacgtgta tatcgtactc ggacagacca tgctcagaat
    13141 caacctgcac gcctttgtgt taggttcgct gattgccgtc gtggtgtggt atttcttgtt
    13201 taaattcatt atcggcgtat tcaatatccc cgaaaagatg cagcgtttcg gttcggcgcg
    13261 taaaggccgc aaggccgcgc ttgctttgaa caaggcgggt ttggcgtatt ttgaagggcg
    13321 ttttgaaaag gcggaacttg aagcctcgcg cgtgttgggc aacaaagagg cgggcgacaa
    13381 ccggactttg gcattgatgc tgggtgcgca cgccgccgga cagatggaaa acatcgagct
    13441 gcgcgaccgt tatcttgcgg aaatcgccaa actgccggaa aaacagcagc tttcccgtta
    13501 tcttttgttg gcggaatcgg cgttgaaccg gcgcgattac gaagcggcgg aagccaatct
    13561 tcatgcggcg gcgaagatga atgccaacct tacgcgcctc gtgcgtctgc aacttcgtta
    13621 cgctttcgac aggggcgacg cgttgcaggt tctggcaaaa accgaaaaac tttctaaggc
    13681 gggcgcgttg ggcaaatcgg aaatggaacg gtatcaaaat tgggcatacc gacggctgct
    13741 ggcggatgcc gacgatgccg ccgctttaaa aacctgcctg aagcggattc ccgacagcct
    13801 caaaaacggg gaattgagcg tatcggttgc ggaaaagtac gaacgtttgg gactgtatgc
    13861 cgatgcggtc aaatgggtta aacagcatta tccgcacaac cgccgccccg agcttttgga
    13921 agcctttgtc gaaagcgtgc gctttttggg cgaacgcgat cagcagaaag ccatcgattt
    13981 tgccgatgct tggctgaaag aacagcccga taacgcgctt ctgctgatgt atctcggtcg
    14041 gctcgcctac agccgcaaac tttggggtaa ggcaaaaggc taccttgaag cgagcattgc
    14101 attaaagccg agtatttctg cgcgtttggt tctggcaaag gttttcgacg aaatcggaga
    14161 aacgcagaag gcggaggcgc agcgcagctt ggttttggca agtgttgcag aggaaaaccg
    14221 cccttccgcc gaaacccatt gaaaccttca gacggcatag ttgaaatgcc gtctgaaaat
    14281 cattccatac actttacaaa catactgaca gaaagccccg catgactctt ttaaaaaacg
    14341 ataccttcct ccgcgccctg ctcaaacagc ccgtcgaata cacgccgatt tggatgatgc
    14401 gccaggcggg gcgttatctg cccgaataca aagccacgcg cgcgaaagcg ggcagctttc
    14461 ttgatttgtg caaaaacacc gaattggcga ccgaagtcac catccaacct ttggaacgct
    14521 tcgatttgga cgcggcgatt ttgttctccg acatcctgac cgtccccgac gcaatgggct
    14581 tggggctgta ttttgccgaa ggcgaaggcc cgaaattcaa acgcgccctg caacacgagg
    14641 ccgacatcgc caagctgcac gttcccgata tggaaaaact gcaatacgtt ttcgacgcgg
    14701 taacttccat ccgtaaagca ttggacggcc gcgtaccgct tatcggcttc tccggcagtc
    14761 cgttcacgct cgcctgctac atggtcgaag gcggcagcag caaagaattc cgcaccatta
    14821 aaaccatgat gtactcgcgc cccgatttgc tgcacaaaat cctcgatacc aatgcccaag
    14881 ccgttaccgc ctacctcaat gcccaaatcg acgcgggcgc gcaggcggtg cagattttcg
    14941 acacttgggg cggcgtgttg agcgatgcgg cgtttaaaga attcagcctc aaatacatcc
    15001 gccaaatcgt tgccggattg aaacgcgaaa gcgaaggcag acgcgttccc gtcatcgtgt
    15061 ttgccaaagg cggcgggctg tggctggaaa gcatggccca aatcggcgca gatgcattgg
    15121 gcttggactg gacgtgcaac ataggcgaag cacgccgccg cgtcggcaac caagtcgccc
    15181 tgcaaggcaa cttcgacccg ttcgccctct tcggtacgcc ggaatccatc cgcaccgaag
    15241 tcgcacgtat cctggtcgac tatggacaag gcagcggcca tgtcttcaac ctcggacacg
    15301 gcatcaacca acacgccgac cccgaacacg ccaaaatctt agtcgacacc gtacacgagt
    15361 tgtcgcggca gtatcacggc gggtaagccg gcaggaaact gcccgatatg ccgtctgaag
    15421 aatcggaagt tttcagacgg catatgcttc attttgcact ttggtacacc aagccattgt
    15481 gtggtagctt gatattgggt aaccaaaaat cttttccctg ccaacctttg tctttatttc
    15541 ctactacttg ataaagcgta aggaataaat cattgggata tttttctcct aatttacggt
    15601 cgtcttccga aagcattgtg ccggttgctc gagaaagttt acgtccggtt ttgatcaaaa
    15661 cataggtttt aaaacttgga cgttgtgctt ttaatgcttc tacaccagca ataaattttt
    15721 ccttattcca gtcatctgga acataacgac ccaatttatc tagaaccaag aataaatctt
    15781 ctatatctat ttgatataaa tcagattgaa tttcatcttt caaaatagaa ggcaaaattt
    15841 tgttaatctc cgataaatta tcttcgtttg ggtgaagtgg aaaataattc acaccaccca
    15901 caatttgatt gatactatcg aatttcaata cattttttcg agttggattg atatttttcg
    15961 gataaataac ctgaatattg ccgcctgaat tttttgcctg cccaataatc agattatttg
    16021 cttgattgag ttgtacaaag aagtaataga catcaaacgg aatataaagc cgtaataatg
    16081 atttatcgcg gtcatatcca aaaatacgcg aatgttgcca gaaagtatct gcattcggtt
    16141 ttttggcagt gcgactataa taaaccgttt gcagtttcgg aatagttaaa cctcgtccga
    16201 tcacatttcc accaataata atattaaaac ctctttccaa atcaaaatct gattctgtct
    16261 gcgagttaac gacgagagta gagatttgtt tattctccaa aagtgctgtc aatttttcgt
    16321 atatttcatc aaagtgatga atatcgggct tggtcttttg caaatccaaa taactttctt
    16381 taaagctacc tgcaaggtct tccccattat tgactgcttg cactaaatca tttaaaaagg
    16441 cttggatctt ctttgaaaag gcttgatgat cttgaatttt atagcttgga tgtagtgcaa
    16501 aattacagtt agccttatca catagcgcga attctgcaca ggttattaga aaactaagca
    16561 atgcctgttt tgctccttcg gcaatttcac cactctcgtc tttcatatca tccaattcgc
    16621 tatcaataaa acgaactata tagcttggtg gatcagaaaa gacaaagttg ccgccgatgt
    16681 atttttcgcc tgcttcaaag aagtggataa attcaggttg ccaatcagat gcttcgtttt
    16741 gcaacaaaag cgattgaggt gtggcggtca actgaataaa tagactttga caacaggaat
    16801 tcttaatgtc atttagcagt ttattgatag tgctggcatc tttatccggc ttatcagtat
    16861 tagtatttag actggcagca tcggcttcat catccacaat aactaaagga tagcctttta
    16921 ggctactttg actggcaaac aaattacgcc aacgtttcaa tacacgggca ttctttttaa
    16981 tcacgaccaa gatgggattt tctgccttta ttacttccat aaaacttcgg tcgtcagctt
    17041 cagacaatac gataaagttt ttcagattgg ctttagctcg tttgactgtt tgatcctgta
    17101 aatctacgct gtcagtagta agatacaaaa aaactttgtg gtcaccatca tcagccagcg
    17161 cacttaatat acccaaaacc tgtgccgttt tgccactttg aacattgccc agtaataaaa
    17221 cattttgggc gattttcggc tcggtttgtg ataatttctc cataaaacca ttaacagtat
    17281 tttcaacaga gtcagctaat tcgggagggg ttaattgatt caagtaggtt cgcaacattt
    17341 aatttttccc cttaaatgag agcagccaaa catcaggatt gtcggttgaa cggaactcaa
    17401 aatgatcgtt accgtattgg cgtaaggttt cttctgttac aggttcacct gtttttaaac
    17461 agccggttgc ttccaaacgt ccttttaccc attttccaaa aatagtttgg tcaccttttg
    17521 aactgaaatt tttaccattt tgtcctgtcg tattacattc aaactgccag ccatcatctg
    17581 taataactgt aaagtttttt tcctttggat aaccctctgc agttaaaaat cttgttgaaa
    17641 caatcaactc tgcctcatac caaggtcgag gtaaataaaa gccccgttga ttttttctac
    17701 cttccgcaaa aaaacgattt aaactgcttc taggttcagt tttaacaggt aattcaaaat
    17761 aatattgaga agtttgagta aatagttttt ctactctttc tttacttatt ttctcaacac
    17821 ctaaacattg ctctaaatga acattgtgtt ctttaaactc tactggctta tctgctttgt
    17881 tgataggaat tccaagctgc ttaatagttt gagcaatatc ttcccgcaat ttagacgcta
    17941 tttgataatc atccaaatgc accatcgttt cataagttcg ttcggcttta tcccaaaaac
    18001 aagtcagatt tgccgaaccg attagtgtat ggatttcccg ctgattttga aatgaataca
    18061 ttttcccgtg aaactttacc cacggcgaca cataaaccat tccacgctgt tcatcttgca
    18121 aaaagtcgtt caattcgcat aagctgctgt attgtgggcg actgaaacct tctaaatagt
    18181 gcattccgac catcaaactg agtttttgtt tatgatcgct ttcttccaaa attttatgca
    18241 gttcttttac tgcatcattg gatacataac ctgtcgccat caacatatca tcggcagaca
    18301 taaacaaatc catccaaacc ttattcaagg atttatctgt gattttggca ttggcaatgt
    18361 tggaaaaaac cgtattcatc gttttccccc acggattttg ttcagtctgt cttggaagaa
    18421 gacgtttttt tctgcttcta atttttcatg tagatttacc ttgtgataac cgtctgaaaa
    18481 aactggaagt atggcttttg ctagttctac tacaccctgt ggaggaacgg cgtttccgat
    18541 ttgacggcgg acttccgtgg ttgagcccat aaactcaaaa ttatcgggaa aactctgtaa
    18601 tcttgcccgt tcgcggttgg taaaggcacg cggttcaggg aagtgataac cccaagtacc
    18661 tccaccgcca gcagcaatga tggttgtaga aggtttgtcc cgatgcattc gtcggtaaac
    18721 caaactcaac atacttttaa cgtataaagg gtgatctcta ggaatgtcgc tgtaatttcc
    18781 tccttctgga atcagattca accgtttttt agtttgttca ctgattttta actgctcgtg
    18841 attgtttgcg ttttcaggaa tatcagaaat agcctgacca gcggttacat aaggtaaaag
    18901 accgtaatcc gtattttcac cgtgagtagg ctcagggtgg tggaagtcga aacccgtatc
    18961 caaacgcaca ccgacaatta gcacacgttc acgaaattga ggtacgccaa attcagcaaa
    19021 gttatacagc ttcgcctgaa cgtaataacc gcaattttca aagtcggtaa taatttgctg
    19081 gatggctttt ttcttgttag cagtcaataa acctttcaca ttctcagcaa caaaaacttt
    19141 cggttttttt gcatttacaa aacgtaaaaa gcttttataa agattgccgc gctcaccctc
    19201 taagcccggc tgtttccaaa tcatggaaaa atcttgacaa gggaatccgc ctaaaatgat
    19261 gtcgcaatcg ggaatagttg gattattcgg atcaatttgt tcaatatcac cttctacgat
    19321 gacatcgccg atatttttac ggaaactttc gcaagcccaa tgggagaaat cgttcgccca
    19381 aacagtttca taaccagctt gatgaaagcc caaatccaaa ccgccacatc ctgaaaatag
    19441 agataggatt tttggtttat tgctagaaga attaagattt gagatttgtt tagatagcat
    19501 aggatttcac tcctatgcat aaatttgata aaaatccttg tatggatttt aaaggtgaac
    19561 tatgctct
//
LOCUS       DAWFJW010000029        19434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFJW010000029 DAWFJW010000000
VERSION     DAWFJW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.029"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            118..747
                     /locus_tag="WGR19_000835"
     CDS             118..747
                     /locus_tag="WGR19_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0798387.1"
                     /translation="MIQNVVTSIILYFGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNRNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILIGNNSFDMLWTVSGQEKIL"
     gene            893..2569
                     /locus_tag="WGR19_000836"
     CDS             893..2569
                     /locus_tag="WGR19_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0798388.1"
                     /translation="MLTYTPSDARTPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            2671..3276
                     /locus_tag="WGR19_000837"
     CDS             2671..3276
                     /locus_tag="WGR19_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0798389.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3348..3740
                     /locus_tag="WGR19_000838"
     CDS             3348..3740
                     /locus_tag="WGR19_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0798390.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            3745..3987
                     /locus_tag="WGR19_000839"
     CDS             3745..3987
                     /locus_tag="WGR19_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798391.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(4065..4511)
                     /locus_tag="WGR19_000840"
     CDS             complement(4065..4511)
                     /locus_tag="WGR19_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0798392.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(4643..4855)
                     /locus_tag="WGR19_000841"
     CDS             complement(4643..4855)
                     /locus_tag="WGR19_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0798393.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5144..6994)
                     /locus_tag="WGR19_000842"
     CDS             complement(5144..6994)
                     /locus_tag="WGR19_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0798394.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            7525..8262
                     /locus_tag="WGR19_000843"
     CDS             7525..8262
                     /locus_tag="WGR19_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0798395.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKERVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            8264..8950
                     /locus_tag="WGR19_000844"
     CDS             8264..8950
                     /locus_tag="WGR19_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0798396.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPIA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPLAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NTLARKLDKR"
     gene            9109..9972
                     /locus_tag="WGR19_000845"
     CDS             9109..9972
                     /locus_tag="WGR19_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798397.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(10034..10603)
                     /locus_tag="WGR19_000846"
     CDS             complement(10034..10603)
                     /locus_tag="WGR19_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0798398.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETDYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            10762..11622
                     /locus_tag="WGR19_000847"
     CDS             10762..11622
                     /locus_tag="WGR19_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0798399.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     VALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            11757..12095
                     /locus_tag="WGR19_000848"
     CDS             11757..12095
                     /locus_tag="WGR19_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798400.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNESIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            12463..12816
                     /locus_tag="WGR19_000849"
     CDS             12463..12816
                     /locus_tag="WGR19_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0798401.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            12806..13672
                     /gene="atpB"
                     /locus_tag="WGR19_000850"
     CDS             12806..13672
                     /gene="atpB"
                     /locus_tag="WGR19_000850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0798402.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            13729..13965
                     /gene="atpE"
                     /locus_tag="WGR19_000851"
     CDS             13729..13965
                     /gene="atpE"
                     /locus_tag="WGR19_000851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0798403.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            14036..14506
                     /locus_tag="WGR19_000852"
     CDS             14036..14506
                     /locus_tag="WGR19_000852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0798404.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            14511..15044
                     /locus_tag="WGR19_000853"
     CDS             14511..15044
                     /locus_tag="WGR19_000853"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0798405.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            15055..16602
                     /locus_tag="WGR19_000854"
     CDS             15055..16602
                     /locus_tag="WGR19_000854"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0798406.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            16627..17502
                     /gene="atpG"
                     /locus_tag="WGR19_000855"
     CDS             16627..17502
                     /gene="atpG"
                     /locus_tag="WGR19_000855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0798407.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            17541..18938
                     /gene="atpD"
                     /locus_tag="WGR19_000856"
     CDS             17541..18938
                     /gene="atpD"
                     /locus_tag="WGR19_000856"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0798408.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            18949..19371
                     /locus_tag="WGR19_000857"
     CDS             18949..19371
                     /locus_tag="WGR19_000857"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0798409.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         4444 a         5059 c         5118 g         4813 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccactatatt ttgacggggt tgaagttgac
       61 atctacaagg tttaatgttt gaatacatta aaaaaatata tttgaatgag atgttttatg
      121 attcaaaatg tcgttacttc aataatcctg tattttggga cagccgtaga cttacttatt
      181 atcctaatgt tattttttgc caaaagaaaa agcagaaaag acatcattaa catctattta
      241 ggccaatttc taggctctgt tagcctgata ttgctaagtt tgctttttgc atttgtctta
      301 gattatattc ctagtaaaga gattttaggt ttgctcggct tgattccgat tttcctaggc
      361 atcaaagttt tgcttttagg agattccgat ggagaggcta ttgccaaaga gggtttgcgc
      421 aaagataata gaaacctgat ttttctagtc gctatgatta cttttgcaag ttgtggcgct
      481 gacaatattg gtgtctttgt cccatatttt actaccttaa attcagcgaa tttgatagtg
      541 gctttactta cctttctagt catgatttat ctcttggttt tttctgccca aaaattggca
      601 caagtccctt ctgtcggaga aactttggaa aaatatagca gatggtttgt tgccgttgtt
      661 tatttaggat tggggatata tatcctgatt ggaaacaaca gttttgatat gctatggact
      721 gtgtcgggcc aggaaaaaat attatgaaaa agatagtatc tgcaaagact gccccgtgca
      781 gtcttttttg ttgccggtct ttttgtgtct gatgccgtct gaagcagtct ctgcacgacc
      841 tttgtgcgaa tatttgctac acttggcaat ccgtccattt cttctcacgc ttatgctgac
      901 ctataccccg tccgatgccc gaacacctgc caaaacccac gaaaagccgt ggctgctgct
      961 gttgatggcg tttgcctggt tgtggcccgg cgtgttttcc cacgatttgt ggaatcctgc
     1021 cgaacctgcc gtctataccg ccgtcgaagc actggcaggc agccccaccc ccttggttgc
     1081 ccatctgttc gggcatgccg atttcggcat accacccgtg tatctttggg ttgccgccgc
     1141 gttcaaacat ttgctgtcgc cgtgggcagc cgacccgtat gatgccgcac gctttgcagg
     1201 cgtgtttttc gccgttgtcg ggctgacttc ctgcggcttt gctggcttca actttttggg
     1261 cagacaccac gggcgcagcg tcgtcctgat tctcatcggc tgtatcgggc tgattccgac
     1321 cgtacacttt ctcaaccccg ctgccgccgc ctttgccgcc gccggactgg tgctgcacgg
     1381 ttattctttg gctcgccggc gcgtgattgc cgcctctttt ctgctcggta cgggctggac
     1441 gctgatgtcg ttggcagcag cttatccggc agcatttgcc ctgatgctgc ccttgcccgt
     1501 actgatgttt ttccgtccgt ggcaaagcag gcgtttgatg ttgacggcag tcgcctcact
     1561 tgcctttgcc ctgccgctta tgaccgttta cccgctgctc ttggcaaaaa cgcagcccgc
     1621 gctgttcgcg caatggctcg acgatcacgt tttcggtacg ttcggcggcg tgcggcacgt
     1681 tcagacggca ttcagtttgt tttactatct gaaaaacctg ctttggtttg cattgcctgc
     1741 gctgccgctg gcggtttgga cggtttgccg cacgcgcctg ttttcgaccg actgggggat
     1801 tttgggcatt gtgtggatgc ttgccgtttt ggtgctgctt gccgtcaatc cgcagcgttt
     1861 tcaggataac ctcgtctggc tgcttccgcc gcttgccctg ttcggcgcgg cgcaactgga
     1921 cagcctgagg cgcggcgcgg cggcgtttgt caactggttc ggcattatgg cgttcggact
     1981 gtttgccgtg ttcctgtgga cgggcttttt cgccatgaat tacggctggc ccgccaagct
     2041 tgccgaacgc gccgcctact tcagcccgta ttacgttccc gacatcgatc ccattccgat
     2101 ggcggttgcc gtactgttca cacccttatg gctgtgggcg attacccgca aaaacatacg
     2161 cggcaggcag gcggttacca actgggcggc aggcgttacc ctgacctggg ctttgctgat
     2221 gacgctgttc ctgccgtggc tggacgcggc gaaaagccac gcgccggtcg tccggagtat
     2281 ggaggcatcg ctttccccgg aattgaaacg ggagctttca gacggcatcg agtgtatcgg
     2341 cataggcggc ggcgacctgc acacgcggat tgtttggacg cagtacggca cattgccgca
     2401 ccgcgtcggc gatgtacaat gccgctaccg tatcgtccgc ttgccccaaa atgcggatgc
     2461 gtcgcaaggc tggcagacgg tctggcaggg tgcgcgcccg cgcaacaaag acagtaagtt
     2521 cgcactgata cggaaaatcg gggaaaatat attaaaaaca acagattgag ccgaatttct
     2581 ggattaagtg ccggaaatcg cgtataattg cgcgattaaa cctttataaa tcagccgttt
     2641 tacaggcatc acacaggagc gacaattatt atgatgaccc tctattccgg cattacctgc
     2701 cccttcagcc accgctgccg cttcgttttg tacgaaaaag gtatggattt tgaaatcaaa
     2761 gacgtcgata tttacaacaa gcccgaagac ctcgccgtca tgaacccgta caaccaggtc
     2821 cccgtactgg tcgagcgcga tttggtgctg cacgagtcca acatcatcaa cgaatacatc
     2881 gacgaacgct tccctcatcc gcagcttatg cccggcgatc ccgttatgcg cggcaggggc
     2941 cggctggtgc tgtaccgtat ggaaaaagaa ttgttcaacc atgtccaagt tttggaaaac
     3001 cccgccgcca ccaacaagga acaggcaaaa gcgcgcgaag ccatcggcaa cggtctgacc
     3061 atgcttgccc cttcgttcag caaaagcaaa tacatcctcg gcgaagattt ttctatgatt
     3121 gatgtcgccc ttgctccgct gctgtggcgg ctcgaccact acgatgtcaa actgggcaaa
     3181 agtgccgcgc cgctgctcaa atacgccgag cgcatcttcc agcgcgaagc ctttatcgaa
     3241 gcactgacac ccgccgaaaa agccatgcgc aaataagtcc gaaatgcttg caaaacccgc
     3301 cgttttgcag gcattttcac attcaggcac accacacgga acccatcatg cccacctcaa
     3361 ccaaacccta catcctccgc gcactttgcg aatggtgcag cgacaacagc ctcacaccgc
     3421 acatccttgt ctgggtcaac gaacacacgc gcgtccccat gcagtacgtc cgcgacaacg
     3481 aaattatgct caacatcggc gcgaccgcca cgcaaaacct tcaaatcgac aacgattgga
     3541 tcagcttttc cgcccgcttc ggcggacagg cgcacgacat atggatacct gtcggacacg
     3601 tcctcagcct tttcgcacgg gagaccggcg aaggtatggg gtttgaattg gaagcgtatc
     3661 gccccgacac gccgcctgaa aacacctctg ccgaaaccgc gccccggccc gccaaaaaag
     3721 gcttgaaatt ggtcaaataa atctatgccg tctgaacgga atcgtgtttc agacggcatt
     3781 ttgtccgatg gggcgcaaac ggaatccgtt tatcggcaaa acccgtttcg gtgtatcgaa
     3841 accgtgttgc ccctgccgat tcgatattgg gacgttattg catcagcatt agagtgcaat
     3901 tatggtgcag ctaaccaagc gatagagcgg caacgccaac gagcagcggc agttgctgtg
     3961 catgagcgtg ttgcaaaatt tttataaata tcatcaaacc atgaaaatgc cgtctgaacc
     4021 tgcgatacgg gcttcagacg gcattttgtc cgatattcgg gcaatcaggc ggtcagcacg
     4081 gctttcagga ttttgttgac ttcgcccata tcggctttgc cggcgaggcg ggttttcaat
     4141 acgcccatca ctttgcccat atccgccata cctgccgcgc cggtttcggc aacggcggca
     4201 tcgaccgcag tacggatttc cccggcggag agcatttggg gaaggtagcg gtgcagtacc
     4261 tcgatttcgg cgttttcttt gtctgccaaa tcctgacggc cggcttcagt gtagattttc
     4321 gcgctgtctt ttcgctgttt gaccattttg gtcaggatgg cggtgatttt ggcatcgtcg
     4381 gcttcggtgc gttcgtccac ttcaaactgt ttgacggcgg cgttgatgag gcggatggtg
     4441 ccgagggaaa cttggtcttt ggcgcgcatc gcggttttca tgtcttcggt aaggcggatt
     4501 ttcaggctca tgatgtcctc gctgggatgt cggatggaaa cggcgggttt cgatgccgtc
     4561 tgaaaagcaa aacacaccgc agagcaggtc ttgcggtgtg tcctcatatc acagggctga
     4621 cctgtaatct gtacttgaac gtttagtaca ttttgggcgg cagttgttgg ctgcgcaggc
     4681 gtttttgcag gcgttttacg gctgccgctt ttttgcgttt gcgttcggta gtcggttttt
     4741 cgtaggcttc gcgggcgcgc agctcggtca gcaggccggt tttttctacg gcgcgtttga
     4801 aacggcgcat agcgacttca aatggttcat tctcttttac gcggattgca ggcattttat
     4861 ttcctttaat aaattcggtt gtttcatctg cccatcatat cggtggaaag ggtagggcag
     4921 acggtgttga aggtttaccg tatcgctgtg ccttgcggcg gcgggatacg cgccgtgacg
     4981 gaaacatcac ctcctaacgg gtcggcttgt gccgtgtcaa gctgcttgaa aaagcaggaa
     5041 aaacaatttt cggattgtct tatatttatg gttcgccgtc aatgccgttc gggataaaaa
     5101 tgccgtctga aagaccgggc gggtttcaga cggcatcggt acgtcagcgg gcggggacaa
     5161 tgcccatacg ctgtttgagc gggatgtgcg gcgcgccgaa gagggaggcg tagtaggcgg
     5221 cattggccat cacttttttg acatagtcgc gcgtttcgga aaacgggatg gtttcggcat
     5281 ataccgcgcc ttcgaggggc gtgtccgcct gccatcggcg cgccctgccg ggaccggcgt
     5341 tatagcctgc ggtggcgagg acttcgttgt tttgcaggcg gcgtttggtg tccgccatat
     5401 accacgtccc catacggata ttgccgtcgg cggtgtaaag ttgtgcggca tccataccga
     5461 ttttgccggc gatttcgcgc gcggtggcag gcataacctg catcagcccc tgcgcgccta
     5521 cgcgggattg cgcgcctata acgaagcggc tttcctgacg aatcaggccg taaacccaag
     5581 ccggatcgac attaacattt tgcgcgtggc ggattaccgt gtctttaaac ggcgaaatat
     5641 agcgcaaggt gtagttgagt ttgcggtcgg tgcgttccgc gctgttgacc gccatatcgt
     5701 aaaaaccgtg gtcgaacgcg gtttgtgcgg cggtcagcag cttgtcttcg tcgaacccgc
     5761 gtgtggcaaa acgccattcc gcctgagcct gacggcgcat tttcgcatcg ccggcggtcc
     5821 ggctgttttg gaacagtacc agcgcacgct taatcgcgcc gtcttccgcc atacggagga
     5881 cgctgtttgc gtcggcatcg ggaacattgt tgcgcgtatc gattttgcgc cccaactctt
     5941 cccctgccag caccgcataa aaattcctgc ccgtcgctgc cgcctgtttg taaagttttt
     6001 ccgcctcttg cgtgttgccc gttgcggcgc ggctgcgtgc cagccagtag agccaggtcg
     6061 ggcttttttg cagtttttcg ggcatatgcg agataacgga ggccagctcg tcccaacgtc
     6121 gggcgcgcaa ggcggcgcgg gcgtaccact cgatttggtc gtcggtcagt tggcggcggt
     6181 cggcaacctt gccgtaatag tccaaggcgg caggcacatt gaggttttgc gactgataat
     6241 gccccaatac gccccacgcg aaactgcgtt gttcgaggct taaaccgctt tccatttcgg
     6301 acagcagggc ggcggcattc ggcgatttgc gtgcttcttt gccgatgacg ttcaacaggg
     6361 catattcgcg cgaaccttgt gtaccgccgt caaacgggct gcccaatgcg gcggcaaggt
     6421 tgcgtgcgtc tgtggtttgg cggccggcca gcagtccgcg cacgcgcctc caggcgtcgt
     6481 tgccgtccaa caagccggat gcggctgcct gttccaacag tttggtgcag cccgaaggca
     6541 gtttgcccgt atttttgacc agttcggcgg cacgcgtata gtcgttgcgg ctcgaatcgg
     6601 cgtagcattc gacttcttgg gcgcgccctg ccggttcgag tttggcatat tcctgtgcaa
     6661 acagcgtcca ctgtttgcgt gcgcccaaag acttcagcca ctcgttgcgg acattttccg
     6721 ccatcgcact gtcgccggcg ttttccaaat aggcggcgac ggcggcatcg tttttctgtt
     6781 tcactgcatc cagtgcggac gggtagccgc cgtaatctgc cagcgttttt ccttccggtt
     6841 cggcagggcg ggtgggaacg cttgccgaaa ggtcggcagt ttctatattg tctgccgggg
     6901 tcttgccggc tggcagtgtg tttgtcgaag aacacgcggc aagcactagg gtcgccagca
     6961 gcggcaggga atgcttcata gagggtaggt acatcggatt tccttaagaa tcggaaccct
     7021 gaacggtcag ggttggaaaa gacaaaatgc cgtctgaaca ggcgtttgcc cgaattatat
     7081 gccgaaactg caccgccttt ggaatgtttc cgacataatt tatagtggat taaatttaaa
     7141 ccagtacggc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
     7201 caagtgaatc ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt
     7261 ttgttaatcc actatatatt tttcaattat ttgccgtttt ggtgcgaacc gctgcctttg
     7321 cccgtttcag acggcattgt ccgaaatggt tgcctgcttc ctgccttatt gacaaaaaaa
     7381 tgctttcccg ataatatcct acgaaaatta acctgccgat ttgacacagc ttgcgggcat
     7441 aacagctaaa gcgttccgac aattccagct ttatcttccg cgcccgttgt gtccgacatc
     7501 gggctttgtt gtatgggaaa gacaatgatt attttggaca aggtttccaa gcattaccaa
     7561 acgcgcgaca agacccgttt tgccgctgtc gagccgacca gcctcgaaat ccgcgacggt
     7621 gaaatcttcg ggctgatggg ttattcgggt gcaggcaaat ccaccctgtt gcgcctgatt
     7681 aacctgttgg aacgccccga cagcggcaag gtcaacgtct gcggacaaga gctgaccgcg
     7741 ctcgatgccg ccgcattgcg tcaggctcgg cagaatatcg gcatggtgtt tcagcagttc
     7801 aacctgttga acaaccgcac cgttgccggc aacgtcgcct tcccattgga aatcgccgga
     7861 tggccgtctg aaaaaatcaa agaacgcgtt gccgaatgcc tcgaaatcgt cggcttgacc
     7921 gaacgcgccg gccactatcc cgcccagctt tcaggcgggc agaaacagcg tgtcggcatc
     7981 gcccgcgcac tcgcgcccaa accccaagtc atcctcgccg acgaacccac ttccgccctc
     8041 gaccccgcca ccacgcgcag cgtcttggaa tgtttggaag acatcaacaa acgattcaac
     8101 gtaaccatcg tcatcgtaac ccacgaaatg agcgttatca gacgactgtg cgaccgcgcc
     8161 gccctgttgg ataagggaaa agtcgtcgaa atcgtcgaag tacgcggcaa ccaaatccac
     8221 gcccgatccg acatcgggcg cgaactcatc agagaggact gaaatggcag acctgacctt
     8281 ccaacaagcc gtttccaaca tcatcggtat gaaaggcgaa atcgtccgcg cattgggcga
     8341 aaccttcgtg atggtcggct tgtccaccac attcgccgtc gttttcggca cgttgctggg
     8401 cgtgttgctc ttcgtaactt ccagccgcca actgcattac aacaagccgg tgaacttcct
     8461 gctcgacaac ctcgtcaacc tcatgcgcgc cttccccttc gtcattttga tgattgcgat
     8521 gatacccgcc acacgcgcca tcgtcggcag caccatcggc ccgattgccg cctcgctggt
     8581 gttgagcgtg tcgggattgt tttattttgc ccgactggtg gaacaaaacc tgcgcgaagt
     8641 ccccaaaggc gtgattgaag ccgccgccgc gatgggtgcg ccgccgcttg ccatcgtccg
     8701 caaagtcctc ttaaacgaag cgcgcgcggg tatggtttcc agcattaccg tgcttgccat
     8761 cgggcttttg tcatacagcg cggcggcggg catgataggc ggcggcggct tgggcgacct
     8821 cgccatccgc tacggctact accgctacca aaccgaagtc atcgtcttca tcgtcgccct
     8881 cctcgtgctg ctggtcatcc tgattcaaag caccggcaac acgttggcgc ggaaattgga
     8941 taaacgttaa acccgaatgc cgtctgaacg ccaaaacccc caccgctatc cgaaaaatgc
     9001 tataaaatcc ccctgttcgc ggcaaatgcc gtctgaacgc cgaatccgga cggcaggact
     9061 ccctgcccgt catttttgtt tgaaactgcc acaacatcag gagaaaatat gaaaaccttc
     9121 ttcaaaaccc tttccgccgc cgcactcgcg ctcatcctcg ccgcctgcgg cggtcaaaaa
     9181 gacagcgcgc ccgccgcatc cgcttctgcc gccgccgaca acggcgcgga gaaaaaagaa
     9241 atcgtcttcg gcacgaccgt cggcgacttc ggcgatatgg tcaaagaaca aatccaagcc
     9301 gagctggaga aaaaaggcta caccgtcaaa ctggtcgagt ttaccgacta tgtacgcccg
     9361 aatctggcat tggctgaggg cgagttggac atcaacgtct tccaacacaa accctatctt
     9421 gacgacttca aaaaagaaca caatctggac atcaccgaag tcttccaagt gccgaccgcg
     9481 cctttgggac tgtacccggg caagctgaaa tcgctggaag aagtcaaaga cggcagcacc
     9541 gtatccgcgc ccaacgaccc gtccaacttc gcccgcgtct tggtgatgct cgacgaactg
     9601 ggttggatca aactcaaaga cggcatcaat ccgttgaccg catccaaagc ggacatcgcc
     9661 gagaacctga aaaacatcaa aatcgtcgag cttgaagccg cgcaactgcc gcgtagccgc
     9721 gccgacgtgg attttgccgt cgtcaacggc aactacgcca taagcagcgg catgaagctg
     9781 accgaagccc tgttccaaga accgagcttt gcctatgtca actggtctgc cgtcaaaacc
     9841 gccgacaaag acagccaatg gcttaaagac gtaaccgagg cctataactc cgacgcgttc
     9901 aaagcctacg cgcacaaacg cttcgagggc tacaaatccc ctgccgcatg gaatgaaggc
     9961 gcagccaaat aaggcagtcg tataaaatga tgccgtctga actgtatccg tgttcagacg
    10021 gcatttttgt cctttaatcc gccattccct gccattccgc cgaatccggc gtatcgattc
    10081 cgaacagcga caaagcgtgt gcaacactgt gcgccactat gtcgtccgcc gtctgcggtt
    10141 tgcggtacat cgcaggaaca gggggaaaca ccacgccgcc catttccgtt acccgcttca
    10201 tattgtccaa atgggcaagg ttcagcggcg tttcgcgcac catcagcacc agccgccgcc
    10261 tttccttcaa aaccacatcc gccgcacgcg tcagcagatt gtcgccgaag ccgtgcgcga
    10321 cagaggcaag cgtccgcatc gaacaggggg cgaccagcat cccatccgtt ttaaacgtac
    10381 cgctggcaat gcacgccccg atattgccga tcggatgcac gaagtccgcc aaggcatata
    10441 cctcgtctct cgcataatcc gtttccgaag cgcgcgccat ctccgcacct ttcgatacca
    10501 caaggtgcgt ttcgatatct tgcgcgcgta aaagttccaa agccttcacg ccgtattgga
    10561 aaccgctcgc cccactgatg ccgattatca aacgccgtac catcatccgc ctttcccata
    10621 aaaccgcctg caacggcaaa ccggctatta tagtaaaaaa acagaaatcc gataaacacg
    10681 gatacaaatt gtcggcaata cccaatatcc gataaaatat ccgatttaac atcctatctg
    10741 aaataggcac gggagggcgg tatggcaaaa gtaaaaggcg gattggggcg cggtttggat
    10801 tcgctgctcg ccaacggcgc agacaacagc agcggcgacc gattgaccac ggttgcggtt
    10861 aaagatatcc ggcccggccg ctatcaggcg cgtgttcaaa tcgatgacga agccttgcag
    10921 gagctggcag attcgattaa ggcgcaaggc gtgatacagc ccgtcatcgt gcgcgaacac
    10981 ggactgtccc gatacgaact gattgcaggc gaacgccgtt ggcgcgccgc acagattgcc
    11041 ggcctgaccg aaatccccgc cgttatcaaa accatcagcg acgaaaccgc attggcaatg
    11101 ggtttgatcg aaaacctcca gcgcgaaaac ctcaacccca tcgaagaagc acaaggcttg
    11161 aaacgccttg ccgacgagtt cgggctgacc cacgaaacca tcgcccaagc cgtcggtaaa
    11221 agccgaagcg cgatttccaa cagcctgcgc cttttaagcc tgcccgaacc cgtgcaggaa
    11281 atgctttacc aacgccgcct cgaaatgggg cacgcccgcg cattgctgac cctgcccgtc
    11341 gtcgaacagc tcgaattggc gcaaaaggcc gtcaaaaacg gctggtcggt gcgcgaagtc
    11401 gaacgccgca gccaggtcgc ccttcaaaac aaacgtcccg agcccaaaaa gactgccgcc
    11461 gccgacatcg gccgcctgaa tgatttgctg actgaaaaac tgggtgtcaa cgccgaaatc
    11521 aaaaccgcca atcataaaaa aggcagaatc atcctgcatt tcgacacgcc cgaaaccttc
    11581 gaccatatct tgaagcagtt gggcatagat taccgacctt aattttgcgg gatataccgt
    11641 ctgaaatata gagaacagct ttccagattt taagtgggaa atataattct attgacattt
    11701 ttctgcttca cgtaagaatc gttttcctgt tttcattttt aattttcgag gaaattatga
    11761 acacacgcat catcgtttcg gctgcgttcg ttgcgttggc attagcaggt tgcggctcaa
    11821 tcaataatgt aaccgtttcc gaccagaaac ttcaggaacg tgccgcgttt gccttgggcg
    11881 tcagcccaaa tgccgtaaaa atcagcaacc gcagcaatga aagcatacgc atcaacttta
    11941 ccgcaactgt gggtaagcgc gtgagccaat gctatgttac cagtgtaatc agcacaatcg
    12001 gcgttaccac ttccgatgca atttgtttgg gaggcggaac gcacaaaggc aaaagtcaat
    12061 gcaatgcttt gcttaaagcg gcaggccgtt gctaatcctt tattcggaaa aggtcgtctg
    12121 aaaatatttt cagacgacct tttatttatt gagcaaattc cccaaactgc cgacaatccg
    12181 accgataaac tgctacaatt tcgcgttgat tttttattat ttccacgtaa agcaaaccaa
    12241 tcacgtaaga acattaaaaa tgtaaagaaa ttgcggacaa actccttaat atcctgtaat
    12301 tatatgtaat gtgtgttaga atgacacctg tcgccgttat tcaggcggcg agcgcggcgg
    12361 attgggatac gttgtttcac aaagattaac tgtcgcttga cgtaataagg cttctaaatt
    12421 atattagccg cgccccaaat ccgaggcgga aacattccct ttatgaagca gattatcatc
    12481 ctacaatccg ccgtattgtc catatgcgcc gcagttgcct ttgctgtatg gggttttgcc
    12541 ggattccttt cggcggtcgg tggcggtttg tcctacctgc ttcccacgtt tgttgcagtt
    12601 ttacttttaa aacttttcag gggaaacccc ttcctgcaaa gcaggatgtt cgtcttcgga
    12661 gagattttaa aagtagtgct gtcgctgttg tccatgcttg ccgtatttgc aatatggcat
    12721 caatcgctgg tgttcgcccc gtttctgatg gggctgctcg gtgtcagcca tttggttttt
    12781 ttagtattgt tgagagtgaa agattatggc aggtgaaacc attaccgctg ccgactacat
    12841 caagcaccac ttgcagagct tgaccagttt gtcggatgtt actcagggtc agggactgaa
    12901 aaacattgct gatttttcgt ttattaacct tgatgccgtc ttttttgccg tcctgttggg
    12961 cgtaatcggc agctttctgt tgtggcgcgg tgcaaaaaaa gcgacggcag gcgttcccgg
    13021 acgtttccag gccgcagtgg aaatcttgtt tgagtttgtg gacgatatgt gtaagagcat
    13081 cattcacaac gaaaagtcta gaaaagccgt cgcgccgttg ggtctgacgc tgtttgtctg
    13141 gatttttctg atgaacgcga tggatatgct gccggtcgat ttgctgccga tggtatggca
    13201 gggcattacc ggcaaccatc acgccctgct gcgcgtcgta ccgactgccg atttgaacac
    13261 cactttggca ctcgctgtcg gcgtgttgct gatttgtatc tattacaaca tcaaaatcaa
    13321 aggattgggt ggctggtttc acgagttgtt cagcgcgccg ttcggtgcaa aactcgcacc
    13381 tgcgaacttc ctgttgaatt tggtcgagtt tctttctaaa accgtatccc acggtatgcg
    13441 gttgttcggc aatatgtatg cgggcgaatt ggtattcttg ctgatagcct tgcttggcgg
    13501 tgcttgggcg gcttccggca gcgtcgaagt catggatccg attctgtttg tattccacat
    13561 tattgccggt ttggcatggg cgattttcca tattttggtg attaccctgc aggcgtttat
    13621 tttcatggcg ttggcgttcg tctatatcgg acaggcacat gatgcgcact gattttccta
    13681 agtagtttgt ttttgtagtt taacctttgt ttctttaagg agtttaaaat gggtttgatt
    13741 gctatcgcat gtggtttgat cgttgcattg ggtgcattgg gtgcctctat cggtatcgca
    13801 atggtcggtt ctaaatattt ggagtcttct gcccgtcagc ccgaactgat cggtccgctg
    13861 caaaccaaac tgttcctgat tgccggtttg attgatgccg cattcttgat tggtgtcgcc
    13921 attgcactgc tgttcgcctt cgttaacccg tttgcaggtg cataatcagg acggattcgt
    13981 ccgttccgta caggcgcagc ctgtttgatt aaccccagta cgaaggttaa gtaacgtgaa
    14041 tatcaatgca acattattcg ctcaaatcat cgtctttttc ggtttggtat ggtttaccat
    14101 gaaattcgtg tggccgccga ttgcaaaagc tttggatgag cgtgccgcaa aagtcgccga
    14161 aggcttggct gccgccgagc gtggtaaaag cgatttcgag caggctgaaa aaaaggttgc
    14221 agaactcttg gcagaagggc gtaatcaggt ttccgaaatg gttgccaacg ccgaaaaacg
    14281 tgccgccaaa attgtcgaag aagccaaaga acaggcttct tccgaggcgg cgcgcattgc
    14341 agctcaggca aaggccgatg tggagcagga attgttccgc gcacgcgaat ccctgcgcga
    14401 gcaggttgcc gtgttggctg tcaaaggtgc cgaatctatt ttgcgcagcg aagtcgatgc
    14461 ttccaaacac gcaaaactgc tcgataccct gaaacaggag ctgtaatctt atggcagagt
    14521 tcgcaacgat tgccagacct tatgcaaagg cattgttcgg tctggctcag gaaaaaagcc
    14581 aaattgagtc ttggttgggc ggactggaaa aacttgcggc ggttgttcag gaagggaagg
    14641 tggcttcatt gattgaccgt cctgaaacga atgcttcaga aaaagcagat atcctcatcg
    14701 atttggtcgg tttgaaagac aaggagttga aaaactttgt tatcgtcttg gccgggcaga
    14761 aacgtttgtc gatattgccg gaagtatatg ctcaatatca agacttgacc ttatcattca
    14821 accatatcaa atctgccgtc atttacagtg cctatccgtt gaccgacaaa caggtcggcg
    14881 agttggcgca aatgctgaat aagcgtttcg acagcgagct gaaaatctct gtcgaaatcg
    14941 aaccggagct gattggcggc ataaaagttg aagtgggtga tcaggttttg gatttgtctg
    15001 tacaaggcaa attgagtgcc ttgtacacga ctatgacgaa ttaggagagt tttcatgcag
    15061 cttaatcctg ctgaaattag cgatttgatt aaagccaaga tcgaaaatct gtctgtaaat
    15121 gccgaagtgc gtacccgtgg tacggtgatt tcggtaactg acggtatcgt tcgcatccat
    15181 ggtttgtcag atgcaatgca aggtgagatg ctcgaattcc cgggtaacac tttcggcttg
    15241 gcgatgaacc tggagcgcga ctccgtcggc gccgtagtgt tgggcgagta cgaacatatt
    15301 aaagaaggcg acacggttac ctgtaccggt cgtatcttgg aagtgccggt cggacgcgaa
    15361 ctggtcggac gcgtcgttga tgcattgggt cggcctatcg acggcaaagg cccgattaat
    15421 acaactttga ccgcccctat cgaaaaaatc gcaccgggcg tgattgcgcg caaatcggtt
    15481 gaccagccga tgcaaaccgg tctgaaggcg attgactcta tggttcctgt cggtcgcggt
    15541 cagcgtgagt tgattattgg cgaccgtcag acaggtaaaa cggccgtagc attggatgcc
    15601 atcgtcaacc aaaaaggtac gggtgttatc tgtatctatg tcgctatcgg tcaaaaagca
    15661 tcttctattg ccaacgtggt ccgcaaattg gaagagcatg gcgcgatgga gcataccatc
    15721 gtggttgctg caactgcatc tgaagcggcg gcattgcaat atatcgcacc ttactccggt
    15781 tgtacgatgg gtgaattctt ccgcgatcgt ggcgaagatg ccttgattgt ttatgacgat
    15841 ttgtccaaac aggctgtggc ttaccgtcaa atttccctgc ttttgcgccg tccgcccggc
    15901 cgcgaagcct atcccggcga tgtgttctac ctgcactccc gtctgttgga acgtgcggca
    15961 cgtgtcaatg aacacgaagt ggagaaattg accaacggcg aagtaaaagg caaaaccggt
    16021 tctctgaccg cgttgccgat tatcgaaacc caagcgggcg acgtatctgc tttcgttccg
    16081 actaacgtca tttcgattac cgacggtcag attttcttgg aaaccgacct cttcaacgcc
    16141 ggtatccgtc ctgcaatcaa tgccggtatt tccgtatccc gcgtaggcgg tgctgcacaa
    16201 accaaagtga ttaaaaagct gggtggcggt atccgtttgg cgttggcgca atatcgtgaa
    16261 ttggcggctt tctcgcaatt tgcatccgat ttggatgaag ctacgcgcaa acaattggag
    16321 catggcgaag tcgtaaccga actgatgaaa cagaaacagt tcagtacgtt gaatacggct
    16381 gaaatggctt tgaccctttg ggcaatcaac aacggttcgt attctgatgt tccggttgcc
    16441 aaagctctgg cttttgaatc tgaatttttg agctttgtcc gtacccaaca tccggaagtt
    16501 ttggaagccg tcaatgcttc aggtgcaatg tccgacgaga gcgagaaaac gcttgaagca
    16561 gccatgaaat ccttcaaatc ttcttacgcc tatcaggcat aaggcttgaa gtgaaaggag
    16621 tctgaaatgg cagtaggaaa agagattctc accaaaatcc gcagtgttca gaatacccaa
    16681 aagatcacta aagcgatgca gatggtgtca acctctaaaa tgcggaagac tcaggaacgg
    16741 atgcgtttgg cgcgtccgta tgccgaaaaa gtgcgtatgg tgatgagcca tcttgcgcaa
    16801 accaataccg atcatggtat tccgttactg gaatctcatc gggaaatcag acgtgtcggt
    16861 tttattttga ttacgtctga taagggtttg tgtggcggtt tgaacgccaa cgtgctgaaa
    16921 aagtttttgg cacaagttca agagtatcgg aatcaaggta ttgaagagga aatcgtatgc
    16981 cttggcagta aaggtctgat ggcgtgtcag agcattggtc tgaatgtggt tgccagtgcc
    17041 gtaaatttgg gcgatacccc aaaaatggaa atgctgctcg gacctttgac agaactcttc
    17101 caacggtatg agaaacatga aattgacaga atccatctgg tgtattcggg ttttgtcaat
    17161 accatgcgtc aagaaccgag aatggaagta ttgctgccta tcggtgagaa cgtgattggc
    17221 gattcagctc ccaaatcacc gttcagctgg gaataccgct acgaaccgac tgcacttgca
    17281 gtgttggaat atttggttcg ccgctattta gagtctgtgg tttatcaggc gttgagcgac
    17341 aatatggcat ccgaacaggc agcccgcatg gttgccatga aagctgcaac agacaatgca
    17401 ggcaatgcca tcaaagagtt gcgtttggta tataacaaat cgcgtcaagc tgcgattacc
    17461 acggaattgt cagaaattgt agcaggtgcg gcggctgtct gatgccgtct gaatcctgta
    17521 cggaaattta ggatacgata atgagccaag gcaaaatcgt acaaattatc ggtgcggttg
    17581 ttgacgtgga atttccacgc gacatgattc cgcgcgttta cgacgctttg aaattagacg
    17641 aaaacggtct gactttggaa gtccaacagc ttttgggcga cggcgtagtc cgtaccattg
    17701 cgatgggcag ctcggacggt ttgaaacgcg gcatgactgt gagcaatacc ggtgcgccca
    17761 ttactgtgcc ggtaggtaaa ggtacgttgg gacgcattgt cgatgtattg ggaacgcctg
    17821 ttgacgaggc aggtccgatc gataccgaca agagtcgtgc catccaccaa gccgctccta
    17881 agtttgacga actgtcttcc acaaccgaat tgctcgaaac gggcattaaa gtgatcgact
    17941 tgctgtgtcc gtttgctaag ggcggtaaag taggtctgtt cggtggtgcc ggtgtgggca
    18001 aaaccgtgaa catgatggaa ttgatcaaca acatcgccaa agcgcacagc ggtctgtccg
    18061 tgttcgcagg tgtgggtgag cgtacccgcg aaggtaacga cttctaccac gagatgaaag
    18121 attccaacgt attggataaa gtggcaatgg tttacggtca gatgaacgaa cctccgggca
    18181 accgtttgcg cgtcgcattg accggtttga ccatggcgga atacttccgt gacgaaaaag
    18241 acgaaaacgg caaaggccgc gacgtattgt tcttcgtgga caacatctac cgttacaccc
    18301 tggccggtac cgaagtatcc gcattgttgg gccgtatgcc gtctgcagtg ggctaccaac
    18361 cgacattggc agaagaaatg ggtcgtttgc aagagcgtat tacctctacc caaaccggtt
    18421 ccattacttc catccaagcc gtatatgtac ctgcggacga cttgaccgac ccgtctccgg
    18481 caacaacgtt cgcccacttg gacgcaaccg tcgtattgag ccgcgatatt gcctctttgg
    18541 gtatttaccc ggcagtcgat ccgcttgatt ctacttcgcg ccaactggat ccgatggtat
    18601 tgggtcaaga gcactacgac gtagcgcgcg gcgtacagtc caccctgcag aaatacaaag
    18661 aattgcgcga catcatcgcc atcttgggta tggacgaatt gtctgatgaa gacaaactga
    18721 ccgtaatgcg tgcgcgtaaa atccaacgct tcctgtccca accgttccac gttgccgaag
    18781 tgtttacagg ttctccgggt aaatatgtcg ccctgcgcga taccattgcc ggcttcaaag
    18841 ccatcttgaa cggcgaatac gatcatctgc ccgagcaggc attctatatg gtcggcagca
    18901 ttgaagaagc agtagagaaa gcgaaaacct taaactaagg aggtcggcat gagcatcatg
    18961 caagttgagg tggtaagtag cgagcagaaa atctattcag gcgaggcaac atttatcgtt
    19021 gttccgactg tacagggcga actcggtatt tatccgcgac acgagccgat tatgagtttg
    19081 gtgcggccgg gggctttgcg tttgaccgtt ccgggcgagg ataaagaggt tttggttgct
    19141 gtttccggcg gtgttttgga agtacagcct gataaagtaa ctgtcttggc ggatgttgcc
    19201 gtccgcagtg cagagatgga tcaggcacgt gcggaagagg cgaaaaaagc cgcagaagcg
    19261 ggcatttccc aagctaaaga cgataaggct ttggcggagg cgcataaagc attggctgct
    19321 gcgattgcac agctcaaaac tttggactat atccgttcgc acaagaaata aacttgtttg
    19381 gtttggaaaa gcacgaccgt ttggtcgtgc tttttatttt tccaagtttt gaaa
//
LOCUS       DAWFJW010000030        19337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFJW010000030 DAWFJW010000000
VERSION     DAWFJW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.030"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(31..1557)
                     /locus_tag="WGR19_000858"
     CDS             complement(31..1557)
                     /locus_tag="WGR19_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0798410.1"
                     /translation="MKTRSLLPLLFLLLCSCAAWLPPLDERTESRHFDTASPVRLDKV
                     LHIRHFPSDSRLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAERLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            complement(1667..3073)
                     /locus_tag="WGR19_000859"
     CDS             complement(1667..3073)
                     /locus_tag="WGR19_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0798411.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSAAACLLLLAA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FNRLHVSDGTNLTRNTPS"
     gene            3357..4136
                     /locus_tag="WGR19_000860"
     CDS             3357..4136
                     /locus_tag="WGR19_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0798412.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            4133..4834
                     /locus_tag="WGR19_000861"
     CDS             4133..4834
                     /locus_tag="WGR19_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0798413.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNISAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            4831..6285
                     /locus_tag="WGR19_000862"
     CDS             4831..6285
                     /locus_tag="WGR19_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0798414.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6468..6953)
                     /gene="purE"
                     /locus_tag="WGR19_000863"
     CDS             complement(6468..6953)
                     /gene="purE"
                     /locus_tag="WGR19_000863"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0798415.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(7001..7717)
                     /locus_tag="WGR19_000864"
     CDS             complement(7001..7717)
                     /locus_tag="WGR19_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0798416.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(7717..8385)
                     /locus_tag="WGR19_000865"
     CDS             complement(7717..8385)
                     /locus_tag="WGR19_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0798417.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVSFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(8396..8581)
                     /locus_tag="WGR19_000866"
     CDS             complement(8396..8581)
                     /locus_tag="WGR19_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0798418.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            8724..10700
                     /gene="mutL"
                     /locus_tag="WGR19_000867"
     CDS             8724..10700
                     /gene="mutL"
                     /locus_tag="WGR19_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0798419.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLQERIAAIVGDDFQT
                     ASLEIDSSNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHDITGVTPAPMPSENDGENLFDRASNYPTGNKPDTRNAFGASGKTAPMP
                     YQAAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRLEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPATFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSVRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            10788..12917
                     /gene="dnaX"
                     /locus_tag="WGR19_000868"
     CDS             10788..12917
                     /gene="dnaX"
                     /locus_tag="WGR19_000868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0798420.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPITVLSRCLQFVLRNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANTVIENTELQSPSAQTAEKETAAK
                     KPQPRPEAETAQTSVQTASAAAMPSESKTAEPVTNQENNDVPPWEDAPDEAQTAACTA
                     QASAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    11977..12016
                     /gene="dnaX"
                     /locus_tag="WGR19_000868"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            12997..13332
                     /locus_tag="WGR19_000869"
     CDS             12997..13332
                     /locus_tag="WGR19_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0798421.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(14061..15107)
                     /gene="recA"
                     /locus_tag="WGR19_000870"
     CDS             complement(14061..15107)
                     /gene="recA"
                     /locus_tag="WGR19_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0798422.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGKLDETDGERLEE"
     gene            15361..16125
                     /locus_tag="WGR19_000871"
     CDS             15361..16125
                     /locus_tag="WGR19_000871"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0798423.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAVLSAVLDQIKNLPFD
                     IAEFRADFLECAGSIGEVLHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKQLVAKPIVTMSMGQKGAVSRLAGQT
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLENGAD"
     gene            16145..18160
                     /gene="rep"
                     /locus_tag="WGR19_000872"
     CDS             16145..18160
                     /gene="rep"
                     /locus_tag="WGR19_000872"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0798424.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKINHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYQLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETSEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(18228..19286)
                     /gene="dinB"
                     /locus_tag="WGR19_000873"
     CDS             complement(18228..19286)
                     /gene="dinB"
                     /locus_tag="WGR19_000873"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0798425.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         4700 a         5408 c         5249 g         3980 t
ORIGIN      
        1 cgtactggtt tttgttaatc cgctatattt ctataataaa ctttctatgg gcagcaggga
       61 taggattttt gcggcgatgc gtttccaaag tttggcttcg ggttcgttcg ggtaggtttt
      121 tcgggtggcg ggatcatgcc attgcaggcg gttgtgcctg tcgagggtaa cgcggtaggc
      181 gtattcgggt gtggtatcgg caagggtgcg ctccatctgt tctgcgattt tggggctttc
      241 gataacaacg cccatttcgg tgttgagacg cgcggaacgg gggtcgaggt tgaacgaacc
      301 gatgaagatg cgtttgccgt ccacaatgaa ggttttggcg tgcaggctag ttacggagct
      361 gccggtcagg cctttgtctt ttgtggcggg gacggcatgg ttgggttgca gctcgtagag
      421 tttgatgccg gctttgagca gcggttttcg gtatttgaca tagccggaat ggacggcggc
      481 aacgtcggtc gcctgtagcg agttggtcag aacggtaacg tctatgccgt cctgcaccag
      541 ttttgccagt gcgtctgtgc cggattttgt ggggacgaaa tagggtgaaa ccagatagac
      601 gcttttttcg ggctgtttga gcgcgtcttg cagccgctcg gcaatcggcg gtttgcggcg
      661 gtcgcggtcg agtccttttg cagggtcgtc gctgatgagg cgggtttgga cgctctgcca
      721 gtcgatgcgt cccgcctgta ttttttggta gaggggcgac tgttcgacgg tttcgcggta
      781 gcgcaggagc gcgtgtctgg acgtttcgtc gttgtatccg agtgcttgaa gacccttgcc
      841 gatgttgccg ctgcggatga tgcgcgtggc gttgtgggcg gaatggcttg cccagtagcg
      901 gtcgaagtcg tgcgatactt cgccgacgac gctgccggtg gcgaggatgt ccaggtcggc
      961 gaaaacggtg tcctcaccga ctttgaagta ttcgtcgccg atattgcgtc cgccgagtat
     1021 ggtggcgcgg ttgtcggcgg taaaggattt gttgtgcatg cggcggttga ggcgggggaa
     1081 gtcggtcagg tagccgagtg cgcgccattt tcgtaagacg aaggggttga acaggcgcac
     1141 ttcgatattg ggatggctgt cgagggcaag caggaggtcg tccaatccgc gcgtgttgtt
     1201 gtcgtccaac agcaggcgta cgcgcacacc gcgttctgcg gcaaggtaca cgaggttgaa
     1261 cagcagtcgg ccggaaatgt cgttgcgcca gatgtagtat tgcaaatcga ggctgtgttc
     1321 ggcagattca ataagggcag cgcgggcggc aaaggcttcg tgggggtcgt cgagcaggta
     1381 aatgtcggac aggcggctgt cggacgggaa atggcggatg tgcaggactt tatccaaacg
     1441 gacgggggaa gcagtgtcaa aatggcggct ttccgtgcgt tcgtccaagg gcggcagcca
     1501 tgcggcgcac gagcagagca ggaggaacaa caggggaagg aggctgcgtg ttttcatagg
     1561 aaatcggttc agacggcatt gcctgtgttt tggggttggt gcgcatggaa gtgcggcatc
     1621 ataatccaaa cgttgaaacg ggtaaaagtt ttgcgtgcgg accgcttcag gacggtgtat
     1681 tccgtgtcag gttggtgccg tctgaaacgt gcagccgatt gaataccagc gatgaggcaa
     1741 gggtgatgcc gccgatgctg agcagggtca tacggaaggc ggaatgcaga cctgaagaag
     1801 ccggtatcag aaatgtccag tttttaagga ttaatgcgcc gacaacaatg cccatgctga
     1861 tggcaagctg ttggttgacc gccatcaggc tgttgccgct gccggtctgt tgcgggcgca
     1921 aatcggcgag ggtcagtgta ttcatggcag aaaactgcag ggagttgcac gcgccgatcg
     1981 ccagcgagag gaaaatccaa atccacagcg acgagtttcc gtcaggcagg gcgagcagca
     2041 tgatgaaggc ggcaagcagc ttggtgttcc aaagcagtac cgtgcggtag ccgaaacgtt
     2101 tcatgagcgg tgcaatcagc ggtttgacca gcagcgaaga cagggcgacg ggggcgacca
     2161 gccaacccga caggcttgcg ccgaagccga aagcgacttg aaacatcagg ggcatcagaa
     2221 aaggaatcga gctgatgccg agacggctga acagattgcc tgccagtccc agacggaaag
     2281 tgcgtatcag aaacaggtcg gcggaataaa tcggtttgga cgtggttttc atatgtcgga
     2341 aataacggcg tgcaaacagc agtccgccgc acagcggcaa cagtgcaaaa tacggaggca
     2401 gcgcgtgcga caggctttct gcagcaagca gtagcaggca ggcggcagcg gaaaaggtca
     2461 gatagccttt aaaatccaga gcggtatcgt tgcctttgac atcgggcatg atgttgcgtc
     2521 ccaatataaa ccccagcaga ccgatgggca ggttgagcag gaaaatccag tgccacgaag
     2581 cgtattcgac cagataaccg cccgccaaag gccctaaaac cggcccgatt aatgcgggca
     2641 taaccgcata attgatggcg ttgagcagct tggacttgtc gtacatacgc aagatggtca
     2701 gacgcggtat cggaaccagc atcgaaccgc cgatgccctg aacgacacgg gaaagcgtca
     2761 attcaaacag cgaacccgat gcggcgcaca atgccgatcc gagcataaaa acggcaatcg
     2821 aaccgaaaaa gacttttttc gttccgaacc tgtccgccaa ataaccgctc aaaggaatca
     2881 gcagggcaac cgtcagcgta taggcaacga ctgccagttg catatccaga ggcgattcgt
     2941 ccaggtcggt ggcaatttca ggcagtgcgg tatttaaaat ggtcgcatcc aacatctgca
     3001 taaaaatggc aattgccagc agaagcggca gccaagggga tggtgcgcgg gcggataggg
     3061 tgtttttttc catagggcga ttgtacccca tccttgtgcc gttattgttt tcagacgctg
     3121 tctgaatgcc gtcagagtcg gcatcttgaa tgttcacaag caaacgaacc ggtattgcat
     3181 tgtaatgata attattatcg aaaaacatca gattaaggta cagtaagcgt tatggggtag
     3241 tttgtaagaa aaaccggatt attttttaaa attagacttg acccgcaaca gtcaattact
     3301 taaagtaaac gcttaccttt ctacagaaaa aaacgggttt cccgttatca aaaaacatga
     3361 gcgcaaccat tcccccaaaa atcatccgat acgacagcaa tccgaccgat gtctattttt
     3421 tcggcacttg cgtccttgat ctttttatgc ccgaagcagg catggatgcc attaccctaa
     3481 tcgagcaaca gggcatacgc gtccatttcc cgatggcgca aagctgctgc ggccagcctg
     3541 cctattcatc cggccatccg accgaagcct tcgatgtcgc caaagcacaa ctcgaccttt
     3601 tccctgaaaa ctggccgatc gtcgtgccgt ccggctcgtg cggcggcatg atgaaacacc
     3661 actggccgac gctgtttaaa ggcagcgagt acgaggaaag ggctgtggat tgcgccggcc
     3721 gcatcatcga gtttacccat ttcctgcttg ccatcggttt caaacccgaa gacaagggcg
     3781 aaccgctcaa agtcgccgtt cacacttcct gcgccgcccg ccgcgaaatg aatgtccatc
     3841 tttcaggctg gcaactgatt gacggtatgg aaaacgtcga acgcatcgtc cacgaccacg
     3901 aaagcgaatg ttgcggcttc ggcggcacat tctccgtcaa acaagccgat atttccggcg
     3961 caatggtaac agacaaagtc gccgcgctga aagaaaccgg cgcaaccgaa atcatcagcg
     4021 cggactgcgg ctgtatgatg aacatcggcg gcaaaatcgc caaggacgag ccggatatgc
     4081 cgcgtccgaa acatatcgca tccttcttgt tggaacgcac cggaggcaaa gcatgagcgc
     4141 gcgtgaaaat attttggcaa aactgaaaaa agccgatgcc ttgccgatgg aagagcctgc
     4201 ggttttcgat tattaccgcg aaaagggcgt ttcttgggac agcgaagccg agcgtctgaa
     4261 acattgggct gccgcaatgc gcgcggtcaa aaccgaaatt tattgggtga cgaaaagcaa
     4321 ttggatgcag gttttccgcg aagcggcaga aggcaagggt ttgaaaaaca tcctgctgcc
     4381 cttggcgacc gaacacggac aaattgcccg tgccgcattg gcggacagca atatcgaacc
     4441 gattgccttc gagcgcgaaa tcgatacttg gaaaaccgag tttttcacga acatcagtgc
     4501 gggcttcagc ggcgcgcaat gcggcatcgc ccgcaccggc acgctgatgc tgttttccag
     4561 ccccgaagaa ccgcgtactt tgagtctcgt tccgcccgtg catttctgcc tgttcgatac
     4621 gtccaagatg tacaacgagt ttcataatgc cgtcgaaggc gaaaaactgg tggaaaacgg
     4681 tatgccgacc aatgtattcc tgatttccgg cccgtccaaa accgcagaca tccaactgac
     4741 gcttgcttac ggcgcgcacg gcccgcgcga tttggtcatc ctcgccatcc tgcccgacca
     4801 catttcccct gccgatttgg aggaaaacgc atgactacgc aaaccatcaa gttccacatg
     4861 aagccggaaa ctttcaagca aaacgccgca atttcccttc aagacaagcc tttgcgcaaa
     4921 agcctgcgta ccgcgatgga tatgctgatg accaaacgca aagccgtttt gaccgacgaa
     4981 gaagagctgc aaagcctgcg cgatttgtgc gaacacatcc gccagcgttc cctgtccaaa
     5041 ttgcccgcgc tgttggagca gttggaagaa aacctgacca agttgggcgt gaaagtacac
     5101 tgggcggaaa cccctgccga agcctgccaa attatccacg acatcatcac agccaaaaac
     5161 ggcaagctga tggtcaaagg taaatccatg gtcagcgagg aaatcgagct gaaccattat
     5221 cttgaagcaa aaggcattaa agcggtagaa agcgacttgg gcgagttcat cgtccaaatg
     5281 gcaggcgaaa aaccgaccca tattgtgatg cctgctatcc acaaaaccaa agaacaggtt
     5341 agcgaactgt tccaccaaaa cctcggtacg ccgctgacag acgatgtaga ccaactgacc
     5401 ggcttcgccc gtaaagcact gcgcgatatt tacagcactg ccgatgtcgg tttgagtggc
     5461 gtaaacttcg ccgttgctga aacaggtacg ctgtgtctgg tggaaaacga aggcaacggt
     5521 cgcttgagta ccaccgtacc gcccgtgcat atcgctatta ccggcattga aaaagtggtg
     5581 gcgaaattgt ccgacatccc acccttgtac agcctgctgc cgcgttctgc cattggtcag
     5641 aacattacca cttatttcaa tatgattacc ggcccgcgcc gtagcgaaga gctggacggt
     5701 cctcaagaaa tgcacttggt cctgcttgac aacggtcgca gccaagccta tgccgaagac
     5761 caaatgcgcc gcaccttgca atgtatccgt tgcggcgcgt gtatgaacca ttgcccggtt
     5821 tatacccgta tcggtggcgc ggcatatggc acaacctatc ccggtccgat tggcgagatt
     5881 atttccccgc acctgttagg cttggatgcc acccgcgacc tgccgaccgc ctgtaccatg
     5941 tgcggtgcgt gtgtggaagt ctgtccggta cgcatcccga ttaccgaaca aatgcagcgt
     6001 ttgcgcgttg aagcgcaacg ttcgccgacc gaaaccgtgc cgcaccccat ccgagggcaa
     6061 ggcgcatcgc ataccttcgg cgaacaaatg gcgtggcgca cattcaacgg tattttcagc
     6121 ggcagcaaaa cctaccgcgc cttcggttgg gcagccacca agttccgcaa cctgaccccg
     6181 cgcaaacagt tgggttggac gcaaaaccgc gtgccgatga aaccggcgaa gaaaaccctg
     6241 cacgaactaa tggcagaaaa aatgcgccaa aaagaacagg cataaaaagt tgttcgcaaa
     6301 aatgccgtct gaaacccgaa acagggcttc agacggcatt tgtatagtgg attaacaaaa
     6361 atcaggacaa ggcgacgaaa ccgaagacag tacaaatagt acggcaaggc gaggtaacgc
     6421 cgtactggtt taaatttaat ccactatata ttcgcggacg gcgggtttta aatctgttcc
     6481 aattccatat tcaaaacagt ctgttcttgt ttggctcgga agtctgccag tttttgcgcc
     6541 agttcggggt tttcgttggc gagcatggaa acggcgaaca atgcggcatt tgccgcacct
     6601 gcctcgccga tggcgaatgt ggcgacgggt acgcctttgg gcatttgtac aatcgataaa
     6661 agcgaatctt cgccgcgcag gtatttgctg gggacgggta cgcccaaaac ggggacggtg
     6721 gtcttggcgg caaccatacc gggtaaatgc gccgcgccgc ccgcacccgc gatgatggct
     6781 ttgatgccgc gcgcccgtgc ggtttcggcg tattggaaca tcaaatccgg ggtgcggtgt
     6841 gcggaaacaa cgcgcgcctc atattctacg ccgaactctt caagaaactg cgctgcctgc
     6901 cgcataacgg gccaatcgct gttgctgccc atgatgatgc cgatttgtat cataaatcct
     6961 ccttggtgcg gatggggtaa aaagcggaaa aatggaaaaa ctatcgtttg cgcacggctg
     7021 cggcggcgcg ttttgccgcc gggctgctcg ggtaagcctg tatcaggctg cgccaagtcg
     7081 cccttgcaat gtctttctgc tgcaacctgt attggcattc gccgattttg aatatggctt
     7141 cgggcgcggt tgggctgtct ttgaaacggt tggcgtaacg ccctccgatt tcgatgacgg
     7201 attcgcagtt gcccatacgc gccctgcttt gcagcaacag atacatactg cgttgcgcga
     7261 tgctgccgcc gtcgcctccg tccgcgcctt tcaacaggga ggcagcggca gaaaacctgc
     7321 cgcttttata gtgtttgagt gcctgattgt agaggttttg tgcggtttcg acggtatgtg
     7381 cggatgcgct gccgccttcg gtattgaggt aatgctcttt caacttgcgg tcgtcgagtt
     7441 tttggacgta tgccctgctg gaagggtgtg ttttcgcatg ctccagtgct ttgactttgc
     7501 cgtttaaggt ttccacttcg ttcgacaggc ggacgagtgt gccttccaga taatccaaac
     7561 ggtcttgcag agtgggaacg ggatagggaa tagcgtccga actacctgcc tctgcctgcg
     7621 gttcgggccg catatcctga atattgcggg aaacagggga agaacaggcg gcggatacgg
     7681 acagccaaat gataaaaagc ggtaatttgg tcttcattat tttttcagaa gcagggtcaa
     7741 gccgtcgccg acgggcaggg tgatggggac aatccgcgta tcgttcggca ggttttgatt
     7801 gaaatctttg aggatgccga cgctgggcgg cgcatcggaa gccgcttcgc gcatcaccct
     7861 tccgttcagc aaaatattgt cgatggcgat gatgccgcct tgacggacga gtttgaggca
     7921 acgctcgaaa tatcgcggcg tgggcggttt gtctgcgtct atcagtgcca aatcgtagct
     7981 tccggcttca ccctgtgcaa tcaaatcatc caatgtcagc aatgcgggtt gcaggtgcag
     8041 gctgatttta tgtgccacac cggcctcgtt ccaaacctga cgcgccgtat cggtaaagct
     8101 tacattgatg tcgcaggcgg taatccgccc gtgttcgggc aacgccaatg caagcgcggt
     8161 gctgctgtat ccggtaaata cgccgatttc cagatatttt tccgcacgga tcagctttgc
     8221 cagccaaacc aaaaccgccg cctgttcgcg cgcaatcgcc attttgccca tacggtgatg
     8281 cccggtcttc tcacgcagcc gcgtcaaaac gggatgttca ggttcgccga tggcgttcaa
     8341 atagttttgc aggtccggtg caacattgga cagatgggtc gtcatttcgg cgggttcagt
     8401 cttggtaata ggtataaggt tttttcgcta cttttgccgc ctcgaagttt tcctgttctt
     8461 cgggattgag ttcgacatcc cacaaaagcc cccggttttc caaacgctgc tgttccaact
     8521 caggtttttc ttcaatcagg cggttgagga attgtgtggc ttcggattgg tagtgataca
     8581 tctttgcgct ccaattttac gggatacggc gtgattatac tggtattttc caaacgggat
     8641 aaacggcttt tatcaagaat acaggcagaa agataagggg ttttattata gaataaagcg
     8701 tttttgcaac ggaagcctac cttatgccac gcattgccgc cctgcccgac catctcgtca
     8761 accaaatcgc cgccggcgaa gtagtcgaac gccctgccaa cgccttgaaa gaaatcgttg
     8821 aaaacagtat cgatgcaggc gcaacggcga ttgatgtcga gctggacggc ggcggcatcc
     8881 gcctgattcg cgtcagcgac aacggcagcg gtatccatcc ggacgacatc gaactcgcgc
     8941 tccaccgcca cgccaccagc aaaatcaaaa ccttaaatga cttggaacac gtcgccagca
     9001 tgggctttcg cggcgaaggc ttggcaagca tcgcctccgt cagccgcctg accctgacca
     9061 gccgtcaaga agacagttcg cacgcaaccc aagtcaaagc cgaagacggc aaactcagca
     9121 gccccaccgc cgccgcccac cccgtcggca ccaccatcga agctgccgaa ctcttcttca
     9181 acacccccgc gcggcgcaag ttcctcaagt ctgaagccac cgaatacgcc cactgcgcca
     9241 ctatgctcga acgcctcgca ctggcgcatc cgcacattgc cttctcgctc aaacgcgacg
     9301 gcaaacaagt attcaaactt cctgcccaaa gcctgcagga acgcatagcc gccattgtcg
     9361 gcgatgattt tcagacggcc tcattggaaa ttgacagcag caatggtgca ttgaggcttt
     9421 atggcgcgat tgccaagccg acttttgcca aaggcaaaac cgacaaacaa tactgcttcg
     9481 tcaaccatcg cttcgtgcgc gacaaagtca tgctccatgc cgtcaagcag gcataccgcg
     9541 acgttttgca caacgccctc acccccgcct tcgtcctctt cctcgacctg ccgcccgaag
     9601 ccgtggatgt caacgtccac ccgaccaaaa ccgaaatccg cttccgcgac agccggcagg
     9661 tgcaccaact cgtgttccat acgctcaaca aagcccttgc cgacacgcgc gccgacctga
     9721 ccgaaagcgt cggcaacgcg ggcgaagtgc tgcatgacat taccggcgtt acgcctgcac
     9781 caatgccgtc tgaaaacgac ggcgaaaatc tgtttgatcg cgcatccaac tacccgacag
     9841 gcaacaaacc cgatacgcgc aatgcctttg gtgcatcagg caaaaccgcg cctatgcctt
     9901 accaagccgc atatgcgccg caacaacgca gcctgtccct gcgcgaaagc cgcgcggcaa
     9961 tgaacaccta tgccgaactc tacaaaaaaa ccgacgacat cgaccttgag ttgagccgat
    10021 tagagcaggc acgtttcggc aatatgccgt ctgaaacgcc tgctccccaa acagatacgc
    10081 cgctttcaga cggcatccca tcccaatccg aactaccgcc gctcggtttt gccattgccc
    10141 aattactagg catctacatt cttgcccaag ccgaagacag cctgctgctc atcgatatgc
    10201 acgctgccgc cgaacgcgtc aactacgaaa aaatgaaacg ccaacgtcag gaaaacggca
    10261 ggctgcaaag ccaacgcctg cttattcccg caaccttcgc cgcgtcccac gaagaatgcg
    10321 ccgcccttgc cgaccacgcc gaaacactgg caggcttcgg gctggaactg tccgatatgg
    10381 gcggcaacac cctcgccgtc cgcgccgcac ccgcaatgtt gggcaaagcc gatgtcgtct
    10441 cgctcgccaa agacgtatta aacgaacttg cccaagtcgg cagcagccaa accatcgaag
    10501 aacacgaaaa ccgcatcctc gccaccatgt cctgccacgg ctcagtccgc gccggccgcc
    10561 ggcttaccct gccggaaatg aatgcgctgc tgcgcgatat ggaaaacacg ccgcgcagca
    10621 accagtgcaa ccacggcaga ccgacttggg tcaaactgac tttgaaagaa ttggatgcct
    10681 tgtttttacg tggacagtga accccttcgt gagccggttc gaaaaatgct agaatacgcg
    10741 cccggatacg tcccgaacgg acaaaacgct taatcaaaga gaaaaatatg gcttatcaag
    10801 ttcttgcacg aaaatggcgt ccgaaaacct ttgccgactt agtcggtcag gaacacgtcg
    10861 tcaaagccct gcaaaacgcg ctggacgaag gcaggctgca ccacgcctac ctgctgaccg
    10921 gtacgcgcgg cgtgggcaaa accaccatcg cccgcatcct tgccaaaagc ctcaactgcg
    10981 aaaacgcgca acacggcgaa ccttgcggcg tgtgccaaag ctgcacccaa atcgatgccg
    11041 gacgctatgt cgacctgctc gaaatcgacg ccgcctccaa cacaggcatc gacaacatcc
    11101 gcgaagtctt ggaaaacgcc caatacgcgc cgaccgccgg caaatacaaa gtctatatca
    11161 tcgacgaagt gcatatgctc tccaaaagcg cgttcaacgc tatgctcaaa acgctggaag
    11221 agccgcccga acacgtcaaa ttcatccttg ccacgaccga tccgcacaaa gttcccatta
    11281 ccgtcttgag ccgctgcctg caattcgtct tgcgcaatat gacttcgcag caggttgccg
    11341 accacctcgc ccacgtcctc gacagcgaaa aaatcgccta cgaacccccc gccctgcaac
    11401 ttttgggacg tgccgccgcc ggatcgatgc gcgatgcctt gagcctgctc gaccaagcca
    11461 tcgccctagg ttcgggcaaa gttgccgaaa acgatgtccg ccaaatgatt ggcgcggttg
    11521 acaaacaata cctttacgaa ctgctgacag gcatcatcaa ccaagacggc gcagccctga
    11581 ccgccaaagc gcaggaaatg gcggcgtgtg ccgtcggctt tgacaacgcc ttgggcgaac
    11641 tggccatact gctgcaacaa ctcgccctga tacaggcagt gccgaatgcc ttggcgcacg
    11701 acgaccccga ttccgatatt ttgcaccgcc tcgcccaaac cataagcggc gaacaaatcc
    11761 agctttacta ccaaatcgcc gtccacggca aacgcgacct gagcctcgcc cccgacgaat
    11821 acgccggctt tatgatgacc ctgctgcgta tgctggcgtt cgcacccttg gcggcagctt
    11881 cgtgtgatgc aaacaccgtg attgaaaata ccgaacttca atccccatcg gcacaaaccg
    11941 ccgaaaagga aaccgccgca aaaaagcccc aaccgcgccc tgaagcagaa accgcccaaa
    12001 catccgttca gacggcatcc gcagcggcaa tgccgtctga aagcaaaacc gccgaacccg
    12061 ttaccaatca agaaaacaac gatgttccgc cttgggaaga cgcgccggac gaagcccaaa
    12121 ccgcagcctg cacggcgcaa gcatcggcaa aaagcattca gacggcatcc gaagccgaaa
    12181 cgccgcccga aaaccaagtt tccaagaaca aggcagccga caacgtaacc gatgccccct
    12241 tgtccgaagt gccgtctgaa aaccccattc aggcaacacc gaatgatgaa gccgttgaaa
    12301 cggaaacatt tgcacacgaa gcccctgcag aacctttcta cggttacggc tttccggata
    12361 atgactgccc cgtagaagac ggcgcagaaa tcccgccgcc cgattgggaa cacgccgccc
    12421 ctgccgatac cgcaggcgga ggcgcggacg aagaagcaga agcaggcggc atcggcggaa
    12481 acaacacgcc gtccgccccg ccgcccgaat tttccaccga aaactgggca gccatcgtcc
    12541 ggcatttcgc ccgcaaactc ggcgcggcgc aaatgccggc gcaacactcc gcgtggacgg
    12601 aataccatcc cgacaccggc ctgatggttt tggcgatgac cgccgaagcc cgcgccaccg
    12661 ccgacaaaaa acgtctcgac aaaatccgcg acacccttgc ccaagcctac ggactgcaac
    12721 tcaccctgca aacccaagac tggcgcgacg aagccggcag ggaaaccccc gcgatgcagg
    12781 acaagcgcgt ccaagccgaa gacaggcaaa aagcacaagc attgctcgaa gccgaccccg
    12841 ccgcacaaaa aatcctccaa gcattcggcg cgcaatggca gcccgaatca ctggaattgg
    12901 cagcaaaccg gccataaaca gatataatgc cgcccgaacc cttcggacgg cattgccgtt
    12961 tccctcattc aatcaaaaca gacaggagta ttcagtatgt tcggaaaagc cggattaggc
    13021 ggcctgatga aacaggcgca gcaaatgcag gaaaatatga aaaaagcgca agccaaactt
    13081 gccgaaaccg aaatcgaagg cgaagcaggc aacggcctgg tcaaaatcac aatgacctgc
    13141 gcgcacgaag tacgcaaaat cgacatcagc cccgatttga ttcaagaagc cgccgacgac
    13201 aaagaaatgc tcgaagacct catcctcgcc gccctcaaat ccgcccgtga caaagccgaa
    13261 gaaaccgcaa acaaaacaat gggcgcattc acgcaaggtc taccccccgg agtgggcgac
    13321 ttcttccgct gatccccgac cgtcattccc acgcaggcgg gaatctagaa cgtagaatct
    13381 aagaaaccgt tttactcgat aaatttccgt gccgaggggt ctggattccc gccttcgcgg
    13441 gaatgacggc atcagtttgc aggattcggc ggaaacggta aaaacggcag aatcgatggg
    13501 aatcggcagg ctgaagccca ccctaccatt atttacacat ccgtaccgct taaatgccgt
    13561 ctgaaacttc gtcattcccg tgaaaacggg aatccagccc cgtgggagca gaaacttaca
    13621 ccccgtcatt cccgcgaacg cgggaatcca gtaaccgaaa aaccacagga atctatcgga
    13681 aaaacagaaa cccccgaccg tcattcccgc gaacgcggga atctagaacg tagaatctga
    13741 gaaaccgttt tactcgataa atttccatgc caacaggtct ggattcccgc cttcgcggga
    13801 atgacggcat attttttgca tttgatataa agggtcgttt gaattttgtt cagcaagtgc
    13861 aaagtgttgc acataaaagg gcgtaggctt catccgtaaa gtcggcgcag gatagaggca
    13921 aagcgggcgt aggtcgggct gtagcaactg tatttttcac cccgtcgggc aaaaatacca
    13981 aaactcaaat caagccgttc ggaggcagct caaaaaacgg tacttcgcaa cagaagtacc
    14041 gtttatcggg attttaagtt ttattcttct aggcgttcgc cgtcggtttc gtccaatttt
    14101 ccttcggtga tgtgcatttc tacgccgttg agggcgcgaa tttttgcgtc gatttcgtcg
    14161 gcaatttcgg gattttcctt cagccagacg cggacgttgt ctttgccctg accgattttc
    14221 gcgccgttgt agctgtacca cgcgccggat ttgttgatga tgtcgttttt cacgccgatg
    14281 tcgattaatt cgccttccca actgatgcct tctccgtaga ggatgtcaaa ctctgcctga
    14341 cggaacgggg gggcgacttt gtttttgatg actttgacgc gggtttcgtt gcccaatact
    14401 tcttcgcctt ttttgatgga tccggtgcgg cggatgtcga ggcggacgga agaatagaat
    14461 ttcagcgcgt tgccgccggt ggtggtttcg gggctgccga acattacgcc gatcttcatc
    14521 cggatttggt tgatgaacac aaccagcgtg ttggtttttt tgatgtgtcc ggtcagtttg
    14581 cgcaaagcct gactcatcag gcgcgcctgc agtccgacat ggctgtcccc catatcgcct
    14641 tcgatttcgg ctttggggac gagtgcggct acggaatcga cgactaccat atctatgccg
    14701 cccgaacgga cgagtgtgtc gcagatttcc aaagcctgtt cgccggtatc gggctgggac
    14761 aggtaaagct cttcgacttt tacgccgagt ttgcgggcgt aaacgggatc gaaggcgtgt
    14821 tcggcatcga caaaggcgca cacgccgccg tttttctggc attgggcgac ggcttcgagg
    14881 cagagggtgg ttttgccgga ggattcgggg ccgaagattt cgacgatgcg cccgcgcggc
    14941 agaccgccga ctccgagggc gaggtctaat ccgagcgatc cggtggaaat gacttcgagg
    15001 ttttcttcct gctggctgcc gtccattttc atgatggcgc ctttgccgaa acttttttcg
    15061 atttgcgcca gtgcggcggc aagggctttg cttttgtcgt ctgacattgg ggttactccg
    15121 gaacaaatgc ggtatgtggg atacggcgca acacgggctg cggcgcggga tgtgtatcgt
    15181 tttcccgatg tgcgggctat cggtaatgct gcttcacggg gttgccatta tcgcatattt
    15241 ccttgcttgc cgatatgcgg caggacgcgg cggcttgtgc cggaatggaa tctggatgcc
    15301 gtctaaaagg cggccggctt tgttataatg gcggctgttt tttctgtgtg tgcctgtttt
    15361 atgtgttcct gccttgttgt caaaaatacc gttatcggaa gcggacgcac caaaatcgcc
    15421 gtgccgcttg tcgcccgcga tgccgccgta ctttccgccg tacttgacca aatcaaaaac
    15481 ctgcccttcg atattgcgga gttccgcgcc gattttttgg aatgcgcggg cagcatcggc
    15541 gaagtattgc accacacgca gaccgtccgt gacgcgctgc ccgacaaacc gctgctgttt
    15601 acgttcagac ggcatggcga aggcggctcg ttcccgtgtt cggacgatta ttattttaaa
    15661 ctgctcgacg cgctgatcga aagtcgcctg cccgacatta tcgatatcga gctgttttcc
    15721 ggcgaaaccg ccgtccggcg cgctgtggcg aatgctcaaa aaaacggcat cgccgccctg
    15781 ctctgcaatc atgagtttca ccgcacgccg ccgcaagaag aaatcgtatg ccgtctgaaa
    15841 cagatggagg actgcggcgc ggacatctgc aaaattgcgg tgatgccgca aagcgcggaa
    15901 gatgtgctga ctttgctttc tgccacgctc aaagcgaaac agcttgtcgc caaaccgatt
    15961 gttacgatgt cgatggggca aaagggggcg gtcagccgcc ttgccggaca aacgttcggc
    16021 tcaagcatca cattcggttc gggaacgcaa aactccgcac cggggcaaat cggcgtatcc
    16081 gccctccgtg cggcactcga ctgcctcgaa aacggagcag actgatttca gacagcatca
    16141 aaacatgatg aaactcaatc cccaacagct cgaagccgtc cgttacctcg gcggcccact
    16201 gctcgtcctt gccggtgcag gcagcggcaa aaccggcgta attactcaaa aaatcaatca
    16261 tttgattgtc aatgtcggct atctgccgca taccgtcgcc gcaattacct ttaccaacaa
    16321 agccgctgcg gaaatgcagg agcgcgtcgc caaaatgctg cccaaatcgc aaacgcgcgg
    16381 gctgacaatt tgcacgttcc attctttggg catgaagatt ctgcgcgaag aggcgaacca
    16441 tatcggttac aaaaaaaact tctccatcct cgattccacc gacagcgcca aaatcatcgg
    16501 cgaactctta ggcggtacgg gcaaagaagc tgtattcaag gcgcaacatc aaatctccct
    16561 gtggaaaaac gatctgaaaa cgcctgaaga tgtcgttcag acggcctcta atgcgtggga
    16621 acaacaaacc gcacgcgtgt atgcgagcta tcaggaaacc ttacaaagct atcaggcagt
    16681 ggacttcgac gacttaatcc gcctgcctgc cgtgctgttg cagcaaaaca gcgaggtgcg
    16741 caacaaatgg cagcggcggc tgcgttatct gctggttgac gaatgccaag atacgaatac
    16801 ctgccaattt acgttgatga aacttctgac cggcgcggaa ggtatgttta ccgccgtcgg
    16861 cgacgacgac cagtccatct acgcatggcg cggcgcgaac atggaaaacc tgcgcaaaat
    16921 gcaggaaaac tatccgcaga tgaaggtcat caaactggag caaaactacc gctccaccgc
    16981 gcggattctc aaaatcgcca acaaagtcat cgagaacaac cccaagctgt tcaccaaaaa
    17041 actttggtcg aaattgggcg aaggcgagcc cgtcaaagtc gttgcctgcc aaaacgagca
    17101 gcacgaagcc gactgggtcg tcagccaaat cgtcaaacaa aaactcatcg gcggcgacaa
    17161 aacccaatat gccgatttcg ccgtgttata ccgcggaaag catcaggcga ggattttcga
    17221 ggaagcattg cgcggtgcgc gcatccccta ccagctctcc ggcggacaaa gctttttcga
    17281 caaagccgaa atcaaagacg tgttgtctta tgtgcggctg cttgccaacc ccaacgacga
    17341 tcccgccttt ctgcgtgccg ttaccacgcc caaacgcggc atcggcgatg taacgctggg
    17401 caagctcaac acttacgcgc acgaacacga atgcagcctg tatgaagccg cgcaaaacga
    17461 agaagccctc gccacgctga acaataccaa ccgccaacac ctgcaagcct ttatggatat
    17521 gttcggcagt taccgtgcca aagccgaaac cagcgaagcg ggcgagttca tcaacagcct
    17581 gctcgaagaa atcgactatg aaaaccattt gatgcaaaac gaagaaggca aagccggcga
    17641 aatcaaatgg cgcaacgtcg gcgatttggt atcatggttt gcgcgaaaag gcggggaaga
    17701 cggcaaaaac atcatcgaac tcgcccaaac cgtcgccttg atgacgcttt tggaaggaaa
    17761 agacgaagaa gaaaccgatg ccgtctcgct atccacgcta cacgccgcca aaggtttgga
    17821 gtatccgtat gttttccttg tcggttgcga agaaggcgtt ttgccgcaca acgacagtat
    17881 cgaagagggc aacgtcgaag aagaacgccg cctgatgtac gtcggcatca cccgcgccaa
    17941 acgccaactc acgctgaccc actgcgtcaa acgcaaaaaa caaggcacat ggcagttccc
    18001 cgaacccagc cgattcatag acgaaatgcc gcaggaagat ttgaaaatcc tgggtcgcaa
    18061 aggcggcgag ccgattgtca gcaaagaaga aggcagacgc aaccttgccg atataatcgg
    18121 aaggctcgac aacctaaaaa aaagcggcgc ggcggattaa accggagccg caatgccgtc
    18181 tgaaggcttc agacggcata ttttttggac ggcgcgcgta aagcggttta cgcccacaaa
    18241 tcctgctgct ggtttttcgg cacaagatgc cccacgccga taccgataag gcggaacgcg
    18301 tcttccgtct gcggcgggac gcgcgccatc aacatttgcg cagcctgcag cagagctgcg
    18361 cagtcgggca atacggagga ataagtcagt gtgcgcgtga tgatgcggaa atcgtaggtc
    18421 ttcagcttga gcgttacgct ttgggcttcg acgtttttgc gcgtgatttg ccgccacaag
    18481 tcttcggcaa gatggggcag gtgtccggca gcctgctcga gcggcaggtc ttcgggcagg
    18541 gtaatttctg tggagatttg gaggcgttcg cgttcggctt tgacggggcg ttcgtccgta
    18601 ccgcgcacca aatcatagag gcggtatccg tagcgtccga aatggtttaa gagttcgccg
    18661 cgctcgaaac ggcgcaagtc gcccgccgtc cgcataccca gcgactgcat ttttttcagc
    18721 gttaccttgc ccacgccggg gattttgccc aaaggcaggg tttccaaaaa tgccatgact
    18781 ttgtgcggcg gcaacacaaa ctgcccgttc ggcttgcgcc agtccgacgc gattttcgcc
    18841 agaaatttgt tcggcgcgat gcctgcggat gcagtcaaac ctgtttccgc aaaaatggcg
    18901 gcacggattt ctttggcaac ttcactggcg taagggatgt ttttgaaatt acgggtaacg
    18961 tcaagatagg cttcgtccag cgacaagggt tcgattaaat cggtataacg cctgaatacg
    19021 gcgtgaatct gcgcggaaac ctgacggtac aaatcgaaat gcggcggcac atacaccgct
    19081 tgcggacaca gccttttcgc cgttgccacc gacatcgcgg aatgcagccc gaactgccgt
    19141 gcctcatacg atgcggcgca aatcaccgaa cgcgcgccct cccacgcgac gaccaccggc
    19201 cgccctttca aatgcggctg ttcgcgcagc tctaccgatg cgtagaatgc gtccatgtcg
    19261 atgtggatga ttttgcgtga agacatcggc tctgctgagg ataaaaagga tattctactg
    19321 ccggcatcgg gcaaatt
//
LOCUS       DAWFJW010000031        18290 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFJW010000031 DAWFJW010000000
VERSION     DAWFJW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.031"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(276..1379)
                     /locus_tag="WGR19_000874"
     CDS             complement(276..1379)
                     /locus_tag="WGR19_000874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0798426.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1614..3170)
                     /gene="dnaA"
                     /locus_tag="WGR19_000875"
     CDS             complement(1614..3170)
                     /gene="dnaA"
                     /locus_tag="WGR19_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0798427.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3462..3596
                     /gene="rpmH"
                     /locus_tag="WGR19_000876"
     CDS             3462..3596
                     /gene="rpmH"
                     /locus_tag="WGR19_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0798428.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3599..3964
                     /locus_tag="WGR19_000877"
     CDS             3599..3964
                     /locus_tag="WGR19_000877"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0798429.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            4029..4250
                     /gene="yidD"
                     /locus_tag="WGR19_000878"
     CDS             4029..4250
                     /gene="yidD"
                     /locus_tag="WGR19_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0798430.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4422..6059
                     /gene="yidC"
                     /locus_tag="WGR19_000879"
     CDS             4422..6059
                     /gene="yidC"
                     /locus_tag="WGR19_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0798431.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDEHKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLEGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCVAGDCSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRSDPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(6140..6850)
                     /locus_tag="WGR19_000880"
     CDS             complement(6140..6850)
                     /locus_tag="WGR19_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0798432.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAVVGLTDFVVEAEKTARA
                     HLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQR
                     SRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(6912..7502)
                     /gene="maf"
                     /locus_tag="WGR19_000881"
     CDS             complement(6912..7502)
                     /gene="maf"
                     /locus_tag="WGR19_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0798433.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7650..8153
                     /locus_tag="WGR19_000882"
     CDS             7650..8153
                     /locus_tag="WGR19_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0798434.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGSNGTPEKADRDKPNPFAV
                     LAGLKSN"
     gene            8187..8366
                     /gene="rpmF"
                     /locus_tag="WGR19_000883"
     CDS             8187..8366
                     /gene="rpmF"
                     /locus_tag="WGR19_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0798435.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8541..9068
                     /locus_tag="WGR19_000884"
     CDS             8541..9068
                     /locus_tag="WGR19_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0798436.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            9156..10211
                     /gene="plsX"
                     /locus_tag="WGR19_000885"
     CDS             9156..10211
                     /gene="plsX"
                     /locus_tag="WGR19_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0798437.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            10347..10598
                     /locus_tag="WGR19_000886"
     CDS             10347..10598
                     /locus_tag="WGR19_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0798438.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESQEISFINKEFEIESTSD"
     gene            10625..11041
                     /locus_tag="WGR19_000887"
     CDS             10625..11041
                     /locus_tag="WGR19_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798439.1"
                     /translation="MKTSTVVFGGFFITDNGERIQIPVLENPDIREINHFFSVSNFEK
                     KTGVLVFRIIPEPEFGNTELTIYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            11061..11264
                     /locus_tag="WGR19_000888"
     CDS             11061..11264
                     /locus_tag="WGR19_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798440.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            11291..12253
                     /locus_tag="WGR19_000889"
     CDS             11291..12253
                     /locus_tag="WGR19_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0798441.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAVEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVVGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            12300..12671
                     /locus_tag="WGR19_000890"
     CDS             12300..12671
                     /locus_tag="WGR19_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0798442.1"
                     /translation="MKRQAFFKPIACAAFLSAISLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIDYFVPPELFVKLGQHL"
     gene            12697..13623
                     /gene="fabD"
                     /locus_tag="WGR19_000891"
     CDS             12697..13623
                     /gene="fabD"
                     /locus_tag="WGR19_000891"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0798443.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            13762..15627
                     /gene="msbA"
                     /locus_tag="WGR19_000892"
     CDS             13762..15627
                     /gene="msbA"
                     /locus_tag="WGR19_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0798444.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLTPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15727..17292
                     /gene="guaA"
                     /locus_tag="WGR19_000893"
     CDS             15727..17292
                     /gene="guaA"
                     /locus_tag="WGR19_000893"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0798445.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLDGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            17354..18100
                     /gene="fabG"
                     /locus_tag="WGR19_000894"
     CDS             17354..18100
                     /gene="fabG"
                     /locus_tag="WGR19_000894"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0798446.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         4609 a         5525 c         4361 g         3795 t
ORIGIN      
        1 ttttggaatc aggcaaaacg cgtcttgcga aaattatagt ggattaacaa aaatcaggac
       61 aaggcgacga agccgcagac agtacaaata gtacgaaacc gattcacttg gtgcttcagc
      121 accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa
      181 tccactatac ctttccgaca gccgaacaaa accccgaatc cgtctgcacg gttcggggta
      241 tatctccaat acgggcatcg tgttccggaa aaccgtcaaa tccgcatcgg catcacaata
      301 tatttgaaat tcggattgtt cggcacggta aacagcgtcg agcggttggc atcgccgaag
      361 gcaagctgca tatcgtcgga atggatgttg cgcaacacgt ccatcagata gccgatattg
      421 aaaccgactt cgagttcgcc gccctgatag gcaatctcga tttcttcgcg cgcttcttcc
      481 tgctcgttgt tgctgcacac gacgctcaac aggccggatt gcaggaacag gcgcgcgcca
      541 cggaattttt cgttggcaag aatggacaca cgttccagcg cgcccaaaag ttcggcacgg
      601 gacaaaacga aaatcttgtc gttgtccaaa gggatgacgc ggttgaaatc ggggaatttg
      661 ccgtcgataa ccttgctgac gatggtcgtg ccgttgcatt ggaaacgcac ctgcttgtcc
      721 agcagctcga tttgaatcgg atcgtcgggg ttgttcaaca gtttgaacag ttccagcacc
      781 gttttgcgcg gcaaaatcac ttcggcgcgc ggcaaatccg catcaatcgc gcaggctgca
      841 taggcaaggc ggtgtccgtc ggtcgccaca aggcgcaact ggctgccctc aacctgcatc
      901 agcagaccgt tgagataata gcggatgtcc tgcaccgcca tgctgtactg cacttgcgac
      961 agcatggttt tgaaacgctc ctgctccagc gagaaagtcg cgctgatgtc ctcgccgaca
     1021 ttcatcatcg gaaaatcggc ggcaggcagg gtctgcaggg caaaacgcga tttgcccgcc
     1081 ttcagcgtca gacggctgtc gtcccaatcc agcgacacca gcgcaccggc aggcagtgcc
     1141 cgtaaaatgt cctgaaattt cttggcattg gtggtgatgc ggaagtcgtc cgcgccgccc
     1201 tcgggacccg cagtgtcgat ttggatttcc aaatcggttg ccaagagttt ggtctgaccg
     1261 ccttttccct caatcaggac gttggacagg atgggcaggg tgtggcggcg ttcgacaatg
     1321 ccggtaacgg cttgcaacgg cttgagcagg ctgtcgcgct cggcttgtaa aatcaacatg
     1381 ttcgctcctt taaatcggtt tgtatagtgg attaaattta aatcaggaca aggcgacgaa
     1441 gccgcagacg gtacaaatag tacggaaccg attcacttgg tgcttcagca ccttagagaa
     1501 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttaaagttaa tccgctatat
     1561 ctttaccctt cggacggcat gggcaatatc atgtcgtctg aaaacgtttt ccatcagttt
     1621 tgaatcagaa tcagcagctt ttcataatcc tgagccaatt ccggatcttc ttcgcgcagt
     1681 ttcgccactg ccctgatgcc gtgcataacg gtcgtatggt cgcgcccacc aaacgaatcg
     1741 ccgatagacg gcaggctcaa agtagtcagt tctttggtca ggctcatcgc cacctggcgc
     1801 ggacgggcaa tgttgcgcgt gcgttttttg ccgagtacgt cgctgatttt gatgcggtaa
     1861 tatttcgcca ccgcatcgat gatgatgtcg gcggtgatga ctttgtgctt ctcggcaata
     1921 atgtcctgca aagcggtacg cgccaaatcg atgtcgatga cgggacggtt cataaagcgg
     1981 ctgctcgctc cgacacgatt aaacgcgcct tcaagctcgc gcacgttgga acggatcaga
     2041 ttggcaatga acagcgcggc ttcgtcttcg atactgatgc ccgccgcttc cgcctttttc
     2101 tgcaaaatgg cgatgcgcat ttccaattcg ggcggctcga gttccaaagt cagtccccat
     2161 gaaaaacggg atttgaggcg gtcgtccatg ccttcgattt tcgcaggcaa cacatcgcaa
     2221 gtgaggatga gctgtttttt ctcgttgtgg aaatggttgt acagatagaa aaactcttcc
     2281 atcgtacggt ctttgccttt gatgaactgg atgtcgtcga taatcagcag gtcgtattgc
     2341 ttgtattgct gcttgaacac gtcgtaagtg ttgttgcgaa ccgccttcat aaagctgcgg
     2401 atatagtcat ccgaatgcat atagcgcact ttggcatcgg gacggttttt cagcagctcg
     2461 ttgccgaccg cctgcacaag gtgggttttg cccaaacccg tactgccata gaggaagaac
     2521 gggttgtaac tctgccccgg gctttccgca atcgcctgcg ccgcagccgc cgcaaggcgg
     2581 ttgcccttac cttctaccaa cgtatcaaac gtgtaatccg gagacaggtt ggtctgctcg
     2641 taacgcgcct cttccgcatc gcgctgcacg tccgtccgtg ctttggcaac tgccaccgat
     2701 tccggccggg aagcagaccc ggcagcctga cgcggctcgt gcggcaggtt tttcatacgt
     2761 tccgccaaaa tatccgccgc cgttttcgac gcagcgggtt tgacaggctc ttcagacggc
     2821 agctcgtcca acagaacctc ctgcacgggc attccctccg acaccgcatg caaggacggc
     2881 tcggcaggtt cgacagcacc ttcaaccgcc gccatctcat aacgcacgcc ttctcccggt
     2941 ttgaatacga aggcggaacg gccggcagcc aactcttccc tcaccgcttc tatttttccg
     3001 gcaaactggc tcttgagcat attgcaggca aactggttct tgccgtacac cacccatacg
     3061 ccaccctcct caccaacggt aaggggcgca atccattgcg caaactgccc ttgaggcaac
     3121 atatcgtgaa gacggcggag gcacagcggc caaaactctg ctaatgtcat ggataggctc
     3181 gaatcggtaa aaatgaaatc gaaaacaaag aaaatataat attttcaaaa agaaaacaaa
     3241 tctgttgaac gcacatcggt tcaaaacgcg actgcccgat tataccgact cacgaatatt
     3301 ttatccacaa cccgtgcaaa aatttatcca cagaaaggcg gcggaaatcc gcaggcaatc
     3361 gggcaatctt cctgcaaagt ttctatattg attgacaaaa gcggcaaatt ggagtgtaat
     3421 tcacggttta attatctacc cattctattt taggaaacat catgaaacgc acttatcaac
     3481 cttccgttac caaacgcaaa cgcacccacg gcttcctggt gcgctccaaa acacgcggcg
     3541 gccgcgcagt attggctgca cgccgcgcca aaggccgcaa acgcctggcg gtataatttt
     3601 ggactaccgc ttcggaaggc agtaccgctt gttaaaaacg gatgattttt catccgtttt
     3661 tgcattcaga aaccgccgca gccgcgacct gctgcaagtt tcgcgctcaa acggcaacgg
     3721 gctggatcat ccccgcatcg gtctggtggt cggcaaaaaa accgccaaac gcgccaacga
     3781 acgaaattat atgaagcgcg ttatccgcga ctggttcaga ttgaacaaaa accggctgcc
     3841 gccgcaggat ttcgtcgtgc gcgtccgccg taaattcgac agggctaccg caaaacaggc
     3901 aagggcggaa ctggcacaac tcatgttcgg caaccccgca accggatgcg ggaaacaggt
     3961 atgatcagaa cggtactctg caggcaaggt tcagacggca atgggtttcc catacaagga
     4021 acatcccgat gaacttccta ttgtccaaac tcctgctggg actgatacgg ttctaccaat
     4081 attgcatcag cccgctgatt ccgccgcgct gccgctatac gccgacctgt tcgcaatacg
     4141 cggtcgaagc ggtcaaaaaa tacggcgcat tcaaaggcgg ccggctcgcc atcaagcgca
     4201 ttgcacgctg ccaccctttc ggcggacacg gacacgaccc cgttccctga cccgacgcaa
     4261 tattcaaatt gcacgctttc cttttatttc ccatcggttt ctatataatg ccgtctgaaa
     4321 cttcgggctg gcggcacgac cgtcgggtat gaagcccgcc cttattctcc gtctatcgga
     4381 acacgcaacc tgcggcattt ccgaccattc aggaaactct tatggatttt aaaagactca
     4441 cggcgttttt cgccatcgca ctggtgatta tgatcggatg ggaaaagatg ttccccactc
     4501 cgaagcccgt ccccgcgccc caacaggcag cacaacaaca ggccgtaacc gcttccgccg
     4561 aagccgcgct cgcgcccgca acaccgatta ccgtaacgac cgacacggtt caagccgtca
     4621 ttgatgaaaa aagcggcgac ctgcgccggc tgaccctgct caaatacaaa gcaaccggcg
     4681 acgaacacaa acccttcgtc ctgtttggcg acggcaaaga atacacctac gtcgcccaat
     4741 ccgagctttt ggacgcacaa ggcaacaacg ttctgaaagg catcggcttt accgctccgc
     4801 aaaaacaata cagtttggaa ggcgacaaag ttgaagtccg cctgagcgca cctgaaacca
     4861 acggcctgaa aatcgacaaa gtttatactt tcaccaaagg cagctatctg gtcaacgtcc
     4921 gcttcgacat cgccaacggc agcggtcaaa ccgccaacct gagcgcggac taccgcatcg
     4981 tccgcgacca cagcgaaccc gagggtcaag gttactttac ccactcttac gtcggtcctg
     5041 ttgtttatac ccctgaaggc aacttccaaa aagtcagctt ctccgacttg gacgacgatg
     5101 ccaaatccgg caaatccgag gccgaataca tccgcaaaac cccgaccggc tggctcggta
     5161 tgatcgaaca ccactttatg tccgcttgga ttttgcaacc caaaggcgga caaagcgttt
     5221 gcgtcgctgg cgactgcagc atcgacatca aacgccgcaa cgacaagctg tacagcgcaa
     5281 gcgtcagcgt tccccttgcc gccatcccgg ccggcgcgaa agccgaaacc gccgtcaacc
     5341 tgtatgccgg cccgcaaacc acttccgtca tcgcaaacat cgccgacaac ctgcaactgg
     5401 ccaaagacta cggcaaagta cactggttcg cttccccact cttctggctc ctgaaccaac
     5461 tgcacaacat catcggcaac tggggctggg caatcgtcgt tttgaccatc atcgtcaaag
     5521 ccgtactgta tccattgacc aacgcctctt accgctctat ggcaaaaatg cgtgccgccg
     5581 cacctaaatt gcaagccatc aaagagaaat acggcgacga ccgtatggcg caacaacaag
     5641 cgatgatgca gctttacaaa gacgaaaaaa tcaacccgct gggcggctgt ctgcctatgt
     5701 tgttgcaaat ccccgtcttc atcggcttgt actgggcatt gttcgcctcc gtagaattgc
     5761 gtcaggcacc ttggctgggc tggattaccg acctcagccg ttccgacccc tactacatcc
     5821 tgcccatcat tatggcggca acgatgttcg cccaaaccta tctgaacccg ccgccgaccg
     5881 acccgatgca ggcgaaaatg atgaaaatca tgccgttggt tttctccgtc atgttcttct
     5941 tcttccctgc cggtctggta ttgtactggg tagtcaacaa cctcctgacc atcgcccagc
     6001 aatggcacat caaccgcagc atcgaaaaac aacgcaccca aggcgaagtc gtttcctaaa
     6061 tgccgcagca tgaaaaatgc cgtctgaaac ctgttcagac ggcattttta ttgttcaccc
     6121 cctatcgggg cggaaatctt caacctgcat acatcacaaa aatcgtcggg cgttttttca
     6181 gattgggcat ttccttcatc ctgcgccaat ccgcaaccgt tttactgacc accgactgag
     6241 tcggcaacgt caaatccgta gccgtgcaca agcgcgtttc aggatgcagg ttttccaccg
     6301 catccgccaa caacacatcg ttgcgatacg gcgtttcgat aaacagctgc gtttcattct
     6361 gctggcgcga acgttgctcc aaagccttca aactctgaat ccgctcgttt ttttcagacg
     6421 gcagataacc cttaaacgca aaactctgcc cgttcgcacc cgaagccatc aaagccagca
     6481 gcaggctgga aggcccaacc agcggacgca cttcaaaacc gtgtttatgc gccaaagcca
     6541 ccagattcgc acccggatcg gcaacagccg ggcaacccgc ctcgctgaca atgcccatac
     6601 tgcgcccttc ctgcaaaggt ttcagcaatt ccggcaaagt cttcaaatcc gtgtgttcgt
     6661 tcagcgtttg caaattcagt tcgcggatag gcattgtcac gcccaaatgt ttcaaatgcg
     6721 cgcgcgccgt tttttccgct tccacgacaa aatccgtcag cccgacaacc gcctgttgtt
     6781 catgcggcaa caggcacggc gtatcgggcg tacccaaagg cgtaggaatc aaatacaaaa
     6841 caggagacat cattccctca ctcatcggtt aaaaatgccg tctgagcctt tcagacggca
     6901 taaacgggca gttacagaac ctccacgccc tcatttttca agaaatcgac cagacggaaa
     6961 accggcaaac cgattaaagc attcggatcg gtactctcaa tccgttcgat caacaatgca
     7021 cccaaagcct cgctcttcaa cgcacacgag caataaaccg catcaggctc gcgctccaaa
     7081 tagcggagga tgtgcagctc gtccaacttc ctcatcacga ccaccgtctt atcgatatgc
     7141 cgctgcatcc tgcccgtaac cgtatttagc agaacgaccg cgctgtaaaa ctcaatctcc
     7201 ctgccgctca aatgcatcag catcttttgc gcgttggcaa ggttcatcgg cttgccccac
     7261 tgcctgccgt cgcaccacgc cacctggtcc gcaccgacaa tcaacgcctc ggggaaacgt
     7321 ccggccaacg acctcgcctt accctcggca aggcgcaatg ccgtctgagg ggcggattcc
     7381 cccaacatcg gcgtttcgtc aaaatcgggg gacgccgcct gaaaggcaat gccgagcctt
     7441 tccatctgtt cgcggcggaa aaccgaactc gtacccaaaa tcaaaggcag ttccaaaccc
     7501 atcccatcct ccttaccgtt gaaaacacgc ccgaaggggc ggtaaaatcc agccatgcgc
     7561 cgaaacacgg atacccgcct tcggcgtacc gcaacatttt tcttaaaaat attgacgtta
     7621 gaacatctaa attatatcat atctcgttta tgtcagaccc taatttgatt gacccggaaa
     7681 ttttcgccgc cgaagggcag aatctgcaag gcagttttct gctggaagaa ttggatgaac
     7741 gcgtcagttc gcacgattat cccgccgaca ggcagaccaa aatatcgttt acactgaccg
     7801 gcggtcgcga tcggctgcaa cgcctgttcc tcgacctgaa cgtcaaagcc gatatgcccc
     7861 tgatttgcca gagatgtatc aaacccatgc cgttcatgct tgatgaaagc agccgtatcg
     7921 tcctgttttc caacgaagag tccttggacg aatccatgct tgccgacgaa gaactcgaag
     7981 gcatactgat tgaaaaagaa ctcgacgtgc gcacattggt agaagaccaa atcctgatgt
     8041 cgctcccctt ctctccccgg cacgaagact gcggcagcaa cgggacaccg gaaaaagccg
     8101 atcgggacaa accaaacccc ttcgccgttt tggcaggttt gaaaagcaat tgattaggac
     8161 acagtttatt tatccaggag cttgaaatgg ccgttcaaca aaacaaaaaa tccccttcca
     8221 aacgcggtat gcaccgttcg cacgacgcgc tgaccgcgcc cgcactgtct gtcgacagca
     8281 caaccggcga agtacaccgc ccgcaccaca tctcccccaa cggtatgtac cgcggccgca
     8341 aagtggtcaa agccaaaggc gaataatccc tattcgactg actgaaaaag ccagaacatt
     8401 gccatgcaat tactggcttt ttttgcattg gacgcaccat ccgtccaaac tttcgccata
     8461 cgtcaacaca caggggcaaa gcgttccgta taataccccg tgaaaatatt ccaaaagccc
     8521 caaccaccaa ggaaattccg atgaaacaga aaatctggta cacctacgat gacatccacc
     8581 gcgtcatcaa agcattggca gaaaaaatcc ggaacgccga catcaaatac gatgccatga
     8641 ttgccatcgg cggcggcggc tttattccgg cacgtatgct gcgctgtttt ctggaaattc
     8701 cgatttatgc cgtaaccacc gcctattacg acagcgacaa cgaaggacag gttacagaag
     8761 aagtcaaaaa agtccaatgg ctcgaccccg ttcccgaagc cctgcggggc aaaaacgtcc
     8821 ttgttgtcga tgaagtggac gacagccgcg taaccatgga attttgcctg acagagctgc
     8881 ttaaagaaga cttcggcacc atcggcgtcg ccgtactgca cgaaaaaatc aaagccaaag
     8941 caggtaaaat ccccgaaggc attccctatt tcagcggcat caccgtagaa gactggtgga
     9001 tcaactatcc atgggacgca ctcgacatcg acgaacacaa ccgccttgcc gaggccggcc
     9061 gaggctgacc ctttcagacg gcatattttc cgaaccgatg ccgtctgaag cccgcacgac
     9121 ccctgccgca gaccgaaaac ctaccggaga accctatgat tacacttgcc gtagatgcca
     9181 tgggcggcga ccaaggactt gccgttaccg tacccggcgc aaccgcattc ctccaagcac
     9241 accccgatgt ccgcctgatt atgaccggcg acgaaacgca actgcgccaa gccctgaccg
     9301 cggcaggcgc accgatggaa cgcatcgaca tctgccacac cgcacaaatc gtcggcatgg
     9361 acgaagcgcc gcaatccgcc ctaaagaaca aaaaagactc ctccatgcgc gttgccatca
     9421 accaagttaa agaaggcaaa gcccaagccg ccgtatccgc aggcaacacg ggcgcgctca
     9481 tggcaaccgc acgtttcgtc ctcaaaacca ttcccggcat cgaacgcccc gccatcgcca
     9541 aattcctccc gtccgacact gaccatgtta cccttgcact cgacctcggc gcgaacgtcg
     9601 actgcacccc cgaacagctc gcccaatttg ccgttatcgg cagcgaactc gtccacgcac
     9661 tccatcctca aaaaggacag ccgcgcgtcg ggctggtcaa cgtcggcacg gaagacatca
     9721 aaggtacgga caccgtcaaa caaacctaca aactgctgca aaacagcaaa ctcaacttta
     9781 tcggcaacat cgaaagcaac ggcatcctgt acggcgaagc agatgtcgtc gtcgccgacg
     9841 gctttgtcgg caacgtcatg ctcaaaacca tcgaaggcgc ggtcaaattc atgagcggag
     9901 ccatccgccg cgaattccaa agcaacctgt tcaacaaact tgccgccgtt gccgccctac
     9961 ccgccctcaa agggctgaaa aacaaactcg atccgcgcaa attcaacggg gccatcctgc
    10021 tcgggctgcg cggcatcgtc atcaaaagcc acggcggcac agacgaaacc ggtttccgct
    10081 acgcactcga agaagcctac cacgaagcca agtccgccgg cctttccaaa atcgaacaag
    10141 gcgttgccga acaactcgcc gcactcgaaa ctgccaaagc cgtccaaaac gaaaatgtcg
    10201 gcggtctgta acacacacga tgccgtctga acgcccccgc ccctttcaga cggcattcgc
    10261 ccgcaccaaa cctgcgggcg cggacggcga tgcgcctgtc cggcacttcc caaatatcgc
    10321 cttgtaaaat aaggagtatt tgaaaaatga agacattaga aaaacggatg aaagctctag
    10381 acaaacggat gatgaagttc ggaaaatccc ttgaaggcag gcttgatgcc cgtctgattg
    10441 aatccgcatt ggattatatt cattattcgg aacgtttttt ggcttttgaa atcctgtgta
    10501 cttatatcga agatttcgat gtccggctga cggaacaaga atcccaagaa atttctttta
    10561 tcaacaagga atttgagata gaaagcacgt ccgattaacc aataaagcca atgatttgat
    10621 aagaatgaaa acatcgacgg tcgtttttgg cggatttttt attacagata atggagagcg
    10681 aatccaaatc cccgttttgg aaaatcctga cattagggaa atcaatcact ttttttccgt
    10741 atcaaatttt gagaaaaaaa ccggcgtcct tgttttcaga atcatccccg agccggaatt
    10801 tggcaatacc gaattaacta tctattttga aaaaggatat tatttgccaa tgattcaaac
    10861 aattttagaa ggcggggata ttgaagtcaa aaaccttaaa acggaaaatt atagtggaaa
    10921 tacaatggaa atttggggag attcctaccc tattgagcat atctctaaaa atatttctac
    10981 aattcaaaat ataatttctg aatttatgat acaaaaacaa acaccggcac caataatttg
    11041 atggaaaccc aacaatcaaa atgccgtctg aagcatgttt ggttttcaga cggcatttcc
    11101 ttcgcttaaa acagcgtatc ggcaaccccg ccctgcccgt ccacggcaat ctgcatctga
    11161 aaaccatctg tatcccaaac cacaccccca tcccgatttc cgtcatttgc accctgcccg
    11221 tattgggcaa tcatcggttt ttcgcttaca atagccgaat ctgaaccaac tatccaaaaa
    11281 ggccgttccc atgcagtatg ccaaaatttc cggcacgggc agctatcttc ccgccaaccg
    11341 cgtcagcaat gacgaccttg cccaaaaagt ggatacctcg gacgagtgga ttaccgcgcg
    11401 cacgggcatc aagttccgcc atattgcagc cgaaaatgag aaaaccagcg atcttgccgt
    11461 cgaagcagcc cgccgcgcgc tggatgcagc cggattagac agcggcgaaa tcgatttgat
    11521 tatcgtggcg acggcaacgc cggatatgca gtttccttct accgccacca tcgtgcagca
    11581 aaaattgggc atcaccaacg gctgtcccgc ctttgacgtg caggcggtgt gtgcgggctt
    11641 tatgtacgcg ctgacgacgg caaacgccta catcaaaagc ggtatggcga aaaacgcgct
    11701 ggtcgtcggc gcggaaacct tcagccgcat cgtggattgg aacgaccgca ccacctgcgt
    11761 attgttcggc gacggcgcgg gcgcggtggt tttgagcgcg gcggacaaac cgggcatcat
    11821 ccacagcaaa ctcaaagccg acggcaatta tctgaaactc ttaaacgtcc ccgggcaaat
    11881 cgcctgcggc aaagtttccg gttcgccgta catttcgatg gacggtcccg gcgtgttcaa
    11941 gtttgccgtc aaaatgctgt ccaaaatcgc cgatgacgtt atcgaagaag ccggttatac
    12001 cgccgctcaa atcgactgga tcgtgccgca tcaggcaaac cgccgcatta tcgaatcgac
    12061 cgcgaaacat ttaggtttga gtatggacaa agtcgtcctg accgtccaag accacggcaa
    12121 cacatccgcc gcatcgattc cgctggcttt ggatacgggc atccgcagcg gacaaatcaa
    12181 acgcggtcaa aacctgctgc tcgaaggcat cggcggcggt ttcgcgtggg gcgcggtatt
    12241 gctgcaatat tgaataccat gccgtctgaa gcaggttttc agacggtatt tcccatatca
    12301 tgaagcggca ggctttcttc aaaccaattg cgtgtgcggc atttctgtct gccatttcgc
    12361 tgcgcctccc cgtattgggc gcgtgttacg caatattgtc cctctatgcg tttgcacttt
    12421 acggcatcga caaacggcgt gccgtgcggg gaaaacgccg cattcccgaa caccgcctgc
    12481 tgctgcctgc cttgttcggc ggttgggcgg gcgcatactt gggcagcagg atattcaggc
    12541 ataaaacggc gaaaaagcgt tttgttgtgc tgttccgtct gactgtttca ggtaatgtcc
    12601 tggcgaccct catcctgatt gattatttcg ttccgcccga actttttgtc aaactcgggc
    12661 aacatctctg acaatccaac caaacaaagg aataccatgt cttttgcatt cttctttccc
    12721 ggacaaggtt ctcaaagcct cggcatgatg aacggcttta cagggcaggc cgtcgtcaaa
    12781 aacacatttg acgaagcatc cgccatattg ggtcaagact tgtgggcgat gattaacggt
    12841 agcgatgccg aaatcatcgg tcaaaccgtc aatacccaac ccatcatgct cgccgccggc
    12901 gttgccgttt accgcgccta tttggaagcg ggcggcaaaa cgcctgccgc cgttgccgga
    12961 cacagcctcg gcgaatatac cgcactcgtt gccgccggcg cattggattt tgccgacgcg
    13021 gtcaaactcg tgcgcctgcg cgccgaactg atgcagtctg ccgtaccgca aggcgtgggc
    13081 gcaatggcgg cgattctcgg cttggaagat gaacaggtaa aacaaatttg cgccgaatcc
    13141 acacaaggcg aagtggttga agccgtcaac ttcaactcgc ccggacaagt cgtgattgca
    13201 ggcaataccg ccgccgtcga gcgcgctatg cttgccgcca aagaagccgg cgccaaacgc
    13261 gccctgccgc tgcccgtgtc cgtaccttcc cattgcagcc tgatgaaacc cgccgccgac
    13321 aaactcgccg aagccctgaa aaccgttgaa atcaagcagc cgcaaatccg cgtcatccac
    13381 aacgccgacg ttgccgccta cgatgatgcc gacaaaatca aagacgcgct cgtccgccag
    13441 ctttacagcc ccgtacgctg gacagaaacc gtcaacgccc ttgtttcaga cggcattgcc
    13501 gaatccgccg aatgcggccc gggcaaagtg ttggcgggct tggcaaaacg catcaacaag
    13561 gccgccgcgt gcagcgcact gaccgatgcc ggacaggttg ccgcctttat cgaagcgcac
    13621 tgacttcgtt ccgcaaaaag cagcctgccc tcttcaggct gcttttcacg tccgaacggc
    13681 aaacagccgc atatttgcgc tataattgat gacgaccaaa ccaccgacag cggctgccgt
    13741 tgcagttccc gccctaccga tatgatagaa aaactgactt tcggactgtt taaaaaagaa
    13801 gacgcgcgca gctttatgcg cctgatggcg tacgtccgcc cctacaaaat ccgcatcgtt
    13861 gccgccctga ttgccatttt cggcgttgcc gccaccgaaa gctaccttgc cgccttcatc
    13921 gcccccctga ttaaccacgg cttttccgca cctgccgcgc cgcccgagct gtctgccgcc
    13981 gccggcatca tttccaccct gcaaaactgg cgcgaacagt ttacctatat ggtttggggg
    14041 acggaaaaca aaatctggac cgtccccctc ttcctcatca tcctcgtcgt catccgtggc
    14101 atctgccgct ttaccagcac ctatctgatg acttgggtct ccgtgatgac catcagcaaa
    14161 atccgcaaag atatgtttgc caaaatgctg accctttcct cccgctacca tcaggaaacg
    14221 ccgtccggca ccgtactgat gaatatgctc aacctgaccg aacagtcggt cagtaacgcc
    14281 agcgacatct tcaccgtcct cacgcgcgac acgatgatcg ttaccggcct gaccatcgtc
    14341 ctgctttacc tcaactggca gctcagcctc atcgttgtcc tgatgttccc cctgctctcc
    14401 ctgctctcgc gctactaccg cgaccgtctg aagcacgtca tttccgactc gcaaaaaagc
    14461 ataggcacga tgaacaacgt gattgccgaa acccatcagg gacaccgcgt cgtcaagctg
    14521 ttcaacgggc aggcgcaggc ggcaaaccgg tttgacgcgg tcaaccgcac catcgtccgc
    14581 ctcagcaaaa aaatcacgca ggcaacggcg gcacattccc cgttcagcga actgattgcc
    14641 tcgattgccc tcgccgttgt catcttcatc gccctgtggc aaagccaaaa cggctacacc
    14701 accatcggcg aatttatggc attcatcgtc gcgatgctgc aaatgtacgc acccatcaaa
    14761 agccttgcca acatcagcat ccctatgcag acgatgttcc tcgccgccga cggcgtatgt
    14821 gcatttctcg acaccccgcc cgaacaggac aaaggcacgc tcacaccgca gcgtgtcgaa
    14881 gggcgcatca gcttccgcaa cgtcgatgtc gaataccgtt cagacggcat caaagccctc
    14941 gacaacttca atctcgacat cagacaaggc gaacgcgtcg ccttggtcgg acgttccggc
    15001 agcggcaaat ccaccgtcgt caacctgctg ccccgctttg tcgaaccgtc tgccggcaac
    15061 atctgcatag acggtatcga catcgccgac atcaaactcg actgcctgcg cgcccaattt
    15121 gccctcgtct cccaagacgt attcctgttt gacgacaccc tgtttgaaaa cgtccgatat
    15181 agccgtcccg acgcgggcga agccgaagtc ctgtccgccc tccaagccgc taacctgcaa
    15241 agcctgatcg acgcatcacc gctcggactg caccagccca tcggatcgaa cggcagcaac
    15301 ttatccggcg gacagcggca acgcgttgcc attgcccgcg ccattttgaa agacgcgccg
    15361 atattattat tggacgaagc caccagcgca ttagacaacg aatccgaacg cctcgtccaa
    15421 caggcgctcg aacgcctgat ggaaaaccgc accggcatca tcgtcgccca ccgcctgacc
    15481 accatcgaag gggccgaccg catcatcgta atggacgacg gcaaaatcat cgaacaaggc
    15541 acacacgaac aactgatgtc ccaaaacggc tactacacaa tgttacgcaa tatctcaaac
    15601 aaagatgccg ccgtccggac ggcataaaca aaatgccgtc cgaaatggta caatcgcccc
    15661 gaccctttca gacggcatca tatccgccga cccatccgat tatcttcaat cactgtaaaa
    15721 cccattatga cccaagacaa aatcctcatc ctcgacttcg gttctcaagt tacccagctg
    15781 attgcccgcc gcgtgcgcga agcccacgtt tactgcgaac tgcattcctt cgatatgcct
    15841 ttggacgaaa tcaaagcctt caaccccaaa ggcatcatcc tctctggcgg ccctaattcc
    15901 gtttacgaat ccgactatca agccgatacc ggtatctttg acttgggcat tccgattttg
    15961 ggcatttgct acggcatgca atttatggcg caccacttgg atggtgaagt gcaacccggc
    16021 aaccagcgcg aattcggtta cgcgcaagtc aaaaccatcg acagcgagct gacacgcggc
    16081 attcaagacg atacgccaaa cacgctcgac gtatggatga gccacggcga taaagtttcc
    16141 aaactgccta ccggtttcac cgtcatcggc gacacgccgt cctgcccgat tgccatgatg
    16201 gaaaacgccg aaaaacaatt ctacggcatc caattccacc ccgaagtaac ccacaccaaa
    16261 caaggccgcg ccctgttgaa ccgctttgtt ttggatattt gcggcgcaca accgggctgg
    16321 acaatgccga actacatcga agaagccgtt gccaaaatcc gcgaacaagt cggcagcgac
    16381 gaagtgattt taggcttgtc cggcggcgtg gactcttccg tagccgccgc gctgatccac
    16441 cgcgccatcg gcgaccaact gacctgcgtg ttcgtcgatc acgggctctt gcgcctgaac
    16501 gaaggcaaaa tggtgatgga tatgttcgcc cgcaacttgg gcgtgaaagt gatacacgtc
    16561 gatgccgaag ggcagtttat ggcgaaactc gccggcgtga ccgaccccga gaaaaaacgc
    16621 aaaatcatcg gcgcggaatt tatcgaagta tttgatgccg aagagaaaaa actcaccaac
    16681 gccaaatggc tggcgcaagg cacgatttac cccgacgtaa tcgaatccgc aggcgcgaaa
    16741 accaaaaaag cccacgccat caaatcccac cacaatgtcg gcggcctgcc tgaaaacatg
    16801 aagctcaagc tgcttgagcc gttgcgcgac ttgtttaaag acgaagtgcg cgaactgggc
    16861 gttgctttgg gtctgccgcg cgaaatggtg taccgccatc cgttccccgg ccccggtttg
    16921 ggcgtgcgca tcttgggcga agtgaaaaaa gaatacgccg acttattgcg tcaagcagac
    16981 gatattttca tccaagaatt acgcaacacc accgacgaaa acggcacatc ttggtacgac
    17041 ctgaccagcc aggcatttgc cgtattcctg cctgtgaaat ccgtcggcgt aatgggcgac
    17101 ggacgcactt acgactacgt cgtcgcactg cgcgcagtca tcaccagcga cttcatgacc
    17161 gcacattggg ctgagttgcc ttactcactg ctcggacgcg tgtccaaccg catcatcaac
    17221 gaagtcaaag gcatcaaccg cgtggtgtac gatgtcagcg gcaaaccgcc tgccaccatc
    17281 gagtgggaat aaccagcaaa cagggctgca ccgtccggcg cagtctttcg attatcggaa
    17341 aaaaggaaaa aatatgagca cacaagattt aagcggcaaa atcgctttgg taaccggcgc
    17401 atcgcgcggt atcggtgcag caattgccga cacactggcg gcagccggtg ccaaagtcat
    17461 cggtacggcg accagtgaga gcggtgcggc ggcgattagc gagcggttgg cgcaatgggg
    17521 cggcgaaggc cgcgtattaa attccgccga acccgaaacc gtcgaaaacc tgattgccga
    17581 catcgaaaaa acgttcggca aactcgatat tctggtcaac aacgccggca tcacccgcga
    17641 caacctcctg atgcgtatga aagaagaaga gtgggacgac atcatgcagg tcaacctcaa
    17701 atccgtcttt cgcgcctcca aagccgtctt gcgcggcatg atgaagcagc gcgccggccg
    17761 tatcatcaac atcacatccg tcgtcggcgt gatgggcaat gccggtcaaa ccaactatgc
    17821 cgcggcaaaa gcaggcttaa tcggtttctc caaatccatg gcgcgcgaag tcggcagccg
    17881 gggcattacc gtcaactgcg tcgcccccgg ctttatcgat accgacatga cccgcgccct
    17941 gccggaagaa acccgccaaa cctttaccgc ccaaaccgcc ttgggcagat tcggcgacgc
    18001 gcaagacatt gccgatgcag tcttgttctt ggcttccgat caggcaaaat acattaccgg
    18061 tcaaacgctg cacgtcaacg gcggcatgct gatgccctga cgggcagccg taccgacaat
    18121 gccgtctgaa gccctttcag acggcatttg cattctcagg caaaatgaac acacgccaca
    18181 ccccgccctg cccctgcgga tcaggcacaa gctgagacct ttgcaaaact cctttccctc
    18241 ccgacagccg aaacccaaac acaggttttc gtctattttc gccccaaata
//
LOCUS       DAWFJW010000032        17986 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFJW010000032 DAWFJW010000000
VERSION     DAWFJW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.032"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(479..3268)
                     /gene="ileS"
                     /locus_tag="WGR19_000895"
     CDS             complement(479..3268)
                     /gene="ileS"
                     /locus_tag="WGR19_000895"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0798447.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGNKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMRVWEANPVILQWLEETGNLLA
                     NGKIEHSYAHCWRHKTPLIYRATGQWFIGMDKAGNDGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVLKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKEMVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGAEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGHETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3411..4331)
                     /gene="ribF"
                     /locus_tag="WGR19_000896"
     CDS             complement(3411..4331)
                     /gene="ribF"
                     /locus_tag="WGR19_000896"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0798448.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFASRTGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDRHF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVGGGRFQKLEVHLFDFQGDLYGQGLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            complement(4858..6153)
                     /gene="tyrS"
                     /locus_tag="WGR19_000897"
     CDS             complement(4858..6153)
                     /gene="tyrS"
                     /locus_tag="WGR19_000897"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0798449.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(6216..7997)
                     /locus_tag="WGR19_000898"
     CDS             complement(6216..7997)
                     /locus_tag="WGR19_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0798450.1"
                     /translation="MAAGRVSRCRYILCDFRLFDDDDSSPRNVGGGGRFSLKAFYIRR
                     IKRILPAFFAVLAATLTGGFFLFTKDDFFLLWKSALTALGFASNLYFARGKDYFDPAQ
                     EEKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFGFLAALCALSLAASFMPSAFD
                     KYYLPHLRACEMLVGSLTAVWMRCRQPAVGRRCAAAGALFAVCILSTCLFSYSEQTAY
                     FPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYLWHWPILAFMRY
                     IGPDNLPPYSPAAAIVLTLAFSLISYHCIEKPFKKWKGSFAQSVLWIYALPMLILGAG
                     SFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGDSHADHYKTF
                     FDAVGKKEKWSATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLPLYSKVVLAM
                     RWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLASSYNVQRAYILSS
                     RIPGYRQTLRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPANFQIGGLPVYSDKD
                     HINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(7886..8116)
                     /locus_tag="WGR19_000899"
     CDS             complement(7886..8116)
                     /locus_tag="WGR19_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0798451.1"
                     /translation="MRRRDESKNIMSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPG
                     GFLGVDIFFVISGFLMTMILHREMSGGGGGFP"
     gene            complement(8356..9447)
                     /gene="ychF"
                     /locus_tag="WGR19_000900"
     CDS             complement(8356..9447)
                     /gene="ychF"
                     /locus_tag="WGR19_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0798452.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDHLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9561..11237)
                     /locus_tag="WGR19_000901"
     CDS             complement(9561..11237)
                     /locus_tag="WGR19_000901"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0798453.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKNISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPVCMAKTQYSLSDNAKLLGCPEDF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            11412..11843
                     /locus_tag="WGR19_000902"
     CDS             11412..11843
                     /locus_tag="WGR19_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0798454.1"
                     /translation="MGSNVWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(12088..12783)
                     /locus_tag="WGR19_000903"
     CDS             complement(12088..12783)
                     /locus_tag="WGR19_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0798455.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSMQPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVD
                     RLKEAQAVAGAWK"
     gene            complement(12799..13299)
                     /gene="hpaC"
                     /locus_tag="WGR19_000904"
     CDS             complement(12799..13299)
                     /gene="hpaC"
                     /locus_tag="WGR19_000904"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0798456.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(13414..13854)
                     /gene="nadR"
                     /locus_tag="WGR19_000905"
     CDS             complement(13414..13854)
                     /gene="nadR"
                     /locus_tag="WGR19_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0798457.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(14195..14704)
                     /locus_tag="WGR19_000906"
     CDS             complement(14195..14704)
                     /locus_tag="WGR19_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0798458.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14783..14858)
                     /locus_tag="WGR19_000907"
     tRNA            complement(14783..14858)
                     /locus_tag="WGR19_000907"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14823..14825),aa:Lys,seq:ttt)
     gene            15069..16148
                     /gene="apbC"
                     /locus_tag="WGR19_000908"
     CDS             15069..16148
                     /gene="apbC"
                     /locus_tag="WGR19_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0798459.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGGAHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            16929..17986
                     /locus_tag="WGR19_000909"
                     /pseudo
     CDS             16929..17986
                     /locus_tag="WGR19_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
BASE COUNT         4137 a         4927 c         4821 g         4101 t
ORIGIN      
        1 cactatagat tcccacttcc gtgggaatga cggttcagtt gcattccgac aacaccgtaa
       61 tcttgaaact cgtcattccc gacaacaccg caatctcgaa atccgtcatt cccacacagg
      121 cgggaatcca gacccctgac gcggcgggaa tctatcggaa atgactgaaa ccccgagatt
      181 ctagattccc actttcgtgg gaatgacggt tcagttgcgt tccgacaaca ccgcaatctc
      241 gaaatccgtc attcccgcac aggcgggaat ccagacccac gacgcggcgg gaatctatcg
      301 gaaatgactg aaacctcgag attctagatt cccactttcg tgggaatgac ggttcagttg
      361 cattccgaca acaccgcaat cttgaaaccc ctccgccgtt ataaagacaa atcgcggcac
      421 aaaaaatgcc gtctgaaatg ctgttcggcg gtttcagacg gcatttgctc aaactttatc
      481 aggcgtaatg gcgcgtttcg ccttctccgc cgacattctc tgcacagcgt ttgcagacgg
      541 tttcatggcc tgcaaccgcg cccacatcgc gggtgtagtg ccagcagcgt tcgcattttt
      601 caccatcact ggctttagcg gcaacggcaa gttcgctgcc tactttcact tctgctttag
      661 acaccagcaa agcaaagcgc aattcttcgc ccaaagcatt cagatagccg gccatttctt
      721 ccggcgcggt aatttcggct tcggcttgca aggacgaacc gacggttttg tcggcgcgca
      781 aaggctcgat ggcggcggtt accgcttcgc gggcttcgcg gattgccgtc cattttttca
      841 ccagttcggc ttcggctttt tcgttgatgg tcgggaactc gtgccaagta tggaaaagca
      901 cgctgtcttc tgcgccgccg ccgatgatgt cccacgcttc ttcgccggtg aagcacaaaa
      961 tcggtgcaat caagagaacc aggctgcgcg tgatgtggta cagggcggtt tgcgcgctgc
     1021 ggcgggcgcg gctgtcggct ttggtggtat agaggcggtc tttcaggata tcgaggtaga
     1081 acgcgcccaa gtcttccgag cagaaagaaa ccatttcttt cacggcaaaa tggaaggcat
     1141 aacgcggata gtaatcgcct gccagacgct cttgcagccg acgcgccaat accacggcgt
     1201 agcggtcgat ttccaccata tccgcctgtt gcacggcatc ttcaatagga ttgaagtcgc
     1261 tcaagttggc aaacaaaaag ctcaaggtat tgcggatacg gcggtagctt tcggttacgc
     1321 gcttgaggat ttctttggaa atcgccaatt cgccgctgta atcggtggat gccgcccaca
     1381 ggcgcaggat gtccgcgccg aactcgttat acacttcttg cggcgcgacg acgttgccga
     1441 tggatttcga cattttgcgc ccgttttgat ccaccacgaa accgtgggtc agaagctgtt
     1501 tgtacggcgc gcgacccatt gatgaggcac agccggtcag catagacgat tggaaccagc
     1561 cccggtgctg gtcgctgcct tcgagataca aatccgccgg ccattccaat tcttcgcgtt
     1621 gtttcaagac ggaataatgg gtcgagccgg agtcgaacca tacgtccatc gtgtcggaaa
     1681 gtttgtcgta atgttcgcaa tcttccgcgc tcaagagttc gctcttatcg agtgagaacc
     1741 acgcttcgat gcctttttct tcgatttttt gggcaacttt ttccaacaac tcggcagagt
     1801 tcggatgcag ttcaccggtt tctttgtgaa caaagaaagt catcggcgtg ccccaatagc
     1861 gttggcgcga aaccacccag tcgggacggc cttcaatcat cgcttccaaa cgcgcgcgcc
     1921 cccaagacgg gaaaaactcg gtatcgtcaa cggctttaat ggctttgtcg cgcaaggttt
     1981 taccgtcgtt tccggctttg tccataccga taaaccactg acctgtcgcg cggtaaatca
     2041 gcggcgtttt gtgccgccag cagtgcgcgt agctgtgttc gattttgccg tttgccaaga
     2101 ggttgccggt ttcttccagc cattgcagga tgacggggtt cgcctcccaa acacgcatac
     2161 cggcaacgcg cggcgtttcg ctaatgtatt tgccctcggc gttgacgggg ttgtaaagct
     2221 cgatgccgta tttgttgcag acggcgtagt cttccaaacc gtgcgcggga gcggtgtgca
     2281 ccaagccggt accggcatcg gtggtaacgt ggtcgccgtt gagcatagga atatcgcgtt
     2341 ccaaaaacgg atggttcatg tgcaggtttt ccagcttgtt gccggtagtt tcggcaagga
     2401 tggcgatgcc gtctgaaaag ccgtagcgtt tcaaagcgtc ttccgccaaa tctttcgcca
     2461 gcaccaattt gcctttgggc gtatcgatta attgatacac cacgtccgcc ccggccgaca
     2521 cggcttggct cgccggcagc gtccaaggcg tggtcgtcca gatgacggca aacgctttac
     2581 cttcgatacc tgccaaaccg aatgcggcgg caagcgcggc agtgtcttta aacggatagg
     2641 caacgtcaat cgcgggcgat actttgtctt tgtattccac ttccgcttcc gccagcgaag
     2701 aaccgcagtc caagcagaat tgaaccggtt tcgcgccccg gtagagatag ccggatttgt
     2761 agatttcacc gagcatacgc acggtatctg cttcggtttt gaaatccatg gtcaggtagg
     2821 gcttgtccca atcgcccaac acgcccagac ggataaagtc ttttttctgg cgggcaactt
     2881 gttcggcggc atattcgcgg cacaattcgc ggaaacgcgc tttgggcatg tctttgccgt
     2941 gcagcttttc taccatcact tcgataggca aaccgtgaca gtcccaaccc ggcacataag
     3001 gcgcgtcaaa accggcttgg gttttgctgc ggataatgat gtctttgaga attttgttga
     3061 cggcatgacc gatgtggatg tcgccgttgg catacggcgg gccgtcgtgc aggatgaact
     3121 tcggacggcc tttggcgatt tcgcgcagtt tttggtagcg tttttgctcg taccagcttt
     3181 tcagccatgc aggctcgcgc ttggcaagat tgccgcgcat cggaaacggg ctctcgagca
     3241 ggtttacggt tttactgtaa tcggtcattt tttaatctct attgttacaa taatttcggt
     3301 ttcagacggc attgcgcccc aaatagtatt tcacaacggg aaaaccctgt gccgtctgaa
     3361 cagttaaaag attgattgta gcccaatcgg atggtttgta taaggttttt ctaccaacac
     3421 tttgcggctt ccatatcggc ttcaatctgc cttttcagtt cttccatacc gtcaaacttt
     3481 tcctcatcgc gcagtttgtg caggaagcgg acgttcagcc cttgtccgta cagatcgcct
     3541 tgaaagtcga acaaatggac ttccagcttt tgaaaacgcc cgccgccgac ggtgggattg
     3601 aagccgaagc tcgccacgcc gcgccgcgtg ccgaatgcgc cgtccgcttc gacgacaaac
     3661 acgccgccga gggcataacg gtgaccgggc aggcggatgt tggcggtcgg ggcgtttaga
     3721 gtgcgcccga gttttctgcc gtgcaccacc ctgcccccca aaacgtagtc gtgtcccaaa
     3781 agttttttcg cataggcaag gttgccgtct gaaagggctt ggcggacggc ggtgctgctg
     3841 gtgcggatgt cttcgacgat gacggaaggc gtgcgctcgg tttgcatatc gggctgttgt
     3901 gccaaaagtt caaaacagcc ttcccgcccc gcaccaaaac ggaaatcgtc gccgacgagc
     3961 aaatagcgcg tattcaaagt ttgacgcagc aggcggtcga taaacccttg cgcggatatt
     4021 tcggaaaaat gccggtcgaa acgcaaaacc cagacggcat cgacgcagcc cgtgccttcc
     4081 agcaattcca gcttggtacg cagggggctg atgcggcacg gcggggtttt gccggtgcgg
     4141 cttgcaaaaa attctttagg ttgcggctcg aaaatgacgg caacgacggg caggccgcgc
     4201 gcatcggctt cgaggcggag tttttggagg atgtgtttgt gtccgaggtg tacgccgtcg
     4261 aaattgccta tggtaacggc ggccccgtgc ggaaagtcgg gcgcgttgtg ccgccccagc
     4321 ctgattctca ttgttatgtt cgggagtgtg gtcaaacagg cggtcattgt aaacggtatt
     4381 gcggtttata gacagtgtgc cgcccgttac gcccgcccga cgcgggaaaa gtaggcaaat
     4441 ttcccgcagc cgaacgcgcc aaacgcataa agcgcgcagc aggcgcggtg ctatgtgttg
     4501 aaacatcgcc ccaaacctga tgcaacatca gtgaaaatcg ttctttttta accaggtaaa
     4561 ccgagacaaa caaccctccg ccgtcattcc cgcgcaggcg ggaatccgga cctgtccgca
     4621 cagaaactta tcggataaaa acagttgctc aaaccccgag attctagatt cccactttcg
     4681 tgggaatgac ggttcagttg cgttccgaca acaccgtaat cttgaaactc gtcattcccg
     4741 ctcaggcggg aatctagaac gtggaatcta agaaaccgtt ttgcccgata agtttccgtg
     4801 cggacaggtc cggattcccg cctgcgcggg aatgacggca tttctgcggc aatcggatta
     4861 tttccaaacc aaaagcgcgt gattgcgttt gccgcgccga aggatggtgt atttgccgaa
     4921 acgtttgtgt tcgtcggtca gcaggcaggc atcgtcgggg cgttcggcgg cgtggttggg
     4981 gttgttggct tcggcaggtt tgccgttgag caaaaccgct ttgctgttca caaagccgcg
     5041 cgcttcttta ttggaggatg ccaaaccggt tttgaccaag gcttcgacga cattgatgcc
     5101 gtctgaaact tcaaatgcag gcaggccgtc gagggcgagc tgctcgaagt cgctttcggt
     5161 caggctgctt tggtcttcgg caaacaggct ttcggaaatg cgctgcgcgg cggcaagggc
     5221 ttcttcgccg tgaatcaggc gggtcatttc ttcggcgagg atgcgttgcg cttcgggctt
     5281 gctgccgctt gcctggtctt tggcttcgac ggcatcgatt tcttcgatgg acaggaaggt
     5341 aaagtatttc aggaatttat acacatcggc atcggcgact ttcagccaga attggtagaa
     5401 ctgatagggc gaggtttttt tcgcgttcag ccatactgcg ccgccttcgg ttttgccgaa
     5461 tttggtgccg tctgatttgg taaccaaagg cagggtcagg ccgaacactt gtttttgatt
     5521 caggcggcgg gtcaggtcga taccggcggt gatgttgccc cattggtcgg agccgccgat
     5581 ttccaaaacc gcgccgtggc gtttgttcaa ctcggcgaag tcgtagcctt gcagcaggga
     5641 gtaggcgaac tcggtgaagg aaatgcctgc gccgtcgcgg tcaatgcgct gtttgacgga
     5701 ttctttgttc agcatggcgt tgacggagaa atgcttgccg atgtcgcgca ggaagtcgag
     5761 gcagttcatt ttgccgaacc agtcggcgtt gttcgccata atggcggcgt ttccgccttc
     5821 aaagctcaag aaaggggtta attggctgcg gatgctttcc acccagccgg caacggtttc
     5881 ggcggaattc aagctgcgtt cggcggcttt gaagctgggg tcgccgatca taccggtcgc
     5941 accgcccacc agtgcaatcg gcgtatgccc cgcctgttgg aagcggcgca atgccaatac
     6001 gggcagcagg tgtccgatgt gcaggctgtc ggcggtcggg tcgaagccgc aataaaggga
     6061 aattttttgt tcgttcaaca aagcgtctaa ggcttcgatg tcggtggttt gcgcgataag
     6121 gccgcgcgat tgcaggtctt ggatgacgct catcggtctc tttcaaaaaa aattagcggt
     6181 tttgcaaacc gccgattgta acaaatttaa gcgaatcaat ggttatggcg cgtatcgaga
     6241 aaccgttgtt tttcggaaaa acgcttcgcc aattccgtgc cgccgtaagg gttgatgtgg
     6301 tctttgtccg agtaaaccgg caatccgccg atttggaaat ttgcggggat ataggcggcg
     6361 gcatcaataa tatagacgtt ggggtatttg gctgccaatt ccctgatgcg tgcattggct
     6421 ttcagagtgc tttcgtcgtc cgggcgcagg gtttggcggt aacccggtat gcgtgaagac
     6481 aagatatagg cgcgttgtac gttgtaagac gaggcaaggt tgtccgccat caggtaaacg
     6541 gcttgttttt cggatgagag tttgtgcagc atacggtcga atttttggaa aaaaccggca
     6601 tcgtaggcaa gggagcggct gttttcgggc atttggctgc cccagcgcat cgccaaaacc
     6661 acttttgaat acaggggcag gtgttcttcg gcatagcggt aaacggcgcg gcaggcggcc
     6721 cagttttgga acacacggga cgcgtagcct tccacatagg cgcaagcgtc ggcggaaacc
     6781 atagtggcgg accatttttc ttttttgccc acggcatcga agaatgtttt gtaatggtcg
     6841 gcgtgggaat cgcccaaaac cagcagttcc ggctgttttt ccgtatcccc ccataggcat
     6901 tgtttgccgg tattgttgtg gcaggaggtg ttggaacgcg tcagccccaa gcggtcgtat
     6961 tgcgccataa acggcagcct catcgcgaaa aacgagcccg cccccaaaat gagcataggc
     7021 aaggcataaa tccataaaac ggattgtgcg aacgaacctt tccatttttt aaacggcttt
     7081 tcgatgcagt gataagaaat cagggaaaac gctagagtca ggacgatcgc tgccgccggc
     7141 gaataaggcg gcaggttgtc cgggccgata tagcgcataa aggccaatat cggccaatgc
     7201 cacagataaa gcgaatagga aatcaaaccg gcggcaacgg tgattttcca ttggaaaaat
     7261 tttttaagcg ggtgttcgta atgattgaaa taaatcagcg cggcaacagc cagacaggga
     7321 atcaaagcgg cggggcccgg gaaataggcg gtttgttccg aataggaaaa caggcaggtt
     7381 gacaatatgc acacggcaaa caatgcgccg gcggcggcac agcgtctgcc gacggcaggt
     7441 tgccggcagc gcatccacac ggcggtcagc gatccgacca gcatttcgca ggcgcgcagg
     7501 tggggcaggt aatatttatc gaacgcggaa ggcataaagg aagcggcaag gcttaaggcg
     7561 cacaatgcgg cgaggaagcc gaactgtacg cgcgggcttt tgcgggcgac aagcagcagc
     7621 aatatcggaa agacaaagta aaattgttct tcgaccgaca aagaccaaat gtgcagcaag
     7681 ggcttttctt cctgcgcggg atcgaaataa tccttccccc ttgcaaaata caggttggag
     7741 gcgaaaccca aggcggtcag cgcggatttc cataaaagaa agaaatcatc tttggtgaat
     7801 aaaaagaagc cgcctgtcag cgttgccgcc aatacggcga aaaatgcggg cagaatccgc
     7861 ttgatgcggc ggatataaaa tgccttcagg gaaaacctcc cccccccccc gacatttcgc
     7921 ggtgaagaat catcgtcatc aaaaagcctg aaatcacaaa gaatatatcg acaccgagaa
     7981 acccgcccgg cagccaattt ttttcgatat ggaacacgat gacggacaag acggcggcgg
     8041 cgcgcaatgt gtcgatgtcc gggcggtagg gtaaggcttg gctcataata tttttgcttt
     8101 catcgcggcg gcgcaaatgc cgtctgaaag gtcattttgt atcggggctc gggatattcg
     8161 gaatgccgga cggctttttg caacagcggg gcaggcggtt tgttttgcag gaatcgggga
     8221 gggcaaatcg gaaatgcggg cgggatttta ttttgatgca gccgcattcc ggcggtatgg
     8281 aaacgccgaa agccaccaaa accgacttca gacggcattt tcggcaaagc cgtctgaaaa
     8341 cctgccgcat ttgggttaca cgttaaacaa aaagtgcatc acatccccat cctgcacgac
     8401 gtattcctta ccttccacac gcattttgcc ggcttctttg gctttggctt cgccgccgag
     8461 cgagacaaaa tcatcgtagg caatcacttg ggcgcggatg aagccgcgtt caaaatccgt
     8521 atgaatcacg ccggcggctt gcggggcggt gtcgcctttg tgtatcgtcc acgcgcggac
     8581 ttctttcacg ccggcggtga agtaggtttg caggcccaag aggtcgtagc cggcgcggat
     8641 caggcggttc aagccgggtt cttccaagcc catttcggcg aggaactcgg ctttttcgtc
     8701 gtcttccaat tcggcgattt cgctttccat cgcggcgcat acggcgacga caggggcgtt
     8761 ttcttttgca gccaattctt tcaggtggtc gaggtgcgga ttgttttcaa aaccgtcttc
     8821 ggaaacgttg ccgacgtaca tggcaggttt ggcagtcagc aagaacagcg gtttgagcat
     8881 ggcgcgttct tccgcgtcca aaccgaagga gcgcacgggt ttgccttcgt ccagatgcgg
     8941 cagcagtttt ttgcacaaat cgaccagctt ttgcgcgtct ttgtcgcctg agcgggcgcg
     9001 tttttcttcg cggacgatgg ctttttcgac acttgccagg tcggcaagtg ccaactctgt
     9061 gccgatggtt tcaatgtcgg caatcggatc gacgcggcct gcaacgtgga cgatgttgtc
     9121 gtcgtcaaag cagcgcacga cattcacaat cgcatcggtt tcgcggatgt tggcaaggaa
     9181 ctggttgccc aagccctcgc ctttgctcgc gcctgcaacc aaaccggcaa tatcgacaaa
     9241 ttcgacgatg gcaggctgca ttttttgcgg attgacgatt tttgccaatt cggccatacg
     9301 cggatcgggg acttcgacga tgccgacgtt gggttcgatg gtacagaaag gatagtttgc
     9361 cgcttcgata cccgattggg tcagcgcgtt aaaaagggtg gatttgccga cgttgggcaa
     9421 accgacgatg ccgcatttca aactcatgtt ttttcctgaa aatagagaaa tttaacggcg
     9481 gattatagca taccgccgcc cgcgttccga aaaaatgccg tctgaaacgg cttcagacgg
     9541 catccggttt caaaaaccgt tcagaacaag ccgtgaatca cgccgtgttc gtccacatcg
     9601 attttctcgg cggccggaac tttcggcaga cccggcatct tcatcatatt gccgcacagc
     9661 gcgacgatga agcccgcgcc ggcggaaacg gtgatgccgc gcacggcgat gcggaagtct
     9721 tcggggcagc ccaaaagttt ggcgttgtcg ctcaaagaat attgggtttt cgccatacag
     9781 accggcattt tgtccaaacc cagtttttcc agcgaggcga tttcggcaga cgcttccgcg
     9841 ctgaaatcga catcttccgc accgtacact ttttgggcaa tcgcacggat tttgtctttg
     9901 atgcccaact cgacatcgta ggcgaaaccg aagttattgg tttgactttc aatggcattg
     9961 acgactttgc gcgccaaatc cgcgccgccc gcaccacctt tgccccacac ttcggtcagg
    10021 gaaacttcaa cgccgtgttc cgcgcaggct ttttcaatca tggccaactc ggcatcggag
    10081 tcggacacaa agcggttgag cgcgacgacg acgggcagac cgaatacgtt tttcaggttg
    10141 gaaatgtgtt tcaagagatt aggcaggcct tttgccaaag cgtctaaatt ttcttcgccg
    10201 aggttggcgc gttccacgcc gccgttgtat ttcaaagcgc ggacagtcgc cacgacaaca
    10261 gccgcatcag gtttcaaacc ggcaaggcgg catttgatgt cgcagaattt ttccgcgccc
    10321 aagtccgcgc cgaagcctgc ttcggttacg gcgtaatcgg caaggtgttt cgccagacgg
    10381 gttgcggtta cggagttgca gccgtgggcg atgttggcga acgggccgcc gtgtacgaag
    10441 gcgggcgtgc cttcgatggt ttgcaccaag ttgggcttaa tcgcatcttt aagcaatgcc
    10501 gccatcgcgc cattcgcttt caaatctttg gcgtaaacgg ggctgccgtc tttggcgtag
    10561 gcgacaagga tgttgcccaa acgctctttc aaatcgctga tgtttttggc aagacagaat
    10621 accgccatca cttcggaagc aacggtaata tcaaaaccgt caggacgcat cacgccgtca
    10681 acaggtttac ccatgccgtc gatgatgttg cgcaactggc ggtcgttcat atcgaccacg
    10741 cgccgccaca gcacgcgttt ggggtcgatg tccaactcgt tgccctgata gatatggttg
    10801 tcgagcatgg cggcgagcag gttattcgcc gcgccgatgg catggaagtc gccggtaaag
    10861 tgcaggttga tgtcttccat cggcaaaact tgggcatagc cgccgcctgc cgcgccgcct
    10921 ttcacgccga acaccggacc caaagaaggc tcgcgcaggg cgataaccga gtctttacca
    10981 atatggcgca atgcatccgc caaaccgatg gttacagtgg ttttaccctc acccgcagga
    11041 gtcgggttga tggcggttac caaaatcagc ctgccttgtt tttgcggcag tttaaacgct
    11101 tcggcaggat tgattttggc tttgtaatga ccgtaaggct caatgttgtc ggcgttcaga
    11161 cccagcttgg cggcaatttc gccaatcggg cgcatcgtgg aagactgggc gatttcggca
    11221 tcggttttga agctcatgat tttcctttag aaatgaggag ggacatgccg tctgaaagca
    11281 tcaggcgaca aacaggtgga ttgaaaataa tatcaggcat attataacgt tatccgcacc
    11341 aaacccgcag tgaaattttt gacgcagcaa caaaaatacc gttcatattg ttcacaatcc
    11401 aaggagaaaa catgggcagc aacgtatggc tgttttgggc attggcatcg gcaggcttcg
    11461 cctcgctgac cgccattttc gccaaaatgg gtttgcaggg catcgattcg gatttcgcca
    11521 cctttatccg caccctggtg atcctcgccg ctttggtatt gtttttaacc tacaccggca
    11581 aatggcaggg tgtgaacggc tttacggggc gcaactggac attcctcatc ctatccggcc
    11641 ttgctaccgg cgcatcctgg ctcgcctact tcaaagctct gcaactgggc aacgcctcgc
    11701 aagtcgcgcc tgtcgacaaa ttcagcctgg tcttggtcgc gctgatggcg gtggttttct
    11761 tggacgaacg cccgaacacg caggaatgga taggcttggg gctggtaacc gcaggtgtgt
    11821 tgacgctggc actgaaacgt taaaccgaat ccgccatacc gcctgaaacc gggttttgac
    11881 aatctgacgg gcggcggtct gaacaaagtt acccaataaa atccgacacc gcgccgacgg
    11941 caaaccgccg catccgacag ggtcggcttc agatggcatt tgttggacgg aaagccccga
    12001 acgccctatt ttcacgcctc cacgaaaata tgctggacat ccggtcggaa ccgttcggca
    12061 gccgactcgg caagccaccg ttttatttca cttccaagcc cctgcaacgg cttgggcctc
    12121 tttcagacgg tctaccgtgc cgacatccag ccacaagccc gtatgcttct gtccgctcac
    12181 gcggttttgc cgcatttcgt tccgcaatac gggcgcgagt ttcgccacac tgcccgcttc
    12241 gattccgtca aacatttcag gacggtaaat gcccacgccg ctaaatgtca atccgttacc
    12301 gccggatact tccggctgca tgctgccgtc gggcagcagg gaaaaatcgc cgtcaggatg
    12361 gtgcggggga ttttccacca gccacagatg ggcggaaata tgttccggca gggacgatgc
    12421 cgtctgaaac gcggcggtaa aatcgatgtc ggtcaacacg tcgccgttga ccaccaaaaa
    12481 cggctgccca cccaacagcg gcaatgcctg cgcgataccg cccgccgttt ccaaaccgcc
    12541 tgcgggttcg ggcgaatagg cgatgttcac gccataagcc gagccgtcgc ccaaagcatc
    12601 ttctatctgc cgacccagcc aagcgtggtt gatgacgatt tcggtaaacc ccgcctgctt
    12661 cagacggcat aggtgccaac cgattagagg cttacccgcc acatcgagca gcggcttcgg
    12721 agtggtatcg gtcaaaggac gcatacgctc gccgcgtcct gccgccagta tcatcgcttt
    12781 catatatctg tccgaatatc agtctaaaaa tctaaactgc cgtctgaaat acagcagcgc
    12841 ggggcgtttg cacccgcagt ttttgatttc gtcgagcctg acgtaaaaca caaaatgcgt
    12901 gccgatttca tgtttgccga caatatgccc gtgcaggtgc gccaacgcgc cctctatttc
    12961 aagttgtccc gttttgccgc gatgccagat gtggtaggca aaccgctctt cgggcgacag
    13021 gccggtcagc ccggcaaaat gttcggcaac atcctgatgt tcgtccgcca gcgtattgat
    13081 gcagaggctg ccgttttccg acaggatcgg aatgatacgg gctttacggt tgatacacag
    13141 catcacggtc ggaggctcgt ccgtaaccgg tgtaaccgcc gtcattgtaa tgccgtaacg
    13201 ccctgccgca ccgtctgtcg tgatgacatg aacgcctgcc gcacaagatg ccatcgcatc
    13261 acggaacgaa gtttgaaaag ttttctgcaa atccgccatt tttccccttt aaactgtccc
    13321 ctatataaga atgctgcaca caaggcatcc cccatgtgca gcagttttga ttcaaaaagc
    13381 cgtcggtcgg acgtttccgc gcgttacggc gtattacgag ttcaacgcat cctcgatttt
    13441 ggcaagttct gccaacaggt ctttaagcag cagcattttc tcgcggccca gcacttcctc
    13501 gatagcgtcg taacgctcgt ccacttcttc gccgatttcc tcatacagct tctcgccctc
    13561 ggcagtcagc ttcagaaaaa cacggcgttg gtcgttggaa ggtttcaggc ggacaaccaa
    13621 acccgctttt tcaaggcggg tcaggatacc ggtcaggctg gggcgcaaaa tgcacgcctg
    13681 attcgccaaa tcttgaaagt ccagcgtgcc gttttccgcc aaaagacgga taatccgcca
    13741 ttgctgatcg gtaatattcg cctgattcag aataggcctg aattgggtca tcagggcttc
    13801 ccttgcctgt atcagaccga tattgataga cgcatgtttt gattgggtag gcattgttta
    13861 agtctccaag ttatcgaaaa tcaaactttc aaaccgtcgg gaaagcctgt gggcgtaaat
    13921 tttgatgcaa ccgttatata acaaaacgaa catatagcaa caatacgcta taaaccgcat
    13981 cggacgactg ggtataaaag actttaattc cgataatcct atctaaaaat attttaatag
    14041 ttatatctta atctattttt cccacaatca caacaaggga ttacatcggc aggcgcaccg
    14101 gctctttccc aaaaaacaaa agccgccgca tccgccgcgc aaggcatatg ccgtttgact
    14161 ctctacatag cggaaaattt aataaaaaca aaggttaacc gaaaacatcc gcctgaaaaa
    14221 ttcgtgcgcg caagccccaa taactgctga ttcccgtcgt atagtgaacc attttcccat
    14281 ttttgaccaa aacgacggca ggcgttgcga caatccgcca agaccttgcc aaacccccgt
    14341 cctcatcgtt gaccgtcgga aagcccaagc cccgctttgc catatacgcc gccacttcgg
    14401 acgcgctacc ggaacgtacc gccacgccga cgaccggcac gccgtccgcc gccaaatcat
    14461 cgattatcgg cgactgataa cggcacacgc cgcaccagct cccccaaaaa tacaccaaaa
    14521 ccgccttatc tcggctaaac tgtcccaaag tcagccgctg ccccgacagc agggtcaaag
    14581 gccgccctgc cgcaccggcc ggctcttcgg gcttgcgtat ccaatccaaa aacagcgaca
    14641 ccaataaaaa caccaatgcc gtctgaacgg caaatttgat gcccgaaagc agtttctttt
    14701 tcatacgctc tctcaaacgg tacgcccgcg caaccggcag gcaaacaaaa agccaagtct
    14761 caaaacttgg cttccggtta tctggtgggt cgtgagcgat tcgaacgctc gaccaacgga
    14821 ttaaaagtcc gctgctctac cgactgagct aacgacccga taagccgtgc attatacagc
    14881 accatcctgc ctcgtcaagc aaattttaca ggcttaattg cagaccactg tttgcacggg
    14941 atattttgac aacggatttt cacaatccgc cgcatatcgt gtaaaagttc gcacaaggaa
    15001 aggcaaaccg cccgaaatca atgtacactt tccgcccgtt tcccaaactg cacacagaaa
    15061 cacacattat gaacatacaa aacatccgca ccctcctcga caccgtcgcc gttccgaata
    15121 cggcacgcac gctcggcggc gaaaaggccg tccgttcggt cgaacagcgt tcagacggca
    15181 tccatatcgc cctgcatttc ggcttccccg tcgcgcacat cgccgccgcc gtcgccgatt
    15241 ccgtacaaga aatcctgatg cccgaaacag gcggcgcgca catccatctg tccatagaca
    15301 ccgaaatcgg cacacacaaa gtccagcccg gcgttaccac catcaaaggc gtgaaaaaca
    15361 tcatcgccgt cgcctctggc aaaggcggcg tgggcaaatc aacaaccacc gccaaccttg
    15421 ccgccgcaat ggcgcgcatg ggcgcgcgcg tcggcgtgct cgatgccgac ctttacggcc
    15481 cgagccaacc gaccatgttg ggtgtggacg accgcaaacc cgatcagaaa aaccaaaaac
    15541 tcattcccgt cgaatcttca gacggcatac aggtcatgtc tatcggcttt ctcgtcgata
    15601 ccgaccaagc cgtcgtctgg cgcgggccga tggtcagcca agccttgcag caactgatgt
    15661 ttcaaagcga gtgggacgaa gtggattatc ttttcatcga cctgcccccc ggtaccggcg
    15721 acatccaact cacactgtcc caacgcatcc ccgttaccgg gtccgtcatc gtaaccacgc
    15781 cgcaggacat cgcgctgata gacgcgcgca aggctgtgga tatgttccgc aaggtcaaca
    15841 ttcccatttt aggcgtattg gaaaacatgt ccgtccacat ctgctccaac tgcgggcata
    15901 gcgaagcact gttcggcacg gacggcggca aagatttggc ggcccgcctc aacgtccccc
    15961 tgctcggaca gcttccccta agcctgcccg tgcgcgaagc catggacggc ggcacaccgg
    16021 cgcaactgtt cgacgaacac cccgccatcg cccgaatcta caccgatgcc gcattccaaa
    16081 tcgccctggg cattgccgac aaaggcaaag acttcagcag ccggttccct aaaattgtcg
    16141 tcgaataaag ccgcgtccga aaccgcaaca gcaatgccgt cccaagcccc gcgcctgccg
    16201 gcgggcaaac ttgccgggta aacggtttct tgagattccg cgtcctggat tcccactttc
    16261 gtgggaatga cgggatttta ggtttctgtt tttggttttc tgtttttgta ggaatgatga
    16321 aattttgagt tttaggaatt tattggaaaa aacagaaacc gctctaccgt cattcccgcg
    16381 caggcgggaa tccagacctt agaataacag caatattcaa agattatctg aaagtccgag
    16441 attctagatt cccactttcg tgggaatgac gcggtgcagg tttccgtatg gatgggttcg
    16501 tcattcccgc gcaggcggga atccagaccg ttgggcatct gcagcggttt gctaaaaacc
    16561 gctttactgt gataagtgcg cagggttaga atggcgcggt aaccttatat attgtacccc
    16621 gtcaaagggg cgcattgctt ttcttaacat tcccctttag cagccaagtg aaagggcttt
    16681 tcaatcagca atccggcggg cgcgggatcg ggcggtttgc cgaatcccgc cgtttgcggc
    16741 gcgcctgccg ccgacggtat cccgcgaagc aagatttaag ggataaaata tgttccaaca
    16801 cgcagggcgg cacataaggc gccgccctga ttcggaaggg cttgcacccc tcccggacaa
    16861 agcctgatcc tgccgccccg aaggacggat gcccgaaggg cggggggttt gaccgaaaag
    16921 gaaatacgat gaataaaact ttaaaaaggc aggttttccg ccataccgcg ctttatgccg
    16981 ccatcttgat gttttcccat accggcgggg ggggggggcg caggcggaaa cccataacta
    17041 cgctattgtc atgaacgaac gaaatcagct ggaggtaaag caggagggtt catattcaac
    17101 cttaagggaa aaagacagga aacgcaaatt tatctataat aaagtcggac agggtggagg
    17161 ctctgtcttt ttcgacaata ccgataccct tgtttcccaa caaagaggta ctgccgtttt
    17221 tggcacagcc acctacctgc cgccctacgg caaggtttcc ggttttgatg acaaaaggct
    17281 gaaagagcgc ggcaatgccg ttaattggat tcatacgacc cacccagggt tgataggcta
    17341 cagctacgcc ggtgtcgtat gcagaagcat ccaatgcccc caacttgtct ataaaacccg
    17401 attttccttc gacaacaccg gtttggcaaa aaatgcgggc aggctgaata ggcacccgga
    17461 cccaagccgc gaaaattcgc ccatttacaa attgaaagat catccatggt tgggcgtgtc
    17521 tttcaatttg ggtaccgcca aagatggtaa gacaatcaac aaattggtat cttcttttga
    17581 tgaaaagaat agtaataaca acctcgtcta taccacggaa ggccgcggta tttcccttgg
    17641 cgactggcag cgcgaacata ccgccgtggc ctattatctg aacgccaaac tgcacctgct
    17701 ggataaaaaa cagattcaaa atatcaccgg caaaacagtg cggttgggtg tcttgaagcc
    17761 gagcatcgat gtgaagacac aaaatacggg gcttggcggc attctatttt ttcatgctaa
    17821 gtgggacatt aaagataacg ggcagattcc ggtcaagctc gacctgtcgc aagtcaaagc
    17881 aggccgctgc atcaacaaac cgaaccccaa taataatacc aaagtccctt cgcccgccct
    17941 gactgccccc gcgctgtggt tcggacctgt gcaaaatggt aaggcg
//
LOCUS       DAWFJW010000033        17839 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFJW010000033 DAWFJW010000000
VERSION     DAWFJW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17839
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.033"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(181..492)
                     /locus_tag="WGR19_000910"
     CDS             complement(181..492)
                     /locus_tag="WGR19_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798460.1"
                     /translation="MEYLTTTIPTHFEIERTRLADVILTKLSDDKAVKLAKQMLDEHE
                     YIESLLVNTDPSVDDVKELANALYDHIRFEERELFPIAETVLSDDELFAIYEASDENV
                     K"
     gene            complement(1011..1703)
                     /locus_tag="WGR19_000911"
     CDS             complement(1011..1703)
                     /locus_tag="WGR19_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ0798461.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(1771..3747)
                     /locus_tag="WGR19_000912"
     CDS             complement(1771..3747)
                     /locus_tag="WGR19_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0798462.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIAAPQSFGMNISDIDNKPYFQAIEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRTRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            3925..4590
                     /locus_tag="WGR19_000913"
     CDS             3925..4590
                     /locus_tag="WGR19_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0798463.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            4642..5469
                     /gene="mutM"
                     /locus_tag="WGR19_000914"
     CDS             4642..5469
                     /gene="mutM"
                     /locus_tag="WGR19_000914"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0798464.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQVNPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGKPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEVFCADYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            5557..6324
                     /locus_tag="WGR19_000915"
     CDS             5557..6324
                     /locus_tag="WGR19_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0798465.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(6635..6946)
                     /locus_tag="WGR19_000916"
     CDS             complement(6635..6946)
                     /locus_tag="WGR19_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0798466.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            7475..9754
                     /gene="nrdA"
                     /locus_tag="WGR19_000917"
     CDS             7475..9754
                     /gene="nrdA"
                     /locus_tag="WGR19_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0798467.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            9775..10347
                     /locus_tag="WGR19_000918"
     CDS             9775..10347
                     /locus_tag="WGR19_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798468.1"
                     /translation="MKAFPKFDYQGIEYDLTHLTAFSCDYIQPAKSDGTAEKRYRCII
                     EFSTHCFTRGENKRKGETLSDIEPALQYITAKETRIFCFERYQVSKMLPQIMREISRN
                     KCYFTSADDKFLTISVTDKNGKKVDYEIYFSLQRAKSPKHDVHIYINSAYIRDGDYKE
                     NHGTKVRRKPVGFFVLLHNTLVNKRIKRPK"
     gene            10619..11752
                     /gene="nrdB"
                     /locus_tag="WGR19_000919"
     CDS             10619..11752
                     /gene="nrdB"
                     /locus_tag="WGR19_000919"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0798469.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            11873..12163
                     /locus_tag="WGR19_000920"
     CDS             11873..12163
                     /locus_tag="WGR19_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0798470.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCYVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(12204..12482)
                     /gene="ftsB"
                     /locus_tag="WGR19_000921"
     CDS             complement(12204..12482)
                     /gene="ftsB"
                     /locus_tag="WGR19_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0798471.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(12496..13782)
                     /gene="eno"
                     /locus_tag="WGR19_000922"
     CDS             complement(12496..13782)
                     /gene="eno"
                     /locus_tag="WGR19_000922"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0798472.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            complement(13829..14269)
                     /locus_tag="WGR19_000923"
     CDS             complement(13829..14269)
                     /locus_tag="WGR19_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0798473.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKEVV"
     gene            complement(14284..15126)
                     /gene="kdsA"
                     /locus_tag="WGR19_000924"
     CDS             complement(14284..15126)
                     /gene="kdsA"
                     /locus_tag="WGR19_000924"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0798474.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LDIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVTTMAKTGKVVNIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(15148..15531)
                     /locus_tag="WGR19_000925"
     CDS             complement(15148..15531)
                     /locus_tag="WGR19_000925"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0798475.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(15680..>17839)
                     /gene="mfd"
                     /locus_tag="WGR19_000926"
     CDS             complement(15680..>17839)
                     /gene="mfd"
                     /locus_tag="WGR19_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0798476.1"
                     /translation="GEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLL
                     RDLAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLI
                     SRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINE
                     LDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVA
                     VMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGT
                     VDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPR
                     TLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEV
                     DTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDI
                     PNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAAD
                     ELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLD
                     APLGITTEIKLHSPTLLPEDYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLP
                     EQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYR
                     LAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
BASE COUNT         4061 a         4069 c         5050 g         4659 t
ORIGIN      
        1 tattataata ttaaagctac tttgtggcgg acagcgtgga gcatagcacc gataaacctg
       61 tgtttaatca gattgtggga ttaaaagatg ggtttaaata acttttagca attttaaaat
      121 taaacgaaag ccaaacgtgg tgtttggctt tttaattcca ttgcccagct tttaactcgt
      181 tcattttaca ttctcatcac tcgcctcata aatggcaaaa agctcatcat cgcttagcac
      241 cgtctcagca atgggaaaaa gctctcgctc ttcaaagcgg atatggtcat acagagcatt
      301 tgcaagctcc ttgacatcgt cgacgcttgg gtcggtattg acaagtaggc tctcaatgta
      361 ttcatgctca tcaagcattt gctttgccaa tttaaccgct ttgtcatcgg ataattttgt
      421 caaaataaca tcggcaaggc gtgttcgctc aatctcaaaa tgcgtgggaa tggtagtggt
      481 cagatactcc acttaggtta atcggctgga tcaggtttga cgattggtgt tggaagacat
      541 attgcaacag tgtttgatta agatgagatg gaaattctac acgcgttttt taacaggaac
      601 tgactgattg ttttttaagt gtgtagttat tttcactaat agagttatat tttatagtgg
      661 atatttctga tttgcataaa tcaacaagga ttgaatcaga ttttatagat agattaattg
      721 tttatttttc tacaacggga cggtgaaatg aaatttcata tatcatatgg gcgaagatgt
      781 tgcaccagta atatatgtag taaatgaaga taaaaaaaac catctataca aggggaagta
      841 tagatggcca aacacattac ggggaaaacg tcttactcat aagcctgctt gaacaggcgt
      901 tactcagaca aatggattat atcgtaaatt gattttttgc gttaaattgg gttaaaccat
      961 atatttaatg agtatgtgga tgaatataaa tggaattctt ggatgttatt tcagaatttt
     1021 ggacattcga taaatttcca ttccccgggt ttgaggtttt ctgtttccag atgtgcgaat
     1081 cgccggcgat gaaggtgttg cacgcggttg ccggcggcgg cgatcatgcg tttgacttgg
     1141 tggtatttcc cttcggtaat ggtcagcagc agggtggtcg ggttttccaa aacggcatcg
     1201 gcggcacaaa cggtttcgtt ttcgtcgtgg agcagcacgc cgtttttcaa ggtttcgcag
     1261 agcgtttctc ctgtggggtg tttgagcgtt acttcgtata gcttgggaat ttttctgctc
     1321 ggcgaagtca ggttgtggtt cagtttgccg tcgttggtaa tcagcaatac gccggtcgta
     1381 tccgcatcca gcctgccgac cgcctgcata tcgatgttcc gcatattgtc ggggaacagg
     1441 ctgaatacgc tgcggtagtg cttgggtttg tgcgaagttt cgtaatcttc aggcttgttg
     1501 agcatgatgt agaaataggg ttcgggaacg acggttactg cttccccgtc aatatccaac
     1561 gtttcgacgg atgaggaatc gatgtctgca tcggtgtcgt ccatgcaggt tccgttgatg
     1621 aaaacataac cgccggcaat cagccattgg cactgcttgc ggcttcctat gccttgatat
     1681 tgcaggtatt tgataagttt catgatggta tgggaaatgt gggatgaaaa agacgggact
     1741 gagaaagtcc tgtcatgtgc cgtatccgaa tcagcttcca atcagtttga ccctctgtcc
     1801 cggattgatg gtgttcaggt tgggattgag ccggcggatg tcgtcgatat ggatattgaa
     1861 gcgcgcggcg atacttttga aggtgtcgcc tttgcgcgcg gtgtaggata ctttttcaat
     1921 acgggtttgg gcaggggctg cctgcgccag gcggagtacc tgtccttttt ggatggtgtt
     1981 gcctttgatg ttgttggcga caatcaggtc ggctacactg acgttgtagc gtttggcaat
     2041 gttgaacagg gtgtcgcctt ctacaacgcg gtggatgctg gcatggagcg gggagatttc
     2101 ggcttcgccg tcggcagctt tggctacgcg cgtttccaaa cgtgtccggc gtttgccgtc
     2161 ggcggcaacg gtatgttgcg gagatgagga aatgttgtgt tcggcaactg tgtcaggcgt
     2221 gccgatgacg gcggagatgg tttcttcagc ctgtcggcgc aggttgtttc gggcaacaag
     2281 ctgcatgagt tcgtctgccg cctgcatgtc gttttggggg acgatctgcg cgagtgttgc
     2341 ggtttgggtt tcagacggca tggcggtctg tttttcggtt tgaggttgcg ctgcggctgt
     2401 cgcaagggca ggttcggcaa tacggacaag gggatcgggt tccgtacgga cggttttctg
     2461 cggcaaaggt gcgacggtaa tgtccgctgt ctgtgcggcg gcgggtcgga ttcgggcaat
     2521 gccgacgttc accgtgcctg ccggcatatt ggaacggtag gtgtcgggcg tattgtcgat
     2581 gtcgatggaa acgacggatt ccgatgccgt atgaagggtt ttgccgttct tggcgacaag
     2641 gatgctgcgt cctgcgttga caaggttgcc gttcaggttg ttgaggcgtt tgatgtcggc
     2701 aatgctcatg ccggttgccg tcgagatgtc ggacaggctg gttttggcgg caggtgtata
     2761 gacttcccat gaaaacaggc tgtcgggtgc ggcgttgagg tagttgcttt ggaaggtttg
     2821 gacggatgcg acaggaagca gcagtttgcg tttgcttttg gggatgaacg cggggacgtt
     2881 gaatgcgggg tttagggcga gcagctcgct ttgcgtgata ccggcaagcc gggcgatggc
     2941 ttcgttgtcg agcggacggt ccggttcgat tgcctgaaaa tacggtttgt tgtctatgtc
     3001 gctgatattc atgccgaaag attggggggc ggcaatgatg ttgcgcacgg cgagcagctt
     3061 gggaacatag ttgcgcgttt cgttgggcat acgcaggttt tcgtaggtcg gttcgagccc
     3121 ttgggcgcgg gcgcggttga tggcgcgtcc gacgttgcct tcaccccagt tgtaggcggc
     3181 aaaggcgagc ggccagtcgc cgaacagtcc atagaggtat tgcagatagt tgagtgcggc
     3241 atcggtggcg gcgtaaatgt cgtgcctgcc gtcgtaaacc ggtgtttttt ccaggccgta
     3301 atgcctgccg gtagcgggca tgaactgcca caggcccgat gcgccgacgt gtgatttggc
     3361 tttggtgacg aacgcgcttt cgatgaaggg aagcagggcg gcttcggcgg gcatattgcg
     3421 ttttttgact tcgttggcga tatggtacat atagggtcta ctccggttga tgaccctgtt
     3481 gaaatagctg tggcttgcga tgaatttgct ttcgtggcgg cgtaccagtt cgggattgac
     3541 ttcgcccatc cggaagcctt ggcgcagctc gctccacagg ctgccggatt ggaaatattg
     3601 ttttgtcggt ggcaggtcga gtattgaaga gtttaagcgc ataatcgcca aaccgatttg
     3661 gtgtgatgag gtgttttggg cgtataggaa acccggacaa acggacagac ctgacgcggt
     3721 cagggcgatg gttttgagtt tggacatgat ttatatcggc ggaataaaca tattttatcg
     3781 tccgatggta ctgtttgact gaaaaagcgt caagttttac aggaacttta tgcggattcg
     3841 gaaatgggcg gccgatgtgt tttttgacag aaatcctgtt tggaatagaa tatcggcagg
     3901 caatctgttt gaaacggacg caatatggat gcatggtttg aagatacggc gatggggcgg
     3961 tatgttgcaa aattggaaca ggatttcttc gggcggtatc tggattcata ccgtttttcg
     4021 ggaatgtgtg cggttcaggt gggcggtccg tggctgagcc tgtctgaaga tgttgtctgt
     4081 gtgccgcgag atatgtcgat gtcggcggag aatatggctt tggcggatgt ttctgcggat
     4141 atgctgcttt ttccgcatac gcttgaaggc ggtatccctt cgcaaatcct gtccgaggcg
     4201 caccggatat tgaaaccgtg cggacgcttg atgctgacgg gtttcaatcc gtattcgctc
     4261 tggggattca gccgttggtt tgacggcgaa cgcctgccgg aaaaacggtt ttgtctgccg
     4321 ctgcccgagt tgaaaaggca gcttgcggat gtcggtttcg atattgaatt tgggaaattt
     4381 atggtgtatc tgccgcccgt ttcgtcgatc gggcaaatac gcttttggcg gtttatggaa
     4441 aaggcgggcg accgttggtg gccgcaatgt gccgcagtgt acgctttggt tttggtcaaa
     4501 agggcggcgg gcgtaacgcc cctgcccgcg tgggacgggt atttgggcgg caaagccctt
     4561 gcggcgggtg cggcaagggt tgcggattag ggacggccgt ctttcagacg gcatctgtca
     4621 gaaaaaatgt cgggaggggt tatgccggaa ttgccggaag tggaaacgac gttgcgcggc
     4681 atcgcgccgc atattgaagg aaaaacggtg gaagccgtgg tattgcgcca attgaagctg
     4741 cgctggcagg tcaatcccga tttgggggag attttgtccg gcaggcaggt tttgtcttgc
     4801 ggcaggcggg cgaaatatct gattgtccgt tttcaaacag ggattttgct gattcacttg
     4861 gggatgtcgg gcagtttgcg gatttttacg ccgtcggacg gacgtatcgg caagccggac
     4921 agacacgatc acgtcgatat tgtgttttca gacggcacgg tcatgcgtta ccgcgatccg
     4981 agaaagttcg gcgcgatact ttggtatgag ggaatcgaag aacatcatcc gctgttggaa
     5041 aaactggggc ctgaacctct gtcggaagta ttttgtgcgg attatctgta tgtgaggctg
     5101 aaggcgcaga aacgcgcggt caaacttgcc ctgatggaca atgcggtcgt ggtcggtgtg
     5161 ggcaacattt atgccaacga gagcctgttc agagcgggca tttcgcccca ccgtcctgcc
     5221 aaccgtctga aaaagaaaga gtgcgcgctt ttggttgaaa ccgtcaaagc ggtgttgcgg
     5281 cgcgccattg aaacgggcgg cagtaccttg agggattttg tggacagcga cggcaaaagc
     5341 ggctacttcc aacaggaata tacagtgtac gggcggcaca atcagccgtg cccccagtgc
     5401 ggcggtttgg ttgtgaaaga aactttgggg cagcgcggca cgttttattg cccgaactgt
     5461 cagaaatagg actgaaaacg gtttcagacg gcattttatc ggtatgccgt ccgaacgttt
     5521 caacaacaaa caccgattat cgggaaagaa ttgctcatgt cttcaaataa agcttcattt
     5581 tttacacgtc tgcgccgctt gtgccgtttg gcggtctggc tgttcaaaac cgggaaaaac
     5641 ctgcgcggta ttgacggcgg ttgccccgag tcgcgcaatc gggcggtaat cgagttgggc
     5701 aggggggttt tggcggcttt ggatatcgga ttggaggtgg gcagacccgc acccgaacat
     5761 ccgaacggtg tcttggttgc cgccaaccac gtgtcctggc tggatatttt cgcgatgagc
     5821 gcggtttatc cgagcagctt tatcgccaag caggaaatca aaagctggcc ggtattgggc
     5881 aagatggggc agaacgcggg aacggtgttc atcaaccgca attcgcggcg cgacatcgaa
     5941 ccgattaacc gcgccgtctg cgaaaccttg caacgcggtc aaaacgtcag ttttttcccc
     6001 gaagcgcgga cttcctccgg attggggctt ttgccgttca aagccgcgct gttccaatcc
     6061 gccatcgatg cgggggcaaa ggttttggcg gtcgcgctgc gttattatga cgaaacggga
     6121 aaaaggacgg ctcgtccctc atatgccgat gtcggtttgc cgacctgcct gtggcgcatc
     6181 gtgtctatga aaaaattgac gataaaagtc gatttcgttt gcgtggcgga tgcggcggaa
     6241 agcgaagacc gttatgcttt aaaagataaa atcgaagaaa gcatccgtgc cgttgtcgcc
     6301 gacgatgcgg atatcgctgt ctgaaaccgg ttgtcggaat gtggcagtat gattcgcttt
     6361 tgtggatgta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga
     6421 gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt ttcgtactat ctgtactgtc
     6481 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atattaaggc tgcggcaacg
     6541 ggaggacctc aaacgaaatg gcgtaaggat agtttgttgt atctatggaa aatgccgtct
     6601 gaacctgtgt tcagacggca tattttatat gggattaacg gcggaaactg ccgccgtcga
     6661 aatcatcgaa agcctcataa gccgcccggc attggatttt ctgttccgca taaggcaggg
     6721 agcggaatgc tttgcgcgcc tttttgtttt cgcgcaattc ctgcacgttg ccttgcgatc
     6781 ggtaccacgc ggcacggcgt tccaaatatt tcctgcattc ggggtgcagc atctgcttgt
     6841 ttgccttgcg gatatgtttg gtatcgcggt gggcgggttc tgacgtaccg gcagcggcag
     6901 ttgtcgatac cagtcccaat attgcagaca acagtatggc aagcatcttt ttgacagaca
     6961 tgacgatcct ttcagtttgt cattccttcc agtataggat ggaaggatgg aacgggcaaa
     7021 ttgtcggcag tttcagactc attcagccga caaaacttgc cgtttgatat gcggattcaa
     7081 ataacacatt aatctcaata ataaatttca atcggtaaga aaatggaatt tgtcggcggc
     7141 ggcgcggcaa aatcatactt tgcaaaattt aacaattcgc aggggcagaa aaccggaagc
     7201 tttccttttt cgtcggaaaa tccttatttc accgccttgt agccggagcc ggtcaaaagg
     7261 caaaaaattt acccgttttt tatcggtaaa gaattatcag ataaaacaaa tattatagga
     7321 aaaatacgac aggcgggttt tatcgcgcat tgcctgaaac tgaaaaatgc aaccgttgtc
     7381 aagactggag aaaatgccaa aaatccacta tattgtctgt cttaatttat ttgaaaagac
     7441 cgtgtcttga atatcaagag tggaagagga agcgatgaat acaccgactg atttgaaagt
     7501 aaccaaacga gacggaagat tagaagccat tgatttggat aagattcacc gtgtcgtcac
     7561 ttgggcggcg gacggattgg aaaatgtttc cgtgtcgcag gtcgagttga aatcgcacat
     7621 ccagttctac aacggcatcc gcaccgacga catccacgaa accatcatca aagccgctgc
     7681 cgatttaatt tcggaagata ccccggacta ccaatacctt gccgcgcggt tggccatttt
     7741 ccatctgcgt aaaatagcct acggcgaata cgagccgccg cacctttacg accacgttaa
     7801 aaaacttacc gatgctggaa aatacgacag gcatatcctt gaggattaca gccgcgaaga
     7861 atttgacgag ctgaacgcct atatcgacca cgaacgcgat atgtcctttt cctatgccgc
     7921 tgtcaaacag ctcgaaggca aatatctggt acagaaccgc gttacccgcc agatttatga
     7981 aacaccgcag ttcttatatg ttctggtggc gatgtgtctt ttcagcaaat atcccaaaga
     8041 gacacgcctc gattacgtca aacggtttta cgatgccgtt tccacattta aagtatcgct
     8101 gccgaccccg attatgagcg gcgtgcgtac gcctacgcgc cagttctcaa gctgcgtatt
     8161 gatcgaatgc gacgacagcc tggattccat caacgccacc accagcgcga ttgtgaaata
     8221 cgtttcccag cgtgcgggca tcggcatcaa tgccggacgt atccgcggac tggacagcga
     8281 aatccggggc ggcgaagcac ggcataccgg ctgcattccc ttctttaaaa tgtttcaggc
     8341 ggcagtcaaa tcctgttcgc aaggtggcgt gcgcggcggc gcggcaacct tgttctaccc
     8401 cttgtggcat atcgaagccg aaagcctgct ggtgttgaaa aacaaccggg gcgtggaaga
     8461 caaccgtatc cgtcagcttg attacggcgt gcaaatcaac cgcctgctgt acacccgcct
     8521 gattaagggc ggcaacatta cgctgttttc gcccaacgag gttccgggat tgtacgaagc
     8581 gttttttgcc gaccaagacg aatttgagcg gctctatacg aaatacgagc aagaccctga
     8641 tatccgcaag cgcatcattc cggctgccga cctgttttcc acgctgatgc aggagcgtgc
     8701 cggaaccggg cgcatctaca ttcaaaacgt cgatcactgc aatacgcaca gcccgttcga
     8761 tccgcgcgtt gcgcctgttc atcagtccaa cttgtgtatg gaaatcgcgc tgccgaccaa
     8821 acctttggac aatatcaacg acccgaacgg cgaaatcgcc ctgtgtacac tgtctgcctt
     8881 taacttgggc gcattaaaca gcttggacga attggaaggg cttgccgatt tgaccgtgcg
     8941 cgcgctcgat gcacttttag attatcaggg atatccggta gaagccgcgc gtacctccac
     9001 tatgggccgc cgttcgctcg gtatcggcgt gattaactat gcgtattatt tggcgaaaaa
     9061 cggtgtccgc tacagcgacg gctccgcgct cggtctgacc caccgtacct ttgaagccat
     9121 acagtattac ctgctcaaag catcggcaaa ccttgccaaa gaatacggtg cgtgcacgct
     9181 ctttaaccaa accgtttatt cgcaaggcaa actgcccatc gacacttaca aaaaagattt
     9241 ggatgccgtc tgcggcgagc ctttgcatta cgactgggaa agcctgcgtg ccgacatcgt
     9301 caaatacggc ctgcgtaact ctactctaac cgcactcatg ccgtctgaaa ccagttcgca
     9361 aatcgccaac gccaccaacg gtatcgagcc gccgcgcgga ttggtaacgg tcaaagcatc
     9421 gaaagacggc attttgaaac aagtcgtgcc ggagtttgaa accctgaaaa atgcctatga
     9481 aaccctgtgg cagcttcccg gcaacgaagg ctatctgaaa cttgttggcg tgatgcaaaa
     9541 attcgtcgat caggcgattt ccgccaacac cgcctacgac ccgggcaaat ttgaaggcgg
     9601 caaagtttct atgaaacaaa tgctcaaaga tctgctgacc gcctacaaat atggcgtcaa
     9661 aaccctgtac taccacaaca cccgtgacgg cgcggacgac acgcagaccg atattcagga
     9721 tgacggctgc gcgggtgggg cttgtaagat ttgataaggg ctttggtgaa gtaattgaaa
     9781 gcctttccta aatttgatta tcaaggtatt gaatatgatc taacgcattt aactgcgttt
     9841 tcttgtgact atattcaacc tgctaaaagt gatggtacgg cggaaaaacg ataccgctgt
     9901 attatcgagt ttagtacaca ttgttttaca agaggtgaaa ataaacgtaa gggagaaacg
     9961 ttatcagata ttgaacctgc attgcagtat ataacggcta aagaaacacg aattttttgt
    10021 ttcgaacgtt atcaggtttc aaaaatgttg ccgcaaatta tgcgtgaaat tagtagaaac
    10081 aaatgttatt tcacttcggc tgatgataag tttctgacca tatcagtaac ggataaaaac
    10141 ggtaaaaaag tagattatga aatttacttt tcacttcaaa gagccaaatc tccaaaacat
    10201 gatgtacata tttacattaa tagtgcatat atacgggatg gggattacaa agaaaatcac
    10261 ggaacaaaag taaggagaaa accggtcggt ttctttgtgt tattacataa tacattggtc
    10321 aataagagaa tcaaacgccc aaagtgaaac aaccctcact taaaagcaag ggttgtcagt
    10381 acaggcttcg ctttataact gctcttgggc tagtagccta aaaaggtcgg acaaacgctg
    10441 aaatggtttc atgcctgctc atgcgttttg ttgagacaaa ttgtaccacg attgcaataa
    10501 ggctcaaaat aaaaattttc agacgaccca tatgctttgc atagcctaga agtcgcctat
    10561 cctactttta gatttaaatc aacctgagaa aacagctatg tcctgcgaac acctagtcat
    10621 gtcatacagc acctttccca aaaccaaaaa cgacgcgctg aatgagccga tgtttttcgg
    10681 tcagcctgtg aatgtcgccc gttatgacca gcagaaatac gaggtatttg aaaaactgat
    10741 tgaaaaacaa ttgtcgtttt tttggcggcc ggaagaaatc gacgtgtcgc gcgaccgtat
    10801 cgactacgcc aacctgccgg aacacgaaaa acatattttc atcagcaatc tgaaatacca
    10861 aaccctgctc gattcgattc aaggccgcag tccgaatgtt gccttgctgc ctttggtgtc
    10921 gattcccgag ctggaaacgt ggattgaaac gtggagcttc agcgaaacca tccactcgcg
    10981 cagctatacc cacatcatcc gcaatattgt gaatgatccg tcggtcgtgt ttgatgatat
    11041 tgtcgaaaac gaatacatta ccgcccgcgc cgaagacatt gcctgctatt acgacgactt
    11101 aatcgaatac acccagtatt acaatctgtt gggcgaaggg gtgcacaatg tcggcggaaa
    11161 acccattact gtgtctttgc gcgggttgaa gaaaaaactc tatctctgcc tgatgtgcgt
    11221 caacgtgttg gaagctatcc gtttctacgt ttcattcgcc tgctcgtttg cttttgccga
    11281 gcgcgagttg atggaaggca acgccaaaat catcaaactg attgcccgcg acgaagccct
    11341 gcacctgacc ggcacgcagc atatgcttaa tctgatgcgt tctggtgttg atgattctga
    11401 aatggcggaa attgccgccg agttgcagga cgaatgtttc caactcttca aaaaagcggc
    11461 ggaacaggaa aaagaatggg cggcatattt gtttaaggac ggttcgatga tcggtttgaa
    11521 caaagaaatc ttatcccaat acgtcgaata tattaccaat ctgcgtatgc aggcggtggg
    11581 gttgccggcc ggatttgaag gcgcaaatca aaacccgatt ccgtggatta atgcgtggct
    11641 gtcgtccgac aacgtacagg tcgcgccgca ggaagtggaa atatcctctt atttgatcgg
    11701 tcagatcgat tctgaagtga atacggatga tttgggcgat tttgagttgt aaacgttttt
    11761 tgacaaaatg ccgtctgaag ccggatgttt cagacggcat ttgtatgttg tatgatggga
    11821 aaaacagcgg gtaggatcgg ttattgttcc ttttattttt gattggtcgg aaatggcgcg
    11881 cattggtacg aataagggtt tttttgagct cttggagggc gagaccctgt tggaaggctt
    11941 ggaacggacg gggcatatgg tggaatatca gtgccgaagc ggatattgcg gatcgtgccg
    12001 ggtgaagatt ttggagggaa gcgttactta tcgggagccg cctttggctt ttttggggcg
    12061 ggacgagata ctgccgtgct gctgttacgt cgagggggat gtcaggctgg attgcggttt
    12121 gtcgggggag gatgaggggc tgtctgacag tttgttgaaa taaaagccgt ccgaacattg
    12181 gttcggacgg ctttttgacg gtattaccgg ttatgcctga tgagtcggta aaaggtttca
    12241 ccgtcttgga tataacccag ttctacccgg gcgatttccg aaatggcttc ttgaccgttt
    12301 tccaaatcat agacttcggc ggcaagggaa tgattgcgta gggcgagtgt ctggtttttt
    12361 tcttcttgaa cggcaatctg ttctctcaga ctgctgttgc gtccgatgct gcctttgccg
    12421 aaccagaggc tgtattggca acagacaagt gcgaaggata aaacgacagt tacccacttc
    12481 ataccttaac cttttttatt tgcccagttg gtagaatgcg gctttgccgg ggtagtcggc
    12541 ggcttccgcc aattcttcct cgatacgcag cagttggttg tatttcgcca tgcggtcgga
    12601 acggctcaaa gaaccggttt tgatctgcat acagttggtg gcgactgcca agtcggcaat
    12661 ggtgctgtct tcggtttcgc cggagcggtg gctcatcacg ctggcgtagc ggttgcgttt
    12721 tgccagatcg acggctttca gggtttcact caaagtaccg atttggttga ctttgaccaa
    12781 tagtgcgttt gccacgcctt tttcaatgcc ttcggcaagg attttcgggt tggtaacgaa
    12841 gaggtcgtcg ccaacgagtt ggactttgcc gcccagtttt tcggtcagca gtttccagcc
    12901 ttcccagtcg ttttcatcca tgccgtcttc gatggagatg atggggaact cgttgaccag
    12961 gccttccaga tattcggcaa attccgcgtt ggtgtaggag cggccttcgg cttccaagtg
    13021 gtatttgccg tctttgtaga attcgctgga cgcgcagtcc agggcgaaca acacgtcttc
    13081 gcccgctttg tagccggcgg cttcggtcgc ctcgaccatc agttgcaggg cttctttgtg
    13141 gctgttcagg ttgggggcga aaccgccttc gtcgccgact gtggtcggga agcctttgct
    13201 gtcgcacagt tttttcagag cgtggaaaat ttccgcaccg cagcgcaacg cttcgcggaa
    13261 agattttgcg ccgacgggca taatcataaa ctcttggatg ttcaggctgt tgttggcgtg
    13321 ttcgccgccg ttgatgacgt tcatcatagg tacgggcagg gacatcgggc ctgcgccgcc
    13381 caagtagcgg taaagcggca ggcctgagtc ttcggcagcg gcgcgtgcaa ccgccataga
    13441 aaccgccaaa gtcgcattcg cacccaaatt gcctttgttt tcagtaccgt ccaattcgat
    13501 catgatttgg tcgatataag attgctcgtt ggcatcgata ccaatgaggg cttgggcgat
    13561 ttggttgttg acgtgttcga ccgccttcaa tacgcccttg cccgaataac gggatttgtc
    13621 gccgtcgcga agttccaaag cctctttttg accggtggac gcgccgctcg gtacggctgc
    13681 gcgtcccatt acgccggatt cgagcaatac atcacactcg actgtggggt tgccgcgtga
    13741 gtccaaaatt tcgcgggcga aaatatcaac gattgcgctc atgaatgttc tccaagtgaa
    13801 gtgaagggag gtaaaaaatg ggagggcttc agacgacctc tttgccggaa agccgccaac
    13861 ttaaaactgc tgaaagtagg gcggttgcca tgaaaatcat gctggaaata ccgtgccaca
    13921 ttccgaagga tccgcctaca aacgacaaca gccaattttc atgcccgtat ttcagtgcct
    13981 cgataacggg ggtaaccaaa aattggttgg cggcaagggc ggacaataaa aaaagcagca
    14041 gggcggtttg ccgccgggtt agggcggcaa aggcggcagc cagtaccgcg ccccaaacgg
    14101 caagcccgct ccaagagagg atgtcgaaca atacgccggc aatttcgccc gcctgcattt
    14161 tgggcagcat tttgaacagc accggcgcgg cgatataacc cgccataatc tgcataccga
    14221 gccacaaggt tgcggcatac aggctgattt ttcggaaagt ctgcatcata aggcgaaacc
    14281 gtgttactcg attgttaaaa tcggttgtga tttgattaaa tcgtccaatg ctttgatgcg
    14341 gattaaaaaa tcttctaaaa ggtgcagcgg cagcgcgctg gggccgtcgc attttgccag
    14401 tttcggatcg gggtgcgatt cgaggaacag gccggcaagg cgggttgcca tgcctgcaag
    14461 tgccaaatcc aaagcctgtg cgcgacgacc gccggatgcg gcagaaccgg catcgcgggt
    14521 ttgcagggaa tgggtaacgt cgaaaataac cggcaggtta ccgcaagtct gtttcatcac
    14581 gccgaaaccg agcatatcga caacgaggtt gtcgtagccg aagctgctgc cgcgttcgca
    14641 taaaatcagt ttcccgttgc cggcttcgcg gaatttttcc acaatgtttt tcatttgcga
    14701 ggggctgagg aactggggtt ttttgatatt gacgacctta ccggttttcg ccattgtggt
    14761 tactaagtcg gtttgccgcg caaggaaggc gggaagctgg atgacatcgc acacttcggc
    14821 gacgggttgg cactgatggg gttcgtgtac gtcggtgatg acggggatgc cgaactctgc
    14881 tttgactttt tcaaaaatct ttaagccttc ttccaagcct acgccgcgat aggaatggat
    14941 ggaggaacgg tttgccttgt cgaaagaggc tttaaagata tagggaatat cgagttttcg
    15001 ggtaacttcg acgtaatgcg cgcaggtttg gagggtggaa tccaaatctt ctaaaacgtt
    15061 gatgccgccg aataggacga agggcgagtt gttgccgatg gtgatgtcgt tgattttaat
    15121 atccataatc aatgtccgtt ttcagggtta cagtacggtg tgcggcggct cgtaggagat
    15181 gatgtcgcgg attttgttgt ttccgtctac caagacgact ttgggctcgt gtgcggcgat
    15241 ttcgggttcg gagagttgga cgtaagacat gatgatgacg atatcgcctt tctgcaccag
    15301 ccttgccgca gcaccgttca gacaaatcac gccgctgccg cgtttgcctg aaatggtata
    15361 ggtttcaaaa cgttcgccgt tgttgttgtt gacgatggcg actttttcgt tggggtagat
    15421 gcctgccgcg tctaacagat cttgatcgac ggtaatgctg ccgacgtagt tcaaatcggc
    15481 ttcggttacg gtggcgcggt ggatttttcc gccaagcata gtacggaaca tgtatttcct
    15541 tttggtttgt gtgcggtctg acgggcgcga tgccgtctgg aaatctgaat gccgtattat
    15601 agtggagttt ccttcaaatt cataatggcg gctttcggac aaggaaaacg gtgtttcaga
    15661 cggcattgcg gtgtcggctt tatttgggca ggcatctgtt ttgcaaggtt tttaaaacgt
    15721 tttttacggt gttgattctg acctcgatat tttccatctc tgcggtaaac cgcagcttat
    15781 cggcgccggc aaggcggtat tttttgtcgt tctgaatcag caggatgatt tcggttggat
    15841 cgacattatt gtttttaccg aaggttaccg ttaccgcttc gccggccgca tcaatggcat
    15901 cgatacccaa ttcttttgcc ataagccgta agtggtggct ttcgataagg gttttgacgg
    15961 gttgttcggg caggccgaag cggtcgacga gttcttcgtg tatggcgttg atttgctgca
    16021 cggtttcgca gacggcgagg cgtttgtaga ggacgagccg ttcgtggatg tcagggcagt
    16081 aatcttcagg cagtaatgtc gggctgtgca gtttgatttc ggtggtaatg cctaatggtg
    16141 cgtcgaggtc gggctgtcgg ccttttttga ggtcgcgcac ggcttgtttg agcatttcgg
    16201 tgtagagcgt gaagccgacc tgtatcattt cgccggattg tccttcgccg aggatttcgc
    16261 ctgcgccgcg gatttccaaa tcctgcatgg cgagggtgaa acctgcgccg agttcgtctg
    16321 ccgccgcgat ggcgtcgaga cgtttttctg cgtctttggt gatgtattcg ggcgtgagca
    16381 ggtaggcgta ggcttggtgg tggctgcggc cgacgcgccc gcgaagctgg tgcagttgcg
    16441 ccagtccgaa tttgtcggcg cggttgatga tgatggtgtt ggcgttggga atgtcgatac
    16501 cggtttcgat gatggtggaa cagagcaaca cgttaaatcg ttgctgcaaa aagtcgcgca
    16561 tgacttgttc cagctcgcgc tcgcgcagtt gtccgtgcgc cacgccgatg cgggcttcgg
    16621 gcagcagggt ttccagccgc tcgcgcatat tttcaatcgt atctacttca ttgtgcagga
    16681 aaaatacctg tcctccgcgt ttgagttcgc gcaacacggc ttcgcgcacg ctgccttcgc
    16741 taaagggttt gacaaaggtt ttgacggcga ggcggcggct gggcgcggtg gtaatcagcg
    16801 agaagtcgcg cagtccttcc aacgccatac ttaaagtacg cggaatcggc gtggcggtca
    16861 tggtaaggat atcaacattg gcgcgcaggc gtttgagctg ctctttctga cgcacgccga
    16921 agcggtgttc ttcgtcgata atcactaaac ctaagttttt gaatttgatg tcgtcctgca
    16981 ccagtttgtg cgtgccgata acaatatcga ccgtgccgtc tgccatgcct tccagcgcgg
    17041 ctttggtggc tttgctgttg ttgaaacgcg aaaggctggc gactttcacg gggaaatcgg
    17101 cgaaacggtc ggcgaagttt tgcgcgtgct gctcgaccaa aagcgtggtc ggagcaagta
    17161 cggcgacctg tttgccgccc atcaccgcca caaacgcggc gcgcagggcg acttcggttt
    17221 tgccgaagcc gacatcgccg cacacaaggc gatccatcgg cttcgcttgc gtcaaatctt
    17281 taatcacggc ggcgatggcg gcggcctggt cttcggtttc ctcgtagccg aagccgtcgg
    17341 caaacgcctg atagtccaac tcgttgattt caaacttgtg tcccgattgg gcggcgcgtt
    17401 gggcgtagag gttgagcaat tcggcggcgg tgtcgcgcgc tttttcggcg gctttgcgct
    17461 tcgccttgtt ccacgcgcca ctgccgagct tgtgcagggc aatgttttca tgcgcctgac
    17521 cggagtagcg gctgattaaa tgcagttgcg aaacaggcac ataaagctgc gcttcgcctg
    17581 cgtattcgag caacatcatt tcgttggttt cgccgcccaa gtccatcgtt accaagccca
    17641 tataccgccc gatgccgtgt tcttcgtgaa cgacggggtc gccgatgttg atttcggcaa
    17701 ggtcgcgcaa cagaccgtct gaaacggcgg cgtgtttctt gcggcggttg tggacgcgcg
    17761 aacgggcgac gtattggtag agatcggatt cggtgatgac ggcgatggca ctttgagcgg
    17821 caactgcttt gccctctcc
//
LOCUS       DAWFJW010000034        17736 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFJW010000034 DAWFJW010000000
VERSION     DAWFJW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17736
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.034"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..675
                     /locus_tag="WGR19_000927"
     CDS             <1..675
                     /locus_tag="WGR19_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0798477.1"
                     /translation="YAAERITHDYPKASGANNTSTVSDYFRNIRAHSIHPRVSVGYDF
                     GDWRIAADYASYRKWNNNKYSVNTKEVRRNNNAGNWQELKTENQENGSFHASSSLGLS
                     AIYDFKLNDKFDKFKPYIGVRVAYGHVKHQVHSVKKETTTTFLAPTGDAKVPGKIVEG
                     PFSKPAYHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSL
                     GMRYHF"
     gene            complement(649..858)
                     /locus_tag="WGR19_000928"
     CDS             complement(649..858)
                     /locus_tag="WGR19_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798478.1"
                     /translation="MPSERLSDGIFPCVLNQVHETPDAFGGNVGCRTMFGGDQRTKCA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(876..1433)
                     /locus_tag="WGR19_000929"
     CDS             complement(876..1433)
                     /locus_tag="WGR19_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0798479.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1509..3143)
                     /locus_tag="WGR19_000930"
     CDS             complement(1509..3143)
                     /locus_tag="WGR19_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0798480.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3545..4651)
                     /gene="serC"
                     /locus_tag="WGR19_000931"
     CDS             complement(3545..4651)
                     /gene="serC"
                     /locus_tag="WGR19_000931"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0798481.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            5016..5447
                     /gene="rimP"
                     /locus_tag="WGR19_000932"
     CDS             5016..5447
                     /gene="rimP"
                     /locus_tag="WGR19_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0798482.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5475..6977
                     /gene="nusA"
                     /locus_tag="WGR19_000933"
     CDS             5475..6977
                     /gene="nusA"
                     /locus_tag="WGR19_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0798483.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6989..9877
                     /gene="infB"
                     /locus_tag="WGR19_000934"
     CDS             6989..9877
                     /gene="infB"
                     /locus_tag="WGR19_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0798484.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPVEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEASPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGENQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(10088..11482)
                     /locus_tag="WGR19_000935"
     CDS             complement(10088..11482)
                     /locus_tag="WGR19_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEZ0798485.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSHAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11556..12896)
                     /locus_tag="WGR19_000936"
     CDS             complement(11556..12896)
                     /locus_tag="WGR19_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0798486.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPMQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRLVEMLAWLPS
                     KLGFPVPDARAVVEGRLNGNIADAQAWSGLLVGSIACYGILPRFLAWVVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKDQ"
     gene            13039..13665
                     /locus_tag="WGR19_000937"
     CDS             13039..13665
                     /locus_tag="WGR19_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0798487.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13729..15147)
                     /locus_tag="WGR19_000938"
     CDS             complement(13729..15147)
                     /locus_tag="WGR19_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0798488.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            15474..16202
                     /locus_tag="WGR19_000939"
     CDS             15474..16202
                     /locus_tag="WGR19_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0798489.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16261..16749)
                     /locus_tag="WGR19_000940"
     CDS             complement(16261..16749)
                     /locus_tag="WGR19_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0798490.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGTVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVALTVWWAWAR
                     AK"
     gene            complement(17506..>17736)
                     /locus_tag="WGR19_000941"
     CDS             complement(17506..>17736)
                     /locus_tag="WGR19_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ0798491.1"
                     /translation="GNIFEDFKKEVLKIPQIMECHLVSGEYDYLIKVRLPDMSAYRDM
                     LGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4695 a         4961 c         4451 g         3629 t
ORIGIN      
        1 tatgccgccg aacgtattac ccacgattat ccgaaagcat ccggtgcaaa caacacaagc
       61 acagtaagcg attatttcag aaacatccgt gcgcattcca tccacccccg ggtgtcggtc
      121 ggctacgact tcggcgactg gaggatagcg gcagattatg ccagttacag aaaatggaac
      181 aacaataaat attccgtcaa cacaaaagag gtgagaagaa acaataacgc tggcaactgg
      241 caagaactga agacggaaaa tcaggaaaac ggcagcttcc acgcctcttc ttctctcggc
      301 ttatccgcca tttacgattt caaactcaac gataaattcg ataaattcaa accctatatc
      361 ggtgtgcgcg tcgcctacgg acacgttaaa catcaggttc attcagtgaa aaaagaaacc
      421 acgactactt tccttgcacc aacgggagac gctaaagtgc caggcaagat cgtagaaggt
      481 ccgttcagca aacctgccta tcacgaaagc cacagcatca gcagcttggg tcttggtgtc
      541 atcgccggtg tcggtttcga catcacgccc aagctgacct tggacaccgg ataccgttac
      601 cacaactggg gacgcttgga aaacacccgc ttcaaaaccc acgaagtctc attgggcatg
      661 cgctaccact tctgattccc cgataccgat gccgtctgaa ccttcagacg gcatgagacc
      721 tttgcctgcg cacttggtgc gctggtcgcc tccgaacatg gtgcgacacc cgacatttcc
      781 gccgaacgca tcgggcgttt catgaacctg gtttaaaacg catggaaaaa tgccgtctga
      841 aagcctttca gacggcattt tgcttgagat tccgtttacc aatggctgac aaacgcttcc
      901 aaatcggtat tcttgggctt atgcacttcc tctgtcggcg tgccgaccat catcagcccg
      961 atgattttat ccttatccgc acatccgaaa gcctcacgca acagggggct gttgacccac
     1021 atccccgtta tccagacatt gtcgaaacct tgcgccgttg ccgccagttg cagcgcatac
     1081 gccgcacacc ccgccgtcag catctgctcc cattccggtt tcggcttagg cacatcgcgg
     1141 ttcggcgcaa acgttacccc gatgaccatc ggcgccatat tgcccacttt ttccgccttt
     1201 ttcatcgcat cgtcgccgaa attcaattcg gcaaccgttt gctttaacac atcgcgaaaa
     1261 cgttgcaatc ctacctcgcc ttgaatcacg gtaaaacgga aggggcgcat attgccgtga
     1321 tcgggaactt gggttgccgc ctgaaatatt tgttccaact ccgccgcatc gggggcgggg
     1381 tgcttcagct ttttggaaga tcggcggttc gtcaataatt ttaaagcatc catatcgtta
     1441 tactccggtc atcggtcggg tgttcggaca catatgccgt ccgaaggctt cagacggcat
     1501 atccggcatc agcgcggacg gcggcaggct gccaatatat ccatttcctt ccgataggtt
     1561 tggctgttgg aaatgtccat cagccccaat accgtgctga aatagtggtc gtgcgaatat
     1621 tcgttttccg ccgctttttg tttgaggcat tggaaatcta tgcccccgtg ttggcggaag
     1681 gctttggaaa accacataac catcgggata tgcgtctgcc cggaaggcgc gatggcgtaa
     1741 ggcgcggcgt gcaggtacat cccgttttcg cccaaacttt cgccgtggtc ggaaacataa
     1801 tgcaccacgc tttccaaatc gtcgcggttt tcaagtttgc ggataacctt gtcgataaac
     1861 tggtccacat acaaaaccgt attgtcgtaa gtgttgacca gcgtggcgcg ggtgcatttg
     1921 ttgatttcgt tggtgtcgca ggtcggcgtg aatttgcgtt cggcttcggt atagcgttcg
     1981 taatacgtcg gcccgtggct gccgatggta tgcaggatta aaaccgcgtc tttatcgttt
     2041 ttgttgagga cttcgtcgaa cttagtcagc aggatattgt cgaggcactc gccgttgcgg
     2101 cagtattcgg gcaggttgag cgaggtaacg tcggtattcg gcactttgcc gcacacgccc
     2161 ttgcagccgg aatcgttttc caaccaggta acttccacgc cggcgcgctg cacgatgtcc
     2221 agcaggttgt cttggtgttc ggctttgatt tcgtcataat ccgtgcggtc gaaagttgag
     2281 aacatacacg gcagggagtg cgcggtcgat gtaccgcagc ttctgacctg cgggaaattg
     2341 acaatttcat cgccgcgcgc ggcaagcagc ggcgtagttt ggcggctgta accgttcaaa
     2401 ccccagttgg cggcacgcgt ggtctcgccc acgaccagca ccacgaaacg gcgcaggctg
     2461 ccggccggcc ggttttgcac gaccgccata tccaattgcg tataaggaat attggaacgc
     2521 ttccaatctt tgtatttcga cacgcccgcg ccgatgaaat tagacggcac aatcagatgg
     2581 gttactgatt tattgttgcg gaaaaacgag gcgtaatcct gatattgcaa cattgcgata
     2641 cccaacgcgc acaaaaagga aacggcggca agcacaaggc gcgtcaaaag ctccttatac
     2701 caaacgcggt atttaacctt gacggcgata tacgccagcg cgggcaatac gcccaaacat
     2761 acaatccaca gcacatagcc cggcgtaatc aggcgcgcgc tttcggcagc cgtagtttgc
     2821 aagacattat tcaacatcga cttgttgaaa tagatattga aaaatatttc ttggtaagac
     2881 accgccgcac tgataaccaa tatcaacgga atcaatacct tatgcacgaa aggcagggca
     2941 atgacgtgaa aaacgaaatt acttaaaaaa aacagcacca ccggcatcgt atagaggaag
     3001 atatccgccc cggtgccgtt aaaaggatga agctcgacaa ctttggcaaa aaaggcgtaa
     3061 ttcaatacca gcgaggaata cagggaaagg aaggcaatca gcgcggaaga gccgagcttc
     3121 ggcctcaggt ttggttttat catttggaat gtgtcggata agggttggaa aaggcatccg
     3181 gcatttggaa tccggattat tgaaaaagat tcttaattat aaggcaacgg agcaaagcag
     3241 ggcaagaaaa cggcggctgt gcgggggttc cgcctgccat tcaaacgtcc ggcagacata
     3301 aaaacatcgt aagcaagatt cgaaccggtc tgcaaccgcc cctgccagaa aaacgggcaa
     3361 agcatttcat attggaaaaa cccgaccgcg ccgccgacgg gacagtccgg cacaaacagc
     3421 atcacgctcg gattgaaaag gacggatagc cgccggcagc acaatcttac cacctccaaa
     3481 acgccgccgg agaacgccga aacagccgat gccgtctgaa gccgcttcag acaacatcgg
     3541 gacatcaacc gtaacgccgt tggaaatcgc gcataaaatc tgccaaagcc cgcacgcctt
     3601 caagcggcat cgcattataa atgctggcgc gcatcccacc gacagactta tagcccttca
     3661 acaggcacaa gccctgcaat tcggcttcca gcacaaaacg gcggtcaagc tcctcatccc
     3721 ccgtttggaa cacgacattc attttagaac gcgcattcgg acggatacgg ttgatataga
     3781 aaccgccgct gccatctatc gtctcataca aggtttgcgc cttcagccga ttgaccgctt
     3841 caattttttt cacaccgccc tgcgcctgca gccagcggaa caccagcccc gacatataaa
     3901 tcgcgtaagt tgacggcgtg ttgtacatac cttcgcggtt caaatgcgaa cggtagttga
     3961 acacatcggg aatatcgttc ggacaacgct cgagcaaatc ctcacgcaca atcaccaccg
     4021 taacccctgc cgggccgatg tttttctgtg cgcctgcgta aatcagtccg tagtcggcaa
     4081 catcaaactc gcgcgacaaa atctcgctgg acatatcgca caccagcggc ggcatgcctt
     4141 ctgaaaggcg cggcacttca cggtattgca gcccgttgac cgtttcattg acggcaaaat
     4201 ggacaaacgc cgaatcgggt gcaacatccc acgtttccac aggcggcagg tcgagatagt
     4261 cgaactgctc gccgccatgc gccgccaaac ggatttccgt atcggtcaaa cggctcatct
     4321 gttcataagc gatacggctc cagttgcccg ttaccaccgc gtcggcagtg cggaaaccgt
     4381 gtgccagatt catggctgcc atattaaatt gggttgttgc tccgccctgc agaaacaata
     4441 tcttatagtt gtcaggcact ttcaaaagct gcctcaaatc ctgttccgca tgatgcagga
     4501 tgctcaaaaa catttccgaa cggtggctca tcgccatcac gggaaaaccc gtaccgttgt
     4561 agtccaacat ttcctgccgc gccgtttcca acacggcttc gggcaatacg gcagggccgg
     4621 cggaaaaatt gtaaatcgga taaagagaca taatgcagcc ctgattctga acaataaccc
     4681 gcctgatttt aggcttgcag caggctttgt gcaaggatgg gaaatacctg tcctccgccc
     4741 ggcttcatgc cgcccgaaca cggaaaacaa catcaatata ttgatttaca aacataaaaa
     4801 tcatgctagc gacaaataga tacatttgtt ttgtcaacaa tattcacgat ttcccattac
     4861 aaacctccct tacacccgct tttttccgtc ccaaaaacac aaaataaatc aacactttca
     4921 tttctccgca aaagcggtta taatcacgcc gatttttcaa ctttgacgaa aaatggccac
     4981 ggggccattt ttttattatg tacataggga gcagcatgga tattcaaacc atcctcgaaa
     5041 aaaccctgcc cggcctgggc tacgaactgg tcgatttcga actgaccgcg caaggaacat
     5101 tgcgcgtgtt catcgacaaa gaaggcggca ttaccgtcga agactgcgca accgtcagca
     5161 accatttgag ccgcgtcttc atggttgaag acatcgacta caaaaacctg gaaatttcca
     5221 gccccggact cgaccgcccc ttgaaaaaag ccgccgactt cgtgcgcttt gccggtcaga
     5281 atgccaaaat caaaacccgc ctgccgatag acggtcagaa aaactttatc ggtaaaatcg
     5341 aaggctgcga aaacgatacc gttaccgtat ccttcgacgg caaaaccgta caaatcgaat
     5401 tgggcaacat cgacaaagcc cgcctgcgcc ccgaattcaa attctaaaac acaacaatat
     5461 tggagatgtt caaaatgagt cgtgaaatgt tacagctggc agaagcactg gcaagcgaaa
     5521 aaaacgttga tgcggaagtc gtcttccaag cactggaatt cgccctgtct accgccgcca
     5581 agaaaaaggc agaccgcgaa cacatggatg tccgcgtaca aatcaaccgc gacaccggcg
     5641 aataccaaac cttccgccgt tggctgattg tcgccgatga agactatacc tatcccgatg
     5701 tcgaaaaaac catcgaggaa atccaagagg aaattcccgg caccgccatc caaatcggcg
     5761 aatactacga agagcagctg cctaacgaag gcttcggccg ccaagccgcg caaaccgcca
     5821 aacaaatcat cctgcaacgc atccgcgatg ccgagcgcga gcaaaatctg aacgagtttc
     5881 tcgccgtcaa agaagacatc gtgtccggta cggtcaaacg cgtcgaacgc cacggcatca
     5941 tcgtcgaagt cgttgccggc aaactggacg cgctgattcc gcgcgaccaa atgattccgc
     6001 gcgaaaactt ccgcagcggc gaccgcatcc gcgccctctt cctgcgcgtc gaagaaatcg
     6061 gcaacaccgg ccgcaaacaa gtcatcctga gccgtacttc cggcgatttc ctcgtcaaac
     6121 tgtacgccaa tgaagtacct gaaattgcag acggcatgct tgaaatccgc gctgtcgccc
     6181 gcgacccggg acaacgtgcc aaagtcgccg tcaaagccaa cgaccagcgc atcgatccgc
     6241 aaggcacctg tatcggcgtt cgcggttcgc gtgtcaatgc cgtcagcaat gaattgtccg
     6301 gcgagcgcat cgacgttgtc ctctggtcgc ccgaacccgc gcaattcgtg atgagcgcgc
     6361 tctcacccgc cgaagtcagc cgcatcgtca tcgacgaaga caaacacgcc gtcgatgtca
     6421 tcgttgccga agaccagctc gcgctcgcca tcggacgcgg cggtcaaaac gtgcgccttg
     6481 cttccgacct gaccggctgg cagctcaaca tcatgacttc cgccgaggca gacgaacgca
     6541 atgcggcaga agatgccgcc atccgccgcc tgtttatgga tcacttgaac gtggacgaag
     6601 aaaccgccga cgtactggtt caggaaggtt ttgcaacctt ggaagaagtc gcctatgttc
     6661 ctgccgccga actgcttgcc attgaaggat ttgacgaaga aatcgtcgat atgctccgca
     6721 accgtgcccg cgatgccatc ctgaccatgg cgattgccgc cgaagaaaaa ctgggcgaag
     6781 tatccgacga tatgcgcaac ctcgaaggca tagatgccga tatgctccgc agccttgccg
     6841 aagcaggcat taccacccgc gacgacttgg cagagcttgc cgtggacgaa ctgattgaaa
     6901 tcaccggtgt aaacgaagaa accgcaaaag ccgtcatcct gaccgcacgc gaacactggt
     6961 ttaccgaaga caaataaagg gggtacagat gagtaacaca accgtagaac aatttgccgc
     7021 cgagctgaaa cgccccgtcg aagacctgtt gaaacagttg aaagaagccg gcgtcagcaa
     7081 aaacagcggc agcgattccc tgacgctgga cgacaaacag cttctgaacg cctacctgac
     7141 caagaaaaac ggcagcaacg gcggcaccat cagcatccgc cgcaccaaaa ccgaagtcag
     7201 caccgttgac ggcgtaaaag tcgaaacacg caaacgcgga cgcactgtca acattccttc
     7261 tgccgaagaa ttggcagcac aggtaaaagc cgcccaaacc caagccgcac ctgtccggcc
     7321 ggagcagacg gcagaagacg cggcaaaagc ccgagccgaa gctgccgcac gtgcagaagc
     7381 ccgtgccaag gcagaagcgg aagcggcaaa actgaaagcg gcaaaagcag gcaacaaagc
     7441 caaacctgcc gcgcagaaac ccaccgaagc aaaagccgaa accgcacccg ttgcggcgga
     7501 aaccaaaccc gccgaagaaa gcaaagcgga aaaagcccaa gccgacaaaa tgccgtctaa
     7561 aaaacccgtc gagcccaaag aaaaagccgc caagccgaaa cacgagcgaa acggcaaagg
     7621 caaagatgcc aaaaaaccgg cgaaacctgc cgcacctgcc gtgccgcaac ccgtggtcag
     7681 cgcggaagaa caggcgcaac gcgatgaaga agcacgccgt gccgccgcac ttcgcgccca
     7741 ccaggaagcc ctgttgaaag agaaacagga acgccaggca cgccgcgaag ccatgaaaca
     7801 acaggcagaa caacaggcaa aagccgcaca ggaagccaaa accggcagac agcgtcccgc
     7861 caaacctgcc gagaaaccgc aggcagccgc gccggccgtc gaaaacaaac ctgtcaatcc
     7921 ggcaaaagcg aaaaaagaag accgccgcaa ccgcgatgac gaaggtcaag gccgcaacgc
     7981 caaaggcaaa ggcggaaaag gcgggcgcga ccgcaacaat gcacgcaacg gcgacgacga
     8041 gcgcgtacgc ggcggcaaaa aaggcaaaaa actcaaactc gagccgaacc aacacgcctt
     8101 ccaagcaccg accgaacccg tcgttcatga agttttggtg cctgaaacca ttaccgttgc
     8161 cgatttggcg cacaaaatgg cggtcaaagg cgtggaagtg gtcaaagccc tgatgaagat
     8221 gggcatgatg gttaccatca accaatccat cgaccaagac accgccctga ttgtcgtcga
     8281 agaactcgga cacatcggca aacccgccgc agcggacgac cccgaagcct tcttggacga
     8341 gggtgcggaa gcagtggaag ccgaagcatt gccgcgtccg cccgtcgtta ccgtgatggg
     8401 tcacgttgac cacggtaaaa cctcgctgct ggactacatc cgccgtacca aagtggtaca
     8461 gggcgaagca ggcggcatta cccaacacat cggtgcatac cacgttgaaa ccccacgcgg
     8521 cgtgattacc ttcttggata ctccgggtca cgaagccttt accgctatgc gcgcacgcgg
     8581 tgcgaaagca accgacatcg tgattcttgt ggtcgccgcc gacgacggcg tgatgccgca
     8641 aaccatcgaa gcgattgccc acgccaaagc ggcaggcgta ccgatggtgg ttgccgtcaa
     8701 caaaatcgat aaagaagccg ccaacccaga gcgtatccgc caagagctga ccgcacacga
     8761 agtcgtgcct gacgaatggg gcggcgatgt acagtttatc gacgtttccg ctaaaaaagg
     8821 tctgaacatc gatgcattgc tcgaagccgt attgctcgaa gccgaagttt tggaactgac
     8881 cgcacctgtc gatgcgcccg ccaaaggcat tatcgtcgaa gcccgtttgg acaaagggcg
     8941 cggcgcggtt gccacattgc tggttcaaag cggcacgctg aaaaaaggcg atatgctgct
     9001 ggccggtacc gcgttcggca aaatccgcgc gatggtcgat gaaaacggca aatccattac
     9061 cgaagccagc ccgtccatcc ccgtcgaaat cctcggcttg tccgacgtac cgaatgcggg
     9121 tgaagatgcg atggtattgg cggacgagaa aaaagcgcgc gaaatcgccc tcttccgcca
     9181 aggcaaatac cgcgacgtgc gccttgccaa acagcaggcg gcgaagctgg aaaatatgtt
     9241 caacaatatg ggcgaaaacc aagcccaatc tttgtcggtc atcatcaagg cagacgtgca
     9301 aggttcttac gaggctttgg cgggcagcct gaaaaaactg tccaccgacg aggtgaaagt
     9361 gaacgtgttg cacagcggcg tgggcggcat taccgaatcg gatgtcaacc ttgccatcgc
     9421 ttcgggcgcg ttcattatcg gctttaacgt gcgtgcagat gcctcttcgc gcaaacttgc
     9481 cgaaaatgaa aacgtggaaa tccgctacta caacatcatc tacgatgcca tcgacgacgt
     9541 gaaggcggcg atgagcggca tgctttctcc ggaagagaaa gaacaggtta ccggtacggt
     9601 cgaaatccgt caggtcatct ccgtttccaa agtcggcaac attgcaggct gtatggttac
     9661 cgacggcgtg gtcaaacgcg attcccatgt ccgcctcatc cgcaacaacg tagtcatcca
     9721 cacgggcgaa ctggcttcgt tgaaacgcta taaagacgac gtaaaagaag tccgtatggg
     9781 cttcgagtgc ggtctgatgc tcaaaggcta caacgaaatc atggaaggcg accaactgga
     9841 atgcttcgac atcgtcgaag tggcacgcac cctgtgattt tgatgcggaa aaatgccgtc
     9901 tgaagcgttc agacggcata cggaacgggt tctgtatcat acagaacccg ttttttatcg
     9961 caaatcggct tcagacagcc ctcctgcctt atcccgattt gaatctgact tgccatacaa
    10021 acaggcttca gacggcatta tagtgaatta aatttaaacc ggtacagcgt tggctcgcct
    10081 tgccgtacta tttgtactgt ctgcggctcg ccgccttgtc ctgatttaaa tttaattcac
    10141 tatattttcc cgacaggcgg atttgaagtt tctccacata tccggcgcgt tcggcgtggc
    10201 tggtttcggg gcggtgcgta ttgagcgacg accatttcca atgactgcga gctttgttga
    10261 gttcgggcgg gagtctggcg gcgtcccacg gggctttgcg gctgtgcagc tcaatatccg
    10321 actgtgccgc gtgtccgcgc gtttgcagga cgtggagcag atcgagggcg cgggcggcga
    10381 gcagggtcag ggtttcgggg tcggtgtgca aggtttggcg gccggcgagt ttgtcggaaa
    10441 tggtgcgggt attgggcagg atgccgccca aaaagccgcc gattgccgta cccaatccga
    10501 gcgagccgcc gagtgtggcg atgtccagcc ccaagccgat gagcgcgccg gttgccgcgc
    10561 ccgtgccggt gcggatgccg tattgtttga gcaattcgct gtcgaacggg tcttggcgga
    10621 aggcttgcgg catccagtcg ccgccgtcga tttcgctgtg gtagaaacgg tagagggcaa
    10681 acagccgctg ctgcatctgc cgttcgagtt ggcgtatttc cgcctgcatg gtttgcagca
    10741 cggtggcggt atcctcgttt tcgtccactt cctgcctgaa ggcggcggca tcgagcagga
    10801 aatcggcgat ttcgcggcgc gcttcgccgt ccagccgctg ccattcgcgc cggcgcatgg
    10861 ctgtcaggcg gtcgagtgtg ctgcgttcgg gcaacatggt ggcgaggttt tcccacaggc
    10921 gcagttcgcc ttcaaaatca aaggcgacgg tgtcgaaccc tgcgaaaacg tgcaggtttc
    10981 tcctcgccag catggttgtc cacgattcgg gaggctgtcc gccggtaaag ttgaacacgg
    11041 gcataaccgg tttggcgcac catgaaagga taatcaattc atcccgatat ttgtcgagaa
    11101 cgggttcgcg cgcgtcgatg acgtacattg ccatatcgct ttgcaagact tgccgtaaga
    11161 ctttggcttc ctgattgaaa tcatggtgcg cgccgtggct gccgagaaac tgttgcagcc
    11221 gttcgatgcc gtctgaacga ttgtccgtat ggttttccag ccattccagc acgccgcccg
    11281 cgtcttcgag tccgggcgtg tcgtacagga aaaccagcgt gtctgcgccg tcgctgatgg
    11341 cggcttcttc gacatgacgc gtggtcgatg gggcgtttct gacttcgccg aaaccgctgt
    11401 cgcgcaatag ggtgcgcagg agcgaggttt tgccggtgtt ggtgtgtccg acgacggcga
    11461 gggaaagggg ttgtttgttc atgatgtttt tgaagaatgg attttcagac ggtctttttt
    11521 cagaatggcg gcttaacaga acatttcaag tgagtttatt ggtctttcaa acggccttcc
    11581 tgcgccgccc tgtcaggctc aagccacgcc gcgccgcatt cggtcagcgc gttacgccaa
    11641 tgttccagct tttccgaaag gtcgtctgaa agcccctgtt ccgccaaaag ctgcaccacc
    11701 gcgccgccct gcgccgcttc cgaaagtcgg acgatctgcc gcaacacgcc gcggtcgggc
    11761 acagtttggg cgcgcacgcc gataagcagt tgcgccggtt tctgcttcag ctctgtctcc
    11821 agcgcggcaa cctgttcccg attggtggca acgcccttat ccagccattc ctgcgccagc
    11881 ctgccctcga accattcgcc gtcctgccac tcggtctcca gcatgaccgc ccatttcggc
    11941 gcatcgttca agacgatttt cggcgaaacg gcggacacgg tttcccgacg cgtatccgca
    12001 tcggtaattt tgttctgcca gcggcggatg accgcctgat aatagggttt ttccaaatcc
    12061 aagccgtttt tgcttgtttt caaaaggatt ttacacacca cccaagccaa gaagcgcggc
    12121 aggatgccgt agcaggcgat actgccgacc agcagccccg accaagcctg cgcatcggca
    12181 atattgccgt tcagacgacc ttcaactacc gcccgcgcat cggggacagg gaaaccgagt
    12241 ttcgacggca gccatgccaa catttccacc agccgtaccg aagcggcatt gctcaacagc
    12301 gtgctttccc agttgaacgt atattggcgc accaaaagca gcaacaatac cgacaccagc
    12361 attccgagca gcgtgcagag ccacaggctg tgcgacgttg cgcctatttt ccaacgtacc
    12421 gaaggttgcc gccactcgtc cgcatacagc cgcaacaccg cctgatttac agggtctttg
    12481 ccccgaaacc acgtcgccgg actgctgaaa aaacgcccca ctttcacgcg caggaacaac
    12541 attgccaacc atactgccag catcagcgta ttcatgccca acacgcccgc caaaaccaaa
    12601 aagaaattca gaccctgatt gtccattaga agataagtac ctgaaaaacc ggtaaaaaac
    12661 gcaaacgtcg ccgccgccac ccacaaccag aacgaccccg cacgcacacg ttccaatgtg
    12721 tcccgcagca tacggttcct gtcgatcatc tccgcccgac ggatgatttt ttcctccgta
    12781 ctgccgtcca cgcggcgcaa agcctccgtc gcctgcatgg gatcgccgct gaaaataaaa
    12841 ccgccttcgt ccaaaatacg gatcagctca accagttttc gggatggatt caacataaaa
    12901 tgccgtctga aaataaaaaa cagattttaa cacacgcatt ttcaagaata ttcacagtgt
    12961 aggcaaagag taaatctcac acagaggcaa aagtatcggc gtaaactgac tgcctctact
    13021 ttcccgaaag attgtgcgat gtatacaggc gaacgcttca atacttacag ccatttgagc
    13081 ggtttgattc tggcggcggc aggtttggcg ctgatgctgc tgaaaaccat aggacacggg
    13141 gacggctacc gtatcttcag cgtatcggtt tacggcatca gccttcttct gctctatttg
    13201 agttcctcgc tgtaccacgg aattgcagcc ggaaaactga aaagcatttt gaaaaaaacc
    13261 gaccactgca tgatttatgt gctgattgcc ggaagctaca caccgtttgc actggtttct
    13321 ttgagaaacg ggccgggctg gacggtattt tcactgtcct ggctgctggc ggctgcagga
    13381 atcgcacaag aactcaccat cggacggaaa agcgaaaaac gactgctgtc tattgtgatt
    13441 tatgtcgtca tgggttggat ggtcttggcg gtaatgaaat ccctgacagc ctcactcccg
    13501 ccggcaggac tggcttggct ggcggcaggc ggtatgctgt acagcgtcgg catttactgg
    13561 tttgtaaacg atgaaaaaat ccgacacggg cacggaatct ggcatctgtt cgtattgggc
    13621 ggcagcatca cccaatttgt cagcgtgtac ggttatgtaa tctgaatgcc gtctgaaaag
    13681 caaaacctcc cgatcctgaa gattgggagg ttttctgttt gccggacatc agcccttgtc
    13741 gtggaactcg tggaattcat acggataaga caaatcccga cccgcttttt tctgtgccaa
    13801 ataatcatca taaatggcac ggatttcctt acgcaacaaa aacagggcta tcaagttcgg
    13861 gataaccata aagccgttga acatatcgga caggctccaa accaaatcga ctttgccgag
    13921 cgtacccaaa acgatggcaa gcagaaccaa tgcgcgatag atgcccaagt gtcttcccct
    13981 gaaaagaaaa cggatgttgg actcaccgaa ataataccag ccgataatgg tggtgaaggc
    14041 aaagaaggtc agacacacgg caagcaattg cgaaccgaat cccggaaatg ccttgttaaa
    14101 ggcaaattga gtaaccgccg cgccctgttc gcccgaaagg ttggcatcgg tcagcaggat
    14161 aatcaatgca gttgccgtac ataccaaaat cgtatcgata aacacaccga caaatgccgc
    14221 cataccttgc tgcacaggat gcttcacatc cgcagtcgcg tgggcgtgcg gggtagaacc
    14281 catccccgct tcgttggaaa acagaccgcg cgccacgccg aaacgtatcg cttcgcgcat
    14341 accgataccc gccgcaccgc ccaaaacggc ttcaggattg aaggcggcgg taaagatgtg
    14401 gttgaacatc ggcacaatat agtcggaaaa ttcaaacagg ataacgacgg cgcacaaaat
    14461 ataaacaacc gccataaacg gcacgacaaa ctgggcaata ttggcaatac ggttcacgcc
    14521 gccaatcaca accatgcccg caaggacggc aagcacaata ccgactgcca aagaaggcac
    14581 atcaaatgca atggtaacgg cagaagcaat ggagtttgcc tgtgtcgcat taccgataaa
    14641 gcccaatgcg ataatcaacg caatggagaa aaagcccgac aggaaacgcg ccgcgcccct
    14701 gccgattttc ggagtcagac cgtgcgtaat ataaaacgcc ggcccaccga tgtatttgcc
    14761 gtggctgacg acgcggtatt tctgcgccag cagtgcctcc gcaaaaatcg tggacatccc
    14821 caaaacggca gaaacccaca tccaaaaaat cgcgcccggc ccgcctgcgg tgatggcggt
    14881 cgccacgccg gcgacgttgc ccgtaccgat ttgcgcagat atggcaaccg ccaacgcctg
    14941 aaactgcgat aaagacttgt cgtctttatc gcctttggca aacaagccgc cgaatacaga
    15001 tttgaatccc gcacccagct tggtaatctg cggcgcaccg agatacagcg taaaaaacag
    15061 gccgataccc aaaagcgcgt aaatcagcag gtagtcccaa aggaaccgat tgactgtacc
    15121 caccagaaca gacaatatat tttccataaa ataaacctta tcttacaatt aaaatgactg
    15181 ccttccaaaa gacattccaa taaggaaaca cggcgagcag accgtatttg ccgcaacaga
    15241 tgccttaaat tgtcaacaat cggggagaag ctgcgccatc ataccgtaaa atatcgttaa
    15301 ttaaaacatt tctttatttt taagcggaaa gcggaggaaa tcgctttcag acggcatcga
    15361 caacggcacg gcataaaaca ggatattttg ggtacttgca acttatgtta aaatgccgac
    15421 cgtaaaaaat ctgacaaaaa cagattaatt atttgaaata agaaaggaaa tttatggcag
    15481 gccatagcaa gtgggcgaat atccagcata aaaaagcccg tcaggatgcc aaacgcggca
    15541 aaatcttcac ccgtttaatc aaagaaatca ccgttgcggc gcgtatgggc ggcggcgatc
    15601 ccggcgcaaa tccgcgcctg cgcctggctt tggaaaaagc agccgaaaac aatatgccca
    15661 aagacaatgt gcaacgcgcc atcgacaaag gcacgggcaa cttggaaggc gtggaataca
    15721 tcgagttgcg ctacgaaggc tacggcatcg gcggcgcggc tttgatggtg gactgcctga
    15781 ccgacaacaa aacccgcacc gttgcggacg tacgccacgc gtttaccaaa aacggcggca
    15841 acttgggtac cgacggctgc gtggcgttca acttcgtgca tcagggctat ttggtattcg
    15901 aacccggcgt tgacgaagac gcgctgatgg aagcggcttt ggaagccggt gcggaagacg
    15961 tggttaccaa cgacgacggt tccatcgaag tcattaccgc gccaaacgat tgggcgggcg
    16021 taaaatccgc tttggaggcg gcaggttaca aatccgttga cggcgacgtt acgatgcgcg
    16081 cccaaaacga aaccgaactc tccggcgacg atgccgtcaa aatgcaaaaa ctgattgacg
    16141 cactggaaga tttggacgac gtgcaagacg tttacacttc cgccgtattg aatttggact
    16201 gatacgcagc acagcagaca tacaataaaa tgccgtctga acctttcaga cggcattgat
    16261 ttatttagcc cttgcccacg cccaccaaac cgtcagggca accgccagaa aatacgccac
    16321 actccaaaca ggcaaagcaa cccccagcag ataatccggt tcggcacaat ttccgaaccc
    16381 gcgcacgaca ggctcgaacc aatcaaacaa aggccagccc ttcaatcgaa acgtccacgg
    16441 cgcaccgcac gaaggagccg tacccggcgg cagcgactgc agccacaact gatatgccgc
    16501 aacagaaata cccgtaacgg ccggaatgct gataaagaca gtaccgaaca aaccgcccgc
    16561 ccttcttctt ggtctgcaca tcaggacaat tgccgtacac aatgcggttg ccaaaacgca
    16621 taagcgctga ctgatacaca aaacgcaagg ctccataccc aaaacatact gtgccgccaa
    16681 agaaccggca aatgcacaga ccgaaacggc aaacagcagc caaacggctt ttctaaataa
    16741 cggggtcatt ttctcaacac accaatcaaa ataccgatat gccgattttg ctggatatat
    16801 cccgagatgg caagggacga atcgacacca gacaggtgcg gacgatttcg gttttcccga
    16861 tatgcacaag acctaacaag ggacaaaacg gcgatttgcc cgcacaatgc ccgcaaaaaa
    16921 atacatctag aggatttgaa tttcggcaaa tttagcgaat caaaagttta tttcaatgaa
    16981 atcatatgat ttttttgaat aagcggattg atgggttttt gaaggaattt gttactggat
    17041 agccatcggg caagtttttt gcaaaatttg aatcggtcgg gtaaattttc aaaaaataat
    17101 tgacagcgga taagaaacgg cggataattc ccgccgtcga gttgcttgat gcagcttgat
    17161 ttttctcctc tatttctcct ttgtagactt ggcacacatt caactggatg tgtgcatttt
    17221 tttatctccg ctttttttga aatttaatta cttttattgt ttgaaattcc attctttaag
    17281 aaatttaaca agaaaaacgc ttgctttttg accggaaaag ctgcataatt cattccgtta
    17341 gctggttcga gtagtcagtt aatagtttct cctctatttc tcctttgtag acttggcaca
    17401 cattcaaccg gatgtgtgca tttttttatc tgaagcaaca agcctctgtg cgtgatgttg
    17461 ttatgtttca tttaggtgtc aaaccgcata gccagcctga aatattcaat ccaaatccaa
    17521 aaccggattt tctttgacct cctccatcac aacataactc ctactctccg aagcggcagg
    17581 caattgcaat aggatattgc ctagcatatc ccgataggca gacatatcgg gcaaacgtac
    17641 tttaatcaga tagtcgtatt cgcccgacac caagtggcat tccataattt gcggaatttt
    17701 cagcacttct tttttgaaat cttcgaaaat attgcc
//
LOCUS       DAWFJW010000035        17717 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFJW010000035 DAWFJW010000000
VERSION     DAWFJW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.035"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..309
                     /locus_tag="WGR19_000942"
     CDS             <1..309
                     /locus_tag="WGR19_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0798492.1"
                     /translation="ARVAYGHVRHSIDSTKKTTEVVTSTHGGADTKPTIYNGESTQNA
                     YHESHSIRRLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF
                     "
     gene            complement(1692..2504)
                     /locus_tag="WGR19_000943"
     CDS             complement(1692..2504)
                     /locus_tag="WGR19_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0798493.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGRAVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRTRAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            2589..3461
                     /locus_tag="WGR19_000944"
     CDS             2589..3461
                     /locus_tag="WGR19_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0798494.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            complement(3518..4672)
                     /locus_tag="WGR19_000945"
     CDS             complement(3518..4672)
                     /locus_tag="WGR19_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0798495.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(4717..5085)
                     /locus_tag="WGR19_000946"
     CDS             complement(4717..5085)
                     /locus_tag="WGR19_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0798496.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5259..5702
                     /locus_tag="WGR19_000947"
     CDS             5259..5702
                     /locus_tag="WGR19_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0798497.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            complement(5829..6449)
                     /locus_tag="WGR19_000948"
     CDS             complement(5829..6449)
                     /locus_tag="WGR19_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0798498.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(6557..7756)
                     /locus_tag="WGR19_000949"
     CDS             complement(6557..7756)
                     /locus_tag="WGR19_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0798499.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            complement(8170..9339)
                     /gene="prpF"
                     /locus_tag="WGR19_000950"
     CDS             complement(8170..9339)
                     /gene="prpF"
                     /locus_tag="WGR19_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0798500.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDSADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTTTKAVMSRSARVMMEGWVRVPEDCF"
     gene            complement(10142..12748)
                     /gene="acnD"
                     /locus_tag="WGR19_000951"
     CDS             complement(10142..12748)
                     /gene="acnD"
                     /locus_tag="WGR19_000951"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0798501.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(12839..13750)
                     /locus_tag="WGR19_000952"
     CDS             complement(12839..13750)
                     /locus_tag="WGR19_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0798502.1"
                     /translation="MKLRDLLMGIFLAVSAALLNATIGIFSKILMEQGLSVQHIAFLK
                     TLTGFVFISILLCRTGFTRQIADISRKKEAILPLLLKVAICAFFGIYTLFFFETTAYQ
                     YGNAANVVVVLMASAAVSALILDSILLDERICISSVVGVGLAVLGIAMISWTGEGSLG
                     LILNAALAGSGYGCFSVLIKKFGLNGGIYLTRILMFFGSIFLFIPSLEGIEDIHWQWS
                     FIPPLLALSLLPTILGFYCTTKALDYLSAAKVQVTELAEPLFAAVLAWLFLNEIPEGR
                     FFVGAILIIAGIVSINGLYRPLLKRIE"
     gene            complement(13737..14681)
                     /locus_tag="WGR19_000953"
     CDS             complement(13737..14681)
                     /locus_tag="WGR19_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEZ0798503.1"
                     /translation="MYHKKFDKEILLKIKELYRYDNWHAVLAILYDYLIIIFSIFLSE
                     QSYWFLPLTILLIGSRQRALATILHEASHSALTKNKKFGQILGTYFSGYLIFQSWNSY
                     CVSHVKNHHPKLGTDSDPDYKYYKDSGIFQTYSRIEFFWRFCVSQILCLKLFRNFKYL
                     IKNRLFSDITRTELFKLLLSQLILFVIGTYFVGYKFYVIYWLLPYLTAFQMITWFIEL
                     SEHYPMIQTAKNNLEASRNRFSHAIEHFFTGVHGENYHLIHHLFPAIPFWNLEKAHKI
                     LMKDFDYQEVNKDFGGIFFSKNNNAPMWKVLWSKYEAA"
     gene            complement(15007..16161)
                     /gene="prpC"
                     /locus_tag="WGR19_000954"
     CDS             complement(15007..16161)
                     /gene="prpC"
                     /locus_tag="WGR19_000954"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0798504.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSLLLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSG
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(16247..17122)
                     /gene="prpB"
                     /locus_tag="WGR19_000955"
     CDS             complement(16247..17122)
                     /gene="prpB"
                     /locus_tag="WGR19_000955"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0798505.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
BASE COUNT         4262 a         4795 c         4469 g         4191 t
ORIGIN      
        1 gcgcgcgtcg cctacggaca cgtcagacac agcatcgatt cgaccaaaaa aacaacagag
       61 gttgttacct ccacccatgg tggtgctgac acaaaaccta cgatttataa tggggaaagt
      121 acgcaaaacg cctatcacga aagccacagc atccgccgct tgggtcttgg tgtcgtcgcc
      181 ggtgtcggtt tcgacatcac gcccaagctg accctggaca ccggataccg ttaccacaac
      241 tggggacgct tggaaaacac ccgcttcaaa acccacgaag tctcattggg cgtgcgctac
      301 cgcttctgat tccccgatac cgatgccgtc tgaaccttca gacggcattt ttgatttacc
      361 tgccgtttac agacgcgggg cgggcgtgga aatacccgaa ccgtcattcc cgacaacacc
      421 gtaatcttga aacccgtcat tcccgcgcag gcgggaatcc agacccacga cgcggcggga
      481 atctatcgga aatgactgaa accccgagat tctagattcc cacttccgtg ggaatgacgg
      541 ttcagttgcg ttccgacaac accgcaatct cgaaattcgt cattcccgcg caggcgggaa
      601 tccagacccc tgacgcggcg ggaatctatc ggaaatgact gaaaccccga gattctagat
      661 tcccactttc gtgggaatga cgtggtgcag gtttccgtat ggatggattc gtcattcccg
      721 acaacacccc aatcttgaaa cccgtcattc ccgcacaggc gggaatccag acctgtccgc
      781 acagaaactt atcggataaa aacagttgcc caaaccccgc gttctagatt cccactttcg
      841 tgggaatgac gattcagttt cgttccgaca acaccgtaat ctcgaaattc gtcattcccg
      901 cgcaggcggg aatccagacc cctgacgcgg tgggaatcta tcggaaatga ctgaaacccc
      961 cgagattcta gattcccact tccgtgggaa tgacggttca gttgcgttcc gacaacaccg
     1021 caatcttgaa acccgtcatt cccgataaca ccgcaatctc gaaacccgtc attcccgcgc
     1081 aggcgggaat ccagaccccc gacgcggcgg gaatctatcg gaaatgactg aaacccccga
     1141 gattctagat tcccacttcc gtgggaatga cgtggtgcag gtttccgtat ggatggattc
     1201 gtcattcccg acaacaccgt aatctcgaaa tccgtcattc ccgcacaggc gggaatccag
     1261 acctccgacg cggcgggaat ctatcggaaa tgactgaaac cccgagattc tagattccca
     1321 cttccgtggg aatgacggtt cagttgcgct ccgacaacac cgtaatcttg aaacccgtca
     1381 tttccgacaa cgccgcaatc ttgaaattcg tcattcccga taacaccgca atctcgaaac
     1441 ccgtcattcc cgcgcaggcg ggaatccaga cccctgacgc ggcgggaatc tatcggaaat
     1501 gactgaaacc ccgagattct agattcccac ttccgtggga atgacggttc agttgcgtag
     1561 gactgggtcg tcgaaagggg cggattcgat ggattcgatg aaaacggtag aaatgtggga
     1621 ttgatggaaa tggcaggctg aagcccatcc tacagcccgc tgattccccg gcaccgatgc
     1681 cgtctgaacc ttcagacggc atccaaaacc cggtatttca gggcgggcag gcggttgcgg
     1741 acgctgttca ggcggttggc atcaatgtct gccgtaacga tgccttcgcc ttcgggcaat
     1801 acgtccaaca cgtcgcccca cggatcgaca atcatactgt gtccgaacgt gcgcctgccg
     1861 ttttcgtgca atccgccctg tgccgccgcc acaacataac attggttttc gacggcacgc
     1921 gtacgcaaaa gcagctccca gtgcgccttg cccgtcgtat gggtaaatgc cgccggaagc
     1981 atcaatacgt caaacggcag ctggcggcgg aaaaattcgg gaaagcggac atcgtaacaa
     2041 atacctgccg ccaccggcac gccctctgcc gacaagtgcg gcactgcccg ccctgcgctg
     2101 atggtatcgg cttcggcata gcgttcgccc aaaccggaaa agccgaagag gtgcattttg
     2161 tggtacagcc ccgtccgttt gccgtcgcgc ccgtacacca ataaagtatt catgaccttg
     2221 cccgcctcgg ggctttgcag cggcacggtt ccgccgaaca gcaccacgcc gcattctttc
     2281 gccgtttcgc tcaatgccgt ctgaaagcgt ccgccgccca aaggctcggc aagcgcgagt
     2341 ttgtcggtat cgtttgcgcc catcagcacc caatattcgg gcagcagcac ccaatccaca
     2401 ccctgctccg ccgcccgtgc gaccaggcgt ttcatggcag cgacgttggt ttccggcgac
     2461 acgcccgaca ccatctgcac ggcggcaact ctgattttgt ccatttcttc tcctttttcc
     2521 tgccgaactt gcggcatatt ccgccgatag gctaccatga cggtactttg cgaaaaccgt
     2581 ttacacaaat gcccattttg aaccatattg ccctcatcgg tgtagggctg atcggcggtt
     2641 cgttcgttct cgacctcaaa aggcagggac tcgtccgcac cgttaccggt atcgacaccg
     2701 accgcgacaa cctcgaacgt gcattggaac gcggcgtgat tgaccgggct tccgttgcca
     2761 tcgacgcgga cagcatcggc ggtgcggact tggtactgat tgccacgccc gtcgccaccg
     2821 ttcccgccat tttgaccgcg ctgcgccccg ttttgccgga acacacttgg atttccgatg
     2881 tcggcagcac caaatcttcg gtcatcgaag ccttccgccg ctgtctgccc gaccgtctgc
     2941 accactgcat cgccgcccac ccgattgccg gttcggacag aagcggtgcg caagccgcgc
     3001 agttcgggct gttccgccac agaaaactca tcatcacgcc acacggcggc gaacattcag
     3061 acggcattgc cttggtagaa gaactgtggc acgcggtcgg tgcggaaatt tatacgatgg
     3121 acgcgcaacg ccacgacgcg gttttcgccg ccgtctccca tatgccccac ctgaccgcct
     3181 tcgcctatgt ccaccagatt ctcgaccacc ccgacggaca ggaatatctg aaattcgccg
     3241 ccacgggctt tcgggacttc acccgcatcg cctccggcca tcccgccgtg tgggcggaca
     3301 tctgccttgc caacaaagac agcctgctgc aactggttca aggcttgggc aaacagttgg
     3361 acgttttggc aaacatcctg accaccgacg accgcgaagc cctgtaccgc tattttgaag
     3421 aagccaaaac cacacgcgac cgctggctgg acggcaactg accgccctgc cgtctgaaaa
     3481 gcaaaccgcc cagacccgaa atctgcggcg gtttgtctca accgggcctg ccgtccgaac
     3541 gcggacgaat cagccaagga atatacttcc acgcatacac caaaagcgcg agtgcaaaca
     3601 aaaccgaaga ggtgcgtatg ctgtgcgtgt aggcagtgcc ggaagaaaat acggcaacca
     3661 tacggacggc ggttgccgcc atcatcagcc aaaacgcaac gggaacggct ttgggcggcg
     3721 gataaatcgg attgcccgta tgaccgagcg cggtacgcgc catcatgccc aaagtcagca
     3781 cgccgatacc gccgaccccg atcagatgca cacccagatt gaggaaagcg ggtttgaaat
     3841 aagacgcgcc gaccgcaatc agccccaatc cggtaaacag atagccggca aacagaatcc
     3901 acagcatcgg ctctttcaac acgggtttat accaccagcg gtacacctgc acggtaaaaa
     3961 tcacacctgc cgcaaacgcg aaagccgccg acagccaagg catcacgccg tgcgccatca
     4021 gcatggcggt cagcatgggc agccacagcg aagcctgcgc cacccatttc ggactgggaa
     4081 tctgcggcac attcaaccgt ttggacgtaa aaaacgaaat aatccgcgta ccaatcagac
     4141 cgataaaacc cgacaccatc accaagcccg actgcaatcc gctcaagagt ccgcctaggt
     4201 tgccgttgtg cagctggacg tggaacgccg catgcgtgcc gcccaagacg aacagcgcga
     4261 acacggcaac atagttgcgt tgattctgtg aacggataac gggcaaagcc atgcacaccg
     4321 cgccgtacca gaaaaacagc gtaccgagta tgccgcttgc cgacgcaccc caacccggga
     4381 taaaggcggc aatccgcgca gccagccaaa agatagtcaa gccgaccaga acgccgcccc
     4441 gcgtgggcgg ctgccccgtc caagtggcga cggcggtcag caggaaggcg atgacgacca
     4501 gtccggcata accccaaatc atctcatgcg cgtgccaata gaaaccggac agctcgtgcg
     4561 ttcccgtgta gccgaaaccc cacagcaata cggacaatgc gccgtacaga gccgccagcg
     4621 aataaaatgg gcggaacgcc attgcccaga cggggtgctt ggtaaatttc atgtgtgttc
     4681 cttgtttatc attaatatat ttgccgcaaa acggaatcat gccggcaaaa acggttcggc
     4741 ggcgggaaac agctcgcgtt cttcaaagcg cgcgtggtcg cgcagggttg tggcaaaagc
     4801 ggtattccac gccgcgttac cgtattcggg gcttgccatc atctgccgca gtcgggcgtg
     4861 gtctttctcg aaacgttgtt tcaattcggg ggcgacattc tgccaaattg gggcaaactt
     4921 ggcttcttct tcgcgaaaat gggtttccaa ttcggcaaaa tgcggttcga gttcgtcccg
     4981 atgcctttct tccggcgtcc gcaacagacg cacgcacagg gaaagcgaat ggtggtggtc
     5041 acgcgaaagc ccgataaggg cgggatgtcg tttcaacggt ttcatgattt tgagttacaa
     5101 taacaaattc tcaaacggta gcgcggatgt ttcagacggc ataccgccgc agacaggctg
     5161 cattctaccg ccccgacgga cagggtttca accattaagg tattgtccgt gaaccttaaa
     5221 ccgtaccgat aagaataaca atccataccg agaaaatcat gtatttgaca caacatacgg
     5281 actacgggct gcgcgtcctt atctacactg ccatcaacga cgatgcgctg gtcaacatca
     5341 gtaccatcgc cgtaacctac ggcatttcca aaagccatct gatgaaggtc gttaccgcgc
     5401 tggtcaaagg cggcttcctc cacagtgtgc gcggaaaagg cggcggtctg cggcttgccg
     5461 caccgcccga ccgcatcaac atcggctcgg ttgtccgcca cctcgaaccg atgcagctgg
     5521 tcgagtgcat gggcgagaac aacgaatgcc tgatcacacc gtcctgccgg ctgacgggca
     5581 tactcggcgg cgcgatgaag tcgtttttca cgtatctgga cggtttcacg ctccaagacc
     5641 tgctcaacaa gccgacctac gacctgctct acgaaccgag aattccgatt gcggtgcagt
     5701 aatgcatcaa aatgccgttt caaacggata ttccgaccga gcgcgatacg cgccggcatt
     5761 tcggactgcc gccggcaagg cgcgcaatgc cgtctgaaca gccgatgctt cagacggcat
     5821 cggtttccct acaaaccgaa caccgccgta tcgccgcaac cttggcgcac caattccgtg
     5881 ccgtcggtca tatcgacgac ggtggtcggc tcggttccgc accagccgcc gtcaatcacc
     5941 aaatcgacgg cgtgttccaa acgctcgcgg atttcataag gatcggtcaa tggttcgccg
     6001 tcttcgggca gcatcagggt gcagcttaaa agcggctcgc ccaattcccc cagcagggct
     6061 tgtgcaatgg cattatcggg aatacgcagc ccgatggttt tgcgtttcgg gtgcagcgtg
     6121 cgcgccggca catccttcgt cgcctgtaaa ataaaagtat aaggcccggg tgtggcggct
     6181 ttaagctgac gaaactgtac gttgtcgact ttggcgtatg tgcccaactc gctcaaatcg
     6241 gcgcacatca gggtcaggtg gtgtttcaaa tcgattttgc ggatggagag tatgcgttcc
     6301 atcgccgcct tatcgccgag tttgcagccc aaggcataac aggaatcggt cggataaacg
     6361 accacgccgc ctttattgac gatttcgacc gcctgcttga tgaggcgttc ttgggggttg
     6421 tcgggatgaa cggcgaaaaa ctgtgccatg gtttgtttcc ttatcgtcat gttttccgta
     6481 ttgtacgccc aaacgccaaa caaaatgccg tctgaaaatt tcagacggca tttttgcgga
     6541 ctcgtccctt ttttggctac aagatgccgg caagttcggc agtgtcgcag gcaatcatca
     6601 gttcttcatt ggtcgggaca accaaaacag ccggagaaga atcggtcggg ctgataatgc
     6661 ccgaattgcc gtagcgtttt tccatattgg ctttggtgtc gatgtgcaga cccaagaaat
     6721 caagatagga aacggttttg gcacggatat tacgcgagtt ttcgccgata ccgccggtga
     6781 acacgagtgc gtcaacgccg ccgcagccca cagccatcga agcgatgtat ttggcgaggc
     6841 ggtaggtcat gacttcgagg gcgaggcgcg cgccttcgtg gccttcgtcg gcggcgattt
     6901 cgagggtgcg gcagtcgttg gaaagttcgg aaataccgag caaacctgat tttttgttca
     6961 gcatttcatc cacttgggca acatccatcc cggcgtggga agtcagatag ctgtatacgc
     7021 ccggatcgat gtcgccgcaa cgtgtaccca ttaccaaacc ttcgatcggc gtgaaaccca
     7081 tactggtatc gacggatttg ccgtttttga tggcggtaat ggatgcgccg ttgcctaagt
     7141 gggcaataat catgcggatg tcttccagag gtttgcccaa gatgcgtgcg gcttcagggg
     7201 caacgtaacg catactggtg ccgtggaaac cgtagcggcg gaaagcgtat tttttacgca
     7261 actcgcgcgg cacggcataa gtgtaggcac gctccggcat ggtttggtgg aacgaagtat
     7321 ccatcacgcc gacattgggc agaccgggga aatgttcctg tgcggcaagg atgccgctga
     7381 tgttggcggg gttgtgcagc ggcgcaagcg gaatgcaggc attgagttcg tccattacgg
     7441 cctggtcgat caaaacagac tcgctgtatt tttcgccgcc gtgggcgatg cggtggccga
     7501 tggctttgat gcggtcgtgc agaccgtgtt tttccagttc gttcaacagc atacccaccg
     7561 cgccggcatg gcagttgcgg ccgctcaggg gaacttggcg tttgttgccg tctttgttga
     7621 aggtaatgac ggcttcgggc gtggtcaggc gttcgccgag gcagcttagg acgacgctgc
     7681 cgcttttgcg gtcgataacg gcacctttga gcgatgaact gccgcagttc agaacaagaa
     7741 tgagttggtc ggacatgatg tttctccttg tagtttgtgg ttgttttttg gtgtggggtc
     7801 gtctgaaaaa ggatgggcgt tgggcggagt gtgtgtcaga cggcctcttt gggcaaagca
     7861 tatgccgcat ttcctatcgt tattccgtcc ccgcctacgc aaggacaggc tgtggcggga
     7921 atccagaatg tagaactcac gaaacctatt ttcccctgcc aatcttccat attgacaggt
     7981 ctggattccc gcctgcgcgg gaatgacggt aatacgggaa gctgtctttt atcggggttt
     8041 ccgtgttctt ccttgtgatt tgccgccttc cgccacaggg caagggaaag gaatggtcag
     8101 tgatttgatg tttacttgtt tttcagacga cctttttatg gctcgcgagc aaacccacgc
     8161 tacgtcgatt taaaaacaat cctcaggcac cctgacccaa ccttccatca tcacgcgtgc
     8221 gctgcggctc ataaccgctt tggtggtcgt ccattgtccg tcctgacatt cggcggctgc
     8281 accgacacgc aatgtgccag aaggatgccc gaagcgcact tctttacgcg ttccgccgcc
     8341 tgcggcaagg ttgaccagcg taccgggcac ggcggcggcg gtcgcaatgg caacagaggc
     8401 ggtacccatc atcgcgtggt gcagtttgcc catgctcagg gcgcgtacca gcaaatcgat
     8461 gtcggcggcg ttcacggttt tgccactgga ggcggtgtaa tcggcggcgg gcgcgacgaa
     8521 ggcgactttc ggcgtgtgcg cgcgggcggc agcttcggat acgtcgctga tcagacccat
     8581 tttcagcgca ccgtaagcgc ggattttctc gaatttttcc aaagccgcgg catcgttgtt
     8641 gatgtcgtct tgcaactctt tgcccgtgta gcccaagtcg gcggcattca ggaaaacggt
     8701 cggaatgccc gcgttgatga gcgtggcttt caaacggcct atattcggca catcaatttc
     8761 atcgaccaaa ttgccggttg ggaacatact gccttcgccg tcggctgaat caagaaattc
     8821 gatttgtact tcggctgccg ggaacgttac gccgtcgagc tcaaaatcgc ctgtttccaa
     8881 aactgcgccg ttttgcatcg gtacatgggc aataatggtt ttgccgatgt ttttctgcca
     8941 gattttgacc gtgcagatgc cgtctgaagg aatcttgcct ttatcgacca agccctgttc
     9001 gatggagaat gcgcccacgg cggcggtgag gttgccgcag ttgccgctcc aatcgacaaa
     9061 aggtttgtcg atggaaactt gcccgaaaag gtaatcgaca tcgtgatcgg cgcgttcgga
     9121 cttgtccaaa atcacggctt tgctggtgga cgaactggcg ttgcccaaac cgtctatctg
     9181 cttgccgtag ggatccgggc tgccgagtac gcgcaagagg attttgtcgc gtgcgcttcc
     9241 cgcttcccgc gccgcctcgg gcaggtcgga acgtttgaaa aacacgcctt ttgatgtacc
     9301 gccacggtag taaacggcgg gaattttaat ttgcggcatt tctagattct ccttatgtag
     9361 cgtgggctct gcccacgatt tttatagtgg attaggctgc cactccgacc aaagccgttt
     9421 gattccgcaa actgtcgggg ttcgccccaa tctacggcta ctgtgttgtg atacggcaga
     9481 ttattaaacc ccgtcattcc cgcgcaggcg ggaatccaga tttatccgca cagaaactca
     9541 tcggataaaa aggtttcctc aattccactt tctggattcc cgcctgcgcg ggaatgacga
     9601 tttacagtat agtggattaa atttatagtg gattaacaaa aaccagtaca gcgttggctc
     9661 gccttagctc aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt
     9721 actatctgta ctgtctgcgg cttcgccgcc ttgtcccgat ttttgttaat cctctatacc
     9781 atgtcaattc agcctttatt tcgcgggcaa agcccacgct acacccactt tccagaagta
     9841 cattaggctg ccactccgac aaagccgttt gattccgcaa actgtcgggg ttcgccacaa
     9901 tctacggcta ctgtgttgtg atacggcaga ttattaaacc ccgtcattcc cgcgcaggcg
     9961 ggaatccaga tttgtccgca cagaaactca tcggataaat aaggtttcct caattccact
    10021 ttctggattc ccgcctgcgc gggaatgacg atttacagta gatgcctgcc atatcgagaa
    10081 ttacgtgaat gagcaaaatg ttgaacccga cccacgctac gtcttgcttt tcagacgacc
    10141 tctaagccgc gttcccttcc aaaaaatcct gtgcaaaccg ttgcaacacg ccgccggctt
    10201 catataccaa tacttcttct gcagtatcga ggcggcaggt aacgggaact tcaacggttt
    10261 cgccgttttt acggtgaatc acgagggtca ggtcgcagcg cggtgtgcgt tcgccgacca
    10321 cgtcgtaggt ttccgtaccg tccagttgca gggtatggcg gttggtgtcg ggtttgaact
    10381 gcagcggcaa cacgcccatg ccgataaggt tggtgcggtg gatacgctcg aagccttcgg
    10441 caacaatcgc ttctacgccg gcgaggcgta cgccttttgc agcccagtcg cggcttgagc
    10501 cttgaccata gtccgcaccg gcaatgatga tgagcggctg tttgcggttc atataggttt
    10561 cgatggcttc ccacatgcgc atggtttcgc cttcgggttc gacgcgggcg aacgagcctt
    10621 ggcgcacgct gccgtcttcg tttttcacca tttcgttaaa cagtttcgga ttggcgaagg
    10681 tagcgcgttg ggcggtcaag tggtcgccac ggtgggttgc gtaagagttg aagtcttctt
    10741 caggcaaacc cattttcgcc aaatactcgc ctgcggcact gacggccaaa atcgcattgg
    10801 acggcgagag gtggtcggtg gtgatgttgt cgggcaaaat cgccagcgga cgcatacctc
    10861 ttaatgtgcg ttcccctgcc agcgcgcctt cccagtaagg cggacggcgg atgtaggtgg
    10921 acatcggacg ccaatcgtac agcggactgg gtgctttttg cgctgtgccg gtgtcgaaca
    10981 tcggtacata cacatcgcgg aactgctgcg gtttcacata ttcggcaacg acggcatcga
    11041 tttcttcatc ggcaggccaa atgtctttca ggcggatttc cttgccgtct gcaacgccga
    11101 gtacgtcgtt ttcaatatcg aaacggatac tgcctgccag cgcgtaggca acgaccaacg
    11161 gaggcgaagc gaggaaagcc tgtttcgcat acggatggac acggccgtcg aagttgcggt
    11221 tgcctgataa tacggcggtg gcgtacaaat cgcggtcgat gatttctttc tggattttcg
    11281 gatccagcgc gccactcatg ccgttgcagg tggtgcaggc gaaggcgacg ataccgaagc
    11341 cgagtttttc catttcgggc agcaggcctg cttctttcaa ataaatttcc gccactttcg
    11401 aaccgggggc aaacgaggtt ttgacccacg gtttgcgttt cagcccgaag cggttggcgt
    11461 tgcgcgccaa gagcgcggcg gcaacgacgt tgcgcgggtt ggaagtgttg gtgcaactgg
    11521 taatcgcggc gatgatgacc gcgccgtcgg gcatttggcc gtctgaaggc tcttcgtaag
    11581 gcttcgccag ccctttcgcc gccaaatcgg cggtcgcaaa acgggcatgc gggttacttg
    11641 gacctgccat attgcgcgtt acgctgctca aatcaaattt caaaacgcga ggataaacgg
    11701 cggttttcaa ggcatctgcc cacaagcctg cggttttggc gtaggtttcc accaatttca
    11761 cctgcgcgtc gtcgcgtccg gtcagtttca aataatcaat ggtttgctca tcaatagcga
    11821 acatcgcggc agtcgcgccg aactccggcg tcatgttgga aatggtcgcg cggtcgccga
    11881 tagacaggct tctcgcgccc tcgccgaaga attcgacaaa cgccccgacc acgcgttctt
    11941 tgcgcagaaa ctcggtcagt gccaacacaa tatccgtcgc cgtaatgccc gcctgccgtt
    12001 tgccgttcag ctcaacgccg acaatatcgg gcaggcgcat catggacgcg cgtcccagca
    12061 ttacggtttc cgcttccaat ccgcccacgc ccacggaaat cacgcccagc gcatcgacgt
    12121 gcggcgtgtg cgaatccgtg ccgacgcagg tatcggggaa cgccacgccg tttttgactt
    12181 ggacgacggg cgacattttt tccagattga tttggtgcat gatgccgttg cccgccggaa
    12241 tcacgtccac attttcaaag gcggttttcg tccagttgat gaagtggaaa cggtcttcgt
    12301 tacgtctgtc ttcgatttgg cggtttttgc ggaaggcatc ggggtcgtag ccgccgcatt
    12361 caacggccaa agagtggtcg acgatgagct gggtttgcac caccggattc actttggcag
    12421 gatcgccgcc tttttcggca atcgcatcgc gcagacctgc caaatccacc aacgcggtct
    12481 gccccagaat atcgtggcac accacccgcg ccggatacca aggaaagtcg atttcctgtt
    12541 ttccctcaat cagctgaccc agccagcttt gcagcgtcgg caaatcgact ttgtccgcgc
    12601 ggttgaccaa attctccgcc aaaatgcggc tcgtgtaagg cagcttgtcg taagagccgg
    12661 gcttgatgtc ctcacacgcc gcacgcgcgt cgtagtattc caaatccgta ccgggcagcg
    12721 gtttgcggta acgttggttg gcagccatgt cggttctcct aaatctatta tttttttggt
    12781 tttggggttt cagacgacgt ttgttgaaga ggtcgtctga aattttggga aataaaaatt
    12841 attcaattcg cttcaacaat ggtcgataca gcccattgat agacacaata ccggcaataa
    12901 tcagaatggc gccgacaaag aagcgtcctt ccggtatttc attcaaaaac aaccaagcca
    12961 gtacggcagc aaacaatggc tcggccaatt cagttacctg taccttcgca gcactcaaat
    13021 aatccaatgc tttagttgta caataaaatc ctaaaatcgt cggcaataaa gacaatgcca
    13081 agagtggcgg aataaaagac cattgccaat gtatatcctc aataccttcc aatgaaggga
    13141 taaacaaaaa aatacttcca aaaaacatca atatccgtgt caaataaata ccgccgttta
    13201 ggccgaattt cttaatcaaa acggaaaaac aaccgtagcc cgagcccgcc agtgcggcat
    13261 tcagaatcaa ccctaaactt ccttctccag tccaagaaat cattgcgatc cccaatactg
    13321 ccaaacccac accgacgact gaagaaatgc aaatacgttc atctaacagt atgctgtcca
    13381 atatcaaggc agatacggca gccgatgcca ttaatacaac tactacattc gcagcattgc
    13441 catattgata agctgtggtt tcaaagaaaa acaacgtata aattccgaaa aaagcacaaa
    13501 ttgctacttt taacagcaac ggcaaaattg cctctttctt tcttgaaata tccgcaatct
    13561 gtctggtaaa accggtacgg caaagcaaaa tgctgataaa cacgaaacct gtcaaagttt
    13621 tcaaaaatgc aatatgctga acagacaagc cctgctccat caatatcttg ctgaatatgc
    13681 cgatggttgc attcagaagg gccgcagaaa ctgccaagaa tattcccatc agtaaatcac
    13741 gcagcttcat atttactcca taatactttc cacataggtg cattattgtt tttcgaaaag
    13801 aaaatccctc caaaatcttt atttacctct tgataatcaa aatctttcat taatatctta
    13861 tgtgcttttt ccaaattcca aaatggaatg gctggaaata aatgatgtat tagatgataa
    13921 ttttctccat gtacccccgt gaaaaaatgc tctattgcat gactaaacct atttcttgac
    13981 gcttctaaat tatttttggc agtttgtatc attggataat gctcggacaa ctcaataaac
    14041 caagttatca tctggaatgc agttaaatat ggtaataacc aataaattac ataaaattta
    14101 taccctacaa aatatgtacc gataacaaat agaattaatt gagaaagaag tagcttaaac
    14161 aactctgttc ttgtaatatc cgaaaataat ctatttttaa ttaaatattt aaaattccta
    14221 aataatttca aacataaaat ttgagagaca caaaatctcc agaaaaattc aattcttgaa
    14281 tatgtctgaa aaatacctga gtctttataa tacttataat caggatccga atcagtccct
    14341 aactttggat gatgattctt cacatgagaa acacaataac tattccaact ttgaaaaatt
    14401 aaatatccag aaaaatatgt acctaaaatt tgtccaaact tcttattttt agttaaggca
    14461 gaatgagagg cttcatgtaa aattgttgcc aacgcccttt gcctgcttcc tattaataaa
    14521 atcgttaaag ggagaaacca atatgactgc tcagataaaa atatagaaaa aattattatt
    14581 aaataatcat ataatatagc aagtacagca tgccaattat cgtatctata taattcttta
    14641 atttttaaaa gaatttcttt atcaaatttc ttatggtaca tatcccacaa ttctccgtat
    14701 tagttataat atcggcttca tctccattat gctgacgaac attctttcat agcatatttt
    14761 ggcagaaccg tgaaaaaagt tgccgcgctg cctccgccat tcccgccgcg acctgtcctc
    14821 gcttaggcgg gggtgggaat gacggcagac gttgcggctg gtttttcaga caaccttctc
    14881 tctcgcatct ttcaacaggc cgtctgaaaa gcggaaccgt aggcgggttt cagttttccg
    14941 caggcaaagc ccgcgctaca cgtcgtctga aatccgattg gtggtctcct acggttgtgt
    15001 tccgtctcag cgttcttcaa tctccacaaa cgccaaatct tcagggcctg tgtagttcgc
    15061 gctcggacgg atgattttgc cgtctttgcg ttgctcgaga acgtgtgcgc tccaaccggt
    15121 tgtacgggaa attacgaaca gcggtgtgaa catagcggtc ggtacgccca atttttggta
    15181 ggaaacggca gagaaccagt ccagattcgg gaacattttt ttctcttccc acatcacgct
    15241 ttccaagcgt tcggcaatgt caaagaggcg catatcgccg gtttctttgc tcaaaccgcg
    15301 tgccacttct ttaatgacaa cgttgcgggg gtcggaaatg gtgtacaccg gatgaccgaa
    15361 accgatcacg atttctttgc ggccgatgcg ttcgcggatg tcggcttcag cttcgtcggc
    15421 attgcggtag cgtttttgaa tatcgtaagc cacttcgttc gcgccgccgt gtttcggacc
    15481 tttcaacgcg ccgattgcac cggtaatgcc ggagtacata tcagagcctg taccggcaat
    15541 cacgcgggcg gtaaaggtag aagcgttgaa ctcgtgttcc gcatacagaa tcagtgaaac
    15601 gtgcatggct ttaatgtgtg attcgcttgg gcgtttgccg tgcaacagtt gcaggaaatg
    15661 accgccgatg gtctcttcgt cgctttcaac ttcaatgcgt ttgccgttgt gcgaatattg
    15721 ataccagtac agcaggaggc tgccgaggct ggcaatcagt ttgtcggcga tgtcgcgcgc
    15781 ttcactttcc ggatggcttt cacgttcggg atgaacgcag cccagcatgg atacgccggt
    15841 acgcattacg tccatcggat gggtatgtgc aggcaggctt tccaaaactt taatcacacg
    15901 gataggcagg ccgcgcatgg atttgagctt ggttttataa gcggccagct cgaatttgtt
    15961 gggcagatga ccgtgaatca gcaggtgggc gacttcttca aactcgcatt tttgtgccaa
    16021 atccaagatg tcgtaaccgc gatagctcaa atcgttgccg gtacggccaa cggtacacaa
    16081 agcggtatta ccggccgcaa cgcctgaaag cgcaacggat ttcttaggtt tgaaggtcgg
    16141 ggtttgagta gtttcagtca tggtatttct cctttgtgtt tttatgggtt tcgggttttc
    16201 agacgaccga tgcggatttg ttgaaagacc gtctgaaagc ggtaaatcat ttttgaaaca
    16261 atttatccag tttttgctcg aaggcatgat agttcagatg ctcgtacagc tcggcacggg
    16321 tttgcatact gtccaccacc gccgcctgag tgccatcgcg cataatcgct tcgtaaacat
    16381 tcagagcggc tttgcttgct gcacggaacg atgacagcgg atacagcacc agcgacacgc
    16441 cgttttcagc cagctcgctt tgggtataaa gcggagtgga accaaactcg gtaatgttcg
    16501 ccaacacggg cactttcacc gcatctgcaa attggcggta catgttcaaa tcggtcatgg
    16561 cttcagggaa aatcatgtcc gcaccggctt cgacacaagc ttgggcgcgt tcgatagcgg
    16621 catccaaacc ttctaccgcc agcgcatcgg tacgcgccat aatcacgaag ttctcatcaa
    16681 cgcgcgcatc tacggcagct ttgatacggt cgaccatttc atctttagat acaatggctt
    16741 tgttcggacg gtggccgcag cgtttttgcg ctacctgatc ttcgatgtga accgctgcaa
    16801 caccggcgcg ttcaaagttg cgaatggtac gggcaatatt gaatgcaccg ccccaaccca
    16861 catcgatgtc caccagcaga ggcgtatcca cgttgtccgt aatgcgtcgt gcgtcgatca
    16921 gcacatcttc cattgtggta atgcccaaat cagggatacc gcaagaacag gctgccacgc
    16981 cgccgccgga cagatagatg gctttgaaac cgctttgggt ggccaatcgt gcaaaataag
    17041 cattgacgca accggcgacg gcaagcggat tcgattcttt cacggcttgg cggaaacgtg
    17101 ctccggcaga gtgttgactc atcatatttc tcctttatag actttttttc agtatggaca
    17161 ggcttccatc acattcggac ggcaaaacac atccatccga cgcatcgggc atcccaatcc
    17221 aatcattaaa aatatatggg aaaaattatc ttattgatat ttaaaacgaa tcaaagaaaa
    17281 acagcagacc gttcggaatt atgcggcaaa accgcagaca agaagaaaac aaggggatta
    17341 ttcagaaaag gggaaaacat ctgaattggt ttcatagtaa tgttcctttg tagtgttatg
    17401 tagttttatt tttcgataat ctaaaacaca agactgaaaa tgtcaaacat ttttaaggag
    17461 actcattaca taattttaat attttatttt aattctatga ttaaactaga ttaattttga
    17521 attatttgtt tcaataaaat tgtcaggaaa tcaagaaaaa ttttcaataa tcaaaatcct
    17581 gaaaacttat aaaatatttt atacataaaa acaatgtatt atttttaagt aatcttatgt
    17641 agtttgttat ctatttgatt tttatattat ttaattaaat ttaaatatat agtggattga
    17701 atttaaatca ggacaag
//
LOCUS       DAWFJW010000036        16937 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFJW010000036 DAWFJW010000000
VERSION     DAWFJW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.036"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            563..6010
                     /gene="iga"
                     /locus_tag="WGR19_000956"
     CDS             563..6010
                     /gene="iga"
                     /locus_tag="WGR19_000956"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0798506.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRVGAGRQLVYEKGAYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNTHYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADEIKQRDNAGTIKG
                     NGEHHWNITFGTNSKIGSTAVRLAGNERDANNGQNVTFEDNGTLVLDQNINQGAGGLF
                     FKGDYTVKGINNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQ
                     GQLKVGDGTVILNQKADSNQKVQAFSQVGIVSGRGTLVLNSPDQINPNNLYFGFRGGR
                     LDANGNDLTFEHIRNVDEGARIVNHNTDRASTITLTGKSLITDPKTISIHYIQNNDDD
                     DAGYYYYRPRKPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQE
                     EAKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGK
                     ISVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVN
                     QSASFSSGRNVSNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKAL
                     NSFGATQINGNVNLNQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNL
                     SLADSHIHLNNASDAQSANQYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGH
                     YQLHVQDKTGEPNQEGLNLFDASSVQDRSHLSVSLANNHVDLGALRYTIKTENGITRL
                     YNPYAENRRQVKPAPSPATNTASQAQTDSAQIVKPQNIVVAPPSPQANQAEEAKRQQA
                     KAEQVKRQQAEAERKSAKLAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREA
                     AELSAKQKVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEE
                     ERRQTAQSQSQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVRRQQE
                     ERKAAELLAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQK
                     AAAEAQALAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQ
                     EARQTAELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRK
                     RRAAPQDYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIP
                     LDALEDENVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQP
                     QDNTELHEKVETVSLQPRAAQPRAQAAAQADAVSTNTNSALSDAMASTQSILLDTGAS
                     LTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQI
                     GGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKA
                     NFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLT
                     AGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLY
                     RNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(6188..7390)
                     /gene="trpB"
                     /locus_tag="WGR19_000957"
     CDS             complement(6188..7390)
                     /gene="trpB"
                     /locus_tag="WGR19_000957"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0798507.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7473..8162)
                     /locus_tag="WGR19_000958"
     CDS             complement(7473..8162)
                     /locus_tag="WGR19_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0798508.1"
                     /translation="MLFESLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            8374..9153
                     /gene="rsmA"
                     /locus_tag="WGR19_000959"
     CDS             8374..9153
                     /gene="rsmA"
                     /locus_tag="WGR19_000959"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0798509.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELATDEDLQAAGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            9326..10054
                     /locus_tag="WGR19_000960"
     CDS             9326..10054
                     /locus_tag="WGR19_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0798510.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQGFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            10127..10486
                     /locus_tag="WGR19_000961"
     CDS             10127..10486
                     /locus_tag="WGR19_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798511.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDTEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            10491..10937
                     /locus_tag="WGR19_000962"
     CDS             10491..10937
                     /locus_tag="WGR19_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0798512.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            10969..12243
                     /gene="folC"
                     /locus_tag="WGR19_000963"
     CDS             10969..12243
                     /gene="folC"
                     /locus_tag="WGR19_000963"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0798513.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKVGTLTSPHLLRYNERIAVNAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGTYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            12256..13254
                     /gene="ftsN"
                     /locus_tag="WGR19_000964"
     CDS             12256..13254
                     /gene="ftsN"
                     /locus_tag="WGR19_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0798514.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            13247..13744
                     /locus_tag="WGR19_000965"
     CDS             13247..13744
                     /locus_tag="WGR19_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0798515.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFILAYLIQKILRSLLTQAVSSVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHADDSDG
                     ALEDD"
     gene            14056..15600
                     /gene="purF"
                     /locus_tag="WGR19_000966"
     CDS             14056..15600
                     /gene="purF"
                     /locus_tag="WGR19_000966"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0798516.1"
                     /translation="MCGVLGLVSHAPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMVAFRDPYGIRPLALGSQTDESGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVYLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            15700..16191
                     /gene="greB"
                     /locus_tag="WGR19_000967"
     CDS             15700..16191
                     /gene="greB"
                     /locus_tag="WGR19_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0798517.1"
                     /translation="MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            16250..16876
                     /locus_tag="WGR19_000968"
     CDS             16250..16876
                     /locus_tag="WGR19_000968"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0798518.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         4756 a         4570 c         4161 g         3450 t
ORIGIN      
        1 aaaccaaaat cagaaaccta aaatcccgtc attcccacga aagtgggaat ccagaatctc
       61 agactttcag ataatctttg aatattgctg ttgttctaag gtctagattc ccgcctgcgc
      121 gggaatgacg aatccatccg tacggaaacc tgcaccgcgt cattcccacg aacccacatc
      181 ccgtcattac cacgaaccca catcccgtca ttaccacgaa agcgggaatc taggacgcag
      241 ggttaagaaa acctacatcc cgtcattccc tcaaaaacag aaaaccaaaa tcagaaacct
      301 aaaatcccgt cattcccgca caagcgggaa tccaaaaaca aaaagccgca gaaatttaac
      361 ggaagaaccg aaccccgaaa atcggcttcc caccttcgcc tgaataacag aacaaaggaa
      421 gcggaaataa aaacatcggg attctttttt taaattggca ataaaacacc aaaatgaata
      481 attcacacaa caaaaaaata gtcaacaccg ccaaatcggc gtaaaataca agacattatc
      541 acccttaaaa cggtaaaacc ttatgaaaac caaacgtttt aaaattaacg ccatatcctt
      601 atccatcttt cttgcctatg cccttacgcc atactcagaa gcggcattgg tcagagacga
      661 tgtcgattat caaatattcc gtgactttgc agaaaacaaa ggcaaatttt ttgtcggcgc
      721 aaccgattta tcagtgaaaa acaaacaagg tcaaaacatc ggcaacgcat tatccaacgt
      781 accgatgatt gattttagcg ttgcagatgt caacaaacgc atagcaacgg tagtcgatcc
      841 ccaatatgct gtcagcgtca aacacgccaa agcagaagtc catacttttt attacggtca
      901 atacaacggt cataatgatg tagccgacaa agaaaatgaa taccgcgttg tcgaacaaaa
      961 taactatgaa ccacataaag cttggagtgc gagcaactta ggccgtctcg aagattataa
     1021 tatggcgcgt tttaacaagt ttgtaaccga agttgcgccg attgcaccaa ctgatgccgg
     1081 cggcggattg gatacctaca aagataaaaa ccgtttttcc tcttttgtca gagttggcgc
     1141 aggcaggcaa ttagtttatg aaaaaggggc ttatcatcaa gaaggaaatg aaaaaggcta
     1201 cgatttgcgc gatctttcac aagcctatcg ttatgccatt gcaggtacac cttataaaga
     1261 tattaatatt gaccaaacaa tgaataccga aggtttgatt ggctttggta atcacaatac
     1321 acactattca gcagaagaac ttaaacaagc actctcgcaa gatgcgttaa caaattacgg
     1381 cgtattgggc gatagcggct ctccgttatt tgcttttgac aaacaaaaaa atcaatgggt
     1441 ctttttggga acttatgatt attgggcagg ttacggaaaa aaatcttggc aagaatggaa
     1501 tatctataaa aaagaatttg cagatgaaat caaacaacgc gataacgccg gcaccatcaa
     1561 aggtaatgga gaacatcatt ggaacattac ttttggcaca aacagcaaga ttggttctac
     1621 agcggtaagg cttgccggca atgaaaggga tgcaaacaac ggacagaatg ttacctttga
     1681 agacaacggc actttggtat tggatcaaaa catcaaccaa ggcgcgggcg gtctgttttt
     1741 caaaggcgat tacacagtca aaggtataaa taatgacatc acttggctag gtgcggggat
     1801 tgatgttgcc gacggcaaaa aagtcgtttg gcaggtcaaa aatccgaatg gcgacagatt
     1861 ggcaaaaatc ggcaaaggca cattagaaat aaacggcaca ggcgttaacc aagggcaatt
     1921 aaaagtcggc gacggtacgg ttattctgaa tcaaaaagcc gattcaaacc aaaaagtcca
     1981 ggctttctcc caagtcggca ttgtcagcgg acgcggtaca ttggtgctga acagtccgga
     2041 ccagattaac ccgaataatc tgtatttcgg tttccgtggc ggtcgtttgg atgccaatgg
     2101 caatgacttg acttttgaac acatccgcaa cgtggatgaa ggcgcgcgca ttgtcaacca
     2161 caacacagac cgcgcctcca caatcacgct aacgggtaaa tctttgatta ccgatcccaa
     2221 aaccatctct attcattata ttcaaaataa tgatgatgac gatgctggtt attactatta
     2281 ccgccctagg aaaccgattc cgcaaggcaa agatctttat tacaaaaact atcgttatta
     2341 cgccctaaaa tccggcggca gcgtgaacgc accgatgccc gaaaacggac aaacggaaaa
     2401 taacgactgg attttaatgg gcagcacaca agaagaagcc aagaaaaacg cgatgaacca
     2461 caaaaacaat cagcgtatta gcggttttag cggtttcttt ggcgaagaaa acggaaaagg
     2521 acataacggt gcattaaacc ttaatttcaa cggcaaaagc gcgcaaaacc gtttcttgtt
     2581 aacaggtggc actaatttaa acggaaaaat aagcgtaact caaggcaatg tcttgttatc
     2641 aggtcgtcca acaccacacg caagagattt tgtgaacaaa tcttcagccc gaaaagacgc
     2701 acatttctcc aaaaacaatg aagtcgtatt tgaagacgac tggataaacc gcacattcaa
     2761 agccacagaa attgcggtta atcaatccgc atcattctct tccggcagaa atgtatccaa
     2821 cattaccgcc aacatcaccg caacagacaa cgccaaagta aatttgggtt acaaaaacgg
     2881 cgatgaggtt tgcgtgcgct cggactatac cggttatgtt acctgcaaca caggcaactt
     2941 atccgataag gctttaaaca gctttggagc gacacagatt aacggcaatg tgaatctgaa
     3001 ccagaatgcg gcattggtct tgggcaaggc tgcattatgg gggcaaattc aagggcaagg
     3061 aaacagcagc gtcagcctaa atcaacatag caaatggcat ttgaccggcg acagccaagt
     3121 acacaatctg tcattggcgg atagccatat tcatttgaac aacgcttccg atgcgcaaag
     3181 tgcaaatcaa taccacacgc tcaaaatcaa tcatttatcc ggtaacgggc attttcatta
     3241 tctgacgcac ctggcgaaaa atcttgggga taaagtgctt gtgaaggaat ccgcatccgg
     3301 tcattatcag ctccatgttc aagataaaac aggcgaaccc aatcaggaag ggctgaatct
     3361 ctttgatgca tcatccgtac aagaccgttc gcacctttct gtttccttgg cgaataacca
     3421 tgtagattta ggcgcattgc gttatacaat caaaacagaa aacggtatta cccgattgta
     3481 caatccttat gccgagaacc gccgccaagt caaaccggct ccgtctcctg ccacaaacac
     3541 ggcttctcaa gcacaaacgg acagtgcaca aattgtcaag cctcaaaata tcgtcgtcgc
     3601 accgcctagc ccgcaggcaa atcaagccga agaagcaaaa cgccagcaag caaaagcgga
     3661 gcaagtgaag cgtcagcaag cagaagcaga gcgtaaatcg gcaaagttag caaaacaaaa
     3721 agcagaagcc gaacgcgaag caagagagct ggcgacccgc caaaaagcag aacaagaacg
     3781 cagcagcgcc gaacttgcac gtcgacatga aaaagaacgc gaagcggcag agttgtcggc
     3841 aaaacaaaaa gtcgaagccg aacgtgaggc tcaagcattg gcggtacgcc gaaaagccga
     3901 agccgaagag gcaaaacgcc aagctgcaga gcttgcacgc cggcatgaaa aagaacgcga
     3961 agcggcagag ttgtcggcaa aacaaagggt cggggaagag gaacgccgtc aaacagccca
     4021 atcccagtcg caacgccgca aacgccgtgc cgcaccgcag gattatatgg cagcttccca
     4081 agaccgtcct aaacgccgcg gacaccgttc ggtacagcaa aacaatgtgg aaattgccca
     4141 agcccaagca gaacttgtgc gccgacagca ggaagaacgc aaagcggcag agttgttggc
     4201 aaaacaaagg gcagaagccg aacgtgaggc tcaagcattg gctgcgcgcc gaaaagcaga
     4261 agccgaagag gcaaaacgcc aagctgccga acttgcacat cgccaggaag cagaacgcaa
     4321 agcggcagaa ttgtcggcaa accaaaaagc cgcagctgaa gcccaagcat tggcggcacg
     4381 ccaacaaaaa gcccttgcgc gtcaacagga agaagcacga aaagctgcag agttagccgt
     4441 caaacaaaaa gcggaaaccg aacgcaaaac cgccgaactt gccaagcaaa gggcagcagc
     4501 cgaagcagca aaacgccagc aagaagctcg ccagaccgcc gaacttgcac gtcgtcaaga
     4561 agcggaacgc caagcagcag agttgtcggc aaaacaaaaa gccgaaacag atcgagaagc
     4621 ggcagaatcg gcgaaacgaa aagctgagga agaagagcac cgtcaagcag cccaatccca
     4681 gccgcaacgc cgaaaacgcc gtgccgcacc tcaggattat atggcagctt cccaaaatcg
     4741 tccgaaacgt cgcggacgca gatctactct gccggcaccg ccctcgccat catttgattc
     4801 gtcagcttac gcagcaccca gggccttgca taatccggac tggtatgaaa atgactatga
     4861 agaaatcccc ttggatgcgt tggaagatga aaatgtatct gaatcagttg acacatcaga
     4921 caaacagcct caagacaata cggaacttca tgaaaaatat gaaaatgatt atgaagaaat
     4981 ccccttggat gcgttggaag atgaagatgt atccgaatcg gttgacacat cagacaaaca
     5041 gcctcaagac aatacggaac ttcatgaaaa agttgagaca gtgagtttgc aaccaagagc
     5101 cgctcagccg cgagcccaag ccgccgcgca agccgatgca gtcagcacca atacaaactc
     5161 ggctttatct gacgcaatgg caagcacgca atctatcttg ttggatacag gtgcttcatt
     5221 aacacggcac attgcacaaa aatcacgcgc tgatgccgaa aaaaacagcg tttggatgtc
     5281 gaacatcggt tatggccgtg attatgcttc cgcacaatat cgccggttta gttcgaaacg
     5341 cacgcaaaca caaatcggca ttgaccgcag cttgtccgaa aatatgcaga taggcggcgt
     5401 attgacttac tctgacagtc agcatacttt tgatcaggcg agcggcaaaa atacttttgt
     5461 gcaagccaac ctttatggta agtattattt aaatgatgct tggtatgtgg ccggcgatat
     5521 tggtgcaggc agcttgagaa gccggttaca aacgcagcaa aaagcaaact ttaaccgaac
     5581 aagcatccaa accggcctta ctttgggcaa tacactgaaa atcaatcaat tcgagattgt
     5641 ccccagtgcg gggatccgtt acagccgcct gtcatctgca gattacaagt tgggtaacga
     5701 cagtgttaaa gtaagttcta tgtcagtgaa aacactgacg gccggactgg attttgctta
     5761 tcggtttaaa gtcggcaacc ttaccgtaaa acccttgtta tctgcagctt actttgccaa
     5821 ttatggcaaa ggcggcgtga atgtgggcgg taattccttc gtctataaag cagataatca
     5881 acagcaatat tcagcaggtg ccgcgttact gtaccgtaat gttacattaa acgtaaatgg
     5941 cagtattaca aaaggcaaac aattggaaaa acaaaaatcc ggacaaatta aaatacagat
     6001 tcgtttctaa aatactaaat tcatagcaaa ataaaatgcc gtctgaactc aggcttcaga
     6061 cggcattttt atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
     6121 agatagacgg caaggcgagg caacgccgta ctggtttttg ttaatccact atatcagacg
     6181 gacgaggtta cagtttaatc cctttgagct tcgcgacggt attgatgtct ttgtcgccac
     6241 gacctgagag gttgaccaaa atcacttggt ctttacccat tttcggcgca tttttcaccg
     6301 cccaagcaac ggcgtggctg gattcgagcg cggggatgat gccctcgaag cggcagagca
     6361 agtcaaaggc ttcgagtgct tcgtcgtctt tggcaacggt gtattcgacg cgcttgatgt
     6421 cgtgcagatg gctgtgttcc gggccgatgc cggggtaatc caagcctgcg gaaacagagt
     6481 gtgtacccaa gacttgaccg ttttcgtcct gcatcagata gctgcggaaa ccgtgcaata
     6541 cgccaatcgg tgcgcccgaa gtaatcggcg cggcgtgatc gggggtgttc acgcccaaac
     6601 cgccagcctc cacgccgacg aggcgcacgt tttcttcgcc gatatacggg tggaacaaac
     6661 caatggcgtt cgatccgccg cccacgcagg caacggcaac gtcgggctgt ctgccgatgg
     6721 cttccaacat ttgttctttg gcttcattgc cgatcacgca ttgaaagtcg cgcaccattt
     6781 cgggatacgg cgcgggaccg gcggcggtac cgatgatgta gaatgtgtca tccacgcggg
     6841 cgacccattc gcgcatggct tcgttcatcg cgtctttcaa agtacggctg ccgctgtcaa
     6901 cgccgacgac attcgcgccc aataatttca tacggaacac gttgggcatt tggcgttgga
     6961 tgtcgtccgc acccatgtac acgtcgcaag tcatgccgaa gcgggcggca acagtggcac
     7021 tcgccacgcc gtgctgaccc gcgccggttt cggcgatgac gcgtttttta cccatacgtt
     7081 ttgccaacag tgcctgaccg atggtgttgt tgactttgtg cgcgccggtg tggttcaagt
     7141 cttcgcgctt caaccagatt tgcgcaccgc ccaaatgctc ggacaaccgc gcggcgtggt
     7201 aaacggggct gggcctgccg acataatgtt tcaaatcgcg gtggaattct tcccaaaacg
     7261 acgggtcgtt tttcgctgcc ttataggcat ccgccagctc ttgcaaagcg ggaatcaggg
     7321 tctcggacac gaacagcccg ccgtgttcgc cgaaaaagcc cttctcgtcg ggcgcgtggt
     7381 agttcttcat cggattcttc cttgtgcttc aggttttcaa acggcatttc cgttttttca
     7441 aacatgccgt ccgaacggga aattgtcggc aattataact gcttttcaca caagcccgcc
     7501 atttcaaaaa acgcccgata cgccgccgct tttttcgcca aactcaaagg ataggcacgc
     7561 gaagtcgaag gcaggcgcgt cagcgtcaaa tcccgtccgg caaacggaaa cggcacggtt
     7621 tcgcccgttt tcggcatttt gatgccgcca ccctgaatat cgagcaggat ttccgtcgcc
     7681 ttgccgcctg tcgtacaaat atggcggcac tcgggtattt ttgccaacac cgccgccaaa
     7741 tcgacggttt caactacctt taaaaacttg tcggacgcat tgccgtgttc ccttgccgcc
     7801 ttcaacacgg tcggacagga cgcaattccc cgttcgcgca aaaacgcctt gattttctcg
     7861 gcatcaaacg ctttttcaga caacctttgg aaatgcgccg catcattaaa aaacaccagc
     7921 ccgtaaacgc gccacatatc gttttggaaa ttcggataat gaaactgcat cgcgcgtttg
     7981 tcttccttgg gcggaaacgt ccccatcatc atgaccgcag ccttcggcgg caacacggaa
     8041 tcgaagggat gggtttcgat ttcaagcaga ttttcagaca cgttcaatcc tccaacaggc
     8101 aaaacggcag acagccggtt tttgcaccaa accgccaaac acggcaagct ttcaaacagc
     8161 attatcacgc cctgccgttt tcggttacaa tgccgacatc aaacggatac tgtaaacaca
     8221 tttatgtttc aacacacagg acgacactta aagcaccgcc ctatgtgcca tcctgatttg
     8281 gaaggggtta cgcccctccc aaatagagtc tgattctacc gccccgaagg acagatgtcc
     8341 aagtggcggg gtttcaaccg aaaaggaaac acgatgaaag aacacaaagc ccgcaagcgt
     8401 ttcgggcaga attttttgca ggacacgcgg atcatcagcg atattgtcaa cgccgtgcgc
     8461 ccgcaggcgg atgatgtcgt gattgaaatc ggcccgggtt tggcggcgat taccgaacct
     8521 ttggcgaaaa agctgaaccg cctgcacgtt gtcgaaatcg accgcgacat cgtatgccgt
     8581 ctgaaaacgc tgccgtttgc ggataaactg gtgattcacg aaggcgatgt attgcagttt
     8641 gatttcaacg gcatcgcggg caaaaagaaa atcgtcggca acctgccgta caacatttcc
     8701 acgccgcttt tgttcaagct ggcggaggtg gcggacgatg tcgtcgatat gcactttatg
     8761 ctgcaaaaag aagtggtcga tcgtatggtt gccgcgccga aaagcaacga ctacgggcgt
     8821 ttgggcgtga tgctgcaata ttttttcgat atggagctgc tgattgacgt accgcccgaa
     8881 tcgttcgacc ctgcgccgaa agtggattcc gccgtcgtcc gtatgattcc ggtgaaacac
     8941 cgcatcggca aggcggacga tttcgagcat ttcgccaaac tcgtgaaact cgccttccac
     9001 caacgccgca aaaccatacg caacaacctg aaagaactgg caacagacga agatttgcag
     9061 gcagccggca tcaatccgca agaccgtgcc gaacatatcg cgccggagaa atacgtagcg
     9121 ttgagcaatt atctagcggg caaggccgtc tgaaaaggga gtaagccgat gaagaaatac
     9181 ctgcttttac ccctgatact gccgcttgca gcctgcctga ccaatccgaa cgaccgtttt
     9241 tttgacggca gacgctacac gcttcatcat atggagctcc cgtaacgaca acctgcaacg
     9301 gcaaatgccg tctgaaagaa aagacatgat caaattcaaa aacgtacaca aacatttcaa
     9361 agacctgcac gtcatcaacg gcgtaaactt ggaaatcaaa aaaggcgaag tggtcgtcgt
     9421 ctgcgggcct tcgggcagcg gcaaatccac actcatccgc actgtcaacc agcttgaaag
     9481 catcgaaagc ggcgagattt gggtggacgg tgtcaacgtt gccgacccca aaaccgattt
     9541 gaacaaaatc cgcgaagaag tcggcttcgt attccaaggc ttcaacctct acccgcacct
     9601 gaccgtgttg gaaaacatta ccctcgcacc gatgaaggtt aaggggcaaa atgcggaaca
     9661 ggcggagaaa aaggcaatgg agcttttgga acgcgtcgga ctggcacaca aaaaagacgc
     9721 cttcccttcc caactttccg gcggccagca gcaacgcgtg gcgattgcac gcggtttggc
     9781 gatggaaccg cgcgtaatgt tgtttgacga accgacctcc gcactcgacc ccgaaatggt
     9841 cggcgaagtg ttgaaagtga tgaaagactt ggcagaaagc ggcatgacga tgatgtgcgt
     9901 aacccacgaa atggggtttg cccgcgaagt tgccgaccgc gtgattttcg tcgataaagg
     9961 gcaaatcctc gaagacgaaa cgccggaagc gttttttacc aacccgaaac acgaacgcgc
    10021 caagcagttc ctgcaacagg tcatgaccca ttgatgctat gccgtctgaa aaccgaacgt
    10081 tcagacggca tatcccgaca ccgcaagatg attgagaaag aaaaccatgt attttgttga
    10141 ccgcaccgcc gtcgtcctca agccgaccgc gcgctttttg gaatggctca aaagcaccga
    10201 cgaaaatatg cccgacctga ccatagagca gcttcgtgcc aactgttccg tcttcctcgt
    10261 tccgcagttc gacgaaccgg aagccgtcgt ttcctatttc gacgaacgct accgccagat
    10321 tttcgaggcg gagctggcag gttgggacat cgataaggat aaatggccgc aagatatggg
    10381 gctgaaggcg ttttgggagt ttttcgatac cgaaatccac gatatggtgt tggacatgga
    10441 agaagcggaa ttgaacatta cccccgtgtt cgacaacatg atgtaaggct gtgcgcgcct
    10501 ttcagacggc attgaggccg tcccgcatcc tgaacatcct gaccgtatcg ctccactcgg
    10561 cttcccttgc cgtctgcctg acctggtttt acggccggat gatgtggttc ggtttggcgg
    10621 ctttggcggc aagttacgca tactcgctca ggataacgaa tctgaaacac cgccatgcca
    10681 taaccgccat taccatagac cgcgacggac aggcggaaat cgtatccggc aaagaaaaaa
    10741 cggcaaaggc ggcggcactg tcgggcagca gtatggttac gccttatgcc ttattcttac
    10801 aatgggacac cggcggcaaa accgtccgcc actgcatcac ccacgatatg gcggacccgg
    10861 aatcctaccg gcggctgaaa gtctgggtgc tttggcggca gccgaaaaaa accgccgaaa
    10921 ccgatacacc aagctgattt cagacggcat ttgaacacgg aacactcaat gaaaacactg
    10981 caagactggc tctcccattt ggaaaccgcc cacagcggtg gcttgatcga tatgggcttg
    11041 gaacgcgtag gcgaagtgaa aaaacggatg aagctcacgc cgcaatgccc cgtcgtcgtt
    11101 gtcgcgggaa caaacggcaa aggttcggtc tgcgcctacc tgacacagat ttacaaacag
    11161 gcaggcttta aagtcggtac gctgaccagc ccgcatttat tgcgttacaa cgaacgcatc
    11221 gccgtcaacg ccgaacccgt ttcggacgat acgattattg cctctttcga gcgcatcgaa
    11281 gcggcgcgcg gcgaaatatc gctgacttat tttgaattca atgccttggc ggcggtcgac
    11341 atcttcatgc gcgaacaggt tgacgtgatg atattggaag tcggcttggg cggacgcttg
    11401 gacgcggtca acgcgtttga cggcgattgc gcggtggtta ccagcgtgga tttggaccat
    11461 caggcatttt tgggcgatac ggtcgagcag gtcggctttg aaaaagcagg cgtgttccgc
    11521 agcggcaaac ccgcaatctg cggtcaaaac cccgcgcccg catcattggt cgcacacgcc
    11581 gaagccatag gcgcgaaact gctgatggtg cagcgcgatt tcgagtttca cgcgatggaa
    11641 aacatccaat ggaactaccg cttccggccg caacattcag acggccccgc acgcaaccgc
    11701 aatgccctgc ctttccctgc attacgcggc acataccagc tttccaacgc cgcctgcgcg
    11761 ttgaccgtat tggaatgctt gaacgacaaa ttgcctgtgg acatcggcgc catcaaacgc
    11821 ggtttgctgc tggttgaaaa ccccggccgc ttccaagtcc tgcccggccg tccgctgacc
    11881 gttttggacg tcggacacaa tccccacgct gcccgcgccc tgcgccgcag cctgattaat
    11941 ttggcgtttg cgcaaaaacg caccgccgta ttcagtatgc tgtccgacaa agacatagac
    12001 ggcgtgttgg aaatcgttaa agaccagttt gacgagtggt atatcgcacc gttggacgta
    12061 ccgcgcggca tgacgctcga gagcctgcaa aacaaactgc acgaacacag catcgaaaac
    12121 atacagacat tcgccgccgt ccgcgaagca taccgggcgg cagcctcaaa agcgggcgag
    12181 aacgacagaa tcgtcgtttt cggctcattt cataccgttg ccgatgtcat gtcggtactt
    12241 taaaaggaaa cttctatgtc cgaaaacaaa caaaacgaag tcctgagcgg ttacgaacaa
    12301 ctcaaacggc gcaaccgccg ccgcctcgta acggcaagtt gcctggttgc cgcctcctgc
    12361 atcctgctgg cagccgccct cagttccggc cctgccgaac agactgccgg cgaaacaagc
    12421 ggcgtagaaa acaaagcggc aggtgcggca caaacccctg ccttgaaatc cgccgccgac
    12481 aaaccgcagg acttggcagg cgaagacaag ccttctgccg ccgacagcga aatcagcgag
    12541 cctgaaaacg taggcgcgcc gctggtgctg attaacgacc gcctcgaaga cagcaacatc
    12601 aaaggtttgg aagcatccga gaaactgcaa caggcagaaa ccgccaaaac cgcaccgaag
    12661 caggcaaaac aacgcgctgc cgaaaaagtg ccggcaactg ccgacagtac ggatacggta
    12721 gcggttgaaa aaccgaaacg cactgccgaa acaaaaccgc aaaaagcgga acgcactgcc
    12781 aaagccaagc ccaaagccaa agaaaccaaa accgccgaaa aagttgccga caaaccgaaa
    12841 actgccgccg aaaaaaccaa accggatacg gcaaaatccg acagcgcggt aaaagaagcg
    12901 aaaaaagccg acaaggctga aagcaaaaaa acagccgaaa aagaccgttc ggacggcaaa
    12961 aaacacgaaa cggcacaaaa aaccgacaaa gcggacaaga ccaaaaccgc cgagaaggaa
    13021 aaatccggta aaaaagccgc cattcaggca ggttatgccg aaaaagaacg cgccttaagc
    13081 ctccagcgca aaatgaaggc ggcgggtatc gattcgacca tcaccgaaat tatgaccgac
    13141 aacggcaaag tttaccgcgt caaatcaagc aactataaaa acgcaaggga tgccgaacgc
    13201 gatttgaaca aattgcgcgt acacggtatc gccggtcagg taacgaatga ataacctccc
    13261 cgttgccgac ctcctcgtct tcgccatcat cgccgcctgc atcgtgctat ccgcgatgcg
    13321 cggcgtgatt gcggaggcag gttcgatggc ggcatgggtg gtttccttct tttttgccaa
    13381 actctttgcc gcacccttcg ccgacctcgc ctttgcctcg ttccagccgc gcctgttcgc
    13441 actggcattg tcgtttattt cattattcat cctcgcctat ctgatacaga aaatactccg
    13501 ctcgctgctg acacaagcag tttcctccgt cggcttgggc tttgcaaacc gcattttggg
    13561 cggcgtattc ggtgcattga aaggcgtttt gattgttacc ctgctgatca tgcttgcttc
    13621 aaaaaccgac ctgcccgata ccgaagaatg gcggcaatct tacacactgc cgtttttcgt
    13681 atcgctttcc gaagcagtgt taaaccatgc ggacgacagt gatggagctt tggaagacga
    13741 ttgattgcca tacggatatt tcagacggca tcctctttta tgccgtctga aatgtttttt
    13801 cctttcctca gccatatcac agattatagt ggattaaatt taaaccagta cggcgttgcc
    13861 tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
    13921 atccactata aatcttattc tccataaacc cacctatctg tccttcactc aaatattgtc
    13981 aacaattcag gcaaaaaacc gccgtttgct ttataattcc gttttaccct taaacctcga
    14041 atcggagaag acgatatgtg cggcgtatta ggtttggtca gtcatgcacc cgtgaaccag
    14101 cttctgtacg acggcttgca gatgttgcag cacagggggc aggatgcggc gggcattgca
    14161 acggcggaag gcggcacgtt ccatatgcac aaaggcaaag gcatggtgcg cgaagtgttc
    14221 cgtacacgca atatgcgcga tttgaccggc aacgccggca tcgcccacgt ccgttatccc
    14281 acggcgggca acgccggcag cagcgcggag gcgcagcctt tctacgtcag ctcgccgttc
    14341 ggcatcgttt tggcacacaa cggcaacctc accaacacgg ccgaactgta tgaaaacgtg
    14401 tgcaacaaac acctgcgcca catcaacacc ggctccgatt ccgaagtctt gctcaacgta
    14461 ttcgcgcacg aattgcgccg cgaagtttct aaaaacgccg acccgcaccg gctcaatgcc
    14521 gacaatattt tcaacgccgt tgccgaagtc caccgcctgg tgcgcggcgc atacggcgtg
    14581 gttgccatga ttgcgggcta cggcatggtc gccttccgcg atccttatgg catccgtccg
    14641 ctggcattgg gttcgcaaac cgacgaatca ggcagaaaat cctatgccgt cgcctccgaa
    14701 tccgttgcct tcaatgcgct tgcctacgat ttggaacgcg atatccggcc gggcgaagcg
    14761 gtatttgtcg gctttgacgg cacaatgatt gcccgccaat gcagcgataa ggccaaactc
    14821 agcccctgcc tttttgaata tgtttatttt gcccgccccg actctgtgat tgacggcgta
    14881 tccgtttacc aatcgcgctt ggatatgggc gtgtccttgg cggaaaaaat caaacgcgag
    14941 ctgcccgtgg acgacatcga cgtcgtgatg cccattcccg acaccagccg ccccagcgcg
    15001 atggagcttg ccgtctacct caaaaagcct tatcgcgaag gcttgattaa aaaccgctat
    15061 atcggccgca cctttattat gcccggtcag gcgacgcgca aaaaatccgt gcgccaaaaa
    15121 ctcagcccga tggaaaccga atttgcaggc aaaagcgtgt tgctggtgga cgactccatc
    15181 gtgcgcggga cgaccagccg cgaaatcgtc gaaatggtac gcgcggctgg cgcgcgcaaa
    15241 gtctatatcg cctccgccgc gcccgaagtg cgctatccca acgtatacgg catcgatatg
    15301 cccacacgcg aagagttgat tgccaatggg cgcagcgcgg cggaaattgc cgccgaaatc
    15361 ggcgcggacg gcatcgtgtt tcaagacttg ggcgatttgg aagccgtcgt caaagcactc
    15421 aatccgaaaa tcgaatcttt cgattcgtcc tgtttcaacg gcatttatca aaccggcgac
    15481 atcgatgatg cctacctcga ccgcctgtct gccgagaaat ccggctgcgg cggcctgaaa
    15541 atccacccga gcaggatgga acacagcatc agcatcagcg atgcgggcga cgaagaataa
    15601 ggcttcccaa accgcgatgc cgtctgaaat ccgaacgccg ggttttcaga cggcatttcc
    15661 catatcggca tcacatttcc catttcatcc gcacgcaata tgagtaccga aaccaaaaac
    15721 tacattacgc ccgccggatg gcaggcattg aaagacgagc tttaccggct ggtcaacaaa
    15781 gaacgccccg aaatcgtcca aatcgtcaac tgggcggcag gcaacggcga ccgcagcgaa
    15841 aacggcgact atctttacgg caaacgccgt atgcgcgaaa tcgaccgccg catccgtttc
    15901 ctgaccaaac gtttggagtc tgccgtcgtc gtcgatcccg aattgcgcga agcgaccgac
    15961 caagtgtttt tcggcgcgac ggtcgggctg ttgcgcggcg acgggcgcga gcaaaccgtc
    16021 aaaattgtcg gcatcgatga aatcgatacc gcgcaaaaca aaatttcttg gatttcgccg
    16081 ttggcgcgtt gcctgattaa agcgcgcgaa ggcgacgaag tggttttgaa cacgccggaa
    16141 gggcgtgagg aaattgaaat cctttctgtc gaatacatca aaatcgactg atcgggaatg
    16201 ccgtctgaaa cggcacaaac gcatcgtttc ccaaataaag gaataccgaa tgaggaaaat
    16261 ccgcaccaaa atctgcggca tcaccacact ggaagacgca gcggccgccg cagcggcagg
    16321 tgcggatgcc gtcgggctgg tctttttcca aggcagcagc cgggccgtcg atattgcccg
    16381 cgcaaaaaaa atcaccgccg cactgccgcc gtttgtcagc gttgtcgccc ttttcgtcaa
    16441 cgaaagcgcg caaaacatcc gccgcatcct tgccgaagtg ccgatacaca tcatccaatt
    16501 ccacggcgac gaagacgacg cattctgccg ccagttcgac cgcccctata tcaaggccat
    16561 tcgtgttcag acgacatcag acatccgaaa cgccgccgac cgcttccccg acgctcaggc
    16621 actgcttttc gatgcctacc atccttcgga atacggcggc accggacacc gcttcgactg
    16681 gacgctgctg gcggaatatt cgggcaaacc gtgggtactt gccggcgggc tgacccctga
    16741 aaacgtcggc gaagccgtcc gcatcaccgg agcggaagcg gtcgatgtat ccggcggcgt
    16801 ggaagcgtct aaaggcaaaa aagacccagc caaagtcgcc gcctttatcg caaccgccaa
    16861 ccgcctatcc cgttaaagca acaaaaattg ccgccggaat gacttatagt ggattaacaa
    16921 aaaccagtac ggcgttg
//
LOCUS       DAWFJW010000037        15954 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFJW010000037 DAWFJW010000000
VERSION     DAWFJW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.037"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            241..1305
                     /locus_tag="WGR19_000969"
     CDS             241..1305
                     /locus_tag="WGR19_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0798519.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1446..1769
                     /locus_tag="WGR19_000970"
     CDS             1446..1769
                     /locus_tag="WGR19_000970"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0798520.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1841..2164
                     /locus_tag="WGR19_000971"
     CDS             1841..2164
                     /locus_tag="WGR19_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0798521.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2211..2414
                     /gene="tatA"
                     /locus_tag="WGR19_000972"
     CDS             2211..2414
                     /gene="tatA"
                     /locus_tag="WGR19_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0798522.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2418..3104
                     /gene="tatB"
                     /locus_tag="WGR19_000973"
     CDS             2418..3104
                     /gene="tatB"
                     /locus_tag="WGR19_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0798523.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASTETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKHR
                     AKLKLRVRKQ"
     gene            3118..3888
                     /gene="tatC"
                     /locus_tag="WGR19_000974"
     CDS             3118..3888
                     /gene="tatC"
                     /locus_tag="WGR19_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0798524.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVIGILVCFFGMMPFAQQ
                     LYTFIANPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3941..4549
                     /gene="maf"
                     /locus_tag="WGR19_000975"
     CDS             3941..4549
                     /gene="maf"
                     /locus_tag="WGR19_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0798525.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4558..4911
                     /locus_tag="WGR19_000976"
     CDS             4558..4911
                     /locus_tag="WGR19_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0798526.1"
                     /translation="MNKQTAYFLASFSLIALIILSLAWELWIAPLRPGGSWLALKTLP
                     LCLPLSGILKKKIYTYQYSSMLILIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4901..6715
                     /locus_tag="WGR19_000977"
     CDS             4901..6715
                     /locus_tag="WGR19_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0798527.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPAPDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6905..7582
                     /locus_tag="WGR19_000978"
     CDS             6905..7582
                     /locus_tag="WGR19_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0798528.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7595..9001
                     /locus_tag="WGR19_000979"
     CDS             7595..9001
                     /locus_tag="WGR19_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0798529.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9107..11026
                     /locus_tag="WGR19_000980"
                     /pseudo
     CDS             9107..11026
                     /locus_tag="WGR19_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
     gene            11071..11316
                     /gene="rpsP"
                     /locus_tag="WGR19_000981"
     CDS             11071..11316
                     /gene="rpsP"
                     /locus_tag="WGR19_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0798530.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11332..11841
                     /gene="rimM"
                     /locus_tag="WGR19_000982"
     CDS             11332..11841
                     /gene="rimM"
                     /locus_tag="WGR19_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0798531.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            11841..12590
                     /gene="trmD"
                     /locus_tag="WGR19_000983"
     CDS             11841..12590
                     /gene="trmD"
                     /locus_tag="WGR19_000983"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0798532.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            12607..12972
                     /gene="rplS"
                     /locus_tag="WGR19_000984"
     CDS             12607..12972
                     /gene="rplS"
                     /locus_tag="WGR19_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0798533.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            12992..13297
                     /locus_tag="WGR19_000985"
     CDS             12992..13297
                     /locus_tag="WGR19_000985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798534.1"
                     /translation="MPSEPSDGISYCRNIRNGTAPKSTPLPAHPESPSEPNKIYRPNV
                     IQYHISHITINALPPSLSKPQIIMTPSLLLSGLTFRLILALIAVSLLWGVYFWAVSA"
     gene            13294..14019
                     /locus_tag="WGR19_000986"
     CDS             13294..14019
                     /locus_tag="WGR19_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0798535.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14043..14918
                     /locus_tag="WGR19_000987"
     CDS             14043..14918
                     /locus_tag="WGR19_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0798536.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            14995..15909
                     /locus_tag="WGR19_000988"
     CDS             14995..15909
                     /locus_tag="WGR19_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0798537.1"
                     /translation="MKHPKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLILLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSTYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4154 a         5087 c         3518 g         3195 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccactataaa tgccatctga agccgattcg gttttcagac
       61 ggcatcatta tttgacctga cgcaaacaga aagccttccg cgtgcttaaa cacatctctt
      121 gtagtaaaat atctaaacag attcaactgg aaacggtcag gcttcttcac gccattccca
      181 tttcaaccgc cgcctgccag attcagacgg tattcctact tgaaaacaag gagcgttaca
      241 atgaaagcag cacgttttta cgacaaaggc gacatccgca tcgaagacat ccccgaaccg
      301 accgtcgccc ccggcaccgt cggcatcaat gttgcctggt gcggcatctg cggtaccgac
      361 ctgcacgaat tcatggaagg cccgattttc attccgcctt gcggtcatcc gcacccgatt
      421 tccggcgagt ccgcacccgt cacgatggga cacgagttct ccggcgtggt ctatgccgtc
      481 ggcgaaggcg tggacgacat caaagtcggt caacacgtcg ttgtcgagcc ttacatcgta
      541 cacgacgacg taccgaccgg cccgggcagc aactaccacc tctctaaaga catgaacttc
      601 atcggcttgg gcggctgcgg aggtggcttg tctgaaaaaa tcgctgtcaa acgccgttgg
      661 gtgcatccga tttccgacaa aatcccgttg gatcaagccg ctttgatcga accgctgtct
      721 gtcggacacc acgcctatgt acgcagcggc gcgaaagcag gcgatgtcgc attggtcggc
      781 ggtgcaggcc cgatcggttt gctgttggct gccgtgttga aagccaaagg catcaaagtc
      841 atcatcaccg agttgagtaa agcacgcaaa gacaaagcgc gcgaatccgg cgttgccgac
      901 tacatcctcg acccgtccga agtcgatgtt gtcgaagagg tgaaaaaact gaccaacggc
      961 gaaggcgtgg acgtggcatt cgaatgtacc agcgtgaaca aagtgttgga tactttggtc
     1021 gaagcctgca aacctgccgc caatttggtt atcgtatcca tctggagcca ccctgcaacc
     1081 gtcaacgtcc acagcgtcgt gatgaaagag ctggatgtgc gcggcacgat tgcctactgt
     1141 aacgaccacg ccgaaaccat caaactggtc gaagaaggca aaatcaacct cgaacccttc
     1201 atcacccagc gcatcaagct ggacgaactg gtttccgaag gcttcgagcg tctgatccac
     1261 aacaacgaat ccgccgttaa aattattgtg agtccaaacc tgtaagcaaa ttacatttgc
     1321 aacagcacaa atgccgtctg aaacgctcag acggcatttt ccaacgaagc gcaacaggcg
     1381 tattgcccga gagcagccgt ttagcctatc attccgaaca aacagaaaca aacagaagga
     1441 aaatcatggg agattccgta ctatccgcca tccaacaaac catcacccag cgaaaatctg
     1501 ccaatccgtc cgaatcttac gtcgcacagc tcttgcataa gggcgaagac aaaatcctaa
     1561 aaaaagtgat tgaagaggcg ggcgaagtgt tgatggcatc caaagacaaa aacccgtccc
     1621 acctggttta cgaagttgcc gacttatggt ttcacaccat gattcttctg acacaccacg
     1681 acctgaaggc ggaagacgta ttggacgaac ttgcgcgccg ccaaggtttg tcgggcttgg
     1741 ccgaaaaagc cgctcgcaca gaatcttgaa tttatattaa aatccgcact ttcccacatt
     1801 caatccgtct gaccgctgtt cagacggcaa cggagccgtt atggacaact gtattttctg
     1861 caaaatcgcc gccaaagaca ttccggcgca aaccgtctat gaagacggcg aaatggtttg
     1921 tttcaaagac atcaaccccg ctgctccggt tcatctgctg ctgattccca aagtccattt
     1981 cgattcgttg gcacacgccg cgcccgaaca tcagcccctt ttgggaaaaa tgatgctgaa
     2041 agttcccgaa atcgccaaag cggcaggact ggcagacggc ttcaaaaccc tgattaacac
     2101 cggaaaaggc ggcggacaag aggtcttcca cctgcatata cacatcatgg gcacacccgt
     2161 ataaaccgtt atttcacaat caacccctaa tacttactta aggatacatc atgggcagtt
     2221 tttctctgac gcactggatt atcgtactga ttatcgtcgt tttgatattc ggcaccaaaa
     2281 aactgcgcaa cgtcggcaaa gacctcggcg gtgcggttca tgacttcaaa caggggctga
     2341 acgaaggtac agacggcaaa gaagcccaaa aagacgatgt aatcgaacac aaaaaagacg
     2401 aagacaaagc gtaatttatg tttgatttcg gtttgggcga gctggttttt gtcggcatta
     2461 tcgccctgat tgtcctcggc cccgaacgcc tgcccgaggc cgcccgcacc gccggacggc
     2521 tcatcggcag gctgcaacgc tttgtcggca gcgtcaaaca ggaatttgac actcaaatcg
     2581 aactggaaga actgaggaag gcaaagcagg aatttgaagc tgccgccgct caggttcgag
     2641 acagcctcaa agaaaccggt acggatatgg aaggcaatct gcacgacatt tccgacggtc
     2701 tgaagccttg ggaaaaactg cccgaacagc ggacacctgc cgatttcggt gtcgatgaaa
     2761 acggcaatcc gcttcccgat gcggcaaaca ccctatcaga cggcatttcc gacgttatgc
     2821 cgtccgaacg ttcctacgct tccaccgaaa cccttgggga cagcgggcaa accggcagta
     2881 cagccgaacc cgcggaaacc gaccaagacc gcgcatggcg ggaatacctg actgcttctg
     2941 ccgccgcacc cgtcgtacag accgtcgaag tcagctatat cgataccgct gttgaaaccc
     3001 ctgttccgca caccacttcc ctgcgcaaac aggcaataag ccgcaaacgc gatttgcgtc
     3061 cgaaacaccg cgccaaactt aaattgcgcg tccgtaaaca ataaagaggg caatccggtg
     3121 tccgaaacac aaaacgaaca aaccgtccaa ccgcttgtcg agcatctcat cgagctgcgc
     3181 cgccgcctga tgtggatggt tatcggcatt ctcgtctgct ttttcggcat gatgccgttt
     3241 gcccaacaac tctatacttt tatcgccaac ccgctgatgg caaacctgcc caaagacacc
     3301 agcatgattg ccaccgatgt catcgcaccg tttttcgtgc cggtcaaagt taccctgatg
     3361 gcggcatttt taatttcgct gccgcatacg ctctaccaaa tctgggcatt tgtcgcgccc
     3421 gcactctacc aaaacgaaaa acgcctgatt acgccgctcg tcctctccag cgtcagcctg
     3481 tttttcatcg gcatggcatt tgcctacttt ctggttttcc ccgtcatttt caaattcctt
     3541 gccagcgtta cccctgtcgg tgtcaatatg gcgacagaca tcgacaaata cctgtctttt
     3601 atcttaggca tgtttgttgc gttcggcaca acctttgaag tccccattgt cgttatcctg
     3661 ttaaccaaaa ttggtgtggt aacaaccgaa cagctcaaac gcgcccgccc ctatgtgatt
     3721 gtcggcgcgt ttgtcattgc cgccatcatc acgccgcccg atgtgatttc acaaaccctg
     3781 cttgccattc cgctgattct cttatacgaa gcaggtattt ggttcggacg ctttttcacg
     3841 ccacgttcag aacaggatgg cgacatacag ccgcctgcaa caacctgaca ctatgccgtc
     3901 cgaacctccg cctcataccg caacagatta aggaatacct ttgaataccc tctatttagg
     3961 ttcaaacagc ccgcgccgaa tggaaatcct gacgcagttg ggctatcagg tcgtcaagct
     4021 gcctgccgac atcaatgaaa cggtcagaca gaacgaagac cctgcccgtt acgttcaaag
     4081 gatggcagaa gaaaaaaacc gaaccgccct gaccctcttt tgcgaaacca acggcacaat
     4141 gcccgatttc cccctgatta ccgccgacac ctgcgtcgtt tcagacggca tcatattggg
     4201 caaaccccgc tcccaagccg aagcaatcga atttttaaac cgattgtccg gcaaacaaca
     4261 taccgtcctg actgccgtct gcattcatta tcgcggcaat gcggaaaacc gcgtccaaac
     4321 caaccgcgtt gtttttaagc ccctgagttc ggaagaaatt tccgcctatg tgcaaagcgg
     4381 cgaaccgatg gacaaagccg gtgcctacgc cgtacaaggc ataggtggca tctttatcca
     4441 atctatcgaa ggcagcttca gcggcattat gggactgccc gtttatgaaa ccgtttcgat
     4501 gttgcaggat ttgggatacc gctccccctt gtccgccctt aaaccgtaaa gtccgccgtg
     4561 aacaaacaaa ccgcttactt ccttgcctct ttcagcctga tcgcactgat aatcctgtcc
     4621 cttgcttggg aactgtggat tgcaccgtta cgcccgggcg gttcgtggct tgcgctcaaa
     4681 accctgcccc tctgcctgcc gctttcaggc atcctgaaga aaaaaatcta cacctaccaa
     4741 tacagctcca tgttgattct gatttatttt gccgaagccg tcatgcgcct gttcgacgcc
     4801 tatccggcag aacaaatttg cgccgcgctt tccgcagtat tcagcatcat cttcttcata
     4861 tcatgcctgt cgttcgtcaa acaatacaag gaaacaaaca atgcccgctg aaacgaccgt
     4921 atccggtgcg caccccgccg ccaaactgcc gatttacatc ctgccctgct tcctttggat
     4981 aggcatcgtc ccctttacct tcgcgctcag gcagcaaccg tcgcccgact tttaccacga
     5041 tgctgcagcc gcagccggcc tgattgtcct gttgttcctc acggcaggaa aaaaactgtt
     5101 tgatgtcaaa atccccgcca tcagcttcct tctgtttgca atggcggcgt tttggtatct
     5161 tcaggcacgc ctgatgaacc tgatttaccc cggtatgaac gacatcgtct cttggatttt
     5221 catcttgctc gccgtcagcg cgtgggcctg ccggagcttg gtcgcacact acggacaaga
     5281 acgcatcgtt accctgtttg cctggtcact gcttatcggc tccctgcttc aatcctgtat
     5341 cgtcgtcatc cagtttgccg gctgggaaga cacccccctg cttcaaaaca tcatcgttta
     5401 cagcgggcaa ggcgtaatcg gacacatcgg gcagcgcaac aacctcggac actacctcat
     5461 gtggggcata ctcgccgccg cctacctcaa cggacaacga aaaatccccg ccgccctcgg
     5521 cgcaatctgc ctgattatgc agaccgccgt tttaggtttg gtcaattcgc gcaccatctt
     5581 gacctacata gccgccatcg ccctcatcct tcccttctgg tatttccgtt cggacaaatc
     5641 caacaggcgg acgatgctcg gcatagccgc agccgtattc cttaccgcgc tgttccaatt
     5701 ttccatgaac accattctgg aaacctttac cggcatccgc tacgaaactg ccgtcgaacg
     5761 cgtcgccaac ggcggtttca cagacttgcc gcgccaaatc gaatggaata aagcccttgc
     5821 cgccttccag tccgccccga tattcgggca cggctggaac agttttgccc aacaaacctt
     5881 cctcatcaat gccgaacagc acaacatata cgacaacctc ctcagcaact tgttcaccca
     5941 ttcccacaac atcgtcctcc aactccttgc agagatggga atcagcggca cgcttctggt
     6001 tgccgcaacc ctgctgacgg gcattgccgg gctgctgaaa cgccccctga cccccgcatc
     6061 gcttttcctg atctgcacgc ttgccgtcag tatgtgccac agtatgctcg aatatccttt
     6121 gtggtatgtc tatttcctca tccctttcgg actgatgctc ttcctgtccc ccgcagaggc
     6181 ttcagacggc atcgccttca aaaaagccgc caatctcggc atactgaccg cctccgccgc
     6241 catattcgca ggattgctgc acttggactg gacatacacc cggctggtta acgccttttc
     6301 ccccgcccct gacgacagtg ccaaaaccct caaccggaaa atcaacgagt tgcgctatat
     6361 ttccgcaaac agtccgatgc tgtcctttta tgccgacttc tccctcgtaa acttcgccct
     6421 gccggaatac cccgaaaccc agacttgggc ggaagaagca accctcaaat cactaaaata
     6481 ccgcccccac tccgccacct accgcatcgc cctctacctg atgcggcaag gcaaagttgc
     6541 agaagcaaaa caatggatgc gggcgacaca gtcctattac ccctacctga tgccccgata
     6601 cgccgacgaa atccgcaaac tgcccgtatg ggcaccgctg ctacccgaac tgctcaaaga
     6661 ctgcaaagcc ttcgccgccg cgcccggtca tccggaagca aaaccctgca aatgacccgc
     6721 gccggcggat acggataccg cccgaaatgt aaagctccat gcaagacatt gcaaaaaaca
     6781 gcaaaccggt agggaaatac gctatcaaaa aacattgcag ccgtgttaag ataaaccgtc
     6841 aaacaatctt ttcacgcccc gcccgaaaca gggtcggggc atacccttac gaaaaggaaa
     6901 caccatgagc cgcgtattac tcgtagatga cgatgccctg ctgaccgaac tgctgaccga
     6961 atacctgagc gccgaaggtc tgaacgtccg cagcgttccc gacggggaag caggcgtaca
     7021 ggaaatcctg agcgggcagt acgatgtagt cgtattggat tccatgatgc ccaaaatgaa
     7081 cggcttggat gtcttgaaaa acgtacgcgc ccgaagcacc gtccccatca tcatgctgac
     7141 cgccaaaggc gacgacatcg accgaatcat cggcttggaa atgggcgcgg acgactatgt
     7201 ccccaaaccc tgcacaccac gcgaactctt ggcacgcatc aatgccatcc tgcgccgcgc
     7261 acaacacagc ggcgaacaga acaacgcacc caacagcatc tccgtcagcg atgtcgtcct
     7321 gtaccccgcc aaacgccagg catccgtcaa agacatgccg ctcgaactga ccagcaccga
     7381 attcaacctg ctcgaagtcc tgatgcgcca tgccggacag gtagtcagca aagaaaccct
     7441 gtccgtcgaa gcactcgacc gcaagctggc aaaattcgac cgcagtatcg acgtacacat
     7501 ctccagcatc cgccacaagt tgggcgatgc ctctctgatt caaaccgtac gcggcttggg
     7561 ctacctgttt gtcaaaaact gaagcaacgg gcaaatgaaa ctgttccaac gcattttcgc
     7621 cacattttgc gcagttatcg tctgtgcaat ctttgtggcg agtttttctt tttggctggt
     7681 gcagaacacc cttgccgaaa accagttcaa ccaacgccgc accatcgaaa ccactttgat
     7741 gggcagcatc atttccgcat tccgggcacg cggggacgcg ggcgcgcgcg aaatcctgac
     7801 ggaatggaaa gacagccccg tctcatcggg cgtgtacgtt atacagggcg acgagaaaaa
     7861 agatatcctg aaccggtata tcgacagcta caccatcgaa cgcgcccggc ttttcgccgc
     7921 cggacacccg cattccaacc tcgtccatat cgaatacgac cgcttcggcg aagaatacct
     7981 gttcttcacc aaagactggg acaaactcca agcccgccgc ctgcccagcc ccctgttgat
     8041 ccccggcctg ccgctcgccc cgatttggca cgaactcatc atattgtcct tcatcatcgt
     8101 cgtcggactg ctgatggcat atatcctcgc cggcaatatc gccaaaccca tcagaatctt
     8161 aggcaacggc atggacaggg tggcaaacgg agaacttgaa acccgtatct cccaacaggt
     8221 cgacgaccgc gacgacgaat tgtcccatct tgccatccaa ttcgacaaaa tggtggaaaa
     8281 actcgaaaaa ctcgttgcca aagaacgcca cctgctccac cacgtttccc acgaaatgcg
     8341 ctccccgctt gcccgtatgc aggcgattgt cggactgatt caggcgcagc cccaaaaaca
     8401 ggaacaatat ctcaaacggc tggaaggcga actgacccgc atggatacgc tggccgggga
     8461 actgttaacc ctgtcccgtc tcgaaacttc caatatggct ttggaaaaag aaagcctgaa
     8521 actcctgccc ttcctgggca acctggtaga agacaatcaa agcattgccc agaaaaacgg
     8581 acaaacggtt accctgtctg ccgacggaaa aatccccgaa aacacaacca tccttgccaa
     8641 cgaaagctac ctgtaccgcg ccttcgacaa cgtcatccgc aacgccgtca actacagtcc
     8701 cgaaggcagc accatcctga tcaacatcgg acaagaccac aaacactgga taatcgacgt
     8761 taccgacaac ggccccggcg tggacgaaat gcagctcccg cacatcttca ccgctttcta
     8821 ccgtgcagac tccagtgcca acaaacccgg aacaggactg gggcttgcat tgacccaaca
     8881 tattattgaa cagcactgcg gcaaaatcat cgccgaaaac atcaaaccga acgggctgcg
     8941 gatgcgcttc atcctaccca agaaaaaaac cggttccaaa acagaaaaaa gtgcgaactg
     9001 accataatgc cgtctgaacc ggcttcagac ggcattgcac aaacagttac cccaaaacaa
     9061 acacacagcg ggtgcatctc caaaaatcac gcacataccg tccgcaatcg acaacatcct
     9121 gaacaaactc cgtcaaggca catcccgcca catcagcctg catcccggca tcagccccat
     9181 cacatccgcc gattggctca accgcttcgg acacagcctg cacaccaaaa ccgccgtcct
     9241 acaccacaac cctgaagaga atcagcgcaa actgttcagc gcaatccgcc aatttacccg
     9301 ccatacgccg ctgattctcc acatcacctg ccgcacgaca gaaaccgacc gtgccgtact
     9361 gcactgcctc gcccgacact gcaacttcct catcagtttc aacgccgacg acctcgaagc
     9421 cgcactgcgc gcccaactgt ccgaccttcc acccctgtcc cgactcgaca tcctgtccga
     9481 cacgcctgcc gaatggctga acgcgcacgc gccaaaaacc ctcgccctcc actttcccaa
     9541 ccttccccac cacatccgca acggacacct gaccggcaca cccacaccct caatctgcca
     9601 cgacatcgcc tcacgtcagc ttgcccgccc cgacacccaa gccgtcctga ccatcctcag
     9661 cccctccgga cacgaggatt acaaaaaaac agcacgcgcc cttatccgcc tgtccgaaca
     9721 gaccgccaaa cccctgctcg tcagcagccc cttttcagac ggcataaccc atttcgacac
     9781 ccccactcag gcaatccgca ccctttccta ccgcaacacc gccgccgccc tgaaacaggc
     9841 acagctcgac attgcaccgc cgcagccatg ccgtctaaaa acaccgcaac cccaaaacat
     9901 caaaaaagcc cttgcagcgg caaacccctc cctgctcgcc gaagccctgc acctcccccc
     9961 ctaccggcac actacccata acgccgtcca attccaattc ggcagccacc ccctctatgg
    10021 cgacatcctg accgcacgct gcaacggaca aaccactgcc gtactcccgc cgtttaccac
    10081 gctcgacagc cgccaccttg cccgctttgc cgaactcgac ggcacacaaa ccctcgacca
    10141 gttcctgcac acactgacca tcattcccga ataccgccaa cacattctcg gcatcaccct
    10201 caacctcaac ggcggacaat acagcagcga tttcatctta aagacacccg aaacacacga
    10261 cacgcccaaa cgcaagaaca caggcaaagc cgcccaaacc ctcgaacatg ccgccgcaaa
    10321 aatgcagagt gccgccgcat acctgaaaca caaaaacccg acagccgccg aatttctccg
    10381 ccacacaagc gaagccgccg cagaactgct cggcagcaaa actgaaaccg ataaaaaaac
    10441 acccaacgtg ctcccgccct accctgaggc acacccaaaa atactgtttc taaaaaacaa
    10501 catgaccgtt acagtaagcc cgctgctgcc cgaagatgcc gaggccaaac aaaaattcat
    10561 ccgaaaactc agccccgaag cacggtacac acgcttcatg acccacacca acgaactgcc
    10621 cgcagccacg ttggcacgcc tgtgcaaccc cgattaccac tgtgaagccg catggacggc
    10681 aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac agccgcctga accgcaacga
    10741 atgcgaattc ggcatcacac tggcggaaca tatgcgcggc agcggactgg cacagaaaat
    10801 gatggaactc atcatccaaa ccgccgcaca gcaaggctac cggactatga gtgccgacat
    10861 tctcaaaacc aatactccca tgatcaaact tgctgaaaaa tcaggattta ccctcaagga
    10921 atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac cttgcggcag acaaaacaac
    10981 agaaaaaaca aataaaaact tgcacaccga ccacaaaata acctaaaatc ggcagtttcc
    11041 atatatccga ttttctcaaa aggactcaaa atggtagtta tccgtttggc acgcggcggc
    11101 tcgaaacacc gccccttcta caacgtcatc gttactgact cacgcagccg ccgcgacggc
    11161 cgcttcatcg aacgcgtagg cttctacaac cccgtagcca acgaaaaaca agagcgcgtc
    11221 cgcctcaatg cagaccgcct gaaccactgg attgcacaag gcgcgcaagt cagcgactcc
    11281 gttgcaaaac tgattaaaga acaaaaagcc gcctaatccg catttgccgc catgacagac
    11341 actcaaaacc gggtagccat gggctacatc aaaggcgtat tcggcataaa aggctggttg
    11401 aaaattgccg ccaacaccga atattccgac agccttttgg actaccccga gtggcatttg
    11461 gtcaaggacg gcaaaaccgt cagcgttacc cttgaagccg gaaaagtcgt caacagcgaa
    11521 ctccaagtca aattcgaagg cataaacgac cgcgacttgg cattctcatt gcgcggttac
    11581 accatcgaaa taccccgtga agcattcgcc ccgacagaag aagacgaata ctactgggca
    11641 gacctggtcg gcatgaccgt cgtcaacaaa gaccataccg ttttaggcaa ggtaagcaac
    11701 ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg gagaacacgg gcagattctg
    11761 attccgttcg tttcccaata tatcgaaacc gtcgataccg gcagcaagac cattactgcc
    11821 gactggggtt tggactactg atgcttatcc aggcagttac cattttcccc gaaatgttcg
    11881 acagcattac ccgctacggc gtaacgggac gcgcgaacag acagggaatc tggcagtttg
    11941 aagcagtcaa tccccgaaag tttgccgaca acagattggg ctacatcgac gaccgcccgt
    12001 tcggcggcgg tccgggaatg attatgatgg ctccgccgct tcatgcggca atagaacacg
    12061 ccaaagcaca atcttcccaa gctgcaaaag tcatttacct cagcccccaa ggaaaaccgc
    12121 tgacacaccc aaaagcggca gaactggcag aacttccgca tctgattctg ctgtgcggac
    12181 gctatgaggg aatagacgaa agactgctgc aaaccagcgt cgatgaagaa atcagcatcg
    12241 gagacttcgt tgtttccggc ggagagcttc ccgccatgat gctgatggat gcggtattga
    12301 ggctcgtacc cggcgtattg ggcgatatgc agtctgccga acaagattcg ttctcaagcg
    12361 gcattttgga ctgcccccac tacaccaaac ccttagaatt tcaaggcatg gctgttccgg
    12421 aagtattacg ttccggcaat catggcttga tagcggaatg gcggttggaa caatcgctgc
    12481 gccgcacctt ggagcgcaga cccgatcttt tggaaaagcg cgttttaatc ccaaaggaat
    12541 cccgcctctt agaaaccatc cggcaagagc aacgggaaat ccaatcataa tttaggaaaa
    12601 aaaacaatga acctgattca acagctcgag caagaagaaa ttgcccgcct gaataaagaa
    12661 atccccgaat tcgcaccggg cgacaccgta gtcgtatccg tacgcgtcgt ggaaggtacc
    12721 cgcagccgtc tgcaagccta cgaaggcgtt gtcattgcac gccgcaaccg tggtctgaac
    12781 agcaacttca tcgtccgcaa aatctccagc ggcgaaggcg ttgaacgtac tttccaactg
    12841 tactctccga ccgtcgaaaa aatcgaagtc aaacgccgtg gcgacgtacg ccgtgccaaa
    12901 ctgtactacc tgcgcggtct gaccggcaaa gctgcacgca tcaaagaaaa actgcctgca
    12961 cgcaaaggtt gattcaaacc cgttttcccc aatgccgtct gaaccttcag acggcatttc
    13021 ttattgccgc aacatccgca acggaacagc accaaaaagc acaccccttc cggcacatcc
    13081 cgaatccccg tccgaaccga acaaaattta cagaccaaac gttatacagt atcatatcag
    13141 ccatataacg ataaacgctt taccgccttc cctttcaaaa ccgcaaatca tcatgacacc
    13201 ttccctttta ctatcaggat tgactttccg cctcatcctt gccctgattg ccgtatccct
    13261 tttatggggc gtttactttt gggcagtatc cgcatgagca tcattgtcga aaacctgacc
    13321 gtcagctacc gccgccgacc tgccgtgcac catgtggaca ttacttttga agaacatagt
    13381 atgtgggcgg ttttcggtcc caacggcgca gggaaatcca cctttctcaa atccttaatg
    13441 ggattgcagc ctatcgatac gggcagcatc cggctggacg gattgacccg tcagaacatt
    13501 gcctaccttc cccagcagtc cgacatcgac cgctcgcagc ctatgaccgt tttcgacttg
    13561 gcggcaatgg ggctatggta tgaaatcggt tttttcagag gaataaatgc cgttcaaaaa
    13621 caacgcgttc acgaatcttt ggaacgcgtc gggatgcggc agtttgccgg ccgccaaatc
    13681 gcccacctct caaacggaca attccagcgt gtcctttttg cccgaatgct ggttcaaaac
    13741 gccaaattcc tacttctaga cgaacctttc aacgctgttg atgcacggac gacctacgaa
    13801 cttctcgacg tattgcagaa atgccattgc ggcggacacg ccatcatcgc cgtactgcac
    13861 gattacgaac aagtccgtgc ctactttccc aataccctgc tgctcgcccg cgaaaaaatt
    13921 gcggcaggcg caaccgagac cattctgaca gaaccctacc tcgcccaagc caacgccaaa
    13981 atgcagcaac aggaaagccc cgagtggtgc gcgtcataaa tgccgtctga aaccgaaaaa
    14041 ccatgaacct ctacgacctg ctccttgccc cctttgcaga attcgacttt atgcgctacg
    14101 ccctcgcatc cgtcttctgc ctgtccctca gtgccgcacc cgtcggcgta ttcctcgtca
    14161 tgcgccgtat gagcctgata ggcgacgcat tgagccacgc cgtcctgccc ggtgccgccg
    14221 tcggctacat gtttgccggc ttgagcctgc ccgccatggg tttgggcggc gtagccgcag
    14281 gcatgctgat ggcactgctt gccggacttg ttagccgctt caccaccctg aaagaagatg
    14341 ccaactttgc cgccttttat ctcagcagcc tcgccatagg cgtagtcctc gtcagcaaaa
    14401 acggcagcag cgtcgatctg ctgcatctgc ttttcggctc tgtacttgcc gtcgatattc
    14461 ctgccctgca gctcatcgcc gccgtatcca ccctcacact gcttaccctt gccgtcatct
    14521 accgcccgct cgtactcgaa agcatcgacc ccctgtttct caaatctgtc ggcggcaaag
    14581 gcgggctttg gcacgtcctc tttctcgtcc tggtcgtcat gaacctcgta tccggctttc
    14641 aagccctcgg cacactcatg tccgtcggac tcatgatgct gccagccatt accgcccgcc
    14701 tgtgggcgaa gcacatgggc gcactcatcc tcctatccgt tctgacagcc ctgctgtgcg
    14761 gcttgagcgg gctgctcatt tcctaccaca tcgaaatccc ttccggcccc gccatcatcc
    14821 tctgttgcag cgtcctttat ctcttttccg tcatactcgg caaagaaggc ggcattctga
    14881 ccaaatggct caaaaaccac cgccaccaca ccacctgacc ccgcaagccg cttgaacaaa
    14941 caggcggctt gttttaccat aacagatgtt acgttatatc acaggagaac agttatgaaa
    15001 caccccaaac tcacccttat tgccgcattg ctaaccaccg ccgcaactgc cgcccccctg
    15061 cccgtcgtaa ccagtttcag cattttaggc gacgtagcca aacaaatcgg cggagagcgc
    15121 gtatccatac aaagtttggt cggagccaac caagatacgc acgcctatca catgaccagt
    15181 ggcgacatta aaaaaatccg cagtgcaaaa ctcatcctgc tcaacggctt aggacttgaa
    15241 gccgccgaca tccaacgtgc cgtcaaacag agcaaagtat cctatgccga agcgaccaaa
    15301 ggcatccaac ccctcaaagc cgaagaagaa ggcggacacc atcatgacca cgaccatgac
    15361 cacgaaggac accaccatga ccacggcgaa tttgacccgc acgtctggaa cgaccccgtc
    15421 ctcatgtcca cctatgccca aaacgtcgcc gaagccctga taaaggccga ccccgaaggc
    15481 aaagtttatt atcaacaacg cttgggcaac taccaaatgc agctcaaaaa actgcacagc
    15541 gacgcacaag ccgcatttaa tgccgtccct gctgccaaac gcaaagtcct gaccgggcac
    15601 gatgcctttt cctatatggg caaacgttac catatcgaat tcatcgcccc gcaaggcgtg
    15661 agcagcgaag ccgagccttc ggccaaacaa gtcgccgcca tcatccgcca aatcaaacgc
    15721 gaaggcatca aagccgtatt taccgaaaac atcaaagaca cccgtatggt tgaccgcatc
    15781 gccaaagaaa ccggcgtcaa cgtcagcggc aaactgtatt ccgacgcact cggcaacgcg
    15841 cccgcagaca cctacatcgg aatgtaccgc cacaacatca aagccttgac caacgcgatg
    15901 aagcaataaa accgtttgat tggtttgaat ttgaaaaggc aaaaggccgt ctga
//
LOCUS       DAWFJW010000038        15752 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFJW010000038 DAWFJW010000000
VERSION     DAWFJW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.038"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WGR19_000989"
     CDS             complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WGR19_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0798538.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WGR19_000990"
     CDS             complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WGR19_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0798539.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WGR19_000991"
     CDS             complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WGR19_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0798540.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3427..7602)
                     /gene="rpoC"
                     /locus_tag="WGR19_000992"
     CDS             complement(3427..7602)
                     /gene="rpoC"
                     /locus_tag="WGR19_000992"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0798541.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(7754..11932)
                     /gene="rpoB"
                     /locus_tag="WGR19_000993"
     CDS             complement(7754..11932)
                     /gene="rpoB"
                     /locus_tag="WGR19_000993"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0798542.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKASELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(12122..12493)
                     /gene="rplL"
                     /locus_tag="WGR19_000994"
     CDS             complement(12122..12493)
                     /gene="rplL"
                     /locus_tag="WGR19_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0798543.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(12551..13051)
                     /gene="rplJ"
                     /locus_tag="WGR19_000995"
     CDS             complement(12551..13051)
                     /gene="rplJ"
                     /locus_tag="WGR19_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0798544.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(13281..13976)
                     /gene="rplA"
                     /locus_tag="WGR19_000996"
     CDS             complement(13281..13976)
                     /gene="rplA"
                     /locus_tag="WGR19_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0798545.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNIAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(13976..14410)
                     /gene="rplK"
                     /locus_tag="WGR19_000997"
     CDS             complement(13976..14410)
                     /gene="rplK"
                     /locus_tag="WGR19_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0798546.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(14511..15047)
                     /gene="nusG"
                     /locus_tag="WGR19_000998"
     CDS             complement(14511..15047)
                     /gene="nusG"
                     /locus_tag="WGR19_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0798547.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(15049..15327)
                     /gene="secE"
                     /locus_tag="WGR19_000999"
     CDS             complement(15049..15327)
                     /gene="secE"
                     /locus_tag="WGR19_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0798548.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(15443..15518)
                     /locus_tag="WGR19_001000"
     tRNA            complement(15443..15518)
                     /locus_tag="WGR19_001000"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15483..15485),aa:Trp,seq:cca)
     gene            complement(15525..>15752)
                     /locus_tag="WGR19_001001"
     CDS             complement(15525..>15752)
                     /locus_tag="WGR19_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0798549.1"
                     /translation="HTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVEL
                     IAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         3964 a         4080 c         3511 g         4197 t
ORIGIN      
        1 gctaattcct ttacattaaa gatcgattaa agaacaatgc cgtctgaaaa atcatttcag
       61 cctttcagac gacctatttg cctgattagc ctttacgggc ttcagttaca gcagcagcta
      121 tgtgggcagg agcttcagaa tatttcttga actccataga gtaagtagcg cggccttggg
      181 ttgcagaacg caggtcggtc gaataaccga acatttctgc cagaggtact tcggcacgga
      241 cttttttacc gccgataccg tcatcatcca tacccaatac aacaccgcga cggcggttca
      301 agtcgcccat tacgtcgccc atgtattctt ccggagtttc cacttcaact gccataatcg
      361 gctcaagcag ggcaggagat gcttgacgca taccttcttt aaacgcttga gaagcggcca
      421 attcaaatgc caattgggaa gagtcgacat catggtaaga accgaatacc agacggatac
      481 gcacatcaac tacaggatag ccggcaacga taccgttagg caatgtatcg cggatacctt
      541 tatcgacaga cggaatgaat tcgcgaggaa tcacgccacc tttaatttca tcgataaact
      601 cgtaaccttc gccccccggt tccataggtt ccatttcaat cacaacgtga ccatattgac
      661 ctttaccacc ggattgtttc gcgtgtttgt attcagcttt aacggctttg cggatagttt
      721 cacggtaagc cacttgaggt gcaccgatat ttgcttccac accgaattcg cgtttcatac
      781 ggtcaacaat aatttccaag tgcagctcac ccataccgga aataatggtt tgaccggatt
      841 cttcgtctgt acggacacgg aaagaagggt cttctttagc caagcggttc agggcgatac
      901 ccattttctc ttggtcggct ttggttttcg gctcaacggc aatatggatt accggctcgg
      961 ggaattccat acgttccaag ataatcggcg cgctttccgc acacaaggtt tcaccggtag
     1021 taacgtcttt cagaccaata gcggctgcga tgtcgccggc gcgtacttct tcgatttcag
     1081 tacggtctgc ggcagtcatt tgtaccaaac gaccgatacg ttcgcgagtg ccttttacgg
     1141 agttcaatac ggtatcgccg gattttacta cgcctgagta aacgcggata aaggtcagct
     1201 gaccgacgta tttgtcgttc aacattttga acgccaatgc agagaatttc tcttcatcgc
     1261 tggcttgacg gctgtcggct tcctcggtat tcgggttgac accttgaacc ggaggaatat
     1321 cggtaggagc tggcagcaat tctacaactg cgtccaacat acgttgaaca cctttgtttt
     1381 taaatgcaga accacacagc ataggctgaa tttcgcctgc caaagtacgt tgacgcaacg
     1441 cgcctacgat ttcttcttcg gtcagctcgt cgccgcctaa gtatttgtcc atcagttctt
     1501 cgctggcttc ggctgcggct tcaatcatat tttgacgcca ttcttcggca gtttcgacca
     1561 attcggcagg aatatcgcca taggtaaagg ttgtaccttt atcggcttca ttccaaatga
     1621 tggatttcat tttcaacaaa tcaaccacac cgctgaagtt gtcttccgca ccaaccggaa
     1681 tgacgatagg tacagggttt gcgcgcaaac gggttttcat ttgctcgaca acgcggaaga
     1741 agttggcacc ctgacggtcc attttattga caaacgccaa gcgcggcact tggtatttgt
     1801 tggcttgccg ccatacggtt tcagattggg gttgaacacc gcccaccgcg cagtaaacca
     1861 ttaccgcgcc gtccaataca cgcatagaac gctctacctc tacggtaaag tcaacgtgtc
     1921 ccggggtgtc gatgatgttg aagcggtgct cggggaattg tttcgccata ccggaccagt
     1981 aggaagtaac ggcagcggag gtaatggtaa taccgcgctc ttgctcttgt tccatgtagt
     2041 cggtagtagc cgcaccgtca tgcacttcgc ccagcttgtg ggtcaaacca gtatagaaca
     2101 aaatacgttc tgtcgtcgtg gttttacccg catcgatatg ggcggaaata ccgatgttac
     2161 ggtacaggct gatcggggtc ttacgagcca ttttattagc ctttcaaaat tagaaacgga
     2221 agtgagagaa tgctttgttg gcttcagcca tacggtgtac ttcttcacgt tttttcaacg
     2281 caccgccacg accttcggac gcatcaatca attcgcctgc caaacgcagg tccatggatt
     2341 tctcaccacg tttgcgggcc gcatcgcgaa cccagcgcat tgccaaagcc aaacggcgtg
     2401 aaggacgaac ttcaacagga acttggtagt ttgcaccacc tacacggcgg cttttcactt
     2461 ccacgatagg cttggcgttt gcaatggctt cgttaaatac ttcgattgct actttgccgg
     2521 tttttttctc aatctgttcc aacgcaccgt aaacgatacg ctcggcaacg gattttttac
     2581 cgtcaatcat caatacgttc atgaatttgg tcaactcgac gctgccgaat ttaggatctg
     2641 gcagtacgtc gcgcttgggg acttctctac gtcttggcat tttaatttcc tttaatctat
     2701 tcagtcgggt ctattcccat gaatacccaa ttgagtattc acttactcgg ccgttgttca
     2761 gcttaggcgg ccgacgtgcc tatttaagtc ccagtaatta tttaggacgc ttagcaccgt
     2821 atttggaacg ggcttgttta cggtctttaa cgcctgcagt atccaaagaa ccgcgtacag
     2881 tgtggtaacg cacacctggc aagtctttta cacgaccgcc gcgaatcaat acgacactgt
     2941 gctcttgcag gttgtggcct tcgccgccga tgtatgaaat gacttcaaaa ccgttggtca
     3001 ggcggacttt acatacttta cgcaatgcag agttaggttt tttaggggta gtcgtgtata
     3061 cacgggtgca cacgccgcgt ttttgcgggc aggcttccag tgcgggcact ttgtttacgt
     3121 acacgggctt ttgacggcct ttgcgtacca attggttgat agttggcata ttttctcgtc
     3181 ctgttgagtt aaatacttgc cgacaccatg tcggcaagag tggaattata tttttattgc
     3241 aagcagacag tcaagcgtga gatcaggaag attcatcatt tttctttttt cagataaaca
     3301 tttaggtccc ttatgctcat aaacaactat taccggaaag gaaatcacta ataatttaaa
     3361 tgccgtctga aagaaaaacc acaagcctaa agcttgcggt tttgtattga ataaacgcgc
     3421 cgaagattat tcatccgtta cttgggtttc agcagtctcc tgctctacct cttgccattg
     3481 ctgatggcga ctgcggtggt aagtcaaacc ggtaccggca ggaatcaggc ggccaacgat
     3541 gacgttctct ttcaaaccgc gcagttcgtc ctgtttgccc ataatcgcgg cttcggtcag
     3601 gacgcgcgtg gtttcttgga acgatgcagc agaaatgaag ctgtcggtag acagggaggc
     3661 tttggtaata cccaacaata cgttttcgta acgtgccggc tctttacctt cctccaacgc
     3721 tttttcattg gccgccatca catcgccgcg ttcgacctgc tctccggtaa tgaacccggt
     3781 ttcgccggca tccgcaatgt tcacacggcg caacatttga cggatgatga cttcgatgtg
     3841 tttatcagaa atcttcacac cttgcagacg gtaaacctct tgcacctctt ggacaatgta
     3901 gcgtgccagt gcttcgatac cttgcaaacg cagaatatca tgcggatcga ccgcgccgtc
     3961 cacaatggtt tcgcctcggt ttaccacttg accgtcgtgt accagaattt gtttctcttt
     4021 ggaaatcaaa gtctcgtatg ctacgccgtc cacgtcggta ataatcagac gttgtttgcc
     4081 tttggtctcc ttaccgaagg aaacggtacc ggtaatttcc gccagcatac cggcatcttt
     4141 cggcacgcgt gcttcaaaca attcggcaac gcgcggcaga ccgccggtaa tgtcgcgggt
     4201 tttggaagag gcttgcggga tacgcgccaa tacgtcgcct ttaccgattt cctgaccttc
     4261 gcgtacggta atcaccgcac caacggggaa tgccatcgaa accggagtgg acgtaccggg
     4321 aatacagatt tccacgccgt tttcatccaa caattttaca gtcggacgca gcagtttgga
     4381 cgcgctgctg gaacggcgtt taccgtcaat caccaccaaa gtggacaaac cggttacatc
     4441 gtcagtttgt ttggcaacgg ttacaccctc ttccacgttt tcgaatttca ccatacctgc
     4501 gtgttcggta atcatcggac gggtatgcgg atcccaggtt gccaaggttt gaccggcttt
     4561 aatggccata ccgtcttgta ccagcaggat ggcaccgtaa ggtactttgt ggcgttcgcg
     4621 ttcacggccg atatcgtcgt gaatcacgac ttcacaagaa cggccgatga caaccaactc
     4681 gcctttgttg ttggcaacgt agcgcatctg gctgctgaat cgtgccgtac cgttggattt
     4741 ggcttccact tggctggctg ctgccgcacg ggatgccgca ccaccgatgt ggaacgtacg
     4801 catggtcaac tgggtacccg gttcgccaat ggattgtgca gcaatcacac cgactgcctc
     4861 accggcgtta accagtttgc cgcgtgccaa gtcacgaccg taacagtgtg cacacaggcc
     4921 gtgacgggtt ttacaagtaa tcggcgtacg gactttgact tcatcgacac cggattggtc
     4981 gatcatatcc accagttttt cagtcagcaa cgtaccggct tcaaccaagg tttcgccact
     5041 tgacggatcg acaacgtcag acgcggtaac acgacccaaa atacgatcgc gcaatgcttc
     5101 aatcacatca ccgccttgta ccactgcctt catgacaaag ccgtctgaag taccgcaatc
     5161 gtcttcaaca acgaccaaat cttgagttac gtctaccaga cgacgagtca ggtaaccgga
     5221 gttcgcggtt ttcaatgcgg tatccgccaa acccttacgc gcaccgtggg tcgcaataaa
     5281 gtattgcaat acggtcagac cttcacggaa gtttgaggta atcggcgttt caataatcga
     5341 gccgtcaggt tttgccatca agccacgcat accggacaac tgtttaatct gagctgcaga
     5401 accacgggca ccggagtccg ccatcatata aatggagttg aatgactctt gatcgacttc
     5461 gttgccgtca cggtcgataa ctttttgttt ggacaagtta tccatcatcg ctttagcgat
     5521 tttatcgccg gcacgacccc aaatatccac tactttgttg taacgttcgc cattggtaac
     5581 caaaccttga cggtattggt cttcgatttc tttaacctcg gcattggctt cagccagcaa
     5641 ggccgctttt tctttcggga tttccatatc gtcaacggca atggaaatac cgccttttgc
     5701 cgcaaatccg aaaccggtgt acatcaggtg gtcagcaaag ataaccgtat cgcgcaagcc
     5761 gcacaggcgg aacgatgcgt taatcagttt agaaatttct tttttcttca atgccttgtt
     5821 gacatattca aacggcaggc ctttcggcag gatttcgctc aacaatgcac ggccgacggt
     5881 tgtttcgtaa cggttaacga caggctcaaa ctcacctgct tcgtttttca cccattcgcg
     5941 cagacgtacg gtgatttttg tacccagctc gacctgtttg gtatggtatg cgcgatgcac
     6001 ttctttcaca tcggcaaaca ggctgccttc gcctttggca ttgatacggt cgcgggtcat
     6061 gtagtacaga cccaatacga tatcttggga aggtacaata atcggttcgc cgttggcagg
     6121 agacaatacg ttgtttgaag ccagcatcag cgtgcgtgct tccatttgtg cttccaagct
     6181 caatggaacg tgtaccgcca tttggtcgcc gtcaaagtcg gcgttgaacg cggcacacac
     6241 caatgggtgc aactgaatcg ctttaccttc aatcaagata ggttcgaacg cttgaatacc
     6301 caaacggtgc agggtcggcg cacggttcag cataatcgga tgttcgcgga tgacttcttc
     6361 caagatgtcc catacttccg gtacttcttg ctctaccaat tttttcgctg ctttaacggt
     6421 agaggccaaa ccttgttttt ccaatttgtg gaaaatgaac ggtttgaaca gttccaaagc
     6481 catttttttc ggcagaccgc attggtgcag acgcaggtat gggcctacgg taatcacgga
     6541 acggccggag tagtccacac gtttgcccaa caggttttga cggaagcgac cgcctttacc
     6601 tttaatcatg tctgccaatg atttcagcgg gcgtttgttg gcgccggtca tggctttacc
     6661 gcgacggccg ttatccaaca gcgagtcaac tgcttcttgc aacatacgtt tttcgttgcg
     6721 gacgatgatg tcaggcgcgt gcagttccaa cagacgtttc agacggttgt tacggttaat
     6781 aacgcggcgg tacaaatcgt tcaaatcgga agtggcaaaa cgaccaccat ccaatggaac
     6841 caacggacgc aaatcaggcg gcaataccgg cagcacatcc ataatcatcc attccagttt
     6901 cataccggaa cgatggaagg cttccaatac tttcaagcgt ttggcgattt ttttgatttt
     6961 ggtgtcggaa ccagtcgatt ccaactcttg gcgcaggatt tcgatttcgc ccgctacatt
     7021 cagggtacgc aacaattcgc ggataccttc tgcacccatt ttggcatcga agtcgtcgcc
     7081 gtattcgtcc agcttgttgt agtaatcgtc ttcagtcagc aattggcggc gttgcagcgg
     7141 agtcataccg ggatcggtta ccacaaatgc ttcaaagtac aatacgcgct cgatgtcgcg
     7201 caaagtcatg tctaacacca tacccaagcg ggaaggcagg gattttaaga accaaatatg
     7261 tgcgacgggc gcagccaatt cgatatgacc catgcgttcg cggcgcactt tggacagggt
     7321 tacttccacg ccgcattttt cacacgttac gcctttaaat ttcaagcgtt tgtattttcc
     7381 gcacaagcat tcgtagtctt tgaccgggcc aaagattttg gcacagaaca aaccgtcgcg
     7441 ctcaggtttg aacgtacggt agttgatggt ttcaggtttt ttgacttcgc cataagacca
     7501 tgagcggatg gtttcgggag aggcaatacc gattttaatg gcatcaaact cttcttccat
     7561 gccggcagtt tgcaacggat taaataagtt caacaaattc atttttgctc cttgaaggaa
     7621 gtatttttac cgcatggcgg attttcgata tggatagagg tacagtgttt tcagacggca
     7681 tttcgttttt aaacattgtc ggctgccccg catacctgca gaacagctcc ccttatgccg
     7741 tctgaaaccc gtatcagtag cgttccaaat cgatatccaa gcccagtgag cgaatctctt
     7801 tgaccaatac gttgaaggac tcgggcatac cggcatcgat tttgtgttcg cctttgacga
     7861 tgttttcgta cattttggta cgaccgttca cgtcgtcaga cttcacagtc aacatctctt
     7921 gcagcgtgta tgccgcgccg tatgcttcca atgcccaaac ctccatctcg ccgaaacgtt
     7981 ggccgccaaa ctgggcttta ccgcccaaag gctgctgggt aaccagactg tacggaccgg
     8041 tagaacgggc gtgcattttt tcgtcaacca agtggtgcag tttcagatag tgcatcacac
     8101 ctactgtaac cttgcggtca aatgcttcgc ctgaacggcc gtcatacagc gtgatttggg
     8161 ttttactgtc gttgaagccc agtttttcaa cctcaggatc gtcgctcgga taagccaagt
     8221 tcagcatttc gcggatttca gactctttcg caccgtcgaa tacaggagag gcgaaagatg
     8281 cacctttgcg caggttggag gccagttcga tgatttcttc atcagtcagg gcatccaaat
     8341 cttctttctt accgctgccg ttgtagagtt tgttcaagaa ctcgcgcaac tcgctggctt
     8401 tgcgttgctc tttcagcata cggtcgatac gctcgccgat accttttgct gcccaaccca
     8461 agtgaacttc caaaatctga ccgatgttca tacgggaagg tacgcccaat gggttcagta
     8521 cgatgtctac cggacggccg tccgccatgt aaggcatgtc ttccactggc agaatgcgcg
     8581 ataccacgcc tttgttaccg tggcggcccg ccattttgtc gccggcttgc agacggcgtt
     8641 tgatggcgat aaaaactttc accatttttt gtacgccggg ttgcaattcg tcgccttggg
     8701 tcagtttttt cttcttgatt tcgtataact cgtccgcttc ttcgcgtttt tgttgcaggc
     8761 tcactttaat cagttccaac tgcttggcca aatcttcatc ggtcagacgg atatcgaacc
     8821 aatcgtgcct gctcggcaga cccgccagat attcggtcgt gatttcactg cctttggcca
     8881 gcttcatcgg gccgccgttg gctttctgac cgacaatcat acgctcgata cggtcgaatg
     8941 cgtcgttgtc gaaaatacgc aattggtcgt tcaaatccaa acggtagcgt ttcaactcgg
     9001 aatcgataat ggattgggcg cgtttgtcgc gttgaatacc ttcacgagtg aagacttgaa
     9061 cgtcgataac cgtaccgctc ataccggtag gcatacgcaa tgaagtatct tttacgtcag
     9121 acgctttttc gccgaagatg gcgcgcagca gtttttcttc aggagtcagt tgggtttctc
     9181 ctttgggcgt tactttacct accagcacat cgccggcttc tacttctgca ccgatatata
     9241 cgataccgga ttcatccaaa cggttttgca tacgttcgga caagttcgga atatcgcggg
     9301 taatgtcttc cgcgcccagc ttggtatcgc gggcaacgac attcaattcc tcaatgtgaa
     9361 tcgaagtata gcggtcgtcc gcagccactt tttcggaaat cagaatcgag tcttcgtagt
     9421 tgtaaccgtt ccacggcatg aaggcgatgg tcatattttg acccaaagcc aattcgccca
     9481 aatcggtgga cgcgccgtcg gccaccaaat cgccgcgctg caacacatcg ccggctttga
     9541 cggccggacg ttggttgatg ttggtagatt ggttggaacg ggtgaatttg accaagttgt
     9601 aaatatcgac acccacttcg ccggcagtcg cttcgtcgtc atggacacgg atcacaacgc
     9661 ggttggcatc gacatactcg accacgccgc ctcggcgggc aacgattgca gtagcagagt
     9721 caacggcaac ggaacgctcg ataccggtac cgaccatcgg tttttcagga cgcaggcaag
     9781 gcactgcctg acgttgcatg ttggcaccca tcaatgcgcg gttcgcgtca tcatgttcca
     9841 agaacggaat cagggatgcc gcaacggata ccacctgacc ggttgccacg tccatatatt
     9901 ggacgcggtc gggcgttgcc ataatggttt cgcctttttc acgacaggta accaaatcgc
     9961 caatcagatt gccatctgaa tccaaatcgg cattcgcctg tgcaatcaca tagcggcctt
    10021 cttcgatggc agacaagtaa tcgatttcct cggttacttt gccgtcgata acgcggcggt
    10081 aaggcgtttc caagaaaccg taatcattgg tgcgcgcata aacggacaat gagttgatca
    10141 aaccgatgtt cggaccttca ggcgtttcaa tcggacatac gcgaccgtag tgggtcggat
    10201 gcacgtcccg cacctcaaat cctgcacgtt cgcgggtcaa accgcccgga cccaatgcgg
    10261 atacgcggcg tttatgggtt acttcagaca agggattggt ctgatccata aactgactca
    10321 attggctgga gccgaagaat tctttaatag cagcagaaac aggttttgca ttaatcaaat
    10381 cgtgcggcat caagttttct gattccgcct gattcaaacg ttcttttacg gcacgttcca
    10441 cacgggccaa accgctacgg aattggtttt cagtcagctc gcctaccgaa cgtactcggc
    10501 ggttgcccaa gtgatcgata tcatccactt cgccatggcc gttacgcaac tcgaccaaag
    10561 tcgcaatcga ggcgacaata tcttcgacgc tcaggacata accgcctttg tcggcagcac
    10621 cggcaaacgt ttcgttcagc aggcggccgt accaagagtt ttgttgggct tcggacagtt
    10681 tttgttcgta tgtgcgcgta ttaaatttca tacggcctac gcgggacaaa tcgtagctgt
    10741 cttcactgaa gaacaagcgg ttaaacaatt gctcgaccgc ctcttcagtg ggcggttcgc
    10801 ccggacgcat catacggtaa atcgcaacac gcgccgcctg ccggccggca gtctcatccg
    10861 tacgcaaggt attggagata taagcaccct gatccaactc attgatgtaa agggtcgtaa
    10921 tttcttttac gccgttgata tcaaatttgg ccaacaactc ttctgtaatt tcatcattgg
    10981 cagaagccaa tacctcgccg gtttccgaat caatcagatc ggcagccaat gctttgccca
    11041 gcaggctttc tggttctaca tccaaacggg tcaggcctgc attggtaata tcacggatat
    11101 ttttcgcagt aatgcgctta cctttggcaa ccaatacatt gccttcttta tccaagatat
    11161 cgaccttggc agtttcgcct ttcagacggc ctgcgaccaa atcggtttga acaccgtttg
    11221 aagacaaata gaacgtttct ttgtcgtaga aaatatccaa gatttgctca ttgttgtagc
    11281 ccaaagcctt caacaaaatc gttaccggca ttttacggcg gcggtcgata cggaaataca
    11341 gcaaatcttt cggatcaaat tcaaaatcca accatgaacc acggtaggga atgatgcggg
    11401 cggagaataa caatttgccg gaagagtgcg tcttaccttt gtcatgctcg aagaatacgc
    11461 cgggcgaacg gtgcaactgg gagacaatca cacgctctgt gccgttaatc acaaaagaac
    11521 cgctcggggt catcaacgga atttcgccca tatacacttc gttttcacga acttctttta
    11581 ccgtcggttt agatgcttcc ttatccaaaa tcaccaaacg gatacgcgcg cgcaaggggg
    11641 ctgcataagt gattccgcgc aactgacatt cgggaatatc gaacaaaggc tcgcccaatg
    11701 tgtaatgcac aaactccaat cgcgcataac cgttatggct cacaatcggg aaaatagaat
    11761 taaatgccgc ctgcagaccg tcatcggtac gtttgtcaaa agcattttcc agctgcaaaa
    11821 acttcgcata agaatcaatt tgggttgcta gcaagaaagg aacttccaaa acattttccc
    11881 gctttgcaaa actcttacgg atacgttttt tctcggtaaa cgaatagttc atacacactc
    11941 cgaaaagtaa ttttcaataa taggccgtct gaaacaacgg tacgcatcaa tctgatattt
    12001 acatttattt acaattactt gataaaaact atgcaaataa atgtaaacaa tacttgatgt
    12061 ttattttaag aagcaaaata aggctggcag aaaactgcca gccttcataa gaagcatcaa
    12121 attatttgat ttcgacttta gcgcctgctt cttccagttg tttttggatg tcttcggctt
    12181 cagctttaga aacaccctct ttaatggttt taggtgcgcc gtcaacgatg tctttagctt
    12241 ctttcagacc caaaccggta attgcacgga caactttaat cacgccgact ttttgatcac
    12301 cggcagaagc caaaacgaca tcaaattcgg ttttttcttc agcatcggca gcaccggcac
    12361 cagcaggacc tgcaactgca acagcagcag cagaaacacc gaatttttct tcaaaagctt
    12421 taaccaagtc gttcaattcc attacggtca aagaaccaac tgcttccaaa atgtcttctt
    12481 tagtaatagc catgctattt tactccaaat attgtattaa aaaataattg attaacagaa
    12541 acaaaatcga ttaagcggct tcttcgccgg cttttttctc tgccaaagca gccaaaccgc
    12601 gcgcaaagcc cgatacagga gcttgcataa cgaacaacag tttggacaac agctcttcgc
    12661 ggctcggaat agaagccaac tcagcaacct gagcagcatt cattacttcg ccattgtaag
    12721 aaccggcttt aacgacaatt ttgtcatctt ttttcgcgaa ttggtgcaac actttagcag
    12781 cagcaacagc atcttcagaa gcagcgtaaa ccaacggacc aaccatttga tcggccaatt
    12841 ctgcaaatga agtaccttgc actgcacgac gagccaaagt atttttcaga acgcgcaaat
    12901 aaacgccttc tttacgtgca ttcgcacgaa gctcagtcat actggaaaca ctgataccgc
    12961 gatattcagc gactacgagg gtttgagcat tagcaattgc cgcgctaatt tcctcgaccg
    13021 ccactttctt ggtttcaata ttgagactca aggtctacct cccactgttt ataaacagga
    13081 taccaaaacg atatcccacc agcgcggtaa cctaaaaaga catcttacaa gcaatcttgc
    13141 aatgtgtttc aggactaccg tctgcgtagg gcagttacga ttaaatctta cgatccctac
    13201 ggtcttggac atctacttaa ttttaagtag cccaaattca agcagcctag aaaaccaaac
    13261 tgcttgaaaa ttccttaaga ttagttattt acgcttgatg tatcaacgcg aatacccaaa
    13321 cccatggtgc tagacacagc aacttttttc agatactgac ctttagcggc agcaggcttg
    13381 gctttaacga tagcatccag caacgcatca aagttctctt tcaaatcagc ttcagcgaat
    13441 gaagcacgac caatcgttgc atgaacaata cctgccttgt cggtacggta ctgcacttga
    13501 cctgcttttg cattttttac tgcttcggca atattagggg taaccgtacc tactttaggg
    13561 tttggcatca gaccacgagg acccaagatg gtacccaatt gaccaacaat acgcattgca
    13621 tcgggagaag caataacaac gtcaaagttc agattgcctg ctttgatttc agcagccaaa
    13681 tcttcgaaac cgacgatatc tgcaccagct tctttagcag cttctgcatt tgcaccttga
    13741 gtaaatacag ccacacgggt tatcttaccg gtgcctttag gcagaacgac cgaaccacgg
    13801 ataacttggt cagatttacg cggatcaacg cccaagttga aagatacgtc aacagactcg
    13861 tcaaatttag cagtcgctgc tttttttacc aaagcaattg cttcatcaat tgcatataat
    13921 ttattggctt ccacagaaga gcgaagagct ttcaaacgtt tagatacttt agccattata
    13981 caacaccctc cacatccaag cccattgagc gagcagaacc tgctatagta cggacagccg
    14041 catccaagtc agcagcagtc aaatcaggat ctttagtttt agcaatttct tccaactggg
    14101 cacgggtcaa tttacccact ttgttggtca gtggattaga actacctttt tgcaaaccgg
    14161 cagccttttt caacaagata gaagctggcg gggttttcat cacaaatgtg aatgatttat
    14221 ctgcaaatgc agtaatcaca accggaatcg gtaagccagg ctccatacct tgggttgcag
    14281 cattaaatgc cttacaaaat tccataatat tcaaaccgcg ctgacccaaa gcaggaccaa
    14341 ccggaggaga tggattggct ttacctgcag gaatttgcag tttaatatag ccgataattt
    14401 tctttgccac ttaacgaact cctaaaaacg ggtaagacgc ggaaccattt ccgctcccca
    14461 agttaatttt tgattctatc ctcttattgc ttttttttca agtataaaaa tcagttaatc
    14521 ttttcaacct ggctgaactc cagctcaacg ggtgtttctc taccaaatat ctgaacagac
    14581 acgcgtaact tattccgttc ataattgacc tcctcaacca ccccgttaaa atccgcaaac
    14641 ggcccttcat ttacacgaac ctgttgaccg acctcaaatt caacttttgg tttcggcttc
    14701 tctatgccgg tctgaacctg ctgtaaaata atttcagcct ctctctgact aatcggcgta
    14761 ggtctattag ccctccctcc aataaaaccg gaaacacggg gggtgctttt tacaagatgc
    14821 caagagtcat ctgtcatttc catctcaact agcacataac caggatatga ctttctttca
    14881 ctaatagtct tacgaccatt gcggatatca acaactttct ctacaggcac cagaatttgt
    14941 ccgaaataat ctcccatctc ctcacgggca atgcgctctt ccaatattcg ttggacattc
    15001 ttctcaaacc ccgaatacgc ctgtacaaca taccattttt tcgacatctc aaccttccct
    15061 tctcagcaat acatcaaaaa ataaccacga aattgctgta tctgccgcat agataaatat
    15121 agaaagcaca gcaacaaaca ctataacaaa tacagtcatt ctgacagcat cttcacgctt
    15181 aggccaaacc acctttttga attcggacca agaatttgag aaatatgcaa aaaacccttc
    15241 cttaccggaa ttagatgcag attctttatc ttgaacaacc agttgatcca ctttaacgtt
    15301 ctttttttca ggcgtatgtt ctgtcatatt atttatccat catagcatct gtctattctc
    15361 aatccatgta aatggcaaga gagtttacta aataacaaat acaaaaaaaa ttaaccggca
    15421 caaggccggt taatttttta tttggcaggc caagagggtc tcgaaccccc aaccctcggt
    15481 tttggagacc gatactctac caattgagct attggcctct aaacttaagc gataacagaa
    15541 gaaaccacgc cggcacccac ggtacggccg ccttcgcgaa tcgcaaagcg caaaccttct
    15601 tccatagcga taggcgcaat cagttctacg gtgatggtta cgttttcacc cggcattacc
    15661 atttccacac cttcttccaa agtaaccgcg ccggttacgt cggtggtacg gaagtagaat
    15721 tgcggacggt agttggcgaa gaatggagtg tg
//
LOCUS       DAWFJW010000039        15422 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFJW010000039 DAWFJW010000000
VERSION     DAWFJW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.039"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(83..3172)
                     /locus_tag="WGR19_001002"
     CDS             complement(83..3172)
                     /locus_tag="WGR19_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0798550.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALLGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGMTVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILPCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEYAAGLSGEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDKCMSDPFALDFI
                     CQGSWGDPGVDADKAEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRYRVRPNT
                     GATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIRADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPG
                     AGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAAEVNAER
                     LAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYG
                     LIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            3446..4225
                     /locus_tag="WGR19_001003"
     CDS             3446..4225
                     /locus_tag="WGR19_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0798551.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGNTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(4435..5847)
                     /locus_tag="WGR19_001004"
     CDS             complement(4435..5847)
                     /locus_tag="WGR19_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0798552.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGRLHAHKVKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGILNAKTADLKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            5938..6014
                     /locus_tag="WGR19_001005"
     tRNA            5938..6014
                     /locus_tag="WGR19_001005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5972..5974,aa:Arg,seq:acg)
     gene            6032..6106
                     /locus_tag="WGR19_001006"
     tRNA            6032..6106
                     /locus_tag="WGR19_001006"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6065..6067,aa:Glu,seq:ttc)
     gene            6665..7840
                     /locus_tag="WGR19_001007"
     CDS             6665..7840
                     /locus_tag="WGR19_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0798553.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(8181..8729)
                     /locus_tag="WGR19_001008"
     CDS             complement(8181..8729)
                     /locus_tag="WGR19_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0798554.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(8732..10228)
                     /gene="ccoG"
                     /locus_tag="WGR19_001009"
     CDS             complement(8732..10228)
                     /gene="ccoG"
                     /locus_tag="WGR19_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0798555.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAALKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            10756..12735
                     /gene="tkt"
                     /locus_tag="WGR19_001010"
     CDS             10756..12735
                     /gene="tkt"
                     /locus_tag="WGR19_001010"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0798556.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            12876..14264
                     /gene="fumC"
                     /locus_tag="WGR19_001011"
     CDS             12876..14264
                     /gene="fumC"
                     /locus_tag="WGR19_001011"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0798557.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHDLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIHLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(14330..15253)
                     /locus_tag="WGR19_001012"
     CDS             complement(14330..15253)
                     /locus_tag="WGR19_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0798558.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         3891 a         4412 c         3540 g         3579 t
ORIGIN      
        1 gtcccgatag acctgcctcc ataaaaatgc cgtctgaaac ataccctgtt tcagacggca
       61 tccgcaaaac tacggttttc aattaaaact gccaatccag tttcatgctg acagtgcgcg
      121 gctctccata gaagttgttt gcgccgcgcg tacggttgta gttgttctca aaataagtgc
      181 gtccgtttaa gttcgtaccg atgaggctca atttggcgtg tttgcccaat tcgtaacgga
      241 cgaaaccgtc tatcagcccg tagccgccct gcctgatgtt atacagactg cttgtgccgc
      301 tttgtgcgga cacgccgccg ccgacggtca gccccgtatt cggtatatgg aagctcgttc
      361 cgaaacggaa tatgtgcacg ggtgtgaaat tgctgaagtt gtacgggtct gcgcctgtgt
      421 ttttggcgag gcgttcggcg ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg
      481 tgtaacccgc aaagactttc caatcttcgt tcaactcacc cgacaactcg aattccgcac
      541 ccctgctgac cactttgcct atcggtttgg caacggtttg gaacgacccc tgcttgccgc
      601 ctgctccggg aacatagcca aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca
      661 atgcgaacga agcattcagc cgtccttgca agaacgcgcc tttccagcct acctcatagt
      721 ttgtgccgac caaaggcggt aaaacggttt tggcactgac atcgacatta tcctgctgtt
      781 tgaagatttt ggtataactt ccgtaaatac tctgttgcgg tgtcaagtca taggtaatgc
      841 ctgcataggg cgtcaattta tgaccttgca tcttggccgt gtaatggtcc tgatccgccc
      901 taatgctcga tgccgtctga aaatcgcttg ccggctgccc atagcggaca ggcatatctt
      961 tggtttgcga agtctcatag cgcgtgtagt gcagcccgcc caaaaggtgc agtcggccgg
     1021 ttacgttgaa acgcgtgctg gcagtcagcg aatgggtttt gttggtgttg aggtatttgg
     1081 cgtagttata cagcgcagga acatggtcgt ctgccacttt gacggttttc caaaccggca
     1141 ccgtaccgga aaaaccggta aaggcaggcg tgccgtcggg attggtctcc tgaatcttgt
     1201 tgcctttttc gtccagctca tatacatcga catataccgg tgtccggctg ccgctgtatt
     1261 cgtcatagta atacacctgc ttgccttcgg catcgagctt gggctcggtt tttattttct
     1321 tggcgttcct gcattcttcg gcataaacgg tacggttgcc tttttcatcg tacgcctgcc
     1381 aatcgggttc tttatgcccc ctgaccaaag gagacgacaa atcgccgtcc ggctcctcct
     1441 gacaacttcc cgcatacacg ccgtgcgttg cccccgtatt cggacgtact ctgtagcggc
     1501 gttcgtagat ttctagatat tccgaacgta tcttttcatc accgtaggca tagccgacaa
     1561 agaaatcatg ctcccgcccg aacagcccat atgtgccggt caggtcaagt ttaattcccc
     1621 attggcggtc gtctttggta tgccgcaacg gcatataact gtatcgtcgg ttggcggtag
     1681 ccttccgatt aaaggaagag tcatacaggc tgtttggaaa acgttgtgcc gcattattaa
     1741 agatgccctc cttcgcaagg gctttatcga caaattccgc cttgtcggca tcaacgcccg
     1801 gatcccccca agaaccttga cagataaagt ccagcgcgaa agggtcactc atacacttgt
     1861 caaaaccggc tttgcgttct gcggcacggc ggctgcgata ctgttcgaaa gcagtattat
     1921 cgaaacggtt tttaacaaaa tcgtctttgc gctcccggta ttccttggcg gtttcatcac
     1981 gatatgcttt cagtttctcc aatgccttat ctttcggctc gaacgggatg acttcgtttt
     2041 tttcagtcaa aaagcctacc gcatcctcac ccgacaaacc cgccgcatat tcgtttttca
     2101 gaaaaaactg ccccaccttc gcatcggatt cattcttggt ataagacact tcggcattga
     2161 gctgccaacc gttgtcaaac acatgtttga atcctgagaa aaggttgtat ttgtcggcac
     2221 ttaaccgcga ccaatcctcc cccaaataag tgttgcgcgg cagttgcaaa ggccggttgc
     2281 aggcaggcgt tgaactgaac ggggcagttt tctgattttc acagggcaaa ataatgcccg
     2341 aaaaatcagg aacctcccta ctcttctgat acatgccgcc caaagtaagc acactgctgt
     2401 cgcccgcatc ggcttcggca atgccgtaaa ccatatgttt cctgccccaa acgcggtctt
     2461 taaacgattt tttatactct tccgcaccca ccaaccttcc gcgtaaggta ttcgccttat
     2521 tcaggctgcc tgaaacatcc aacactgcac gccggctgcc gcgatggtcg gcggtcagct
     2581 ctccggtatg tttgaaagaa gcggtaggtc gcttacggat caaattgacg gttcctcccg
     2641 gctctgaatt ggattgggtc aaccccgttg caccccgtac aacttcaata tggtcataaa
     2701 ccgccaaatc ggtactcgga gacacgtcga ttttcgccgt atatcccgaa cggcctgcaa
     2761 cattgacggt cataccgtct tcaccaatct gatcaatata gaaaccgcgt gacaaaaacc
     2821 gcgtctgcaa gcctgaatcg cgcacaacgt tgacacccgt cgtgtttttc attgcctctt
     2881 caagcgtatg caccgcctta tcgtcaaggc ggctgcgcgt gatgacgctg accgactgcg
     2941 gcgtatcctt gcccgcaatc ctcatacctg tggcggtgga catccgatct atcgtataag
     3001 aacgggtctt ttcggtcttg cccaacaaag catgagagcc gcgtacattg accgtatcca
     3061 gactgacggt attgccgtct gaaacaggca caacaccgtc tgcaaaagaa ccaccgtaag
     3121 ccgataacag cataacggtc agaattttaa gtgaaaaatg attttgattc atagagacct
     3181 ctgtaatatg caagtgtgca aatcgtccaa aggctctcac aactgttttg attttttata
     3241 ttaattgaaa aaaagtaatt ctcaattaaa tttatagata ggattgtatt cccattttga
     3301 caaaaaacaa actactcctt accgtttatt tcaaaaaacg ataacattgt attgaaaaat
     3361 atccgaattt aaatacagac cgccaatgca gaaaaaaaca cccaaattgg ctataatccc
     3421 gacaaacaca ctcaaggaca acaacatggc agcctcgccc gaagcaaaat tcaccgaaga
     3481 aaagattttg tgggtcaaac accacacgcc gaaactcatc actttcgcca tcagccgtcc
     3541 cgaatcctac cgctttaaag ccggacagtt ctcccgactc ggtttctacg aaggggaagg
     3601 ctttatttgg cgtgcctatt ccattgtttc cgcagaatat gccgacacgc tcgaatattt
     3661 tgccgtactc atccaagacg gccccatgtc ggcccgtttc gccaaaatgc aacagggcaa
     3721 caccatcctg ctcgataaaa atgccaccgg cttcctcctg cccgaacgct tccccgacgg
     3781 caaggatttg gtgatgctct gcaccggctc gggcatcgcc cccttccttt ccattctcga
     3841 acaacccgaa atccgtcaac gtttcgatac cgtcaacctg atacattccg tatcttttcc
     3901 cgaagaattg attttcaacg accgactcgc cgcattgact gaacatcccc tggtaggcga
     3961 atacggacac tctttccgtt tcgtccctgt taccacccgt gccgccaacc cctcgggctt
     4021 aagcggaaaa cgcattccgg aactcttaaa aaacagcagc atcgaacagg cgctgcatac
     4081 caagttcacc ccggaatcca cacggtttat gatttgcggc aacccggaaa tggtcaaaga
     4141 tactttccaa acgctgcttg acatgggtta cgccatgcac cgcaaccgca ttcccggtca
     4201 aatcatgatg gaaaacggct tctaaaaacc accctgcttg tccgatgcct tcggatggac
     4261 gggcaaaccg acacggcacg aaaaccgcgt cggcaaaaat gccgtctgaa aagttcagac
     4321 ggcatcttcg gacacattac ctgcaaacgg caacacattc caacccaaac cgattaggca
     4381 atcaatacga acggctgttt acatacttac cggctttcat caaccggtat cgatttaacc
     4441 gatttcctta atatttttcc tgtccgtttt aaacttcgcc ttaaacgcat ccggtaaatc
     4501 tttatcgaaa taccaaagcc cgtcacccat ttccaatgcg ccgcccattc catgcagaac
     4561 aactttttcc cccgttaaag gatcagtttc ggtaatttct acctcatgca tttttcccca
     4621 atccaaaata ggcaacttgt gtgcaaacgc caaaacctgt tcgtcagaac ggccgcacgc
     4681 cttatcgcat cggcctgaaa catatacggg acaaggctca tcctctgcat aaccgtccgg
     4741 caggataccg gctgcggcag gacacaatcc gtatgtttcc gcccacgacg acaaagcccg
     4801 tctcgccgcc tttttattgg caaataatcc ggtaggcgga ttatccgtca caccgttttt
     4861 taaatctgct gttttcgcat tcaatatgcc gtctgaacct ttttcaaacc tgacggtcgt
     4921 aaatgtttta agcagatttt tggcagatac gtaacaatcc accccataat gcctgaccaa
     4981 ttccgcttta accttatggg catgcagcct gcccaatgcg ggaaaaaaac ggacttcctc
     5041 cgtattgcac caatcaaacg gggcttttcc ggagtccaat aaagccgaaa cctcgctata
     5101 tacccgttca aacgtaccga tgtaatttac tttccctccg ccgtcgaaac aggccagcac
     5161 ccccataccg tcaggcaaac cgtacaactg ttccctcaac cgttcgggca gcgcggcagg
     5221 caacggtttc ggattcatca aacggaaaca ctgcctgatc cactcctcga ccccgtgttc
     5281 cgacaggctg tattccaaat aatcacacaa cgccgacaca tccgccatcg cacgatgcct
     5341 gtcttccaca acaatcccca acctttcgat gatactgtcc aggctgtgct tgtaaaattg
     5401 cggatacaga caccgggaca gctgcacact gcacaaagca ggcgatgaaa atccgatacc
     5461 cgcacgatga aactcatgct ttaaaaacgt atagtcgaaa cggctgttat gtgcaaccag
     5521 cacacaaccc ttcaataccg aaaacaactc gccggcaatc tctgcaaaaa caggcgcatc
     5581 ggcaaccatg ccgtctgaaa tccccgtcag ccccgccaca aactgcggaa tcggtttttg
     5641 aggattaacc aaccactcat gcctcaccac ccttccctgc tcaaacttga ccaaagccac
     5701 ttcggttacc ctgtcttcat acagattgcc gcccgtcgat tccaaatcga ccacggcaac
     5761 aggcattcca aaccgtaaaa ataccttttc cagcaagggc cagcgggaag caacaatcat
     5821 tttattctct ttaaattcaa acaacaaacc gatattttac acttttaagg catttcatcc
     5881 aacaaaacaa ttgacagaat ccgatgatta ccctaaaatt caaatctttc ttgcagtgca
     5941 cccgtagctc agttggatag agtatctggc tacgaaccag agggtcgggc gttcgaatcg
     6001 ctccgggtgc gccagtaaga aaatacaata tgcgcccatc gtctagcggt taggacatcg
     6061 ccctttcacg gcggtaaccg gggttcgatt ccccgtgggc gtgccaaatt ccaaatcccc
     6121 gagattatcc ctcggggatt ttttattgcc tcggcaaccc gttaccatat ctttacctac
     6181 ccccttcatc agaatctcag acgtaatcga atcatattca aacctttgcc gtgcaaaccg
     6241 atatcccata accggatgcg gtgtccgtcc aacattttac ccgattgaaa cgcctgatat
     6301 attgcacccc atcaacgtgg cattactttt cttaacaatc ccctttgaca gcaactgact
     6361 agggcttttt tatgccatca tcaaatttat agtggattaa ctttaaacca gtacggcgtt
     6421 gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     6481 ttaattcact ataatatttt ctctcccgat tgaaacaggc gtaacagaat atgcccgaag
     6541 ctccggctgc tttcttgttt accgccgcga tatttagagt ataataccaa atttgagcaa
     6601 tagttctaaa acagttagaa ccatttttca tgagcctgac tgattcgtac actcggagaa
     6661 actgatgcag aatatttttg accctttggt tattcgtgga aaatccctta cccccatcgt
     6721 gcaaggcggt atgggggtcg gtgtttccgc atcgggttta tccagcgcgg tggcacgtga
     6781 aaacggtatc ggaacgattg ccagtgtgga tttgcgccac cttcacgaag acctactcgc
     6841 cgaatcacaa atcaatccga gtgaagagaa atatacatct ttgaactgta ccgcattaga
     6901 cagggaaatc caaaaagcca aaagcgcttc agagggaaaa ggactgattg cggtcaacgt
     6961 gatgaaggcg gtcaaagacc acgccgcgta tgtccgccag gcttgcgaat caggggcgga
     7021 tgcggttgta atgggtgccg gcctgccttt agacctgccg gaaatgaccg agggctatca
     7081 taaagatgtc gcgctgctgc cgattctgtc cgaatcgcgc ggtattaata tcgtcttgaa
     7141 acgttggatg aaaaaaggca tattgcccga tgcgattgta gtcgaacatc ctgcccacgc
     7201 ggccggacat ttgggtgcat caaccgttga aggcgtaaac gatgccaagt tcgacttcaa
     7261 acgcgtgatt gaggaaacgt ttgaagtttt caaaagttta gggctggaaa gcgaaaaaat
     7321 cccgcttatt cttgcgggag gcatggcaaa ttttgaaaaa gtcaaaaccg ccctaaagaa
     7381 ctggggagca tccgccgttc aaatcggtac ggcttttgcc gttaccgaag aaggagatgc
     7441 acaccttaac ttcaaaaaaa cgctcgccgg tgcggaaact gaaaaagtag tcgaatttat
     7501 gtctgttgcc ggtttgccgg cgcgcggtgt ccgcaccaaa ttcctagaca gctacatcaa
     7561 gcgtgaaagc aaacttcaga caaacgccaa agccgacccg cgccgctgta cccaaggttt
     7621 aaactgccta accagttgcg gtctgcgcga cgggctttcc aaagccggac aattctgcat
     7681 tgatatccag cttgccgccg cattccgtgg agaagtagat aaaggcctgt tcttcagagg
     7741 taaagacccg ctgcccttcg gcaatgccat ccgcaccgtc cgcgagacga tacaatatct
     7801 gctgacgggg agcgaacctg ttgcaacgct cggacgctga catcaggtag gatttgattt
     7861 gcaaacaaaa tgccgtctga agcctttcag acggcatttt tcaggctgcg tttcggaata
     7921 gtgtcgaaaa aacggagaga aataaaaacg tttttatgta aacaaaacgc cttccgcatt
     7981 gtgtcgggca tacaacacaa atgccgtctg aagcccttca gacggcattt ttcaggctgc
     8041 gttctggaat agtgttaaaa aataaacggg atgagataca tttattttcg tccgacaaat
     8101 cagaccatca cgccgaatga tcaaataatg cctgcacggc attacatctg gcaaagcaat
     8161 gcaatgaaaa cacggctttt ttatttgctt tcagtattat tgaaaagttt gtccatcggg
     8221 gtcaaatcga ccgcattgcc ttggctggta atccatttgt tctcgacgcg ccacacgcct
     8281 gccgcgtcct ccacgcgcac ataccagtgg ttggtcggcg aaagggtttt gaacaccgcc
     8341 tcatattccg ccctgccgtt ctgcgcgctg ccgacgggct tgagggcgac ggtttgatcg
     8401 tccgccttgc gggtcgggtg catcagcagc aggttcaaag gctgtttgcc gtcaaactcg
     8461 ccgccgacaa acacttttgc cgcattcata tcgggggaaa tgagaacctg caccccgata
     8521 tgccgtctga cggcttcttc atcccgatga agctggatgt cgatatgctt gccgtcttta
     8581 taataatcgt ccgtaaccaa atctgtcgcg tgctgctgcg cgacaaaaaa catagcgacg
     8641 ctggcgatga cgacaaaaat cggccccgcc atcaagatcc acggccagac gtgtttgtac
     8701 caaggtttga ttggagtgtt ttgagacacg gttattctcc gataaaggtt gcatcttctt
     8761 ccaagacgac cggcttgccg tcgggcgcgc cgctttcgcg gtattggaag gtaaattcga
     8821 tagggtggct gcctttgtcc gcgtattccg gaatggtgga cacttggacg ggaagggtta
     8881 ccgtttcgcg cggggcaacc ttgataccgc cttcgggcag cccggtcagg gcgatttcgt
     8941 caaagccttt gacacttgcg gtaatgagct gttctttttc acttttgttg atgatacgca
     9001 ggctgtatgc gttttccagc cagcctttgg cgttttcgcg caccagtacg ccacggtctt
     9061 tcaaaatatc gacctcgacc attttgcgcg tggacaaacc ggccaggaag gcaatgataa
     9121 ctaacgccaa caccgcgccg taacctgcca cgcgcggtct gagcagccgt tttttaatgt
     9181 ctttttcaga atattcgtgt tccagcgcgc tttcggtcgt ataacggatt aatccgcgcg
     9241 gatagcccat tttgtccata atctcatcgc acgcgtcgat acaggcggcg cagccgatac
     9301 attggtattg cagaccgttg cggatgtcga tgccgacggg gcagacttgg acgcacatcg
     9361 cacagttgat gcagtcgccc aaacccgcct cttccttatt gaccgttttc ttgcgcgcgc
     9421 cgcgcggttc gccgcgttcc gcgtcataag aaacaatcag cgtgtccttg tcgaacatcg
     9481 cgctttggaa acgtgcatac ggacacatat gcaggcatac tttttcgcgc ataatgtggg
     9541 cgaagaagaa ggtcataaag ccgtaaaacg ctgtggcaaa catcgcgccg ccgcctgctg
     9601 ctccagtgaa taaatcggga acgaactggc ggatagggac aaaccagcct gcaaacgtga
     9661 tgcccgtcca cgcgcagaca aggaaaatca gcaggtattt ggtggctttg atgcggattt
     9721 tagtgaaatt ccacggcgat ttttccagtt tcagccgttt gtttctatcg ccttcgacca
     9781 ggttgtcgat ccacagcata atttcggtgt aaaccgtttg cgggcaggaa tagccgcacc
     9841 acagtcgccc ggcaatcgtc gtccaccaaa acagcccgaa ggcgcaaatc atcagcagca
     9901 aggcaaggta aatcaaatcg cccaccccca acgacaatcc gaaaatgaag aaatgccgtt
     9961 cggggatatt gaaaacgacg gcctgcctgc cgctccagtt gaaccacgga atcacataaa
    10021 acacaaactg cgtcgccaat acggcggcga tacgcagttt ggcgaaccgt ccttccgctt
    10081 ttttcggatg gatgcgttcg ccttcgggat ggatttgaat cacgctggca cgcggatcaa
    10141 acgttttttt cgctttcagc gcggcttttg tttgttcgga cgtgccgatt ccggatgccg
    10201 gactgccggc ttggttttcc gtggtcattc tgcattcctt agatttttga ttgatggttt
    10261 gcccgttacc gccgccgttt gcttttcaga cgtcattttt cttgtttttt aaggcgttgt
    10321 gtttcaagtt ttgagaaaat ccgtttttcc caaaatatat ttccgctatt gtacaacttt
    10381 atgcgccgtc cggatgtatg gggcggatac atttcccatc cgcatcaaaa cgcctggatt
    10441 ttaccttacc gcccgaacaa aatccgaata cggttaaaaa aaaagactaa aaaaaccgac
    10501 acccccatat cggcagaacc gacggcgcaa gctcataaac aaacgctatc gacaatccgg
    10561 cacacaatct ataacttttt atttcaaaag gaataatggc aggcttcgcc tgcaaatcga
    10621 aaatccttcc ccaccggtct cctgccgccg cattcccccg cgtccgccct tttcttgaaa
    10681 gcataagcga atcgggcgat aatcaacgct ttccgattat ccacttatcc aaaacaccag
    10741 caaggaaaat acaaaatgtc tcaactggca aacgcaatcc gcttcctctc ggccgatgcc
    10801 gttcaaaaag ccaattccgg ccaccccggc gcgcctatgg gtatggcgga aatggcggaa
    10861 acattgtgga cgaaattcct caatcacaac cccgccaacc ccaaattcta caaccgcgac
    10921 cgcttcgtcc tctccaacgg ccacgcgtct atgctgttgt acagcctgct gcacctgacc
    10981 ggctacaatc tgagcattga agacttgaaa aacttccgcc aactgcacag caaaaccccc
    11041 ggccatcccg aatacggcta taccgacggc gtggaaacca cgaccggccc gttggggcaa
    11101 gggattgcca acgcggtggg catggcatta gccgaaaaaa tccttgccgc cgaatttaat
    11161 aaagatggtt tgaacatcgt cgatcattac acctacgtct tcatgggcga cggctgcctg
    11221 atggaaggcg tatcgcacga agcctgctca ctcgccggca ctttgggctt gggcaaactg
    11281 attgttttat atgatgacaa caatatttcc attgatggta aagtggacgg ctggtttacc
    11341 gaaaacatcc cgcaacgctt tgaaagctac ggctggcacg tcgttcctaa cgtaaacggt
    11401 catgacaccg ccgccattca agccgccatc gaagccgcac gtgccgaaac cggcaaaccg
    11461 tccatcatct gctgtaaaac cttaatcggc aaaggcagtg ccaataaaga aggcagccac
    11521 aaaacccacg gcgcaccttt gggcgcggac gaaatcgaag ccacgcgcaa acatttgggc
    11581 tggacttacc ccgcctttga aatcccgcaa gaaatttacg atgcgtggaa tgccaaagaa
    11641 caaggcgcga aactggaagc cgactggaac gaactgttcg cgcaatatca agccaaatat
    11701 cctgccgaag ccgcagaatt tgtgcgccgt atggataaaa agctgccgga caatttcgat
    11761 gaatacgttc agacggcatt gaaagaagtg tgcgccaaag ccgaaaccat cgctacccgc
    11821 aaagccagcc aaaacagcat cgaaatcttg gcaaaagagt tgcctgaatt ggtgggcggt
    11881 tcggccgacc tgaccccgtc caacctgacc gactggtcaa acagcgtctc cgttacccgc
    11941 gacaaaggcg gcaactacat ccactacggc gtgcgcgagt tcggtatggg tgcgattatg
    12001 aacggtcttg ccctgcacgg cggcatcaaa ccattcggtg cgaccttcct gatgttcagc
    12061 gaatacgagc gcaacgccct gcgtatggcg gcactgatga aaatcaaccc tgtatttgtg
    12121 ttcacccacg attccatcgg tttgggcgaa gacggtccga cccaccaacc catcgagcaa
    12181 accgccaccc tgcgcctgat tccgaatatg gacgtatggc gtccgtgcga caccgccgaa
    12241 tctctggtgg cttgggcaga agccgtcaaa gccgccgatc atccgtcctg cctgattttc
    12301 agccgtcaaa acctgaaatt ccaagcgcgc agcgaacaac aactgaacga catcaaacgc
    12361 ggcggctacg tcatcagcga agcccaaggc aacgcccaag ccgtcatcat tgccaccggc
    12421 tcagaagtcg agctggcttt ggaagcgcaa aaagcactcg ccgcgcaaaa catcgccgta
    12481 cgcgtcgttt ccatgccgtc caccaacgta ttcgaccgcc aagacgccgc ctatcaagcc
    12541 gccgtcctgc ccgaaagcct gccgcgcatc gccgtagaag ccggccacgc cgacggctgg
    12601 tacaaatatg tcggactgaa cggcgcagtc gtcggcatca accgcttcgg cgaatccgcc
    12661 cctgccgatt tactcttcaa agcattcggc tttaccgtgg acaatgtggt tgatacggtg
    12721 aaatccgtgc tgtaacccca cacctaaaca aatgccgtct gaacgctttt cagacggcat
    12781 ttttttgccc tcccgccttg atgcttcctc attccgccaa tctccattat aatatttgcg
    12841 aatcactctt attcacattt caaaaggaga aacgcatgag cacccgtacc gaacatgaca
    12901 ccatgggcaa tgtcgaagtc ccgtccgaag cctattgggg cgcgcagacc cagcgcagcc
    12961 gcaacaattt caaaatcggc ggcgaaaccc tgccgcagcc gctgatttat gctttggcgc
    13021 tggtgaaaaa agccgctgcc gcgaccaatg tttccctcgg caggattaag ccggaacagg
    13081 cggatttgat tacgcaggcg gcggatgatg tgttgaacgg caggcttgac ggacagtttc
    13141 ctttagtggt ttggcagacc ggctccggca cgcagtccaa tatgaacatg aacgaagtgc
    13201 tggcgaaccg cgccaacgaa atcgccggta cgggtttggc ggcgtatcag cccgtccatc
    13261 ccaacgacca tgtgaaccac gcgcaatcga ccaacgacgc attcccgacc gccatccacg
    13321 ttgccgccgc gattgaaatc aaccgccacc tcatccccgc cgtaaaagcc ctgcgcgaca
    13381 ccttggacaa aaaagcccaa gccttcgccc ccattgttaa aatcggccgc acccacttgc
    13441 aggacgcgac gccgctgact ttgggacagg aattttccgg ctacgtttcc cagctcgacc
    13501 acgacttagg ccgtctgaac gatgcgctta aagacttgta tgaacttgct ttgggcggta
    13561 cggcggtcgg aacgggtttg aacagccatc cagaatacgc cgaaaaagcc gctgctaaac
    13621 tcgccgaatt gtccggcttg ccgtttgtca gtgcgccgaa caaatttgaa gccttaggcg
    13681 gacgcgatgc cgccgttgcc gcttcgggcg cattgaaaac gctggcggca agcctgaaca
    13741 aaatcgccaa cgacatccgt tggctggcaa gcggcccgcg ttgcggtttg ggcgaaatca
    13801 aaatccccga aaacgagccg ggttcgtcca ttatgccggg caaagtcaac ccgacccaat
    13861 gcgaagcgat gaccatggtg tgctgccaag tgttcggcaa cgacgttacc atcggtatgg
    13921 cgggcgcgtc gggcaatttc gagctgaacg tctatatgcc cgtcatcgcc tacaatctct
    13981 tgcaatccat ccacctgttg ggcgacgcgt gcaacagctt caacgaacac tgcgccatcg
    14041 gcatcgaacc cgtgccggaa aaaatcgact atttcctgca ccattccctg atgctggtta
    14101 ccgcattaaa ccgtaaaatc ggttacgaaa acgccgccaa agtcgccaaa accgcctaca
    14161 aaaacaacaa atcgttgcgc gaaaccgccg ttgagttggg cttgctgacg ggcgaagaat
    14221 ttgacgaact ggtcgttcct gccgatatgg ttcatccgcg ctaatccttc cctccaataa
    14281 aaatgccgtc tgaaacctcg ttcagacggc attttccgtt gcctgcgaac tagcggcgtt
    14341 tgaacagcct gtcccccacc gccgctgtaa ccgcaccccc gaccacgacc agtgcgccaa
    14401 cataacccaa accgttcata tccggcgcgg caaaagtatc aggcatcaca taatgcccga
    14461 gcaaagaaaa tattacggta aacacgggga gcaaggttgt taccgcgctg actttggaag
    14521 cctcccaatg tttcaacgcc tcgccgaacg agccgtaacc gattaacgta ttcaagcagc
    14581 aatacgcaaa acaaacccac gccaacgtac cgtccaaact tccgatgtgt gccggttcgg
    14641 caaacggcag gaacacggcg gcacttgccg cataaatcaa cagcagaatc tgttgcggcc
    14701 cgaattgcgc cgacagcagc ttttgcgcca cggcataaca cacccatgcc atactgcctg
    14761 ccgcacacag caacacgccc ttcgcatacg cgcccaaacc cgacaactcg ccgaatttat
    14821 cgttaaaaaa cataagcaaa ccggcaagca gcaaaaccaa gccgattttc tgagcggcag
    14881 tcatccggtc tttaaacacc aacacaccga caacaatcat cgtaaacggc gaaatctgcc
    14941 acaaaacctg cgtcgtggtc ggcgaaatat aatgcagccc ttgggcaatc agcacaaagt
    15001 ttgccgaaat gcccgccacg ccgagcagca gcagcctgaa tgagcaccaa gaaaaatccc
    15061 gccgcttcgg cagccgcccg cccagtgcca gcaaaacaaa caataccgcc gccgccacgg
    15121 taaaacgcac ccacaccagc gtcggcgcat cgacaaactt caatacctgc cgcacggcaa
    15181 tcggcagcgt tccccacgtc atcgccgcca aaagtgccaa cgcgaagcct aggagcggcc
    15241 tttggttttc catcctgatt ttcctatttt ttaaacaacc gtattgccgg acgatgccgg
    15301 tttgccgcat cgggcaatga tggttcaagc gtttggcgtt tgattccaac cctttgattt
    15361 caaacaaacc ggctgaagcc cggctattgc ttcgcgctat ttgaaaacac cgcctgaatt
    15421 tt
//
LOCUS       DAWFJW010000040        15225 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFJW010000040 DAWFJW010000000
VERSION     DAWFJW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.040"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..204
                     /locus_tag="WGR19_001013"
     CDS             <1..204
                     /locus_tag="WGR19_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798559.1"
                     /translation="KKYEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNAS
                     GGCIDGFGHHGLQLYNRALGYGN"
     gene            224..406
                     /locus_tag="WGR19_001014"
     CDS             224..406
                     /locus_tag="WGR19_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798560.1"
                     /translation="MPIPPAALMVEPVRPSPPKDGKTATLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(451..621)
                     /locus_tag="WGR19_001015"
     CDS             complement(451..621)
                     /locus_tag="WGR19_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0798561.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            634..852
                     /locus_tag="WGR19_001016"
     CDS             634..852
                     /locus_tag="WGR19_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798562.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            849..1175
                     /locus_tag="WGR19_001017"
     CDS             849..1175
                     /locus_tag="WGR19_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798563.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFCVEFSATAPADVIGILRKYYSVFSDGQKVEAASRHPECHAVRIV
                     FGDVPV"
     gene            1187..1453
                     /locus_tag="WGR19_001018"
     CDS             1187..1453
                     /locus_tag="WGR19_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798564.1"
                     /translation="MDFEFGFKTLWPIATAAFWFWVNGISSCLKEADKRIDDLKEELH
                     AVKLSYHTKQDAQDDRKNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            1450..1647
                     /locus_tag="WGR19_001019"
     CDS             1450..1647
                     /locus_tag="WGR19_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798565.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            1650..2156
                     /locus_tag="WGR19_001020"
     CDS             1650..2156
                     /locus_tag="WGR19_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0798566.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQATARAWKRSDK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(2189..2665)
                     /locus_tag="WGR19_001021"
     CDS             complement(2189..2665)
                     /locus_tag="WGR19_001021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798567.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELVPGSFVYRLKAEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDIEVFSG"
     gene            2677..4299
                     /gene="terL"
                     /locus_tag="WGR19_001022"
     CDS             2677..4299
                     /gene="terL"
                     /locus_tag="WGR19_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0798568.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHEFLFSRLPEIIHSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            4299..5867
                     /locus_tag="WGR19_001023"
     CDS             4299..5867
                     /locus_tag="WGR19_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0798569.1"
                     /translation="MAKKDNKTKIQKPEAALQTDAAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLMMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGMTSAGNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEIRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5854..7149
                     /locus_tag="WGR19_001024"
     CDS             5854..7149
                     /locus_tag="WGR19_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ0798570.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDSYPTTAYKAPDGSLYTADKGFGYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            7259..7675
                     /locus_tag="WGR19_001025"
     CDS             7259..7675
                     /locus_tag="WGR19_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0798571.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKLLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            7906..8967
                     /locus_tag="WGR19_001026"
     CDS             7906..8967
                     /locus_tag="WGR19_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798572.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            9017..9355
                     /locus_tag="WGR19_001027"
                     /pseudo
     CDS             9017..9355
                     /locus_tag="WGR19_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9429..10040
                     /locus_tag="WGR19_001028"
     CDS             9429..10040
                     /locus_tag="WGR19_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0798573.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            10037..10597
                     /locus_tag="WGR19_001029"
     CDS             10037..10597
                     /locus_tag="WGR19_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0798574.1"
                     /translation="MSYQDILRGLLPPVLYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            10608..12584
                     /locus_tag="WGR19_001030"
     CDS             10608..12584
                     /locus_tag="WGR19_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0798575.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFPSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLRADGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRRDGLLEALQD"
     gene            complement(13328..13630)
                     /locus_tag="WGR19_001031"
     CDS             complement(13328..13630)
                     /locus_tag="WGR19_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0798576.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(13602..13889)
                     /locus_tag="WGR19_001032"
     CDS             complement(13602..13889)
                     /locus_tag="WGR19_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0798577.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            13969..14574
                     /locus_tag="WGR19_001033"
     CDS             13969..14574
                     /locus_tag="WGR19_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798578.1"
                     /translation="MTIYFKNGFYDDTLGGISEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAATRGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            14553..14849
                     /locus_tag="WGR19_001034"
     CDS             14553..14849
                     /locus_tag="WGR19_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0798579.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKILYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            14846..15053
                     /locus_tag="WGR19_001035"
                     /pseudo
     CDS             14846..15053
                     /locus_tag="WGR19_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3902 a         4053 c         4180 g         3090 t
ORIGIN      
        1 aaaaaatatg aagtcaaggc gcacgctgtc ggcatggctt tggcgaaaaa acaggcggaa
       61 gtcagccgtc tgaaaacgga aaataaaaag gaaatcgaaa atgtccttac tcaagaccgt
      121 aaaaatgcca gcggcggttg tattgacggc tttggccatc acggcctgca gctctacaac
      181 cgcgccctcg gctacggaaa ttaaggttgt tgaaaaggcg gtcatgccga taccgcctgc
      241 cgcgttgatg gtcgagccgg tacgcccgag tccgccgaag gacggcaaga cggccacgct
      301 gttggaacac gccgctgagt ttggcggcta tgttgccgaa ctggaaaacc aaaatcaggc
      361 ttggcgcgac tgggcgggca atcactcccg caaagtcgga aactgacaaa aaagcccgcg
      421 tagggcgcgg gctgagggtg aaagcggatt ttatacctct tttacagggg tagcggcggt
      481 agtgcttttc agcaaatcga ctgcgtgctg acagttttgc ttgctggtgt agccttcgcc
      541 ctgagcgatg atttcatggt tggctgcttt caaacgccaa cggtattcgc cttttgcgtc
      601 tttatagatt tcaaaataca taaggtttct cctatgaatg agtacacgtt ttcttaccgc
      661 tttaacggca agtcctggtc attgagcatt tgggcggaca accctgaaga agccagggcg
      721 aaatttcggg ctgcacgaga aaatgcgcac tatgacggcg aagttgtagc aaaggtttat
      781 acatttgtaa atatttcgtg ggttaagaaa ttgtacaagc ggacaaaata tttaatgggt
      841 atcaaagaat gacctaccgt gaattagttg aacgtcagtt ggctgtgcgc catgccgatt
      901 tggaattggg cttaagccgc gcacgcgagc aagagccgtt tgtcattcat gtttccgatc
      961 tgttggataa ggcaggcatt gagtacgcgg tacgcatgga taaggatttt cagacgacct
     1021 tttgtgtgga gttttccgcg accgcccctg ctgatgtgat tggtattttg cggaaatatt
     1081 actcagtctt ttcagacggc caaaaggtcg aggcggcgag tcgtcatccc gaatgccacg
     1141 cggtccgtat cgtatttggc gatgtgccgg tttaaagggg ttttaaatgg actttgaatt
     1201 tggttttaaa accctgtggc cgattgcgac ggcggcattt tggttttggg tcaacggcat
     1261 ttcaagctgt ctgaaagagg cggacaagcg tatcgacgac ctgaaagagg agctgcacgc
     1321 ggtcaagctc tcgtaccaca ccaagcagga tgcccaagac gaccgcaaaa atatcgcagc
     1381 gtctttggga cgcatcgaaa acaagttgga aaaagtaaac gaaaaactgg acaggaaagc
     1441 ggacaaatca tgagcgaccc gattttggaa gccttggcgc gtattgaaaa caagactgat
     1501 caaacgctga aaaatcagaa ggaaatgcag gcggaaattg cgcaaatccg ccaagacacg
     1561 aaacgcacgg ccattacatt cggcgcactg ggcggcggcg tgattacggt cggctgggaa
     1621 ttgcttaaag cgaaaatggg actgtaatta tggctcaccc gcaagaaatc cgtgaaaagt
     1681 tacgccggct ctatgtgagc ggcgagcaaa ctttggaaac ggcggccttg atgtgcgaaa
     1741 tcccgcaggc cactgcgcgt gcgtggaaac gttcggataa ggaaaaaggc gacgactggg
     1801 ataagatgcg cgccgcttac actttggccg gcggcggtat tgaggacttg agccgtgcga
     1861 tgttggccgg ttttatggtg cagtacaaca gcacgatgac gatgctgcag gattcgagta
     1921 ccgaagattt gccgccgtcc gaccgtgcga agctgttggc cagcctggcc gatgcgttta
     1981 cgaaaaccgt atccgccaat gcccgtgtga tgccggaaac gtcaaaactg gcgacggctt
     2041 tggaattgat tgagttcttg atggcgtttg tgcaagaaaa acatcccaaa catttgcctg
     2101 cctttgtgga ggtattggag ccgtttgggg tggaagtgga gaagaagttt ggttaatagc
     2161 cgtctatcaa tctcccataa cgtgatcctc atcctgaaaa tacttcaata tctgacttta
     2221 aacgctctcc caatattatg gtttggcaac gggaacagga atatttatga aaagcacctt
     2281 catacccatt gaattgaaga atcgatttga caccctgctc gtaacattgg ggacaaatat
     2341 catgaattgg ctcgctgttc gccatctccg ctttcaatcg atagacgaaa gaaccaggaa
     2401 caagttcata caattggtag cgttgcttct ctgtagacca attttttact gccaccagtt
     2461 ctttttccaa ctcatctata cggctaattg ccgccgtata agaagattgg gcttcaaata
     2521 aatcagtttg aataccagca atgatttgat taagttcgat ggttttttgc gctacctctg
     2581 cctctgtttt gagctggtta aaccctttta aaagtgatcc tgcttctttt aggcctataa
     2641 atgcgttaga aatgaatcca gtcattataa atactcatga aaacaaaaga attcctcaaa
     2701 tcccttgccg aactggccgc cagcctgcgc caagtcatcg aagcggaagt ggacggcttc
     2761 gatgcgtcgc ccaaggctat tgctgcacgc cgtgccaagg tgtttgaccc ggtaggcggt
     2821 tacgagtatt tcgtcaacac ctactttccg cattatatcc gctcccctga aaaatccgaa
     2881 ctgcatgagt tcttattcag ccgtctgccg gagattatcc actcccccaa aggggaaaat
     2941 gaggcggtag gtgcgccgcg tggcgagggt aagtcgacgc aggttactca gttgtttacg
     3001 ctgtggtgta ttgtgacagg ccaaaaacat tatgccgtta ttgtgatgga cagtattgac
     3061 caggcatacc cgatgctcga agccatcaag gcggaattgg aatttaatcc gcgcttgaaa
     3121 accgactttc cggaagtatg cgggcagggc cgtgtatggc aggccggtac gattgtgacg
     3181 gccaatgacg ttaaagtcca agtggccggt agcggtaaaa agctgcgcgg tttgcgtcac
     3241 ggcccttacc gtcctgactt aactgttttg gacgatattg agaatgacga gcaagtccgc
     3301 aacccggaac agcgcgacaa gctcaatgcg tggctaacta agactgtatt gcctttgggc
     3361 ggggtcggcc agaaatacga tgtgatatat atcggcacga ttttgcatta cgacagcgta
     3421 cttaaccgca ctttgaataa cccgttttgg cacggtatta agtttaaggc gatgaaacgc
     3481 tggcccgacc gcatggattt gtgggacaga tgggaggaac ttttccgcaa cgacggcgag
     3541 acggtggccg aggcgtttta tctcgccaac aaagacgaga tggagcgcgg cgcagtcact
     3601 tcttgggcgg cgcgtggcgt actcgcgctg atgaaaatcc gcgcccgcga cggtcatgcg
     3661 acgtttgaca gcgaatatca aaacgacccg gtcagcggcg aagatgcgcc gtttgccaag
     3721 tcgatgaagt tttggaacga cctgccgtcc gatttggtgt atttcggtgc gctcgacccg
     3781 tcgctgggta aagcgggggc gagccgtgac ccgtcggcga ttatcattgg cggttatcaa
     3841 cgtgaaaccg gcaaactgta tgtcgtggaa gctcagatta aaaaacgtct ccctgatttg
     3901 attattgagg acgttatcag attgcaccgt caatatcgtt gcaaactgtg gtttgttgag
     3961 acggtgcagt ttcaggagtt tttaaaagac gagctggtca agcgcagtgc ggcgcgtgga
     4021 atacctgtcc cggcgcgggc ggtcaagccg gtatcggaca agctcttgcg gattgagact
     4081 ttgcagcctc acatggcgaa cggtttgatt ctgttgaatg agagccaaca aacgctgata
     4141 cagcagttcc gccattttcc aaaggctgat catgatgatg gtcctgatgc cgtgcatatg
     4201 ctctggtcgg gggcggtggc gaattgtgtg ccgatagaat ggcaaagccc taccgataac
     4261 gattttgatg acgagataaa aagtaaatgg agccgataat ggcaaaaaag gacaataaaa
     4321 ctaaaatcca aaagcccgag gctgcattgc agacggatgc ggctcaaatt acggcgaccg
     4381 gtcgggttat cgccgagcat ccgtccaatt ttattacgcc gcaaaagatg cgggccctct
     4441 tcgaggacgc agaaagcggc gacatccgcg cccaacacga gcttttcgcg gacattgagg
     4501 agcgcgacag cgacatcgcg gcaaatatgg gtacgcgcaa acgcgcgctg ctgacgctca
     4561 actggcgcgt cgccccgccg cgaaatgcga cgcccgaaga agaaaagctg tccgaccaag
     4621 cctacgaaat gatggacagc ctgcctaccc ttgaagacct gatgatggat ttgatggacg
     4681 ctgttgggca cggattttct gcgttggagg tcgagtgggt attttcagac ggcctttacc
     4741 taccccgaaa ctttatccac cgcccgcaaa gctggttcaa atgggacaaa gacaacgggc
     4801 tgctgctgcg tacccgcgaa aatccggaag gcgaagcgtt gtggccgctg ggctgggtcg
     4861 ttcataccca aaaatcgcgc agcgtccagc aggcgcgcaa cgggcttttc cgcacgcttt
     4921 cctggctgta tatgttcaaa cactacgccg tccacgattt tgccgagttt ttggagctgt
     4981 acggcatgcc catccgtatc ggcaaatacg gcgcgggcgc aaccaaagag gaaaaaaaca
     5041 ccctgcttcg agcggtggcg gaaatcggtc acaacgcggc aggcatcatg ccagaaggta
     5101 tggaaatcga gctgcacaac gcggcaaacg gcatgacttc cgccggcaat ccgtttttgc
     5161 agatggccga ctggtgcgaa aaatcggcgg cgcggctgat tttggggcaa acgctaacca
     5221 gcggtgcgga cggaaaatcc agcaccaacg cgctgggcaa tatccacaac gagatacgcc
     5281 gcgatttgct ggtgtcggac gcaaaacagg tggcgcaaac catcacaagc caaatcatcg
     5341 gaccgttcct gcaaatcaac tatccccatg ccgacccaaa ccgcgtgccg aaatttgaat
     5401 ttgacacgcg cgagccgaaa gacatcgcgg tctttgccga cgctatcccg aaactggtgg
     5461 atgtcggcgt acaaatcccc gaaagctggg tgcgcgacaa actggtcatt ccagatgtgc
     5521 aggagggtga ggctgtgttg gtgcggcagg taccggacaa tccggtaaac agaactgcat
     5581 tggcggcttt atctgcccac accgtaccat ctaaggctac gggcaggcat caggaaatat
     5641 tggacggcgc gttggatgac gcgctggttg agcccgattt caattctcag ctcaacccga
     5701 tggtgcgtca ggcggttgcc gcacttaatg cttgcaacag ctacgaggag gcagatgccg
     5761 cactgaatgc gctttatccg aatttggaca acgcgaaact gcgtacctat atgcagcagg
     5821 ccttgtttat cagcgatatt ttgggacaag accatgcccg cgcctgattt gggatttgcc
     5881 ttaagtctgc cgccaaaaaa ggcaatcgag tggctggaaa gtaaaaaggt tacggcggag
     5941 agctaccgca atctgacagc ctccgaaatt gccaaagtct atacgattgc ccgcatgacc
     6001 gacttggata tgctcaacga catcaaaact tcgatggttg aatcggcaaa aagtggacag
     6061 tcgtttgacg attggcgaaa aggtatcttg aatctgctca gcaacaaggg ctggctgcat
     6121 ccgaacgggc ataacggtaa ggatatcatc gacccagcca ccggcgaggt attcggttcg
     6181 ccgcggaggt tggagacgat ttaccgtacc aacatgcaaa ctgcctacaa cgccggtcaa
     6241 tatcaaggat atatggcaaa tattgatgca cgaccttatt ggatgtatga cgcggtaggc
     6301 gacagccgca cccgtccggc gcattcggca atagacgggc tggtgtaccg ctacgacgac
     6361 ccgttttggg caacgtttta cccgcccaac ggctacaact gccgctgctc ggtcatcgcg
     6421 ctgtcggagc gggatgtgga acgccagggg cggattgttg ggcaaagcac ggcggacaat
     6481 ctggtcgaga cccataaaat ctacaacaaa aaaggcgaca gctacccgac gactgcctac
     6541 aaagccccgg acggcagcct ctacacagct gacaaaggct tcggctacaa cgccgggcga
     6601 atgaactacc gccccgattt agacaagtac gaccgtgcgt tggcgcatca atttgccaaa
     6661 gcggaaatgg gtggtgcgga ttttaaaacc agctttaaac agcttgaaaa agagttttat
     6721 gaagtcaagc aacgtttgga tattgatggc aagcccgata aagagcagaa aatcaaaatc
     6781 cgaaatgcgc tatcaagaca gcttaaattt gctgcgggtg tattgagcaa ggaaatgcaa
     6841 gaactggcag gtatgactcg agcgacggtg tggctgtctg atgatacatt ggtcaaacag
     6901 gtggacagcc gtgaggggca gaatttcgat gactcctact atgctttttt gccggatatg
     6961 ctgcaaaacc ctgaacatgt catccgcgac aatcgtgaat tgattttcac agctcgctat
     7021 aaaggctcgg cattgtgggc agttttaaaa tatattaagg aggtggatga gatttatcta
     7081 cagtcgtacc gaatcagtaa cgacaaagag attgccaaat ttatggcgaa gaagaaagta
     7141 ttgaaataga cgttgggcaa ggctcgaaat cacttgcaca cgctctcgga cgccctaacg
     7201 ggcaggctgc ggtatcgagg ttatcaccgc ttttccaacg tctgtacgga ataataccat
     7261 gattgatgtc aaaatagaca atatctttgt cgtcctaaac caaatcgagc ggcttggcaa
     7321 cgggatcgaa aaccgctacc tgctgatgcg ccgactgtcc gaaaccatgc acacggcggt
     7381 caagctcaat ttccgctacg caggccgtcc gaaatggttg gggctaaaat accgcgacgg
     7441 caagctgctt tcggattcgg gtcgtctgaa agacagtttt tccacactgt cagacaacga
     7501 taccgccctt gtcggtacga atatcgtcta tgccgccatc cacaacttcg gcggtatggc
     7561 ggggcgcaac cgcaaagttc ggattccgca acgggaattt ttgacgctga cggacgacga
     7621 caaacaggct ttgatggacg atgtgcagga ttatttttcg ggtctgatac cgtgaattta
     7681 taaaaccctc aaaaacgcgc tttttagcgc gtttttttat gcgggtaata caaacccctg
     7741 cccaagatat aaaaatcaat cctagactct tctaaaaagc ccctgaaaac gattaattgt
     7801 gtatcgcgcg gacaggtttt aaaaaaatgg cgggagggtt tgaagcacgc ctactctttg
     7861 ttgttttttc aaataggcaa aatgaccgta ttgagagagg tacacatgtc caaaaatgca
     7921 caaaaaaccc tacttgccgt gtgcagtttc gaggtgcagc caaaagacgg gcgaatccaa
     7981 ctgctgccat atggcgaatt tcgcgcagta gacggtcgtc cgactgatgt ccctgcgtgg
     8041 tatctgaccg aagaaaacgg tcatgatgtc gcgttgttgg ccaacagctc gcgcaatcag
     8101 ttggttgtcg attatgaaca ccagacgctc tacaaagaga aaaacggaca acctgcacct
     8161 gccgccggtt ggatgcgttg gctggagttc acgcctaaag gcatgtttgc cgaagtggag
     8221 tggacggaca aggcggctgc ggcaattgcc gcaaaagagt atcgctacat ctctgctgtg
     8281 ttttcctatg acacaaaggg atatgtaagc aaaatttttc acgccgcgct gacaaatttc
     8341 cccgcgttgg acggtatgga cgaggtgctg gcggcagcgt cggcgcaaat tttaaaaccg
     8401 gaaacggagc aaaaccctat gaaagagttg ttacagcaac tgttcgacct gcctgatgcg
     8461 ggcgaagaag aactgaaggc ggcattgtcc gcgctcgtgg aagccaagcc gaaagacgtg
     8521 gcattgtctg ccgacgtgtt cgcgcagctg gcggaaaaag acagccgcat cgcggcattg
     8581 acggcgcaaa ccgccaagcc tgatttgact aaatacgcgc ctatctcagt ggttcaagag
     8641 ctgcaaagca aagtcgccgc gctgactgcc aagcaggaag cagacaaagg caacgaattg
     8701 attaccgccg cgctgacttc aggcaaattg ctgcctgctc agaaggagtg ggcaaaaggc
     8761 gtattgaaac agccgggcgg cttggcattt ttgaccggct ttattgaaaa cgcccagccg
     8821 gtcgctgcac tggcaggctc gcaaacgggc ggcaaagcac ccgacgaacg cgtcgccgca
     8881 ctgactgcgg aagaggcagc cgcagcaaaa atgctgggca tgtccggcga agaatttgta
     8941 aaaatcaaag aaagcgaagg taagtaatgg acaaatcagc gattttgacc gcaatcacgg
     9001 cagcattccg caaagaattt caaacggggg cctgcaaaac cgcctgaacc ggttaaccgc
     9061 caaacaggtg cagccggtag cgcaggcggt gcgcctgtta tccacgtcat cgctgttgtc
     9121 ggtggcaacg gcattaatcg aggcgcatgg cgaagagatg actgcgcccg atttgattga
     9181 ggttaaccgc gccatgcgcc gccgtatgca ggccgagatt gccgccttgc gggcggtgca
     9241 gacggctgct gccgagtctg gtgggctgac gaccaacgcc gtgtataccg aggcttacca
     9301 aacggcagaa tccctgcgcg cggcggcagg ccgtctgaat gcgttggttg cggcggcacg
     9361 gcggaaatac cggcaatcgc cgacgagccg ggcgcggccg ccaatgtggc gacggcgagg
     9421 cgcaactgat ggccgccccc gccggtgtgg ccaccgaatg ccgccttacc gtacaaggcg
     9481 gcaccgaccg agaaagcgat gcctcactgc tggcgcgtct gttggaaatc atccgccgac
     9541 cgcccgcagg cggcaaccgt tacgactata aaaactgggc gttgagtgtt gacggcgtaa
     9601 ccagcgcata tgtttatccg ctgcgccgcg gcttgggtac ggtggatatt gccattacct
     9661 ccgccgacgg tgtgtcgtcg gaagaaactg tgcgccgcgt acaggcttat atcgacgaga
     9721 tgcgcccggt aacggcaaaa aatgcgctgg tactcaagcc aaccgtaacg gcggtgcctg
     9781 ttaccgtgca agtcaagctc gacggtatcg acttggacga ggccaagcgc cgcatacgga
     9841 cggccctaaa agaatatttc gacaccctga tccccggcga cggcctgact gtgtcgcaaa
     9901 tcgaggctgc tatcagcaat gtggatggtg tgatcgaccg ccgtctgact gcgccgacgg
     9961 ccaaccgtgc cgccgatacg gttaaccgca tcgagtggtt taaagcgggc gcgattaatg
    10021 taacggagat gccgtcatga gctatcaaga catcttgcgg ggcctgttgc cccccgtgtt
    10081 gtatgcccgc aatgccccgc gtgtgcgggc gcaggcagaa atagacggcg cagcgctgga
    10141 tgcggtggcg gaatcggctc aaagcgttgc cgatgccgtc gacccgcgca gcgccggcca
    10201 aatgctggcc gattgggagc gcgtattagg tttggacggt acgggcaaaa accgccagca
    10261 ccgtgtgttg gccgtcatgg ccaagctaaa cgaaacaggc ggcttgagta ttccttattt
    10321 tgtgcgtttg gccgaggcgg cgggctatca aatccaaatc gacgaaccgc agccgttccg
    10381 cgccggtgta aaccgcgccg gcgaccgtct tgcgccgcag gaaatcatgt gggtgtggca
    10441 cgttaacgtg cgcggcggca acaaccgcat tacccgattc cgcgccggta tctcggcggc
    10501 gggcgacagg ctgaccgatt acagcgatgc cgtgatcgag agcctgttca accgcctcaa
    10561 gcccgcccac accgctatcc gatttaccta ccgataagga ctaaaccatg caccccatcg
    10621 aaacccccga caagaccttc cacgacggcg acggcgtgtc cgaattgggc accatcctgc
    10681 ccgcgtggtg gctcaaccaa gtgcaatccg agctgctggc cgtgctgact gcggccggta
    10741 tccagccgga taagtcgcag cctaatcaat tactggcagc actgaataga ctggctgtag
    10801 ttaccaccgg caaccaagaa atcggcggcc aaaaaacctt tactgcccaa acccaattcc
    10861 caagcggcat ccatttatcc gccaaccaga cgaactggaa cggcggccac aaagcctaca
    10921 tcggcgcgga tgccgacaac gctcacatcg tcttcggcga cgacaccctc cgcctgcaca
    10981 gcgcaaacaa ccgcatttcc tacaacaacc acgacatctt ccacaaagcc aacaaaccgc
    11041 gttttgccga agacatcgaa ggcaaaccga acacactgtc cggatacggc atcggcaatt
    11101 tcaaagtcga aacattccgg ggcgatttga acaccctcaa aacagacggc atctattccc
    11161 tgccgacggc ggtcggcagc tccaacctgc ccgttgaaaa cactgcctgc catatccaag
    11221 tcatcgccgg tacgcaaccc ggctggtgca ggcagttggg ttatcccgcc tacacgtccg
    11281 acgtgtacga acgccaccaa acgagcagcg caaacgacaa ctggtccgca tggaaaaaac
    11341 tcaattcaga cggcatcccc gtcggcgcga tcgtatcctt tcccaaagcc gtccgaaacc
    11401 ccgcaggcta tttgcgggca gacggcacaa cctttgcgca aaacaccttc cccgaccttt
    11461 accgcgcctt gggcaacagc aaccgcctgc ccgatttaag ccgtaccgac atcggcatca
    11521 ccgcgtggtt tccgtccgac caaatcccga ccggctggct ggcgtttgac gacatccgca
    11581 cgcgcgtaac cgaaaccgct tatcccgagc tgtaccgtct gctgaccgga aaatacggca
    11641 gcatccaaaa cgtcccgcag gcggaagacc gctttatccg caacgcgggc aacagcttgg
    11701 cagtcggaac gaagcaggaa gacgaaatca aacggcacgt ccacaaagta ttttcacact
    11761 ggacaaacca cacagacgcg gcagccctcg gttacgaaga ccgcaacgaa aggcagagaa
    11821 gcgcgctcgt atcgacttgg acggacgaaa atttaaacga caacggcttt ttaaccccgc
    11881 gctcggacag caaaatggcg acaggcggcg acgaaaaccg ccccaaagcc ctggttttaa
    11941 aactgtgcat caaagccgcc gacaccttgg gcgaagccgt gttttggata aagtcccacg
    12001 gcgaaaccat caacgcaggc gcgctggacg cgggcacgct ggcgcaaaac ctccaagaca
    12061 aagccgaccg cgcccacacc cacaccgccg cccaaatcca agggctggac gaaaaaatca
    12121 gcaccgccgt tgccgcgcaa ttcacacgcc aaaccatcgg cggcgtggat attgtcagat
    12181 tccccgacgg cacaatgata cagaccggca gttacaggtt tacaagaagc ggcggcccca
    12241 tcgaaaacga agtcgtcttc cccgtcgcct ttgccgacgg caacgtcaaa tgcttcgtat
    12301 ccgaacgcca ttcgggacgc gttaccggcg atcgaaggca gcacaactgg ctgtttatcc
    12361 gcgcaaaaaa ccacgccgcc gccattatta ccaactggta cgaaggcagt tgcgactgga
    12421 tggccatcgg caaagccgcc tcgggaaacg ccgccagctc cccgataggc cccgaaatac
    12481 ctgaaaccaa cgaagaaccg caaagagaga gtggaagaac atcaaccgga ccccgaaacc
    12541 gccgccgccg ccgagacggc ttgctcgagg cactgcaaga ctagcgggct gtagagacgg
    12601 gctgtagaga tggctgtaga gatggctgta gagatggctg tagagatggg ctgtagagat
    12661 gggttgtaga gatgggttgt agagatgggt tgtagagatg ggttgtagag atgggctgta
    12721 gagatgggct gtagagatgg gcttcagccc gccgatccaa gcaatccgac cgaaaccggc
    12781 cgcgccgcca atcccccgaa acctatgccc cgccaatcct gccactcttc gtcattcccg
    12841 ccgctttcgt cattcccgcg aaagcgggaa tccagcccct aaacgcggca ggaatctatc
    12901 ggaaaaaaca gaaacccccg ccaccgtcat tcccgcgaaa gcgggaatct agaccccaaa
    12961 cgcggcagga atctatcgga aaaaacagaa acccccgccg ccgtcattcc cgcgaaagcg
    13021 ggaatccaga ccccgacgca acaggaatct atcggaaaaa acagaaaccc ccgccgccgt
    13081 cattcccgcg caggcgggaa tccagacccc aaacgcggca ggaatctatc ggaaacggct
    13141 gaaaccgaac ggactggatt cccgccttcg cgggaatgac ggaaagtggc gggaataacg
    13201 aaaggcggga atgaccgcgc aaaaagccgc tgcccccttc ggacggcacc ggcaacaaaa
    13261 aaccgcacgg ccgaaaccgc gcgggaaagg atagtcgggc gcgcccgata agcagcggcc
    13321 gcccccgtta tttcaattgg gcgacatatt ggcgcaaaac ctcgttgatg cgcgtctgcc
    13381 agcccttgcc gccggcgcgg aatttttcga ccacatcggc ggacaggcgt atggtaacga
    13441 gttgtttcgg ggttttgcct gtgtttcgtt tttgcattac gcccttttct tccaattgtt
    13501 tttgatggga aaaaagcacc tgtgccaagt cttcgggcag ggcttcggca atggggcggg
    13561 caagcgcaaa atcttcggcg gcaagttccc gcacttcgcc gtcagcgttt gttaaggatt
    13621 gacgttgcat attttttaac ctctctttta ttcgctttgc gaaaactgat gacacggatg
    13681 ccgtctttta tcggcgtaaa acagacaatg tgcaggcgtt gcgtatcgcc tagataagca
    13741 gcggcaacat aacgcggttc ggggtaatca aagcagacat cgggcacaat aacggccgtt
    13801 gtccagcgta tttgcccgac tgattcaaag ggcaaattcc gctcttcgat attgcgttga
    13861 tttttttcgg agtcaaattc aatcttcatt gcagcttgca gcgtattttg tcgttacatt
    13921 ataagcggca aaaaaccaaa atgtaaatac aaaaaaggaa accccgaaat gaccatctat
    13981 ttcaaaaacg gcttttacga cgacacattg ggcggcatct ccgaaggcgc ggttgccgtc
    14041 cgcgccgaag aatacgccgc ccttttggca ggacaggcgc agggagggca gattgccgca
    14101 gattccgacg gtcgccccgt tttaaccccg ccgcgcccgt ctgaatacca cgaatgggac
    14161 ggcaagaaat gggaaatcgg cgaagccgct gccgccgccc gtttcgccga acaaaaaacc
    14221 gccacggcat tccgcctcgc ggcaaaggcg gacgaactca aaaacagcct cttggcgggc
    14281 tatccccaag tagaaatcga cagcttttac aggcaggaaa aagaagccct cgcgcggcag
    14341 gcggacaaca acgccccgac cccgatgctg gcgcaaatcg ccgccacaag gggcgtggaa
    14401 ttggacgttt tgattgaaaa agttatcgaa aaatccgccc gcctggctgt tgccgccggc
    14461 gcgattatcg gaaagcgtca gcagctcgaa gacaaattga acaccatcga aaccgcgccc
    14521 ggattggacg cgctggaaaa ggaaatcgaa gaatggacgc taaacatcgg ctgaaaaaat
    14581 acgtttacca cctgttggta gccatcgacc aactgttcaa cgccctaacc ggcggcgcgg
    14641 cggacgaaac cctctcaagc cgcacctatc gcggcgcgcg gctcgcccaa aagcccaaaa
    14701 cccgctggaa gattttatat accctgatta acggcgtgtt tttcgaccgc caacactgtc
    14761 ggcaggcgta tatcagcgaa ctgaaaggca ggcagcacga cgcgcggttc aaccaaagcc
    14821 gcgccgccgg ggaaaagggg acgcgatgaa ctacttcggc atggtagagt ttctgcgcct
    14881 gatggcaatg gtgcggccgc catttgtctt cttctccggc acccgcagcg aactgcctgc
    14941 ttatcttgac ctcgtggcag aactgcgcga ccggatggga gcgttttgca ggcagccaaa
    15001 ccttgactgt gagcagcaca tcaacagcaa ttcaagctac gacgaccacc tgatttataa
    15061 gttctgaccg caagtagcgt actactttta aaggcataag ataatccccg tttaacagac
    15121 catataaacg gggataaatt tgtgcaaaag ctaatacaat ttcctacgct tcggcggtgc
    15181 aaaagctgcc gccaattcgt gcaaaagctg ccgccgcctt acagt
//
LOCUS       DAWFJW010000041        14974 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFJW010000041 DAWFJW010000000
VERSION     DAWFJW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14974
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.041"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(3..323)
                     /locus_tag="WGR19_001036"
     CDS             complement(3..323)
                     /locus_tag="WGR19_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HEZ0798580.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLPARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARSI"
     gene            519..977
                     /locus_tag="WGR19_001037"
                     /pseudo
     CDS             519..977
                     /locus_tag="WGR19_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            877..1245
                     /locus_tag="WGR19_001038"
     CDS             877..1245
                     /locus_tag="WGR19_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0798581.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1314..2852
                     /gene="lnt"
                     /locus_tag="WGR19_001039"
     CDS             1314..2852
                     /gene="lnt"
                     /locus_tag="WGR19_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0798582.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3116..3979
                     /gene="rpoH"
                     /locus_tag="WGR19_001040"
     CDS             3116..3979
                     /gene="rpoH"
                     /locus_tag="WGR19_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0798583.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4038..4184)
                     /locus_tag="WGR19_001041"
     CDS             complement(4038..4184)
                     /locus_tag="WGR19_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798584.1"
                     /translation="MLIHYINSANLSEKQLMRQCLVHVCFLISAIAMVWTDKIVYGTT
                     HKPH"
     gene            4463..5347
                     /locus_tag="WGR19_001042"
     CDS             4463..5347
                     /locus_tag="WGR19_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0798585.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGNQSLELNRDLVGQLAEINKDLR
                     KHYDLAKLGVADILRFPGVLASQRENADALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5431..5946)
                     /locus_tag="WGR19_001043"
     CDS             complement(5431..5946)
                     /locus_tag="WGR19_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0798586.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6021..6440)
                     /locus_tag="WGR19_001044"
     CDS             complement(6021..6440)
                     /locus_tag="WGR19_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798587.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6561..7559)
                     /locus_tag="WGR19_001045"
     CDS             complement(6561..7559)
                     /locus_tag="WGR19_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0798588.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIHEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGEKQTLAPIAVRRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            complement(7561..8040)
                     /locus_tag="WGR19_001046"
     CDS             complement(7561..8040)
                     /locus_tag="WGR19_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798589.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8092..8871)
                     /gene="pgeF"
                     /locus_tag="WGR19_001047"
     CDS             complement(8092..8871)
                     /gene="pgeF"
                     /locus_tag="WGR19_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0798590.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAESLGGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9057..9248
                     /locus_tag="WGR19_001048"
     CDS             9057..9248
                     /locus_tag="WGR19_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798591.1"
                     /translation="MFQQGMAHQAPHCVLPERDYPPIRFYRYKQMGFNRKGMGIKSIS
                     DTSRAMPSENQSPLSDGIV"
     gene            9325..10272
                     /locus_tag="WGR19_001049"
     CDS             9325..10272
                     /locus_tag="WGR19_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0798592.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(10424..11548)
                     /gene="rluD"
                     /locus_tag="WGR19_001050"
     CDS             complement(10424..11548)
                     /gene="rluD"
                     /locus_tag="WGR19_001050"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0798593.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11547..12350
                     /locus_tag="WGR19_001051"
     CDS             11547..12350
                     /locus_tag="WGR19_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0798594.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12408..14627)
                     /locus_tag="WGR19_001052"
     CDS             complement(12408..14627)
                     /locus_tag="WGR19_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0798595.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLVGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3577 a         4528 c         3687 g         3182 t
ORIGIN      
        1 cactatatcg accttgccgt cgggcaggat gtaccacgcc aaatccccgc ctgaagcacg
       61 gtgtcggact gcatttcgat tcggctatac gccttttcac gcaggctgta attgaatttc
      121 cacaaaccgc cgaacactgc cggcggacgc gcgaccagca tcaggaacgc cgcaatcgcc
      181 aaggcttccg ccgccccgtc caacacgccg gcgggagaaa ccagcagcaa aggcattccc
      241 aaacgtgcgg gtaaagtggt cgaaaccacg ctcagaaaca acagtgcgcc acccggcagg
      301 aacagactgc ccatccaatc cacgctaacg attctccctt aaaattcatt aaattaaaat
      361 cgaaagactg taacacggca ttttcttttc acgaaacttt acttcacgtc cgccacagcg
      421 ttcagacggc attttcctgt ttcatgccgc ctgaaaccgc ccaaatccgc gttctcctat
      481 ataatgtcgg actttgcgcc gcatttcagg attctttaat ggcatttgcc tcactattta
      541 ccctgccgga cgacatcacc gccgttttgg acgatgttgc cctgatgacc aaagccgccg
      601 caaagaaaac cgccggcgtg gtcggcgacg atttggcacc caatgccaac cgtgttacgg
      661 gcgtatcggc agaacgcgag ttgccgattg tttggtcgat ggcgaaaggt tctttttcca
      721 acaagccggt tttggtgcct gcggccctgc tgctttccgc cttcctgccg caactcatta
      781 cgccgctttt gacggcaggc ggcatttatc tgtgctttga aggcgtagaa aagctgctgc
      841 acaagtttct gcaccgacat gacgcgcatg ctcgtaatgg cggcaatcgg catcggcatg
      901 actgttttgg tgtacggaat cgtcgccgtc atcgtcaaac tcgacgactt aggcatgctg
      961 ctgatgcgcc gcccacaaac cttttcgcgc agtttggggc aaatgctgac tgcctttatg
     1021 ccttgcttta tgcgcggttt gagcgtggtc ggcacgcttg ccatgtttct tatcggcggc
     1081 gtactggtcg cccacaacct cggccttctg cacgatttcc tgcacgcgca acactgggac
     1141 gctggctggg cggagtactt cgccaacctc gttgtcgggc tgctttccgg ttcgattgcc
     1201 tgcgcccccg ccttgccgct gatgaatcgt ttcggcaggc attgatttct ttttcaaata
     1261 ccgatgccgt ttgaaagatg ttcagacggt atcttccgaa cagacagatg aatatggttt
     1321 ccaaactgga caaatactgg cagcaccccg ccctctactg gcctttgctc atcctttttg
     1381 ccgccgccac cccctttacc ttcgcaccct actaccactt ttggctgatg cccttgattt
     1441 tcggtgcctt cgtccgcctc atcgaactgc gtccgcgttt tgctgtctct tccgcctacc
     1501 tgttcggcct gaccgcatac acgacacagt tctactggat acacaccgcc ctgcacgacg
     1561 tttccggcct gcccgacctc tatgccgtac cgctgacctt cctactcccc gcctaccttg
     1621 ccctttatcc ggcactgtgt ttttggctgt ggaaaaaatt taccctgcct cggggcataa
     1681 aaatcggttt ggtactgccc atcctgtgga cactgaccga gtttgcccgc gaacgtttcc
     1741 tgaccggatt cggctggggc gcaatcggct actcccaaat caccccggac agcccgctcg
     1801 ccggctttgc cccattgggc ggcatccaca tggttacact ggcaaccgcc tttctcggtg
     1861 tctggctggt tttggcgagt gataacaccg cacgttcggg caaacgcctg cttccaatta
     1921 tcctgattgc cgccctgctt gccgcaggct acaccgcccg acaaaccgac ttcacccgcc
     1981 ccgacggcag ccgcagcacc gtcgccctgc ttcaaggcaa catcgaccaa accctcaaat
     2041 ggcgtgaaga ccaagtcatc ccgaccatac agaaatatta cgaacaggtc ggcaaaacca
     2101 ctgccgacat cgtcatcctg cccgaaaccg ccatccccgt tatgcgccaa aacctgccgg
     2161 aaaacatact ggcaaaattt gccgaacagg cgcaaaacaa cggcagcgcg ctcgccgtcg
     2221 gcatcagcca atacacttcg gacggcaacg gttacgaaaa cgccgtcatc aacctgaccg
     2281 gttatcagga aaacaatcag gacggcatcc cctactacgc caaaaaccac ctcgtcccct
     2341 tcggcgaata caaaccgctg cccttcctga ccacgccgct ttacaaaatg atgaatatgc
     2401 ccctttccga cttccgcaaa ggcggcggta agcaatccgc cctgctgatg aaaaaccaaa
     2461 aaatcgcctt caacatctgt tacgaagacg gattcggcga cgaactgatt gccgccgcca
     2521 aagatgccac cctgcttgcc aatgccagca atatggcgtg gtacggaaaa tccaacgcca
     2581 tgtaccagca cctccaacaa tcgcaggcac gggctatgga actcggacgc tatatggtcc
     2641 gtgccaccaa caccggcgca accgccatca tctcccccaa aggcaacatc atcgcccaag
     2701 cccaacccga tacggaaacc gtattggaag gacacatcaa aggctatgtc ggcgaaacac
     2761 cctatatgaa aaccggcagt tcatggtggt tgatgggcat attgacccta gccgcactga
     2821 ttcttttcat cttccgaaac aaagaacact gatacctgca cagcccgtcc ggacgggctg
     2881 tcttttttca aaccggctgt ttacatacaa ctacgtatgt aatataattc cgaaatcgga
     2941 ttgagccatc aggtttacaa aatcaatcaa acctgcctta caaaattttc ctgtctttca
     3001 aaccttagcg catatctcct gccaaacata caaaatacaa gccaagcagt gatatactgc
     3061 atcccaacac agcaaaccgc cgatacgcag tttcagccga aaggaaatgc cccaaatgaa
     3121 taacgctttc gcattacccg ccatccaaag cggcaacggc agcctcgaac aatacatcca
     3181 caccgtcaac agcatcccga tgctgtccca ggaagaagaa acccgcctcg ccgaacgccg
     3241 gataaagggc gacctcaacg ccgccaaaca actcatcctg tcccacctgc gcgtcgtcgt
     3301 ttccatcgcg cgcggctatg acggctacgg gctgaatcag gcagacctga ttcaggaagg
     3361 caatatcgga ctgatgaagg cggtcaaacg ctacgagcca ggtcgaggcg cgcgtctgtt
     3421 ttcatttgcc gtacactgga ttaaagccga aatccacgag tttattttga gaaactggcg
     3481 tttggttcgc gttgccacca ccaaaccgca acgcaagctg tttttcaatt tacgcagtat
     3541 gcgtaaaaac ctcaacgccc tgtctcccaa agaggcacaa gacatcgccg acgatttggg
     3601 tgtcaaactt tccgaagttc tggaaatgga acagcgcatg acgggacacg acatcgccat
     3661 catggcggac aacagcgatg acgaagacag cttcgccccc atcgactggc ttgccgacca
     3721 cgattccgaa ccgagccgac aactatcgaa acaggcgcat tacgccctgc aaacagaagg
     3781 tttacaaaac gctttggcgc aactggatga caggagccgc cgtattgtgg aaagccgctg
     3841 gcttcaagac gacggcgggc tgacgctgca ccagcttgct gcagaatacg gcgtctctgc
     3901 cgagcgcatc cgccagattg aagcaaaagc catgcagaaa ctgcgtggtt tcctgaccga
     3961 agaagccgaa gcggtttgaa atatacgcaa taaaataccc gacaaattgt tttgtcgggt
     4021 attttatttg gtaaacatca atgcggtttg tgcgtcgtgc cgtacacgat tttatccgtc
     4081 caaaccatcg caatcgccga aatcaaaaag cagacatgca ccagacactg ccgcatcagc
     4141 tgcttttccg acagattggc ggaattgata tagtggatta acaaaaatca ggacaaggcg
     4201 gcgaagccgc agacagtaca aatcgtacgg aaccgattca cttggtgctt cagcacctta
     4261 gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggttttgg ttaatccact
     4321 atacatttcc cttccttgca gcaacaaaag agcgtgattc caatctgccg ctgcaaaacc
     4381 ttcaattttt cacacccgaa aacgcttgcc gccatacaga tgtgatatat tgattccatt
     4441 ttaacgggct tcggagaacg acatgagcag cggaaatatc cacatccaca gcatgaccgg
     4501 ctttgccaac gcggcggcag agtgcggcag caaacgcatc aacctcgaca tccgcgccgt
     4561 caaccaccgc tttttggaca ttcaaatcag gatgccggat gatttgcgct atttggaaag
     4621 cggtatccgc gagaaaattt catcccatat cgcgcgcggc aaagtcgaat gcaaaatcca
     4681 aattcaagat gcggaaaacg gcaatcagtc tttggaactg aatcgcgatc tggtcggaca
     4741 gcttgccgaa atcaacaaag acctccgcaa gcattatgat ttggcaaaac tgggcgttgc
     4801 cgatattttg cgctttccgg gcgtattggc aagtcaaagg gaaaatgcgg acgcattggc
     4861 aaaggttatt gccgatttga ccgattcggc actgaaggac tttaccgccg cacgccggcg
     4921 cgaagggaaa aaattgggcg agcacctgtt acaacgcctc gaaagcatgg aagaaatcat
     4981 agatgcatta agcgaactct ttcctaccct gttggaaaca cataaagaaa aaatccgcgc
     5041 ccgccttgcc gaagcggtcg gcagcatcga caacgaccgc ctgcaacagg aatttgccct
     5101 ctttatccaa aaatccgaca tcgacgaaga gttcagccgc ctacgcaccc acattgccga
     5161 agtgcgccgt atcgttaccg aacacaaagg cagcagcggc aaacggctgg acttcctgat
     5221 gcaggaatta aaccgcgaag ccaacacttt gggcagcaaa tccatcgcta ccgaatgcac
     5281 ccaagcctcg gtcgagctga aagtcctgat cgaacagatg cgcgaacagg tgcagaatat
     5341 cgaataatcg gtttccccga taagcaaatg ccgtctgaaa gccctttcag acggcatttt
     5401 ttcagtttga aacctttgcg gtataaaggc ttaaggcttt cctttgaccg ccaccaatct
     5461 tgttcctgca agaaaatcat acagaaactg ccgatcggga ttcagcaggg cgaaccccca
     5521 cggtaaaatc agccaaatca atgccgcgcc ggccgcgccc ttgggcggaa tgccgaggaa
     5581 gtggcgcaat ccggcataag caagcatagg aatgaagacg atgaatatgc acgcccaaat
     5641 aaagcgcagg cggagtaagt gtagagacgg ctgtatgccg ttaaggtcgc acaaaccgat
     5701 tttccacgtc ctcatcgcca aggtctgtcc ctgcccgtgc cagttggcac ggaaataaag
     5761 ccaccatgat cccattatca ggatactcgt taccaatgca gaaaccgcaa tagaaacggg
     5821 attcagaaaa atggcggcaa taccggcaaa caatgctgcc aaacaggttg ccgcaccgac
     5881 cagcagcatt tcgtacagca tggcggcaaa gcggcgtttg agcggggcgg ttttcagtat
     5941 catcatcttt tcaatcggaa tatgccgtct gaaacgccaa caggcttcag acggcattca
     6001 tgcagatatg tctcatgtgg ttaacggtgc ggacgtttct gaccgcgcga ctgttttgcc
     6061 gccatttgat tgatttcctg acgtgtcggg cgttttttga acatagcgtg cgcctcgtcc
     6121 accgaacatt tttgcgacag gcagatcagg gtaatcgcat ctttctggtg ttgtccgccg
     6181 ctgtggtaca tacggcgcag gtttgcctcg ttgattctgc ctttttcggg atcgatgtcg
     6241 aattttttca ggcgttcgat aaactggcgt tgctgtcggc gggatgtcca aacaaaaccg
     6301 cccgcagcgg cggcgacgat gacggcggca agcaggatgg caagcggcca ggaatcgacg
     6361 atgagcacga taatcaggct gaggacggcg gtagtcagta aaatcaggcc gcccaagcgc
     6421 agtttggtct tattgtccat agggttgatg ttcctttatt gaggtggtcg tgccgtctga
     6481 aatgttttcg gacggattgg tgaatacttt tcaggattaa cggtatttta cagtattttc
     6541 ggattttggg gattaccgct ttattccgcc agcgacacga caagccgttt gaataccgtc
     6601 catgcgtcgc cctcttccgc gcctttgatg attcggtcga tttgggcaca ggttttgagc
     6661 gcgtcaagca ggcggacgac ggaaatccgt ctgaccgcaa tcggagcgag cgtctgcttt
     6721 tcaccccaaa gcctgaggct gttgcggacg gattggatgc tctgcccctg cttcagggcg
     6781 gcggcaagcc ggatcagcgt ccgcacgtct tcggcaaccg cccacagcaa cagcaccggt
     6841 tcttcgcctt cctcctgcaa tccgtccaac aatctgcata cgcgcgggac atcgcccttc
     6901 atccacgcgc ctgccagttg gaacgcgtca aagcgggcga cgttggcgac ggcggtttgc
     6961 gcctcatgga tattgacggt atgccctttc ggatacagca gcgcgagctt gtcgatttcc
     7021 tgacgcgccg ccaacaaatt gccttccacg cgctcggcaa acagtgccaa tgcgtctgcc
     7081 tcgataccca aaccgatttt gtccagccgt ccgcgtatcc attggggcaa ggccgccgcg
     7141 ccgaccggtt tggcttccca cacttccccc tttgccgcca atgcggcaaa ccatttggac
     7201 tggagctggg ttttctccag tttgggcagc aaaaccagcg ttaccgtatc ttccggcagg
     7261 cgggcggcaa aatcctgcaa cgcctcgccg ccggttttgc cgggttttcc gttggggata
     7321 tgcagttcca acagcttcaa atcggcaaac agacccgcac tgcctgcggt ttgcaacagt
     7381 tcgttccaat cgaaggcatt gtctgccgta taaacttccc gattaaggta accttgtttc
     7441 ttcgccgccg ccctcaatgc gtccaatgcc tcgatacgca acagttcttc ctcgccgtgg
     7501 atgacgtaca gaggtttcaa aggcgcgtcc gtatcaatgc gtccgatatg tgccgccacg
     7561 tcattccgcc ttcagaaagg tcaggcggcg gacaatctgt tcggcggcat cctgccgcat
     7621 ttccgcccac aatgccgctt cctcttcctg tttgcccaag atctcgttgt cggcataatc
     7681 caaaatgcgg cggacggaca cggtcatcgg tttgccgacc ggctcgccgc gtttcaatac
     7741 ctgcgcttca accgtcaata tcaaaagata ttcgttgatg actgccgcac gggtaacggt
     7801 gtaggtttcc ttgttttggg aaacgctgtc tatacgcagg gtcatctgcg cgccggcagc
     7861 atcgtccacc ctgcccgaag cctgatacag cgcggtttcc aaaggaaact gcaatgcctg
     7921 tccgccttcg atgtgccagc tccggtaggt cagcggcgga gaaatgccgt ctgcaccttt
     7981 caggtggaaa ccgcacgcgc ccagcatcag cactgcggca gtaagaaaca gtttgttcat
     8041 ggcttgcctt tctcaaaagc acaaagtcgg tagattatat cagcggcgtg ttcagacggc
     8101 attgccgtcc agccaaatca ggctcgccat acgccctgtc gctccgtcgc ggcggtagga
     8161 aaagaaagta tcccgttcca aaaccgtaca atgcgtgccg ccgtacacgc cgcccacgcc
     8221 ttcgcgcttc agaatcaggc gggcgagcgc gtaaaggtcg gcaaggaatt tgccgccgcc
     8281 tatgccttca aatgcggcgg cggcttcggg catgggcgtg cagaacgcat caaacacatc
     8341 ctgtccgact tcaaacgcat ccgcactgat ggcgggaccg agatacgcca tcatttcgac
     8401 aggcggaacc ttcatcgcgg caatggtgtt ttgcaatacg ccgcccgcca aaccgcgcca
     8461 gcccgcgtgt gcggcggcaa ccgccgtacc tgccctgtcg caaaatagaa cgggcagaca
     8521 gtctgcggtc atcacagcac agacggcttt acccgtgtcg tccacggaag cgtccgcatc
     8581 gggtgtgcct cccaacgatt ctgcagcgtt gacgacgacg gtgctgtgga tttgattgag
     8641 gtaggcaacg ggcaatccga cctgctgttg cacgatttcg cggttgcggc gcacggcttc
     8701 gggattgtcg ccgacgtgcg taccgaggtt caaactctga tacgcacctt ggctcacgcc
     8761 gccgttgcgc gtggtaatca gggttttcac attggcgggc gcgggccaat cggcggtcag
     8821 gaagtttttg cctttcgggg cgagatttag ggtttctgtg atggttttca ttggatattg
     8881 gaataggtgt gtggcggaaa gggttgatta actgccttcg gggcaaatgg atgcttgttt
     8941 tttcgtcatt cccgcacagg cgggaattcg gagattcggg attgttttca aacgttcggg
     9001 tttggattct tgccggtgca ggaatgatgc ccttatatac tttctccgag ctttatatgt
     9061 ttcaacaggg gatggcacat caagcaccgc actgcgtgtt gcccgaacga gactaccctc
     9121 cgattagatt ctatcgctat aaacagatgg gtttcaaccg aaaaggaatg gggataaagt
     9181 ccatttccga cacctctcgg gcgatgccgt ctgaaaacca atctccactt tcagacggca
     9241 ttgtttaggc acggtcattt tgcaaattaa ggttaaggcg tgtatgcttg ccttccctta
     9301 aaaacaaaca cgcaacgcat tattatgaat atcctcagta aaatcagcag ctttatcgga
     9361 aaaacatttt ccctctgggc cgcgctcttt gccgccgccg cttttttcgc gcccgacacc
     9421 ttcaaatggg cggggcctta tattccttgg ctgttgggca ttattatgtt cggtatgggt
     9481 ttgacgctca aaccttccga cttcgatatt ttgttcaaac atcccaaagc cgtcatcatc
     9541 ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct ggctgctgtc caaactgttg
     9601 aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg gctgctgccc gggcggtacg
     9661 gcttccaatg tgatggccta tctggcgcgc ggcaatgtgg ctttgtcggt tgccgttacg
     9721 tctgtttcca ccctgatttc cccattgctg actcccgcca tcttcctgat gcttgccggt
     9781 gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca tcgtcaaaat ggttttgctc
     9841 cccattgttt tgggtttgat tgtccataag gttttgggca gtaaaaccga aaagctgacc
     9901 gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga ttatcggcgc ggttgttggg
     9961 gcaagcaaag gcaagattat ggaaagcggc ctgctgattt ttgcggttgt cgtactccac
    10021 aacggcatcg gctacctgct cggcttcttt gccgccaaat ggaccggcct gccttatgat
    10081 gcacaaaaaa cgctggccat cgaagtcggt atgcaaaact cgggcctggc cgccgcgctt
    10141 gccgccgcac actttgccgc tgcgccggtc gttgccgttc cgggcgcatt gttcagcgtg
    10201 tggcacaata tctccggctc gctgctggca acttattggg cggccaaagc cggtaaacat
    10261 aaaaaaccct agaagccttt catcataatg ccgtctgaac ggggcaaccg aaaccggcgg
    10321 tttccgcccg accccttcag acgacctttg tttgaaatcg gcggggacga ttgtccggct
    10381 gctccgatgc ggctgcctgc gttcgccccg cctttcaagc cgcctactcc cttacataaa
    10441 ccacttcgac atcgtagtcg tcttcgttcc aatcgtcatc gtcgtccgcg ccgaatttgt
    10501 cctgccattc ctcttcattg ctcaaagacg aatccaaacc ggcttcaaga cggaggacag
    10561 acaacaaatg atatatgtcg tccggaatcg gtgcctcaaa cgaaacggtt tcgccgcttt
    10621 ccggatgggt gaaactcaag cggtaggcgt gcaacgcctg acgcgcgccc aaacttttaa
    10681 cggcttcttt caccgtgtcg ccgcacggat ggcgcgggtt gccgtaaacc gggtcggcgg
    10741 caagcggatg gttggcctcg cgcatatgga cgcggatttg gtgcgtcctg cccgtttcga
    10801 gcgagcattc gatatagctg tgggtaagat agcgttccaa cactttgacg tgggtaacgg
    10861 ctggtttgcc gccgaatttg acgactgcca ttttcaggcg gttgtgcgga tcgcgtccga
    10921 tttgggtttc gattttaccg tcgaagggga cgatgccgtt ggcgacggcg cggtagatgc
    10981 gtttgaccgt gcgctcttga agctgcctca cgagggaatt ttgcgccggc agggttttgg
    11041 caaccaccat cagcccgctg gtttccttgt ccaaacggtg tacgatgccc gcgcgcggta
    11101 cttggctcaa ttcgggacag tgcgccaaca ggccgttgag cagcgtcccc gtccagttgc
    11161 cggccgccgg atgcaccacc agtccggccg gtttgttgac gacgatgacg gtatcgtctt
    11221 cgtaaacgat atccaaagcc ataggctctg gaacaaacgc caaattttcc tcactcggac
    11281 ggacggttac acaaatttgc tcgccgccta tcattttgtc tttgggttgc gaaggtttat
    11341 cgtttacaat aaccgcgcct tctttaatcc atgatgtcag gcggctgcgc gagtagtcgg
    11401 gcagaagttt cgccaatacc gcatccaacc gcccgccggc aagctcgagc ggaacggtca
    11461 gaccgatacg gttttcagtt tcggaagctg acgcaaagtc tgaatcgtcg ctataatcgg
    11521 attcattatc aaaggaagta ttctgcatga aaaaaattct tttaacggtt tcattaggtt
    11581 tggcactgag tgcctgtgcc actcaaggta cggtcgataa agatgcccag attacccaag
    11641 attggagtgt ggagaagctc tatgccgaag cccaggacga attgaacagc agcaattata
    11701 cgcgggctgt caagttatac gaaatcttgg aatcgcgctt ccccaccagc cgccatgccc
    11761 ggcagtcgca actggatacc gcatacgcct attataaaga cgatgaaaaa gacaaggctc
    11821 tggcggcaat cgaacgcttc cgccgcctcc atccgcagca tccgaatatg gattacgcgc
    11881 tgtatctgcg cggcttggtg ctgttcaacg aagaccagtc cttcttgaac aaactggcct
    11941 cgcaagactg gtccgaccgc gacccgaaag ccaaccgcga agcgtatcag gcctttgcgg
    12001 aactcgtcca acgcttcccg aacagcaaat acgccgccga tgcaaccgca cgcatggtca
    12061 aactggtcga tgcactgggc ggcaatgaaa tgtcggtggc gcgctactac atgaaacgcg
    12121 gtgcatacat cgccgccgcc aaccgcgccc aaaaaatcat cggcagctac cagaatacgc
    12181 gttatgtcga agaatcgctc gccatattgg aacttgccta ccaaaaactc ggcaaaccac
    12241 agcttgccgc cgatacgcgc cgcgtgttgg aaaccaactt cccgaaaagc ccgtttttga
    12301 cgcacgcttg gcagcccgac gatatgccct ggtggcgtta ctggcattaa gcccatcttc
    12361 gtaatgccgt ctgaagccgt ttcagacggc attttatgtt tcaaacctca ttcaaacggt
    12421 tttttctgcc aatagaattt ataccctctc aaacgttgag ccttcacgcc gccgcgcccc
    12481 aagccgaatt gcagcgcgcc tgacaaatcg gtacgcagca gtttgatgcc gtgtgcgcgg
    12541 acacggttct gcaccgcttc ggtcggatgt ttgtaggcgt tggcataacc gcttgaagca
    12601 acagcatatt cgggcgagac ggcattgagg aacacgcccg acgaggacgt attgctgccg
    12661 tgatgtccca acaccaaaac ctggctgtac aggttgcctc catacttgcc gaccaggctt
    12721 tcctcgccct tcgtatccaa atcgcccgtt accagcagtg ccgcaccgcc cgccacaaca
    12781 cgcaaaacac aacttttccc attatcatcg atgtttttgc gttcagacgg catcaaaaac
    12841 tcaaaatcca cgccgtccca ttgccaacgc tgttccgcac aatgccgcgc cccctcataa
    12901 aattccggct gcccggcata aatcccgccg ttcggtatct tgccgactgc ctgaaaaccg
    12961 ccgtcgtggt cgctgtcgtg atgcgacaga accagcttgt ccaaacggcg gacacccgcc
    13021 gcattcaaac tcggcacaat ccccatctgc gccgccgctg ccgttccggt gtcaaacaaa
    13081 agatgatgat ttgccgtctg aaccgacacc gacaaaccct gccccgcatc ccaaaccgta
    13141 accgcaacct cattttccgg cacgccgggt gaacggtaaa acacaaaccc tgccaacagc
    13201 aacaccgccc acggacgcaa acccaagccg cgcggcagca gcaacagcaa agcggcacac
    13261 accgccaaca ccaacagcgg caaaggtgcg gaggcaacgg caaactcggg cgacacatcg
    13321 gcaagccaca ccaaaaaccg caaagtatat tccgccaaaa atgcccccaa ctgttgcaaa
    13381 ggcgcaaacg gcacgaccga acccagcaac gccagcggcg tcaataccca agaaaaccac
    13441 ggaattgcca ccgcattgac caaagggctg attaaaggca gcgaagcaaa cagataaccg
    13501 agcaaaacca gcgacaacac cgaagccgcc cactgcccgc gcaaagcagt ttgccgtttt
    13561 ccctcgtgca aacgccccga gcacgcccat atcagggccg ccaccaaacc gaaagacagc
    13621 caagtcccca cacccaagac cgccgacggg tcgaacagca gcaccgccgc caacgcctgc
    13681 caccacgtcg cccacgccga caatcttccc ctgcgccaag cccacgcaaa cgccgccaac
    13741 atcaaaacgc tgcgctgcgt cggcacggaa aaaccggcaa gcagcgcgta aaacagcgcg
    13801 cctgcacacc ccgccgccaa aacccacacg cgcggccggg caggaaggcg cggggaacag
    13861 gcaagcagcc gcttcgccag ccacgcaaac aacaccgcca ccatcgtaac gtgcaaaccc
    13921 gaaatactga ccaaatgcgt cagccccaac ggccggaacg cctgccacaa ttcggggcgc
    13981 aatgccgact gttcgcccac gctcaacgcg cgcatcagcc cgatgccgtc tgaaagcccg
    14041 ccgtccgcat ccgcctgctg ccaattacgg ctgatgcggc tgcgccaaac cgcaatcccc
    14101 caaccgcttc cgccatgcag caaaaccctg tccgcaccga ccgtccccgc gccgcctatt
    14161 ccgttggata atgcccacgc ctcacgattc aaaccgcgca ggttcaactc cccgacgaca
    14221 gggtgcacac gtgccgttat ccgccatctg ctcccgaccg cccattcgcg ccgtttgtag
    14281 tccgacagca gcaaatcaaa cgtccgacca ccgctgtcca cagcctttgc cgcaaactgc
    14341 acacgccgcc cgtccgacct cggcatatcc gccacttcca ccgtcaaagg cacgcctgaa
    14401 acggcttctg cccgccattg cgaagacagt gccgcttccg ttctgaatac gccgtaagcc
    14461 gcccccacca acacgcacag catcagaccg gcaaacgcaa aacgccttgc aaacacagca
    14521 aaaaccgcaa aagccgccag ccaaaacagc caatgcggca cgaccggcag cgcaaacgaa
    14581 gccgccgcac cgacaaccca acacggcaaa aaccaacgac ggaacacgtc tccccacccc
    14641 agatatttca aaaataaacg gataataaag caaatccgaa aaaccgtcat aacaagattt
    14701 gaaaatgata tagtggttta aatttaaatc aggacaaggc gacgaagccg cagacagtac
    14761 aagcagtacg acaaggcgag gcaacgctgt actggttttt gttaaaccac tataagaatg
    14821 caaacgaaaa gcctcagtat tatccgagac aacggcaaac tttccatcgt cattcccacg
    14881 aacccacatc ccgtcattac cacgaaagcg ggaatctagg acgcagggtt aagaaaacct
    14941 acatcccgtc attccctcaa aaacagaaaa ccaa
//
LOCUS       DAWFJW010000042        14897 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFJW010000042 DAWFJW010000000
VERSION     DAWFJW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.042"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(105..1430)
                     /locus_tag="WGR19_001053"
     CDS             complement(105..1430)
                     /locus_tag="WGR19_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0798596.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1641..2480
                     /locus_tag="WGR19_001054"
     CDS             1641..2480
                     /locus_tag="WGR19_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0798597.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2477..3643
                     /locus_tag="WGR19_001055"
     CDS             2477..3643
                     /locus_tag="WGR19_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0798598.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3907..5172
                     /gene="efeB"
                     /locus_tag="WGR19_001056"
     CDS             3907..5172
                     /gene="efeB"
                     /locus_tag="WGR19_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0798599.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5244..5573)
                     /locus_tag="WGR19_001057"
     CDS             complement(5244..5573)
                     /locus_tag="WGR19_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0798600.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5650..7020)
                     /gene="glmU"
                     /locus_tag="WGR19_001058"
     CDS             complement(5650..7020)
                     /gene="glmU"
                     /locus_tag="WGR19_001058"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0798601.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPNDPTGLGRIIRDSNGSVTAIVEEKDADAV
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNQIGPYARLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDGKLALARSRQTIIDGWVR
                     PEKNKQ"
     gene            complement(7075..7347)
                     /locus_tag="WGR19_001059"
     CDS             complement(7075..7347)
                     /locus_tag="WGR19_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0798602.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7323..8054)
                     /locus_tag="WGR19_001060"
     CDS             complement(7323..8054)
                     /locus_tag="WGR19_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0798603.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQEYWHHYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPRKYERMLCVKPSLS"
     gene            8113..9114
                     /locus_tag="WGR19_001061"
     CDS             8113..9114
                     /locus_tag="WGR19_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0798604.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGMGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9208..10056
                     /locus_tag="WGR19_001062"
     CDS             9208..10056
                     /locus_tag="WGR19_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0798605.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10082..10567
                     /locus_tag="WGR19_001063"
     CDS             10082..10567
                     /locus_tag="WGR19_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0798606.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10669..12237)
                     /gene="msrAB"
                     /locus_tag="WGR19_001064"
     CDS             complement(10669..12237)
                     /gene="msrAB"
                     /locus_tag="WGR19_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0798607.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12383..13648
                     /gene="ftsY"
                     /locus_tag="WGR19_001065"
     CDS             12383..13648
                     /gene="ftsY"
                     /locus_tag="WGR19_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0798608.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETAQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            14567..14851
                     /locus_tag="WGR19_001066"
     CDS             14567..14851
                     /locus_tag="WGR19_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0798609.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT         3250 a         3729 c         4434 g         3484 t
ORIGIN      
        1 ttccgtcaaa taagaaagga attttgtgcc tgcggtatcg caaaacttcg ccttaatgcg
       61 cccgattgcc tagggatggg cttcagatgg cattgttttc cggtttacgg gcggtattcg
      121 ggcttcatac cgttgggcag aagctgccag acatatcccg tggttttctg tttgccggca
      181 agttcgccgg cttcgtcgcc gtaaccccag aaataatcca cgcgcaccgc gcctttaatc
      241 gcgctgccgg tatcctgcgc cataatcagg cggttgaggg ctttgcgggt aaccggatgg
      301 gcggtggcga caaataaggg cgcgcccaag gtaatgtagt gccggtcgac tgcgccggcg
      361 tactcgccca tcagcggcgt gcccagtgcg ccgacagggc cgtcattgct gcttccggta
      421 agctctcgga aaaagatata gctggggttt tgacccaaaa cttcggcgag gcgttgcgga
      481 ttttgccgca tataagactt aatgccctgc atggaggttt gcccgagttt gaggtagccc
      541 ttatccgcca tgtattttcc gatggaaaca tacggatgtt cgtttttgtc ggcatagccg
      601 atgcggatgt atttgccgga cggggttttc agacggcccg agccttgtat gtgcataaaa
      661 aaaagttcga cggggtcttc ggcgtaaccg agtatcgggg ctttgccgtc aagcgcgccg
      721 ccgttgattt ggttgcgcgt gtggtagggg aggaagcggc ttccttcaaa cctgcctttg
      781 attgccgttg tgcgcgcggt gatggggaat cgggagaggt cggcggtatg tgtgccgccg
      841 gtattgtcga ttgtgccgct gtttttcccc gtctgcctga tgcggacaag ggcttttccg
      901 ctccgcaaac cggcaggcag ggggacggag ataaaatcgt cgggaatacc gtaaatcggg
      961 aagcgggctt gtgccgtccg cctgtcgtcg cctttcagca ccggttcgta atagccggta
     1021 accgtaccgg caaggcttcc gttgcctgca acctgccacg gcgtgaaata gcgttcaaaa
     1081 aactgttttg cctgaaagga atggacgggg gtttgaaagg cttgggcgca cacatcctgc
     1141 cagccttggc ggtttttcaa attggcgcag ccgaggcgga aggattgcag gcttttggcg
     1201 aaatcctgcg ccgcccagtg gggcagggac aggtgcggca caacggtata aacggccccg
     1261 ccgccgccga ccgtcgttcc ggcggggtcg gggatgccgg ccggccggtc cgggccgttg
     1321 atgacggatg tgtcgggttg cggaaaggtt tggatgctct tgctttggca ggcggcgagg
     1381 atggcggcgg cgatgccgca cagggcggcg cggaacaggt attttttcat aatggacaat
     1441 gttgccggca gtaataagaa agatggtttc gggcggcgtt gcggcagccg tggagagggg
     1501 attttaacac agggcgcagc tgcagcctgc ggaactttcc gccgcgcggt actgcagata
     1561 aaaataactt gcatttgtat ttacaagcaa tgaaaatatt ccgataatat attattcatc
     1621 atccttgttc gttcgcgttt atgctggtcg cttttttaat tatgttgcgc gagggtattg
     1681 aagccgcgct cattgtcggc atcgttgccg gttttctgaa acagtccgga cattccaaac
     1741 tgatgcctaa ggtctggttc ggggtcgtcc ttgcttcttt gatgtgtttg gggctggggt
     1801 acggcatcca ttcggcaaca ggcgagattc ctcagaagca gcaggagttc gtcgtcggca
     1861 ttatcggttt ggttgctgtc gcgatgctga cttatatgat tttatggatg aaaaaggcgg
     1921 cgcgttcgat gaagcggcag cttcaggatt ctgtacaggc ggctttgaac cgtggcagcg
     1981 gtcaaggatg ggccttggtc ggtatggcgt ttcttgccgt ggcgcgcgaa ggtttggaga
     2041 gcgttttttt cctgcttgcc gtattcaaac agagcccgac gtggcagatg ccggccggtg
     2101 cggtagcggg ggttttggct gccgccgtga ttggcgcgtt gatttatcag ggtgggatgc
     2161 gcctgaatct ggctaagttt ttccgttgga cgggggcgtt tttgattgtc gttgccgccg
     2221 gcctgcttgc cggctcgctg cgcgcgctgc acgaagcagg catttggaac gcgcttcagg
     2281 acattgtgtt cgactcatca aaatatttgc acgaagacag tccgttaggt gtgctgctcg
     2341 gcggattttt cggctatacc gaccatccga cgcagggcga ggccttggtt tggctgctgt
     2401 accttattcc cgtcatgact tggtttttgt gcggcagcag gccgtctgaa actttaaccc
     2461 gtaaagagga gttgaaatga gaaaattcaa tttgaccgca ttgtccgtga tgcttgcctt
     2521 aggcttgacc gcgtgccagc cgccggaggc ggagaaagct gcgccggccg cgtccggtga
     2581 ggcgcaaacc gccaacgagg gcggttcggt cagtatcgcc gtcaacgaca atgcctgcga
     2641 accgatggaa ctgaccgtgc cgagcggaca ggttgtgttc aatattaaaa acaacagcgg
     2701 ccgcaagctc gaatgggaaa tcctgaaagg cgtgatggtg gtggacgagc gcgaaaacat
     2761 cgcccccgga ctttccgata aaatgaccgt caccctgttg ccgggcgaat acgaaatgac
     2821 ttgcggtctt ttgaccaatc cgcgcggcaa gctggtggta accgacagcg gctttaaaga
     2881 caccgccaac gaagcggatt tggaaaaact gtcccaaccg ctcgccgact ataaagccta
     2941 cgttcaaggc gaggttaaag agctggtggc gaaaaccaaa acctttaccg aagccgtcaa
     3001 agcaggcgac attgaaaagg cgaaatccct gtttgccgac acccgcgtcc attacgaacg
     3061 catcgaaccg attgccgagc ttttcagcga actcgacccc gtcatcgatg cgcgtgaaga
     3121 cgacttcaaa gacggcgcga aagatgccgg atttaccggc tttcaccgta tcgaatacgc
     3181 cctttgggtg gaaaaagacg tgtccggcgt gaaggaaatt gcagcgaaac tgatgaccga
     3241 tgtcgaagcc ctgcaaaaag aaatcgacgc attggcgttt cctccgggca aggtggtcgg
     3301 cggcgcgtcc gaactgattg aagaagtggc gggcagtaaa atcagcggcg aagaagaccg
     3361 gtacagccac accgatttga gcgacttcca agccaatgtg gacggatcta aaaaaatcgt
     3421 cgatttgttc cgtccgctga tcgagaccaa aaacaaagcc ttgttggaaa aaaccgatac
     3481 caacttcaaa caggtcaacg aaattctggc gaaataccgg actaaagacg gttttgaaac
     3541 ctacgacaag ctgggcgaag ccgaccgcaa agcgttacag gcctctatta acgcgcttgc
     3601 cgaagacctt gcccaacttc gcggcatact cggcttgaaa taagccgcaa gcgttcagac
     3661 ggcatttggc ggcagatacc gtctgaagtt ttatagtgga ttaacaaaaa ccagtacggc
     3721 attgcctcgc cttgccttgc cgtactattt atactgtctg cggcttcgtc gccttgtcct
     3781 gatttttgtt aatccactat atccgccata tattgcaggg cgggatttca acctgccgct
     3841 atcggttaat ggaaaaacgg cgtgcaggga tacccatcct gctgcacgga tattgaagga
     3901 aacaccatga gcaaaaacca acccgcacaa ccgaccaggc gcactctttt taaaaccgcg
     3961 atcgcagccg gagcagtcgg cgcaatcgga ggttatctcg gcggcaaaaa acagggcgaa
     4021 accgccgaac gcaccgccga aagccaacac tcgccccaag cctatccctg ctacggcgaa
     4081 catcaggcag gcatcgttac gccgcagcag gcgttttcga ttatgtgcgc cttcgacgta
     4141 accgcgcaaa gtgccaagca gctggaaaac ctgttccgca cgctgaccgc ccgcatcgag
     4201 tttctcaccc aaggcggcga ataccaagac ggcgacgaca aacttccgcc agccggcagc
     4261 ggcattttgg gcaaagcctt caaccccgac gggttgaccg ttaccgtggg ggtgggcagc
     4321 agcctgtttg acggccggtt cggactcaaa gacaaaaaac cgattcattt gcaggaaatg
     4381 cgcgacttct ccaacgataa gctgcaaaaa agctggtgcg acggcgattt gagcctgcaa
     4441 atctgtgcct ttacccccga aacctgccaa gccgccctgc gcgacatcat caaacacacc
     4501 gtccaaaccg ccgttatccg ttggagtatc gacgggtggc agcccaaatc cgaacccggc
     4561 gcgatggcgg cgcgcaacct gttgggcttc agggacggca cgggcaaccc caaagtttcc
     4621 gaccccaaaa ctgccgacga ggttttgtgg acgggtgtgg ccgccaacag cctcgacgaa
     4681 ccggagtggg cgaaaaacgg cagctatcag gcagtccgcc ttatccgcca ctttgtcgag
     4741 ttttgggaca ggacgccgct tcaagagcaa accgacattt tcgggcggcg caaatacagc
     4801 ggtgcgccga tggacggcaa aaaagaagcc gaccaaccgg attttgccaa agaccccgag
     4861 gggaatacca cgcccaaaga cagccatata cgcctggcga atccgcgcga tcccgaattc
     4921 ctcaaaaaac accgcctctt ccgccgcgcc tacagctatt cgcgcggact cgcctcaagc
     4981 ggacagcttg atgtcggact ggtgttcgtc tgctatcagg caaaccttgc cgacggattc
     5041 atcttcgtgc aaaacctcct caacggcgaa ccgctggaag aatacatcag ccccttcggc
     5101 ggcggctatt tcttcgtctt gcccggcgtg gaaaaaggcg gctttttggg gcaagggctg
     5161 ctgggcgtat aaatccgcca tataaaaaac gccgtccgaa ccttgccaaa cgggttcgga
     5221 cggcgtttct tgtttttggg cggtcaggct tttttgacga attcggattt taaattcatc
     5281 gcgctgccgt cgattttgca gccgatgttg tgatcgcctt cttgcaggcg tatgcctttg
     5341 acttttgtgc cttgtttgat caccatcgag ctgcctttta ccttgaggtc tttgatgagg
     5401 atgacggtat cgccgttttg cagcactgcg ccgttggcat cgagcacttg agccgcaagg
     5461 tcggcggcgg attcggtttc attccattcg tgggcgcatt cggggcagat gtattgtccg
     5521 ccgtcttcat aagtgtattc ggaggcgcat tgtgggcatg ggggtaatga catggtttgc
     5581 agtccttatc tgatatcgga ggagtttgat gccgtctgaa acctgaaacc ggcttcagac
     5641 ggcatagctt tattgtttgt ttttttcagg cctcacccag ccgtcgatga tggtttggcg
     5701 ggagcgggcg agggcgagtt tgccgtcttc gacattgcgg gtaatcgtgc tgcccgcgcc
     5761 ggtggttact ttgttgccga ttttgacggg ggagaccaag acgcagtttg aaccgatgcg
     5821 cacttcgtcg ccgatgacgg ttttgtgttt gtgcacgccg tcgtagttgg caataatcgt
     5881 acctgcgccg aagttggttt tgctgccgac ttcggcatcg ccgatgtagg tgaggtggtt
     5941 ggctttggtg cctttgccga tggcggcgtt tttgatttcg acgaagttgc cgacgtgtac
     6001 gtcgttagct agtttggctt gcggacgcaa acgggcgtat gggccgattt ggttgttttc
     6061 gccgacttcg cagtcttcga ggtgggagaa gggggcgatt ttgctgtttg cgccgatttt
     6121 ggcgtttttg atgacgcagt ttgcgccgat ttcgacgttg tcgccgattt cgacttcgcc
     6181 ttcaaagata cagttcacat caattacgac atcctgtccg tgtttcagac ggcctcgtaa
     6241 atcgaaacgt gccggatcgc gcagggttac gcctgctttg agcaattctt gtgcctgttc
     6301 ggtttggaag atgcgttcga gttcggcgag ctggcgtttg ttgttcacgc cggcggcgag
     6361 gtgggaggcg cgcactcgga cgggatgaac tttaataccg tcggcaacgg ctttggcgat
     6421 gaggtcggtt aaataatatt cgccttgggc attgttgctg gaaaggctgt tcagccagtt
     6481 ttccagtttg gcgttgggca ggacgagaat gcctgtgttg gtttctttca cggctttttg
     6541 gacggcgtcg gcgtcttttt cttcgacgat ggcggttacg ctgccgttgc tgtcgcggat
     6601 gatacgcccc aagcctgtcg ggtcgttggg aacgtcggtc aacagcccga cttcgttgcc
     6661 tgcggcttcg agcagggttt cgagggtttc aacgtcaatt aaaggaacgt cgccgtacaa
     6721 caccagcgtg cggccttcgg cggcaaggtg gggcagggcg gttttgacgg cgtggccggt
     6781 gccgagctgt tcggtttgtt caacccagac gacatcgcgt ttgacggtgt ccaaaacttg
     6841 ctctttgccg tggccgacga cgacgcagat gttttgcgga ttcagcgcgg cggcggtgtc
     6901 gataacgcgc ccgagcattg tttcgccgcc gatttcgtgc aaaaccttgg gcattttgga
     6961 atacatgcgc gtgcctttgc cggcggcgag gatgacgatg tttaaagtgt tttgcggcat
     7021 agcgttttcc tgtgcaatgc cgtctgaaag ggcttcagac ggcatagggt gggtttatcg
     7081 gttttgaaat ttgggctttt gccagtgttg gcgatgctct tcgtcggcgt tgttgccggt
     7141 ttggttgggt aacacggcat ggcgttcggg acggtattgg ttgtagttca tatttttcga
     7201 gtagctgccg tcttggtaat aaacgggcgt gccggcggga tatttttgac ggacggcggt
     7261 cttgccgttg ccgtcttgat aagtttccca cgcgcagccc gacaaaaggg cggcggcagt
     7321 catcaggata aggaaggttt tacgcataac attctttcgt attttcgggg gggtgtgcgg
     7381 gtattgtaat gattttggcg gtgttctgac aaagtttctg cataccgagc cagttgcgcc
     7441 atatcgctta cggaagcatc gataaagggc aggtcgtgca cttttgcacc gaaccggacg
     7501 gttttcatgc ccaatgcttt tgcctgatgc aggttgtccg cgctgtcgtc caccataatg
     7561 cagcattcgg gcggtacgtc caaaaggcgg cagacattga gatacgcttg cggattgggt
     7621 ttgtacagca gcccgaaatc atccgtgccg aacagtgcgt cgaaacggtt ttccaaaccg
     7681 agtgcgccga caacggcacg gacgtaaaac gacgggccgt tggaaaaaac cgccttgcgc
     7741 ccttttaggc ggctcagggt gttttgtgtt tccggcatac cgtgcagacg ggtcaaaacc
     7801 tcgtcaatcg gatggctttc gcgcaaaaat tcggcaatgt cgatttcggg atggtggatt
     7861 tgcagtccgg cgagcgttgc gccgtagtgg tgccaatatt cctgacgcag gtcggacgcg
     7921 gcggattcgg agagtttgag gcggcgtgcc atatagcgtg tcatagcgcg gttgatgagg
     7981 tggaagatgc cggcatcggc attgtgcagc gtgttgtcga ggtcgaacag ccacacggtc
     8041 gggttttctt gcatgttgaa ccgtgaaaat ttgttagaat gttattttac agcgaataga
     8101 ggaggactcg gaatgaaacg gaaaatttgg ctgctgccgc tgctggcggt ttcggcatac
     8161 ctgcaggcgc agacggaagt caggctggcg gtgcataagt cgttcagcct gcccaaaggg
     8221 ttgattgcgc gcttcgagcg ggcaaacgat gcgaaggtgt cgattattca ggcgggcggc
     8281 gcgaacgaaa tgctcaacaa actgattttg agccgcgcca acccgattgc cgacgcggtg
     8341 tatggtttgg acaacgccaa tatcggcaag gcgcgggaaa tgggcatttt ggcggcggcg
     8401 caacccgaat ccgcccccgt cgcggtcggg ctgccttcgg ctttggcggt cgattacggc
     8461 tatgtgtcca tcaattacga caaaaaatgg tttgaaggca aaaagctgcc cctgccgcaa
     8521 accctgcagg atttgacccg ccccgaatat aaaaacctat tggtcgtgcc gtcccccgcc
     8581 acgtcgtccc cggggctggg cttcctgatg gcgaacatca gcgggatggg cgaagaaggc
     8641 gcgttcaaat ggtgggcaca gatgcggcag aacggcgtga aggtcgccaa aggctggagc
     8701 gaggcgtatt acaccgactt ttcgcacaac ggcggcgcgt atccgctggt ggtcggttat
     8761 gccgccagtc cggcggcgga agtgtatttt tccaaaggca aatacagcga gccgccgacg
     8821 ggcaacctgt ttttaaaagg cggcgtattc cgccaggtcg aaggcgcggc ggtcttgaag
     8881 ggcgcgaaac agccgaagct ggcggcaaaa ctggtgcaat ggctgcaaag tcgggaagtg
     8941 cagcaggcgg ttccgtccga aatgtgggtt taccccgccg tcaaaaacac gcgcctgccc
     9001 gacgtgttcc gcttcgccca agccccgacg cacaccaccg cccccgcgca gcgcgatatt
     9061 gatgcgaacc agcgcggatg ggtttcccgt tggattagaa cggttttgaa ataaaacaaa
     9121 cataccgccc cgcagggctt catacggcat ttttacacct gtgccgatta cgccgcacgg
     9181 ggcggatgtt cgatcaagag gaaaacaatg gacttcaaac aatttgattt tttacacctg
     9241 atcagtgttt ccggttggga gcatctggct gaaaaggcgt gggcgttcgg gctgaacctt
     9301 gccgccgcgc tgcttatttt tttggtcgga aaatgggcgg cgaaacgcat tgtcgctgtg
     9361 atgagggcgg cgatgacgcg cgcgcaggtc gatgccacgc tgattagttt tttgtgtaat
     9421 gttgccaata tcggcttatt gattttggtg attattgccg cattgggcag attgggcgtt
     9481 tccacaacat ccgtaaccgc cttaatcggc ggcgcgggtt tggcggtggc gttgtccttg
     9541 aaagaccagc tgtccaattt tgccgccggc gcgctgatta tcctgttccg cccgttcaaa
     9601 gtcggcgatt ttatccgcgt cggcggtttt gaaggatatg tccgagagat taaaatggtg
     9661 cagacttctt tgcggacgac cgacaacgaa gaagtcgtgc tgcccaacag cgtggtgatg
     9721 ggcaacagca tcgtcaaccg ttccacactg ccgctgtgcc gcgcccaagt gatagtcggc
     9781 gtcgattaca actgcgattt gaaagtggcg aaagaggcgg tgttgaaagc cgccgtcgaa
     9841 caccccttga gcgttcaaaa cgaagagcgg caggccgccg cctacatcac cgccttgggc
     9901 gacaatgcca tcgaaatcac attatgggct tgggcaaacg aagcagaccg ctggacgctg
     9961 caatgcgact tgaacgaaca agtggtcgaa aacctccgca aagtcaatat caacatcccg
    10021 ttcccgcaac gcgacataca catcatcaat tcttaaacgc cgtctgaaag aggagtggga
    10081 aatggacgcg ctgcacacca tcgcccgaaa cctgacgaaa aaacgtcaaa ccgtaagctg
    10141 tgccgaatcc tgcacgggtg ggctgcttgc cgccgtattc acaagcgttg caggcagttc
    10201 gcaatggttc gatcaaagtt ttgtaacata cagcaacaaa gccaaagaag accgcttggg
    10261 cgtgttgccc gaaaccctgc tcgaacacgg cgcggtcagc cgccaaaccg tctatgagat
    10321 ggcgcgcggc gcgaaagccg tggcgcaggc ggattacgcc gtcggtattt ccggcatcgc
    10381 cggtccgggc ggcggcagcg aaagcaaacc cgtcggcacg gtttggttcg ggtttgcctt
    10441 tccgggcgga agttgcgaag caatgcgccg ttttgacggc aaccgcgaat ccgtccgcgc
    10501 gcaggcggtc gccttcgcgt tggaacggtt ggcggggctg attgaaaacg gcggcgatgc
    10561 tgtctaaaca aaatctccgt ctggacagaa tccccgccgg ataaaaaata ccgtctgaaa
    10621 cgttgcaggt ttcagacggt attttgtcgg gttaggcggc ggtgcggctt atttcacttt
    10681 actcttcaac gcgccgtagc ctgccgcgtc catttgttcc agcgggatga atttcaagct
    10741 cgcgccgttg atgcagtagc gcagtccgcc tttgtcgcgc gggccgtcgg ggaagacgtg
    10801 tcccaaatgc gaatcggcgg cgtggctgcg cacttcggtg cggcgcatgt tgtagctgaa
    10861 atcatcgtgt tcggtaacgg attttgcatc aatcgggcgc gtgaagctcg gccagccgca
    10921 gccggaatca tatttgtcgg cggagctgaa caaaggttcg ccgctgacaa cgtccacata
    10981 aatgccgggt ttgaacaaat ggtcgtattc gtggctgaag gcatattcgg tcgcgctgtt
    11041 ttgggtaact tggtattgct cttcggtcag ggtgcgtttg agttcggcgt cactcggttt
    11101 tttatacgtt gccgcatcga agcctttgcc ttgcggggcg gtcttggttt tgcccggcag
    11161 cggttcgtcg gctttgcgga tatcgatgtg gcagtagccg ttggggtttt taatcaggta
    11221 gtcctgatgg tattcctcgg catcgtagaa gtttttcagc ggctcgtttt caacaacgag
    11281 gggcagttgg tatttttgct gctcgcgttt gagggcggcg gcgatgacgg ctttttcggc
    11341 ggggtcggtg tagtacacgc cgctgcggta ttgcgtaccg gtgtcgttgc cctgtttgtt
    11401 gaggctggtc ggatcaacga cgcggaagaa atattgcagg atgtcgtcta ggctgagttt
    11461 gtcggcatcg taggtcactt tgacggtttc ggcgtggccc gtatggcggt aggacacgtc
    11521 ttcatagctc ggatttttcg tgttgccgtt ggcgtagccg gataccgcgt caaccacgcc
    11581 gtcgatgcgt tggaaatagg cttccaagcc ccagaagcag ccgccggcga ggtagatggt
    11641 gcgcgtgttc atgatttttg aatccttttt ctgagtgtcg ggtttgtaga acgaatgttt
    11701 caagctgccc aaatcggcat tcgggtcgcg gattaacgcc aacgcctgcg cttcgttgat
    11761 gctgcctttg acgatgcgct gcacgtcgcc gtctttacca attaacgccc acgaggggta
    11821 aacgctgata ttcaggcttt gggcgatggt gccgccgttg tcggtaacga cgggcagctt
    11881 tggatagttc aaaccggcat accatttttg gaagtcgccg tcttttttct cgtgcaaaaa
    11941 gcccggagac gcgacggtaa tcaggttggc ggagctgaat tttgcatctt gcgcccattt
    12001 ttcggtctgt cccaattcgg acagacacaa aggacaccag ctcgcccaaa atttaatcag
    12061 cgtcggtttg tcttttttca agtaaacact ggcggggcgg ttgtctgcgg ttttcaaagt
    12121 ggataaagtg tgcggcacgg tcgcggctcc ggcatcgacg attttgggcg aacaagcacc
    12181 cagcgcaagc aggcagccga acttggcgca aagggaaaag aaagtacggt gtttcatttt
    12241 aatgtttcct gtgtggacgg tttgcatgat tagacgtttg agatgccgaa accttacagc
    12301 tcggattttc agacagcctt gccgcgtaaa atacgctaca atacgcccta tttcaagttt
    12361 ctaaaattaa aaggaaaatt caatgttcag cttcttccgt cgcaagaaaa aacaggaaac
    12421 gccggctctc gaggaggctc aaattcagga aaccgcagca aaagcagagt ccgaaaccgc
    12481 tcaagtaatt gaaaatatta aagaagatgc tgaatcttta gcagaaagcg tcaaagggca
    12541 ggtcgaatcg gctgttgaaa ccgtcagcgg tgcggttgaa caagtaaagg aagccgttgc
    12601 cgagatgctg tctgaagcag aggaagcggc ggaaaaagca gcggaacaag tcgaagcggc
    12661 aaaagaagcc attgccgaaa ccgtcggcga ggctgtcggg caagttcaag aagccgttgc
    12721 gacaactgaa gaacacaagc tcggttgggc ggcgcgtttg aaacaaggtt tgaccaaatc
    12781 gcgcgacaaa atggcgaaat cgctggcggg cgtgttcggc ggcggacaaa tcgacgaaga
    12841 tttgtacgaa gagctggaaa ccgtgctgat taccagcgac atgggcatgg aagccaccga
    12901 atacctgatg aaagacgtgc gcgaccgcgt cagcctcaaa gggctgaaag acggcaacga
    12961 attgcgcggc gcgttgaaag aagccttgta cgacctgatt aagcctctgg agaaaccgct
    13021 ggttttgccc gaaaccaaag agccgtttgt catcatgctt gccggcatca acggcgcggg
    13081 caaaaccacg tctatcggca aactcgccaa atatttccaa gcgcagggca aatccgtatt
    13141 gctggcggca ggcgatactt tccgtgccgc cgcgcgtgag cagcttcaag cttggggcga
    13201 gcgcaacaac gtaaccgtga tttcgcaaac cacgggcgat tccgccgccg tgtgcttcga
    13261 tgccgtccaa gccgccaaag cgcgcggcat cgacattgtg cttgccgaca ccgccggccg
    13321 cctgcccacg cagcttcact tgatggaaga aatcaaaaaa gtgaaacgcg tgctgcaaaa
    13381 agccatgccc gacgcgccgc acgaaatcat cgtcgtgctt gatgccaaca tcgggcaaaa
    13441 cgccgtcaac caagtcaaag cctttgacga cgcattaggg ctgaccggtt taatcgtgac
    13501 caaactcgac ggcacggcaa aaggcggcat cctcgccgcg cttgcctccg accgccccgt
    13561 ccccgtccgc tatatcggcg tgggcgaagg catagacgat ttgcgcccgt ttgacgcgcg
    13621 cgcgtttgtg gacgcactgc tggattgagc cgaaatgccg tccgaaaaca gcagaccgat
    13681 gccgtcattc ccgcgcaggc gggaatccag accttgggat aacagcaata ttcaaaggtt
    13741 ataaaagacc cgtcattccc gcgcaggcgg gaatctagac cttgggataa cagcaatatt
    13801 caaaggttat aaaagacccg tcattcccgc acaggcggga atctagaacc atcggcacgg
    13861 aaacttatcg ggtaaacggt ttcttgagat tccgcgtcct agattcccac tttcgtggga
    13921 atgacgggat tttaggtttc tgattttggt tttctgtttt tgtaggaatg atgaaatttt
    13981 gagttttagg aatttatcgg aaaaaacaga aaccgctccg ccgtcattcc cgcgtaggcg
    14041 ggaatctagg ccattggaca gcggcaatat tcaaagatta tctgaaagtc cgagattcta
    14101 gattcccact ttcgtgggaa tgacgggatg taggttcgtg ggaatgacgc ggtgcaggtt
    14161 tccgtgcgga tggattcgtc attcccgcgc aggcgggaat ctagacctta gaacaacagc
    14221 aatattcaaa ggttagctga agctttagag attctggatt cccactttcg tgggaatgac
    14281 ggaatgtagg ttcgtgggaa tgacgggatt tgagattgcg gcatttatcg gaaaaacaga
    14341 aaccgctccg ccgtcattcc cgcgcaggcg ggaatctagg tttgtcggtg cggaaactta
    14401 tcgggtaaaa cggtttcttt agattttgcg ttctagattc ccactttcgt gggaatgacg
    14461 aagagttgcg ggaatgatgg aaagctatgg gaataacgaa gggttaaagt aatcacggga
    14521 tggtgttcgc gggaatataa atttaaataa ttcaaaaggg tattatatgc agcctgcggt
    14581 ttatatttta gcaagccaac gtaatggcac gttatacatt ggcgttacat ctgatttggt
    14641 gcaacgtatt taccaacata gggagcattt gattgaggga tttacatcac ggtacaacgt
    14701 tactatgctg gtttggtatg aactgcatcc tacgatggag agtgcaatta ctcgggaaaa
    14761 acagttgaag aaatggaaca gggcttggaa attgcaactg attgaagaaa ataatgtttc
    14821 ttggcaggat ttatggtttg atattattta gcccgtcatt cccgcgcagg cgggaatccg
    14881 gcttgttcgg tttcggt
//
LOCUS       DAWFJW010000043        14846 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFJW010000043 DAWFJW010000000
VERSION     DAWFJW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.043"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WGR19_001067"
     CDS             complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WGR19_001067"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0798610.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1275..1880)
                     /locus_tag="WGR19_001068"
     CDS             complement(1275..1880)
                     /locus_tag="WGR19_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798611.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WGR19_001069"
     CDS             complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WGR19_001069"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0798612.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2865..3161
                     /locus_tag="WGR19_001070"
     CDS             2865..3161
                     /locus_tag="WGR19_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798613.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3175..3843
                     /locus_tag="WGR19_001071"
     CDS             3175..3843
                     /locus_tag="WGR19_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0798614.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3916..6036)
                     /gene="pnp"
                     /locus_tag="WGR19_001072"
     CDS             complement(3916..6036)
                     /gene="pnp"
                     /locus_tag="WGR19_001072"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0798615.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTSV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6273..7136)
                     /locus_tag="WGR19_001073"
     CDS             complement(6273..7136)
                     /locus_tag="WGR19_001073"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0798616.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERSIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDENGRLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7334..8197
                     /gene="rfbD"
                     /locus_tag="WGR19_001074"
     CDS             7334..8197
                     /gene="rfbD"
                     /locus_tag="WGR19_001074"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0798617.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSATIIRLLQHSNPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(8691..8924)
                     /locus_tag="WGR19_001075"
     CDS             complement(8691..8924)
                     /locus_tag="WGR19_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0798618.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLDVLDVDDDPVLE
                     EKYNELVPVLLAGDKEICHWYLDEKKLRQVLES"
     gene            complement(9090..9290)
                     /locus_tag="WGR19_001076"
     CDS             complement(9090..9290)
                     /locus_tag="WGR19_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0798619.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNVQPTVTAVINVQA"
     gene            complement(9462..10337)
                     /gene="xerD"
                     /locus_tag="WGR19_001077"
     CDS             complement(9462..10337)
                     /gene="xerD"
                     /locus_tag="WGR19_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0798620.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(10389..10829)
                     /locus_tag="WGR19_001078"
     CDS             complement(10389..10829)
                     /locus_tag="WGR19_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0798621.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            11150..12088
                     /locus_tag="WGR19_001079"
     CDS             11150..12088
                     /locus_tag="WGR19_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0798622.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            12217..12510
                     /gene="hfq"
                     /locus_tag="WGR19_001080"
     CDS             12217..12510
                     /gene="hfq"
                     /locus_tag="WGR19_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0798623.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            12595..13161
                     /locus_tag="WGR19_001081"
     CDS             12595..13161
                     /locus_tag="WGR19_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0798624.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            13215..13589
                     /locus_tag="WGR19_001082"
     CDS             13215..13589
                     /locus_tag="WGR19_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0798625.1"
                     /translation="MQYLFVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            13599..14093
                     /gene="folK"
                     /locus_tag="WGR19_001083"
     CDS             13599..14093
                     /gene="folK"
                     /locus_tag="WGR19_001083"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0798626.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(14220..14699)
                     /locus_tag="WGR19_001084"
     CDS             complement(14220..14699)
                     /locus_tag="WGR19_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0798627.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
BASE COUNT         3769 a         4286 c         3237 g         3554 t
ORIGIN      
        1 atatttccct caagcaatgc ggcggcacca atgcctgatt aaaacacaac cggcgttttc
       61 ggcatatatg caaaaacgca ttttccggca cggcgttaca ccgataccga aaaatgcgtc
      121 tgaatgtttc gacaatccga ttaaagcatt atgccaaatc tgcaaaaagt ggcgtagaga
      181 gatagcgttc gccataagaa ggcagcagca cgactatcag cttgccttcg ttttcaggct
      241 gtttggcaag ctgcaacgcg ctccaaaccg ccgcaccgga agaaataccc accaaaatgc
      301 cttctttttc cgctattgcg cgggcggttt caaaagccgc ttcgttcgac actttggtaa
      361 tgctgtcgta aattttggta ttcaaaacgg tcggaataaa gcctgcgccg atgccttgaa
      421 tcgggtgcgg gcctttttcg ccgccgctca atacgggtga agcttcaggc tcgacggcaa
      481 ccactttaac ttcgggtttg tattttttca acacttcgcc cacgccggta atcgtaccgc
      541 ccgtgccgac gccggcaacg aagacatcga ctttgccgtc cgtatcccgc caaatttcct
      601 cggcggttgt tttgcggtgg acttcggggt ttgcctcatt gtcgaactgg cgcggcataa
      661 aataagtgtc gggatgcgcg tccaccaagg atttcgcttt ggcaatcgcg cccgccatac
      721 cttcggcggc aggggtcaga atcagctccg caccaaacgc gcgcaacagc attttgcgct
      781 ccttactcat gctttccggc atggtaatcg ccagcttgta gccgcgtgcg gcacatacca
      841 ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac aatgacggtg tttttgttga
      901 ttttgcccgc tttttcggca ccctcgatca ttgcttcggc aatgcggtct ttgacgctgc
      961 tgcctggatt gaaaaattcc agtttcacgg caacctccgc cttcaaacct tcggtcagac
     1021 ggttcagttt gaccaaaggc gtgttgccga ttagttcggt gatgctgttt gcaattttca
     1081 tcttctgctc ctgaataata aggtttcgac agtaaggcgc aatccccata ggcatcctgt
     1141 ccgattgcag gggatttgcg ccgaaacata aacaaatttc ggatatggct ttatcctaaa
     1201 cctatgccgc ctaaaaaacc aatatccaaa atttctttct ttataacttt ttgttccgat
     1261 acggccgcac cgtattatct tccaatttct gaaagcagca gcaccagtac cgagacctgc
     1321 atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc cgccgaacgt tgacggcggc
     1381 tgccggtcta taccgcagcg gtggacatag gcaaccgcca tcagcacaaa ctgcaacacg
     1441 aaccaataaa ccggcttcag cgcataaagt ttgagcagtg ccgcaaacac aaacacttga
     1501 accgttcccg aaaagcaata atgcaccaac gcacatattc cgacaaaagc aagcgtcatc
     1561 gatacattac tgacggcaaa cagcccgagc agaggatgtt cggggtctgc ctgccatccg
     1621 tttccatccg ttttccggtt ccaatgcccg atgaaaaaaa atatgctgcc caaagtcagg
     1681 taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc cccatatgcc gggcatcagc
     1741 tttgccccca aaaccgatat gcccagccac agcataatac tcgcccaaaa ccattgcaac
     1801 attcctgcgc ggaatatcag aaacagaaaa accgccgcat acagtaaggc agacaaaaaa
     1861 tccaaatgca gcaaaatcat gaatacgcca actcgccttc aaacaccgtt tccgcagggc
     1921 cggtcatcat cacatcgccg ccgcaggccc attcgatata taaagttccg cccggcaaaa
     1981 tcacttctac cgttttccct tcatccaaca accccagacg gatacccgcc accacagccg
     2041 cacacgcgcc cgtaccgcac gcttgggttt cgcccacgcc gcgctcgaac acgcgcaaac
     2101 gaatcgcggt tcggctgaca atctgcataa agccgacgtt gacgcgttcg ggaaactgcc
     2161 tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc gcattctacg tcatcgacca
     2221 caatcaccgc atgggggttg cccatattga cgcagctgac aggctgaatg ccggattcga
     2281 gatgcacccc gtaaatacag gcatcatccc cctcgcccga ttcggggaca aacggtattt
     2341 cagacggcat aaacttcggt ttgcccatat tgaccgtaac cataccgtta tcggacaatt
     2401 tcggaaaaat aacgccattt gccgtttcaa cacaaatttc tttcttatcg gtcaaaccct
     2461 tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc gcattgcccg acctcgctgc
     2521 cgtcggcatt gaaaatacgg taacggaaat ccacgccttc ggtttccgaa cgcccgacca
     2581 ccaaaagctg gtcgaagccc acgccccgga aacggtccgc ccatgccgca atcggcgcgt
     2641 cctcgggggt aaaatcctga ctgaccgcat caatcaccat aaaatcgttg cccaaaccgt
     2701 gcattttggt aaatttcaaa gtgttcatat tgttccgaaa tcccgtatcg tctgttgctt
     2761 ccgaatccga aatcccccgc accgaaggga aatgcttcca tctcccgccg cctgaggatt
     2821 aaaatcagcc attatactgt ttgttacccc ttacaagaaa caccatgttc cgcatattac
     2881 tgttgctttt cccttgtgca atccttgccg cctgcacccg aaacataccg gcaaacccgg
     2941 ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct gtattttgac gcacacggca
     3001 aacccgccga cggcgcagta aagggcggat tttacaaaat tatcgaaata cagggtgcaa
     3061 accgcatcct tattcaagac tttaacagca cgggcaatgc cctcggcgga tcctatgtgc
     3121 cggactgccg caaatcatcc cttttcccga aaaatatttg acttcaaact gcctatgaac
     3181 cacgacatca ctttcctcac cctgttcctg ctcggcttct tcggcggaac gcactgcatc
     3241 ggcatgtgcg gcggattaag cagcgcgttt gcgctccaac tccccccgca tatcaaccgc
     3301 ttttggctga tcctgctgct taacacagga cgggtaagca gctatacggc aatcggcctg
     3361 atactcggat taatcggaca ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat
     3421 attttataca cggccgccaa cctcctgctg ctctttttag gcttatactt gagcggtatt
     3481 tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga tatggcgaaa cctgaacccg
     3541 atactcaacc ggctgttacc cataaaatcc atacccgcct gccttgcggt cggaatatta
     3601 tggggttggc tgccgtgcgg actggtttac agcgcgtcgc tttacgcgct gggaagcggt
     3661 agtgcggcaa cgggcgggtt atatatgctt gcctttgcac tgggtacgct gcccaatctt
     3721 ttggcaatcg gcattttttc cctgcaactg aaaaaaatca tgcaaaaccg atatatccgc
     3781 ctgtgtacgg gattatccgt atcattatgg gcattatgga aacttgccgt cctgtggctg
     3841 taagccccat tcattcccga tacccgtaaa aaataccgtc tgaaacctgt tcagacggca
     3901 ctttcaccct aagcgttact cggcggcatt ttcctcacgg gcaggcgcgt ccagcagggc
     3961 tttgatggac agacggacac ggcctctgtc gtccacttcc aatgctttca cgttcaccac
     4021 ctgaccgact tgcaggtagt cgccgacatt gcgtacgcgc tcgtgggcga tttggctgat
     4081 gtgtaccaaa ccgtctttgc ccggcatcac gctgacaatc gcgccgacat tgttgtcgag
     4141 gattttcacc acagtgcctt cgtacacttt gcccacttcc acttcggcag taatctgctc
     4201 gatgcgtttt ttcgccgcat cgccggcttc ttgagtggtt gcggcaatgg taatcgtacc
     4261 gtcttcggca atattgattt ccgtaccggt ttcagcggta atcgaacgga tggtttcacc
     4321 gcccttaccg ataacttcgc ggattttgtc ttggttgatt ttcatcgtga acaagcgtgg
     4381 cgcgtgtgcg gacagctctt gcgggcccgc aacggcggct ttcatctgat ccaagatgtg
     4441 cagacgcgct tctttggcct gtgccaaagc gatttgcata atttctttgg taatgccttg
     4501 gattttgatg tccatttgca gcgcggtaac gccttcggtc gtaccggcca ctttaaagtc
     4561 catatcgccc aagtggtctt cgtcgcccaa aatgtcggtc aggacggcaa atttgttgcc
     4621 ttccagaatc agacccatcg cgataccggc aacgtgtgct ttcaaaggca cgccggcaga
     4681 cagcaggctc aggcagccgc cgcagacgga agccatagag gaagagccgt tggattcggt
     4741 aatttcggag accacgcgca tggtgtagct gaaatcttca ggtttcggca atacggccaa
     4801 caatgcacgt ttagccaaac ggccgtgacc gatttcacgg cgtttcggtg cgcccatgcg
     4861 gcccacttcg ccggtagagt acggcggaaa gttgtagtgc agcataaagc ggtcggtgta
     4921 ttcgccggac agcgcgtcga tgatttgctc gtcgcgcgaa gtacccaaag ttgcaacggc
     4981 caaagcttgg gtttcgccac gggtaaacaa tgcagaaccg tgcgtgcgcg gcaatacgct
     5041 ggtttggatg ttcagcggac ggacggtgcg ggtgtcgcgg ccgtcgatgc gcggttggcc
     5101 atccaaaatt tggctgcgga cgacatcggc ttccaagtgt ttgaaaatgc ctttgatttc
     5161 gttggctgcc aaagtgtcgg tttcttcggt aatcaaggct tcttttaccg cactccaagc
     5221 ttcgtccaat ttggcagaac gcgcttgttt ttgacggatt ttgaacgctt ctttaatggt
     5281 ttcgccggca atcccgcgga ctttggcaac cagttcctca ttggtttcag gtgctttcca
     5341 atcccaaagt tccggattga cttcgtcggc aaattcattg attgcattga tggcaacctg
     5401 catttgatcg tggccgtaaa ccaccgcgcc cagcatcacg tcttcgggca ggattttggc
     5461 ttcggattcc accatcaaca cggcttttga agtaccggcg accaccaagt ccaattgcga
     5521 tttcgccaat tcggctttag tcggattcaa aacgtacacg ccgtttacat aaccgacgcg
     5581 tgccgcgccg atcgggccgg caaacggtac gccgctcaac accagcgcgg cagatgcacc
     5641 caacattgca ggaatatcag aatcgatttc aggatcgacg gacacgacca tcgctacgat
     5701 ttggatgtcg tggtagaaac cttcagggaa cagcggacga atcggacggt cgatcagacg
     5761 gctggtcagg atttcttttt cgctttgttt gccttcgcgt ttgaagaaac cgccgggaat
     5821 tttgcctgcg gcgtaagtgc gttccaaata atcgacggtc agagggaaga agtcttggcc
     5881 ttctttcact tctttgttgg tggtaacggc aaccaaaacc acggtgtcgc ccatagagac
     5941 tttaacggca gcggcggctt ggcgggcgat ttcgccggtt tccaaagtaa cggtgtggtt
     6001 gccgtattgg aaagttttaa cgtgtttgtc gaacattatt gttcctttca aaataccgca
     6061 ctgctaaaac actaataatg cacactaaaa tccgaatgtg catagttagg gtttcagacc
     6121 gtgcggcagg ttgtaaacaa gctttcagac gacatttcgg acgctgaaag caattgcgga
     6181 ttataaaggc aaccatcctt aaaatccagt attgatacaa taaaaaggtc gtctgaacca
     6241 tatttctttc agacggcctt caaacttaaa aatcaatcct gagtcagcaa agtcaatgct
     6301 tcccgatact tctcgacagt tttctgaatc acatcggcag gcactttcgg cgcaggggct
     6361 tttttgttcc aaccgctttg ttccagccag tcgcggacga attgtttgtc aaaagacggc
     6421 ggattggtgc cgacttcata ttgatccgcc ggccagaagc ggctcgaatc gggagtcaat
     6481 acctcatcca tcagcgtcag cctaccgttc tcatccaaac cgaattcaaa tttggtatcg
     6541 caaatgatga tgccgcgcga tttagcatat tccgccgctt ctgtataaag ctgaatcgct
     6601 ttggcgcgca cttccgccgc caattctttt ccgatactgc gttcgcattc ttcaaagctg
     6661 atgttttcat cgtgatcgcc gactgcggct ttggttgagg gcgtaaaaat cacttcaggc
     6721 agttgttgcg cttcctgcat accttcgggc agccgaatac cgcaaaccga gccggttttt
     6781 tgataatctt tccaaccgct gcctgccaga taaccgcgca caatcgcctc tactttcacc
     6841 ggagtgagct ttttagccac gacggcgcgt ttttctaaaa ctttggcttc gttttcaggc
     6901 aaaacatcgt aaaccgtttg accggtaaag tggttgggca taatatgcgc cagtttttta
     6961 aaccaaaaat tggaaatctg cgtcagaatc tcccctttgc tcggaatcgg gtcgtccaaa
     7021 atcacatcaa acgcggacag gcggtcggaa gcgaccatca gcatacgttt atcgtcgatt
     7081 tcatataaat cgcgcacttt tccagaatag atctttacca aaccaatctc actcatttcg
     7141 cccccctgaa aatatcttga aaataccgac ccgacacccg acaggtttga atcgcaaacc
     7201 gatattctag ccgaagtcgg cgcaaaacaa tacccatggc acaaaaagcc aacccgtcaa
     7261 ccgtcggcaa agttttggca ctataatacc gacagcaagt cctacaatac acttttacca
     7321 aaggaaatac ctcatgagaa tcctattgac aggctcgaaa agccaactgg cacgctgcct
     7381 gcgcgaccgt cttccggaag actgggaaac cattgcgacg gattccgcat ccctagacat
     7441 taccgatgcc gatgccgtct gcaacatggt caaaagtttc caacccgacg ccattgtcaa
     7501 cacggctgcc tatactgccg tcgacaaagc ggaaggcgat gcggcagcgg catttgccgt
     7561 caatgcttcc gccgtttaca accttgcctt ggcagcacat cgcgcccatg cccgattcat
     7621 ccacatctca accgactatg tctttgacgg aaaagggaaa agaccctacc aggaaagcga
     7681 ctttaccaac ccttccaatg tatacggaca atccaaaacc gccggcgaac tgctcgcact
     7741 gtctgccaat cccgacagcc ttatcctgcg gacttcttgg ctgtttagcg aatacgggga
     7801 caactttgtc cgcaccatgc tgaaccttgc gcgggaacgt tccccgctgt ccgccgtcca
     7861 caaccaaatc ggctgcccga cctatgccgg cgacttgtcc gccaccatca tccgcctgtt
     7921 gcagcactcc aatcccgttc gcggcattta ccactacgcc ggcggcaaat ccgtatcttg
     7981 gtacgaattt gtccaacata ttttccaagc ggcatcgcaa cagcagacat ccttccccat
     8041 tcccgaattg actgccgttt cagacaagga atatccgacc gccgccccca ggcccgcata
     8101 cagcattttg gactgccgca aaatcgaaaa cgacttcggc atcaaaccgt cagactggca
     8161 aaaagccctt gcacaggtcg tttccaagct gctctgatgc cgcccgcctc tccgtttccg
     8221 ccgtcaagca ccgccttggc ggttttctta tatagtggat taacaaaaac cagtacggcg
     8281 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
     8341 tgttaatcca ctataaaatc taccgattac acaaacacat atcatcttta cacaaacaca
     8401 tatcatcttt acacaatcat gcttccatca acagtaaaac atgatatgat tgccaacaat
     8461 aacatctcac aataaatttt ctaattttta ttgaaaaaat caataaataa gaattctccc
     8521 aaccgacaaa taaaataaag aaagggttaa tatgcaacat cgaagaagac tagcaattta
     8581 ccaagcatcc aaacgtgctt cctttaccgg caggtcatcc gccccgcaaa aacgtaaaga
     8641 acgttgattt gaaaaaaatg ccgtctgaag tcctgcttca gacggcattt ttacgattcg
     8701 agaacctgcc tcaacttttt ttcatccaaa taccaatggc aaatctcttt atcacctgcc
     8761 aacaaaacag gtaccagctc attatatttc tcttccaaga cgggatcatc atccacatca
     8821 agcacatcca gcccgaaacc gtattcattt tgaaaaggtt tgagtgcatc gcgcattttg
     8881 tggcacaagc tgcaatattc acgaaacatc aaggtcaatt tcatccgcgt ttccttatct
     8941 gtcaatttgc acacgccaaa gccttagacg cagcagaatc atggtctatt tgggaaaaaa
     9001 caatgttttc gaggaagatg atactcaagt tctgccaaaa acagcaaaaa tgccgtctga
     9061 acagttcaga cggcatttcc aaaaccgttt tacgcttgaa cgttgataac cgccgtaacc
     9121 gtcggttgca cattgtgcgc cgtcagaaac gaagcggcaa gctccccaca gcagccgcag
     9181 cagctcgcta cgcccaattg cgactgcaaa tcgcccattg tggtcgcgcc ggcggcgatg
     9241 gtttccttga tttgatggtc ggtaacggca ttgcagatgc agacaaacat tttgtgctcc
     9301 gtgtgttctc aaactatcgt accgatagcg gcttttatta tcgtatgcga atataaataa
     9361 aaacggttcg cattgcaagg tcggtataca cggtttgtct tggtaatttt ttatcaagtt
     9421 tgcattttcg attgatttct tattaaacca aagtagaagc ctcagtttcg ggaatggtgt
     9481 tccttcacta caccctgcaa ccatacgttg gcaacatggg tataaatctg cgtcgtattc
     9541 aaatcggcat gtcccaacat atcctgaacc acgcgcaaat ccaagccgtg ccgcaccaga
     9601 tgcgtggcaa aggcgtggcg caggctgtgc gggctgatgt gcccgatgcc tgcctgactt
     9661 gcatattctt tgacaatcat ccatgccaac tgacgggaaa tgcccgtctt tttctgactg
     9721 acaaacaatg cgtcgcaatt cctgcctttc agcagaagtg ggcgtgcctc cgtataatag
     9781 cgttccaccc aatacgccga ctcctgcccc atcgggacca tcctctgctt atcacccttt
     9841 cccagcgcgg taatacagcc cctgtccaaa tccacattgc cgaagttcag cccgaccgcc
     9901 tcgctgacgc gcaagccggt cgcgtacatc aattcgagca aagccttgtc ccgcaaaccg
     9961 tgcggcgtgt cggtatccgg ggcggcaagc agtcgggaaa tctgctgctc ggtgatcagg
    10021 gtcggaatat tcttgtcgat tttgggcggt ttcagcaaac gggtgggatt gtccgtcctt
    10081 atgccttcac gctccatcca tatatacagg cgtttgcatg ccgataatgc gcgcgcctgc
    10141 gaactccgtt gctctccgtc aacataaacc gccgccgcca aatccgcttc gtccgcatcc
    10201 ttcagcattc tgcccgattg ggacaggcgg cgggcgattt tttccaaatc gcgccggtaa
    10261 ccgtttaaag tattctgact gagccgcctg tccaaccaca gcgtttcaag cagcctgtcg
    10321 atcaaacctt cttccatacc gttccaaaca aatgccgtct gaatcttctt cagacggcat
    10381 ggttcacatt atcgggaaag cgtttccaat acttcctgcg cgtgacccgc cactttgact
    10441 ttccgccatt catgggcgat ttctccatcc ttattcaaga cgaacgtact gcgctcgata
    10501 cctaacgact ctttcccgta cagtttcttc aatttgatga catcaaacag gcggcacact
    10561 gtttcatcct tgtcgctcaa cagctcgaac cggaaaccct gcttggcgca aaaattctga
    10621 tgcgccttta cgccgtcgcg ggaaataccg accacggtat aacccaatgc ctcaaactgt
    10681 tccaaacgcg cattgaaatc caagccttcc gtcgtacagc ccggcgtact gtctttcgga
    10741 taaaaataca cgaccaaagg cagatgttct gccgaatgaa aatccgcacc gctgctcgaa
    10801 ggcagggtaa attcatattt cacatccata gtcctactcc cgatattccc attattcaaa
    10861 acgacacgca gacgcaccgc cgcaattgcc aaaccaaccc cgattctacc gccccaaagg
    10921 acagggattc aaccgccgga aacatccaaa ccgacacacg acggcatgaa aaatatccat
    10981 gtcaaaccac aaaatatgtt ccgatttaaa aacagaatgt tataaaaccc aatccccaaa
    11041 acacaacaag accgcccgcc tacgggcagt ctcctgtcag acgacatact ttacagatgg
    11101 ctgttttttc aacaaaataa cgccaatact caaaaatatg gaatcaaaaa tgtccatcca
    11161 tactctgaaa cgcctgccct catcgctgct gctcggtctc tgcctttccc tgccgtcagc
    11221 ccaccttttt gccgacaacg acattttagg gcaattttta gaacagaaca tgcttacctc
    11281 ctccgatccg atagaaatat tcgccgaaag cacgatacac cccaccaaca cccaagccat
    11341 tacaggcggt ctgattctct cctcacagtc tgccctggtc gtcaacaaca aaaccggaca
    11401 gatactgtat cagaaaaacg ccgacaggat tatgcccatc gcctccattt ccaaactgat
    11461 gagcgcgatg gtcgttttgg atgcaaactt ggacatgaac gaaaccgtta ccattacgcc
    11521 cgacgaaatc gaccgcatca aagggaccgg cagccgtctt gccataggta cggcacttac
    11581 acgcaaagaa ctgctgcacc tgagcctgat gagcagcgaa aaccgcgcca cccatgcatt
    11641 gggcagaacc taccccggcg gcatgggcgc atttgtcgcc gccatgaacc gcaaagccca
    11701 aagcctcggt atgtacggca gccgctttta cgaaccgacc ggactcaact tccaaaacgt
    11761 ttctaccgcc aaagacctga gccttatggt caacgccgcc gcccaatatc cgcaaatccg
    11821 caccaactcg acttccaact acgcctcggt acagaccaaa aacgggcagc agaactacaa
    11881 aaactccaat gccctggtca gagaaggcat gtggaacatc gaattgcaga aaaccggcta
    11941 catacgcgaa gcaggcaggt ctatggttgt caaagccaac attcaaaatc aacccgttac
    12001 catcgtattg ctgaactcgc ccacatccgc cacacgcgtc aacgacgccc gcaaaatcga
    12061 atcgtggatg ctgcagcaac gctcctgaca tacaaatgcc cggcggaaaa ccgcccaaac
    12121 acaaaaaaaa ttggtaattt ttcctattca aggctacaat tcgacacgca cagggcgtct
    12181 gcccgatttc acaatcataa taacggagtt aaaaatatga cagctaaagg acaaatgttg
    12241 caagatccct tcctgaacgc cctgcgtaaa gagcatgttc cggtttcgat ttacttagtt
    12301 aacggtatca aattgcaagg tcaggttgag tctttcgatc aatacgttgt tctcctgaga
    12361 aacacttccg tcacccaaat ggtttacaaa cacgccattt ccaccatcgt accggcacgc
    12421 tccgtcaacc tacaacatga aaacagaccc caagccgcac cgacttcgac cctcgtccaa
    12481 gtggaaaccg tccagcagcc tgccgaataa tccgcacgaa gcatgacgtg tcatatcttt
    12541 caatacctta ccggacaacg gtaaggtatt tttattttca gacagcattt aaaaatgtta
    12601 ttgcaaaaca tccttccatt cgcccattgc cttttgcgga aggcacttcc cgaaggcggc
    12661 aatgctttgg acggcaccgc cggcaacgga cacgacaccc ttttcctcgc acaaaccgca
    12721 ggcatccggg ggaaagtgtg ggcattcgac atccagccgc aagccctgag ccgcactcga
    12781 tgccgtctga aagaagcgag atacgagaat gtgcaactca tcttagacgg acacgaaaac
    12841 ctgaaacaat atgtttcaga accactaaat gccgccattt tcaatttcgg ctggctgccc
    12901 ggcggggaca aaagccttac cacccgcacg gaaaccagca ttgccgccct ttctgccacc
    12961 ttatccctac tgaaagaaaa cggtatgctt attgccgtcc tctatccggg acacgaaaac
    13021 ggcaaacagg aggcagaagc cgtcggacaa tgggcaaaaa acctgcctca agaacagttt
    13081 gccgttttac gttacggctt taccaaccgg aaaaacagcc caccctatct gttggcattt
    13141 gaaaaactgc gtcaaaaata actgtttgcg gtaaaataag ccgattccaa cccttatttt
    13201 gataagagcc gaccatgcag tatctgtttg tcaaatacag ccaccaaatc ttcgttacca
    13261 tcaccatttt ggtattcaac atccgttttt tcctactttg gaaaaatcca gaaaagccct
    13321 tggcgggctt ttggaaggca ctgccacacc ttaacgacac catgctgctg tttacgggat
    13381 tgtggctgat gaaaattacc catttctccc cgttcaacgc gccttggctc ggtacaaaaa
    13441 tcctgcttct gctcgcctat atcgcattgg gtatgatgat gatgcgcgcc cgtccgcgtt
    13501 cgaccaagtt ctacaccgtt tacctgctcg ccatgtgttg cgtcgcctgc atcgtttacc
    13561 ttgccaaaac caaagtcctg cctttctgaa acaccgttat gaacaacaga cattttgccg
    13621 tcatcgccct gggcagtaat cttgaaaacc ctgcccaaca ggtacgcgcc gcattggaca
    13681 cgctgtcgtc ccatcctgac atccgtctta aacaggcttc ctcactgtat atgaccgcgc
    13741 ccgtcggtta cgacaatcag cccgattttg tcaatgccgt ctgcaccgtt tccaccacct
    13801 tggacggcat tgccctgctt gccgaactca accgtatcga agccgatttc ggacgcgaac
    13861 gcagcttccg caacgcgccg cgcacattgg atttggacat tatcgacttt gacggcatct
    13921 ccagcgacga cccccgactc accctgccgc atccgcgcgc gcacgaacgc agtttcgtca
    13981 tacgcccttt ggcagaaatc ctccctgatt ttattttggg aaaacacgga aaggttgccg
    14041 aattgtcaaa acggctgggc aatcaaggca tccgtctttt accggataag taattccgca
    14101 cgcggatgcc gtctgaatgc cggtttcaga cggcattttt cctttgccgc caacacgcgt
    14161 gcaaaaaaat cgccccttgg gaaaggggcg caaaaggaac acaaaccact accaaaactt
    14221 taaatctgca acattgcctg ccgcatactg tatccgacag gatataaagc cctcactaaa
    14281 tcgtttcgag aaatccaaac ttcttcatcg ccgacagaaa atctgccttt ctccggcacc
    14341 agctccaaca aaaacggttg aaccgccgta tgcagcctgt ccttacaccg ccccagcttt
    14401 ctggacaagg cggacagggc gcgtttgtag gcattatcct tgcggtcaag ttcccgcgca
    14461 ctgccgaccc agctcaaacg aaggttgcgg ctttcatcct caatcagcaa aatgcctgtt
    14521 ttgacttccc ccacctctgg ctcgcatgaa agcgaaatat aatatttgcc gtcgatgttg
    14581 acatacgccg cctcatgcac ctgacagttg cagccggaag tggcaagcag cgaataaaca
    14641 gaatcccgat gcagtttcaa aatatcaagg gaaatctgtt tgaaaatact ttccggcatt
    14701 tttccctcct ttcgtctgct gcgttgttta ctggattgac tgcattatac agacatctcc
    14761 cgatcttgca aatgataatt gttcctattt tttaaaaaat ttataaatta ttgatagata
    14821 tgtgaattaa ttttaagatt taaaat
//
LOCUS       DAWFJW010000044        14790 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFJW010000044 DAWFJW010000000
VERSION     DAWFJW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.044"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(90..641)
                     /locus_tag="WGR19_001085"
     CDS             complement(90..641)
                     /locus_tag="WGR19_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798628.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAVKAGG"
     gene            complement(695..1367)
                     /locus_tag="WGR19_001086"
                     /pseudo
     CDS             complement(695..1367)
                     /locus_tag="WGR19_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1429..1539
                     /locus_tag="WGR19_001087"
                     /pseudo
     CDS             1429..1539
                     /locus_tag="WGR19_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            1619..2062
                     /locus_tag="WGR19_001088"
     CDS             1619..2062
                     /locus_tag="WGR19_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0798629.1"
                     /translation="MVFVNPLYEEAEWAGSLFGLHKSFGFLTLTVITLRIVWAVANRA
                     KRPQSDSKAAAAGHGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWM
                     ANLGNTFHGNLGWLLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(2097..2972)
                     /gene="rsmI"
                     /locus_tag="WGR19_001089"
     CDS             complement(2097..2972)
                     /gene="rsmI"
                     /locus_tag="WGR19_001089"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0798630.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGQLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            3018..3365
                     /locus_tag="WGR19_001090"
     CDS             3018..3365
                     /locus_tag="WGR19_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0798631.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            3370..3963
                     /locus_tag="WGR19_001091"
     CDS             3370..3963
                     /locus_tag="WGR19_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0798632.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            4027..4635
                     /locus_tag="WGR19_001092"
     CDS             4027..4635
                     /locus_tag="WGR19_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0798633.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            4643..5293
                     /locus_tag="WGR19_001093"
     CDS             4643..5293
                     /locus_tag="WGR19_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798634.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            5333..6112
                     /gene="map"
                     /locus_tag="WGR19_001094"
     CDS             5333..6112
                     /gene="map"
                     /locus_tag="WGR19_001094"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0798635.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            6267..6566
                     /locus_tag="WGR19_001095"
     CDS             6267..6566
                     /locus_tag="WGR19_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0798636.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            6804..7178
                     /locus_tag="WGR19_001096"
     CDS             6804..7178
                     /locus_tag="WGR19_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0798637.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTDNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(7366..8832)
                     /locus_tag="WGR19_001097"
     CDS             complement(7366..8832)
                     /locus_tag="WGR19_001097"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0798638.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(9369..9959)
                     /locus_tag="WGR19_001098"
     CDS             complement(9369..9959)
                     /locus_tag="WGR19_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0798639.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDSAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            complement(10020..10601)
                     /locus_tag="WGR19_001099"
     CDS             complement(10020..10601)
                     /locus_tag="WGR19_001099"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0798640.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAELYLPYIEPRSRRMWFTPYPADGVKQERKRGR
                     AKLHVPQFAGRKKRVHDLNLLLVPVVGMDRLGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPVEAHDRSLDGFVSEAGILCF"
     gene            10679..10912
                     /locus_tag="WGR19_001100"
     CDS             10679..10912
                     /locus_tag="WGR19_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798641.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            11085..11813
                     /gene="rpsB"
                     /locus_tag="WGR19_001101"
     CDS             11085..11813
                     /gene="rpsB"
                     /locus_tag="WGR19_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0798642.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            11942..12796
                     /locus_tag="WGR19_001102"
     CDS             11942..12796
                     /locus_tag="WGR19_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0798643.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            13040..13759
                     /locus_tag="WGR19_001103"
     CDS             13040..13759
                     /locus_tag="WGR19_001103"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0798644.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            13943..>14790
                     /gene="mafA"
                     /locus_tag="WGR19_001104"
     CDS             13943..>14790
                     /gene="mafA"
                     /locus_tag="WGR19_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0798645.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAVYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIR"
BASE COUNT         3914 a         4333 c         3455 g         3088 t
ORIGIN      
        1 aaagaaaccg ccattagcac tacacccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgccc tatccgcccg ccttgaccgc accccctgcc
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaaccttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaataaggg gctgtcctag ataactagga taaactcgat tttactaatt
      721 gttttaaaat ggaaacaaga acttttatct cactcttgtt aaaacgccat tcgcactcct
      781 ttaaatacag ctcaaaatgc gctttgggaa tgccgttaaa cttgcgtaaa tgacgttttg
      841 cctggttcca aaagttctca attccattaa tatggttttg tcgttcggca aaatgtgtgc
      901 tgtgattgat acgaaaacga agtttcagcg aagctaaaat ggctaaattc gcgcacatct
      961 aatacatcat agctacgata acaatccgta taaacaatgc tgtcaggttt cacttgttca
     1021 cggataatag gaaataaagt agcggtttga gtattcggta cagtaaccgt ataaacctta
     1081 ccatttcgct tcaaaagacc gaatacggcg actttaccgg cagcaccgcg accgcgtttg
     1141 cctttgcgtt gtccgccaaa ataactttca tctgcttcta cttcgccatc aaacatttct
     1201 aaatgtggac tgttttgata aatcagtcat cgtaaacgat gaaaataata ggctgcggta
     1261 tttttattaa cgcctactaa ctctgctgcc gttcttgcag ttgcacctgc gacaaatagc
     1321 tcaatgagtt tattttgttt atactggcct agacgacttt ttctcatagg gataattcta
     1381 acttaatttg aatttcccta gttatctagg acagccccta agttttttat gaacacaaac
     1441 caacctgccg tttacgaccc gttgacacgc gcgctgcact ggctgaccgt tgccggtttc
     1501 atcggcattc tgaccaccat tgtcctgtgg acgatttata gtggattaac aaaaatcagg
     1561 acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact
     1621 ggtttttgtt aatccactat acgaagaggc ggaatgggcg ggcagcctgt tcggcctgca
     1681 caaatctttc ggcttcctca cgctgacggt gattacattg cgcatcgtgt gggcagttgc
     1741 caaccgcgcc aagcgtccgc aaagcgacag caaggctgcg gcggcaggac acggcattct
     1801 gtatctgctc atgcttgccg ttcccgttat cggcatgatc cgccaatacg gcagcggccg
     1861 cggcccgttg aaagtgttcg gcgttgaggt gatgcagggt tcgccggaaa aaatcgagtg
     1921 gatggcaaac ttgggcaaca cgttccacgg caatttgggc tggctgctgt ttgccgccgt
     1981 cgtcggacac gtcgccatgg tcgtcgtcca ccgtgttcaa ggcaaagacg tgctgtaccg
     2041 catgacgggg cgtgtccgtt gattccgttc gcactatggt gccggtccgt ccggcatcat
     2101 ttgtttttcc aagacagtgc cagattgtac aaagcttttt ttccctcgcc cgtgattttg
     2161 gcggcaagct ccgccgcctg tttggtcggc agctcggctg tgaggatttt catgatgttt
     2221 tgcgcggact cggacaagcc ttcgtgtttt tcatcctgcg ccggataaag caccaacacc
     2281 atctcaccgc gcgattggtt gccgtcggca gacaatgccg tctgaatttc cccaaccgtg
     2341 ccgcttaaga acgtttcaaa cgttttcgta atttcgcgcg ccagcattaa tcggcgttcg
     2401 gggaacaatt ccaccatatc ggcaagcgtc gccccgatgc ggtgcggcgt ttcaaacatg
     2461 acgataggaa acgccgcccg cacccatttg gcaaacaatt tcctacgttc gcccgatttc
     2521 ggcggtacaa aaccgttgaa ataaaaatcg gatcccgcca caccagccac actcaaagcc
     2581 gccatcaccg cgcttgcgcc gacaacaggg acaactttaa acccgacctc acgcacgcgg
     2641 cgggcgagtt tcgcgcccgg gtcgcacacg gccggcgtac ccgcatcgga aacctgtgcc
     2701 acaaccatgc cgtctgaaag atagccgaca atcttgtccg ccatctgccg ttcgttgtgt
     2761 tcgcgcacgc tgacgagttg gccctgaatg ccgtacgcgc tcaaaagctg cgcggtaacg
     2821 cgcgtatctt cggcacagat gatgtccgcc ttttgcaata ccgccaaagc gcgcagggta
     2881 atgtccgcca aattgccgat gggcgtggca accacgtata atgtccctcc gacgacgctg
     2941 tcggaggctt tctgcaaatg tttctgaaac ataagaatgc cgtctgaaaa acaaacatta
     3001 taaaggttaa accgattatg cgcctaaacc acaaacaggg cgaggcaggg gaagatgccg
     3061 cgcttgcctt cctccaatcc caaggctgca cactgcttgc ccgcaactgg cactgcgcct
     3121 acggcgaaat cgacctgatt gtcaaaaacg gcggcatgat tctgtttgtt gaagtaaaat
     3181 accgcaaaaa tcggcaattc ggcggtgccg catacagcat ttccccatcc aaattattga
     3241 aactgcaacg aagtgtagag tattatctgc aacagaacag gttgacaaac gtaccgtgcc
     3301 gcctcgacgc ggtactgatt gaaggaaacc gcccgcccga gtggatacag aatattacag
     3361 gttgacgata tgacgacatt acaagaacgc gttgccgccc attttgccga aagcatccgt
     3421 gccaagcagg aagccggaaa agtattggtc gagccgaccg tacaggctgc cgagctgatg
     3481 ctgcaatgcc tgatgaatga cggcaaaatc ctggcctgcg gcaacggcgg ttcggctgcc
     3541 gacgcgcaac acttcgccgc cgaaatgacc ggccgttttg aaaaagaacg catggaactc
     3601 gccgctgtcg cgctgacaac agacacttcc gcgctgacag ccatcggcaa cgactacggt
     3661 ttcgaccacg tattcagcaa acaggtgcgc gcgctcggac gtgcaggcga tgtattggtc
     3721 ggcatttcca cctccggcaa ttccgccaac gtcatcgaag ccgtcaaagc cgcacacgaa
     3781 cgcgatatgc acgtcatcgc cttgaccggc cgcgacggcg gcaaaatcgc cgccatactc
     3841 aaagacaccg acgttttgct caacgttccc catccgcgca ccgcccgtat tcaagaaaac
     3901 cacatcctgc tgatacacgc catgtgcgac tgtatcgact ccgtactgct ggaaggaatg
     3961 taaccctttt cagacggcat ggcgcaaagc aatgccgtct gaaacgccca agaaaggaag
     4021 cacccgatga aacccaaacc gcacaccgtc cgcaccctga ttgccgccat tttcagcctt
     4081 gcccttagcg gctgcgtcag cgcagtaatc ggaagcgccg ccgtcggcgc gaaatccgcc
     4141 gtcgaccgcc gaaccaccgg cgcgcaaacc gacgacaacg ttatggcgtt gcgtatcgaa
     4201 accaccgccc gttcctacct gcaccaaaac aaccaaacca aaggctacac gccccaaatc
     4261 tccgtcgtcg gctacaaccg ccacctgctg ctgctcggac aagtcgccac cgaaggcgaa
     4321 aaacagttcg tcggtcagat tgcacgttcc gaacaggccg ccgaaggcgt gtacaactat
     4381 attaccgtcg cctccctgcc gcgcactgcc ggcgacatcg ccggcgacac ttggaacaca
     4441 tccaaagtcc gcgccacgct gttgggcatc agccccgcca cacaggcgcg cgtcaaaatc
     4501 gttacctacg gcaacgtaac ctacgttatg ggcatcctca cccccgaaga acaggcgcag
     4561 attacccaaa aagtcagcac caccgtcggc gtacaaaaag tcatcaccct ctaccaaaac
     4621 tacgtccaac gctgactcgg caatgccgtc tgaaccgcct tcagacggca ttgcccgaca
     4681 ccccaaaagc acaatcaaaa tggcaaaaaa accgaacaaa cccttcaggc tgacccccaa
     4741 actcctgata cgcgccgtat tgctcatctg tatcaccgcc atcggcgcat tggcaatagg
     4801 catcgtcagc acattcaacc cgaacggcga caaaaccctt caagccgaac cgcaacacac
     4861 cgacagcccc cgcgaaaccg aattctggct gccaaacggc gtagtcggac aagatgccgc
     4921 ccaacccgaa caccaccacg ccgcctcatc cgaacccgca cagccggacg gcacagacga
     4981 aagcggcagc ggactgccgt cccctgccgc acccaagaaa aaccgggtca aaccgcaacc
     5041 tgccgacaca gctcaaaccg acaggcagcc ggacgacgcc ggaacacaag ctgaaaacac
     5101 actcaaagaa acccccgtac tgcccacaaa cgtcccccgt cccgaacccc gaaaagaaac
     5161 acccgaaaaa caggcgcagc ccaaagaaac acccaaagaa aaagaaacgc ccaaagaaaa
     5221 ccataccaaa ccggacaccc cgaaaaacac gccgcccaaa ccccataaag aaattctcga
     5281 caacctcttc tgacccggca cggcaggcac acccgcaatc caaggaagca ttatgaacgg
     5341 catcatcatc aaaacccccg aagaaatcga aaaaatgcgc gagctgggca aactcgtcgc
     5401 cgaagccctc gactacatcg gacaattcgt caaacccggc gtaaccaccg acgaaatcga
     5461 caaactcgtt tacgactacc acgtcaacgt ccaaggcggc tatcccgccc ccctgcacta
     5521 cggcaacccg ccctacccca aatcctgctg cacctccgtc aaccacgtca tctgccacgg
     5581 cattcccgac gacaagccgc tcaaagaagg cgacattatc aacatcgacc tcaccatcaa
     5641 aaaagacggc ttccacggcg actccagccg tatgtttacc gtcggcaaag tctcccccat
     5701 cgcccaacgc ctgatcgacg taacccacgc ctccatgatg gcgggcatag aagccgtcaa
     5761 acccggcgcg acactgggcg acgtaggtta cgcctgccaa caggttgccg aaaacgccgg
     5821 ctattccgtc gtacaggaat tctgcggaca cggcatcggg cgcggtttcc acgaagcccc
     5881 gcaagtgttg cactacggaa aaaaaggaca gggccccgtt ctaaaaccgg gtatgatttt
     5941 taccgtcgaa ccgatgatca accaaggcaa acgccacctg cgtatcctca acgacggctg
     6001 gacggtggtt accaaagacc gctccctctc cgcccaatgg gaacacgaag tcttggtgac
     6061 cgaaaccggc tacgaaatcc tcaccgtcag ccccgcctcc ggcaaaccct gaaaccggac
     6121 gtatccgccc cataaaaaca aacaatgccg tctgaaagaa acggcagata tgatatataa
     6181 tataaaaaca ggcttgaccc ggcacattac gaaaacaaag caaatcggaa tttgccccgc
     6241 aaccagacaa acttaaagga agttttatga aaatatttga aaatatagaa gatgttaaag
     6301 ccatccgtaa aaagaccggg ctgaaccaga tagacttctg gggcaaggtc ggcgttaccc
     6361 agtccggagg atcgcgctac gaaaccggcc gcaaaatgcc caaacccgta cgcgaactgc
     6421 tccgcctcgt ccatatcgaa tgcatcgatt tggcgaaagt caacaaaaaa gatatggaaa
     6481 tcgccgccct gttgaaaaaa caccatcccg acctgtatgc cgagttgtcc aaacagacca
     6541 agtccgaaag aaaaaaacaa agttaaaccg caacctccgg atgcccgaca gtttttcatt
     6601 tccgaaaaac gcaaacaatg ccgtctgaaa caccggacag gccgccgtat cccgcctgcc
     6661 gcccctgcct caaaccgccg aaccgcccga acccgccttt ttacaaactt tatccaattt
     6721 cctgtttatt tcgggatacg ccgacattag aatgtcaaac agctcgaaat gggcaaactc
     6781 cacatccatc caaaggaata aaaatgaaac ttctgaccac cgcaatcctg tcttccgcaa
     6841 tcgcgctcag cagtatggct gccgccgctg gcacggacaa ccccactgtt gcaaaaaaaa
     6901 ccgtcagcta cgtctgccag caaggtaaaa aagtcaaagt aacctacggc ttcaacaaac
     6961 agggtctgac cacatacgct tccgccgtca tcaatggcaa acgcgtgcaa atgcctgtca
     7021 atttggacaa atccgacaat gtggaaacat tctacggcaa agaaggcggt tatgttttgg
     7081 gtaccggcgt gatggatggc aaatcctacc gcaaacagcc cattatgatt accgcacctg
     7141 acaaccaaat cgtcttcaaa gactgttccc cacgttaatc aggcaacaaa aaacagcgtt
     7201 ttcagaaatg aaaacgctgt ttttttgacc gttccattat tcacaaaagg gaaaaaacga
     7261 ttacctgccc cgtgtatcaa aacctgccct gccggatgaa gggcataacc ggcagggacg
     7321 gcgtcaacac catatggggg tacggctttt cttgaaagat tcggcttaaa tatccaatac
     7381 tttcgcggta taggcgataa tttcatccgc cctttcaggg ttttcgttca acttgatgcc
     7441 gtaacccggt accagctctt tcagacggtc ttcccaagac ggggcgcgct cggggaagca
     7501 ttggtgcatc agccggatca tcagcggcac agcggtcgat gcgcccggcg acgcgcccag
     7561 caatgcggcg agtgagccgt cggcgtgggc gacaatctcc gtaccaaact ggagcacgcc
     7621 gcctttttcg gagtcttttt taatgatttg gacgcgttgc cctgcggtga tgagttccca
     7681 gtcgtcgggg tttgcctcgg ggtagtattc cagcagggag gcgaagcgtt cttctttggt
     7741 tttacgcaat tcgcccagca ggtatttggt cagcggcata ttcgcccagc cggcgcacag
     7801 cataggatag aggttgtcca tatggatgga cagcggcaaa tccataagcg agccttgctt
     7861 gaggaagttg gaacggaagc ctgcgtaagg gccgaacata aggtggcgtt tgccgtccac
     7921 gttgcgtgtg tcgaggtgcg ggacggacat cggcggcgcg ccgacggaag cctgcccgta
     7981 cactttggcg ttgtgttgtt cggcggtttc ggggttgctg ttgcggaaga acaggccgga
     8041 cacggggaag ccgccgtagc ctttgccttc ggggataccg gatttctgca gcagggtcag
     8101 cgcgccgccg cccgcgccga ggaagaggaa gcgggtgcgg agggtgagct gcccgtcggg
     8161 gttgcgggta tcggcggttt tgagcaccca cgcgccgtcg gattcgcgtt tgatgtcttc
     8221 gacgtggcgg ttgaactcgg tttttacgcc cttgccctgc aaatatttca ccatttggcg
     8281 cgtcagccgt ccgaaatcga catccgtacc ttcggcggag tagttggcgg cgacgggttg
     8341 gttttcgtcc cggccgcgca tcatcagcgg agcccaatcg gaaattttgt tccgatcggt
     8401 ggaaaattcc atattttcaa aaagtttttg ggttttaaac gcgtcataac gtttttgaag
     8461 ataagaacaa tggtcttcat tcatcaccaa agacatatgc ggcacggcat tgatgaagga
     8521 attgtcttcc aacttgcctt ccgcgaccag cgtcgcccaa aactggcggc tgacatgaaa
     8581 ctgttcggca atattgaggg cgcgcgccgg atcgataatc ccatttgcac ccaacggcgc
     8641 atagttcaat tcgcacagcg cggaatgccc cgtgccggcg ttgttccacg cgtttgacga
     8701 ttccaacgcc acatcttcca agcgttcaat cagggtgatt tcccaagacg gttcgagttc
     8761 tttgagcaaa acgcccaaag tcgcgctcat aatgccgccg cccaccaaga caacgtctgt
     8821 cgcttcagcc atggtttact cctaaaaaac aggcatcttc tgcccttatg gttatttgcc
     8881 gtactacaaa cgcctgaatc gcaaaagcag ggaaaaccgg caatggtgtg tgtccgagta
     8941 tgctgtttcg gggttggaat gcgttgcaag catggcttcc gacaccgctt caggggcttg
     9001 taatatgtta tcgtgaatgt agtggatttt actgggaaat gcaaagtttt tctgtcgccc
     9061 gccaagtcgg gaaactgcga aatgaaaaat aaaaatagtt atttatctat atatatcaaa
     9121 tttttaatag ataaaaaatc aaaattgttt atatattaat ttttaaaaga ttgtcagcat
     9181 attgcgttaa gttttttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     9241 acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
     9301 ctttgagcta aggcgaggca acgctgtact ggtttttgtt aatccactat aaatttgaaa
     9361 atactgcctc acacctgcac gccataccct gccaacctgc cggtcaggat ttccctgttt
     9421 ttgcaccaat cttccctcag catactgtac acgaccgtat cgcgcacact gccgtcttta
     9481 cggagcatat gcatacgcag cacgccgtct ttttccgcac ccagccgttc gatggcacgt
     9541 tgcgaggcaa ggttcagaat atccgtgcgc catcccacgc aacggcaagc caaaacatca
     9601 aatgcggaat ccaacagcat gattttgcaa caggtgttta tccgtgtccg ccgtgccgat
     9661 gccgcatacc atgtgaatcc gatatccaaa cgcggaatct gcggttcaaa atgataatac
     9721 gccgttgtcc cgaccaccct gcccgcctct tcatcgacaa ccgcaaacgc caaacgcgtt
     9781 gccaatgctg tcccgatata gtctgccacc ctatccggat ggggcgcgga cgttaccccc
     9841 agcttccaaa cctccccatc gcaaaccgcc tcgcgcaaac ccgtttcatg atgcacatcc
     9901 aacggttcga gacgaacgcc gcccaacgac aagaccggca gtattatctt ttccgacatc
     9961 cttttctccc aatattccgc cttcagacgg catttccgcc cggaatgccg tctgaacggc
    10021 taaaaacaca atatccccgc ctccgacaca aaaccgtcca aagaccggtc gtgcgcctcg
    10081 accggcagcc tgtccaccaa ctggcaggca aagcccacgc ccacggtttt tgcctgcaaa
    10141 cggtatttca tcgctgaaag cgtcgcatcg taatagccgc ctgcctgtcc caagcggtag
    10201 cccagcctgt ccataccgac cactggcaca agcaggaggt tcaaatcatg cacacgcttt
    10261 ttccgacctg caaactgagg gacatgcagc ttcgccctac cgcgcttgcg ttcttgtttt
    10321 actccatcgg caggatacgg cgtaaaccac atccgccgcg aacgcggttc gatataaggc
    10381 aggtagagtt ccgcaccgcg tttttgcgcc gcgcggacaa agccgtccaa acgcaattcc
    10441 ttgcccatcg gccaatacac gccgattttc cgcccttttt taatataacg tttgagcagg
    10501 tggttgattt ttaccgttgc cgccgcccgc acgtcccgcc ccatttgcga acgccgcccg
    10561 cgcaattcgc ggcgcagggc gcgtttttcc tcgttcctca tttcagacgg cctttcagga
    10621 ttgcggtaga atgttgcgat tataacgatt ttgttaacat tcaaacagga cgcacacaat
    10681 gtggcacatc gtcgccatcg gctatctttt tgttgccgtt atgtattccg ccgcgcagcc
    10741 gagtattgcg cgcgccttga tttatttggt tttttgggcg gtgctgccca ccgtgttcac
    10801 ggttttcacc attaccgtcc gccgccgcaa ccacctgatg aggcagcagg aacaggcgga
    10861 atccgaacag cagcgcgcac aacggcaaaa agacagcggc acaaaaccct gaatcccttt
    10921 tcagacggca tcttatccgc tataatccgt cagttttcca tttcggaaac acactatttt
    10981 ttaaaactta tgcccacttt cgccgaaggg tgcttgacaa taggcgtgac ctatcaagtt
    11041 ctatgcgatt gaatgtgtgc tcttaaccct ttcaaggaaa taaaatgtct caaattacta
    11101 tgcgtcagat gattgaagcc ggtgttcact tcggccacca aacccgtttc tggaacccga
    11161 aaatggcaca atacattttc ggtgcgcgca acaaaatcca tatcgtaaac ttggaaaaaa
    11221 ccctgccgat gttccaagac gcgcaagaag ccgtacgccg tttggttgcc aacaaaggta
    11281 cagtattgtt cgtaggtacc aaacgccaag cccgcgacat catccgcgaa gaagcgaccc
    11341 gtgccggtat gcctttcgtt gattaccgct ggttgggcgg tatgctgacc aactacaaaa
    11401 ccgttaagca atccatcaaa cgcctggaag aaaaaaccgc agccttggaa aatgctgccg
    11461 aaagcggttt cagcaaaaaa gaaattctgg aaatgcaacg cgatgttgaa aaactggaac
    11521 gttctttggg cggtatcaaa aacatgaaag gcctgcctga cgcgattttc gttatcgata
    11581 ccggctacca aaaaggtact ctggttgaag ctgaaaaatt gggcatccct gttatcgccg
    11641 tagtcgatac caacaacagc cccgacggcg tgaaatacgt tatccccggc aacgacgact
    11701 ccgccaaagc catccgcctg tactgccgcg gcatcgctga cgcagttttg gaaggcaaaa
    11761 accaagcgct gcaagaaacc gtagccgctg cccaagaagc cgctgccgag taatccggca
    11821 aaccgaagag gggcgttatg ccccttttct caaatatgcc gtctgaacgt ccgttcgcgg
    11881 cacacgattc ccgaatgcgg aaaatccttt ccgtatttcc caaaaatcta ggagattcaa
    11941 aatggcagaa attactgcaa aaatggttgc cgatctgcgc gccgctaccg gcctgggcat
    12001 gatggaatgc aaaaaagcct tggttgaagc cgaaggcaac ttcgacaaag ccgaagaaat
    12061 cctgcgtatc aaatccggtg cgaaagccgg taaactggcc ggccgtaccg ctgccgaagg
    12121 cgtattggct tacgcgatca acggcaatgt cggcgcattg gtcgaagtaa actgcgaaac
    12181 cgacttcgtt gcgaaagacg cgggcttcgt agaatttgcc aacttcgttg cgaaaaccgc
    12241 cgccgagaaa aaaccggctt ctgttgaaga actgagcgaa ctggttgaag cagaacgcaa
    12301 agccatcatc gccaaattgg gcgagaacat gtctgtccgt cgcttccaag tgatcgacac
    12361 tgccaaccaa ctggttgcct acatccacgg cgcattggcg accgaaggcg tattggttga
    12421 gtacaaaggc tctgaagacg tagcacgcaa aatcggtatg cacattgttg ccgctaaacc
    12481 acaatgcgta agcgaagccg aagtagatgc cgaaaccgtt gaaaaagaac gccacatcta
    12541 caccgaacaa gccatcgctt ccggcaaacc tgccgacatc gccgctaaaa tggttgaagg
    12601 ccgtattcgc aaattcttgg ctgaaatcac tctgaacggc caagcattcg tgatgaaccc
    12661 cgatcaaact gttgcccaat tcgccaaaga aaacggcact gaagtggtca gcttcgtccg
    12721 ctacaaagta ggcgacggta tcgagaaagc cgttgtcgac tacgcagccg aagtagccgc
    12781 agccgccaaa gtgtaaagca cttatgaaaa agaaagcacc tggattccaa acgaatcagg
    12841 gtgctttttt ttgggaaagg cccatatttt ccggcggcag ggttcaaatg cccgccccgc
    12901 ccgaaaacag cggcggcaaa ttcagcagcc cgttttcaca ccgccggcac gccgcatacg
    12961 ccctgccaag gcatacacct ttcagacggc atcccgcccg gaagccggca acatccccaa
    13021 cgagaaagca gaatatccca tgacacagca aatcaaatac aaacgcgtat tactgaaact
    13081 ctccggcgaa tccctgatgg gttccgatcc gttcggcatc aatcacgata ccatcgttca
    13141 aactgtcggc gaaattgccg aagtcgttaa aatgggcgtg caggtcggta tcgttgtcgg
    13201 cggcggtaac attttccgcg gcgtatctgc acaagcaggc agcatggacc gcgccactgc
    13261 cgactacatg ggcatgatgg cgaccgtgat gaacgccttg gcactcaaag acgcatttga
    13321 aactttaggc atcaaagcgc gcgtacaatc cgcactgtct atgcagcaaa tcgctgaaac
    13381 ttacgcccgt cctaaagcca tccaatattt ggaagaaggc aaagtcgtga tttttgccgc
    13441 cggtaccggt aacccgttct tcacgaccga cactgccgcc gcattgcgcg gtgcggaaat
    13501 gaactgcgac gtgatgctca aagccaccaa cgtcgacggt gtatacaccg cagacccgaa
    13561 aaaagacccg tccgccacac gctacgaaac cattactttt gacgaagcct tgttgaaaaa
    13621 cctcaaagtc atggacgcga ccgctttcgc cctctgccgc gaacgcaagc tcaatatcgt
    13681 cgtcttcggc atcgccaaag aaggctcgct caaacgcgtc attaccggcg aagacgaggg
    13741 aacgctggtt cactgctgat tgaccatagt gtcggcagat atagtcgcat atgggcttca
    13801 gacagccatt tattatatgg agattatagt ggattaaatt taaaccagta cggcgttgcc
    13861 tcgccttgcc gtactggttt aaatttaatc cactatattt acaatcttga tacaatttgt
    13921 ctttcatcaa aggagaaaat ctatgcaagc acggctgctg atacctattc ttttttcagt
    13981 ttttatttta tccgcctgcg ggacactgac aggtattcca tcgcatggcg gaggtaaacg
    14041 ctttgcggtc gaacaagaac ttgtggccgc ttctgccaga gctgccgtta aagacatgga
    14101 tttacaggca ttacacggac gaaaagttgc attgtacatt gccactatgg gcgaccaagg
    14161 ttcaggcagt ttgacaggag gtcgctactc cattgatgca ctgattcgtg gcgaatacat
    14221 aaacagccct gccgtccgta ccgattacac ctatccacgt tacgaaacca ccgctgaaac
    14281 aacatcaggc ggtttgacag gtttaaccac ttctttatct acacttaatg cccctgcact
    14341 ctcgcgcacc caatcagacg gtagcggaag taggagcagt ctgggcttaa atattggcgg
    14401 gatgggggat tatcgaaatg aaaccttgac gactaaccct cgcgacactg cctttctttc
    14461 ccacttggtg caaaccgtat ttttcctgcg cggcatagac gttgtttctc ctgccaatgc
    14521 cgatacagat gtgtttatta acatcgacgt atttggaacg atacgcaaca gaaccgaaat
    14581 gcacctatac aatgccgaaa cactgaaagc ccaaacaaaa ctggaatatt tcgcagtaga
    14641 cagaaccaat aaaaaattgc tcatcaaacc aaaaaccaat gcgtttgaag ctgtctataa
    14701 agaaaattac gcattgtgga tggggccgta taaagtaagc aaaggaatta aaccgacgga
    14761 aggattaatg gtcgatttct ccgatatccg
//
LOCUS       DAWFJW010000045        14689 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFJW010000045 DAWFJW010000000
VERSION     DAWFJW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.045"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(338..4507)
                     /locus_tag="WGR19_001105"
     CDS             complement(338..4507)
                     /locus_tag="WGR19_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0798646.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTGKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6409)
                     /locus_tag="WGR19_001106"
     CDS             complement(4562..6409)
                     /locus_tag="WGR19_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0798647.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6974..8203
                     /gene="sstT"
                     /locus_tag="WGR19_001107"
     CDS             6974..8203
                     /gene="sstT"
                     /locus_tag="WGR19_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0798648.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8315..9598
                     /locus_tag="WGR19_001108"
     CDS             8315..9598
                     /locus_tag="WGR19_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0798649.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPS
                     PSGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGTWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9673..9888)
                     /locus_tag="WGR19_001109"
     CDS             complement(9673..9888)
                     /locus_tag="WGR19_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798650.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLKAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9881..10097)
                     /locus_tag="WGR19_001110"
                     /pseudo
     CDS             complement(9881..10097)
                     /locus_tag="WGR19_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            complement(10304..11647)
                     /gene="argG"
                     /locus_tag="WGR19_001111"
     CDS             complement(10304..11647)
                     /gene="argG"
                     /locus_tag="WGR19_001111"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0798651.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKQ"
     gene            12110..12910
                     /locus_tag="WGR19_001112"
     CDS             12110..12910
                     /locus_tag="WGR19_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0798652.1"
                     /translation="MNAFNLIIIGDEILHGSSQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHSEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLEGLGAELKNRVN"
     gene            12950..13936
                     /locus_tag="WGR19_001113"
     CDS             12950..13936
                     /locus_tag="WGR19_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0798653.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14593..14689
                     /locus_tag="WGR19_001114"
                     /pseudo
     CDS             14593..14689
                     /locus_tag="WGR19_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3109 a         3752 c         4374 g         3454 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacagcgttg
      121 ccttgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccca aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc tacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg cgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttggcgatg cccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt gcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttgatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc gccgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatatt gaggttaagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ctcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggatcgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct tttccggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcggcggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agctgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaaaccg gctttggtgt tttccaaatc gagcgagcct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 aaggtacgaa ggcggccgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggcc gcccccgacg aactgctcca cggtgtgtcg gcggccttca
     3901 ggtcaaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtgccggcg agccagccga ggaaacatac tgccaaaatc agaacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtagggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatagtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaaacg cgttcccagg ctgatgtgcc agcggatttt cttatcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcaagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcgtcgcc catatcgtgg aacaccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gtccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgtcggca ttatcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagtttcttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgtgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc tgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgagc
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggagac ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agtgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaggccgg agacgatttg ttcggggtaa cgctgtgtgc
     5701 cggtgatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cgacggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg gcaagcgggt
     5821 agcctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgt accgtataag ctccgtcttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg cggagcatgg ttttaacgtt gtccggtgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactat cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt aactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttgatcatca tggtacgggt gtcgtgcatc ggtgtggcag
     6421 atttcgattc taacactgtt cggacggggt ttcgaacggc tgtttttgtg ttgttcggac
     6481 ggggcgggac gcttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttctaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgtcggaa aataaacggg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgtcg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttca tagtggatta acaaaaacca gtacggcgtt ggctcgcctt agctcaaaga
     6781 gaacgattct ttaaggtgct gaagcaccaa gtgaatcggt tctgtactat ttgtactgtc
     6841 tgcggcttcg tcgccttgtc atgatttttg ttaatccact ataaaacaca gccgaaaaat
     6901 aaaaaaaaca cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac
     6961 ataaggaaga attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct
     7021 ggtcaggcag attgccgccg gtttggcgtt gggcatcgta atcggttcgg tttccccgca
     7081 actgggcttg gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc
     7141 gccggttttg gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa
     7201 ggcgcatatc aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac
     7261 cgccgtcatc gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt
     7321 gtccgccgcg ccgccttccg gcattgtgga agtattgaaa tcgctgctga tgaacctggt
     7381 cgccaacccg attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt
     7441 ggttttgggc gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga
     7501 tttggcggaa gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat
     7561 tttcgggctg gtgtcgtcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc
     7621 gaagctgctt gcggtactgt tgggctgtat ggcgtttatc gcgctggtgg tcaatcccgc
     7681 catcgtgtgg tggaaaatcc gccgcaaccc ttatccgctg gtgtttacct gcctgcgcga
     7741 aagcggcgtg tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc
     7801 tttggcgaaa aaactggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc
     7861 gactgtcaat atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgca
     7921 cacccaaggc atacaggttg attttgccac cgcgctgctg ttgagcctgg tggcgacggt
     7981 cagcgcgtgc ggcgcgtccg gcgtggcggg cggttcgctg ctgctgattc cgctggcgtg
     8041 cagcctgttc ggcatcagca acgatgtcgc catgcaggtg gttgccgtcg gctttatcat
     8101 cggcgtgatt caggattcgg cggaaacggc gttgaattct tcaaccgatg ttttgtttac
     8161 cgctgccgcc gatttgggcc gacagagaaa ccgggcggaa taaaccaagc tatgccgtct
     8221 gaagcccgtt tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac
     8281 aataccggct gccttgaata tgaaggggat gatgatgttt gaacgcagtg tgattgcaat
     8341 ggcttgtatt tttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa
     8401 atcggcggac acgctgtcaa aaccggccgc tcctgttgtt gctgaaaaag agacagaggt
     8461 aaaagaagat gcgccacagg caggttctca aggacagggc gcgccatcca cacaaggcag
     8521 ccaagatatg gcggcagttt cggcagaaaa tacaggcaat ggcggtgcgg caacaacgga
     8581 caaacccaaa aatgaagacg agggaccgca aaatgatatg ccgcaaaatt ccgccgaatc
     8641 cgcaaatcaa acagggaaca accaacccgc cgattcttca gattccgccc ccgcgtcaaa
     8701 ccctgcacct gcgaatggcg gtagcaattt tggaagggtt gatttggcta atggcgtttt
     8761 gattgatggg ccgtcgcaaa atataacgtt gacccactgt aaaggcgatt cttgtaatgg
     8821 tgataattta ttggatgaag aagcaccgtc aaaatcagaa tttgaaaatt taaatgagtc
     8881 tgaacgaatt gagaaatata agaaagatgg gaaaagcgat aaatttacta atttggttgc
     8941 gacagcagtt caagctaatg gaactaacaa atatgtcatc atttataaag acaagtccgc
     9001 ttcatcttca tctgcgcgat tcaggcgttc tgcacggtcg aggaggtcgc ttcctgccga
     9061 gatgccgcta atccccgtca atcaggcgga tacgctgatt gtcgatgggg aagcggtcag
     9121 cctgacgggg cattccggca atatcttcgc gcccgaaggg aattaccggt atctgactta
     9181 cggggcggaa aaattgcccg gcggatcgta tgccctccgt gtgcaaggcg aaccggcaaa
     9241 aggcgaaatg cttgctggca cggccgtgta caacggcgaa gtgctgcatt ttcatacgga
     9301 aaacggccgt ccgtccccgt ccggaggcag gtttgccgca aaagtcgatt tcggcagcaa
     9361 atctgtggac ggcattatcg acagcggcga tgatttgcat atgggtacgc aaaaattcaa
     9421 agccgttatc gatggaaacg gctttaaggg gacttggacg gaaaatggcg gcggggatgt
     9481 ttccggaagg ttttacggcc cggccggcga agaagtggcg ggaaaataca gctatcgccc
     9541 gacagatgcg gaaaagggcg gattcggcgt gtttgccggc aaaaaagagc aggattgatg
     9601 ctgatgccgc ctgaagcggg ggcgttcaga cggcattgtg atgtttgggg tggggtccgg
     9661 ttcaagtccg catcagttga taaacccgtc gtctttcatt ttcttttcgg aaaaatactg
     9721 gccgaatacc agcagggcag cgaggatgat gacaaacagt cccgccatag tcagagtgat
     9781 tttgaaaaac aattccgctt tgagaaaaga aaaccagagt tgcacaatca gcagcagcac
     9841 ccagccggca aacagtccga gacaggcata aacgccgagc ttaaacacga tcccgctcca
     9901 aaatgacgac gtaggcctcg cgtttgaaaa acagcagcat acggcgcaaa tctttttcca
     9961 tcgttacgac ccgccagcct tcgggggatt tgtttgttga ggaacgcgct gaaattgacg
    10021 gggttgacct ttgccgcgcc gaaaaacagg ctggacaaca ggctttcctg ataaatgatg
    10081 actttgtatt ctttcatcgt atttcctttt cggtttaaac cccgcccttt aaggcggtag
    10141 aatcagactt tatttgggag gggcgcaacc ccttccaaat caggacgaca catagggcgg
    10201 tgctttatgt gccgccctgt gtgttgaaac ataggattcc tttaattgag ggattgcctt
    10261 ttcagacaat cccttaaatt gcagcgatgc aatgcggtga aacttattgc ttattaccca
    10321 actgcggcaa taccgaacct tcgcccaatg ccaacaaacc gctttgcgag taaatgccca
    10381 gtttggcgcg ggtgtcggta atgtcgaggt tgcgcatagt cagttgtccg atacggtcga
    10441 gcggtgtgaa cgctgcgtct tcgacttttt ccatactcag gcgttcgggt tggtaggtca
    10501 ggttgggcga ttcggtgttc agaatcgagt agtcgttgcc gcgacgcaat tcgagggtta
    10561 cttcgccggt aatggctttg gcaacccagc gttgggcggt ttcgcgcaac atcaaggctt
    10621 ggctgtcgaa ccagcggcct tggtagagca ggcggccgag gcgcaggccg ttgatgcggt
    10681 attgttcgat ggtgtcttcg ttgtggatac cggtcaccaa acgctcgtag gcgatgtgga
    10741 acaacgccat tcccggggct tcgtagatgc cgcgcgattt ggcttcgatg atgcggtttt
    10801 cgatttggtc gctcataccc agaccgtggc ggccgccgat gcggttggct tcgaggaaga
    10861 gttcgacggg gtcggcgtat tctttgccgt tcagtgcaac cggcacgcct tcttcaaagc
    10921 gcacgctgac ttcttcgggg ctgacttcaa cgttttcgtc ccaaaaggca acgcccataa
    10981 tgggtttgac gattttgatg cccgagttca aaaattccaa gtctttggct tcgtgggtcg
    11041 cgccgagcat attggaatcg gtggagtagg ctttttcaac cgacattttg tagttgaagc
    11101 cgttggcaat cagaaattcg ctcatttcgt gacggccgcc gagttcgtcg ataaattgct
    11161 ggtcgagcca gggtttgtag attttcagcg cgggattggt gagcaaaccg tagcggtaga
    11221 aacgctcgat gtcgttgcct ttgtaggtgc tgccgtcgcc ccagatgttt acgtcgtctt
    11281 ctttcattgc ggaaacaagc atcgtgccgg ttacggcgcg gcccagaggc gtggtgttga
    11341 aataggcgat gccgccggtg gaaacgtgga acgcgccgca ttggatggcg gcgatgcctt
    11401 cgtgtgccaa ctgcgcgcgg cagtcgatta agcgggcgtt ttccgcaccg tattccatcg
    11461 cttttttggg aatggcgttg tagtcgtctt catcgggctg accgaggttg gcagtgtagg
    11521 cataaggcag tgcgcctttg agtttcatcc acaacagcgc ggcggaggta tcaagtccgc
    11581 cggagaaggc gatgccgact ttctgaccga cggggaggtt ttgtaaaata gtatggtttt
    11641 ggctcataag ggtatccttc ggacttggaa aaaggagttc gtcgtgtttc cttttcgatt
    11701 gaaaccccgc cacttggaca tctgtccttc ggggcggtag aatcagactt tatttgggag
    11761 gggcgtaatc ccttccaaat caggacggca catagggcgg tgctttatgt gtcgtcccgt
    11821 gtgttgaaac attatcttat aaatataacc cattgtcagc cgttaaggct gtctgaaaag
    11881 aagctttgcc cgtttcgctg aaaatctttt cagacggtct ttacgccatc ctcaacctga
    11941 ctccctcccc tgcgggggag ggttggggag agggtgcaga cagcagccct taagcaaacg
    12001 ctgcctgaaa aacgcaagct cgaccctctc cctaaccctc tcccatggga gagggaacaa
    12061 atcacagcac catcgacatt ttcagacggc atcggtaaag aaaccatcta tgaacgcgtt
    12121 caacctcatc atcatcggcg acgaaatctt acacggcagc agccaagaca agcatttcgc
    12181 ctttttcaaa tccctgctcg aatccaaagg gctgaagctc aatcaggtgc aatacctgcc
    12241 cgacgaaccc gatttgctgg tcaggcaact gcgccgcagc ttttcagacg gcatgccaac
    12301 tttcgttacc ggcggtatcg gcgccacgcc cgacgaccac acccgccaag ccgcagccgc
    12361 tgctttggat ttgcccgtcg tccgccattc tgaagccgcc aagtttatcg aaggcataac
    12421 ccagaaacgc ggcgagccgc ttgattcgcc ggaacacgcc caacgcctga aaatggcaga
    12481 ttttcccgaa ggcgcggaat tggtgcccaa tccgtttaac aacatcgccg gattttctat
    12541 ccgcgagcat tatttcttcc ccggctttcc tgtgatggcg cacccgatgg cggaatgggt
    12601 attggaaact tattacgccg accgcttcaa ccaaaccgaa cgcggcagcc gcagcgtgta
    12661 tgtgtttgag cagcccgaat cgcgcattac gccgcttatg gagcatatcg agcaaactta
    12721 tcccggcgtg cgttcgtaca gcctgcccag cgtcggttgg acgcattcag acggcacgca
    12781 ggtcaaaccg cacattgagt tcggcatcaa agccgaagga gaagccgtca accttttgga
    12841 cgcggcgtgg gcggaagtgt tgcacagctt ggaaggattg ggagcagagc tgaaaaatcg
    12901 ggtaaactga aaaacgtttg cccaaatcgg acgatattta aggaaaacca tgcaataccg
    12961 aatcagaaga gaaaacgaag cacccgaagc aaaaaatacg ggggaaacct tatgggagcg
    13021 cgacatcatg cgcgaagtgc tgctgtccgc ctatcaggac aggcgcaggg agcggatgtg
    13081 gaaaaacatc tggcgtgccg tcagcaccct gattctggtt gccctgattg ccggtatttt
    13141 ccgaaaagac gaagccgcat tgcagttggc gggcaacacg ccgcataccg cagtcgtcaa
    13201 cctgtacggc gaaatcggca acggtgtaga agaccaggtc aaaaaactca aagacggtat
    13261 ggaggccgcc tacaaaaacc cgcaggcaaa agccatcgtc atccgcgcca acagccccgg
    13321 cggttcgccc gtcgtctcca acaccgcttt tgaagaaata cgccgtctga aggcgcagca
    13381 ccccggcatt cccgtttatc tcgtggcgga agatatgtgc gcgtccggct gctactacat
    13441 cgcggcggcg gcggacaaaa tctatgccga cccgtccagc atcgtcggca gcatcggcgt
    13501 tatcggcagc agtttcgacg cgaccggcct gatggaaaaa atcggcgtga aacgcagggt
    13561 taaaattgca ggcagcaaca aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca
    13621 gtcgaaaatc tgggaagaaa tgctgaccgg catacacggc gaattcatca aagccgtcaa
    13681 aaccggacgg ggcggccgcc tcaaattccg acaatatccc gacgtgttca gcggtcgcgt
    13741 gtacacaggc gcggatgcgc tgaaagtcgg gctggtggac ggactcggca atatttacag
    13801 cgtggcgcga gacgtggtca aagcccccga tgtggtggat tacactccga aggacgattt
    13861 cggcagaatc ttgggcagac gcttcggcgc agaactcaaa gcaagcgtgc gcgaagccct
    13921 gcaggccgtc cggtagcgtt gtttacagta tttctaaggg aataccaaaa taaaaatata
    13981 cacaaaaaca ctcacctccc cagtatgctg ccactaagtc agccgcgtca ctatttaccg
    14041 tgcacttaaa gccgtaaaag gcaggttgtc cccgggaggt tttgtgcatc ctgataccga
    14101 tcggttgttg ttgctcaaag gacagaaggc cgctgataaa cgagattacc tgtttgtcgc
    14161 tattgacgat ttttatactc tgccattttg ccagacaaaa ccgcagacag tgctgccaag
    14221 tttctgaccg aacatctggc cgacccctgc ttgtacctga ttgagtacgt ttactctgac
    14281 aatgataggt aatataaaga gccgtccaac atgctttcgg tgcagcttgt tataataatg
    14341 ggattggttg gaggcttgcc cgatttgctt gtccgcagac caacggtaag gcggagcggg
    14401 ttatccgtac cttgatggag atgtggcatg aggaacagtc gtttgacagt ccggaacatc
    14461 ggcaaaagga gttgtgctgc tttattaatt taggggctgc cctagatcac tagggaaatt
    14521 caaattaagt tagaattatc cctatgagag aaagtcgtct aagccggtat aaacaaaata
    14581 aactcatttt gttatcgtgg ctatgatgta ttggatgcgg gcgaatttag ccatttccgt
    14641 atcaatcaca gcacacattt tgccgaacga caaaaccata ttaatggaa
//
LOCUS       DAWFJW010000046        14572 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFJW010000046 DAWFJW010000000
VERSION     DAWFJW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.046"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(71..553)
                     /locus_tag="WGR19_001115"
     CDS             complement(71..553)
                     /locus_tag="WGR19_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0798654.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(706..867)
                     /locus_tag="WGR19_001116"
     CDS             complement(706..867)
                     /locus_tag="WGR19_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798655.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(941..1402)
                     /gene="recX"
                     /locus_tag="WGR19_001117"
     CDS             complement(941..1402)
                     /gene="recX"
                     /locus_tag="WGR19_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0798656.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1475..2185)
                     /locus_tag="WGR19_001118"
     CDS             complement(1475..2185)
                     /locus_tag="WGR19_001118"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0798657.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2592..3857
                     /locus_tag="WGR19_001119"
     CDS             2592..3857
                     /locus_tag="WGR19_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0798658.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3915..4721)
                     /locus_tag="WGR19_001120"
     CDS             complement(3915..4721)
                     /locus_tag="WGR19_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0798659.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            5066..5275
                     /locus_tag="WGR19_001121"
     CDS             5066..5275
                     /locus_tag="WGR19_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0798660.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            5364..6578
                     /locus_tag="WGR19_001122"
     CDS             5364..6578
                     /locus_tag="WGR19_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0798661.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(6665..9244)
                     /gene="clpB"
                     /locus_tag="WGR19_001123"
     CDS             complement(6665..9244)
                     /gene="clpB"
                     /locus_tag="WGR19_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0798662.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLNSLPKVSSQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            9504..10514
                     /gene="trpS"
                     /locus_tag="WGR19_001124"
     CDS             9504..10514
                     /gene="trpS"
                     /locus_tag="WGR19_001124"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0798663.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRSAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            10583..11128
                     /locus_tag="WGR19_001125"
     CDS             10583..11128
                     /locus_tag="WGR19_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798664.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNVVRLTGKHPGDLEAVGGKCME
                     TDGKGAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            11448..11792
                     /locus_tag="WGR19_001126"
     CDS             11448..11792
                     /locus_tag="WGR19_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798665.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAAKDTLNKAADAT
                     QEAADKMKDAAK"
     gene            complement(12001..13509)
                     /locus_tag="WGR19_001127"
     CDS             complement(12001..13509)
                     /locus_tag="WGR19_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEZ0798666.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFLIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(13625..14357)
                     /locus_tag="WGR19_001128"
                     /pseudo
     CDS             complement(13625..14357)
                     /locus_tag="WGR19_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         3294 a         3629 c         3993 g         3656 t
ORIGIN      
        1 ttgatttcat ccgtttgcaa tggaaacgga tggcgcgatg tgcctgtctt tcagacggca
       61 tagtccgccg tcagcagccg caggacatgt cttcagaggc ttgcaggctg atgtctctgc
      121 gttttttggc tttttcgtag cggcagcgtt ggcggtcggt caggatttcc agcggaggga
      181 cagggacggg tttgccgtct ttgaccgcaa ccatcgtgaa gtagcagctg ttggtatggc
      241 ggatttcgcc ggtgcggatg ttttgtgctt cgacacggat gccgatttcc atcgaggtgc
      301 gtcccgtgta gttgacagcg gcgtaaaagg taaccaaatc gccgatgtgg atgggttctt
      361 taaacaggac tttgtcaacc gacagggtaa cgcaataatt gccgctgtaa cggctggcgc
      421 aggaataggc gacttggtcg agcaggagca ggagttcgcc gccgtgtacg ttgccgctga
      481 aattggcggt gtccggcatc atcagttcgg acataatgag ttcgtgcgaa ggcagttggc
      541 gttgttgtgt catggtgtac ctgttttaag aaagggtgtt ttgaaatggg tgcggggaaa
      601 ttttagggaa tggggaagtg gaattcaagc taaaaaaagc tatggcggga tagtctgcag
      661 aaatgggccg ccgatgccgt ctgaagcctt cagacggcat tttgtttagg aaagccaaaa
      721 acggaagacc aggaaaaaca cgagtgccaa tacggcgacc caaacgacga gcatgaatcc
      781 ggcaactgcc cgtcccatac ggtaaaccgg gtttttgcgt tcgtcttcga tgggggaggt
      841 gtggtgtttt tcgggttgtc cggacatttt gttctcaatc ggaaaagttg gaaacgggcg
      901 taaggttatg ttgccgtgca aaaagattca agaaggaagg tcagcagccg tcttcccaat
      961 tttcgtccca agcgtgtttc aatgccgtct gaacggtatc ggcatcgaat ccgcgatagg
     1021 cgaggaaacg cgcctgtttt tgtttttctt tgaggtcggc ggcggggtgt ttgaattttt
     1081 tacgcaacac gtctatggcg gcctgttttt cgcttgagcg gtcgggaagc aggttgcggc
     1141 tggtttcttc atctatgccc tgttgcgcca aagcctgttt cagcctcaat gaaccgtgtt
     1201 tgcggctttt gctgcggata taggcttcgg catagcgcaa atccgactgc cagttgcgtt
     1261 cggcaaattc gtttaacacg ttttccaact cctcttcgct ttcggcgtgc ggtgcaagtt
     1321 tgcgtttcag accgatgcgg ctgacttctt ggcgcgagag gatgtccatc gcgcgggcgc
     1381 gtagggattt ttgcggtttc atggcggcgg tttcggcacg gaaagattca gacggcatgg
     1441 gaaacggatt ccgatgccgt ctgaacagat tgttttattc ttcgtcttta tttttctgcg
     1501 gttgcaggtt ttcgtaaatt tcgggcagcg agccgataat ccagtcgggg cgggtcgcat
     1561 cgtcttgcga gagcagcgtc atatcgccgt aaccgaaggt aacgccgacg ctcaggcagc
     1621 cggcggcttt ggcggcgatg atgtcgttgc gcgagtcgcc gaccataacc atgtttgcaa
     1681 catcgatacc caaaacttcg gcggcgtgcc gcagcggcag ggggctgggt tttttctcgg
     1741 gcaggctgtc gccgccgagt atcaggctga agtagtcggc aagtccgagc tgtttcaaca
     1801 gttcgacggc aaggatttcg tttttgttgg taatgactgc cagcgggatg cccaaagatt
     1861 tgagcaatgc cagcccggct tcggtttcgg gataggggcg ggtgaagacg ctcaaatggt
     1921 cgcggtagta tttcatatag gatacgaaac ctttttccca cagttcggaa tcggcttcgc
     1981 ggtcgcggtc gttggtgagg acgcggtgaa ccagtttgcc gatgccgtcg cccacatagc
     2041 tttcgaccac tttggcgggc agcggtttca taccgagttg ttccaacatc gcttctgcgg
     2101 cggcggcaag gtcggggacg gaatcgcaca gtgtgccgtc caaatcgaag gcgacggctt
     2161 ggacgtgttc gatggctgca ttcataatct gatttcctgt cgggttgaaa gggtttattt
     2221 taacatcgtt tgcacagggc ttctaatgta tggattgtta acaatcgcaa caggttttgc
     2281 aggcggtgtt ttcggatttc ggggcggaat cgggcaaggg tttgaaatcc cagcgatgtt
     2341 ccaattaaat ttttaaaaaa taaaaggatg cctatatctt tatttaattt gacatttatt
     2401 tttcggacgc gcgcgctgct ttttcaggcg gagtccgggc tgattccgtg cggcttgaaa
     2461 aataaaatca tatatagatt aatcaaatcg ggcttatgcc gactacacgc tattacattt
     2521 tgtcgggaaa ttggttatag taatgagcga aatgcacgcc cctgaaaatc tttttgtgaa
     2581 aaggaagcaa aatgtctgaa gcccttgcaa aagaaaccct caatccgttt gaaatcgcgc
     2641 gcaaacaggt caaaaccgcc tgcgaccggc tgaaaaccga tccggccgtt tatgaaatcc
     2701 tgaagagtcc gacccgcgtg ttggaagtca attttcccgt caaactcgac gacgacacgg
     2761 tcaaaacctt cacgggctac cgttcgcaac acaacaacgc cgtcggcccc tataaaggcg
     2821 gcgtgcgctt ccatcccgga gtgaatctgg acgaagtcaa agccctgtcg atttggatga
     2881 ccatcaaatg ctgcgtagcg ggcattcctt acggcggcgg caaaggcggc gttaccttgg
     2941 atccgcgcga ttattccgag gcggaactgg aacgcatcgc ccgcgcttat tccgaagcca
     3001 tttctccgct gatcggcgag aaaatcgata ttcccgcacc tgatgtgaac accaacggca
     3061 aaatcatgtc ttggatggtt gatgcctatg aaaacgtggt gaaacattct gcaccgggcg
     3121 tatttaccgg taaaccggtt gagttcggcg gctctttggc gcgtaccgag gctaccggtt
     3181 acggcgtgaa cctcgccgcc gtccaagcct tggaaaaact gggcaaagac gtgaagggtg
     3241 ccacttacgc catccaaggc ttcggtaatg tcggttacca cacaggttat tacgcgcatc
     3301 aatccggcgc gaaagttgtt gcggtttcta ctgttgacgt tgccatttac aacgaaaacg
     3361 gcttggatat ggaagctttg tttaaagagt tccaagaaaa aggcttcatc accaacgaag
     3421 ccggttacgg caaggaaatt accaacgccg aacttttggc tttggatgtg gacgtactcg
     3481 ccccttgcgc gctggaaaat caactgactt ctgaaaacgc cggtaaagtc cgcgcgaaaa
     3541 tcgtggtcga aggcgcgaac ggcccgacta cgcccgaagc cgacgttatc ctgcgtcaaa
     3601 acggcgtatt ggtcgtgccc gatattttgg cgaactgcgg cggcgtggtg gtatcttatt
     3661 ttgaatgggt acaaaatctg caaggctatt attgggagtt tgacgaagtt caggaaaaag
     3721 aaaccgtcgt cctgcgccgt gcgttccgcg atatatggaa cttggcgcaa gagtatgatg
     3781 tcgatttgcg aaccgcgtct tacatgatga gtatccgccg tgttgaaaaa gcgatgaaac
     3841 tgcgcggttg gtattgatgt ttgaacaaaa aatgccgtct gaagccttca gacggcattt
     3901 ttgtgcaggc gggattaatg gtgcgaatgg ccgatttgga aggttaaagt cgagtagttc
     3961 gcctgttttt ggcacacgct ttgatcgggg aagtcggctt tgtgttcgac attggctttc
     4021 cagaagcctt ggcgcaaggg gatgatgtcc acttcgcctt tgtcgtctgt gctgtcggag
     4081 aaagcctgtg cttcggtttt gtgcgttttg ctgcggtcgc tggtgtcgaa gccgtcaaag
     4141 gtggcggtaa cggtggcatt gggcagcggt tcgccacgga acagaacgcg gactttgaag
     4201 cgttcgccta cgtgaatgtt ggcgggattg tccagcggga cgatttccaa gttttgtccg
     4261 accggtttgg tgatgatggc ggtgtccgcg ctttcgtgtc cgacgttgac gatgtttttg
     4321 ccgaacattc gggtttgttc gcaatagctt gcgtcaggca tttctttgat gcccgcctgt
     4381 ttccagcctg ctttgttttt tgaccagaaa gtaggctgat attcggcgat gacgaggtaa
     4441 ctgccgtcct taacgggacg gttgcttcgg tactggtagt tgtatgtgcc gcgttgaatc
     4501 atgttttcct tgcctttttc ggtaaccagc tgcatcggtt tgctgaagat gtgcaggcgg
     4561 tctttggcga tgggttcgag ttcgggaaat tcgccgtagc ccaagtcggc tttaaggtat
     4621 tcgccgccgt gcgtgtgggc ggtttcgacc cagacgcggt gggcgtgggc ggatgtggca
     4681 aatagggcgg aaacggcgag cagtgtcaat gttttcttca tggtgcagcc tttgtctcgt
     4741 gtgggtcaaa gattttgtta tgttataaca aaataggtat cgtgtgaaga cggatttgct
     4801 gtctgaaaac tgtctttttg tatagtggat taacaaaaac cagtacggcg ttgcctcgcc
     4861 ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact
     4921 atttgtactg tatgcggctt cgtcgccttg tcctgatttt tgttaatccg ctatatcgat
     4981 ttgaattttt cagaaaatga agcggacgtt tgggcggagg caacttgttt gataagatag
     5041 caatatttca aaacggagaa agatcatgcc ttatgtcaat attaaagtaa ccggcggcaa
     5101 ggaagcaccg actgccgcgc aaaaagcgga actgatcggc ggcgtaaccg aattgctggc
     5161 acgcgtgctg ggcaaaaatc ccgaaacaac ggttgtcgtg attgatgaag tggataccga
     5221 taactgggga ataggcggca aaagcgtcag cgaacggcgc aaagagggca ggtaaaaagc
     5281 ctgaaaatct cggtttgatg ctttaaattc cgcgtgaaaa agagtacatt cccacccatt
     5341 gcccaaaatt tacggaacac atcatggata aatttcccaa gtctgcaaag ctcgatcacg
     5401 tctgttacga catacgcggg ccggttcaca aaaaagccct tcagttggaa gaggagggca
     5461 ataaaatcct taaactcaat atcggcaacc ctgcgccgtt cggctttgaa gcccctgatg
     5521 aaatcttggt cgatgtcatc cgcaacctgc cgacttcgca aggctattgc gattctaaag
     5581 ggctgtattc cgcccgcaaa gccattgttc actactatca gaccaaaggt ttgcgcgata
     5641 ttacggttga tgatgtctat atcggcaacg gcgtgtccga gctgattacg atgtctatgc
     5701 aggcattgct caacgacggc gacgaaatcc tgattcccgc gcccgactac cctttgtgga
     5761 cggcggcggc aacgcttgcg ggcggtacgg tacgccatta tctgtgcgac gaagaaaacg
     5821 gctggttccc caaccttgcc gatatggaag ccaaaatcac gcccaaaacc aaagccatcg
     5881 tcgtcatcaa tcccaataat ccgacaggcg cggtgtacag cagggaaatc ctgttggaaa
     5941 tcgccgaact ggcgcgcaag cacggtttga ttattttcgc cgacgagatt tacgataaaa
     6001 tcctttatga cggcgcggtt caccaccaca tcgccgcgct tgcccccgac cttttgacgg
     6061 taacgttcaa cggtttgtcc aaatcctacc gcgtcgccgg cttccgccaa ggctggatgg
     6121 tgctcaacgg gccgaaacat cacgcaaaag gttacatcga gggtttggat atgctctcgt
     6181 ctatgcgcct gtgtgccaat acgccgatgc agcacgcgat tcagacggca ttgggcggct
     6241 atcagagcat caacgaattt attttgcccg gcgggcggct tttggaacag cgcaacaggg
     6301 cgtgggaact ggtcagccaa atccccggcg tgtcctgcgt caaaccgatg ggcgcgatgt
     6361 atatgttccc aaaaatcgat accgaaatgt accgtatcca cgatgacatg aaattcgttt
     6421 acgatttgct ggtgcgcgaa aaagtcttgc tggtacaggg aacggggttt aattggatta
     6481 aacctgacca tttccgcatt gtcacgctgc cttacgtcca tcagattgaa gaggcgatgg
     6541 gcaggttggc aagattcctg caaacctacc gccaataagg ggacggtttg tctgccgagg
     6601 ataaaaaatg ccgtctgaaa cggagattcc cgtttcagac ggcattttca acagcaggaa
     6661 cgaatcaggc aaatttcagt ctgtcgccgt cggcttctac cctgatttcg ctttcgggcg
     6721 catagtttcc ggcaagcagg gcttttgcca gcgggttttc gatttccgac tggatggcgc
     6781 gtttgagcgg acgtgcgccg taaatcgggt cgaaaccggc tttggcgatg atgtccagtg
     6841 cggcatcgga aacagtcagg cgcaggtttt gtttttccaa acgtttttcc aaacctttga
     6901 gctggatttt cgcaatgctg cggatgtttg cctgatccaa accgtggaac acgaccactt
     6961 cgtcgatgcg gttgatcatt tcaggacgga aatgttcttt cacatcctcc atcacaactt
     7021 cttttaccgc ttcgtaatcc tgaatgccca tttgttggat gtgctggctg ccgatgttgg
     7081 aagtcatcac aatcacggta tttttaaaat ctaccgtcct gccttgcccg tcggtcaaac
     7141 gaccgtcatc caatacttgc agcaggatgt tgaacacatc gggatgggct ttttccactt
     7201 cgtccagcag aatcacgctg tacggtttgc ggcgcacttg ttcggtcagg tagccgcctt
     7261 cttcgtagcc gacatagccc ggaggcgcac cgatcaggcg tgcaacggcg tgtttttcca
     7321 tatattcgga catatcgatg cgaatcagat gatcttcgct gtcgaacaga aagcctgcca
     7381 gggctttaca caactcggtt ttacccacgc cggtcgggcc caagaacagg aagctgccgt
     7441 aaggcttgtt cggatcggca agaccggagc ggctgcggcg gatagcgtcg gacacggcac
     7501 gcacggcttc gtcctgtccg accacgcggc ggtgcaatac ttcttccatt ttcagcagtt
     7561 tgtcgcgttc gccttccatc attttggata cgggaatgcc ggtcatacgg gaaaccacct
     7621 ctgcgatttc ctctgcgccg acattattac gcaagagttt gtttgccggt tttgtgctgt
     7681 ccgtatctgc ccgttcggcg gctgcacgct gtttttccaa atgctccaaa tcttcataca
     7741 tcaattttga agccagtgcc aaatcgcctt gccgttttgc ctgttcgatt ttaattttga
     7801 cttcgtcaat ttgtttctta atattagcag caccgtctga aattgctttt tcggctttcc
     7861 agatttcgtc taaatcggcg tattcttttt gcagaccgtt gatttcctcg tctatcagtt
     7921 ccaaacgttt tttgctggca tcgtcttttt ctttttcaac gtgcgccttt tccatccgaa
     7981 gctgaattag acggcggtcg attttgtcca ttgcttccgg cttggtttct ttttccatct
     8041 tgacacggct ggcggcttcg tcaatcaaat caatcgcttt atcaggcagg aagcggtcgg
     8101 taatgtagcg gtcgctcaac tccgctgcgg caacgatagc agggtcggta atatcgatac
     8161 cgtggtggat ttcataacgc tcctgtaaac cgcgcaagat agcgatggtg tcttctacac
     8221 ttggctcgcc gaccaatact ttttggaagc ggcgttcgag tgccgcgtct ttttcgatgt
     8281 attgacggta ttcgtccaag gtggtcgcgc cgatacagtg cagttcgccc cgcgccaagg
     8341 ccggtttcag catattgccc gcgtccatcg cgccgtcggt tttgcccgcg ccgaccaaag
     8401 tatggatttc atcaatgaaa atcagagtgt tgccgtcgtc tttcgccaaa tcgttcaaca
     8461 cgcctttcaa gcgttcttca aattcgccgc ggtatttcgc gccggcaatc aaagccgcca
     8521 aatccaaaac cagcaggcgt ttgttacgca gggattcagg tacttcgccg ttgacgatac
     8581 gttgcgccaa gccttcaaca atggcggttt tacccacacc cggttcgcca atcagcacgg
     8641 ggttgttttt ggtacggcgt tgcaatacct gaatcgcgcg gcggatttcg tcgtcacggc
     8701 cgataacggg gtcaagtttg ccgtcgcggg cgcgctgggt caggtcaagc gtatattttt
     8761 tcagcgcatc gcgttggtct tcggcattgg catcgttcac gttttgtcct cctcgtaccg
     8821 catcgattgc ggcattgatg ttttgttcgg tcgcgccggc ttctttcaaa attttgccgg
     8881 ccgcatcgtt ctgctgcacc aaggcaagca ggaaaagttc actggcgata taggcatcgc
     8941 cgcgtttggt tgcggctttg tccatcaggt tcaacaccgc ctgcaattcg cggctgggca
     9001 gaatgtcgcc gccctggctg gacactttcg gcaggctgtt caaatgctgc tgcaaacgct
     9061 gtttcacctg cggcacgttc acgcccgaat gcgccagcaa cgcggcggct ccgctgtttt
     9121 ggtcgtcaag cagggctttc agcacaaagc ccgcttccag atagctgccg tccgcagcca
     9181 acgccaaact ctgagcttct gcaagggctt gttggaattt ggcggttaat ttgtcgtatc
     9241 gcatttttgt ttccttttca aaatgtccgc tgtcgaagcc tatatgtgca taattgtgga
     9301 taactcaagt tctgttttct gtttttctat atttaattcg atatatcatt gaatttaaag
     9361 tatataaaaa tgtataataa tgtgtataac tatatcttct taatatggaa aagtctgttg
     9421 tcggctggat gtaggtggca aatcgggtat aatcggcgca tctttttccc tttcagacgg
     9481 cattgatgcc gcaaggacat tttatgagca aaaaacgagt tctgaccggc gtaaccacca
     9541 ccggcatccc gcatctgggc aactacgtcg gtgccatccg ccccgccgtc cgctcggcgc
     9601 aaaaccccga taccgaatcc ttcctcttcc tcgccgacta ccacggcatc atcaaatgcc
     9661 acgagccgga gatgattcac caatccaccc aagccgttgc cgccacttgg cttgcctgcg
     9721 gactcgaccc cgagcgcacc accttctacc gccaaagcga cattcctgaa gtgatggaat
     9781 tgaactggat tctgacctgc atcaccgcca agggtttgat gaaccgcgcc cacgcctaca
     9841 aagccgccgt gcaggcaaat gcggaaaacg ggcaggaaga ccccgatttc ggcgtggaaa
     9901 tgggtttgtt cagctatccg attctgatga ctgccgacat ccttatgttc aacgccaacg
     9961 aagtccccgt cgggcgcgac caaatccaac acgtcgaaat ggcgcgcgac atcgccggcc
    10021 gcttcaacca ccgcttccga gaactcttca ccctgcccga agtgaaaatc gatgaaaacg
    10081 tcgaactctt ggtcggtttg gacggacgca aaatgtccaa atcctacggc aacaccattc
    10141 cgctttggga aaacgacaaa aaaacccaaa aatcggtcaa caaaatcatc accaatatga
    10201 aagagccggg cgagccgaaa cagcccgacg aaagcccatt gtttgaaatc tacaaagcct
    10261 tctccacgcc gtctgaaacg gtggaattta cgaaaatgct tgccgacggc ctggcttggg
    10321 gcgaggccaa aaaacttttg gcggcgaaaa tcaacgccga actcgccgaa ccgcgcgaac
    10381 gctacaacga gctgaccgcc gacccttctc aaatcgaaga gattttgcag gcaggcgcgg
    10441 cgaaagcgcg taaagaagcg cgcgaattat tggacaaagt acgcgatgcg gtcggcatcc
    10501 gcccgttgaa atgaacccga tgccgtccga acctccgcct gccgcgtttc agacggcatt
    10561 ttgaaaccat caggagtgtg gaatgcttaa aacatctttt gccgtgttgg gcggctgcct
    10621 gctgcttgcc gcttgcggca aatccgaaaa tacggcggaa cagccgcaaa atgcggtaca
    10681 aagcgcgccg aaaccggttt tcaaagtcaa atacatcgac aatacggcga ttgccggttt
    10741 ggctttggga caaagcagcg aaggcaaaac caacgacggc aaaaaacaaa tcagttatcc
    10801 gattaaaggc ttgccggaac aaaacgtcgt ccggctgacc ggaaagcatc ccggcgactt
    10861 ggaagccgtc ggcggcaaat gtatggaaac cgacggaaag ggcgcgcctt cgggctgggc
    10921 ggaaaacggc gtgtgccata ccttgtttgc caaactggtg ggcaatatcg ccgaagacgg
    10981 cggcaaactg acggattacc tgatttcgca ttccgccctg caaccctatc aggcaggcaa
    11041 aagcggctat gccgccgtgc agaacggacg ctatgtgctg gaaatcgaca gcgagggggc
    11101 gttttatttc cgccgccgcc attattgagg tattcggaca tcccggaata tatttgggtt
    11161 tttcaaacct tgcgaaaaaa agtccgcacc gcagggaaac tcaaaaggag ggggatatgt
    11221 gttacaattt tccgaactgt tttcataaaa tagttttcgg acgtgtttca atatggcatt
    11281 gatgccgcct tattgttcga gaaaaaaacc tttatattta aatataatgg gttttaacta
    11341 aacgggaaac cgttttctct ccggccgatg ggcaaaatca gccgattgat ggaacacggt
    11401 tcgattttta agcaaaacct taatcaacaa ctcaagaagg ataagacatg aaaaaattat
    11461 tgattgccgc aatgatggcg gctgccttgg cagcttgttc gcaagaagcc aaacaggagg
    11521 ttaaggaagc ggttcaagcc gttgagtccg atgttaaaga cactgcggct tctgccgccg
    11581 agtctgccgc ttctgccgtc gaagaagcga aagaccaagt caaagatgct gcggctgatg
    11641 caaaggcaag tgccgaggaa gctgtaactg aagccaaaga agctgtaact gaagccaaag
    11701 aagctgtaac tgaagcagct aaagatactt tgaacaaagc tgccgacgcg actcaggaag
    11761 cggcagacaa aatgaaagat gccgccaaat aatttgttgc cttggcaaaa atgatgggat
    11821 gccgcctgcc ggcaacccaa acgaaaccgc ctgaagattt tcaggcggtt tttgagttgt
    11881 atgccccttg ttttttacgc ctgcatgacc gttccgagca gatgtgccgc gcatatcggg
    11941 atgttgcgga caggcaaatg ccgtctggac agggtttgga cggcatttgt tccgtaaggt
    12001 tcagacgacc tcgactttaa acggcatatt gattttttgc cattggacgc tttcatattc
    12061 caatgcgtcg gcaatcaggg gatggcgttc cagccattcc ctgtcaatac gcaggatgaa
    12121 gccgcagctt tccgtatccg tgcgcaactg catatttttc gggaaagaca ggtcttggcg
    12181 cgaacggcag aacagggcag caaggcgcag ggacaaaacg gcataccaca acatttcgtt
    12241 ggtgccgatg atgccgctca tttttttcat atcgccgcga tgaccaatga ccagttgggc
    12301 aagtatggtc tgttctttgc gtgagaaacc cggcatatcg gcgttttcga ggatgtaggc
    12361 ggaatgcttg tgatagccgg tgtgggcgat gtccaaaccg atttcgtgca gcgcggcggc
    12421 gcgtccgaga tactgttgcc acaaggcaag ctcttgaact gtaacgtttt tagcgtggca
    12481 gaggctgtcc ataaaggttt gcgcggtctc ggcggtgcgt ttcgcctgat tgaggctgac
    12541 gtggtagcgg tgttggaact cggcaaccgt ttgtccgcgc atatcttcgt ttaaaccgcg
    12601 cccgatcaaa tcgtaaaaca cgccgtcgcg cagggcggct tcggttacgg tcatcctgtc
    12661 gagtttcatt tcctcaaacg ccgccatcat cacggcaagt ccgccggcaa aaacttcgat
    12721 gcgttccggt ttcaggtttt caaatttggc ttttttgacc gaaccggctt cgatgatgcg
    12781 ttcggcgagg gcgcgcattc ctttgtaggt aatgtccgcc tcttggggca tttcggcggc
    12841 aagcacgtcg cggatggatt ttgccgaacc cgatgtgccg acggcgaaat cccaaccttc
    12901 gcgcctcata tttttgctga tgcgctggat ttcgttgcgg gcggcggaaa tggcagattg
    12961 gaagtctttg gcggtgattt tgttttggaa gaagcgcagg ctgtaggtta cgcagcccaa
    13021 gggcaggctt tcagtaatgt cgggattcag cgtcgagccg atgacaaatt ctgtcgaacc
    13081 gcccccgatg tcgataacca gcattttgcc gccgccgggg gggagggtgt ggatcacgcc
    13141 ggtataaatc agccgtgcct cttcgcgccc ggcgatgatt tctatgagga aacccagtgc
    13201 cgcttcggcc ttgggaagga aatctgcgat gtttttggcg acgcggaatg tattggttgc
    13261 cacggcgcgt acctgttcag ggcggaagcc gcgcaggcgt tcgccgaatt ttgccagaca
    13321 gtccaaagcc tgttcttggg aagcggcact caggtttttc tgttcgtcca gaccggcggc
    13381 gaagcgcacc atctgtttga acgaatcgat gacttttaat tgtccgttgt tgttttcgca
    13441 aatctggagg cggaaactgt tggaacccaa atcgacggag gcgaggatgt ttgcgggggt
    13501 ggtggtcatg gcggatatcg gtgggggaaa aacgcaatgt tactctgacg gcgcaggcgt
    13561 tgacaataaa tgatgcggcg gtttttgatt ctgcccacgg atgttgccga cggcattttt
    13621 gcgcttattt gaaatccttt tccacgctca tgaaaatctg cgtgtttttg cgtgtgtaaa
    13681 aacttttcat attgctgtcg attttcagat agcggaaatt gagttgcggc gtaaagccct
    13741 tccaagagat tttgtcgtgc cacaacgaca ggtttgcctg atattcgtgg tctttgcgcg
    13801 ggaagcggta cacaatggtc ccgggtgcgt caaaagtcct gcgggtatag cgcaggtttg
    13861 cccgcagacc caagcccgcc gtcgaacgtt ttgactgcgc cgacacgcaa acccttgcgg
    13921 acggaagcct gttccgcctc tttcgtcatg ttgtgcgacc agtccgcacc gccgtaaagc
    13981 agccagtctt tcggcgcgga atacatcagc gtcgcgcccg ccagcggcat atggctgtcg
    14041 tatcgggcgg cggtgcggtc ttcctgataa cgcttccaca tattgcccac gtttagtgtc
    14101 agccgccagc gttcgctcaa gcgttgggag aaatcggcat tgaagccgcc gacgaaattg
    14161 tatcggctgc cgcctaagag gttttgcccg acaaacggca cgatgccgaa cgagcgcgtt
    14221 accgaacgat ttttatagcc gagcgacagg cgcaggcact gttcgctgaa atctttgtta
    14281 tcccaataat gcacgccgcc gccgctgatg ccgccgtaga ggaaatgatg cccgcccgca
    14341 ttgatttcgc gcgacacgcc taagccctag cgcaaaccgt gtgccttttg cggcaggctg
    14401 tcggcggttt tcgtccagct tctgcccgca aattcaatcg tttttttgga cgaagcgttg
    14461 tttatagcgg attaacaaaa atcaggacaa ggcgacgaag cctcagacag tacagatagt
    14521 acggaaccga ttcactcggt gcttcagcac cttagagaat cgttctcttc ga
//
LOCUS       DAWFJW010000047        14541 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFJW010000047 DAWFJW010000000
VERSION     DAWFJW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.047"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..1409)
                     /locus_tag="WGR19_001129"
     CDS             complement(<1..1409)
                     /locus_tag="WGR19_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0798667.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKSVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQ"
     gene            1594..3021
                     /locus_tag="WGR19_001130"
     CDS             1594..3021
                     /locus_tag="WGR19_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0798668.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIIKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3012..3254
                     /locus_tag="WGR19_001131"
                     /pseudo
     CDS             3012..3254
                     /locus_tag="WGR19_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3163..4272
                     /gene="mltB"
                     /locus_tag="WGR19_001132"
     CDS             3163..4272
                     /gene="mltB"
                     /locus_tag="WGR19_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0798669.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKMLV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(4395..6401)
                     /locus_tag="WGR19_001133"
     CDS             complement(4395..6401)
                     /locus_tag="WGR19_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0798670.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGSLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            6690..8717
                     /locus_tag="WGR19_001134"
     CDS             6690..8717
                     /locus_tag="WGR19_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0798671.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGNLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(8808..10361)
                     /locus_tag="WGR19_001135"
     CDS             complement(8808..10361)
                     /locus_tag="WGR19_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0798672.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            10412..10588
                     /locus_tag="WGR19_001136"
     CDS             10412..10588
                     /locus_tag="WGR19_001136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798673.1"
                     /translation="MPSENGRRQFIIFAFRRHIERRLCAVCRRFACVFPMSEANAGCV
                     RQTVRGVAMPSEAI"
     gene            complement(10648..11841)
                     /locus_tag="WGR19_001137"
     CDS             complement(10648..11841)
                     /locus_tag="WGR19_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0798674.1"
                     /translation="MNSKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRSAAADFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRVSLFTKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQHMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(11838..12821)
                     /locus_tag="WGR19_001138"
     CDS             complement(11838..12821)
                     /locus_tag="WGR19_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0798675.1"
                     /translation="MKHFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYAFSDGIKTFLSGETPPTAQDGGSADMPSEAATSEAVPQTGET
                     EWKQDTDTAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     HTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(12832..14268)
                     /locus_tag="WGR19_001139"
     CDS             complement(12832..14268)
                     /locus_tag="WGR19_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0798676.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRHPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFSLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGTLHGTALVLLNTGDRYFGRNALCRLKYLAPLSWLITFHFVCLTFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQADMLLLASFASVMLLYPYLQRAFDDAVKGLEKIPMWL
                     WFIPVSAVLLLVIVLAPSGIPGFIYANF"
     gene            complement(14398..>14541)
                     /locus_tag="WGR19_001140"
     CDS             complement(14398..>14541)
                     /locus_tag="WGR19_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ0798677.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLTAAAGLWVCLNLNRH
                     IRT"
BASE COUNT         3112 a         3653 c         4429 g         3347 t
ORIGIN      
        1 ctttgttccc tctcctgtgg gagagggcta gggagagggt ttgttgcgga tgcggaaaat
       61 tcggtttggt cggtaaccgc atcgccttct ccttcggagg cggaagtagt ttgctgctgg
      121 ttcggggatt gcaatccgcc cagtttgaat ccgtaagcca acggtgccac tgtaatcatc
      181 agcggctcat gtgccgacaa gaagccttgc caatcagaca cgcttttggc ttttaaaccg
      241 ttttgctgca agaagccgag catggtttcg cgccgtccca aactttcggc gcacagcaaa
      301 atccgtcctt caaacgccgt ctgaaaatcc ttcaatgcct gcaaaggctc gtcggattgt
      361 cggttgacgg caaggtcggg gagggcgtgt tccttgccgg aaacatcggg cagcacctgt
      421 ccgtagtttt tcagacggcc tgcaaaaaca tcggcagaga gatacaaatg ctgtggaagc
      481 aaaggcggat aggtttcgtc gccctgcgcc atggcgtaac gcgatttgac gtcgctccaa
      541 aagcggtttg cctcggcgtg aacatcgccc aaagagacaa acagcgcgtc ttcgccgata
      601 tagtcaaaca gcgtttccag ctcgttttca aaaaacagcg gcaggtaata ttccacgccc
      661 gcaccgaagt ggccgttgct tactgctttg tatacagccg catcgttcgg attaccatcg
      721 acttcctcgc ggaagcggct gcggaagatt ttttgcgcct cgctgtcggt ggggaattcg
      781 tgcgccggca gcaggcggat ttcggaaacg ggggagatgg tgcgctgcgt gtccgtatca
      841 aaggttttga tgctgtcgat ttcatcgtca aacaaatcga tgcggtaggg catttcgctg
      901 cccatgggga acaaatcgac aatgccgccg cgcacggcaa actcgcccgc cgcaacaaca
      961 tgggaaacat ggttgtagcc cgcatctacc aaatcggttt tcagacggcc tatatccaaa
     1021 gtctgccctg ttttcagcca aaacgtgcgc cctgccagaa acggcacggg cggcagcttc
     1081 tgcatcgccg tggcaaccgg cacaaacaac acatccgccg cgccgctctt aatctgccac
     1141 aacgccgaca gccgttccga caccaaatcc tgatgcggcg aaaaacgctc gtaaggcagc
     1201 gtttcccagt ccggcaggaa caccgccgtg tcgtgcggac ggaaaaaccg ccatgccgtc
     1261 tgaaggcgca acgcctgttc cgcatcttgg gtcagcacga ccttgagccg cttgtgcggc
     1321 agataacgcg ccaaagccaa gggcagcgag ccttgcgaaa gattggacca acgggatttt
     1381 tcacggggtt tggggatggg gtaggtcata gtgcggcaat cggcggacag tgatgcaaac
     1441 ggtcatttta gcacgggagg gggcgcgatg ccgactgaaa ggtcggcgga ttgttgcccg
     1501 gcgcactttg tggtaaagtt tagaaaatca actctaaact gtggcaggcg gtttgaccgg
     1561 ccgccgatta atgggaaccg acagaggaag ccgatgatac acaccgaacc gagcgcgcag
     1621 ccgtcaacta tggacacggc tgctttttta aagcacatcg aatccgcatt ccgccgcatt
     1681 ttttcagacg gtatcgacct gatgcgatac ctgcccgaag acaaatggct tgccttgaag
     1741 caggcgggtt tgctgttgcc cttcctcgac aaaaaacacg gcgggcgcaa gggcagccag
     1801 tttgaaattc aggaagtctt gcggattgcg gggcattacg gcgtgcccgt tacgctgcgt
     1861 accggcatcg aaggcgcgct ggtgttgcag ccactgcaag agttcggcga tgaagcgcaa
     1921 atcgcacagg gtttggacat ggttttcaaa ggcgagggcg gtggtttggg cgttaccgaa
     1981 cccgaaacct ccggcgcggc gattgcccga gaaatgcagt cttactacga atatactgac
     2041 ggacaaacca tttacgtcaa cgccgcgaaa tactggcagg gcaactcgca aagcgacttc
     2101 ctcctcgttg ccgccaaaga acgcaaaaac ggcaaactcg ccaaagtcat cgacctgctg
     2161 ctcgtcccca aaacatacat ccgctgcgaa accctcgcat ccgaaggctt gcgcgccgtc
     2221 cgttacgccg tcaaccgcat cgatgccgaa atgcctgcaa ccgccgtgat gaaactctcc
     2281 cagagcgacg ctgccggttt gcgcgcgttc caaaacatct ttatccgcag ccgcctgcaa
     2341 ctgatcggca tgacgcacgg cattatggaa tacatccttg aaaacctgga acgatacgtc
     2401 cgcaacgaca tcagattcgt cgattacgaa cgtcgcgaaa tccaacgccg ccatcaggtt
     2461 tccgaaatcc tttaccgcta cgtctgccat tccgtttcgc ccgttgcacc cgtcgcccat
     2521 caactgatgg aggcaaacat catcaaaacc ctcgccacgg aatacactta cgccgccgcg
     2581 caaatgttgc aaaaactctt gggtgcgaag ggttttgaac gcggacacac cgccggcaat
     2641 atcgctatcg acatccgccc cttcacgatt tttgaaggcc cgaacgatat gctttatgcc
     2701 gaaatttacg accagttcgt ccgcgccacc gccgaagaaa aagaagcagg catgaagttg
     2761 gacaaaaacc aaaccctgct cgaccgcctg caaaccgatg cccgctttgc cgccgtcgcc
     2821 cgcgactaca ctttgcccga agacatccgc agcttcctgc aggaacacac cctgaccgat
     2881 gcctgcgccc tgcaaaaagt ctttatcggc aaaatcatcg cccgactctt tgtcttcgta
     2941 caggcggaac acgaagacgc ggcagcctgc ctgctgaacg acatccgcaa agacatattg
     3001 gactgccgat attgcggata gcggacaaat acggtggcag gcagacaatg ccgtctgaag
     3061 ggggaagtgc tttcagacgg catttcaggc ggcggatgcc gcatttcgcg ccgcgcccct
     3121 tgtgccgctt tcccacttac cctatttgtt cggaacatat ttatgaaaaa gagaaaaata
     3181 ctgccgctgg caatttgttt ggcggctttg tctgcctgta cggcgatgga ggcacgcccg
     3241 ccccgggcaa atgaagccca agccccccgc gcggatgaaa tgaaaaaaga aagccgcccc
     3301 gcgtttgacg cggcagccgt atttgacgcg gcagccgtac cggtatccga cagcgggttt
     3361 gccgccaatg caaatgtccg ccgttttgtg gacgatgaag tcgggaaagg ggatttttcc
     3421 cgggcggaat ggcaggattt ttttgacaaa gcggcttaca aggcggacat cgtcaagatt
     3481 atgcaccgcc cctccacatc gcgtccgtgg tatgtgttcc gcacgggaaa ttcgggcaag
     3541 gcgaaatttc gcggcgcgcg ccggttttat gcggaaaacc gcgcgcttat cgatgatgtg
     3601 gcgcaaaaat acggcgtgcc tgccgaactt atcgtggcgg ttatcgggat tgaaacgaat
     3661 tacggcaaaa atacgggcag tttccgtgtg gcggacgcat tggcgacctt aggctttgat
     3721 tacccccgcc gcgccgggtt tttccaaaaa gaattggtcg agcttttaaa gctggcaaaa
     3781 gaagaaggcg gcgatgtttt cgcctttaaa ggcagctatg cgggcgcaat ggggatgccg
     3841 caatttatgc cttcgagcta ccggaaatgg gcggtggatt atgacgggga cggacatcgg
     3901 gacatatggg gcaacatcgg cgatgtcgcg gcatcggttg ccaattatat gaagcagcac
     3961 ggttggcgca cgggcgggaa aatgctggtg tctgcaacat tggcgccggg tgcggatgtt
     4021 caggcaatca ttggcgaaaa aaccgccctg acgcggacgg tggcggattt gaaggcgtac
     4081 ggcatcatcc ccggcgaaga gcttgcagat gatgaaaagg cggttttgtt caaactggaa
     4141 accgcaccgg gcgtgtttga atattatttg ggcttgaaca atttttatac ggtatggcag
     4201 tacaaccaca gccggatgta tgtaacggcg gtcagggaca ttgccaattc gcttggcggc
     4261 ccgggattgt aatgtttttt aaaatgccgt ctgaaccacg tttcggttcg gacggcattt
     4321 ttgcatcctt ttatggggat ttcccgcatc agggcggtat tcggggcgaa tcccgcttca
     4381 gacggcattg ccggtcaagg cttgtccgaa ggaggatgtc cctgttcttt ggcgggcggg
     4441 aggaaaagta tgcagggatt gcttttaatc tgattccaaa aacttttaat cagattgcgg
     4501 atgctgccga ttttcgtgcc gcgcgagcgc agggcgacga agagggcgag gctgaagctg
     4561 acgcagaggt tgaccaggcc gatggcgagg acgctgaaaa tgccgagtac gaacgtgccc
     4621 aaaccgacgt tgccgctgac ggcggcatag ccgaggttgg cggaggaaaa ggcgacgtgg
     4681 cggatgtcca gcggcagccc gaggaggtgt ccgaaatagc cggtcatacc caagagcatc
     4741 ccgaagatga agttgccgac cagcgagccg tagtgtttgt ggatgtaggc ggcgaggacg
     4801 cggcggggcc cggggcgcat gattttacgc agcaaggggt tgaagggcag gcgttggcgc
     4861 aggttgaggt agtcggcgcg gttgtcgaaa aaacctgcga tgatgccgga gcagaacagc
     4921 cacagacctg caatggcggc ataccacagc gtcgggtaag cgatgatgtc tatggatttg
     4981 aactggtagg cggcggtgtg ggcatcgagt atgggcagcc ggtacagatg ggcatagccg
     5041 aacgatatgg cgcacgccaa aaggatggcg atggaaacgt tgccgaagac ggcgacactt
     5101 tgggagcggc atacgtcgat gaggagcttg gcgagtttgt tgtccaccgc tttgccgcct
     5161 tcgttgagat cgacctgttc ggcaaagctg gcggcagtca tcgcgggctg cttggtggcg
     5221 acggtgcagt gcagcatatg gatgatcata aagccgatgc cgtagttgaa cccagccgac
     5281 aaggaagtga ggaaggggct gaggccgagt gagccgatgc ggattttgag cagcgccatt
     5341 agggcgatga tgatgccgcc gcctgccgcc gagtagaaca ttttgaaata ttctttgcgg
     5401 ttgcgggtga tgtagtgttc gccgtggtgg cttttgtttt cggtaatgct gcgggcgagc
     5461 atacggatgc tgctgcggcg caggtggcgg gtgctgtatt gttcgaccgc cgcgtagatg
     5521 agggagttca tcagcgcgat ggtcaggcgg ttgctgttgc cggcgccggt ttggatgtcg
     5581 gtgagcagct tcagacggtc tacggtctgc cggagccgtt cgagcaggtg ggcgactttg
     5641 accgacgaac cggagccggc gcccgtccct ttgcggcgca aatagtcaat ctggctgaaa
     5701 cattggtcga acatcacttc gaggtgggcg gtgtcgtaag gcgtggtgcc gttgcggtag
     5761 tgttcgacca gtttggcggt ttcgcgttgg agggcgacga aggaagaatc ggcttccagc
     5821 aggcgcgggg cgatgcggat gaggtcgggt tcgatggctt cggatgccgt ccagacagac
     5881 agcatttcga tggcgcgcaa acgcgcatcg gcaaggcgga tgcccgccgt ctgcaatttg
     5941 gacggctcgg cgtggctccg gatgagttcg tataaaacca gccattggcg gatattgagc
     6001 gtttgcagcc atttgtcgtc gttttcggaa tggaaaagat aaaggaagac ttcgcccaag
     6061 ttggcaaaat ctttatatga cgggctgaag cgttcgtaga tgcgtatgcc catttcccgg
     6121 gcaaagctgt ggcgcgagaa gatgccgagt ttgatgagtg ccggataaat gtgtatttgc
     6181 gcgagccaaa tgtaaaaaca ccggctgaag cgggacagta attccctgtc ttgtttgaat
     6241 gtgtcgataa tcaggtcgaa acgttccccc gcacattttt tgccgccacg gcgcaaaaat
     6301 ttaatcagtg cgttgaggac gttgacaaaa tcggtatgtc ccaagctttc cgaaagcagg
     6361 gggcgcaggt tttgaggggt aattttcttc atatcagcca ttgtaccgca tcggcaatcc
     6421 ggcgttaaac gccccgatag cggggagggc gcgcctgcgg tgggcggcgc attttccgcc
     6481 ggtttgaaat caaactatat taatgatata ataaatatcg ataaatcaat ctgatagaat
     6541 aatccgtatt gatttttaat tttttcgatt ataacaattt cggccggttt gtgcatttcg
     6601 tcatccggaa gccggtattt taccggcagg ttttgaccgc tttgcccgat gaccgggttt
     6661 gtctgttttt aacaaaggag tgagaaggtt tgtccctgtc tgaatttata gaacgccgaa
     6721 cgtcatttaa tccgatggtt attttgacga ctttgttttt tgtgtgtgtt ttggtggtat
     6781 tggttttaac cgtgccggat caggtgcaga tgtggctcga tcgggcaaaa gaagtcattt
     6841 ttaccgagtt cagctggttt tatgttttaa cgttttccat ttttctgggt ttcctgctga
     6901 tactctcggt cagcagtttg ggaaacatca ggctcggacg ggatgaagat gtgccggaat
     6961 tcggcttcct gtcgtggctg gcgatgctgt ttgcggccgg gatgggcgtg ggtctgatgt
     7021 ttttcggcgt ggcagagccg ttgatgcatt atttttcgga cattacggcc ggcacgccgg
     7081 aacacaggca gcagcaggca ttgctgcaca cggtgttcca ttggggcgtt cacgcttggt
     7141 cggtgtacgg tacgattgca ttggctttgg cttatttcgg tttccgctac aagctgccgc
     7201 ttgccctgcg ttcttgtttt taccccctgt tgaaagaaaa aatttccgga aggttcggcg
     7261 atgccattga tattatggcg ttgcttgcta cttttttcgg catcatcacc acattggggt
     7321 tcggggcttc gcaactgggc gccggattgc aggaaatggg ctggattgcc gaaaacagct
     7381 tcagcgtgca ggttttgatt atcgccgccg tcatgtccct cgccgtcgtt tcggcaatat
     7441 ccggcgtggg gaagggcgtg aaggtgttga gcgagttgaa cctgggcctt gcgtttttgc
     7501 tgctgttttt tgttttggcg gcgggaccca ctgtttacct gttgtcggca ttcggcgaca
     7561 acatagggaa ctacctcgga aatctggtgc gcctcagttt taaaacttat gcgtacgaac
     7621 gggaacacaa gccgtggttt gaatcttgga cggtgcttta ttgggcgtgg tggtgttctt
     7681 gggcgccgtt tgtgggtttg tttatcgcgc gcatttcaaa ggggcgcacc atccgcgagt
     7741 ttgtcttcgg ggttttgctc atccccggcc tgttcggcgt tttgtggttt accgtcttcg
     7801 gcaatacggc gatttggctg aatgacgggg ttgcgggggg aatgctcgaa aagatgacct
     7861 cctctccgga aacgctgctt tttaaattct ttaattacct ccccctgccc gaattgacga
     7921 gcatcgtcag cctgctggtc atttctctgt tttttgtaac ttctgccgat tccgggattt
     7981 atgtcctgaa caatattacc tctcgggaca aaggcttgag cgcgccacgg tggcaggcgg
     8041 ttatgtgggg cgtgctgatg tctgccgttg ccgttttgct gatgcgctcg ggcggactcg
     8101 gcaacctgca gtctatgacc ctgattgttt ccctgccgtt tgccctgctg atgctgataa
     8161 tgtgtttcag cctgtggaaa ggcttgagtg cggataagaa atattttgag acccgggtta
     8221 accctaccag tgtattttgg acgggcggca agtggaaaga acggctggtg cagataatga
     8281 gccagacgca ggagcaggat attttaaaat tcctcaaaca taccgcatcg cccgctatgc
     8341 acgagttgca acgggagctt tcggaagaat acggcttgag cgtccgggtc gataagatgt
     8401 ttcatcagga cgagcccgca atcgagtttg tcattcgcaa agagacgatg cgcgatttta
     8461 tgtacgggat taagtctgtc gggcaggatg tatccgatca gttgattaac gacggcaact
     8521 tgccgcatat ccggcaccag acaacttaca aaccttacgc ttattttttc gacgggcgcg
     8581 tcgggtacga tgtgcagtat atgaacaagg acgagctgat tgccgacatt ttgaaaaact
     8641 acgaacgtta tttgatgttg ttggatgatg tcggtcagga actgatggcg cacgagcagg
     8701 tggaattggc agagtaagtg ccttatctcc ggcgtttgcc aaaaaaaatg cggtgaatca
     8761 gattcaccgc atttttttgc cggacggggc ttcggacggc acggcgttca tttgtttccg
     8821 tcaaactgct ccttcaacac ccgtttcaat accttgcccg tagcgttgcg cggcagcccg
     8881 tctttaaagt ggatttgttt ggggattttg aaatttgcca gcacggtacg caggtggcgg
     8941 cggatttcgt tctcgcccaa atccatacct tccttcaatt ggacgaaggc gacgatttcc
     9001 tcgtcggcat aacggtcttt cacgccgatg acggcagcgg cttcgacggc atcgagtttg
     9061 tagatttctt cttcgatttc gcgcggatag acgttttgac ctttggaaat aatcaaatct
     9121 tttttgcggt cgacgataaa gataaagccg tcttcgtcta tggtaacgaa atcgcccgtt
     9181 ttcaaccagc cgttgacgat ggtttcatcg gtggcggcag gcatattgag gtagccccgc
     9241 atcaccgaac cgcccctgac gatcagttcg cccacttcgc cgcgcggcac ttcgaccaat
     9301 tcttcatcga cggctttggc ttccaaaccg ggcaggggga tgccgacgct gcgggctttt
     9361 tgcctctcgg gcgtattgac ggcgacgacg ggagaggctt cgctcagtcc gtagccttcc
     9421 agcaatttgg cgcgggggaa cttggctttg aaatcgagga tggtttgttc cgccaaaggc
     9481 gcaccgccgc tgataaacag gcgaatgcgg ttgaaccatc tgaaatacca agggattttc
     9541 gccttgctca ttgcggtgta aatcgcgggt atgcccaaaa acacggtcgc gcgtttgagc
     9601 agggcctgtt tcaaaacgtt ggaaaacggg aaaacggatt tgaccaaaat aatcgaacac
     9661 gccatataaa tcggcagcag caccatagcc gtcagcgtga agctgtggaa catcggcagg
     9721 aaaacgataa agcggtcgcg cttggagatt ttaaagatgc gttcgatgcc gttcaggttg
     9781 gcgaacaggt tggcgtaact gattagcgcg cctttgggat gccccgtcgt gccggaggtg
     9841 tagatgatgt gtgccaaatc atttatccgg ggttggcggc ccaagtcggg tttttcgggg
     9901 aagcggcgca cgtcttcaaa aaaggcatcg ccttccgcca tttcgccggc cggacggctt
     9961 ttgtccgtcc aaatgatttt ttcgacgggc gtttgcgcct tcaagcccgc caactctttt
    10021 gacaggcccg ccgaggcgaa caggaagcgc gccttgcagt cgttcaggat atacgcgtat
    10081 tcgctgtttt tcaaaaatgt gttcatcggt acggcgaccg cgccgatggc ggagatggcg
    10141 aaataggcgg taataaattc tgtggaattg gaaaccgcca gcgcgaccgt gtcgccgaac
    10201 ttcacgccga tattttgcag atacgccgca acggcttcgg cttcctgctt gagcgcgcgg
    10261 taggcggttt tttccttgcc gtcgaacact gccgtgccgt tgccgttttt gcggcaggcg
    10321 gcggtcagca tttcgtagaa attggcataa gtccggttca tgaaacttcc tttatggttg
    10381 cggacggcga acgccctcat ttgaaaacaa tatgccgtct gaaaacggaa ggcggcagtt
    10441 tatcatcttt gccttcagac ggcatatcga acgccgcctt tgcgccgtat gccggaggtt
    10501 cgcctgcgtc tttccaatgt ctgaggcgaa tgccggttgc gtccggcaaa ccgtgcgcgg
    10561 ggtggctatg ccgtctgaag caatatgaag gtatgcgttt cagacggcat ccccctgttg
    10621 cggaaatacc gtccgcctcc tgtccgatta ttgcctgatt gcagcggcgc ggacgagttc
    10681 ttcgaggctg tcggcgagca tttccgccgc gcgccggtag cctttggcgg agaagtgtac
    10741 gccgtctttg gcggcccatc cttggttgag ccagtttttc atgctgcata tgccgcccat
    10801 ggcgttttgc caagaccaga acatggtttg tccctgccgg gcgacgcgcc gctgcatatg
    10861 ttggacttcg gtcaggcgga cggggcgtgt gccgcatacg ccgagcgtgt ttttcaggga
    10921 ttcgggtgcg ccgatgatga ggatgccggc ggcaggcagg ctgtcgtgga tttggcggac
    10981 ggtatccagc catttttgtt cagtgtcggc aatgtcgatg ttgtcgccga aggcttcgtt
    11041 ggtgccgtag gaaaggataa ccaaatcggc gccggtttgg gcaaggtcgt tcatacggtc
    11101 ggcacgccat ttcgaccaat gggtcaattg tgcgccgttg atgcccatgg cggaaacggt
    11161 aatgccgccg gcgggatttt cgatgttgat gaagccgatg tcccacggca tttcggtctg
    11221 tatggtcaag ggcagtgccg cgcgggtatc cagtacctgc cagccgccgc cgttggcgga
    11281 gacggtgttg ccgttgacgg tcagggtttg ttcggcgagc aggggtttgg taaacaggga
    11341 aacgcgctgt ttgccggtct tgccgtccga tgcggtcagg gtcatgctgc cgccgctgcc
    11401 ggtgtgggcg aggatgccgc cgagcgggaa gtctgctgct gcacttcggc tggtgaggct
    11461 ttgccagttg ccgttgtgcc ggacggccgc catgcgctgc cctttgacgt tggcggggta
    11521 gacccagcct atgccgccgt cgccccatgt tttttgcagg cgtttgcgga ggctgtcggt
    11581 aaagaagtcg ccggcggtat gcgagtcgcc gatttgcagg atgcggaagg tctcgccgct
    11641 gccttgtgcg acggattgga gttttttcat ccaaggcgag gcggcggcat tgccgtagtc
    11701 ggtcaagagc ccgtttgtat cggtgtaggg ggcggacggg gaaacggtaa cggtgtcgag
    11761 gctgacggag gcgacgggca gggcttctgc ctgcgtggcg gcgaacaggg cggaaaatgc
    11821 gatgaggtgt ttggaattca tggctgtgta cttgatggtt gcgtacttgg ttcaaaaacg
    11881 attttttcca ttatttttgc cgccagcagt ttttgtcctt cggcggtaaa gtgtatgccg
    11941 tccttgctgc ggtagcggac gggtttgccg ttgacgttga cggagtcggt gtggcggtct
    12001 tccccgccgc tcagggtgtg cgcggtggga atcaggatga ttttgccttc caggtgttcc
    12061 gaaagcagtt tgtcgaggta gcgcatctgc ccgtcgagct tggctttttt catgtagggg
    12121 atgccgagcc aaacgacttg gacgcggtgc gtgtgtgcgg cttcaaggat gcggtcgacg
    12181 cgtttcaggt attcttgcgc ccattcgtcg gaagcgaatt tgaggtagcg tttgccgacg
    12241 gggaaatccc acgggtcgtt cgggccgagg aagacggcga gcacgctgat ttcgggatgt
    12301 ttttgcaggg tttcttcaat cgttttcggc cagtcgaaga atgaggggta ggacagcccc
    12361 gtgctttgtt tgctgaggtt gacggattcg atgccgtatt gctgtttcag gcttttttgc
    12421 acgaaggggg caacgccctg catcagcgag tcgccgacaa aaaagacttt gtcgccgctg
    12481 cggacggcgg cggtgtcggt gtcctgtttc cattctgttt caccggtttg agggacggct
    12541 tcggatgtgg cggcttcaga cggcatatct gccgagccgc cgtcttgagc cgtcggcggc
    12601 gtttcgccgg acaggaatgt tttgatgccg tctgaaaagg cgtaggcgtt ttcttgcaac
    12661 gccgcaccgc tccgccacca tccgtaggcg gcgagcggtt cgagcgggct gttgcggtgg
    12721 taggtctgtt gccagtaggc gttgatgggg ttttggctga accacacggc aatcagggca
    12781 gacatcaata tggaggcgaa aagggaaaga aagtgtttca tgtccaaacc cttaaaaatt
    12841 ggcataaata aagccgggta tccccgaggg ggcgaggacg ataaccagca gcaggacggc
    12901 ggaaacgggg ataaaccaca gccacatcgg gattttttcc aaacctttga ccgcgtcgtc
    12961 gaaagcgcgt tgcaggtaag ggtagagcag catcacggat gcaaacgagg caagcaacag
    13021 catatccgcc tgttgcggcg cattccagcc gttggcattg gcaaagaggg cattgaaaac
    13081 tgcgcctgca tcgtcggggc ttgcggtatt gaagacgacg aaggtcaggc agacgaaatg
    13141 gaaagtgatg agccacgaga gcggcgcgag gtatttcaga cggcatagtg cgttgcgtcc
    13201 gaaatagcgg tcgcccgtgt tgagcagcac cagtgccgtg ccgtgcagcg tgccccaaat
    13261 gaggaagttc cagccgtagc cgtgccagat gcctgagagc accattgccg ccatcaggtt
    13321 gagctgtgtc cgcaaaaagc cttttttgct gccgcccaag gggatgtaga tgtagtcgcg
    13381 tatccaggtg gaaaggctga tgtgccattt gtcccaaaat gcacggatat tcgcggcacg
    13441 aagcggtgcg gagaagtttt tgggcagact aaagcccagc agcatcgcca taccgataac
    13501 caaatcggaa tatccggaaa agtctaaaaa gagttggaag gtatagccgt acacgccgcc
    13561 caatacgccc cagccgtcga attgggcggg attttcaaat acgggcgaca cccagttttc
    13621 cgccagcatc cccgccagcc accatttttt ggcaataccc agcagaatca gggaaacggc
    13681 gagtgcgggg cggacaggcg aacgtggatg gcgggtgcgg atttgcgcca gtgcgcccgc
    13741 ctgttcgccg tccgcgcttt tgaatgcggc ggcgcggata atcgggccgg aggtaacggt
    13801 ggggaaaaaa ctcaggtgca gtagcagttc gtgccactcg aaacgcgcgg cgtgcggcgc
    13861 acggaagcag taaaccaaat aggcgattga ctggaaggtg taatacgaaa gccccagcgg
    13921 catcaggatg tcgattgcgc cgccttgtcc ggcatattgt gcaatcagcg ggcggaaaaa
    13981 atcgaaatat ttgaaaaagc ccaagacggt cagcgagacg gcaatgccgc accccagcca
    14041 aaagcggcgc gtattttcgc gatcggaacg gagcagttcg cccaaaaggt acacgcagga
    14101 ggaataaagg acgacgattg ccgcaaatac ggggccgata tggtagagcc agcccatacc
    14161 ggcagccaaa agcagcaggt tttggacgga cgggtatttc gccaagcccc agtaaatcgg
    14221 caggaaggcg agaaagaaca gtgcgaactc gacagacagc agcggcataa accatccttg
    14281 gaataagaaa ataggcgtta aaacacggca aagcccgtta ttttaagcag gggcggatat
    14341 ttcttcaatc aaaatgccgt ctgaagggcg ggaggggctt cagacggcat cgcgcggtca
    14401 ggttcggata tggcggttca ggttcaggca gacccacaaa cctgccgccg ccgtcagcat
    14461 accggcgatg ccgatacggg aaatgccgac atattgcgtt acccaatgtc ccaacagtgc
    14521 gccgctgccg ataccgacgt t
//
LOCUS       DAWFJW010000048        14438 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFJW010000048 DAWFJW010000000
VERSION     DAWFJW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.048"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(230..553)
                     /locus_tag="WGR19_001141"
     CDS             complement(230..553)
                     /locus_tag="WGR19_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0798678.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAVEIDG
                     NMMRY"
     gene            complement(632..1288)
                     /locus_tag="WGR19_001142"
     CDS             complement(632..1288)
                     /locus_tag="WGR19_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0798679.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1390..1893)
                     /locus_tag="WGR19_001143"
     CDS             complement(1390..1893)
                     /locus_tag="WGR19_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0798680.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2077..2850
                     /locus_tag="WGR19_001144"
     CDS             2077..2850
                     /locus_tag="WGR19_001144"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0798681.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2857..3207
                     /gene="secG"
                     /locus_tag="WGR19_001145"
     CDS             2857..3207
                     /gene="secG"
                     /locus_tag="WGR19_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0798682.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3221..3306
                     /locus_tag="WGR19_001146"
     tRNA            3221..3306
                     /locus_tag="WGR19_001146"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3255..3257,aa:Leu,seq:gag)
     gene            4120..4488
                     /locus_tag="WGR19_001147"
     CDS             4120..4488
                     /locus_tag="WGR19_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0798683.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4485..4796
                     /locus_tag="WGR19_001148"
     CDS             4485..4796
                     /locus_tag="WGR19_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0798684.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(4765..4968)
                     /locus_tag="WGR19_001149"
     CDS             complement(4765..4968)
                     /locus_tag="WGR19_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798685.1"
                     /translation="MLSGYAVCTAAVSAWFDAVGHLCGYALIFGSAVLPVSSGIFSSA
                     SIVNLLILPFAKSSTQWLRCPCF"
     gene            complement(5194..6126)
                     /locus_tag="WGR19_001150"
     CDS             complement(5194..6126)
                     /locus_tag="WGR19_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0798686.1"
                     /translation="MLRCRPKSVLDSDGIFLKFNFLRSKPKYEISFLPSFLPSFKRIL
                     CLSAVISVLGACTVVADVYGQDSATMNAAAAEDYMKTVELNKSAGNVDTTSKTARRVQ
                     AVFRRMLPYADAANNTGHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDE
                     IAAIMGHEMTHALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILG
                     TYGITLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTH
                     PTNNARIENLKRLLPTVMPVYEQSVRNKGRVNPL"
     gene            complement(6149..6280)
                     /locus_tag="WGR19_001151"
     CDS             complement(6149..6280)
                     /locus_tag="WGR19_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ0798687.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIEGKPAKKIIAVPGRLANIVV"
     gene            complement(6410..7201)
                     /locus_tag="WGR19_001152"
     CDS             complement(6410..7201)
                     /locus_tag="WGR19_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0798688.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNIVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTSKAV"
     gene            complement(7429..10059)
                     /locus_tag="WGR19_001153"
     CDS             complement(7429..10059)
                     /locus_tag="WGR19_001153"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0798689.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVSVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVFAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGSQDGLSKELKDLRHKLHATIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPAVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10326..10841
                     /locus_tag="WGR19_001154"
     CDS             10326..10841
                     /locus_tag="WGR19_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0798690.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEGITTCQYKIIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10848..11291
                     /locus_tag="WGR19_001155"
     CDS             10848..11291
                     /locus_tag="WGR19_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0798691.1"
                     /translation="MPPRLLSGILCCLLTVSPVYAQGQPDAVNAYIQKKKVIVDTSKA
                     ELCFADDRQCYPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11460..13517
                     /gene="ppk1"
                     /locus_tag="WGR19_001156"
     CDS             11460..13517
                     /gene="ppk1"
                     /locus_tag="WGR19_001156"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0798692.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQTPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            13750..14403
                     /locus_tag="WGR19_001157"
     CDS             13750..14403
                     /locus_tag="WGR19_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0798693.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSDFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
BASE COUNT         3576 a         4056 c         3551 g         3255 t
ORIGIN      
        1 agcagcctgc acattgaaaa tgccgcccaa gcaaactttc agtttgccag cctcgtccta
       61 gccctctccc acggggagag gggattgggg tgcaggctgc ctttaaggtt caggcaaatt
      121 ttaacttcgt tgataccgcg ctttagcttc gcagaagctg cactttcaga cgaccttttg
      181 gaacaccaca ggtacacgca tttaaggaat gccgtctgaa atgcctgcct caataacgca
      241 tcatgttgcc gtcaatctca accgcccacg cgtcgatgcc gccctgaagg ttgtacaggt
      301 tttcaaaccc cgcgtccgcc agatacatcg ccgtatgcag gctgcggata ccgtgatggc
      361 aatacaccac aagcggcaca tcatccggca gctcgttctg ccgcagcgga atcagattca
      421 tcgggatatg cagcgcattt ggcagcgaac aaaccgccgt ttcttcatcc gtacgcacgt
      481 ccaacaaaca aaacatccgc ccttcgtcca tccacgcttt caattccgcg ggcccaagtt
      541 gcacaatatc catcgcaccc cccaaaaaaa accaagcaaa atgccgtctg aagccccaaa
      601 cccgctttca gacggcatcg caatccgaac atcaaaactg aaaaccgcca aacggatcgg
      661 catccttatc atccaaatgc gccaccaagg tatcgaacag caccttctct tcaaacacat
      721 cgcccttgcg cgtaatcaaa agcgcgcgct gaacaggctt gcggcctacg ataaccacca
      781 tgcgtccgcc atctttcaac tgttctttca acacttcagg cacaaggttt accgcaccgc
      841 cgacataaac cgcatcaaac ggcgcacctg cggaaagttc ggtcaacccg ttgttttgca
      901 cataatcgat attgtccaaa cccaagccgt ccaacaccgc tttggcgcgg ttttgctgtt
      961 cggcatcgat gtcgtccgac accacacgac cagccaattt tgccaacagc gcggtcgcat
     1021 agcccgaacc cgtgccgatt tccaaaaccg tatcgttttt cgtcagcttc aagccctgcg
     1081 ccagccgcgc cacgactttc ggctcgagca tcttatgacc gttggcaagc ggcagcgcca
     1141 tatccgcata cgccaaaccc tgcaagtcct catcgacaaa aagctcgcgc ggaatctccg
     1201 ccaaagcgtc caaaacgtca aaatccaata catcccaagg acggatttgc tgttcgacca
     1261 tattgaaccg cgctttttca aaatccattt agtgctccgt caaataattg tttcaacaat
     1321 ggcaggcatt ataaaaccac actcccgccg cgccaacttc ggcacgcgcc gcccgcccct
     1381 ttccctcact caagccgcct ggcgcgaagc ctcaaaccgc ttctccaaaa tcttcgccag
     1441 ttcgcccaaa tacaaagcat ctgtctcatc aaactgcgcc aaatgttcgc tgtccgcgtc
     1501 caacacgccg atacagcggc cgtctgaaaa cagcggcacg acaatctccg aacgcgacaa
     1561 agacgaacag gcaatatggt cgggatgcgc gtccacatcc ccgacgacca ccgtcccgcc
     1621 cttcgcccaa gcctgaccgc acaccccgcg accgaacgga atccgcgtac acgccaaagg
     1681 cccctgaaac ggtgccaaaa ccaattcgtc cgaacgcgta tcgaccaaat aaaaacccac
     1741 ccaaaaccaa ccgaacgcct ccttcaaaac cgccgccgtg ttcgccaaat tcgccaccca
     1801 atccgtctcg tccgccacca cagactcaat ctgcggcaac acctcccgat aaagcgcggc
     1861 cttgtccgaa gccgaaaaat gaagcgcgtg catcacatct cctatagttg catacatatc
     1921 aggcggccat tataaaacaa cctgcccgaa acaacattcc aaaccgcccc cgcagccgcc
     1981 cgccccaagt tgcgaacccg ccgcatatcc actaaacttc acattgcaac gcgccacacg
     2041 cggcagacag aaaaaacacg acacggagca aaaaagatgt atcaccaaat cggaatgtgg
     2101 gatcaaaaat gggtcatcgg caactggaaa atgaacggcc ggctccaaaa caacaattcg
     2161 ctcatgcacc gcttccgcat cctccccacc gccgaacgcg tcctcatcgg actcgccgcc
     2221 ccgaccgttt acctgctgca actgcacaac gccatgcaaa tcgtcttaaa caaccgcatc
     2281 ctcacctgcg cccaagacgt gagccgcttc cccgacaacg gcgcgtacac cggcgaagtg
     2341 tccgccgaaa tgctcgccga caccggcaca gacatcgtcc tcatcggaca ctccgaacgc
     2401 agcctttatt tcggcgaaaa aaacgaaatc caacgccgca aaatggaaaa cgtcctcaac
     2461 gtcggactca tcccgttatt gtgcgtcggc gaaagcctcg aagagcgcga agccggcaaa
     2521 gaacacgaag tcatcgccca tcagctttcc atcctgcaag ggctggatac caaaaacatc
     2581 gccgtcgcct acgaacccgt ctgggcgatc ggcaccggca aagtcgccac cgtcgaacag
     2641 attgccgata tgcacgcatt catctacaaa gaaatcttgt ctttgtgcgg aagcgatgtt
     2701 aaaatccgtg tcctttacgg cggaagtgtg aaagcggaca acgcggccga catcttcgca
     2761 gtaccttatg tggacggcgc actcgtcggc ggcgcgtcat tgtcgtacga ctcctttacc
     2821 gccatcatca gtgccgcaca aaatgcgtag aaaaatatgg aagccttcaa aaccctaatt
     2881 tggattgtta atataatttc cgctttggcc gtcatcgtgt tagtattgct ccaacacggc
     2941 aaaggcgcgg atgccggcgc gactttcgga tcgggaagcg gcagcgcgca aggcgtattc
     3001 ggctctgccg gcaacgctaa cttcctcagc cgctcgaccg ccgttgcagc aacatttttc
     3061 tttgcaacct gcatggctat ggtgtatatt cacacccaca cgacaaaaca cggtttggac
     3121 ttcagcaacg tacaacaaac tcagcaagca cccaaacccg taagcaatac cgaaccttct
     3181 gcccctgttc ctcagcagca gaaataacag tttttcaaat gccgacatgg tgaaattggt
     3241 agacacgcta tcttgagggg gtagtggccg taggctgtgc gagttcaaat ctcgctgtcg
     3301 gcaccaacac acaaaaacgc ctgaaaattt caggcgtttt ttgttcaatc ccatccaacg
     3361 ctgtcaacca attccaatca atacaaggat ttcaggctga ttgttatcct gccgtccccc
     3421 ttcctgacag tgcaatcccg tccaatccgc cctaattgaa ctaacctgat tttttacagc
     3481 atccgaaaat accgcgagaa aataccgtaa aaataaagct gaacgaccac caaatcagga
     3541 atgccaagcg ggaaatagct tgcggggaat actgacgcac cgtagggaat gagggggaaa
     3601 ccgtccaaga tgcagagcgg ttttttttgg gtttttggaa aaaacctata ctatagtgaa
     3661 ttaaatttaa accggtacgg cgttgcctcg ccttgccgta ccggtttaaa tttaattcac
     3721 tataaaataa gaaaaagata aaaaattggt aacaaatgcg gtaacaaatg cggtaacaaa
     3781 tggtaacgaa tcggtaacaa cttttggggt tttccggttt ttcaccgtct tggcagtggg
     3841 agcgtagcgg aatgaaaagc caaaacgcac ggaaccgcgc ctattttgag caggaatggc
     3901 ggttaaaccg cttggttata tacgggggga ataggaagac agcgaaacgc gctaaaaacg
     3961 caatttgagc cattaaaagc gattgattaa aaaataatca ggttagccgc cagtgtttca
     4021 ggcggcataa acggagaaat tgtggggcat aaaaaaggca gcttgccgtg ttgtctgtct
     4081 ctggtataat tccaagtatc actaatcaac ggctacacaa tgcggatatt caaaaaccaa
     4141 tggatagtga aatttgccaa gaaacataaa atcagtgatt ccgagctgct ggaagcggta
     4201 gagagggcgg ataacgggct gatagacgca gatttaggcg gcggtgtgat taagcagcgc
     4261 atagcaaggc aaggacaagg cagaagcggc ggctatcgca gcctgatatt gttcaaacag
     4321 gcagataggg catttttcgt ttacgccttt gccaagaacg acagagaaaa catttcggat
     4381 aaagaactgg acgtttaccg aaaagccgcc gcatattatc tgaaatacac gcgggcagag
     4441 ctggcggctt tgaaagaaga cggcattatc acggagatag aatcatgaaa tacaaaagcg
     4501 aggcattagc cgccattcat gaaatgatgg aaggggctta caacatcggc gcaatcgaca
     4561 aaaagactat gcgcggcttt gacaagtcct gcctgaccga aatcaaaccg ttgagcggcg
     4621 gagacatcaa ggcaatcagg gagaaggagg cactatcgca agccgctttc gccatctatc
     4681 tcaacgtggg aaaaaatcac gtttcggctt gggagcgggg cgttaaaaag cccagcggcg
     4741 cggcgttgaa gctgctgacc atcgtcaaaa acaagggcat cgaagccatt gcgtagacga
     4801 cttggcaaac ggcaaaatca gcaagttcac aatagacgcg ctgctgaata tgcctgacga
     4861 aacaggcaag accgccgaac cgaatatcag ggcgtagccg cacaaatgcc cgaccgcatc
     4921 aaaccaagcc gaaacggcgg cggtgcagac ggcatagccc gacagcaagg cacggcgcag
     4981 acgggcgtga aacccgaaac atccccgacc gcgaggcacg gagatttttt tgcgcccgtt
     5041 gcagggggga ttggatttaa gcggcgcggg cttgaaggca aaacgggcgg gcgcagaact
     5101 gtttaaatgc cgtctgaatc tcaaacgatt tcagacggca ttttgaaaca acggctcaaa
     5161 ttctcgatcc ccttccctta acgccgacgt tttttatagt ggattaacgc gccccttatt
     5221 tctgacactt tgctcataaa ccggcataac ggtcggcaac aaccgtttta gattttctat
     5281 acgggcattg tttgtcggat gagtagaggt aatagcataa ataaagccgt tttggtcgtt
     5341 ttcctgattc attttttccc aaaccctgac agcggccgcc ggatgatagc ctgcctgcgc
     5401 catcaacatc attcccccct catcggcttc ttcttccaag ctgcggctat aaggcaaggt
     5461 aataccgtac gttcccaaaa tatccatacc caatccgacc aattccggat tagtgtccgg
     5521 ttttttgtct aatataatct gcgtgcctat ctgcgccgcc gtattggtca aaatttgttg
     5581 cccgacctta tttttaccgt gttcatgcag ggcgtgcgtc atttcatgcc ccataatggc
     5641 ggcaatttcg tcatcggtca gcttgagttt gtcgactatc cccgtataaa acgccatttt
     5701 tccaccgggc attgcccacg cgttcagctc atcgtttttg aaaaccgtca ttttccagtc
     5761 aaacttatgg ccggtattat ttgccgcatc ggcataaggc aacatacgtc gaaatactgc
     5821 ctgcacccta cgggctgttt tggatgtagt atcgacattg ccggcagact tgttcaactc
     5881 aaccgttttc atataatctt cggcagccgc agcattcatt gtggcggaat cctgaccgta
     5941 aacatctgca acgaccgtac aagcccccaa taccgagatt actgccgaca ggcagagtat
     6001 ccgtttaaag gaaggaagga aggaaggaag gaaggaaatt tcatatttag gtttactcct
     6061 taaaaaatta aatttcaaaa aaataccgtc tgaatccaaa acggatttcg gacggcatct
     6121 taacattgtt taatgttttt aaaaagattt acaccacgat gttcgccagt ctgcccggta
     6181 cggcgatgat ttttttcgca ggcttgcctt ctatgaattt caccgcgcct tcagcggcgt
     6241 gttcggcagc ttcttcagcc aatttgtcga aattacgcat aaattgccaa taattccccg
     6301 acttttttac ggctgctgcc tttttcggca atattgcgct gaacttcaac tgttttcaga
     6361 atagcaaagg aataaatatc ccttgtgaat tcagtatcat gatttgaaat caaaccgcct
     6421 tggaagtcgg acgcaatttg tcgatttttt tgtaaagtcc gcgaccaatg aattcgatcg
     6481 tattttggtc gcgcaaaatt tgcaactgtt ggcggatttt gtctttgata tggttgtttt
     6541 ggggaaattg gatggatagt ttgttttcaa attcatacat ttgcgacaat gtgaattctt
     6601 cggggagttg gtcgatacat ttcataacag ccaaaagcca acctttgcgc tccgcatttt
     6661 ggttgcgtaa aaacagatta gattgccatt ttttcagaac ggtttcgggt tcgataatgc
     6721 gggaatcgtc tattaagaat atttttccgc tttcaggcaa aggggcgaga ttgatggaac
     6781 acataatgtg gtgcggccgg tttttaatgc ctttatttct gggaataatc atatccggcg
     6841 tgatgaaatg tttgggtaca agcaccaatt gccgtatgga gtaatccgct tttttatatg
     6901 caagaaagaa aaagttgggg ttggtatctg accggatgcg ctccaacatg gtgtgatatg
     6961 caccgtcagg cacgatgttg cctatggttt tttgattttt actctttaat tcatattgct
     7021 cgtggcaatt tgggcaaaag aggtctgcaa caggtttgtt attggcaaat ctcttcatcg
     7081 gcttgcttcc gcaacagggg cagtagccgt ttttttccaa ccaagcctcg ctcattacac
     7141 ggattttgtg ggttgcttta ttttgttgct tccccaattc agtatcgaaa aataaattca
     7201 tgttttggat tttgagatta agttttagtg gattaacaaa atcaggacaa agagacgaag
     7261 ccgcagacag cagggcaagg cggggcaacg ccgtaccggt ttaaagttaa tccactatat
     7321 ttgaggtctg tcatgcggac aacccaatct gccttaaaaa ggccgtctga aaccctgttt
     7381 ccaagtttca gacggccttt atccgtatgg ctaaacctta aaagcggctt agacgacgat
     7441 atttaccaag cgtccgggaa cgacgatgat tttcttcgcg ggcttgcctt ccatgaattt
     7501 caccgcgcct tcggtggcaa gggcggcggc ttcgaggtcg gccttggatg cgtcggcggc
     7561 gacggtgatt ttgccgcgca gtttgccgtt gacttgaacc atgacttcga tttcggattt
     7621 gaccaaggcg gcttcgtcga ctgccggcca gcctgcttcc cacagtttcg cgccgttcaa
     7681 ttcgctccac agggtttcgc agatgtgcgg cacgatgggc cacagcaggc gtacggcggt
     7741 ttccaatact tcttgggcga cggcgcggcc ttgttcgctg ccggtgtcgg ttttgtcgta
     7801 ttggttgagc agttccatca cggcggcaat ggcggtgttg aactgctgac ggcggccgta
     7861 gtcgtcgctg actttggcga tagtggcgtg cagtttgtgg cgcaggtctt tgagttcttt
     7921 agataaaccg tcttggctgc ctgcgaatgc tttgaccgcg ccgccttgtt tcaggtattc
     7981 gtaaacggtg cgccacaggc ggcgcaggaa gcggtgcgcg ccttcgacgc cgctgtcgct
     8041 ccattcgagg gactgttcgg gcggtgcggc gaacatcatg aacaggcggg cggtgtccgc
     8101 gccgtaggcg ttaatcagct cttgcggatc gacgccgttg tttttggatt tggacatttt
     8161 ttccgtgccg ctgatgacga cgggcagtcc gtcggctttg aggacggcga aaacagggcg
     8221 gcctttgtcg tcgaacgtca gctcgacatc ggcggggttg atccaatctt tgccgccttt
     8281 gtcgttttcg cggtagtagg tttcgcagac aaccatgcct tgcgtcagca ggcgttcgaa
     8341 cggttcgtcc acgctgacca agccttcgtc gcgcatcagt ttggtgaaga aacgcgcgta
     8401 caagaggtgc agaatcgcgt gttcgatacc gccgatgtat tggtcgaccg cgccccagta
     8461 tttcgcggcg gcaggatcga ccatgccgtc tgaaaatttt ggcgacatat agcggaagaa
     8521 ataccagctc gattccatga aggtgtccat ggtgtcggtt tcgcgtttcg ccgcgccgcc
     8581 gcagcatggg caggtggttt cgtaaaattc gggcattttt gccaaaggcg aacccatgcc
     8641 gtcgggtacg acgttttcag gcaaaacgac cggcagttgg tcggcgggga cgggtacgtc
     8701 gccgcatttt tcgcaatgga cgatgggaat cgggcagccc cagtagcgtt ggcgcgaaat
     8761 gccccagtcg cgcaggcggt attgggtttt cggttcgccc gcgccttggc tttgcagctt
     8821 ggcggcaacg gcatcaaatg ccgtctgaaa atccaagccg tccaaatcgc cgctgttgac
     8881 caatacgccg ttttctttgt cgccgtacca ctcttgccat tggttttcgt caaatgcgtt
     8941 gtcgccgacg gcaatgacct gttttttcgg caggctgtat ttggcggcga actcgaaatc
     9001 gcgttcgtcg tgcgccggaa ccgccattac cgcgccgtcg ccgtagcccc acaatacata
     9061 gttggcaatc cacacttcca gcttgtcgcc gttgagcggg ttgacgacgt agcggccggt
     9121 cggcacgcct tttttctcca tcgtcgccat atcagcttcg gcaaccgaac cggctttgca
     9181 ttcggcaata aatgcctgca attcgggttt gtcggcggct gcggcggttg ccagcggatg
     9241 ctcggcggca acggcaacat aagtcgcgcc catcagcgtg tcggggcggg tggtgtaaac
     9301 ttgcaggaat ttcgcgtaat cgccttccaa gccttgtttg ctgtcgtctg aaacggcgaa
     9361 gcgcacggtc ataccgcgag atttgccgat ccagttgcgc tgcatggttt tgacttgttc
     9421 aggccagtgt tccagtttgt ccaagtcgtt gagcagctct tcggcgtaat cggtgatttt
     9481 gaagtaatac atcgggattt cgcgtttttc gattaacgct cccgaacgcc agccgcgtcc
     9541 gtcgatgact tgctcgttgg caaggacggt ttggtcgacc gggtcccagt tcaccgtgcc
     9601 gtttttgcga tagacgatgc ctttttcaaa cagcttggta aacagccatt gttcccagcg
     9661 gtagtattcg ggtttgcagg ttgcggtttc gcgcgcccag tcaatcgcaa aacccaggct
     9721 tttgagctgg gttttcatgt attcgatgtt atcgtacgtc caagcggcag gggcgacgtt
     9781 gtttttcatc gccgcgtttt ccgccggcat gccgaacgcg tcccaaccca taggctgcat
     9841 gacgttgaag ccgtttaaaa gtttgaagcg gctcaataca tcgccgatgg tgtagttgcg
     9901 cacatgcccc atgtgcagct tgccgctggg ataggggaac atggagaggc aataatattt
     9961 gggtttggaa gcgtcttcgg agacgttgaa aatacgggcg tcgtcccatt ttttctgcgc
    10021 cgcaggctcg atggcggcgg gttggtaatg ttcttgcatg gtcattctgt ttttcgcttg
    10081 aaaacgttag gaatttaaag gaggaattat aacaggttgc cgatgtcggc gaatcggcaa
    10141 attgccggca ggatgcgtaa attcgcacgc gcatcattcc gtatgccgta caaatacacc
    10201 gcgtttactg atacgcacgt tttttatgct aatattacaa accaaaatca aatgtttaaa
    10261 actctcctga tgcggctctt ccgaacaaaa ggcagacggg catcgggtaa aagaggattc
    10321 tgcatatgaa aatcaaacaa atcgtcaaac cgggcttggc agtattggcg gcgggcgttc
    10381 tgtctgcctg cgcaaccaaa agcaacgtca aagccgacgg aacgaccgac aatccggttt
    10441 tcccgaaacc ctattccgta acgctcgaca acaatcgcgg tacattcccg acctatgacg
    10501 aattggactt gatgcgtccc ggtctgacca aagacgacat ctacaaaatc ctgggtcgtc
    10561 cgcattacga cgaaggtatg tacggcgtgc gcgaatggga ttatctgttc cacttccaca
    10621 ccccgggcgt aggcatcgac cctgaaaaca cttccggcgt agaaggcatt accacctgtc
    10681 aatacaaaat tattttcgat aaagacaaat ttgcccgcag cttctactgg aaccccgtct
    10741 tcccgaaaga tgccgcctgt ccgccgcccg cacccaaagc cgagccgcaa gtcatcatcc
    10801 gcgaaatcgt gccggcaaaa ccaaaacgta tccgccaata atacgcaatg ccgccccgcc
    10861 tgctttcagg gatattgtgc tgcctgctga cggtttcccc cgtatatgca caggggcagc
    10921 cggatgcggt caatgcctat atccaaaaga aaaaagtcat cgtcgataca tccaaagcgg
    10981 aactctgttt tgccgatgac aggcagtgct accccgtcct catcggcact gccacgccca
    11041 aggggacgtt cgggctgacg ctgaacagta ccgacaagcc cggatacggc ggcgaagtca
    11101 tcggcttcaa acaggagggc gattttcttt tcgccctgca ccgcgtttgg aatcagatac
    11161 cgtcggaaag gcggaacgaa cgcatcgcct ccccgtccgt gtccgacagg attatgacca
    11221 acggctgcat caacgtcagc gatgcggtgt acgaaaaact gcgtcattat tttgtgttgg
    11281 aagtgatttg aaacagacgg ataccgcacg cgccggtatc tgttttcaca ttgccccgat
    11341 gcctgaaaca gactgtccgc cacgtcatgc cgtctgaaac cggcgcagat gccgccaagc
    11401 cttcagacgg cattgcctgc ccgctccgac cgaacaacaa ccatctttgg gagaacctta
    11461 tgcccgaaca aaaccgcatc ctctgccgcg aactgagcct gctggcattc aaccgccgcg
    11521 tgttggcaca ggcggaagac aaaaacgtcc cccttttgga acgcctgcgc ttcctgtgca
    11581 tcgtgtcgtc caatcttgac gagtttttcg aagtccgcat ggcgtggctc aagcgtgaaa
    11641 acaagctgca cccccggcgc aggctggaca acggcaaaat gccgtctgaa actatcgccg
    11701 acgttaccga agcggcgcgc tccctgatac ggcaccagta cgacctgttc aacaacgtcc
    11761 ttcagcccga gttggcgcgg gaaagcatcc atttttaccg ccgccgcaac tggacgggcg
    11821 cgcagaaaaa atggatcgaa gactatttcg accgcgaact gctgccgatc ctgaccccca
    11881 tcggactcga cccgtcccac cccttcccgc gaccgctgaa caaatcgctc aacttcgccg
    11941 tcgaactcga cggcacggac gcattcggca ggccttcggg gatggcgatt gtgcagacac
    12001 cgcgcatcct gccgcgcgtc gttcccctgc cgtccgaact gtgtggcggc ggacacggct
    12061 tcgtcttcct gtcgtccatc ctccacgccc acgtcggcaa actcttcccg ggcatgaacg
    12121 tcaaaggctg ccaccagttc cgcctgacgc gcgacagcga cttgaccgtt gacgaagaag
    12181 acctgcaaaa cctccgcgcc gccatccaaa acgaactgca cgaccgcgaa tacggcgacg
    12241 gcgtgcggct cgaagtcgcc gacacctgtc ccgcctacat ccgcgacttt ctgctcgcgc
    12301 aattcaaact gaccgccgcc gaactctatc aggtcaaagg cccggtcaac ctcgtgcgcc
    12361 tcaacgccgt ccccgaccta gtcaaccgcc ccgatttgaa atttcccaca cacacgccgg
    12421 gcagactgaa agccttgggc aaaaccgcgt ccatattcga tttggtgcgc caatcgccca
    12481 tcctgctgca ccacccctac caatcgttcg accccgttgt cgaaatgatg cgcgaagctg
    12541 ccgccgaccc cgacgtgctt gccgtcaaaa tgacgattta ccgcaccggc acgcgttccg
    12601 aactcgtccg cgccctgatg aaggcggcac tcgccggcaa acaagtaacc gtcgtcgtcg
    12661 aactgatggc gcgttttgac gaagccaaca acgtcaactg ggcgaagcag ctcgaagagg
    12721 cgggcgcgca cgtcgtgtac ggcgtgttcg gctacaaagt ccacgccaag atggcactgg
    12781 tcatccgccg cgaagacggc gtactcaagc gttacgccca cctcggcacg ggtaactacc
    12841 accaaggcac atcgcgcatc tacaccgact tcggcatcat taccgccgac gaacaaatca
    12901 ccgccgatgt gaacacattg tttatggaaa tcacaggctt gggcaaaccc gggcggctga
    12961 acaaactcta ccaaagcccg tttaccctgc acaaaatggt tatcgaccgc atcgcacgcg
    13021 aaaccgaaca cgcaaaagcc ggcaaaccgg cgcggattac cgccaagatg aattcgctca
    13081 tcgaaccgac cgtcatcgaa gccctgtatc gggcaagcgc ggcaggcgta caaatcgatt
    13141 tgattgtgcg cggtatgtgc accttgcgcc cgggtgtaaa aggcttgtcc gaaaacatcc
    13201 gcgtccgctc catcatcggc aggcagctcg aacacgcgcg cgtgtattgc ttccacaaca
    13261 acggcgcgga cgataccttt atctccagcg cggattggat ggggcgcaac ttcttccgcc
    13321 gcatcgaaac cgccgcgccg attaccgcgc ccgaactcaa aaaacgcgtc atccgcgaag
    13381 gtttggaaat ggcattggca gacaatacgc acgcgtggct gatgcagccc gacggcggct
    13441 atatccgcgc cgcacctgcc gagggcgaat ccgaagccga cctgcaaaac gatttgtgga
    13501 cactgctcgg aggctgaccc gcaccgcccc aatcaaaaac catgccgtct gaaacctttc
    13561 cgtttcagac ggcatggttt tacagcaatc taaacagggc ggaccggagt caaaaacaca
    13621 ccttcgccat ttccgcacaa gcacttcccc tatacgctcc caaccccaat ccgccgcatt
    13681 ccagacggca ttatagtgga ttaaatttta ggggctgtac tagattagca gatatgttac
    13741 cctcgaaata tgaagataac acactgcaaa ttaaagaaaa aagtacagaa agaactgctc
    13801 cgtttttttg tgctggaagt taccgcccgt tctgccgccg atattttggg tatccatccc
    13861 aattcggcag cactgttcta ccgtaaaatc cgcacggtta tcaaccatca tttggccttg
    13921 gctgccgatg aggtttttga gggccctgtc gagccggacg aaagcgattt cggcggacgg
    13981 cgtaaaggca gacgtggtcg cggtgcggca ggaaaagtgg ttgtcttcgg cattctgaaa
    14041 cgcaacggac gggtctatac cgttgtcgta aacaatgcca agtctgaaac gttactccct
    14101 gtcatcaaga agaaaatcat gccggacagc attgtttata ccgatagtct gagcagctac
    14161 gacaagttgg acgtgagcga tttcattcat taccgcatca accattccaa ggaatttgca
    14221 gaccgtcaga accacattaa cggcattgag aacttttgga atcaggcaaa acgcgtcttg
    14281 cgaaaataca acggaatcga tcgtaaatct ttcccgctgt tcttgaaaga atgcgaattt
    14341 cgatttaact tcggcacacc gtctcaacag cttaaaatcc tgcgggattg gtgtgggatt
    14401 tagggctaat ctagtacagc ccctaaattt aatccact
//
LOCUS       DAWFJW010000049        14316 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFJW010000049 DAWFJW010000000
VERSION     DAWFJW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.049"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(140..568)
                     /locus_tag="WGR19_001158"
     CDS             complement(140..568)
                     /locus_tag="WGR19_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0798694.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLGYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(633..2381)
                     /locus_tag="WGR19_001159"
     CDS             complement(633..2381)
                     /locus_tag="WGR19_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0798695.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2708..4141
                     /gene="ccoN"
                     /locus_tag="WGR19_001160"
     CDS             2708..4141
                     /gene="ccoN"
                     /locus_tag="WGR19_001160"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0798696.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4168..4779
                     /gene="ccoO"
                     /locus_tag="WGR19_001161"
     CDS             4168..4779
                     /gene="ccoO"
                     /locus_tag="WGR19_001161"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0798697.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4784..4954
                     /locus_tag="WGR19_001162"
     CDS             4784..4954
                     /locus_tag="WGR19_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0798698.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4978..6075
                     /gene="ccoP"
                     /locus_tag="WGR19_001163"
     CDS             4978..6075
                     /gene="ccoP"
                     /locus_tag="WGR19_001163"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0798699.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6115..6309
                     /locus_tag="WGR19_001164"
     CDS             6115..6309
                     /locus_tag="WGR19_001164"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0798700.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAIDYMVNQSGGKF"
     gene            6511..7920
                     /locus_tag="WGR19_001165"
     CDS             6511..7920
                     /locus_tag="WGR19_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0798701.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            8022..11231
                     /gene="recC"
                     /locus_tag="WGR19_001166"
     CDS             8022..11231
                     /gene="recC"
                     /locus_tag="WGR19_001166"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0798702.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLALLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLTDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11301..12869)
                     /gene="mtrF"
                     /locus_tag="WGR19_001167"
     CDS             complement(11301..12869)
                     /gene="mtrF"
                     /locus_tag="WGR19_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0798703.1"
                     /translation="MNQTDTRRGGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13201..13413
                     /locus_tag="WGR19_001168"
     CDS             13201..13413
                     /locus_tag="WGR19_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798704.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYENIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFNQENVHAMIEEIKNAI"
     gene            complement(13506..14129)
                     /gene="mtrR"
                     /locus_tag="WGR19_001169"
     CDS             complement(13506..14129)
                     /gene="mtrR"
                     /locus_tag="WGR19_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0798705.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEGGSWAVFRHTLLHFFERLQSNDIY
                     YKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKETAV
                     IFIKSTLDGLIWRWFSSCERFDLDKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT         3565 a         3427 c         3789 g         3535 t
ORIGIN      
        1 gtcaaatttc gttaacagac tatttttgca aaggtctccc actgtaaaaa cggagaagga
       61 cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt tgcggtggaa tgccgcttga
      121 aggtgttttc ggcttgtaat cagacgcggg cgttgtccag ccgctctctg aagttttcca
      181 aatttacgcc tgccgcttgg gcgcggcgga tgatttcgtc aaccggcata ccttctgccg
      241 cccggcggaa gccccacagg agggagcagc gcgtaccggt ccggcaatag ccaaggacgg
      301 gatattcggc ttgtccgatg agttggcgga aggtttcgac atcgtgtttt tggatgtcgc
      361 gtgcggtaac gggttggtga tggaatccag taacgcctgc ttgttccagc cactgtttga
      421 tttgggcgaa gtcgggttgc gattcttctt cgcggtcggg gcggttgcag atgacggttt
      481 tgatgcccag ttgcgcgatt tgttccgcat cggctttggt cagttgcgga gaaatataga
      541 ggtgttcgtc aagtttcaga atggccattt tgtttccttt actttgcggt tgtttttcag
      601 acggcatttt gtgtgcaatg ccgtctgaaa gtttaaccga acaaccagtt gtccaacagg
      661 ttccggtctg tgatatgtag gatggcagta tccgctgccg ctgcctgaac cgtcaagacg
      721 gtttgacgca atatgccggc acggaagaag tggatattga tttttgcatt gacgtggtat
      781 cgggcccatt gtgcggtaaa gtcggtgcag gcataaccgt ctaaagcaat gattttgtct
      841 tgcgggcaca gtgccgcaga ttccgcgctg ccgccgttga agacatgggt caggacgatg
      901 tggtcggtgt tttgtttgaa acgtgcgcca aaatcgcctg ccgacgggac ggggcagatg
      961 tgttctgcgt atccgccgcc gtgttgtcgg ggaagcggca ggaaggtcag tcccacgcct
     1021 gcggttgcca ggcattcggc aagcggcaaa tcttcggtac tgtataacgc tttttggaaa
     1081 aaatcttcca aatccaagcc ggtaatttcc tgacagcgga tttgccagtg tttttccgga
     1141 atacccgagt gtgtgtccct ccactcccga tagagtttgt ccattaacgt atcgagagaa
     1201 tgtctgccgt tgcttcggtt gcgtattatc agatcaaggc acaatgcggc aagcgcgcct
     1261 ttctggtagt agctgacgat ggcgttgggg ctgttttcat ccggtttgta aaatttgttc
     1321 cacgcggtaa aactcgattc cgccaaggtc tgcctcaaac ggccgcgggt ttgttgtacg
     1381 cgcgtaatgc cttgtgccag caggtttaaa taagattcgg gcgagatggt gcggctgcgt
     1441 gccaaaaaca aatcgtcgta ataggatgta ataccttcga atgcccatag ttgttcggta
     1501 tagttttctt tgtcgaggtc ataagggacg aacgcggcag gtttgatgga tttgacgttc
     1561 cacgcgtgaa aatattcgtg ggagaaaagt ccgagcaatg tggtgtaggt atcgtcggca
     1621 tcggtcatac cgtacggcgg aaggctgtgg cggtcggcga gcagggcggt gctgtcggtg
     1681 tgttccaaac cgccgtaaat atggtcgccg acgtggagca ggaacaaata tttttgaaac
     1741 ggggcagggg aggaaaacac cgccagttct gtttcgcaga tttttttgat atccgaaacc
     1801 agcctgttgc ggtcgaaatc gggatatatg ccgcttaagg caattgtgtg cggaatgcct
     1861 gccgcctcaa aatctaaaaa ttcaatcaag cccatctcga caggtcggtc aatcaattcg
     1921 gcataagatg ccgcctgaaa gacaaacctc cctgtttccg gcagcgttgt ggcaatacgc
     1981 cattcggacg gaatacccgt caattccaag cggtgcggca gcgtttccgt tccttcgact
     2041 ttcaaaaaca ggcacgatcc gtcaaaaaaa ccgcgttctg tcgtcaggaa agaacctcga
     2101 accgacaaat cgaatgcata tacggtgtag cggatttgcc actcgccgcg tacggcggca
     2161 gcgtgccagc ggttttttgc aatttgttcg agcggcatgg acgtgccgtt acaggatgca
     2221 tggatagaag tgatgtggcg ggaaaaatcc cgaatcagat agctgcccgg tacccaattc
     2281 ggcaggctta tttctatagg aagatcatca tcttgtgtga atgtcagcag gatgtgccac
     2341 tcgtggtcga gaggggacgg ggttatttta taatgaatca taactatcaa atatatcgtt
     2401 aaacgggatg gattaaatac ctttaaaatg cagcttaacc tacatcaata aagtgccaca
     2461 aaaagggaaa attggaaaca aaagcaatgg cggtcgggtt gtaatttatt ataacaatat
     2521 gatttatatg taataaaaaa cagattaagc aaagagctcg ccggcggcgc gaatgtaatt
     2581 ccgcttggcg taataaggca gatatgttaa atttagaaaa aattacgctt gcccggattg
     2641 caccttaaac aggctgcgga agcggcagga taaaaatctt tattcccata aattcatgga
     2701 gactctcatg gacacacaaa cttacaacta caaagtggtg cgccagttcg ccatcatgac
     2761 tgtagtttgg gggattgtgg gtatgttggt cggcgttatc gtcgccgccc agctttttgc
     2821 tcctgccctc gacttgtcta atatcggacc ttggttccac ttcggccgcc tgcgtccgct
     2881 gcacaccaat gcggttattt tcgcatttgg cggttgcggc ctgatcggca catcatacta
     2941 cgttgtccaa cgtacttgta atacccgtct tttcggcggt tggctgccgg catttacctt
     3001 ttggggctgg caggcggtaa tcgttgccgc cgtcgtcagc ttccctatgg gttggaccca
     3061 aggtaaggaa tatgccgaac tggaatggcc gatcgatatt ctgattactt tggtttgggt
     3121 ggcttacgcc atcgtattct tcggtacgat tgccaaacgt aagattaaac atatttacgt
     3181 tgccaactgg ttctacggcg gctttatttt ggcggtcgca cttttacaca tcgtcaacaa
     3241 tatcagcatc ccggcagggt tgatgaagtc ataccccgtc tattccggtg cgattgatgc
     3301 tatggttcaa tggtggtacg ggcataacgc cgtgggcttc ttcctgactg ccggcttctt
     3361 gggtatgatg tactatttcg tacccaaaca agcagcccgc cccgtttact cctaccgcct
     3421 gtccgtcgtt cacttctggg cgttgatttt cacctatatg tgggcgggtc cgcaccatct
     3481 tcactacact gcgctgcctg actggacgca atctttgggt atggttctgt ctttgattct
     3541 gttcgcaccc tcttggggcg gtatgattaa cggcatcatg accttgtccg gcgcgtggga
     3601 caaactgcgt acagacccga ttcttaaatt cctgattgta tccttgtcct tctacggtat
     3661 gtctaccttt gaaggcccga tgatgtcgat taaaacggtc aatgcattga gccactatac
     3721 ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc tgggtaggct ttgtaaccat
     3781 cggttccgtc tattacatga ttccccgtct gttcggcaaa gaacagatgc acagcaccaa
     3841 gctggtagaa gcacattttt ggattgcgac catcggcgtg gttctgtata tcgctgccat
     3901 gtggattgcc ggtgtgatgc agggtttgat gtggagttct ttgaacgatg acggtacgct
     3961 gacctactcg tttgtcgaat ccgtaaaacg caccatgcct tactacgtga tccgttttgc
     4021 aggtggttta ttgtatctga gtggtatgtg cattatggcg tacaacgtgt accgcacagc
     4081 catcggtggt aaagcagtcg atgccgaaat ccctgcggtt tcccaaacac agcaccacta
     4141 aaatactaag aaagataggc taccaaaatg aaattacaac aattggctga agaaaaaatc
     4201 ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg gtctgttgat tgaagttgtg
     4261 cccttggcct ttaccaaggc ggcaacacag ccggcgccgg gcgtgaagcc ttacaatgcc
     4321 ctgcaggttg ccggacgcga tatttacatc cgtgagggct gttacaactg ccactcgcaa
     4381 atgattcgtc cgttccgtgc ggaaaccgag cgttacggtc attactctgt tgccggagag
     4441 tcggtttacg accatccgtt ccaatggggt tccaaacgta ccggtcctga tttggcacgt
     4501 gtgggcggtc gctattccga cgaatggcac cgtatccacc tgctgaatcc ccgtgatgtc
     4561 gtgcctgagt ccaatatgcc ggcattcccg tggcttgcac gcaataaagt cgatgtcgat
     4621 gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta ctccttacag tgatgaggaa
     4681 attgcgaaag cacctgaggc tttggcaaac aaatccgagc tggatgctgt agtcgcctat
     4741 ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac atcatggata ttaacggtat
     4801 tcgtgctctc ttcacggtat ggatctttat ctgtttcctg ttggtactct atatcgtctt
     4861 caacaggcgg aataagaaaa actacgataa tgccgcaaac agcatttttg atgaaaacca
     4921 agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt gataacggag caaaacaatg
     4981 aacacaacat cccaatttac cagtaatttc tggaatatat atattgcagt tattgtctta
     5041 ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa atgttgtcaa acgtccgaag
     5101 aagggcgaag aagtacaaac tacgggtcat gagtgggacg gcattgccga atacgacaat
     5161 ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt ggctgttcgg tatcggttat
     5221 ctggttatgt atccgggtgt cggcgactac aaaggtctgc tgaaatggac cagccataac
     5281 caatatgaaa aagaggtcaa aaaagccgat gagcaatacg gcaaactgta tgccaagttt
     5341 gcggatatgc cgattgaaaa agtggcaaaa gaccctcaag ccaagcaaat cgcccaaaac
     5401 ctgtttaaca cttattgtat ccagtgccac ggctctgatg ctaaaggctc taaaggtttt
     5461 ccgaatctga ccgatagcga ttggttgtgg ggcggtgatc ccgataaaat tcacgagacc
     5521 atcgaaaaag gccgtgttgc gactatgcct gcctggggtc ctgctttggg cgaagaaggc
     5581 gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac ccgaaggtca gtatgatgag
     5641 gaacgtgccg cgcgcggaca agccttgttc agcggtccgc ctgccaactg tttcacttgc
     5701 cacggcgata agggacaagg tatccaaggt ttgggtccga acctgactga tgacgtgtgg
     5761 ttgtggggcg gtacgcaaaa atccattatc gaaaccatta ccaacggccg cagcagccaa
     5821 atgcccgctt ggggacattt cttggataaa gacaaactgc atattatgac tgcttatgta
     5881 tggggtcttt ccgataaaga cggtaaagct ccggtgaaaa aagccgagcc tgcaccgact
     5941 cccgcaccgg cggcagaacc cgcagcctct gctcctgcag aagcagcaca agccgcgtcc
     6001 gaagccaaac ctgccgcagc agaacctaaa gccgaggaaa aagccgcacc tgctgccaaa
     6061 gcggacggca aataggttta tgaaaccgtt tgtgccgcct gccatggcaa tgcgattccg
     6121 ggtattcccc atgtaggcat caaagccgat tgggccgacc gcatcaaaaa aggcaaggac
     6181 acgttgcaca aacacgcgat cgaaggtttc aatacgatgc ccgcaaaagg cggtcgcggc
     6241 gatttgagcg atgatgaggt taaagctgcg attgactata tggtcaacca gtcaggcggc
     6301 aaattctaac ttgactgagt ttccgatatt atcggttatg aaaatccaga ttcagtattg
     6361 aatctggatt ttttgtttta acgggcatcc ggttttaaac gcagactgcc tatttgttgt
     6421 aatgaatttt gactttagtc ataaagaatt agtctttatt gtttataatc gcgcatcatc
     6481 acgatagccc tattttcaaa aggacgatta atggatacac aaatcaaaac tgaagccggt
     6541 cagcagaata accggcgtta tctgaccgtt tggcggtggc atttttatgc cggtctgctg
     6601 gttatgcctt ttcttaccct gcttgccgtt acgggtttgg gtatgctgct gtttgccaat
     6661 attaccggta aggagggcga gcggattcat gttgtgccgc aggcaacggt acaacctctg
     6721 tctgttcagg cggaagcggc acgcagtgcc gttaatccgg aaacttcgtc cgtcgttcag
     6781 tatattgcgc cgcgtgccga tgatatggtt gccgtgttcc gtgtcaacaa tgagggcaag
     6841 gcaacgatgg tcgcggttga tccttatacg gcaaaagtgg tcagtaccat gccgcgcaat
     6901 cagggttggt attacgcgat ggatgaaatc cacagcgata tgatgctcgg ttcgacaggt
     6961 gattatcttt tggaaacggc tgcatcgctg acgattatca tgataatcag cggtttgtac
     7021 ctttggtggg tgaaacggcg cggcatcaag gcgatgctgc tgccgccaaa aggcagggcg
     7081 cgttcttggt ggcggaatct gcacggcgcg tttggaactt gggtgtcgtt gattttactg
     7141 ttgttctgcc tgtcgggtat tgcttgggca ggtatttggg gcggcaagtt cgtgcaggct
     7201 tggagtcagt tcccggccgg caaatggggt gtcgaaccga accctgtttc agtcgtgccg
     7261 acccacggcg aggtattgaa tgacggcaag gttaaggaag tgccgtgggt tttggagctt
     7321 acgcctatgc ctgtttcagg gacgactgtg ggcaaagacg gtattaaccc tgacgagccg
     7381 atgacattgg aaaccgtcga ccgttttgcg cgggaaatcg gtttcaaagg gcgttatcag
     7441 ttgaatttgc ccaaaggcga ggacggagtg tggactttgt cccaagactc tatgagttac
     7501 gatatgatta gtccgtttgc cgaccgcacg gtgcatatcg accagtacag cggcaaaatc
     7561 cttgccgaca tccgctttga cgattacaac ccgttcggca aatttatggc ggcaagcatt
     7621 gcgctgcata tgggtacttt gggctggtgg agcgtgttgg cgaacgtttt gttctgcctt
     7681 gccgtcattt ttatcggtat cagcggctgc gtgatgtggt ggaaacgccg tccgaccgga
     7741 gcggtgggca tcgttccgcc ggcgcagaaa gtcaagctgc cggtttggtg gatgatggca
     7801 ttgccgctat tggcaatcgc actgctcttc ccgaccgcac tgcttgccat tgccgtgatt
     7861 tggctgttgg ataccttgct gctgtcgcgg attcctgttt tgaggagatg gtttaaatga
     7921 cccgatgccg tctgaagcgt tcagacggca ttttgtttga aggcgggcgg gggaaaggct
     7981 atataatccc gaatacttga ccacagcttc tgtttgaaat catgttttat ctgtatcaat
     8041 ccaaccgtct tgaaacgctg gcggcactgt ttgcccgcat tcaaaaagtc aaaccgctga
     8101 aatcggtttt acagcccgaa cagattattg tgcagagtca ggggatgcgc cgctacctca
     8161 atacctgcct cgcccgcgat ttgggcgtgg cggcgaattt gtcgttcagc ctgcccgccg
     8221 gtctgacgtg gaagctgatg aaaaaactga ttcccggtat tccggaactc agcccgtttg
     8281 cgcctgaagt gatgcgctgg cggctgttgg atttgttccg cagcgaggca tttcggaata
     8341 cggcagaatt tgaagatgtg aggaatgtgc tgcaagacta tctaggcagc ggcgaatcgg
     8401 cagattatca gcttgcggga cagcttgcgg acatattcga ccaatacctt gtctatcgtc
     8461 cgcagtggat agacgcgtgg cagcagggca ggatactcag tttgggcgac gatgaaatct
     8521 ggcagtccaa actgtggcgt tatcttgatg acggcaggca gagcgcgccg caccgtgttg
     8581 cgttgtggga aaagctgttg gaatctttga gcagtgataa gctgcccgag cgttatttcg
     8641 tgttcggcat ttccactatg gcgccgatgt atttgcagct tttgcacaag ctgtccgaac
     8701 attgcgatgt gttcgtgttt gcactcaatc cgagcgggat gtattggggc aacgtcatcg
     8761 aagcggcgca aatcctcaaa ggcggcggcg atcccgattt aactcaggca gggcatccgc
     8821 tgctcgcctc attgggcaag caggggcgcg actttttcga ctttttgaac gaaatggaaa
     8881 tagaaggcga aacgccggta tttgaagaag gcggacgcga tacgcttttg cacgccctgc
     8941 aaaccgatat tcaaaacctg aaaatgccgt ctgaaaatgt gggaagcgtc aacacgggcg
     9001 acggctcgat acgcatcgtc tccgcacaca gccccttgcg cgaattgcag atactcaaag
     9061 acaagctgtt gaaaattctg catgaacatc ccgattggca gccgcatgat atcgccgtat
     9121 taaccccgaa catcgaatcc tatacgcctt ttatcgaagc cgtgttcgga caggcgcagc
     9181 ccggtgcgca ggcattgccg tattccgttt cagacgtgaa aatcagccgc cgacaaccgt
     9241 ttttttatac attggaatgc ctgtccgact tgttggaaag ccgatttgaa gtcgataaag
     9301 tgctcgcgct tttggaaacc gcgcccgtat tgcgccgttt cggactgact gaggacgatt
     9361 tgccgctttt gcacgatatg gttgccgatt tgaacgttca ctggggtttg gacggagaaa
     9421 tgcgcggcgg cacggatcag ctgttcacct ggaagcaggc ggtagaacgc atgatattgg
     9481 gctggatgct gcccgaaggc ggcaacccga tgtggcagga tgtcagcgcg tggtatgccg
     9541 acgtgaacca aaccgccctg ttcggacgtt ttgccgcctt cctcgaaacc ctgacggata
     9601 ttgtacggat atggcggcag cccgcaacga tcggcgaatg ggtggcgcgt tgccgggatt
     9661 tgcttgaaac attgttccaa gctgaagccg atgaccaaaa gtcagtccaa aaccttgaaa
     9721 acgaatgggt caaatggcag gcggaaacag aattggcgca attttccgga cagttgccgc
     9781 aacaaaccgt catccgccat atccgacgtt tcctcgacag cgaaagcgag gcaggctttt
     9841 tacgcggcgg catcaccttt tgcagtatgg tgccgatgcg gagcctgccg ttcaaagtca
     9901 tctgcctgtt gggtttgaac gacggagatt ttccccgcaa taccaaagcc gccgtattcg
     9961 acctgattgc caaacatccc gccaaaggcg accgcgcccg ccgcgatgac gaccgctacc
    10021 tcttcctcga agccctcatc agcgcgcgcg aaatcctcta cctgtcctac atcgggcgcg
    10081 acatccgcaa agacgaagag cttgctccgt cttcgctgtt gggcgaactc atcgacacag
    10141 ttgccgctat ggcgggcatc ggtagccgcc aacttgcaca aaactggata gaacagcatc
    10201 cgctgcaagc cttctcgcgc cgatattttc aagaaggcgg acgttcagac ggcatattcg
    10261 gcacgcgtac cgactacgcc actgcgctcg gacaaacgcc cgaaccgccg caaccctttt
    10321 tcgaccaacc cgtagaaaac gccgaacctg ttgccgaaat cggacaggac gaatttatcc
    10381 gtttctggcg caaccccgtc aaagtatggc ttcagcagca gcttgcgtgg agcgaacccc
    10441 atatcggcga agcctgggag cctgccgaac ccttcgagcc gcaacacgcc gatcaaatcg
    10501 ccgaaaccta catcgaagca cggcgcgaag gacgggattt tgcccaaacc gccgcccgca
    10561 tcggggcgga aagcctcctg ccgtcgggag agttgggcag actttggcag cagaacttcc
    10621 aaactgctgc caaacaaatc gacacggcgg ttttaaacag ccccaaactg ccaccgcttt
    10681 catatgccat accgtcggac gggcaaatcc tgaaaggcag cttgggcaat ctgtaccgct
    10741 gcggacaagt gttttacgcc tacggcaaac ccaacgcgcc gcaacgtatc gcttttctgc
    10801 tggaacacct gatattttgc gccgttatgc cgtctgaagc cgaaacgcgg caaaccttta
    10861 tcgtccaatc cggagaaacc gaaatattgg cggaaatcgc gcaagacagg gcattgcagc
    10921 tattgtcgga atggatggcg tttttcaata tcgggcaaaa ccgcccgctg ccgttttttg
    10981 ccaagacctc gcttgccgct gccgaagcgt ttgcccaaaa acaagattgg gaagccgccc
    11041 tgaaaaaagc ccaaaccgcc tattacggca acaaagtcag caaagggcag aaagactata
    11101 ccgaagtcgc cctcgtgttc ggcaacgcaa gccaaaaccc actcgaacaa cccctgtttg
    11161 aaaacctcgc ccgcctgctt gccgacacgc ttgccgcagc ggaaaaaagg gaaggggccg
    11221 gagcagcctg acgggatgga aaggcaaatg ccgtctgaaa acagcaaaaa ggttttcaga
    11281 cggcattttg tttatcgtgt ttaaggtgcg ggatagaatg tgggcgcgcc gggaccgacg
    11341 ggcaggccca aaacaaatac ccaaatgcag aataaggcaa tccacgcaat caagaagaaa
    11401 gcggaatacg gcaacatcat agaaatcagc gtacccacgc ccgcatcttt tttgtatttg
    11461 atcaccgtcg ccataatcag cccgaaataa ctcatcatcg gcgtaataat attggtaacg
    11521 gaatcaccga tgcggtaagc ggcttgaatg acttcgggcg cgtagccggc caacatcagc
    11581 atagggacga aaatcggcgc agttaccgcc cattgcgcgg aggcggagcc tatcatcaga
    11641 ttgataaaag cacaaattaa aataaaaccg ataaacaaca cgctgccgcc caagccgact
    11701 tcttttaaga acgtcgcccc tttaacggca atatattgcc caatattcgt ccaattaaaa
    11761 aatgcgacaa actgtgcggc aaaaaagatg atgaccaaat aaagccccag agtactcatc
    11821 gattcggcca tcgcattaac gacttcctgt tcgccgcgca aacttcgggt tacccggcca
    11881 taaacaatgc ccggcagtgc aaacaacaag aaaataaaaa caacaattga ttttaaaaac
    11941 ggcgaaccgg aaaccaatcc tgtttcagga tgacgcaaaa taccgtcggc agggacgatg
    12001 ctccaagcca atagggcgga taaggcaaca aacaccacgc cagcccaaat taatccttta
    12061 tattccaaag gcgtgatttc attggaatgt cgaatgtctt tttcttcttg tgacaaatct
    12121 gattgataag ggcccaattg cggttcgacg attttttcag taacaaaata accaatcaaa
    12181 gcaatcacaa acgtactggc taccataaaa aaccagttgg cttcagggcc tacgacgtag
    12241 tcgggatgga tgatttgcgc cgcctgttgg gtgatgcctg ccaagagcgg atcgattgtg
    12301 cctaagaaca gattggccga ataaccgccc gaaacgccgg cgaaagccgc agccagaccg
    12361 gcaagcggat ggcggccgag ggaatgaaag atgatggcgg acaaagggat taggacgaca
    12421 tagcccaatt cagaagcggt attagataaa atccctgtaa aaacaaccat aaaagtagtg
    12481 agtttgcgtg gagattttgt gagcaataag cgcattaatg cggaaatcaa gcccgatttt
    12541 tccgcaatcc ccacgcccaa taaagaaacc aacaccgttc ccaacggcgc gaaaccggtg
    12601 aaatttttaa cggtatgcgt caggattttg atcaaaccgt cggcattgag caggctgaca
    12661 acgtaaatca aaccgtcatc ggcacgtcct ttcgcaccaa cagggcgcgg atcggggacg
    12721 gatagtccga aatatgcacc gacggcagag gcaatcagca ataacacaat gaaaataata
    12781 aaaagcgtaa ccggatgcgg caacatattg cccagccatt cgactgtgcg taaaaatcgc
    12841 ccgccccgcc gcgtatcggt ttgattcatc tgtttctcct taaaaaaata tatcgtgtgc
    12901 attaaatttt tgaatgtaac atgaagtgtt ttgtaagcaa actaaaataa tcgcatatgt
    12961 atataaccgc aatagattaa aacagaaaat gccctcatcg ttattatcgt aaaggcggga
    13021 atccgaaagc tcgacgttaa agccttatca ggaatgactg gaactcaaaa aacggtttcc
    13081 cgctttcgcg gaaatggcgg gattttcggt ttttatttgc aaaaatgccg gagaagaatg
    13141 cagtttacat tttgaatggt attgttatta attgtaaata aatattggaa ggtaaatgat
    13201 atgaaaaatg aaattcaaaa aattatggac aaatatgatc cctggcatga agatgatttt
    13261 gagtcgtatg aaaacattgc cagagatgta tcgctgatga cagataaaac gttcattgaa
    13321 cattatttgt tagaagttta ttcagaagaa aacggacatt ttaaccaaga gaatgtccat
    13381 gcaatgatag aagaaattaa aaatgcaatt tagtgaatat gtatttttag aagaaggctt
    13441 tgtgtcattt tgatgccgtc tgaaagggtt tgttcgtttc agacggcatt gttaccaagg
    13501 cttgattatt tccggcgcag gtcgggatgg ttttccaagt tgtccatcat tatcccgatg
    13561 atgcgcgggg cggttttgtc caaatcgaaa cgttcgcagg aagagaacca ccgccaaatc
    13621 agcccgtcca aggttgattt gatgaagata actgccgttt ccttgtccaa atcgtcagcc
    13681 aaatcctgat tttccaccgc ttcggtcaaa acggcggtaa ttttctcgcg ccagattgcc
    13741 tgatgcttgc gggcaatggc gataacggcg gcgttttgct ccgtgtgttc gcattttaaa
    13801 aacaggatgt tgtggaattt gtagtagatg tcgttgcttt gcagccgctc gaaaaagtgc
    13861 agcagcgtgt ggcggaatac cgcccaagac cctccttcgg catcttgcgc gatgcagttt
    13921 tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca agtcttcctt atttttgaaa
    13981 tgccaataga gcgcgccgcg cgttacgccg gcggcttggg cgatttcgtt gagcgaggtg
    14041 cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa gcatcaggtg ttctttggtt
    14101 ttcaaggctt cggttttggt ttttctcata atggcgtttt cgtttcgggt cggtttgatg
    14161 agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt gtatgtataa tgaaacccat
    14221 gaaattgaga ctacatctca actttgaaaa caggcataac ctgctttgca acccgttcga
    14281 acattcgggt atcaaagcag gcggttttta tatcct
//
LOCUS       DAWFJW010000050        13629 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFJW010000050 DAWFJW010000000
VERSION     DAWFJW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.050"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(120..1034)
                     /locus_tag="WGR19_001170"
     CDS             complement(120..1034)
                     /locus_tag="WGR19_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0798706.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGR19_001171"
     CDS             1290..2759
                     /locus_tag="WGR19_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0798707.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGR19_001172"
     CDS             3011..3382
                     /locus_tag="WGR19_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798708.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMIGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGR19_001173"
     CDS             3455..4864
                     /locus_tag="WGR19_001173"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0798709.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWSGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDVAIPRPAALESLETLLQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4911..5705
                     /locus_tag="WGR19_001174"
     CDS             4911..5705
                     /locus_tag="WGR19_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798710.1"
                     /translation="MTVPMQETRFSILLDELAAKQEAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            5893..6669
                     /locus_tag="WGR19_001175"
     CDS             5893..6669
                     /locus_tag="WGR19_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0798711.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7336..9792)
                     /locus_tag="WGR19_001176"
     CDS             complement(7336..9792)
                     /locus_tag="WGR19_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0798712.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNTLRLHKREEMPSEQ"
     gene            complement(9789..10967)
                     /gene="hflX"
                     /locus_tag="WGR19_001177"
     CDS             complement(9789..10967)
                     /gene="hflX"
                     /locus_tag="WGR19_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0798713.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10972..12252)
                     /locus_tag="WGR19_001178"
     CDS             complement(10972..12252)
                     /locus_tag="WGR19_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0798714.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVGGMAGSSITLAPEDSPERGVTLYLQD"
     gene            complement(12405..13124)
                     /locus_tag="WGR19_001179"
     CDS             complement(12405..13124)
                     /locus_tag="WGR19_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0798715.1"
                     /translation="MDTTRIVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAMLAS
                     GIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGM
                     EFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAAAP
                     YDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKL
                     GVLYYGDESTQYEPAPIRVDK"
     gene            complement(13195..>13629)
                     /gene="radC"
                     /locus_tag="WGR19_001180"
     CDS             complement(13195..>13629)
                     /gene="radC"
                     /locus_tag="WGR19_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0798716.1"
                     /translation="FAVVREIGRRILAEELQESIVLSDPDTVADYLRFHLGQEKVEVS
                     VALLLNRQNQLIAVRELSRGTVAENTIYIREIVKLALDEYADSLIIAHNHPGGSPEPS
                     QEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSFRQLGLMP"
BASE COUNT         2971 a         3537 c         3824 g         3297 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtgttga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacctgaacg gatgatcggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg ctggcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaagcc ttggacaaat ggagcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcgaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaagggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacggaaata tccacaatat
     4021 cgccgtgaag gggatgtttg acgattgtca ggacattgtg aaggcagtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaactcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acaaatgggc ctgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caatttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtcgcggcag gcaaaggctt
     4501 tgacctgcga tttgccttgg acaaagtcgg tggcaaatac ggctttactt ccggcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaac
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgtc gccattccgc gccccgccgc gctggaaagt ttggaaacat tgctgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtatgggcgg cattccgtcc gaacttaaga ttgaaagaaa atgaccgtac
     4921 ctatgcagga aacccgtttt tccatactgc tggacgaatt ggcggcaaaa caggaggcag
     4981 ccatcgcccc ccacctgctt gccgacggga gcaaggtatg gatacgcaaa gcaggcaggc
     5041 acaatgcacg atggcgttac gcgctgctcg gtatggttgc ccgatatttg aaactggggg
     5101 tgttgaagcc ggtccccagc ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc
     5161 tgtacgaatt gcgcgcggcg gggatagccg ttcccgaatt gctcgccgtc aggaaaaatg
     5221 cccttatgtt cggcaatttg gaaggcatcc cgctcgatac gcaaatccgc caagaagccg
     5281 aagccggaaa ggcggacgct tggcttgccg gtttggaagc cattgcacgc gtgcataaaa
     5341 aacagcagtt cctcagtcag gcgtttgcgc ggaatatgat gtcggacgga aaaaacatca
     5401 gctttctaga ttttgaagac gacccttccg aagtcctgac catagcgcaa tgccaagccc
     5461 gcgattggct ctgctacatc cattcgaccg cgctgatatt gaaaaacggc ggactgctgg
     5521 aagcggctgc ggaaaaatgg ggcggcgtat tgtcggatca gcctgccgaa atacaaaggc
     5581 tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac ccccgctggg
     5641 gacgggacgc gctcaggctg gcagcctcga tttccctgat ttcccttgcc gatataccgc
     5701 cataagccgc cgcccgcccc gcgatgccgt ctgaagccct tcagacggca tataaccaac
     5761 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttggcttt atcccgaatc
     5821 gggcagacat ttccactctg ccgcacgtcc cgaacaagca ctatccattt cttttactga
     5881 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
     5941 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
     6001 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgtgcata cagcgtcgcc
     6061 tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga cggcccgctg
     6121 accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc
     6181 ggaactctgg ttgccggcga cctgaatccg ggcaaacacc tttacttgtt gagcaccggt
     6241 accggcatcg cccctttctt gagcatcacc aaagaccccg agatttacga gcaatttgaa
     6301 aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tagcgtacta cgaccgcttt
     6361 accaaagaat tgcccgaaca cgaatacctc ggcgacttgg ttaaagaaaa actgatttac
     6421 tacccgattg tttcccgaga agaattcgaa caccacggcc gcctaaccga cctgatggtg
     6481 agcggcaaac tgtttgaaga catcggcctg cccaaaatca acccgcaaga cgaccgcgcg
     6541 atgctgtgcg gcagccccgc gatgctgaaa gacacctgca aagttttgga cgatttcgga
     6601 ctaaccgttt ctccgaaaac aggcgtgcgc ggcgattacc tgattgagcg cgcgtttgtg
     6661 gatcaataag ctgcttgaaa atgccgtctg aacggcatca gcgataaaaa aacccgaatc
     6721 ctgccaatca ggattcgggg tttttatagt gggtaaaaat tagaaatgca catttccgtc
     6781 attccaacac aggtgggaat ccggacttac ctgtttcatt atcgtttgaa aataaaagag
     6841 aaaccaaaca tatagattcc cgcttgcgcg ggaatgacga cggtgttttg ccgtagccaa
     6901 agcagaaaaa catccctatt gtcatttcat catgatatga caataggatg ttaaaaatta
     6961 attatcatta tatgattgat atataagaaa aactgtccca ataagaaatg ttatgcagaa
     7021 aataaaatga aaaggatggt tttcattgaa aatggttgtt ctgccaccgt catacacaga
     7081 gccagtattt ggaaaaaaat gatccatatt gacatcatta ttccatttgt cgcataaatc
     7141 cactttttaa ttttttccac attaatataa tctcttattt aataaaatca aaataaaaat
     7201 caaatgccta acaattcgat ctcaaatttt gaacgccaac gtgtttgagc ggtaacaata
     7261 catactttaa gtttgcaatc cttcatcgtc ttatttttgt tttcatcaac tatacatctg
     7321 aagcagcgcc gtccgtcatt gttcggacgg catttcctcc cgtttgtgaa ggcgcagggt
     7381 gttgcccaaa accgccagcg aactgaagct catacccagt gcggctatcc acggttggac
     7441 atagcccaaa acggcaagcg gtacggcaat gatattgtac gcgcccgccc atatcaggtt
     7501 ttgccggata atatggcggg tgcgccgcgc ctgatcgagc aggtgggcga cggtacgcaa
     7561 atcttcgttc aataacacaa tgtccgcgcc gtccctcgca atatccgtcc cgcccgctgc
     7621 ggcggcggat acgtctgcct gcgccaaaac gggcgcgtcg ttgatgccgt cgcctatcat
     7681 cagcactttt ttcccttctt tttgcaaggc tttgacgtat tccagcttgt cctcgggcat
     7741 ggcttgggcg cggtagtgcg tgatacccag ggcgcgggcg gtttcggcaa cggcggtttc
     7801 gcggtcgccg ctgaggatgt gcagggtcag gtttttgcct gccaactgcc gcaccgcctc
     7861 cgccgcgctg tctttcaagg ggtcggtcag gtagaacacg gcttggaaac cgctttgact
     7921 gccgaggtaa accgcgctgc cgccgccttc tgtttgcggt tctttacctg aaatttcggc
     7981 gacataggat gccctgccca atgcccaaac ctgtgtttcg ccgttgacgg tcagttgcgc
     8041 gcccacgcct tcgccgatgc ggttgaggcg ttgtttaata gcaatgtcgg ggacgctgcc
     8101 gtctgaaatg cggcagttga ggatggcgcg ggcaagggga tgttcggact gttgttctaa
     8161 agcctgcgcc accgcgagaa caaaggcttc gtctgtgcct ctcaacaatg agatacggcg
     8221 gacggcgggt ttgccttggg tcagcgtgcc ggttttgtcg aagatgatgt cggtggtttg
     8281 ggcgagggtt tcgattgcct gctttccgcc gattaaaata ccttcgcgcg ccagcgtacc
     8341 ggtagaagct gccagcgcgg tcggcgtggc aagcgataag gcgcaggggc aggtaatgac
     8401 cagcagggcg acggtaatcc acaatgcggt gtgcgcgtcg gcgtacagcg tccagccgat
     8461 gaagacgggg acggcaagca ggagttcgcc gaatatgaaa gacgaggcgt attgttccgc
     8521 caactcggca gtgcgcggtt tttgcgctaa ggcgcggtcg agcaggcgga cgatgtgcga
     8581 cagtcgcgtg ccgccgccgg tgcggtcggt gcgtataatc agggggcttt gcgtgttgag
     8641 tgtgccggcg gttacttttt cagacggcat tttggcgacg ggcaggctct cgccggtcag
     8701 catagattcg ttgacggcac tgcttccttc cagcaccgtg ccgtcaacgg ggatggtttc
     8761 gcccggtttg accagcacga tatcgcccgc cttcaatttg acgacagctg cctcgcaggt
     8821 ttcctgcgta tcggggtaat cgggcatatg atggcaaaac gcaggaatca gcttcaccag
     8881 cctctcggcg gcatcgcctg ccttacggcg ggcaatgtgt tccataaagc gtccgcccag
     8941 caggaaaaac agcagcatcg cgatggattc gaaatacatc ccctgccccg catttgtcgc
     9001 aaggctgtaa acgccggcga taaaggtcat gatgatggcg acggtaatcg gcgtatccat
     9061 gccgacgcgg cggtttttca agtcgcgcag cgcgccttga taaaacggga cggcgcaata
     9121 gaatacgacg ggcagcacca ttaaaaagcc gccccaatgg aggatttgca ggaaatcggg
     9181 ttcgatgtcg ccgccgtaaa ggtaggtcgg cagcgcgaac atcatcgtct gcatcatccc
     9241 cagcccggca acggcgaggc ggacgatgta ttgtttgcgt tctttttggt tggcggcttc
     9301 gattttttgc gcgtcatagg gtgcggcggt gtagcctatc tgcctgattt tcaacagaat
     9361 gtcggaaagg cggattttgc cgtcgtccca gacgacgcgg cagcggtgcg tgctgtaatt
     9421 gagatcgatg cggacgatgc cgtctgtacg caaaagctgc tgttcgatca gccagacgca
     9481 ggcggcacag gtgatgccgc cgagcattaa aaccgcctcg cgcgtgccgc cgtgggtttc
     9541 cacaaagtcg gactggactt cgggcaggtc gtacaggcgg atttggtcga ggatttcttg
     9601 gggcggcagc tcggtttttt gcgcgtcggc ggtgcgttgt ttgtaataac tgcccaagcc
     9661 cgcgtcaata atgctttgtg cgaccgcctg acagccggcg cagcaggttt ctcggtcttc
     9721 gccttcgtag cggacggtca gatgcaggtt ttcgggaaca tccagcccgc agtggaaaca
     9781 ggtttttttc atggcatttc ggtttcgtct gtgtttggtg cggcggcaca agactcggca
     9841 atggcttcgc gcagtgcgtc tataccggta ttttcagcaa cggaaatgcg gacggcggca
     9901 atttttcccg cagcgtcgcg ccatatgccc gtgttttgtt cttcagacgg cagcaggtcg
     9961 gttttgttgt acaccttgat gcacggaata tcgccggcat ggatttctcg cagtacgttt
    10021 tccacgtctt caatctgctg tccgctgttc ggagcggcgg catcgacgac gtgcagcagc
    10081 acatcggctt gcgcggtttc ttccagcgtg gcggaaaagg cggaaatcag tttgtgcggc
    10141 agatcgctga cgaatccgac ggtatcggtc aggatgatgc tgcattcggg actgatgtac
    10201 agccgccgcg ccgtggtgtc gagtgtggcg aaaagctggt ctttcgcata tatgcccgac
    10261 ttggtcagcc ggttgaacag gctggatttg ccgacattgg tatagccgac cagcgcaaac
    10321 gttttgattg tgcccgattc gcgggacttg cggcgcaggg cgcgctgttt tttgaggttg
    10381 gcaagctgtt ttttcaaggc attgatccga tgggcgatca atcggcggtc ggtttccagt
    10441 ttggtttcgc cggggccttt catgccgata ccgccgcgct ggctctgcag atggccgtaa
    10501 ccgcgtatca agcgtcccgc caaatggctc aattgcgcca actcgacttg cagcctgcct
    10561 tcctgcgtgc gggcgcggcg agcgaaaatc gccagaatca gccctaccct gtccaatacg
    10621 cggcatttca gttctttttc aaggttgcgt tcctgcgtgg gcgtgagttc gtggttgaat
    10681 acgaccaaat cgatgccgtc tgcggcaact gcttctgaca gctccgccgc cttgcccgtg
    10741 ccgacaaaca gcgcggtgtg cggacggtcg cgtttggcag tctccacgcg cacggaatcg
    10801 ccgcccgccg ctttgaccag ctcgacagct tccgccaatg ccgtctgaaa accgttcaga
    10861 cgggcggcac tactgcccgt accatctttg tccaacatta cgcccaccag catgacgcgt
    10921 tcgggttgcg cttgggtact gccgttgccg cctgttctgc ctgtcatgta ttcaatcctg
    10981 taaataaagg gttacgccgc gttccgggct gtcttccggt gcaagcgtga tgctgctgcc
    11041 cgccattccg ccaacgctga agacgatgcc gcccgcctcg tgccttccta tggacaggcg
    11101 gctgccgggt cgctgccggt aggtataacg ggtacacgct tccaaacacc agcgtccgcg
    11161 ccagtggatg aaatattttt cgccttttct aaccgcaagt gtctcgccgc cgtttacctc
    11221 aaaataaggg taatcgggtt ttcgggttct aaactgccat ttcgcctgcg cgcgccgtcc
    11281 gttgcggaca tagtcgaata ccgccgtata aagcgtaccg tattccaaag gcttgagcgg
    11341 gaaaagcgcg aattggtacg cggtcagcct gccgttgggg tcgttgccgg cggttaaaac
    11401 cctgacgggg cggatttcgt ttttaccctg atacagcttg aaacttttca tcgtaatttt
    11461 gcctgccgcc tcggaaaaat caatgctggc aggattgccc gtgatttcat attccggcac
    11521 ggggtctgga cgctcgccgt ggaaataagg cagtgcgccg ttgccgacgg gataggctgt
    11581 atagagcagc tcctgtgcgg gcatggcttc gtcggtgtac acgaccgcac cgttatggca
    11641 ggcgttgcgg taatatttcc gtcctgcttc cggctgattt ctgccttggg cgcaatgccg
    11701 ctcaaacctg ccgttgccct gattgaatac gagaacggtt ttaccgtttt cgcgcacaaa
    11761 tgccgctcct gcctcatccg tatggcggtc aagtagggaa aggcggtggt agattgcgct
    11821 catcaatccg tccacttggc gttgctgcgt gcggatgtcg ctgtcggacg attcggcggc
    11881 ttcctcttcc gtgctgatgt tttcatgcac gccgttgtag agatacccgg caaggcgtgt
    11941 gcgttcggtc agcttttgtg cggtgtagtg cggattgtcg ggatggtgtt cgccgtgtcc
    12001 gtcttcggga ttgagcgtga gatagcgtgc gtgcctgcgg gcggaatttt ccaaaaccgg
    12061 cgcgtgtgcc agcttgtgca aaccgatttg ggcgcggatg cggttgaggt agtgcagcgc
    12121 atcgccgccg tccctgattt gcggtgcgga cggatagaca agttcgcccg cgggcaggga
    12181 ttggttttgg gtatgataga aaacgcctgc cgccgaaccc aacaggagca gccgaataaa
    12241 aagggatttc ataatgccgt cctcccgacg cggcaacggg gtgttttatc aaaacggcaa
    12301 acttgccaat gcggtttccg attgcagatt gccaaaccgg caaacctttc cccgtgccga
    12361 tgccgaacct gatgccgtct gaaccttcag acggcatcgg atgtttattt gtccacccgg
    12421 atgggtgcag gttcgtattg tgtcgattcg tcgccgtaat acagcacgcc gagtttgatg
    12481 gggattctgc cctgtgattt gcggtgggca ttggaatccc tcagggaata ggcacaaccg
    12541 caatattcct gctggtagaa gttttcacgt ttgctgattt caatcatgcg cgcgctgccg
    12601 ccgcctttgc gccagttgaa atcccaatac accacatcat cgtaaggcgc ggcggcgcgg
    12661 tgtccgcagt cgttgatttg cgccatattt ttccagcgtg aaatgcccag cgaactggta
    12721 aagacgggga acccgtgttc atgcgcgtat tgcgcggctt tttcaaaacg catatcgaaa
    12781 cacatggtgc agcggatgcc gcgttccggc tcaaactcca tgcctttggc tttggcaaac
    12841 cattctttgc ggtcgttttc gtagtcgtcg tctttatcga tgaaaggaat gccgaacttt
    12901 tccgcaaagc gcacgttttc ctcttttcgg agcatatact ctttgtgcgg atggatattg
    12961 ggattgtaaa aataaatggt gtagtcgatg ccggaggcaa gcatcgcctc catcacttcg
    13021 ccgctgcacg gggcgcaaca ggaatgcagc aagacttttt tatgtccgcc gggcgggacg
    13081 agtatggggc ggtcgatgtc ggtaacgatg cgtgttgtgt ccatagtttc aaatagatgt
    13141 tctgtttggt gcaaacggct attttatcag agccgccgca tgtaaaacag ggggtcaggg
    13201 catcagcccg agctgcctga acgaacggac ggtttgcgcc gttacgataa aatggtcgag
    13261 cagcgacaca tcgaccagcg acattgcctg tgccagccgc cttgtgaaca tgatgtcttc
    13321 ctgcgaaggt tcgggcgagc cgcccggatg gttgtgcgca ataatcaggc tgtcggcata
    13381 ttcgtccaat gccagtttga cgatttcgcg gatgtaaatc gtgttttccg caaccgtacc
    13441 gcgcgacagc tctctgaccg caatcagttg gttttggcgg ttcagcagca gcgcgacgct
    13501 gacttcgact ttttcctgcc ccaaatgaaa gcgtaaataa tcggccaccg tatccggatc
    13561 ggacaggacg atgctctcct gcaattcttc cgccaatatc cgccgcccga tttccctgac
    13621 cacggcaaa
//
LOCUS       DAWFJW010000051        13599 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFJW010000051 DAWFJW010000000
VERSION     DAWFJW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.051"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..79)
                     /locus_tag="WGR19_001181"
     CDS             complement(<1..79)
                     /locus_tag="WGR19_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0798717.1"
                     /translation="MKPVVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WGR19_001182"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WGR19_001182"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0798718.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKEISGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIAGLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERLRFTTSHPREFTDAIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WGR19_001183"
     CDS             complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="WGR19_001183"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0798719.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKGII
                     RREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVERRV
                     RAWLSDRDAAN"
     gene            complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WGR19_001184"
     CDS             complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="WGR19_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0798720.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQDLLRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4798..5862
                     /locus_tag="WGR19_001185"
     CDS             4798..5862
                     /locus_tag="WGR19_001185"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0798721.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVGAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5882..6847
                     /locus_tag="WGR19_001186"
     CDS             5882..6847
                     /locus_tag="WGR19_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0798722.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGGGVAADIGAGLADALTAPLDHKDKSLQSLT
                     LDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLES
                     GEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPDGKAEY
                     HGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELAAAELKADEKSHAVILG
                     DTRYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQ"
     gene            6986..7663
                     /gene="tsaB"
                     /locus_tag="WGR19_001187"
     CDS             6986..7663
                     /gene="tsaB"
                     /locus_tag="WGR19_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0798723.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETRLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSGYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            7660..8100
                     /gene="rimI"
                     /locus_tag="WGR19_001188"
     CDS             7660..8100
                     /gene="rimI"
                     /locus_tag="WGR19_001188"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0798724.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSLSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFTHLPEDTQRLLLE
                     VRAGNTAAQALYTAHGFSITGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8094..8828
                     /locus_tag="WGR19_001189"
     CDS             8094..8828
                     /locus_tag="WGR19_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0798725.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPAPSAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            8890..9531
                     /gene="pyrE"
                     /locus_tag="WGR19_001190"
     CDS             8890..9531
                     /gene="pyrE"
                     /locus_tag="WGR19_001190"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0798726.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIQFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9605..10039
                     /locus_tag="WGR19_001191"
     CDS             9605..10039
                     /locus_tag="WGR19_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798727.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10036..11346
                     /gene="argA"
                     /locus_tag="WGR19_001192"
     CDS             10036..11346
                     /gene="argA"
                     /locus_tag="WGR19_001192"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0798728.1"
                     /translation="MSAPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVLLISGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTLHLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11508..13523
                     /locus_tag="WGR19_001193"
     CDS             11508..13523
                     /locus_tag="WGR19_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0798729.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAKANAETRTRFLENDKARALS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIAEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFVSDGINVQQFWTASSDGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLCETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
BASE COUNT         3053 a         4199 c         3627 g         2720 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccatacaac cggtttcata cacatctccc attaaagcca aacattatac agccgtccaa
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgtgaa aaagcgcaag agaaagtctt ctccgacttg ggtcgcgtac
      481 gcccgctcaa agaaaaaaat cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaat cagcgggctg tcgcaagtcg
      661 atatttcctt tcccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtagaaggtg
      721 gcgcggcatt cgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cactcaacga cgtattgacc gaaattgccg
      841 gcttggcgca gcaaggcgtt aaagaaatca acctgttagg tcaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg actttgccac tttgttgcgc atcgtccacg
      961 aaatccccgg catcgaacgc ctgcgcttta ccaccagcca tccgcgcgaa tttaccgacg
     1021 cgattatcga gtgctaccgc gacctgccaa agctggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctataccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgcgccatcc gccctgattt atgcctgagc agcgacttta
     1201 tcgtcggctt cccgggcgaa accgagcgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtcttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggccagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcaaca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac gtcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc aggtttgttt
     2641 tcagacggca tttgcgccgc gctttctttg ggcagcttgg cgacggcgtg gtatttgacg
     2701 gggtcgtttt cggcaagttt gtagccgttg ccctttttgg tgatgacgtg cagcagttga
     2761 gggcctttgc ggctgcgcaa gtctttcaat acgtccacca gattttcgac gttgtgtccg
     2821 tccacggggc cggtgtagcg gaagccgaag ttttcaaaca aagacagcga ctgtttggcg
     2881 tgttcggctt cttcggcaag ggttttgatt ttgtgttcga ctttttgggc aaactccatc
     2941 gcgccgggta ttttgtctaa taccttgccc gtttgcgctt tgacggtact caacaggccg
     3001 tgcatatcgc gcacgacgtt gctggcaagg tatttcggca gcgcgccgac gttgggggaa
     3061 atcgacattt cgttgtcgtt gaggacgacc agcaaatcca catccatatc gcctgcgcaa
     3121 ttcaaggctt caaacgcctg ccccgccgtc atcgcgccgt cgccgatgat ggcgacgctg
     3181 cggcggtcgc tgcccaagag tttgtctgcc gccgccatgc ccaacgccgc gccgatggaa
     3241 gtggacgaat gccccacgcc gaacgcgtcg tattccgact cgcaacgttt cggaaaaccc
     3301 gccaagccgc cgtattggcg catggtgtgc atctggtttt tcctgcctgt caggattttg
     3361 tgcggatagc tttggtgtcc gacatcccat accagcttgt cttcgggcgt atcgtacaca
     3421 tagtgcaggg cgatggtcag ttcgaccgcg cccaaattgc tggcgaaatg cccgccggtc
     3481 tgcccgacag attccagcag aaaggcgcgc aactcgccgg caaggcgcgg cagctgtttt
     3541 ttgtccagac ggcgcaaatc ttgcgggctg tcaatcaggt cgagtagggg gcttgggttc
     3601 atggtgtgtc ttttttatgt gtcgtccggg tgcaacgacc aattatatat caagagcgtg
     3661 cggctgacgg ctgattttgc cgtatgtcat tcgtcctgcc gcttggcgcg cgggtgggct
     3721 tcgtcataca ggcgggcgat gtggtcgaaa tcgagtttgg tgtaaatctg cgtggtcgaa
     3781 aggctgctgt gcccgagcag ctcctgcacc gcccgaatat cgcgcgaagc ctgcaacagg
     3841 tgtccggcgt agctgtggcg catcatatgc ggcgaaacgt gcctgccgtc gccgttttgc
     3901 gccgcccatt gcgccaaacg tttttggatt tggcgttggc tcaggcgcgt gccgttccta
     3961 ccggtaaaca aggctttgcc gtccgatgcc gtctgacgca gcggcagata gtttttcagg
     4021 gcttccacgc ttttgccggt cagcggcacc tgccgctgct tgcgcccttt gccggtaacg
     4081 tgtacccacg cctcgtccaa atagacatca tctgcattca agccgtgtat ctcgctcacg
     4141 cgcaaaccgc tgccgtacat cagttcgaac agcgcgtgat cgcgcaccgc cagcgggtcg
     4201 ccgccgtcca cgggcaaatc cagcatccgg ttcagccatt cctgcggcag tgctttgggt
     4261 acgcgctcgg gctgcttcgg cggtttgatg tcggcggtcg ggtcggtatg cagcagaccg
     4321 cgttccacca gccaaacaca atactgccgc caagacgaca gcttgcgcgc cagcgtccgc
     4381 gcattcaaac cgcgctgcga cagccgccgc aacgccgccg taaaatcgcg gcgcaacaag
     4441 tcctgcggca cgccgccttc agcttcagac ggcatttgtg ccagcagcgc aaacagttct
     4501 tccaaatcgc gccggtatgc cgccaccgtg tgctccgact tgccctcgcg cacgatgttt
     4561 tccaaataag cgtcaaaatg cgccgcaaac ccgtccaaaa ccataaccgc cccacacaaa
     4621 tatcaaaaaa acagtgaaaa cattatcata aacgacacac caaaaccccg aactgcccaa
     4681 aatccgaccc gtccgcccgc cgttatcgtt aacataaaat caatgagccg tttatggttt
     4741 tttgccgtaa aaaacattat aatccgcctt atttacctat tgcccaagga gacacaaatg
     4801 gcactcgtat ccatgcgcca actgcttgat catgctgccg aaaacagcta cggcctgccc
     4861 gcgttcaacg tcaacaacct cgaacaaatg cgcgccatta tggaagccgc cgaccaagtc
     4921 ggcgcgcccg tcatcgtaca ggcgagtgcc ggtgcgcgca aatacgcggg cgcgccgttt
     4981 ttgcgccacc tgattctggc ggcagtcgaa gaatttccgc acatccccgt cgtgatgcac
     5041 caagaccacg gcgcatcgcc cgacgtgtgc caacgctcca tccaactggg cttttcctcc
     5101 gtgatgatgg acggctcgct gatggaagac ggcaaaaccc cttcttctta tgaatacaac
     5161 gtcaacgcca cccgtaccgt ggttaatttc tcccacgctt gcggcgtatc cgtcgaaggc
     5221 gaaatcggcg tgttgggcaa cctcgaaact ggcgaagccg gcgaagaaga cggtgtaggc
     5281 gcagtgggca aactttccca cgaccaaatg ctcaccagcg tcgaagatgc cgtgcgtttc
     5341 gttaaagata ccggcgttga cgcattggcg attgccgtcg gcaccagcca cggcgcgtac
     5401 aaattcaccc gtccgcccac aggcgacgta ttgcgtatcg accgcatcaa agaaatccac
     5461 caagccctgc ccaatacaca catcgtgatg cacggctcca gctccgttcc gcaagaatgg
     5521 ctgaaagtca ttaacgaata cggcggcaat atcggcgaaa cctacggcgt gccggttgaa
     5581 gaaatcgtcg aaggcatcaa acacggcgtg cgtaaagtca acatcgatac cgacttgcgc
     5641 cttgcttcca ccggcgcggt acgccgctac cttgccgaaa acccgtccga cttcgacccg
     5701 cgcaaatacc tgagcaaaac cattgaagcg atgaagcaaa tctgcctcga ccgctacctc
     5761 gcgttcggtt gcgaaggtca ggcaggcaaa atcaaaccgg tttccttgga aaaaatggca
     5821 aaccgttatg ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ttgtaaacaa
     5881 aatgccgtct gaaccgttgt tcggacggca tttgattttt gcttctttga cctgcctcat
     5941 tgatgcagta tgcaaaaaaa gataccataa ccaaaatgtt tatatattat ctattctgcg
     6001 tatgactagg agcaaacctg tgaaccgaac tgccttctgc tgcctttctt tgaccgccgc
     6061 cctgattctg accgcctgca gcagcggagg cggaggcggc ggtgtcgccg ccgacatcgg
     6121 cgcggggctt gccgatgcac taaccgcacc gctcgaccat aaagacaaaa gtttgcagtc
     6181 tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct caatacgggc aaattgaaga acgacaaggt
     6301 cagccgcttc gactttatcc gtcaaatcga agtggacggg cagctcatta ccttggagag
     6361 cggagagttc caaatataca aacaggacca ctccgccgtc gttgccctac agattgaaaa
     6421 aatcaacaac cccgacaaaa tcgacagcct gataaaccaa cgctccttcc ttgtcagcgg
     6481 tttgggcgga gaacataccg ccttcaacca actgcctgac ggcaaagccg agtatcacgg
     6541 caaagcattc agctccgacg atgctggcgg aaaactgacc tataccatag atttcgccgc
     6601 caaacaggga cacggcaaaa tcgaacacct gaaaacaccc gagcaaaatg tcgagcttgc
     6661 cgccgccgaa ctcaaagcag atgaaaaatc acacgccgtc attttgggcg acacgcgcta
     6721 cggcagcgaa gaaaaaggca cttaccacct cgcccttttc ggcgaccgcg cccaagaaat
     6781 cgccggctcg gcaaccgtga agatagggga aaaggttcac gaaatcggca tcgccggcaa
     6841 acagtagcat ttgaaaaatg ccgtccgaac acgataattt accgttcgga cggcattttt
     6901 gcgccatacc gccctaggat acgacggcac ggcaggcaag ccggtttcac tatataatgc
     6961 cgtctgaacc aacgagaacc acaccatgca agccgatttc aaccgtcccg tcctggccat
     7021 cgataccgga acttcctatt tgtcgctcgc gctgcgcgcc gacggcgaaa cccgcctgtt
     7081 ccatcaagag gccggcagcc gccaatccga actgattctg ccggaaatcc gcactctatt
     7141 ccgaaatgcg ggcattaccg ccgccgattt gggggcgatt gtttatgcga aaggccccgg
     7201 cgcgtttacg ggactgcgta tcggcatcgg cgtggcgcaa ggtttggcaa cgccgtttga
     7261 tacgccttta atcggcatcc ccacgctcga tgccgccgcc tcgctgccgc cgccgcaaag
     7321 ctgcatactc gccgctgcgg acgcgcgtat gggcgaagtg ttttatgcgt ggttcgacac
     7381 gctgaaccgc cgccgtttga gcggttatca agtcgggcgg gcggcagaca tccggctgcc
     7441 ggagggatgt gccttttcag acggcatagg cagcgcgttc gcgttggaag aaacgccgcc
     7501 gttttcaggc aaacccgata tgccgaccgc cgccgacttc ctcaatctgg cggctgaagg
     7561 cggttttccc gccgtcgctg cggcacacgc cgagctgctc tacgtccgca acaaaatcgc
     7621 cctgactgcc aaagaacagg cggaacggag ggcgcgcctg tgaacatccg ccgcgccgtt
     7681 tatgccgatt gcgccgcact cgccgcgctc gatgccgtct gcaacccgtc cgcgtggacg
     7741 caacgccaat ttgagtctgc attggtttcg ctgtccgaac aggttttcct tgcggaaaaa
     7801 gacggcggga ttgccgcctt tatcgtttgg cagaacctgc ccgacgaatc cgaactgcac
     7861 ctgattgcca ccgcgcccga atgccgccgc cgtggcgttg cgtccgccct gctcgaatat
     7921 tggttcacac atctgcccga agacacgcaa cgcctgctgc tcgaagtccg tgcaggcaac
     7981 accgccgcac aggcactgta cacggcgcac ggcttcagca ttacgggcag gcggaaaaac
     8041 tattaccgta cagccgacgg taaaaccgaa gatgccgtct taatggagaa aatatgttaa
     8101 gcgcgcgcta cctccacctg cacgaagctt tgggtttggg tccgatgtgg ctgaaacagg
     8161 ccgccgccgt cctgccgccc aaaaacacac ccgcaccctc ggcacaggca cgtccccaaa
     8221 ccgtccgcgc cgccccgatc cgcccttccc aaccccataa cggtcaggcg cggctcgaaa
     8281 cgatgaaagc gttggaaacc gccgccgtac ctacgcgcaa acccgcgcct gaaaccgaaa
     8341 cgcctctgcc cggcctttca gacggcatcg cccccgttcc cgccgcttcg ggcatcacca
     8401 agcttgccgt cgtcagcctt tgcccaccga tcgaggatgc ggtttacggg cagctgttcc
     8461 acggcaaagc gggtgtcctg cttgacaaca tactcaaagc cgtaggactg gatgccgctt
     8521 atgtacacaa gaccagctgg gtgaaaaccg ccgccgtcgg caacccgatg ccgtctgaac
     8581 aggccgtcgc gaatgcgctg ggtcaaatcg ccagggaact cgacggctgc cgtgcccccg
     8641 ccgtcctttt cctcgggcag gcttttgtcc agcctgaacg gcaggcgatg attgaaactt
     8701 tgtgcggcag ccgtcccttc ttcatcatcg accatcccgc tcggctttta cgccaacccg
     8761 aacttaaagc ccgaaactgg caggtgttga aacagttgaa acacgccttg caggcaggcg
     8821 gcagttgaag caggccgcac ggggcggtag aatcacaact gcatcgcaat acctgacaga
     8881 aagcacaaaa tgaccgattt ccgccaagat ttccttaaat tctccctcgc ccaaaatgtc
     8941 ttaaaattcg gcgaatttac caccaaggca ggacggcagt cgccttattt cttcaatgct
     9001 ggcctcttta acgacggctt gtccacgctg caactggcaa aattttacgc acaatccatc
     9061 attgaaagcg gcatccaatt cgatatgctg ttcggtcccg cctacaaagg tattattttg
     9121 gcggcggcaa ccgcgatgat gctggcggaa aaaggcgtga acgtcccgtt tgcctacaac
     9181 cgcaaagaag ccaaagacca cggcgaaggc ggcgtgttgg tcggcgcgcc gcttaaaggg
     9241 cgcgtgctga ttatcgacga cgtgatttcc gccggcacat ccgtgcgcga atcgatcaaa
     9301 ttgattgaag cggagggtgc aacccccgcc ggtgtcgcca tcgcgctcga ccgcatggaa
     9361 aaaggcacgg gtgaattgag cgcggttcag gaagtggaaa aacaatacgg cctgcccgtc
     9421 gcccccatcg ccagcctgaa cgatttgttt attctgttgc aaaacaaccc cgaattcgga
     9481 cagttcctcg aacccgtccg agcctaccgt cggcagtacg gcgtagaata aaaacaaagc
     9541 atatgccgtc tgaaccgcct tacgcctcag acggcatcaa acctgacaca cacgaggaaa
     9601 taccatgccc gcctgtttct gcccccactg caaaacccgt ctctgggtca aagaaaccca
     9661 actcaatgtc gcccaaggct tcgtcgtctg ccaaaaatgc gaaggactgt ttaaagccaa
     9721 agaccatctg gcaagcacga aagaacccat attcaacgat ttgcccgagg ctgtttcgga
     9781 tgtcaaactc gttcaccgta tcggcacgcg cgccatcggc aagaaacaga tttcccgtga
     9841 cgaaatcgcc ggcatcctca acggcggtac aacccagccc gatattccgc ccgcaaccgc
     9901 cgccacccct gctgccgcac cgcaggttac cgtaccgccc gccgcgcccg cccgtcagga
     9961 tgggttcaac tggacgattg caaccctgtt tgcccttatc gtcctcatta tgcagctttc
    10021 ctacctcgtc atcctatgag cgcgcccgac ctctttgtcg cccacttccg cgaagccgcc
    10081 ccctacatcc gccaaatgcg cggcaaaacg ctggtcgccg gcatagacga ccgcctgctc
    10141 gaaggtgata ccttaaacaa gctcgccgcc gacatcgggc ttttgtcgca actgggcatc
    10201 aggctcgtcc tcatccacgg cgcgcgccac ttcctcgacc gccacgccgc cgcgcaaggc
    10261 cgcacgccgc attattgccg gggcttgcgc gttaccgacg aaacctcgct cgaacaggcg
    10321 cagcagtttg ccggcaccgt ccgcagccgt tttgaagccg cattgtgcgg cagcgtttcc
    10381 gggttcgcgc gcgcgccttc cgtcctgctc atatcgggca acttcctgac cgcccgtccg
    10441 ataggtgtga ttgacggaac cgatatggaa tacgcgggcg ttatccgcaa aaccgacacc
    10501 gccgccctcc gtttccaact cgacgcgggc aatatcgtct ggctgccgcc gctcggacat
    10561 tcctacggcg gcaagacctt acatctcgat atgcttcaaa ccgccgcctc cgccgctgtc
    10621 tcgcttcagg ccgaaaaact cgtttacctg accctttcag acggcattgc ccgccccgac
    10681 ggcacgctcg ccgaaaccct ctcggcgcag gaagcgcaat cgctggcgga acacgccggc
    10741 ggcgaaacgc gacggctgat ttcgtccgcc gttgccgcgc tcgaaggcgg cgtgcatcgc
    10801 gtccaaatcc tcaacggagc cgccgacggc agcctgctgc aagaactctt cacccgcaac
    10861 ggcatcggca cgtccattgc caaagaagcc ttcgtctcca tccggcaggc gcacagcggc
    10921 gacatcccgc acattgccgc cctcatccgc ccgctggaag aacagggcat cctgctgcac
    10981 cgcagccgcg aatacctcga aaaccacatt tccgaatttt ccatcctcga acacgacggc
    11041 aacctgtacg gttgcgccgc cctgaaaacc tttgccgaag ccgattgcgg cgaaatcgcc
    11101 tgccttgccg tctcgccgca ggcacaggac ggcggctacg gcgaacgcct gcttgcccac
    11161 attatcgata aggcgcgcgg cataggcata agcaggctgt tcgcactgtc cacaaatacc
    11221 ggcgaatggt ttgccgaacg cggctttcag acggcatcgg aagacgagtt gcccgaaacg
    11281 cggcgcaaag actaccgcag caacggacga aactcgcata ttctggtacg tcgcctgcac
    11341 cgctgaccgc aacggaaagc cgccgcagaa atgccgtctg aaccccgttt cagacggcat
    11401 ttccccgatt atatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttgtcctga
    11461 tttttgttaa tccactataa agacctgccc aaccctcaag gaccccgatg aaatcctacc
    11521 ccgaccccta ccgccatttt gaaaacctcg attccgccga aacgcaaaac ttcgctgcta
    11581 aagcgaatgc cgaaacgcgc acgcgttttt tagaaaacga caaggcgcgc gcgctgtcgg
    11641 acggcatttt ggcgcagatg caggacacgc ggcagattcc gttttgtcag gaacaccgcg
    11701 cgcggatgta ccatttccat caggacgcag aatatccgaa gggcgtgtac cgcgtgtgta
    11761 cggcggcgac ttaccgttcc ggctatcccg agtggaaaat cctgttttca gtggcggatt
    11821 ttgacgagtt gctcggcgac gatgtgtatt tgggcggcgt gtcgcacttg gtggaacagc
    11881 ccaaccgcgc gctgttgact ttgagcaaat caggcggcga tacggcgtac acgctggaag
    11941 tggatttgga agcaggggaa ttggtagaag gcggttttca ctttccggca ggcaaaaacc
    12001 atgtgtcgtg gcgcgatgaa aacagcgtgt gggtgtgtcc ggcttgggac gaacgccagt
    12061 tgaccgaatc gggctatccg cgtgaggtgt ggctggtgga gcgcggcaaa agtttcgagg
    12121 aaagcctgcc ggtgtaccaa attgccgaag acgggatgat ggtgaacgcg tggcgttacc
    12181 tcgatccgca aggttcgccg attgatttga ttgaagcgtc ggacggtttt tacactaaaa
    12241 cctatttgca ggtgtcggcc gaaggcgagg cgaaaccgtt gaacctgccc gccgattgcg
    12301 acgtggtcgg ctatctggcg gggcatcttt tgctgacgct gcgcaaagac tggaaccgcg
    12361 cgaaccaaag ctatccgagc ggcgcattgg tggcagtaaa attgaaccgc ggcgaactcg
    12421 gggcggcgca gcttttgttt gcgcctgatg aaacgcaggc attggaaagc gtggaaacga
    12481 ccaagcgttt tgtggtggcg agcctgctgg aaaacgtaca aggccgtctg aaagcgtggc
    12541 gttttactga tggcaaatgg caggaagctg aactaccgcg cctgccttcg ggtgcgttgg
    12601 aaatgaccga ccagccatgg ggcggcgatg tggtttacct tgccgccagc gatttcacca
    12661 cgccgctgac gctgtttgcg ctggatttga acgtgatgga actgaccgtc atgcgccgcc
    12721 agccgcagca gtttgtttca gacggtatca acgtgcaaca gttttggacg gcttcgtccg
    12781 acggcgaacg cattccctat ttccacgtcg gcaaaaacgc cgcgcccgac atgccgacgc
    12841 tggtctatgc ctacggcggt ttcggcattc ccgaattgcc gcattatctg ggcagcattg
    12901 gcaaatattg gctggaagag ggcaatgcct ttgtattggc gaacatccgc ggcggcggcg
    12961 agttcggccc gcgctggcat caggcggcgc agggaatcag caaacataaa agcgttgatg
    13021 atttattggc agtcgtgcgc gatttgtccg aacgcggtat cagttcgccc gaacacatcg
    13081 gcttgcaggg cggcagcaac ggcggactga ttactgccgc cgccttcgtg cgcgaaccgc
    13141 aaagcatcgg cgcgctggtg tgcgaagtgc cgctgaccga catgatccgt tatccgctgc
    13201 tctccgccgg ttcaagctgg acagacgaat acggcaatcc gcaaaaatac gaagtctgca
    13261 aacgccggtt gggcgaattg tcgccgtatc acaatctttc agacggcatc gattatccgc
    13321 ccgcgctcat taccaccagc ctgtccgacg atcgcgtcca tcccgcccac gcgctcaagt
    13381 tctacgccaa actgtgcgaa acctcgccgc aatcttggct ctactcgcct gacggcggcg
    13441 gccataccgg caacggcacg cagcgcgaag ccgccgacga actcgcctgc gtgttgctgt
    13501 ttttgaaaga gtttttgggc taagggcggg gagcggcact gccgccgcga atgaaaaaag
    13561 gtcgtctgaa actgcttttt cagacgacct tttttaatg
//
LOCUS       DAWFJW010000052        13434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFJW010000052 DAWFJW010000000
VERSION     DAWFJW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.052"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(290..1186)
                     /gene="argB"
                     /locus_tag="WGR19_001194"
     CDS             complement(290..1186)
                     /gene="argB"
                     /locus_tag="WGR19_001194"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0798730.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKMGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1356..2486
                     /locus_tag="WGR19_001195"
     CDS             1356..2486
                     /locus_tag="WGR19_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0798731.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2557..3408
                     /locus_tag="WGR19_001196"
     CDS             2557..3408
                     /locus_tag="WGR19_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0798732.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     LGMKKQH"
     gene            complement(3491..4153)
                     /locus_tag="WGR19_001197"
     CDS             complement(3491..4153)
                     /locus_tag="WGR19_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0798733.1"
                     /translation="MDSRKFTEASKQRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPELWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4248..6041)
                     /locus_tag="WGR19_001198"
     CDS             complement(4248..6041)
                     /locus_tag="WGR19_001198"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0798734.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6286..7395
                     /gene="proB"
                     /locus_tag="WGR19_001199"
     CDS             6286..7395
                     /gene="proB"
                     /locus_tag="WGR19_001199"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0798735.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIARIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7408..8670
                     /locus_tag="WGR19_001200"
     CDS             7408..8670
                     /locus_tag="WGR19_001200"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0798736.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDEKRIGAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(8895..11927)
                     /locus_tag="WGR19_001201"
     CDS             complement(8895..11927)
                     /locus_tag="WGR19_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ0798737.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSRAVCPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPGATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            12092..12772
                     /locus_tag="WGR19_001202"
     CDS             12092..12772
                     /locus_tag="WGR19_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0798738.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLDRYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRSIWR"
     gene            12801..13160
                     /locus_tag="WGR19_001203"
     CDS             12801..13160
                     /locus_tag="WGR19_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0798739.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALSNIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            complement(13292..>13434)
                     /locus_tag="WGR19_001204"
     CDS             complement(13292..>13434)
                     /locus_tag="WGR19_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798740.1"
                     /translation="NEFDEEDGWPDLSCSLHTFKTTLIAWNAFLQLPKSIHSVVETVI
                     EE"
BASE COUNT         2949 a         3823 c         3467 g         3195 t
ORIGIN      
        1 cccgataagt ttccgtgccg acaggtctag attcccgcct gcgcgggaat gacgaaattt
       61 caaagttatg gcgttatcgg aaaaacagaa aatcaaagcc ggagaattta tcccaaacaa
      121 ctggatttca aaaaaaccag attcccgcct tcgcgggaat gacggatttt aggtttctgt
      181 tttggtttct gttttcgcgg gaataacaac ttagaaattg cccgaaaccc caaaaacaga
      241 aaccggacaa acgggttttc cgccaaagcc ggcattttcc gactttgctt caggcatctt
      301 ccccaccgcc caaaatcatc gaaccgatac cggcatcggt aaagatttcc agcaaaagcg
      361 cgttgggcaa cctgccgtcg atgatatgcg tggctttcac accgttgacg gcggcttcga
      421 ccgcagaagc gattttcggc agcataccgc catacagcgt gccgtcggca atcagttcat
      481 caatccgttt cggcgtgagt ttggtcagca gattgcccat tttgtccatc acaccggcga
      541 tattcgtcat catcaagagt ttttcagcgt tcaattcttc cgccaatttg cctgctacca
      601 aatcggcgtt gatgttgaac gcttcgcctt tttcacctac gccgacgggg gcgacgacgg
      661 gaatgcagcc acgttctatc agccctttaa ccaaaccggt atcgatgctt tccaccgtac
      721 cgacctgtcc gatgtccacg ccattctgtt cgggcgtatc gatcaaaagt ttcttcgcct
      781 taatgaaatg gtcgtcgcgt ccgcttacgc cgaccgcgtg tccgccatat gtgttaatca
      841 tcgacacgat ttctttattg acatgcccgc ccaacaccat ttcgacaata tccatcgcct
      901 ctttgtcggt aacgcgcatt ccttggacaa actcaccctt tttgccgact ttttcaagca
      961 tcgcattgat ctgcggcccg ccgccgtgaa cgatgacggg atgaatgccg accagcttca
     1021 gcagcacgac atcgcgggca aacccttctt tcaaggcagg ttcggtcatg gcgttgccgc
     1081 cgtacttgat gacggcgacc gaaccggaaa accggcggat gtaaggcagc gcttcggcaa
     1141 ggatacgcgc tttgtcggcg gcggaaataa tgttttcaga ctccataacg gctccatatt
     1201 gttaacaatt tcggcttatg ttaacgccaa acgccgtctg aaacaatctt cagacggcat
     1261 ccggcagacc ttcgggcagg ttgaagcctt gcgatacggg cggtaaaatc gcggtttatc
     1321 cggttcgaga aaccggcgac agcaaggact ttcccatgat taaaatcagc acccgcttcg
     1381 atgccggctc ggtcgtcgtc aaagacctga ccgatccttc caacatccgc ctcgccctgc
     1441 gtcccgacaa cgcctccgat tttgcacaat ggttctactt ccggctgcaa ggcgcggcat
     1501 atcaaaactg catcatgcat tttgaaaacg cggcagaagc cgcctacccg aaaggctggg
     1561 aaggctatca ggcgtgtgcc tcatacgacc gccgcaactg gttccgcgta ccgacttcct
     1621 acgaaaacgg cgtactgacc gtcaatcata cgccgctgtc caacagcgtg tattacgcct
     1681 atttcgaacc ttactccgaa gaacagcatt taaacctcct cggcgacgcg caaggcagcg
     1741 gcctgtgccg catcgacgac ttgggcagca ccgtgcaagg gcgcgacatc aatctgctga
     1801 ccatcggcaa tcaggtcgaa agcgatctga aaatctggat taccgcccgc cagcatccgg
     1861 gcgaaaccat ggcggaatgg tttgtcgagg ggctgctcgg caggctgctc gatccgcaag
     1921 acccgacagc gcgcaccctg ctcgaccgcg ccacgttcta tatcgtcccc aatatgaacc
     1981 ccgacggttc ggcactgggc aaccttcgta ccaacgccgc cggtgcgaac ctcaaccgcg
     2041 aatgggaaaa tccgactttg gaaaaaagcc ccgaagtgtt cttcgtgcgt gaaagaatgc
     2101 tggaaaccgg cgtggatttg ttcttggaca tccacggcga cgaagggctg ccctttatct
     2161 ttgttgcagg aactgaaggc gtgccaaact acaatccgcg catttccgcg ttggaaacac
     2221 agttcaaaac cgcccttttg aacgccagcc ccgatttcca agacgaatac ggttacgaaa
     2281 aagatgcgcc cggtcaggca aacatgacct tggcaacaaa ctgggtcggc aaccgtttca
     2341 actgccttgc ctatacgttg gaaatgccgt tcaaagacaa tgccaacctg cccgacgacg
     2401 atttcggatg gaacggtcaa cggtcgttgc gcttgggcga agccgcgttg tccgccatcc
     2461 tcaacatcat cggcgatttg cgctgaaacc cttattgcag aaatgccgtc tgaagcatca
     2521 tgcctttcag acggcatttg aaaacgaaag cccgttatga ttacccatcc ccaattcgac
     2581 cccgtcctta tcagtatcgg tccgcttgcc gtccgctggt atgccctaag ctacatcctc
     2641 ggatttattc tttttacctt tctcggcaga aggcgcatcg cgcaaggctt gtccgttttt
     2701 accaaagaat cgcttgacga cttcctcact tggggcattt tgggcgtaat ttttggtggg
     2761 cgtttgggtt acgtcctgtt ttacaagttt tccgactacc tcgcccatcc gcttgatatt
     2821 ttcaaggtat gggaaggcgg aatgtcgttc cacggcggct ttttgggtgt agttattgcc
     2881 atatggttgt tcggccgcaa acacggcatc ggcttcctca aactgatgga cacggtcgca
     2941 ccgctcgttc cgctgggtct cgcttcggga cgtatcggca acttcatcaa cggcgaactt
     3001 tggggacgcg ttaccgacat caacgcattt tgggcaatgg gcttcccgca ggcgcgttac
     3061 gaagatgccg aagccgccgc gcacaatccg ctttgggcag aatggctgca acaatacggt
     3121 atgctgccgc gtcatccctc gcagctttat cagtttgcac ttgaaggcat ctgcctgttc
     3181 gccgtcgttt ggctgttctc taaaaaacag cggccgaccg gacaagtcgc ctcgctcttc
     3241 ctcggcggct acggcatatt ccgcttcatt gccgaattcg cacgccaacc cgacgactat
     3301 ctcgggctgc tgaccttggg gctgtcgatg gggcaatggt tgagcgtccc gatgattgtt
     3361 ttgggtatcg tcggctttgt ccggctcggc atgaaaaaac agcactgacc gcctgtgaaa
     3421 aataccgtct gaaacatgga aatccggttt cagacggcat ttttacgttt ggttcatgga
     3481 aacaaacgct tcaattctta ccgcaacacg ccttgtattt tctgccgctg ccgcacggac
     3541 agggatcgtt cctgcctgtt ttttcgcctt ccctgcggac ggtttgcggt ttgttgatga
     3601 ctgcctgcca gtagcggtag atatccgcca atgcataagg caactcggat tccagttccg
     3661 ccagctcgcc ttctgtgaat tgcagacgga tagtgccgtt gtcctcttcg tcgtaaatac
     3721 cgcccaatgc catgatggga taaaacaact cttcaaacgc ttcatcatcg acggcttcaa
     3781 accaatcggt cggcacaatg tccaagccgt acagataggc gttgcaccaa gtgtaaaaat
     3841 cgctgccgcc ctcgccatca tcgtagagcc acaattcggg cagcttttta tccgacatcg
     3901 cggcggttgt ttccatcgcc attgccaaaa ccagccgttc gatttcggaa cgttcggcgg
     3961 cggtaaattg cgattcgtcg cccaacactt cgggcagcca gtcgagcggt gtcaatttgt
     4021 ccggcccgct caacagcgcc gtcataaaac cttgaacctc gtcgcaacgc atcgtgttgc
     4081 cttgttcgct tttggcatcc aacaattcgc tcaaccgctg tttggatgct tcggtaaatt
     4141 ttcgggaatc catcattttc cttttcaaat gggttttgcg ccctattatc gccgcaatgc
     4201 cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca aatcgtaccg
     4261 ctgccttgcg ctttcggttt ggcggcacta aattccagtt tgctcaaggc ggaaaggtag
     4321 gctttggcgg tagcgaccaa aacgtcggta tccgcgccct gaccgttgac gacgcggttg
     4381 ccgcgcgcca aacggacgct ggtttcgcct tggctttccg taccttgcgt gacggcattg
     4441 acggaataaa tctgcaaagc cgcgccgctt tgcgccacgc tttcaatcgc tttgaaaatc
     4501 gcatccacgg ggccggagcc tgtggcggaa gcgcgttttt cttcgccttt gatgctgaac
     4561 acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga gatgaatttg
     4621 tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag gtcttcatcg
     4681 aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc gttcagtgcc
     4741 tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc gttgcggccg
     4801 gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc ggacataatc
     4861 tcgtaagttt cgcggtgttt cagcacccca tcctgatgga tgcccgattc atgcgaaaag
     4921 gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat ggtggacacc
     4981 agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc gaacaagtcg
     5041 tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc acgttcgccc
     5101 aagccgttga cagtacattc cacctgacgc gcgccgcctt ttaatgcggc aagcgaattg
     5161 gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt gccgccgttg
     5221 ggcgttttgg caatcagttc gcggaaaaat tcttcggttt tgtacgggat ggaatagccg
     5281 acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa tcaccgcgcc gcagatttcg
     5341 gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac atcgtcggtg
     5401 tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt cggcttcatc
     5461 ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat gcgttttttc
     5521 ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc acgggacaat
     5581 gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc gaaatcgccc
     5641 gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc cagctggcgg
     5701 gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgattgttc gccgtcgcgc
     5761 agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct ctccagagat
     5821 tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgattc ggcaagcgcg
     5881 gtcagctttt gcagttgcgc caaaggcagc gacccgcgcg tgcgccattt atagatggcg
     5941 gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag cgcgttcacg
     6001 ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct tgtctaaaat
     6061 aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa aacggcaata
     6121 taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac attttgtcca
     6181 ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca aattcggcgg
     6241 cacaaggtgg caacgagaca ggacaaacca acaaggacgg aaacaatgaa atacaaaaga
     6301 atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct ctcgcgcggc
     6361 aaaatccaaa ccatcacctg ccagcttgcc gcattgcatc atgcgggaca cgagctggtc
     6421 ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa aaaacgtccg
     6481 gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct gatggaagaa
     6541 tatacggcaa acctgtcttc agacggcatc gtgtccgcgc aaatcctgct cagccgcgcc
     6601 gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt gctgctgcaa
     6661 cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga attgaaaatc
     6721 ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga cctcttggtg
     6781 ctgctgaccg acatagacgg tctttacacg ggcaacccga acagcaatcc cgatgccgta
     6841 cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg cggctcgggt
     6901 tcggcaaacg gcacaggcgg tatgctgacc aaaatcaaag cggcgacgat tgcgaccgag
     6961 tccggcgtac cggtctatat ctgttcctcg ctcaaacccg atgcacttgc cgaagcggca
     7021 gataatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg gacgcagaag
     7081 caatggctgg cgttctattc cgaaagccgg ggcggcgttt atgtggacga aggtgcggaa
     7141 cacgctttgt ccgaacaggg aaaaagcctg ctgatgtcgg gcattgccag aatcgaaggg
     7201 catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca gcccctgggc
     7261 aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc gcgtaaggcg
     7321 aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat acgcctgctt
     7381 ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct tgcccttgcc
     7441 aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg cgcgctgctt
     7501 gctatggcgg acagcttgga agcggcggcg gcagatattt tggcggcaaa ccgtcaagac
     7561 ttggaagccg cggcaggcaa cattcccgaa agcatgaccg accgcctttt gttggacgaa
     7621 aaacgcattg gcgcgatggc ggacggcatc agggcggttg ccgcgctgcc cgaccccgtg
     7681 ggcgaaatac tggaaacctc gactttgccc aacggcttgg aaatcgtcaa aaagcgcgtg
     7741 gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacttc cgatgcggcg
     7801 gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga tgcattccaa
     7861 tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg catcgacccc
     7921 gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat gatgagggcg
     7981 aaagattatc tagatctgct gattccgcgc ggcggggcgg ggctgatacg ggcggtggtt
     8041 gagaatgccg tcgtgccggt cattgaaacg ggaacgggca ttgtccacat ttatatcgac
     8101 aaggacgctg attgggataa ggcactccgt atcgtgtaca acgccaaaac cagccgtccg
     8161 tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc cgacttcctg
     8221 cccaagctcg aacgactgtt ggttcgcgac cgcatagaag ccggactgcc gcccgtccgc
     8281 ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg tgcagacgat
     8341 ttcgataccg agtttttaga ctacatcctc gccgtgaaaa ccgtcgcatc ggtcgaagag
     8401 gcggtcgggc acatcgaagc ccacggcaca caccattcag acggcatcgt taccgaaaac
     8461 cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta tgtcaacgcg
     8521 tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg catctccacg
     8581 caaaaactcc acgcccgggg cccgatgggt ttgaaagagc tgacgagtta caaatacatc
     8641 gtacaaggca cgggacaggt cagggaataa tccgaattca aatgtctgat atagtggatt
     8701 aactttaaac cggtacggcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtt
     8761 ctgaggcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt cgtcgccttg
     8821 tcctgatttt tgttaatcca ctataaaaat gccgtctgaa acagtcttca gacggcattt
     8881 ccatttgcaa aaaatcaagc attgtccaag gggacgagaa tcgtacggtt gccgttgtgt
     8941 tccggtgcgg acacaatgcc ttccgcctcc atctggtcaa tcagacgcgc ggcgcggttg
     9001 tagccgatac gcaaggcgcg ctgtacgccc gaaatgctgg ctttgcgcgt tttcaggaca
     9061 acggatacgg cctcgtcgta catcggatcg gtttcgtcgt cgccgctgcg cccgatgccg
     9121 ggcagctctt cgctgccgcc gccgctcaaa atatcgtcaa catagtccgg ttcgccaaac
     9181 tgcttcagat attcgaccac gcggtgcacc tcttcatccg aggcaaacgc gccgtgaacg
     9241 cgctgcggat aggccgtacc cggcggcagg aacagcatat cgccctgacc gagcaggttt
     9301 tccgcgccca tttggtcgag aatcgtgcgg ctgtcgattt tgctggatac ttgaaacgcg
     9361 atacgcgtcg ggatgtttgc cttaatcaga cccgtgatga catcgacgct ggggcgttgc
     9421 gtggcaagaa tcaaatggat gcctgccgcg cgggcttttt gggcgaggcg ggcaatcagt
     9481 tcttcgattt tcttgcctgc cgtcatcatc aggtcggcaa actcatcaac cacgaccacg
     9541 ataaacggca atttttccaa aggttcggga ttgtcgggcg tgaggctgaa cgggttgccg
     9601 attttctccc cccttgctgc ggcttcggcg attttttgat tgaaacccgc aagattgcgc
     9661 acgcccataa agctcatcag gcggtagcgt ttttccattt cgttaacaca ccagttcagc
     9721 gcgtttgccg ccagcttcat atcggtaacg acaggggcga gcaggtgcgg gatgccttcg
     9781 taaatgctca attccagcat tttcggatcg atcataatca tacgcacgtc ttccggcgcg
     9841 gctttgaaaa gcatagacag aatcatcgcg ttgacaccca ccgatttgcc cgaaccggtc
     9901 gtgccggcaa ccaacaaatg cggcgctttt cccaagtcgg ttacgacggg ctgtccggtg
     9961 atgtcctgac cgagcgcgag cgtcagcttg gatttggatt cggcaaactc gggtgaattg
    10021 aagatttcgc tcaggcgtat catttggcgt ttcgggttcg gaagttccaa acccatacag
    10081 gttttgccgg ggatggtttc gacaacgcgg atggaagcca cgccgagcga acgcgccaag
    10141 tctttttcca gattcagaac ggaattgccg cgcacgccga catcgggttc gatttcataa
    10201 cgcgtaatca cggggccgga ataagaatcg acaaccttga ccttgacttt gaactccgcc
    10261 aatttttctt cgatggtgat gctgttttcc aacagttctt cttcggtttg cgtcgccccg
    10321 ggattgaaca gcggcggcag aagcaggtcg gttgtcggca ggtgttcgta taccgcatcg
    10381 gtttcttccg attggaacgc cccttcatcc gcttccgtat ccgatacccg gcaggacggg
    10441 cgttcagacg gcacatcttc aaacggacaa accgcccgac tgtcatgccc gaacgcttcg
    10501 gtttccgaca gatattgccc gcttgaccgc tctgccgcac cctcactgcc gtcatccgca
    10561 atagcggcgg tttcctcctg ccaacctcca ttcaaaacat catcggcaag atggtcggtt
    10621 tcggcaatgc ggctgcgttg cgcctgctcg aatcctgacg gcggttcata ggtacggttg
    10681 tagatttccg atacgggagg cggcggcgga atatcgattg cgggcatggg aactttcggc
    10741 acttcgggtg gttcgaccac ccaagcatcg ggcgcggcag gttcttcaat atggatatcg
    10801 gcagtttcat cataaaccgg accgccgtat cccacagacg aaacggtttc cgtgaaaaca
    10861 ttttcggttt cagacggcat ttcgacatcc cgtttcccga caaccgtccg actttccgga
    10921 atcaggcgcg aaatccgcgc ctccgcagaa accgttgcct tgttcggttc tttcaaattg
    10981 acggaaacgc ggcggcgggc atcgcggacg gctgtgccgt ctgaaacgga ctgtccttgg
    11041 gaaacatccg gggattttgc ctccgcctgc cctgcatgac ggcggaacgg cgcattccgt
    11101 gcattttcgg ctttgactgc agaaaaggca gacggatgct gcttctcaaa atacggctca
    11161 aatccgtaat ccgcagactc ggaaaacgct tctttgtccg catcgaacat acgggaatac
    11221 gtgcgttgaa gcacagggtc gtccaaaccg ataatctgca gcccttccat cggggtatcg
    11281 gacacgcgga ctttggggac cgctgtttcg tttttctcaa atgcatcgat atagcgtttt
    11341 ttcgtttccc ttaaagccgc gccgtttaag gcacgcgttg cttcttccaa agtgatttcc
    11401 ttaaaaaccg gacggacggg cgaaatttcg ctttcagacg gcatcaaccc ttcagcaata
    11461 ctccggtcga atgggatgcg gcggttgtcg attacggcag ttgcaatgtc ttccgtatcg
    11521 gcagcctctt cctccgcagc ttctgctgct tccgtttccg cttcttccgt cccgtttcca
    11581 ctgtctgaat atccgtcgga tgcgttcgga acatccgcac ggtttgcaga agcaggcgga
    11641 acaggcctat ccggttcgga ttcgtgcgcc agatagtttc ggcagaaacg gacgacaccg
    11701 acgaacagcc aatacactgc cgttttgacg gcatggaaaa gcatcagggc aagttcggga
    11761 aaatcgggca taccgtcttt gatttcaggc agctccgcct gtttttcccc tttcttttcc
    11821 tgccacgcag aaacctcgcg catccactcg cgttcggatt gtgcgcggac aaaaaacagg
    11881 ccggcaagcg caagcaacaa aataacgatc aaaactatcc aaaacatatg ccgtttcccg
    11941 caagacaaac gattaaacgg gtttatttta acgtgtttcc atcccaaaaa aaacaaccag
    12001 ccggcaattc tgccgaaacc gattcggctt tcggtttgcc cccgccgaca gtataatagc
    12061 cgtcccttcc cttaatcttt gcggcattgc catgattttc caaacagtct ggttttcaga
    12121 tatggtactg tcggtttcgt ggattgtcct tatcctgata ctggcagctt ccgcgccgtc
    12181 cgcattccgc tcgcttgacc gctaccgttc cgcccttcca ctgtgtaccg taattttttc
    12241 cgcagcatgg tgtctgaacg cctctgccgg cggcggacag cttgcacaga tgaactacca
    12301 cctgctcgcc gtcaatctgg ttgcactgat ggtgggcact tccgccgccc tttggcttgc
    12361 cgcgctgctg atgctgccct actgcctgct gtttgccggt tctgtcggcg catatccgcc
    12421 gaacgctttg gtgctgattc tgcccgcgct ggtcgtcaac cgcctgtcgc gtatgctggt
    12481 caaccgcctg ccgcccaata ttttcatctt tattttcgtc aacggctttc tcgcttccgc
    12541 cgccggcatt ctgctgaccg ggctggtgct gaccggtatt ttggatgccg caaacgcttt
    12601 tccgtccgaa atattgtgga cgaccgccct gcccgtcttt attctgctgg cgtgggcgga
    12661 agccttcctc agcggcattt caactgccat atttgttgca ctgaagcccc attggatcaa
    12721 cactttcgac gacaaccgct atctgaagtc cgaccgtagc atatggaggt agccttcaga
    12781 cggcataggc agacaaaacc atgctttcca acatcatccc cctttccatc ggtgcggcac
    12841 tcggtgcaac ggcgcgttgg ctgctcaacc tcgccgttcc tgccgccatg tctcccgcca
    12901 cgggcaacct gtttgccaac tggacgggcg cgctgctcat cggcatcttt gccgaaaccg
    12961 tcaatcaccc gcaatggaag ctgctgctga ttaccggttt tctcggcagc ctgaccacgc
    13021 tttccggatt ttcactggaa accgtaaccc tgctccaatc gaatcgtccc gcttcagcac
    13081 tttccaatat tttcctacac acggcaggtt cgctgctgct gacttggctc gggctgaaga
    13141 tagggacggc agtcaaataa tcaaaaaaat tgcttccttt ggttttgaca caaactatac
    13201 aagtcctaac atcttaatca tggcgcagca tataagcgtg gatttgcagc ccaatctata
    13261 ctttctgatt ctattcatta agccattatg cttattcctc aatcacggtt tccaccactg
    13321 aatgaatact tttaggcaat tgcaaaaaag cattccaagc aattaaagta gttttaaaag
    13381 tatgcagcga acagcttaaa tcaggccagc cgtcttcttc atcaaattcg ttgt
//
LOCUS       DAWFJW010000053        13272 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFJW010000053 DAWFJW010000000
VERSION     DAWFJW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.053"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            286..1516
                     /locus_tag="WGR19_001205"
                     /pseudo
     CDS             286..1516
                     /locus_tag="WGR19_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            1555..2682
                     /gene="pheA"
                     /locus_tag="WGR19_001206"
     CDS             1555..2682
                     /gene="pheA"
                     /locus_tag="WGR19_001206"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0798741.1"
                     /translation="MLECTINYHSDKIMSHHIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            2716..3507
                     /gene="recO"
                     /locus_tag="WGR19_001207"
     CDS             2716..3507
                     /gene="recO"
                     /locus_tag="WGR19_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0798742.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            3531..4259
                     /gene="pdxJ"
                     /locus_tag="WGR19_001208"
     CDS             3531..4259
                     /gene="pdxJ"
                     /locus_tag="WGR19_001208"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0798743.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            4305..4412
                     /locus_tag="WGR19_001209"
                     /pseudo
     CDS             4305..4412
                     /locus_tag="WGR19_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4804..5343
                     /locus_tag="WGR19_001210"
                     /pseudo
     CDS             4804..5343
                     /locus_tag="WGR19_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
     gene            5353..6120
                     /locus_tag="WGR19_001211"
     CDS             5353..6120
                     /locus_tag="WGR19_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0798744.1"
                     /translation="MQEIIQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            6320..6697
                     /locus_tag="WGR19_001212"
     CDS             6320..6697
                     /locus_tag="WGR19_001212"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0798745.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            6779..7588
                     /locus_tag="WGR19_001213"
     CDS             6779..7588
                     /locus_tag="WGR19_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0798746.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            7581..8003
                     /locus_tag="WGR19_001214"
     CDS             7581..8003
                     /locus_tag="WGR19_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798747.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            8052..9194
                     /locus_tag="WGR19_001215"
     CDS             8052..9194
                     /locus_tag="WGR19_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0798748.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(9495..10313)
                     /locus_tag="WGR19_001216"
     CDS             complement(9495..10313)
                     /locus_tag="WGR19_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0798749.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(10393..11643)
                     /locus_tag="WGR19_001217"
     CDS             complement(10393..11643)
                     /locus_tag="WGR19_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0798750.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPSRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKQG"
     gene            complement(11640..12101)
                     /gene="tsaE"
                     /locus_tag="WGR19_001218"
     CDS             complement(11640..12101)
                     /gene="tsaE"
                     /locus_tag="WGR19_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0798751.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            12288..13100
                     /locus_tag="WGR19_001219"
     CDS             12288..13100
                     /locus_tag="WGR19_001219"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0798752.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT         3114 a         3377 c         3653 g         3128 t
ORIGIN      
        1 atagtggatt aacaaaaatc aggacaagac gacgaagccg cagacagtac agatagtacg
       61 gaaccgattc acttggtgct taagcacctt agagaatcgt tctctttgag ctaaggcaag
      121 gcaacgctgt actggttttt gttaatccac tatatcgttc cggttcgtcc ggttttgccg
      181 gggcttttgt tgccgcctgt ttgtgccggt gtgttaaaat tttccgtttc cgcgtattgt
      241 gttttccgcc gccgggcggt ttgtttgcga atcggacgag aatttatgcc ttctgcccat
      301 tatcctgaaa tgagcgaaaa actgatggcg gttttgatgg cgatgctggt tacgctgatg
      361 ccgttttcca tcgatgccta cctgcccgcg attcccgaaa tggcgcagtc gctgaacgcg
      421 gatgtccacc gcatcgaaca gagcctgagt ttgtttatgt tcggcacggc gttcggacag
      481 gtggtcggcg gttcggtgtc cgacatcaaa gggcgcaaac ccgtctgatt gtatattgcc
      541 ttgccgttgc cgccatcgta tttgcttcga gtgccgaaca gctcctcaac ctgcgcgtcg
      601 tgcaggcatt cggtgcgggc atgactgtgg tcatcgtcgg cgcaatggtg cgcgattatt
      661 attccggacg caaagccgcc caaatgtttg cccttatcgg catcattttg atggttgtgc
      721 cgctggtcgc acccatggtc ggcgcattgt tgcagggttt gggtggctgg caggcgattt
      781 ttgtttttct ggcggcgtat tcgctggtgc tgctcggttt ggtacagtat ttcctgccca
      841 agcctgccgt cggcggcaaa atcggcaggg atgtgttcgg gctggtggct gggcggttca
      901 aacgcgtatt gaaaacccgt gccgcgatgg gttatctgtt ttttcaggca ttcagcttcg
      961 gttcgatgtt cgcctttctg accgaatctt ccttcgtgta ccagcagctt taccgtgtta
     1021 cgcctcatca atacgcttgg gcgtttgcac tcaacatcat cacgatgatg tttttcaacc
     1081 gcgttaccgc cgcgttaccg cgtggcggct caaaaccggc gtgcatccgc aaagcatcct
     1141 gctatggggg attgtcgtcc agtttgccgc caacctgtcc caactcgccg ccgtgctgtt
     1201 tttcgggttg cccccgtttt ggctgctggt cgcgtgcgtg atgttttccg tcggtacgca
     1261 gggcttggtc ggtgcaaaca cgcaggcgtg ttttatgtcc tatttcaaag aagagggcgg
     1321 cagcgcaaac gccgtattgg gtgtattcca atctttaatc ggcgcggggg tgggtatggc
     1381 ggcgaccttc ttgcacgacg gttcggcaac cgtgatggcg gcaacgatga ccgcgtccac
     1441 ctcttgcggc attgcgcttc tgtggctgtg ttcgcaccgg gcgtggaagg aaaacgaaca
     1501 gagcgaatac ctttaacgga aaatgccgtc tgaaaccgtt tcagacggca tttgatgtta
     1561 gaatgcacga taaattacca ttcagacaaa atcatgtccc accacatcga cgaactcctc
     1621 cttccccacc gcaacgccat cgacaccatc gatgccgaaa tcctgcgcct gctcaacgaa
     1681 cgtgcgcaac acgcccacgc catcggcgag ctgaaaggca cgggcgcagt gtaccgcccc
     1741 gaacgcgaag tcgccgtgtt gcgccgcatt caggatttga acaaaggccc gctgcccgac
     1801 gaatcggtag cacgcctgtt tcgggaagtg atgagcgagt gcctcgccgt agaacgcccg
     1861 ctgaccatcg cctatctggg gccgcagggc acgtttaccc agcaggcggc gatcaagcat
     1921 ttcggacacg ccgcgcatac catggcgtgt ccgaccatag acaactgctt caagcaggtt
     1981 gaaacccgtc aggcggatta tctggtcgcg cccgtggaaa actcgaccga aggctcggtc
     2041 gggcgcacgt tggatttgct tgccgttacc gcgttgcagg cgtgcggcga agtcgttttg
     2101 cgcatccacc acaacctttt gcgtaaaaac aacggcagca ccgaaggcat tgccaaagtc
     2161 ttttcccacg cgcaggcgtt ggcgcagtgc aacgactggt tgggcagaca cctgcccaac
     2221 gccgaacgga ttgccgtgtc cagcaatgcc gaagccgcaa ggctggttgc cgaatcggac
     2281 gacggtacgg ttgccgccat cgccggacgc acggcggcgg aaatctacgg actcgatatg
     2341 gttgccgagt gcatcgaaga cgaaccgaac aacaccacgc gcttcttggt gatgggacat
     2401 cacgaaaccg gtgcaagcgg cagcgacaag acttcgctgg ccgtttccgc gcccaaccgg
     2461 gcaggcgcgg ttgcctcgct gctgcaaccg ctgactgagt cgggtatttc catgaccaag
     2521 tttgagagcc gtccgagtaa atccgttttg tgggaatacc tgttcttcat cgacatcgaa
     2581 ggacaccgcc gggacgcgca gattcagacg gcattggaac gcttgggcga acgcgcttcg
     2641 ttcgtcaaag tcatcggttc gtacccgacc gccgttttgt agcggcggca gcgttcagac
     2701 ggcatttcag gcatgatgtc cgaacaccga gtcaaccacg aacccgtctt cctgttggct
     2761 tcctcgccct ggcgcgaaag cagcctgtgg gttgaagcat tcagccgccg ttacgggcgt
     2821 gtggcacttt tggcaagaag cgcgcgcaaa cggcagagcg aattgcgcgg cgtgttggtg
     2881 ccgtttgtgc cggtgagtgc gtcttggtat ggttcgcaag agttgaagac cctacatcgc
     2941 gccgaatgga taggcgggtg gccgcagccg cagggtaggg ctttgttcag cggattgtat
     3001 gtgaacgagt tgatgctgaa gttgaccgtc cgcgaagatc cgttgcccga gttatacgac
     3061 gcgttggcgg aagtgatgga ggcggtgtgc tgcaaagccg cttatatcga cgacttgcgc
     3121 cgtttcgagt ggcggctgct gaacctgttg ggcgttgccc ccgatttgaa ccgcgacggg
     3181 gacggcggga cgattgcggc aggcggcaca taccttgtcc gcccggaaac agccgtcttc
     3241 cccgtcggaa aaggatttgc cgtaccgccg cacgccgccg gcgttgtcgc ccccgggcag
     3301 agcctgatcg atttgcgcga aggcagtttc cgcactgccg aaagcctgca acaggcattg
     3361 aaaatcacac ggctttttat ccgccacctg ttgcccgagg ggctgaaatc gcggcaggtg
     3421 ttggaacaga tacggcagtt tgaccgcaaa gaaaccgccc gggaaaccgt cccgacttcg
     3481 gacggcacgg cttcaaatgc cgtctgaagg cagaaataaa aggaaagatt atgcttttag
     3541 gtgtcaacat cgaccacatc gctaccgtcc gcaatgcgcg cggcacgact tatcccagcc
     3601 ccgtggaggc ggcactggtt gccgaaacgc acggtgcgga tttgattacc atgcacctgc
     3661 gcgaagaccg ccgccacatc aaagacgcgg atgtgtttgc cgtcaaaaac gccatccgca
     3721 cgcgcctgaa ccttgaaatg gcgttgacgg aagaaatgct cgaaaacgcg cttaaagtga
     3781 tgccggaaga cgtgtgcatc gtgcctgaaa aacggcagga aatcacgacc gaaggcggct
     3841 tggacgtatt ggcgcaacag gaaaaaatcg ccgggttcac caaaatcctg accgacgcag
     3901 gcatccgagt ctctctcttt atcgatgccg acgacaggca aatccaagcc gcctatgatg
     3961 tcggcgcgcc cgttgtcgag ctgcacacag gcgcgtatgc cgacgcgcgc agccacgccg
     4021 aacaactcaa gcaattcgag cgtctgcaaa acggcgcgca ttttgccggc gatttgggct
     4081 tggtcgtcaa cgccggacac ggactgacca tacacaacgt tacccccatc gcccaaatcc
     4141 tcgccatccg cgaactgaac atcgggcatt cgctgattgc ccaagccctc ttcctcggac
     4201 tgcccgaagc cgtgcgccaa atgaaggagg cgatgttcag ggcaaggctg ctgccgtaag
     4261 ggcaggcaaa ccctttcaga cagcatttca cgacagggat atgttatagt ggattaaatt
     4321 taaatcagga caaggcggcg aagccgcaga cagtacaaat agtacggcaa ggcaagccaa
     4381 cgccgtactg gtttaaattt aattcactat atgaatcaaa agtatatttt atctgcaaac
     4441 aataatagtt tgatagaaga aattcacaat acagtacaga gtattgggta ttgtattgtt
     4501 cgaggtctta atctaaacca tcttgatggc agccggagaa acaagaaatt atttgacttt
     4561 ctatctcaat taggaatgct gacaaaccac aaaggcgatg gttttaaatc tatattttgg
     4621 gatattaaat attgaggcga tgattatgta atatagtgga ttaacaaaaa tcaggacaag
     4681 gcgacgaagc tgcagacagt acagatagta cggaaccgat tcacttggtg cttgagcacc
     4741 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
     4801 actataaata atgatataac tttctcggaa gatgttggag aatgtccact tcatagtgat
     4861 tcatctttta gtgaaaaccc ggaaagttat ttggttatgt atgtagtaaa atcagccaat
     4921 gatggaggta attccctatt tttaagttca tcagatattg tcaatcagtt atctaaaaca
     4981 gaaaccggta aaaaacactt aaaaacatta acgggcaatt tatatccatt taaaacacca
     5041 gcatcatttg ataaaaaaca aggtgtgaga tggggtaata tcttatcggt caatactcaa
     5101 atgattagat ttagaagtga ttgtatctat aaaggtattg aagaaaatag aaataaagta
     5161 tcaaaggaaa tggtacttgc acttgattat cttgtaaatg ttataaaaaa tgcgagtgat
     5221 attcaagaat tttctgcaca agatgatggt ttgattatta ttgacaatgt caatggcttg
     5281 catgccagaa ctgattatac ggataaaaac aggcattata ttagagcaag aattactgta
     5341 taaaggacgg ttatgcaaga aataatacaa tctattgttt ttgttgctgc cgcaatactg
     5401 cacggaatta caggcatggg atttccgatg ctcggtacaa ccgcattggc ttttaacatg
     5461 ccattgtcta aggttgttgc cttggtggca ttaccaagcc tgttaatgag cttgttggtt
     5521 ctatgcagca ataacaaaaa aggtttttgg caagagattg tttattattt aaaaacctat
     5581 aaattgcttg ctatcggcag cgtcgttggc agcattttgg gggtgaagtt gcttttgata
     5641 cttccagtgt cttggctgct tttactgatg gcaatcatta cattgtatta ttctgtcaat
     5701 ggtattttaa atgtatgtgc aaaagcaaaa aatattcaag tagttgccaa taataagaat
     5761 atggttcttt ttgggttttt ggcaggcatc atcggcggtt caaccaatgc catgtctccc
     5821 atattgttaa tatttttgct tagcgaaaca gaaaataaaa atcgtatcgt aaaatcaagc
     5881 aatctatgct atcttttggc gaaaattgtt caaatatata tgctaagaga ccagtattgg
     5941 ttattaaata agagtgaata cggtttaata tttttactgt ccgtattgtc tgttattgga
     6001 ttgtatgttg gaattcggtt aaggactaag attagcccaa atttttttaa aatgttaatt
     6061 tttattgttt tattggtatt ggctctgaaa atcgggcatt cgggtttaat caaactttaa
     6121 ttcattatta aatgccttaa ctccttatta aataatttgc acgatgtttt agaatttcaa
     6181 atgcaaaagg ttacagtgaa aattgttacc gacaaaaccc caaaagtgga tattcacgcc
     6241 attttaacgc cccaagaaat tgacggcatt catcatcaca ttcatcacta cccgcaacca
     6301 agggcgaagg agcgcaaata tgatttacgg catcggcaca gacattgttt ccctcaagcg
     6361 catcatccgc ttaaacaaaa aattcggaca ggcgtttgcc gggcgcatcc tcactccgga
     6421 agagctgctt gaatttccgc aagcgggcaa acccgtcaac tacctcgcca aacgctttgc
     6481 cgccaaagaa gccttcgcca aagccgtcgg cacgggcata cgcggcgcgg tttccttccg
     6541 caacatcggc atcgggcatg acgcattggg caagcccgaa tttttctacg gccccgccct
     6601 gtccaaatgg ctggaggaac aaggcatcag ccgcgtcagc ctcagcatga gcgacgaaga
     6661 agacaccgta ttggcgtttg tcgttgccga aaaataatgc cgtctgaaat gcggcaaacc
     6721 cgttgacggc attgctcgtc cctcatttgc actccgaccg accaaccgcg tacccgccat
     6781 gattcaagac acccgacccc ttatccgcgt cgttgccggc atcctgctcg attcagacgg
     6841 caactacctg ctcagctcgc gccccgaagg caaaccctat gccggatatt gggaatttgc
     6901 cggcggcaag gtcgaagcgg gcgaaaccga cttccaagcc ttgcaacgcg agtttgaaga
     6961 agaactcggc atccgcatcc ttgccgccac gccttggctg accaaaatcc attcctacga
     7021 acacgcccgc gtctgcctga aattcctatg ggtcaacccc ggccaatgga cgggcgaacc
     7081 gcaatcccgc gaagggcagg aatggtcttg gcagaaggcg ggtgatttta ccgttgcccc
     7141 catgctgccc gccaacggcg cgcttttgcg ttcgctgtcc gttccgcgcc gtttgtacgg
     7201 cagcctgaaa acgggtttgc acggggagaa cagtatgggc gcgtaccgcg tcctgccttt
     7261 gggttcggca gagggaagcg gtgcgaacgt tttgatggag gcggcgcaat ggcaggacag
     7321 acccgaacac gccgacagcg tgtggatgat ggtgcagacc cgcgaacaat ggcggcaggc
     7381 gcaggaaaag ggcgcggatg ttgtcgtgtg gcgcgtgtgc aatgatgttc aggcacaaga
     7441 ggcggcagaa gccctgcggc agggcgtatc cgtgccgctc gtacttgcag caaacggaca
     7501 gacggttgca cgttatggaa aactatggct cggattgggg gcgcacgtgg tggtaaggga
     7561 tgaaacaata gggaagaatc atgaataaaa accgtaaatt actgcttgcc gcactgctgc
     7621 tgattgcctt tgccgccgtc aagctcgttt tgttgcaatg gtggcaggcg cagcagccgc
     7681 aagctgtggc ggcgcaatgc gatttgaccg agggttgcac gctgccggac ggaagccgcg
     7741 tccgcgccgc cgccgtttca accaaaaaac cgtttgatat ttatatcgaa cacgcgcccg
     7801 ccggcacgga acaggtcagc atcagcttca gtatgaaaaa tatggatatg ggtttcaacc
     7861 gctatatgtt cgagcggcaa ccgtcgggga cttggcaggc agtacgcatc cgcctgccca
     7921 tctgtgtcga aggcaggcgc gattttacgg cggacattac aatcggcagt cggacatttc
     7981 agacggcatt taccgccgaa taaacctttc aatccgccat tgccggaaca tccgtccgga
     8041 aaggacacgt tatgaatact ttatatacac ttttcgccac ctgcccgcgc ggcttggaga
     8101 ccgttttatc tcaagaactc gaaagcctcg gctgtaccga tgtacaagtg tttgacggcg
     8161 gcgtttcctg ccggggcgga ttggaacagg tttacgccgc caacctgcac tcgcgtaccg
     8221 ccggccgcat cctgctacgc ctgaccaaag gcacataccg aaccgagcgc gacatttaca
     8281 aacttgccaa aaacatcaac tggttcaact ggtttacctt gcagcagacg ttcaaagtca
     8341 aaatcgaggc aaagcgtgcc aacgtcaaaa gcctccaatt tgtcggactg accgtcaaag
     8401 atgccgtctg cgacgctttc cgcgacattt acgacgcacg tccgagcgtg gacaaagccg
     8461 cgcccgatgt tcgcatccac gcctttttgg acgaacgcaa tgtcgaaatc ttcatcgaca
     8521 cttcgggcga agccctgttc aaacgcggct accgtctcga taccggcgaa gccccgctgc
     8581 gcgaaaacct tgccgccgga ttgctgctct cggcaggcta cgacggcacg cagccgtttc
     8641 aagacccgtt ttgcggcagc ggcacgattg ctatcgaagc cgcttggatt gccgcccgcc
     8701 gcgcgccggg tatgatgcgc cgtttcggtt ttgaaaaact gcaaaatttc gataaaacgc
     8761 tgtggtcgga tttgcgccgc cgtgccgaag cgcaggttaa accggctttt gcaccgattt
     8821 cgggcagcga cagcgaccgc cgcatcattc agacggcatt ggccaacgca cgcagtgccg
     8881 gggtggacga catcgtttcc ttcagcgttg ccgacgcgca atccgtccgt ccgaacggcg
     8941 tgggcggcat tatggtgtcc aatccgccct acggtgtgcg ccttgaggaa gtccgcgctt
     9001 tgcaggcact gtatccgcag ttggggacgt ggttgaaaaa acactatgcc ggctggcagg
     9061 tcggtatgtt taccggcgac agggaaatgc ccaaattcat gcgcctgtcg cccaagcgga
     9121 aaattccgct ttataacggc aacctcgact gccgcctgtt cctgattgat atggtgcagg
     9181 gatcgaaccg ttgaggaaag tgtacaaaaa agccgtctga aaaatgttca gacggctttt
     9241 tttaatatcc ggccgatatt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     9301 cagacagtac aaatagtacg taaccgattc acttggtgct tgagcacctt agagaatcgt
     9361 tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaattcac tataaccgaa
     9421 ggtaagggag gggtgtgtcc actgtgccgc cgcatttgcc gcgtgcggaa gatacggata
     9481 acaacccctt ggaattaaag ggagaagttg cgggaaatat ccatatcagg ctggggaccg
     9541 tacttattta cggtattttc gtatgccgaa ataaaatatt tcttgaataa accgtaatcg
     9601 ccggtttcgt aataggctac gacagcctcg gcataggcgg gataactgtc gggggagaat
     9661 acgcaaggaa aaaaaccgga gcgcatcagc gacaaggtca tgcagtttct ggcagtacgt
     9721 ttattgcaat ctttaaaata ttgaagatac gccaaattat tatggagata aacggcgcgg
     9781 tcgaagggat ttcctatttt tggggcttct tgaagcaacc attttaattc cgtatccaaa
     9841 ctttgcgggt tggataaagg cgtgtagtct gtcccgctta tggtaacgct gtcgcggcgg
     9901 actacgccgc cggagccttt ttccagtaag ttttccgaaa tcaggctgtg ggtggttttt
     9961 aagaaatcca accagtcaaa cggatcgggg ctgtccaaac cggacagcag gtgccggtaa
    10021 ctttccctca ggttgattaa cattacggca tccgaatata acttcccgcc tgcggtttgc
    10081 ccgagtttaa gcaatgcctg cgtatcgtat tgattataag tgtttccttc caatttggca
    10141 gaactgtaca caaaatcgat accgatttct tcaatattac gcacggagtc cgttaaaaca
    10201 gataaaggaa gccgatcgga caattcggtc aggtattctt gttcgccggc tgtaaaaatg
    10261 gggcggatat ttaaaaaatc ctgttggtat ctcaatttat ccataccttt catcataaaa
    10321 acgcggtttg gggcggaagt aggtttatgc ctgccttcat ccgacaacta tattgaaaca
    10381 ccgccttttc aatcaaccct gcttcaatac ggaagtgttg atgtagcgtt ggacacccga
    10441 ggcaatggat tgggcgcact gtcggcggaa ggattcgctg cccagcagct tctcttcggc
    10501 agggttggac aggaaggcgg tttcgaccag gatagacggc atatcgggtg cgcgcaaaac
    10561 ggcgaaattg gcttcgtcca ccctgccttt gtgcagatgg ttgagcctgc ccaattcttc
    10621 aagcaccagt ttgccgagtt tgcggctgtc gcgcagcgtg gcggtttggg tcatgtcgag
    10681 cagggcggta tcgacattgc ggttgccgct ggtcggtacg ccgccgaccg cgtcggcatt
    10741 gttttgcgtc tgttccaaga atttggcggc ggagctggtc gcgcctttgg tgttcagcat
    10801 ataaaccccc gttccgcgcg cggaggggct ggtgaaggca tcggcgtgga tggagacaaa
    10861 tacgtccgcc cgccgtgcgc gccctttggc gacacgcacg cccaaaggga tgaacacgtc
    10921 ttcgttgcgc gtcataaaga cgttgtaacc caaggcttcc aattgttttt tggtttcgcg
    10981 ggcgatggag aggacgacgt gtttttcctg caaaccgccc gggctgacgg cgccggggtc
    11041 ttcgccgccg tgtccggggt cgatcataat gacgggtctg cgcccgtttt tgccgcgttc
    11101 ggtttgtgcg ggagtgtttt gagcgaggtc ggcttcaggg gagccgcgac gggttttgtt
    11161 caggctgccg ttaagcagcg ccatcatcgg atcgtcggga tccatccctt gcggatagag
    11221 atcgacgacg aggcggtttt taaagccgcc gacggggggg agggaaaaaa cttgcgcgta
    11281 ggaaggctgt ttcaaatcga tgacgaggcg gacggtggtc ggcgtgttct gacccgcgcg
    11341 tatgctgcgg ataaaggggt cgtccgccat aaccttctga gacagtccgt gcaatacggt
    11401 attgatgttc gcgttttgta tgtcgacgac cagcctgctt gggttgtcga gcgtgaagtg
    11461 ttggtatttg agcgcggttg tgctttccag cgtcaggcgg gtgtaggtgt gcgacggcca
    11521 tatccgtacg gcggtaaact gcggggcgcg taccgttttg gcaacggcgg atgcgatggg
    11581 gcttagggcg aacagtgtgc cggcggtgcg gcggatgatt tgtcttcgtg tcagtttggt
    11641 catagcggca ggctttcgcg tcctcgttcg gtatgggcgg tcagcaggca ttttctgccg
    11701 ccgccgtcgt gtgtcaatgt tgcggtgatg tcggcgggcg gggtaaattc gcctccctgt
    11761 tgcggccatt cgatcaggca gacgctgttt gcggcaaaca gttcgtcaag ccccgcgtct
    11821 tcccattctt cggggaacga gaagcggtag aggtcgaaat ggtgcagggt gaagcgttcc
    11881 agcggataag attcgacgat ggcgtaggtc gggcttttga ctgcgccctg atgacccaat
    11941 ccgcgcagga tgccgcgtgt cagcgtggtt ttgcccgcac ccaaatcccc ttcgagataa
    12001 atgaccagcg gtgcgtttaa acgggaagac cacgccgcac ccaaatcgag tgtggcggct
    12061 tcgtcggcaa ggaatcggga aacgggagga aaatcagaca taggggcttt acaactttta
    12121 tgtggcggat cggaggggca ggcggtcggc aaaccgtgcg cccggcggca ttgccgtata
    12181 ttataacagc caaatccgca tcctgctttc agacggcaac ggctgtcaag aaaaagcggc
    12241 gcgtgtacaa tacgcggatt gtatgtttag gacggattgg aaaaagaatg gaaaatatcg
    12301 gcaggcagcg acccatcggc gtttttgact cgggaatcgg cggtttgacc aatgtgcgag
    12361 cgctgatgga acggctaccg atggagaaca tcatttattt cggcgacacg gcgcgcgtgc
    12421 cttacgggac gaaatctaag gcgaccatcg aaaatttctc gatgcagatt gtcgattttt
    12481 tattggaaca cgatgtcaag gcgatggtta tcgcgtgcaa tacgattgcg gcggtggcgg
    12541 ggcagaaaat ccgtcaaaaa accggcaata tgcccgtttt ggacgtgatt tccgccggcg
    12601 cgaaagccgc gctggcaacg acgcgcaaca ataaaatcgg cattatcgcc accaatacga
    12661 cagtcaacag caacgcctat gcgcgcgcca tccacaggaa caaccccgat acgctcgtcc
    12721 gcacgcaggc cgcgccgctg ctcgtccctt tggtggagga aggttggttg gaacatgaag
    12781 ttacccgcct gaccgtatgc gaatacctca aaccattgct tgcggacggc atcgatacgc
    12841 tggtgttggg ctgcacgcac tttcccttgc tcaaaccctt aatcggcagg gaggcgcaca
    12901 atgtcgcact ggtcgattcc gcgattacga ctgccgaaga aaccgcacgc gtccttgcgc
    12961 aggagggatt actcaatacc gacaacaaca atcccgacta ccgtttttat gtcagcgata
    13021 ttcctttgaa attcagaacc atcggcgagc gttttctggg caggacgatg gagcagattg
    13081 aaatggtgtc tttgggttag cggcaggtac ggacggaaac cgtccccgtc cgcgccatcc
    13141 gcatacgcac ccgcaacgcc ctgccgtatt aaacgccccg tcgccgcaaa tgccgtctga
    13201 aaggcttcag acggcatttt ttacacaatt cccaccgttt cccatccttc tcgatacact
    13261 gtaatctcga aa
//
LOCUS       DAWFJW010000054        13194 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFJW010000054 DAWFJW010000000
VERSION     DAWFJW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13194
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.054"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(84..890)
                     /locus_tag="WGR19_001220"
     CDS             complement(84..890)
                     /locus_tag="WGR19_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0798753.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKMELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1111..2481
                     /gene="ffh"
                     /locus_tag="WGR19_001221"
     CDS             1111..2481
                     /gene="ffh"
                     /locus_tag="WGR19_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0798754.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
     gene            complement(2549..3244)
                     /locus_tag="WGR19_001222"
     CDS             complement(2549..3244)
                     /locus_tag="WGR19_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0798755.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHTKTFKDDTYLRRE
                     HVIWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(3415..4074)
                     /locus_tag="WGR19_001223"
     CDS             complement(3415..4074)
                     /locus_tag="WGR19_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0798756.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRRNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(4277..6388)
                     /locus_tag="WGR19_001224"
     CDS             complement(4277..6388)
                     /locus_tag="WGR19_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0798757.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYNRDFSASINPYD
                     RASWPASGRLQPVFTQNRHKADAYGIFVQNIFSATPDLKFVLGGRYDKYTFNSESKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDPKNNPYIYAVSGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVIQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            complement(6574..7179)
                     /locus_tag="WGR19_001225"
     CDS             complement(6574..7179)
                     /locus_tag="WGR19_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0798758.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(7228..7626)
                     /locus_tag="WGR19_001226"
     CDS             complement(7228..7626)
                     /locus_tag="WGR19_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0798759.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            7734..8645
                     /locus_tag="WGR19_001227"
     CDS             7734..8645
                     /locus_tag="WGR19_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0798760.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IAFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            8635..9936
                     /locus_tag="WGR19_001228"
     CDS             8635..9936
                     /locus_tag="WGR19_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0798761.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(10004..10228)
                     /locus_tag="WGR19_001229"
     CDS             complement(10004..10228)
                     /locus_tag="WGR19_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798762.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLMIESEKNGRY"
     gene            10331..12481
                     /gene="dinG"
                     /locus_tag="WGR19_001230"
     CDS             10331..12481
                     /gene="dinG"
                     /locus_tag="WGR19_001230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0798763.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            12517..13131
                     /locus_tag="WGR19_001231"
     CDS             12517..13131
                     /locus_tag="WGR19_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0798764.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         3202 a         3693 c         3371 g         2928 t
ORIGIN      
        1 ttcctctccc tcctccggct ggtgcgcctt tgtgaatatg ccgtctgaaa ctcggggact
       61 cagacggcat ctgttggctc ttcttatctt ttcagaatga tttccaatac gaacttgctg
      121 cccatatagg caatcataag gctgacaaat ccgatgatgg tccacacggc ggcttttttg
      181 ccgcgccatg cggtcatgct gtgcttgagc agcagtccgc cgtaaatcag ccatgacaat
      241 atgccgaata cggttttatg ggtaaaggtc atgggtttgc cgaatacggc ttcggcaaaa
      301 aatgttccgc tgacgacgga ataggtcagc aggatgaaac ctgcccacat ggcttggaac
      361 atgagttttt ccaaactgag cagcgacggc aggaatcctg cgagcttgga gaagttcctg
      421 cggtgcaggc tccgattcag cagcagggtc aaaacggaca ataatgttgc gatgccgaac
      481 agcccgtatg cgagcagcga agttccgata tgcagcataa agggaaggtc ggtaatttca
      541 tatcccgaga attttccagg aaaaaccaaa cctgacagca gcatcagtgc ggcgcaagga
      601 tacagcagca actgcactcc gcgcagcgaa taaaagaagc tgccggcaaa ataaataaac
      661 agcatcatcc aaacaatcag gctgccggaa tacccaaagc ccataatgat gattttgtct
      721 tgaatgaccg gcataagcag tgccgcgcca tggacggtca atgccgcgcc caaaaccggc
      781 aattccatct tccacgggta atcccggccg agcccctgct gttggcagtg ccatgcaaat
      841 gcacccaatc ctgcgtaaac cgccgtcaaa aagatgaaaa ctgtcggcat ggtggacttt
      901 ctctaatcta tactgttgcg ctgtatgcgg ccgcttatga aatattggaa cttttaacgt
      961 tggaattgta aaatccccgt ttcgggcaag ccttgacgga tttgccgata tgctgtccgg
     1021 cacacaagcc gcatcaaatt attttgattt tattttaaca aagaatgccc ctgatggggc
     1081 aagctattct tattcagacc aaggaccagt atgttagaca atttaaccgg ccgcttcagc
     1141 aatgttctca aaaacatccg cgggcaggcc aaactgaccg aagacaatat taaagaagcc
     1201 ttgcgcgaag tccgcctcgc cctgcttgag gcggatgtcg ccctgcctgt cgtcaaagag
     1261 ttcgtcaaca acgtcaaaga aaaggccctc ggtcaggaag tggtgggcag cctgacgccg
     1321 gatcaggcat ttatcggcgt ggtcaacaaa gccctgaccg agctgatggg cagggaaaac
     1381 aaaacgctgg atttgtcggt tgcgccgccc gccgtcgtgt tgatggcagg tttgcagggc
     1441 gcgggcaaga cgacgaccgc cggcaaactc gcccgcctgc tgaaaaacga tcagaagaaa
     1501 aaggttttgg tcgtatctgc cgacgtttac cgccctgccg cgattgaaca actgcgtctg
     1561 ttggccgaac aggtcggcgt ggactttttc ccatccgata ccaaccaaaa accggttgag
     1621 attgcaactg ccgccgtcga ttacgccaaa aaacattttt acgatgtatt gatggtcgat
     1681 accgccggcc gtttggcaat cgatgaggag atgatgaacg aaatcaaagc ccttcacgcg
     1741 gcggttaacc cggtggaaac tttgttcgtc atcgatgcga tgctgggtca ggatgcggtg
     1801 aacactgctc aggcatttaa tgaagccctg ccgctgaccg gtgtcgtatt gaccaagatg
     1861 gacggcgact cgcgcggcgg tgcggcattg tccgtacgcc atgtgaccgg caaaccgatt
     1921 aaatttatcg gcgtcggcga gaaaatcaac ggcctcgaac ctttccaccc cgaccgtctt
     1981 gccagccgta tcctcggcat gggcgacgta ttgaccctga ttgaagacgt tcaaaaaggt
     2041 atagacgaag aagccgccgc caaaatggcg aaaaagctgc acaaaggcaa aggcttcgac
     2101 ctcaacgact ttaaagaaca aatccaacag atgcgcaata tgggcggttt ggaaaacctg
     2161 atgtcgaaaa tgccgggcga actgggtcaa atatcgaaac aaattcccga aggcacggct
     2221 gaaaaagcga tgggcaaagt ggaagccatc atcaactcga tgactccaaa agaacgcgcc
     2281 aaccctgccc tgctcaaagc cagccgcaaa cgccgtattg caatgggtgc gggcacgacc
     2341 gtgcaggaag tcaacaaatt gctcaaacag tttgaacaaa tgcaacaaat gatgaagatg
     2401 ttcagcggca acggtttggg caaactgatg cgtttagcca aaggcatgaa gggaatgaaa
     2461 ggtatgttcc caggtttgta ggccgattta acagaaaacg ccgtctgaaa tctattttca
     2521 gacggccttt actcattcaa tcctcaactt attgcggctt tttctgctct tcgcgtactt
     2581 tatccaccaa ctggtcaatc gtggtcatac cggactgcca gtctttaaat tcaacttggt
     2641 atttgccgcc gacaatcaca gtcggtgtgc cgctgatttg gaatttattg gtcaactctt
     2701 ccatttgagc cgcacgcgct tggctttcag gagcctcaaa tgcagccaat acttttttgc
     2761 cgtcaaatgc agtttgctcg gacaaccatt ttttcagggt atcggtatcg gccagattga
     2821 ttttttgatt aaccattgca tcaaaaatat ggctgttggc tttatctgat tcgccggcca
     2881 tttccactgc ggcggccaaa cgagccaaag gtttcatttc gtcaccccaa atcacatgct
     2941 cgcgacgcag gtaggtatcg tctttaaacg ttttggtgtg ttcgctcaaa accggttcca
     3001 aatgagcgca atgcgggcag aagtagccga aaaattccaa tacttcgatt ttaccggcct
     3061 gctgttgcgg aataggcgta gacaatacag tgtagttcac accttcgttc aactcagcag
     3121 gggctgccgg agcagatgag ctgctttggg cgctgtctgc cggaacactg gtttcagcct
     3181 gtttgctaca agcggccaat gccaacaggg tcaatgaagt caaagctaag gttttcagtt
     3241 tcataggtat ctcttgtgtg tcagattaat gtgcggattt tatggcattt tattgaaggc
     3301 gtgttcgatt tgagtgaaaa aatgtgttta gttttatttc agcctttata aaggccgtct
     3361 gaaatatgac ttagataatt agccttattt tcagacggcc tttgttctag taatttacgg
     3421 tttcggacat tttgtcttca atccgtcgat gtgttccatt aagtcaggtg ggacatgatt
     3481 ccacatagga tcaagaataa aatcgatacc ttcccgtctg gctaatttgg ctgctgggac
     3541 aaaatcggaa tcaccagaaa acaagatgat tttatcggct tgttttttta aggtaataga
     3601 tgaaatatcc aagcctatac gcatatctac acctttttgt ttaacatcga gcacgacatc
     3661 gttttccgta atttgagttg acgggttttt cagaagattt ttcatgactt ggggttttaa
     3721 tatccatgct gtattgtccg acagcttgcc taaacgtaac gctaatttac gcttcttctt
     3781 caattcggta tgcagattgc gacgaaatat ggcttccgga gtctttgaaa aatctatggc
     3841 acgatgagat acgggattgt gcattttctt ttcaaacggc acgcagtcgt aaaagaaaat
     3901 tctatataaa tcatccattt gatctgtgcc gttgatttta tctttcagat gcagcatcga
     3961 atatcggaaa gccaaatcag caattctttt tgcatcgtgt ggattttgag acaggcctaa
     4021 tgaccgtacc cgcttaataa aaaatgcacc gtcaatcaaa atggcggttt tcatattgct
     4081 tcccctatat ttgtcaaaga tataaaaaag cccttgggat cggcataccc gctatgattt
     4141 aatgggtaat gcgtactgcc aagggcgcta tttctttttg gattatactg atgtagataa
     4201 agcctgtcaa aaagttcgtg tgctgaattt acggattatc aggattttca gacggcattc
     4261 tgttgcacga acgggattag aaactgtaat taacccgcgc cgtataagtg cgcggcgcgc
     4321 cgggcatggc atccgaacgc caatattttt gattgagcag attggctgcg gcaaaggtaa
     4381 tgttaacatt tttatggttc cagccaagca tggcatcaac tcgggcaaag cctggaagcg
     4441 tagtcacttc tttatttctt gagttgtaac cgtagcgttt gcctgtaccg gttacgccga
     4501 tttcgccgta gaggttttcg gtcggggtat aacggaaaaa caggttgccg gtaacgttgc
     4561 tggtgttatt caaatggatg cccactcggt cgggattttc tttgtcttca acgactttcg
     4621 cctgaatcac gcccaacgaa ccgcgcagat agagtttttt ggggatgatt tgcccgatgg
     4681 cggacaattc cacgccacgc gaacggtgtt tgccgctaac cgcataaata taagggttgt
     4741 tttttggatc ggggcggtag cggatattga agcgttcgat ttggtaggcg gacaatgtgg
     4801 tgctgaggcg gtcgtccagc caactgcttt tcacgccggt ttcgtattgg cgggtgtact
     4861 cggggtcggc gttgaacacg gcggaagacg acgtatcgat gctcaaatag ccgccgcgtc
     4921 cgccataagg cgcgaagcct ttgttatacg aggcgtaaag tgtgtggact gggttgatgt
     4981 tccacactgc gccgatgttg gggctgaacg aatggccgct gtattggcgg ctgctgccgg
     5041 tgagtttgct ttcggaatta aaggtgtatt tgtcgtaacg gccgccgagg acgaatttca
     5101 agtcgggcgt ggcggagaag atgttttgca cgaaaatgcc gtaggcgtcg gctttgtgcc
     5161 ggttttgggt gaagacgggt tgcagcctgc ccgaagccgg ccagcttgcg cggtcgtagg
     5221 ggtttatgga ggcggaaaag tcgcggttgt aacctaaggt cgggttgcgg tgttcgcggc
     5281 tgtaatccat gcctacggtc aagtggtttt caaaacggcc gatggtgtag tcgccgttta
     5341 gcgtgaaatt ggacgacagg gttttgttgt cagtctgctg ccaggcgtag ttacgtttga
     5401 ttaagttgcc attttcgctg cctgcataga aatgatcaaa atcctgcgcc gccgtgcggt
     5461 gggcgagctg ccattgggca cgccatttgt cgttgaaggc gtattcaagg tcggaacgcc
     5521 aaacttgcag cttgtctttg acaaaatcgt tccggtgggc gaaccccatg cggtaaggca
     5581 gtccgaagcg gtcgtacacg gacttggtcg gactgcggtc gggcgtgcgc tccacattgt
     5641 cgtaggtgta ttgccccgtc cacttcaagc cgttgtcgag tttgacggta atgctgggcg
     5701 aaaccatgac atttttgctg tctatgccgc tgcggaacga attggcgcgc ccgacttcgc
     5761 cggtgagacg gatggcgacg tttttgttca gcacttcgtt gatgtccata ttcaggctgc
     5821 ggtttgccca cgaaccgtaa accgcaccga tgttgcggct ttgtttgaag ttggcgtatt
     5881 tgctgaccat gttgatgacg ccgccgccgt tggtgcggcc gtaaagcacg gaagacgggc
     5941 ctttcaggat ttccacgcgc tcgatgttgg cagtactgcg gcgcacttgt ccgctttcgc
     6001 gcacgccgtc gcggtaaata tcggatgcgt cggcttgaaa accgcgcagg aaaatgcttt
     6061 caccgcgcat atcgtaggca gcgtcgatgc cggcattgcc ttcgaggatg gaactcaaat
     6121 cgttcgtacc gtaatttttg tttttctgga tattgagcgt atcgatggtt tgcggcgttt
     6181 ctttgatgag ctgtccgttg cgggtaacgg cggcttcgtc gtagttgatg tagcctttga
     6241 gtacgctggt gtcggactgt ccgaccacgg aaacggtagg tagcgtggcg gtgtaatgtt
     6301 caccattgtc ctgcgtatcg gcggcagcaa cagggaagga agcaataatc agcgtgggta
     6361 ataaagctaa atgaaatgat attttcattt ttatactcaa tttaacaaaa caaccgaatt
     6421 atattgcctc acggaggaaa tgagaataat ttcttttaac tatattgaac atgatatttg
     6481 taaacaaagg tctcagaatg cggaaaactc gccgcctgat actgaaaaat gccgtctgaa
     6541 cagggttcag acggcatttt tttgaccgcg aaattatgcg ccgaacactt tcaaacgttc
     6601 tgcaacgggt tcaaaggtct tttcacctgc cgccccttca ataccgccga taaccatttg
     6661 tgcgcgcaac aaccagtttt cggggatatt ccacgctttg gcaatcgccg catcgggcaa
     6721 gggattgtaa tgttgcaggt ttgcacctac gccgaccgcg gcaagtgtcg tccaaacggc
     6781 atactgcacc atcgcgttcg cctggtccgc ccaaacggga aagttggcgg cataagcagg
     6841 gaactgctcc tgcaaacctt tgacgacatt ttgatcttca taaaacaaaa tagttgccgc
     6901 acccgcctta aacaggttca atttttgcgc ggtcggttca aaactgtcgg caggcacgac
     6961 ggcacgcagc gcgtcttcga caaattgcca caccttatca tgctcttcgc caaacagcac
     7021 gaccacacgg gcagattggg aattgaacga agaaggcgtg tgcaaaacgg cgtgttcgac
     7081 gatttggacg atttcatctt tgccgacggg cagattttta tttaacgaat aaatggaacg
     7141 gcggctttcg gcagcctgtt gcagagattg acgggtcatt tcttttcctc aaaaacattc
     7201 aacaaaatac actttaggca gtctgattca ggctttacgc ttgaaaaaca gcctgtccag
     7261 cgaccatgct ccgccgcccg ccgccgcgat atagaggaat acgaagcaga acagcactgc
     7321 ggactcgccg ccgttggcaa tcgggaacaa agcatttccg gaagcgtgcg ccataaaata
     7381 ggcaaccgcc atctggccgg acaaaacaaa cgcggcaggg cgcgcaaaca ggcccaacac
     7441 cagcaaaatg ccgccgacaa tttctaaaat accggcaagc agcaacagcc cgccgggcga
     7501 accgctgccc atttcaatgg ggaaggcgaa gattttcgac gtaccgtgca acaaaaacag
     7561 gtaggcggtt acgatacgca aaacagaaag caaaaccggt tggatacggt tgcagcaatc
     7621 ggacataaaa gctcctttga tatttgtctt catttcaggc ggtagagtat agatgtttcc
     7681 ccttttcaaa tatatactgc ttttattgat acaacttttc cacacaagaa aatatggaca
     7741 ccctgttcag cctcaaggtt ttccgccaag tcgtccaaag cggcggcttc acccgcgccg
     7801 ccgacgcgct cggcatctcc accgcaatgg caagcaaaca cgtcagccac ttggaaaaca
     7861 ccgtccaagc caaactcctg caccgcaaca gccgcaacct cagcctgacc ggagccgggg
     7921 aagaatacta ccggcaatgc agttacgcgc tcgacacgct cgacgatgcc gcgcaaaaag
     7981 ccgccggagg gacggaaaaa ccgcaggggc tgctgcgcgt aacgatgccg ctgtggtttg
     8041 ccggcagcca gatatgcaac tggctggcgg aataccgcga acgttatccc gaagtggcat
     8101 tagaactgat tttggacaac cgccacgtcg atttgattgc cgaaggcgtg gatttggcgt
     8161 tgcgcgtttc ccaaaccctg tccccgtcgc tgattgcgcg cccactggcg gaaatcgcat
     8221 ttgccctgct cgcctcgccc gatttcctga ggcgcaacgg cgtgccggaa acgccggaag
     8281 aagtggcggg gctgcccgcc gtcctgccga cctacaccaa ccagcagaaa ctcgacctca
     8341 cccgcaaatc ggacggcaaa aaataccggc ttgaactgac ccccgtcatc cgtaccgaca
     8401 acacgctgat gatgcgcgaa atgattaagg cgggcgcgtg catcggttat cagccgcttt
     8461 gggcggcgga acacgatttg cgctgcggca cgctggtgag gctgctgccc ggatacgccg
     8521 tcccgaccga ccggctgaat gccgtttatg cagacagggc attcttaagc gcgaaagtcc
     8581 gcagcttcat cgattttctg aacgaaaaaa tcgccagcag gaaaggctgc cgaaatgccg
     8641 tctgaaaccg cccgccccct ttatgcggac acgtgcgccg cacacacgct cgtctggttc
     8701 cgccaaaacc tccgcatccg cgacaacgcc gccttatgca ccgccgttgc cgaaggtttg
     8761 cccgttatcg gtatttggat tgacgatgcc gaaacagaca accctcgccg cgccgcgttc
     8821 taccgccaat ccgccgccga actcgcccaa gggcttgcag ggcgcggcat cccgctctac
     8881 acggcggcat cgcctgccga gctcgtccgg ctcgccgtcc gcctcaatat ccgcgccgtc
     8941 atcgccgacg aatcccatac ttttgccgac aaactcgccg acaacgccct ttggcatgaa
     9001 ttggacaaac acggcatcgc gttaaccttc gtcaatgacc gcgccgtttt cggcaaaacc
     9061 gacctgatac ccgacggcgg cacggcacac accgatttcg accgctaccg cgaagtatgg
     9121 ctcgaccgct tttccaagca gccccccgcc ggtccggacc tattcgcggc ataccgccaa
     9181 cccttccccg aaaacctttc cgccccgccg cctgccgccc tttcagacgg catcttcctg
     9241 ccgcaaaaca gcggcgaaac ggcggcttgg cggcagtggc ggcggtttct cgaacaggcg
     9301 gattcctact ccgttttaaa ggatttcccc tcgcgcaaac acacttcgct gatgggcgcg
     9361 tatttgagtg ccggctgcat ctcgccgcgc ctgctcgcgc gggaaagcct cgaacgccgt
     9421 ctgaacgcgt gggcggacaa catcatccgc cgcgattttt tccttcaact tgccttgcag
     9481 cacacggatg acgacccttc agacggcaat cctgaacaca ccctgcgcct gacgctttgg
     9541 cagcagggcc agaccggcat tccgattatc gatgccgcga tgcgctgttt gcacaaaacc
     9601 ggcagcctcc accccgccct gagacgcttg agcgcggatt ttttctgcca cgttttaaac
     9661 ctcccccgcc gcgaaggcga gatatggttt gcccggcagc tgaccgattt cgatgcggca
     9721 atcaaccaag gcaactggtg gcttgccgcc tcacggcaca cctgccccga cattgccgcc
     9781 gcctcatacc gtaccgaccc ggacggtact tttatcaaac ggcacattcc cgaactcgcc
     9841 cacctgtccg ccgacaccgt ccacacgcct tggcggtttg cctgttcggt cgatacccac
     9901 ggctatcccg cccatcctgt cgccggtatt ccctaagcgg tacggcaacc taaaacaaat
     9961 gccgtctgaa cttccgtttc agacggcatt gcgtccgaat gactcaatat cttccgtttt
    10021 tttcactttc aatcatcagg tatgcccaaa tcaccgccac ccaagccgac accgttccca
    10081 tcgccggcaa aaaatgctgc caatccgtca tcggcggcac agggcaacgg ccgcattcga
    10141 tttccgcgcc gcccaacaac cggtacagca acatatccgc aatccaaagc aacaacgggt
    10201 atttcaaacg ctgcaaccaa aaaaccaacc acgtccgcac gattcacctc accccccatt
    10261 ccgacaacga tattgtaccg cttccgtgca atgttaaaat aagcccactt ctaccgccgt
    10321 acaaaacgcc atgctcaccg atttagaaaa aaacgccatc cgcgaccatt accaaaacat
    10381 cggcaaaaac ctgcccggtt tccgtccgcg cgcttcgcag cgggaaatga ttgcggcggt
    10441 tgccaacgct ttttcgcgga cgttggcgcg cgaagaaggc ggcgagccgc ccaagcgcga
    10501 gggcgagagc atcgccgtaa tcgaagggcc gaccggcgtg ggcaaatcgc ttgcctacct
    10561 gctggcgggc ggcatcatgg cgcagacgcg cggcaagcgg ctgattgtga gcagcgcaac
    10621 ggttgccttg caggagcagt tggtagaccg cgacctgccg tttttggtcg aaaaaagcgg
    10681 tttggaactg agcttcgcgc ttgccaaagg gcgcggccgc tatctctgcc cctacaaact
    10741 ctatcaactg acgcaaagca atgcccagca aaacctgctc ggctttgaag cccccgccgt
    10801 cttgtgggac agcaaaccca agcccgaaga attgaagctg ctgcgcgaca tcgccgacga
    10861 attttccgcc cgacggttca acggcgaccg cgacacttgg ccggaaaaaa tcgatgacgc
    10921 gatttggctc aaagtgacca acgaccgcca cggctgcctg aaaaccgcct gtcccaaccg
    10981 tccggaatgt ccgttttacc tagcacgcga tgtcttggaa accgtcgatg tcgtcgttgc
    11041 caaccacgat cttctgcttg ccgacatcag tatgggcggc ggcgtgattc tgcctgcgcc
    11101 cgaaaacagt ttctattgca tagacgaagc gcaccacctg cccaaaaaag ccctcagccg
    11161 ttttgccgcc gaacattcat ggaatattgc cgtttggacg ctggaaaaac tgccgcagct
    11221 gaccggcaaa attgccgcgc tgaccgataa agccgaactt gccaaccttg ccgacgaagc
    11281 cgccgcatcc ttgctcgaca gcctgcacga atggcagttc catttggcgg aagagccgtc
    11341 tttaagtttg ggggtgtctg aaaacgacag acgaaccaac agcgaaccga cttggctgtg
    11401 ggaagacggc aaaatccccg aaggcctcga aaccaccgtt tccaatacgg ccattgctgc
    11461 gcgcagcctg ctcaaacacg ttgtcgggct gaacgatgcg ctttccgccg cgcgccgcga
    11521 aaaagaacag gacggcgcgc tcctcgaccg cctgaccggc gagttcggca tcttcatcgt
    11581 ccgtatcgaa caaatcagcg cggtttggga tttgctctcc accgtccccc tcgaaggcga
    11641 agagccgctg gcaaaatgga taacccgccg tgccgacgac aaaaacgact acattttcaa
    11701 cgccagcccc atcagcagcg catcccacct tgccaacagc ctgtggcggc gtgcggcagg
    11761 cgcggtattg acttccgcca ccctgcaatc cttgggcaac ttcaacctga tgctgcgcca
    11821 aaccgggctg caatggctgc ccgaaaccac caccctcgcc ctcaaaagcc cctttgactt
    11881 tgaaaaacag ggcgaactct acatcccccc catatacgcc agccccaaag accccgaagc
    11941 ccacaccgcc gccatcatcg aatggctgcc caagcttatt tcgcccaccg aagccatcgg
    12001 cacgctcgtc ctgttttcct cgcgcaaaca aatgcaggat gtcgccctgc gcctgcccgg
    12061 agactacctg ccgctcttgc tcgtacaagg cgaattaccc aaagccgtcc tcctgcaaaa
    12121 acaccaccgg gccatagaag aaggcaaagc cagcatcatc ttcggactcg acagctttgc
    12181 cgaaggactc gacctgcccg gcaccgcctg cgtgcaagtc atcatcgcca aacttccctt
    12241 cgccatgccc gacaacccca tcgaaaaaac ccaaaaccgc tggatagaac agcgcggcgg
    12301 caaccccttc atcgaaatca ccgtccccga agccggcatc aaactcatcc aggccgtcgg
    12361 ccgcctcatc cgcaccgaac aagactacgg ccgcgtaacc atcctcgaca accgcatcaa
    12421 aacacagcgg tacggccaac aattattagc cggcctgcca ccgtttaaaa ggatagggta
    12481 aacctaccgc ccttcccgca acagaaagga aaagtcatga acttcacccg actgctcaac
    12541 caagtcttaa gcacggttca aaaaaaaggc aacacattct ccgacagccc gctcaattca
    12601 ttcggcggag gcgcgctggt tgccggtgtc gcctccatgc tgctgaacgg taaaaaccgc
    12661 aaaaccatca ccaaaatcgg ttcgaccgcc gctttgggct acctcgccta ccggggctat
    12721 cagatgtggc agcaaaacaa agggcgggca accgtaacac aaagcgattt ccaacctgcc
    12781 ggagaaaccg aagaaacata cagccgcacc gtattgcgca ccatgatagc cgccgccgct
    12841 tcagacggca tgatagacga agccgaacgc cggactatcg aacaggaaag cggcacagac
    12901 cccgaaactg ctgcatggct cgccgccgaa taccgcctgc ccgcaagcat cgaagacatc
    12961 gccgccgccg tcggcaacga tgaggcgttg gcggcggagg cctatctggc ggcaaggttg
    13021 gtctgtgccg atttgtcgcg gaaagaaacc gtcttcctcg cccgcctgtc gcaggctttg
    13081 aaactggatg acaatctggt ggagagtttg gaaaggcaat tggggttttg atgacacagc
    13141 cggggaggga aaatcatcaa aaaacggtta ttccgacccc ggcctcaaaa cggc
//
LOCUS       DAWFJW010000055        13150 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFJW010000055 DAWFJW010000000
VERSION     DAWFJW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.055"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WGR19_001232"
     CDS             complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WGR19_001232"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0798765.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLTAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFDFELVVGDFAQADEGEYFGALLQYVGKDG
                     DVQDLQSVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIADRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYEDLVDLCRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFTDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIATLKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3122..3481)
                     /locus_tag="WGR19_001233"
     CDS             complement(3122..3481)
                     /locus_tag="WGR19_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798766.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYAIYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVLAGFMVLVSGFPLSLLLVVLLVLTCCILARCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3429..3659
                     /locus_tag="WGR19_001234"
     CDS             3429..3659
                     /locus_tag="WGR19_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0798767.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3652..4293)
                     /locus_tag="WGR19_001235"
     CDS             complement(3652..4293)
                     /locus_tag="WGR19_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0798768.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4283..4834)
                     /locus_tag="WGR19_001236"
     CDS             complement(4283..4834)
                     /locus_tag="WGR19_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0798769.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKQQAFQTAFEGFDINTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4838..5356)
                     /locus_tag="WGR19_001237"
     CDS             complement(4838..5356)
                     /locus_tag="WGR19_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0798770.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5356..6996)
                     /gene="pqiB"
                     /locus_tag="WGR19_001238"
     CDS             complement(5356..6996)
                     /gene="pqiB"
                     /locus_tag="WGR19_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0798771.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLSAGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFNKLPLDKTVSGLN
                     ASLAELKSTLKSINTLSNNPQTQNIPNELNQTLKELRQTLQGVSPQSPIYGDVQNTLQ
                     SLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(6989..8323)
                     /locus_tag="WGR19_001239"
     CDS             complement(6989..8323)
                     /locus_tag="WGR19_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0798772.1"
                     /translation="MPPLIFSTFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     ADVPQLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8622..9251
                     /locus_tag="WGR19_001240"
     CDS             8622..9251
                     /locus_tag="WGR19_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0798773.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            9426..11801
                     /gene="hmbR"
                     /locus_tag="WGR19_001241"
     CDS             9426..11801
                     /gene="hmbR"
                     /locus_tag="WGR19_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ0798774.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYPVEGAGKETNIRGSARGIPDPSKHKYHNFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLTASSWREADDVNRRRNANLFYEWMPDSNWLSSLKADFDYQKTKV
                     AAINYKGLFPTNYTTWETEYHKKEVGEIYNRSMDTRFKRFTLRLDSHPLQLGGGRHRL
                     SFKTFASRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSSR
                     AGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDITSG
                     YRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGMLDANLYQSNYRN
                     FLSEEQKLTTSGTPGCTEENAYYSICSDPYKEKLDWQMKNIDKARIRGIELTGRLNVD
                     KVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLTY
                     LGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAGV
                     YNLFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
     gene            complement(12009..12131)
                     /locus_tag="WGR19_001242"
                     /pseudo
     CDS             complement(12009..12131)
                     /locus_tag="WGR19_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            12524..12772
                     /locus_tag="WGR19_001243"
     CDS             12524..12772
                     /locus_tag="WGR19_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0798775.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            12769..>13150
                     /locus_tag="WGR19_001244"
     CDS             12769..>13150
                     /locus_tag="WGR19_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0798776.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNAS"
BASE COUNT         3202 a         3280 c         3582 g         3086 t
ORIGIN      
        1 tttgtccgtg tggaaatgac atcaaagggt gtgaataggc ttttcatctt tgaaatctgt
       61 ctgcccgatc ggatgaataa aaagtaggat gataaaaaag ctgatgtatg gatactggtt
      121 taacccattt gttttaaagt tatagtcgag atacgtgtat cggtgcaacc agtctgccgt
      181 cgacgaaaat gccgtctgaa agtgtttcag acggcatttg tctccggggt ttcaacggtc
      241 agtcttcata attttccatc ggtgggcagc tgcacacgag attgcggtcg ccgtacacgt
      301 cgtccacgcg gttgacgaag ggccaaaact tgtgttcgcg gacgaacggc aacgggaaga
      361 cggcttcttc gcgggaatac ggatgcgccc agttgccggt tatatctgcg gcggtgtgcg
      421 gagcattcac cagcggatta tcgtctttcg gccattcgcc gtcgatgact ttctgcactt
      481 cgcggcgaat ggatttcagg gtggcgatga agcggtcgag ttcggctttg ctctcgcttt
      541 cggtcggttc gatcatcagc gtaccggcaa cggggaagga aaccgtcggg gcgtggaagc
      601 cgtagtccat caggcgtttg gcgatgtcgg tttcagtgat gccgctttcg gctttgagcg
      661 gacgcaaatc gacgatacat tcatgcgcga cgcggccgtt tttgcctgta tacagaatcg
      721 gatagtcttc gctcaggcgt ttggcaacgt agttggcgtt gagcaatgcc cagcgtgtcg
      781 cctgttccat gccttgtttg cccatcatgg tcaggtacat ccaagtaatc ggcagtatgg
      841 acgcagaacc gaaggccgca gcagcaacgg cggtttggtc ggcactggcg ctgtgggtgt
      901 cggtcaaagc gtgtcccggt gcgaacggag ccagatgggc tttcaagcca atcggaccca
      961 tgcccggacc gccgccgccg tgagggatac agaaggtttt gtgcaggttc atgtgcaaca
     1021 cgtccgcgcc gacttcggca ggctgcatga tgccgatttg ggcgttgagg ttggcgccgt
     1081 ccatgtaaac ctgtccgccg ttttcatgga cgatgcggca gatgtcgcgg atgccttctt
     1141 cgtacacgcc gtgggtggac ggataggtaa tcatgatggc agacaaagcg tcgcggtgtt
     1201 gctcggcttt ggctttcaaa tcgtcaatgt tgacgttgcc gtgttcgtcg gtgtcgacga
     1261 cgacgacttt caaaccgagc atggcggcgg tggcggggtt ggtaccatgg gctgattttg
     1321 gaatcagaca gatgttgcgg tgtccctcgc cattggcttc ttgatagcgg cggatggaga
     1381 gcatgccgct gtattcgccc tgcgcgcctg agttgggctg gaaggaaatc gcgtcaaagc
     1441 cggtgatggc tttcaagctg ttttccatat cggcgagcaa ttcgcgatag ccggcggttt
     1501 gcgcttcggg agcgtaaggg tggatgtcgg tgaactcagc ccaagtaatc ggcaacattt
     1561 ccgcagtcgc gttgagtttc atggtacagc tgcccaatga aatcatgctg cggttcatcg
     1621 ccaagtcgcg gtcttcgagt tttttcaggt agcgcaacat ttcgtgttcg gtgtggtaac
     1681 tgttgaacac aggatgttgc agaatgtcgt cttgacgcag caattcggcg ttcagacggc
     1741 ctttgacatc atcggcaaat gtgaacgtat ctttgccagt aaacgcgcgg cacaaatcaa
     1801 ccaaatcttc gtatgccgac gtttcatgga atgcagccgc aacttgagta tcgttgacgc
     1861 ggcgcaggtt gtaacccgac tctaaagcag cggcaaacac ttggtctgct ttctctttgt
     1921 tgccgaaatc gacggtaacg gtatcgaaga agactttgtg aaccacattc aggccgtctg
     1981 aaaccagcgc atcggcaaag gcagaagcca gcgcgtgaat gcggtcggcg atgcgtttca
     2041 cgccttcagg gccgtggtaa acggcataca tacccgccaa attcgccagc aatgcctgcg
     2101 cggtacaaat attggatgtc gctttttcgc ggcggatgtg ttgctcgcgg gtggacaacg
     2161 ccatacgcaa ggcaggtttg cccgatgcgt ctttggatac accgatgata cggcccgggg
     2221 cggaacgttt gaacgcgtct ttaaacgcga aataagcagc atgcggaccg ccgaagccca
     2281 tcggcacgcc gaagcgttgc gtgttgccca acgcaatgtc tgcacccaat tcagccggag
     2341 acttcagcaa aaccaagctc atgatgtcgg cggaaacggc gacaatcgtg cctttggctt
     2401 tcagacggcc tatgacgctt tgcaagtctt gcacgtcgcc gtctttgccg acgtattgga
     2461 gcagcgcgcc gaagtattca ccttcatccg cttgggcaaa atcgccgacc accagttcaa
     2521 agtcgaaata tttggcgcgg gttttcatca cgtccaaagt ttgcggatac acgcgcgcat
     2581 ccacaaagaa acgctcggat ttcaccttgc ccacgcggtg cgccatcgcc atcgcttcag
     2641 ccgcagcagt tgcctcgtcc agcaaagacg cgcccgccac agggaaaccg gtcaaatcga
     2701 tacacacttg ttggaagttt aacaacgctt ccaaacgacc ctgggcaatt tccgcctgat
     2761 acggcgtgta ggcggtgtac cagcccggat tttccaacac gttgcgcaaa atcacgttcg
     2821 gcacgcgggt cgggtaatag cctaaaccga tataggactt gttgattacg tttttcgacg
     2881 caatgccttt caatttcgcc aatgcgtccg cctcggtcaa ggcctcgggc aaatcgagtt
     2941 cggacggcat acggatgctt tgcggcacgg tattgccgac aaaatcgtcc atgcttttct
     3001 cgccgacagc ggtaagcaac gccgcttcgt cgccgaaact caaatgccgc gcggcaaatt
     3061 cgtcggggtt gaacagttcg gacagtttca tttctactcc ttcgggtttg tagggctgcc
     3121 atcaggcaga atttttagac atcggatgat tcgggcaagg gtagcacaaa agacgggata
     3181 atggcggaca acgcgctaga atgcagcagg tcaggacaag cagcaccaca agcagcagcg
     3241 ataagggaaa gccgctgacc aaaaccatga aacctgcaag caccagccaa gccaatggat
     3301 acgccggaag cggaaaaaca atattgagca gaaccaaaat cacgacaaac agcggcagcc
     3361 ccgccatcag gttggcacgc ctgtatattg cgtacgggtc ggatgcttct tcaggttcgc
     3421 aatattccat gatttcagcc tgtccgggtt ttatccacag caggctcgga cggtcggtca
     3481 tgccgtccga accggcaggc cgtctttttc aaaaaacgcc ataccgctgc aacttcatgc
     3541 ggctgtatgg cgtaggtttt gcacttcaga cggcatatgc gtcccgaagc agtttggtgt
     3601 cccagtcggt tcatgattat ataaacaact tgggtgcgga taaacctttg tttatctaaa
     3661 cccctgtttt ctcaaaaatt gattcaaatc ttcggcaaat ttccgatagc ctttgccgtt
     3721 ggcgtggatt tggtcggatt tcagattatt atcgcccaaa atttccgccc acgcgccgcc
     3781 gaacagcgga atgccgtatt cctcggacaa atcctcatac agcggatggt cgctcaaatg
     3841 tccgaacaac gcgcccagtg tgatgtgcgg cacgccgacg aggacggcgg ggatgttttc
     3901 cttctgcacg gtttcgatga ttttcgcgat attggcgcgg gtctgctcct cgggaacttt
     3961 gcgcagaaag tcgttgccgc ctatgccgac aatcacaagc ttgggtttgc gtgccaacag
     4021 cgcgggcagg cgcgacaggg cttgggcgga tgtatcgccc gatacgccgc cgttgacaat
     4081 attccaaccc gtcagttttt gcagttgcgc ggggtaggat tcgccggggt ttgcgccgta
     4141 gccgaaggta agcgaatcgc ccaaggcaag tacggtgctt ccttcgggaa ttttggcgtg
     4201 ggttcgggcg gattttctgc cgcaggcggt aaggagcaac gcgcctgcgc cgaggaggaa
     4261 ggttcttctg ttcattggtt tttcagatgg catcgagcca tttcgggtgg tatttcaggg
     4321 tttcacggta aaccggacag ctttcggcgt gcgcgccttt gaggtagccg gtactcatca
     4381 gaaattcgcc gacgatttcg ccgccgacga atttgaaatg ttttttaaag agtttaaccc
     4441 attcgtcttt gcttcgcgga tggtgcgcgt cgagccagtt cttgaacgag ccgtattctt
     4501 gttgcaacgc ttggatttgc cgtgcattga aaatggcggc atcgattttc aggcggttgc
     4561 ggacgatgcc cgcgtcggca agcaggcgtt cgcggtcggt gtcgtcgaag gcggcaacgg
     4621 tattgatgtc gaacccttca aatgctgtct gaaacgcctg ctgcttcttc agcatcagcg
     4681 tccagtttaa tcctgcctga ttgatttcca acaccagccg ctcaaacaat tcattgtcgt
     4741 cctcaatcgg aaaaccgtat tgcgtgtcgt ggtaatgttt gttggggtta tcggtgtttt
     4801 cgggaagtga ggcggcaaat tcgcaatagt tcacggttta ctcgaccatc tgttgcgccg
     4861 cctgtttcag tccctgttcg agtgcggcag tcatggcggc gtagccgtca ccctgctgtt
     4921 cggtttcgat atggaagggt ctgttcgtac cgtcgggtag gacggcgtag ccgctgatga
     4981 gggttttgcc cgtgtagctg ccttggaatg cgtcgatata gaccgtccat ttttcggtac
     5041 tgccgctgcg tgaggcagga acaaagatgc gtgtgctgtc caaacggttg aatgcattgc
     5101 tcaacgccgc ttcgagcata tcgtccaagg tgtccgccca aacgtggttt tgtgcggtgt
     5161 tgaggcggta ggggtcggtt tgatagacca gtccgccgcg tttgagcggt tcggcaaggc
     5221 ggacttcgac ggcggtttcg ccgccttgcg ttgcagggcg gatgtagcgg ctgtcgggca
     5281 acacgaaata ttgtgtgctt tgcacagtac cgcaggcggc aagcgtcagg gcggcggcga
     5341 tggggaaaag gcgcattatc ggcttccttt cgggataggg tctttgctgc tgctgttgaa
     5401 aatcagcgcg ttgggttttt ctttcaaagt gttaatgacg ggttgaacgt cttttaaggt
     5461 tttgtccaaa ctttgcagcg tattttgcac gtcgccgtag ataggcgatt gcggcgatac
     5521 gccttgcagg gtttgacgca attctttcag ggtttggttc agctcgttcg gaatgttttg
     5581 cgtttgcgga ttattgctta gcgtgttaat ggatttcagc gtggatttga gttcggcgag
     5641 cgaagcgttt aaacctgaaa cggttttgtc taaaggcagt ttgttgaact tgtccagcaa
     5701 atccgccaat ttgacctgca agtcgtccaa accgccgccc cgtgtggcaa tgacggtatc
     5761 gcctgcataa acggtatgcg gtcgcagctt gggcgaggcg gaaggctgat cgttcagttc
     5821 aatcattttg ctgccggtca gcaggttgtt gctggagatg gtggcagtca ggcctttatt
     5881 taaggccgtc tgaaattgtt gtttccaatg ttctttgctt tgctcgtcgg cattgatttc
     5941 caaacgggaa ggctcgatgc ggatgcgtac gggaatccaa ccgttttcaa acaaatgcag
     6001 gctgtcgttg cggtcgaaat aagggacatc ggaaaccaca ccgacattca ggcctttgta
     6061 ttcgacaggt gaaccggcag acaggccgcg tacggattgt ttgaaaaacg cggtgtagta
     6121 cagcgagcgg tcgtcaggca ggttggcgac ttcgctgcgg ctgtcgtaaa gcgtgaagct
     6181 gtcttcgctt ttgacgtttt tactgttttt ggttttcggc gaatcaaatg aaatcgcgcc
     6241 cgacagcagg gcaggcagag gggcggaatt gagtttgatg ccgctgcctg tggtttcgat
     6301 attgatgccg ctttccagcc aaaaacggct ggcggaatga atcagtttgt cgttggggct
     6361 ttggatgaag atggtgtaat gcacgctttg gtcggacggg tcgaaatgcg cgctttcgac
     6421 ttgcccgacc ataaagtttt catacaaaac agggctgttg acgttgagga tgcggtcgtt
     6481 tttaccaatc aaattcaagc gcagcccgct ttgcccgatg gcggtaacgg gcggaatgtc
     6541 ctgcacttgg aacacgtctt ttgcctcgtc gcttttgccg ggtgtaaaag cgatgtacga
     6601 acccgaaagc agcgtaccca aaccggttac gccgctttgg tcgatacgcg gcttgaccac
     6661 ccaaaactgg gtatcgctgc ggatgaggcc ggatacgtcc gcattgagtt gggcggttac
     6721 ttccacgcct ttttggtcgt cgcgcagttt gattcgggta acgcgtccga catcgatgct
     6781 caatactttg atgaccgtat tgttgacctc aatgccttcc gcgctgtcca tcaagagcgt
     6841 aaccacaggc cccctgttgc ggatttcctt aacccaaagc cagccgccgg caatcagcgc
     6901 aatcagcggg accagccaga cggcggagag caaggtgttg tttttgcgga cgcgtgcttg
     6961 ggcgtgtccg tttggagggg ggctgttgtc agtcatattt ttccgtttcg ttgaaagcaa
     7021 tgccgtctga agcgcgtttg tcccaaagca ggcgcgggtc gaaataatag gcggacagca
     7081 tcgtcagaat cacgaccagg cagaaataga ctgccgcact gcccggaatg acgcgcgcgg
     7141 cataagtgtg gaacgaacac atcaaaataa taatcacaaa aatatcaatc atcgaccagc
     7201 ggccgaccgc ttcggtgatg cggtagaggt gcgacaattt ctttgcgccc gttggcaaag
     7261 cgaagcgggc ggacgcaatc aaaaccgaca ttgccgcaat cttcagtacc ggcaccaaaa
     7321 tactcgcgct gaaaataacc gccgcaatca gcctgtcgcc ctcgtcccac atataagcga
     7381 tgccgttaag gatggtattg acctccgtgg cggcaggatt ggacgaaatc ataatcggca
     7441 ggatattggc agggaaatac aaaataaccg ccgccgtcag aaacgccgac gaaatactca
     7501 gacttttcgg ccgccggcgg tacagttccg caccgcacac gccgcagggg gattcggcac
     7561 tgtcgcggaa atacaggcag cggctgcaac aggttttgcc ttctgatgcc gtctgaaccg
     7621 cattatcccc cgtcagccgc ccgatttgaa aatacaccca atgctgggga acggataccg
     7681 aagtccgaat cagcataacc gacagcgcga acatcagata aaacgccgac ccgaagcgaa
     7741 cctctgccac agacgagagc ttgatatacg ccaccagagt ggaaacaaaa aacacatcca
     7801 ccatcatcgc ctgcctcaag cgcaccatta cgcgtgttgc caaacgcagc gcaggatacg
     7861 cctgtttccg tatcagcgcg gcatagacat acaggcacag cagcagaaac agaaccggcg
     7921 cgccgaaggt cagcacaaac atcacttcgg ctaaaaaacc ataatcctga aacaccatca
     7981 ggcgcatcat ctcgggcagc gaaaggacgg atgccgcacc cggtatcccg acctcgatat
     8041 acgtcatacc gtaagcaaac gccatcaaaa tcagcgaagc cgccgcataa gcgggcgggg
     8101 cggaaaaagg atgcctgccc accctgaaga gcttatagcc gcagcgcggg cagaacgccg
     8161 cctcccccct gtccaattgc ggcacatccg cgcggcagcc gcattccggg caatctaccg
     8221 tatgcggcgg cagggcttcg tcgcggcagt tgccgcgaag gctgtggggg atattttcgg
     8281 caaacggctt catcgggaaa gtggaaaata taagtggcgg cattataaag gaaaaaagca
     8341 acgcacccaa tcaggatgca aaaccggtag gttcggcagt atgaacaaac ccttgtcgtc
     8401 cggcaaaaat attttttgag cggctgtccg ccgactgccg aacccatttg ataaaatccc
     8461 gattctgtaa agaatagtaa aatacctgct taaaaacatc acgcaaatga tattttttcc
     8521 aaaaatatgt gataataaat ctcgtttatt atttttgtat gcggtcaggt acggattttg
     8581 cccgcactgc atccttaccc cttaccgaca aaggaaaagc tatgagcgaa accgaaaatc
     8641 aagccctgac gtttgccaaa cgcctgaagg cggatactac ggcggttcac gacagcgtgg
     8701 ataacctcgt tatgtctgtc caaccgtttg tcagcaaaga aaactacatc aaatttttga
     8761 aactccaatc cgttttccac aaggctgtcg accacatcta taaagatgcc gaattaaaca
     8821 aagccattcc cgagctggaa tacatggcgc gatacgatgc cgtaacgcaa gacttgaaag
     8881 acttgggcga agagccttac aaattcgaca aagaattgcc acacgaaacc ggcaacaaag
     8941 caatcggctg gctttattgc gccgaaggtt ccaatttggg cgcggcgttt ttgttcaaac
     9001 acgcgcaaaa acttgattac aacggcgaac acggcgcgcg ccacctcgct ccgcatccgg
     9061 acgggcgcgg caaacactgg cgcgctttcg tcgagcatct gaacgccttg aacttgactc
     9121 ctgaagccga agcggaagcc atccaaggcg cgcgcgaagc ctttgcattc tacaaagtcg
     9181 tgttgcgcga aaccttcggc ttggcagccg atgccgaagc ccccgaaggt atgatgccgc
     9241 acaggcacta aaaaataatc gaaccaaata aacaaggtct cggcatagct gtttgcaggg
     9301 acctttaatt acacggcgcg gctttgttta catggattac tgtcttatta aatattaatg
     9361 attatcataa aatctattat tcgctaaccg atggatgaac aatccataca tcttgagttg
     9421 ataatatgaa accattacaa atgctcccta tcgccgcgct ggtcggcagt attttcggca
     9481 atccggtctt tgcggcagat gaagctgcaa ctgaaaccac acccgttaag gcagaggtaa
     9541 aagcagtgcg cgttaaaggc cagcgcaatg cgcctgcggc tgtggaacgc gtcaacctta
     9601 accgtatcaa acaagaaatg atacgcgaca acaaagactt ggtgcgctat tccaccgatg
     9661 tcggcttgag cgacagcggc cgccatcaaa aaggctttgc tgttcgcggc gtggaaggca
     9721 accgtgtcgg cgtgagcata gacggtgtaa acctgcctga ttccgaagaa aactcgctgt
     9781 acgcccgtta tggcaacttc aacagctcgc gtttgtctat cgaccccgaa ctcgtacgca
     9841 atattgaaat cgtgaagggc gcagactctt tcaataccgg cagtggtgca ttgggcggcg
     9901 gtgtgaatta ccaaacgctg caaggccgtg atttgctgtt ggacgacagg caattcggcg
     9961 tgatgatgaa aaacggttac agcacgcgta accgtgaatg gacaaatacc ctcggtttcg
    10021 gtgtgagtaa cgaccgcgtg gatgctgctt tgctgtattc gcaacggcgc ggccatgaaa
    10081 ccgaaagcgc gggcaaccgc ggctatccgg tagaaggtgc gggtaaagaa acgaatatcc
    10141 gcggttccgc ccgcggcatc cccgatccgt ccaaacacaa ataccacaac ttcttgggta
    10201 agattgctta tcaaatcaac gacaaccacc gcatcggcgc atcgctcaac ggtcagcagg
    10261 ggcataatta cacggttgaa gagtcttata acctgaccgc ttcttcctgg cgcgaagccg
    10321 atgacgtaaa cagacggcgc aatgccaacc tcttttacga atggatgcct gattcaaatt
    10381 ggttgtcgtc tttgaaggcg gacttcgatt atcagaaaac caaagtggcg gcgattaact
    10441 acaaaggcct gttcccgacg aattacacca catgggaaac tgagtaccat aaaaaggaag
    10501 ttggcgaaat atacaaccgc agcatggaca cccgattcaa acgttttact ttgcgtttgg
    10561 acagccatcc gttgcaactc gggggggggc gacaccgcct gtcgtttaaa actttcgcca
    10621 gccgccgtga ttttgaaaac ctaaaccgcg acgattatta cttcagcggc cgtgttgttc
    10681 gaaccaccag cagtatccag catccggtga aaaccaccaa ctacggtttc tcactgtctg
    10741 accaaattca atggaacgac gtgttcagta gccgcgcagg tatccgttac gaccacacca
    10801 aaatgacgcc tcaggaattg aatgccgagt gtcatgcttg tgacaaaaca ccacctgcag
    10861 ccaacactta taaaggctgg agcggttttg tcggcttggc ggcgcaactg aatcaggctt
    10921 ggcgtgtcgg ttacgacatt acttccggct accgtgtccc caatgcgtcc gaagtgtatt
    10981 tcacttacaa ccacggttcg ggtaattggc tgcccaatcc caacctgaaa gccgagcgca
    11041 gcaccaccca caccctgtct ctgcaaggcc gcagcgaaaa aggcatgctg gatgccaacc
    11101 tgtatcaaag caattaccgc aatttcctgt ctgaagagca gaagctgacc accagcggca
    11161 ctcccggctg tactgaggaa aatgcttact acagtatatg cagcgacccc tacaaagaaa
    11221 aactggattg gcagatgaaa aatatcgaca aggccagaat ccgcggtatc gagctgacag
    11281 gccgtctgaa tgtggacaaa gtagcgtctt ttgttcctga gggctggaaa ctgttcggct
    11341 cgctgggtta tgcgaaaagc aaactgtcgg gcgacaacag cctgctgtcc acacagccgc
    11401 tgaaagtgat tgccggtatc gactatgaaa gtccgagcga aaaatggggc gtattctccc
    11461 gcctgaccta tctaggcgcg aaaaaggtca aagacgcgca atacaccgtt tatgaaaaca
    11521 agggctgggg tacgcctttg cagaaaaagg taaaagatta cccgtggctg aacaagtcgg
    11581 cttatgtgtt tgatatgtac ggcttctaca aaccggctaa aaacctgact ttgcgtgcag
    11641 gcgtgtacaa cctgttcaac cgcaaataca ccacttggga ttccctgcgc ggtttatata
    11701 gctacagcac caccaatgcg gtcgaccgcg atggcaaagg cttagaccgc taccgcgccc
    11761 caggccgcaa ttacgccgta tcgctggaat ggaagtttta atctggtatt attgaattaa
    11821 tcgccttgtt gaaaattaaa gccgtccgaa ttgtgttcaa gaattcattc ggacggcttt
    11881 ttaccgtaaa tctgtgtgat ggggttttat attgatacag tatccgaact caggcagggg
    11941 gtaatgttgc aggattaaaa atgccgtctg aaaaatgttt cagacggcat tttttggttg
    12001 ccgatatgtt aacggacgac gttggcgcgc atgaccacgc ggacgtggaa cgagcctgcc
    12061 tgacagtcgt attgtccgcc ttgcagcggc tgcttgtcga agtagctgtg gaagccggta
    12121 acgccgcgtc cgcaagggtg gcggcagatg ccgcattgtg tttataatgc cgtgtcagac
    12181 gtttgattta tgggaaaata atgatgctta ccaatcagga agtaaccggt gtcaaactgg
    12241 gataccgcaa agccgtttta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
    12301 agacagtaca gatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt
    12361 ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact ataaatagca
    12421 aggtgtgaat aaaacggcgg atataatcaa atatatgtaa tgagattccc aaaatcttgg
    12481 taatgatgta tattagtcaa gttagctaat catgagaagt gttatgtttc aagcaaatat
    12541 tcatcaggca aaaaccaatt tgagtcaatt gattcaaaga gcagaagcag gggaaatcgt
    12601 tattattgcg aaggcaggta agccttgcgt ccaactaatc ggtattgaaa aaccggcacg
    12661 aaatgcagga aggttgaaaa aattcagtca tatggaaaat acggatattt cacgtattct
    12721 tgaggatgac aatgaaacgg cagctttatt ttttgaggag tcggctctgt gagaaagatt
    12781 ctgcttgata cccatgcgtt gctgtggtgg ttgttggatg acaagaaact ggggatatct
    12841 gcacgcaaac tgatagaaaa tccgagaaat gcgatctttg tcagtgcagc aagtatttgg
    12901 gaaatttcca tcaagcagaa caaggggttg ttgaaattac cggaagagtt ttttgatgtg
    12961 cttcaagaag aggattttga aatgttgcct ataggtctgt ttcatgcaaa acaggctgga
    13021 agccttccgg agatacacaa agaccctttc gataggatgt tgattgcaca aacccaagcg
    13081 gaaggctttg aactgatgac tgtcgatgaa tatattccgc aatatggaat tagagttgtc
    13141 aacgcatcaa
//
LOCUS       DAWFJW010000056        13139 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFJW010000056 DAWFJW010000000
VERSION     DAWFJW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.056"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..723
                     /locus_tag="WGR19_001245"
     CDS             <1..723
                     /locus_tag="WGR19_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0798777.1"
                     /translation="LSIPDYSTLCRYRNRLAQDNTLSELLELINRQLTEKNLKIEKAS
                     AAVIDATIIQTAGSKQRQAIEVDEEGQVSGQTTPSKDSDARWTKKNGLYRLGYKQHTR
                     TDAEGYIEKLHITPANTHECNHLLPLLEGITEGTTIYADKGYDSAENRQHLEEHQLLD
                     GIMRKACRNRPLSEVQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQS
                     HLKAMCLNLLKAANRLSAPVAA"
     gene            complement(1044..1973)
                     /locus_tag="WGR19_001246"
     CDS             complement(1044..1973)
                     /locus_tag="WGR19_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0798778.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            2104..2853
                     /locus_tag="WGR19_001247"
     CDS             2104..2853
                     /locus_tag="WGR19_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0798779.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2976..3557
                     /gene="petA"
                     /locus_tag="WGR19_001248"
     CDS             2976..3557
                     /gene="petA"
                     /locus_tag="WGR19_001248"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0798780.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            3576..4925
                     /locus_tag="WGR19_001249"
     CDS             3576..4925
                     /locus_tag="WGR19_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0798781.1"
                     /translation="MANQTNSKAKALLGWVDARFPLSKMWKEHLSEYYAPKNFNFWYF
                     FGSLSMLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKLKDENGVPLDGIPFFPYYVVHDILAVTIFLIVFCAVMFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFAGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDTNKPVPERVTMSTTKQKIMFFVYVGITVVGAYLFATNI"
     gene            4940..5725
                     /locus_tag="WGR19_001250"
     CDS             4940..5725
                     /locus_tag="WGR19_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0798782.1"
                     /translation="MKQTLKNWFAALLLAVPMSAAVASGGGHYEKVDIDLRDQVSLQH
                     GAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDAA
                     KWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQG
                     VQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVNY
                     LVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLNKEFSKDIH"
     gene            6099..7883
                     /gene="rmuC"
                     /locus_tag="WGR19_001251"
     CDS             6099..7883
                     /gene="rmuC"
                     /locus_tag="WGR19_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0798783.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAVLNAQLVEKAA
                     RCDFIEQAHAEMASELAVLDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQEQKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYAAEDEAV"
     gene            complement(8090..8722)
                     /locus_tag="WGR19_001252"
     CDS             complement(8090..8722)
                     /locus_tag="WGR19_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0798784.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            8814..9638
                     /locus_tag="WGR19_001253"
     CDS             8814..9638
                     /locus_tag="WGR19_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0798785.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQEIAGWAMS
                     EKLDGVRAYWDGKHLVSRQGYVFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRYGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            9707..10270
                     /locus_tag="WGR19_001254"
     CDS             9707..10270
                     /locus_tag="WGR19_001254"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0798786.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            10351..10788
                     /locus_tag="WGR19_001255"
     CDS             10351..10788
                     /locus_tag="WGR19_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0798787.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            10857..11126
                     /locus_tag="WGR19_001256"
     CDS             10857..11126
                     /locus_tag="WGR19_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0798788.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            11126..12901
                     /gene="ptsP"
                     /locus_tag="WGR19_001257"
     CDS             11126..12901
                     /gene="ptsP"
                     /locus_tag="WGR19_001257"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0798789.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYNAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
BASE COUNT         3288 a         3264 c         3642 g         2945 t
ORIGIN      
        1 ttgagcatcc ccgattacag caccttatgc cgctaccgca accggctggc gcaagacaac
       61 accctgtccg aattgctgga actgattaac cgccaactga ccgaaaaaaa cctaaaaata
      121 gagaaagcat ccgccgccgt cattgacgcc accattattc agaccgccgg cagcaaacag
      181 cgtcaggcca tagaagtcga tgaagaagga caagtcagcg gccaaaccac accgagtaaa
      241 gacagcgatg cccgttggac aaagaaaaac ggcctctaca gactcggtta caaacaacat
      301 acccgtaccg atgcggaagg ctatatcgag aaactgcaca tcaccccagc caatacccat
      361 gagtgcaacc acctgttgcc tttgctggaa ggcattaccg aaggcacgac catctatgcc
      421 gacaaaggct atgacagtgc ggaaaaccgg caacatctgg aagaacatca gttgttggac
      481 ggcattatgc gcaaagcctg ccgcaaccgc ccgctgtcgg aagtgcaaac caaacgcaac
      541 cgatatttgt cgaaaacccg ttatgtggtc gaacaaagct tcggtacgct gcaccgtaaa
      601 ttccgctatg cccgggcagc ctatttcgga ctgattaaag tgagtgcgca aagccacctg
      661 aaggcgatgt gtttgaacct gttgaaagcg gccaacaggc taagtgcgcc cgttgccgcc
      721 taaaaggcag cccggatgcc tgattatcgg gtatccgggg aggattaagg gggtattggg
      781 gtaaaattag gaggtatttg gtacgaaaac agccgaaatc ctgtgtttgg gtttcggctg
      841 tcgggaggga aaggaatttt gcaaaggtct cagattgggc aaaaatcaaa aacagtatag
      901 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa
      961 ggcgaggcaa cgctgtactg gtttttgtta atccactata catgaaaaaa tagaaaaact
     1021 gccgggtatg gttgaataag gggtcagacc ggttccaatt cgctcagtcc gggcaaatct
     1081 gcaaaaccgc gttcgcgtat gatttggcaa aaattgttca gatagctctt gtccgtatct
     1141 tcggtacgga tggcggcata cagtttgctt tgcaatccgt cggcagtaat ttggcggtgg
     1201 acgacatagc ctttttcaag gtagggcatg actgtccaat agggaagggc ggcaatgcca
     1261 cgtctgctgg caaccagttg gataatggcg atggtcagtt cgctgtgtcg gcgcggcggg
     1321 ttgatgtttt tcggaatcag gatttttttg ggcaaatcca gcatctcgtc gggaacggga
     1381 taagtaatca gggtttcccc gataaagtct tccgccgtcc aaacgttttt ggcggcaagc
     1441 ggatggtctg gtgcgcaaat gccgaccatt tcgtaggcaa acagcggttg gaaagaaata
     1501 tcgttttgtt tttccgcttc ggaaacaatg gcaaggtcgg cacggtgttg cagcagcagt
     1561 ccgacggggt ccgcttggaa tcccgatacg atatccaatt cgatttgggg ccatatcggg
     1621 cggaattcgc ccattgcggg catgagccag tcgaaacagg tatggcattc gacggcaatc
     1681 cgcaattctc ctgcctctcc ttctataatg cgtgccaaat cacgttctgc atcggtaact
     1741 tgaggcaaca ggtcgtgagc gaggcgtaac agcctttctc caaccggggt aaagcgcaag
     1801 ggcgtggatt ttcgttcgaa caacggtgcg ccgtagtggt tttcaagcat acggatttgg
     1861 tgggaaagtg cagattgggt aaggaaaacc cgtttggcgg caagggagac gctgccggtt
     1921 tcttcaagtg ccagcagggt cttgaggtgg cgcagttcga tgatggaatc catgaggttt
     1981 cagacggcat attgaaccgg cgcatattaa aataattcat atgcgggtgc aaatcggaaa
     2041 aatgtgaatt ccggacattt tgcgattaga atgcccgctt gtttaaagcg attaggaaaa
     2101 aagatggtat tgcgcaggga ttttctgact tggtgtaatg aaacattgca gacagcgttg
     2161 tttaaagatt acgcccctaa cggtttgcag gttgaaggga gggaatatat cgggaaaatc
     2221 gttacgtcgg taacggcaag cagggcagcg attgattttg ctgtggagca gaaggcagat
     2281 ttgcttttgg tacatcacgg tatgttctgg aaaaacgagt tgccgaccgt tactggttgg
     2341 aaaaaagaac ggattgccgc actgttacgg cacgacatca atatggcagg ctaccatctg
     2401 cccctggatg cacatcccac actgggcaac aatgcccaac tcgccgacag attgggtttt
     2461 gcgacagaaa aacggttcgg cgaacaaaac ctgctcaact cgggcagcct gaaacaagcc
     2521 aagacactcg gcgcattggc ggcgcatatt gaaacagttt tgcaacgtaa gcctgtcgtc
     2581 atcggcaaac ccgaacgcga aatccgacgg gttgcatggt gcagcggcgg ggcgcagggg
     2641 ttttttcaga cggcaataga cgaaggtgtc gatctgtatt tgacggggga aatttccgaa
     2701 gcccaatacc accttgccaa tgaaacgggt acggctttca tttcggcagg gcatcacgcg
     2761 acggaacgtt acggcgtacg cgcgctggca gaatcggcgg cagaggtttt cgggttggaa
     2821 gtgtgccatt ttgacgaaaa caatccggct tgagtcttga gaaatatcat aaaactttac
     2881 cttattttaa ataatgcttt gagtatcact gcaaactggg taaaattgca acgtttaatg
     2941 gctcggtatt cccaaaatat taggacgact gaataatgga taatcaagaa atcaacaacg
     3001 gccgccgccg tttcctgaca ctcgcgacct gcggcgcggg cggagtggca gcattgggtg
     3061 tggcaacgcc gtttgtggcc agttttttcc cttcggaaaa agccaaggcc gccggtgctg
     3121 ccgtcgaggt ggatgtcagt aaaatcgaag cgggtcagct gctgaccgcc gagtggcaag
     3181 gcaaaccgat ttgggtgctc aaccgtacag atcagcagct taaagacctg aaaggcctga
     3241 acggcgaact taccgatccc aattccgatg cggaacagca gccggagtat gcgaaaaacg
     3301 agacccgttc gattaagccg aacatccttg tcgccatcgg tatctgcacc catttgggct
     3361 gctcgcccac tttccgtccc gacattgccc ccgccgattt gggtgcagac tggaaaggcg
     3421 gcttcttctg cccctgccac ggttcgaaat tcgacttggc cggccgcgta tataaaggtg
     3481 ttcctgcccc gaccaacctg gttgtcccgc catataaata cttgagcgac acaactatct
     3541 tggtgggcga agactaagaa taaggaacga taattatggc aaaccaaacc aatagcaaag
     3601 caaaagcatt gttaggctgg gtagatgccc gttttccatt aagtaaaatg tggaaagagc
     3661 atctgtctga atactatgcg cctaaaaact tcaacttctg gtatttcttc ggctcattgt
     3721 ctatgctggt gctggtgatt caaatcgtca gcggtatttt cctgaccatg aactacaaac
     3781 cggacggcaa ccttaacgcc taccatctgc ctgctgcctt taccgcagta gagtacatca
     3841 tgcgcgacgt gtccggcggc tggattatcc gctatatgca ctctaccggc gcatctttct
     3901 tcttcatcgt cgtttatctg cacatgttcc gtggtctgat ttacggttcg tacaaaaaac
     3961 cgcgcgaatt ggtgtggatt ttcggttctt tgattttctt ggcattgatg gcggaagcct
     4021 ttatgggcta cctgttgcct tggggtcaaa tgtccttctg gggtgcgcag gtaattatta
     4081 acctgttctc cgccatcccg gttatcggtc ctgatttgtc cacttggatc cgcggcgact
     4141 tcaacgtttc cgacgttacc ctgaaccgct tctttgccct gcacgttatc gcagtacctt
     4201 tggtattgct cggcttggtt gtggctcata tcattgcctt gcatgaagtg ggttccaaca
     4261 accctgacgg tgtagaaatc aaaaagctga aagatgaaaa cggtgtccct ctagatggca
     4321 tacctttttt tccgtattat gttgtgcatg atatattggc agtaacgata ttcttgattg
     4381 tcttctgtgc cgtgatgttc tttgcacctg aaggcggcgg ctacttcttg gaagcgccaa
     4441 acttcgatgc agcgaatgcg ctgaaaacac ctccgcacat tgcgccggta tggtacttca
     4501 ctccgttcta cgcaattctg cgtgcgattc cttcctttgc cggtactcag gtatggggtg
     4561 taatcggtat gggcgcggcg gtcgtgttga ttgccctgct gccttggctg gataggggcg
     4621 aggtgaaatc cgtccgttac cgcggcccga ttttcaaaac cgcattggtc ttgttcatca
     4681 tttcctttat cggtttgggt attttgggtg caatggtagc aactgatact cgtactttgg
     4741 ttgcacgtat cctgtctttc gtctactttg cattcttcct gggtatgccg ttctatacca
     4801 aactggatac caacaaacca gttcctgaac gcgtaaccat gagcactact aaacaaaaaa
     4861 ttatgttctt tgtttacgtc ggtattaccg ttgttggtgc ttacttgttt gcaaccaata
     4921 tctgatgagg gcagcgaaaa tgaaacaaac tctgaaaaac tggtttgctg ccttattgct
     4981 ggcagtgcct atgagtgcag ccgtcgccag cggcggcgga cactacgaaa aagtcgatat
     5041 cgacctgcgt gaccaagtca gcctgcagca cggtgcgcaa atctttacaa actactgttt
     5101 gtcttgccac tctgcaagcg gtatgcgctt caaccgtctg aaagacatcg gtttgactga
     5161 cgaagaaatc aagaaaaacc tgatgtttac caccgataat gtcggcgatg tcatgcattc
     5221 ggcgatgaac ccgaaagatg cggcaaaatg gtttggtgct gctccgcccg atttgacgtt
     5281 gattgcgcgt tccaaaggtg cagactacct ttacgcctat atgcgcggct tctataaaga
     5341 tccgacccgt ccgagcggct ggaacaatac tgtattcgat aaagtcggta tgccccaccc
     5401 gttgtgggag caacaaggtg ttcaagccgt tgagttggat gccaaaggtc agccggttat
     5461 ggtaaaagac gaacacggcg agatgaagcc taagctgtat tgggaatcta ccggtttgca
     5521 cagccgccgc ctgcctaacg gcaaagtgat ccaaaaagag tacgacgcat atgtacgcga
     5581 tttggtcaat taccttgtgt acatgggcga acctgcacaa ctgcaacgca aacgtatagg
     5641 ctatgtcgtg atgattttcc tatttgcggt tatgctgcct ttggcttact tcctaaataa
     5701 agaattttct aaagatattc attaatcatt aaaaaagaat attggaaaga cgtacactaa
     5761 gcgtttggaa caaaagggca aatcctttag ggtttgccct tttttcattt tgcctgccgt
     5821 ttgaaaagcc tgaatccgta tgccgtctga acatagctgc aacatttcag acggcatcat
     5881 tctaaaaatg tcagacatcg ggaacttaat cagggtttgt ggcagattga tattgaatta
     5941 ggaaaaggtc tgaaaccgca agcagcatgg taaggcgatg tcgaacaggt ttaaagccaa
     6001 tccgctatat tttttggagc tatgtcgaac aaacaggtag aatcggaata agcggcggtt
     6061 ttgtccggtg tgaaaatgtt tgaatattag gatgattgat ggaactgatg actgttttgc
     6121 tgcctttggc ggcgttggtg tcgggcgtgt tgtttacatg gttgctgatg aagggtcggt
     6181 ttcagggcga gtttgccgta ttgaacgcgc aattggtgga aaaggcggca agatgtgatt
     6241 ttattgaaca ggcacacgcg gaaatggcat cggaattggc cgttttggat gggaaatacg
     6301 agcatttgca agacgaaaat tatgctttgg gcaaccgttt ttccgcagcc gaaaagcaga
     6361 ttgcccattt gcaggaaaaa gaggcggagt cggcgcgtct gaagcagtcg tatatcgagt
     6421 tgcaggaaaa ggcgcagggt ttggcggttg aaaacgaacg tttggcaacc cagctcggac
     6481 aggagcagaa ggcgtttgcc gaccaatatg ccttggaacg ccaaatccgc caaagaatcg
     6541 aaaccgattt ggaagaaagc cgccaaactg tccgcgacgt gcaaaacgac ctttccgatg
     6601 tcggcaaccg ttttgccgca gccgaaaaac agattgccca tttgcaggaa aaagaggcgg
     6661 aagcggagcg tttgaggcag tcgcataccg agttgcagga aaaggcacag ggtttggcgg
     6721 ttgaaaacga acgtttggca acgcaaatcg aacaggaacg ccttgcttct gaagagaagc
     6781 tgtccttgct gggcgaggcg cgcaaaagtt tgagcgatca gtttcaaaat cttgccaaca
     6841 cgattttgga agaaaaaagc cgccgtttta ccgagcagaa ccgcgagcag ctccatcagg
     6901 ttttgaaccc gctaaacgaa cgcatccaag gtttcggcga gttggtcaag caaacctatg
     6961 ataaagaatc gcgcgagcgg ctgacgttgg aaaacgaatt gaaacggctt caaggattga
     7021 atgcgcagct gcacagcgag gcaaaggccc tgaccaacgc gctgaccggt acgcagaata
     7081 aggttcaggg caattggggc gagatgattc tggaaacggt tttggaaaat tccggccttc
     7141 agaaagggcg ggaatatgtg gttcaggcag catccgtccg aaaagaggaa gacggcggca
     7201 cgcgccgcct ccagcccgac gttttggtca acctgcccga caacaagcag attgtgattg
     7261 actccaaggt ctcgctgaca gcttatgtgc gctacacgca ggcggcggat gcggatacgg
     7321 cggcacgcga gttggcggcg catgtcgcca gcatacgcgc gcatatgaaa ggcttgtcgc
     7381 tgaaggatta caccgatttg gaaggtgtga acacattgga tttcgtcttt atgtttatcc
     7441 ctgtcgaacc ggcctacctg ttggcgttgc agaatgacgc gggcttgttc caagagtgtt
     7501 tcgacaaacg gattatgctg gtcggcccca gtacgctgct ggcgactttg cggacggtgg
     7561 cgaatatttg gcgcaacgag cagcaaaacc aaaacgcgct ggcgattgcg gacgaaggcg
     7621 gcaagctgta cgacaaattt gtcggctttg tacagacgct cgaaagcgtc ggcaaaggca
     7681 tcgatcaggc gcaaaacagc tttcagacgg catttaagca acttgccgag gggcgcggga
     7741 atctggtcgg ccgcgccgag aagctgcgtc tgttgggcgt gaaggcaaac aagcagcttg
     7801 cacgcgattt gaccgaacgt gccaatgaaa caacggcgtt gtcggaatct ttggaatacg
     7861 cggcagaaga tgaagcagtc tgacttgtgc ggaaaaatat tgtttcagcc ggggcgggaa
     7921 tgccgaaagc gcggcgtaac tgtactggtt ctgattttgg gtttgtttga agtacttacc
     7981 aaagccttgt ccggcgaggt acaccggcaa ggcggcggtt gtctgagcct tttgtgtttc
     8041 agacggcatc agtgcagatg atgccgtctg aagaccgtag ggaaggcggt tagaaaaacg
     8101 gattgtgccg cttttcgtgt ccgatggaag tcatacgccc gtgtccggcg acaacttgca
     8161 cggtttcggg aagggtgaat aatttgttgc ggatattatt gattaagtcg gcgtggttgc
     8221 cgcgcggaaa atcggttctg cctatggttt cgtaaaacag cacgtcgccc gcaatcagca
     8281 attccgcctc ggcacaataa aagacgatat gtcccggcgt atggcccgga atatgcagca
     8341 cttgaaaggc atagcgtccg accgtgagcg tttcgccttc ttcgagccaa cggttcggcg
     8401 caaaggcggg cgagacggga aatccgtatt gcgcggtggt ttgcggcagc gattggagca
     8461 ggaattcatc gtccggatgc ggcccgagga cagggacttt atgcgttttc aacatttcga
     8521 ccacgccgcc cgcgtgatcg agatggccgt gcgtcagcca gattgccgtg agcgtaagtt
     8581 tgcggtttgc caacgcttgc agcaggaacg gcacgtcgcc gccgacatcg gtcaggacgg
     8641 cttcgccgct ttcgtcgtcc caaatcaggg tgcagttttg gcggaagggg gtaacgggga
     8701 agatttcgta acgtagggtc atcggcgtgt tcctaaacgg tttttcagac ggcatcgggt
     8761 ttgccgtttg ttttatggcg gtttgccgcc cgttttgata ttgggggaat cagatgatta
     8821 agaagacaat cggcggcatc ataccgattt ttacggcggt tttcatccct gcatcagcag
     8881 gcgcggcgga tttgatgctg gcgcaggaat acaaaggtca agagattgca ggctgggcga
     8941 tgagcgagaa actcgacggc gtgcgcgcgt attgggacgg taaacattta gtcagccggc
     9001 aaggatatgt atttacaccg cccaaaggct ttaccgcgca gtttccgcct tatcctttgg
     9061 acggcgaatt gtatagcgga cgcggtcagt tcgagcagat ttccgctacc gtgcgttctg
     9121 cttcttcaga ctggcgcggt atccgcctgc acgttttcga tgtacccaag gcgcagggca
     9181 atctctacca acgtttggca gtcgcaacgc agtggctgaa aacgcatccg aacgcgccga
     9241 ttaccatcat cccacaaatc aaagtgcgcg accggcagca cgcgatggac tttttaaaac
     9301 aaatcgaagc gcagggcggc gaaggcgtga tgctgcgtca gcccgaatcc cgttacagcg
     9361 gcggcaggag cagccaatta ttgaagctga aaagccaata cgacgacgaa tgcacggtaa
     9421 cgcggcacta tgagggcaaa gggcgaaacg ccggacggtt gggcgcggtc ggctgcaaaa
     9481 accggtacgg cgaattccgc attggcagcg gcttcaaaga taaagaccga gacaacccgc
     9541 ccaaaatcgg cacactgatt acctaccgct accgtggctt tacgcagaaa ggcacgccga
     9601 aattcgccac gtttgtgcgc gtgcgtaccg accgctgagc aggacagttc agacggcatc
     9661 cgatactttg gtttataatt ttccttttac cgaccgattc cgacatatga ccgatttaga
     9721 aaccaaacgc cttgaaacac aggcgatgct tgaaaacgcc gatcttttgt tcgaccaagg
     9781 ccaatgccgt gccgcactgc aaaaagtggc ggacgagatt acgcgcgatt tgggcggcaa
     9841 atatccgctg ctgctgcccg tgatgggcgg cgcggtggtg tttacggggc agttgctgcc
     9901 gctgttgcgt tttcccttag attttgatta tgttcacgtt tcccgttacg gcgacaagct
     9961 ggagggcggc gcgttcaact ggaagcgtat gcccgatgcg gaacaaatcc ggggcaggca
    10021 cgtcgtcgtg ctggacgata ttttggacga agggcatacg atgtccgcca ttcaagccaa
    10081 acttttggaa atgggtgcgg caagctgccg tgcggcggtg ttcgccaaca agctgattga
    10141 caaacccaag cctatccgag ccgattatgt cggactggat gtgccgaacc gttatgtttt
    10201 cggttacggc atggatgcgg cgggctgctg gcgcaatctg ggcgagattt acgcattgga
    10261 cggaaaataa ggacgcgatg ccgtctgaag gcctttcaga cggcatcgcg cccatacgcc
    10321 ggtaggacaa cgaggaacag gacgcataat atgatagggc ttttaatcat cacacacgaa
    10381 accataggcg aagcctaccg caagctggca catcattttt ttccgggcgg actgcctgaa
    10441 aacgtccgca tactcggcgt gcaaccgacg gaagaccaag acgacatcat caacaacgcc
    10501 attgccgcgc ttcaggagtt tcccgacaac gacggcgtgc taattatgac cgatattttc
    10561 ggcgcgaccc cctgcaatgc agcccgccgc ctcgtgcgcg aaaacaaatc ggcgattttg
    10621 accgggctga acgcgccgat gatggttaag gccgtccaat attcgccggc ggcggaagac
    10681 cttgccgcct ttaccgaatg cgtcagggag gcggcggtaa aaggcatttt cgccatcacg
    10741 tccgcgcccg aagatttggt gtgccggcgc agcggcgatg ccgtctgaag aagcggcagg
    10801 gcaggaaaac attttaaacg gcgtctgctg ccgatataca taacacggga atcgaaatgc
    10861 tcaaacaatc catcgaaatc atcaacaaac tcggactcca cgcccgcgcg tccaacaagt
    10921 tcacccaaac cgcgtcccaa ttcaaaagcg aagtctgggt tacgaaaaac gacagccgcg
    10981 tcaacggcaa aagcattatg gggctgatga tgctcgccgc cgccaagggt acggtcatcg
    11041 aactggagac ggacggcgcg gacgaggcgg aagcgatgcg cgccctgacc gacttaatca
    11101 acggctactt cggcgagggc gaataatgag tatcgtgctg cacggcgtgg cggcgggcaa
    11161 aggtattgcc gtcggttgcg cccacctgat tgcgcgcggt acggaggaag tgccgcagta
    11221 tgatgttgcg gaggcggaca ccgatgccga agccgaacgt ttcgatgccg ccgtcaaagc
    11281 cacgcgcaaa gagttggaac agctccgcag cgcgattccc gaaaacgccc cgaccgagtt
    11341 gggcgcgttc atctcgctgc acctgatgct cttgaccgat gttaccttgt cgcgcgaacc
    11401 cgtcgatatt ttaagggaac aaaaaatcaa cgccgagtgg gcattgaagc agcagagcga
    11461 caaactcgcc gcccaattcg acaatatgga cgatgcctat ttgcgcgaac gcaagcagga
    11521 tatgctgcaa gtcgtccgcc gcatccacaa caacctgatc gggcagggca acgagttgga
    11581 agttgccgac aacctgtttg acgaaaccgt tctgattgca aacgaccttt cgcccgccga
    11641 cacggttttg tttaaagagc agcgcattgc cgccttcgtt accgatgccg gcggccccac
    11701 cgggcatacg gcgattttgg gcaggagctt ggacatcccg tccgtcgtcg ggctgcacaa
    11761 cgcgcgcaaa ctgattaccg aaggcgaaac ggtcattgtg gacggtatca acggcgtgtt
    11821 gattatcgcg ccggatgagt cggtgttgaa cgaataccgc cgccgtgccc gcgaataccg
    11881 cagccacaaa cgcgatttga acaagctcaa aaaaaccgcc gccgccaccg ccgacggggt
    11941 ctgcatcgag cttgtgggca atatagaatc cgccgaagac gtgaaaccgc tgcacaacct
    12001 cggcgcagac ggcatcgggc tgttccgcag cgagtttctt tacctgaacc gcgatacgat
    12061 gccgtctgaa gacgagcagt acgaagtgta caacgcgatt gtcaaaaaaa tgaagggcaa
    12121 aagcgtaacg atacggacag tcgatttggg agtggataaa aatccgcgct ggttcgggaa
    12181 aaacagcacg cccaacggca gcctcaaccc cgcgctgggc atgaccggca tccgcctgtg
    12241 ccttgccgaa ccggtcatgt tccgcaccca gatgcgcgcc atcctccgcg ccgccgccca
    12301 cggccccgtg cggatgatgt ggccgatgat tacctccgta tccgaagtgc gccagtgcct
    12361 catccacctc gacaccgcgc aacgccagct tgccgaacgc ggcgatgcct tcggtaaagt
    12421 cggcatcggc tgtatgattg aaattccgtc tgccgcgctg accgtcggca gtattttgaa
    12481 actggtcgat ttcatttccg tcggtaccaa cgacctgatt caatacatct tgtccgtcga
    12541 tcgcggcgac gacagcgtca gccatctcta ccagcccgga catcccgccg tgctgaaaat
    12601 gctgcaacac gtcatccgca ccgccaaccg catggacaaa gacgtgtccg tatgcggcga
    12661 gatggcgggc gataccgcgt ttacccgcgt tttattgggt atggggctgc gccgtttttc
    12721 catgaacccc aacaacatcc tgcccgtcaa aaacatcatt ctgcacagca atgtcgggca
    12781 gctcgaaagc gatattgtga aagtcatccg ctgcgaagac gaagagaagt cggaaaagct
    12841 gatcaaacag atcaacagcg tgtctgtcga ggaagaagcc gacttcaaag ggcggaaata
    12901 aatacggcag gtgaaaaata gaaatactta acaatgcccg caatctgaaa ttttgccatt
    12961 cttgcaaaat agaaaaccga aacagaaacc caaaatcggc cattccctca aaaacagaaa
    13021 accaaaatca gaaacctaaa atccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg
    13081 cacggaaact tgtcgggtaa aacggtttct tgagatttta cgttctggat tcccgcctg
//
LOCUS       DAWFJW010000057        12865 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFJW010000057 DAWFJW010000000
VERSION     DAWFJW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.057"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(54..362)
                     /locus_tag="WGR19_001258"
     CDS             complement(54..362)
                     /locus_tag="WGR19_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798790.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(346..648)
                     /locus_tag="WGR19_001259"
     CDS             complement(346..648)
                     /locus_tag="WGR19_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0798791.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(722..3085)
                     /locus_tag="WGR19_001260"
     CDS             complement(722..3085)
                     /locus_tag="WGR19_001260"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0798792.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAGTAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVNEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGSLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAVV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3121..3639)
                     /locus_tag="WGR19_001261"
     CDS             complement(3121..3639)
                     /locus_tag="WGR19_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798793.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3646..4710)
                     /locus_tag="WGR19_001262"
     CDS             complement(3646..4710)
                     /locus_tag="WGR19_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0798794.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="WGR19_001263"
     CDS             complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="WGR19_001263"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0798795.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSGEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6225..6584)
                     /gene="rplT"
                     /locus_tag="WGR19_001264"
     CDS             complement(6225..6584)
                     /gene="rplT"
                     /locus_tag="WGR19_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0798796.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6597..6794)
                     /gene="rpmI"
                     /locus_tag="WGR19_001265"
     CDS             complement(6597..6794)
                     /gene="rpmI"
                     /locus_tag="WGR19_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0798797.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6941..7462)
                     /gene="infC"
                     /locus_tag="WGR19_001266"
     CDS             complement(6941..7462)
                     /gene="infC"
                     /locus_tag="WGR19_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0798798.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7480..9393)
                     /gene="thrS"
                     /locus_tag="WGR19_001267"
     CDS             complement(7480..9393)
                     /gene="thrS"
                     /locus_tag="WGR19_001267"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0798799.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTLEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="WGR19_001268"
     tRNA            complement(9446..9522)
                     /locus_tag="WGR19_001268"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="WGR19_001269"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="WGR19_001269"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0798800.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQAQFHENRARGTD"
     gene            10826..11908
                     /locus_tag="WGR19_001270"
     CDS             10826..11908
                     /locus_tag="WGR19_001270"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0798801.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12100..12501
                     /locus_tag="WGR19_001271"
     CDS             12100..12501
                     /locus_tag="WGR19_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0798802.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12564..12854)
                     /locus_tag="WGR19_001272"
     CDS             complement(12564..12854)
                     /locus_tag="WGR19_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798803.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         3257 a         3391 c         2869 g         3348 t
ORIGIN      
        1 tattttttag cgtgtaaaac aaaaccggct gcatacctgc aaccggcctc aaatcagcac
       61 aattccttat ccaaatccgc caacaggtct ttcagaccgt ctccgatttc ctgaagcgta
      121 accggacggt tgccgtccga acccgattcc gcaccttctg catacggtgc aaacgtgctc
      181 tgcaatttca acagtttcac cacatccaaa cgggtgtagc gttcgccgcc gtaaccgaca
      241 accacaccgt gattatgctg ccattgcgca aaaccatagg ggctgatttg caacagtccg
      301 cacaactcgt ccagcgtgaa atagcgtttt gcgggaacct tcaaatcaac gttgtttgtc
      361 atagtagtgt tccaccatgc ttttgagttt ctggctggca tggaaagtta ccacgcggcg
      421 ggcggtaatc ggcacttcct cgccggtttt aggattacga cccgggcgtt gcggcttgtc
      481 gcgcaactgg aaatttccga aaccggaaat tttgatttct tcgccgcttg ccaaagtgct
      541 gcggatttct tcaaaaaaga gttcgacgat ttctttggca tcgtttttgg tgacgttgct
      601 gactttgtct accaaaatat cggccagttc tgctttagtg agagtcatat gattactttc
      661 tgttgtaaca gttgtggatt attaccaatt tttatttaaa attcaagcga atatttgttt
      721 tttaactgcg aagccgcgcc cctgccgccg ttgccgcgcc aatcagtttt ccgataagcg
      781 gctcgaccgc ctcatccgtc agcgtgtttt ccatatcctg caaaatcact ttgaccgcca
      841 cgctcttcat cccttcgggc aggcccgtgc cgcgatacac gtcaaacacg ctgatttcct
      901 gtaccaactt gtttgccgcg cctttcaaga caagcagcaa atcatcatgg ctcatagctt
      961 ccggcatgac aaacgccaaa tcgcggcgta ccggctggaa tttcgatacg gcccgatagc
     1021 gcgttttccc gcattccaac acaaccgcca tatcgatttc aaataccagc ggcgcttgcg
     1081 gcaggtcgta tttttgcagc catttcggat gcagttcgcc gacaaagccg atgacttttc
     1141 cgtctgaaac gatattggcg gcacgtccgg gatgcagggc gggatgtccg gttttaacga
     1201 actcgactgc tttgtttttc aacagatttt ccacgtccgc cttgatgtcg taaaaatccg
     1261 cattgcgcgt tttcccgccc cattgttcgg gcattgccgc gccgtaccac agactgccga
     1321 tgcgttcgtt ttggacaaac cggccgtctg aacctttgcc gaacacgcgg gcgatttcaa
     1381 acacgcgcac gcggttttgt ttgcggttca ggttgttttg caaaatttcc accaagccgc
     1441 cgatgagtgt ggaacgcatc acggcatatt gcgccgccaa cgggttttgc aggcggatgg
     1501 ggtcggcgtt ggcggcaaaa tcttgttccc actgctcgtt aacgaaggca tagctgacca
     1561 cttcgcggta gccgcgcgcc gccatttcgt tgtaaacggc aaaacgcggg cggcgtgttt
     1621 cgggcagttc cagcattttc agacggcctg acgtgtaatc gtcggggatg ttttcatagc
     1681 cgtaaacgcg tccgatttct tcaatcaaat cagcctcaat ttcgatgtca aaacggaagc
     1741 tcggcgcggt aacgcggaag ccttccgccg ttttctcggg ctgcaggccc aagtgttgca
     1801 aaatggtttc cacctgttcg gcaggaatgt ccacgcccaa cacggttttc agacggtcca
     1861 aacgcaatcc aacctgcttc gcttcaggca attcgccttg cgcttccacc atctcgcctg
     1921 ccgcaccacc gcaaatctgc aacaccaatt cggtagcacg ttcaatggca tccgcctgca
     1981 aacggtaatc cacgccgcgc tcgaagcgga acgacgaatc cgaaccgaaa ccgtattggc
     2041 gcgatttgcc ggcgatgatt tcgggcgcaa accaagccgc ttccagcacg atattttgcg
     2101 tgccgtctga aaccgcgctc gccgcgccgc ccattaagcc cgccaaactc aacacgcctt
     2161 tttcgtccgc cacgaccagc gtgttttcag acagggaaac ggttttctcg ttcaggcatt
     2221 ccagcgtttc cccttcgcgc gcgcggcgga tgtgcaggct gccggaaagt ttgtcggcat
     2281 caaaaacgtg catcggctga ccgatttcca gcatcacata attgccgatg tccaccagcg
     2341 cggaaatact gcggatgccg ctgcgctcca aacgttgttt catccaatcg ggagtagccg
     2401 ctttcgcatt gacattttca ataacgcggc tgataaaacg gccgcaatcg gcaggcgcgt
     2461 taatctgcac gggctgtttt cgactgcccg tgatcggcgc ggtatggatt tcgggctgcc
     2521 tgaacgcgca ccccgtcaat gcggacactt cgcgcgcaat gcctttgatg ctcaagcagt
     2581 cggcgcggtt aggcgtaatt ttcaacgtaa acagcgtatc gtccaaatcc aagtattcgc
     2641 ggatattggt accgacgggc gcatcttcag gcagaatgtg caggccgttc acaccgtcgt
     2701 cgggcagacc gagttcgtcg gtggaacaca acatcccgtc cgacaccacg ccgcgcattt
     2761 tggtcggctt gattttgaaa ttacccggca aaacggcacc cggcaacgaa cacggcactt
     2821 tgatgcccgc tttcacattc ggcgcaccgc acacgatctg aaccaactct cccgtacccg
     2881 catcaactcg ggtaacgttc aaacggtctg catccggatg tttttcaacg gatttcactt
     2941 cggcaacaac cacgcccgca aaggcaggtg cggcagtccc ggcttcttct acttccaagc
     3001 cggacatcgt taacaggtgt tccagcttat cggcggaaag ttcggtatcg gcttgggttt
     3061 tcagccatga gtaggagaat tgcatgttat gttctcaatt aactaattaa ctttagattt
     3121 tcaagagcta gctaaacctt tttgaatggc taagtcccta ttaattactt ctacagaaat
     3181 gttccataaa gaaatcaacc gcaaagtatc tttcttatta aattcttgct ttattaattc
     3241 attcaattgc ccaagataat taagcggcac atccgggata accagtaata atttgatttc
     3301 tatgctaccc cattgcaaat ttgaaaaagc actaggcata attgtttctt tgatatcttc
     3361 tctgcctaat attcccaaaa tttcaacatt taatgcatta caccattttt gataaatctc
     3421 tgaaatatat tcatcaaaac gctcaggaga tctttcttga tttggaatac tttgttttgc
     3481 ctcaacaaat aacaaattat cttcttgcag atagataaac tctactgtct taataccttc
     3541 ttgccttctc ttccggatat atggttctat ataaaacagg ttatttttta ctagattaaa
     3601 agttatacct gattcagtga gatttattat ttcactcata aaaacctaac caatccatgc
     3661 tgtttccgga gataaaacac ctatttcttc tctgaacaat cgaatagact caccaataat
     3721 ttcattatcc ggcatgctat tttgtaaatt ttcctgcacc caagtagtct gttccttttc
     3781 tttgccaggc ataaaaacag gaatactttc actatgcttt ctagctaata aacacagctt
     3841 cttgataaca aaatatgaat gcgtactcat aaaaatctgt attcccaatt tggatttaga
     3901 taaggaaaac agaatttcta gaaattgatt aattgctttc ggatgtagat tagcttctgg
     3961 ctcatcaata aataagattg atccaggact caaaaaccga tttcctaata gagtgtctaa
     4021 tatcccaatc ttttttaccc cctctgctgt agaattgata gagaaagttt gtctatcttt
     4081 ttcatatacc catgccttct ttttatcatt gtagctaaca taaccgccaa acaatgcttc
     4141 tagatccttt ctagcttccg ctgcctcagg gtaattcctc ccttttgttg gtttaatatc
     4201 caatgctttt accaaatcaa tgtaagtagc atcaaaacca aatctctttt cttcctctga
     4261 ttttttaatt acatcaaata gtgatagaac ttctttgggt ggtaaaaaaa tagagttagc
     4321 gtttattctg ggagaaagtt cgtatagctc cggtttaatt agctttttgg tagttctacc
     4381 aaaagaaaac aaaactgaag aactatcttc taaagtcata cttaacttaa gaggccgttc
     4441 gtttggattt tcttttcctc ttgtaactaa atcaccaatt tcctctactt ggaacgtcca
     4501 atatagctta tctcgcaatt cctcagaaaa atcacgaata ttctttcctt tatgagcttc
     4561 ctcatgactt tttaaaacac tatataatgc ctttaaaata aaagttttcc ctttgctatt
     4621 ttctgcaata attaaattta ttttccctaa attatcagcc ttcaataagc taatcggccc
     4681 gaaattttca agccaaaatg atttaatcat ataatccccc taatatatat caacaatatt
     4741 tgtacaagac acattgtgaa aagacagcaa ccacagcacc atagtaaacg catagcaggc
     4801 ggataatgga gcgacatagc gtttcatgat ttagctttct gaggtaggca caggctgctt
     4861 ttttcatttt taggcagcct acacaatcat tttacaaact gcttcaaaaa attcaaatcg
     4921 ttatcaaaaa acaagcgcaa atcgttcacg ttgtaacgca gcatcgcgaa acggtcgaga
     4981 ccgataccga aagcgaaacc ggtgtatttt tcgggatcga tattcacatt tctcaacacg
     5041 ttgggatgca ccataccgca gccgccgact tccagccatt tgccgttttc gcccataatg
     5101 tcgatttcgc cagacggttc ggtgaacggg aagaaagacg ggcggaaacg tacttgcaaa
     5161 tcatcgcgtt caaagaagcg acggataaaa tccgtgaaca ctgctttcaa atcggcaaaa
     5221 gttacgccct cttctaccca caaaccttcc gcctgatgga acataggcga gtgcgtggca
     5281 tcgctgtcca cacggtaaac gcggccgggg gcgataatgc ggatgggcgg ctctttttta
     5341 tcgagcatat agcggatttg aatcggggaa gtgtgcgtac gcaaaacatc accgttttca
     5401 acgtaaaacg tatcctgcat cgcacgggca ggatggtttg cagggatgtt cagggcttgg
     5461 aaattgtgaa aatcgtcttc gatttcaggc ccgtccgcca cttcgaaacc cattccgtga
     5521 aagagttcga ccacacgttg caaggtcagg gttacgggat gcaggctgcc gccttcctga
     5581 gcgcgtccgg gcagggtaat gtcgagggct tcggcggcaa gtcgggcttg cagcttggct
     5641 tcgttgaggg catcgcgttt ggcattaaaa gccgtctgaa accggttttt gcattcattg
     5701 atatgcgcac ctatggtttt gcgctcttca ggcgacattt gccccaaagt tttcagaagt
     5761 ccggtcaact cgccggtttt gccaagataa cgggctttga tttgttctag agcgttgaag
     5821 tcttgcgcag cttctactgc ggcaatgcct tctgcaacga tgcggtttac attttccata
     5881 atatcaagtc tgtcaattaa tgatatgcca cctgaaacaa cgaggccgcc tgtatagcct
     5941 gacatatcag actcttcaga cgaccttatt ttcttcacgg tcaatccatt ggataaattt
     6001 tctgtatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc gtactatctg
     6061 tactgtctgc ggcttcgtcg ccttgtcctg atttaaattt aatccactat attttaacgc
     6121 aaatttattt aaaaaaatag actttaaaga aagaaaatca atcacataga atttctgcaa
     6181 acaaagaaaa aaggaagccg cagcttcctt ttcaattttt tggattaagc agccaaagca
     6241 gctttggctt tttcaaccaa ttgtgcaaaa gcggctttat cgaacacggc caaatcagcc
     6301 aatactttgc ggtcgatttc aatagaggcg cgtttcagac cgttcataaa tttgctgtaa
     6361 gacaacccgt tttcacgcgc acctgcattg atacggacaa tccacaattg acggaattgg
     6421 cgtttgcgtt ggcggcggtc acggtatgcg tattgaccgg ctttcattac cgcctgtttg
     6481 gcaacgcggt aaacgttttt acgacgaccg cgatagcctt tggctaacgc gaagattttt
     6541 tggtgacggg cacgggcggt aacaccgcgt tttacgcgtg gcatattcta aactccttaa
     6601 gcgtagggta acattttagc aacagaagcc aaatcgcgat catttaccat agaggtaccg
     6661 cgcagttggc gtttgttttt ggtggtcttt ttagtcaaga tgtggcgttt gaacgcatga
     6721 gcgcgtttca caccgccgtt acccagtact ttaaagcgtt ttttcgcgct agacttggtt
     6781 ttcattttag gcatgggaaa actccattcg ttatcggata aggcattagg gggttttaaa
     6841 acatcggttt caaacacttg aacccacaat accacggttt tttgccgcaa ttgaaaactt
     6901 actccgaagc ggcaatcgga gtaagcggag aattatagct ttattttttc ttcggcgcaa
     6961 tcatcatcac catttggcga ccttccattt tgggaaagga ctcgatttgc gccacttcag
     7021 ccaaatcttc ttttacacgt tccaaaagtt gcgcgccgag ttgctggtga gccatttcac
     7081 ggccgcggaa acgcaatgtc actttgactt tatcgccgtc ggcaaggaag cggttaatgt
     7141 tgcgcatctt gatttgataa tcgccctcat ccgtacccgg acggaatttg atttccttga
     7201 tttgcacctg cttttggttt ttcttggctt cgtcgcgttt cttggcctgc tggtatttgt
     7261 atttaccgta atccatcagt ttgcacacag gcggtttagc agttggggaa atctctacca
     7321 aatcgacatc ctgcccttcg gccatagcca aagcttcacg aactgaaacg acaccaagct
     7381 gttcgcctga ctcactgatt aaacgcactt ctttggcggt aatttcgccg ttgattcgtg
     7441 cttcgcgttc ttgagcgatg atgaatactc ctataaaaat taatgattga cgagggcatc
     7501 agtgatttct tgctgcaatt gcgcaatgaa atcatccaaa tccaaagaac ccaaatcttc
     7561 tgctttgcgg cgtaccgcca ctttgttttc ctgcttttct ttatcgccga caacgatttg
     7621 ataaggaaaa cggtattggc tgttgtcgcg gattttgtaa ccgatttttt cgttacgcaa
     7681 atccaactcg gcgcggaatc ctgccgcctg caatttggca gccacttcac ggcagtaatc
     7741 cgcttgattt tcggtgatat tcataatcac catttgaacc ggcgccagcc ataacgggaa
     7801 agagcctgca tggttctcaa tcagaatgcc gataaaccgc tccaaagaac ccaaaatggc
     7861 gcggtgcaac attacagggc gagcgcggtc attgttttcg gttacatatt ctgcatccaa
     7921 gcgttccggt aagacgaaat ctagttgcaa tgtaccgcat tgccaagaac gacccaaggc
     7981 atctttgacg tgatattcga ttttaggccc gtaaaacgca ccttcgccag gcaactcttc
     8041 ccactctacg ccacaagcgg tcaatgcatc acgcaagcct tgttcggcct tatcccaaac
     8101 ttcgtcagaa cctgcacgtt gttcagggcg gagggacaac ttcacagcaa catcatggaa
     8161 accgaactgt ttgtagatgc gaaccaacaa ttcattgaac gcacgggcct cgctgacgat
     8221 ttggtcttcg gtacagaaaa tatgcgcatc atcctgcaca aatccacgga cccgcatcag
     8281 accatgcaat gcaccgcttg gttcattgcg gtggcaagaa ccgaattccg ccaaacgcat
     8341 cggcaaatcg cgatatgagc gcaaaccgtt attaaaaatt tgcacatgac ccggacagtt
     8401 catcggttta accgcatatt cgcgtttttc cgaactggtt acgaacatat tgtctttgta
     8461 gttgtcccaa tggccggatt tttcccaaaa ggttttatcc ataatttgag gcgttttgac
     8521 ctctttataa ccggcggcgt tcagctcttt acgcatatgc tgttcaatca cttgccacaa
     8581 agcccagcct ttaggatgcc aaaacaccat gcccggcgct tcgtcttgca gatggaacaa
     8641 atccaattgc ttgccaagtt tgcggtggtc gcgcttttcg gcttcttcga tacgttgaat
     8701 ataggctttc aactcgtctt ttgtcgccca agccgtaccg tagatgcgtt gcagcatttc
     8761 attgttgctg tcgccgcgcc agtatgcgcc cgccagcttg gtcagtttga agtttttcag
     8821 gaaacgagta ttcggaacgt gcggtccgcg gcacatatcg acatattcct ggtgatgata
     8881 catccccatc gcttccactt cgggcatatc gtcaatcagg cgcagtttgt attcttcgcc
     8941 gcgctcttga aaaattttaa tcgcctccgc acgcggagtc atgattttga ccacatcata
     9001 gtcttgggca atcaattctt tcatacgcgc ttcaatggcg gcaacatctt ccagtgtaaa
     9061 cggtttttcc gtggcgatgt cgtaataaaa gccctcttca atgacggggc cgataaccat
     9121 ttttgcatta ggatagagtt gcttgacggc atgcccgaca agatgggcgc aggaatggcg
     9181 gatgatttcg atgccttcct gatctttcgg agtaatgatt tgaacagcag aatcttcaac
     9241 aatcgggtcg cacgcatcga ccaatttgcc gtttaccctg cctgccaccg tcgccttcgc
     9301 caaaccggca ccgatagacg cagcaatttg agccacggta acgggggatt cgtattggcg
     9361 gactgagccg tccggcaagg taatattcaa catcaaacgc tccgaaagat taaaaaataa
     9421 cggcataaat gccgttatgg ggatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcatt tctttatgca acattgtggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
     9661 acatatttcg ccccctgtcg cccaactgac attcaatctt atgcttaaat ttactttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaattgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ctcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 tttggaagtt atcgggcaat tcggcggcct gcacggtttt atcggctggg acaaaccgat
     9961 tttgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgttcaaaa gcccgattaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttctatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagttgg acgaatgcac
    10141 gccgggcgag gcgacgcgcg aacaggcgcg aaaatcgctg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcactgt tcggcatcgt
    10261 gcaaggcgcg atgtatgagg atttgcgcga agaatcgctg cgcggtttgg aacagtttga
    10321 ttttcccggc cttgccgtcg gcggtttgtc cgtcggcgaa cccaagcccg aaatgtaccg
    10381 tatgctgcgc gccgtcggtc cgattctgcc ggaacacaag ccgcattacc tgatgggcgt
    10441 aggcacgccg gaagacctcg tgtacggcgt ggcacacggc atcgatatgt tcgactgcgt
    10501 gatgcccacc cgcaatgcac gcaacggctg gctgttcacc cgtttcggcg atttgaaaat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg
    10681 cgcacagctc aacaccatcc acaacctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgatgccata gaacaaggca aatttgccga ctggcaggca caattccacg aaaaccgcgc
    10801 acgcggcaca gactgattcc cggcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattcc ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgtt
    11101 gcgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgcccct
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccgaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggcgcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acggacgtgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattctg cccgggcttt ttggcagact gtttggaaac
    11761 catggaagaa atcgccctga tggggcggga acagttttac gaagcaggcg gcaaaagcta
    11821 ccgctacatc ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgccgga
    12001 caacaacatc aagtgcaacc ttactaccga ttcggcaaaa aaatctttca cccgctacgc
    12061 aaaatcaaca acaaggtttc aaacattaag gaaacaccaa tgaacacaac ccgactgccg
    12121 accgccctcg tcttgggctg cctctgcgcc gccgcttctg ccgccgacaa cagcatcatg
    12181 acaaaagggc aaaaagtgta cgaatccaac tgcgtcgcct gccacggcaa aaagggcgaa
    12241 ggccgcggaa ccatgtttcc gccgctctac cgctccgact tcatcatgaa aaaaccgcag
    12301 gtgctgctgc acagcatggt caaaggcatc aacggtacaa tcaaagtcaa cggcaaaacc
    12361 tacaacggat tcatgcccgc aaccgccatc agcgatgcgg acattgccgc cgtcgccact
    12421 tatatcatga acgcctttga caacggcggc ggaagcgtta ccgaaaaaga cgtaaaacag
    12481 gcaaaaggca aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
    12541 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
    12601 ccgccaccgt caacctcctt cgcggtcagc ataaagccgc cgatgtcaat acggccgcac
    12661 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
    12721 tcgcccgctt ccagcctcaa accgtaagca agtgccaaat cacccgaacg tgctgccggc
    12781 gaaacgacaa actcgccgaa aaaatcaaaa tgctcacgga caccgatacc cgtctcggta
    12841 aagtatttcg ccattttata gtgaa
//
LOCUS       DAWFJW010000058        12829 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFJW010000058 DAWFJW010000000
VERSION     DAWFJW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.058"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(372..1412)
                     /gene="mutY"
                     /locus_tag="WGR19_001273"
     CDS             complement(372..1412)
                     /gene="mutY"
                     /locus_tag="WGR19_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0798804.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQILAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            1575..2520
                     /locus_tag="WGR19_001274"
                     /pseudo
     CDS             1575..2520
                     /locus_tag="WGR19_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(2532..2762)
                     /locus_tag="WGR19_001275"
     CDS             complement(2532..2762)
                     /locus_tag="WGR19_001275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798805.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKMPIRLPHNKNN
                     GQFIRQQPDSPFYAAEKMPSETHSDGIRLKNN"
     gene            complement(2777..3415)
                     /locus_tag="WGR19_001276"
     CDS             complement(2777..3415)
                     /locus_tag="WGR19_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0798806.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAS
                     HNSGIPLIPGVATPSEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(3596..5431)
                     /gene="edd"
                     /locus_tag="WGR19_001277"
     CDS             complement(3596..5431)
                     /gene="edd"
                     /locus_tag="WGR19_001277"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0798807.1"
                     /translation="MNTTPIHSKLAEITGRIIKRSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            5947..7392
                     /gene="zwf"
                     /locus_tag="WGR19_001278"
     CDS             5947..7392
                     /gene="zwf"
                     /locus_tag="WGR19_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0798808.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTAGFLAKAETNSKIHIKQNFSDEAWASFVKRIEYLKVDVTEAADFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            7672..8367
                     /gene="pgl"
                     /locus_tag="WGR19_001279"
     CDS             7672..8367
                     /gene="pgl"
                     /locus_tag="WGR19_001279"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0798809.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            8348..9334
                     /locus_tag="WGR19_001280"
     CDS             8348..9334
                     /locus_tag="WGR19_001280"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0798810.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            9384..10232
                     /gene="hexR"
                     /locus_tag="WGR19_001281"
     CDS             9384..10232
                     /gene="hexR"
                     /locus_tag="WGR19_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0798811.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            10353..11999
                     /gene="pgi"
                     /locus_tag="WGR19_001282"
     CDS             10353..11999
                     /gene="pgi"
                     /locus_tag="WGR19_001282"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0798812.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELACEAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            12013..12564
                     /locus_tag="WGR19_001283"
     CDS             12013..12564
                     /locus_tag="WGR19_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0798813.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         3106 a         3244 c         3522 g         2957 t
ORIGIN      
        1 attttgtttt aaaagccgat aaatttttac gaaaatttta cattaattgt tatttgcaga
       61 aaattagaac ccgttatcac gtcttggata aatgccaaaa ccattgtgat tcagagaatt
      121 aaaaagaaaa atttcggaat cctcatcctt ttctgaaacc tgattgcggt atccatggct
      181 ttgtctttgt catttaagtt tgtgttcatg tttttgacct tctttgcttt ttaaatcctt
      241 tttaaattgc cgacttcttc cgttccaaat gtcccatttg aacattatcc cgccgcctaa
      301 aatacgaccc aaattcatcg gttgttcttg ctgcgatacc tgcaaacaac agttcaatgg
      361 gtttattttg tttattctaa cctattgccg tttaaataaa tttccaaagg cttgggcagg
      421 ccgtaatctt tcaaatgcgc cggctttatc caaatgccgt ctgaagggct ttcagacggc
      481 atttgtcctt caaacggcgt aatcatcagc agccggtgcg tcaggcggtg ggtcagggcg
      541 gtttgttcgt ccatatctgc catggtcagg gagaatttgg cggcaaagtc ggaaagcccg
      601 ttcaaacttt caaaacacgg tacgcaatac agaccgcccc aaatgccttt ggcggggcgt
      661 ttttccagca aaatcgcgcc gtttcggttg cggataatca gccagtaaag cggcagggtc
      721 ggtacttcgg cggcggtttt tttgcgcggc agctcggcgg tgcggttttg cttttttgct
      781 tcgcagatgt ccgccatcgg gcattggtgg cacaagggtt tcgtccgttt gcacacggtc
      841 gcgcccaaat ccatcaaacc ttgtgtatag gcaggcatat cggcgttttc agacggcagc
      901 aggctttcgg caagtgtcca gagcgagttt tcaaattttt tgtcctgcgg attgccgtcg
      961 cgggcgaaca cgcggcagag cacgcgtttg acgttgccgt ccaaaatggt ttcgcggcgg
     1021 ttgaaggcga aggcagaaat ggcggcggcg gtgcttctgc ctacgccgca gagcgtttcc
     1081 aagtctttac gctccgatgg aaatgtgccg ccgaattgtc cgacgacttg ttgcgcggct
     1141 ttgtgcagat tgcgcgcgcg gctgtaatag cccaagcctg cccacaagga taaaacctcg
     1201 tcttgcggcg cggcggcaag tatttgaaca gtcgggaatt tttctaagaa acgtggatag
     1261 tagtccaaca cggtggcgac ctgcgtttgc tggagcatga tttcggaaag ccagacgcaa
     1321 taagggtttt tgacctgcca agggaggtgg tggcgaccgt gttgtttttg ccagcggatg
     1381 agccgttcgg agaaggggat gggtgtgttc attaatatca atcggtggtt ttatttatat
     1441 ttaaaacagt atgttattgt ataacattgt gaaaataatt cttattgact tattttttgt
     1501 aggggcacat aactcatata aagaaacttt attgcggtat tgaaattatt tatcaacaag
     1561 caaggagtat cagcatgaaa gcaatgcttt atcacggcgc aaacgacatc cgttttgaag
     1621 aaaaaccccg tccgcagatt atcgatccga ccgatgcggt ggtgaaaatc gtcaaaacca
     1681 cgatttgcgg taccgatttg ggtatttgga aaggcaaaaa aacccgaagt cgccgacggt
     1741 cgtattctcg gtcatgaagg catcggtatt gtagaagaag tcggcgaggc tgtaaaaaac
     1801 atcaaagtcg gcgataaagt cattatttca tgcgtcagca aatgctgtac ttgcgacaac
     1861 tgcaaaatcc aactttattc gcattgccgc aacggcggct ggattttggg ctacatgatt
     1921 gacggcacgc aggcagaata cgtccgcacg ccttatgccg acaacagcct cgtgccgctg
     1981 cccgacaacg tcaacgaaga agtcgccctg ttgttgagcg acgccttgcc gaccgcccaa
     2041 ctgtacagcc ccgccgccat cattgtttgc gatatggacg aaaaccgttt gaaactggcg
     2101 aaagagttgg gcgcaaccca taccattaac cctgcttccg gcgatgtaag caagcaagtt
     2161 gccgctatcg tcggcgaaga cggcgtggac tgcgcgatcg aagccgtcgg tatccctgca
     2221 acttggaata tgtgccaaga cattgtgaaa cccggcggcc atatcgccgt tgtcggcgta
     2281 tacggtcaat ccgttgattt caaactggaa aaactgtgga ttaaaaagct tgccatcact
     2341 accggcctgg taaacgccaa taccaccgaa atgctgatga aggcaatttc cagcagctcc
     2401 gtcgattaca ccaaaatgtt gacccaccgt ttcaaattca gcgaattgga aaaagcctac
     2461 gacgtgttca aacacgccgc cgaaaatcaa gcgatgaaag tggttttgga agcggattaa
     2521 tcggccttga atcagttgtt ttttaagcgg atgccgtctg aatgcgtttc agacggcatt
     2581 ttttcagctg cgtaaaacgg gctgtccggc tgctgtcgaa tgaactgacc gttattttta
     2641 ttatgcggaa ggcggatggg cattttaggg gcggtatgtc tgatcagtgc tgccgtgtat
     2701 gccgactgtg ccctcaatcg gcaaaatgcc gtctgaaacg ggaaaggttt ttcagacggc
     2761 atttttacga tgcgaatcag gctttgggtt tcaacgccgc cgcttctttg gcgaggcggg
     2821 tgatggtgtc ccagtctttg tttttcacgg cttctttcgg tgtcagccaa gagccgccga
     2881 cgcacaggac gttgggcagt gccaggtatt cgggggcagt tgccaggctg atgccgccgg
     2941 ttgggcagaa gcgaacatcg gcgtaagggc cgtaaagggc tttgagcatg gctttgccgc
     3001 cgacgacttc ggcggggaag agtttgaggg tgtcgatgcc gtgttccaag gccagttgga
     3061 cttcgctcgg agtggcaacg ccgggaatca gggggatgcc gctgttgtgg ctggctttgg
     3121 cgagggattc gtgcagcccc gggctgatgg cgaaaaccgc gcctgcgtct tcgacggctt
     3181 tgagctgttc gggattggtt accgtacccg cgccgacgat ggcgttgggt acttctttgg
     3241 caatcaggcg gatggcatcg agtccgacgg gtgtacgcag ggtaatttcg agggtaggga
     3301 tgccgccttc gacaagggcg tgggacaaat cgatggcggt gcttaagtcg tcaatcgcca
     3361 ttaccggcac aactgcgccg gcggtcaaaa tttcgcgggg ggtcagtttg gacatttcgg
     3421 ttctccgggt ggaatggggt attttattaa gatgggacag gttgtaggtc ggattcaaga
     3481 aaccgacatc ttggacggct gtgtcggatt cgagaatcca acctacgtct atcttaaaac
     3541 acttgaataa tttttcagac ggccttcggt ctgaaaaggc cgtctgaaac gcgcatcagg
     3601 caaattcgcc gccgaagctc atcgcgccgg tttcggcgct gctggtcatg ctgcggaagt
     3661 tggcgaaaag ttcgcggccg cagccttgtt ggttcgcgcc caagtcgatg ctttcgactt
     3721 cgcgggcatt ccattcggtt tcgttaatca ggacgttgag ttcgccgcta acggagtcga
     3781 agcggatcag gtcgccggta cggattttgg cgatgttgcc gcccatcagg gcttcgggcg
     3841 tcatgtggat ggaggccgga actttgccgg atgcgccgga catacgaccg tcggtcagca
     3901 gtgccacttt gaagccgcgg tcttgcagga tgcccaaagg cggggtcagt ttgtgcaatt
     3961 cgggcatacc gttggcacgc gggccttggt agcggacgac gcacacaaaa tcgcgttcca
     4021 actcgccgcg ttcaaacgca gccaacactt cgcgttggtc gttgaacacg atggcaggcg
     4081 cttcaataat gcggcagcct tcgcgcacgg cggacacttt aatcacgcca cgtccgatgt
     4141 tgcctttcat caggcgcaga ccgccgtcgg gggagaacgg gttgtcagct ttacgcagga
     4201 tgtcgtcgtt gccgctggtt tcgggggctt cgcgccattc gagtttgccg tcgatgagga
     4261 aaggctcttt ggtgtagtgg cgcataccgt gtccgacgac ggtatcgaca tcgtcgtgca
     4321 acaggcctgc attcagcaat tcgcggataa cgaaaggcag tccgcccgct gcggtaaagt
     4381 gattcacgtc ggccttgccg ttcggataaa cgcggatgag tagtgggatg atggaggaaa
     4441 tttcgtcgaa gtcgtcccag ttcaaaatca cgccggccgc acgcgccata gcgacgaggt
     4501 gcatggtgtg gttggtcgaa ccgccggttg ccatcaggcc aatcaaggcg ttgataaagg
     4561 atttttcggt caacatttcg cccaaaggtt taatcgtgcc gtttttgatg ccgcgcgcga
     4621 ggtgtccggc ggcgtagcgg gtcagcgctt cgcgcaggtc ggtgtaaggg tggacgaagg
     4681 cggcggcagg caggtgcacg cccatcattt ccatcatcat ttggttggag tttgccgtgc
     4741 cgtagaaagt gcaggtgccc gggctgtggt aagaacccat ttcgcttttc aaaagttcgt
     4801 tgcgtccgac cttgccttcg gcgaaaagct ggcgggtgcg ggctttttct ttgttgccga
     4861 taccactgga catcgggcct gcggggacga agatacccgg aatatgaccg aacgaaagcg
     4921 cgcctatcat caaacctgga acgattttgt cgcatacgcc cataaacagg ctgccgtcaa
     4981 acatttgatg cgacagcccg acggcggtac tcatggcaat cacgtcgcgg gagaacagcg
     5041 acagttccat accggcgtag ccttgcgtga tgccgtcgca catggcgggt gtgccgccgg
     5101 cgacttgggc ggtcgcgccg tttttctgtg cttcgtcttt aatttggtcg gggaagtctt
     5161 taaagggctg gtgtgcggaa accatgtcgt tgtaggcggt gatgatgcct aagttgggga
     5221 cggtttcctg aagcatttcg attttgatgc ttttgggcat agaggcatag ccgtgcgcca
     5281 agttggagca gccgagctgg ttgcgctcta agcgtcccat ttgtttggca ctgcggatct
     5341 tcgccagata tttttcacgc gtcggacggc tgcgtttaat aatgcgcccg gtgatttcgg
     5401 cgagtttgga gtggatagga gtagtgttca cggtcggtct cctgtcggaa cggggggtat
     5461 tggggatttg tttgtgcgtt tctgattttt gtatcgggta ttcagacggt gagtatagtg
     5521 gattaacaaa aaccagtacg gcgttgcctc gccttgccgt attatctgta ctgtctgcgg
     5581 cttcgtcgcc ttgtcctgat ttttgttaat ccactatatt ttaaaaggta taccgtctga
     5641 aagtgggaag cgggtgttat tatagtacaa aatttgtaat tttgttacaa aaaaatcaga
     5701 catcgaaaag aaatttgatt taaatcgcag aatctttcga ttgttgtaac ggaatagggg
     5761 aaacctgccc gatttttgga ctaggtcaaa taaattgtaa aaatgggaat agacaaaaag
     5821 tgtagcggtg tgtagtatta ctactcatta tgccgcctgc ctttgtttcc gatattgcga
     5881 tgcagcgtga tggggaaaac aggtttcaga cggcatcgaa aaattctttc ctgacgagat
     5941 aatgcgatga gtacacaaac aaattttgat ttggtgttgt tcggtgcgac cggcgatttg
     6001 gcaatgcgca aacttttgcc ctgcctgtat caggcgcacg tggccggttt gcttcatccc
     6061 gaaggtcgta ttttgggtgt gagccgcagc gaattggata ccgccggttt tttggcaaaa
     6121 gcggaaacga attctaaaat ccatatcaaa caaaattttt cagatgaagc gtgggcttcg
     6181 ttcgtcaaac gcatcgaata cctgaaggtt gatgttaccg aagcagccga ttttgccgct
     6241 ttgggcgatt tggtcaaagc gcgcaaagaa accgacaacg tcgtcatcta tctgtccacc
     6301 gcgcccaaat tcttcgcgca agcctgtgaa aaccttgctg cgatcggttt gaatgccgac
     6361 aatgtgcgcg tagttttgga aaaaccgttg ggtacggatt tggcatcttc ccaacaaatc
     6421 aataccgatg ttgcgcgtta tttcaaagaa gggcaaatct accgtatcga ccattattta
     6481 ggtaaagaaa gcctgcaaaa cctgctggcc ttgcgttttg ccaatgtgat gttcgagccg
     6541 ctttggaaca ataaatacat cgaaagcgtg cagctgacca tcgccgagca gttgggcgtg
     6601 gaagaacgcg gcgagtttta cgacattacc ggcgcgttgc gcgatatggt gcaaaaccac
     6661 ttgatgcaaa tgctgtgcat gactgcgatg gaagcccccg ccagcttgga tgccgacgcg
     6721 gtgcgcgatg aaaaagtcaa agtcatcaag tcattgaagc cgctgaccgt cgaatctgtc
     6781 aatgaaaatg tcgtgcgcgg acaatatacc gccgccaaag gcatgaacgg ctatcttgaa
     6841 gaaatcaacg ttccgcaaga cagctttacc gaaacctacg tcgccattaa agccgaaatc
     6901 gaaaacgaac gctggaaggg cgttcccttc tacctgcgta ccggcaagcg catggcgggc
     6961 aaagtggcgg aaatcgtttt gaacttcaaa gatttgaaca gccatatttt tgaaggcagt
     7021 cgcaccgcgc ccaaccggct cgttatcgag ttgcaaccat atgaatccgt gcgcctctat
     7081 acgcagatga aaaccccggg ggcaggaaat aaggtcgaaa ccgtgccgct ggcaaccgat
     7141 ttgggcaaag cattggaaag ccgccgcgcg gaagcttacg agcgcctgct gctggatgtg
     7201 attaacggca aactcgcttt gtttaaccgc cgcgacgaac ttgaagccgc gtgggaatat
     7261 gtgatgccga ttttggaaaa ctggacaaat aacaccacgc cgccgcacgg ctacggcgca
     7321 cactcgtggg ggcctgaagc cgcgcgcgaa ctattggcgc gcgacggaca caagtggcac
     7381 gaagagcagt aatacaataa tgcgttcaga cggcatgggg tttgaaatgc cgtctgaacg
     7441 taagtaaagc agtatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
     7501 tacaaatagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactataaa
     7561 aagcagtccc gatatttggt atcaaaagca taaacaactg ttcggccgac atattgctca
     7621 acccgtttcc ataacggaat atgccgtctg aaataaaaaa aggacacaaa tatgtttgtt
     7681 tggcacgaat acgaaaatgc ggcagaagcg gcgcagtctt tggctgacgc agtggcggat
     7741 gctttgcagg gcgcactgga cgagaagggc ggtgcggtgt tggcagtttc cggcggacgt
     7801 tcgccgattg catttttcaa cgccctgtcg caaaaagatt tggattggaa aaacgtcggc
     7861 atcaccttgg cagatgaacg catcgtgccg accgtccacg ccgacagcaa taccggtttg
     7921 gtgcgcgaat acctgttgaa gaacaaagcg gaagcggcaa tgtggattcc tatggtggaa
     7981 gacggaaaaa ctgaaaccga attacatccc gatgctgttg tcgattatgc actgaaacat
     8041 tacaaacagc ccgatgtttt ggttttgggt atgggaaacg acgggcatac ggcttcgatt
     8101 ttcccgaaag ctccgcagtt tcagacggca atcgacggtt cggcaggtgt cgcgctggtg
     8161 cataccacgc ccgttaccgc gccgcacgag cgcgtcagta tgaccttgga tgcgattgcc
     8221 catacggggc atgtgttttt ggcgatacgg ggcgaagaga aaaaagccgt gttcgaccaa
     8281 gccgcacaag gcgaaaaccg cgaatatccg atcaacctcg ttttgaacca tcaaggagtg
     8341 aactgccatg tcttctacgc cgaataaaca agccggatat ccccgactgg tcgccgatat
     8401 cggcgggacg aatgcacgct ttgcgctgga aaccgcgccg cgcgtcattg aaaaagccgc
     8461 cgtgcttccg tgtaaagact acgatacggt taccgatgcg gtgcgtgcct atctgaatca
     8521 aagcggtgca acagccgtac ggcacgcggc atttgccatc gccaacccga ttttgggcga
     8581 ctgggtgcag atgaccaacc accattgggc gttttccatc gaaaccaccc gtcagacttt
     8641 ggggctggac accctcatcc ttttgaacga ctttaccgcg caggcattgg cggtaacgca
     8701 gacttcaagc aaagacctga tgcaggtagg cgggcaaaag cctgtcgaat ttgcccccaa
     8761 agccgttatc ggccccggta ccggcctggg cgtgagcgga ttggtgcaca gccacgcagg
     8821 ctgggtggct ttggcgggcg agggcgggca taccagtttc ccgccgtttg acgatatgga
     8881 agtgctgatt tggcagtacg ccaaaaacaa atacggccat gtttccgccg aacgcttttt
     8941 gagcggcgcg ggcttgagct tggtttacga ggctttggct gcaaaacaga aagccaaacc
     9001 cgccaaactg atgccgtctg aaatcacgga aaaggctttg agcggcacgt cgcctttgtg
     9061 ccgtcagact ttggacatct tctgcgccat gctcggcacg gttgcttcca acctcgccct
     9121 gacgctgggc gcgcgcggcg gcgtgtacct gtgcggcggc attattcccc gcgtgttgga
     9181 atacttcaaa acttccccgt tccgcagccg tttcgagaac aagggcaggt ttgaagcata
     9241 tcttgccgcg attcccgtgt atgtcgtctt gagcgagttt cccggaattt ccggtgcggc
     9301 tgcggctctt gacaaccatt tgagaaacgt ttaaccacag cggctccttg cagcggggct
     9361 gcattatcga agggcatatc attatgttaa gcaaaatcag cgaatcactg gcaaaccttt
     9421 ccggtgcgga acgcaaagtc gccgaatgtg cattggcgga acccaaatgg ttcgtccatg
     9481 ccgccgttgc cgaaattgcc gaacgtgcgt ccgtcagcca gccgaccgtc atccgattct
     9541 gccgcagctt gggttataaa gggctgcccg agttcaagct cgccttgtcc gccagcatcg
     9601 gtcatgaggg tatgccctat gtccacgaag aactcaacgc cgacgacgat atggcaagcg
     9661 tggtcgagaa agtgttgggc aatgccgccg cctcgctgtt gggcgaacgc cgcttcctga
     9721 aagagtcgga gctggaaaac gccattgcca cgctgatgca cgcccgtcgc gtcgagtttt
     9781 acggtgtcgg caattccggc attgtggcac aggacgcgca gcataaattt ttccgtttcg
     9841 gcatgtccac cgtcgcctat gtcgatacgc acacgcagct gatggcggca tctgttttga
     9901 gcgatcagga tgttttggtt gccatttcca acacgggttc gtctatcgaa cttttggatg
     9961 cggtcagcat cgccaaagaa aacggcgcgt ctgtcatcgc actgacccgc aacgattcgc
    10021 ctctggcgca acttgccgac tgcgtgttga gcgttgccac acaggaaaat gccgaactct
    10081 acacgcccat ggtttcccgc ctcttgcagc ttgccgtcat cgacattctc gccatcggac
    10141 ttgccctgcg cttgggcgat gctgccagcc tgcaattgca gaaaagcaaa aaaagcatac
    10201 acaacaagca catcgattac gacaaagatt gaccttcaga cggcatccca caaatgccgt
    10261 ctgaaatgcc gaacaacggt cgtcggcggc ttgcggcagt ttccggcagc cttttcatcc
    10321 cacaacaaaa aacctcattc aggagcatat agatgaaaca ccttcacgac ttacccgcat
    10381 ggtcgaaatt gtggaaccac tttgacgaca gcaaaacatt gcatatgcgc gaaatgttcg
    10441 agcaagaccc gcagcgtgcg gaacgctact ggctgcaagt cggcggactg acgctggact
    10501 actccaaaaa ccgtatcaac gacgaaacca tgtcgctttt gttcgagttg gcgtgtgaag
    10561 caggcgtgcc ggagcggatg cggcagatgt tccacggcga aaaaatcaat accaccgaaa
    10621 accgcgccgt cctgcatgtc gcccttcgca accgcaccaa ttcgccgatt gtggttgacg
    10681 gtgaagatgt gatgcccaaa gtcaaccgcg ttttgcaacg tatgggcgaa tttgcacacg
    10741 aagtccgcag cggaagctgg ctgggctata ccaaccaagt cattaccgac gttgtcaaca
    10801 tcggcatcgg cggatcggat ttgggcccgc tgatgatgtg taccgcgctc aaacctttcg
    10861 gtcatccgcg cctcaatatg cacttcgtct ccaacgtgga cggctcgcaa ctgcgcgacg
    10921 tattgtccaa agtccacccc gaaaccacgc tgttcatcat cgcctccaaa acatttacca
    10981 cgcaggaaac gctgaccaac gcgctgaccg cgcgcgaatg gtttttgaat catgcgggcg
    11041 acgaagaagc cgttgccaaa cacttcgccg ccgtttccac caatcaaaaa gccgtcgccg
    11101 aattcggcat cgacaccgcc aatatgtttg aattttggga ttgggtcggc ggtcggtaca
    11161 gcctgtggtc cgccatcgga ttgccgatta tgctgtatct cggcgaagaa aacttcattg
    11221 aaatgctcaa cggcgcgcac ctgatggacc aacacttcat caacacaccg ctcgagcgca
    11281 acctgcccgt cattctcgcc ctcatcggca tctggtatat caactactac ggcggcggca
    11341 gccacgtcat cgcgccttac gaccaacatt tgcaccgcct gcccaaattc atccagcagc
    11401 tcgatatgga aagtaacggc aaacaggtta cgttggacgg caaagcagtc ggacacgaaa
    11461 cctcgccgat tatctggggc gaaacgggca ttaacggcca gcacgccttt ttccaactgc
    11521 tgcaccaagg cacgcacatt acccccatcg acctgattgc ctcgcttgaa aaacgcagca
    11581 acctgcccgg acaccacgaa atcctgcttg ccaacgtctt cgcccaagca gaagccttta
    11641 tgcgcggcaa aacccccgac gaagtccgcg ccgaactcaa agcgcagggt atggatgagg
    11701 cgcgcatcga agagctggtc ccgcacaaaa ccttctccgg caaccgcccg accaacctca
    11761 ttctcatgga caaggtcaac ccgcgcaata tgggcagcct gattgccatg tacgaacaca
    11821 aaaccttcgt acaaggcatc atttggggca tcaacagctt cgaccagtgg ggcgtggaac
    11881 tcggcaaaca actggctaaa accattttgg gcgaactgac cggcgaaacc gggccgcaaa
    11941 agcacgacag ttcgaccgaa cgcctgatca acctctacct gcagaccaac cgcaaataaa
    12001 acctgcggaa aaatgccgtc tgaacgccga ccgtttcaaa cggcattttt atcgaacagg
    12061 aaaaccgtcg gtaaacttgc agagcgtgtg caatcccgat atgatggttt gcataaattt
    12121 aaacatatat gttccgcagc tatggcactg attaaagagc cgttggacaa agtgaaacaa
    12181 aggaacgaag aacttgaagc ggcagaagaa gcggcggcgc aggaggcatt gggtcgggag
    12241 caggaagccg cccgcgtatc cgaatgggaa gaacgctaca agctgtcgcg cagcgagttc
    12301 gagcagttct ggaaaggatt gcctcaaacc gtacagaata agctgcaagc ctcacagaaa
    12361 acatggaaaa gcgggatgga taaaatctgt gccaacaatg cgaaagctga aggtaaaacg
    12421 ccaaacggca taaaattcag cgaactggca tgcaaaacgg cgaaaaccga agcacgcttg
    12481 gaagagctgc acaaccgtaa aaaagccctt atcgacgaaa tggccaggga agcggacaag
    12541 aaagaactgt caaagcggct ctgaacagcg cggttcaggc actgcccgcc gatattgccg
    12601 aaaccgtcat gcccgagtac cgcaactgac aaaacggttt gaatgccaaa tgcgccgacg
    12661 gcgacgaata cggcgtggca caatctgact gccgtaccag agaaatcaat gcgaaaacca
    12721 aagaaatcca aggttatctg attgactgaa acttggatgc gggaatgtcg gcggcttttg
    12781 cgtttttgtc gtttttatag tgaattaaat ttaaaccgat acagcgttg
//
LOCUS       DAWFJW010000059        12826 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFJW010000059 DAWFJW010000000
VERSION     DAWFJW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12826
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.059"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..1257)
                     /gene="ispG"
                     /locus_tag="WGR19_001284"
                     /gene_synonym="gcpE"
     CDS             complement(<1..1257)
                     /gene="ispG"
                     /locus_tag="WGR19_001284"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0798814.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQ"
     gene            complement(1273..2034)
                     /gene="pilW"
                     /locus_tag="WGR19_001285"
     CDS             complement(1273..2034)
                     /gene="pilW"
                     /locus_tag="WGR19_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0798815.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(2037..3131)
                     /gene="rlmN"
                     /locus_tag="WGR19_001286"
     CDS             complement(2037..3131)
                     /gene="rlmN"
                     /locus_tag="WGR19_001286"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0798816.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(3274..3699)
                     /gene="ndk"
                     /locus_tag="WGR19_001287"
     CDS             complement(3274..3699)
                     /gene="ndk"
                     /locus_tag="WGR19_001287"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0798817.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(3758..4909)
                     /locus_tag="WGR19_001288"
     CDS             complement(3758..4909)
                     /locus_tag="WGR19_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0798818.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(5300..6127)
                     /gene="fghA"
                     /locus_tag="WGR19_001289"
     CDS             complement(5300..6127)
                     /gene="fghA"
                     /locus_tag="WGR19_001289"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0798819.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(6136..7245)
                     /locus_tag="WGR19_001290"
     CDS             complement(6136..7245)
                     /locus_tag="WGR19_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0798820.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            7393..7800
                     /locus_tag="WGR19_001291"
     CDS             7393..7800
                     /locus_tag="WGR19_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0798821.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(8151..8465)
                     /locus_tag="WGR19_001292"
     CDS             complement(8151..8465)
                     /locus_tag="WGR19_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0798822.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(8476..10161)
                     /gene="rpsA"
                     /locus_tag="WGR19_001293"
     CDS             complement(8476..10161)
                     /gene="rpsA"
                     /locus_tag="WGR19_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0798823.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(10317..10973)
                     /locus_tag="WGR19_001294"
     CDS             complement(10317..10973)
                     /locus_tag="WGR19_001294"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0798824.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRNFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(11217..12025)
                     /locus_tag="WGR19_001295"
                     /pseudo
     CDS             complement(11217..12025)
                     /locus_tag="WGR19_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(12085..12262)
                     /locus_tag="WGR19_001296"
                     /pseudo
     CDS             complement(12085..12262)
                     /locus_tag="WGR19_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348547.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            complement(12229..12591)
                     /locus_tag="WGR19_001297"
     CDS             complement(12229..12591)
                     /locus_tag="WGR19_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0798825.1"
                     /translation="MANKAKSVTDDDKLTHHQAFILTIAGATVGFGATWRFPYLVGEN
                     GGGAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWM
                     GCSARSASWLITWYSAAG"
BASE COUNT         3062 a         3082 c         3257 g         3425 t
ORIGIN      
        1 ctgtataggg atgattttgt tctggttgcg tttggagccg ttttcaccat agttggtttt
       61 gacatattcc tctacaatag ctaggaattc agttgcaata ttatcacctt tcagtgttat
      121 tttgcgctcc ccgtccacat aaaccggcgc gacgggtgtt tctcccgtac cgggaaggct
      181 gatgccgatg tcggccaatt tgctttctcc gggcccattg acaacgcagc ccattacggc
      241 aacgttcagg gattcaaccc caggataaag ggtacgccat atagacattt tttggcgcag
      301 gtaattttga acatcttgtg ccagctcttg aaatacggta ctggtggtac gcccgcagcc
      361 ggggcaggcg gtaaccatcg gcgtaaacga acgcaatccc atagtttgta aaatctcttg
      421 cccgacgacg acctcctgag tacgcgggct gccaggttcc ggagtcagtg aaatgcggat
      481 ggtgtcgccg attccttctt gaagcaagac ggataatgcc gccgttgatg cgacaatgcc
      541 tttgctgccc ataccggctt cggtcaaacc caaatgcagc ggataggcgc aacggctgcc
      601 cagttcgcgg taaacctgaa tcaaatcctg aaccgcgctg actttgcacg acaggatgat
      661 tttgtcttcg ggcagtccca atagaacggc tttttcggca gattccaaag cggagacaat
      721 cagtgcttcc ttcgtcactt cttcgggcgg tttcggcgcg gaagaagcga ggttggcatc
      781 catcatacgt ttggcgaggc tttgatccaa agaaccccag tttacgccga tgcggacggc
      841 tttatcgttt tcagcagcag tccgaatcat aaaggcaaat ttttcatcgc cttttacgcc
      901 tttgccgaca ttgccgggat tgatgcggta tttggacaat gctttgccgc attcgggaaa
      961 ttccgccaac aggcgttcgc cgttgaagtg gaaatcgccg ataagtggtg tggcatagcc
     1021 catatcgtcc aagcggcggc ggatttcggc aactttggac gcggcttcgg ggctgttgac
     1081 ggtaatacgc accatttcgg atccggcatc gctcaattcc ttaatctgca atgcggtggc
     1141 ttttgcatcg gcagtgtcgg tgttggtcat agattggata acgacgggtg cttctgaacc
     1201 gacggtaata tgatcgatgc ggacttgatg cgtcttgcgg cgttggagtg tgttcatatg
     1261 gtttgaatct gtttattgac cgatgaggac ggtttgcaat tcttccgagt aggggaaatt
     1321 cgcctgcaat tgtgcttcat attcgtatgc cgcctgtgcg ttgccgaggg ctttggcaat
     1381 tttccagcct agcagcaaat catcggcctg aaggacttct accctgcttt ggtatttttt
     1441 aaagtagtaa tcggcatcgc ccaactgccc ggccagcatt ttggtgcgcg ccagttcttt
     1501 aaatgcgggt gggaactgcg gctgggcggc gagggaacgt ttcaaatagg cttccgccaa
     1561 tccgaattgc ccctgttttg cgctgcatat gcctttattc aggttggcaa tataaggggt
     1621 cgggtaggtg gggtcggcca gggctttgtc gaaatatgcc atagattcgg cagggcggtt
     1681 gagcctgccg cacaggaacc agccgtagtt gttgttgatt tcggcactgt cgggtttgat
     1741 ggagagggct tgccggaaac tttcctgcgc cttgtcgtta actttcaggt attgatagat
     1801 ttcggcacgg accagccagg caagctcgtt tttagggtcc gatttcaggg cgtcttcaat
     1861 acttgccgtc gcctgacggt agtcctgacc gcgcatatat tccattgcca actgggtttt
     1921 gatattggaa acctgattgg ctttttctgc ccgcgagggg cggtaggaag tgctgcacgc
     1981 gcccaaggca aggacaagta ataaagagat tcgtttggat ggcttaaaag gcataattac
     2041 ccctgttgtc cgattaaaat ctgctgccat ttttgttggc ggcgcgtttt atcctgaacc
     2101 tgccccgcca actgtccgca ggcggcatcg atgtcgtcgc cgcgcgtttt tcgtacggta
     2161 acgacaaatc ctgcctgctg caaaatatcg cggaacacac ggatgttctc attgctggag
     2221 cgttcgtatc cagagtttgg aaagggattg aacggaatca gattgaactt gcagggaaca
     2281 tctttgacca gttcgatcag ttcgcgcgca tgttgcgcct tatcgtttat tccgtccaac
     2341 atgacgtatt cgaaagtgat gaaatccctg ggtgctttga ctagatagcg ttggcatgca
     2401 gccatcaatt ctttcaaggg atattttttg ttcaacggta cgatttggtt gcggacttcg
     2461 tcattggaag cgtggaggga aaccgccaaa gccaccggca tgacatcgcg caacctgtcc
     2521 atttggggaa ccatacccga agtggaaacg gttacgcggc ggcggctcaa accgtagccg
     2581 tggtcgtcca gcatgatgct taaggcggta acgacattgt cgaagttcgc catcggctcg
     2641 cccataccca tcatcactac attggaaatt acgcgttcgt ttttcggtgt aacacccatc
     2701 gctttgtttg cccaccacaa ttgcccgatg atttcggcag cagtcaaatt gcggttgaag
     2761 ccctgccggc cggtcgaaca aaatgtacat tccaaagcgc agccgacttg tgaggaaatg
     2821 cagagcgtgc cgcgatccga ttcggggatg aagacggttt ccaccccgtt gcccgtaccg
     2881 acatccaaaa gccattttcg agtgccgtct gaagattttt gagacatcat cagcttggga
     2941 atttcgatgc ctgcctgttc gttcagttta tggcgcaacg attttgccaa atcggtcatt
     3001 tcgttaaaat tttgcgcgcc ggattggtgc atccaacgca taacctgttt ggcacggaag
     3061 ggtttttcgc ccatatcggc aaaatgtcgg gtcagccctt gaaggtcgta gttgagcaga
     3121 ttggttttca tgtgtttgtg tttttcttaa attaaggctt caaataaaaa attgcagggc
     3181 aggcaagttt gatttgaagc cttttgcgac agagttttca gacggcatgg atatatttat
     3241 gccgtctgaa gaggagttgt gcggtgtatt gtatcaacgg gggcagattt cggtttggct
     3301 gaaaaagtag gcaatttcca aagcggcatt ttccacgctg tcggaaccgt gtacggcatt
     3361 aatgctgacc gaagtggcaa agtccgcgcg tatcgtgcct tcggcggctt cggaaggatt
     3421 agttgcaccc atcagttcgc ggtttttcag gacggcgttt tcaccctcta atacctgaat
     3481 cataaccgga ccgccggtca taaattcaac caatccggcg tagaaggggc ggtctttatg
     3541 aaccgcataa aattcttgcg cctctttgag agtaagctgc ttcattttgg cggcaacgat
     3601 tttcagaccg ttctcctcaa agcggctgta tattttgccg ataacatttt tgccgacggc
     3661 atcgggtttg atgatggaga tggtacgttc aatcgccatg ctatatcctt atttttactt
     3721 aagaagaatc aaatcggtat tctatcaaaa aaataactta tccgcctgaa gccttgggag
     3781 atagggtcag gtgcggctgt tccaaataaa cttcggactg catttcgacc atccggctgg
     3841 cggtgcgggt aaattcttcg gcaaaatcgc cttccgtata gatatggttg acatctaccg
     3901 cgccggtggc acacagtttg acccggaaat cgtagagtac gtcaatcagc caagtcagcc
     3961 gccgcgcctc cgccttttct tgcggtgaga gttgttccaa acctgaaata aaaaccattt
     4021 cataatgttc ggccaaatac agatagtcgg actgtgagcg ggggccgaag cacagtgcgc
     4081 ggaaatcaaa ccatatggca cggccggact cggctttgtg gggaatctcc cgaccatgga
     4141 tggtgctgat gccggggttc aaatcggtaa tgcctgtcat ttctttgaac agttttgcca
     4201 gttttgcctc attttcttca ttggcaggcg taaagaaaat ctcggcgggg cggagggtac
     4261 gcagtcggta gtcttcaccg ccgtcaacgt ttaagacggt caggctggac tcgatgagcg
     4321 cgattgtggg aagaaaactg ctccggtttt gaccttgcgg gtagagttcg gaaggcgcgt
     4381 agtttgaagt cgccaccaaa acaacgccct cgttaagcag gttttccagc agacggccta
     4441 aaatcattgc atccgcaata tcgctgacat gaaattcgtc aaaacataat acgcgggttt
     4501 ctttggcaat ctcggcggca acggatttca acgggttgct ttcgcttttc agggttttca
     4561 gcctctggtg gatttctgcc ataaaggcat gaaagtggac gcggcgtttg cggcggtaag
     4621 ggaggcagcc gaaaaaagcg tccatcagaa agcttttgcc gcgtccgacc ccgccataga
     4681 aataaagccc tttggggact tgcggggaac gcaaactcct gcctaaaaaa cggtttcttt
     4741 tgcgtttgaa catcatcaat tcggtccaaa gccgatcgag gtgttcgatg gcggctgcct
     4801 gcgcgtcgtc gcggatgaag ttgggcagtt gtgaggcagc ctgataccag gtcagcgggc
     4861 tgtggttttc aaacggcggg gctttaaaaa gttggtctct attactcata tttaaccttg
     4921 ttatacttct tgcggcggag aaagaaaatg cttaccgtcg gtattataaa tgatttcctt
     4981 caaacttggc attttccgtg gaaaagaata atgacgcgca caagggggta taagcaaagg
     5041 cggcgtttca atatggtgca gatatgagct caaatgcctt ttatagtaga ttaaatttaa
     5101 accagtacag cgttgcctcg ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct
     5161 tgtcctgatt tttgttaatc cactataatc cgtagagtcg gctccttccg gctcagacgg
     5221 catacgttta tgccggcact tcttaccgcc ctcgtgccgg ctatcccaaa actgctttga
     5281 acactgaacg ctctttggtt tacttcaaaa acgcggcgtg ataagcaata tgctcgccaa
     5341 taaaactggc gatgaagtaa tagctgtgat cgtagccttt atggaaacgc acatcgaccg
     5401 gctggtttgc cgcacggcag gtttcgataa aatcttcggt acgcaattgt gtcggcaaaa
     5461 actcatcttc caagccttga tcgatgcgca taccttgcac tttatagcct tgttgaatga
     5521 gtgagttcgc atcatattgc tgccattttt cacggtcttt ccctaaataa gcagtaaagg
     5581 ctttttctcc ccacggcacg aggcttggcg ataaaatagg cgaaaaggca gaaacacttt
     5641 gataacgttc ctgattccgc agcgccaata ccaatgcgcc gtgtccgccc attgaatgcc
     5701 ccataatgga acgtttgccg ttggtaggaa agtgtttctc aatcagacgg ggcagctcgt
     5761 tcaaaatgta atcatacatt tgataattcg ccgcccaagg ctgttcggtc gcattcaaat
     5821 aaaagcctgc accctgtcct aaatcgtaag catcatcgtt cggcacttgc tctccgcgag
     5881 ggctggtatc ggaggccacc acaattactt gatgttctgc cgcataacgc tgaaagcctg
     5941 atttggtaat gaaattttgt tccgtacacg tcaagccgga aagccaataa atcacaccaa
     6001 gcggtcgatt ttctggatta tttggcaaat agacggcaaa tttcatttcg cattgcagcg
     6061 tttgggcatg atgcgcccaa acttgttgcg aaccgccaaa aatttgatgt tgttcaatca
     6121 gtttcatcgc ataccttagt agtgaataac ggcgcggatc gatttacctt cgtgcattaa
     6181 gtcaaaggct ttattgattt gatcgagtgt cattgtgtgg gttacaaacg gttctaactc
     6241 aatgtcgcct ttcattgaat cttccaccat tttcggcagt tcagagcgac ctttcacacc
     6301 gccaaatgct gaacctttcc aaacacgacc tgttaccaac tggaacggac gcgttgaaat
     6361 ttcttgtcct gcacctgcta cgccgatgat aatggattga ccccaaccac gatgtgcact
     6421 ttctaatgcc tgacgcatta cgtttacgtt gccgatacat tcaaaggtat ggtcaatgcc
     6481 ccatttatta atgtctaaca acacatcttt gatcggttta tcgtaatcgt tcgggttcaa
     6541 acaatccgtt gcaccaaact gttttgccaa ctcaaatttt gatggattgg tatcaatggc
     6601 gataatgcgg ccggctttgg cttgacgcgc accttgcacc accgccaaac caatcgcgcc
     6661 taagccaaac acggcaacag agtcgccttc ttgcactttt gccgtattat gtaccgcacc
     6721 aatacctgtg gtaacgccgc agccgagcaa acatacttgt tcatggttgg cttcagggtt
     6781 gattttcgcc agtgaaactt cggcaacaac ggagtattca ctgaaagtcg aacagcccat
     6841 atagtgataa atcggctgac cttgataaga aaaacgcgtc gtgccgtccg gcattaagcc
     6901 tttaccttgt gtatcacgca ctgagacgca caagttggtt ttacctgaac aacaaaactc
     6961 acattcgcca cattcggcgg tgtaaagcgg aatcacgtga tcacccggtt ttacgcttga
     7021 cacaccttcg cccacagcaa cgaccacacc cgcaccttcg tgtccaagca ccacagggaa
     7081 tacgccttca ggatcgcttc ctgataacgt aaacgcatca gtatggcaca cgccagtgtg
     7141 ggtattgcgg attaacacct cgcctttacg cggcatttct acgtcgattt ccacaatttg
     7201 taagggttgg ttaggggcga atgccaccgc cgcacgagat ttgatggttg aatcggcttg
     7261 tttcatttcc attgtctttc ctcttaattc attgacaaag ttgccttgcc tgagcaacat
     7321 agcaacagtt tacactttag agttaactct aaagcaagtg ttttttactg tttttgttta
     7381 gaaggaggga aaatgactta tactactgcc aaagccgccg aaaaaatagg catctccgcc
     7441 cacaccctac gtttttacga caaagaaggt ttgttgccca atatcggacg tgatgaatac
     7501 ggtaaccgct gttttaccga taacgatttg caatggttgg gcttattgca atgcttgaaa
     7561 aatacgggaa tgagcttaaa agacatcaaa cgctttgcgg aatgtaccgt cattggcgac
     7621 gataccattg aagaacgcct ttccttgttt gaaaatcaaa tagaaaatgt gaagtgtcaa
     7681 attgccgaat taaaacgcta tttagatttg cttgaataca aattggcgtt ttaccaaaaa
     7741 gcgaaagcat taggctcggt aaaagctgta aatttgccgc aaattcctga aacggcttag
     7801 ttttagtgca ataagcagga tttttgtcct gttcgtaata gaaatcaaaa atttgttgtt
     7861 taaaaagcgg attgtaagca atgatatagt ggattaacaa aaatcaggac aaggcgacga
     7921 agccgcagac agtgtaaata gtacggaacc gattcacttg gtgcttcagc accttagaga
     7981 attgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa tccactataa
     8041 aaaaatccac gctctaatcc gttgcttgct tggctcaagg tattgaagca acggatgaag
     8101 ttaaaccaca gaaaaaacgc cgcgtaacag cggcgttttg atgctaaggt ttaattggca
     8161 ttttctttta aagccaagtc gacccgctcg cgcaattctt taccgggctt gaagtggggt
     8221 acatgttttt caggtacttc cacacgctcg ccggttttgg gattgcgacc gatgcgggca
     8281 ggacgatggt tcaaatcgaa gctgccgaaa ccgcggattt cgatgcgttg acctcgggca
     8341 agcgatctag tcatggtgtc aaccaagact tttacgctgt actctacgtc ttttgccaga
     8401 agatgcgtgc cgtttttggc ggcaaacact tctgccaaac gaaccattaa ctcagacttt
     8461 gtcatgtctg caaccttatt cttgttcgcc ggagagtttg gctttcagca ggtcgcccaa
     8521 gctggtggtg ccggcattcg cattggcggc ggcattgacg gagttcagtg cttcgcggct
     8581 ttctttggca tctttggctt taacggaaag tttgatgctg cggtttttgc ggtcaacggt
     8641 aacgatgacg gcttcaactt cgtcgccttc tttcagtttg gtggtcaaat cttcaacgcg
     8701 gtcggctgca aattcggaag caggcaggta gccttctact tcgtcagaca gggcgataac
     8761 agcacctttg gcgtcaacag atttcacgga acctttaacc aaagaacctt tgtcgttcac
     8821 gctgatgaag ttgccgaacg gatcgccttc cagttgtttg atacccaagg agatgcgttc
     8881 tttttccacg tcgattgcca atacgacggc ttcgacttct tcgccttttt tgtatttgcg
     8941 tacggcttct tcgccggatt cggtccagga caggtcggac aggtgaacca aaccgtcgat
     9001 gccgccgggc agaccgacga atacgccgaa atcggtaatg gatttaaccg cgccggagat
     9061 tttgtcgcct ttgttgtggt tggcggcaaa ttcttcccaa ggattggctt ggcattgttt
     9121 catacccaaa gagatacgcc ggcggctttc atcgatttcg aggatcatta cttcgacttc
     9181 gtcgcccaat tgtacaactt tgctcgggtg tacatttttg ttggtccaat ccatttcgga
     9241 aacgtgcacc aagccttcga tgccttgttc gatttcaacg aacgcaccgt agtcggtcag
     9301 gttggatact ttaccgaaca ggcgggtacc ttgcgggtaa cgacgggtca ggccgctcca
     9361 aggatcttcg cccagttgtt tcatacccag agaaacgcgt tgtttgtctt ggtcgaattt
     9421 caggacttta gcttctactt cttgaccgac ttccagaact tcgctcgggt gtttcacacg
     9481 gcgccatgcc aagtcggtga tgtgcagcag accatcgatg ccgcccaagt caacgaatgc
     9541 accgtaatcg gtaatgtttt taacgatgcc tttgatgacg gagccttctt gcaggttttc
     9601 cagcagggct ttgcgttctt cacccaaggt agcttccaga acggcgcggc gggacacaac
     9661 aacgttgttg cgttttttgt ccagtttgat cactttgaat tcaatttctt taccttcgaa
     9721 gtgagaagtg tcttttacag gacgtacgtc gaccaaagaa cccggcagga atgcgcggat
     9781 gctgctaatc ataacggtca ggccgccttt gactttgccg ttgatgatgc cggacaggat
     9841 gttgccgttt tccatggcct cttccagggc aatccaatcg gcagcgcgtt tggctttttc
     9901 gcgggacagt ttggtttcgc cgaagccgtt ttcgacggat tcgatggtaa cggtaacgaa
     9961 gtcgccgact ttaacttcaa tttcgccttg agcgtttttg aattcagcta catcaatcag
    10021 ggattctgat ttcagacctg cgtttacggt aacgaagttt tggtcgattg ccactacttc
    10081 agcggtaatc acctcacccg ggttcatttc ttgcagggta aagctttctt ccaacagctg
    10141 agcaaaattt tccatagaca tatataactc ttttcggtac accgccaagg ggtgcggggt
    10201 gggttggtgg atatctgccg tccttggcag ggcagacggg tacataaggt ttcagacggc
    10261 atatggggga tttttatgcc gtctgaaacg taatttgtgc gattatacct gaaaatttaa
    10321 acttcacgat accaatcaag tacttttttt acagtttctt ctatagtcag gcggcttgtg
    10381 tccaaaagca gggcatcggg ctgttgtttc aggggggcaa ctttgcggtt tcggtctgcc
    10441 tcgtctctgg cttcgatgtc ggacaggatg cgctcgaatg ccaaaccttc gcaggggatg
    10501 ccgatttgtt tggcgcggcg ttcggcacgg attttggatt ctgccgtcag gaagatttta
    10561 agttcggctt gggggaagac gaccgatccg gtgtcccgtc cgtcggcaac cagtcctttt
    10621 tcggtcagaa aattgcgttg gcgttgcagc agggcggcgc ggactttagg caactgtgcg
    10681 actgcggatg cgcccatgcc gatggcttct gtccggatgc cgtctgaaac gtcttcgccg
    10741 ccgagcagga tgcggctgcc tgaaaatacg gcgggcagtt tttttgccag ttcggaaacg
    10801 ttttcttcat cgtgccatcc cacgccttgt ttttgtgcat atagggcagt caggcggtag
    10861 agtgcgccgg tatcgagata atcgtatccc aatgcggcgg caacgcgggc ggcaaccgtg
    10921 cctttgcccg atgcgcccgg gccgtcgatg gcgatgactt tttgtctgtt cataaggggg
    10981 attcctgatg gtttggggta tgggttttgc cgtctgaagg atgtgtttcc cgttggggcg
    11041 gattctacct gttttaaagg acgattgtct aagcagacgg aacgccgcct gcccgaaaac
    11101 atccgacaga agccggcaag ccggttcgga ttccggcgtt gcgggtattg tggcgggcat
    11161 caaggcaatg ccgtctgaaa gaggtatggc cttcagacgg catcgattgc tgcagattag
    11221 aacaggttgc tgacaaaaat cagcaaaatc accaacggca caagatattt cacataagca
    11281 aaccaaatat tgaccgtcgt atggttgcct ttgtaaagca attcttcctt cgcttcgtcc
    11341 ttcatcacaa aaccgacaaa cagcgcgaaa ccgagcgcgg tcagcataaa caagatgttg
    11401 ccgctgatgt agtcgaaggc atcgaaaata tttttgccga acacggaacg tctttccacg
    11461 aaccatagct cagaatggac gggatgttgc cgaaaatgaa gatggcggct aatacaatcg
    11521 taatcgcggc ggtacggcgg attttggttt tttcctgaat ggtcgtaatc aacacttcat
    11581 aaatggtcag cgaagtcgtc aatgcggcaa tcaggagcag cgagaagaaa atcacggcga
    11641 acacagatct cgcccacata tgtgagaaca caatcggcaa gctttggaac accaaagtcg
    11701 ggccggaatc gggggcaacg ccgaagctga agagcgacgg gaaaatcata aagcccgcaa
    11761 gtatggcgat gatggtattg gtaattgccg tgataactgc cgtctgaacc agattttcgt
    11821 ttttatccaa atagctggac aaggtaatca tcacgccgaa acccaggctc agggcaaaaa
    11881 atacctgccc caaaacgaag acgaacagtt cggcggtaat cttgctgaaa tcaggtttca
    11941 gatagaaagc aaccccttcc attgcgcccg gaagggtaac gttgcggacg accatcgcga
    12001 ttaggaacaa aaacagcagc ggcatcagct attttgccgc tttttcaatg ccgccgatca
    12061 cgcctttgac caaaatccat tggttcacgg cgacaaaaag cagcgtataa aacgcaattt
    12121 cccaagggct gttttcgatg tgttcggtaa agaagctttt tgtaaccata ccgtcgacgg
    12181 ggctggaaat gtccagattg ccgccgataa tgttgacgat atagctgatt acccagccgc
    12241 cgagtaccat gtaataagcc atgatgccga acgcgccgag cagcccatcc agccgaccag
    12301 tttccaaatt ttggcaatgg gtttgccgtt catcgggccg ccgaacgcat ccagcgcgtt
    12361 cacgcctttg cgccgtccga tgacattttc caccaaaatc atcgggatgc cgataaccag
    12421 catcgcgata cagaataaaa acacatacgc gccaccaccg ttttcaccga ccaaatacgg
    12481 gaaacgccac gtcgcgccga aaccgacagt cgcgccggca atggtcagga taaaggcttg
    12541 atggtgggta agtttgtcat catcggtgac ggatttggct ttgttggcga tgaccaagcc
    12601 caaatggtgc tggcgagata ggggctgtaa ggtgggggtg tcgtttcatg ggggttcctg
    12661 ttggtaaatt aaattttatt ataaaataaa aaaaccacaa acactagggc gatgtttgtg
    12721 gtattgtggc acataatgct gatcaataat ttaaggtata acttatccca taacttctcc
    12781 cctgtgcaga caagctggat aattcaccat aagtcacttt ggcagc
//
LOCUS       DAWFJW010000060        12812 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFJW010000060 DAWFJW010000000
VERSION     DAWFJW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.060"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(617..1387)
                     /gene="xth"
                     /locus_tag="WGR19_001298"
     CDS             complement(617..1387)
                     /gene="xth"
                     /locus_tag="WGR19_001298"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0798826.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1441..1728)
                     /locus_tag="WGR19_001299"
     CDS             complement(1441..1728)
                     /locus_tag="WGR19_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0798827.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            1830..2063
                     /locus_tag="WGR19_001300"
     CDS             1830..2063
                     /locus_tag="WGR19_001300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798828.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2196..3077)
                     /gene="nadC"
                     /locus_tag="WGR19_001301"
     CDS             complement(2196..3077)
                     /gene="nadC"
                     /locus_tag="WGR19_001301"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0798829.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3153..4091)
                     /locus_tag="WGR19_001302"
     CDS             complement(3153..4091)
                     /locus_tag="WGR19_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798830.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            complement(4219..4356)
                     /locus_tag="WGR19_001303"
     CDS             complement(4219..4356)
                     /locus_tag="WGR19_001303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0798831.1"
                     /translation="MPSAFQTVLYYAQLEYNKQGRLKTGLRYRKTDPLRSDKPLWFIV
                     D"
     gene            4396..5508
                     /gene="nadA"
                     /locus_tag="WGR19_001304"
     CDS             4396..5508
                     /gene="nadA"
                     /locus_tag="WGR19_001304"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0798832.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNSGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            5560..7068
                     /gene="nadB"
                     /locus_tag="WGR19_001305"
     CDS             5560..7068
                     /gene="nadB"
                     /locus_tag="WGR19_001305"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0798833.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKLAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLTVQIRCRPNIRVCEQQMALDIQTES
                     GAACGLTVLDCRTQATYSIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(7232..7897)
                     /gene="pgmB"
                     /locus_tag="WGR19_001306"
     CDS             complement(7232..7897)
                     /gene="pgmB"
                     /locus_tag="WGR19_001306"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0798834.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGVLPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPVGVGKAEDLGSDIALVSGTAGLTYAYLQNVWTQ
                     SGR"
     gene            complement(7909..10167)
                     /locus_tag="WGR19_001307"
     CDS             complement(7909..10167)
                     /locus_tag="WGR19_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0798835.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSIDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDDRSYIATQTVTNPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEAEVGGTPETFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKIAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRRGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNMDEKRRNFDFYEPMTVHESSLSPC
                     IHAILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLELQVYGKDI
                     TLNGSHTVTLEK"
     gene            complement(10400..11419)
                     /locus_tag="WGR19_001308"
     CDS             complement(10400..11419)
                     /locus_tag="WGR19_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0798836.1"
                     /translation="MSDTPATPATRDFGLIDGRAVTSYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYG
                     IESLS"
     gene            complement(11423..12778)
                     /locus_tag="WGR19_001309"
     CDS             complement(11423..12778)
                     /locus_tag="WGR19_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0798837.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
BASE COUNT         2797 a         3548 c         3609 g         2858 t
ORIGIN      
        1 aacatcgcta gagatgggcg actttgattt tccatttgtt gattagtaaa ggatataaat
       61 actaacttgc cattgtcaaa ccacatagat ttatatagtg gattaaattt aaaccagtac
      121 gtcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
      181 tttttgttaa tccactatag ttattgcctt tgggagaatc aattctgaca gcacataaca
      241 taggttcatc tttacagtcg ttatattggg tttcatcaaa ctcaactgca tagcacaata
      301 aaggcaatag gaaaaaaagg agattaattt attactatac ataaggcagc ctgattattc
      361 attctaaaat aaatccggtt ttcaatattt gattttattc cggattatat acacaataaa
      421 ttttaccgct ccaaaatctg atattaaccc tatggatttc ataaattttt cagaaaaata
      481 ggctgtgctt gatcaatccg ccgactccgg ctgtttgtca gggaaaaggc aattattttc
      541 ggttagtcag cagcctatcc tattgaatca cttaaagata tggatgtttg tcaacgccat
      601 atcaaaacac ggtcttttac caatcgaatt ctgccgtcac cggcgcgtgg tcgctcggac
      661 gctccagcgc gcgcgtctcc aaatcaacgc ggacatcctt caacgccgcc gccatcgcag
      721 gcgacaccaa aatatggtcg atacgcaggc ccagtttgcg ttggaacatc gcgccgcgat
      781 agtcgaacca ggtatagaac gcgccttcgg gatggacttg gcgcaggctg tcggtcagtc
      841 ccaaatccag caggtttttg aaccactgcc gttcgacgga cgaacagtgg attttttcgt
      901 gccatttttc agggtcgtaa cagtccgcat cggcaggcgc gatattgaaa tcgcccagta
      961 gcaccagttt gccgtagcgg gtcatttcat cgcggacaaa ttcggttaac gcggcaaacc
     1021 attgttcttt atatttgaat ttcgggctgt cgagggcttc gccgttgacg caatagacat
     1081 tgatgacgcg cacaccgccg acggttgccg caatcacgcg ccgttgcgga tcgtccggca
     1141 gcgcgggcag cccgatatgc acgtcttccg gcgcgttgcg gctgacgatt gccacgccgt
     1201 tgtaggtttt ctgcccgctc caaacacagt gccagcccat catttgcaaa gcggcggccg
     1261 gaaatttgtc ctgatcgagc ttgagttcct gcaaaaccaa aatatcaggc ggattgtcgg
     1321 caagcaggtt ttgcacctgc ggcagccgca cattgagcga attgacgttc caagtggtga
     1381 ttttcataac atttccgaaa aatgccgtct gaaacgttca gacggcaaaa ggaaaggatt
     1441 ttaacaccat accgcgcgtt tgttttccaa atcgcggatt gtccgcagaa tcgttgccgc
     1501 gtcttcggaa atcaggcggt attcgataat gcggtggtaa cgcgtaaagt ccaccagacc
     1561 gcccgccctc aggcggttca aatggccgga aacggcggtg gccggcaggg ataaggattt
     1621 tgccagttcg gcgatattgc gttcgctgtc caacagttgg atcagtatcg ccatacgttc
     1681 gggatgcgcc ataagtttga gtatggtgtg gagcggtatt gtgttcatcg gtattcctaa
     1741 aacctttgcc gcccgccaag cggattgacg ggcgacagat atggatgatt gtgattatct
     1801 ttataaatcg tattataaat caaatattta tggatgattg tacaggattg tatgttcctt
     1861 ttcccacttt ttttgcaaac acgcgtaaag gcgggctttc agacggcata aaactgccgc
     1921 ccctaccctg cctcaccctg ttgccgaacg gaatcatccg cattgttcaa aagatgcctt
     1981 acaaccgccg cacgtcttgc cctgccgatg cctccgtgcc ttcgggcaaa caaaatacct
     2041 gcctgaaacg ctgttttttc taaagcatca cgaccttcac gaaaaatgcc gtctgaagaa
     2101 tatttcagac ggcatttcct ccttgaggca ataaccggat tacggttagg cggttccttg
     2161 tctgacatat cagacaactg ccgacaccct aaaactcatg ccaagaaatc caaacctatg
     2221 tccaacgagc ggctgctgtg ggtcagatag ccgagggcga tgccgtccac gccggtttgc
     2281 gccacgcgct tcaggcggtc gaagccgatg ccgcccgatg cttcgcaata gacggtgtgg
     2341 gggtgggcgg tttgcgtgtg gcagcggttt gccgcttctt tcagggtttc gtcgtccatg
     2401 ttgtccagca aaatccgttc cgcgcccgct gcgatggctt cgtccagttg tgccaacgtg
     2461 tccacttcga tttccacgca ggtcaatgct ccgaccgcct gttttgcctg ccgaacggct
     2521 tgggcgatgc tgccgcaata ggcgaggtgg ttgtctttga tgagcacggc gtcgtccaag
     2581 cccatgcggt ggttcacgcc gccgcctgcc ctgacggcgt atttttgcag gacgcgcagc
     2641 agggggatgg ttttgcggct gcacacgatg tctgtaccgt attcggcgac ttcggcaacg
     2701 gcacgcgcgg tggcggtggc gatgccgctt aagtgcgtga ggtagttgag tgcggtgcgt
     2761 tcggcggcga gcagcgcgca ggcgttgcct tcgacgacgg ccagcgtctg acctgcgcgg
     2821 acggcttgtc cgtcttggat ttcggcttgg aaacggacgg acggatccat cgtctgaaag
     2881 gcgagacacg ccaagtccat accggcgata acaccgtctt cgcggctgac gaggaagagt
     2941 ttggcggttt tgtcgggggc gataacggcg gcggacgtaa tgtcgccgcg cctgcccaag
     3001 tcttcgctca aggcttgttc gactatgggg cgcaacaggg tgtcgggcag ggaaaagagg
     3061 gtgttttcag acggcatgag gtttcctttg tcgaaaaaga ttgatgttgc accgatgggg
     3121 caaaacttca tttgatgccg tctgaaaacg ggctaacggc tgcccagcgg cagtccgatg
     3181 tccgaaatca gcctgtcggg caggacgtcg tcgcggaagc ggcaaagctg cgcgggacgg
     3241 cctttgctgc ccgatacgcc ggtatccgac ggctcgatga ggttttgctg ctgaatctgg
     3301 cggcggaagt tttgcttgtg cagcaatctt ccgctgatgg cttcgacact gttttgcagt
     3361 tgcagcagcg tgaattcggg cggcatcagc tcaaaaatca cggggcggta tttgattttg
     3421 gcgcgcaggc gggacagggc ggtcgccaat acgcggcggt ggtcgtggcg catgggctgc
     3481 cctgtgagcg cgaaatcgaa gtttgcctgc ggctcggcgg cttccactat caggccgctt
     3541 tcatacagca tttcatagcg ttgcaaaacg tattcttccg accagttttc cggttcgacc
     3601 ccccaacaca aatgaatgcg cttgagccgc atttgacgca cttcctccgt gtccgccgag
     3661 tttgcccaaa tgcgcagtcg gctgacgacg gcgtcgcgct gtccgccgtc ggtgcgcaag
     3721 tcttcccacg ggaaatagcc gtagcagtcc tgccatttcg catcgggatg caggatgctg
     3781 tcggctgcct cgcgcaccag ccccaaatag ctgacgtaca agacgggcat gccgtgttcg
     3841 ttgcggcggt gggtatcgac aaaggtgtaa agctgttcca catagcccat aggctgcgaa
     3901 gtctgcttgg caacccacag cttcacgccc gcctgcaagg aattgcgcag aggggagagc
     3961 gggccgttgg gcaggagtgt gccttgggcg acggttaata cccgcaggcc gccgtcggta
     4021 acggcaatca atacgggaac cagctcgacg atgctttgcg gcggggcttc ggcttcgggg
     4081 taggcatcca tgctcggctt tctgcgggtg ggaaaggctt tattgtatag tggattaaat
     4141 ttaaaccagt acagcgttgc ctcgccttgc cgtactattt gtactgtctg cggcttcgtc
     4201 gccttgtcct gatttttgtt aatccactat aaaccaaagc ggtttgtcgg aacgaagcgg
     4261 gtcggtttta cggtatcgca aacctgtttt cagacgacct tgcttattat actcaagttg
     4321 agcataatat aaaacagttt gaaaagcaga cggcaaacct tcatgccgtc tgaaaaccag
     4381 ctacaaggaa acatcatgca aaccgccgcc cgccgctcgt tcgactacga tatgcccctc
     4441 atccaaacgc cgacttccgc ctgccaaatc cgtcaggcgt gggcgaaggt tgccgatacg
     4501 cccgaccacg agacggcagg ccgtctgaaa gacgaaatca aggctttgct gaaggagaaa
     4561 aacgcggtct tggtggcgca ttattacgtc gatccgctga ttcaggattt ggctttggaa
     4621 acgggcggat gcgtgggcga ttcgctggaa atggcgcgct tcggtgcgga acacgaagcc
     4681 ggtacgctgg tggtggctgg tgtgcgcttc atgggcgaga gcgcgaaaat cctctgccct
     4741 gaaaaaacgg tgctgatgcc tgatttggag gcggaatgtt ctttggattt gggttgtccc
     4801 gaagaagcgt tttcagcgtt ttgcgaccaa caccccgacc gcacggtggt ggtgtacgcc
     4861 aacacttccg ccgccgtgaa agcgcgtgcc gactgggtgg taacgtcttc ggtggcgttg
     4921 gaaatcgtgt cgtatttgaa atcgcgcggc gagaaactga tttggggacc cgaccgccac
     4981 ctcggcgact acatccgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc
     5041 atcgtccata acgaattcaa agggcaagag ctggcggcgt tgaaggcgga acatcccgac
     5101 gcggtggtgc tggttcatcc cgaatcgccg caaagcgtca tcgaactggg cgacgtggtc
     5161 ggctcgacca gcaaactgct caaagccgcc gtatcgcgtc ctgaaaaaaa attcatcgtg
     5221 gcgaccgatt taggcatcct gcacgaaatg caaaagcagg cgcccgacaa agaatttatc
     5281 gccgcaccga cggcgggcaa cagcggaagc tgcaaaagct gcgcgttctg cccgtggatg
     5341 gcgatgaatt cgctgggcgg catcaaatac gccctgacaa gcgggcacaa cgaaatcctg
     5401 ttggacagaa agctgggcga agccgccaaa ctgcctttac agcgtatgct cgacttcgcg
     5461 gcaggactca aacgcgggga tgtgttcaac ggcatgggcc ccgcctgatt tgccgtcaat
     5521 attgccgttt cagacgacct ctcaaacaag gacaacacca tgcaaaccga ttgcgacgta
     5581 ttgattgccg gaaacgggct ggcggcactg acgctcgccc tgtcgctgcc tgaatcgttc
     5641 cgcatcgtca ttttgtgcaa aaaccggctg gacgacactg ccagccgtca tgcgcaaggc
     5701 gggattgcgg cggcgtggtc gggagaggac gacatcgaaa aacacgttgc cgataccttg
     5761 gaagcgggcg cgggtttgtg cgacgaagcc gccgtccgca ccatcctgtc gcagggcaaa
     5821 ctggcaatcg aatggctgct ggcgcagggc gtggcgttcg accaaaataa taacggcctg
     5881 cacctgacgc gcgaaggcgg gcacacctgc cgccgaatcg cccacgtcgc cgactacacg
     5941 ggcgaagccg tcatgcagag cctgactgtc caaatacgct gccgcccgaa catccgcgtt
     6001 tgcgagcagc agatggcgtt ggacatccaa accgaatcag gtgcggcgtg cggactgacc
     6061 gtcctcgact gccgaacgca agcaacctac agcatccgcg cccgccatac cgtactcgca
     6121 ggcggcggct tgggacagat ttacgccgcc accaccacgc cgcccgaatg cacgggcgac
     6181 gccatcgcca tggcgatacg cgcaggctgc gcagtcgaaa acctcgaatt tatccaattc
     6241 caccccacag gcttggcaag atcgtctgaa aacggccgca ccttcctgat ttccgaagcc
     6301 gtgcgcggcg aaggcggcat cctgaccaac caagcgggcg aacggtttat gccgtattac
     6361 gaccgccgcg ccgaactcgc gccgcgcgac atcgttgccc gcgccatcgc cgccgaaatc
     6421 gccaaacaaa cgcaagactt cgtctcactc gatatcagcc atcaacccgc agcgttcgtc
     6481 cgccggcatt tcccgtccat ccatcggcac tgtttatccc aatgcggcct ggatatcacg
     6541 cgccaagcca tccccgtccg ccccgtgcaa cactacacct gcggcggcat ccaaaccgac
     6601 ccctgcggca gaacttccct gccgcagctc tactccttag gcgaaaccgc ctgcaccggc
     6661 ttgcacggag ccaaccgcct tgccagcaac tccctgctcg aatgtgtcgt taccgccaga
     6721 cttgccgccc aaaccatcgc agacggacaa gcattccaag ccaaaacgtc tccaaggccg
     6781 tctgaaagcc tctcctccga agcaggcatc ttttcagacg gcatccaaag cgcattcagc
     6841 cgccccgtcc tgcaagcgtt caaccaacgc catctgggca tcctccgcaa cgataccggt
     6901 ctgcgctgcg ccatcgccca gctgcggctt tggaaacaaa accaaaccga accgcacacc
     6961 gcgtccgaat acgaaaaccg caacctgctc gaatgcagcc tcgccgtcgc ccaagccgca
     7021 tacaggcgga gacagaacat cggcgcgcat tttaatagtg attgttaagg ttggctggtt
     7081 agaaaaaaat cgttcaaata aatttgaata gcatggttga gaaattggca ttcagttttt
     7141 agggttgcgt aagttgggtt attattttgt tctaaaagta gaaaggccgt ctgaaatatt
     7201 tttcagacgg cctgtttact tcctgagtat tttatctgcc tgactgtgtc cacacgtttt
     7261 gcaggtaggc gtaggtcagc ccggcggtgc cggagaccag cgcgatgtcg ctgcccaagt
     7321 cttcggcttt gcccacgccg acaggcaagg cgccggcggc tttgatggcg gctactcctg
     7381 ctgctgcgtc ttcgatgccg atacagcggc ggatgtccgc gcccacgccc tcggctgcgg
     7441 cgaggaagat gtcgggggcg ggtttggaat gcgcgacggc ggcagggtcg gcaatggcgt
     7501 cgaagaagtg ggtcagcccc atgcgttcga gcaggaacgg gccgttttta ctggcggacg
     7561 cgagggcgat ttttttgccg tttgccctca atgcttccag cagcggcaaa acgccggggt
     7621 acacgtcttc gggtttgacc gcctgaatca tctcgacgta gttgtcgttt ttgcggcggg
     7681 tcagttcggc aaactcggct tcgccgacgg ttttgccgcc gtgcgcgagg atgcgtttga
     7741 gcgaatcgtc gcgcgacacg cctttgagct gctcgttaaa cttgcggtcg atgccgatgc
     7801 ccagttcttc ggcgagcttt ttccatgcgc ggtagtggta ttcggcggtg tcggtgatga
     7861 cgccgtcgag atcgaagagc actgcggtaa acgtcatttt gcgccccctt atttttccaa
     7921 cgtgacggta tggctgccgt tgagcgtgat gtctttgccg tacacctgca attcgagcgg
     7981 ttcgcctttg agcagggtga agacgacgtt ttctttgccg acggcgactt taatcagacg
     8041 gcctcggtag ttgatgtgga aggcgtagcc tgtccacgca ctcggcagga acggtgcgaa
     8101 gctgagtgtg ccgccccaag ttttcatttg ggcgaaacct tggacgatgg cgagccacga
     8161 gccggtcatg gaggtaatgt gcaggccgtc ttcggtgtcg ttattgtagt tgtccaagtc
     8221 cagacgggcg gtgcgctggt acatttccac ggctttttct tctttgccca gttcggcggc
     8281 aaggatggca tgaatacacg gcgacaacga gctttcatgc acggtcatcg gttcgtagaa
     8341 gtcgaagttg cgacgttttt cgtccatatt gaaacggtcg ccgaagaagt agatgccttg
     8401 caagacgtcc gcctgtttga tgaagggcga acgcaggatt ttgtcccacg accatttctg
     8461 gttgagcggc aaatcgtcgg gcgaaagcgc ggacactggg cggatgtctt tgtcgaggaa
     8521 gccgtcgtgt tggacgaata cgccgagttc ttcgtcatga ggacggtaca tattcgcgct
     8581 gatgtccgcc catttttcca actcggcggc acgcacgttc aaatccggac gcgggtattt
     8641 cgccaaggct tcgcgggtgt agtccaatac ccaagcggca agggtgttgg tgtaccagtt
     8701 gttgttgatg ttgttttcgt attcgttcgg gccggttacg ccgtgaatca tgtatttacc
     8761 cctgcgtttg gagaagtgga cgcggtccgc ccagaagcgg gacacttcga ccaagacttc
     8821 caagccttct ttggcaagat agctttcgtc gccggtgtag ttggtgtagt tgtagatggc
     8881 gtaaggaatc gcgccgttgc ggtggatttc ctcgaaggtg atttcccatt cgttgtggca
     8941 ctcgatgccc gtaaacgtta ccatcggata gagtgcgccc gccaagccct gttcgcgcgc
     9001 gttgtgctgc gcctgcggca gttggttgcg gcggtattgc agcaggttgc gggtaacttc
     9061 gggttccgcc agtgcgaggt aaagcggcac ggcgtaggct tcggtgtccc aataggtcgc
     9121 gccgccgtat ttttcgccgg taaagccttt cgggccgatg ttcaggcgcg cgtcttcgcc
     9181 gtagtaggtg gagaacagtt ggaacaggtt gaagcggatg ccctgctgcg cttcgtcgct
     9241 gccttcgatg accacgtcgg cgatttccca acggtgcagc cagcctgctt tgtgcgcgtc
     9301 cagcaaggtt tcaaacgcaa cgcctgcgat tttttccgac aaggcgcggc ctgcggcttt
     9361 cactgcttcc aagccctgat aatcgcggct ggtggtaaca atcacgcgtt tttcaaaggt
     9421 ttcgggcgtg ccaccgactt cggcctcgaa agaattggag acctgccagt cggtttggct
     9481 gccgccgagg gctttgaagc tgccggcgaa agtttgctcg gcgttgacga taaattgttc
     9541 cacgccgaag ggattggtga cggtttgggt ggcaatgtag gagcggtcgt ctgaaacgcc
     9601 tttgtccaat acttgccaga atttttcttc gtagttggaa tcttcgtttt tcacgtcggc
     9661 atcgatgatg gaatcgatgc ggacttggtg ggttttgccg tcgatagata cggcttccca
     9721 gcggatgaga gccagctctt tttgtgcgac ggacaggaat ttgcacacat cgaaacgcac
     9781 gccgaatacg gtgaacgagc ggcgcaacac gccgtgctgc atatcgagtt cgacggagaa
     9841 gccggcaaca tcgtttttcg ccaagtccac ctcttgacca tcgacaaaga ttttgacttt
     9901 gctgaaattg agcgcgttga tggctttgcc gaaatatttg gggtagccgt ttttccacca
     9961 gccgacgcgg gttttgtcgg gaaaccacac accggcgatg taggtaccca agtggctgtc
    10021 ggcggaatag gtttcctcaa agctgccgcg catacccata tagccgttgc ccaaactggt
    10081 caggctctct tgcagccgtt tgtgttcttt ttccagcttt gccgaacgca gcgtccaagg
    10141 gctgatttcc ataattcttg tgtacattta tgaagctcct gtttggattg atttgaggga
    10201 atggtgaaat cttatagtgg attaacaaaa atcaggacaa ggcgacgaac cgcagacagt
    10261 acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
    10321 agttaaggcg aggcaacgcc gtactggttt ttgttaatcc actataaaaa ggccgtctgc
    10381 aaccggtgtt aggaagctcc taagaaaggg attcgatgcc gtaagcaatc gtcgcctccc
    10441 tagtatcacc cttgttcaga cgaatattgc cgaactcggg ccaattcagg ctgtcgggca
    10501 gcgtctgcgc ctcggtcgcc agcgcgtcgt aaacgcccgc atcgtgccgc gcgaaatcct
    10561 gcggggcggc ggtaaagatg accaagccgt tgcggtcgct gtatatgctg atacgacggc
    10621 ggcgtccggc ttgcaacaca gcggcgggac ggcctatatc ggacggcacg cggtaagcgt
    10681 cgtcaaaacc ggcccgaccc gtttcgcggc gcagggcggc aacggcggca tccagcggct
    10741 tgggccggct gaaatcaaat acttcgaggt cgtctgaaac cgttgagacg ggcagttttt
    10801 cggcatcggc cggaatatgt ccgccctgcg gaatatgcag aaccgcatcg tgcaggcccg
    10861 cgtccagccg ccagtaaatg tgcagcgtcg ggtcgaacac cgtgtcgccg agcgcggtgg
    10921 cgcgatagct aacggtaagc cggtcgtcct cgtccaagcg gtaggaaata tccaaatcca
    10981 aatcgttggg ataaccgtcg gccgactgtt gcaggcggct gcgcagcacc accgaacggc
    11041 cgtctgccgc caccgcgttg aaacgggtaa cggccagccc gtgcgaaccg ccgtgcagcg
    11101 cgttcctgcc ttcgttggcc tccacgcggt aagtcctgcc gttgatgtcg aacgccgcac
    11161 cgcggatgcg tccggccacg cgccctatct gcttgttaat ctgaaacgga ttgtccgcat
    11221 aggaagccgc atcgtcgaac gacaccacga ggttttcgcg cacgccgtct gccaaaacgg
    11281 aaaattcctg cacaatcccg cccaagtcca gcacgcagac acgcgtacca cgccgattgg
    11341 acagcacata gctggttacg gcacgcccgt cgatcaggcc gaaatcgcgg gtagcggggg
    11401 tagcgggggt atcgctcatc gctcaaaccc cgccgtgtgt ttctttaatc aggaacacgg
    11461 aaaacgcgcc cagcagcagg acgacgcccc ctaccaagaa catagtggcc tgcaagccgc
    11521 ccagcatagg gaaaagcacg aaactcaaca gcgaagcgac gatttgaggc atacagatag
    11581 agccgttaaa caggcccaag taagtgccca tatgcttgcc cgacaaggcg ttggtcacaa
    11641 tcgtcagcgg ataagtgata atgcccgccc aagcgatgcc gattaaggta taagacaaca
    11701 ccagcgcgta ttggttgccg atgaagaaaa cggagaaaaa gccgagcgcg cccaaagcca
    11761 aacagccgaa ataacccgcc ttatggtatt tattcggcac tttcgccaat acaaacgaac
    11821 aaatcaccgc cgcaaccgac tgcaccgccg ccaaaacgcc gtaccagttg cccgcttctt
    11881 ggtaacctac ggaagacgca tcggtggtgt gccagacgtt ttccgcaatc gcgcctgccg
    11941 agtaagtcca catatattgg aaggcgaacc agcagaagaa ttgcaccaaa gtaaccgtcc
    12001 aaaacgcctt aggcgcggtt ttcaagagtt cgatccagtt ggctttttcc tgattcgcgg
    12061 cgacatcgat gccgtggtaa cgggcgtagg tttccggatc gtattccttc actttgaaaa
    12121 tcgtgaacgc gctggtaatc accagcaacg ccgcacccac ataaaacgcc acgaccacgg
    12181 tctgcggcac aacgcctttc tcggcggtgt tcgccaaacc gatatacgca aacacaaacg
    12241 gcagaatcgc cgccacgacc gcgcccgtat ttgctaagaa actttgaatc ccgtaggcgt
    12301 agcctttctg ctcctcgttg accatgtcgc cgaccatcat cttaaacggc tgcatcgcca
    12361 tatttgacga cacgtccaac agcgcaatca tcagcgcgcc gaacgacaaa gccgccagcg
    12421 acgcatagcc gaaaccgaag ctgcccgagt tcggcatcaa aatcatcaca ataaccgcaa
    12481 tcagcgtgcc ataaagcaga tacggcagac ggcggccgcc caaacgcggc ttccaagtgc
    12541 ggtcggagta atggccgaca atcggctgca ccagcatccc cgccagcggc ggcaggatga
    12601 aaaaccagcc caaattgtgc gggtctgcgc ctagcgtttg aaaaatgcgg ctcatttgcg
    12661 agctttgcag ggtaaaggcc gtctgaacgc cgagaaagcc gaaactgagc atccaaatcg
    12721 tgctttttgc cagcgcgggc aaaccttgtt ttgctgtttg aggcgtatat tccgacataa
    12781 ggtaaatcct tttttgattt gaaaagtata gt
//
LOCUS       DAWFJW010000061        12419 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFJW010000061 DAWFJW010000000
VERSION     DAWFJW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.061"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..1160
                     /locus_tag="WGR19_001310"
     CDS             <1..1160
                     /locus_tag="WGR19_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0798838.1"
                     /translation="AKNKDKSTFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTED
                     KREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFI
                     LPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIF
                     YKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESDIVLDYHLGSGTTAA
                     VAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFN
                     ETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQEPEFQSLALDEQKQM
                     VLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQAEKKDGE"
     gene            1150..3930
                     /locus_tag="WGR19_001311"
     CDS             1150..3930
                     /locus_tag="WGR19_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0798839.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHSKFLFTEKILQGDQVIPIHKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATPPEKADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     VDESKADYLAFLNWVENVQADDFSFLKTFSMSLNDSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWIKQNYQKHNIIITNSETNKSKTEKTDSETEKLLNNLEAVDNPIRAIFTV
                     GRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFSFED
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNENFKNLLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(4074..5246)
                     /locus_tag="WGR19_001312"
     CDS             complement(4074..5246)
                     /locus_tag="WGR19_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0798840.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            5537..5983
                     /gene="iscR"
                     /locus_tag="WGR19_001313"
     CDS             5537..5983
                     /gene="iscR"
                     /locus_tag="WGR19_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0798841.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNSDGSRVVQFTHIH"
     gene            6012..7226
                     /locus_tag="WGR19_001314"
     CDS             6012..7226
                     /locus_tag="WGR19_001314"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0798842.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7487..7873
                     /gene="iscU"
                     /locus_tag="WGR19_001315"
     CDS             7487..7873
                     /gene="iscU"
                     /locus_tag="WGR19_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0798843.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            7960..8280
                     /gene="iscA"
                     /locus_tag="WGR19_001316"
     CDS             7960..8280
                     /gene="iscA"
                     /locus_tag="WGR19_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0798844.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(8240..8461)
                     /locus_tag="WGR19_001317"
     CDS             complement(8240..8461)
                     /locus_tag="WGR19_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0798845.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            8543..9043
                     /gene="hscB"
                     /locus_tag="WGR19_001318"
     CDS             8543..9043
                     /gene="hscB"
                     /locus_tag="WGR19_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0798846.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            9141..11891
                     /gene="gyrA"
                     /locus_tag="WGR19_001319"
     CDS             9141..11891
                     /gene="gyrA"
                     /locus_tag="WGR19_001319"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0798847.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            12048..12419
                     /locus_tag="WGR19_001320"
                     /pseudo
     CDS             12048..12419
                     /locus_tag="WGR19_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3776 a         3066 c         2748 g         2829 t
ORIGIN      
        1 ttgctaaaaa taaagataaa tctacttttt atgaatttcc aatagatgaa gataattttt
       61 tagagaaatg ggaagaagat gaaattgggt tttataaaaa aggagctcca atgcgagcaa
      121 caggaaccga agataaaaga gaagatagac ctgaaatgtt ctatcctttt ttggtaaaaa
      181 ataacacagt tagtacaatt acagatgaag aattttctca aatctataat aaagatttag
      241 aagtattcaa tgatgatttt attcaaaaac taaaagagaa atacgaaaat cttggataca
      301 attttatttt acctattgct gataaacaat gggggcgttg gcgttggggg tattcaataa
      361 agaataaggc aaggctgcag acggatatta ttgttagcca atctaaaaat ggaatttcct
      421 tgtataaaaa acaaagacct gaattagatg atttacctac aaaaaaacct aaaactattt
      481 tttataaacc tgaatacagt agtggtaatg gaactgaaca gatgaaaaat ctttttggtg
      541 aaaaggcatt taaaaatcct aaaccagaag aacttatcca agattttatt accatcacaa
      601 ccaacgaatc cgacatcgtc ttagactacc atcttggtag tggcacaacc gccgccgttg
      661 cccacaaaat gaaccgacaa tatatcggta ttgaacaaat ggattatatt gaaacgcttg
      721 ccgttgaacg cttgaaaaaa gtgattgatg gcgagcaagg cggtatttcc aaagccgtga
      781 attggcaagg tggcggcgag tttgtctatg ccgaacttgc tccatttaac gaaaccgcaa
      841 aacaacaaat tttggcttgc gaagattcag acggcatcaa aacgctgttt gaaggtttat
      901 gcgaacgcta tttcttgaaa tacaacgtca gcgtaaatga atttagtcaa atcattcaag
      961 agcctgaatt tcaatccttg gcattagacg aacaaaaaca aatggtgctt gaaatgttgg
     1021 atttaaatca aatgtatgtt tcattatccg aaatggatga tgagcaattt gcaggttgct
     1081 taaatgatga cgataaagcg ttaagccgtg aattctatca atcagcaaaa aatcaagcgg
     1141 agaaaaaaga tggcgaataa taaaacgtta tttgattggg tggaagaccg caaatcaacg
     1201 ctggaagaaa tagaacagac agattttttc gcgctgcctg aatttgtaga acgcaattta
     1261 aaatatccgt tttttgaatg gcaaaaatcg gctttagaaa actttgtgat ttttgaccgc
     1321 gcttcaaaat taaaggattt ccctgacatc aaaaaccgcc ctacccattt gctgttcaat
     1381 atggcaacag gtgctggcaa aacgatgatg atggcggcgt tgattttgta ttattttgaa
     1441 aaaggttatc ggcatttttt gtttttcgtc aatcaaaata atatcgtgga taaaacggaa
     1501 aataatttta ttgacccggc gcactcaaaa tttttattta ccgagaagat tttgcaaggc
     1561 gaccaagtta ttcccattca caaagtagaa acgtttagcc cacattcaga cggcattgaa
     1621 attaaattta ccagtattca aaaactgtat aacgatatcc acaccgaacg ggaaaaccaa
     1681 accacattgg cagatttgca cgaattgaac cttgtgatgc tgggcgatga agcgcaccat
     1741 ttaaacgcac aaaccaaagg caaaaaacaa ggcgagttgg atttggaagt ggaattaaaa
     1801 gccaatgcag gcgatgccga atttgaacgc aaaggctggg agcatatggt tttggaattg
     1861 ttgctcaata aaaatggcaa tcccggccaa aatgtgctgt tggaatttac cgccacgcca
     1921 cctgaaaaag ctgacgtgca acaaaaatac gctgataaaa tcatcacaaa atttggttta
     1981 aaagaatttt tgcaaaaagg ttataccaaa gaaatcaatt tggtatccag tacactgagc
     2041 aagaaagagc gggtgttgca cgctttattg tttgcttggt atcgccatca aatcgctttg
     2101 aaatatggca ttgccaattt caagcctgtg atgttgttta gaagtaagac ggttgatgaa
     2161 tcaaaagcgg attatttggc atttttaaat tgggtagaaa atgtgcaagc agatgatttt
     2221 tcgtttttaa agacattttc aatgagctta aacgatagcg ataacgccaa cgaacaaggc
     2281 aaaacccgca ccgaacaagc cctaaaattt atgcaggaaa atggctttga gtttgcacat
     2341 ttagcaaatt ggataaaaca aaattatcaa aaacacaata tcattattac caactccgaa
     2401 accaacaaaa gcaaaaccga aaaaaccgac agcgaaacgg aaaaattgct aaacaatctg
     2461 gaagcggttg ataatccgat tcgtgctatt tttacggtgg gtagattaac cgaaggttgg
     2521 gacgttttaa atttgtttga tattgtgcgt ttgtatgaag ggcaaaacgg cggcggttca
     2581 aataaaaaat catgcaaaac ggcaaataca acggtatcag aaaaacagtt gattggtcga
     2641 ggcgtgcgtt attttccatt ttcgtttgaa gataaacagc cgaataagcg taaatttgac
     2701 aacgacatgc aacacgaatt gcgtattttg gaagaattgt tttattacac gcacgatgag
     2761 caatcccgct atattacaga actgaaaaac gagttacgaa aagacggtta tttgcctgaa
     2821 aaagacgatg ataaggtatt ggcaacattt aaactcaaat ctgaatttgc agataatgaa
     2881 aattttaaaa acttattgat ttgggcgaat aaaaaaatcc ccaacccaaa cgccaaaacc
     2941 aataatgcaa acagcctgca agccaatccg caaacgcttt cattccaagt tcacggcaat
     3001 caactgttgc aggaaacgca atttacagcc gatgaaaatg atgaaaccgc ccgacaaatc
     3061 ggcacacaaa ataattttac tcaaaccata aaaatgagtg aaatggagcg gcatattttc
     3121 aataaagcat tacatatcaa agggaaaaat agtcaatctt tattccattt caaccgtttg
     3181 caaagcaagc tcaacattca aaatcgcaat gaattgcaaa ataacttgtt gaaagactgg
     3241 caaattgaat ttttaggatt agggcaagac aaacaggttc gcccggacga taaacttgca
     3301 ggctgcctga aaatcttgga aatggttgaa aaacatctga atgaaagtga tatgccgttt
     3361 atcggtacaa aagaatttac gcctaaaaca ttatgggaaa ttttcggcac accaaaacaa
     3421 aaatgggtca aaaaagatga tgtaaaaacg actattgcca cgcaaaatga ttggtatgtg
     3481 atgaataatt ttgccggaac gggtttggaa gaagcgttaa ttcaatttat ttcagcgcgt
     3541 ttgggcgatt tgaaatctca atatgacatt catttaatcc gtaatgaaga agtgtttaaa
     3601 ttgaataact ttgccgatgg tgaaggattt atgccggact ttattttatt gctgaaagat
     3661 aaacaaaaat cttcttccaa tggcgtgaat gactttttgc attaccaaat tttcattgag
     3721 cccaaaggtg agcatttagt ggaaactgat ggatggaaag aagaattttt agaagccatc
     3781 actgtggaat atgggatgga taagatcctg caaaaagata caccgcatta tcgtttgatc
     3841 ggtttaccgt tttttactga tcatcagaaa aatggacaat ttacggagtc attcccttta
     3901 ggtgcggcat cgcttgaaaa aatgaagtag tgcattgcag gcagccccat ttgacaaaac
     3961 ttcctttaca aaagggcgtt ttgtcagata tttaatcaac acattattaa aatacagcca
     4021 aattttaatg ccgtctgaac cctgtgttca gacggcatcg tatttttcag tatctaaacc
     4081 gtttccctac cccaatcctt acccctcaaa atcgaggcat cgacatcttg aatatcgcgg
     4141 tgtcccgtaa acgccataga tatatccatt tctttatata ggatttccag cgcacgggtt
     4201 acgccttctt cgccatacgc gcccaagcca tacaggaacg cccgacctat cattgtacct
     4261 ttcgcgccca aagcccaagc cttcaaaata tcctgaccgc tgcggatgcc gctgtccatc
     4321 caaacttcga tgtcgctgcc cactgcgctg acgatgtcgg gcaaggcttt gatggcagac
     4381 acggtatcgt cgagctgtcg gccgccgtgg ttggaaacaa tcaatgcgtc cgcgccgctt
     4441 ttcgctgctt tttccgcgtc ttcgggttcc ataatgcctt tgataatcag cttgccgccc
     4501 cacaaatctt taatgcgcgc cacatcgtcc cagctcaggc gcgggtcgaa ttgttcggaa
     4561 gtccatgaag acagcgaaga caaatcgccg acgttcttcg cgtgtccgac gatattgcgg
     4621 aacgtgcggc gttccgtgtt cagcattttc atacaccatt cgggcttggt cgccagattg
     4681 attaaattgg cgatggtcgg tttcggcggc gcggacaggc cgtttttgat gtctttgtgg
     4741 cgttgcccca aaacctgcaa atcagcggtc aataccaatg ccgaacattt ggcatccttc
     4801 gcgcgcttaa tcaggttttc cataaactcg cggtcgcgca tcacataaag ctgaaaccaa
     4861 aatggtgcgg aagtgttctc ggcaacgtct tcaatcgagc agatagacat cgtggacagc
     4921 gtaaacggaa tgccgaactt ctccgccgcc cgcgccgcca aaatttcacc gtcggcgtgt
     4981 gccatacccg tgaaacccgt cggcgcaatc gccaccggca ttttcacatc ctgcccgatc
     5041 attttggttt ccaggcttcg gccttccata ttgaccaata ccttttgtcg gaagcggatg
     5101 tctttgaaat ccgaagtgtt ttcacggtag gtagtttctg tccacgaacc cgaatcgatg
     5161 taatcgtaaa acatacgcgg cattttgcgc ttggcaacgc ggcgcaagtc ttcgatgcag
     5221 gtcattttgc tcaaatcacg tttcatttgt cgccccctga atacctgaat aactttatat
     5281 gaaatcgata atgtatatca atattgatta taaggcaaat catttcaaca tttgccgcat
     5341 ccgccgcagc tccctacttt aagcaacata aggtttaaaa ttcaaaaata acaaattaaa
     5401 atcaaaatat taaaaatcaa tcaatctgcc gatttaaaca gccaatcgcg caatccgccc
     5461 ccatacttga ctgaaacact cagatattgg acaattccgc ccactataaa aaaagccgac
     5521 acgggcaacc accaccatga gactgaccac caaagggcgt ttcgccgtta ccgctatgct
     5581 ggatttggcg atgaacgcgc aaaccggcgc cgtcaaactc agtgccatca gcgaacgcca
     5641 aaacatatcc ctctcctatc tcgagcaatt gttcggcaaa ctccgccgcg ccggactggt
     5701 tgaaagcctg cgcgggcccg gcggcggcta catcctcgcc gcaccggcgg cacgcatcaa
     5761 catcgcccaa atcatcgccg ccgccgaaga ccggctggac gcaacccaat gcggcagcaa
     5821 agccaactgc caccacggcg cgccctgcct gacgcacgat ctttgggaga atttaaacaa
     5881 aaccatcaac gactacctcg gcagcgttac cctgcaaagc atcatcgaac agaaaaacaa
     5941 cagcgacggc agccgcgtcg tccaatttac acacatccat taaataacac ccgaaaaaga
     6001 aagagcaaac catgaccgtc aaaacccccg tttacctcga ctacgccgcc accacccccg
     6061 ttgacaaacg cgttgccgaa aaaatgattc cctatctgac cgaaaccttc ggcaacccag
     6121 cctccaacag ccacagcttc ggctgggaag cagaagaagc tgtagaaaaa gcacgtgcag
     6181 acattgccgc cctgattaac gccgactcta aagaaatcgt tttcaccagc ggcgcaaccg
     6241 agtccaacaa cctcgctatc aaaggcgcgg cgcacttcta caaatctaaa ggtaatcacc
     6301 tcatcactgt aaaaaccgaa cacaaagccg tactcgacac catgcgcgaa ctcgaacgcc
     6361 aaggttacga agtaacttat ctggacgtac aagaaaacgg tttggttgat ttagacgtac
     6421 tgaaagccgc catccgcgaa gacaccatcc tcgtttccgt aatgtgggta aacaacgaaa
     6481 tcggcgtggt gcaagacatt cctgccatcg gcgaaatctg ccgcgaacgc aaaatcattt
     6541 tccacgttga cgcagcacaa gcatgcggca aagtgcctgt tgatgttgaa gccgcaaaag
     6601 ttgatttgct gtctatgtcc ggccacaaag tatacggccc taaaggcatc ggcgccctgt
     6661 atgtacgccg taaaccacgc gtccgcctcg aagcccaaat gcacggcggc ggtcacgaac
     6721 gcggtttccg ttccggcaca ttgccgaccc accaaatcgt cggcatgggt gaagccttcc
     6781 gcattgccaa agaagaattg gcacaagaca ctgcacacta cctgaaactg cgcgatattt
     6841 tcctcaaagg tatcgaaggc atcgaagaag tctatatcaa cggcgacctc gaacatcgcg
     6901 tcccgaacaa cctaaacgtc agcttcaact tcgtcgaagg cgaaagcctg attatggcag
     6961 tgaaagaact cgccgtatcc agcggctccg cctgcacctc cgcctcgctc gaacccagct
     7021 acgtcctgcg cgcattgggc agaaatgacg aattggcgca ctcgtccctg cgcatcacct
     7081 tcggtcgcat gaccactgaa gaagaagtgc aatttgccgc cgaactgatt aaatccaaaa
     7141 tcggcaaact gcgcgaactg tcgccgttgt gggaaatgtt caaagacggg attgatttga
     7201 actcgattga atgggcggcg cattaaagcg taccaacatg ccatccgaac cttcagacgg
     7261 cattccaaaa acaaagcaat caagagaaaa atatgaacga acaagattta gatttggaca
     7321 acctcgacaa cctgcttgac gatttcgacg gcgttaccgt ggaaggcggc gtcgattcgg
     7381 aaaacgacga cggctgtgaa ggcggagcgt gcaaaatcta actcaggccg tctgaaaacc
     7441 ggcaaacaaa ccacttaaat catcaaaaca ttaaggaaac cacatcatgg catacagcga
     7501 taaagtaatc gaccactacg aaaatccccg caacgtcggt actttcgaca aaaacgacga
     7561 atctgtcggc accggcatgg tcggcgcacc ggcctgcggc gacgtgatgc gcctgcaaat
     7621 caaagtgaac gatgaaggca tcatcgaaga tgcgaaattc aaaacttacg gctgcggttc
     7681 ggccatcgct tcgtccagcc tgattaccga gtgggtcaaa ggcaaaagtc tggatgacgc
     7741 gctggcaatc aaaaacagcg aaatcgccga agagttggaa ttgccgccgg taaaaatcca
     7801 ctgctccatc ttggctgaag atgcggtaaa agcggccgtt gccgactacc gcaaacgtca
     7861 ggaaaacaga taaagccctt cagacggcat cgtcccgcaa tgccgtccga accacccgcc
     7921 gcttcgggtc tgtccggggc ggtacaacaa ggaagaaata tgattaccct taccgagaat
     7981 gccgcaaaac acatcaatga ctatctcgcc aaacgcggca aaggcttggg cgtacgcttg
     8041 ggtgtgaaaa ccagcggctg ctcggggatg gcgtacaacc ttgaatttgt cgatgaagcc
     8101 gatggcgacg acctgatttt cgaaggacac ggcgcgcgca tttatatcga tccgaaaagc
     8161 ctggtttatc tggacggcac gcaagtcgat tacaccaaag aaggtttgca ggaaggtttc
     8221 aaatttgaaa accccaatgt caaagactcc tgcggctgcg gcgaaagctt ccacgtttaa
     8281 ggcataaaaa cggcgggaca gtatcaaaac cgtcccgcca ttttttcgct tcctgcctgt
     8341 tgtagctgcc tttgcctttc cttttccgtt ccaccttgtg ccggaacaaa tcggatttca
     8401 ctaaggcttt taaagcattg tcgcgtattt tgcctttatt gtgctgcact ttgccgccca
     8461 tattcagtcc tttcgtttaa gaagctgcag attataaggc aaaaacagtt ttctgccaaa
     8521 atcttacatt tatcatccta ctatgtccca atatttcacc ctcttccgga ttgaacccgc
     8581 tttcgatatc gataccgaaa acttggaaca aacctaccgc gccttggccg cccgtttcca
     8641 tcccgataaa ttcgcttcag cttccgcctt tgagcaaaag caggcagtga tgatgtcttc
     8701 caccatcaac gatgcctacc gcaccttgaa aaaccccatc gaccgcgccg cctacctgct
     8761 gaaaaaatcg ggcatcgatg ccgacgcgcc ggagcatacc gctttcgccc ccgaattcct
     8821 tatgcagcaa atggaatggc gcgaaacgct gatggaggca cgggcaggca acgaccttga
     8881 atccttgaaa aatctcgaca acgaaatccg cgacgagcaa gaaaaactgt tctgcggtct
     8941 gaaacagtcg tttgcccgac aagattacga cacagccgca caacaagtcc gccaaggcag
     9001 gtttctcgac aaactccgca acgaaatttc ctcggcatta taatccgcac cgtgtttcag
     9061 acggcgtaac cgccgcaccg ttccgcgtca aaatatgcta aaataagcaa caattttttg
     9121 ccatacgaaa cattgaaacc atgaccgacg caaccatccg ccacgaccac aaattcgccc
     9181 tcgaaaccct gccggtaagc cttgaagacg aaatgcgcaa aagctatctc gactacgcca
     9241 tgagcgtcat tgtcgggcgc gcgctgccgg acgttcgcga cggtctcaag ccggtacacc
     9301 gccgcgtact gtacgcgatg cacgaattga aaaacaactg gaatgccgcc tacaaaaaat
     9361 cggcgcgcat tgtcggcgac gtcatcggta aataccaccc ccacggcgat accgccgtat
     9421 acgacaccat cgtccgtatg gcgcaaaatt tcgctatgcg ttatgtgctg atagacggac
     9481 agggcaactt cggatcggtg gacgggcttg ccgccgcagc catgcgctac accgaaatcc
     9541 gcatggcgaa aatttcccac gaaatgctgg cagacattga ggaagaaacc gtcaatttcg
     9601 gcccgaacta cgacggtagc gaacacgagc cgcttgtact gccgacccgt ttccccacac
     9661 tgctcgtcaa cggctcgtcc ggcatcgccg tcggcatggc gaccaatatc ccgccgcaca
     9721 acctttccga taccgtcaat gcctgcctgc gcctgctcga tgcacccgac accgaaatcg
     9781 acgaactgat cgacattatc caagcccccg acttcccgac cggggcaacc atctacggct
     9841 tgagcggcgt gcgcgaaggc tataaaacag gccgcggccg cgtcgttatg cgcggtaaga
     9901 cccatatcga acccataggc agaaacggcg aacgcgaagc catcgttatc gacgaaatcc
     9961 cctatcaggt caacaaagcc aagctggtcg agaaaatcgg cgatttggtt cgggaaaaaa
    10021 cactggaagg catttccgag ctccgcgacg aatccgacaa atccggtatg cgcgtcgtta
    10081 tcgagctgaa acgcaacgaa aatgccgaag tcgtcttaaa ccaactctac aaactgactc
    10141 cgctgcaaga cagtttcggc atcaatatgg tggttttggt cgacggacaa ccgcgcctgt
    10201 tgaacctgaa acagattctc tccgaattcc tgcgccaccg ccgcgaagtc gttacccgac
    10261 gtacgctttt ccggctgaag aaggcacgcc atgaagggca tatcgccgaa ggcaaagccg
    10321 tcgcactgtc caatatcgat gaaatcatca agctcatcaa agaatcgccc aacgcagccg
    10381 aggccaaaga caaactgctt gcacgtcctt ggcgcagcag cctcgttgaa gaaatgctga
    10441 cgcgttccgg tctggatttg gaaatgatgc gtccggaagg attggctgca aacatcggct
    10501 tgaaagagca aggttattac ctgagcgaga ttcaggcaga tgctatttta cgcatgagcc
    10561 tgcgaaacct gaccggcctc gatcaagaag aaattgtcga aagctacaaa aacctgatgg
    10621 gtaaaatcat cgactttgtg gatatcctct ccaaacccga acgcattacc caaatcatcc
    10681 gcgacgaact ggaagaaatc aaaaccaact atggcgacga acgccgcagc gaaatcaacc
    10741 cgttcggtgg cgacattgcc gatgaagacc tgattccgca acgcgaaatg gtcgttaccc
    10801 tgacacatgg cggctatatc aaaacccagc cgaccaccga ctatcaggcg cagcgtcgcg
    10861 gcgggcgcgg caaacaggcg gctgccacca aagacgaaga ctttatcgaa accctgtttg
    10921 ttgccaacac gcatgattat ttgatgtgct ttaccaattt gggcaagtgt cattggatta
    10981 aggtttacaa actgcccgaa ggcggacgca acagccgcgg ccgtccgatt aacaacgtca
    11041 tccagttgga agaaggcgaa aaagtcagcg cgattttggc agtacgcgag ttccccgaag
    11101 accaatacgt cttcttcgcc accgcgcagg gaatggtgaa aaaagtccaa ctttccgcct
    11161 ttaaaaacgt ccgcgcccaa ggcattaaag ccatcgcgct caaagaaggc gactacctcg
    11221 tcggcgctgc gcaaacaggc ggtgcggacg acatcatgct gttctccaac ttaggtaaag
    11281 ccatccgctt caacgaatac tgggaaaaat ccggcaacga cgaagcggaa gatgccgaca
    11341 tcgaaaccga aatttcagac ggcatcgaag atgaaaccgc cgacagcgaa aacgcactgc
    11401 cgagcggcaa acacggtgtt cgcccgtccg gtcgcggcag cggcggtttg cgcggtatgc
    11461 gcctgcctgc cgacggcaaa atcgtcagcc tgattacctt cgcccctgaa accgaagaaa
    11521 gcggtttgca agttttaacc gccaccgcca acggatacgg aaaacgcacc ccgattgccg
    11581 attacagccg caaaaacaaa ggcgggcaag gcaatattgc cattaacacc ggcgagcgaa
    11641 acggcgattt ggtcgccgca accttggtcg gcgaaaccga cgatttgatg ctgattacca
    11701 gcggcggcgt acttatccgc accaaagtcg aacaaatccg cgaaaccggc cgcgccgcag
    11761 caggcgtgaa actgattaac ttggacgaag gcgaaacctt ggtatcgctg gaacgtgttg
    11821 ccgaagacga atccgaactc tccgacgctt ctgtaatttc caatgtaacc gaaccggaag
    11881 tcgagaactg aaaatcatct cccgatgccg tctgaagatt cagacggcat ttattttatc
    11941 cctcatccgt catccagctt ctcacaatat agcggattat agtcaattaa aaacaagggg
    12001 ctgtcctaga taactaggga aattcaaatt aagttagagt tgcccctatg agaaaaagtc
    12061 gtctaagccg gtataaacaa ataaactcat tgaactgttt gtcgcaggtg taactgcaag
    12121 aacgacagca gagttagtag gcgttaataa aaataccgca gcctattatt ttcatcgttt
    12181 acgattactt atttatcaaa acagtccgca tttggaaatg tttgacggca aagtagaagc
    12241 agatgaaagt tattttgctg aacgacaaaa ccatatcaat ggaattggga acttttggaa
    12301 ccgggcaaaa cgtcatttac gcaagtttga cggcattccc aaagcgcatt ttgagctgta
    12361 tttaaagggg tacgaacggc gttttaacaa cagtgagata aaagttcaaa tttccattt
//
LOCUS       DAWFJW010000062        11775 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFJW010000062 DAWFJW010000000
VERSION     DAWFJW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.062"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            38..461
                     /locus_tag="WGR19_001321"
                     /pseudo
     CDS             38..461
                     /locus_tag="WGR19_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WGR19_001322"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WGR19_001322"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0798848.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTVAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WGR19_001323"
     tRNA            1210..1285
                     /locus_tag="WGR19_001323"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="WGR19_001324"
     tRNA            1311..1386
                     /locus_tag="WGR19_001324"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="WGR19_001325"
     tRNA            1410..1485
                     /locus_tag="WGR19_001325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="WGR19_001326"
     tRNA            1507..1582
                     /locus_tag="WGR19_001326"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="WGR19_001327"
     tRNA            1608..1681
                     /locus_tag="WGR19_001327"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1784..1873
                     /locus_tag="WGR19_001328"
     tRNA            1784..1873
                     /locus_tag="WGR19_001328"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Leu,seq:taa)
     gene            1977..3029
                     /gene="sohB"
                     /locus_tag="WGR19_001329"
     CDS             1977..3029
                     /gene="sohB"
                     /locus_tag="WGR19_001329"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0798849.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3080..3946)
                     /locus_tag="WGR19_001330"
     CDS             complement(3080..3946)
                     /locus_tag="WGR19_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0798850.1"
                     /translation="MWKMLKHIAQTHRKRLLGTFSLVGLENLWMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSKRNIKAGT"
     gene            complement(3956..4387)
                     /locus_tag="WGR19_001331"
     CDS             complement(3956..4387)
                     /locus_tag="WGR19_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0798851.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(4665..5534)
                     /locus_tag="WGR19_001332"
     CDS             complement(4665..5534)
                     /locus_tag="WGR19_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0798852.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(5629..6546)
                     /gene="hisB"
                     /locus_tag="WGR19_001333"
     CDS             complement(5629..6546)
                     /gene="hisB"
                     /locus_tag="WGR19_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0798853.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(6543..7640)
                     /locus_tag="WGR19_001334"
     CDS             complement(6543..7640)
                     /locus_tag="WGR19_001334"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0798854.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(7686..8975)
                     /gene="hisD"
                     /locus_tag="WGR19_001335"
     CDS             complement(7686..8975)
                     /gene="hisD"
                     /locus_tag="WGR19_001335"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0798855.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(8972..9871)
                     /locus_tag="WGR19_001336"
     CDS             complement(8972..9871)
                     /locus_tag="WGR19_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0798856.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(9975..10628)
                     /locus_tag="WGR19_001337"
     CDS             complement(9975..10628)
                     /locus_tag="WGR19_001337"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0798857.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(10707..11360)
                     /locus_tag="WGR19_001338"
     CDS             complement(10707..11360)
                     /locus_tag="WGR19_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0798858.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            11636..11720
                     /locus_tag="WGR19_001339"
     tRNA            11636..11720
                     /locus_tag="WGR19_001339"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11670..11672,aa:Leu,seq:caa)
BASE COUNT         2597 a         2807 c         3318 g         3053 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgccctcaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccccatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgtcgccg tcatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatta tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaaca tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa catatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 attatcgtac tacggcgggg tggcagagtg tttatgctat gaggagtgca atcttcatat
     1741 aggccggtta aaatccgcgc ccccgcctcc acctttcaca aatgcccggg tggtgaaata
     1801 ggtagacaca acggacttaa aatccgtcgg gactaaacat cccgtgccgg ttcgattccg
     1861 gctccgggca ccaagctgaa aatgacaatg ccgctccaag cggttatttt tttatctgtc
     1921 ggacggtgat ggacaatatt gacatgttca tgcctgaaca agaggaaatc caatcaatgt
     1981 ggaaagaaat tttactgaat tacggtattt tcctgctcga actgcttacc gtgttcggcg
     2041 caattgcgct gattgtgttg gctatcgtac agagtaagaa acagtcggaa agcggcagtg
     2101 tcgtactgac ggatttttcg gaaaattata aaaaacagcg gcaatcgttt gaagcattct
     2161 ttttaagcgg ggaagaggca aaacatcagg aaaaagagga aaagaaaaag gaaaaggcgg
     2221 aagccaaagc agagaaaaag cgtttgaagg agggtgggga gaaatcttcc gaaacgcaaa
     2281 aatcccgcct ttttgtgttg gattttgacg gcgatttgta tgcacacgcc gtagaatcct
     2341 tgcgtcatga gattacggcg gtgcttttga ttgccaagcc tgaagatgag gttctgctta
     2401 gattggaaag tccgggcggc gtggttcacg gttacggttt ggcggcttcg cagcttaggc
     2461 gtttgcgcga acgcaatatt ccgctgaccg tcgccgtcga taaggtggcg gcgagcggtg
     2521 gttatatgat ggcgtgtgtg gcggataaaa ttgtttccgc tccgtttgcg attgtcggtt
     2581 cggtgggtgt tgtagcggaa gtaccgaata tccaccgcct gttgaaaaaa catgatattg
     2641 atgtggatgt gatgacggcg ggcgaattta agcgcacggt tacttttatg ggtgaaaata
     2701 cggaaaaggg caaacagaaa ttccgacagg aactggagga aacgcatcag ttgttcaagc
     2761 agtttgtcag cgagaaccgc cctcaattgg atattgagga agtggcaacg ggcgagcatt
     2821 ggttcggtcg gcaggcgttg gcgttgaact tgattgacga gatttcgacc agtgatgatt
     2881 tgttgttgaa agcgtttgaa aacaaacagg ttatcgaagt gaaatatcag gagaagcaaa
     2941 gcctgatcca gcgcattggt ttgcaggcgg aagcttctgt tgaaaagttg tttgccaaac
     3001 ttgtcaaccg gcgggcggat gtgatgtagt ttgccgaacg gatggaaatg ccgtctgaag
     3061 cgtgttcaga cggcattttt caagttccgg ctttgatgtt ccgtttcgac cactctatcc
     3121 gttgtccgat gtctttcaaa ttggaatatt gttcgaccaa tcgcggcacg tcgtccaaac
     3181 tcatggcaaa catccacaga taagtgccga ccgaatagac atgccccgcg ctgctgtagc
     3241 ctttgagcgt catcatcaca aaagcaaagc cgaacaaaat acccatcgcc gtgccgacgc
     3301 agagatagcc gaaggcttcg cggttggaaa tcagcacacg caggcgcgca agcagtccgt
     3361 aatggcggtc cagctgccgc tcgtcgcctt ttcggataaa gtggttgtcg cgttccaagc
     3421 tgttcttcag gcggaaatac aggttttcgc tgatggcggc aaaacgtggc aaaagccata
     3481 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
     3541 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
     3601 aaaagctgac aaactcacgc gacagggcaa cccgcgcagt taccgccgaa tgcgggactt
     3661 gtcgctgccg ctgttccaac acgaccggca cggcgatttc ggtataaatc cgcgtaaacg
     3721 tgcgcgtatc ggcaatccgc cgcgccgcac cgaccagcca catcaaaagc acaaccaaag
     3781 cgtacagcaa cgcctgccac acctcccccg caatcacggc attgatcgcc cggccgccaa
     3841 acactggata taccagcatc caaaggtttt ccagtccgac cagggaaaac gtgccgagca
     3901 gacgcttgcg gtgggtttgg gctatgtgtt tcaacatttt ccacatattt gctccctatt
     3961 ttttattttc cgagattgtt ttttccatca cttcagccag tgcatccaaa tcggcaaaca
     4021 gccgggttgt gcgcttgtcg ccgaatgtgg caaatacttt gtcgctgaat tcctgcgcgc
     4081 tttctgttaa aggtgcggca tgggctttgc ctttttcggt cagcgacagc aaccgttcgc
     4141 gccggtcctg ttcgctttcc tgccattcaa tcaacccttg tccggcaagg gttttgcata
     4201 cgcctgaaac ggtctgtttg ggcaaactcc actcttcgcc gatatgcttt tgcgtgcggc
     4261 tgccttcggt tgccagggta taaagtacgg caaagaggtt gtaattcaga tcctgctgcc
     4321 cgatccattt gtcgaagaca ttgcaaatca ggttgatacg ggtgccaagt tggtcgagtt
     4381 ggttcataat tggtcttgat attgactaaa tttgtgcgga ttatagtgga ttaacaaaaa
     4441 ccagtacggc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
     4501 caagtgaatc ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt
     4561 ttgttaatcc actatatagt cataatcggg actgatttta agcgtggggc gataaaaatg
     4621 ccgtctgaaa atatttcaga cggcatattg tttggaaagg gaagtcagtg ttctgccagt
     4681 ttcaggtaaa cgccggaaac gtcctgttcg ccgtagccgg cttcgactgc tttgcggtag
     4741 ctggcagcaa cggtttcgac ggcgggcagg gtgttgcctg cctgttcaag ctctttgacg
     4801 gcgaggttga ggtctttgga ggcgtgtttg agggcgaagg cgggtgggaa ttcgcggttt
     4861 gcccacaggg attttttggt ttggaacatg ggcgagtcca ttgccgagcc gccgatggct
     4921 tcgacgatgg tgtcggtatc gatgccgaac tgccgcgcca tcagcatcgc ttcgctgtac
     4981 gcttcgccga aaatgcccaa gagcgagttc aagacgagtt tcgcgcccga acctttgccg
     5041 acatcgccga aatggaaggt ttttttgccg acgagggaaa atattttttg cagcgggttt
     5101 aaaacggctt cgctgccgcc gaacagaatc agcagcgtgc cgttggtggc gggcccgacc
     5161 gatccggaaa cgggtgcttc ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg
     5221 gcgaggtttt cggtcgggga gatggtgctc atgttgacga tgattttgcc ggccaatccg
     5281 tcgcggactc cgttcaggat gtcgcacacg gcggcatagt cggaaaccat caggaaaatg
     5341 acgggatagt cgcggacgag ttcggcggtg ttgccgtaaa cttttgcgcc tttggcggag
     5401 atgggggcag ttttgtcggg cgagcggttg tatacgccga cttcgatgcc gccgtccaag
     5461 agccgcgtta ccataggcag acccatttgc cctaagccta tccagcctat ttgtgtgtat
     5521 tcgtttgcgg acatgatgtt tactcctttt ctgtttgtag tgtagtgtaa tcttatctgt
     5581 tccgataccg tctgaaaagc ctgcgggtgt ttcagacggt atggtttttt atgcggtcag
     5641 cgtgcctttg gtcgaggggg tctgtcctgc catgcgcggg tcgtgttcga ctgccatacg
     5701 cagggcgcgc ccgaaggctt tgaatacggt ttccgcctga tggtgggcgt ttttgccgct
     5761 gaggttgtcg atgtgcaggg tcatcatact gtggttgacg atgccgtgga aaaattcttc
     5821 aaacaaatcg acatcgaaac gtccgattag tgcgcgggta aattcgatgt tgtacacgag
     5881 tccggggcgg ccggaaaggt cgatgacgac gcggctgagg gcttcgtcga gcgggacgta
     5941 ggaatgtccg taacggcgga tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc
     6001 gagtgtgatg ccgatgtctt cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca
     6061 gctgatgtcg atgtcaatca tgccgtggcg ggcgatttga tcgatcatgt gttcgaggaa
     6121 gggtacgccg gtatccagcc tgcttttgcc gctgccgtcg aggttgatgg agacggtgat
     6181 ttgggtttcg caggtattgc ggttgacggt aacgctgcgg cagccggcag cagatgcggt
     6241 ttcggcaatt tgggtttcgg cagttccggt gctttctgcg gcggtttcgg ggacagtgcg
     6301 ttcgcggtgt tttctgtcga gccagccttt gggtttgccg gtgtgttttt cgagttttgc
     6361 catcaggctg ggacggatgc cgcgtgcatc tgggtcttct gcctgctttt caaggcgttg
     6421 tttgagtttg tagagtgcga cgggggtacg gtagccgcag agtttggcga gcttggacag
     6481 cgaacctgct tcttgggcga gggtcagaaa gttgtgcagt tggcgttgtg ttttagtcaa
     6541 attcatagga aatccgttgg ttggcagtaa agttggcgaa tgatgttgag aacggcatcg
     6601 ttttgtgcgg ggctgcctac ggtaatgcgc aggcagtgtt ccaaaagcgg gtgcgcgcca
     6661 tgcagttttt taaccaagat gcggttttgt ttgagcgtgt caaacaacaa atcggcatcg
     6721 ggtacgcgta tggtaatgaa gtttgcctga cttggaaagg tgttcagacg gcatattttg
     6781 cccaattcgg cgaacatccg ttcgcgttcg tttttcaggc tgtcgatgtt ggcagagatg
     6841 atgccgtagt gttgcagggc gagtttggcg gtggtcaggc tcagttggtt catattgtag
     6901 ggcggcagga ttttttgcag ttcgccgatg acttcgggac agcctgccgc atagccgata
     6961 cgcagtccgg caaaaccgat tttgctgacg gtacgcatga cgatgaggtt gggaatgctg
     7021 cccgcctgcg gcaggaagct gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg
     7081 atgccgtctg aagcttcgat gacggcttcg atttcggcac gcgtgaaaca tacgccggtg
     7141 gggttgttgg ggtaggcgat aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg
     7201 acggcgggca ggttgagggt gaaatctccg ttcagtggaa cgccgacata atccatgccg
     7261 tacagcgcgg cgttgtggcg gtacatgacg aaactgggtt cggctgccaa cattgccgcg
     7321 cccggtttgg cggtcagcat cgtgatgaac tgtatcagct cgtccgagcc gttgcccagc
     7381 gcgatgtcgg cgcagtcggg aatgtcgaac gccgaacgta atgcttcctg taaaccgctg
     7441 ccggagggat tggggtaaag atggatgggc gcggcggcaa gccgtgcctg ccattcctgc
     7501 aacagcgttt catgtccggc aaaagggtgg acgggacttt ccatcgaatc gagtttggca
     7561 aagccgggcg gaacgtcggc aatctgatat gccgacatag cttgtatgtc gtcgcggatg
     7621 aaggagcgga cggatttcat cgtgtttcct taatggttgg aatatgcctg tacgccgttt
     7681 cggcattatt tcatacggaa ctctgccgcg cgggcgtggg cggtcaggct ttcgccgtgt
     7741 gccagcacgc tggcggtttc gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg
     7801 ctggagcgtt tttggaaatc atatgtcccc aaaggcgagg aaaagcgggc agtgcggctg
     7861 gtgggcaata catggtttgg accggcgcag tagtcgccga ggctttcgcc ggtgtagcgt
     7921 cccatgaaaa tcgcgccggc gtggcggatt tttttcgccc attcctgcgg attttcgact
     7981 gacaattcca agtgttcggg ggaaatgtag ttggcgattt cgcaggcttc gtccaagtct
     8041 ttggcgagta tcatcgcgcc cctgttgccg agcgaggctt cgatgatgtc gcggcgcggc
     8101 atggtttcga tgagcctgtc catagcggct tctacttcgt cgagatacgc ttgcgacgtg
     8161 ccgattaaga tggcttgggc aatttcgtcg tgttcggcct ggctgaacaa atccatcgcc
     8221 acccaatcgg caggtgtcgt gccgtcggcg atgaccagga tttcagacgg ccccgccacc
     8281 atgtcgatgc cgaccacgcc gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgccc
     8341 ggaccggtga ttttatcgac ttgggggatg gtttccgtgc cgtaggcgag ggcggcaacc
     8401 gcctgcgcgc cgccgacggt gaagactttg gttacgccgg cgacgtatgc ggcggcaagc
     8461 acgatgtcgt tgcgttcgcc tttcggtgtc ggcacgacca tgatgatttc tttcacgcct
     8521 gcgacgtggg cgggcatggc gttcatgatg acggaactcg gatatgccgc cttgccgccg
     8581 gggacgtaaa tgccgacgcg gtcaagcggg gtaatctgtt gtcccaacag cgtgccgtct
     8641 tcatcggtgt agctccacga ttccattttt tggcgttggt ggtagctttc gacacggcgg
     8701 gcggcggtct gcaatgccgt ctgaacgtcg ttcggaatgc gctcgaacgc cgcgttcaaa
     8761 tcggcttgcg tgagtattaa atcatcgatg cttttagcgt ttgtctgatc gaatttgttg
     8821 gtgtattcaa tcaaagccgc atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg
     8881 atgcgttcgg tttcggggtt ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct
     8941 tggaaatcgg gcgattgggt gttgagtttt ttcatgattt ggttttttcc ttttttattt
     9001 ttggattggg ggtcgtctga atattctctt gttcagtttc tgtctctgtt ttgggtctga
     9061 tatacgcttt ttctcccccg ctaccaaact tcaattcggg caggcatttt tgttgcagac
     9121 cgagtttggc taatacctct tcgctacgag aggtagtgcg gaaacggtaa cgggattgtt
     9181 ttctaccgtt tttggtttcc tcaaccattg tttcaaaacc gtccacttcg tatgcactga
     9241 caacagcgtt ttgcagaccg gtgtgaacgc cgataacgta tttcactttt gaagcaacat
     9301 ctttaccgat aacccagttg cccaacgtac gcgatttgag gttggcatcg tcttggttgt
     9361 cgaaaaggta gtctaattct tcgtcagtat ctaaatcgaa agcattgtgg attttgatgg
     9421 cgagaatcag cccgtcgggg ttaatctcgt taagtgggag agaagagaat ccaaagcggc
     9481 gatctagttc ttctacgcta ataccacccg gaccatgccc ggcaatttta tttttgagtt
     9541 ttttacccaa gacagattta acaaaatgaa ttaaggcaga ttcggcggcg agtgcttcga
     9601 cttcagtcag atgatagctg atgatatagc gaccgagttt cttgcatttg gagatggctt
     9661 tgagtttccg atcgataatc tcgccggaga ctgggtcttg tgaacgacta gcaacccatt
     9721 catgctcgaa gatacgatta ccgcagcctt tgccgatgta gaggattttg tcttttttca
     9781 ggtcggtcag acaatagaca taaaaccgac gctcgccgtt attcaaaacg gaaagggttg
     9841 aatctgagaa tttttttatt tttgcaacca ttttaacttc tcctatacag tttactgccg
     9901 gtgatatttt cagacgacct gtcggcggga acggataggg tcgtctgaaa acgcatcttt
     9961 attcaaatgg atgcttactt cgccttcact gcgccgccga acgcctgaat aatcggctcc
    10021 agcagcgcgt atttcgtttt caaagcagcc ttgttgacca ccaggcggct ggaaatgtcg
    10081 acgatgtgtt cgactgcttc caagccgttt gccttcaagg tgttgcccgt cgaaaccaag
    10141 tccacaatcg catcgctcaa gccgaccagc ggcgcaagtt ccatcgagcc gtacagtttg
    10201 ataatgtcca catggacacc cttgccggca aaatgagatg ccgcgatttc aggatacttt
    10261 gtggcaatct tcagacggca tccgggttgc gaagctgctt cgtaatcaaa ccctttacgc
    10321 acagcaacca tcatgcggca cttggcaatc tccaaatcca aaggccggta aagccccgtg
    10381 ccgccgtgtt cgatcagcac gtctttgccc gcaatgccga agtccgccgc gccgtagcgg
    10441 acataagtcg gcacatcggt tgcgcggaca atgacaaggc ggatgttttc atggttcgtc
    10501 ccgataatca gcttgcgcga tttttcaggc tcttcagtcg gaacaatgcc ggcagcggca
    10561 agcagcggca gcgtctcctc aaaaatgcgc cccttggata aggcgatggt caaagcatta
    10621 tcctgcatgg tgtggatgtc ctttgtcggt tgaaacaata atgccgtctg aataggcgcc
    10681 gacggtgcag acggcatacg gttttatcag agcagagtcc ttacatcggc agcaatcgtt
    10741 tccggctcgc ttccgtaagg cgagaaaatg gcaacctcac cgtttttgtc gatgagatac
    10801 gcaccggaag agtggtcgac caaatagttt tcgctgtcgt ctttttgatt gactttggca
    10861 gaaaccacgc ggtattgctg cttgatgacc ggcaggtttt ggtcgcccgt tgccgtcaga
    10921 ccgataaagt ccggattgaa ctgtttggca tacttgccga tgatttcagg cgtgtcgcgt
    10981 tccggatcga tgctgacgaa caccactttc acgtccttag cctgcccgcc caactgcttc
    11041 aacgtgtcgc tgtacgtcaa aagctctgtc gggcagacat cgggacagtg cgtaaagccg
    11101 aaagacagaa tcacgacctt gcctttcaaa tcgctcaggc tgaaaggctt gccttcgccg
    11161 tcggtcagcg tgaaatcgcc gccgatgtct tccttacgca tatccgtacc gcgcgtttgc
    11221 ggctttgccg cattttccgc agctgacgcg gatgcacttg aagaagcggc ttgcgccgca
    11281 ctgttgtctt gaggtttgca ggcggcaagc gcggcaagta cgaaaacgcc cggcaaaaag
    11341 gaacgcggta cggaaaacat gacaatctcc caagaataaa tagcaaaacc tgattcgtac
    11401 ggaatttgaa tatttccatc ttaacgcgtt tggcagattt aatgtagtat ttttttacgt
    11461 tgtctgcacg gcaggaattg ccgaatcata cagaatcttt ggagcggaaa gaacagtaag
    11521 attaaaataa taaaaattct atttaaaatc aaaaataaaa taaaaacaga taaaataatt
    11581 tcaaaaaaag gtttacagaa tttcaaaagc cattataatg acgacttctt caccagccca
    11641 ggtggcgaaa ttggtagacg caggggactc aaaatccccc gccgcaaggt gtgtcggttc
    11701 gagtccgacc ctgggcacca caaccgcttt tagagcggtt atttttttgt ctattgctgt
    11761 ttaatgaaat agata
//
LOCUS       DAWFJW010000063        11521 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFJW010000063 DAWFJW010000000
VERSION     DAWFJW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.063"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(351..1394)
                     /locus_tag="WGR19_001340"
     CDS             complement(351..1394)
                     /locus_tag="WGR19_001340"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0798859.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDALRLLEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSNPETVLRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1391..1672)
                     /locus_tag="WGR19_001341"
     CDS             complement(1391..1672)
                     /locus_tag="WGR19_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798860.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            2034..2354
                     /locus_tag="WGR19_001342"
     CDS             2034..2354
                     /locus_tag="WGR19_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ0798861.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASWKDINDI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLLYNH
                     IIVD"
     gene            2373..3182
                     /locus_tag="WGR19_001343"
     CDS             2373..3182
                     /locus_tag="WGR19_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0798862.1"
                     /translation="MPRLAGAHTIVSGYLAFGSDESYKNHWDIRDVYAIQLFGKKRWQ
                     LTAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHL
                     AVGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYE
                     AFSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKIS
                     VDELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3411..3764
                     /locus_tag="WGR19_001344"
     CDS             3411..3764
                     /locus_tag="WGR19_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798863.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3825..4451
                     /locus_tag="WGR19_001345"
                     /pseudo
     CDS             3825..4451
                     /locus_tag="WGR19_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
     gene            4403..4603
                     /locus_tag="WGR19_001346"
     CDS             4403..4603
                     /locus_tag="WGR19_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798864.1"
                     /translation="MVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADI
                     LHEKRRLYEWVLEPIYSISGKL"
     gene            4931..5233
                     /locus_tag="WGR19_001347"
     CDS             4931..5233
                     /locus_tag="WGR19_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798865.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5517..5756
                     /locus_tag="WGR19_001348"
     CDS             5517..5756
                     /locus_tag="WGR19_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0798866.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            5817..7559
                     /gene="hrpB"
                     /locus_tag="WGR19_001349"
     CDS             5817..7559
                     /gene="hrpB"
                     /locus_tag="WGR19_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0798867.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKRLAGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7668..10619
                     /locus_tag="WGR19_001350"
                     /pseudo
     CDS             7668..10619
                     /locus_tag="WGR19_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
BASE COUNT         3296 a         2587 c         2799 g         2839 t
ORIGIN      
        1 aaaaaccacg gtgttatcgg caatgactga aaccggagaa atgacgaaat tgaagcgtgc
       61 ggaaatgtgt cggaaatcgc tgaagccgga taaactatag tggattaaat ttaaatcagg
      121 acaaggcgac gaagccgcag acagtacaga tagtacggca aggcgaggca acgctgtact
      181 ggtttaaatt taatccacta tagattcccg cttttgcggg aatgacggta ttttagattt
      241 ttgttttcgc ggaataatga gccaaaagcc gtccgaaccc aaaaaaaccg ccgctttcag
      301 acggcatcaa aaaaccgcag gcacgatgcc tgcggtttgc cgtttgcgtt tcaggggagc
      361 agggggattg cgtccaagcc gtgtgtttcc ttcagtccga acatgatgtt catattttgc
      421 accgcctgtc ccgccgctcc tttgacgagg ttgtcgatga cggaaaggac gacccacaca
      481 tcggattgcg ccgcctgttg gatgctgatg cggcagaggt ttgcgccgcg cacgctgcgg
      541 gtttcgggtg tcgaaccggc gggcaggatg tccacgaaca ggctgtcgcg ataatagtcg
      601 cgcaaaacgg tttcggggtt gctgccgtct gaaaggtgga ggtaaacggt ggcgtgcata
      661 ccgcgtatca tcggcgcgag gtgcggcgtg aacacgaatc cttcggcgat gccgtcctga
      721 agcccggcga tggtctgcct gatttcgggc aggtggcggt gtccggctat gccgtaggct
      781 ttgaagttgt cgccggcttc gcacaacagc gaaccgacat tgcctttcct gcccgcgccg
      841 gatacgccgg atttgcagtc ggcaatcagc ggcataccgg gcttcagacg gcattgccgc
      901 aacagcggca cgagcggtag ggatacgcag gtcgggtagc agccggggtt ggcgacgagg
      961 cgcgcctgtg cgacggcttc gcggttgagt tcgctcaatc cgtacacggc ttgggaaacg
     1021 aggccggggg cggcgtgggt catgccgtac cagtgttccc aggtcggaat gtcccgtatg
     1081 cggaagtcgg cggaaaggtc gatgacgcgc acgccctgtt ccaaaaggcg cagcgcgtct
     1141 ttcatagcga tgccgttggg cgtggcgaag aagacgatgt cgcattgttc caaacctgcc
     1201 tcgtcgggcg tttggaaggc gaggccgtac acgccgcgca gacttggaaa gtaatcggca
     1261 actgcggttc ccgcttcgct gcggctggtt acggcggcga cttcgacatc gggatgggcg
     1321 gcaagcaggc gcagcagttc cacgccggtg tagcccgtcg cgccgacaat gccgactttg
     1381 atttttttac tcatggtttt tcctttgtgt ggttggcggg tatgccgtcc gaacgctgtc
     1441 cgacggggtt ttgattggcg gcggaaggac ggtacacaaa gcgtttgaaa agcgtgtcta
     1501 atttaatggc atccgcctct gtttttaaaa cccagccctg ccgtccggaa taaacataac
     1561 cgtaggcgcg aaagtcggca ggatatttcg gtgcctgccc caaacccaaa ccgtatgcgg
     1621 cgaaagcggt ggtcgtggag aaggaaagca ggaggaggac gagggttttc ataaggttcc
     1681 aaaccgaacg gggcgatgaa gcccgattat agcaaaaggt cgtctgaaaa ccgttttcag
     1741 acgacctttt atttaattac aataaaaatg ttaacaacaa aaaacaaacc gctttttttc
     1801 gtttggaaac aaaacaaatt tttatagtgg attaaattta aaccagtaca gcgttgcctc
     1861 gccttgccgt actatttgta ctgtctgcgg tttcgtcgcc ttttcctgat ttttgttaat
     1921 ccactataac aaaattggca acaattcctt gacgcacaca caaaaaaaac tttaatattt
     1981 gtaaatatat gtaacattat aaattttaaa tattttctga gtaaggggaa gtaatgagca
     2041 tccatctcga cttcggcatt agtcctaaaa cgttccgaca gacttatctg tatcaaaagc
     2101 ccaagctctt taaaggagcg gttcggaatc tcgaagccgc atcttggaaa gatatcaacg
     2161 atatatacca acgagcagac ccaaccgcac cgctgtttca tctgcgtaaa aaaggtgcaa
     2221 ttgttcctaa agaagaatac gtcgaaagtt tcgacgattt gggcaaaact cgctaccgtt
     2281 ttattaaatc cgttatctac gaacatatga agaatggcgc gtcgttgctc tataaccata
     2341 ttatagtgga ttaaatttaa accagtacag cgttgcctcg ccttgccggc gcacatacca
     2401 ttgttagtgg gtatcttgct tttggcagcg acgaatctta taaaaaccat tgggacatcc
     2461 gagatgtgta tgccatccag cttttcggca agaaacgttg gcaacttact gcccctgatt
     2521 tccctatgcc attgtatatg caacagacta aagatactga tatttccatt cctgaacata
     2581 tcgatatgga tattatcctt gaagcaggtg atgtcctcta catcccgcgc ggttggtggc
     2641 acagacctat cccgctcggc tgtgaaacct tccacctcgc tgtcggtacc tttccgccca
     2701 acggctataa ttacctcgag tggctaatga agaaattccc cacgatagaa agtctgcgcc
     2761 acagtttctc agactgggag caagatagga cgcgtatcaa cgatactgcc gcacaaattg
     2821 ctgccatgat tgccgacccc gtcaattatg aagccttcag tgaagacttt ctcggcaaag
     2881 aacgtaccga taccgctttt catctcgaac agttcgcgaa tcccaacgct actccgcttt
     2941 cagacgacgt caggttgaga ttaaatgcca ataatttgga tacgttggaa aagggatatt
     3001 tgattgggaa tgggatgaag ataagcgtag atgagttggg gaaaaaagtt ttagaacaca
     3061 tcggtaagaa tgaaccgtta ttgttgaaaa atctactggt taacttcaat cagggaaaat
     3121 atgaagaagt taggaagttg atttatcagt tgatagagtt agattttctg gaaattttgt
     3181 gagggattct atgaaaaact ggaagcaatt tacatttttc gtaatattag taatagcttg
     3241 ttatcaagtg ctatattttt tatcagatat gtttcttctc gactacatta acaaatatag
     3301 ttggaatttg aattttattc agggtacttt gaattttttt tcaatatatc tgccatatgt
     3361 ttttgttagc cgaattttta ggaataccaa ccaagaaaag gagtataaaa atgattgaac
     3421 ttcaacttca tgaattgaag ctggtttcag ggggaggtcc tgtaacagac aatatagctg
     3481 gaaatgtagc taatgctgcc acaaccaaag gaggtcccac atggggggat ttggttgcaa
     3541 tacctgctgc cgcagcggca gtttattatc tgccgaaaaa tgcttacggt gctgctggtg
     3601 caaatggtgt atacaatgtg actcgtgatt gggtaaatga tgccgttaat gcacctcctt
     3661 ataacggaag accaatcttt gagattgaac atggattaac tgctcccgca acaaaagcag
     3721 ataaatcagg aaacggctac actgacggta cagattactg ctgatatttc tcattgttca
     3781 gacgatctct aggggtcgtc tgaaactttc ttaacttcaa ttttatgaat agacccaagc
     3841 aacccttctt ccgtcccgaa gtcgccgttg cccgccaaac cagcctgacg ggtaaagtga
     3901 ttctgacacg accgttgtca ttttccctat ggacgacatt tgcatcgata tctgcgttat
     3961 tgattatcct gtttttgata tttggtaact atacgcgaaa gacaacagcg gagggacaaa
     4021 ttttacctgc atcaggcgta atcagggtgt atgcaccgga tacggggaca attacagcga
     4081 aattcgtgga agatggagaa aaggttaagg ctggcgacaa gctatttgcg ctttcgacct
     4141 cacgtttcgg cgcaggaggt agcgtgcagc agcagttgaa aacggaggca gttttgaaga
     4201 aaacgttggc agaacaggaa ctgggtcgtc tgaagctgat acacgggaat gaaacgcgca
     4261 gccttaaagc aactgtcgaa cgtttggaaa accagaaact ccatatttcg caacagatag
     4321 acggtcagaa aaggcgcatt agacttgcgg aagatcagta gcaaaaacgg cactgggcag
     4381 acaggaattg tcgggattgg gcatggtatc ctccgatttg gcgaagagca acgaacctgt
     4441 ttatcttgtg aaaataaaac ccgacaaacc aaccatcact gcatacggtg aggaaaaacc
     4501 gctgcaaatc ggcatgacgt tggaagcaga catcctgcac gagaaacggc gattgtatga
     4561 atgggtattg gagccgattt atagtatatc gggcaaactg taagacttga ggctgaaaga
     4621 taaagaagtt gtctgaaacc ttttcagacg accagaagac aatatataaa aacacaaact
     4681 tcaaaactgc ctgaaaccat ccccttttac aggcactccg tctacaggca ctccgtctgt
     4741 aaaagcaagg atagggcatc tgcccttttg attgtttgat taacgataca aggagtttca
     4801 aaatgagagt tttatagtgg attaacaaaa accagtacag cgttgcctcg ccttgccgta
     4861 ccatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactatatg
     4921 tgttcatgaa atgacttggg tcggaggctc aggtaatgcg caacaaagtt catattattg
     4981 cgaaatttgc gaatctgcag ggcttaacga tacgggaaat cctgataaat ctttaggatt
     5041 gccaaacaat acgttcagta atccgcctgg ttggggagct acaatcggag ctttagcagg
     5101 tagccgcata gggatgcctg aatttggtac gtttgcgagc catgccattg aaaatttcga
     5161 ctggtcatgg tatcgacgtt atagggaaat tgccgaaacg attgaacgag aatattcagg
     5221 cggtttgcct taatagttga ggaggtcatg atgtttgcca aacattatca attcatcgca
     5281 ctcggcatca tgctgcttct ttatatgttg attctctata cgaccgattt ttccaatctg
     5341 acgtattgga tgctgttttt tatctgtttt attacaggaa aaatattagc tcgtttgtta
     5401 gagaaaagct ttaaataaaa tagcagctag tcgcaaaagg tcgtctgaaa ccttttcagg
     5461 cggcctttct aaaatacatc caacttccta atccctattt ttcaaaaagg aaatctatgc
     5521 cccatctgca aaacctgtct ttgggcttaa agaaaaagct gcctgttatc ctgcaaacag
     5581 aaatatcaga atgcggcttg gcatgtctgg cggctgtggc gggatttcat ggtttccata
     5641 cgaatttacg cgcactgcgt tcaaaatact gtccgagacc tttgcaaaat tccccaaaat
     5701 cccctaaatg tcttggtggg aattttgggg aattttgcaa aggtctcatt ctataactgt
     5761 aaatactttt aaatttatga caaaatagta aatattgcta aaataatatt gatgtcatga
     5821 aattttttcc tgctccatgt ctgttggtta tcctggctgt catacccctt aaaaccttag
     5881 ctgccgatga aaacgatgca gaacttatcc gttccatgca gcgtcagcag cacatagatg
     5941 ctgaattgtt aactgatgca aatgtccgtt tcgagcaacc attggagaag aacaattatg
     6001 tcctgagtga agatgaaaca ccgtgtactc gggtaaatta cattagttta gatgataaga
     6061 cggcgcgcaa attttctttt cttccttctg tgctcatgaa agaaacagct tttaaaactg
     6121 ggatgtgttt aggttccaat aatttgagca ggctacaaaa agccgcgcaa cagatactga
     6181 ttgtgcgtgg ctacctcact tcccaagcta ttatccaacc acaaaatatg gattcgggaa
     6241 ttctgaaatt acgggtatca gcaggcgaaa taggggatat ccgctatgaa gaaaaacggg
     6301 atgggaagtc tgccgagggc agtattagtg cattcaataa caaatttccc ttatatagga
     6361 acaaaattct caatcttcgc gatgtagagc agggcttgga aaacctgcgt cgtttgtcga
     6421 gtgttaaaac agatattcag attataccgt ccgaagaaga aggcaaaagc gatttacaga
     6481 tcaaatggca gcagaataaa cccatacggt tcagtatcgg tatagatgat gcgggcggca
     6541 aaacgaccgg caaatatcaa ggaaatgtcg ctttatcgtt cgataaccct ttgggcttaa
     6601 gcgatttgtt ttatgtttca tatggacgcg gtttggcgca caaaacggac ttgactgatg
     6661 ccaccggtac ggaaactgca agcggatcca gaagttacag cgtgcattat tcggtgcccg
     6721 taaaaaaatg gctgttttct tttaatcaca atggacatcg ttaccacgaa gcaaccgaag
     6781 gctattccgt caattacgat tacaacggca aacaatatca gagcagcttg gccgccgagc
     6841 gcatgctttg gcgtaacagg tttcataaaa cttcagtcgg aatgaaatta tggacacgcc
     6901 aaacctataa atacatcgac gatgccgaaa tcgaagtgca acgccgccgc tctgcaggct
     6961 gggaagccga attgcgccac cgtgcttacc tcaaccgctg gcaactggac ggcaagttat
     7021 cttacaaacg gggtaccggt atgcgccaaa gtatgcccgc acctgaagaa aacggcggcg
     7081 gtactattcc aggcacatcc cgtatgaaaa tcattactgc cggtttggac gcagccgccc
     7141 catttatttt aggcaaacag cagtttttct acgcaaccgc cattcaagct caatggaaca
     7201 aaacgccgtt ggttgcccaa gataaattgt caatcggcag ccgctacacc gttcgcggat
     7261 ttgatgggga gcagagtctt ttcggagagc gaggtttcta ctggcagaat actttaactt
     7321 ggtattttca tccgaaccat cagttctatc tcggtgcgga ctatggccgc gtatctggcg
     7381 aaagtgcaca atatgtatcg ggcaagcggc tggcgggtgc agtggtcggc ttcagaggag
     7441 ggcataaagt aggcggtatg tttgcttatg atctgtttgc cggcaagccg cttcataaac
     7501 ccaaaggctt tcagacgacc aacaccgttt acggcttcaa cttgaattac agtttctaac
     7561 ctctgaattt tttactgata tttagacggt ctttccttat cctcagaccg tcaaacttta
     7621 cctacgtact tggcgcgcag tacgttcatc ttcaaaatgg aatagacatg aataaaggtt
     7681 tacatcgcat tatctttagt aaaaagcaca gcaccatggt tgcagtagcc gaaactgcca
     7741 acagccaggg caaaggtaaa caggcaggca gttcggtttc tgtttcactg aaaacttcag
     7801 gcgacctttg cggcaaactc aaaaccaccc ttaaaacctt ggtctgctct ttggtttccc
     7861 tgagtatggt attgcctgcc catgcccaaa ttaccaccga caaatcagca cctaaaaacc
     7921 agcaggtcgt tatccttaaa accaacactg gtgccccctt ggtgaatatc caaactccga
     7981 atggacgcgg attgagccac aaccgctata cgcagtttga tgttgacaac aaaggggcag
     8041 tgttaaacaa cgaccgtaac aataatccgt ttctggtcaa aggcagtgcg caattgattt
     8101 tgaacgaggt acgcggtacg gctagcaaac tcaacggcat cgttaccgta ggcggccaaa
     8161 aggccgacgt gattattgcc aaccccaacg gcattaccgt taatggcggc ggctttaaaa
     8221 atgtcggtcg gggcatctta actaccggtg cgccccaaat cggcaaagac ggtgcactga
     8281 caggatttga tgtgcgtcaa ggcacattga ccgtaggagc agcaggttgg aatgataaag
     8341 gcggagccga ctacaccgag atacttgctc gtgcagttgc tttgcagggg aaattacagg
     8401 gtaaaaacct ggcggtttct accggtcctc agaaagtaga ttacgccagc ggcgaaatca
     8461 gtgcaggaac ggcagcaggt acgaaaccga ccgttgccct cgatactgcc gcattgggtg
     8521 gtatgtacgc cgacagcatt accctgattg ccaatgaaaa aggcgtgggt gtcaaaaatg
     8581 cgggcacact cgaagcggcc aagcaattga tcgttacttc ctccggctgt attgaaaaca
     8641 gcggccgcat cgccaccact gccgacagca ccgaagcttc accgacttat ctctccatcg
     8701 aaaccaccga aaaaggagcg gcaggcacat ttatctccaa tggtggtcgg atcgagagca
     8761 aaggcttatt ggttattgag acgggagaag atatcagctt gcgtaacgga gccgtggtgc
     8821 agaataacgg cagtcgccca gctaccacgg tattaaatgc tggtcataat ttggtgattg
     8881 agagtaaaac taatgtgaac aatgccaaag gctcggctaa tctgtcggcc ggcggtcgta
     8941 ctacgatcaa tgatgctact attcaagcgg gcagttccgt gtacagctcc accaaaggcg
     9001 atactgaatt gggtgaaaat acccgtatta ttgctgaaaa cgtaaccgta ttatctaacg
     9061 gtagtattgg cagtgctgct gtaattgagg ctaaagacac tgcacacatt gaatcgggca
     9121 aaccgctttc tttagaaacc tcgaccgttg cctccaacat ccgtttgaac aacggtaaca
     9181 ttaaaggcgg aaagcaggtg gtactgatgg ctgatggcga cattcaggca aaagcctcca
     9241 atctgaatgc atccggcaat ctgtatattc atgcaggtaa ggatctggat ttgaatgccg
     9301 ataaagactt atcaacacaa agtatcagcc tgagggcaga caacaccgcc ctcatcagta
     9361 gcaacggtaa caccttgact gcagaaaaga atctggatat tcaggcaggt agcttgagtg
     9421 tgcgtcaaag taatctgcaa tccagtggcg gtaatgtgca gatgagcgcg actaaaggca
     9481 acatcagctt gaatcagagt tggataaatg ccagtcagaa tattgatacg gcagcacttc
     9541 aaggcaatat tatttcagac ggtctgactg ctgttgccga agttggacgc gtatctcttc
     9601 ttgccaacgg caatgttgat ttcaacggtc taaatacctt gattgcggaa ggagacatta
     9661 atgctggttc agtcggtaaa ggccgtctga aaatggacaa taccgatatt tatgcttctg
     9721 caggcgatgt gaaactggtt gccggaggtc aattagattt aggcaacggc accgttaacg
     9781 gcggtcatat cagcttagac agcaataaag gcagcatggt ggtgcaggat gtacacctga
     9841 atgcccgcgc atcactgaaa gtggatgcgg atcaaacttt aaccattaat aacagcaagc
     9901 tcaattccgg tcacaatacc cagattaata caaatcatgg tcatatgacg cttaatcagc
     9961 ttgatgctca ttcacgtcgt catatgagta tcagtgcaca gggtaaaggc aaaggaaaag
    10021 acagcggtca aattttacaa aacgaccagc aaaacagtaa aagtactttg gcggcagatg
    10081 gtgtattgtc attgaacagc agcgcattac aggttttgga caatactacc ctgcgcggtg
    10141 gcgcgataaa catcaaagct ggcggaggca tcatcaaacg gggctatatc gattgggaaa
    10201 cccaagatac ggctactatg cgttcagcgg aactgaaacc actgtctggt atgatgtcga
    10261 tagaatcggg cggtgataac cctttaaccg ttgaacccgg taaccgtatc gtttctgcag
    10321 gtgatttggc cgtgaaccac aacggcacat tccaaatcag tgccagagca ggaaataacg
    10381 gcaatccgag tgcgcaaaca gccagcgttt cagctaaagg caatatcggg attgtggcgg
    10441 gagaggtgga tattgatgca gcaaatattg ccgcagggaa agatttggct ttagtggcaa
    10501 ctaaagggaa tatttcacta aacagtatta ggaatacgtt tagtaactat caattgaaaa
    10561 cagataaaca caatatcaca cagcaactta ctgatgtaga gcaggagctt agtaagttaa
    10621 cgagcgatcc taaatatcgt aaagcacagg atcttccaca aatgttgagg cgtaaatata
    10681 agaggcgtga caaagtattc ggtgatagtg aggccagatt acgtggttta cgagcagaga
    10741 taaatgctgc tgatgaggct tgggcagaac tccaatcccc agtaaaagcg ttacttgaac
    10801 gtaaacaatt gttacaacaa gcccttttaa cagtttccca accgggtagt ggtcacgaaa
    10861 accaaggtag cacccttagc ggtcaaaata tcaaactgct cgctgcaggt ggtatccgta
    10921 tccagggctc caaagttgct gccacccaac aggcaaatat ccaagctgca ggatttttac
    10981 ctgcaccagc cgcagaggaa ctgcaagaag gtcgacttca atcagccatt gacatcagcg
    11041 gtgtatttga tacttttgaa tatggtcagc aaggcagcga taaatacggc tatgccattt
    11101 tcagcaggcc atctgaaatt tcaggaaaaa caggtgttac cctctctgca ccaaatgcca
    11161 atgagaacag ccgcatcagt ctgagtgcgg caaacatcga agctgaaaac ggcaaaatca
    11221 aactgcaatc ctacggcgac caatcctatt acgccgggca gggcgaactc tataccttag
    11281 aacgccgcag ctacaaaacc ggcaaatggt acaaccgcaa acacattacc gaagtcaaag
    11341 aacacaaaaa cgccaagccc gacgcagtaa acctcagcgc atcccaaggc atcgacatca
    11401 aatctggtgg caacatcgac gcctacgcca ccgcattcga tgcccccaaa ggcagcatta
    11461 acatcgaagc cgggcggaaa ttgacactct atgccgtaga agagctcaac tacgacaaac
    11521 t
//
LOCUS       DAWFJW010000064        11038 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFJW010000064 DAWFJW010000000
VERSION     DAWFJW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.064"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="WGR19_001351"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="WGR19_001351"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0798868.1"
                     /translation="MTVRIEHDLLGDREIPAKVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGHEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAILKNSFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRSLLLEVNLGATAIGTGVNTPKGYAALVVEKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="WGR19_001352"
     CDS             complement(1612..2487)
                     /locus_tag="WGR19_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0798869.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2489..3211)
                     /locus_tag="WGR19_001353"
     CDS             complement(2489..3211)
                     /locus_tag="WGR19_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0798870.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSRHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WGR19_001354"
     CDS             complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WGR19_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0798871.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3787..4149
                     /locus_tag="WGR19_001355"
     CDS             3787..4149
                     /locus_tag="WGR19_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0798872.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKRDAATA"
     gene            4184..5047
                     /locus_tag="WGR19_001356"
     CDS             4184..5047
                     /locus_tag="WGR19_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0798873.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5145..6104
                     /gene="ttcA"
                     /locus_tag="WGR19_001357"
     CDS             5145..6104
                     /gene="ttcA"
                     /locus_tag="WGR19_001357"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0798874.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6198..9137)
                     /locus_tag="WGR19_001358"
     CDS             complement(6198..9137)
                     /locus_tag="WGR19_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ0798875.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGAPPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENTAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMNYREFLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAV
                     GHSENGY"
     gene            complement(9150..10841)
                     /locus_tag="WGR19_001359"
     CDS             complement(9150..10841)
                     /locus_tag="WGR19_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0798876.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT         2650 a         2949 c         2596 g         2843 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctact gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccaaagtgt attggggcat ccacaccctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgccgccgc
      361 cattgaaaag gcttgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccatc
      421 cgatgtttat caaggtggcg ccggtacttc ggtcaacatg aataccaacg aagtcattgc
      481 caacctcgca ttggaagtct tgggtcacga aaaaggccgc tacgacatcg tcaatccgat
      541 ggaccatgtc aacgccagcc aatccaccaa cgacgcctac cctaccggct tccgccttgc
      601 cgtgtattac agcatcggcg aattgctcga caaactggcc atcttgaaaa actcttttgc
      661 cgcaaaagcc gaagagttta aagatgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccgatg actgccggtc aagagtttca atctttccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcaccc gctccttact gcttgaagtc aacctcggcg caaccgccat
      841 cggtacaggc gtgaatacgc ctaaaggcta tgcggcttta gttgttgaga aactttccga
      901 ggtcagcggt ctgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattgcag gccggttctt ccataatgcc cgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgacacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg ccgcagtcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatctgcg aacgttacgt tttcaactcc atcgggctgg ttacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cccgagaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtacc ttgacttccc cccaataaaa caggcaggcg atagaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaag aacaatgcgc ccgaaagcgt
     1741 acccaaaaca atcacgccga tattcatacc ggcaaaaacc aaggtcggat tatccttcat
     1801 catttggtgc gcggtgatgt aggttacgat attcataaaa ttcaaaccgc ccaagaatat
     1861 gccgccgacc acactctcaa cacgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacaccc gccagcacaa atgcaaccag caggttgccc gcaaatgccg ttccgctttt
     1981 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     2041 tgccgcctgc cgccacgcgc ttcctgattt tttgccaccg ctgtgtttcc aaagcaggca
     2101 gaacagtgcg gcaaatgcga ggcacagccc gattagtttg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggcggctt ccacagattt gcccattatc acgaatacgg acggcagcag
     2281 cacgcccaaa gcggcaaaca gcggccacgt cggcaaaaat gcgccgatat tgccgatatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2761 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2821 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agtatggcgc gaacgtgagg
     2881 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2941 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc
     3001 gtctgaagga cgcgtcgtat tgcatccgtt tcgacaggtc ggaaagcgta tcgtaagccc
     3061 gttggatttc tttgaacttt tcttccgcct tcggattgcc cggattgcgg tcgggatgat
     3121 atttcatcgc cagcttgcgg taggcgcgtt tgatttcgtc cgctcccgcc tgcggcgaca
     3181 cgcccaatac ggcatacaaa tctttgtcca tattgctcct tatactttcc cgtcaaaccg
     3241 ccgcctgtat tgttttcggg ttcaaaatat ttaaatctcc gggacggatg ccgataatga
     3301 acccgcgttt tccgccgttg atatagatgg tttccaaatc catcaccgac tgttcgacgt
     3361 aaatatccaa ctttgtccgg atgccgaacg gcgttgtgcc gccgaccaga tagcccgtcc
     3421 acttgtttgc ctgtacgggc gtggcgggtt cgatgtgttt cgcacccaaa tgccgcgcca
     3481 gattgcgggt tgaaatctgc ttgtcgccgt gcatcaagac aatcagcccc ttaccgtttt
     3541 catcttgcaa aacaatggtt ttaatgacca agtgttcgtc tttgccgaat agtcgggcaa
     3601 actgcgccgt gccgccgtgt tcctcatagg cataggtaaa aggttcaaat tcgatgccgt
     3661 tttcacgcaa aacgcgcacg gcaggtgtta tcggataagt gtgtttactc atttttcgtc
     3721 atgcaaaaca tggaaatcgg tcaagcgcaa tgttaccata tatccccttt atcgaaggaa
     3781 accgatatgg atatccgata tttcggcaca acgccccgct attccgaagc ggtttgcgcg
     3841 ggcggtttga ttttcctctc cggcatggtt cccgaaaacg gtgaaacggc tgccgaacag
     3901 actgccgacg tgcttgccca aatcgaccgc tggctggcgg aatgcggctc ggacaaagcg
     3961 cacgttttgg atgcggtcat ctatctgcgc gatatgggcg actacgcgga aatgaacggc
     4021 gtgtgggacg cttgggttgc cgccggcaga acgcccgccc gcgcctgcgt ggaagcgcgg
     4081 ctcgcccgtc cggagtggtg cgtcgaaatc aaaataaccg ccgtgaagag agacgcggca
     4141 actgcataaa cagatttcct tatattttca gagtaggcaa atgatgaaaa ccttactgcc
     4201 ctttttcggc gtggtgcgcg tcagcggcga agaccgccaa accttcctgc acggacagct
     4261 ttccaacgac atcaaccacc ttcagaccgg gcaggcgtgt tatgcgactt acaacacgcc
     4321 caaaggccgc gtcatcgcca atatgattgt cgtcaaccga ggcggcgatt tgctgctgat
     4381 tatggcgcaa gacctgcttg aagcaacggt caaacgtttg cggatgtttg tgttacgcgc
     4441 caaagccgtt tttgaaatcc ttgaagatta cgccgtcggc gcggaattag aagcatccgc
     4501 cgaaccgctt gccgcgcaag aacccagcct cgcctttacc gccgaatgcg gttcagacgg
     4561 catctgttcc gtcgtcctgc cacatcgggg cattttgcac atcgccccca aaaacgcctt
     4621 gccgccttat gatgccgccg ccgaaaacgc gtggcggctg cacgaaatcc gtagcggtta
     4681 tccgtggata tgcgcggcta ccaaagaaac tgccgttgcc caaatgctca accagcacat
     4741 catcggcggc gtacacttca aaaaaggctg ctatcccggt caggaaatca ttgcccgtgc
     4801 ccagtatcgc ggtcaggtca aacgcggctt ggcggtattg tcgggaaatt cggccgtcga
     4861 agcaggaacc ctgctgacgg cagacggcga agagaccggc atcgtactcg acagcgttca
     4921 agattcggaa aactttaccg cccttgccgt gattaaattc tccgccgcgc aaaaagaact
     4981 gaccgcccca aacggcagta tcttcaaagc ggttcatccc ttctttaaaa ccgaaaatgc
     5041 agaataatcc ctctaatgcc gtctgaacct ttttcagacg gcattttaaa accgcccctg
     5101 ttttcggtta caatgccgca tccccaattt ttcaaaccac cgccatgtcc aaaaaaacca
     5161 aacaagaact ggaaaacaac aaactcagca aacgcctgcg ccacgccgtc ggcgacgcga
     5221 ttaacgattt caacatgatc gagccgggcg acaaaatcat ggtctgcctc tccggcggaa
     5281 aagacagcta tgccctcttg gacatcctgc gccagctcca agccagcgcg ccgattgatt
     5341 tccaactggt tgccgtcaat ctcgaccaaa agcagccggg cttccccgaa gaagtattac
     5401 cgacctatct cgaaagcatc ggcgttccct acaaaattgt tgaagaagac acctactcca
     5461 ccgtcaaacg cgtattagac gaaggcaaaa cgacttgttc gctgtgcagc cgcctgcgcc
     5521 gcggcatcct ctaccgcact gcaaaagaat tgggctgcac caaaatcgcc ttgggacacc
     5581 accgcgacga catcctcgcc accttgtttt taaatatgtt ttacggcggc aaactcaaag
     5641 ccatgccgcc caagctggtg agcgacaacg gcgaacacat cgtcatccgt ccgctggcgt
     5701 atgtaaaaga aaaagactta atcaaatacg ccgaattaaa acaattcccc atcatcccgt
     5761 gcaacctctg cggctcgcaa cccaacctgc aacgccaagt catcggcgat atgctgcgcg
     5821 attgggacaa acgctttccc ggccgcatcg aatcgatgtt ctccgccctg caaaacgtcg
     5881 tcccttccca ccttgccgat accgaactct tcgactttgc cggattggaa cgcggtcaaa
     5941 ccctgaaaca cggcggcgat ttggcgtttg acagcgaaaa aatgcccgaa cgtttttcag
     6001 acggcagcga agaagacgaa agcgaaatca aaatcgcgcc gcaaaaagcc gaacgcaaag
     6061 tcatcaacat tctggcgaac aagccgaaaa cctgcggcgc gtaacccccc gccgtcaaaa
     6121 atgccgtctg aagcccgttt ggcgtttcag acggaatttt aaaagcccga agcagacggc
     6181 tcccaaacat ttgtttctca gtatccgttt tcagaatgcc cgactgcggg catattttgc
     6241 ccgatcagtt cggctatcct ctcgccgtca aactgcgttt tgaacaccac cttgatttct
     6301 ttggaaatct cggcaaacag cttttcggca tgtttgattt tccgctcttc gctttttcgc
     6361 aaatcttccg ccccgtcagt ccctttggct tcaatcacaa agttcagaat ctcgccgctt
     6421 ttggttttca cgatataggc aaaatcaggc gaatacgtgc cgccgccggc aacagggatt
     6481 ttgatggagt ttctcggtat tttggtgaat acggttacgc cttcaatttg gttgttggcg
     6541 acattttcat gttctatatc cgaatcgtag aaaatctcgt cgaagagata gccggcggca
     6601 ggccggtgct ccgtatcttc aaatctgccc aaatctgctt tttttaccgc gcgcggtttg
     6661 ccgtctttat cggtaaattt ggtcggatgg attttgctgc cgacaagccg gtaatccagt
     6721 tcgaatttat ggaaggaatg atgaagcaaa aaccggttga agccgttttt gatttgggcg
     6781 atggtctgca tattcaaaaa atctccaatg ttcagttcgt cgcggacgcg gtaaaacgcc
     6841 tgatgcaaag tctgcatgcg gatttttgcc gtttgtgcca gtttttccag aaactctcgg
     6901 taattcattg tgttgaaacg gataaaatct tcatcttcaa aactgtctat gcggcgggaa
     6961 agcataagcc cgttgttgat ataagcttcg tttaccgccg tgcgtatgcc tgcctgcggg
     7021 aatttggcgg tgttttcacg caaataggcg gtaaataaat cgacaaattc ggcttcatcc
     7081 ttgattttgt actgcaaaac ggctttatgg tgaatcagct cccacaaggc tttgagttct
     7141 tcatatttgc cttcgcgcat gatgatggtg tctttgccct cgtctttggc gttgctgact
     7201 ttgcctttgt ccaaaccttt ggggaaggct tcgggatagg cggcttttaa tttgtcatag
     7261 ccgtcttcgg caaagttttc attgtcgtca atgatgccgt ctgaaaacag ttggtttacc
     7321 aataccagcg gtttgatatc ggggtatttt tgcaatattt tttgtttcag ctcttcggta
     7381 aactttttgg agatttcttc ctgaaaagaa ttgtcgttga tttcgccgac aagctgcttc
     7441 acaaagtctt tttcgctgct atcgacaaaa taattcagtt tgtacggtac atcgcgcacc
     7501 cgcgccatca gctcgtttac cggcaggcgc aggccgcgtc cgacttcttg cagcttggaa
     7561 gtcgtgctgc cgctggaacg cagtttgcaa atctggaaaa cgttgggatt gtcccagcct
     7621 tcgcgcagcg tccatttgga aaaaataaag cggcgcgggt tgtccaaaga cagcagtttt
     7681 tccttatcgt gcaggatttc attgatttcc tgctcgattc tatcgtcgct gtctgtattg
     7741 tctttggaaa aatagccgcc gtggcaggcg gatacatcgt ccaatgtctt ttgcaggtaa
     7801 tcgcggtaaa acgggtcgct ttccgttttc agacggcgtg ccgcttccgc gcgaatccag
     7861 ctttcaaatt tatctttcag gctgcctgaa agctcgttgc cgctgcggta gcccgcgata
     7921 tcgtcaataa aaaacagcgt cagcggcttg attttgggct gtggcgcgcg ttctgccaaa
     7981 agcgcgcgtt ccagcttgaa atgttcggca accgcccgct gcatcatcgc atcctgcacc
     8041 gtttgcgaat aggaataagg gttgatgacg gcacccgttt tcaactccaa gccgttgctt
     8101 aacaccacca cggttttatt cattttgtcg attttcaaat ccgaaatagc cggatggatt
     8161 tgcgccaaat cttcgccttt tgccagtttg aacgtctgct ttttgtcctt ttcgtttaat
     8221 tcaaatttcg cttctttgcc gtccgacgac accagtttta ccgccgcatc catgccgccc
     8281 tgcatttctt cctgaaacac gcgcacgcct ttgaccagcc cgtcgttaaa cgcgtctact
     8341 gccgtcaaac ggtaaagcaa gttgtaatat tcatcgttaa atgttgcacc gtagcgcaaa
     8401 atatattgcg gttttaagcg tttgatattg ccccacgttt tcgcgctatc tcgggtcggg
     8461 aatttatgcg gttcgtccac aatcataaac gggcgcacgg cggccaatgc atcaacggga
     8521 ttgtcaaaca aatccttcaa tgccttgtcg cccgtatcgt tcatggacga cgaattaacc
     8581 atgcccgcgt taatcagcag cacatgaatt tcctttttgt tttccgcttt gacaaattgc
     8641 tcaatcgtta tgggcgcatt ggactttttg cccttattct ttttcgcgct ttccaccaca
     8701 taggttttca ggcgtacgcc ttcataatcg ccgccgaaat cctgttcaaa atgctctgcc
     8761 aaagccttgc tttgcaaaaa ctgctgtgtt cccgccttaa tggacaaagt cggcacgacc
     8821 acgataaatt tgaacacgcc cagccaacgg tgcagctcga acatggtttg tgtgtaggta
     8881 taggttttgc ccgtgcccgt ttccatggaa atatcaagga tattttggtc gtccgaacgg
     8941 tcggggaatc ggccgtctat accgttttgg ctttgcactt tcaggatatt gtttgcgtat
     9001 tgttttgcag caaacaaaag ttcgggattt tcgtctgccg tccgatattt gggcggtgcc
     9061 ccgtcaaaca cgcccaaaac cgccgaaacc gcccgcattt ggtgcggctg gtttttctcg
     9121 taattaaaac cgctcatgaa ttgcctccgt caaaccctga taaccacatt caactcaatc
     9181 tcttttttat tggcataacc gcgaaccgcc tggtcaagtt cgtgctgcat ggcgcttgcc
     9241 atattgctgc cgaatacaat cacgcggttg ggattgaaag ccgcatcgtc gtccagcttg
     9301 cggataaacg ccaacaaatc ggcagaagta aaaccggcat tcatcagata cagccgtttt
     9361 tcgcacagat acgccgtata actgcccaac cgcacaggct caaccggcgt ggtcagtgcc
     9421 gccccgtcat acagcgtcca ggtggtcaga agcgtttgca gctgttcttc gcttaattca
     9481 tcgttaagcg gcaaatccgg ttgttcgggc gaaaaatcct tgtccggatg ctgcctgaaa
     9541 ttgtctgccg tttgaaagat tttgaagccc gaatcgcccg tgtaatcggg atgttcgacg
     9601 cggattttgg cggcggcttt ttctatgcgg gctttggtga tgtcgaagat ggtcgggtag
     9661 cctgctttac gggcttcgga tttttcagcg gttttttcgg gaagctgtac acagatatag
     9721 cggcggttac cgttttgtcc ttcggcgtta agctgcatca cggcgtgggc ggttgtgccg
     9781 ctgcctgcga agaagtctag gattaggtca gtttgatttc ccttgaaagt tgcagaaaga
     9841 actaatcttt tcaataaatc taatggtttt ggtgtttcaa aaggagaata accttttcca
     9901 gtttcaaaca actcttctaa ttcacgaata ccatctttag taagattttt acttatattt
     9961 ccaatattta agatgctatg aaatttggaa aaatcatcgg atacttttct aagttttcca
    10021 ataattgttt tcccataaga cttcggaatt aagatatatt cattattttc aatatcttta
    10081 tcaattttgt ttttaaactc aattcctttt atttcaataa tatcttcgta atgcaagttc
    10141 ccatatttgc caaactgttc gcgaacggca tcttcctgta tccaatataa ttcatcattg
    10201 attacaattt ttttgccgcg tatatctttt ggtcttttta gattgtcaaa atgagtaata
    10261 tctttcgcat aaactaaaac agtttcatgc ccatttacaa catattttgc attaccacca
    10321 ccttctgatg tattaacaat taaatcttta ataaaattcc cttccccaaa cacttcatca
    10381 cacaacaact tcaactgcgc cgcttcgtta tcgtcaatcg agataaaaat cacgccgtcg
    10441 tcctttaaca gttcgcgggc gatatacagg cgcggataca taaaggtcaa ccatgcactg
    10501 tgcgagtttg agcctttgtc ggtgaaatct aaaatccgcg cggcttcgtc ttcgtcaata
    10561 ttggctaagc aggcaagttc ggctggtgtg aatttgcggt cgtcctgata gacaaagccg
    10621 tctgatccgg tgttgtaggg cgggtcgatg taaatcatct tcacgctgtt tgtgtaggcg
    10681 ttttttaagt gtttcaacac ttctagattg tcgccacgaa tcagtaggtt ttggctgcct
    10741 gcattttcgg gtttggcgtt gtgcgtcttg tcttcactta tcagggtttc gggcggcagg
    10801 ttgcgaagca ggcgagcata tgatttgccc agccagttca tttcgtaaaa ttcgcgtccg
    10861 atgtcggtct gtggcgcaat ttcggcttgt aatctgtcga taaggaaatt tccgtctgcg
    10921 tcaaaacagg cgggaaacag ttttttgagc tgttcgagtt gggtagagtt ggcggtaatg
    10981 ccgtctgaag tgtagattgc ctcggtgttc accccggctg tgtcgattag atcagggg
//
LOCUS       DAWFJW010000065        10759 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFJW010000065 DAWFJW010000000
VERSION     DAWFJW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.065"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(191..2599)
                     /locus_tag="WGR19_001360"
     CDS             complement(191..2599)
                     /locus_tag="WGR19_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0798877.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPIAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMEIEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2669..3673
                     /locus_tag="WGR19_001361"
     CDS             2669..3673
                     /locus_tag="WGR19_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0798878.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDFGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLVQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYHDGKDKYRPEIPRFLNYLHRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3730..4428)
                     /locus_tag="WGR19_001362"
     CDS             complement(3730..4428)
                     /locus_tag="WGR19_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0798879.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(4722..4829)
                     /locus_tag="WGR19_001363"
                     /pseudo
     CDS             complement(4722..4829)
                     /locus_tag="WGR19_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4887..6425
                     /gene="murJ"
                     /locus_tag="WGR19_001364"
     CDS             4887..6425
                     /gene="murJ"
                     /locus_tag="WGR19_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0798880.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWTHAGGMQKAARLFILIVIGGCLYFASLAALGFRPRHFK
                     RVES"
     gene            6558..7514
                     /locus_tag="WGR19_001365"
     CDS             6558..7514
                     /locus_tag="WGR19_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEZ0798881.1"
                     /translation="MILTPPDTPLFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTFAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPTPDEVSETV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            7564..8346
                     /gene="trpC"
                     /locus_tag="WGR19_001366"
     CDS             7564..8346
                     /gene="trpC"
                     /locus_tag="WGR19_001366"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0798882.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDL
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRTRGVHTFLIGETFMRAADIEAEVGKLF"
     gene            8411..10711
                     /gene="recQ"
                     /locus_tag="WGR19_001367"
     CDS             8411..10711
                     /gene="recQ"
                     /locus_tag="WGR19_001367"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0798883.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGKQESIVNALAGGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSSTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTADFSHNAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2227 a         3032 c         3051 g         2449 t
ORIGIN      
        1 gatgcggttt ttgccttttg cggtgcggcg tggacatcgg ctgctgccag caatgcggcg
       61 gcaatcatca gggctttgat tttcatcatt ctttccttat cgtttgaatc ctacctttga
      121 tggcgggttt ggggttgtcg gtggaatgcc gtcttgtgcg ggaacggtgc tcctgccggg
      181 tttccgacgg tcagggtcgt ttgttgcgtc cggcggaaag agagtgggcg gggatgtagc
      241 cgggaacggc gacatccatc ctgtctgcgg ggtttctgcc gacactgctg aggtctttca
      301 actgaagtga gaaaaagaca gcgtttttgt aggtgttttc gccggtaacg tagcgttggg
      361 cgtacacgcc cgcgccccag cagccgcaac tgcttttgta ttccgcaccc gccagcacct
      421 ctatcggttt tttggcttca aaaccgtagt tgtaacggac gacggccgac aggttgcgcg
      481 tcagcggcca ttgtgcggac aggtcgagct ggctgagttt gtcgtaaaaa taggaaccgt
      541 cggacttcag gtagattttt tcgttgcgcc cgtatttgta gcgggcgttc agcactttgc
      601 cctgtgcggg acggtagctt gcaccgacgg cgtagttctc ggcgcgtttg tcgttttggt
      661 tgtagtggat gctgctgtcg aggatgaagc ggctgccgat gctgccggag gcaaatgcca
      721 cccagtcgga acggttgcgc ggttttttgc cgacgctgcc gtcaagcatc accgcatcat
      781 ccttgaaata gaatttctga ccgatgccgg cgcggaaacg ctcttccccc gtcgcgccgt
      841 ccaaaatacg gctttgcacg gcggcggaaa ggctgtttgc ggtgttaatc ctgtcgttgc
      901 cgtaatagag gttttcgcga aagagctgcc cgtagccgaa gctgctttcc gacgaatcga
      961 aattgggcag gtcgttttgg gatttggcag gaatatagtt gtagaacagg cgcggctcga
     1021 gggtttgcag gactccgccg ccgaacatcc gcgtattccg ctcaaaagtt gcgccgctgt
     1081 cgatgttgac aatgggcaga gtgcggctga cgcgtcgggc ttcttggctg ccgaagcggt
     1141 tgaggctgta ataggtggcg tgcagtccga gtttgggacg gacgtaaccc cagctgttgc
     1201 tgaaatccca tttgatgtcg ggatagacga cgaggcggct gccgtcttgg cggctgtcgt
     1261 ggctgaagcg ggtaaattgt gcggacacgc cgatttgcgc cctgccggtg tttttgcgcc
     1321 aatcggcgga aaggcgcggc atcagggcat acggtttgtc tttgtagccg ctttggtttg
     1381 ccagcgtctg gtatttcaga accgaaaggc cggcattcag gctgccgccc gccgccctgc
     1441 cgccataatc cagccataca cggcggttga ggttgacgtt gccggcgatt tctttgttgc
     1501 cgtaaaagtc gcggtagtag ccgctgtcgg agacttggtt gaaatcgaca cccgcctgaa
     1561 gcgtgtcgga aatgtcgtgc cgatgctgcc atttcgcctg atagcggtta ttcctgccgc
     1621 tttttttgtc gtgcggcagc caggtcaggt cggactggcc ggcataatcc ggccgcaggt
     1681 agcgtacctg cccgtcaaag accgcgccgc gttcgccgat cacgccgggc gcgaacgtgg
     1741 catcgagatt gggggcaagg ttgaaataat agggaacgga aagggaaacg ccgtccgaac
     1801 cggcggacag tgagggaaca agcaggccgc ttttgcggtt gccgtcaagc gggaagtccg
     1861 cccaaggcgt atagaaaagg ggaacgccgc cgaacacgaa ggcggcgtgt ttggcaacgc
     1921 ctatgccttt ttcccgatcg gcttcgacag aggctgcctt gacataccag ccggcatcgc
     1981 cggcggaaca ggtgttgaat tgggtttccg tcagtttgta atgcccttcg cccaacattt
     2041 cggcggtgcg gctgacgctt tgcagccgcc gtccgccttg ttcgatttcc atgcggacgt
     2101 tgtgcgcttc gccggtctgc tgctcgagat tgtaggtcag ggtttcgccc cgaatcagcg
     2161 taccgtcctg ttggagggcg aaccggtcgc ctgcggtaac ggtgtcgccc gactggtcgt
     2221 aatccgccca atcggtattg agggtcgtcc ggttgcgttc gacgacgacg ttgccttcgg
     2281 cacgcacctg cacctgcgac tgtccttcca ccttgtcggc aacaatgcgc gtatagtctt
     2341 cggggatgga tgcttcgccg ctgccttgga cggcggcttc ggttctctcg gggctgccgc
     2401 tttcgttact gcaaaacagg caggtcgaac cgaggctcag gctggtaggc tgtatgggtt
     2461 cggacacgct ccgaacgctt cctccggcga tcggattgtc cgtttcctcc gccgcaacgg
     2521 catcggcggc ggcgcaatgc gtgccgaagc agaggcccaa tgccagcacc agtggtttga
     2581 gtgaaaataa acgagccaaa atcgcccctc aagtcggttt gccagttaga atagcgttta
     2641 ttgtaacctg aaatgcttta gtactgttat gcaacgacaa atcaaactga aaaattggct
     2701 tcagaccgtt tatcccgaac gggacttcga tctgactttt gcggcggcgg atgctgattt
     2761 ccgccgctat ttccgtgcaa cgttttcaga cggcagcagt gtcgtctgca tggatgcacc
     2821 gcccgacaag atgagtgtcg caccttattt gaaagtgcag aaactgtttg acatggtcaa
     2881 tgtgccgcag gtattgcacg cggacacgga tctggggttt gtggtattga acgactttgg
     2941 caatacgacg tttttgaccg cgatgcttca ggaacagggc gaaacggcgc acaaagccct
     3001 gcttttggag gcaatcggcg agttggtcga attgcagaag gcgagccgtg aaggggtttt
     3061 gcccgaatat gaccgtgaaa cgatgttgcg cgaaatcaac ctgttcccgg aatggtttgt
     3121 cgcaaaagaa ttggggcgcg aattaacatt caaacaacgc caactttggc agcaaaccgt
     3181 cgatacgctg ctgccgcccc tgttggtgca gcccaaagtc tatgtgcacc gcgactttat
     3241 cgtccgcaac ctgatgctga cgcgcggcag gccgggcgtt ttagacttcc aagacgcgct
     3301 ttacggcccg atttcctacg atttggtgtc gctgttgcgc gatgccttta tcgaatggga
     3361 agaagaattt gtcttggact tggttatccg ctactgggaa aaggcgcggg ctgccggctt
     3421 gcccgtcccc gaagcgtttg acgagtttta ccgctggttc gaatggatgg gcgtgcagcg
     3481 gcacttgaag gttgcaggca tcttcgcacg cctgtactac cacgacggca aagacaaata
     3541 ccgtccggaa atcccgcgtt tcttaaacta tctgcaccgc gtatcgcgcc gttatgccga
     3601 actcgccccg ctctacgcgc tcttggtcga actggtcggc gatgaagaac tggaaacggg
     3661 ctttacgttt taaacccaat caaaatgccg tctgaaaacc aagtttcaga cggcattttt
     3721 caaacgggct tactgcgcgg ctttttgttc ttcgcgtact ttgtccgcca aaaggtcgat
     3781 ggtgttcata ccggactccc agtcggcaaa ttcgacttta tatttgccgc cgacgataac
     3841 cgtgggcgta ccgtcgattt gaaaggtttc ggtcagctcc tgcattttgt cggcgcgcgc
     3901 ctggctttcg ggagattcgt aagcggcaag gacttttttg ccgtcaaagg cggtttgttc
     3961 gcccagccat tttttgagga cttccggctc ttgcagcttg attttttggt tgaccatcgc
     4021 atcgaaaata tggctgttcg ccacatcttt gctgtcggcg gcagccatat cgacggcggc
     4081 ggcgaggcgt gcgagcgtca gcatttcttt ctgccagacg acgtgttcgg tacgcaggta
     4141 catatcgtct ttaaaagact tggcgtgttt gcttaaaaca ggttcgaggt gggcgcagtg
     4201 cggacagaaa tagccgaaaa actcaaggac ttcgactttg cctgcctgct gttggggaat
     4261 cgggttggca aggacagtat agttttgccc ttcgaccagc cctgccgggg cggcggctgc
     4321 cgaagcggca ggcgcgctgt cggcggggac gctggtttgg actttgctgt cgcacgcggc
     4381 aagtgcgaac agggcggcaa cgccgagggc gaggtgtttg gatttcatac ggctctccgt
     4441 gatgttggaa acagatgttg gaaatcaatc ggaatccggc tattttattg catttccgcg
     4501 tgttgataca gtttgcctcc ggaaaaggac gttttcgttt ggggaaaccg cttcggacgg
     4561 catcaaaccc gatgccgtct gaagcggttt ctgtcgtaca atacgcgccg tcgccccaga
     4621 cggatacggc tgttggggaa ctaatgatgt tccaacacac aggacggcac ataaagcacc
     4681 gccctatgcg ttgccctggt tcggaagggt tacacccctt tcaaatatag tggattaaat
     4741 ttaaaccagt acagcgttgc ctcgccttgc cgtactattt gtactgtctg cggcttcgtc
     4801 gccttgtcct gatttaaatt taatccacta tcaaagtctg atcctgccgc cctaaagggc
     4861 ggggtttcaa ccgaaaagga aatacgatga atatgctggg agctttggca aaagtcggca
     4921 gcctgacgat ggtgtcgcgc gttttgggat ttgtgcgcga tacggtcatt gcgcgcgcat
     4981 tcggcgcggg catggcgacg gatgcgtttt ttgtcgcgtt caaactgccc aacctgcttc
     5041 gccgcgtgtt tgcggagggg gcgtttgccc aagcgtttgt gccgattttg gcggaatata
     5101 aggaaacgcg ttctaaagag gcgacggagg cttttatccg ccatgtggcg gggatgctgt
     5161 cgtttgtgct ggtcatcgtt accgcgctgg gcatacttgc cgcgccttgg gtgatttatg
     5221 tttccgcacc cggttttgcc aaagatgccg acaaatttca gctctctatc gatttgctgc
     5281 ggattacgtt tccttatatc ttattgattt cactttcctc ttttgtcggc tcggtactca
     5341 attcctatca taaattcagc attcctgcgt ttacgcccac gttcctgaac gtgtcgttta
     5401 tcgtattcgc gctgtttttc gtgccgtatt tcgatcctcc cgttaccgcg ctggcttggg
     5461 cggtttttgt cggcggcatt ttgcaactcg gcttccaact gccctggctg gcgaaactgg
     5521 gttttttgaa actgcccaaa ctgagtttca aagatgcggc ggtcaaccgc gtgatgaaac
     5581 agatggcgcc tgcgattttg ggcgtgagcg tggcgcagat ttctttggtg atcaacacga
     5641 ttttcgcgtc ttatctgcaa tcgggcagcg tttcatggat gtattacgcc gaccgcatga
     5701 tggagctgcc cggcggcgtg ctgggggcgg cactcggtac gattttgctg ccgactttgt
     5761 ccaaacactc ggcaaaccaa gatacggaac agttttccgc cctgctcgac tggggtttgc
     5821 gcctgtgcat gctgctgacg ctgccggcgg cggtcggact ggcggtgttg tcgttcccgc
     5881 tggtggcaac cttgtttatg taccgagaat tcacgctgtt tgacgcgcag atgacgcaac
     5941 acgcgctgat tgcctattct ttcggtttaa tcggtttaat catgattaaa gtgttggcgc
     6001 ccggctttta tgcgcggcaa aacatcaaaa cgcccgtcaa aatcgccatc ttcacgctca
     6061 tctgcacaca gttgatgaac cttgccttta tcggcccact gaaacacgtc ggactttcgc
     6121 ttgccatcgg tctgggcgcg tgtatcaatg ccggattgtt gttttacctg ttgcgcagac
     6181 acggtattta ccaacctggc aagggttggg cagcgttctt agcaaaaatg ctgctctcgc
     6241 tcgccgtgat gggaggcggc ctgtatgccg cccaaatctg gctgccgttc gactggacac
     6301 acgccggcgg aatgcaaaag gccgcccggc tcttcatcct gattgtcatc ggaggctgcc
     6361 tgtatttcgc atcactggct gcactgggct tccgtccgcg ccatttcaaa cgggtggaaa
     6421 gctgaccgat gccgatatcc tttttcggca aacgcttttt ccacgcctat attcgggcgc
     6481 gcatcccccg cgtccgcacc ggccggacgg gctgccgcat catcaatgcc gtctgaaaac
     6541 aagaaaaccg ataccttatg attttgacac cgccggacac gccccttttc ctccgcaacg
     6601 gcaatgccga cacgattgcc gccaaattcc tgcaacgccc cgcacccaca taccgccgcg
     6661 agctgcttcc cgacagcacg ggtaaaacca aagtcgccta cgatttttca gacggcattt
     6721 cgcccgatgc gccgctggtc gtgctgtttc acggtttgga aggaagcagc cgcagccatt
     6781 acgcggtcga actgatgctc gcggtacgca gccgcggttg gcacggcgca gtcgtccatt
     6841 tccgcagctg cggcggcatt gccaacaccg ctccggtgtt ctaccacttg ggcgataccg
     6901 ccgaaatcgc ctttactttg gacacgttcg ccgcgcgtta ccgtgaaata tacgccgtcg
     6961 gcgtatcgct gggcggcaac gcgctggcaa aatatttggg cgaacagggc gaaaacgcgc
     7021 tgccgcaagc cgccgccgtc atctccgcac ccgtcgatgc agaggcggca ggcaaccgct
     7081 tcgacagcgg catcacacgg ctgctctaca cgcgctactt cctccgcaca ctgataccca
     7141 aagcacggtc gctccaaggt tttcagacgg catttgccgc agggtgcaaa acactgggcg
     7201 agtttgacga ccgtttcacc gcaccgctgc acggctttgc cgaccggcac gactactacc
     7261 gccaaacttc ctgcaaaccg ctgctcaaac acgttgccaa accgctgctc ctgctcaatg
     7321 ccgtcaacga ccccttcctg ccgcccgaag cgctgccgac accggacgaa gtgtccgaaa
     7381 ccgttaccct gttccaacct gcacacggtg gtcatgtcgg ctttgtcagc agcaccggcg
     7441 gcaggctgca cctgcaatgg ttgccgcaga ccgtcctgtc ctatttcgac agcttccgca
     7501 caaacaggcg ttaacggttt gatgctaata ttcccccttt cccagacgaa tacggaacac
     7561 gacatgaccg acatcctcaa taaaatcctt gccaccaaag cacaggaagt tgccgcccaa
     7621 aaagccgccg tcaacgccga acacatccgc gcacttgccg cagaagccgc gcccgtccgc
     7681 agcttcatcg atttgatacg cggcaaacac cgcctcaacc tgcccgccgt cattgccgaa
     7741 atcaaaaagg caagcccgag caaaggcctg atccgtccgg acttccgacc ggccgagatt
     7801 gcacgcgcat atgaaaacgc cggagcggca tgtttgtccg tactgaccga cgaaccctat
     7861 ttccaaggtt cgcccgaata cctcaaacag gcgcgcgaag ccgtatcgct gcccgtgctg
     7921 cgcaaagact tcatcatcga cgaatatcag gtttatcagg cgcgcgcatg gggggcggat
     7981 gccgtcctgc tgattgccgc agcactggaa caggaacaat tggaacgctt tgaagcggtg
     8041 gcgcacgaat tgggcatgac cgtcctgctc gagctgcacg atgaaaccga attggaaaaa
     8101 tgccgcaacc tgaccacgcc gttgtggggc gtaaacaacc gcaacctgag gacttttgaa
     8161 gtctccctcg accaaaccct gtcgctgctg cccgcgctgg aaggcaaaac cgtcgttacc
     8221 gaaagcggca ttacgggtaa ggcggatgtg gaatttatgc ggacgcgcgg tgtgcacacc
     8281 ttcctgatcg gcgaaacgtt catgcgtgcc gccgatattg aggcggaagt gggcaaactc
     8341 ttctaaatcc cgatttcaga cggcatattg ccgccgaccg atcaacaccc ttaccgagca
     8401 cggaaccatc atgcaccgac cgaccgccaa acagattttg cacgaagtat tcggttatcc
     8461 cgaatttcgc ggcaagcagg agtctatcgt caatgctttg gccggcggcg agagtttgat
     8521 ggtgctgatg ccgacgggcg ggggcaagtc tttgtgttac cagattccgg cgctgatgcg
     8581 cgaaggcgtg gcagttgtcg tatcgccgct gattgcgttg atgaacgacc aagtggccag
     8641 tctgcatgtg gccgggattg aagcggcggc agtcaacagc agcacatcgg cagatgaggc
     8701 gcgcgagatt gccgacaagc ttgcccaagg ccgtctgaaa ctgctttatg tcgcgccgga
     8761 acgcttggtt accgaccgct ttttgcgttt tctcgaccaa caaaccgtca gcctgtttgc
     8821 cattgacgag gcgcattgcg tcagccaatg gggacacgat ttccgccctg aatatcaaca
     8881 gctcggcatc cttgccgaac gctatccgaa cgtcccgcgc atcgccctga ccgctaccgc
     8941 cgatgccgcc acgcgcgccg acatcaagca ttatctgcac ttggacaatg cgcccgaatt
     9001 tgtctccagc ttcgaccgtc cgaatattta ttatcaggtt atcgaaaaaa acaacggcaa
     9061 aaaacaattg ctggatttca tccgcaaaga aatgacgggg caaagcggca ttgtgtattg
     9121 cctaagccgc aaaaaggtgg aagatgtggc gcagtttttg cgtgaaaacg gattaaacgc
     9181 gattccgtat catgctggtt tgagcatgga cgtgcgcgag gaaaaccaac gccgctttac
     9241 gcatgaagac aatattatcg tggtggcgac cgtggcgttc ggcatgggca tagacaaacc
     9301 cgacgtgcgc tttgtcgccc atctcgatat gccccagagt gtcgaacatt tctatcagga
     9361 atcggggcgt gccggccggg acgggctgcc tgccgtgagc tggctgtgtt acggtttgaa
     9421 cgattgggtg ttgctgcgcg aacggattgc cgaaggcaac agcgacgagg tgcaaaagca
     9481 aatcgaaatg caaaaactcg atgccatgct ttccgtctgc gaaaccgccg cctgccgccg
     9541 cgtactgctg ctcaaacatt tcggcgaagc atccgaaccc tgcggccatt gcgacaactg
     9601 cctgcatccg cccgtacggt ttgacggcac ggtgttggtg caaaaattac tcagctgcgt
     9661 gtaccgcgcc ggacaacgtt ttgccgccgg ttacatcacc aaccttttgc gcggcaaaag
     9721 cgacgattgg atacgcggca accggcacga acaactgtcc accttcggca tcggtacgga
     9781 gttgtccgac aaagaatggc gcagcgtcat ccgccagtgt atcagcctcg gctacctcac
     9841 cgtcaacatt acccgatatc aggcattgca actgaccgaa gccgccaaaa aagtcctcaa
     9901 aggcgaaacc gaagtgatgc tgcgtccgct caagcgcgac aagcccgccg cccgcaccct
     9961 caaagacaac tggctgcgta ccgaacgcga agaacgcctg tggcaggcat tgcgcgtttg
    10021 gcgtatgaaa caggcagaag ccgaagtcat ccccgcctat atgattttcg gcgacaaaac
    10081 cctgcgcgac cttgtcgaaa aaatgccgca agacctcaac ggactgcacg acatctacgg
    10141 tttgggcgaa gccaaaatcg accgtttcgg acacggcatc ctcgaagtct gccgcaacac
    10201 cgccgacttt agccacaatg ccgtcatccg cccgcaaacc gaacgcgaac aacaactgcg
    10261 tcaaaaactc gaagcctggc ggtatgaaca ggcaagggcg gaaaactgcg ccctgcatgc
    10321 cgtcctctcc gacgaaagcc ttgccgatat gcttgccgat acgcccgaaa ccgaaaccga
    10381 cctcgaaggc gtacacggct tgggcagcgt acgcgccgcc aaatacggac gggacatcct
    10441 cgccgtctgc cgtccgtttt cagacggcat cgatgaagcc gccaaacaca aacgccgcct
    10501 gatgcgcgcc ctgattcaat ggtgcaacga aacggcaaaa cacgaacagt ccgaacccta
    10561 ccgcattctc agcaaagccg ccctgcgcgc cattgccgcc aaacagccgg aaggtttggc
    10621 ggagcttgcc gccgtatacg gcgtaggcga agaaaaagcc gcacgttacg gtgcggcggt
    10681 gttggcggta ttggaacgaa atgccgtctg aaaaccgtta ccaagtttca gacggcattg
    10741 cctctattta aaaattcct
//
LOCUS       DAWFJW010000066        10533 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFJW010000066 DAWFJW010000000
VERSION     DAWFJW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.066"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            121..303
                     /locus_tag="WGR19_001368"
     CDS             121..303
                     /locus_tag="WGR19_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798884.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(365..1366)
                     /locus_tag="WGR19_001369"
     CDS             complement(365..1366)
                     /locus_tag="WGR19_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0798885.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1443..5276)
                     /locus_tag="WGR19_001370"
     CDS             complement(1443..5276)
                     /locus_tag="WGR19_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0798886.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHNGVEFVELAGLDGKHPIIHCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGRNIVNTKRLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVANATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKARYGAA
                     VHAAPEAKDNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGTA
                     SIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5537..5633
                     /gene="ffs"
                     /locus_tag="WGR19_001371"
     ncRNA           5537..5633
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGR19_001371"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5801..7492
                     /gene="dld"
                     /locus_tag="WGR19_001372"
     CDS             5801..7492
                     /gene="dld"
                     /locus_tag="WGR19_001372"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0798887.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINDNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFARHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7624..7731
                     /locus_tag="WGR19_001373"
                     /pseudo
     CDS             7624..7731
                     /locus_tag="WGR19_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7787..8158
                     /locus_tag="WGR19_001374"
     CDS             7787..8158
                     /locus_tag="WGR19_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0798888.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8313..8861
                     /locus_tag="WGR19_001375"
     CDS             8313..8861
                     /locus_tag="WGR19_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0798889.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8991..10082
                     /locus_tag="WGR19_001376"
     CDS             8991..10082
                     /locus_tag="WGR19_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0798890.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLAGIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGDGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         2463 a         2920 c         2820 g         2330 t
ORIGIN      
        1 ttgtactgtc ttcggctcgc cgccttgtcc ttatttaaat ttaattcact atatttgccc
       61 gtgcgtttgt gataaattcg gcactgtatt tgatatgatt ttaacaaatc gggaggggca
      121 atgttttggt atgtaatcgg cttttgctcc tttgtcgtcg cgctgctgtc gctgtgggtc
      181 aatgccggcg cattcggtat gcaggaagac gataccccgc aatcggatta tgaaaaacgt
      241 ttgggcttgg gtgcgaagct gaaaaacaaa aatacgccgg atacggaaga aaagcagcaa
      301 taaagatatg aaatgccgtc tgaaagattt tcagacggca tttgctttga agcatgggag
      361 acagtcaaac atttatctca tgctcaaatg cgttcaggat tcggcggact tcatccgcat
      421 ccttaaccgt tcggacgcgg tcaaacagcg tttgcgccgg gtcgaacgtt tttttcatca
      481 tgcccaacca ttgcttaaga cgggcgatcg ggtatttatt gtttgcctct tttgtcaggc
      541 acagctcgaa aaactgccgt atccatttgg aaacttcggc aaaatccgtg tctttgaccg
      601 gcccgccgtt ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accgcgccgc
      661 gaccgagcat cacgctgttg cagccgctga ttgttttgat gccgatatag tcttgcaggc
      721 tgaaaacgtc gccgttggcg gtaacgggaa tattgacgct gtctcggatt ttccttatcc
      781 attcccaatg cgccggcggt tcgtaaccct cggctttggt acgcgcgtgt acggtcagtc
      841 cgcatgcgcc cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt
      901 aaccgagccg catttttgcg gtgagcggaa tatgtgcggg caaacgtccg cgcagcgttt
      961 tgacgatgtg gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt
     1021 tgacggtggg cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg
     1081 cttccagcgc attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg
     1141 gcgtgccgga aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg
     1201 atcgggaatg cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc
     1261 caatacgcgt cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga
     1321 tgattcgggt tttttgtttg ggttcggttg tttgtccgtc aatcatgatt tgggtaggta
     1381 ttgggtttaa acaatgccgt ctgaaggctt tcggcgttca gacgacattt ccgtgtccgt
     1441 tgttacagca atactttttc cacgccgccg ttgttggctt ttttcacaaa ctcgtctagc
     1501 cagttttcgc cgagaatgtg tttcgccatt tcgatgacga tgtagtcggc aggcatattg
     1561 ttgtcgtcgg cgtagcggct caaaccttgc aggcaggcgg ggcaggaagt cagcattttg
     1621 acgggttcgc cctgcggcag ctctttgagg tttttctcga tttcctcttg tttgcggaac
     1681 ttgacctgag tggcgatgtc tggccgtttg acggcaaaca taccggattc gccgcagcag
     1741 cggtcgctta aaacgacttt ctgtcccatc aggctgctgg ccatttgggt ggcgttcatg
     1801 gttttaatcg gggtgtggca ggggtcgtgg tagaggtatt gctgaccttt cacgccgtcg
     1861 agtttcacgc ctttttcgag cagatattcg tggatgtcga tgatgcggca gccggggaag
     1921 atttcttcaa agcggtattt ttcgagctgg tcgtaacaag tgccgcaact gacaacgacg
     1981 gttttgatgt cgaggtagtt gagggtgttc gccatacggt ggaaagccac gcggttgttg
     2041 gtgctcattt cttcggcttt agccttgttg ccgcccgcgt cttgcggata gccgcaacac
     2101 atatagccgg gcggcaggac ggtttgtacg ccgacgtgcc agagcatggc ttgaacggca
     2161 agtccgattt ggctgaacag acgctcagag ccgcaacccg ggaagtagaa cacggcttcg
     2221 gcatcttcgg gcgcggcggg gttgcggatg atggggatgc ttttgccgtc ttcgatgccc
     2281 aataaggagc gcggtgtttt ggcgggtacg cttttcggca aaggacggtt gatgaaatgg
     2341 ataacctgtt ctttgattga ggcagtaccg acggttgcct tgggttcggc tttttgcttt
     2401 ttcgtgccga ttggaagaag tttgccgatt ttgtaggcaa agttctgcgc ggggaagccg
     2461 gtctgtatca tcgcggcgcg aagggctttg atggttttcg ggccggtggc gttcaaaaac
     2521 gccatgccca tcgatgcggc gggtgcaaat cgtttgtggc cggaatcggc aaggtagttg
     2581 cggacggcta cggttacgtc gccgaagtcg atgttgacgg ggcagggttt gacgcagcgg
     2641 tggcacacgg tgcagtggtc gccgatgtcc atgagttctt caaagtgttt gacggaaacg
     2701 ccgcggcggg tttgttcttc gtataagaag gcttcggtta acagccctac gccgaggatt
     2761 ttgttgcgcg gactgtacag caggttggcg cgcggaacgt gggtggagca gacgggtttg
     2821 catttgccgc agcgcaggca gtctttgacg gaatcggcaa tcgtgccgag gtctgatttt
     2881 tccataatca gcgattccgc gcccaaaagc tcgaaagacg gcgtgtaggc gttgcgtaag
     2941 tccgagcctt tcatcagttt gtgacggttg aaggtgtgtt tgggatcgac ttggtttttg
     3001 tagtcccaaa acggctgcat ttcttcgtcg cttaaaaatt cgagcttggt aatgccgatg
     3061 ccgtgttcgc cggaaatcac gccgttaagc gaacgggcga ttttcataat gcgttcgact
     3121 gagcggtatg ccgtctgaag catttcggca tcgtctgagt taaccggaat attggtgtga
     3181 acgttaccgt cgccggcgtg catatgcagg gcgacaaaga cgcggctgcg tacggttttg
     3241 gcgtggattt ttcccaagcc ttggataatt ttggtgtcgg ttttgccgct gaagatttca
     3301 gaaagcggct tcattacgtc cgctttgaca gacacgcgca ggcggaaatc gcggaaggca
     3361 ataaagcaac tttcattgtc tttggcttcg ggcgcggcgt ggacggctgc gccatagcgt
     3421 gctttatagt cggcaagcgg cgcgtccaag ttggcaagca gccattccca acgcgctttg
     3481 acggcggaaa cgtgggcaag ggcgtgtttg ccgcgttcgc ccaacagttc ggcggtcggc
     3541 aggtctgttc ccattttgtc gatggggagt ttgcccgaaa gatattgctc caaggcggca
     3601 cagagtttga gtttgttttg gatggaaagc tcgatgttga tgcgctcgat gccgtctgaa
     3661 tactcgccga gcctttcgag tgggatgacc acgtcttcgt taattttaaa ggcgttggtg
     3721 tgcttggcga tggcggcggt gcggctgcgg tcgagccaga aggttttgcg ggcttcgggc
     3781 gatacggcga taaagccttc gccgtcgcgg gcgcgtgcga gttcgcagat gtgttcggcg
     3841 gctgcctcta cggcggcttc gtcgtctgaa accacgtctg ccagcaaaac cattttcggt
     3901 cgtcccttgc ccgccgcttt ggtggcatag ccgacggcgc ggacataacg ccagtccaaa
     3961 tgttccaaac ccgccaaccg cacactgtcg tgggcgagca ggaaatcgcg gatttcgacg
     4021 atggacggcg tggcattggc gaccgtgccg aaaaactcca tacacacggt gcgcgtgtat
     4081 ttcggcattt tatgcaacac gaaggcaacg ctggtgatga tgccgtctgt accttctttt
     4141 tgcacaccgg gcagtccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg
     4201 cggaacttgt gaccggggat ttccaaacgt ttggtattga cgatattgcg gccgtctgaa
     4261 tccagcgtat gtacgtcaaa cacggcggtt tcttcatcgt ggattttgcc gaaattgtgg
     4321 cgcacgcgct cgatacgcag ccattcgcct tgagggttga ccatctgcca gtaggcaagg
     4381 ttgtccaacg ccgtccccca cagcacggct tttttaccgc ctgcgttcat cgccacattg
     4441 ccgcccacac aggacgcatc ggcggaagtc ggatcgacgg cgaacaccaa acctgcctga
     4501 tgcgcggttt cttccacccg ccgcgttacc acgcccgcgc cgcaatggat aatcggatgt
     4561 ttgccatcca agcccgccag ttcgacaaac tccacgccat tatgtttatc cagcttttcc
     4621 gtattgatga ccgcgctcat tgcgtccaaa ggaatcgcgc cgccggtata acccgtgccg
     4681 ccgccgcgcg ggataatgac caaatccagc tcgattaagg cgcgcaccaa aggcgcgact
     4741 tccgcctccg tgtcgggatt gacgacgaca aacggatatt cgacgcgcca gtcggttgca
     4801 tcggtaacgt gcgttacccg cgccaatccg tcaaacataa tattgtgcgg cttggtgatt
     4861 ttgctcaaac gctccaaaat ctgccgccgt ttttggctgg tttcatcaaa actgctatca
     4921 aaacgctcga ctgctttttc tgctgcggca accaacacat ccacttgccg attatcgtcg
     4981 cggcgtttgc ggatttcatt taagcggtgg cgcatttcac gtaccagcgc ggcgcggcgt
     5041 ttcggatgct ccagcaaatc atcaaccaga tacggattgc gcacgaccac ccaaatatcg
     5101 cccaacactt caaacagcat tcgtgccgaa cgcccggttt tgcgttgacc gcgcaggtct
     5161 tgcaggattt gccacgcttc gtcgcccaat aatcggatga cgatttcgcg gtcggtgtag
     5221 gaagtatagt tgtaggggat ttcccgaata cgctgagggg cggtagtcgt ggtcatggtg
     5281 tgtccgtgtt tggcagtttt tatttgaatg ccgtctgaaa agtcggtttc aggcgagttt
     5341 gaaaaggcga taatgtagtt caatttttga tacttttcaa tgccgaaaca ggaaataccc
     5401 ttatagaaac aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta
     5461 ggcggtgttt ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat
     5521 aatgccgttt ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc
     5581 ggaagccagc agcccactct gatgcgccag tgccgggggt ttggcccgcc gccctatttg
     5641 aaacgccgga gcttcgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg
     5701 ttcagacggc attttcttta ctttgccata acgtataact acaatgtgaa aacaattcca
     5761 atgtgcatat gcacctgccg acaattttaa ggaaactgcc atgagcgcat cacaattact
     5821 gtcaagactg acccaaaccg taggcgaaaa atacatcatc accgaccccg cgaaaaccga
     5881 acaataccgc caaggctacc gcttcggcga gggtaaggcg ttggcggtgg ttcgccccgg
     5941 aagcattctg gaaatgtgga aaattctgca ggcgtgcgtc gaagcagacg tgattgtgat
     6001 tacgcaggcg gcgaataccg gactgaccgg cggttcgacc cccgacggca acgattacga
     6061 ccgcgacatc gtgattgtga acaccatgcg gatgaacatc atccaaacca tcaacgacaa
     6121 cgaacaagtc gtctgcctgc ccggctcgac cctgaaccag cttgaattgc tgctgaaacc
     6181 cttgggacgc gaaccgcatt cggttatcgg ctcttcctgc atcggtgcgt ccgtcttggg
     6241 cggcgtgtgc aacaactccg gcggcgcatt ggtacagcgc ggcccggcct acaccgaaat
     6301 ggcactgttc gcccaaatca acgaagaagg caggttggaa ctggtcaacc acttgggcat
     6361 agacttgggc gacacgcccg aagaaatcct gaccaacctg caaggtcatc attatcaaaa
     6421 caaagacatc aaacaagacg cgggcaaagg gcacgaccac gcctattgcg aacacgtccg
     6481 ccaagtggac gaaccgaccg ccgcgcgttt caatgccgac cccgcccgcc attacgaagc
     6541 ctcgggctgc gcgggcaaac tgatggtttt tgccgtccgt ttggacacct tcccgcaaga
     6601 aaaacaaacc gccgtgttct acatcggcac gaacgacatc aacgagctga ccgacatccg
     6661 ccgcgccgcc ttgggcgaat ttgaaagcct gcctgtttcc ggcgaataca tccaccgcca
     6721 cgctttcgac attgccgacg tgtacggcaa agacacgttt tacgtcatca aaaaattcgg
     6781 tacgcaccaa ctgccgaaat tatttgactt gaaggcgcgc gtggacaggt tcggcaaaaa
     6841 agccagcttc ctgcccaaac atttttccga caaggccatg cagttcgtca gcaaatacct
     6901 gcccgaccac ctgcccaaat ccatgcgcga ttaccgcgac aaatacgaac accacctgat
     6961 tctgaaaatg ggcgggaaag gcgtagatga agcgcgcgcg ttcctgaaag aatattttgc
     7021 gcgccacagc ggcgcgtttt tcgaatgcaa cgccgaagaa acccaagccg cgatgctgca
     7081 ccgtttcgcc gtcgcctccg ccgccatccg ctaccgcgcc gtgcatgacg acgaagtgga
     7141 agacttggtc gcgctcgaca tcgccctgcg ccgcgacgac cgcgactggt ttgaaaaact
     7201 gccgtcggaa atcgacaata aaatcatcca caagctctac tacggacatt ttatgtgcca
     7261 cgttttccat caggattaca tcatcaaaaa aggcaacgac tgtatggcgt tggaacacga
     7321 aatgctgcat cttttagacc aacgcggcgc gcaatatccc gccgaacaca acgtcggtca
     7381 tttgtatgaa gccaaacccg cgctcaaaca gttttaccgc aaacttgacc cgaccaacag
     7441 cttcaacccg ggcgtaggca aaaccagcaa aaagaaaaac tgggcggaat aagcgcgtcc
     7501 gctttgaagg caggcaatgc cgtctgaagg caaaacgccg ttcagacggc attgtcgggt
     7561 ttgaagttcc ccgtcgggta atcgctttca aaccgtatgc cgacacacgg gcgcgcccct
     7621 cccaaatata gtggattaaa tttaaatcag gacaaggcaa cgaagccgca gacagtacag
     7681 atagtacggc aaggcgaggt aacgccgtac tggtttaaat ttaatccact ataaagtctg
     7741 atcctacctt cccaaagggc agggtttcaa ccgaaaagga aacacgatga aaccatacaa
     7801 aatctacacc catcccgccc tgccgcccca agccgtcaaa caaggctggt catggccggg
     7861 gctcttgttc ggcacgctgt gggcgtgttt caaaagaatg tggggtttgg ggctggggct
     7921 gacgggcgcg atattcgtgc tggctgtgtt tgcccaactg gtttacggcg acacgcccgc
     7981 cacagactcg gcgtttaacg tattgggctt ggcggtttcc gtctggttcg gcgcaaaggg
     8041 caacagcctt tatgcacgcc accttttatc gcgcggctat accgaattgc ccgaaacggt
     8101 tgaggcagcc aatccccaag ccgcattggc gcaatatttc gggcgcgggg gccgataagc
     8161 gcgttttctt ttccatcatg ccgtctgaaa aatgttcaga cggcatttct tataaccttt
     8221 tgtttgcagc acataaccaa tcagtctttc ctattccgtt ccgaaatgca taccatgcgc
     8281 ccgtacccaa tatttgcgaa caaggaaaga aaatggcacg tttaaccgta cacaccctcg
     8341 aaaccgcccc cgaagccgcc aaagcgcgcg tcgaggcggt acttcaaaac aacggcttta
     8401 tccccaacct tatcggcgta ttatcaaacg cccccgaagc cttggcgttt taccaagaag
     8461 tcggcaagct caacgccgcc aacagcctga ccgccggcga agtcgaagtc atccagatca
     8521 tcgccgcccg taccaaccaa tgcggttttt gcgtggcagg gcacaccaaa ctcgcaaccc
     8581 tgaaaaaact cctgtccgaa caatccgtca aagccgcgcg cgctttggca gcaggcgaat
     8641 ttgacgatgc caaactcggc gcgctcgccg ccttcaccca agccgtaatg gcgaaaaaag
     8701 gcgcggtatc cgatgacgaa ctcaaagcat ttttcgacgc gggctacaac cagcagcagg
     8761 cagttgaagt cgtgatgggc gtagccttgg caaccctgtg caactacgtc aacaacctcg
     8821 gacaaaccga aatcaacccc gaattgcagg cttacgcctg atacggcaaa ccgcccgaat
     8881 atcgggcggt ttttcaaccc ccctcaaaca aaaacaagcc gccaaacagc agggaagcag
     8941 ccgcgcatca ggcgtgcgcc cgattccccc gcaaacctta aggaacaaag atgaacgccc
     9001 aaaccctgat tgccaacgtt gccgaactcg tcaaaaccaa gctcaaaccc atagtggacg
     9061 acatcgaccg caaaggatac taccccgaag catttatgcg cgaactcggc gcaatcggcg
     9121 gattcggcgc agtcggcaca gaagccgaag gcggcaacgg cttgggtttg gcaacgcaaa
     9181 tcgccgtctt gcgcgaaatc ggcaaagaat gcggcgcaac ctccttcagc gcgtggtgtc
     9241 aggcggcttg cgcgtggtat ctgcaccaaa cgcccaacca agccgtcaaa gacaaatacc
     9301 tcgccgacat cctgcaaggc aaagtattgg cgggtacggg catgtccaat accgtcaaac
     9361 accttgccgg catcgaaaaa cacaacctcc aagctgagcg agtggagggc ggctacacag
     9421 tcaacggcgc gctgccgtgg gtgtccaaca tcggcgaaga ccacatctgg gcgaataccg
     9481 cccaaatcgg cgacggttac gttatgttta tcaccggcgg acaatgggaa ggcgtaagcc
     9541 tgcaaaactg ccccgaattt tgcgccctcg aaggcacgcg caccttcagc ctgaacttca
     9601 aagacgtatt tatccccgac gaagacatca tcgccgcgcc cgaacagttt gccgactaca
     9661 tccaaagcat caaagcaggc tttatcctcc tgcaaatcgg catcggcgcg ggcgtgatag
     9721 acggcagcct cggcatcatc cgcctcgcca acatcgtcaa tgcagaagtc aacagctatc
     9781 tcgacgacgg ctacgacagc ctcaaagcaa ggttggacgg cgcgtgggca gaaaccgaac
     9841 ggctcgccgg cttggcttgg agcggcacgc ccgacaacct tgctaccctc aagctgcgcg
     9901 aagccgccgc cgtactggca cttgccgccg cccaatccgc cgcactgcat tccggcgcaa
     9961 aaggctacct gatgcgcagt cccgcccaaa ggcgcgtccg cgaagcgatg tttgtcgcca
    10021 tcgtaacccc cgcaatcaaa cacctacgca aagaaatagc ggcaatcgaa gccgcaaaat
    10081 aaacaggcgg aaaaaaccga aacccccacc gcgtcattcc cgcgaaagcg ggaatccagc
    10141 ccccaacgca acaggaatcc atcggaaaaa acagaaaccc ccaaaccgtc attcccgcaa
    10201 aagcgggaat ccagccccca acgcaacagg aatccatcgg aaaaaacaga aaccctcaaa
    10261 ccgtcattcc cgcaaaagcg ggaatccagc ccccaacgta acaggaatcc atcggaaaaa
    10321 acagaaaccc tcaaaccgtc attcccgcga aagcgggaat ccagccccct aacgcaacag
    10381 gaatccatcg aaaaaaccga aacctccaaa ccgtcattcc cacggaagtg ggaatccagt
    10441 aaccgaaaaa ccacaggaat ctatcgaaaa aaaccccaac ccccaaaaaa accgggcgga
    10501 tacctcacca tcctcccaaa ccctgattta tag
//
LOCUS       DAWFJW010000067        10307 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFJW010000067 DAWFJW010000000
VERSION     DAWFJW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.067"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(376..1593)
                     /locus_tag="WGR19_001377"
     CDS             complement(376..1593)
                     /locus_tag="WGR19_001377"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0798891.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1795..2523)
                     /gene="rph"
                     /locus_tag="WGR19_001378"
     CDS             complement(1795..2523)
                     /gene="rph"
                     /locus_tag="WGR19_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0798892.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2606..3070)
                     /locus_tag="WGR19_001379"
     CDS             complement(2606..3070)
                     /locus_tag="WGR19_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0798893.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAIAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3287..4063
                     /locus_tag="WGR19_001380"
     CDS             3287..4063
                     /locus_tag="WGR19_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0798894.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     IEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVFSTENIVPPQSGVAY
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4308..5162
                     /locus_tag="WGR19_001381"
     CDS             4308..5162
                     /locus_tag="WGR19_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0798895.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFISLLELAKEGLVRIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5203..6411
                     /gene="pncB"
                     /locus_tag="WGR19_001382"
     CDS             5203..6411
                     /gene="pncB"
                     /locus_tag="WGR19_001382"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0798896.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6503..8221
                     /gene="argS"
                     /locus_tag="WGR19_001383"
     CDS             6503..8221
                     /gene="argS"
                     /locus_tag="WGR19_001383"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0798897.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPIVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGSIKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8768..10186
                     /locus_tag="WGR19_001384"
     CDS             8768..10186
                     /locus_tag="WGR19_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0798898.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2389 a         3018 c         2586 g         2314 t
ORIGIN      
        1 gcgcaggcgg gaatctagac attcaacgct aaggcaattt atcggaaatg actgaaactc
       61 aaaaaactgg attcccactt tcgtgggaat gacgggatgt atagtggatt aacaaaaacc
      121 agtacggcgt tgcctctcct tagctcaaag agaacgatta tctaaggtgc tgaagcacca
      181 agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt
      241 gttaatccac tataaattta atccactata ttttttgttc caaagtcaaa atatgccgtc
      301 cgaacattcg ggcggcagac aaaacggcac tgcccgataa aggcagtgcc gttgtccgtt
      361 tcaaaccgtg aaacatcagc ccaaattaaa ggctttatgc aataccctgg ttgccagttc
      421 catgtatttt tcatcaatca atacggaaac tttgatttcg gaggtggaaa tcatttggat
      481 gttgataccc tcttcggcga gcgtgcggaa gattttggcg gctacaccga cgtgcgaacg
      541 catacccaaa ccgactgcgg agactttgca cacggtgtcg tcgccatcaa tagaagccgc
      601 gccgatactg tcttggcgtt ccgacaggat ttccaaagtc tgcttgtaat cgccgcgcgg
      661 tacggtaaag gaaaaatcgg ttgtgccttc gctgccgaca ttttggataa tcatatcgac
      721 ttcgatgttg gcatcggcga ccgcgcccaa aatctgatag gcgacaccgg gtttgtcggg
      781 tacgccgcgc acgttgatgc gggcttggtt tttgtcgaat gcgataccgg ttacggcagc
      841 tctttccatg ttgtcgtcct cttcaaaggt aattaaggtg ccattgccgc cgtcttgcag
      901 gctgctcagt acgcgcaggc gcactttgta ctttccggcg aattctactg aacggatttg
      961 caagactttc gagccgaggc ttgccaattc gatcatttct tcaaatgtaa ccgtatccat
     1021 gcggcgcgct tcgggtacga cgcgcggatc ggtggtgtac acgccgtcca cgtcggtata
     1081 gatttggcat tcgtccgctt tgagggctgc ggcaagcgca acggcggaag tgtcggaacc
     1141 gccgcgtccg agcgtggaaa tatcgccttc actgctgatg ccttggaagc cggcaacgat
     1201 gacgactttg ccggcggtaa ggtcggcacg cattttttcg tcatcaatgc tttcgatgcg
     1261 ggctttggtg tgggcagtat cggttttgag ggcgacctgc cagcctgtgt agcttttggc
     1321 atccacgccg atgtctttca atgccatcgc caaaaggccg atggttactt gttcgccggt
     1381 ggacaaaacc acgtccaatt cgcgcggatc gggatgctcc tgcatttcgt gcgccaacgc
     1441 gaccaggcgg ttggtttcac cgctcatggc ggatacgacg acgacgatgt cgtgtccttc
     1501 ggcgcgggct ttggcgacac gtttggccac gtttttaatg cgttcgggcg agcctacgga
     1561 tgtgccgccg tatttgtgta cgattaacgc catgtttcgt gctttcttgt gtgggattgg
     1621 tcgggcagca tggtttgctg gaaaagcggc tattattact attttttaca tgaagttcaa
     1681 gaacggactg cgctttcccg cctgccgttt gacagcggtc agcgaaaaac ctgttctttc
     1741 agattgttga caaaatgccg tctgaacggt tttcagacgg catccggacg acaatcaggc
     1801 ggcggacaac gcattttgct ggtgttgcag cagttcgcct atgccttttt gcgccagtgc
     1861 aaccagtttg cccaattcgt ccaaactgaa cggcgcgtct tccgccgtac cttggatttc
     1921 gatgattttt cccgatgcgg tcatgacgat attcacatca ctgtcgcaac cggagtcttc
     1981 gggataatcc aaatccaaaa gcggcacgcc gttcactacg cctactgaca cagcggcaac
     2041 ggcttcgcgg atggggtttt cactcaaaat gccgtctgaa accagtttgc cgacggcgat
     2101 ttgcagcgcg acaaacgcac cggtaatcga agccgtgcgc gtaccgccgt ctgcctgaat
     2161 cacgtcgcag tcaatcaaga tttggcgttc gccgagtttt tccatatcca cgaccgcgcg
     2221 cagcgaacgc ccgataaggc gttggatttc ttgtgtgcgc ccggactgtt tgcccgccga
     2281 agcttcgcgg cgcatccggg aagcagttga ggcaggcagc atcccgtatt ccgccgttac
     2341 ccagccttgg tttttaccgc gcagaaacgg cgggacgttt tcatctatgg aagcggtaca
     2401 aatcactttg gtattgccgc attcaataag gcacgaaccg tccgtatgcg gcaggaaatg
     2461 aggggtaatt ttgatatcgc gcaggctgtc cgcggcgcgt gaggtgcgga tgtaatcagg
     2521 catactgccc tcccgttaaa aacagataaa ttaaaaagcc tcaaacatga aaaactcaca
     2581 tttaaggcct tcaaactgaa aatttctacg cttcttcggc tttgctgcgg ataatcaaaa
     2641 gcggcaggtg gctttggcgc attaccgttt cggcaaaact gcccattaaa aggtgcatca
     2701 gcccggtacg tccgtgcgta cccaacacca gcaggtcggc accgttttca tcggcataat
     2761 caaccaaatc ctgcgccatt tcgcgcgcac ccttattggc aaccagcagg tgtttgacgg
     2821 tattttccac acccagttcc cgggcggtgc gctcggcggc atccaaaacc tcgttacctt
     2881 gtgcaatggc ggctgcctcg taactctcgt gttgcaaaaa ttcgggggcg agcgccatat
     2941 attcggcagg attggcaacg tgtaccaaag tcagacgcgc accgttgacc ccggcaagct
     3001 cggcggcatg tttcagggca ttgatggacg tttcactgcc gtcaacggca acaaccagat
     3061 gtttgtacat atcgtattct ccttttgcac cgcctcgcgg tgccctcttg tcggatgggc
     3121 gcagggacag tttgcgccgt ttcattatag acccgccgtc gggctttata caacagccga
     3181 acagcccgac cgctttccag tataatatgc cgcttccgtg cagtcaggca ttttttgccg
     3241 gctttcgttc actttttgat ttgacgcaat cttgcaggat tcgaccatgt ccgacaacgc
     3301 tttgacctct tcgcgacgct tcggcggcat cgccagactc tacggagact ctgccttggc
     3361 gcacttttca caggcacacg tctgcgtagt cggcgtgggc ggtgtcggct cgtgggcgat
     3421 cgaggctttg gcgcggacgg gcatcggacg tttgactttg attgatttgg acaacgttgc
     3481 cgaatcgaat gtcaaccgcc agctgcacgc cctgaccgga gacttcggca aagcaaaagt
     3541 taccgccttg cgcgaacgcg tcgcccaaat caacccagga tgccaagtgt ttgaaattga
     3601 agatttcgtt accgaagaca atttgccgga atacttcgga aaaggttttg atttcgtcat
     3661 cgacgcgatc gaccaagtgc gcgtcaaagc agcaatggcg gcttattttg tggaacgcaa
     3721 acaaccgttt gtcctcagcg gcggcgcggg cggacaaaaa aatccggcgt taatccaaac
     3781 cgccgatttg agccgcgtaa cccacgatcc gctgcttgcc aacctgcgct acaccttgcg
     3841 gaaacgctac ggcttcagcc gcgatactaa ggaaaaaatg cgcgtgccgt gcgtgttctc
     3901 aaccgaaaac atcgtaccgc ctcaatcggg cgtggcgtat tctgccgatg ccgcaccgca
     3961 gggcttgtcg tgcgccggct acggtgcaag catgctcgtt accgcttcgt tcgggctata
     4021 ttgtgcacaa gcagcggtgg aacacatcgc aggcaaaaaa taagcaatgc cgtctgaaac
     4081 aggattcaga cggcatttga acaaacgcgg caatcaagct gtcggttcag aaaaaccaac
     4141 atcggcacgc ctaaaaccgt attcacaacc tgctcctttt caaaacattt gcatttaaaa
     4201 gccgttataa tgccgtctga acatttgccc gaccacatta tacgtgaatg tcggcagatt
     4261 gttttctttt gtaaacttat attaaaatcc acttaccgat tcacgccatg ccgcccatcc
     4321 ctgccccatc cgcaccatcc gagcacactg tcgcatgggt attcggccaa cccgttaccg
     4381 atttgccgca ggatttgttt atcccacccg acgcgctgaa agtcgtattg ggcagttttc
     4441 aaggcccttt ggatctactg ctgtatctga tccgcaagca gaacatcgat gttctcgata
     4501 ttccgatggt gaagattacc gagcagtatc tgcactacat cgcccaaata gaaacctatc
     4561 agtttgattt ggcggcggaa tatcttttga tggcagcaat gctgattgaa atcaaatcgc
     4621 gcctgctgct gccgcgtacc gaagccgtcg aagacgaaga agccgacccg cgtgccgagt
     4681 tggtgcgccg cctgttggct tatgaacaga tgaagctggc ggcacaaggg ttggatgcgc
     4741 ttcctcgtgc gggccgggat ttcgcatggg cgtacctacc tctggaaatt gccgtcgaag
     4801 ccaagctgcc cgaagtctat attaccgact tgacgcaagc gtggctgggt attttgtctc
     4861 gggcaaaaca tacgcgcagc cacgaagtta tcaaagaaac catctccgtg cgcgcgcaaa
     4921 tgacggcaat cctgcgccgt ttgaacggac acgggatatg caggtttcac gacctgttca
     4981 atcccgaaca gggcgcggct tacgtggtcg tcaacttcat ctcactgttg gagcttgcca
     5041 aagaaggatt ggtcagaatc gtgcaggaag tcggtttcgg agaaatccga atcagcctca
     5101 atcatgaggg ggcgcattca gacggcattt ccggcacacg gggcgggcgc gatgtgttct
     5161 aatacgcccc aagccgccac caaaaatcgg gagacacgcc atatgaccgg catcatacat
     5221 tcgctgcttg acaccgacct ctacaaattc actatgctgc aagtggttct gcaccagttt
     5281 ccgcagacgc acagccttta cgaattccgc tgccgcaacg cctcgaccgt ctatccgctt
     5341 gccgacatca aggaagactt ggaagccgaa ctcgacgcgc tctgccggct gcgcttcacc
     5401 cacgacgaac tcggctatct gcgcagcctg cgtttcatca aaagcgactt tgtcgattat
     5461 ctcgaactct tccagctcca acgccgcttt gtcgaaatcg gcacagacga taaagaccgt
     5521 ctgaacatcc gtatcgaagg tccgatgata caggcgatgt tttttgaaat cttcatcctc
     5581 gccattgtca acgaacttta cttccgccgg ctggaaaccc ctgccgttat cgaagaaggt
     5641 gaacgccggc ttcaagccaa agccgcgcgc ctcaaagaaa tcgccgccgc acaaaacccc
     5701 gacgatccgc ccttcctgat ttccgacttc ggcacgcgcc gccgctacaa gctcgcgtgg
     5761 caggaacacg tcatccgcac cctgctcgaa gccgccccaa gcatcgtgcg cggcaccagc
     5821 aatgtctttc tcgccaaaaa actcggcatc acccccatcg gcaccatggc gcacgagttc
     5881 ctgcaggcat tccaggccct cgacgtacgc ctgcggaatt tccaaaaggc cgcgctcgaa
     5941 agctgggtgc acgaataccg gggcgatttg ggcgttgccc tgaccgacgt ggtcggtatg
     6001 gatgccttcc tgcgcgattt cgacctctat ttcgccaaac ttttcgacgg gctgcgccac
     6061 gacagcggcg acccttacgt ttggggcgac aaagcctacg cccactatca aaagctcaaa
     6121 atcgacagcc gcaccaaaat gctgaccttc tccgacgggc tggacatcga acgctcttgg
     6181 gcattacacc aatatttcaa agaccgcttc aaaaccggct tcggcatcgg caccaacctc
     6241 accaacgata tggggcatac gcccttgaat atcgtcttaa aactggtcga atgcaacggg
     6301 cagtccgtcg ccaagctgtc cgactctccg ggcaaaacca tgaccaacaa cagcaccttc
     6361 ctcgcctacc tgcgccaagt gttcgacgta cccgaacccg aaacgccgta aaccggcaga
     6421 aaaagcgcac aattcctgtt tctgccgcat aaaatctttt aaaataccgc ctgatttgaa
     6481 tttaaccgaa agaccgaact tcatgaacct acatcaaacc gtcgaacacg aagccgccgc
     6541 cgcctttgcc gccgcaggca tcgccgacag ccctattgtt ttgcagccga ccaaaaacgc
     6601 cgaacacggc gatttccaaa tcaacggcgt gatgggtgcg gcgaaaaaag ccaaacaaaa
     6661 cccgcgcgag ttggcgcaaa aggtcgccga cgcattggca ggcaacgccg tcatcgaaag
     6721 cgcggaagtc gccggtcccg gttttatcaa cctgcgcctg cgccccgaat ttctcgcgca
     6781 aaacattcag acggccttga acgacgcacg tttcggcgtg gcaaaaaccg acaaaccgca
     6841 aaccgtcgtt atcgactatt cttcgcccaa tctggcgaag gaaatgcacg tcggccacct
     6901 gcgttccagc atcatcggcg acagcatttc gcgcgtgtta gaatttatgg gcaataccgt
     6961 tatccgtcaa aaccacgtcg gcgactgggg tacgcagttt ggcatgttgg tcgcttattt
     7021 ggtcgagcag caaaaagaca atgccgcatt cgagctggcg gatttggagc agttttaccg
     7081 cgccgccaaa gtgcgctttg acgaagaccc cgcctttgcc gacaccgcgc gcgaatacgt
     7141 tgtgaagctg caaggcggcg atgaaaccgt tttggcattg tggaaacagt ttgtcgatat
     7201 ttcgctctcg cacgcccaag ccgtttacga cacgctgggc ttgaagctgc gccccgaaga
     7261 cgtggcgggc gaatcgaaat acaacgacga tttgcagccc gtagtcgatg atttggttca
     7321 aaaaggtctg gcagtcgaag acgacggcgc aaaagtcgtg ttcttggacg agtttaaaaa
     7381 caaagagggc gaacctgccg catttatcgt gcaaaaacaa ggcggcggct tcctttatgc
     7441 ttccaccgac ttggcgtgtt tacgctaccg tgtcggcacg cttcatgccg accgcctgct
     7501 gtacgtcgtc gaccaccgcc aagccctgca ctttgagcaa ctctttacca cttcacgcaa
     7561 agccggctat ctgccggaaa acgtcggcgc ggcatttgtc ggcttcggca cgatgatggg
     7621 caaagacggc aaaccgttca aaacacgcag cggcgacacc gtgaaactgg tcgatctgct
     7681 gaccgaagcc gtcgaacgcg ccaccgcttt ggtgaaagaa aaaaatcccg aattgggtgc
     7741 ggacgaagcc gctaaaatcg gcaaaaccgt cggcatcggc tcaatcaaat acgccgattt
     7801 gagcaaaaac cgcaccagcg attatgtgtt cgactgggac gctatgcttt cgtttgaagg
     7861 caacaccgcc ccctacctgc aatacgccta cacccgcgtg caaagcgtgt tccgcaaagc
     7921 aggcgaatgg gacgcgaccg cgccaaccgt tttgaccgaa cctttggaaa aacaactggc
     7981 cgctgagctg ctgaaatttg aaaacgtgct gcaaagcgtg gcggacacgg cgtatccgca
     8041 ctacctcgcc gcctacctct atcaaattgc gaccctgttc agccgcttct acgaagcctg
     8101 tccgatactc aaagccgaaa gcgcaagccg caacagccgc ctgcaactgg cgaagctgac
     8161 cggcgacacg ctgaaacaag gcttggattt gctgggcatc gatgtgttgg acgtaatgta
     8221 aaaccgcacc gcccgattgc ggacaacagc cccaccatcc ttatccgaat ctgaaaaaag
     8281 cggcgcgata caccgtatcc gccgcccctc ccaaaatgcg aaacaaacaa acgccaagca
     8341 agcaagcaag caagcaagca agcaagcaag caagcaaaaa attataaccc cccttactgc
     8401 cgacgcacgc actttccgcg cggcgcattc cccttttccc gcccctcaaa cccgcctttt
     8461 cttcaggcag ggtttcagcc cacctccttt ccctgttttc ctttccccga cacgcatgcg
     8521 cgccccctgc cgcaccgtgc cgcattttcg cgcccggacg gcatcgttcc gccatccggt
     8581 tctctgtttt aaatacccct gtttcagaaa gaaatgcaga tgtttcaaca cacaggacga
     8641 cacataaagc accgccctat gtgttgccct gatttggaag gggttacgcc tcccaaataa
     8701 agtctgatcc tgccgccccg aaggacagat gtccgagtgg cgaagtttca accgaaaagg
     8761 aaatacgatg aatattcaca ccctgctctc caaacaatgg acgctgccgc cattcctgcc
     8821 gaaacggctg ctgctgtccc tgctgatact gcttgccccc aatgcggtgt tttgggtttt
     8881 ggcactgctg accgccaccg cccgcccgat tgtcaatttg gactatcttc ccgccgcgct
     8941 gctgatcgcc ctgccttggc gtttcgtcaa aattgccggc gtattggcgt tttggctggc
     9001 ggttttgttt gacgggctga tgatggtgat ccaactcttc ccttttatgg atctcatcgg
     9061 cgccatcaac ctcgtcccct tcatcctgac cgcccccgcc ccttatcaga taatgaccgg
     9121 gctgttgctg ctgtatatgc tggcgatgcc gtttgtgttg cagaaagccg ccgccaaaac
     9181 cgacttccgg cacattgccg tctgcgccgc cgttgtggcg gcagccggct atttcaccgg
     9241 ccatttgagt tactacgacc ggggtcggat ggccaatatc ttcggcgcaa acaacttcta
     9301 ctacgccaaa agtcaggcga tgctctacac cgtcagccag aatgccgact ttattaccgc
     9361 cggcctggtc gatcccgtct tcctcccctt gggcaatcaa cagcgtgccg ccacgcatct
     9421 gaacgagccg aaatctcaaa aaatcctctt tatcgtcgcc gaatcttggg ggctgccggc
     9481 caatcccgaa cttcaaaacg ccacttttgc caaactgctg gcgcaaaaag accgtttttc
     9541 ggtttgggaa agcggcagtt ttccctttat cggcgcgacg gtcgaaggcg aaatgcgcga
     9601 actgtgcgcc tacggcggtt tgcgcgggtt cgcactgcgc cgcgcgcccg acgaaaaatt
     9661 tgcccgctgc ctccccaacc gtttgaaaca agaaggttac gccacctttg cgatgcacgg
     9721 cgcgggcagt tcgctttacg accgcttcag ctggtatccg agggcgggct ttcaagaaat
     9781 caaaaccgcc gaaaacctga tcggtaaaaa aacctgcgcc attttcggcg gcgtgtgcga
     9841 cagcgagctg ttcggcgaag tgtcggcatt tttcaaaaaa cacagcaaag gattgtttta
     9901 ctggatgacg ctgaccagcc acgccgacta tcccgaatcc gacattttca accacaggct
     9961 caaatgcacc gaatacggcc tgcccgccga aaccgacctc tgccgcaact tcagcctgca
    10021 cacccaattc ttcgaccaac tggcggattt gatccaacgc cccgaaatga aaggcacgga
    10081 agtcatcatc gtcggcgacc atccgccgcc cgtcggtaac ctcaatgaaa ccttccgcta
    10141 cctcaaacag gggcacgtcg cctggctgca cttcaaaatc aaataacaac aatgccgtct
    10201 gaacgcacca acagccttca gacggcattt tgcagacaga ccgacccttc aagcccactt
    10261 ttttcatcat ctccgataaa ttgctttgta tagtggatta acaaaaa
//
LOCUS       DAWFJW010000068        10161 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFJW010000068 DAWFJW010000000
VERSION     DAWFJW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.068"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(12..86)
                     /locus_tag="WGR19_001385"
     tRNA            complement(12..86)
                     /locus_tag="WGR19_001385"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Glu,seq:ttc)
     gene            complement(104..180)
                     /locus_tag="WGR19_001386"
     tRNA            complement(104..180)
                     /locus_tag="WGR19_001386"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Arg,seq:acg)
     gene            complement(290..1963)
                     /gene="recN"
                     /locus_tag="WGR19_001387"
     CDS             complement(290..1963)
                     /gene="recN"
                     /locus_tag="WGR19_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0798899.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGGWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2147..2749)
                     /locus_tag="WGR19_001388"
     CDS             complement(2147..2749)
                     /locus_tag="WGR19_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0798900.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2850..3791)
                     /gene="ylqF"
                     /locus_tag="WGR19_001389"
     CDS             complement(2850..3791)
                     /gene="ylqF"
                     /locus_tag="WGR19_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0798901.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3872..4726)
                     /gene="rapZ"
                     /locus_tag="WGR19_001390"
     CDS             complement(3872..4726)
                     /gene="rapZ"
                     /locus_tag="WGR19_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0798902.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4707..5669)
                     /locus_tag="WGR19_001391"
     CDS             complement(4707..5669)
                     /locus_tag="WGR19_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0798903.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRPD"
     gene            complement(5671..6120)
                     /gene="ptsN"
                     /locus_tag="WGR19_001392"
     CDS             complement(5671..6120)
                     /gene="ptsN"
                     /locus_tag="WGR19_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0798904.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEVRAILTEE"
     gene            complement(6305..7195)
                     /gene="ubiA"
                     /locus_tag="WGR19_001393"
     CDS             complement(6305..7195)
                     /gene="ubiA"
                     /locus_tag="WGR19_001393"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0798905.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGALLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7225..7701)
                     /locus_tag="WGR19_001394"
     CDS             complement(7225..7701)
                     /locus_tag="WGR19_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0798906.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7716..8363)
                     /gene="bioD"
                     /locus_tag="WGR19_001395"
     CDS             complement(7716..8363)
                     /gene="bioD"
                     /locus_tag="WGR19_001395"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0798907.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QQLAAQYEIVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLIFNHIHDSSDECVAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8360..9661)
                     /gene="bioA"
                     /locus_tag="WGR19_001396"
     CDS             complement(8360..9661)
                     /gene="bioA"
                     /locus_tag="WGR19_001396"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0798908.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLVDGMSSWWCAIHGYNHPVLNQAVETQIKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYRQVRGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPTSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            complement(9875..10114)
                     /locus_tag="WGR19_001397"
     CDS             complement(9875..10114)
                     /locus_tag="WGR19_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798909.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
BASE COUNT         2351 a         2786 c         2568 g         2456 t
ORIGIN      
        1 gcattttgaa ttggcacgcc cacggggaat cgaaccccgg ttaccgccgt gaaagggcga
       61 tgtcctaacc gctagacgat gggcgcatat tgtattttct tactggcgca cccggagcga
      121 ttcgaacgcc cgaccctctg gttcgtagcc agatactcta tccaactgag ctacgggtgc
      181 agcacaatca cttgttcaag tgattaagaa agcgtgataa tacggattta tctgagttct
      241 gtctattgtt ttagactttt atttttagtt attttttaac tgattgattt taaaataaac
      301 tatttttcga cgcaagttgc agcaattctg ccgcatgttg ccgcgtcgta tcggtaatga
      361 cttctccgcc caacatacgg gcaacctctt cgacccgttg gattttatcc aatatactga
      421 tttcgctgac ggtttgctct ccctcgctgt gcttgcgcac ccgccagtgg ttttcgccgc
      481 aggatgcgac ttgcggaaga tgggtaacgg caagcacctg atgttttctg cccaacgaac
      541 gtaatgcctt gccgaccatt tcagccactc cccctccaat accggtatcg acctcatcaa
      601 aaatcagggt gggaacttgg gtatattggc tggcaacaac ctgtaaggca aggctgatac
      661 gcgccaattc gccgccggag gcaactttat tcagcggacg gggcggattg cctttgttgg
      721 cggcaacttg aaattgaacc tgctccaaac cgtgtgccgt cggcgacgag ggcaacagga
      781 cgatgtcgaa acgtgcacct ttcatggcaa ggtgttgcat atgctcggtc gtttcgccgc
      841 tcaaacgctc tgccgcctga tggcgcatgg cagaaaggat gtgggcagct tcctgatatt
      901 cggcaaaatt gtgggcaaca ttatgctcga gcgcgtccaa atcggcggca gcttgcaggc
      961 tttgcaggcg ttcttcgatt tctgccaact tggcaggcaa ctcttcaggc tcgatccggt
     1021 atttccgcgc catccccatc agttcgccca tacgctgttc ctgtgccgcc aattcattgg
     1081 ggttgatgtc gctgcgacct gccacatcgc gcatattggc actgatttcg cccaattcgg
     1141 cctcgatgct tgccaacata ttcaggctct cggcaaagcg cggctcgatg ttttgcagat
     1201 tggccaatag tttttgacat tgatagatat ggcgttggat gccgttgtcg ccgtcaatct
     1261 tgcttccgac ttcttcggca gcctgcaaca gctcggcaga atgggcaagg ctgtcgtggc
     1321 tttggctgag ggcttcccat ccgccttgtt taatgtccaa ctgattcaat tcgttaaact
     1381 gccattccag acgctcttgt tcgagtgcaa tgttttctgc ctgttcctgc gcttcctgca
     1441 atgctttttt tgagtttgcc cagttttgat aaagctgcct gacggtttcc gcctgcgccc
     1501 tgctgcccgc aaatgcgtcc aacaattcgc gctgagcggc ttcctgatta agcgaatgat
     1561 gggcgttttg cccgtggatg tcgataagct ggctgccgac ggctttgagt tgcgccaagg
     1621 tagcggcctg attgttgata aagctgcggc ttttgccttt ggcatcgata atgcggcgga
     1681 tactgagttc ttcttctccg tcgtttaaaa gcccctgttc atacaattct gcttttaaaa
     1741 caggtaaatg ggaaatatca aacaacgccg acaactgcgc ttcttttgcg ccgctgcgga
     1801 cttggctgta atcggcttta tcgcccaaca gcagaccaat cgcatccaaa gtaatggact
     1861 tgcccgcgcc agtttctccg gtcaatacgg taaagccgct ttgaaaatcc agattcagat
     1921 tttcgacaat gacaaaatca cgcaaagaaa gtgttagaag catcgtgagg attccttagc
     1981 aattctaccg acaaaagcaa tatcaacttt agatatacga attataacta agatttttta
     2041 aattaaaaat aagtttttcc ttaattttgc ttagtgcttg tttttatctg cttatttgtt
     2101 gaaatgccgt ctgaagcaat gtgcgtttca gacggcattt ggaatttcag ttgggcaggg
     2161 tatcggacgg tacggcttcc ggctcttctt catccgtagg cggcatttcc attaaatcgg
     2221 gtaaaaccag ttcgcccagt tcggtcagcg gcggaagttc ttccagaccg tccaagcgca
     2281 aatcgctgag aaaagttgcc gttgtcgccc acaatgcggg ttttcccaat gtgtcccgat
     2341 gtccgatgac ttcaatccac ccccgatcct gcaaggtctg catcacgttc tgcgacaccg
     2401 ccacgccgcg tatgccctcg atgtcgccgc gcgttacggg ctgctggtag gcgataatcg
     2461 ccagtgtttc catcacggcg cgggagtaac gcggcgcacg ctgttcttgc aggctgccca
     2521 gccgctcgaa tgccgtctga acaatctgaa aacgccagcc ctcttgcgta tgcaccagtt
     2581 gcaacgccct atcctgccaa cgcgttttca actgcgccaa cacatcaatc agtttgtcct
     2641 gcgacagtgg cggcacacac aattcgcgca tagatttttc ggtcagcggt tcggtttggg
     2701 tcagcagtgc ggcttcaatt agtgcgtcgg gagaaatttt gtcggtcata cgggtatccg
     2761 tgctgaaacg gcatgggttt ggatatgccg tctgaaatcg gttggagtag agagaagctg
     2821 cctgaaaaat atttttcaga cggcattctt tatgcttccg aaggcttctg ccctttacgc
     2881 tctgcttttc tggcttcgcg tatggctttg agttcggctt ctttctgacg ggcttttttg
     2941 agccaagttt cccattggtt cggcgtttcg agggtgattc tgccgatttt gccttcacgg
     3001 aagtcggtga ggatgttttc ggcggctttt tggtagttga tccgtccgcc gctgaggact
     3061 gcgccgcgtt ttttggctat ccattcgagc caaacgtttt cgtcccagtg gctgctgggg
     3121 tctttgtcgg cttggtagcg ttcttgcaac atagggaggt agtggcggcg gaggtagtct
     3181 aaaagttcga gggcgacttc ttcttcgtcc aacgcgttgc gtccgactgc gccgccggcg
     3241 gcaaggttgt agccgccttc ttcgacgatg attttcggcc acagcattcc gggggtgtcg
     3301 tagagccaga agtcgtcggc aaggaaaagg cgttgttcgg ctttggtgat gccgggttcg
     3361 ttgccggttt tggcggattt tttgcctatc atgccgttga tgagggtgga tttgccgacg
     3421 ttgggaatgc cgcaaatcaa gacgcgcaag ggtttgtcta tgccttgccg gtggggaatc
     3481 atcgcgcggc aggcttgggt gattttgccg tgtgcgccgg tttcggagga atcgagggca
     3541 atggcgcgcg tgtcggggcg gctgttgtag tggtcaagcc agacctttgt gcgttcgggg
     3601 tcggcaagat cttgtttgtt taggatttta agtttgggtt tgcctttgga aagctgggca
     3661 agcagggggt tttcgctgga ggcgggcata cgcgcgtcca gcatttcaat caccatatca
     3721 acgctttttg cacgctcggc gatggctttt ttcgccttgt tcatatggcc gggaaaccat
     3781 tggattgcca tgtctgttct ttcttttcaa tatttgaaat gccgtctgaa acggaggacg
     3841 gggtttcaga cggcataatg gtttgacgga attagcggtc tgacaggttt tgcgcctgtc
     3901 tgtgccgtat cagcaattca taacgccctt tcaaccttcg ggcgagtttt tcgacaacat
     3961 agacggaacg gtgctgcccg ccggtacagc cgatggcgac ggtaacgtag ctcctgcttt
     4021 catcctccaa acgcggcagc caatgcgtaa caaacctttc gatgtcgtca accatttcct
     4081 gcacaagcgg ctgtccgtcc aaataatccc aaacgggctt gtccataccg gtgtaaggcc
     4141 tcaactcggg atcgtaatac gggttgggca gactgcgcat atcgaacata aaatccgcgt
     4201 tgtttggcac accgtatttg aacccgaagg actccaaaat caccagcagc ccggtacgtt
     4261 cgaccttcag ccactgccgg actgcatggc ggagctgttg ggcattcatc ttggaagtgt
     4321 cgatacaata ggcgatttct ttaagcggga acagccattc ccgttctttc tttaagcttt
     4381 ccaacaaggt catatcctga ttactcagag ggtggcctcg cctggtttcg gaaaaccggc
     4441 ggaccaacac gctttcttcc gcctcgacaa acaaaacttc aaccctgtgc cccagtccgc
     4501 gcagagaggc aatctgttcc cgcgcctgtc cgatgtctat gccggaacgc acatcgacgc
     4561 tgaccgccaa ttcggtttcg tccgcacgct cgatatgata cgacaccagc gcgggcaaca
     4621 tttccaaagg caaattgtcc acgcagaaat aacccgaatc ttccatttgg cgcagtgcga
     4681 cggatttgcc cgaaccggac aacccgctaa tcaggacgat tttcattgtg ttgctcgttt
     4741 tctttaagct gcgtctgatg acgttccaaa aattcgcgcg tactatcctt accgcgcaac
     4801 tgaagaatat aattgcgtac cgccgcctca accaaaacgg cgaggttgcg tccgacggcg
     4861 acgggcagcg taaccgaacg gacgttgacg ttgaggatgg attcggtttc ggtgcggatg
     4921 ctcaaccggt caagctgctt catatactcg tcgtccgcct cgaccaaatt gataatgagt
     4981 tgcaggattt ttttggggcg gatggaagtt tcgccgaaaa tatggcggat attgagtatc
     5041 cccaagccgc gcacttccaa aaaatcgcgc agcataggcg aacaacgccc ttccaatgtt
     5101 tccggaccga tgcggaacag ctccaccgca tcgtcggcaa tcaggctgtg tccgcgcgaa
     5161 atcagttcca atgccaattc gctcttgccc aaacccgaat gcccggtaat cagcacgccg
     5221 atttcaaaca catcgagaaa tacgccgtgt ttgacggacg atgccgccag ggtgcgttgc
     5281 aggtaaatcc gcaacacgtc catcagatag gggctttcga gtttggaagt cagcagtgga
     5341 atatcgtttt tgtggcagta gtcgcgcagt cccggggaaa ccggcaagcc gtttgccaca
     5401 ataaccaaag acatagaaat atcgaacagg tcgccaaact gataacccgt ttcccccgat
     5461 tcgaggcggt tcagatattc cgactctgcc aaaccgacca cttggatttg gttgggatga
     5521 atgaaattca ggtgtccgac tagggcgagg acgggcttgt ccgcctctac gccgatacgg
     5581 ttgtccgcac ccgaattgcc ggcggcccaa gcgagttgca gtttgtattg gttgtcatca
     5641 aacaggcggc ggacggagat actgggcata ttattcttca gtcaggatgg cacggacttc
     5701 ttccgcagag gaaaccgtca tcagcgattc tctgatgctt ttttgggaaa acttgccggc
     5761 cagtttggat aagacttcca aatgctcgcc ggttgcgttt tccggaacca gcaagataaa
     5821 aatcagggaa accggcttgc cgtccggtgc gtcaaatccg acgggttcgc gcgtgcggat
     5881 gaacgcgccc gtcgcctgct tcacgccggc atgacggccg tgcgggatgg caacgccctg
     5941 ccccaaaccg gtcgaaccga gtttttcacg ggcaaaaaga cattcgaaaa catcagcatg
     6001 ggacaatgag gattcgcgtt ccaaaagcag gcctgcttcc tcaaacagcc tttttttact
     6061 gcctacctcc atatccaaaa caatatggga caaaggcaaa atttcgccga taaggctcat
     6121 aagcttctct tttcagacat cgcaaaacag aaagattgta ccgactgccg gggcaaatct
     6181 caatcccgca tacggtacgg gctgacataa cacagcgttt taaaaacata ttttaacgct
     6241 tttcggcaca gatagaaatg ccgtccaaag cagtttacgg ctcttcagac ggcattgccc
     6301 tgccttattt cgcgaaaaac gtatgggcaa aaatggcggt aaaccacacc caaccaattc
     6361 tgttgtttgc caaaaacgtt tcaaaacaga tttgccggac gcggcttttg atggcggcat
     6421 attggcggta ttgcagcagc aggacgatgg ggattgccgt ccaatatgcc catgccgcac
     6481 cgataaccgc acccaatact gccatcagca gggtaaagcc tccgtgacac agcataacgg
     6541 cggcgatgtc gtaacgcccg aacgtgacgg cggaggtttt gatgccgatt ttcaaatcgt
     6601 cttctttgtc cgccattgca taaaccgtgt catacgccag agtccataac acattggcgg
     6661 caaagagtat ccacgcttga ggcggcacgt ttccggcaac ggcggcaaac gccatcggga
     6721 taccgaagga aaaggcaagc ccgagataga gttggggaat cggaaaaaaa cgtttggtaa
     6781 acgggtaagt cagcgcaaga aacagcgcgg gcaggctcat cagccaagtc agatgattca
     6841 gcggaatcag gcacaatgcg gcaagcaggc acaaaaatgc cgtcagcagc agcgcttctt
     6901 ttttcttgac cctgccctgt gcgaacggac ggttttttgt acgctcgaca gcaccgtcaa
     6961 aatcgcggtc ggcaaagtcg ttgatgacgc agccggcact gcgcattaaa aacgtgccga
     7021 ttgtaaacgc cgccaatacc gccaaatcgg gaatgccgtc tgaagccagc cacaatgccc
     7081 agtaggtcgg ccacagcaaa agcagcgccc caatgggctt gtccgcccgc atcaggcgca
     7141 ggtacacatc caaacggtcg gacagtcgta aaaataaagg ggatttagga ttcatattgc
     7201 cgcacagctt gaaaaaacgg tattttatcc gataaagcgt ttcagttcgg gcagaaaata
     7261 ctcggtcagc agcatttcct cgccgtgacg ggaaaaccga gaacgccgcg cggcaaagta
     7321 ccgtccgcat ccttcgccgg aaacggcaaa ctcaaacgcc gaacgcgccc cttccaaatc
     7381 ggcttgaaac agacgctcgc ccaaagggcg cgtgccgcag tccaaaatgt tttgccaaaa
     7441 cgccgaacct atacggcact cgctccttgc ctcaacaaca gggataccgt ccagcttcaa
     7501 caaaacttcg cgcaccagcc tccctccgca ttccgtctcc aattcgccca gtttcagcag
     7561 ttccaccgaa aatgtatgcg gcaaggcgcg caatgcggcg gtcagcgacc gggctttcaa
     7621 taaccggctc atcggcaggt cgatgccgtc tgaaacggcg gcgggcaagt cgggcagcca
     7681 tttcccaaat aggtgttcca tatttttccc aatctttata ccgcgtctgt ttttgccaac
     7741 tccatccatt ccgcttcgga aaaatcggct ttcagacggc atttcaagta gctcaggctg
     7801 tcttgggcga cacattcgtc ggaactgtcg tgaatatggt tgaaaatcag gctgtgcagg
     7861 cggatgccgt attgtttgag cgcggcgaaa ctgagtaaag tgtggttgat actgccaagc
     7921 cgtccgctgg taacgaggat gacgggatag ccttgctgac ggatataatc aatggttaac
     7981 aggttttccg tcagcggaac catcaatccg cccgcgcctt cgaccaagac gatttcgtat
     8041 tgcgccgcca gttgctgcgt ggcggtgcgg attttgtcca agtccaaagc cctgccgtca
     8101 agacgggcag cgagatgagg cgaagctgga taactgaaga tttcaggcat ggtcagccct
     8161 tgtttgtccg cttcctgcat cgggatgccc atgattttgc ggtgaacggc gatgtcttcg
     8221 gcgatgtctt ggcaaccggt ttgcacgggc ttttgcgtga tcacgctttt gccctgctgc
     8281 aacaattgtt ttgccaacat accggtggcg acagttttgc cgatgtccgt gtctatgccg
     8341 ctgacgaagt aaacgccttt catttgctgt gttccttcaa gatttgcacg gttttgtcgg
     8401 caagtttggt caaaacgccg tctgaaataa tatacggcgg catcagatat accagcctgc
     8461 cgaacgggcg cacccaaatg ccctgcgcca cgcaatctgc ttgaaaacgc gccatatcca
     8521 cgcctttatc cagctcgatc acgccgatgg cacccaaaac gcgtacgtct ttcacgccgc
     8581 gaatgtccca cgcggctttc agacggcctt ttaagatgct ttcaatgcgg cggatatttg
     8641 cctgccagtc ttgagacaaa agcagtttga ccgaagcgca ggcaacggca cacgccagcg
     8701 ggtttgccat aaacgtcggg ccgtgcataa acacgcccgc ttcgccgcgc gaaatcgttt
     8761 cggtaacttt ttgcgaagtg attgctgccg ccagcgtcat atagccgccg ctcaaaccct
     8821 tgccgatgca cataatatcc ggcacgacct ccgcgtgttc gcaggcaaac atcttgcccg
     8881 tgcgcccgaa tccggtggcg atttcatcaa aaatcaaaac gatatcaaac tcgtcgcaca
     8941 aatcgcgcaa tccgcgaaga tactgcggat gataaaaata catgccgccc gcgccctgca
     9001 cgaccggctc taaaataaag gcggcaatat ccgcatgatg cacttcaaac aaggcgcgga
     9061 caggctgcaa atccgcctcg tcccattcat cgtcgaaacg gcttgtcggg ttatcgacaa
     9121 aataacgctg cggcaacgcg ctgccgaaaa tatggtgcat ccccgtttcc ggatcgcaga
     9181 cggacatcgc gttccaagta tcgccgtgat acccgcgccg caccgtcgca atattctgct
     9241 tcgccgtcaa cccccgcacc tgccggtatt gcaccgccat tttcaacgca acttccaccg
     9301 aaaccgaacc tgaatccgca taaaaaatac ggtccaaccc ctgcggcaaa atcccaacca
     9361 acaatttacc cagctccacc gcaggctcat gcgtcaaacc gccgaacatc acatgcgaca
     9421 tttgtttaat ctgcgtctca accgcctgat tcaaaacagg atgattgtaa ccgtgtatcg
     9481 cacaccacca ggacgacatc ccgtcaacca gccgcgtgcc gtccgccaat tcgataaaca
     9541 ccccttctgc acgtttgaca ggataaacgg gaagcggatc ggtcatggaa gtatagggat
     9601 ggagcagatg ggtacggtcg aaatcaagta atgatgatgt gtgttgatgt tcagacggca
     9661 taagtttctc tcttttctta ttgctgcatt caaacgcaaa cgcgtattct actccgacag
     9721 accgtttccc acacctctcc atccgtttcg ggcgcaaaac cgcgaaacaa atcgttccgc
     9781 aacataagcg cacaccgttt cgcattcctc aagcccgatt ggaatcagac ggcccaacgc
     9841 ccaataccgt ttcacgcagc ctccgcccaa cgcttcaatc agtcatagac aaaacggcaa
     9901 tccatacgat acagatcata gcaacagcca tcgcaacagc gttggcaaaa tcaggggact
     9961 ccgacatagg cgcatagcat ctgccgatgc acggctcctc attcggctct atgaatacca
    10021 tacccatcac aaaatccacc gccaaaacca ggcacggctt cttatactta tgatagattt
    10081 ccaccatcct gccccatata tcccaaacat tcataccgca tatcccgcag gcaacaaatt
    10141 ccgattgaag gttacagccc t
//
LOCUS       DAWFJW010000069         9798 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFJW010000069 DAWFJW010000000
VERSION     DAWFJW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.069"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(13..1308)
                     /locus_tag="WGR19_001398"
     CDS             complement(13..1308)
                     /locus_tag="WGR19_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0798910.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHEAVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLERAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRSQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPERMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WGR19_001399"
     CDS             complement(1434..2321)
                     /locus_tag="WGR19_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0798911.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2321..3286)
                     /locus_tag="WGR19_001400"
     CDS             complement(2321..3286)
                     /locus_tag="WGR19_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0798912.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGR19_001401"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGR19_001401"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0798913.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4621..4728)
                     /locus_tag="WGR19_001402"
                     /pseudo
     CDS             complement(4621..4728)
                     /locus_tag="WGR19_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WGR19_001403"
     CDS             complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WGR19_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0798914.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WGR19_001404"
     CDS             complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WGR19_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0798915.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WGR19_001405"
     CDS             complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WGR19_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0798916.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WGR19_001406"
     CDS             complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WGR19_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0798917.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8697..>9798
                     /locus_tag="WGR19_001407"
     CDS             8697..>9798
                     /locus_tag="WGR19_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0798918.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAVAEYRLNRIAVIDFDVHYGDGTAKIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRAFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2509 a         2600 c         2434 g         2255 t
ORIGIN      
        1 tgcctgcgac gctcaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgcgt ataaaaaaca ttcggggcta aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcggcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgctc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctgacag caatcgcctt
      541 gcgttccaaa ggccggaata tcgggttcaa acctgcataa gtattgacag catagaccac
      601 atttttgcac tcgacgctgc cttcgggcgt gtaaaccagc caaccgtttt gatgcggttc
      661 gatgcacgtc atcggggatt gctcgaaaat ctgcgcaccg gcttcggcag cggcacgagc
      721 gatgcccaaa gtgtaattga gcggatgcag gtgtccggat aaggggtcga attgtgcccc
      781 ttggtacata tcgctgtcaa gctgctgttt caactcggct ttatcccaaa gttgataatg
      841 actcgcaccg taatgccgtt gggcgtgttc atgccactgc tgcaactctt cccaatgctg
      901 cgaacggacg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg taaagactgc tgccaaaacc attgtgcccg
     1021 ctccaaacca acctgttttt caatttcctc cataccgcag gcgtagtcgc tgataacctg
     1081 accgccactc cgtcccgatg cgccgaaacc gatacgcgcg gcttccaaaa cgacggcttc
     1141 atgtccctgc tccgccaagg gcagtgcagt gcacaaacca ctcaaaccac caccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actaaaataa taagaaggca gatattcttg aaaatcagga cgaatcatga tgtttacttt
     1321 atcaggtata ttttcagacg gcatgatgcg gtttgccgta ccatgccgtc tgaacgggaa
     1381 attactttaa gaaaacaggc tgacctgata atgcggtcag cctgccctgt cggtcaatgc
     1441 gctttctcgg cagccagttt ttcctggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gtcgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatc
     1561 aggatggttg ccaaaacgtt catctgagga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtagaaga accggggccg gacaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcacccaaat ccattgcggc ttcttcgagc
     1801 gactggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtacgc catacacagc
     1861 gtcgtatgtc cgaggaagat ggtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcagcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa taccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gcgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accgcacgct
     3241 gccccggacg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa caacggagtc ggttggtttt ccggccagct gatatagacg gtttcgtccc
     3361 aagtcggcgg tgtaatgttg cgcacatacc agtaaggggc ggggacttgg cttttgacga
     3421 cgcgcccgtt gccgagcttg atatggtaaa tggcgaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac cgccggacaa ttggtgcggt ttgcgtttag
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccgaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcgatc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata atttttccct gattgggact ttccataccc gccagcatac
     4261 gcagcagtgt agattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaaacctt
     4381 ggattttgag ataaggtttg gctgaagatg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaga cgagtaccgg caaaacggat gttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agttaaacgt ttgatgccgt ctgaaactct tgtaaagcgc acgggcagca
     4561 tgaaatggaa caagattcca aagaacttta tattatatta gtttatgcgg ttttcgggca
     4621 atatagtgga ttaaatttaa accagtacag cgttgccttg ccttgccgta ctatttgtac
     4681 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactataaa tatggatttg
     4741 agcttgtcgg aaagcaacag aaaagaaaac accgcccatt tttctgggcg gtgtcggaaa
     4801 gcgtaattat ttacgcagac ccaagcgggt aatcaacgcg cgatacgtat cgggctgggt
     4861 acggcgcaag taggccagca ggcggcggcg ttggctgacc attttcaaca ggccgcgacg
     4921 gctgtggtgg tctttggggt tggctttgaa gtggggggtc aggtcgttga tgcggaaagt
     4981 caacagagcg acttgtactt cggaagagcc ggtgtcgcct tctttgcgtt ggaaatcttt
     5041 aacgatttgt gctttttgtt ctacggtcag tgccataatg aaaactccaa aaatataaga
     5101 atccccatag ggattcagac aagtttgcca agcctgaaga caacggcaaa ctccctatgc
     5161 ccaagatagg acaacgtggc attatgacac aattcccgca tttctgcaca atattttaag
     5221 accgtggaag taaccgttaa aaatgccgtc tgaagcattg tctgcttgag acggcaatgt
     5281 tcagacggca tatgcgcttc aaaccacgat ttcttctttc ttcttcggtt ctttaccgat
     5341 attgtcgcgg ctcaaaccga acattagcag aagcgggctg gcaaccaata cggaagaata
     5401 aatgccgaac acgatgccaa tggtcaacgc catagaaaag ccgtgcaagg ccgcaccgcc
     5461 gaacaccagc atggatacga ccatcgcctc ggtcgaaccg tgggtaatga tggtgcggct
     5521 catcgttgcg gtaatcgcgt tgtcgatgac ttccggcacg gcatgtccgc gcatcgccgg
     5581 cttgcggaag ttttcacgga tacggtcgaa gacgacgacg gattcgttca cagaatagcc
     5641 caatacggca aggatacccg ccaagacggt cagcgaaaat tcccattgga agaaggcaaa
     5701 gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata cggcaaaacg
     5761 ccattcaaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac ctaaagccat
     5821 caatccatta cttaccaatt cctcaccgac ttgcgggccg ataaattcga cttggcgcaa
     5881 ggtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca actgtgcgga
     5941 agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac ccaatgcctg
     6001 aacctgtaca tcacctattt tcagcgtatc gaggcgttcg cgcatcttat tgacatccgc
     6061 accctgctga tattggactt ccattaccgt accgccggta aattcgacag agaaattcag
     6121 acctctggta accaaaaaga atacggcggc aataaaggta accaacgaaa taaaggtcgt
     6181 cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt ccatagctta
     6241 ctccttgcct cctgccattt cggctttcgg cttccacacc gaaccaatgg aaatattctg
     6301 caatttgcgt ctgcgtccgt accacagatt gaccaacgca cggaatacga cgacggacga
     6361 atacatcgaa gtcagaatac ccaaacagtg tacgaccgca aaaccgcgta ccgggccgga
     6421 accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttggaat cgacaatggt
     6481 cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc cggcacgcaa
     6541 ttcttcgcgg atacgttcgt taatcaagac gttggagtcg attgccatac ccaaagtcaa
     6601 cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca aaataccgat
     6661 taggaacagt atgttggcac tcaatgcaat ggtagaaaag aaacccatca gacgatagta
     6721 aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga agcctttttc
     6781 gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct gcatcggcgc
     6841 ggcaagagaa ccggcacgca acagcaaaga cgtatcattg gcttcggctg tcgtcatgct
     6901 tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggcg cggttacaac
     6961 ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat ttgcggcagt
     7021 cagttcgcca aaaatgctgc cacccgcgct gtccaagctc agactgacgg caggtgcgcc
     7081 catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg cccgtcagct cgacctgttt
     7141 gctgattagc agagtttcgg gacgatctcc gccgcttgaa agcagctcat aaccgctcgg
     7201 cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca tacgcaattc
     7261 caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcctgaa cgcccggaag
     7321 ctgcacgacg atacggtcgg caccggactg ctggatgacg ggttcggcca cgcccaactc
     7381 gttcacacgg ttgtgcaggg tagtgatgtt ctgtttgacc gcatcggaac gcactttatt
     7441 gaccgcctct tccgaaagcg tcaagacgat attgctgccg tctgaattca gcgttgcttc
     7501 aggaaacagc ttgcgcaact gcggcagtgc cttttgcaca tcacctgcat cctgcaaagg
     7561 gacggtcagg ctgtttccag cctgacgcac cgtgccgctg cggatttttt cgcggcgcag
     7621 ttcgcggcgg atgtcgcccg aataacgttc aaacgttttc tgcatcgccg ctttcatatc
     7681 gacctgcatg gtgaaatgca cgccgccgcg caagtccaaa cccaaaaaca tcggattggc
     7741 tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg taatataccc
     7801 ttcgcccaaa gtgttttcga tgacgtcgcg cgctttaagc tgcgtttctg tgtctttgaa
     7861 acgcactttc agtgaattgt ccacaacaaa catcccgtcg gtctgaatac ctgcgttttt
     7921 cagcgcggca tccactttga attgagtctg ttcgttgatg atgatggctt gtcggttggt
     7981 cgatacctgc acggcgggtg tttcgccgaa taggttgggc agcgaataca ctgcggcaac
     8041 cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca tcattgttcc
     8101 ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc agaagaggca
     8161 aaaacccttc ccagccaggc aagaccggaa ggcggcatct tgaatatgcc gccctgcgtg
     8221 tcggaacatg gtcaagcctt cggttggaat tcaaaacaaa gtgccgcatt cgggctttcc
     8281 agatgcggct tgtcggcaca aatcaatcga cttttgcggc aatcgcattg cgttctactt
     8341 cgacctcgat ttttgtaccc tgtccgatat ccacggtaaa aaactgttcg ccgactctgg
     8401 ttaccttacc cttgaaacct gccgccaaga ccactttgtc gccgactttc aaggcggcaa
     8461 gcattgcctg atgcgctttg aatttctttt gctgcggacg catgatcagg aagtagaaca
     8521 ccaccataat caacactaaa ggagcaaatt gtgcaacagc ttgattcata atttatccgt
     8581 tctttctaat atggttgaaa atcgagaggg gtatataata acataagacc gtaaacaata
     8641 tatcgggttt gccgtccgta ccgaccgtat accgcagcct gcccgtccac aaacccatgt
     8701 ccttgacccg cctaatcttg aaattctatg cactgttgcg cctttttttg ggcaaaaacg
     8761 cccgcaccgc atggatttcg catcccgcct gtgccgggca cgaacccggc gcaaaccatc
     8821 ccgattcgcc cgaccgcatc ctctgcatcg agcaggcatt gcgccgcgcc ggtatttggc
     8881 agcacctcca aaccatagag gcggaagaaa tcagcgatac gcgcctcgca cttgtccact
     8941 cgagcaaata tctgaaccgt ttggaatctt gcctgcccca aaaaggcaag atttcccgcc
     9001 tggataacga cactgcaatc agcacaggct cgctgtctgc cgcacgcttt gccgccggtt
     9061 cggcagttca ggcagtcgac atggtcatga accgtaaagc atggcatgcc ttttgcgccg
     9121 cccgcccgcc cggacaccat gcgggcagcg gcaaagccgg cggattctgc ctgctgaaca
     9181 acgttgccgc cggcgtcatg catgccgttg ccgaataccg gctgaaccgc atcgccgtca
     9241 tcgatttcga tgtacactac ggcgacggta cggcaaaaat attcaaagac gatccgcgca
     9301 tcctgttttt caacctgttt gaaaccgacc ttttcccctt ccccgaaaac aacgatatgc
     9361 ccgacggcgg caatatggtg cacctgccct tgccgccggg aacgggcagc cgcgcattcc
     9421 gcgaagccgt ccgcagacag tggctgcccc ggcttgccgc attcaaaccc gaactggtgc
     9481 tgctgtcggc aggattcgac gcacaccgtc tggacgaatc gggcaggctt aacctgcacg
     9541 aggcggattt tgcctggctg acacacaaaa tcattcagac ggcatcgggc tgccctggca
     9601 aaatcgtatc cgtactcgaa ggcggctaca cccttgaacc tctggcacaa tctgctgccg
     9661 agcatatccg cgtgctggca gggctgggca aatccgatgc cgcaaccgcc tatcagaaaa
     9721 cactggaccc aacaaaaaaa cggtttgcca aaccgaaaac cggacaggtg cgccaaccta
     9781 cccaatccga ccgttacg
//
LOCUS       DAWFJW010000070         9744 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFJW010000070 DAWFJW010000000
VERSION     DAWFJW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.070"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(79..276)
                     /locus_tag="WGR19_001408"
     CDS             complement(79..276)
                     /locus_tag="WGR19_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798919.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPEMGDIGHTKLIVNAGCFDNFEDAA
                     NAGLEEIGNENEFIVFTFFEV"
     gene            complement(281..571)
                     /locus_tag="WGR19_001409"
     CDS             complement(281..571)
                     /locus_tag="WGR19_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798920.1"
                     /translation="MNFNMRGQIIGVKKFSGNIEGKEFDYCRIIVATPLDVTQGNALG
                     MSAVEYNYGASANFEHFKNQKFPIEADLDVEIVTTGKTQKVNVLGFSPVKKV"
     gene            complement(617..1939)
                     /locus_tag="WGR19_001410"
     CDS             complement(617..1939)
                     /locus_tag="WGR19_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0798921.1"
                     /translation="MEDKQGMTKAVAGVMTDALADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQITFSIHEDTFFHVYGLSFD
                     LFDDDDFIRAASAKMEEIFGFGIIEKAKHSGGRFYQGCWLMGTENAQYGRVHFGGQNN
                     TMLFELTAVGCNAANIGWESRLFDFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGLFTCHHVKPKGECLGFDWEEEDEAKMTRGKTYGIGSRESSKYVRIYEKGKQLGDKT
                     SIWTRFEIEFKAKDIVIPFEVLQTPGEYFGGAYPICERFTGSANRIEAVKSKIMIDFD
                     TYIERLKKQIGRGINALKAVFPDKSKQELFEVLEPKHDFLPKKLSFENYDCSEAKATP
                     MHEIPSVLKFDEYGMWMDRYIRRQKRGEEQRYLEKMYDKYANLPISWA"
     gene            complement(2360..2566)
                     /locus_tag="WGR19_001411"
     CDS             complement(2360..2566)
                     /locus_tag="WGR19_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0798922.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(2661..3830)
                     /gene="metZ"
                     /locus_tag="WGR19_001412"
     CDS             complement(2661..3830)
                     /gene="metZ"
                     /locus_tag="WGR19_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0798923.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            complement(3889..4079)
                     /locus_tag="WGR19_001413"
                     /pseudo
     CDS             complement(3889..4079)
                     /locus_tag="WGR19_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4130..4891)
                     /locus_tag="WGR19_001414"
     CDS             complement(4130..4891)
                     /locus_tag="WGR19_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798924.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            4991..5242
                     /locus_tag="WGR19_001415"
     CDS             4991..5242
                     /locus_tag="WGR19_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798925.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            5294..6100
                     /locus_tag="WGR19_001416"
     CDS             5294..6100
                     /locus_tag="WGR19_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0798926.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            6407..7930
                     /locus_tag="WGR19_001417"
     CDS             6407..7930
                     /locus_tag="WGR19_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0798927.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            8028..9440
                     /gene="trkA"
                     /locus_tag="WGR19_001418"
     CDS             8028..9440
                     /gene="trkA"
                     /locus_tag="WGR19_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0798928.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         2439 a         2721 c         2292 g         2292 t
ORIGIN      
        1 gtattcatga tgttcatgat ttttttccta tcggcggtaa ttaaaaaatt tattcgggcg
       61 accgccatcc gcccaaattc agacttcaaa aaacgtgaaa acgataaatt cgttttcgtt
      121 cccgatttct tccaatcccg cgtttgctgc gtcttcgaaa ttgtcaaaac aaccggcatt
      181 cacaatcagt ttcgtgtgtc ctatatcccc catttcaggg gataaataca gaaaatcccc
      241 tgttgatacg gactgaacaa catagacttt ctgcatttat ttaaactttc ttcacgggag
      301 aaaatcccaa cacgttcact ttctgggttt tgccggttgt tacgatttct acatccaaat
      361 cggcctcaat cgggaatttc tgatttttga aatgttcgaa attggcggac gcgccatagt
      421 tgtactcaac ggcagacatg cctaaggcat taccctgagt tacatctaaa ggggtagcca
      481 cgataattcg gcaataatcg aattccttgc cttcgatgtt tccggagaat tttttaacgc
      541 caatgatttg gccgcgcata ttaaaattca tgatttttcc taaaaaataa aatttaacga
      601 cattagggct gccgttttaa gcccatgaaa ttggtaaatt tgcgtatttg tcatacatct
      661 tctctaaata acgctgctct tcccctcttt tttgacgccg aatatatcga tccatccaca
      721 tgccgtattc atcaaatttt agaacggacg gaatttcatg cattggcgtt gcctttgcct
      781 cgctgcaatc gtaattctcg aagctcagtt ttttaggcaa aaaatcatgt ttcggctcaa
      841 gcacttcaaa caattcttgt ttggacttgt ccggaaacac tgctttcaat gcattgattc
      901 cccgtccgat ttgttttttc agtcgttcta tataggtatc gaaatcaatc atgatcttgc
      961 ttttgaccgc ttcaatacgg tttgcgctgc ctgtaaaccg ttcgcaaatc gggtatgctc
     1021 cgccgaaata ctcgcccggt gtctgtaaga cttcaaaggg aattacaatg tctttcgctt
     1081 tgaattcgat ttcaaagcgc gtccaaatgc ttgttttatc gccaagctgc ttgccttttt
     1141 catagattcg tacatatttt gaagactcac gcgaacctat gccgtaggtt ttgcccctcg
     1201 tcattttggc ttcgtcttct tcctcccagt caaagcctag gcactcgccc ttcggtttaa
     1261 cgtgatggca cgtgaacaaa cctttattgc ggtcttctct tgcctgattc gggctgtact
     1321 ctccgttgaa aaagtctttt gcgatgtcaa cgcgcgttat ttttggacga atcgcatttg
     1381 tcagaaaatc aaaaagcctt gattcccatc cgatatttgc agcattgcag cctaccgccg
     1441 tcaattcaaa aagcatcgtg ttgttttggc cgccgaaatg gacgcgccca tattgggcat
     1501 tttccgttcc catcagccag cagccttgat agaaacgccc gcccgaatgc ttggcttttt
     1561 caatgattcc gaatccaaaa atttcttcca tcttggctga agccgcgcgt atgaaatcgt
     1621 catcatcgaa caaatcaaac gaaagtccat aaacatggaa aaacgtgtct tcatgaattg
     1681 aaaatgtgat ttggtcaatg aaagccgaat ctgatacacc gcgcctaaga ggaacgccta
     1741 acaagtttcc tttgccgtcc gttatgtacg tttcgtaaca ttcgaagact tcctgaaccc
     1801 tgcccgccgt ttcggtttct gtaccccccc tgttagataa ggggggaaga tttgaagcgg
     1861 ttgccggctt cctgccgtcc gctaaagcgt ccgtcatcac gccggcaacc gcctttgtca
     1921 tcccttgctt atcttccatg gtgcgaatcc tcaaaaacgg gcaaaaaaaa aaagccctgt
     1981 tacttgtaga aagtaaagga cgttaatttt tgttaatcgt cccttcttag ggacgcaata
     2041 tataaggttt tagattgcga gaatttatcc gctccgccgt cattcccacg gaagtgggaa
     2101 tccagaaatg aaaagcaaca ggaatttatc gtaaatgact gaaaccgaac ggactagatt
     2161 cccgcctgcg cgggaatgac gatgtaaaat tatccgggat tcaaaaagac agactttcgc
     2221 gtccgtggga atgactgcgg aaagatgatt tttattttgt cataaaaatc cgcaccttaa
     2281 tcagttggcg gttaaatcaa acttttaggg tgcagattac tttttatgat ttcagacagc
     2341 attttgacag acggcaaacc tatttcggca ataccaaaaa cttaatcaat aactctttga
     2401 acacaaaacc gaacacgccc aagcccaaaa ccaaaaacaa aatggcgatg ccgaatttgc
     2461 ccgctttaga ctccttgccc aaattccaaa cgataaaacc caaaaaaata atcaaaccgg
     2521 tcaggcagat tttcaacgcc caatcggcaa aaaccgcttc atccatattt ttttcctatt
     2581 gttgacgtgt atgtcatata agataagggt ttcagacggc atttgcggtg tttttccgcc
     2641 gatgccgtct gaacacgcaa tcagcgcgcg agtgcctgtt tcaaatcgtc aatcaaatcg
     2701 ccaacatatt ccaaaccgac cgacaggcgc accaatccgg ggcggatgtt ggcggcgagt
     2761 ttttcttcgg gctgcatcct gccgtgcgtg gttgtccacg gatgggtaat ggtcgagcgc
     2821 acgtcgccga ggttggcggt acgggagaaa agttcgacgc cgtccacgac tttccacgcg
     2881 gctgcttggt cggcgacttc aaagccgatg acgatgccgc cgccgttttg ctgtttgcgg
     2941 ataagctccg cctgaggatg gtcgggtaat ccggtgtaat acacggcttg aacctgcggc
     3001 tgcgcttgca gccattgcgc gattttcaag gcgttgtcga actgtttttc catacgcaga
     3061 gacagggttt ccacgccgct caacaactgc catgcgttaa acggcgacat tgccagcccg
     3121 caagagttgc agtaaacggc gacctgcgcc atcagttctt ccgaacccgc caacacgccg
     3181 cccatcacgc gcccgtgtcc gtctatggct ttggtcgcgg aggaaacgga aatatccgcg
     3241 ccgtgtttca aaggctgaga gccgacaggc gacagcaggc tgttgtccac caccaagagc
     3301 gcgccgatgc cgtgcgccaa ttccgccaag gcttccaagt cggccacttc tcccaaggga
     3361 ttggacggcg tttccaaaaa cagcagtttg gtgttggctt tgacggcggc tttccattcg
     3421 tttatatcag tcggcgacac gtgactcact tcgatgccga atttggtaac gatgttattg
     3481 ataaagccga cggtcgtgcc gaacaggctg cggctggaaa tcacatggtc gcccgcctgc
     3541 aaaaaggtga aaaacgccgc ctgaatcgcg gacatgccgg tcgaagtggc gaccgcgcgt
     3601 tccgcacctt ccaaagcggc gatgcgtttt tcaaaggcgg ctgtggtcgg gttggcggta
     3661 cgggtataag tgaagccttt gatttttttt gaaaacaaat cggcagcgtg ttgggcgttg
     3721 tcccacatga agctgctggt caggaacaat gcctgattgt gttcgcggta ttcggtttgt
     3781 tctttgccgc cgcgtatggc gagcgtttgc ggatggagtt ttttgctcat cggtgattcc
     3841 tcggttttat ccgttcggca acggagcgtg cgcccgttgt ttaatttata gtggattaaa
     3901 tttaaaccgg tacagcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg
     3961 aagcaccaag tgaatcggtt ccgtactatt tgtactgtct tcggcttcgt cgccttgtcc
     4021 tgatttaaat ttaatccact atattaatat tttgcgcctg ttctatgatg ctttcaagtc
     4081 gggtgagaat gcaaatgccg tctgaaacgg ctttcagacg gcatggcaat cagcgtttgt
     4141 attttaactc gtacttgatg tcgttgagga ttttgcggac atcgtgttcc aacacgtctt
     4201 cgactaccgc acccgcctgc tcgtgcagca tctgctggag ctgataggta aaaaccgcca
     4261 tctgcttttg caccgccgta cggatgatgc cgttgacggt atcggtcaga tgcgggcgca
     4321 ggcgtttgat cagccgttcg gtcagctcct gttcggacag gcagaacact tcgcgccggt
     4381 tgacggcttt cggattcagg atattgattt ggacgggcat caacgcctct tccgcaccgc
     4441 cttcgccgtt ttccgcaacc ccgtctgccg ccgtacccga ttctgccgca tcggcgtttt
     4501 ccccgccttc aatctgtccg gtttcaaatt cggcactgtc ttttttggca tcgaacagga
     4561 ttctccgccg cgattcgatg tgtttttccg aaaccgacat ttgcagggaa gcctgcgcgt
     4621 tgagccagtt ttcctgaagg acaatcatcg ggtcggtttc gacttcctcg ccgcaatcgg
     4681 caacggcggc attgtgttcc tcctgccatt tcttcagata cgcctttaac acacggtttc
     4741 ggcttgcatc gtccagtttc ggcacagacg cgtccgttcc ggtttcagaa gggcgggaca
     4801 gcggcgcgta agtcggcact gccttcatac ggcgcgtctg acgcaggttt tccaaacgtt
     4861 tttcccaatt cggctcttta ttcgcatcca ttttcggctt ccggttctta atctttgcag
     4921 gcagacaaac ccgcgcccaa agcgcggttt gatataatgg cgcattttaa cagattcgcg
     4981 aggatacatc atgggcagca tcgaacagcg tttggaatat ctggaagagg cgaacgacgt
     5041 gctgcgtatg cagaaccacg tcctgtccac cgcattcaaa gccttaatcc gcgcccttcc
     5101 cgccgaaacc gccgaaatcg cggtcgagtc gattcagctt gcttttgagg acgccttggc
     5161 agaattgagc tatgaggaca gcccccatac cgatttgttc cacgacgtta cttatgcgtt
     5221 tttccgtgaa aaagaacgtt aattttgtgt taaactgatt ttttaggctt tttgattacc
     5281 gaaaggaatt ttgatgaata tgaaaaaatg gattgccgcc gcccttgcct gttccgcact
     5341 cgcgctgtct gcctgcggcg gtcagggcaa agatgccgcc gcgcccgccg caaaccccga
     5401 caaagtgtac cgcgtggctt ccaacgccga gtttgccccc tttgaatctt tagactcgaa
     5461 aggcaatgtc gaaggtttcg atgtggattt gatgaacgcg atggcgaagg cgggcaattt
     5521 taaaatcgaa ttcaaacacc agccgtggga cagccttttc cccgccttga acaacggcga
     5581 tgcggacgtt gtgatgtcgg gcgtaaccat taccgacgac cgcaaacagt ctatggactt
     5641 cagcgacccg tattttgaaa tcacccaagt cgtcctcgtt ccgaaaggca aaaaaatatc
     5701 ttcttccgac gatttgaaaa acatgaacaa agtcggcgtg gtaaccggct acacgggcga
     5761 tttctccgta tccaaactct tgggcaacga caacccgaaa atcgcgcgct ttgaaaacgt
     5821 tcccctgatt atcaaagaac tggaaaacgg cggcttggat tccgtagtca gcgacagcgc
     5881 ggtcatcgcc aattatgtga aaaacaatcc gaccaaaggg atggacttcg ttaccctgcc
     5941 cgacttcacc accgaacact acggcatcgc ggtacgcaaa ggcgacgaag caaccgtcaa
     6001 aatgctgaac gatgcgttgg aaaaagtacg cgaaagcggc gaatacgaca agatttacgc
     6061 caaatatttt gcaaaagaag acggacaggc cgcaaaataa gcccgcccgt ccgaacacaa
     6121 tgccgtctga agccctttca gacggcattg ttcatcaatc ggcctacaat gaactgcctg
     6181 ctgatttctc cctaccgcaa agcaacaggc aaagattaca aatatcaaaa tccgagtaaa
     6241 acagtatttt attaaaacaa attgataatc aagagattag aattatgtat tgtctttacc
     6301 gtacaaacgc tggcactatt tcaacctgat aaaaaacagc cttcaaaaag gttgcttaaa
     6361 acagcagcag acacttaccg ccacaacctt gaaaaggaac acaatcatga ccgtcatcaa
     6421 gcaagaagac tttatccaaa gtatctgcga tgccttccaa ttcatcagct actaccatcc
     6481 gaaagactac atcgacgcgc tttataaggc gtggcagaag gaagaaaatc ccgccgccaa
     6541 agacgcgatg acgcagattt tggtcaacag ccgtatgtgt gcggaaaaca accgccccat
     6601 ctgccaagat accggtatcg caaccgtgtt tttgaaagtc ggtatggatg tgcagtggga
     6661 tgcggatatg agcgtggaag aaatggttaa cgaaggcgtg cgccgcgcct acacttggga
     6721 aggcaatacg ctgcgcgctt ccgttctcgc cgaccccgcc ggcaaacgcc aaaataccaa
     6781 agacaacacg cccgccgtca tccatatgag catcgtgccg ggcgacaaag tcgaagtaac
     6841 ctgcgcggca aaaggcggcg gctctgaaaa caaatccaaa ctcgccatgc tcaatccttc
     6901 cgacaacatc gtcgattggg tattgaaaac catcccgacc atgggcgcgg gctggtgtcc
     6961 tcccggcatc ttgggcatcg gcatcggcgg tacgcccgaa aaagccgtgt tgatggcgaa
     7021 agaatccctg atgagccaca tcgacatcca agaattgcag gaaaaagccg cgtccggcgc
     7081 ggaattgtcc accaccgaag ccctgcgcct cgaactcttt gaaaaagtca acgcgctagg
     7141 catcggcgcg caaggcttgg gcggtctgac caccgtgttg gacgtgaaaa tcctcgatta
     7201 cccgacccac gccgcctcca aaccgattgc catgattccg aactgcgccg ccacccgcca
     7261 cgtcgaattt gaattggacg gctcaggccc tgtcgaactc acgccgccgc gcgtcgaaga
     7321 ctggcccgat ttgacttaca gccccgacaa cggcaaacgc gtcgatgtcg acaagctgac
     7381 caaagaagaa gtggcaagct ggaaaaccgg cgacgtattg ctgttgaacg gcaaaatcct
     7441 caccggccgc gatgccgcac acaaacgcct cgtcgatatg ctcaacaaag gcgaagaatt
     7501 gcccgtcgat ttcaccaacc gcctgattta ctacgtcggc cccgtcgatc cggtcggcga
     7561 tgaagtcgtc ggtccggcag gtccgaccac agccacccgc atggacaaat tcacccgcca
     7621 aatgctcgaa caaaccgacc tcttgggcat gatcggcaaa tccgagcgcg gcgtggccac
     7681 ctgcgaagcc atcgccgaca acaaagccgt gtacctcatg gcagtcggcg gcgcggcgta
     7741 tctcgtggca aaagccatca aatcttccaa agtcttggcg ttccccgaat tgggcatgga
     7801 agccatttac gaatttgaag tcaaagacat gcccgtaacc gtcgccgtag acagcaaagg
     7861 cgaatccatc cacgccaccg ccccgcccca atggcaggcg aaaatcggca tcatccccgt
     7921 caaatcttga ggcgtcatgc cgtctgaaca caaaatctgc tttcagacgg catttccgcc
     7981 cctggttgcg gtacaatcca ccatttcatc actcggcgac ccacaccgtg aaaatcctca
     8041 ttttaggcaa cggacaggta ggttctaccg tcgcacaaaa ccttgccgcc atacccaaca
     8101 acgacgtaac cgttatcgac atcgacgaaa aagcattgca ggaaacaggc agccgcctcg
     8161 atgtccaaac cgttttcggc aacggcgcat cccccttcac attagaacgc gccggcgcgg
     8221 aagatgccga cttgctgctc gcgctctccc gcagcgacga aaccaacatc gtcgcctgca
     8281 aagttgccgc cgacctgttc aacatccccg gccgcatcgc gcgcgtccgt tccagcgaat
     8341 acctcgaata cctcagcccc aagctcgaaa acaacgaaaa cggcagcctt tccatattcg
     8401 gcataaccga aaccatcagc cccgaacagc tcgttaccga acagcttgcc ggcctgatag
     8461 actgcccggg cgcattgcag gttttacgtt ttgcagacga ccgcgtgcgg atggtcatca
     8521 tacaggcgcg gcgcggcgga ctgcttgtcg gacgcagcat tgccgacatc gcccaagatt
     8581 tgcccgacgg ggccgactgc caaatctgcg ccgtttaccg caacaaccgc ctcatcgtcc
     8641 ccgcgccgca aaccgtcatc atcgaaggcg acgaaatcct atttgccgcc gccgccgaaa
     8701 acatcggcgc ggtcataccc gaattgcgcc ccaaagaaac cagcacccgc cgcatcatga
     8761 ttgccggcgg cggcaacatc ggctaccgtc tcgccaagca gctcgaacac gcatacaacg
     8821 tcaaaatcat cgaatgccgg ccgcgccgtg ccgaatggat agccgaaaac ctcgacaaca
     8881 ccctcgtcct gcaaggttcg gcaaccgacg aaaccctgct cgacaacgaa tacatcgacg
     8941 aaatcgacgt attctgcgcc ctgaccaacg acgacgaaag caacattatg tccgcccttt
     9001 tggcgaaaaa cctcggcgcg aagcgcgtca tcggcatcgt caaccgctca agctacgtcg
     9061 atttgctcga aggcaacaaa atcgacatcg tcgtctcccc ccacctcatc accatcggct
     9121 cgatactcgc ccacatccgg cgcggcgaca tcgttgccgt ccaccccatc cggcgcggca
     9181 cggcggaagc catcgaagtc gtcgcacacg gcgacaaaaa aacttccgcc atcatcggca
     9241 ggcgcatcag cggcatcaaa tggcccgaag gctgccacat tgccgccgtc gtccgcgccg
     9301 gaaccggcga aaccattatg ggacaccata ccgaaaccgt catccaagac ggcgaccaca
     9361 tcatcttttt cgtctcgcgc cggcgcatcc tgaacgaact ggaaaaactc atccaggtca
     9421 aaatgggctt tttcggataa accgccccat tccggacata ttgccgccaa gcggtatgga
     9481 agcagaaata atggtaggtg ggcttcagac ggcatccgcc ctccccgtca ttcccgcgta
     9541 agcgggaatc cagaccttgg gatagcggca atattcaaag gttataaaag acccgtcatt
     9601 cccgcgcagg cgggaatcta gaccttggga tagcggcaat attcaaaggt tagctgaagc
     9661 tttagagatt ctagattccc gtttccacgg gaatgacgaa aggttgcggg aatccaaacc
     9721 attgggcaac agcaatattc aaag
//
LOCUS       DAWFJW010000071         9554 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFJW010000071 DAWFJW010000000
VERSION     DAWFJW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.071"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WGR19_001419"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="WGR19_001419"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0798929.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPKQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(3151..3486)
                     /locus_tag="WGR19_001420"
     CDS             complement(3151..3486)
                     /locus_tag="WGR19_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798930.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKSAAY
                     AILKERGEV"
     gene            complement(3500..3973)
                     /locus_tag="WGR19_001421"
     CDS             complement(3500..3973)
                     /locus_tag="WGR19_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0798931.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDIFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4026..4307)
                     /locus_tag="WGR19_001422"
     CDS             complement(4026..4307)
                     /locus_tag="WGR19_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798932.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKMDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4394..4717)
                     /locus_tag="WGR19_001423"
     CDS             complement(4394..4717)
                     /locus_tag="WGR19_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798933.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(4856..5035)
                     /locus_tag="WGR19_001424"
     CDS             complement(4856..5035)
                     /locus_tag="WGR19_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798934.1"
                     /translation="MPSERTFCRSDGIFISFFRLPPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            complement(5051..6058)
                     /locus_tag="WGR19_001425"
     CDS             complement(5051..6058)
                     /locus_tag="WGR19_001425"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0798935.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDSADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     Q"
     gene            6290..6526
                     /gene="acpP"
                     /locus_tag="WGR19_001426"
     CDS             6290..6526
                     /gene="acpP"
                     /locus_tag="WGR19_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0798936.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6682..7929
                     /gene="fabF"
                     /locus_tag="WGR19_001427"
     CDS             6682..7929
                     /gene="fabF"
                     /locus_tag="WGR19_001427"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0798937.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            8284..9417
                     /locus_tag="WGR19_001428"
     CDS             8284..9417
                     /locus_tag="WGR19_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0798938.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRAGGGGDLERLAARDIIRFPGFVNNVSEVIKE
                     HHIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILA
                     EKMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT         2275 a         2515 c         2429 g         2335 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tggataaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acttcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcgataggcg gtgcggcttt
      421 gtccggcgag ggttttgtca atcaaaccgc agtcggcgga gatgtttaag agggcgatgt
      481 tgccgtagtt gtccaagagt tgcggatact ggcgggcatg ggcaagtatc agatattgga
      541 cgataaattc gacgtcgacc acgccgccgc gtgcgtattt gacgttgctg tcggcaggcg
      601 gatgggtggg gaacattttt tcgcgcattt cgatgatttc gcctgacaag gcggtttggt
      661 cgcgttcggc ggtgaggatt tcggtgcgga tgcggtcgaa ggccgtctga atttcggacg
      721 tgccgcagat gaagcgggcg cgggtaaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcg gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccagt ggcggcggaa agccagttgg
      901 tcaggcggcg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtggggg tcgtcgtaga
      961 ggtagacgag gtcgaggtcg gaggcgtagc cgagttcttt accgccgagt ttgccgtagc
     1021 cgacgacggc gaattgcggt gtgtcgcggt gttttttggg catgtccgcc catgcgcaca
     1081 gcagggcggc ggcgaggatg gtgtcggcga gggcggagag ttggtcggag agggattcta
     1141 ccgtccacag tccggcgagg tcttggacgg cgagacggaa gacttgggcg tgctggaagc
     1201 ggcgcagggt gtccatttgc gcttcagtat cgccgccgca ggctttgagg tcgtctgaaa
     1261 gggcggcggc gagcgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctgcgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gcgctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggcttgtcct
     1681 gccatgccca ttgccattcg ctgttgtctt gcgtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttgttccgat gaacattgag accgtctgaa aaagcggaat
     1801 aactgtcgaa acccatgctt tcggcgagca gttgccgctg tttgggcgag gtcggcaggg
     1861 tttgggtttg ctggtcatcc cagtattgca ggcggtgttc gacatcgcgc aggaatcggt
     1921 aggcggcaag cagggtttcg acgttttcag acggcatgat cccggtttcg gcaagtttct
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcacgcat ttggccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaat tcgacttcgc ggatgccgcc cgcgccgagc ttgatgttgt
     2101 ccgccatgcc ttttttgctg acttcgctgc ggatttggcg gtgcagctta cgcattgcct
     2161 catacgcgcc gtaatccaga tatttgcgga acacaaaggg gcgcaccagc gatttgattc
     2221 cgttcggata cggcgtaacc acgcggcctt tgcaccacgc gtagcgttcc cattcgcgcc
     2281 cctgcgtaat caaatattgc tccagcgcgg tttcgctcaa taccaacgcg cccgaatcac
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgccc gtcggcggta atgtcgttca
     2401 gcagcgcaat cagtttctgc ccgactttgg tgaaaaactc ttgattgccc cgttcgcgcc
     2461 tgccgtcggt gtcgcccgat tcgggataga cgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacgct caaatgctgc ggcgatttgg
     2581 tataacgccc gatcggcgtg ccgtacatgt cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatgcggtt tatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caattcttcc tcgttttctt ccgcacggat tttgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aaaaccttgt ccagcatagg caggaaaatt tcgggcttga
     2881 gtttgccgtt gtcgagctgg cgggcgagga agagggaatg gcggcgggcg gtgtcgaggc
     2941 ggttgtcgga catttcggat tccgtttgaa aaaatggcag gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg taccggttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgccgtct gaaaccttgc cccgacatcc agacggatgc ctgccgataa
     3121 aaaacagcgg caaatgccgc cgtttcctgt tcacacctcg ccacgctctt ttaaaatagc
     3181 ataagccgca cttttctccc gtgtggaatt tttaaaaaaa ccatcactct tgacgatttc
     3241 atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat gctcattgcc
     3301 ggcttcaatc attccctctc ccactgcttt tgctgctttt gccacaccat tccaaaaacc
     3361 cataatcatg tcctccaatc gtttcaaata aataaggtcg ttgtttctgt caagaaacaa
     3421 cgacagttta taaactgtcg gaaaacaaaa tgccgtctga aggccggata atgcttcaga
     3481 cggcatcgga ttgccggttt taaccctgcc ctccgccgtt gttcaaatag tgttcccagt
     3541 gagaatataa ccgggcaata ttttcttcat aggtgtgggc agaaggaata taaccgtctt
     3601 tgggcggcat atacctgaca aatgattcta tttcttccgg ataatcaaaa tctgcataaa
     3661 ttttttcaac ttcccccaaa ggatcctcaa attcttcttt tctatggaat atatcgctta
     3721 agaggacgta cagccaattt ttaggggaat aatccctcat ttccgaagcc aaatcttcca
     3781 gaacaggctt taattcaaga atattggatt tgttgattaa agatagtttg aacacacgtt
     3841 catcatgatc tgaaagcgtc atttttcggg cataagctgc cacatcagcc caacccaagt
     3901 atttattccc atacccccac agaatatcct tccaagaaag acggactttt tgcgcggtta
     3961 aatcaaggtt cattttttac tccggttgcc tgccccgtca ttccgcccct tccgattgac
     4021 gcctgttacg aaaagtcttc caaatgacgg ctgattgcct cttcaacagt cggatgccaa
     4081 tccataaatg aagcctgcaa ttcctctaaa accaactcat tgaataaatc atccaaattc
     4141 cgccaatcca ttttttcatc cggatagatg aaatttgcca taacgacagt atgtttgttt
     4201 tgggaataat ccgataaggc gcaaatcatc agataccgcc ccgaatgctc cagataaaaa
     4261 attcgttttt ccaaaggaaa ccggttattc cgttgcccgt tttccatttt tcatcctttt
     4321 taacgtatcg gcttccgcgt ccttcaaatt gagttacctg ccttccgcca agcaaacgcc
     4381 cgcgcccctt tcatcatttg aatatccgtt tgaccatatt taccagataa ctatcttctt
     4441 tatataaatc aatcagcaaa ggcacgatac ccggctcata aaataattct gtcgtatcag
     4501 atgtttcttt taacgcggaa attatctcca acagttcata cccgtatcgg ggataagcaa
     4561 acaaataggg gactgcttcc cgcaaacatg aaaatgcttc gtaactttgc atttcccata
     4621 tatgttcatc tttaaaatca ctcaaatacc attcatccaa ttcactttcc ggatcggata
     4681 tcaatcggat aaattcattt agttcgtcct taaacatatc aaatccttaa atataaaatc
     4741 ttttgattat ccgccgtatc cttattttcc acatcatgaa tttcaacgcc gttcatattt
     4801 ttcgttttct caaaccgtcc accacggcat aaaattattt tttcatatca attatttata
     4861 tgtttaacaa cttaccgccg gacttgcttt ggctgcgtat caagattgtc cggttcgcgg
     4921 cgggcgtttg ctttgtttca aacagggcgg cgcgtaaggg attttcaagg ggcggcaggc
     4981 ggaaaaagga aataaaaatg ccgtctgaac ggcaaaacgt gcgttcagac ggcattttgt
     5041 gcgggacgcg tcattgcgcc aaagccttca aacattcttt gaccaatgcc ggacctttgt
     5101 atatcaatcc gctgtacacc tggacggcag ttgcgcccaa gcggatttta tctgccgagt
     5161 ccccgccttc cataatgccg cctacgccga taatcggcag cttgccgtcg atgtggtctg
     5221 ccaacagctt caacacccga ttactttttt catgaacggg cagcccgctc aaaccgccct
     5281 gctcgcctgc gagcggatgg ctgccgagac ttgatttgtc gatggtggta ttggtagcga
     5341 tgatgccgtc catttcgacg gatttgacaa cgtgggcgat gtcttcgatt tgtgcttcat
     5401 ccaaatcggg ggcgattttg acggcgagcg gaacgtattt cccgtataca gaggcaagct
     5461 gtgcctgttt gtttttcaaa gcctcaagca atgcgctcaa ctcgtcgcca ccttgcagcg
     5521 cgcggaggtt tttggtgttg ggcgaggaaa tgttgacggt aatgtagctt gcgtgcgcgt
     5581 aggctttttc aaggcagatt aaataatcat cggcagcgtt ttcgatgggt gtaaccgcgt
     5641 ttttaccgat gttgatgccc aatacgccac tgaatttact tttttcgatg ttgcgtatca
     5701 tggtgtcgat accgtggttg ttgaaaccca tgcggttgat gatgccttgg tgttcgggaa
     5761 cgcggaagag gcgcggctgc gggttgccgg gctgcggttt gggcgttacc gtgccgattt
     5821 cgatgaaacc aaagccgagc gcgcccaatg cgtcgatgta ttcgccgttt ttgtcgagtc
     5881 cagcggcaag tccgacaggg ttgggcaaat ccatacccat caattttaca ggtttggtac
     5941 ggttgccggt tacgggaatc agacccaatt tataaaccgt gtagagcgcg tcgagcgtga
     6001 agtggtgggc tttttcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga
     6061 cagggctttc tgcttcagat ggaaatgccg ccattttaac cgaaatcccg ccaaacatac
     6121 ggcacacagg tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatcggca
     6181 aatattgata taatagtcat tagatagaaa actctattaa aagtcggtac aataccaccg
     6241 atttatttcc cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat
     6301 cgaacaacaa gttaaaaaaa ttgttgctga acaactgggc gtaaacgaag ccgacgtgaa
     6361 aaacgaatct tctttccaag acgacttggg cgcggattct ttggacaccg tggagctggt
     6421 tatggctttg gaagaagcat tcggctgcga aatccccgac gaagatgccg aaaaaatcac
     6481 caccgtccaa ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg
     6541 acacaacagc ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt
     6601 gaacccctgc cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg
     6661 acaaccaaca gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag
     6721 gtttcccctg tcggcaacac tgtcgcagag gcttgggaca ccctgctcgc cggcaaaagc
     6781 ggcatcggcg cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa
     6841 gtgcgcggtt tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgta
     6901 ttcatccact acggcattgc cgccgcattg caggcaatcg ccgattcggg cttggacgat
     6961 gtggaaaacc tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg
     7021 cccagcatcg aggtcaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct
     7081 ttctttatcc ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc
     7141 taccgcggcc cgagctacgg gatggtttcc gcctgtacca ccggcgcgca cgccatcggc
     7201 aattccgccc gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa
     7261 ggcgcaatca gcactttggg cgtgggcggt tttgccgcga tgaaagccct ctccacccgc
     7321 aacgacgacc ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgttatc
     7381 ggcgaaggcg cgggcatatt ggtgttggaa gaattggaac acgccaaaaa acgcggcgcg
     7441 aaaatctacg ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg
     7501 ccgaacgaag aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat
     7561 cccgaagacg tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac
     7621 gaaaccaaag ccctcaaacg cgcgttcggc gaacacgcct acaaaaccgt cgtcagctcg
     7681 accaaatcca tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc
     7741 attttggcga tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt
     7801 gaagccggct gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt
     7861 gccatttcca actccttcgg cttcggcggc accaacggca cgctggtctt caaacgcttc
     7921 aaaggctgat tccgcaaagt cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac
     7981 ggcattttca aaacaccccg cccgttaccg gcacttcgcg gcacacaagg cagccttata
     8041 atacgcaggc aaacccaagc cctgccggat tgcgggacga aaccctgccg tagcagaccg
     8101 tatttccaat catatattca aggtgtccgc cttatgtttc aacacacagg acgacacata
     8161 aagcaccgcc ctatgtgtcg tcctgatttg gaaggggtta cgccccctcc caaatagagt
     8221 ctgattctac cgccccgaag gacagatgtc cgagtggcgg ggttcaaccg aaaaggaaac
     8281 acgatgaaaa tcgtttttat cacaacagtc gcatccagca tttacggttt ccgcgccccc
     8341 gtcattaaaa aattaatcgg caaaaaccat caggtgtatg cctttgtatc ggagttttcc
     8401 gataatgaat tggatattat cagggaaatg ggggttacac ccgttaccta ccgttcaaac
     8461 cgcagcgggc tgaacccgtt ttccgatata aaatccacct tcctcatctt taaagaactc
     8521 aaaaaaatat cgccggattt ggttttccct tatttcgcaa aacccgtgat tttcggcact
     8581 tttgccgcaa aactggcagg cgtgcccaga atcgtcggga tgctggaagg tttgggattc
     8641 gcatttaccc cgcagccgga aggcataccg ttaaaaacaa aaatcataaa ggggattttg
     8701 attgccttgt accacattgc cctgccgatg ttggaaagcc tgattgtatt aaaccccgac
     8761 gacaaagacg aactgacgga caaatacggc atcaaaataa aaaacatcca tattttgggc
     8821 ggaatcggtc tggatttgcg gcaatatcct tattccgagg cggatattcc cgatgaaaaa
     8881 gaacccgtaa aattcctctt tatcggcaga tttctgaaag aaaaggggat tgatgatttt
     8941 attcgggcgg cggaacaggt taaggacaaa taccccgata cggtttttac cgctttgggc
     9001 gcaatcgaca aatcacgcgc gggggggggg ggcgatttgg aacgccttgc cgcccgcgat
     9061 attatccgtt tccccggttt tgtgaacaat gtttccgaag tgataaaaga acatcatata
     9121 ttcgtattgc cgtcttatta tagggaaggc gttccccgaa gcactcagga ggcaatggcc
     9181 gtcggcaggg cagtgattac gacggatgtc cccggatgca gggaaacggt cgccgacaag
     9241 gtcaacggct tcctgatcga gccttggaat ccccgcatct tggccgaaaa aatgatttat
     9301 tttatcgaaa acagggaagc cgtccgcctg atggggaatg caagttatgc gattgccaaa
     9361 gataaattcg atgccgaaaa agtcgatttg aaattgctcg atattttgaa ggcgtaaaca
     9421 aggctgcccg cttttgagtt tcggtcattt ctgataaacc cccgtcattc ccgccacacc
     9481 cccgtcattc ccgcgaaagc gggaattcag gttcgtttag tttcggtcat ttccgataaa
     9541 ttcttgcaac tttg
//
LOCUS       DAWFJW010000072         9509 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFJW010000072 DAWFJW010000000
VERSION     DAWFJW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.072"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(18..311)
                     /locus_tag="WGR19_001429"
     CDS             complement(18..311)
                     /locus_tag="WGR19_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798939.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITGHNGKPDTKPNTAAKM
                     PSEAVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
     gene            complement(308..1363)
                     /locus_tag="WGR19_001430"
     CDS             complement(308..1363)
                     /locus_tag="WGR19_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0798940.1"
                     /translation="MKTYAPVLYTAALLTACSPTADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1470..1952)
                     /locus_tag="WGR19_001431"
     CDS             complement(1470..1952)
                     /locus_tag="WGR19_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0798941.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1999..3135)
                     /locus_tag="WGR19_001432"
     CDS             complement(1999..3135)
                     /locus_tag="WGR19_001432"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0798942.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFTEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGSEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            complement(3132..3677)
                     /locus_tag="WGR19_001433"
     CDS             complement(3132..3677)
                     /locus_tag="WGR19_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0798943.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3674..5149)
                     /locus_tag="WGR19_001434"
     CDS             complement(3674..5149)
                     /locus_tag="WGR19_001434"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0798944.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGITNMNILAAT
                     FPAGTLSGAPKIRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5260..5625)
                     /locus_tag="WGR19_001435"
     CDS             complement(5260..5625)
                     /locus_tag="WGR19_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798945.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5646..7556)
                     /locus_tag="WGR19_001436"
     CDS             complement(5646..7556)
                     /locus_tag="WGR19_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0798946.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELSLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLAVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7575..8219)
                     /locus_tag="WGR19_001437"
     CDS             complement(7575..8219)
                     /locus_tag="WGR19_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0798947.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8384..9202)
                     /gene="opc"
                     /locus_tag="WGR19_001438"
                     /gene_synonym="opcA"
     CDS             complement(8384..9202)
                     /gene="opc"
                     /locus_tag="WGR19_001438"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0798948.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRANIPFDASQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2102 a         2100 c         2668 g         2639 t
ORIGIN      
        1 cgctgtactg gtttttgtta atccactata aatgttccga tacgaaccgc aaaatattgg
       61 ttttgtttct gacaggcaaa agcactgttt atttggctgt caaaaggatg gttaaggaaa
      121 gttatgcgcc cctgaagcgg gccccagata aggatggttg cgccgacggc ttcagacggc
      181 attttggcgg cggtgttggg ttttgtatcc ggtttgccgt tgtgtcctgt gatgatgatt
      241 ttgggcgcgg tttttctgtt ttgatgtgtg aaatgccgtc tgaaaggcgg ttcagacggc
      301 atagcggtca tttttgtgcg gtcaggcggt cgaatatgcc gccgtcggcg aagtaggttt
      361 tcatgatgtt gtcccatccg ccgaattttt cttcgggaga gaaggtgtct aagtctggga
      421 agtcggcttt gtgtcttgcc aatacttcgg ggttgcgggg gcgcaggtag agtgaggcgg
      481 cgagttcttg cgccggttcg ctccaaaggt attcgagata ggcgcgggcg gttttttgcg
      541 tgcctttttt cgcgacgacg ctgttgacga cggcgacggg gctttcggcg gaaatggtgt
      601 agctcggata gacgatttca aactgtcctt gggtcagttt tttgctgacg tagttggctt
      661 cgttttcaaa agtgatgagt acgtcgccga tgttgcgttg tgtgaaggtg gtggtggcgg
      721 cgcgtccgcc gttttcaaaa acgggggtgt ttttgaggat ggatgcgacg agtttttggg
      781 cttcctgttc gttgccgttg gtggttttca gaccgtaacc gtatgcgccg aggaaggcgt
      841 agcgtccgtt gcccgaggtt ttgggattgg cgatgacgat gttaacgccg tctttggcaa
      901 ggtcgttcca atcgcggatc tgtttggggt tgttttttcg gacaaggaaa accatagtgc
      961 tggtgtaggg cgcggcgtgg tcggggaggg cttgttgcca gcctttttct accagtcctt
     1021 ttttttcgag caggtcgatg tcggaggatt ggttcatggt tacgacatcg gcttgaaggc
     1081 cgttggctac ggataatgcc tgtttgctgg agccgccgtg ggactgttgg atgctgacgg
     1141 atgtgccggg gtgttcggat tggtatgttt tgataaataa ggggttgtat tctttgtaaa
     1201 aatcccgtgc cacatcgtat gaggcgttga gcagggtaat gttttttccg tcggattcgg
     1261 tattggccgg ggcattttgt ccggacggat ggtttgaatc ggctgtgggg ctgcaggcgg
     1321 tgagcagggc tgcggtatag agtaccggtg cgtaggtttt catatgcttg tcctgtcggt
     1381 tggtagatgg ggcaacttta atacggctgt ctgcgcttgt ggaaataatg tttgatttga
     1441 agattatcag ttttggttat aaggacggat cagaggtgtt tccgcatcag ttcgcatttg
     1501 attttgatgc tggggtcaag ctgcaatact gccgaaccga gcgattcgta gcggttgaga
     1561 aggtaaaacg ggacggagtt gagcgatgcg tagagtttca gaaagctcaa accggatttg
     1621 tgggcgatgg tttccgcctg atgaagcagg gcagtgccca gtccgaggtt gtggaacagg
     1681 gggtggacgt aaagtgcatc gagttgtgct tcttggcagt cgatttggaa aaatccctgt
     1741 atgttgcctt tgtattcggc aacccaaagt gctttgtcgg gatcggaaat ggtcggcagg
     1801 tagctttctg tgtttaacaa gccttcccat acttttaggg cgtgttcgtt gtagctgagg
     1861 atgcaggtgt attggacgga gtgcaggtgg actttgaaga tgtctttgca gtcttgcacg
     1921 gtggcggggc gtaacagtgt cagcaggctc atggcggtat gtcggcggct tcagacggca
     1981 tctgtgccgt tggtcggatt atagggactg atgcagtttt tttgcttctt gaaatgcggt
     2041 gtccgaatcg gtggttaaaa cggtaaagtg tcccattttc cgccctttgt gcgcggtttt
     2101 tttgccgtaa aggtgcaggt gcgcgtccgg ccggctttgc aggggaaacc aatccggttc
     2161 gctgccgtct tcctgccaaa cgtcgcccaa aatattcgcc atacagcaag aactcagcaa
     2221 tttggtgtca gcaggtggca ggttgcacat caggcggacc tgttgctgga attggtctgc
     2281 cgcgcaggcg tcgacggtat ggtggccgga attgtgcgga cgcggcgcga tttcgttgac
     2341 gaccaattca tgcgtgtcgc cgacaacaaa catttctacc gccaatacgc cgacgtagtt
     2401 caattcatcg gccaaacgct gcgccatttg tcgcgcctgt tgctgaatgt cggcactcag
     2461 tcgggctggg acgatggagt aggcgaggat accgttttcg tgaatgtttt cggcaggatc
     2521 gaaagtttgc acgttgtcat tgttcaaacg gcatacaatt acggaaatct cactgcgcaa
     2581 atccaccatt ttttccaaaa cgcaatccac gccgccgtgt tcggtaaacg cggctttgag
     2641 ttcatccaat gtttttacgc ggatttgacc tttgccgtcg tagcccaacg tagccgtttt
     2701 caggatgccg ggcaaaaatt gcgcgcttgc ttcagtgatg tcttcagcct tacaaaccac
     2761 ttgatacggc gcggtttgca atcccgcttt gcgtatccat gccttttcct gaatgcggtt
     2821 ttgtgcaatc gccacacaat cgccgctagg ggaaacattg gtatgttttg ccaaaaagcg
     2881 catcgcatcg gcattgacgt tttcaaattc agtggtcacc gccgcgcatt ttgccaattc
     2941 atccaaagcc gcttggtcgt taaacggcgc gcataaatga cagtcggcaa attctgctgc
     3001 cggcgcgtcc ggatcggggt cgagaacggt tactttgtag cccatggttt tggcagcaac
     3061 agtaaacatt ctgcctaatt gtccgccgcc aagaatgccg agcatggcgg gcggagaaag
     3121 agatatgttt ttcatgctga ctcttcaaat tgtacaagtt gatagctata actaattctt
     3181 gacggatgtc ttgtatcgct ggaattacca gtttcagaaa tacagaatac tttttccata
     3241 aatttttctg cttttagaaa ttccagtatt ctgttttttt catccttata agcactgcgg
     3301 tctgtacccc atgcaagaat aatcatatca gcatcttcca aacatccttt aaatttggaa
     3361 aaatcggttt gggtgtttgc cctaattcct gtttgctgtg tagagtaact ggaaaaaata
     3421 tttaacattt tgaagttggt aaaaccgtac atatccaaga aacgtgcaag ttgggtaagg
     3481 gttttgtcgc ttctttcatc atttgcttta cttggattaa ttcctatagc aacagctgag
     3541 aaatttttag gattttctgt gttgccgctc catctaaccg ttaggatttc tcgacttttt
     3601 tcattatctg tatagagacc gtctttggta gtcggtctga gtgtttggcg aagctcataa
     3661 agtttttcat aagtcattta tccaaccctt cctgcaccat ctgcgcggcg cgtataacgg
     3721 ctcgggcttt gttttgtgtt tcctgccatt cggaggccgg atcggagtcg gcaaccacgc
     3781 ccgcgccgct ttggacgtat agcgtgttgt tttttactac ggcggtgcgg atggcgattg
     3841 ccaaatccat gtcgttgttg aagccccata cgccgacggc gccgccgtag atgccgcgtt
     3901 tgctcggttc gacttcttcg atgatttcca tagcgcggat tttgggtgca cctgaaagtg
     3961 tgccggcggg gaaggtggcg gcgaggatgt tcatgttagt gatgccgtct ttcagacggc
     4021 cttcgacgtt ggaaacgatg tgcatcacat gggagtattt ttcaatcacc attttgtcgg
     4081 ttactttgac ttcgcccgtt ttgctgatgc ggccgacatc gttgcgtcct aaatcgataa
     4141 gcatgacgtg ttctgcgatt tctttggcgt cgcttaataa gtcttgttca ttggcaaggt
     4201 cttcggcggg ggttttgccg cgcaggcgcg tgccggcgat ggggcggacg atgacatcgt
     4261 cgcgttcgcg gcggacgagg atttcaggcg aggagccgac gatgtggaaa tcgccgaaat
     4321 cgtagtaaaa gaggtaaggc gaaggattga gcgtgcgcaa ggcgcggtag agggcgagcg
     4381 ggctgtcggt gaattccatg ctcatgcgct ggctggggac aacctgcatg cagtcgcctg
     4441 caaagatgta gtctttgatt ttatcgacgc aggctttgaa cggctcttcg ccaaattcgc
     4501 tgacggcttc ggtgtgtttg ctgccgagcg agagcgggat ggcgcagctt tggcgcaact
     4561 gggtgcggat gtcttcgagg cgttcgcggg cgcgttcgta gccgtcgggc tgcgacggat
     4621 cggcgtaaac gacgagatgg attttgccgc tcaaattgtc gatcaccgcc aattcttgcg
     4681 acagcatcag caagatgtcg ggcgtgccga gcgggtcggc tttggtggtg tttttcaggc
     4741 ggtgggcgaa gtgttcaaaa ttgtagatgg tttcgtaacc gaagtagccg accagtccgc
     4801 cggtaaagcg cggcaggctt gggatttcgg gtgttttgaa gcggttgtgg aaggcttcga
     4861 taaagggcag ggggttgccg tcgtgttgct cgacaatttc gccgttttga taaacatcga
     4921 cgtgtttgcc gctggctttg agatagtggc tgcaaggcag gccgataaaa gaatagcggc
     4981 cgaaacgttc gccaccgaca acggattcga gcaggtaggt ataggggcgg ttggcgagtt
     5041 tgagatagag ggaaagcggc gtatccaaat cggcaaggag ttcttgcacg agcgggatgc
     5101 ggttgtagcc ttgggcggct tgggcttggt attcttgttt gctgatcatt tctgctttcc
     5161 caaagggcgg tttcggacgg cgcggcaacg ggcgcgagta tagcatttta tcggaattgt
     5221 tgacagtctg accggagatg ccgtctgaaa aagacgggct tacggctttg ttttgcgctg
     5281 ccggtattgt ttccatttaa acagataacc tgcggttacg ggaatcaggg ctaagatggt
     5341 tttgaacgtc catgatgcat accaaggtaa agaaccgggt gcgaccgatt gattcagata
     5401 cggccaccaa caggcagtca ggcagccgag cagcagcggc cagatatgcc ggctgaggtt
     5461 tttttgccaa aggaggaatg atgcggcaga ccaaagcagg gtaagggcag tgatttttaa
     5521 cacggtataa gcgtccgctc caaacagggc ggcgagaaaa tacgtaccgg caatccagag
     5581 gacggcaagt atcaggacga tgatttcttc aggtcgtttg aacatttttt tcttcctgtt
     5641 tgatttcaga cggcattgcc gttctgtttg gtttccagca gctcccagcg ttccagcttt
     5701 tccaaaagca gcatttcgat ttcttcggcg cggctttgca gtgcgccggc tttttcgtaa
     5761 tctttgaaga tttcgggatc ggaaagctgc gcgttgattt ctgcctgttc ggcttccaag
     5821 gcggcgattt cgtcgggcag ggcatcgagt tctcgctgtt ctttgtagga cagtttgacc
     5881 gtgcggttgg ctttgggttt ttctttcacc ggttcggcat cggctgtttt gggtgcggat
     5941 tccgtctgaa tcttcgcttc gcgtgatttt gcgtcgatgt agtcctgata gccgccgata
     6001 tattctttta accttccatt tccttcaaaa acaaggcttt gggtaatcac gttatcgagg
     6061 aacatacggt cgtgcgagac aaggaatacc gtgccttgat aatcgcgcaa caggtcttcg
     6121 agcagctctt gggtgtcgat gtccaagtca ttggtcggtt cgtccaagac caaaatattg
     6181 gcgggacggg taaagagttt tgccagcaaa aggcggttgc gttcgccgcc ggagagtgaa
     6241 gaaacaggac tttgcgcgcg ggcagggggg aacaggaaat cttccaaata gctcatcaca
     6301 tgctttttct taccgccgac ttcaacgtaa tcattgccct gtccgagcgt gtaaaacacg
     6361 gtgtcgtttt cattcaacgc gctgcggaac tggtcgaaat aggcgacttc ctgcttgctg
     6421 ccgatacgga ttctgccgta ggtcggctgc aattcgccca aaatcagctt gaggaaggtg
     6481 gttttgccga taccgtttgg gccgattaag ccgattttgt cgccacgctg caagatggcg
     6541 gagaatttgt ccataatgac tttgccgcca taggcaaacg aagcgtgttc caattcggcg
     6601 atgattttgc cgcttttctc accgctatcg agcttaaagt tgacctgtcc ctgcacgttg
     6661 cggcgttctg cacgttggcg gcgcagctct tccaaacggc gcacgcgtcc ttcgttacgg
     6721 gtacggcgcg cttcgatgcc tttgcgtatc cacgcttctt cctgagcatg aaatttgtca
     6781 aacaagcggt tatgttccgc ctcgactgcc aactcttgcg cttttttctc actgtattta
     6841 gagaacgagc cgggatagga acgtagaatg ccgcggtcaa gttcgacaat ccgcgtagcg
     6901 atattatcca aaaaacggcg gtcgtgggta atcacggcca agctgccttc aaacgccttg
     6961 agcaggtttt ccaaccagat aatcgcgtca atatccaaat ggttggtcgg ttcgtccagc
     7021 agcaatacgt cgggcttctg cacccaagcc tgcgccaagg cgacacgctt tttctgaccg
     7081 ccggaaaggt tgccgatttt ttcattttcc ggcaaactga gttcccccaa agtctgcttg
     7141 actgccgcat ccagcttcca gccatccttc gcttcgattt caagttgtaa ttcgttgagt
     7201 tctttcaaca aagcctcact cgaaccattt tccaactcat ggctgacatg atgataacgg
     7261 cgcaataaat cacgaatttc gcccaaacct tcggcaacgg tatcaaatac ggttgcgtcc
     7321 ttatcaaaaa aggattcctg cggtacataa acgattttga ggttgttttg aacaataatc
     7381 tgcccgtcgt cgagcttttg cacaccggcg aggattttta aaaacgaaga cttgcctgcg
     7441 ccgttgcgtc cgatcaagcc gactttttcg ccgctgtcga gttgaaaaga agttttgtcg
     7501 agcaaggcaa cgtggccgat ggcaaaagaa gcgttttcta cagataatat attcatgata
     7561 caaattctca acagttaccg tttggatttt accgcaagtt tggcgcgggc aatttcaact
     7621 gcacccggca ggacggagac aataatgatg ccgcccatca ccaagcccag attgtttttt
     7681 acgacgggga agttggcaaa gaaatagccc gcgtaagaaa acaggataac ccacaacaag
     7741 ccaccgatga tgttgtagcg gataaatttg gcatagtgca ttttccccat accggcgacg
     7801 aagggggcga aggtgcggac gatgggcata aaacgggcaa tgatgatggt tttgccgccg
     7861 tgtttttcgt aaaaacggtg ggttttatcg agatattcac gtcggaagat tttagaatcg
     7921 gggttggcga acagcctgcc gccgaaatat ttgccgacgg taaaattgag cgcgtcgccg
     7981 agtatggcgg caaggcttaa taatgcaacc atcaaatgaa tatccatacc gcccagcgcg
     8041 gcaatcccgc cggcggcaaa cagcagcgaa tcgccgggca gtaagggcgt aacaatcagg
     8101 ccggtttcgc aaaaaacaat caaaaacaga atcgcataaa tccacacacc gtattgcgcc
     8161 gacagcgcga gcaggtgttg gtcgatatgg aggatgaagt cgatggcgga ggcaagcacg
     8221 gcgcgttcct aaaaaaaaca aaccgcgtat tttaaccgat tggaaaaatg ccgtctgaaa
     8281 agtttcagac ggcatcggct attcaaattc atttcacgta aaaaccgcaa accaaaatag
     8341 tttgcggttt ggcatttaaa gtgacaatga tgatttcaaa tcatcagaat tttatgccga
     8401 cgcgcaagcc gtattcacga atactggttt tcgggatggt gagcgatacg tcgccactct
     8461 tggttgttac actaaactcg ccggattctt tgtaagtgcg ttgtttgtag aacggccccg
     8521 cctcgatgct ggcggattcg cccagttttt taccaatatt tgcacccaat ccaaagcccc
     8581 agccgttggt tttttgattg atgtcttttt tgagactggt aacgccggtg tttaatttat
     8641 agcgggaatt gaggtcaaat ttcacttcag accaagggtt gatataccag ccgttaccca
     8701 gttgggaaag caaatccgcg tgaactttgg ctgaccacga ctgacggctg ctgtgaagcg
     8761 tatgcttggt ggttttaatg ctgtcttttg aagattcaaa acccaagccg gcacccacac
     8821 ggaaatttaa agaatcactt aacgtttggg tataggtgta gcctgtgtaa agatcgatac
     8881 ggttttcagg aacgccggtg ggcagtttta catttttagt cttacccagc ttgttctcat
     8941 ctgtttccaa attaataata ttttttttgc tgcgcccgaa accggcttcc aagcggatgc
     9001 cttggctggc atcaaaagga atattagcac gtacgccgat gtgtttggca gctttgtgtt
     9061 tttctttcag gaaagcacga gttgaagaaa tggaagagag gtcggtgtgg acggtaaact
     9121 cattagcggt ttgaagctct tgtgcagcgg cggcagtacc ggtcagggcg atcatggcac
     9181 atgtaaaaac tgtttttttc atagttaaaa cctctaaaat ttggattgta gtcggatatg
     9241 gtaacataac gtaaataatc gttacgctta caattatatt cttaagcttt cggggggggg
     9301 gggattttac atatattaat aaaaattaac aaatagttat ttgtttacaa cgaattgtta
     9361 ttctcacttg gttttctgtt ttttatggga atgacgaaat tttagtttgt gtgtatttat
     9421 cggaaaaaca gaaacccgcc gccgtcattc ccgcgaaagc gggaatctag aacccaacac
     9481 ggcaaaaatt tatccgaagc gacaacaat
//
LOCUS       DAWFJW010000073         9317 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFJW010000073 DAWFJW010000000
VERSION     DAWFJW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.073"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..201)
                     /locus_tag="WGR19_001439"
     CDS             complement(<1..201)
                     /locus_tag="WGR19_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0798949.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMP"
     gene            440..1339
                     /gene="crgA"
                     /locus_tag="WGR19_001440"
     CDS             440..1339
                     /gene="crgA"
                     /locus_tag="WGR19_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0798950.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1565..4780)
                     /gene="carB"
                     /locus_tag="WGR19_001441"
     CDS             complement(1565..4780)
                     /gene="carB"
                     /locus_tag="WGR19_001441"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0798951.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLVKEEKSVSDGILSDLDFAALRRLKRKGFSDKRIAQLLGVKEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4809..5450)
                     /locus_tag="WGR19_001442"
     CDS             complement(4809..5450)
                     /locus_tag="WGR19_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798952.1"
                     /translation="MSIPINFNNLKYLFNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLKFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(6393..6782)
                     /locus_tag="WGR19_001443"
     CDS             complement(6393..6782)
                     /locus_tag="WGR19_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0798953.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7204..7581)
                     /locus_tag="WGR19_001444"
     CDS             complement(7204..7581)
                     /locus_tag="WGR19_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEZ0798954.1"
                     /translation="MKISALDHLILTVADIDRTIAFYTQVLGMEEVSFGNNRKALLFG
                     SQKINLHARGAEIQPNAQHAACGTADLCLLTDTPLETVLQELSAHGIKPLSGIAARTG
                     AMGKIQSVYLRDPDGNLLEISSY"
     gene            complement(7627..8760)
                     /gene="carA"
                     /locus_tag="WGR19_001445"
     CDS             complement(7627..8760)
                     /gene="carA"
                     /locus_tag="WGR19_001445"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0798955.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYASGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2074 a         2382 c         2449 g         2412 t
ORIGIN      
        1 cggcatctgc caaatcacag catccatcca aacgaatttt tcgatttctg cctcaacatc
       61 atagccggca tcaatcacgg tttcttgaac attgtgtccg agcgcggtca aaacttcttt
      121 cgcttttttg tgaagcgtgc ggtttaaccc gccgtgagaa tgtccgaacg ccttgccgcc
      181 gtctaataat aaaatattca tttgttacct cgtttgtgaa ttgatgaatg tattgtgccg
      241 ttttacttta taatttaaaa gtgcaaaaat aagaaaacac ttttgcgtca aatgaaataa
      301 tcagatgttc caacacacgg gatggcacat aaagcaccgc cctgtgtgcc gtcctgattc
      361 ggaaggggtt acacccctca caaataaagt ctgatcctgc cgccctaaag ggcggggttc
      421 caaccgaaaa ggaaacacga tgaaaaccaa ttcagaagaa ctgaccgtat ttgttcaagt
      481 ggtggaaagc ggcagcttca gccgtgcggc ggagcagttg gcgatggcaa attctgccgt
      541 aagccgcatc gtcaaacggc tggaggaaaa gttgggtgtg aacctgctta accgcaccac
      601 gcggcaactc agtctgacgg aagaaggcgc gcaatatttc cgccgcgcgc agagaatcct
      661 gcaagaaatg gcagcggcgg aaaccgaaat gctggcagtg cacgaaatac cgcaaggcgt
      721 gttgcgcgtg gattccgcga tgccgatggt gctgcatctg ctggcgccgc tggcaacaaa
      781 attcaacgaa cgctatccgc atatccgact ttcgctcgtt tcttccgaag gctatatcaa
      841 tctgattgaa cgcaaagtcg atattgcctt acgggccgga gaattggacg attccgggct
      901 gcgtgcacgc catctgtttg acagccgctt ccgcgtaatc gccagtcctg aatacctggc
      961 aaaacacggc acgccgcaat ctacagaaga gcttgccggt caccaatgtt taggcttcac
     1021 cgaacccggt tctctaaata catgggcggt tttagatgcg cagggaaatc cctataagat
     1081 ttcaccgcac tttaccgcca gcagcggtga aatcttacgc tcgttgtgcc tttcaggttg
     1141 cggtattgct tgcttatcag attttttggt tgacaacgac atcgctgaag gaaagttaat
     1201 tcccctgctc gccgaacaaa cctccaataa aacgcacccc tttaatgctg tttattacag
     1261 cgataaagcc gtcaacctcc gcttgcgcgt atttttggat tttttagtgg aggaactggg
     1321 aaacaatctc tgtggataat tctataaaaa cctgccataa aattgttttt actcacttaa
     1381 taactttgag aaaaaataaa taagatgatg tatagccgcg gcaatggcag tctatatggg
     1441 gagcgtgata gggctgttcc ttatgtacgg cttgtgtgtg gcaacatcgg ctttccaaat
     1501 gacaaaatgc cgtctgacac cgtaaaaaaa ggtttcagac ggcattttca acctgattca
     1561 ggtatcagtt gcggtttttc aaacgcccgt gcaactcttg aacgctgtac acgcccagat
     1621 ggtcgcggct tttcgcgcct tcgctcatcg cttcgccgcc ggcagtggtg gtgtattgcg
     1681 gcacacgttg ctgcaatgcg ctttggcgga tgatgtggct gtcggacacg gattgcggat
     1741 cgctggaaac ggtattcacg accagtgcga tttcgccgtt tttcagcgcg tcgccgatgt
     1801 gcgggcggcc ttcggggact ttgttgatgg tctgcacaat tagcccgtgt tcggtcaggt
     1861 attgcgccgt gccgcgcgtg gcgcagatgc cgtagcctaa ggcttggaag tttttggcgg
     1921 ttttaatgac gcgttctttg tcttcttcgc gcacggagag gaagattttg ccggtcgggt
     1981 tgaggcgttc gcccgcgccg agttgggctt tgtagtaggc ttcgccgaag cttgcgccca
     2041 cgcccatcac ttcgccggtg gagcgcattt ccggtcccaa aatcgtatcc acgcccggga
     2101 atttgatgaa tgggaacacg gcctctttaa cggcatagaa atcggggacg acttcttttt
     2161 ccacgccttg ttctttcagg gaaatgcctg ccatgcagcg cgcgccgact ttggcgagcg
     2221 gcacaccggt ggctttggag acgaaaggca cggtgcggct ggcgcgcggg ttcacttcca
     2281 atacgaacac cacgccgtct tgtacggcaa actgcacgtt catcagtccg accacgccca
     2341 gcgcgtacgc catcgctttg gtttggcggc ggatttcgtc ttggatttct tcgcttaagg
     2401 agtagggcgg cagcgagcag ccggagtcgc cggagtggat gcccgcctgt tcgacgtgct
     2461 gcataatgcc gccgataacc acgtctttgc cgtctgaaac gcagtccaca tccacttcaa
     2521 tcgcgttgtt caggaagaaa tcaagcagta cggggctgtc ttcggaaacc tgcacggctt
     2581 cgcgcatgta tttttgcaag gcttcggcag agtggacaat ctgcatcgcg cgtccgccca
     2641 aaacataaga cgggcgcacg accagcggat agccgatttc ttcggctttg acgagtgctt
     2701 cttcttcgtt gtgggcgatg cggttgggcg gttggcgcag acctaagtcg ttcaacactt
     2761 tttggaagcg ttcgcggtct tcggcggcgt cgatgctgtc ggcggacgtg ccgatgatgt
     2821 ttacgccgtt ttcaaccagc gcgttggcga gtttgagcgg ggtttgaccg ccgtaatgca
     2881 caatcacgcc ccacgggttt tcggtgcgga cgatttccaa cacgtcttcc aatgtcagcg
     2941 gctcgaaata gaggcggtcg ctggtgtcga agtcggtgga cacggtttct gggttgcagt
     3001 tgaccataat cgtttcaaaa ccggattcgc gcagggcgag cgcggcgtga acgcagcagt
     3061 agtcaaactc gatgccctga ccgatgcggt tcgggccgcc gccgagaatc atcacttttt
     3121 tacggtcgga aggacgggat tcgcattctt cttcgtaagt ggagtaaaga taggcggttt
     3181 cggtggcgaa ctcggcggcg caggtgtcaa cgcgtttgta aaccggatgc agcttcagcg
     3241 cgtagcggtg ttcgcgcact tctttttctt ttacgcccaa aagctgcgcg atgcgtttgt
     3301 cggagaagcc tttgcgtttc agacggcgta gggcggcgaa atccaaatca ctcaaaatgc
     3361 cgtctgaaac cgatttttct tctttcacca agtcttcgat ttgcgccaag aaccaagggt
     3421 cgatggcgca gatttcgtgg atttcttccg gcgtgaagcc cgcgcggaac gcgtctgcca
     3481 caaacagcat gcgctcgggg ccggggtttg ccagttcgcg gcggatttcc gctttgtcgg
     3541 agcttctcgg attgaagccg cacaagcctg tttccaagcc gcgcagggct ttttggaaac
     3601 tttcctgaat cgtgcgtccc atcgccatca cttcgcccac cgatttcatc tgcgtggtca
     3661 ggcggtcgtc tgcggcaggg aatttttcaa acgcaaaacg cgggattttg gttaccacat
     3721 agtcgatgga cggctcgaat gacgcggggg ttttgccgcc ggtgatgtcg ttgcgcaact
     3781 cgtccagctt aaagccgacc gccagtttcg ccgccacctt cgcaatcggg aagcccgtcg
     3841 ctttggaagc cagcgcggat gaacggctca cgcgcgggtt catctcaatc acaatcatct
     3901 cgccgttttc agggttcacc gcaaactgca cgtttgagcc acccgtgtcc acgccgattt
     3961 cgcgcaatac tgccaacgaa gcattacgca tgatttggta ttctttgtcc gtcagcgttt
     4021 gcgcaggcgc aaccgtaatc gagtcgcccg tatgcacgcc catcgggtcg aagttttcaa
     4081 tcgagcagat aatgatgcag ttgtcgttct tgtcgcgcac cacttccatc tcgtactctt
     4141 tccagccgag aacagactgc tcaatcagca gctcgtgcgt aggcgacgca tcgaaaccgc
     4201 gttcgcaaat cgccaaaaac tcatctttat tgtaggcaat gccgccgccc gaaccgccca
     4261 tcgtgaaaga cggacgaatc agggtaggga agccgacctg ttcttgcgct gccaaagctt
     4321 cgttcatcgt gtggcagaca aaagatttcg ggcaggagag gccgattttt tccatcgctt
     4381 ctttaaaacg accgcggtct tccgccttgt caatcgcgtc ttccgtcgcg ccgatcagct
     4441 cgacattgta tttcgccagc acgccgttgc gcgccaaatc cagcgcacag ttcagcgcgg
     4501 tctgaccgcc catcgtgggc agaatcgcat caggacgctc cttggcgata atcttctcca
     4561 ccgtctgcca cataattggc tcgatgtagg taacatccgc catttcaggg tcggtcatga
     4621 tggtggcggg gttggaattc accagaatga ctttatagcc ttcttcacgc aaagccttac
     4681 aggcctgtgc gcccgaatag tcaaattcgc aggcctgacc gataacgata gggccggcgc
     4741 cgatgataag gatggatttt aggtcggtac gtttgggcat ggtgggtctt tcttttgaag
     4801 aatttggatt attgacgatt ggtaacttct ctaattattt ctgcatctgt tttttcatcg
     4861 cttggattat ctgaaaagaa aaaactgtaa ttactatcgg ctttaaattt ttcgtatagc
     4921 tctgggcgca aaatttttgc tttttcacta ttttccacgc tcctgcgatt aggcttgcca
     4981 gccttatctt tcccatttct acggacatca tagcaataaa tagtgttagg agaattaggt
     5041 tctaaacgag tagctacaat ctttttataa attatgtcta aatctttttt agtttgtgtt
     5101 ggaatgaaat ctgcaaaaag attactaaaa ctctgaaaaa tttctctgat tttcgcagca
     5161 ccagcctgaa cattaatttt aaaaaaatta ataaaatcag tagcactttt aaaatgaact
     5221 tcataaaaat cagcatacgc aaatattgaa ttttcactat tcaaattaaa aaactttaat
     5281 tttgcttgtg cataatcatc tggtctaggt tcatcaggtt tatacctagt caaaataacg
     5341 gcgtattgcc tttgattata attaaaagga aatgcttcaa ttattctatt tttgtttctc
     5401 atatcattaa acaaatactt taagttattg aaattaatag gtattgacat atttaaactc
     5461 ctgcgaatat gttgcaataa acgtagcaat cgtaggttgg gttgaaaacc caacacaaac
     5521 cgcttaattg tctaaaatgt tgggtttgct aacctaactt gtatatcgaa acccccgtat
     5581 tgtagcaagc cgtagcccgc atgtagggta tgtgcggtac gtacgcacgc gttctccatt
     5641 ttccctgcaa ccccaaccca ccgtgtgcgt gccttgcggc acactctctg ccgacggatt
     5701 caaaggtcgt ctgaaaaaac gctcgcggca tttgttccat cttccttgcg ataaaggcta
     5761 cctgaaaaat tcctgctgtt gatatctcgg gcattctttc tcaaaccgcc tgcaactgcc
     5821 aaatctgctg caacctgccc cctcccccgt gggggagggc tggggagagg gcattctccg
     5881 aatttcggca acctttccca acaactcaac caccgaaata caagccttgt ggcttgttgc
     5941 cctctctcta gctctctccc acgggagaga ggacggagtg gctgtagggc taagggttct
     6001 gcaaggaaac aggttgtgca ggttgctttt tgcttttcag acgacctgaa atcaacggta
     6061 aatccaagtc gcttttcaaa ccgcctgtaa ccgcaaaact tgctgcaacc taccccctcc
     6121 cccgtggggg agggctgggg agagggcatt ctccgaattt cggcaacctt tcctaacact
     6181 ctaaccgcca agacacaagc cttgtggctt gttgccctct ctctaactct ctcccacggg
     6241 agagaggact gggcggttgt tggggctaag gtttctgtaa actaatcagg ttatgcaggc
     6301 tgctttttgt ttttcagacg gcctttataa atgctcaatt cagtcggctt tatcctatta
     6361 ttttcaataa tccaatcaaa atcagccatc tgctatcgtg cagcttgctt ttccaattct
     6421 tgcaacacga gcaggatttc tgtcagcaca tcgtttgtct gctgtaaaat ctcgtgattc
     6481 caaaaccgca gtacggtaaa gcccaagctg ttgagatatg ccgtccgcgc gtggtcgtat
     6541 gcggcttgtt ctgtgtgctg ccctccgtct gcttcgacaa tcaacttggg cgttacgcac
     6601 ataaaatcaa caatatagtt tcccatcggc tgctggcggc ggaatttata gccgttcaga
     6661 cgacctgccc gcaggtgctg ccacaatttt gcttccgccc cgctcatttc ttggcgcatg
     6721 gctttggcgc gttggcgcaa gatggggttg tcggtggtca agagtttctc gtgcgggttc
     6781 attttgttgt cgtgctgttt gctgccctct ctctaactct ctcccacggg gagagaggac
     6841 ggggcggttg ttggggttaa aggggctgta aggaaaacaa gtcgttcagg ccacttttta
     6901 cttttcagac gacctgcaat caacggttgt aaatccaagt tgcttttcaa accgcctgta
     6961 accgcaaaac ttgctgcaac ctaccccctc ccccgtgggg gagggttggg gagagggcat
     7021 tcctcgaatt tcggcaatct tcccaaatac cctaaccgcc ccaatacaag cctttcggct
     7081 tactgccctc tctctagctc tctcccacag ggagagagga tggggcggct gttggggtta
     7141 aagaggctgc aaggaaaaca gcttgtgcag gctgcttttt attttgcata agccgtctga
     7201 aaatcaataa ctgctgattt ccagcaggtt gccatcggga tcgcgcaggt aaaccgattg
     7261 gattttgccc attgcgcctg tgcgcgctgc gatgccgctt aaaggtttga tgccgtgtgc
     7321 ggataattcc tgtaaaaccg tttccagtgg cgtatcggtc agcaggcata aatccgctgt
     7381 gccgcaggcg gcgtgttgcg cgttaggctg aatttccgca ccgcgcgcgt gtaggttgat
     7441 tttctgactg ccaaacaaca aagccttacg gttgttgcca aatgaaactt cttccatacc
     7501 caaaacttgc gtgtaaaacg caatggttcg gtcaatgtcg gcaacggtca gtatcagatg
     7561 gtcgagtgcg ctaatcttca tttcccgctt tctttttcag gcggcggcag tgccgtctga
     7621 aagccattat gcccgttttg ccgctttcat attgccaatg aatttgtcaa acaaatagcc
     7681 gacatcttgc ggaccggggc tggcttcggg gtggccttgg aagcagaaca caggtttgtc
     7741 ggtcagctcg atgccttgca aagtgttgtc aaacaaggat ttgtgggtaa tgcgtgcgtt
     7801 agcgggcagg gtgtcggcat caacggcaaa accgtggttt tggctggtga tgacgacttt
     7861 gccgctgtcc aaatcttgca cagggtggtt cgcaccgtgg tggctgaagc gcattttcag
     7921 ggttttcgcg ccgatagcga ggctgatgag ctggtgtccc aagcagatgc cgaaaatcgg
     7981 tttgccgctt tccatcagtt tttgcacggc ttcgatggcg taggtgcaag gctcggggtc
     8041 gccggggccg ttggacagga acacgccgtc gggattgagt gccaacacgt cttctgcgct
     8101 cgtttgggcg gggacgacgg tcaggcggca gccgcgcgag gcgagcatac gcaggatgtt
     8161 ggttttcacg ccgaaatcgt aggcgacgac gtggtaaggc tgttcgtcag gggtaacgaa
     8221 acccttgccc aattcccatt cgccttccgt ccattcgtaa gtttccgtgc aggaaacttc
     8281 ttttgccaag tctttgccga ccatactgcc gaacgcggcg atgagttctt gcgctttttc
     8341 gattgtggca tccgcaccgg tcagaatcgc gccgccttgc gcgccttttt cgcgcaacag
     8401 cgtggtcagg cggcgggtat cgatgtcggc gatggcgacg gttttgttgc gtaccaaata
     8461 gtcgtgcagg ctttcggagg cgcggaagtt gctgtgcaag agcggcaggt cgcggataat
     8521 caggccggag gcataaacgc tgcggctttc ttcatcttcg gcgttggtgc cggtgttgcc
     8581 gatgtgtggg taggtgaggg taacgatttg tttgcagtag gacgggtcgg tcaggatttc
     8641 ctgatagccg gtcatcgaag tattgaacac gacttcgccg gaagtcgaac cttcgtaacc
     8701 gattgatgtg ccgtgaaata cgctgccgtc agcgaggacg aggagggcgg gggtgctcat
     8761 gatgggaatc ctgtttttaa taaaattctt gacaatgccg tctgaaggga cttcggcagg
     8821 ggcatccggt cggaaaaatc cggtcaaaaa aaaacacgcc gcgcaagata gacgcgtaac
     8881 gtgcttttcg ggcaagtgcc tataccctga aagcagcata ttttaagacg aaacataccc
     8941 gatttcaagc gcaaacgcgg caggcgggct gatatgttcg cgcgaaaacg gaaatccgtg
     9001 gcgtaaaacc gcccgctctg cgcccactat gcccgttacg catttcccca cgtccgtccg
     9061 gcgaaactat gggaataccc gaatcgtcag gaatacccga accatcattc ccgcgcaggc
     9121 gggaatccag acctgtcggc acagaaactt atcgggcaaa acggtttctt gagattccgc
     9181 atcctagatt cccgcctgcg cgggaatgac ggcgcataag tttctgtgtt gatttgagtc
     9241 tatgggaacg gtgtaggatt ggtggaatcg ttggaatcgg tgggctgaag cccaccctat
     9301 aactcacctt ttacgaa
//
LOCUS       DAWFJW010000074         8973 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFJW010000074 DAWFJW010000000
VERSION     DAWFJW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.074"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(195..407)
                     /locus_tag="WGR19_001446"
     CDS             complement(195..407)
                     /locus_tag="WGR19_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0798956.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(419..700)
                     /locus_tag="WGR19_001447"
     CDS             complement(419..700)
                     /locus_tag="WGR19_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798957.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(747..1010)
                     /locus_tag="WGR19_001448"
     CDS             complement(747..1010)
                     /locus_tag="WGR19_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798958.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1030..1603)
                     /locus_tag="WGR19_001449"
                     /pseudo
     CDS             complement(1030..1603)
                     /locus_tag="WGR19_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1582..1893
                     /locus_tag="WGR19_001450"
     CDS             1582..1893
                     /locus_tag="WGR19_001450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798959.1"
                     /translation="MTSPVSPSIRAASIFPLVRSISTACRTDINRLPLRLIASTKTAE
                     INLASSVLSSVLPASLRRLRVGLMLTASSLRYSVARLMPNSCACCLRYAERAPRPIAS
                     A"
     gene            complement(1915..2790)
                     /locus_tag="WGR19_001451"
     CDS             complement(1915..2790)
                     /locus_tag="WGR19_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0798960.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2835..3125)
                     /locus_tag="WGR19_001452"
     CDS             complement(2835..3125)
                     /locus_tag="WGR19_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0798961.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3137..3313)
                     /locus_tag="WGR19_001453"
     CDS             complement(3137..3313)
                     /locus_tag="WGR19_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0798962.1"
                     /translation="MAITVEKLKENFAKNGQTLAQWARENGFKPRDVYLVVGGQRKGN
                     YGKGHEIAKKLGLK"
     gene            3431..3802
                     /locus_tag="WGR19_001454"
     CDS             3431..3802
                     /locus_tag="WGR19_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0798963.1"
                     /translation="MLFVGFLERIKQELDLKKDKDVAEFLGLEPKAFSIRKKRDSIPV
                     DAVRAAAFEHPELNADWGYILTGRREKSVKMPSEKLNKEEQELLALFREAAAADREMI
                     LMVARRAEKKAQAALGKVSNG"
     gene            3807..4109
                     /locus_tag="WGR19_001455"
     CDS             3807..4109
                     /locus_tag="WGR19_001455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798964.1"
                     /translation="MNTVLTPKLIEKLERLRSLEIEAQELRAELQPWLDNACKQYFVA
                     WLENQLEKGRDISVLELKSNLSDLAYHAVMNDNIALWHQVSEVKKLLAIYEAKRKT"
     gene            4113..4283
                     /locus_tag="WGR19_001456"
     CDS             4113..4283
                     /locus_tag="WGR19_001456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798965.1"
                     /translation="MPSEIFRRHLGHSDSWARCSETDIKQLKRPCILRRIKNHPDIQV
                     IGQVVQVSKNLY"
     gene            4328..4534
                     /locus_tag="WGR19_001457"
     CDS             4328..4534
                     /locus_tag="WGR19_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEZ0798966.1"
                     /translation="MMFASPTPALAKSCKDFPTQQAAQKFYEARKKAGLSGWKSLDRD
                     GDGWACDCNPGGSGKNCPKKKDNF"
     gene            4545..5132
                     /locus_tag="WGR19_001458"
     CDS             4545..5132
                     /locus_tag="WGR19_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798967.1"
                     /translation="MKTKIYLGIFLLAAAHLVNAGGVMKTGGMIGPHPETFSEQFNEL
                     NNGMMDLFPRISIVAGKTRQTKDLGNGITLSAKLVKEGDIFENVRINCNTLAKTQRIN
                     NCLVSMYYTAIALDYEIDRLAFMNAIQTAIKRMHATYNQNGVDYFITVNRKQKSVSMF
                     AKSDMPMQIEAMDDSNEAYMKSDKCNPEIELCIKP"
     gene            5226..5729
                     /locus_tag="WGR19_001459"
     CDS             5226..5729
                     /locus_tag="WGR19_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0798968.1"
                     /translation="MSDKFNQFINRVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            5743..5916
                     /locus_tag="WGR19_001460"
     CDS             5743..5916
                     /locus_tag="WGR19_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798969.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRRK"
     gene            5841..6179
                     /locus_tag="WGR19_001461"
     CDS             5841..6179
                     /locus_tag="WGR19_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798970.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWTNVAAASMTYKFSQTADAPEWLWWAYGALVGGYALIKRGIAAVPQLAEIKKS
                     ANAEEWSGND"
     gene            6172..6582
                     /locus_tag="WGR19_001462"
     CDS             6172..6582
                     /locus_tag="WGR19_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0798971.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACFTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            6587..7186
                     /locus_tag="WGR19_001463"
     CDS             6587..7186
                     /locus_tag="WGR19_001463"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0798972.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKQHIHDIYRR
                     HKGRYGYRRIAAAILHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLTKIRTRR"
     gene            7183..7335
                     /locus_tag="WGR19_001464"
     CDS             7183..7335
                     /locus_tag="WGR19_001464"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0798973.1"
                     /translation="MKPQTVQIVRQGEATPYWFKFNPLYNNDRIKLKLKGLSLVQYRI
                     QSLKAA"
     gene            complement(7654..8016)
                     /gene="ssrA"
                     /locus_tag="WGR19_001465"
     tmRNA           complement(7654..8016)
                     /gene="ssrA"
                     /locus_tag="WGR19_001465"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8079..8684)
                     /locus_tag="WGR19_001466"
     CDS             complement(8079..8684)
                     /locus_tag="WGR19_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0798974.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2128 a         2083 c         2373 g         2389 t
ORIGIN      
        1 gaaaacagct gaaaacctgt gtttgggttt cggctgtcgg gagggaaagg aattttgcaa
       61 aggtctcaga gtgagtttat tttggggcgg cggcaggtcg ggggcaagcg gcgtggggct
      121 tggttgtggt ttttaggttt gggggtgggt aaaaaatgcc gtacccggcg ggcaggcttc
      181 gcactctgaa ggtttcagac ggcgtttgtt ttttctgtcc aatcgaggaa ctgccgccat
      241 ttttccagcg gcatatcggc ccgccgcgtt tcggtatcgg gttcggcttc ccagcggcct
      301 acctgtatgt agtgcttcac cccgacgatt tgcgccaact cggcctgtgt cagtttgcag
      361 cggttgcgca gggtgcgaag gttgtaaggc gtgtagccga gttccatgtc gttgcggttt
      421 attttgtttc gcatattttt ttgactgccc ggcggcaggt ttcggtaagg acggcggcaa
      481 atcgggcttc gtctgccggt tttccgtcga aaaaaataaa atcgtaaagg gtgatgccgt
      541 tttcggaatg gcggtaatgc ccgcccgggc attcgctgtc tgtctgcctg tggattttgg
      601 caatcaggcg gggatagcgg caatgggtaa tgaaacggct tgcgccgtct tgcccgataa
      661 tccattcggg gtagcggttg aatagtgcgg ctctgttcat tttgttcggg ttgttcgggg
      721 gataaagccc ctcgcggggc ttgtggtcag gcaaatttga atatcagtgc caacacggcg
      781 gcgatggcgg taaccagccc ggtggccgcc atcatgggat accatcggga ttcttgggct
      841 atttttacgg attcggcgtt gattttgtgc gcgtctgcaa tgattttggc gatttcggca
      901 tctacttttc tcagactgga gtgtttcatt tcttgttcga ttatgttcat cgtacttcct
      961 ttcgtttttg gcggttgccg ccgcttgtcg gatggtagga tgtctgccat gtgtatatat
     1021 tgataccttt taggttttat tgcaagtgtt ttgggcggcg gcttcgtatg cttggcggtg
     1081 gcggcggctg taccattcgg cgagttcgcg ccgctcttgg aggcggtagc tgtcggcggt
     1141 caggtagtgg tgcaggcttg agaagccggc gcgttggagg gcggcgcggc tgatgtggcc
     1201 gagggcgagg tctgttgtga gggtgtcttt tccggcggtc aggctgatgt tggtgaagag
     1261 gtggcggaat ccgtgcattg tgtgtttgga tttgccgggg gtgctgccgt catagcccag
     1321 tcgtcggatg gcgttgtggg cgaatttgat gctgatgtgg tcgggatggg ggggcgggtt
     1381 tgcgccgtgg gcggatgccg gggaaaaggt ggatgttgtc gccggtctgc gtgtgcagct
     1441 ctcggagtat ttctaccgcc cagtccgaca gtgggacggt aaaggggtgt ttggtcttca
     1501 tgtcggcggc ggggatgtgc cataaccggg cggtgaggtc gatgtcttgc cagcgggcgg
     1561 aaagcagtgc ggacggacgg gatgacttcc ccggtttccc catcaatccg cgccgcctcg
     1621 atttttccgt tggtgcgcag catttccacc gcctgccgca ccgacatcaa ccgcttgccg
     1681 ttgcgcctca tcgcctccac caaaaccgcc gaaatcaatt tggcttcttc cgttttaagc
     1741 tccgtcttgc ccgcatcgct gcgccgtttg cgcgtcggct tgatgctgac cgcctccagc
     1801 ttgcggtata gcgtggcaag gctgatgccc aattcctgcg cctgctgctt aagatatgca
     1861 gagcgtgcgc cgcgtcccat tgcttccgcc tgattctcga ctgccttaag acgctcaatc
     1921 attgccggat tcatcgcctt ctcccgtttc accgcccaac cattccggca cattgtctgt
     1981 cggtgcttca gtcggcaggg catagctttc gcgcagttgc tcgcagtcca aaataatttg
     2041 attgagcgtg ccgaccatct ttgcctgatg gctgatctcg tgtgcctcac tgtgcgcatt
     2101 aagttggtcg aacaaatctt tcaaacggct cacttgactg cggataccga cctcaaggct
     2161 tgttaactgc atcgccaact cactgcccac gtcttccgcc ttcggctctc tgacaacggt
     2221 ttgcttctta gccagcttct cggtcagctc atcaattttg gctgttttgg ttttcatcac
     2281 ttcgtctttg gcggcgaggt tttcacggct ttcgcgcagg gcgacgcgca gctcgcgcac
     2341 cgtcattcgg tccacatcgt caaaggtcat gccgttgact tcttctcctt cggcaagtcc
     2401 caccaacgta acgtcttctt cgaccaagag ttccagcagc tttgacttgc ccaaatccat
     2461 cagcttcggc gcggctttct gcatttgcgg ggtcgcaaag cggcgagtgg ctgacatcag
     2521 acgtgatgtt tctgcgatac ccagcccaaa ttggcttttc acaatttcca taaaccgtcc
     2581 gtgttccgta tgctctttta aaataatcag cgcacgtccc agttcgaaca tgccttccat
     2641 cgtctgccgt actgcttgac gaccgcgttc aacccaacgc tcttcgctat aagtctcgcc
     2701 gttaccccac tgctccatca ccagcacact gcgcatcgct gcctgattgc ttactttgtc
     2761 ggttgcgata atttcaattt ctgtattcat tttttatctc caaagtttcc gacgtcggaa
     2821 actttcaaaa tccgttaatc cacatcgacc cgcttgccga tttcggcaat cttgctttgc
     2881 agccgttcat gctgctgcct gaaccgctct gcgatttgca gggttttgat gccgtaggcg
     2941 tagttgccgt tttcaagttt gatgaccaat cccgaggcaa ccaaatcatc aatatccctg
     3001 ctgacttgcg atggcgtcag ccccagtccg accgataaat ccttattgct cagaccgata
     3061 atcggatgct cgtcaagcgc gataaagacc ctcaatagcc gttgtaccct tttactttct
     3121 gccatccgca tcctccttat ttcagtccca gcttcttggc aatttcatgc cccttgccgt
     3181 aattgccttt gcgctgtccg ccgaccacca gatacacatc gcgcggctta aagccgttct
     3241 ctcttgccca ctgcgccagc gtctggccgt ttttggcaaa attttctttt agtttttcaa
     3301 cagttatagc catagatagc cctttcccat tgtgataaaa tagtgaacta tttatgcacc
     3361 agatatttac tagtgcgctc cataaacaga atattaatct acttttgtag attatttcaa
     3421 gagattttgt atgctttttg tgggtttttt agaaagaatt aagcaagagc ttgatttaaa
     3481 aaaggataag gatgtcgctg aatttttagg actagagccg aaagcgttct caattcgaaa
     3541 aaaacgcgac tcgataccag tagatgcagt acgcgcagca gcatttgaac atccggaatt
     3601 aaatgctgat tggggatata tcttgactgg acggcgagaa aaatcagtaa aaatgccatc
     3661 tgaaaaatta aacaaagaag agcaggagct gctggccttg ttccgcgaag cggcagctgc
     3721 cgaccgtgaa atgattctga tggttgcgcg cagggcagag aaaaaagccc aagctgcgct
     3781 tggtaaagtg agcaatggat aatgatatga atacggtgtt gacgcccaaa ttaatagaaa
     3841 aattagaacg attaagaagc cttgaaattg aggcacaaga gttgagagcg gagctgcaac
     3901 cttggttaga taatgcttgc aaacaatatt ttgttgcatg gcttgagaat caactggaaa
     3961 aaggtcggga tatttctgtt ttggagctaa aaagcaattt gtcagattta gcttaccatg
     4021 cggtgatgaa tgacaatatc gcattgtggc atcaggtaag tgaagtgaaa aaattgcttg
     4081 ctatttatga agctaagcgg aaaacataaa aaatgccgtc tgaaattttc agacggcatt
     4141 taggacactc cgacagttgg gctcggtgtt ctgaaacgga tattaagcaa ttgaagcggc
     4201 cgtgcatttt aaggcgcatt aaaaaccacc ccgatattca ggtcatcggg caggtggttc
     4261 aggtctcgaa aaacctgtat taaagagaca aaatgaaaag ggttatttga cattgcttgt
     4321 gtctgttatg atgtttgctt cacccacgcc cgccttggcg aaaagctgca aagactttcc
     4381 gacgcagcag gcggcgcaga aattttatga agcccgcaaa aaagcaggat taagcggctg
     4441 gaagagtttg gacagggacg gcgacggatg ggcttgtgac tgcaacccgg gcggaagcgg
     4501 caagaattgt ccgaaaaaaa aagataattt ctgaaagaaa tagaatgaaa acaaaaatat
     4561 atcttggaat tttcttattg gctgctgcac atctggtcaa tgcgggagga gtgatgaaaa
     4621 caggagggat gattggtcct catcctgaaa cattttcgga acagttcaat gaattgaaca
     4681 atggaatgat ggatttgttc ccaagaatat caattgttgc gggcaagaca cgtcaaacaa
     4741 aagaccttgg taatggcatt acgttgtctg caaaactggt caaggaagga gatatttttg
     4801 agaacgtccg gataaattgt aatacattgg cgaaaactca aagaatcaac aattgcttag
     4861 tgagtatgta ttacacggct attgcgttgg attatgagat agaccgtttg gcatttatga
     4921 atgctattca gactgctatc aaaaggatgc atgctactta taatcaaaat ggggtagatt
     4981 atttcattac ggtcaatcgt aagcagaaaa gtgtttctat gttcgcaaaa tcggatatgc
     5041 ctatgcagat tgaggcaatg gacgattcaa atgaagcata tatgaaaagc gataaatgca
     5101 atcccgaaat cgagctgtgt attaaacctt agcctcagga aggttttaac ctccattaaa
     5161 agcccgttca gacggccttt cctacaatcc ccgtattgat ttcaacctca atacggggat
     5221 tttccatgtc agacaaattc aaccaattca tcaaccgcgt cctctcccac gagggcggtt
     5281 acgtcaacca tcccaaagac cccggcggcg aaaccaattg gggcatcact aagcgcaccg
     5341 cccaggcaaa cggctacaac ggctccatgc gtgccatgac gcgtgaacag gctatcggca
     5401 tttaccgaaa ggcgttttgg gagcgttacc gcgccgacca aatgccggaa gcggtcgcgt
     5461 tccaattttt tgatgcctgc gtcaaccacg gttacggcaa tgccgtccgt atgctgcaac
     5521 gcgccgcagg cgtggcggac gatggaatca tcggcgaaat cagcctcaaa gccatcaatt
     5581 ccctccccga aaacgacctt ttattgcggt tcaacgccga gcgtctggtc ttttatacca
     5641 agctcagtac gttcacctct ttcggcaagg gctgggtacg ccgtgtggcg caaaacctga
     5701 ttcacgcgtc tgcagataac actgattaaa gggagacaaa ccatgtcaaa aaagtcactc
     5761 atcgccctaa tgaccgcagc catgcagccc gatttcagcc acagcgacct aggcattcgc
     5821 tacgccatgc cgactcaggg atgttggacg caagcccacc gcaagagcgg ggtagccgcc
     5881 gcgaaacgcg cagccaaaaa aaagcgtcgc aaataactgc ctttttccga tggctgggcg
     5941 gcttggtctc taatccggcc acaggaaaaa tcagccatac caaactatgg acaaacgtgg
     6001 cagccgcttc tatgacctat aagttctctc aaacagcaga tgcgccggaa tggctctggt
     6061 gggcttatgg cgcattggtc ggcgggtatg cattaatcaa acgcggcatc gccgccgtac
     6121 cgcagttggc agaaatcaaa aagtccgcga atgcggaaga atggagcggc aatgattgaa
     6181 tttgtccgag ccaaaaaacg gctgctttgg gcatttgtgc ttttgcttgt gtggacgtgc
     6241 ggttaccgat acgccgccga caaggccgaa gcggaacaaa ccgccctgat tgccacctat
     6301 cggcattctt ctatggttgc ggcggaacaa tatgccttgc agcttaaaaa agcgcaggac
     6361 gaaaggcagc ggtggtacga cttttcccaa aaacaaggaa gaaagcccgt gaaaaaacag
     6421 tatccgccgc aaacgaaaaa agccggctat ctgaaaacca aggaagaact gcttgcggaa
     6481 ttggcttgct ttacagcgga aatggctgcc ctaaaaaagc ccgatgcctt aatccatggg
     6541 aaagaagtgc ggcagaaaga acgcaactcg tcgcagggtt aaggcaatgc catccgttga
     6601 actgctgttg gagattgtcg gactgccacg cggcaccttt tattaccaat tggccgtcca
     6661 atcggcagaa gacaaatatg ccgatttgaa acagcatatc catgatattt atcgacgaca
     6721 taagggaaga tacggctacc ggaggattgc agcagccatc cttcatgcag gaacaccggt
     6781 caatcacaag aaagtcagcc gtctgatggc gaagacgggg ctgaaggcag tgatacggcg
     6841 gcgcaaatac cgctcgttca aaggagaagt cggcaaaatt gcgccgaata tcctgcgacg
     6901 ctgtttccat gcagaaaagc cgaatgagaa atgggtaacg gacgttaccg agttcaatgt
     6961 aggcggagaa aagatatacc tttctccgat tatggatttg tttaacgggg aaatcgtcag
     7021 ttaccgtatt caaatccgcc cgactttcga tttggccggc gagatactga aaggtgcgcc
     7081 ggagaaaccg ggatcgtctg aaaagccgat actgcattcg gatcaaggtt ggcaatatca
     7141 gatgtttttt tatagtggat taacaaaaat caggacaagg cgatgaagcc gcagacagta
     7201 caaatagtac ggcaaggcga ggcaacgccg tactggttta aatttaatcc actatacaac
     7261 aacgatagaa tcaagttgaa attaaaaggg ctgagccttg ttcagtacag aattcagtcc
     7321 ctgaaagccg cttgattaaa ctgtccgact ttttggggtc agttcggctt cggcattttt
     7381 ttatccgttt gggggtaact tgtttggaaa gctgcaagct tataaataaa ggattacatt
     7441 taagttttgg gtaacctttt taaaaaaatg cgtgatgact tttgcatttt taaggcgttt
     7501 tttggggtaa ttcgtgaaaa gttaccccaa aagttacccc ataaatggcg aaaactcaag
     7561 catacgccag catattcaag gcaacaaaaa agccttgaaa ctgttgaagt tcaaggcttt
     7621 tttgtgttgc aggatgctgc tgaaaatagg gtatggtgga ggcgggggga atcgaacccc
     7681 cgtccgaaag tcctctacaa agcgttctac atacttagtt gtgtctattt gaaaatctta
     7741 tttccatcat gccgaccaac aggccttttg gaaaccagtt accttaaatc tcatttcctg
     7801 ccaagtaacc cggtaggaaa ccagtcaatg taagatgacg ttgcggtggc tttcgccaca
     7861 cagcccattg accgactgct gcaacggcta gccttaagcg gctaaagcgt aagtttcgtc
     7921 gtttgcgact atttgaattc agtgttttac gggaatctga gaccccggta tgcccgcatc
     7981 tgcttcgcaa cccccgtcga aaccaaggtc gcccccagaa atggtttgca aattatacgg
     8041 atattgtgcg gtgctgccaa gtctgtcgga gaaatttgtc agtcttgttg ccttaatttg
     8101 cgtttgagca ggatgcggac gcagccgtcg ttgccttccc ggggttcggc gtaggcgagt
     8161 acgtcggggt gttgcatcag ccagtttcgg gtcatatttt tcagaacggg tttgtagcct
     8221 ttggaaccta atccgctgcc gtggatgatt tcgccgcata cgccgcgttt ttgggtgaat
     8281 gcgatgaatt cgttgaggac tttttgggct tcttcctgtg tgtagccgtg caggtcgaca
     8341 tcggtaacga cgggatagta tccgtttttc aggcgttgga tgtcgttttt tccctgtccg
     8401 tttttgctga agctggcggg cgggtcgttg tatgtgctgc cgatgtagaa gtagttttct
     8461 tcgtcggcgc ggttgtcttt gggacggact ttgatggggg ttttgtcggg cggcgcataa
     8521 tattgctgcc ggttttttaa tggggagact tgtccgactg cttgtgaaaa atcgaaatcc
     8581 tgttcttgtt tttgcttgtt tttttcggct gcctgttttt ctgccgcttc tttttgggct
     8641 tgtttgccca gttgtttgag gatgttttgg aagtcggtat tcatattttt tcctgttatt
     8701 tgtccgatgg ctgtttcggg cggggtttta atttgccgga atgtttgcca atcgggggag
     8761 gatgattttg ttgccgtcgt atgttttttg aaagtgtgat tgtatatcaa aaagaaatgc
     8821 ggcaaccgtc ggcagtgttg attgccggaa atgcgggccg gtcgaaccga tatgcccgaa
     8881 cgcctgataa agttttaaaa acctgccttg cgaagcaggc tgacgtgttt tgccaatttt
     8941 gaattgccgg aaacgcgaaa cacggaaatc tga
//
LOCUS       DAWFJW010000075         8962 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFJW010000075 DAWFJW010000000
VERSION     DAWFJW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.075"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(755..1117)
                     /locus_tag="WGR19_001467"
     CDS             complement(755..1117)
                     /locus_tag="WGR19_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0798975.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIEVNKLH
                     QKIVASSLLLRMLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     GFGRHYDTDENASSNIRK"
     gene            1537..2901
                     /locus_tag="WGR19_001468"
                     /pseudo
     CDS             1537..2901
                     /locus_tag="WGR19_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            3303..3767
                     /locus_tag="WGR19_001469"
     CDS             3303..3767
                     /locus_tag="WGR19_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798976.1"
                     /translation="MLHIPPTEPDRYVSSIVALNVHSSNGTGDWHSAAALNDDAYPQD
                     FYIYGKDQTRSTLHLLGNVGVIDGTNRFNEMGYYPENIPVWLADHPRACVDYLYTAVL
                     QTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWGNLQLWKRKNPIM"
     gene            3894..5183
                     /locus_tag="WGR19_001470"
     CDS             3894..5183
                     /locus_tag="WGR19_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEZ0798977.1"
                     /translation="MMKSILTVSGNRMRKPRITYLDVWANDERIGTLEKGAMYRFAYD
                     NPNSSLLGLHYQDRSKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILGRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IAVAQTSLSEDSSVLLVRRFDVSEQGYFLGMEDFTSLCQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPVYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(5508..7295)
                     /locus_tag="WGR19_001471"
     CDS             complement(5508..7295)
                     /locus_tag="WGR19_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEZ0798978.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHARF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPGYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     LGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWMRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLSGQTLVGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(7350..7808)
                     /locus_tag="WGR19_001472"
     CDS             complement(7350..7808)
                     /locus_tag="WGR19_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ0798979.1"
                     /translation="MPITPPLNIISPKLYPNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPFGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            complement(7974..8327)
                     /locus_tag="WGR19_001473"
     CDS             complement(7974..8327)
                     /locus_tag="WGR19_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798980.1"
                     /translation="MEKNMKQILFKYFMLITCVFGLSSCGYLLSFGQLMFDGSCADWN
                     CSKPTQRWAKAVDTCETENLTIVLQVLGEDLGYKSLVEVQVSEIGQHISGQCTYYDGR
                     YDVRKDKQYGHLFVQ"
     gene            complement(8391..8708)
                     /locus_tag="WGR19_001474"
     CDS             complement(8391..8708)
                     /locus_tag="WGR19_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0798981.1"
                     /translation="MKKTRSNMVLILFCSTILTGCFGSCGDWNCSGNPRWNEKVNDCY
                     SRNLEVVKQQLGYKKYGGDIVRFHSEVQDSEIGQHISTQCIYYSGKYDIHKDKQYGHL
                     FVK"
     gene            complement(8689..>8962)
                     /locus_tag="WGR19_001475"
     CDS             complement(8689..>8962)
                     /locus_tag="WGR19_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0798982.1"
                     /translation="NETTKGACKGFCYSHSSYFAEVPEQYQRDRNGEYILDKNNNKIE
                     TEDWKDYKGKWGVPKMGDDGKYINHAIPKLVYPNKLNGEQYEENPF"
BASE COUNT         2604 a         1875 c         1837 g         2646 t
ORIGIN      
        1 tatgacctta gttttagtat ccgtaatatc atcatttttt tcgtcaaggg aatatatcga
       61 ctccagaaga caggtattag atactgcctt ttcttacaag agtgatggta ggatggttct
      121 cttcaagtca atcaaacagg aaagtatttc ttttctgtct gaagatttga aaaaggactg
      181 gatgtttcac aaggttaaaa ctggggaaaa agatggatat ggttcagatg aaatgctgag
      241 cgtaccccgt gtctatttgg aaatgatgtc gcggaaaacg ggagtcccct actccagtat
      301 tctttaaatt ctaagcagaa gacttcttcg tcggtctttt tttgttgttt ggtttgcatg
      361 gagtaaaact gtacaactgt tgaagcataa aaccagcaag aaggtaaaaa agaaagaagc
      421 agtttttttg gattttagat gttaccgcaa ttagtttcct ttcctaaaat ttgtttaaat
      481 tatttgcaat attaatataa acgagatatt aatgatgaga aatcaaaaag gcataatgaa
      541 tatattttgt acaaaatatt tgcagtattt aaaaatgttg atttgtatat gaaaagttaa
      601 agatgccaaa atgtacagtt gctaaactgt aaaactgcta aagcaacaaa acataaaaag
      661 gaatgcaggg atgcaatcac tacatctttt tattccgtaa gcatttatga ctttacggtc
      721 aactgctact ctatgtttcc agcttttcag ctccctattt tcgaatattg gacgaggcat
      781 tttcatcagt gtcgtaatgc cggccgaaac cctcacaaac catattggtt cttgtggcag
      841 caacacctat ccgtttgttc aagcgaccac aagagtaaca tgattggctg gtggcattgg
      901 ctttaatctc ttcgatatga actccatttt tagctgcacc ttctttcagc atacgcagca
      961 gtagggatga ggcaactatc ttctggtgga gtttgttgac ttcaatctgt atgcactgta
     1021 ggttgagcag attcaatttt ttgatacaga ttatccggct ttgttcggca attctgttgg
     1081 cgaacgtata gtagagctgt cgtcttgcag ctttcaattt gcggtaggta ttttaccatt
     1141 caatgcgtag ccgctcggta cgtttgagcc aaatgttatc ttcgccattt gtaccaattg
     1201 gtttttacat tcggctgtgt tttagtaatt tattaatttc aattatttgc aagggaaaag
     1261 acaattgttt ccggttagga ataaacctat cctattgaat atattgaagc caagtacgtc
     1321 tatcaacact atattaaaac acagcctttt ttaatatagt agacacaatc tttccctatt
     1381 tatgaaggtg atcgcttctt tcagattcgt attttaatac tttctatttc tagaaaattt
     1441 gactaaaata actcaattat aaaaaattgc gcgattttgg tatttatcat gaaaatttcc
     1501 agacctccgg aatttaccct gttgcaacag gaatatatgc agcatctcac tgaaagaatg
     1561 acgcaaattg ccaagctgct gaattcttcc ccaaacaatc ctgatataga cattcccgat
     1621 tttcttactg aaatcaaaga ttattcagaa ttttccgtga cagatgaaaa tggaacctac
     1681 ctgcattggg acaaattccg ccggattcac acggaagata cgcggatgaa atggcgcgcc
     1741 gttaaggaaa gccgcaaaaa aatccaaaaa ccaattgatt tcccgtttga acatcagttt
     1801 tggttctgca ttcccgactc tttgcaggca cggcttcatt tgattgacaa aagctgcagc
     1861 agttctatcg gcacgtctag cttgggtggt ttcggcagaa gcgagcaaaa cagattcttg
     1921 ctcaagtctc tgattatgga agaagcgatt acatccgccc aactggaagg tgcggctacc
     1981 acgcgtaaag tggccaagga tatgctcaaa tcgcagcgta aacccaaaac aaaagacgaa
     2041 atcatgatag tgaacaacta tcacttgatg aaaaaagcgg tagaattgaa aaatacgccg
     2101 ttaagtgttg aaatgatttt ggatttgcac cgcattgcta ccagcaacgc tattgaaaac
     2161 aaggcctagc ccggacaatt caggcagtat gacgaaatct ttatcgccga tatcaatggt
     2221 aacagcctgt atcaaccacc gccgcacgga caggttcata cgctgatgga agaggtgtgt
     2281 gcgtttgcca ataataccta tgacggcgtg gaaaatccgt ttatccatcc ggttgtccaa
     2341 gctattatcc tgcatttcct catcggctac atccacccat ttggtgatgg caacgggcgg
     2401 acagcgcggg ctttgttcta ttggtttatg ctcaaaaacg gctactggct atttgaatac
     2461 atatccatca gccgtcttct gaaaaacgct cctgcccaat acgccaaatc ctatttgtat
     2521 gcggaaactg acgatttaga tttaacctat ttcatctatt accaatgcga tattatcaag
     2581 cgggcggttg ctgatttgga gcactacatt tctgacaaac aaaaacacca acaggaattc
     2641 aaagcagcga ttgcccaata tactgaaaag ataggaaagt tgaaccaacg gcaaattggt
     2701 atcctgcaaa aagcagtgga agaaagcgga aaaatcttta ctgcacaaga aattgccaac
     2761 caatacggca tctccctgaa tactgcccgt agcgatttga gcaaactggg agaatataga
     2821 ttcctagtgc cgttcaaatc aggaaatgct ttagagtatg ttgctcctca ggatttattg
     2881 gaaaggttag aaaaaaaata gtttgctagc ctaaaatgca gctttaaccg agtcaaaatc
     2941 aatacagtcc gcaccttcaa aaagaagctg cggactgctt gctttttgct ctacaaatga
     3001 tctttgtagc tgatttaacc aagattgtag caattttgct ttccgagcaa gcagggttag
     3061 aaaattcgat acttttaatt attggctgtg ttttaatatg atgttgatag gcgtgtttgg
     3121 ctttaaggta ttcaacagga taggtttatt cctaaccgga aaataattgt cttttccctt
     3181 gcaagtaatt gaaatcaaca gattactaaa acacagcctt acattattgg ggtgactatc
     3241 ctgtaaaata tgtcctaaaa cgtggaaacc acttttgctc tgctaaattt taaggaatct
     3301 ttatgttaca tataccccca acggaacctg atcgatatgt tagcagtatt gtagccttaa
     3361 acgtgcatag ttctaacggt acaggcgatt ggcacagtgc agcagccctt aatgatgatg
     3421 cttaccccca agatttctat atttatggca aagatcaaac acgtagcacg ttacatttgc
     3481 ttggtaacgt tggtgtgatt gatggaacaa accgcttcaa tgagatgggc tattatcctg
     3541 aaaacatccc agtttggctc gcagatcacc cccgtgcttg cgtggattat ctttacacag
     3601 cagtgttaca aactggctca atcggtcggg tgattttaga tgattggttt ccaagcgatg
     3661 aagacaagca atcagtttat gacttactta accaaatcga accgcacttg aatactcaag
     3721 aatgggggaa tttacagcta tggaaacgca aaaacccaat aatgtaatgc taaccgaacg
     3781 agataaaagg cacgagaagc ggtattgctg aatttcatcc gcaatacgcc atatacaatt
     3841 aaatcagcag atgtgtctaa acgtcccgtt gttcccgttt gaaagaattt ttcatgatga
     3901 agtctatcct caccgtatcc ggaaatcgta tgcgtaaacc cagaatcacc tatttggatg
     3961 tttgggcaaa cgatgaaaga atcggtactt tggaaaaggg agccatgtat cggttcgcat
     4021 acgacaatcc caattcttcg ttgctgggcc tgcattatca agacagaagc aaggtatata
     4081 tcagcaacaa catgccgcat atctttgcac agtattttcc ggaaggcttt ttggatgcac
     4141 acatcacaag caaatatgct tttcatgatg cgccttttga agacaatgag atgctgcgct
     4201 tggcaattct gggcagagag actttgggtc ggatacatgt gcgctgtaat gacccgcttt
     4261 ttaatgaatg gatcgacggg ttggagatga aaaatccaag aatattgact gaacgggatt
     4321 tgctgggcat aaatgcccga caggtttttc agcaatatat ggcagaaatc ttccatcacg
     4381 gccgtttcgt cagtgtatcc gggatacagc agaagatgtc cttagatgcc atcagcagaa
     4441 ataccaagca aactgcctca tatattgcca aaggttttga tgcatccgaa tatccttgct
     4501 tggctgccaa tgaattttta tgcatgcaaa ccatcaaaca agccggcatt gccgttgcac
     4561 agaccagcct gtcggaagat tcatcagtct tattggtacg tcggtttgat gtcagtgaac
     4621 aggggtattt cttaggcatg gaagacttta ccagtctgtg tcagtattca gcagaagata
     4681 agtataaagg cagttatgcg gctattgcac agattatccg acagatatcc ggcagaccag
     4741 atgaagattt aatccatttc tttaatcagc ttgctgccag ttgcatattg aaaaacggcg
     4801 atgcacacct caaaaatttt tcagtactct atcatgacga atacgatgtt cgtcttgcac
     4861 ctgtctatga tgtattggat acatcaatat acagggttgg aacacaagga atttttgatg
     4921 cttatgacga tacgctggca ttaaacctga ctaaccacgg taagaaaaca tatccttcca
     4981 agaatacatt gttggatttt gctgagaaat attgcgattt gggaagagaa gatgcatcct
     5041 ttatgataga tacaatcgtt caagctaaag aacaggttct tgttaaatac tcggatgtat
     5101 tgcgtgagaa tgaatggttg gcgcagaagt ggcattttat cccggatgaa aatgaagaag
     5161 gtctaccgtt tacattccgg tagctgccgc tgtcagagat ggccggtcga ctttcaccct
     5221 gaaaatcact tcatcttatg gtgtttgaaa ccgagaaatt agaagaatcg tattcggtag
     5281 gagatatact gggaagattg gaaaactggt aaatcactct attgattgag ttggcggcct
     5341 atatgtttaa tgtaggcaaa cgagaaggaa tcatgtattt catgattcct tcttaaattc
     5401 ctgtgtcaat ctaatatcaa aacacagcca tctcttacca taatcatgat aggtgtttta
     5461 ttatgaaaag ctatatctat agttaccgtt gatttgacta tgccgtttta aaacgtatag
     5521 cctacctgaa aaccgcttgc ccatttcctt gattggaaaa attcgggctt tttcaatgcg
     5581 cggccggtaa atatatcgta atgcaggttg ccgccaagct ttatctgccc gcgtatccca
     5641 attgctgtgc cgactagagt ttggcccgat aaccatttgg cggattgtcc tgaaacatgt
     5701 cctacatcag ccccaagata aagctgatgg cctggtttaa attgccagct caaatcgttg
     5761 cgccaatacc atccccgctc ggcagacaaa ctcatttcac cgtcgaagcc acgtacggtg
     5821 tggtgtccgc cgatagccag tttgtcttgc gatgttagcg gggttttgtt ccattgtgca
     5881 tgaacggatg tgtcataggc aaatagctgt ttaccgattt gaaaaggagt atttacatca
     5941 gccgatgccg tccaaatttt catacgtgac gtgccttcgc caaaggcttc ttcaggcgcg
     6001 cgcagagcat ctttcatgcc ggtgccgcgt ttatatttca acttaaaatc tgccgtactg
     6061 cgaccgatat attctttgtg ggaaagttct gccaaccaac ccgcagtttt acgccgttgt
     6121 acagtcagtt cggcatcatc aatgtaactt tttgtttccc tcatccacag ttttacaccg
     6181 agataggttt tgcgtttggc atcacgatac aacaggcggt tgaagccgaa atcagtattg
     6241 taactttttc cattatagtc atagacttcc gataatccgg aaactgcctg atggtaacgg
     6301 tagccattgt gattgaatgc ccatgtccat ttaccgaaag gggctgaata atgtacggcg
     6361 taattgtttg atccgccttc tttgcgatgg ccgtcaaaac tttcctcatc gggcgtaccg
     6421 ccaattgaac gtccataatt tacatagaac atatcactca gtcccaaagg attgtcggca
     6481 gagaaagtga tatttccttg gtattttcct gtcgcctcac tacccgaatt atccatcccc
     6541 acactcacac ggtagggcag cagacgttgc cgccattgca ccacgacatc actttggttt
     6601 ggttctccct ctacgggaac gatttggaga tcggcttccg cagtcgggag acgtttgaga
     6661 ttttccagtc cttgttccaa atcacgcaga ttcaacagat cgttcgagcg ggtgggaaat
     6721 ttgttctgga atgctgcaat acgtcctgca tgggtttgat catcgttaga ccgatcgatt
     6781 cgtatggagc gcagatagcc cggtatcagg gttaattgaa gcttgccact attcaaatcc
     6841 tgtggcgcag ccaagatacg ggtcgtggta tatcccctgc cgatcaaagc attttgtgct
     6901 aaggacatga tttgattaat gttgcccgca tgcagacact tgccagcctg aaaacccgtt
     6961 tcgcgcaagg cacgttttag ggcaaactga aaccgagcat ggtgttcgcc ttccaacacc
     7021 acttcgttaa tggcaaaaca cggttggctg ctgtcatcgc ccatcaactg attaaccgtt
     7081 tctcccgtgt ttttttgatg caaacgcaca tcgctttcag gctgcatggt ttggcgcaac
     7141 tgctcttcgc gttggcgttg ctgaatatct tgctgcatac ggatttcggc agggttgggg
     7201 gaggccaaca aagtagcagg agcaatgata cctgccaata agcagcacca agacaaaaag
     7261 cgaatattag gcaaatagga taaaggaagt ttcatggcat ggtcgcaaaa tcaataaaat
     7321 catcaatggg cattcaatac acgattgcat caagttttaa aagttaaaat tctcaaaccc
     7381 ttatcgctcc ctttatgata caaggcgcgt actgtcgtac taggaaataa ctggcgcaca
     7441 gctgagcgca tttgatgact gtgtccaaat ggcgcaaacc attgaatcag ccaaatattg
     7501 tcgccacagt tccaatcgct gttgtcacgc aaatggcggt cagattctaa ataatgcgcc
     7561 tgcgccactt catcaaaata agcccatgag atataaccga ttggttgggt acccttgcaa
     7621 aacaaagcga actgcccgtt ttttaacaca ggcaatatat acgtcatcat ctccacaata
     7681 ggtacttggc gatgcgtagg cgactgatac catagccaag tgatggcacc gagtgcttcg
     7741 ctttcgttcc attgttcatt ggggtagagt ttaggggaga tgatgtttaa gggtggagtg
     7801 atgggcatat tttaagttag ggttcgtgtt ggttaaaaca aaaaatggtt tcagcctgaa
     7861 atgaaatatt tcacgttaaa acatagtttt agcacatggg actgaactgc gtgggcaacg
     7921 agttgcccac cctaccatgt aacagttcgt attaattgca caggctacgc tggttattgc
     7981 acaaacaaat gaccatactg cttatctttg cgaacatcgt atctgccatc atagtaagta
     8041 cattgtccag aaatatgttg cccaatttca ctgacttgta cctcaacgag agatttgtat
     8101 cctaaatctt cccctaatac ttgtaacact attgtgagat tttcagtttc acatgtgtca
     8161 acggcttttg cccatctttg agtaggcttg gagcaattcc aatctgcgca actgccatca
     8221 aacattaact gcccaaatga taataaataa ccacaactgc ttaatccaaa aacacaagta
     8281 attagcataa aatatttaaa aaggatttgt ttcatatttt tctccattcg gattattggg
     8341 attgactaat tttggaatag cgtgatttat gtatttgcta tcattactag ctatttcaca
     8401 aacaaatgac catactgctt atctttatga atatcatatt taccgctata gtaaatacat
     8461 tgcgtagaga tatgttgtcc aatttcgcta tcttgcactt cgctatggaa acgaacaata
     8521 tcaccaccgt attttttata tcctaattgt tgcttaacaa cttcaaggtt tctactataa
     8581 caatcattaa ctttttcgtt ccatcgcgga ttacctgaac aattccagtc cccgcaacta
     8641 ccaaaacagc cagttaatat tgttgagcaa aacagtatca ataccatatt agaacgggtt
     8701 ttcttcatat tgctcaccat tgagtttatt cggatacact aatttcggaa tagcatgatt
     8761 gatatatttg ccgtcatcac ccattttagg aacaccccat ttccccttat agtctttcca
     8821 atcttctgtt tctattttgt tgttgttctt atcaaggata tattcaccat tcctatccct
     8881 ttgatactgc tcaggcactt ctgcaaaata actgctatgc gaatagcaaa atcccttaca
     8941 agctcctttt gttgtctcat ta
//
LOCUS       DAWFJW010000076         8768 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFJW010000076 DAWFJW010000000
VERSION     DAWFJW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.076"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(391..825)
                     /locus_tag="WGR19_001476"
     CDS             complement(391..825)
                     /locus_tag="WGR19_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0798983.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGR19_001477"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGR19_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0798984.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WGR19_001478"
     CDS             complement(1556..2398)
                     /locus_tag="WGR19_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0798985.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WGR19_001479"
     CDS             2653..3291
                     /locus_tag="WGR19_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0798986.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3742..4542)
                     /locus_tag="WGR19_001480"
     CDS             complement(3742..4542)
                     /locus_tag="WGR19_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0798987.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4678..5162)
                     /locus_tag="WGR19_001481"
                     /pseudo
     CDS             complement(4678..5162)
                     /locus_tag="WGR19_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5499..6140)
                     /locus_tag="WGR19_001482"
     CDS             complement(5499..6140)
                     /locus_tag="WGR19_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0798988.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            6469..8682
                     /locus_tag="WGR19_001483"
     CDS             6469..8682
                     /locus_tag="WGR19_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0798989.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAISKIRAYIRQQNAD
                     TVREEGRVQIDKQLAKLTPKPNLQELAENLGFKKLDDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT         2332 a         2351 c         1896 g         2189 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgct cagaaatgga gacggtgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccaatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccagtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgca gcaggcgcgc
     2221 ctgcaccttc gggggcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttca cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatcta gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg cggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tattttttca tcattattag aaccaggttg catgataata cctttcatta
     3421 actgaaacac tgattaagaa actccagtct gtctaatgat gaggttttca catcgccaaa
     3481 acttgccaat caaatgctgg atttattgcc gtctgagatt tggtcaaatc caaaggcgac
     3541 attcttagac cctgtgtgta aatcaggggt atttttgcgt gaaatcgtca aacgcttgga
     3601 tgaaggcttg accaatcaaa taccagataa acaaactcgc attaaccaca ttttaaaaaa
     3661 tcaagttttt ggaagtactg ccacgtatgt aggtagcttt gaccgatatt tgcataaaaa
     3721 ctcctttgct ggtgaaagga attattttgc caattttaaa atatttctgg caccaaatag
     3781 tacaatgaca aagacaatca tgccaatgat taaatcagga tagctagaat gagtcaataa
     3841 cgtcaatgct cccgccgcta tcacaccgat attgatgata atgtcattgg atgtaaaaat
     3901 catgctggct ttgatatgga tttctttatt ttgatttttg ctcagtagat ataagcacag
     3961 ccagtttgca atcaatgcca aaaatgccgt gccaatcatc agttgataat tgggcagctg
     4021 ctcagcaccg ataaaacgcc taatcacttc tatcacccca aataacgcca atattatctg
     4081 cgttatcccc gccaaaaatg ccacacgttt tttatacgcc agcgtcatac caatggctga
     4141 tagcgccaat atatagacaa agctgtccgc cagcatatct agactatcag caatcagccc
     4201 catagaatta gcaaaaatac caaccgaaca ctctatgata aaaaacacaa agttaatcat
     4261 gagcacttga tataataatc ttttttctaa gtgctcatca ggcttgttaa acactatctt
     4321 atcaacaatc acttcggtgg aaatgatatg actatcaaaa ttaagcggtt caagtacttg
     4381 taaaatcgtt gtatcttgat tatcgtgata gacggttaag caccgcccag caatatcaaa
     4441 ctgtaattca taaatatcag acacatcttt taaacgcatg cgaatgagct gttcttcgga
     4501 cgggcagtcc attttggtaa tgttaaaaat ggtctttttc atctatttag ttccttgttt
     4561 tgatcaggtt ggctcaaata aatctgtgtt tatattgctg cttggtaatt tttggatggt
     4621 ttgagtaaat tgattaggtt aaaatttacc tttggaagta ccgccacgca taatagttta
     4681 gatatgttta taatctctgg ataaaaaaac gtaataagtg cttactggat aacaaagtcc
     4741 aaaccaatag cagataaaat aaggcatcca ccccctttct tcattaagga taagagatat
     4801 cgagaaacaa atcataacta aacagaaaaa attttggaga taaagccatt tcattcccct
     4861 attcaagaat ctggccaaga taggtatttt gtattctaca aaaaagaaag gcatttccaa
     4921 gggaaacatg tcagataaaa acttttgttt attttttact atagatagaa ccttgcttct
     4981 caagagaaag ccattaatat atagagaata gtataagtat gaataatctt cattagacaa
     5041 aaagaagaaa tggcagataa attacatacg atatattgga atataaaata tttacggtct
     5101 aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca taaaaaaatc aaaggcgtcc
     5161 attaaactat ctttcacatt agaaatttaa aagctaaata atacgacaaa caatgtgaag
     5221 tactattcat ggtttattta aaaaataata ctattctgaa cattatttag atacagaaat
     5281 taacaaatta gaactaaaca agcttttaaa tactttaatt ttattggaaa gctataaaag
     5341 gaactataac tttacacact agtcacttct ttttaagagg caaaagggat tgggaaggtc
     5401 gtcttggaga taagcactgg tatttcggcc aatggtaaat agagtttacc tcaaataggg
     5461 tagaacctcc ttcatctgtc agttaataac agccactttt acaatgccct gtcaaaataa
     5521 agcggcacgc ccgatttttc actcatcgtc atcaaataac ccatcacctt ttggggccat
     5581 tcgatgccgc gcaccacggt cagattcctc aaaacgggga aaaccaaaat atcctccata
     5641 ccgattccgc cgttgatgcc gtctgaagca ccgtccatca aattctccaa ctcttgcaaa
     5701 tctgcgttta tccgttcgag gtattgggcg gttttattca aattggcgga aaagctgccg
     5761 atgcttttct cttttttgtc tgtaaaatat ttcaccgctt ccggcgttgc aaattcgggc
     5821 agcccgattt tgatcacgcg cggctgcacc agtttgtcgt tgtatccgcc caccttgtcc
     5881 agccacgccc gtatctcggg gcggacttcg tctttcagac ggtcttcgcg gtcgaaatgc
     5941 cgcacaatgt ccaaactctc gcccataaac gaaccgtctt ctttttgcag gacgggcact
     6001 tgtttcgcac cgatcatacc aatcggcgtt gcctcgtcgt cgtttgccag cacggcttct
     6061 tcaacgtccg cgccaaacag cccggcagcc atccgcgcac gcacacaaaa cgggcaatgg
     6121 tcgtaaatat acagtttcat caaaatattc ctcgtcaacc tgtcggtacc ggctacctta
     6181 acaccccgcg ccacccgaaa caagtttatc ttcccgccta tgcaccgtaa ataaataagc
     6241 tgagaccttt gcaaaaatag tctgttaacg aaatttgacg cataaaaatg cgccaaaaaa
     6301 ttttcaattg cctaaaacct tcctaatatt gagcaaaaag taggaaaaat cagaaaagtt
     6361 ttgcattttg aaaatgagat tgagcataaa attttagtaa cctatgttat tgcaaaggtc
     6421 tcaagctgtt acaataaact cgtttttatc ggaacggaag accccatcat gaccgccatc
     6481 agcccgattc aagacacgca aagcgcgact ctgcaagaat tgcgcgaatg gttcgacagc
     6541 tactgcgccg cgctgccgaa caacgataaa aaacttgtct tagccgcccg ttcgctggcg
     6601 gaagcacatt accccgccga tgccgccacg ccgtatggcg agccgctgcc cgaccacttc
     6661 ctcggcgcgg cgcaaatggt tcatgaactc gacctgctcc ccgatgccgt cgccgccacc
     6721 ctgcttgccg acatcggacg ctacgtcccc gattggaacc tattggtttc cgaacgctgc
     6781 aacagtaccg tcgccgagct ggtcaaaggt gtggacgaag tgcagaaact cacccacttc
     6841 gcccgggtgg acagcctcgc cacgccagaa gaacgcgccc agcaagcgga aaccatgcgg
     6901 aaaatgctgc tggcgatggt taccgacatc cgcgtcgtgt taatcaaact ggcgatgcgt
     6961 acgcgcaccc tgcaattttt aagcaacgcc cccgacagcc ccgaaaaacg cgccgtcgcc
     7021 aaagaaaccc tcgacatctt cgccccgctc gccaaccgtt tgggcgtgtg gcagctcaaa
     7081 tggcagctcg aagatttggg cttccgccat caagagcccg aaaaataccg cgaaatcgcg
     7141 ctgcttttgg acgaaaaacg caccgaacgc ctcgaataca tcgaaaactt cctcaacatc
     7201 ctgcgcggtg aactcaagaa atacaatgtc catttcgaag tcgccggccg cccgaaacac
     7261 atctactcca tttacaaaaa aatggtgaag aaaaaactca gcttcgacgg cctctttgac
     7321 atccgcgccg tgcggattct ggttgatacc gtccccgagt gttacaccac gctgggcatt
     7381 gtccacagcc tttggcagcc tatccccggc gagttcgacg actacatcgc caacccgaaa
     7441 ggcaacggct ataaaagttt gcacaccgtc atcgtcggtc ccgaagacaa aggtgtggaa
     7501 gtgcaaatcc gcaccttcga tatgcaccaa ttcaacgaat tcggtgtcgc cgcccactgg
     7561 cgttacaaag aaggcggcaa aggcgattcc gcctacgagc aaaaaatcgc ctggttgcgc
     7621 caacttttgg actggcgcga aaatatggcg gaaagcggca aggaagacct cgccgccgcc
     7681 ttcaaaaccg agctgttcaa cgacacgatt tacgttttaa ccccgcacgg caaagtcctt
     7741 tccctgccta cgggcgcgac acccatcgac ttcgcctacg ccctgcacag cagcatcggc
     7801 gaccgctgcc ggggcgcgaa agtcgaaggg caaatcgtgc cgctgtccac cccgcttgaa
     7861 aacggacagc gcgtcgaaat cattaccgcc aaagaagggc atccttccgt caactggctg
     7921 tacgaaggct gggtcaaatc cggcaaggca atcagtaaaa tccgcgccta catccgccag
     7981 caaaacgccg acaccgtgcg cgaagaaggc cgcgtccaaa tcgacaagca gcttgccaaa
     8041 ctcacgccca agcccaacct gcaagagctt gccgaaaacc tcggcttcaa aaaactcgac
     8101 gacctctaca ctgccgtcgg acaaggcgaa atttccaacc gcgccatcca aaaagcctgc
     8161 ggcacgctga acgaacctcc gcccgtaccc gtcagcgaaa ccaccatcgt caaacagtcc
     8221 aaaatcaaaa aaggcggcaa aaacggtgtg ctcattgatg gcgaagacgg tttgatgacc
     8281 acgcttgcca aatgctgcaa acccgcgccg cccgacgaca ttgtcggctt cgttacccgc
     8341 gatcgcggca tttcggtaca ccgcaaaacc tgcccctctt tccgacacct cgccgaacaa
     8401 gcacctgaaa aagtgctgga cgcaagctgg gcggcgttgc aggaaggaca agtgtttgcc
     8461 gtcgatatcg aaatccgcgc ccaagaccgc tccgggcttt tgcgcgacgt atccgacgcg
     8521 ctcgcccgcc acaaactcaa cgttaccgcc gtgcaaaccc agtcccgcga cttggaagcc
     8581 agcatgaggt tcacgctcga agtcaaacaa gttaccgacc tcccacgcgt cctcgccagc
     8641 ctcggcgacg tcaaaggcgt attgagcgtt acccggcttt aaatacaaaa atgccgtctg
     8701 aaagccgtat aacgcttcag acggcatttt gattgccggg gtttgctatt ttttgttgca
     8761 taatcaat
//
LOCUS       DAWFJW010000077         8763 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFJW010000077 DAWFJW010000000
VERSION     DAWFJW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.077"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            77..1252
                     /gene="ctrA"
                     /locus_tag="WGR19_001484"
     CDS             77..1252
                     /gene="ctrA"
                     /locus_tag="WGR19_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0798990.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            1267..2430
                     /gene="ctrB"
                     /locus_tag="WGR19_001485"
     CDS             1267..2430
                     /gene="ctrB"
                     /locus_tag="WGR19_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0798991.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     KSLRKEINQQLRAISGGGQSSLSNQAADYQRVYLENQLAEQQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            2430..3227
                     /locus_tag="WGR19_001486"
     CDS             2430..3227
                     /locus_tag="WGR19_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0798992.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            3224..3874
                     /locus_tag="WGR19_001487"
     CDS             3224..3874
                     /locus_tag="WGR19_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0798993.1"
                     /translation="MISVEHVSKQYQMRGGMRTVLDDINFSLQKGEKVGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            3939..6212
                     /locus_tag="WGR19_001488"
     CDS             3939..6212
                     /locus_tag="WGR19_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0798994.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIACRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPARRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRNENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEIGDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            6243..7245
                     /gene="dcm"
                     /locus_tag="WGR19_001489"
                     /pseudo
     CDS             6243..7245
                     /gene="dcm"
                     /locus_tag="WGR19_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7238..7582
                     /locus_tag="WGR19_001490"
     CDS             7238..7582
                     /locus_tag="WGR19_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ0798995.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            7625..8281
                     /gene="galE"
                     /locus_tag="WGR19_001491"
     CDS             7625..8281
                     /gene="galE"
                     /locus_tag="WGR19_001491"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0798996.1"
                     /translation="MAFTLMRRAMIRKMPYTEDMRPGDTANPYGASKAMVERMLTDIQ
                     KADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVFGDDY
                     PTPDGTGMRDYIHVMDLAEGHVAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAFEAAS
                     GLNIPYQIQPRRAGDLACSFADPSHTKQETGWETKRSLAQMMEDSWRWVSRNPNGYGD
                     "
     gene            8282..>8763
                     /locus_tag="WGR19_001492"
     CDS             8282..>8763
                     /locus_tag="WGR19_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0798997.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDGPAAF
                     IETNIVGTYTLLEAARAYWHDMPPEKKAAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     P"
BASE COUNT         2229 a         1978 c         2284 g         2272 t
ORIGIN      
        1 tactctacgt actcataaat ttatttcgat taagtgctat aattaggcca tttataatta
       61 tattaggatt tggcttgtgt ttaaagtgaa attttatatt cgtcacgcag tattattatt
      121 gtgtggaagt ttaattgtag gatgctctgc gattccttca tcaggcccca gcgcaaaaaa
      181 aattgtctct ttagggcaac aatctgaagt tcaaattcct gaagtggagc tgattgatgt
      241 gaatcatacg gttgctcagt tattatataa ggctcagata aatcagtcat tcactcagtt
      301 tggcgatggt tatgcttcgg ctggtacgct aaatattggt gatgtattgg atattatgat
      361 ttgggaagcg ccgccggcag tattgtttgg tggtggcctt tcttcgatgg gctcgggtag
      421 tgcgcatcaa actaagttgc cagagcagtt ggtcacggca cgtggtacgg tttctgtgcc
      481 gtttgttggc gatatttcgg tggtcggtaa aacgcctggt caggttcagg aaattattaa
      541 aggccgcctg aaaaaaatgg ccaatcagcc acaagtgatg gtgcgtttgg tgcagaataa
      601 tgcggcgaat gtgtcggtga ttcgtgctgg gaatagtgtg cgtatgccgc tgacggcagc
      661 cggtgagcgt gtgttggatg cggtggctgc ggtaggtggt tcaacggcaa atgtgcagga
      721 tacgaatgtg cagctgacac gtggcaatgt agtacgaact gttgccttgg aagatttagt
      781 tgcaaatccg cgacaaaata ttttgctgcg tcgcggtgat gtggttacca tgattaccaa
      841 tccctatacc tttacgtcta tgggtgcggt ggggagaaca caagaaatcg gtttttcagc
      901 cagaggctta tcgctttctg aagccattgg ccgtatgggc ggtttgcaag atcgccgttc
      961 tgatgcgcgt ggtgtgtttg tgttccgcta tacgccattg gtggaattgc cggcagaacg
     1021 tcaggataaa tggattgctc aaggttatgg cagtgaggca gagattccaa cggtatatcg
     1081 tgtgaatatg gctgatgcgc attcgctatt ttctatgcag cgctttcctg tgaagaataa
     1141 agatgtattg tatgtgtcga atgcgccgtt ggctgaagtg cagaaattct tgtcgtttgt
     1201 gttctcgccg gttaccagtg gcgcgaacag tattaataat ttaactaatt aatgtgagta
     1261 attaagatgt ctgagcaact tcctgtggca gttgccactg aaaccaaagc cgagcgtaaa
     1321 aagccgaaaa agaaaagttg gattaaaaag ctaagccctt tattttgggt aacggtgatt
     1381 atccctacgg taatttcgtt ggtgtatttc ggcttcttcg cttccgatcg ttttacgtcg
     1441 caatcgagct ttgtggtgcg ctcgcctaaa agccaatctt ctctcaatgg cctgggtgcc
     1501 attttgcagg gcacaggttt tgcccgtgcg caagatgata tttacacggt tggggagtat
     1561 atgcgttcgc gctcgtcttt ggatgaactg cgtaaaatct tgccggtgcg tgagttttat
     1621 gaaaccaaag gtgatgcgtt cagccgcttt aatgggtttg ggttccgtgg cgaggaagag
     1681 gctttttatc aatactataa aaatcaggtg atgatcaatt ttgatacggt ttcgggtatt
     1741 tccacgttga atgtaacttc ctttgatgcg ctggaatcta agaaaatcaa tgaggctttg
     1801 ttaaaacaag gtgaagcatt gattaaccag ttgaacgatc gtgcacgtgc tgatacggtg
     1861 cgctatgcgg aagaagtagt gaaaacggcg gcagagcggg taaaggaagc ctctcagaat
     1921 ctgacggatt accggattgc caatggcgtt tttgatttga aagcgcaatc ggaagtgcaa
     1981 atggggttgg tttccaagct gcaagatgaa ttgattgtga ttcaaaccca gcttgatcag
     2041 gtgaaagcag ttacgccaga aaacccgcaa attccaggtt tgcaggcacg tgagaagagc
     2101 ttacgcaaag aaattaacca acagttacgt gccatttcgg gtggtgggca atcatcatta
     2161 tcaaatcaag cagctgatta tcagcgcgtt tatttggaaa atcaattagc tgagcagcaa
     2221 ttggcagcgg ccatgacttc tctggaaagc gctaaagctg aagctgatcg ccagcagctt
     2281 tatttagaag tgatttctca gccgagcctg ccggatttgg cgcatgaacc taaacggtta
     2341 tacaacattg ttgccactct gattatcggc ttgatggttt atggtatttt gagcctgttg
     2401 actgccagca ttcgtgagca taaaaactga tgaaagcctt gcataaaaca tcattttggg
     2461 aatctttggc tattcaaagg cgcgtgatcg gcgcgctttt gatgcgggaa attatcaccc
     2521 gttacggtcg caataatatt ggctttttat ggctgtttgt tgaaccgttg ctgatgacat
     2581 tcgttatcgt cttgatgtgg aaatttttaa gggcagaccg atattcaact ttgaatattg
     2641 tcgcatttgc gattactggc tatccgatgt tgatgatgtg gcgtaatgcc tcaaaacggg
     2701 cagttgggtc gatttcttca aatgccagct tgctttatca ccgcaatgta agagttttgg
     2761 ataccatctt ggcgcgcatg attttggaaa ttgctggtgc aaccattgcg cagattgtga
     2821 ttatggcggt attgattgcg attggctgga ttgaaatgcc ggcagatatg ttttatatgc
     2881 tgatggcttg gcttttgatg gctttttttg cgattggttt gggtttggtg atttgttcga
     2941 ttgcctttaa tttcgagccg tttggcaaga tttggggcac attgactttt gtgatgatgc
     3001 cgttatccgg tgcgttcttt tttgtgcata atttgccgcc caaggtacaa gaatatgcat
     3061 taatgattcc gatggtgcat ggcacagaaa tgttccgtgc cggatatttt ggcagcgatg
     3121 taattaccta tgaaaatcct tggtatatcg tattgtgcaa tctggtgttg ttgttgtttg
     3181 gcttggcgat ggtcagtaaa ttcagtaaag gagtcgagcc gcaatgattt cggttgaaca
     3241 tgtttccaag cagtatcaaa tgcgcggcgg tatgcggacg gtgttagacg atatcaattt
     3301 ttcgctgcaa aagggtgaga aagttggtat tttgggtcga aacggtgcag gtaaatcgac
     3361 gttgatccgt ttgattagtg gtgtcgagcc gccgaccacg ggtgaaatca agcggacaat
     3421 gagtatttct tggcctttgg cattctccgg tgcgtttcaa ggtagtctga ccggtatgga
     3481 caatttgcgt ttcatctgcc ggatttacaa tgtcgatatc gattatgtga agaactttac
     3541 cgaagagttt tccgagttgg ggcaatatct ttacgagccg gtcaagcgtt attcttcagg
     3601 tatgaaggcg cgtttggcat ttgctttgtc gttggcggta gagtttgatt gctatttgat
     3661 tgacgaagtg attgcagttg gtgactcgcg ctttgccgat aaatgtaagt acgagttgtt
     3721 tgaaaagcgc aaagaccgtt ccatcatctt ggtgtcgcac agccacagcg ccatgaagca
     3781 atattgcgat aatgcgatgg tgctggaaaa agggcatatg caccagtttg aagatatgga
     3841 caaagcctac gaatattata attcgctgcc ttaaagcgat tgttttttaa atcaggccgt
     3901 ctgaaatttc agacggcctg tccgttggaa ttctattgat gaacattact caaattcttt
     3961 cccaagaact ttccgcgact accgcgcaaa tcaatgccgc tattgagctt ttagacgacg
     4021 gcgcaaccgt gccgtttatc gcgcgttacc gtaaagaagc caccggcggg ctggacgaca
     4081 cgcagctgcg ccggcttgcc gagcggctgc aatacctgcg cgagttggaa gagcgcaaag
     4141 ccgttgtgtt aaaaagcatt gaagagcaag gcaagctttc agacgacctc agggcgcaaa
     4201 tcgaagccgc cgataacaaa accgcgctgg aagacctgta tctgccctac aaacccaaac
     4261 gccgcaccaa agcgcaaatc gcgcgcgaac acggtttgca gccgctggcg gacgtgttgc
     4321 ttgccgagca gtcgcaggac gtggaagccg ccgcacaagg ctacctgaac gaaaacgtcc
     4381 ccgatgccaa agccgcgttg gacggcgcgc gtgcgattct gatggagcag tttgccgaag
     4441 acgcggaact catcggcacg ctgcgcgaca agctgtggaa cgaagccgaa atccacgcgc
     4501 aagttgttgg aggcaaagaa accgaaggcg aaaaattcag cgattatttt gaccaccgcg
     4561 aacctattcg tgcgatgccc agccatcgtg cgctagcggt tttgcgcgga cgcaacgaag
     4621 gcgtgttgaa catcgcgctc aaatatcagc ccgacgacac gccgattacg cagcaaagcg
     4681 aatacgagca aatcatcgcc tgccgcttca aggtttcaga cggccacaaa tggctgcgcg
     4741 ataccgtgcg tctgacttgg cgcgcgaaaa tctttttgtc gttggaactt gaagctctca
     4801 atcgtttgaa agaagccgcc gacaccgacg cgattaccgt gttcgcccgc aatctcaaag
     4861 acttgctgct tgctgcgccc gccagacggc tgaccacgct gggtctcgac cccggctacc
     4921 gcaacggcgt gaaatgcgcc gtggtggacg acaccggcaa gctgctggat accgtcatcg
     4981 tctatttgca tcaagaaaac aatatgttgg caacgctgtc gcgcctgatt aaacagcacg
     5041 gcgtgaagct catcgccatc ggtaacggca ccgccagccg tgaaaccgac aaaatcgcgg
     5101 gcgaactggt gcgtgaaatg tcgaaaatgg ggctgcataa aatcgtcgtg tccgaagccg
     5161 gcgcgtcgat ttattccgcg tccgaactgg cggcgcgcga gttccccgac ttggacgttt
     5221 ccctgcgcgg cgcggtgtcc atcgcccgca ggctacaaga cccgcttgcc gagctggtca
     5281 aaatcgaccc caaatccatc ggcgtgggcc agtatcagca cgacgtgaac caaagccagc
     5341 tcgccaaatc gctggacgcg gtggtcgaag actgcgtgaa cgccgtcggc gtggacgtga
     5401 atactgcctc cgccccgctc ttggcgcgga tttccggttt gaatcagacc cttgcccaaa
     5461 acatcgtcgc ctaccgcaat gaaaacggcg cgttcgacag ccgcaaaaaa ttgctgaaag
     5521 taccgcgttt gggcgaaaaa accttcgagc aggcggcagg ctttttgcgg attaacggcg
     5581 gcacagagcc gttggacgcg agcgccgtcc accccgaagc ctatcccgtc gtcgccaaaa
     5641 tgctggcgca acaaggcatt agcgccgccg aactcatcgg caaccgcgag cgcgtgaagc
     5701 aaatcaaagc gtccgacttc accgacgaac gcttcggcct gccgaccatt ttggacatcc
     5761 tgtccgaact ggaaaaaccc ggccgtgatc cgcgcggcgc gtttcagacg gcatcgtttg
     5821 ccgaaggcat ccacgaaatc ggcgacttgc aagtcggtat gattttagag ggcgtggttt
     5881 ccaacgttgc caacttcggc gcgttcgtgg acatcggcgt gcatcaggac ggcttggtgc
     5941 acatctccgc cctgtccaac aagttcgtcc aagacccgcg cgaagtggtg aaagctggcg
     6001 acgtggtgaa agtgaaagtg ctggaagtcg atgctgcacg caaacgcatc gcgctgacca
     6061 tgcgcttgga tgacgaaccg ggcggcgcaa aacataaaat gccgtctgaa aaccgcagcc
     6121 gcgaacggac agccggccgc aaaccccaac gcaacgaccg cgccccagcc aattcggcga
     6181 tggcggatgc gtttgcgaag ctgaagcggt aaaataatcg aagagtttat ggattttgac
     6241 ttatgcacac accacttacc tatattgacc ttttctcagg agcaggaggc ctatccttgg
     6301 gttttgaaca agccggattc caacaattgc tttctgttga aatggagtct gattattgtc
     6361 agacttaccg taccaacttc ccccatcatc aattactgca aaaagattta accacactaa
     6421 ccgaacaaga tttaatcaat tgtcttaacg gacaagcagt tgatttgatt attggaggac
     6481 caccttgtca aggttttagt atggcaggaa agattggacg gacatttaca gatgacccac
     6541 gcaaccattt atttaaagag tttgtccgaa tagttaaaat tgtccaacca tatttttttg
     6601 ttatggaaaa tgtagcgcga ctctatacac acaattcagg taaaacacgt attgagatta
     6661 ttcaagcatt tcagaatatc ggttattcgg tggaatgtaa gatactgagt gcagccgatt
     6721 tcggtgttcc tcagatacgt agccgagtga tatttatcgg gaggagggat aaaggcaaaa
     6781 tttcctttcc cgaacctttg cagatttccc atcagactgt tggatcagca ataggacatt
     6841 ttccaaaact ggctgctggc gaaagcaatc cacacgttgc aaatcatgaa gctatgaatc
     6901 attcggcaca aatgttagaa aaaatggcat ttgttaaaaa tggaggtaac cgtaacgata
     6961 ttcctgaacc attacgtccg aaaacaggtg atatccgtaa atacatccgt tacaacagca
     7021 acaaaaccag ccgtttgtat tacaggagat atgcgcaaag tttttcacta tgaacagaat
     7081 cgggcgttaa ccgttcgtga attagctgcc ttacaatctt tccctgataa ttttattttt
     7141 tgcggcagca aaattgccca gcagcagcag gttggtaacg ccgtaccgcc tttattggca
     7201 aaagctattg ctgaaagtat tttaaaaatg agtgaaaatg aataagcaat atccgaaaat
     7261 taactatatc ggtaataaag agaaaatagc ttcctggatt tgtgaccagc ttccgtctga
     7321 tgtagataca gttgcagatg tatttagtgg aggctgttcc tttgcctacg aagccaaaaa
     7381 acgcggctat cgtgtgatta ctaacgatat tttggcaatt aattaccaaa ttgctttagc
     7441 attaatagaa aacaaccatg aaacattaaa tgacgatgat gtcgcaatga ttttttcagg
     7501 cagcccgcat gccggtttta tgagtcagcg ttatgccgaa aaattctatt ttcacgatga
     7561 ataccaacaa cttgatttgt aacgtaaaaa tatagggaaa ctggataacc agtataaacg
     7621 cgctttggcg tttactttaa tgcgtcgcgc catgatacgt aaaatgccct atacggaaga
     7681 tatgcgcccg ggcgataccg ctaatcctta tggcgcatcc aaagcgatgg tggaacggat
     7741 gttaaccgac atccaaaagg ccgatccgcg ttggagcgtg attttgttgc gctatttcaa
     7801 cccgattggg gcgcacgaaa gtggtttgat tggcgagcag ccaaacggca tcccgaataa
     7861 tttgttgcct tatatctgcc aagtggcggc aggcaaactg ccgcaattgg cggtatttgg
     7921 cgatgactac cctacccccg acggcacggg gatgcgtgac tatattcatg tgatggattt
     7981 ggcagaaggc catgtcgcgg caatgaaggc gaaaggcggt gttgccggcg tacatttgtt
     8041 caacttgggt tcggggcgcg cctattcggt tttggaaatc atccgtgcct ttgaagccgc
     8101 atccggctta aatattccct atcaaatcca accccgacgc gccggagact tggcgtgttc
     8161 ctttgccgac ccgtcccata ccaaacaaga gacaggttgg gaaaccaaac gcagccttgc
     8221 gcaaatgatg gaggattcgt ggcgttgggt cagccgcaac cccaacggat atggggatta
     8281 aatgcaaacc gcaaacaaaa aaaccatcct cgttaccggc ggcgcgggct ttatcggctc
     8341 tgccgttgtc cgccatatca ttcaaaacac ccaagattcc gtcgtcaacc tcgacaaact
     8401 gacctacgcg ggcaatctcg aatcgctgac cgacattgcc gataatcccc gctacgcttt
     8461 tgagcaagtg gatatctgcg accgcgccga actcgaccgc gtattcgcgc aacaccggcc
     8521 cgatgcggtg atgcacttgg cggcagaaag ccatgtcgac cgcagtattg atgggccggc
     8581 ggcatttatc gaaaccaata ttgtcggcac ttatacgctt ttggaagcgg cgcgcgctta
     8641 ttggcacgat atgccgcctg aaaaaaaagc ggctttccgt tttcatcata tttcgactga
     8701 tgaagtgtac ggcgatttgc acggcacgga tgatttgttc accgaaacca cgccttacgc
     8761 ccc
//
LOCUS       DAWFJW010000078         8701 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFJW010000078 DAWFJW010000000
VERSION     DAWFJW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.078"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            192..1727
                     /locus_tag="WGR19_001493"
     CDS             192..1727
                     /locus_tag="WGR19_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0798998.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFAYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1724..1816
                     /locus_tag="WGR19_001494"
     CDS             1724..1816
                     /locus_tag="WGR19_001494"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0798999.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1876..3096)
                     /gene="lysA"
                     /locus_tag="WGR19_001495"
     CDS             complement(1876..3096)
                     /gene="lysA"
                     /locus_tag="WGR19_001495"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0799000.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDEDVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNISPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(3107..3277)
                     /locus_tag="WGR19_001496"
     CDS             complement(3107..3277)
                     /locus_tag="WGR19_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0799001.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3348..3671
                     /gene="cyaY"
                     /locus_tag="WGR19_001497"
     CDS             3348..3671
                     /gene="cyaY"
                     /locus_tag="WGR19_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0799002.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3698..4714
                     /locus_tag="WGR19_001498"
     CDS             3698..4714
                     /locus_tag="WGR19_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0799003.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4740..5159
                     /locus_tag="WGR19_001499"
     CDS             4740..5159
                     /locus_tag="WGR19_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0799004.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALRINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKST"
     gene            5181..5687
                     /gene="luxS"
                     /locus_tag="WGR19_001500"
     CDS             5181..5687
                     /gene="luxS"
                     /locus_tag="WGR19_001500"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0799005.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNRNDDLA
                     LDESLLNA"
     gene            5833..8628
                     /gene="polA"
                     /locus_tag="WGR19_001501"
     CDS             5833..8628
                     /gene="polA"
                     /locus_tag="WGR19_001501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0799006.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     ITTEAQFAALLDKLAQADAIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALSGIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         2036 a         2257 c         2292 g         2116 t
ORIGIN      
        1 aaaatttttc gttttcaagc cttcaccgct tgccatcagc gttaaatttt tttacgataa
       61 gcacatagat tgtaaacaat cggccacaag ccggtttgtt ttttcagaag acattatccc
      121 tgtcagacgc tatttctata tatttcgcct atcatggctt gtttttaata aataattcaa
      181 gaggtatcaa cgtgtctgat tccaagacga aagaacgcgc tacattcggt acacgccgcg
      241 cgtttatgat tgccgccatc gggtccgccg tcggcttggg caatatttgg cgtttcccct
      301 atattgcttt tgaaaacggc ggcggcgcgt tcatcctgcc ctatctggtc gcgcttctga
      361 cggcgggcat cccgctgctg ctgctcgatt atgccatcgg ccaccgttac cgtggttctg
      421 cgcccttggc tttccgccgc ctcggacgat ggtttgagcc ggtcggctgg tggaacgtga
      481 tgaccaatat cgtcatctgc atctattacg cggtaattat cggttgggcg gcaagctata
      541 cctattattc ggtcaacgcc gcctggggtg cggatccgca gggttttttc tttaaagact
      601 tcctgcaaat ggcgggcccg gaagccttgg gtttggattt tgtcggcaaa gtggccggtc
      661 ctttggcggg cgtgtgggtt tttaccgccg ccattatggc tttgggcgtg caaaagggcg
      721 tggcgcgcgc ctcgtcgttc tttatgccgc tgcttttggt gatgtttttg attatggtcg
      781 gcatttcgct gacgttgccg ggcgcggcaa agggcttgga cgcattgttt acgcccgact
      841 ggtcgaaact cgccgattcc aaggtctggg tggcggcata cgggcagatt ttcttttcgc
      901 tttccatctg cttcggcatt atggttacct attcttctta tttgaagaaa aaaaccgact
      961 tgggcggaac ggggctggtg gtcggttttg ccaacagcag ctttgaactg ctcgcgggca
     1021 tcggcgtgtt tgccgcattg ggctttatgg cgcaggcggg cggtaaggcg gtcaacgagg
     1081 ttgcctcagg cggcatcggt ttggcgttta tcgcctttcc gaccattatc aaccaggcac
     1141 cgatgggctg gctgatcggc atattgtttt tcggttcgct ggtattcgcc ggcgttacgt
     1201 cgatgatttc catccttgaa gtgattgtgg cggcgattca ggacaagctg aacatcgggc
     1261 gcgtcaacgc cacgctgctg gtctgcattc cgatgggcat tgtttccacg ctgctgttcg
     1321 gtacggcgac ggggctgccg gttttggacg tgatggacaa attcgtcaac acctacggca
     1381 ttgttgccgc cggctttgct tatgttgccg ccatcatcat cagcggcagg ctgccggaat
     1441 tacgcaagca cctgaacgct ttgtcctcca tccgcatcgg cggcttgtgg acggtctgcg
     1501 tcgtggttac cgtcgtgatg ctcggctata tgctgtttaa agataccagc ggcctgatgg
     1561 agaaaaatta cgaaggttat ccggatggtt tcctcagtat tttcggctgg gggatgtcgg
     1621 cggcgttggt cgtgttcggg ctgctgctgt cgttgctgcc ttggaaacac ggtcaggatt
     1681 tcaacgtcaa agacgaacac gaacatgaac aaggagaaga aaaatgagta cttccgccat
     1741 tgtgatgatg attgtctcaa tcgtgataat ctggggaggg ctgctgcttt ccctgttaag
     1801 gctgccgaac gagtaagcct ttagagcgtt aaaaatgccg tctgaactgt ttcagacggc
     1861 attttgatgt tcggcttaca ggcaggcgag ttcgtttgcc atttgctgtt ctaaggtttc
     1921 gcgtcggcgg atgagtttgt atccgccacc gttaaccaac acttccgccg cacggttgcg
     1981 cgcgttgtaa ttgctcgcca tactggctcc gtatgcgccc gcgctgcgga taagcagcaa
     2041 atcgccttct tcgcaggcga tggtgcggtc tttgccgagg aagtcgccgg tttcacaaat
     2101 cgggccgacg atgttggcgg tcagaggcga gatgtttttg gtttcaaccg cttcgatgtg
     2161 gtgataggca tcgtataggg ccggacgcat taaatcgttc atcgccgcat cgaccatcac
     2221 aaagtttttc tcttcgccgt gtttgacaaa ctcgacgcgt gtcagcagcg aacctgcgtt
     2281 gccgaccagg ctgcggccgg gctcaagaat gagtttcaga cggcgtgtgc cgatcagttt
     2341 ttgaacggct tgggcatacg cgcccaaatc aggcacatct tcgtcttggt aaacaatgcc
     2401 gacgccgccg cccaagtcca agtgttccaa cacaatgcct tcggcggcaa gcgcgtcaac
     2461 caaaatcaaa atgcgctcgc aggcttcgac cagcgggctt aagtcggtta gttgggagcc
     2521 gatgtggcag tcgatgccga tgattttcaa attgggctgt tgtgcggcat ggcggtaggc
     2581 ttcgagcgcg tcggcgtagg cgataccgaa tttgttggct ttcaaacctg tggagatgta
     2641 aggatgggtt ttcgcatcga catcgggatt gacgcgcagg gagacgggcg cggttttacc
     2701 caaacgcgcg gcaactttct gaatacggtc gatttcgggg atgctttcca tattgaagca
     2761 tttcacgcct gcattcagcg cgaactcgat ttccgcctcg cttttgccta cgcctgaaaa
     2821 tatggttttt gccgcgtcgc cgcctgccgc caaaacgcgt gccaattcgc cgccggacac
     2881 aatgtcaaaa ccgctcccca aagaagcaaa gtgtttgatg atgctcaggt tgccattggc
     2941 tttaaccgca tagcagacga gcggggagag ctcggcaaag gcagtttggt agtgttcaaa
     3001 tgcttcggtc agcgcggatt ggctgtacac ataaagcggt gtgccgaatg cttcggcaag
     3061 gcgggggtag gggacttgtt cgcaaaatag ggtcatattt tcgttttcat ttttgggttg
     3121 gaggggcgga ttgcggtttg ccttgaagct gcaaaccggt ttggattacg ccgaaacgcg
     3181 ccttgtcgcc ttctttgggc aggtagaggt cgcctttgta accgcaggcc gagagcagga
     3241 gggcggttgc cgccgcaaaa aatacgccgt atttcatcgg taaacttcct tcataagcgc
     3301 gaatgtggca agattcggca tcttaaacaa aaaacacgca aaaagctatg atgaccgaaa
     3361 gcgagtttat ccgcgcgagc gaagcattat ttgaacacat cgaagaccaa atcgacgaaa
     3421 acggctggga tttcgactgc cggtttgccg gaaacgtcct gaccatcgaa gccgaaaacg
     3481 gcacgcaaat catcgtcaac cgccatacgc ccaaccaaga gttgtggatt gccgccaaaa
     3541 gcggcggcta ccatttcgcc gagcaaaacg gcaaatggct ggcaacacgc gacagccgcg
     3601 atttctacga cgttttaaac gaagcactga gcgcggcttc gggcgaagcg gtagagattg
     3661 aggaattgtg atttgggtgt tatcacggaa agaaaaaatg aacacacgtc ccttttattt
     3721 cggacttata tttatcgcga ttatcgctat acttgctaac tatttaggaa acactgattt
     3781 ttcccatcat tatcatatca gtgctttaat tattgctatc ttgctgggaa tggcaatcgg
     3841 caataccatt tatccgcaat tttcaacaca agtggaaaaa ggcgttttgt ttgccaaagg
     3901 cgcgcttctt cgcactggca ttgtgctgta tggtttccgc ctcacttttg gcgatattgc
     3961 cgatgtagga ttaaatgctg tcgttaccga tgcaatcatg ctaatttcaa ccttcttttt
     4021 taccgcactt ttaggcattc gttatctaaa aatggataaa caattggttt atctcactgg
     4081 ggcaggttgc agcatttgcg gtgcggcagc agtgatggcg gcagagcctg ttaccaaagc
     4141 agaatcccat aaagtttcag tggcgattgc cgtagtggtc attttcggga cgcttgctat
     4201 ttttacttac cccttgttct acacgtggtc acaacattta attaacgccc atcaattcgg
     4261 tatttatgtt ggttctagtg tacacgaagt ggctcaagtt tatgcgattg gggaaaatat
     4321 tgatcctatc gtggcgaata ctgccgtcat ttccaaaatg atccgagtga tgatgctcgc
     4381 ccccttttta ttaatgcttt cttggttatt aacacgtagt aatggagtat cagaaaatac
     4441 atcacacaaa attacaattc cttggtttgc tgtacttttt attggtgttg ccatttttaa
     4501 ttcttttgat ttattaccaa aagaactcgt gaaattattc gttgaaatcg attctttctt
     4561 attaatttca tcaatggctg cgcttggctt aacgacgcaa gcaagcgcaa tcaaaaaggc
     4621 aggattaaaa ccgtttgttt taggaatact aacttattta tggctagtgg ttggtggatt
     4681 tttagtgaac tatggaatat caaaattaat ataaaattca ctaaagagag cgttacccaa
     4741 tggcacaatt accgctatat ctgacttctg aaatcaaaga ctttactgtc ggcacgccta
     4801 aagttttaga atcattttcc aaacatatcc cttatggtgt tgtctttgaa gatgatggca
     4861 acacgggcta cttctatgcc gcttcgcaag acgggattct agatgccttg cacatctaca
     4921 atgtcgaaga tgtatccgac aaacatatcc ccaatcatgt cttgatttta tgggatgatg
     4981 cctgcaccat agccgcattg cgtatcaacg actacattca tgccgtctat gattttgtcg
     5041 aacaggcagg atattgccgc aacggcttcc ctgaagcagg cggcgaatgg gtgaaagtcg
     5101 aaaaccgcgt cttggacgat gaattgctgg acaaaatcct atcccgaaaa tctacataac
     5161 ctactcaaaa ggataacaaa atgcccctac tagacagttt caaagtcgat cacacccgta
     5221 tgcatgcccc cgccgtacgc gtggcgaaaa ccatgaccac gcccaaaggc gacaccatta
     5281 ccgtgttcga cctgcgcttt tgcgttccca acaaagaaat cctgcctgaa aaaggcatcc
     5341 acacgctgga gcatttgttc gccggtttta tgcgcgacca cttgaacggc aacggcgtgg
     5401 aaatcatcga catttccccg atgggctgcc gcaccggttt ctacatgagc cttatcggca
     5461 cgccttccga acagcaggtc gccgatgcgt ggctggcttc gatgcaggat gttttgaatg
     5521 tcaaagacca aagcaaaatc cccgagttga acgaatacca atgcggcact tatcaaatgc
     5581 actcgcttgc cgaagcgcag caaatcgcgc aaaacgtgtt ggcgcgcaaa gtggcggtca
     5641 accgaaacga cgatttggct ttggatgaaa gcctgctgaa cgcctaatcc gccaaaaatg
     5701 ccgtctgaac aagggtttca gacggcattt gccttttccg ttataatccg gggttgtgcg
     5761 ggggcgggtt ttaagccggc atcgtccttc cctatttttt tctgtccctt atcggtttta
     5821 agcgggtttt ttatgtccaa cagacctaca ctcctcctcg ttgacggctc ttcctatctc
     5881 taccgcgcgt atcacgcgat ggggcaaaac ctgaccgccc ccgacggcgc gccgacgggc
     5941 gcgctgtatg gcgtattgaa tatgttgcgc cgtttgcggt cggaatatcc gcacgattat
     6001 tgcgcggtgg tttttgatgc gaaaggcaaa aatttccgcc atcaaatgtt tgaagaatac
     6061 aaggcgacgc gcccgccgat gcccgacgat ttgcgcccgc aggcggaagc actgccggat
     6121 ttagtgcgcc tgacgggctg gccggtattg gtgattgggc aagtggaggc ggacgatgtg
     6181 atcggcacgc tggcgaaaca gggggcggaa catggtttgc gagtcattgt ttcgaccggc
     6241 gataaggaca tggcgcagtt ggtggatgag cgcgttacgc tggtgaacac gatgagcgga
     6301 gaaacgctgg atattgaagg cgtgaaggca aaattcggcg tgcgccccga ccaaatccgc
     6361 gattatctcg cgctgatggg cgacaaggtg gacaacgtgc cgggcgtgga aaaatgtggc
     6421 ccgaaaacgg cagtgaagtg gctggaagcc tacggctcgt tgcaaggcgt gatggaacac
     6481 gcttcggaaa tcaagggcaa agtgggcgag aatctgcgtg ccgccctgcc gcgcctgccg
     6541 ctgtcgtatg atttggtaac gattaaaacc gatgtggact tgcacgccga gctttcagac
     6601 ggcatcgaaa gcctgcgccg tactacgccg aaatgggcgc agctggttgt cgatttcaaa
     6661 cgctggggct tccgcacctg gctgaaagaa gcggaatcaa acatgaatac cggctcgacc
     6721 gatgatttgt tcggcagcga cagcatcggc gagcaggcgg ctttgaatgc ggaaatgccg
     6781 tttgaaaaac aagccgaaaa agccaccgcc cccgaaaagc tggattatca agctattacc
     6841 accgaagcgc agtttgccgc cttgttggac aaactggctc aggcagacgc aatcggcatc
     6901 gatacggaaa ctacgtcatt agacgcgatg aacgccgcgc tggtcggcat cagcattgcg
     6961 ttccaagcag gcgaagcggt ttacatcccc gtaggacaca gcctgaccgc cgcgcctgaa
     7021 cagcttgatt tgcaagacgt attaggccgt ctgaaaccgc atttggaaaa ccccgccctg
     7081 aaaaaaatcg ggcaaaacct caaatacgac caacacgtct tcgccaacta cggcatcgcc
     7141 ctgagcggca ttgccggcga cgccatgctc gcttcctaca tcatcgagag ccatctggga
     7201 cacggcttgg acgaattgtc cggacgctgg ctcggcttgg aaaccattac ctacgaatcg
     7261 ctgtgcggca aaggcgcgaa acaaatcggt tttgctgatg tcgccatcgg gcaggcaacc
     7321 gaatacgccg cccaagatgc cgatttcgcc ctgcgcctcg aagcgcacct gcgcgcgcaa
     7381 atggacgaaa aacagcttga aatgtatgaa aaaatggagc tgcccgtcgc gcaggtattg
     7441 tttgaaatgg aacgcaacgg tgtgcaaatc gaccgcgccg aactcgcccg ccaaagcgcg
     7501 gaactcggcg ccgagctgat gaagctcgaa caagaagcct acgccgccgc agggcagccg
     7561 ttcaacctca actcgcccaa acagctgcaa gaaatcctgt tcgacaaaat gggcatcccg
     7621 acaaaagggc tgaaaaaaac cgccaagggc ggcatttcca ccaacgaggc cgtgctcgaa
     7681 cagctcgcgc ccgactaccc cttgcccaaa atcatcctgc aaaaccgcag cctggcgaaa
     7741 ctcaaatcca cctacaccga caaactgccc gacatgattt ctccccggga taaccgcgtg
     7801 cataccacct acgcccaagc cgtcgccatt acaggacgct tggcaagcaa caaccccaac
     7861 ctgcaaaaca tccccatccg caccgccgaa ggccgccgcg tgcgccgcgc ctttaccgcg
     7921 ccaccgggca gcgtcatcgt ttccgccgac tattcccaaa tcgaactgcg catcatggcg
     7981 cacctctctg gagacaaaac cctcattgcc gcattccaaa acggcgaaga cgtacaccgc
     8041 cgcaccgccg ccgaagtgtt cggcactgcg cccgaaaacg tctcgcctga gcaacgccgc
     8101 tacgccaaaa ccatcaactt cggcttaatc tacggtatgg ggcaatacgg ccttgccaaa
     8161 tcattgggca tcgacaacct ttccgccaaa aactttatcg accgctactt cgcccgctac
     8221 cccggcgtcg ccgaatacat gcagcgcacc aaagaacaag ccgccgccca aggcttcgtc
     8281 gaaaccctgt ttggccgccg cctctacctg cccgacatcc gcaacaaaaa cgccaacgcc
     8341 cgcgccggag ccgaacgcgc cgccatcaac gcccccatgc aaggcaccgc ctccgacctt
     8401 atcaaacgcg ccatgataga cgtgtcccgc tggctggttt cagacgacct gaagagcaaa
     8461 ctgattatgc aggtgcatga cgaactggtg ctggaagtcg ttgaaaccga actggatttt
     8521 gtcaaagaaa aactgccgca gattatggcg aaagtgggcg gcggattatt ggatgtaccg
     8581 ctggtggctg aggttggcgt aggggagaat tgggaagagg cacattgatt gaaaggtgtt
     8641 attatgctat ctttatttaa ataaaattta atttttggta tattttttct aaatgttcct
     8701 a
//
LOCUS       DAWFJW010000079         8621 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFJW010000079 DAWFJW010000000
VERSION     DAWFJW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.079"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..244)
                     /locus_tag="WGR19_001502"
     CDS             complement(<1..244)
                     /locus_tag="WGR19_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799007.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQ"
     gene            complement(367..1716)
                     /gene="gshA"
                     /locus_tag="WGR19_001503"
     CDS             complement(367..1716)
                     /gene="gshA"
                     /locus_tag="WGR19_001503"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0799008.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            1976..3385
                     /gene="leuC"
                     /locus_tag="WGR19_001504"
     CDS             1976..3385
                     /gene="leuC"
                     /locus_tag="WGR19_001504"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0799009.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            3482..3736
                     /locus_tag="WGR19_001505"
     CDS             3482..3736
                     /locus_tag="WGR19_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799010.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            3798..4439
                     /gene="leuD"
                     /locus_tag="WGR19_001506"
     CDS             3798..4439
                     /gene="leuD"
                     /locus_tag="WGR19_001506"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0799011.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            4614..4874
                     /gene="dcm"
                     /locus_tag="WGR19_001507"
     CDS             4614..4874
                     /gene="dcm"
                     /locus_tag="WGR19_001507"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0799012.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            4920..5435
                     /gene="dcm"
                     /locus_tag="WGR19_001508"
     CDS             4920..5435
                     /gene="dcm"
                     /locus_tag="WGR19_001508"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0799013.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKNGLQKSA"
     gene            5450..5884
                     /locus_tag="WGR19_001509"
     CDS             5450..5884
                     /locus_tag="WGR19_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0799014.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            5900..6061
                     /locus_tag="WGR19_001510"
     CDS             5900..6061
                     /locus_tag="WGR19_001510"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0799015.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            6062..6631
                     /locus_tag="WGR19_001511"
     CDS             6062..6631
                     /locus_tag="WGR19_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0799016.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNTGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            6665..7735
                     /gene="leuB"
                     /locus_tag="WGR19_001512"
     CDS             6665..7735
                     /gene="leuB"
                     /locus_tag="WGR19_001512"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0799017.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            7898..8461
                     /locus_tag="WGR19_001513"
     CDS             7898..8461
                     /locus_tag="WGR19_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0799018.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         2385 a         2331 c         1972 g         1933 t
ORIGIN      
        1 actgtgtgaa acttgccgtc cccatccctt tgtatgccga cagtttgccg acctccgcgc
       61 tcatcagcct ccccaaactg ccgaactcct gcagcaaata acgcgccaaa tcgaccgcac
      121 tcattccgcg cgtgccgacg cgcagcagga ttgccaaaag ttcggcatca ctcaacgccg
      181 ccgccccgcg ttccaacagc ttttccctgg gcctttcccc ctccggccat tgcttgatgc
      241 tcatgttttc ccttttttaa atagtctgag atgccgtctg gaacaaatgt tcccacacgc
      301 caaatatccg aaatgccgtc tgaaccggat ttccgcttca gacggcatct gtatttcaaa
      361 acagtcttac gccgtttctt ccaactcgat ggacgccgcc agaagcgaca ggcgtgccat
      421 tacgccgtaa acgtagaggc ggttgtgttc gttgtccacg tcgcagtctt tttcagagcg
      481 cggcataccc agcgagtccc attgttcgaa tacgcgcttg caggcttcgg gcgcttcttg
      541 ggaattatcg ccgttaccgg taggaatgct gttagacagc ggaacaaaca ccataccgcc
      601 ggcgttgagg ttttcgtccg caccgcgccc ttcgtgtacg cggaaaaagc cgccgatgac
      661 gaagcggtcc atcatataca cgacgggttc gcacaccgcg ccgtttaagg tttcataagt
      721 ataaatccct tcttggacaa tcacttcgct gacttccaag ccttctttga ctttcgccat
      781 tttattgcgg tttttacggt tcaatccgcg cacttcgtcg gcggatttga cgctcatcac
      841 gcccatgccg taagtgccgg catcggcttt gacgatgacg aaaggtttgt cggtaatgcc
      901 cgattcgtcg tatttggctt gaattttcgc cagcacacgt tctaccgctt ccgccaacgc
      961 gtcttcgcct tcacgccctt ggaagtccaa accgccgatt ttttcaaaat aagggttaat
     1021 ttgccattcg tcgatgccga ttaacttggc aaattcggcg gtaacttggt tgtacgcgcc
     1081 gaaatgattt gttttgcggc gcgtcgtcca accgccgtgc aacggcggca aaacggtttg
     1141 gctgatgcct ttgaggatgt cgggaatgcc ggcagacaaa tcgttgttca acaaaaccac
     1201 gcaaggcgaa aagccgtctg caagatggac gcggtcgcgg gtacgcaata aaggttccaa
     1261 caggattttg tcgcccaatg cagtttcaaa ttcggtcggt tcggttactt ccggattcag
     1321 gctgcccaag cgcacttcat accctgccga acgcaaaatc tcgccgaggg cgtaaatgtt
     1381 ttgcaggtaa aacgtattgc gcgtgtggtt ttcgggaata atcaatacgg atttcgccgt
     1441 ttcgcaggcg cgttgcaccg catcttgcgc ggcaaccgcc gccagcggga taaagttggg
     1501 attcagatta ttgaagccgc cggggaacaa attcatatcg atagacgaaa ttttgtaacc
     1561 ggcattgcgt atatcgaccg aaccgtaaaa cggcgggcgg tgtacgctcc actgcgtacg
     1621 gaaccacgcc tcgattttgg cgtgattgga caggattttt gcctcaaacg cctgaagttg
     1681 cgccgaatgt tcgggcgaca tcaccggtaa tttcatcttt cgtctgcctc tgtgtgtttt
     1741 ttgacaagat gcagatgata atgcctttga aagaatgacg acaagtattc tttcgtatcg
     1801 aatcatgctt tagatataac tgtttactga aatggacaat acgtctaatt ttttcgttaa
     1861 aattgtttgc aaaataacac cggctcaaaa attagacaaa atttgttgcg cggtataaag
     1921 aatacgtcta aaatccgccc aatccgcatt accatttatc caaaagaaca acatcatgac
     1981 agcacaaacc ctctacgaca aactttggaa cagccacgtc gtccgcgaag aagaagacgg
     2041 caccgtcctg ctctacatcg accgccattt ggtgcacgaa gttaccagcc ctcaggcatt
     2101 tgaaggcttg aaaatggcgg ggcgcaagct gtggcgcatc gacagcgtcg tctccaccgc
     2161 cgaccacaac accccgaccg gcgattggga caaaggcatc caagacccga tttccaagct
     2221 gcaagtcgat actttggaca aaaacattaa agagtttggc gcactcgcct attttccgtt
     2281 tatggacaaa ggtcagggca tcgtacacgt tatgggcccc gaacaaggcg cgaccctgcc
     2341 cggtatgacc gtcgtctgcg gcgactcgca cacttccacc cacggcgcat tcggcgcact
     2401 ggcgcacggc atcggcactt ccgaagtcga gcacacgatg gcgacccagt gcattaccgc
     2461 gaaaaaatcc aaatccatgc tgattgccgt tgacggcaaa ttaaaagcgg gcgttaccgc
     2521 caaagacgtg gcactttaca tcatcgggca aatcggcacg gcgggcggta caggctacgc
     2581 catcgagttt ggcggcgaag ccatccgcag cctttctatg gaaggccgca tgactttatg
     2641 caatatggcg attgaggcag gcgcgcgctc aggcatggtt gccgtcgacc aaaccaccat
     2701 cgactatgta aaagataaac ccttcgcacc cgaaggcgaa gcgtgggaca aagctgtcga
     2761 atactggcgc acgctggtgt ctgacgaagg tgcggtattc gacaaagaat accgtttcaa
     2821 cgccgaagac atcgaaccgc aagtcacttg gggtacctcg cctgaaatgg ttttagacat
     2881 cagcagcaaa gtgccgaatc ctgccgaaga aaccgatccg gtcaaacgca gcggtatgga
     2941 acgcgccctt gaatacatgg gcttggaagc cggtacgcca ttaaacgaaa tccccgtcga
     3001 catcgtattc atcggctctt gcaccaacag ccgcatcgaa gacttgcgcg aagccgccgc
     3061 catcgccaaa gaccgcaaaa aagccgccaa cgtacagcgc gtgttaatcg tccccggctc
     3121 cggtttggtt aaagaacaag ccgaaaaaga aggcttggac aaaattttca tcgaagccgg
     3181 ttttgaatgg cgcgaaccgg gctgttcgat gtgtctcgcc atgaacgccg accgcctgac
     3241 cccggggcaa cgctgcgcct ccacctccaa ccgtaacttt gaaggccgtc aaggcaacgg
     3301 cggacgtacc cacctcgtca gccccgctat ggcagcagca gccgcagtga ccggccgctt
     3361 taccgacatc cgcatgatgg cgtaagtatt ttcaggcggt ctttaagaat ttgacgcggc
     3421 cgtctgaaat atgtcccgta aggcagaaac acctttaata ttcaaaccaa ggagtttaac
     3481 catgaacaaa cttttcatta ccgccctgtc cgccctcgcc ttgtccgcct gcgccggcac
     3541 ttgggagggc gcgaaacaag acaccgcccg caaccttgac aaaacacagg ccgccgccga
     3601 acgcgccgcc gaacaaacag gcaacgccgt cgaaaaaggc tgggacaaaa ccaaagaagc
     3661 cgtcaaaaaa ggcggcaatg ccgtcggacg cggcatttcc catctcggcg gaaaaatcga
     3721 aaacgccacc gaataaccga ttcgcgatgc cgtctgaaac cgcttcagac ggcatcgtca
     3781 gcacaagacg caaacccatg aaagccttta ccaaaattac cgccatcgtc gccccgctcg
     3841 accgcagcaa cgtcgatacc gacgccatca tccccaaaca atttttaaaa tccatcaaac
     3901 gcagcggctt cggccccaat gcctttgacg aatggcgtta cctcgaccac ggcgaaccag
     3961 gcatggacaa cagcaaacgc ccgttgaacc ctgatttctc gctgaaccag ccgcgttacc
     4021 aaggcgcgca aatcctgttg acgcgtaaaa acttcggttg cggctcttca cgcgaacacg
     4081 ccccttgggc attggacgac tacggcttcc gcgccgtcat cgcccccagc ttcgccgaca
     4141 tcttcttcaa caactgctac aaaaacggcc ttttacctat cgtgttgacc gaagaacagg
     4201 ttgaccggct tttcaaagaa gttgaagcca acgaaggcta tcagctctcc atcgaccttg
     4261 ccgaacaaac cctgaccacc cctggcggcg aaacattcac attcgacatt accgaacacc
     4321 gcaaacactg cctcttaaac ggcttggacg aaatcggact gaccctgcaa catgccgatg
     4381 aaattcacgc ctttgaagaa aaacgccgcc aaagccagcc ttggctgttt aacggttaaa
     4441 acctatgccg tctgaaggcc gttcagacgg cattcctgat aacatataat tgttgcggct
     4501 aatgtaaaca gaggggcgat aaattgcttt catcttttaa gcgggcttag cgacactcta
     4561 aattaatgca ataatgcaac tctccatttc aaataaattt ttagattttt aatatgcaac
     4621 aaattaaatt tattgactta ttttccgaaa tgagtggcat caggaaagga ttcgaacaag
     4681 cctgtcggaa acagtcggtt gcttgcgaat gtgtttttac ttcagaaatc aaaccggcag
     4741 ctttggaagt attgaagcaa aactaccctg atgaagtgcc gtatggagat ataacgaaaa
     4801 ttgaaacagg ggatattccc gattttgaca tcctgttggc aggcttccct tgtcaggctt
     4861 tttctttcgc ctgaaaaaga ttaggctttg aagatacacg gggaacgctt ttctttgatg
     4921 tggcaaggat tttaaaggca aaaaaaccaa aaggttttat tttggaaaat gtggaaggat
     4981 tggtgacaca cgatagaaaa gattcgacac aaaaaatagg gcgcaccctg accgttattt
     5041 tggaaacctt ggaagcattg ggctactatg tttcttggaa agttttaaat gcaaaagatt
     5101 tcggcattcc ccaaaacagg aagcgcattt atctgacagg cagtttgaaa tccaaacccg
     5161 acttatcttt cgaaacaagc ccaagtccga aattaaaaaa tattttggaa tcgggactgc
     5221 ctactgaaag cagccctttc atcaagaaat tgctaaaaaa atttcccccg tccgaactgt
     5281 acggaaaatc agtaaaagac aaacggggag ggaaaaacaa tattcacagt tgggatattg
     5341 aattaaaagg cgcagtaacc gaagaagaga agcaattgtt aaatatcctt ctaaaagaac
     5401 ggaggaaaaa aaatgggctt cagaaatcgg catagattgg atggatggga tgcctttgac
     5461 aaaagcgcaa atttcaactt tctataaaca ccccgatctc caaaatattt tggacagcct
     5521 gacagataaa ggctatttgg ttttagagca tccgaaacaa aaaattggcg ggcaaagaat
     5581 caaagatgaa tctctgccca aaggctacaa tattgtttca ggtaaaaaat cttttgaaat
     5641 caataaaata ttagatccaa acgatgttgc gccaaccttg gttgcaatgg atatggagca
     5701 ccttttcgtc gttgacaacg gcggtttgcg tacacttacc ggaaaagaag ggttgcgctt
     5761 attcggctat ccggacgatt attcgtttga tattcccaaa aaagacagat gcgatttatt
     5821 gggtaatacc gttgccgtcc ctgtgattaa ggcggtatct gaaagacttc tgcatacttt
     5881 ataaaccgac agggataaaa tgataaaact tactgcacaa caaatatttg ataagttgct
     5941 ggatgaagaa aaaatcttat cggccaatgg tcaaattaga tttttcttgg gcgatgtgga
     6001 tattatcgtc aaacaaaaag atgttgtcgg caacatcatt caggaatggc tcggcggatg
     6061 attgagaaaa agggaaattg aatttgatgt ttcaaccaat acccaaatgc ctcccgattt
     6121 ctttttaaat aaaaaagacc gcagcagaga attgttggaa gtaaaggcgt tcaaccgaaa
     6181 taccggcccg ggttttgata ttgcagattt taaaatgtat tctgatgaaa tcattcataa
     6241 gccctatatg ctggatgtag actatttaat attcggttac gatatggacg acaacggcaa
     6301 tgtaaccatc aaggatttat ggcttaaaaa agtatggcaa attacgagaa gcatggatgg
     6361 gtgggcaatt aatcttcaag tcaaaaaagg cgtggtgcat aaaatccgcc cgggtgtttg
     6421 gtacagcata aataaaaaga atatgcctat gtttgaatgc ttggaagatt ttgtttccgc
     6481 aattgaagaa accgtttatc aaaacccggc aacacggcat aacgcatcct tatggaaaaa
     6541 gaaatttgag gaagcctata aaaaacacta caaccgatcg atttccatac ctcgttggca
     6601 cgaaattgca cacaaataca aaaagaaata acccttcaac catcaaccca taaggaaact
     6661 tgaaatgacc aaacatatcg ccatcctccg aggtgacggc atcggtcccg aaatcgtcgc
     6721 cgaaaccgtc cgcgtactcg acaaactcat cgcccaaggt ttggatgccg gctacgaata
     6781 cgccccattg ggcggcgaag cctatgacga atacggtcat ccttatcccg aatttacgca
     6841 aaacctctgc cgcaaagccg atgccgtcct gcttggtgca gtcggttctc ctcaatacga
     6901 caacctcgac cgtccgttgc gccctgagcg cggcctgttg gcaatccgta aggatttgaa
     6961 cctgtttgcc aatttgcgtc ctgctgtttt atatcccgaa ttagccaacg cctccacgct
     7021 gaagcctgaa atcgttgccg gtttggatat tttgattgtg cgcgaactca ccggcgacat
     7081 ctatttcggc gaaccgcgcg gcatccgcgt tctggaaaac ggcgaacgcg aaggctacaa
     7141 caccatgaaa tacagcgaaa gcgaaatccg ccgcatcgcc cacgtcgctt tccaatccgc
     7201 ccaaaaacgc agcaaaaaag tctgctccgt aggcaaagcc aacgttttgg aaaccaccga
     7261 actgtggcgc gaaatctttg aagaaatcgg caaacaatat cccgatgtcg aactttccca
     7321 tatgtatgtg gacaacgccg ctatgcagct cgtgcgcgcg cccaaacagt ttgacgtgat
     7381 tgccaccggc aacatcttcg gcgacatcct ctccgacgaa gcctccatgc tgaccggctc
     7441 catcggtatg ctgccttccg cttctttgga cgaaaacggc aaaggcctgt acgaaccgtc
     7501 ccacggctcc gctcccgaca tcgccggaca aaacaaagcc aacccgctgg ccaccatcct
     7561 ctcgctcgca atgttgctgc gttacagcct gaacgacgaa gcgcgcgcgc aacaagttga
     7621 aaacgccgtc caaaaagtgc tgcaacaagg cttgcgtacc agcgacattt acgaagaagg
     7681 cacaaaactc gtttcctgct ccgaaatggg cgatgcggta ctcgccgcct tgtaaaaaaa
     7741 caaagcgcgg atcacaccgc gctttttcat ctgccctttt ccgctcgccc cgttcgtcaa
     7801 ccgcctgcaa agccaccgtt taccggcagg aaatgccata aatccccgcc tatgttagga
     7861 taaacgcctt tactggctca cacaaaggat tatcatcatg aaaaaaatca tcttcgccgc
     7921 actcgcagcg gcagccgttg gcactgcctc cgccgccacc tacaaagtgg acgaatatca
     7981 cgccaacgcc cgtttcgcca tcgaccattt caacaccagc accaacgtcg gcggttttta
     8041 cggtctgacc ggttccgtcg agttcgacca agcaaaacgc gacggtaaaa tcgacatcac
     8101 catccccgtt gccaacctgc aaagcggttc gcaacacttt accgaccacc tgaaatcagc
     8161 cgacatcttc gatgccgccc aatatccgga catccgcttt gtttccacca aattcaactt
     8221 caacggcaaa aaactggttt ccgttgacgg caacctgacc atgcacggca aaaccgcccc
     8281 cgtcaaactc aaagccgaaa aattcaactg ctaccaaagc ccgatgttga aaaccgaagt
     8341 ttgcggcggc gacttcagca ccaccatcga ccgcaccaaa tggggcgtgg actacctcgt
     8401 taacgttggt atgaccaaaa gcgtccgcat cgacatccaa atcgaggcag ccaaacaata
     8461 aatcttgatg cgaatcagca caaatgccgt ctgaacgtcc ttcaaacggc atttgtgccg
     8521 tcttcatcaa accgccccgc caacggtaag ccgatatggc ggagcacaat ccgccgaaag
     8581 atatttcagc ggcaggcagg gcttgccgga aagtccggaa a
//
LOCUS       DAWFJW010000080         8569 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFJW010000080 DAWFJW010000000
VERSION     DAWFJW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.080"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            155..1339
                     /gene="coaBC"
                     /locus_tag="WGR19_001514"
     CDS             155..1339
                     /gene="coaBC"
                     /locus_tag="WGR19_001514"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0799019.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1406..3562)
                     /locus_tag="WGR19_001515"
     CDS             complement(1406..3562)
                     /locus_tag="WGR19_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0799020.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMGIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3654..3860)
                     /locus_tag="WGR19_001516"
     CDS             complement(3654..3860)
                     /locus_tag="WGR19_001516"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0799021.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3919..4536)
                     /gene="gmk"
                     /locus_tag="WGR19_001517"
     CDS             complement(3919..4536)
                     /gene="gmk"
                     /locus_tag="WGR19_001517"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0799022.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4664..5263
                     /locus_tag="WGR19_001518"
     CDS             4664..5263
                     /locus_tag="WGR19_001518"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0799023.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5309..6097)
                     /locus_tag="WGR19_001519"
     CDS             complement(5309..6097)
                     /locus_tag="WGR19_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0799024.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6422..7777
                     /gene="yegQ"
                     /locus_tag="WGR19_001520"
     CDS             6422..7777
                     /gene="yegQ"
                     /locus_tag="WGR19_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0799025.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLNVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPGGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7911..8387
                     /locus_tag="WGR19_001521"
     CDS             7911..8387
                     /locus_tag="WGR19_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0799026.1"
                     /translation="MLAVCQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIAGGVLESGIPVSFGGGGVPVANGQGLVTRQAANGVGKSDSEACERA
                     LINAARKFQQTAGKLGGRGVTVFPSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT         2143 a         2344 c         2050 g         2032 t
ORIGIN      
        1 ttcctccccc tcctctgact ggtgcgcctt tgtgaatatg ccgtctgaaa cttggggctt
       61 cagacggcat tttgtatcca aaacggtaac taatgtatcc gtactttgtt atagaatggc
      121 tgctgttttt tcttcgtaat tagaaattgt caaaatgggc aaacatattc ttttaggtgt
      181 aacgggcagt attgcggcgt ataagtcttg cgagttggtt cggctgctga aaaaacaggg
      241 gcattcggtt acggtggtta tgagccgctc ggcaactgaa tttgtttctc cgctgacttt
      301 tcaggcttta agcggcaatc ctgtcctgac cgacacgcac ggcggcaacg gttcaaacgg
      361 tatggaacat atcaacctga cccggaatgc ggatgttttt ctgattgcgc cggcaagtat
      421 gaataccgtg gcaaaaatct gtaacggcgt ggcagataac ctactgacca atctggcagc
      481 cgcacggaaa tgtcctcttg ccatcgctcc cgcgatgaat gtggaaatgt ggctgaatcc
      541 tgccaaccaa cggaatatcg cacaactggt ttcagacggc attactgtct atatgccggg
      601 cttgggcgaa caggcttgcg gagaaaatgg tatgggaagg atgccggaac ctgccgaatt
      661 gctggatctg cttccggatt tatggacacc gaaaatttta aagggcaaaa aagtcttgat
      721 taccgcaggt gcgacatttg aagccattga ccctgtccga ggcatcacaa atatctccag
      781 cgggaaaatg ggcgtggctt tggcgcgggc gtgccgtgcc gccggtgcgg aagtcagcct
      841 gattcacgga cagcttcaaa ccgcgctgcc tttcggcata tccgatacgg ttcaagccgt
      901 cagtgccgaa aatatgcatc gcgcagtgca tcgtttaatc gacaaacaag atgcttttat
      961 ttctgttgcc gccgtctcag actataaggt taaaaacagg agtacgcaaa aattcaaaaa
     1021 agataaaaat gccaaacggc tatccatcga attggatgag aaccccgata ttttggcttc
     1081 tattgcctca ttgccgaacc cgccgttctg catcggtttt gccgctgaaa cggagaatgt
     1141 aatggcatat gcacgagaaa aacgtattaa gaaaaaaata ccggtaatag ttgccaatga
     1201 tgtttcaatc gcgatgggca aaccgaccaa ccggattacc attatcgggg acgacgggga
     1261 actatctttt cccgaaacaa gtaaagatga agcggcgatg cagattgttg aaaggcttgc
     1321 cgtatatttg aacaaataag caattgaacg gataaaccat aaagcgggtt gcctgttaat
     1381 caaaaggcaa cccgctttac ctgcttcaac ttctgatgac tttgcggata tatggaatac
     1441 tatgcagatt ttgaataatc tgattcaatt gattcagatt cttgactttc aataagaatt
     1501 tgaattcgac aaaaccttcc gttcccgact gggatttaga cggtgtttcg accgactcaa
     1561 tgtccgcacc ggaatcggaa atcgcttgcg ccattaatgc caacaggccg tggctgtctt
     1621 ccgattggac ttgaagcccg acacggtagt tctgcccgtt catattttcc cagtctgcat
     1681 ccagctgctg ttcgggatcg gacttcaaca acgtcgggca ggtatctctg tggataatca
     1741 tgccttttcc cttaaccaac agcaaacgga tggaatcacc aggaacaggg tggcagcact
     1801 ctgcaaaatg aatatgcccg ctttcctgac catcaacttt aatggaactg agcctgacct
     1861 cgctgccgaa atgctcccct gccaactcgg caatgtgcat ggcgacataa acaggcaggg
     1921 tatgccccat accgacgttg tacagcactt cctcaaatga agtctgtttg tcgttgagat
     1981 cggcaaggta tttttctttg atgccgtctg aaagcaggac atctttgggc agcaaactgg
     2041 acagggcttt ttgtaagagg ctctctccca aaacgaccgc atcgtgccgg ttaaggtttt
     2101 taatatattg gcgtatggca ctgcgcgccc tgcctgacac ggcgaaattc aaccatgcag
     2161 gattgggttt ggcatgttcg gatgtaataa tttcgacgga atcgcctgtt ttgagcttcg
     2221 tacgcaacgg catcatgata ttgttgatac gtgcggcaac ggttttgtgc ccgatatcgg
     2281 tatgcaccgc ataagcaaaa tcgacaggcg ttgccccttt gggcaaagtt aggatttttc
     2341 cttttggcgt aaggatgtag atttcgttcg gaaacaaatc gactttgacg tgttcgagaa
     2401 actcaatggc attggcactg cttgcctgca aatctaagat atttttcagc caccggtttg
     2461 tgtgaagcac cgcctgatcg accgtcttag aatatgattt atagctccaa tgtccggcga
     2521 ttccaccttc ggcaacagca tccatttcct tggtacgtat ctgaacttca atcggcaagc
     2581 cgtaaggacc gaccaaagtc gtatgcagac tttgataccc gttgcttttc ggaatggcga
     2641 tatagtcttt gaaccgcccg ggcttgggct gatagagggt gtgcaatgcg ccgagtgcgg
     2701 cataacaggc tggaatgctg ttgacaatga cgcggaaacc gtaaatatcc ataacctcgg
     2761 caaagcgcag ctttttcgcc atcattttct gatggatgcc gtacaggttt ttttccctgc
     2821 ctttgatttt ggcctctata ttcgcgccta ccagccgctg gccgaatgcg cgcaagactt
     2881 tgccgacaac gtcctgccgg ttcttccggc tcttgtccat cgcttttttt aaagtctcgt
     2941 agcggttggg atgcaggttt tggaacgata aatcctgaag ctcttgatat gcgttattca
     3001 aacctatacg gttagcaatc tgtgcataga tttcaagggt ttcccttgca atccggcggc
     3061 gtttgtccgg gcgcatcgaa ccgagcgtcc gcatattgtg caggcggtcg gcaagtttga
     3121 cgacgatgac gcgcacatct ttggtcatcg ccaaaatcag tttgcggaaa ctctccgcct
     3181 gatgttccgc atgatcttca aatttgagtt tctcgagttt ggacagcccg tccaccatct
     3241 cggcaatcgt attgccgaac accgccgcca tttccccttt tgtcacgccc gtatcttcca
     3301 atacgtcgtg catcacgcct gcacaaagac cctgtatgcc catatgccaa agggcgagct
     3361 gcgtcgcaac ggcaatcgga tgcgtgatgt agggctcccc gcttttgcgg gtttgcccgt
     3421 cgtgggcacg aaacgcatag gcgacagctt tttcaagctc cgcctgttcc tcgggcttga
     3481 ggtaggaggc ggtatggaaa agcagggcac gcgcttcggc ggtcaggggg tcgtaagggg
     3541 cggaaggttg gggggcgggc atttcagacg gctttcggta tgtatgtgtt tttcatttca
     3601 aaccgtcgga ctgcacggcg gcaaagtgtt ccggcgtgcg tttccggcag aatttattta
     3661 ttgcgcgtca acagttctgt accgatatgt ccggcggcga tttcccttaa ggcggtaacg
     3721 gtcggtttgt tattgcggac atcgtccaca agcggcgtgt tgccgttctc aagctggcgg
     3781 gcgcgacggg ccgctaccaa tgtcaggtca aaatggttgg aaatttttcc ggtacagtct
     3841 tcggtggtaa tacgtgccat attatttgct ttctttcaaa aatatttaaa ataggaaact
     3901 gggtattttc gcggtttttt aggaattttc caacaaatct gcaataaatc ccagttgccg
     3961 cgaccttttc agacggcagg cattcacaat atggcgcaaa tcctcctccg cccgcgccaa
     4021 gtcgtcattg accacgacaa agtcgaacaa tacggactgt tcgatttcat gccttgcctt
     4081 cgcaagcctc ctttggataa cttcctgact gtccgtcccg cgtccgttga gacgcgcggc
     4141 aagcacatcg aaagacggcg gcaggataaa gatgccgaca gcttcaggca gcgcatctcg
     4201 aacctgcgcc gcgccctgaa cgtcgatttc caaaatcacg tcataacctg ccgccgccaa
     4261 cgcattcacg ccctccgcgc ttgtgccgta atagttgccg aatacgtctg cgtattccaa
     4321 aaaagcctcc tgtgcgataa gcgactcaaa ctcttctttg gaaacaaagt gataatgtac
     4381 gccgtttgct tcgccttcac gcggcgggcg cgtcgtgtgc gacacggaaa cgcgcaaacc
     4441 gttatggttt gccaacagcc gcgacaccag cgtggttttg cccgtgccgg aagcggccga
     4501 aatgataaag atgttgcctt ttcgataagc ggacatattt tttacctgta tattttccag
     4561 ccgattgtat cacaatggac acccagtttc ctatttgccg atgcccatat tttgccgcta
     4621 ttgttttgat tgatttggca agcggcaggc tgacggctac aatatggcgt taaaaacatc
     4681 aaacttggaa cacgcaatgc tggttcatcc cgaagctatg agtgtcggcg cgcttgccga
     4741 caaaatccgc aaaatcgaaa actggccgca aaaaggcatc ttattccacg acatcacgcc
     4801 cgtcctgcaa agtgcggaat acttccgcct tttagttgat ttattggttt accgctatat
     4861 ggatcagaaa atcgacatcg ttgccggttt ggacgcgcgc ggcttcatta tcggcgcggc
     4921 actcgcctac catctcaacg tcggttttgt ccccatccgc aaaaaaggca agctgccttt
     4981 tgaaaccgta tcgcaaagct acgcgctcga atacggggaa gctgcggtgg aaatccacac
     5041 cgatgccgtc aaacccggtt cgcgcgtgct gctggtcgat gatttggttg ccacgggcgg
     5101 cacaatgctt gccgggctgg aactgatccg caaactcggc ggagaaattg tcgaagccgc
     5161 cgccattttg gaatttaccg accttcaagg cggcaagaat atccgtgcaa gcggcgcgcc
     5221 cttatttacc ctgcttcaaa acgaaggctg tatgaagggc tgaaaaccga ccctgccgtc
     5281 tgaaaccggc agggttgtta tgatgcgttc aaatcacgcc caaatcttgc aagcccctca
     5341 acacgccgtc ttcatcaacg ccggggcaaa catatttcgc cgcctctttc actgcctgtt
     5401 ccccgttacc cattgccaca ccgaacccga cttcagacag catttccaca tcgttcaaac
     5461 cgtcgccgaa cgccatcacg tccgccattt ccaaacccaa tgcttcaacc acgcttctga
     5521 tgccgtctgt tttcgacatt cccgcaggca gcagatcgac cgcttcctcg tgccagcgca
     5581 ccgttttcaa gccttcccgt tccacaatat ccgaccaggg cggcatttcg ttttcctccg
     5641 caaacaccag catctgatac accggtttgc ttgaaaaata atccttatcg gcaaaaaaat
     5701 cgctggcgat atgctgcaag gcgcggcaca cgcattccga cagcgcggac acggcgatcc
     5761 cctctccgcc gacaaacgca taatccatac ccaagccatc caaatgcgcg caaaccctgc
     5821 ccatcaaacc ggcatccatc ggtacttcgc gcacagtttt tccgtgcagc agcgcaaact
     5881 gtccgtttat cgttaccacg gcatccatac ccgtttccgc catcatgtcc ctgacctttt
     5941 cgggaatcgt cgccaaagac cgccccgttg ccaacgccgt caatatacct ttgccgcgca
     6001 aagccgccac cgccgttttc acggaaggac gcaaggtatc cgtatatttg cggtacagcg
     6061 tatcgtcaat atcgaaaaac acgattttag gattcatcac attctctctc gcattcaaac
     6121 tgccgcattg tatcccaatc aggcaaatac ttgataaatc cttataaatt tcccggcaaa
     6181 attgaccgcg aacacgaaaa ggcggataat ccgcccatcc tcaaaccctt ttcagacggc
     6241 atttgcagca atgccgtctg aaacattttt acaaagcaca caaatcatgt ttcaacacac
     6301 aggacgacac ataaagcgtc gccctatatg ttgccctgat tcggaagggg ttacgcccct
     6361 cccaaataaa gtctgattct accgccctaa agggcggggt ttcaaccgaa aaggaaacac
     6421 gatgaaagca cccgaactct tattgcccgc cggcggattg gaaagaatgc gcgccgccta
     6481 cgattacggc gcagatgccg tttacgccgg cagcccgcgt tactccctgc gcgcccgcaa
     6541 caacgaattt gccaaactca acgtccttga gcaaggcatt aaagaagcgc acgagcgcaa
     6601 caaaaaattc ttccttaccg tcaacaccct gccgcacaac tccaaactca aaacctttgt
     6661 ttccgatatg gagccgctga ttgcgatgaa gcccgacgcg ctgattatgg cggatccggg
     6721 tttgatcatg accgtgcgcg aaaaatggcc ggagatgccg attcacctat ccgtacaggc
     6781 aaacaccacc aactactggg gcgtgaaatt ctggcagaac atcggtgtcg aacgcattat
     6841 tctgtcgcgc gaattgagca tggaagaaat cgctgaaatc cgccaagaat gccccgatat
     6901 cgaactcgaa gtcttcattc acggcgcatt gtgcatcgcc tattcaggcc gctgtctgct
     6961 ctccggctac ttcaaccacc gcgaccccaa ccaaggcacc tgcaccaact cctgccgttg
     7021 ggattacaag gttcacaatg ccactgaaag cgatgcgggc gatgcccagc ttctgcaagg
     7081 tttcaacttt gaaaaagccc aagaagaagc caaccaaaac tttgaaggca tcaacggtca
     7141 aaaacgccat ccctacgccg acaaggtttt cctgattgaa gaatccaacc gtccgggcga
     7201 gatgatgccg attatggaag acgaacacgg cacctacatc atgaactcca aagaccttcg
     7261 tggcattgaa gtggttgaga aactcgccaa aatcggcgtg gacagcctca aagtcgaagg
     7321 ccgcaccaag tcgctctatt atgtcgcacg cgtcgcccaa tcctaccgca aagcgattga
     7381 cgatgccgtc gcaggccgtc cgtttgatta cagcctgttg agcgaactcg aaggccttgc
     7441 caaccgcggc tacaccagcg gcttcctcga acgccaccac actcaggatt atcaaaacta
     7501 cctgaccggc cattccaccg ccaaacaaag ccaatacgtc ggacacgtta ccgaaatcga
     7561 caaaaacggc tgggcgacca tcgaagttaa aaaccgcttt gccgtcggcg attcgctcga
     7621 aatcatccac ccgggcggca accaaaccat caaattggaa caaatggccc gaaaaggcca
     7681 gcctgtcgat gtcgcacccg gcaacggcat tcaggtcaaa atccccgata tgcagggcaa
     7741 agaaaaagcc ctcatcgcac gcgtgttgaa cccctgagcc acaatgccgt ctgaaccccg
     7801 ttttcagacg gcattttgtt gaatcttacg gtacaatggc aatgatttcc caccgcaagc
     7861 aaggatatcc gacatgaaaa aaaatcctca tactctccgc cgccgccctg atgctggcag
     7921 tctgccaatc cggcagcgca cctaaaaacg cgcacacggc atcccccggc acaccggccg
     7981 gagaatcatg caaagtcatc cggggcgaca ccgcaggaag cggcaaaaac atcatttacc
     8041 gttgctgcaa cggtcaggcg gcaataaatg cagccattgc aggcggtgta ttggaatccg
     8101 ggattcccgt cagcttcggc ggcggcggcg taccggttgc aaacggtcag ggcttggtta
     8161 cacggcaagc agcaaacggc gtaggcaaat ccgactccga agcctgcgaa cgcgccctaa
     8221 tcaatgccgc ccgcaaattc cagcagaccg ccggcaaact cggcggacgc ggcgttaccg
     8281 tcttccccag ctactttgac aagcagccgc tgcaaggcgg acaatacgac tgtcaggcag
     8341 gctcgttcca cgtccgcgtg gtcatgcgcg ccaatgtcgt ccgttaacat atcggcaatc
     8401 aaaaaattag gctgtgtttt agtaatctgt tgatttcaat tatttgcaaa ggaaaagaca
     8461 attatttttc ggttaggaag agacctatcc tattgaatac atttaagtta aatacgtcta
     8521 tcaacactat attatagtgg attaacaaaa atcaggacaa ggcgacgaa
//
LOCUS       DAWFJW010000081         8395 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFJW010000081 DAWFJW010000000
VERSION     DAWFJW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.081"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..141
                     /locus_tag="WGR19_001522"
     CDS             <1..141
                     /locus_tag="WGR19_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEZ0799027.1"
                     /translation="DYYNPKAEHSLIWNDPTVGIDWPLQGEPNLSPKDLAGKVLAEAV
                     TF"
     gene            165..2279
                     /locus_tag="WGR19_001523"
     CDS             165..2279
                     /locus_tag="WGR19_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0799028.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPYLSTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADLWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQTDILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            2416..3675
                     /locus_tag="WGR19_001524"
     CDS             2416..3675
                     /locus_tag="WGR19_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ0799029.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELQGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPTT"
     gene            4045..5451
                     /locus_tag="WGR19_001525"
     CDS             4045..5451
                     /locus_tag="WGR19_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0799030.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGITAACWQLGQIYFYGTGVSPNHAQAEQYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETTYRLAEAQTHAIGCHVDYNAARKNYMDAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5669..6883
                     /gene="gltS"
                     /locus_tag="WGR19_001526"
     CDS             5669..6883
                     /gene="gltS"
                     /locus_tag="WGR19_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0799031.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6982..7998
                     /locus_tag="WGR19_001527"
     CDS             6982..7998
                     /locus_tag="WGR19_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0799032.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVRKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDVGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            8009..8206
                     /locus_tag="WGR19_001528"
     CDS             8009..8206
                     /locus_tag="WGR19_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799033.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8224..8373
                     /locus_tag="WGR19_001529"
     CDS             8224..8373
                     /locus_tag="WGR19_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799034.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVTYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         2132 a         2453 c         1951 g         1859 t
ORIGIN      
        1 gactattaca accccaaagc cgaacactcg ctgatttgga atgatccgac cgtcggcatc
       61 gactggccgt tgcaaggcga gccgaacctg tcgcctaaag acttggcagg caaagtattg
      121 gccgaagcag tcacgtttta agactattca ggccgtctga aagaatgttc ctcttttcag
      181 acggcctaca atccattaat aacaataatc gacgaaaacg cattgtgaaa aacgcctaca
      241 tcccctctcg cggcatccgc aaaatcccct atctctccac tctattgcct gagtttcata
      301 tctgcaagga tgggaaagga gcagaggctg ttatcggctg gggtttgcgc ccgacgacac
      361 acaaagcgcg tgcttttgcc accgaacacc agcttccctt tattgctttg gaagacggct
      421 ttttacgctc gctcggactg ggcgtctccg gttatccccc ctactccatc gtctatgacg
      481 acatcggcat ctactacgac actacccgcc cttctcgttt ggaacaactg attcttgccg
      541 ccgataccat gccgtctgaa accttggctc aggcgcaaca gacgatggat ttcatcctgc
      601 aacaccacct gtccaaatac aaccacgcgc ccgaactttc agacgatcat cctttacgct
      661 ccccatccaa gcctgaaact gtcctcatta tcgaccaaac cttcggcgat atggccatcc
      721 aatatggcgg cgcagacgcc tctacgtttg aactgatgtt tcagacggcc ttaaatgaaa
      781 acccgcaagc cgatctttgg gtcaaaaccc accccgatgt tttgtgcggc aaaaaacaag
      841 gctatctgac ccagctggcg cagcaacacc gcgtccatct tttggctgaa gacattaatc
      901 cgatttcgct gctgcaagcg gtggacaaag tgtattgcgt gacctcgcaa atgggttttg
      961 aagccttgtt gtgcggcaag cctttggtaa cgtttggttt gccgtggtat gccggctggg
     1021 gtgtaagcga cgaccgccat cctgaaatca accgccttgt tcaaacccaa cgccgcgcca
     1081 cccgcaactt gctgcagctc ttcgccgcag cctatctgca atacagccgc tacctcaacc
     1141 ccaataccgg cgaagcaggc agcctctttg atgtcatcga ctatctggcg acggtcaaac
     1201 gtaaaaacga caaattgcgt ggcgagttat attgcgtcgg tatgtctttg tggaaacgcg
     1261 cggttgccaa accgttcttt aacgtaccct cttgccgtct gaaatttatc tcttccaccc
     1321 aaaaactggc aagggtcaaa ctgtccgacg atgcacgcat cctggcttgg ggcaacggca
     1381 aagaggccat cgtccgcttt gccgaacaac accacatccc cctgctgcgc atggaagacg
     1441 gctttatccg ctcggtcgga ctcggctcca acttagtgcc gccgctgtcg ctcgttaccg
     1501 acgatatgag catttatttc aatgccgaaa ccccgtcccg tcttgaatac atcctacaaa
     1561 accaaaactt cgacgatcaa gactttcaga cggccttgaa gctgcaaaaa atgctgaccg
     1621 aaaaccacat cagtaaatac aacgtcggca gctcagactt caccgccccg tcaaccgaca
     1681 aaaccgtgat cctcgttccc ggccaggttg aagatgatgc gtctatccgc tacggttcgc
     1741 cccaaatcta ccgcaatctg gatttgctcc gtaccgtacg cgaacgaaac cccaatgcct
     1801 atatcatcta caaaccgcat cccgatgtag tcagcggtaa ccgcatcggc catatttccc
     1861 ctgaagatgc tgcacgatat gccgaccaaa ccgccgagca aaccgacatc ctgacctgtc
     1921 tccaatacgc agacgaagta cataccatga cttcgctgac cggttttgaa gccttgttgc
     1981 gcggcaaaaa agtcagctgc tacggcctgc ctttttacgc aggctgggga cttacccaag
     2041 atctgctccc cattccgcgc cgtagccgca gacttgagct ttggcagctg attgccggca
     2101 cgctcataca ctatcccgac tatatccatc ccgaaaccca tcaggccatc aatgcggaaa
     2161 ccgctgccca aatcctgata cgacaaaaaa atatgcaaaa aaacaacaac ggattacatc
     2221 gcgggtactt tgccaaaaaa ttaggtaaaa tcaaacaact atatcgatct ttcaaataaa
     2281 taccatcaaa gttaacgatg cgtcataaac ttgcctctat tgcggcatca ttgcctttgc
     2341 atcgttaatt ctcttggcgt atgcttgaaa gttcaaccta aaactattac ataaaaaaca
     2401 aaaccacatt gcaacatgaa acagaccgtc ctcaaaaata acctgcaaaa cctgcttgaa
     2461 agcgcagaaa atatcctgct gcttcaaggc cctgtcggcg atttttttct gcgccttgcc
     2521 gactggctga ctgcaaacgg caaaaccgta cataaattca actttaatgc aggcgacgac
     2581 tatttttatc cgcccactca agcgcatacc gttgttttta acgacaacta cgatgccttt
     2641 cctgagtttt tgcaagaata catcactcaa catcacatcc aggccgttgt ctgctttggc
     2701 gacacacgcc cttatcacgt cattgcaaaa cgcattgcaa acgaaaacca agccagtttc
     2761 tgggcgtttg aagaaggcta tttccgcccc tactacatca ccttagaaaa agacggcgtc
     2821 aacgcatttt ccccgttgcc gcgccgtgcc gacttttttc ttgaacaatt ccctaagctt
     2881 gcccagcaag aatataaagc gccaacgccg gtacacggcg gttttacgcc catggcaaaa
     2941 aacgctatcc gttactatat cgagttgttc cgcaatccac gcaaataccc cgactacatc
     3001 caccaccgcg cacccaatgc cggccattac ctcaaaccgt ggtcgctctc catcctcaag
     3061 cgtttgaact actatattga agacatccaa atcgccaaac gtgtggaagc aggcaaatac
     3121 ggcaagtttt ttattgttcc cttacaggta ttcaacgaca gccaagtccg tatccattgc
     3181 gactttccca gcgtccgcag cttcctgctc catgttttga gttcatttgc cgagcacgcg
     3241 cctgccgata ccaacatcat catcaagcat catccgatgg accgcggttt tatcgactac
     3301 tggcgcgaca ttaaacgctt tatcaaagaa caccccgaac tccaaggccg tgtgatttat
     3361 gtccatgatg tccccctgcc cgttttcctg cgccacggtc tcggcatggt taccatcaac
     3421 agcaccagcg gcctgtccgg actgattcac aatatgccag ttaaggttct cggccgtgcc
     3481 tattatgata ttcccggtat tactgaccaa aataccttgg cagaattttg gaatcatccg
     3541 acaccgcctg acaaagagct gttccatgcc taccgaatgt accacctcaa cgtgacccaa
     3601 attaacggca acttctacag tcaggtcttt tttcctaaca aaaacacatc cgactcttcc
     3661 acaccaacaa cctaacttgg cgaaagaaaa gctcaggccg tctgaaatat ttttcagacg
     3721 gcctgaagca atcaatacct tagctactgc catgtaaata aaacacaaaa acctgcattt
     3781 atcattaaca ataaattaca aaaacagtat aatgaccgag ctgtcatgag cgcataccga
     3841 ctcaacctga gccctttgta acacacaaaa tatggatata cccccaggca aaacaatata
     3901 acaagccaaa catcctaaag ataagccggc aaggcaatac actctataaa actgtgccga
     3961 gcaaaatttt tacaaagccc tcaaccggta tcgccgccca tatgccgcag catccgtctt
     4021 ccactttata tccgcccgca aaccatgacc gccgctcctg atatcctcta ccggcaagcc
     4081 gccgcccttt tggaacaatc caataccgcc caagccctgc ccctgttgca acaggcggca
     4141 gagcaaggtt atgcggaagc tgctttcgta ttgggcaacc atctgctgca aaacggccaa
     4201 ccggagcagg cactttcatg gttggaagcc gccgcggccc aacgccatcc caaagcactc
     4261 ttctccctgc tgcaacaacg cgaacacaac ggcaccccga ccggacagct tctcaacgaa
     4321 tacgcctggc tgagtgagca aggtcacctc gaagcccaat taattctcat gcgttaccac
     4381 acgcagcgca acgatccaca atcgctctac tgggcggagc ttgccgctgc ccaatatgcc
     4441 gcacctgcgt attaccattt ggcacgccat catcaacgcc aaggcgacgt tgcgacagcc
     4501 atcgaacaat acgaaaaagc ggcagcactc ggcataactg ctgcctgctg gcaactcggt
     4561 caaatctact tctacggtac aggtgtcagc cccaaccacg cacaagccga acaatatctc
     4621 gaacaagcgg cgcaagccgg ccacatcgcc gcacaaaccc tgctggccga ccttcttgcc
     4681 gcccaacgca aacccgaagc cttagaatgg tatcgtcgtg ccgccaataa ggagcaagcg
     4741 gaagcacaag ccaagctggc ccaatacgcc ctgaccggcg aactttccga acgcgatccg
     4801 tttcaagcgg cacgatatgc caaagccgcc gctgagaaag accatcccga agccctgaaa
     4861 atcatgggcg acctctaccg ctacggcctc ggcatcaaag ccgacaacca tattgcgcat
     4921 gattactacc accgtgccgc cgcgctgggt tctgccgccg cagcacaaaa actcatcagc
     4981 gacgccgcgc tgtaccatcc gcaacaatac gaacaaatca aaactgccgc cctacaacag
     5041 caacaaaccg agaccacata ccgtttggcg gaagcacaaa cccatgccat cggttgccac
     5101 gtcgactata atgccgcgcg caaaaattac atggacgctg ccgggttcca ccataaaaac
     5161 gcggcggccg ccttaggccg catctaccat tacggcctcg gtacggcgca agatcctcgg
     5221 gcggctgcac actggtacgc cattgctgcc gaacaaaacc acccttccgc ccaataccac
     5281 ctcgcctgtt tttactatca cgggcaaggt gtcggctgtc atgttccgac cgcctgctac
     5341 tggctgcagg ccgccatcgg caacggccac acttcggccg aatcattaat atccctatta
     5401 gaacaatggc gacgcgaagc acaccatgcc atcggacaaa aggccgtctg aaaagattta
     5461 cactcgcatt ttttgacaat ctttaactat tcccctaata tttgccagtt atttttcacg
     5521 gacacgccat tgttttcatt tctttctgaa aacaccttgt ccgcgcatca ataccatgac
     5581 actcggcgga taacgccaag cgttgaaaca cactacatcc ggaacaaaaa cggatgctcg
     5641 gaaaaatatt tctaggaggt gaaacaacat ggaatgggaa ttcaacagtt attacacact
     5701 gattgccgcc acgctcgtgt tgctggttgg taaatttctg gttcaaaaaa tcaaattctt
     5761 acgagacttc aatatccccg agcccgtggc gggcgggctg attgccgcga ttatcctgtt
     5821 tgcgctgcac gaggcgtacg gcgtgagctt caaatttgag aaaccgctgc aaaatgcgtt
     5881 tatgctgatt ttcttcacgt ccatcggctt gagcgcggat ttttcccgtt tgaaggcggg
     5941 cggtttgccg ctggtggttt ttaccgcgat tgtgggcgga tttatcttgg tgcaaaactt
     6001 tgtcggggtc ggactggcta cggctttggg tttggacccg ctcatcggtc tgattaccgg
     6061 ttcggtgtcg ctgacgggcg gacacggtac gtcaggtgcg tggggaccta attttgaaac
     6121 gcaatacggc ttggtcggcg caaccggttt gggtattgca tcggctactt tcgggctggt
     6181 gttcggcggc ctgatcggcg ggccggttgc gcgccgcctg atcaacaaaa tgggccgcaa
     6241 accggttgaa aacaaaaaac aggatcagga cgacaacgcg gacgacgtgt tcgagcaggc
     6301 aaaacgcacc cgcctgatta cggcggaatc tgccgttgaa acgcttgcca tgtttgccgc
     6361 gtgtttggcg tttgccgaga ttatggacgg cttcgacaaa gaatacctgt tcgacctgcc
     6421 caaattcgtg tggtgtctgt ttggcggcgt ggtcatccgc aatatcctca ctgccgcatt
     6481 caaggtcaat atgttcgacc gcgccatcga tgtgttcggc aatgcttcgc tttcgctttt
     6541 cttggcaatg gcgttgctga atttgaaact gtgggagctg accggtttgg cggggcctgt
     6601 aaccgtgatt cttgccgtac aaaccgtggt gatggttttg tacgcgactt ttgttaccta
     6661 tgtctttatg gggcgcgatt atgatgcggc agtattggct gccggccatt gcggtttcgg
     6721 cttgggtgca acgccgacgg cggtggcaaa tatgcagtcc gtcacgcata ctttcggcgc
     6781 gtcgcataag gcgtttttga ttgtgcctat ggtcggcgcg ttcttcgtcg atttgattaa
     6841 tgccgcgatt ctcaccggtt ttgtgaattt ctttaaaggc tgattttccg cctttccgcc
     6901 aaagcacctg caaggtttac cgcctgcagg tgcttttgct atgatagccg ctatcggtct
     6961 gcaccgtttg gaaggaacat catgtatcgg aaactcattg cgctgccgtt tgccctgctg
     7021 cttgccgctt gcggcaggga agaaccgccc aaggcattgg aatgcgccaa ccccgccgtg
     7081 ttgcaaggca tacgcggcaa tattcaggaa acgctcacgc aggaagcgcg ttctttcgcg
     7141 cgcgaagacg gcaggcagtt tgtcgatgcc gacaaaatta tcgccgccgc ctacggtttg
     7201 gcgttttctt tggaacacgc ttcggaaacg caggaaggcg ggcgcacgtt ctgtatcgcc
     7261 gatttgaaca ttaccgtgcc gtctgaaacg cttgccgatg ccaaggcaaa cagccccctg
     7321 ctgtacgggg aaaccgcttt gtcggatatt gtgcggcaga agacgggcgg caatgtcgag
     7381 tttaaagacg gcgtattgac ggcagccgtc cgcttcctgc ccgtcaaaga cggtcagacg
     7441 gcatttgtcg acaacacggt cggtatggcg gcgcaaacgc tgtctgtcgc gttgctgcct
     7501 tacggcgtga agagcatcgt gatgatagac ggcaaggcgg tcaggaaaga agacgcggtc
     7561 aggattttga gcggcaaagc ccgtgaagaa gaaccgtcca aacccacgcc cgaagacatt
     7621 ttggaacaca atgccgccgg cggcgatgtg ggcgtacccc aagccgcaga aggcgcgccc
     7681 gaaccggaaa tcctgcatcc tgacgacggc gagcgtgccg ataccgttac cgtatcacgg
     7741 ggcgaagtgg aagaggcgcg cgtacaaaac cagcgtgcgg aatccgaaat taccaaactt
     7801 tggggaggac tcgataccga cgtgcaaaaa gagttggtcg gcgaacaacg caagtgggcg
     7861 caggaaaaaa tcagcaactg ccgacaagcc gccgcgcagg cagaccggca ggaatacgcc
     7921 gaatacctca agctgcaatg cgacacgcgg atgacgcgcg aacggataca gtatcttcgc
     7981 ggctattcca tcgattaggg gcaaaccgat gactaccgtc cccaaaagca ggattcccgt
     8041 caaaccgctg cccgaaaaaa ccacagacga agccaaagtc gaaaaatggc ggcagctcgg
     8101 tgcggaacac ggtttgtcgg gcgaatgggc agttgccgcc agattgggcg agaacggttt
     8161 taccgaagaa cagatggaaa atatcgccaa cctgttcggc agataaagag aaaattgacg
     8221 gaaatgccgt ctgaaaccct gttatcggtt tcagacggca ttttgaccaa tacggtacgc
     8281 aggcgcaaaa cagccggctt ttcctgtgtt acctatgctg atgtttcaac acacaggacg
     8341 atacaaaaaa cgtcgcccta tgtgccgtcc tgattcggaa gggttacgct ccttc
//
LOCUS       DAWFJW010000082         8280 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFJW010000082 DAWFJW010000000
VERSION     DAWFJW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.082"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(265..1650)
                     /gene="pntB"
                     /locus_tag="WGR19_001530"
     CDS             complement(265..1650)
                     /gene="pntB"
                     /locus_tag="WGR19_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0799035.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFIGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1716..2099)
                     /locus_tag="WGR19_001531"
     CDS             complement(1716..2099)
                     /locus_tag="WGR19_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0799036.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2103..3644)
                     /locus_tag="WGR19_001532"
     CDS             complement(2103..3644)
                     /locus_tag="WGR19_001532"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0799037.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTASIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMIVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KSEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            3933..4766
                     /gene="serB"
                     /locus_tag="WGR19_001533"
     CDS             3933..4766
                     /gene="serB"
                     /locus_tag="WGR19_001533"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0799038.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAVGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4832..6055
                     /locus_tag="WGR19_001534"
                     /pseudo
     CDS             4832..6055
                     /locus_tag="WGR19_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6215..7795
                     /gene="purH"
                     /locus_tag="WGR19_001535"
     CDS             6215..7795
                     /gene="purH"
                     /locus_tag="WGR19_001535"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0799039.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEIPLEAGANRFEFKRVGGGLLVQTPDIHR
                     ISRSDLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1849 a         2262 c         2346 g         1823 t
ORIGIN      
        1 caagtggaaa aatacttggc agattatcag gcacacttgg aaaaagtgtt cggctaatta
       61 aaaatccatc ttcaacacgg agatggattt tgtttgtttc gttgattttg tgtcagtttc
      121 agatgtaggt gcttattcgg acgggccgtc tgaaaatgtt tgccccaatg caaaaaaatc
      181 actgcaaacc ttcataaacg gggtttgcag tgattttttc aaatcaaaca gattgaaaac
      241 ctgcgccgaa ttgttcagac ggcattattt tttcagttcg gacagaatgt catctacggt
      301 tttcttcgca tctccgaaac acatcacgct gttttcgttg aagaacagtg ggttttgtac
      361 acctgcgtag ccggtattca tcgagcgttt gaagacgacg acttcttttg ccttccacac
      421 ttccaacacg ggcatacccg caatcgggct gttcgggtcg gtttgggcgg caggattgac
      481 ggtatcgttc gcaccaatca ccaaaaccac atcggtctcg gggaagtcgt cgttgatttc
      541 gtccatttcc aaaacgatgt cgtaggggac tttggcttcg gcgagcagta cgttcatatg
      601 accgggcagg cggccggcga cggggtggat gccgaagcgt acttctgtgc cgtttttacg
      661 caaaagctcg gtgatttcgg caacggggta ttgcgcttgt gcgactgcca taccgtagcc
      721 cggggtaatg atgacactgt ttgcgccttt cagcatttcg gcaacatcgg cagctttgac
      781 ttctcggtat tcccctatct cttggctgcc ggaagatgat gtgccgctgt cgctgccgaa
      841 accaccggca attaccgaga caaacgagcg gttcatggct ttgcacataa tgtaggacag
      901 aatcgcgccg cttgagccga ccagcgcgcc ggtaacgatg agcaggtcgt tggagagcat
      961 gaagcctgcc gctgcggccg cccagccgga gtaggagttg agcatggaca cgaccacggg
     1021 catatctgcg ccgccgatgg aggcgaccaa gtgccagccg aatgcgaggg cgatcagagt
     1081 cataatcagc aggatgaagc cgctgccgtc aatgccgata aatacgagca gcaacacaaa
     1141 cgatacggca agtgccagtg cgttgagctt gtgtttggcg ggcagttgca gcgggctgct
     1201 gctgattttg ccgttgagtt tgccgaatgc gaccagcgag ccggtaaagg ttaccgcgcc
     1261 gatgaaaatg cccaaataca cttcgaccag atggatggtg tgcatatcgt gcgaaacgtt
     1321 gcccggctcg atgtagctgt tgaagccgac caaaaccgcc gccaagccga cgaagctgtg
     1381 cagcagggca atcagttcgg gcatttcggt catttccacc tttttggctt tgtagatgcc
     1441 gattgccgcg ccgatgagca tggcgatgat gatccagccc agtccgtggg tattgtcgga
     1501 aaaaacagtt acaaaaaggg cgaccgccat accggcgata ccggaatagc agccctgttt
     1561 ggcggtttcc tgtttggaca gccccgccag tgagaagatg aataaaattg cggcaacgat
     1621 atacgccgct gttacgagtc ctgaagacat ggaaattctc cgattttcga tgatttgttt
     1681 ttaatgccgt ctgaactgtt cagacggcat ctgtttcaag cagccgcgac aaacagcccg
     1741 acggtgcagg caaatccgcc cgcccacatc agcgagcgca atgctgcttt gtctgcgatg
     1801 tagcaccaga taaaggcgag gcggaacagg atgaacaggc cggcaagcgt gttgacggtt
     1861 gcttgtccgg cattgccggt tgcgtgtgcc gtcaaaacgg cggcggcaaa cggtgcaaag
     1921 gcttcaaaac cgttttgctg cgcggcgtgg gcacgggcgg ctgtgccttg cgtgcgcgcc
     1981 agaaaatcgc gcggattgtg gttgtcttta aaccggaatc cgcccgcttt tttggcatat
     2041 gccgcacaaa aaagcggcaa taggcaggca atcagaatgc accaataggc gaaagtcatg
     2101 ctttatcctt tcctaaacat attcaacatg cggcgcgtta cgaagaagcc gccgaagatg
     2161 ttgatgctgg caatcaggat ggcaacaaac gacagcagcg aaacgaagcc gttgccctga
     2221 ccgatttgca gcagcgcgcc gacgacgatg atgccggaga tggcgttggt taccgacatc
     2281 agcggtgtgt gcagcgagtg gctgacgttc cagacgacgt agtagccgat gacgcaggcg
     2341 aggacgaaca cgataaagtg gttcaggaat gcggcgggtg cgaccgcgcc gacccacagt
     2401 accaacacgg cggcgatgac ggcgggcgcg agttttttcc acaggggaac gggtttcggc
     2461 tcggacttgg cggcaggcgc ggctttttca gacggcgttt gctgcggctg ggcggaaacc
     2521 tgaatcggcg gaggcgggaa ggtgatttcg ccgtcgcggg taacgatcat gttgcggata
     2581 atcacgtctt caaaatccag cgtgatttcg ccgtctttgt tcgggcttaa cagcttggtc
     2641 aggttgacca agttggtggc gtaaagctgg gaagactgtc cggcaaggcg gtttgccatg
     2701 tcggtgtagc cgatgatttt cacgccgttg ccggttacgg acaattcgcc cgggcgggtg
     2761 agttcgcagt tgccgcccgt cgccgccgcc aaatcgacga tgacggagcc ggatttcatg
     2821 ctttccacca tttctttggt aatcagcttg ggcgcgggtt tgcccggaat ggaggcggta
     2881 gtgatgatga tgtccacttc tttcgcctgc tcggcaaaga gcttcatctc ggcggcgata
     2941 aattcgtcgc tcatcacttt ggcgtagccg tctccgctgc cgcccgattc ttgcgggaag
     3001 tcgagtttca aaaacttgcc gcccatcgat tcgatttgtt ccgccacttc caagcgggta
     3061 tcgaacgcgc gcaccactgc gccgagcgag tttgccgtac cgatcgccgc taaacctgcc
     3121 acaccggcgc caatcaccaa aacctgcgcg ggcggcactt tgcctgcggc agtaatttga
     3181 ccggtgaaga aacggccgaa ggcgttggcg gcttcaatca cggcgcggta gccgctgatg
     3241 tttgccattg aagacaaagc gtccaaagcc tgcgcgcgcg aaatgcgggg caccatatcc
     3301 atcgccagcg cgttcacttt cttggcgcgc aaggcttcga ccaaagcctc gttttggcgc
     3361 ggccacagga agctgacgat ggtttgacct tcgttcaaaa gcggcagttc ctgttcggac
     3421 ggcgcgttga ccttataaat caaagggcag gcccaaaccg ccgctttgtc ggcaacggtt
     3481 gcgcctgctg tttggtaagc ggcatcgtcc aaacttgccg ccaaacctgc accgctttcg
     3541 acaacggttt caaagcccag tttgcccaac agggcaacgg tggcgggcgt gcaggcgaca
     3601 cgggtttcgc cggataatga ctcgcgtggg ataccgattt tcatctctga atcctttttc
     3661 gggttgttta tatgtatcgt gggttaaatt taaatcgggg ggggcggagc aacgccgtac
     3721 cggtttaaag ccgactcact tcaaatgtta atatatttta gataatcccc ttataacgaa
     3781 ttttcatcag gctggcaata gttgcggcat tttcccgtgt tgtccgacac atactgccgt
     3841 ttgtgtttga agtcattttc cgattgcagg cccgcccgcc gtaacattct ttgccgtttc
     3901 actatataat ccgcattttt tgagccgccg ttatgccgca agccctcgtc ctccaatttc
     3961 cctccgccga agccctgcct tccgacttcc cctcccgcct gcccgaacct gattgcgccg
     4021 atgaaaagcg tatgcgtttt atcgttgaag aagggttttc tttaagcggg gcggacgcgg
     4081 cgttgcttgg cagccgtcaa atcgaccacg ccgtgttgcc ggatatggat ttcggcgaac
     4141 tcggtttgat tgtcagcgat atggattcga cgctgattac catcgaatgc gtcgatgaaa
     4201 ttgcggcagg cgtgggttta aaagacaagg tagcggaaat taccgagcgt tcgatgcgcg
     4261 gagagttgga tttcgagcag tcgttgcgca gccgcgttgc gctgttggcg ggattggacg
     4321 aacagatttt ggcggacgtt tatgaaaacg ttttgaagct ctcgcccggt gcggaatttt
     4381 tgttggacga atgcaaaagg cacgatgtga aattcctgct ggtgtcgggc ggcttcacgt
     4441 tttttaccga acgtctgcaa caacgcctcg gcttcgaata ccaacacgcc aatattttgg
     4501 aaattgaaaa cggtaggttg accggccgtc tgaaaggcag aatcatcgac gcgcaggcaa
     4561 aggcggattt gttgcgcgaa taccgcagcc gcctcggatt gcagccgcat caggtgttgg
     4621 cggtgggcga cggtgcgaac gatattccga tgctcaaaga agcgggcata ggcgtggctt
     4681 accgtgccaa accgaaagcg cgggccgccg ccgatgcctg tatcaacttc ggcggtttgg
     4741 agcgtgtacg cggcctgttc ggataggcgg ataggaaacg gatgccgtct gaaaaacgtg
     4801 ttcagacggc atttgaacgg caggaacgac agtgggacgc agaaaacttt ggtttgccct
     4861 ggcagcagcg ggcgttatcg gcggtttggt cggcattgtg ctgacggaac tgatgcactt
     4921 catacaacac acagcatacg gttatggcgc ggacggcgtg tacacttcgt tccgcgaagg
     4981 cgtggcacag gcttccggta tgcggcgcgt tgccgtgctg acgctatgcg gcgcgatcgc
     5041 aggcggcggc tggtggctgc tcaaacgttt cggcaagccg caaatcgaaa tcaaagccgc
     5101 cttgaagcag ccgttgcagg ggctgccgtt tctgacgacg gttttccatg ttctgctgca
     5161 aatcataacg gtcggactcg gttcaccgct cggacgggaa gtcgccccgc gtgaaatgac
     5221 tgccgcgttt gcttttgccg gcggcaggcg gttgggtttg gacgaagacg aaatgcggct
     5281 gctgattgct tgcgcctcgg gcgcgggttt ggcggcggtg tataacgtgc cgctcgcctc
     5341 cacgctgttt atcctcgaag ccatgctggg cgtgtagacg cagcaagccg ccgccgccgc
     5401 attgctgact tcggtcattg ccaccaccgt cgcgcgcatc ggcttaggcg acgtgcagca
     5461 atatcatccc gccaaccttg ccgtaaacac ttcattactt tggttttccg ccgtcatcgg
     5521 cccgatactg ggcgcaaccg ccgtcttttt ccagcgcacc gcccaaaaat tccccttcat
     5581 caagcgcgac aacatcaaaa tcattccctt ggcagtctgc atgtttgcgc tcatcggcgt
     5641 gattgccgtt tggtttcccg aaattttggg caacggcaaa gcaggcaacc aactgacctt
     5701 tggcggtttg accggttggc aacacagcct tgagctgacc gccgtcaaat ggctggttgt
     5761 cttgatggcg ttggcggcag gcgcatacgg cggactgatt acgccgtcta tgatgctcgg
     5821 cagcaccatt tcttttgcag ccgccgccgc gtggaacagc gttttccccg aaatgccgtc
     5881 tggaagcgcg gcagtcgtcg gagcggcggt gtttctcggc gtatcgctca aaatgccgct
     5941 gaccgccatt gtgttcgtat tggaactgac ctacgcgccg ctgtccctgc tgctgccttt
     6001 gtgcatcggc atggcggggg cattgggaac atcgggaaaa atgggcttca aatgaatgtc
     6061 gacaaatgcc gtctgaaagg ttttcagacg gcatttcttt gtaaattgtc aacaatatct
     6121 gcaaaaatct actgccaaaa aactgaaaat agcggataat accgccccga aaaagttccc
     6181 ctttccccat ccaactgtca gaaaaggaag cccaatgtcc gtcatcaaac gtgccctgat
     6241 cagcctgtcc gacaaggcag gcgcggtcga atttgcccaa actctgcaca agctcggtgt
     6301 cgaaatcctc tccaccggcg gcacggcgaa actgcttgcc gatgcgggcg ttcccgtgat
     6361 tgaagtcgcc gactataccg gcttccccga aatgctcgac ggccgcgtga aaacgctgca
     6421 tccgaaaatc cacggcggta ttttgggtcg gcgcgatttg gacgaacacg ttgccaagat
     6481 ggaagaacac ggtatcggca acatcgacct tgtgtgtgtc aacctctacc ccttcgccgc
     6541 caccatcgcc aaaccaaact gcacactgga agacgcgatt gaaaacatcg acatcggcgg
     6601 cccgactatg gtgcgttccg ccgcgaaaaa ctggaaacac gtcgccatcg ttaccgacac
     6661 cgccgacttc cccgccattg ccgccgaact cgaagccaac aacggcgcat tgagcgacaa
     6721 aacccgtttc aacctctcac gcaaagcatt cagccacacc gcccaatacg acggcatgat
     6781 ttccaactac ctgacctccc tttcagacga cgttttgagc ggccagcccc aaatcggcga
     6841 attcccaagc cagttcaacc aaagctggat caaagtgcaa gacatgcgct acggcgaaaa
     6901 cccgcaccaa cgcgccgcgt tctaccgcga tattgacccc gccgcaggca gcctttccgc
     6961 ttataaacag cttcaaggca aagaattgtc ttacaacaac atcgccgatg ccgatgccgc
     7021 atgggaagcc gtcaaatcct ttgacgcgcc agcctgcgtg attgtgaagc acgccaatcc
     7081 gtgcggcgtc gccgttgcag ccgatacatt gaccgcctac aaactcgcct acgtcaccga
     7141 caccaccagc gcattcggcg gcatcatcgc tttcaatcgc gaagtcgacg gcgaaaccgt
     7201 gaaacagatt accgacaacc aatttatgga agttttgatg gcgccgaagt ttaccgccga
     7261 agccctcgaa atcgccgccg ccaagaaaaa cgtgcgcgta ttggaaatac cgctcgaagc
     7321 aggcgcgaac cgcttcgaat tcaaacgcgt cggcggcgga ttgttggtac aaacccccga
     7381 catccaccgc atcagccgct ccgatttgaa agtcgtctcc aaacgccaac cgaccgagca
     7441 ggagtggaac gatttgctgt ttgtctggaa cgtcgcaaaa tacgtcaaat ccaacgccat
     7501 cgtcttcggc aaaggcggtc aaacctacgg cattggcgca ggccaaatga gccgcgtgga
     7561 cagcacccgc atcgccgccc gcaaagcgca agatgccggt ttcgacctca acggcgcgtg
     7621 tgccgcatcc gatgccttct tcccattccg cgacggtgtg gacgtgattg ccgagcaggg
     7681 catcaaagcc atcatccatc cggcaggttc gatgcgcgat caggaagtct tcgacgcagc
     7741 ggacgaacac ggcatcgcca tggtcgtaac cgacgtgcgc cacttccgcc attaacgcag
     7801 atgaacaagg caatgccgtc tgaagggctt tcagacggca ttttgcgcta ttttgtgaag
     7861 gtagggatga cggttcgggt attcctgaca gggtggattt tcaaggtgtt gtatagggtg
     7921 taggaggatt cgtaaaaggt gggatgcagg gtgtgcttca gcccgctgca tcaaaaattt
     7981 ttggagaatc ggcgggagtc ggcggttttg gtttcggcgg ggacggtgga aatgggtaac
     8041 attgacggaa tcgacggaag cggtgggctt cagcccaccg cttgtatatt ggaatcaccg
     8101 tatcatagcg ccacccgcgc ccacccaagg cacacaaccg ttgcgtagct cagggagcgg
     8161 cagggcaacc catcgacaca accggacagt tgccggacaa cacaaccgaa tgcaaggcag
     8221 gttgatgatg agtacccgat accattacgc aggtatagtg aattaacaaa aaccagtacg
//
LOCUS       DAWFJW010000083         8158 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFJW010000083 DAWFJW010000000
VERSION     DAWFJW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.083"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(140..556)
                     /gene="dksA"
                     /locus_tag="WGR19_001536"
     CDS             complement(140..556)
                     /gene="dksA"
                     /locus_tag="WGR19_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0799040.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(703..1539)
                     /locus_tag="WGR19_001537"
     CDS             complement(703..1539)
                     /locus_tag="WGR19_001537"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0799041.1"
                     /translation="MGNEFSQVILNRVEIMNVYFLGGGNMAAAVAGGLVKQGEYRIHI
                     VNRGAEKRERLEKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSV
                     AAGLSVGTLSRYLGGTRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSV
                     GLTVWLEDEAQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGA
                     VALAEQTGEDFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQ
                     YQ"
     gene            complement(1543..1950)
                     /locus_tag="WGR19_001538"
     CDS             complement(1543..1950)
                     /locus_tag="WGR19_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0799042.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1950..2636)
                     /locus_tag="WGR19_001539"
     CDS             complement(1950..2636)
                     /locus_tag="WGR19_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0799043.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2756..3799
                     /locus_tag="WGR19_001540"
     CDS             2756..3799
                     /locus_tag="WGR19_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0799044.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3962..5188
                     /locus_tag="WGR19_001541"
     CDS             3962..5188
                     /locus_tag="WGR19_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0799045.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5474..7624
                     /gene="yccS"
                     /locus_tag="WGR19_001542"
     CDS             5474..7624
                     /gene="yccS"
                     /locus_tag="WGR19_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0799046.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         2001 a         2678 c         1772 g         1707 t
ORIGIN      
        1 ctgtcggata aggtttttta tttctgctaa atgctgcgcc gcctccaaca atcctttcct
       61 ctccctcctc cggctggtgc gcctttgtga atatgctgtc tgaaactcgg ggactcagac
      121 ggcattttgt ttgccgccat cagtcggcaa actgtttttt catcctctct cggcgttctt
      181 gggactcaac agataaagtg gctgtcgggc gtgccagcag ccgcttcaga ccgataggct
      241 ctcccgtatc ggcacagaat ccataatccc cttcatcaat attgcggatg gtcgcctgta
      301 ttttactgag aagttttcgt tcccgatcgc gggtacggag ttccaatgcg tactcttctt
      361 cctgtgtggc acggtcggca ggatcggggg ctgattcgtg ttcttggaga tgccctgtcg
      421 tagcggaagc attttcgatg agttcgtctt gcatttttac cagcaattcg cggaaaaaag
      481 ccaaatgctc gtcattcata taatcgtctt ccggcccgtt ccaattcaaa atatcttgtt
      541 ctgtcagctt tgccataatg tatatcctct cggttcaccg aaaaaaacaa agcaccacaa
      601 agtggcttta ttcctttgat ttttataagg cttattgcct agggcgtttt tgaaaacgcg
      661 catcataaca cgttttggat tggttttttt attttcttta cattattgat attgccgttc
      721 catttcctgc gaacggcgca cacaggcaca aacgccctcg cttatggctt cggcgacacg
      781 atgccgcctg aaagcttcca cggcttcgtg ggttgtcccg ccttttgacg ttacattttt
      841 ttgaagcttc tcgaaatctt cacccgtctg ctcggcaagg gcaaccgctc ctttaaacgt
      901 tgccagactg agcgcgcgtg cttctgccat atcaaaccct tgtcggatgg cggcattttg
      961 caatgcgtcc agcagataaa acacataagc cggtccgctg ccgctgatac cggtaatgct
     1021 gtgcatttgc gcttcatctt ccaaccaaac ggtcaaaccg actgatttca tgattcgatc
     1081 ggcaatcctg cggtctgttt ccgatacttc cgcttcggca aacataccgg atacgcccag
     1141 cccgattttt ccgggtgtat tcggcataac ccggacaatg cggcgtgttc ccccgaggta
     1201 acggctgagc gtaccgactg acaatccggc tgcgacagaa agcaccaatg cgccgttggt
     1261 gcggatattt ttgcacgcgg cttccatatc ctgcggtttg acagcaagga ttaaaacatc
     1321 gtcggaatga agctccggca gggttgccga agtttcgaca cccaactctt tttccaaacg
     1381 ttcacgcttt tccgcacccc ggttgactat atggatgcgg tattcccctt gtttgaccaa
     1441 tccgcccgca acggcagccg ccatatttcc gccgccgaga aaataaacat tcattatttc
     1501 taccctattc aaaattacct gactaaattc attcccaatt ttttaccatc ggacgcgctc
     1561 ggacagaccg ttgcgccgca acccctgctt tttcaaacaa tcaaaacctt cctgtttgaa
     1621 cgtctcaatc aaaggtttgc cgtcacgcgt acgccaatcc aacccgcagg cttcaaactt
     1681 ccctattttc ctgtaaaagt aagattgttg tgcggattca tattcgtttg ccctgacgga
     1741 acgattgccg tctgaaagcg gtatctcgta gtcctctaaa gacggcggac tctcgccgcc
     1801 aatatcccaa aaatcgatgg cggcaggttt tctcggctta caccagccgg acagggacga
     1861 acactcatac caacctgcca accggcagcc tgcaaccgta aatgcaagca acgcagccat
     1921 tgccgtatat tttattgccc gaatttccat cagccccttt tcccgaaaat cgcgctgccg
     1981 atacggacgt gtgtcgcacc gcactcaatg gcggcaggca tatcgtccga catccccata
     2041 gacagcacgt cagccttaac gccagccgta ttgaggtcgg caagcagttt ccgcatcgtt
     2101 tgaaattgca ccttcaactc cgtttcactg ctgttggctt tggcaacaca catcagtccg
     2161 cgtacgacga tattcggcag cttcgccact tccacagcaa gcgcgactgc ttcttcgggc
     2221 gcgacaccgt gcttcaccgc ctcgcccgca atgttcacct caatacacac ctgcaaaggc
     2281 ggcattgagg aaggacgttg cccgctcagc cggacagcgg ttttcagacg gcatacggta
     2341 tgcacccaat gcgcgcgttc ggcgacaaac ttggttttgt tggactgcac atcgccgatg
     2401 acgtgccaca cgatgtcggt caaatccgcc aactcttccg ttttgccgta ccactcctga
     2461 atatagttct cgccgaaatc acgctgtccg gcggcgtaaa cttcgcggat gccgtctgaa
     2521 gggaaagtct taccgacggc aatcaggctg acggaatgcg gcttccttcc cgcctgcaga
     2581 accaattttc caatacggtc ggacacctcc cgataacgtt cttgcaacac cgtcatagat
     2641 tatcccctaa ttaaaaatga ttaaacagtt gaaacccctc cagtcagggc ggtacaatca
     2701 aggttgttag aaccattcca accaatcgaa acattatact aaacagagcc gcattatgca
     2761 gattaccgac ttactcgcct tcggcgctaa aaacaaagca tccgaccttc acctgagttc
     2821 gggcatatcc cctatgattc gggttcacgg cgatatgcgg cgcatcaacc ttcccgaaat
     2881 gagcgcggaa gaggtcggta atatggtaac ttcggtgatg aacgaccacc agcggaaaat
     2941 ctaccagcaa aacttggaag ttgacttctc gttcgaactg cccaacgtcg cccgattccg
     3001 cgtcaacgcc ttcaacatcg gccgcggtcc cgccgccgta ttccgcacca ttcccagcac
     3061 cgtcttatcg ctggaagaat tgaaagcccc gagcattttc caaaaaatcg cagaatcgcc
     3121 gcgcggcatg gtattggtta ccggccctac cggttcgggc aaatcgacca cgcttgccgc
     3181 aatgatcaac tacatcaacg aaacccagcc ggcacacatc ctgaccatcg aagacccgat
     3241 cgaattcgtc catcaaagca aaaaatccct gattaaccag cgcgagctgc accagcacac
     3301 cctcagcttc gccaacgcgc tgcgttccgc attgcgcgaa gaccccgacg ttatccttgt
     3361 cggcgagatg cgcgacccag aaaccattgg cttggcactg accgccgccg aaaccggaca
     3421 cttggttttc ggcacgctgc acacgaccgg cgcagcaaaa accgtcgacc gtattgtgga
     3481 cgtattcccg gcgggagaaa aagaaatggt gcgctctatg ctgtccgaat cgctgaccgc
     3541 cgtcatctcc caaaacctgc tgaaaacgca cgacggcaac ggccgtgtcg cctcgcacga
     3601 aatcctgatt gccaaccccg ccgtccgcaa cctcatccgc gaaaacaaaa tcacgcagat
     3661 taactccgtc ctgcaaaccg ggcaggcgag cggtatgcag acaatggacc aatcgctgca
     3721 atcgctggtg cgccaagggc tgatcgcacc ggaagtcgca cgcagacgcg cgcaaaacag
     3781 cgaaagtatg agtttctgac acacaaccgc cttccggcca taccggcggg aaaacaaggc
     3841 gcaaacacgc ggggcgggac gcagcatccc gcccggctac ctttccgaac aagaagcgtc
     3901 cgccttcctg ttgaaacctg ccgccgcaaa ctgcaaggct taaaccgaaa agaagctaac
     3961 gatgaatacc gataacctgc acgacatctt ggacgaaatg gttcaagtgt attctcaaaa
     4021 aaaacaaagc cgatccgaaa ccccggccga aatcggcgca cacttccacc cgctgctcga
     4081 ccgcctgtgc gaaaccgcag aagcacaaaa cgcgtccgac atccttatca gcaaaggatt
     4141 cccgccctcg ttgaaaatca acagcgcatt aaccccgcag ccgcaaaagg cgctgacggg
     4201 cgaggaaacc gccgccatcg ccgcatcgac gatgaacgcc gaacaatcgg aaatattccg
     4261 gcgcgacggc gaaatcaact actccgtcca gtcgcgcagc ggcacgcgct accgcgccaa
     4321 cgcctaccac agccaaggca gcgcaggttt ggttttgcgg cgcatcaacc acgtcatccc
     4381 gcaaatgcag gaattgggcc tgcccgaaaa actcaaagac ctcgccgtcg caccgcgcgg
     4441 gctgctgatt atcgtcgggc ctaccggttc gggcaaatcc accacgatgg cgactatgct
     4501 cgaacaccgc aacaaaaccc tgcccagcca tatcgttacc atcgaagacc cgattgagtt
     4561 tatctacaaa ccgcgccgct gcatctttac ccagcgcgaa atcggcgtcg acaccataaa
     4621 ctggcagacg gcggtacaaa acgctatgcg ccagtccccc gacgtggtct gcatcggcga
     4681 agtccgcagc agggaaagta tggaatacgc gatgcagctc gcccaaaccg gccacctgtg
     4741 catttttacg ctccacgcca acaccgcgcc gcagtcgctc gaacgcatac tcaacttcta
     4801 ccccaaagaa cagcacaacc aaatactgat cgacatcgcc ctcaacctga ccggcatcat
     4861 ctgccaacgc ctcgccctca aacaagacaa aacgggcagg acggcggttg tcgacttgct
     4921 catcaacacg cccgccatcc aagacttcat cctgaagggc gacctgatga acatcagtaa
     4981 aatcatggaa accgccaaaa ccgacggaat gcagacgatg gatcaaaacc ttttcgaact
     5041 gtaccgtcac ggcatcatca gttacgaaga agccctgcgc cagtccgttt ccgccaacaa
     5101 cctgcgattg cacatccaac tgcacaaaga aggcaaaacg cccgaactcc tttacgacag
     5161 ggtcaacggt ctcaacctca tttcctgatc cgcaaaaccc aatgccgtct gaaaaccgca
     5221 tccccgtttt cagacggcat gattttatcc gccccattca tgtgctacac tttatagtgg
     5281 attaaattta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagcaca
     5341 aatagtacgg aatcgattca cttggtgctt gagcacctta gagaatcgtt ctctttcagc
     5401 taaggcgagg caacgccgta ctggtttttg ttaatccact atattcactt cataacaata
     5461 aaccggtaaa accatgaaaa ccccactcct caagcctctg ctcattacct cgcttcccgt
     5521 tttcgccagt gtttttaccg ccgcctccat cgtctggcag ctaggcgaac ccaagctcgc
     5581 catgcccttc gtactcggca tcatcgccgg cggccttgtc gatttggaca accgcctgac
     5641 cggacggctg aaaaacatca tcgccaccgt cgccctgttc accctctcct cgctcacggc
     5701 acaaagtacc ctcggcacag ggctgccctt catcctcgcc atgaccctga tgaccttcgg
     5761 ctttaccatt ttaggcgcgg tcgggctgaa ataccgcacc ttcgccttcg gcgcactcgc
     5821 cgtcgccacc tacaccacac ttacctacac ccccgaaacc tactggctga ccaacccctt
     5881 catgattttg tgcggcaccg tactgtacag caccgccatc atcctgttcc aaatcatcct
     5941 gccccaccgc cccgtccaag aaagcgtcgc caacgcctac gacgcactcg gtggctacct
     6001 cgaagccaaa gccgacttct tcgaccccga tgaggcagcc tggataggca accgccacat
     6061 cgacctcgcc atgagcaaca ccggcgtcat caccgccttc aaccaatgcc gttccgccct
     6121 gttttaccgc cttcgcggca aacaccgcca cccgcgcacc gccaaaatgc tgcgctacta
     6181 cttcgccgcc caagacatac acgaacgtat cagctccgcc cacgtcgact accaagagat
     6241 gtccgaaaaa ttcaaaaaca ccgacatcat cttccgcatc caccgcctgc tcgaaatgca
     6301 gggacaagcc tgccgcaata ccgcccaagc cctgcgcgca agcaaagact acgtttacag
     6361 caaacgcctc ggccgcgcca tcgaaggctg ccgccaatcg ctgcgtctcc tttccgacgg
     6421 caacgacagc cccgacatcc gccacctgcg ccgtcttctc gacaacctcg gcagcgtcga
     6481 ccaacaattc cgccaactcc agcacaacgg cctgcaggca gaaaacgacc gcatgggcga
     6541 cacccgcatc gccgccctcg aaaccggcag cctcaaaaac acctggcagg caatccgtcc
     6601 gcagctaaac ctcgaatcag gcgtattccg ccatgccgtc cgcctgtccc ttgtcgttgc
     6661 cgccgcctgc accatcgtcg aagccctcaa cctcaacctc ggctactgga tactactgac
     6721 cgcccttttc gtctgccaac ccaactacac cgccaccaaa agccgcgtcc gccagcgcat
     6781 cgccggcacc gtactcggcg taatcgtcgg ctcgctcgtc ccctacttta ccccctccgt
     6841 cgaaaccaaa ctctggatcg tcatcgccag taccaccctc tttttcatga cccgcaccta
     6901 caaatacagt ttctcgacat ttttcatcac cattcaggca ctgaccagcc tctccctcgc
     6961 aggtttggac gtatacgccg ccatgcccgt gcgcatcatc gacaccatta tcggcgcatc
     7021 ccttgcctgg gcggcagtca gctacctgtg gccagactgg aaatacctca cgctcgaacg
     7081 caccgccgcc cttgccgtat gcagcaacgg tgcctatctc gaaaaaatca ccgaacgcct
     7141 caaaagcggc gaaaccggcg acgacgtcga ataccgcgcc acccgccgcc gcgcccacga
     7201 acacaccgcc gccctcagca gcaccctttc cgacatgagc agcgaacccg caaaattcgc
     7261 cgacagcctg caacccggct ttaccctgct caaaaccggc tacgccctga ccggctacat
     7321 ctccgccctc ggcgcatacc gcagcgaaat gcacgaagaa tgcagccccg actttaccgc
     7381 acagttccac ctcgccgccg aacacaccgc ccacatcttc caacacctgc ccgaaaccga
     7441 acccgacgac tttcagacag cactggatac actgcgcggc gaactcgaca ccctccgcac
     7501 ccacagcagc ggaacacaaa gccacatcct cctccaacag ctccaactca tcgctcggca
     7561 gctcgaaccc tactaccgcg cctaccgaca aattccgcac aggcagcccc aaaatgcagc
     7621 ctgaaaaagt ttcggcattt tgtaagagag ggcagattgt cagacaggtt gcaagatagt
     7681 ggataaagct ttgcgcaggg taaatgcgta gcaactgaac cgtcattccc acgaacctac
     7741 atcccgtcat tcccacgaaa acagaaaacc aaaaacagaa acctaaaatc ccgtcattcc
     7801 cacgaaagtg ggaatccagt tcgttcggtt tcgcttgttt taagtttcgg gtaacttcca
     7861 cttcgtcatt cccgcgcagg cgggaatcca atgcgttgag cttcagctat ttagaataaa
     7921 ttttgaaact ctaatcccgt cattcccacg aacctacatt ccgtcattcc cacgaaagtg
     7981 ggaatccaga atcccggact ttcagataat ctttgaatat tgctgttgtt ctaaggtcta
     8041 gattcccgcc tgcgcgggaa tgacgaatcc atccgcacgg aaacctacat cccgtcattc
     8101 ccacgaaagt gggaatccag aatcccggac tttcagataa tctttgaata ttactgtt
//
LOCUS       DAWFJW010000084         8153 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFJW010000084 DAWFJW010000000
VERSION     DAWFJW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.084"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(240..968)
                     /gene="rmpM"
                     /locus_tag="WGR19_001543"
     CDS             complement(240..968)
                     /gene="rmpM"
                     /locus_tag="WGR19_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0799047.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPELEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1332..2282
                     /locus_tag="WGR19_001544"
     CDS             1332..2282
                     /locus_tag="WGR19_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0799048.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2395..3129)
                     /gene="fnr"
                     /locus_tag="WGR19_001545"
     CDS             complement(2395..3129)
                     /gene="fnr"
                     /locus_tag="WGR19_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0799049.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3332..4753
                     /gene="hemN"
                     /locus_tag="WGR19_001546"
     CDS             3332..4753
                     /gene="hemN"
                     /locus_tag="WGR19_001546"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0799050.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5106..6680)
                     /locus_tag="WGR19_001547"
     CDS             complement(5106..6680)
                     /locus_tag="WGR19_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0799051.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            7005..8105
                     /locus_tag="WGR19_001548"
     CDS             7005..8105
                     /locus_tag="WGR19_001548"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0799052.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGEKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAKCFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         2059 a         2287 c         2001 g         1806 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tctgaatctg aatccgaatc cgaatccgaa
       61 tccgaacctg caccccgatt ctgacgatag ccgtttcaga aaaccaccca aaaaaacaaa
      121 ccccgcaaaa gcgggggttt gtttcacatc atcgatgccg accgacgttt cacgaatatt
      181 gcaacagtac ctggtacaaa atccactaac ctcatgcatc ggcaagatat tgcctagcct
      241 tagtgttggt gatgattgtg tgccggcaca acctgacggg ttacgatgct gcggattttc
      301 acatccacac ggcggtcagg ttcgatacat gcaatcagag cctcacgttt tttggcttta
      361 gagactttcg cacccagttt ggcaacttcg gcttcacaaa cttgagtcat ttgcgcttga
      421 gattcgccca agccaacagc agaaattcta gaaacaggta cgccgttgct gaccaggttg
      481 tttgccacta cgtatgcgcg gcgttcggac agggcctgat tgtatttgtc agaacccata
      541 aagtcggtat ggccttcgac gcggacagat tggacattgg ttcgactcag gcgttgcgcc
      601 aatactttca ggttgtcttg agcttcggcg cgcaatgaat ccttatcgaa accgaacagg
      661 gttttggcag acagggaaat ggtttcatca acatattgcg gagcctgctc cacaacgacg
      721 acaggcgcgg gtgcgggttc gggttctagc tcgggttcgg gggcagcaac cgcatcgccg
      781 cattctacgc gaccttggct tgctttatca aagtaggcgt ttttccagca ttcgccatag
      841 ttgttgcgta cgatttcgtt cgactggccg cttacggtgt aaccctgaac ggacgcctcg
      901 cccgcaacag cagtgccgga agcgagcaat gcaacgaata atgcgcttaa tttcagctgt
      961 ttggtcattt tattccctca ttaaatttgt acagcagata cagaagcaac tgctgaattg
     1021 ccaacataca ggccgctgta gaaacatggc ggcaaatcgg atatttcagt ctccactata
     1081 aagaagcagg gggaggactg tcaatactca acgcctgaaa acaaccgtaa aacagcctgt
     1141 ttcctgatag tctatcatgc cgaaaaatca attccgtgtc attactttag ggggattttt
     1201 tccatatcgc acgattgccg ttgcacaaaa acaacaaaca caacggcaaa gccccatacc
     1261 gtaccggcaa gaaaatatga taaattataa acaatgttca gccacccgac acagacccac
     1321 accgacccgc catgaaatta caacaattga aatacgcctt agaagtttac cagcacaacc
     1381 tgaacgtttc cgaagcggcc gaagccttgt ttacttcgca acccggcatc tccaaacaaa
     1441 tcaaattgct ggaagaagaa atcggcattc agatttttat ccgcagcggc aagcgcgtgg
     1501 tttcggtctc gcagccgggc aaggtggttt tggatattgc ggaacgtatt ttacgcgatg
     1561 tacagaacat taaaaatatc ggcagcgagt ttaccggaca ggacagcggt tcgctgacgg
     1621 ttgccacgac gcatactcag gcacgctatg ccctaccctt gattgttgcc gattttgtga
     1681 aacgctatcc gaaagtcaac ctgaccatca aacaggggag ccctgccgcc atcgcacgta
     1741 tggttacctc aggcgaggcg gacttggcga ttgttacgga acggatagac gaccatcccg
     1801 aactgggaaa acttccctgc tatgactgga ctcatgcggt tatcgtaccg aacgaccacc
     1861 ccttgctcga atgcagaaac cccctccgca ttgaagattt ggcgaggttt ccgctgatta
     1921 cttatgaatt tgcattcaat gcgggcagca gcatcgcgcg ggcattttcc aaagcccgtt
     1981 tggaacagcc ggatgtcgca ttggcggcgg cggatacgga cgtattgaag acttatgtgc
     2041 gcttgggttt gggcgtggga ctgatggcga aaatggcgta caacccggat acggacggcg
     2101 atttgcagct tgtggatgcg gcacacctgt tcgagccgtc gccgacgtgg attgctttgc
     2161 gcagcgatac ttatttgcgc ggatatgcct acgactttat ccaagcgttt gcgccgcacc
     2221 tgacacgcga gaaggtggat aggattctgt acacgcccat cagcgaggat ttttcgattt
     2281 aggcggctgc cggttttcag acggcacttt gcggcagata caacaaacag gacagatgtt
     2341 ttcgtctgcc ctgtgtttat tgagaatgct gtctgaaatg ttcgtacggg ttaatcaaat
     2401 ggcgtgcgag cagccggaca ccattttttt caacacctgc agattgagga ttttgatgtg
     2461 cttatgctcg acggaaatca atccttcctg atggaacttg gataatgtgc ggctgacggt
     2521 ttcaagtttc agcccgagat aactgccgat ttcttcgcgg gacattctta agatgaagtc
     2581 gttggcagca aaacctcggg aataaaggcg ttgggaaagg ttcagcagga aggcggcaat
     2641 ccgctcttcg gcgcgcatat tgcccaacag cagcataaca ccttggtcgc gcacgatttc
     2701 acggctcatc atgcggaaga agtgcgtacg caggctgggg atgttttgcc ccagttcttc
     2761 gatgtgggta aacggcagtt cgcacacttc gctgtcttcc aaggcgaccg cgtcgcaact
     2821 gtgcacatgg gaacagatgc cgtccatgcc gatgagttcg cccgacataa agaaacccgt
     2881 tacctgatcg cggccgtcct gactggcgac ggttgttttg aagaagcccg aacggatggc
     2941 aaagagcgag gtaaaggctt cgccgacaca gaacaggtat tcgccctttt tcaggcggcg
     3001 gctttgacgg atgacggcat cgagttggct gagctcgttg ggcagcagcc cgacaggcag
     3061 gcagagttcc cgcaaagaac aggaagaaca cagcgttttc atctgatgtg tagtattatg
     3121 cgaagccata ccgtaccttt ttgtgcgctt tgccccatca tgattatagt ggattaaatt
     3181 taaaccagta cggcgttgcc tcgccttgtc cttatttaaa tttaatccac tatatgtgct
     3241 tattgacaca tatcaagaca ggtttatcat actgtggcat tctaccaaac tatccaaaat
     3301 ttgactaaca tcataaaacc gcacacacac catgaaaatc attcagatac agaacaatca
     3361 caatgtaaac gatgaccgcc ccgagtttga ccgcgcgctg attgccagcc tgcccgccag
     3421 cggcccgcgc tacacttcct accctaccgc cgaccgtttc catgacggtt tccgcgaagg
     3481 cgaatatatc aaagctttac atttgcgcgg tatgggcgcg ttaaacaaac cgctttccct
     3541 ttacattcac attccgttct gcaacaccat ctgctactac tgcggctgca acaaaatcat
     3601 caccaaagac aaaagccgcg ccgatgccta catcgaatat cttgaaaaag aaatggaact
     3661 gctcgctcca catctgaacg gacggcacca gcttgcccaa ctgcacttcg gcggcggcac
     3721 gccgaccttt ttgagcgacg aacagatcga acgtgtcttc cgcatgatac gcaaacattt
     3781 cgagttaatc cccaccggcg aatactccat cgaaatcgac ccgcgcaaag tcagccgcga
     3841 caccgtcctc atgctcggca gactcggctt caaccgcatg agcatcggca ttcaggattt
     3901 cgaccccaaa gtgcaggcgg cggtcaaccg catccaaagt tacgaagaaa ccaaagaagt
     3961 catcgatgcg gcgcgcgaag cggggttcaa atccgtcagc gtcgatttga tttacggcct
     4021 gccgcaccag acttcggaaa gcatcaaaac caccatcgat accgttttgt cgctcgatcc
     4081 cgaccgcctc gccctttatc actacgccca cctgccgcac gtgttcaaac cgcaacgccg
     4141 catcgatacc gccgccgttc ccgacagcga agagaagctc gatatgctgc aatactgcgt
     4201 ccaaacccta accgaacgcg gctacgtctt catcggcatg gatcatttcg ccaaacctga
     4261 cgacgaactc tccatcgccc tcaaagaagg cttcctccag cgcaacttcc aaggctattc
     4321 gacctacgcg gattgcgatt tggtcgccat cggcgtgtcg tccatcggca aaatcggcag
     4381 cacctattcc caaaacgaac gcgacatcga tgcctactat gccgccatcg acgaaggcag
     4441 actgcccatc atgcgcggtt atcagctcaa tcaggacgac atcctgcgcc gcaacatcat
     4501 tcaggatttg atgtgccgtt tcgcgctcga ctaccggatt tacgaaagta tgttcggcat
     4561 cccgttcgac cgctacttca aagacgaact ggcggatttg gaaaaactcg ccggtttggg
     4621 attggtgcgc ctgaacagcc acgggctgac cgttaccccg aaaggacgct tcctcatccg
     4681 caacatcgcc atggtcttcg attaccacct gcgccataaa gaaaccaagg cgaaatattc
     4741 gcaaacggtg tgattgcgtc taacgtacaa atgccgtctg aaaggctttt tcagacggca
     4801 ttttgctgcc ggcaggataa gtgttttcaa gaacaggcgg cggcatatca taacgttccg
     4861 cacctttgtg tccgaccgtt ccgaaaccaa gatatagtgg attaacaaaa accagtacag
     4921 cgttgcctcg ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     4981 tttgttaatc actataccaa acggcatatc ccgacagaac agattgtgca taaggcacaa
     5041 gcccgcacat tccatcaaca aaatgccgtc tgaacacggg ttcagacggc atcagtattt
     5101 tacaatcaga atactgcctg taaaaccaag tagcagacaa ccgacaatac agcggcggca
     5161 ggcagggtaa tgacccacgc caaaccgatg ggcttcatca gtttccagtt ggcattgcgg
     5221 ttgaccagac cgatgccgag taccgcgccg accaagatat gcgtactgga cacgggcagc
     5281 cccatcagcg acgcgcccat cacgacggat gcggcggaca gttcggcggt aaaacccgaa
     5341 gcaggatgca tttccgccaa actcgtaccg acggttttaa tcacctcttt accgacaaac
     5401 cacaaaccga caatcagcgc gataccgaaa gtcagcatcg caatcggggg gacgacattt
     5461 tgcgcggcaa cgctgttggt acgcaaaaca tccataatcg cggcaaacgg accgatggcg
     5521 ttggcgatat cgttcgcacc gtggctgaat gcgaagccgc aggcggtaaa gacctgcatc
     5581 catgaaaaca tctgaaaggt cgatttgccc aagtctttac gcttgaggct tttggcaaac
     5641 acaaacgtcc ccatccacac cgccgcgcct atcataaaga tggtcaggaa gctgttgacg
     5701 ttgctcatcc ccaaatgcag gtttttcaag cccttgaaaa tcagcatagc ggaaatcatc
     5761 atcgcgccga acgaagcgat aaagggaatc caagaatgca gtgccttgta ggaatcgaca
     5821 ttgtttttac ggttgtcgaa cgcataaaga ccgcggtaat actccgattg cagctcttgc
     5881 ggatcgaatt cgggttcgtc gtaaatttgc gcgtcgtgcg ccattttggt ggcgtactcg
     5941 actttttcgg cttcggacaa accctcgaaa aacaggcggt gccgttcttt ataggccttt
     6001 ttttcctgct tgatgccctt gagcgtgcct tccgcccaag cgttgtaatc taagacgttt
     6061 ttcttgacgc gcgaaaacag gaaataggac accgcgccgc ccaacacggg cgacaatacc
     6121 caagaaacac caataccgcc cagcttgccc caacgtatca aatcgcccga tgcggcatcg
     6181 ttcattaccg ccatacatac cgcgctgccg acaatgccgc cgataatgga atgggtggta
     6241 gataccggaa gccctttttt cgaggcaaac aacagccaca acgccgccgc caaaagcgcg
     6301 gacatcataa taaacacaaa ctgtatgggt tcgaaatcaa cacccttcaa atcgacgatg
     6361 cctttgcgta tggtattggt tacctcgccg cccgcgatga ccgcgccgct gacctcaaat
     6421 accgccgcaa tcagcaaagc ctgcgggatg gtcagcgtac ccgcaccgac gctggtgccg
     6481 aaagaattgg caacatcgtt gccgccgatg ttgaacgcca taaacacgcc gaacatcgtg
     6541 gcaataatga ataaggcggc gtaattgtaa tgggtataac ccaagcccca ataaataaaa
     6601 tagccgactg tacctaccag catggcggca aagacggcgt tgatggtttt aacatttgcg
     6661 ctcatttgga tttgagccat ggcaatcttc ctttaatgtg gtggaaatca tgagtgttgc
     6721 cgacactgta ctgctgcctc cggtcgaact gccgtttcgg gatatggcag acgcatcctt
     6781 ccttctgaac cgcatccgaa tacccccaaa cctgaccgaa tgcaaaactg ctgccggaaa
     6841 cggtttgtat gacacttcta ctactggatg ttgcgggcgc attataatat ttttccatcc
     6901 gtcttgaaac atttatttac actttattta cactgcggcg gcaaatcagt atacgagcgt
     6961 caatacacgt taaaatggcg ttttgcacca gtttgggagt gatgatggaa acacagcttt
     7021 acatcggcat catgtcggga accagtatgg atggggcgga tgccgtgctg gtacggatgg
     7081 acggcggcaa atggctgggc gcggaagggc acgcctttac cccctatccc gacaggctgc
     7141 gccgccaatt gctggatttg caggacacag gcacagacga actgcaccgc agcaggatgt
     7201 tgtcgcaaga actcagccgc ctgtacgcgc aaaccgccgc cgaactgctg tgcagtcaaa
     7261 acctcgcgcc gtccgacatt accgccctcg gctgccacgg gcaaaccgtc cgacacgcgc
     7321 cggaacacgg ttacagcata cagcttgccg atttgccgct gctggcggaa cggactcaga
     7381 tttttaccgt cggcgacttc cgcagccgcg accttgcggc cggcggacaa ggtgcgccgc
     7441 tcgtccccgc ctttcacaaa gccctgttcc gcgatgacaa agaaacacgc gcggtactga
     7501 acatcggcgg gattgccaac atcagcgtac tcccccccga cgcacccgcc ttcggcttcg
     7561 acacagggcc gggcaatatg ctgatggacg cgtggacgca ggcacactgg cagcttcctt
     7621 acgacaaaaa cggtgaaaaa gcggcacaag gcaacatatt gccgcaactg ctcgacaggc
     7681 tgctcgccca cccatatttc gcacaacccc accccaaaag cacggggcgc gaactgtttg
     7741 ccctaaattg gctcgaaacc taccttgacg gcggcgaaaa ccgatacgac gtattacgga
     7801 cgctttcccg ttttaccgcg caaaccgttt tcgacgccgt ctcacacgca gcggcagatg
     7861 cccgtcaaat gtacatttgc ggcggcggca tccgcaatcc tgttttaatg gcggatttgg
     7921 caaaatgctt cggcacacgc gtttccctgc acagcaccgc cgaactgaac ctcgatccgc
     7981 aatgggtaga agccgccgca tttgcgtggt tggcggcgtg ttggattaat cgcatccccg
     8041 gtagtccgca caaagcaacc ggcgcatcca aaccgtgtat tctgggcgcg ggatattatt
     8101 attgagcgca aatgccgtct gaaagcccgt tccgccgctc agacggcatt ttt
//
LOCUS       DAWFJW010000085         8147 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFJW010000085 DAWFJW010000000
VERSION     DAWFJW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.085"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(84..308)
                     /locus_tag="WGR19_001549"
     CDS             complement(84..308)
                     /locus_tag="WGR19_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0799053.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(309..1697)
                     /locus_tag="WGR19_001550"
     CDS             complement(309..1697)
                     /locus_tag="WGR19_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0799054.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1783..2604)
                     /gene="prmC"
                     /locus_tag="WGR19_001551"
     CDS             complement(1783..2604)
                     /gene="prmC"
                     /locus_tag="WGR19_001551"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0799055.1"
                     /translation="MTFDKWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2611..3054)
                     /locus_tag="WGR19_001552"
                     /pseudo
     CDS             complement(2611..3054)
                     /locus_tag="WGR19_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3120..4604)
                     /gene="tldD"
                     /locus_tag="WGR19_001553"
     CDS             complement(3120..4604)
                     /gene="tldD"
                     /locus_tag="WGR19_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0799056.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIASLDSAAKVALLNKVETL
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLMGTQSTGNGRRESYASAPMPRMTNTFMENGGYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGKLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4782..4885
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4952..6175
                     /gene="cytX"
                     /locus_tag="WGR19_001554"
     CDS             4952..6175
                     /gene="cytX"
                     /locus_tag="WGR19_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0799057.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGHSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKQREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6172..7272
                     /locus_tag="WGR19_001555"
     CDS             6172..7272
                     /locus_tag="WGR19_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0799058.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGTRQGEHAVAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNQPPEYTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHIFVIGATQIESESQAPVSVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSLARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7293..7910
                     /gene="thiE"
                     /locus_tag="WGR19_001556"
     CDS             7293..7910
                     /gene="thiE"
                     /locus_tag="WGR19_001556"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0799059.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCAAACRGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         1767 a         2488 c         2183 g         1709 t
ORIGIN      
        1 ttaatccact atatatctta ggtttgcatc ggcaaaatat tcaaacacag ccttttttaa
       61 ggaaatccgg atacggcggc gcatcaataa tgcggcggaa tctcgtcgcg cagggaatac
      121 ggctcttgcg cgtcgggatt cctgtcctgc attttttgat acagcagcct caactgagcc
      181 tgttgcaaat ccagcgtctg ccgcaattcc gccaccatcg cgttcaggcc ggcgattacg
      241 tcctcctgaa gcgcggattg gatttccagc tctgtgatgc ggtgttccaa ctcttgaacc
      301 gcgtccattt acagcaccat cgcggcaatc cagccggcaa tcagcagcgg gatgttgtag
      361 tggatgaagg tcgggataac ggaatcgcgg atgtggtcgt gctgcccgtc ggcgttcagc
      421 cccatcgtcg gacccagcgt ggaatcggac gcaggcgaac cggcatcgcc caacgccccc
      481 gccgtgccga caatggcgac ggtggcaagc ggcgaaaaac ccaaaccgac acacaaaggc
      541 acataaatcg cggcaataat cggcaaagtg gaaaaggacg aaccgatgcc catcgttacc
      601 aaaagtccca ccaccagcat cgccaacgcc gccatacctt tgctgttgcc gaatatcgcc
      661 atactgcttt ccaccagcgg ctggatatgc ccggtcgcat tcatcacggc ggcaaaaccc
      721 tgcgcggcaa tcataatgaa gccgaccatc gccatcatct tgataccttc gccgaatacg
      781 tcgtttgcct tgtcgcggtt gatgaccccc aacatcataa atacggcgaa accgagcatc
      841 gcgcccaaca ccagcgagtc ttcatacatc aactggatgg caaaacatac ggcaatggcg
      901 gcggcggcgg ccaggctgcg gtaggcggac ggctgcggac ggtttgccgc atcggcgttg
      961 cccgccgtat cggcattgtt gctttggtac aggcgcggtt tgcggtaatg gacaaacgcc
     1021 agcaggagtc cggtcagcat tcccaacgcg ggaatcgcca ttgccgccat tacgttgatg
     1081 tttttcacat caagctgcgg cgcggcggaa tggatgttgc ccaacaggat ttcgttcaaa
     1141 aaaatcgcgc cgaagccgta aggcaggaac atataggtcg taaccagccc gaaagtaatg
     1201 acgcacgcaa tcagacggcg gtcgattttc aggcggttga acaccaaaag cagcggcgga
     1261 acaatcatcg ggataaaggc gatgtggata ggtatgatgt tctgactcat aatgcccatc
     1321 acaaggatga tggaaagcag cagccatttg accgcgccct cgcccgaacg cacgctgtcg
     1381 ggcataccgc cccggttcag cttgcggacg accgcgccgg caagctgctg cggcaggccg
     1441 gaatgggtaa tcgccattgc aaacgcgccg agcatcgcat aagaaagcgc aatcttcgca
     1501 ccgccttcca aacctttgtt gaacacgggg ataatccccg cctgactgac ctgtcccgcc
     1561 gcatcggcaa tgttttgcag cggcataccc gccaccgcgc cgccgacaaa cgcgccgacc
     1621 gtcaggctca ataccacgtg cacgcgcgac agcgacagca ccagcataac gattacggca
     1681 actacgactg cattcattgt ttatcgctcc aaacctataa atgtttacat atcgaaacac
     1741 atcataaccc aataatggaa aacccgccaa ttttgcaaac aattatttca aatgcttcat
     1801 atacttcccc agcgtaaccc tgtccaaacc cgccaagtcc ggcagggttt ccactcccga
     1861 aaaaccattc tccgccaaca cgccgcgcac cgccgcgccc tgatcgaaac cgtgttccag
     1921 caataaaaaa ccgccttccg ccaaacggtc gggcgcgcct tgcgccaagg tgcggatgca
     1981 gcttaggccg tctgaaaagt cggtcagcgc gatttgcggc tcaaaccgca aatcgccttg
     2041 caacaaatgt ttatcgccgt tttcgatata gggcgggttg gacacgatga tgtcccattt
     2101 cccttcagac ggcatatcgg tgtcgaacca cgaaccgtgt gcaaattcga cccgcgcgcc
     2161 caaatctgcc gcatttttcc gcgccgtttc aagggcgggc gtgctgatgt cggatgcgcg
     2221 cacaaacgca tcggggcgtt cgagtgcgac ggttacggca accgcgccgc tgcccgtccc
     2281 caaatcccac acgcgcccgt tttcgggcag gcgcgccaat acggcttcga ccaagtgttc
     2341 ggtttcgggg cgcggaatca gcacgctcgg attgactgta aagcgtctgc cataaaattc
     2401 gcgcacacct aaaatatagg caaccggctc gccgttcaga cggcgttgcg ccagcctgtc
     2461 cgcccgctgt cggacttcgt ccggcatttc ttccccgccc cgcgtcaaca actgcacgcg
     2521 cgtatattcc gaaacatatt gcagcagcat tcttgcttca attttaggca gttttgacaa
     2581 gcccaaccat ttatcaaacg tcatttttat cccgtctgcc gctgatgcgg cttttctttc
     2641 cttattcttt ccggcaaacg taccgatggt ggcaaccgca aatgcggcat accacaaata
     2701 aaatcctgca ccgtagcgca caatatccga tgtattccct gcttcatcga cgtatacggc
     2761 tttcacactg aaagccacca acgccaagcc ccaaagtgcc gcatggacag gcacgacctt
     2821 cttccgcaac gccagcaaaa caatggccgc caaccaaaca taattcgcat agaccgcaca
     2881 atacctgata tccaaagaag caaatatcga ccccaaaatc aaaacggtca aaccctccat
     2941 gcttccatga ttgcccaaat aaaatgcaac attggataaa gacgctatcc acagggcaac
     3001 cgacaccagc aacatcacta tgggaaaact tgatttccga ttctgttcct gcatggtttt
     3061 atcctaatgt aaaaggccgt ctgaaaaaac tttcagacgg catcgtgtcg gattcagcgt
     3121 cagattgcgc tgccgccgac ggtcagcccg gcatcgatac gcaaagtcgg ctgccccacg
     3181 ccgacgggga cgctctgccc ttctttgccg cacacgccga caccgctgtc caacgcggta
     3241 tcgttgccta tcatggaaac atgtttcagc acttcggggc cgttgccgat gatggtcgcg
     3301 cctttgacgg gatattgcag tttgccgcct tcgacccacc acgcttcgga tgcactgaac
     3361 acaaacttgc cgctggtgat gtccacttgt ccgccgccga agttgacggc gtaaatgccc
     3421 ttgtcgatgg acgcaatgat ttcttccggc tcatagccgc cgttttccat aaaggtattg
     3481 gtcatgcgtg gcataggggc ggaagcgtaa ctttcgcggc ggccgttgcc ggtcgattgc
     3541 gtacccatca gacgggcgtt ggtttcgtcc tgcatatagc cgactaaaat gccgtcttca
     3601 atcaatacgg tgcggcgggt ttcgttgcct tcgtcgtcga tgttgagcga accgcgccgg
     3661 ccggcaatat cgccctgatc gacgacggta acgcctttgg cggcgacgcg ctcgcctatt
     3721 ctgccggaaa agacgctggt ccccttgcgg ttgaaatcgc cttccaaacc gtgtccgacc
     3781 gcttcgtgca gcaacacgcc cggccagccg ttgcccaaaa cgacggtcat ttcgccggcg
     3841 ggcgcggggc gggattcgag gttggtgagt gcctgtttga cggcggcatc gacaaactgc
     3901 cgcaccaagt tttcatcgaa ataagccaag tcgtagcgtc cgccgccgcc cgcgctgccc
     3961 tgttcgcggc gttcgccctg tttggcgatg acggtaacgt tcaggcgcac catcgggcgg
     4021 atgtcggcgg cgtgtttgcc gtccagacgg gcgaggtaaa ccatatcgta ttcgcaggtc
     4081 aaacccgcca tcacttgcac gatgcgcgga tcggcggctt tggcgagcgt ttccactttg
     4141 ttcaacagcg cgactttggc agcggaatca aggctggcaa tggggtcgga cgcggaacaa
     4201 accggcttgc cgcgcgtttc agacggcatt ttggcggaca ccttgccgcc tgccgcccca
     4261 atcgcgcgga cggcgcgggc ggaacggttt atcgaatcga tgcacaggct gtcggcgtag
     4321 gcaaaggcgg ttttgtcgcc cgaaacggcg cgcacgccca cgccctgatc gatttggaag
     4381 ctgcccgatt tgacgatgcc ctcttccaaa tgccagcttt cataggcggt gcgctggcag
     4441 tagatgtcgg cgtaatcgac gtggtgcgcg ccgatgatgc acaggctttt ggcgagcagt
     4501 tcgggggaaa ggcggttggc ttcgagcagc cgcgcctgta cggcggaata ggtcggatgc
     4561 atagtgtcgg cgcataaaaa atcaggggct tgattatacg gcatttgtta tatagtggat
     4621 taacaaaaaa cagtacggtg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
     4681 cgtcgccttg tcctgatttt tgttaatcca ctatagaaat gcgccgtgcc gccgaaaatg
     4741 taagaatttt gccaacgcac gttgctttgt gtaaacttaa agctttcttg tcggagtgcc
     4801 gccgccgggc ggctgagatt gcgaaagcag aatccgtaga acctgtcggg gtaatgcctg
     4861 cgtaggaaac aaaccgtcaa atgccttatc aggcttccgt tcccttttcc gcacttcccc
     4921 gcccaccttc cttcttttta aaggacgtca tatgtcgggc aatgcctcct ctccttcatc
     4981 ttccgccgcc atcgggctga tttggttcgg cgcggcggtc tcgattgccg aaatcagcac
     5041 gggtacgctg cttgcgcctt tgggctggca gcgcggtctg gcggctctac ttttgggtca
     5101 tgccgtcggc ggcgcgctgt tttttgcggc ggcgtatatc ggcgcactga ccggacacag
     5161 ctcgatggaa agcgtgcgcc tgtcgttcgg caaacgcggt tcagtgctgt tttccgtggc
     5221 gaatatgctg caactggccg gctggacggc ggtgatgatt tacgccggcg caacggtcag
     5281 ctccgctttg ggcaaagtgt tgtgggacgg cgaatctttt gtctggtggg cattggcaaa
     5341 cggcgcgctg attgtgctgt ggctggtttt cggcgcacgc aaaacaggcg ggctgaaaac
     5401 cgtttcgatg ctgctgatgc tgttggcggt tctgtggctg agtgccgaag tcttttccac
     5461 ggcaggcagc accgccgcac aggtttcaga cggcatgagt ttcggaacgg cagtcgagct
     5521 gtccgccgtg atgccgcttt cctggctgcc gcttgccgcc gactacacgc gccacgcgcg
     5581 ccgcccgttt gcggcaaccc tgacggcaac gctcgcctac acgctgaccg gctgctggat
     5641 gtatgccttg ggtttggcag cggcgttgtt caccggagaa accgacgtgg caaaaatcct
     5701 gctgggcgca ggtttgggtg cggcaggcat tttggcggtc gtcctctcca ccgttaccac
     5761 aacgtttctc gatgcctatt ccgccggcgc gagtgcgaac aacatttccg cgcgttttgc
     5821 ggaaacaccc gtcgctgtcg gcgttaccct gatcggcacg gtgcttgccg tcatgctgcc
     5881 cgttaccgaa tatgaaaact tcctactgct tatcggctcg gtatttgcgc cgatggcggc
     5941 ggttttgatt gccgactttt tcgtcttgaa acagcgtgag gagattgaag gctttgactt
     6001 tgccggactg gttctgtggc ttgcgggctt catcctctac cgcttcctgc tctcgtccgg
     6061 ctgggaaagc agcatcggtc tgaccgcccc cgtaatgtct gccgttgcca ttgccaccgt
     6121 atcggtacgc cttttcttta aaaaaaccca atctttacaa aggaacccgt catgacccgt
     6181 atcgccatcc tcggtggcgg cctctcggga aggctaaccg cattgcagct tgcagaacaa
     6241 ggttatcaga ttgcactttt cgacaaaggc acccgccaag gcgaacacgc cgtcgcttat
     6301 gttgccgccg ccatgctcgc gcctgcggcg gaagcggtcg aagccacgcc tgaagtcatc
     6361 aggctgggca ggcagagcat tccgctttgg cgcggcatcc gatgccgtct gaacacgcac
     6421 acgatgatgc aggaaaacgg cagcctgatt gtgtggcacg ggcaggacaa gccattatcc
     6481 agcgagttcg tccgccatct caaacgcggc ggcgtagcgg atgacgaaat cgtccgttgg
     6541 cgcgccgacg acatcgccga acgcgaaccg caactcggcg gacgtttttc agacggcatc
     6601 tacctgccga ccgaaggcca gctcgacggg cggcaaatat tgtctgcact tgccgacgct
     6661 ttggacgaac tgaacgtccc ttgccattgg gaacacgaat gtgcccccga agacctgcaa
     6721 gcccaatacg actggctgat cgactgccgc ggctacggcg caaaaaccgc ttggaaccaa
     6781 cctcccgaat acaccagcac cttgcgtggc atacgcggcg aagtggcgcg ggtttacaca
     6841 cccgaaatca cgctcaaccg ccccgtgcgc ctgctgcacc cgcgttatcc gctctacatc
     6901 gccccgaaag aaaaccacat cttcgtcatc ggtgcgaccc aaatcgaaag cgaaagccaa
     6961 gcccccgtca gcgtgcgttc cgggctggaa ctcttatccg cactctatgc cgtccacccc
     7021 gctttcggcg aagccgacat cctcgaaatc gccaccggcc tgcgccccac gctcaaccac
     7081 cacaaccccg aaatccgtta cagcctcgcc cgacgcctga ttgaaatcaa cggccttttc
     7141 cgccacggct tcatgatttc ccccgccgta accgccgccg ccgtcagatt ggcagtggca
     7201 ctgtttgacg gaaaagacgc gcccgaacgt gatgaagaaa gcggtttggc gtatatccga
     7261 agacaagatt aaagccgtct gaaaggaacc ccatgacctt cccgccactc aaatccccgc
     7321 tcaaattcta cgccgtcgtc cccaccgccg attgggtggg gcgcatggtc aaagcaggtg
     7381 ccgacacggt gcaactgcgc tgcaaggccc tgcacggcga tgaattgaaa cgcgaaatcg
     7441 cccgctgcgc cgcagcctgc cgtggcagcc gtacgcagct tttcatcaac gaccactggc
     7501 gcgaggcaat cgaagcaggc gcgtacggcg tgcatctcgg acaagaagac atggacaccg
     7561 ccgaccttgc cgccatcgcc gccgccggtt tgcgcttggg tttgagtacg cactccgttg
     7621 ccgaactcga ccgcgccctg tccgtacacc ctagctacat cgccagcggc gcgattttcc
     7681 cgaccacgac caagcaaatg cccaccgccc cgcaaggctt ggacaaactg tgcgagtatg
     7741 tgaaacaggc aggcggcacg cccgtcgtcg ccatcggcgg tatcgacttg aacaacgccc
     7801 gagccgtact cgccaccggc gtttcctcac tcgccgccgt ccgcgccgta accgaagcgg
     7861 caaatcccga agcggtggtt aaagcgtttc aggctttgtg ggatggataa aaccgaaaga
     7921 agaaaattca attgccgtgt aggcaaaact tagcccgtta tcgcaaacat acttaacttt
     7981 aaatgtggca tatcatcaaa ttccgtcatt cccgcgtaag cgggaatccg ctttaaaact
     8041 tgagaaacca tcatttgaaa aacagtttcc gaatttcaaa aatggattcc cgcctgcgcg
     8101 ggaatgacgg caactggccg gttgcgtatc aaaaaataaa gtaattc
//
LOCUS       DAWFJW010000086         8068 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFJW010000086 DAWFJW010000000
VERSION     DAWFJW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.086"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            167..1108
                     /gene="miaA"
                     /locus_tag="WGR19_001557"
     CDS             167..1108
                     /gene="miaA"
                     /locus_tag="WGR19_001557"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0799060.1"
                     /translation="MPIPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            1112..1831
                     /locus_tag="WGR19_001558"
     CDS             1112..1831
                     /locus_tag="WGR19_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ0799061.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1836..2324
                     /locus_tag="WGR19_001559"
     CDS             1836..2324
                     /locus_tag="WGR19_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0799062.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVHGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2391..3143
                     /gene="rlmB"
                     /locus_tag="WGR19_001560"
     CDS             2391..3143
                     /gene="rlmB"
                     /locus_tag="WGR19_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0799063.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGDADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3205..4596)
                     /locus_tag="WGR19_001561"
     CDS             complement(3205..4596)
                     /locus_tag="WGR19_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0799064.1"
                     /translation="MSGQLGKGADAPDLVYGLEDNPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGKEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            4902..5777
                     /locus_tag="WGR19_001562"
     CDS             4902..5777
                     /locus_tag="WGR19_001562"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0799065.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            5786..6982
                     /gene="bamC"
                     /locus_tag="WGR19_001563"
     CDS             5786..6982
                     /gene="bamC"
                     /locus_tag="WGR19_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0799066.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGASDIFFAHK
                     AMKEVYGDKNKDTTMWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(7039..7971)
                     /gene="pip"
                     /locus_tag="WGR19_001564"
     CDS             complement(7039..7971)
                     /gene="pip"
                     /locus_tag="WGR19_001564"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0799067.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGNRAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         2067 a         2643 c         1885 g         1473 t
ORIGIN      
        1 ttctctttga gctaaggcga ggcaacaccg tactggtttt tgttaatcca ctatataaag
       61 gcatacggta aacacgcccc cttgccgtcg gcgtgcttcc gcaaaccaaa atataacgtc
      121 ggcacgcttt ccgctataat ccgcattttc gcaaccctga aacatcatgc cgattcccaa
      181 agcctttgcc ctcttaggcc cgaccgcctg cggcaaaacc gcgctcgccc tcaaaattgc
      241 cgaaaccctg cctgtcgaaa tcatcagcct cgattccgcg ctagtctata ccggtatgga
      301 catcggcacg gcaaaaccgt ccgcctccga acgcgccttt gtcccgcacc accttatcga
      361 catcattacg cctgttcaga catacagtgc cgcccgtttt gtcgaagact gcacgcgtct
      421 gatcggcgaa atcacggcgc gtgggaaatg tccgctgatt gtcggcggca caatgatgta
      481 tttccgcgcc ctgacccaag gtttgaacga tttgcccgag gccgatgcct gcctgcgtgc
      541 cgacttggac gaacaaaaac agatgtacgg cttggatttc ctctaccgca ccctgcaaaa
      601 agtcgaccct gaaaccgcat gccgtctgaa accgaacgac agccagcgta tcgaacgcgc
      661 tttggaagtt tattatctga ccggcaaacc gatgagcgaa cacctcggca gacaaagccc
      721 ccatacgctc cccttcgatt tgcataccgc cgccctgatt cccgaaaacc gcgcccgcct
      781 gcatgaaaac atcgccctgc gtttccacct gatgcttgaa cagggcttta tcggcgaagt
      841 agaaaacctg cgccgccgct atcccggcct gaccgccgac tcccccgcca tccgctgcgt
      901 cggctaccgt caggcatggg aatatctgga cggaaaaacc gatttcccgg catttgtcga
      961 gaaaggcatt gccgccaccc gccaacttgc caaacgccaa ctgacttggt tgcgcaaaac
     1021 acctttagac tgcgttgccg acccgttttc agacggcact tcatgcacac gcctgattga
     1081 ggcggcaaaa cggtttttcg gcgcataaac catgctgacc acaccgccgc tcgcccccaa
     1141 aaccgtcgcc gccctgcaca ggctcggtat ccgtacgctc gaagaactgc ggcaaaacgg
     1201 ttcggtcaaa gcctttctgc tgctcaaagc atcgggtttg accctcacga aaagcacgct
     1261 gtggcagctt gagtcgctgc tgaacggcac actcccgcaa gaaatcacgc aacacgacaa
     1321 aacgcgcctt tttgccgaat tgaaaaacca tccgcccgtc gccgcctttc cgccgcagga
     1381 agaaatggaa cacttcatgc gcgaagcact gcgccaagca gaacaatccg ccgctgacgg
     1441 cgaaataccc gtcggggcag tcatcgtttc agacggcaaa atcatcgcat cggcacacaa
     1501 cacctgcatt gccgactgca atgtcagccg gcacgccgaa atcaacgcct tggcacaggc
     1561 aggcagcgaa atgcaaaact accgccttga cggatgcgac atatatatca ccctcgaacc
     1621 ctgcgccatg tgcgcgtccg cactgataca ggcacgaatc aggcgcgtga tctacggggc
     1681 ggcagaaccc aaaaccggcg cggcaggcag catcgtcaac ctgtttgccg acaaacgcct
     1741 caatacgcac accgccatac ggggcggaat cctgcaagaa gaatgccgcg ccgtgttaag
     1801 ccgttttttt caaaacaaaa gaaaaggttg aacctatgac cgtactgacc gtcatcctcg
     1861 ccctcgccct gatagccgtc ggcacggcgg gcatcgttta ccccgccctg cccggattgg
     1921 cattgatgtt tgccggaaca tggctgcttg cctatgccgg cggctaccaa atctacggcg
     1981 cgggcgtttt gtggacggtc ggactcatca gccttgccgg catactggcg gactatgtgg
     2041 caggcatatg ggggacaaaa tataccggag cgggcaagct cgccgttcac ggcgcattgg
     2101 ccggcagcat catcggcata tttttctccc ttcccggact aatactcggt ccctttatcg
     2161 gcgcggcggc aggcgaactg atcgaacggc gcaatatgct tcaggcaggt aaagcgggct
     2221 tgggtacgct gttggggctt gtcgtcggca cggcgttcaa aatcggctgc gccgtatcca
     2281 tcttgtttat cctgttggtg aaatacatcg cctacctgtt ttaacccttt cagacggcat
     2341 accggcaaat tgctataatg ctgtctgaac cctttttaca aagcaccatc atggcaaacc
     2401 aaagacccat ctacggcttc cacgccgtca acgcccgatt gtggcaaaac cccaaatcca
     2461 tcgtcgaact ctacatccaa gaaggcaaat cagacgcacg cacgcgcgaa gtgttggaaa
     2521 aggcggcaaa cgaaaacatc cgcgtatatt ttgccgatgc cgaccgcctc aacgccatca
     2581 gcaaaggcgc gcgccatcag ggcgtggtcg gatttatcga tgcctccaaa aaccacgtcc
     2641 acctcgaaga cgtattggaa aacctcagcg aaccgccgct gctgctgata ctcgacggca
     2701 tcaccgatcc gcacaacctc ggcgcgtgcc tgcgtaccgc cgacgcaatg ggcgtacacg
     2761 ccgtcatcgc accgaaagac aaaagcgcgg ggctgaacgc caccgtcagc aaagtcgcct
     2821 gcggtgcggc ggaaaccgtc ccctacatca ctgtaaccaa cctcgcccgc accctgcgcg
     2881 agctgaaaga atacggcatt tggatcatcg gcaccgatat gggcggcgat gccgaccttt
     2941 accattgcga tctgcccgac agcgcggcat gggtgatggg caacgaaggc gacggtatgc
     3001 gccgcctgac acgcgaacat tgcgacatgc tggtgtccat acccatgttc ggcacggtcg
     3061 aaagcatgaa cgtctccgtg tctgccggaa tggtattaag tgaaacacgc cgacaacgcg
     3121 tattaaaaaa cgaaaaagcg taagcctcaa aaacaatgcc gtctgaaaca gggattccgt
     3181 ttcagacggc attcattttc aaaatcagtg ttccaagtgg tcggtatcaa acttgaccgc
     3241 cgcctcggtt ttatcttcgg gcaagaccaa attcagcaag actgccgtaa tgccgccggc
     3301 ggaaatagag ttttggaaca agacgggcag gtttttaaac acttccggct caaacgcgac
     3361 acccaagccc aagccgaccg acgttgccgc aatcaccgcc tcgcgcctgc ggatgccatg
     3421 gctgactaaa atccgcacgc ccgcaatcgc aatcaagccg aacatcaaaa ccatcgcgcc
     3481 gcccaacacc ggactcggaa tcgtcgtaaa cgcgcgtccg acaacgggga acaaacccaa
     3541 cagcaccaaa atcacggcaa tatatttgcc cacatggcgc gaagccacgc cggtcatctg
     3601 aatcacgccg ttgttttgcg caaacgtcgt cagcggcagc gaacccaaag ccgtcgcaat
     3661 caccgacacc aagccgtcag ccaacacgcc gccgcgcagg cgtttggtgt attccttgcc
     3721 ttcaatcggc tggtcggaca ccattgccgt cgcggtcaaa tcgccgaccg cctcaaacac
     3781 gctcaacagg aaaatcgcgc ccgccacaat aaatgcgtgc cagtcgaaag caaaaccgta
     3841 tttaaacggt acgggcagcg taaccagcgg caggttttgc agtgccgaaa aatccacctt
     3901 gcccaaaaac agtgcgacga tatagccggc aatcagaccg accgcaatgc cgctcatccg
     3961 caacagcggg tttttcatgc agttgaatat caacacaatc agcaacacca gcgacgccag
     4021 ccccaggttt tccatcgaac cgaacgtgcc gtccgccttc gcgccgaagc cgccgccgaa
     4081 atcggtaata ccgacgtgta ccaaactcaa gccgatcagc atcaccacta cgccactgac
     4141 cgtcggcgta atcacttttt tcaaatacgg cagaagccac gccgagaaac acaccaaaaa
     4201 cgcgccgaca aacgatacgc ccaacagcgt cgaaatcatc gcatccttag tcaaaccgcc
     4261 ctccttcatc cccgcgccga gcgcaatcat gacggtaacg aacgagaaat tcaccgactg
     4321 gatggacagc atccccgaac cgaccggccc gaagcggttg acctgcaaat aagtgccgac
     4381 acccgacgca accatcgcca tcgacacgag atacgccgtc atttccaccg gcagttccag
     4441 cgcgccgccc acaatcagcg cgggcgtaat catcggcaca aaaatcgcca aaagatgggt
     4501 aacggcactc aagagcgcat tcccaaacgg cggattatct tccaaaccgt acaccaaatc
     4561 aggcgcatcc gcccctttgc ccaactgacc actcattgta tttccttatt ttaaaggttg
     4621 aaaaatccaa gcgcggattg tagcgaatcc gccataccgc ttcaatttcg atttcggacg
     4681 gcatacgcgc ccgtccggca aacagattcc gaaccgtcgc cgccggaata tcgtttacaa
     4741 aacgcaataa cctgatttca aacacaaaaa tacaaacccg acgcatccgc cgtcaagccg
     4801 ccccgctcat cccggacccg ccggatttca cacctgcggt aaagtaggct acaatcagaa
     4861 gcattaccgc cccctacacc acaactgata aaaggttctg tatgttacaa ggtagcttgg
     4921 ttgccctgat taccccgatg aatcaagacg gcagcatcca ttacgaacaa ctccgcgact
     4981 taatcgactg gcacattgaa aacggcacgg acggcatcgt tgccgtcggc acgacaggcg
     5041 aatccgccac cctctccgtc gaagaacaca ccgccgtcat cgaagccgtc gtcaaacacg
     5101 ttgccaaacg cgtccccgtc atcgccggca caggggcaaa caacaccgtc gaagccatcg
     5161 ccctttccca agccgccgaa aaagccgggg cggactacac cctctccgtc gtcccctatt
     5221 acaacaaacc ttcgcaagaa ggcatgtacc gccatttcaa agccgttgcc gaagccgccg
     5281 cgattccgat gatactctac aacgtacccg gcagaaccgt cgtcagcatg aacaacgaaa
     5341 ccatcctgcg ccttgccgaa atccccaaca tcgtcggcgt gaaagaagcc agcggcaaca
     5401 tcggcagcaa catcgaactc atcaaccgcg cgcccgaagg cttcgtcgtc ctttccggcg
     5461 acgaccatac cgccctgccg ttcatgctgt gcggcggaca cggcgtgatt accgtagccg
     5521 ccaacgccgc gcccaagctc tttgccgata tgtgccgcgc cgcgctgcaa gtcgacattg
     5581 ccctcgcacg cgaactcaac aaccggctga ttccgattta cgacaccatg ttctgcgaac
     5641 ccagccccgc cgcgccgaaa tgggcggtat ccgccttggg cagatgcgaa ccgcacgtcc
     5701 gcctgccgct cgttccgctg acggaaggcg gacaggcaaa agtccgcgcc gccctgaaag
     5761 cctcaggaca actctaaata taactatgcc gtctgaaccg ttcggacggc ataacgcaac
     5821 aaacacttta atatccatca cacaggatga cacgatgacc cttatcaaac ccgtcattgc
     5881 cgcgctcgca ctcatcgggc ttgccgcctg ctccggcagc aaaaccgaac agcccaagct
     5941 cgactaccaa agccggtcgc accgcctgat caaactcgaa gtcccgcccg atttgaacaa
     6001 tcccgaccaa ggcaacctct accgcctgcc tgccggttcg ggcgccgtcc gcgccagcga
     6061 tttggaaaaa cgccgcacac ccgccgtcca acagcctgcc gatgccgagg tattgaaaag
     6121 cgtcaaaggt gtccgcctcg agcgcgacgg cagccaacgc tggctcgttg tcgatggcaa
     6181 atcaccggcg gaaatatggc cgctcctgaa agccttctgg caggaaaacg gcttcgacat
     6241 caagtccgaa gaacctgcca tcggacaaat ggaaaccgag tgggcggaaa accgcgccaa
     6301 aatcccccaa gacagcttgc gccgcctatt cgacacagtc ggtttgggcg gcatctactc
     6361 caccggcgag cgcgacaaat tcatcgtccg tatcgaacag ggcaaaaacg gcgcttccga
     6421 catcttcttc gcccacaaag cgatgaaaga agtgtatggc gacaaaaaca aagacacgac
     6481 catgtggcag ccctccccgt ccgatcccaa tcttgaagcc gctttcctga cgcgctttat
     6541 gcaatatttg ggcgttgacg gacagcaggc ggaaaacgca tcggcaaaaa aacctaccct
     6601 tcccgccgcc aacgaaatgg cgcgtatcga aggcaaaagc ctgattgtct ttggcgacta
     6661 cggcagaaac tggcggcgca ccgcgctcgc cctcgaccgc atcgggctga ccgtcgtcgg
     6721 tcaaaacacc gaacgccacg ctttcctggt tcaaaaagcc ccgaacgaaa gcaatgcagt
     6781 taccgaacaa aaacccggcc tgttcaaacg cctgctgggc aaaggcaaag cggagaaacc
     6841 tgccgaacag ccggaactga ttgtctatgc cgagcctgtc gccaacggct cgcgcatcgt
     6901 cctgctcaac aaagacggca gcgcatatgc cggcaaagac gcatccgcat tattgggcaa
     6961 actccattcc gaactgcgtt aaaccattca aaacaaaagc agcttccaaa cagaagctgc
     7021 ttttttatgc ggaacttttt acaacaaatg gggcaaaata tcctcaaccg cctgaaccaa
     7081 cgcatccacc aaaggcggat cgaacgcacg atgccctgcc tgaaccaccc tcaattccgc
     7141 ttcgggaaag gctttcgaca gcgcccacgc actctgcatc ggcgtacaca aatcataccg
     7201 cccctgtacg ataacggtcg ggatatgccg tattttgccg atattgttca aaatcgccct
     7261 attgccctgc aaccaaccgc cgttgacaaa ataatggttt tccaaacgcg cgattgccag
     7321 cgaggcataa gcatcttcat ccacttcctc cggctcgaaa cggatcagat agctttccca
     7381 atccgcccaa gcctttgcgg cagacaggca cacttcttca tcttgatgaa acagcaatcc
     7441 gtgatacgcc tcaatcagcc ggttccgccg attttcagca atcggcgcga caaatttttg
     7501 ccattgttcc ggataaatcc ggctcacacc gcccgcctcg ttcagccacg ccgtttcaga
     7561 cggcctgcac aaaaatatcc cgcgcaacac caatcccttt acccgttccg gatgggtttg
     7621 ggcataagcc agcgacaaag tgctgcccca cgaaccgccg aacaccagcc atttcccgat
     7681 acccagcatt tcacggactt tttcaatatc tgccaccaaa tcccaagtcg tattgtcttc
     7741 cgcacaagca tacgggcgcg aacgtccgca accgcgctgg tcgatgatga cgatgcggaa
     7801 cacatcggga ttgaaaaaac cccgacattc aggcgaagcc cccgcgcccg gcccaccatg
     7861 taaaaaaatg accggcacac cgtcgggatt acccgactct tcccaataaa tttgatgaat
     7921 ttcagacacc tgcaagtatc cgctatgaaa aggctgtttt atctcataca tcatcttctc
     7981 ccatattgct tctgacaatc cgaacattcc cacacgcaac atcacgcacg agacacaagc
     8041 cgccccgaaa ggcaaatgcc gaaaaatc
//
LOCUS       DAWFJW010000087         7990 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFJW010000087 DAWFJW010000000
VERSION     DAWFJW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.087"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(159..1859)
                     /gene="recJ"
                     /locus_tag="WGR19_001565"
     CDS             complement(159..1859)
                     /gene="recJ"
                     /locus_tag="WGR19_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0799068.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERQEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLTALRAELRRRDYFSDGIKEP
                     NLGDLLDLVALGTVADVVTLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIDCLLARDDVEAQELAAQLNNLNIERREIEQSM
                     LQDALSDFPKTLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFFRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDVLDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGYEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(1928..2644)
                     /locus_tag="WGR19_001566"
     CDS             complement(1928..2644)
                     /locus_tag="WGR19_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799069.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTDGLCLRYDTDRPDDIAKLKQLEFEAVELDKQTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPVEIYYMVKGEYTDKLKLFGNILYTPPLLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(2691..3197)
                     /locus_tag="WGR19_001567"
     CDS             complement(2691..3197)
                     /locus_tag="WGR19_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0799070.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            3352..4683
                     /gene="pmbA"
                     /locus_tag="WGR19_001568"
     CDS             3352..4683
                     /gene="pmbA"
                     /locus_tag="WGR19_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0799071.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(4980..5183)
                     /locus_tag="WGR19_001569"
     CDS             complement(4980..5183)
                     /locus_tag="WGR19_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0799072.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            5426..5728
                     /gene="clpS"
                     /locus_tag="WGR19_001570"
     CDS             5426..5728
                     /gene="clpS"
                     /locus_tag="WGR19_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0799073.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            5730..>7990
                     /gene="clpA"
                     /locus_tag="WGR19_001571"
     CDS             5730..>7990
                     /gene="clpA"
                     /locus_tag="WGR19_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0799074.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVHNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNSDIPDALKDTEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPVLAKGSLRCIGATTYDEYRTIFDK
                     DHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKKVEAE
                     FTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDWDTAK
                     EEAVLKFKKSKVK"
BASE COUNT         1908 a         2349 c         2026 g         1707 t
ORIGIN      
        1 agtctgaaat ttgatgcacc ttatatattg cgtccctaag aaaggatgat taacaaaaat
       61 taacgccctt tactttcttt tccgattggg aacattcccg tcgtactaac aggcaaatga
      121 aggaacagaa atcacattca aacggcgttc cgccgcctct acaccgcttc ccagtagtcg
      181 atataaagct gcaattcgag attattccgc cattcgttgg caacggggcg gtacaccgtg
      241 cggatgtatt cgggaatgtc ttcgctacaa cgccaaaaca tggcttcaaa ttcgtagccg
      301 tctttttgca gccagacttt tttatgtttg ccttccgcac ccaaaggctg ctggcggaca
      361 acgtgaaatt cgtcggtaaa actcggcggc gcgaagccct gcccccaaac gtggcgggcg
      421 aggttttgcg cctgttccaa cgtgatgtcg caggctttca ggctgccgtc ggtaatatag
      481 gtctgcgaca aatcgtcttc gcacaccatc tcgcgcacgg cttcttcaaa ggctgtctga
      541 aacgcgggaa tattgtgttc gagtatgctc aaacccgccg ccattgcgtg tccgccgaat
      601 ttcaaaatca aatcgggatg gcgtttggac accaagtcca aaacatcgcg caggtgcaaa
      661 ttgggaatgg agcgtcccga accacgcact tcgccgttgt cggcaggcgc aaacacgatg
      721 gtcggacgaa aaaaacggtc tttgagacga ctggcaacga taccgaccac gccttgatgg
      781 aagtcgtcgc gatacgccac caaagtcgtc tgacctgaag gcagggtttt gggaaaatca
      841 ctcagcgcgt cttgcagcat ggattgctcg atttcgcggc gttcgatatt gaggttgttt
      901 aactgggccg ccagttcctg cgcttccaca tcatctcgcg ccaacagaca gtcaatgccg
      961 accgacatat cgtccagccg tccggcggcg ttgatgcgcg gtcccaacgc aaagcccata
     1021 tcaaacggct gagccttgcg ccaatcccgt cgtgctactt caaacaaggc gcggatgccg
     1081 gggcgcattt tgcccgaacg catccgtttc aaaccttgcg acacgaggat acggttgttg
     1141 tggtctagag taacgacatc ggcgacagtg ccgagtgcga ccaaatccaa aaggtcgccc
     1201 agattcggct ctttgatgcc gtctgaaaaa taatcgcggc ggcgcagttc ggcacgcaac
     1261 gccgtcaata cataaaaaat cacgcccacg cccgccaaac ttttgctttg gaagccgcag
     1321 cctttttggt tcggattgac gatgatgcag tcgggcacgg tctcggcagg cagatggtgg
     1381 tcggtaacga tgacatccaa acccagagcc tgcgcgcgcg ccacgcctgc gatgctggca
     1441 atgccgttat cgaccgtaat cagcaaatct acaccttgct ctgccgcgat ttcagccagc
     1501 tcgggcgtta aaccgtagcc gtgttcaaag cggttgggca caaggaaatc cactttcgcc
     1561 cccatcgccg ccaaaccgct cataccgacg gaacacgccg tcgcgccgtc ggcatcatag
     1621 tcggcgacaa tcaggatttt ttcctgacgc tcaaccgcat ccgccaaacg gccggcggcg
     1681 gcttcgcaat tcgtcagcga ttgataaggc aggagggaag cgagtttgtc gtccaattcg
     1741 gcaggacttt gcacaccgcg cgaagcacaa agccgggcga ttaaaggatc ggcaccggcg
     1801 gtgagcaaat gattaaaaac gtcggtattg atggatcggg tttggatttt gactgacata
     1861 acgtggggtt ttactgtgcg tgtgtgctta aaatgccgtc tgaaagcttt tcagacggca
     1921 ttgctgttca tttgcgggcg gcatccacga ccgcacccag agccgccgca ggcaaggcca
     1981 gtaccgcgcc cgccgcatcc agtatcaaca aggggggcgt atataagata tttccaaaca
     2041 gcttgagttt gtcggtatat tctcccttaa ccatataata aatctcgaca ggcacacttt
     2101 gctcaaaatg gtaatcggcg ttcagttttt gcggtgtggc gtagtatttg cctttggcag
     2161 agacgcagcg cgtgtaaata gtctgtttgt cgagttcgac cgcttcaaac tcaagctgtt
     2221 tcagcttggc gatgtcgtca ggcctgtcgg tatcgtagcg caggcaaagg ccgtcggtac
     2281 tgaaattctg gctgccgggc gattcgagtt tgaccggcag ggcttggtgg cgggcatagc
     2341 tcggggtatc ctcaactatt tggaagggct tgtccagccc ggccttcaaa atgcccgtca
     2401 gcttcgccga atcttcagga ttgacgacga accagtattt tccgcccatc atcaccaggc
     2461 tgcccttttc caattgggca ttgtcttcgg caaccacacc gaaggcgcgg atttggtctt
     2521 tgtcaacgtg tttgcgggtg attgtttcgc tgaccgggtt gttcattccc cacaacatca
     2581 acgtacagcc gttcagcatc aaggttgccg ccaaaacggc ggcggtcgtt ttacggaaca
     2641 acattttatc ctccaatcta aataacggcg gatgtcgtct gaaaatatgc ctaaatttcc
     2701 gcgtccccgt tttccatcac ggttttcaac tcttgaaaca gggcgcggaa attttttggt
     2761 ggtttgtttt gctcctgctc ttttttggta ttgcggatga gtgtcctcag cttgcccgcg
     2821 tccgcatgcg gaaaatccga cataaactgc gtcaacgcgc cgtcgtctgc cagcagctcc
     2881 atccgcgtcc gctccacacg ttgcaaaaag gcgttgtgcg ccgcatcgtc gccgcgcagc
     2941 ttggcaagaa atgcctcgat gggcgcggga tcggtatcgc gcatcagtct gccgataaat
     3001 tgcgcctgcc gcttgagcgc gccgttggat gtgatttttt tataggtaac gacggcttcg
     3061 tacaaatctt cgtccaagcc gatttttttc aacgtgtcgc ttgagagctt ggtcagctcc
     3121 atacccaaat cctgcaaacc gttcatctgc tttttcattt gggttttgct gacccattcg
     3181 tcttcttgtt caaacatctt cattctcaaa ttttatccga cgggcgcatt ttaacagaat
     3241 gattccgcgt tcagacggca ttttgcaccg tccgccctgc aaacggcacg cttttgcccc
     3301 tgccgcccga aacggttaaa atgccgcaac gctccaccaa gaaagatttc tatgctgttc
     3361 aaccacaccg cttccgaact tctcgacctc tgccgccgca cgctcgactt ggcaaaagca
     3421 gcgggcgcga ccgccgccga agccgatttc agcgaatcca tcggacaaag cgtcagcgtg
     3481 cggctgggcg aaatcgaaca aatcgagttc cagcaggaca agtcgctgga cattaccgtt
     3541 tacgtcggca aacgcaaagg ccgcgctggc accgccgatt tctccgaaca agccctgcgc
     3601 gacaccgtcc gcgccgccgc cgacatcgcc cgccacaccg ccgaagacgg ttgcgccgga
     3661 cttgccgacc ccgagttgat ggcgcaacac atcggcgacc ccgaccttta ccacgaatgg
     3721 gatttggata cggaagccgc cgtcggcttt gcaaaacaat gcgaacaagc cgccctgaac
     3781 gaggatgagc gcatcgaaaa ctccgaaggc gcggcggtgc aaaccggcca ttaccaatac
     3841 gtttacggca acacccacgg ttttgccgca caccggcaag gcacgcacca cagcatttcc
     3901 tgcagcgtcg ttgccgccga cgaaaacggg atgcagcgcg actactggta cgattccgcc
     3961 tgccgccatc cggatatgga cagccccgaa accatcggtc aaaccgccgc ccgccgcact
     4021 ttgcgccgac tcggcagccg cagcatcccg acaggcagct accccgtcct cttcgatacc
     4081 accgtttcgg gcggccttat cggacacctc gtcggcgcac tctccggcgg cgcgctctac
     4141 cgccaaagca gtttcctgat cgacagcatc ggcaaaaaag tcctgcccga tttcctcaac
     4201 ctgcgcgaag aaccgcacat cccccgctct ttccgcagca gctattttga tgcggaaggc
     4261 gttgccaccg caccgcgctt cgtgattcaa aacggcattg tcgaaggcta tttcctcagc
     4321 agttacagcg cgcgcaaact cggtatgcag accacgggca acgccggcgg cgcgcacaac
     4381 ctgtatttga accatacgca cgaaacgcaa tccgacctgc tgaaagaaat ggggacggga
     4441 ttattggtta ccgaactgat gggacagggc gcgaacacca ttaccggtga ctactcgcgc
     4501 ggcgcggcgg gtttttgggt ggaaaacggc gtgattgcct accccgtcca cgagattacc
     4561 gtagccggac gcttgcagga tatgtaccgc gacattgtcg gcgtggcgga tgacgctttg
     4621 cggcgttcgt ccaacaaaat cggctcgatt ctgattgcgg ggatgacggt tgccggaagc
     4681 tgaacgccct gccgatgccg tctgaagacg aaggcagcac gttttgcccc gtgcctgccc
     4741 gtttgccgtt ttatgccgca ccgccttttg ccgacaacat tgcacatttt ttcatcagca
     4801 ctgattttcc cttatccgtc aaaatactaa gccacttgtc cacaacaggg tacacacaag
     4861 ttcaggataa ggcgtgcggg gattggggtt tttccgccgc cggcaaaacg gcagacaaaa
     4921 acggcgggac attgcctgtc ccgccgtttt caaatataca tttgaccgta catcaggaat
     4981 taagcagcct gaatgttggc ggcctgtttg cctttagggc cggtggttac gtcgaaagag
     5041 acgcgttggc cttctttcag ggttttgaaa ccttccatat tgattgctga gaagtgagcg
     5101 aacaaatctt cgccgccttc atcaggcgtg atgaaaccaa aacctttagc gtcgttaaac
     5161 cattttacga taccggttgc cattagaaac ttcctatact caaaaattaa caaaatcagc
     5221 aaaacaaggc atacggcaaa ccgagccgtc tggcgttcct tccagcttga tatactcggc
     5281 ggatgcccaa tcctgcttaa ccgtcttttt acatttgtta agacaaaacg tcaagctatg
     5341 ttttcaaaaa agtgggaaaa taggcaaaag taccgaaaat acaacaaaaa tgccgtctga
     5401 acgcggcttt caaatgggac acactatgac cgcgcaacac caatccgaca cgctcttgca
     5461 ccgtctgaac accctgccgc cgaaacgtta cggcgttttc ctattgaacg acgattacac
     5521 cacgatggaa tttgtcgtcg aaatcctgac cgaaatcttt atgctcggac aagaacaggc
     5581 ggtagcggta atgctcttgg ttcatcacga aggcaaaggc ctgtgcggca cttacacgcg
     5641 cgatattgcc caaaccaaac aacaacaagt catgcagcgg gcaaaagccg aagggcatcc
     5701 gctgcaatgt attgtcgagg agatttaata tgcttgcacc cgaattggaa cagattttgc
     5761 agcagcttta ccgcgaggcg cgtaaggctc attatgaatt tatcagcctc gagcatctgc
     5821 ttttggtact catcgaagaa gatgcctccg tccacaacgt cctcaagctc tgcggcgcgg
     5881 atttgaaagt ggtgtccgaa cagctcgccg ccagcgttgc cgaaaacacc cccctgattc
     5941 ccgaacacct tttagacacg gtcgaaaccc ggcccacgct cggcttccaa cgcgtgatgc
     6001 aacgggcgat ggtgcatacc cagtctgccg gaaaagccgc agtcgaaccg ttggacgttt
     6061 tggtcgcgct gatgagcgaa accgacagcc acaccgtcta tttcctcaag ctgcaatcgg
     6121 ttacgcgttt tgaagttttg cgctgtattg cccacggctc tcccgatgac gaggatggca
     6181 atgattcaga cggcctggga cgcgagggcg aagaagcgga acaaaaaacc ggctcccttt
     6241 ccgactacac cgtcaacctc aacgccgaag tcaaagccgg ccgtatcgac cctttgattg
     6301 gtcgcaaaca cgaaatggaa cggctggtgc aaatcctgtg ccgccgccgc aaaaacaatc
     6361 cgcttttggt cggcgaagca ggtgtgggca aaaccgcgct ggcggaaggt ttggcacatc
     6421 aaatcgtcaa cagcgacatt cccgacgcgc tcaaagatac cgaagtgtac gcgctggata
     6481 tgggttcgct gttggcgggc acgaaatacc gcggcgactt tgaagcgcgg gtcaaatccg
     6541 tcttgaaaca gctcgaaaaa atcccgcacg ccattttgtt tatcgacgaa atccacacca
     6601 tcatcggcgc gggcagcacc agcggcggca ccatggacgc gtccaacctg ctcaaacccg
     6661 tgctggcaaa aggttcgctg cgctgcatcg gcgcgaccac ctacgacgaa taccgcacca
     6721 ttttcgacaa agaccatgcc ttaagccgcc gcttccaaaa aatcgacgtg gtcgaaccca
     6781 ccgtttccga aaccgttcaa atcctgcgcg gcttgaaacc gatgtttgaa gccttccacc
     6841 aagtccgcta cactcaaggc gcactcgaag ccgccgccga actctccgca cgctacatca
     6901 acgagcgttt cctgcccgac aaagccatcg acgtaatgga tgaagcaggc gcggcgcaac
     6961 ggattctgcc caaatccaaa cagaaaaaag tcatcggcaa agcacaaatc gaaaccgtca
     7021 tcgccaaagt ttcgcggatt cccgaaaaaa ccgtgtcgca cgacgacaaa caagtgctgc
     7081 aattcctcgg tcgcgatttg aaaaacatgg tttacggtca agaaaacgcc atcgacgcgc
     7141 tggttgccgc cgtcaaaatg tcgcgttccg gccttgccct gcccgacaaa ccgataggca
     7201 gcttcctctt ctccggcccg accggcgtgg gcaaaaccga agccgccaaa cagcttgcct
     7261 actcgctggg cgtaccgctg caacgctttg atatgtccga atacatggaa cgccatgccg
     7321 tatcgcgcct catcggcgcg ccaccgggct acgtcggctt tgaacaaggc ggccttttga
     7381 ccgaagccgt caacaaacag ccgcattgcg tattgctctt ggacgaaatc gaaaaagccc
     7441 accccgacat tttcaacgtc ctcctgcaag tcatggacgc aggcaaactg accgacaaca
     7501 acggcaagag tgccgatttc cgcaacgtca tcctgattat gaccaccaac gcaggcgcgg
     7561 aaagcctcag ccgccccagc ctcggcttta ccgccaagcg cgaacgcggc gacgaaatgc
     7621 aggcaatcaa caagctcttc acgcccgagt tccgcaaccg cttggacgcg attatcccgt
     7681 ttgcgccctt gtccgaaccc atcatcgtca aagtcgtgga caaattcctg ctccagctcg
     7741 aacaccagct cctcgacaaa aaagtcgaag ccgaattcac gtcggcattg cacaaatatc
     7801 tggcggaaaa aggtttcgac ccgcaaatgg gcgcgcgccc gatgcaccgc ctgattcagg
     7861 aaaaaatccg caaaccgctc gccgacgaac tcctgttcgg caaactcgcc gacggcggct
     7921 ttgtgcggat agactgggat acggcaaaag aagaagccgt gttgaagttt aagaaaagca
     7981 aggtcaaaat
//
LOCUS       DAWFJW010000088         7980 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFJW010000088 DAWFJW010000000
VERSION     DAWFJW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.088"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(66..425)
                     /gene="rnpB"
                     /locus_tag="WGR19_001572"
     ncRNA           complement(66..425)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGR19_001572"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WGR19_001573"
     CDS             complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WGR19_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0799075.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1710..2603)
                     /locus_tag="WGR19_001574"
     CDS             complement(1710..2603)
                     /locus_tag="WGR19_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0799076.1"
                     /translation="MVTFSKIRPLLAIAAAVLLAACGTAGNNAARKQVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2945..3394
                     /locus_tag="WGR19_001575"
     CDS             2945..3394
                     /locus_tag="WGR19_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0799077.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3468..3776
                     /locus_tag="WGR19_001576"
     CDS             3468..3776
                     /locus_tag="WGR19_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799078.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3892..4734
                     /locus_tag="WGR19_001577"
     CDS             3892..4734
                     /locus_tag="WGR19_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0799079.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4724..5470
                     /locus_tag="WGR19_001578"
     CDS             4724..5470
                     /locus_tag="WGR19_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0799080.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5492..6079
                     /locus_tag="WGR19_001579"
     CDS             5492..6079
                     /locus_tag="WGR19_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0799081.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6085..6264
                     /locus_tag="WGR19_001580"
     CDS             6085..6264
                     /locus_tag="WGR19_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0799082.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            6496..6936
                     /locus_tag="WGR19_001581"
     CDS             6496..6936
                     /locus_tag="WGR19_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799083.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(7465..7821)
                     /locus_tag="WGR19_001582"
                     /pseudo
     CDS             complement(7465..7821)
                     /locus_tag="WGR19_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7836..7980
                     /locus_tag="WGR19_001583"
                     /pseudo
     CDS             7836..7980
                     /locus_tag="WGR19_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2110 a         2089 c         1773 g         2008 t
ORIGIN      
        1 caacctaaat aaaaatgccg tctgaaccat atttcaggtt tcagacggca tttgcgtgtc
       61 ggatgcacac cggacaggcg gtaagccggg ttctgtctcg gacagtcatt cctctaggca
      121 taccgttacc ggtatgctca agcaacctac ccgaacgctc ggcgggcagc gtcattgcgt
      181 tctgtttggt cttgctccga atggggtttg gcctgccgca tattgttacc aaatgcgcgg
      241 tgcgccctta ccgcaccttt tcacccttgc ctgtgctgcc aaagcagcca tcggcggttt
      301 tgctttctgt tccactttcc gtcgcgttac cgcgcccggc cgttaaccgg cattctaccc
      361 tgcggagccc ggactttcct ccccgtatgc cttacgcgat acgcggcgac tgtctgcccg
      421 tcccgtgtgc ggcgcggatt ataacacgaa acgcaaaaat gccgtctgaa acggtacagg
      481 tttcagacgg catacagcct aaactacacg ccctgtttca ggctggcttc gataaagccg
      541 tccaaatcgc catccaatac ggctttggtg ttgccgactt cgtagcctgt acgcaagtct
      601 ttgatacgtg aggaatccaa aacatacgaa cggatttggc tgccccaacc tacatcggat
      661 ttaccttctt ccaacgcctg tttctcttca ttgcgtttgc gcatttccaa ttcatacagt
      721 ttggacttca acatttccat cgcggcggct ttgttggcgt gttgcgaacg gtcgttttga
      781 cattgcacca caatccccgt cggctcgtgg gtaatgcgca cggcggagtc ggttttattg
      841 atgtgctgac cgcccgcgcc cgatgcgcga taggtgtcga tacgcaaatc ggcggggttg
      901 atttcgattt caatggaatc atcgatttca gggtaaacga acacggaggc aaacgaagta
      961 tggcgtttgt tgttcgagtc aaacggtgaa tagcgcacca agcggtgaac gccggtttcg
     1021 gtacgcagca aaccataggc gtattcgcct tccactcgga tagtcgcgcg gttgatgccc
     1081 gcgatttcgc cgtcgtcttc ttcgaggatt tcgattttga agcctttgcg ctcggcgtag
     1141 cggctgtaca tacggaacag catacccgcc cagtcttccg cttccgtacc gcccgcgcct
     1201 gcggtgatgt cgataaagca gttgttcggg tcggcgggct ggttgaacat ccgtttgaac
     1261 tccaaatccg ccatctgttt ttccagcccc gccacatctt cctgcacggc ggcaaaacct
     1321 tcttcgtcgt tttcttcgac ggtcatttca atcagcatgc ggttgtcttc gatgcccgaa
     1381 gcgatgttgt cgagcgtcaa cacgatgcct tcgaggattt tgcgctcttt gccgatttct
     1441 tgggcgcgtt tcgggtcgtt ccaaagttcg gggtcttcgg aaagaccgat aacttcttcc
     1501 aatcggtctt tcttaccctg ataatccata taaactcgga tgtcttcgct gcgcttttcc
     1561 aaatcattca gggtattgtt gagctggttg attacttcgg cttccatgat tcttttgttc
     1621 tttcaaaatt ttaggggcgt attgtacggg attcgggtat ttttttctat ggataaagcc
     1681 ttctagaaac acgttcggac ggcatagcgt caataacggt atgccgccag tttgcgtttg
     1741 atttcaggca atgcggcacg tgctgcctcc tcacccaact ggatggcacg ttttttctga
     1801 tcgaatccgc cgactgcacc caaatccaaa acctgcggtt tgataaccac atccgcctgc
     1861 cccaactcat tttgcaacgc ggaaacgctc attacgttca gcgtctgatc gagataagag
     1921 aagaagcctt ggctgatgtt tttgcctgga cgggcggaaa tatcgacggc aatcacgaaa
     1981 ttcgccccct gccgccgggc ggcactgacg ggcacgggct gcgacagacc gccgtcaaca
     2041 tatgtatgcc tgccgatgat aacgggttgg aacacattgg gaatggcggc ggaagcgcgc
     2101 acagcctgcc cggcattccc ctgattgaaa gcgacggcct tgccggtttc aaaatcagta
     2161 gcaacggcgg caaatttgat gggaaactgc tgaatctgcc tgccgccgac ttttcggttg
     2221 atgtaatttt gcagcttttc gcctttgata aaaccactgg tggacaaggt taaatcgacc
     2281 aaatcggttt tgcctaaaat ttcggcttcc aattcgaggc ggtcgggcga catacccgat
     2341 gcaaaaaggc tgccgacaat cgaacctgcc gatgtgccgg taaccacctt cacaggaata
     2401 ccgttttctt tcaaaacctt aataatgcct acatgggcaa atcctttaga cgcgccgcca
     2461 ccgagagcca aaccgaccac tgcggcgggt ttggcggttt gcacctgctt gcgggcggca
     2521 ttgtttcccg ccgtaccgca ggcggcaagc aacacggcgg cggcgattgc caaaagcggt
     2581 ctgatttttg aaaacgttac catattttcc attcctttat atatcgcacc ccgtcaaaaa
     2641 gagggattgc ttttcttaac aaccccctct gacagccaag caaatggggg ctttgttaag
     2701 tcatcatcaa aattaatatt tctttttttc ctttacggaa attatatttg aaggcatact
     2761 atccaaggcg ggaattatct cacaacaccg ccgttatcca aatatcccgc ctttttccct
     2821 ttctttccat caaaatactt tctttttata ttcattaact tgttaaatca ttggctgccg
     2881 ggtgtcagtt tttccgacaa aatccgtcta atggggtatc aacagaacca aaacaggaac
     2941 acttatgaaa atcggaacaa cttggcagac ggcatccgct atgctggttt tgcgtctgtt
     3001 tgccgcatat gaatttttgg aatcgggttt gcaaaaatgg aacggggaga attggttttc
     3061 tgaaatcaac gatcaattcc cattcccgtt caacttgctg ccggacgcgt taaactggaa
     3121 tctcgctatg tatgcggagc ttttactgcc cgtactgttg cttttgggtt tggcaacgcg
     3181 tctgtcggca ttagggctga tggtcgttac cgccgtcgct tgggctgcgg ttcacgccgg
     3241 ttcgggttac aatgtctgcg acaacggtta taaaatggct ttaatttata tcgtggtatt
     3301 aatcccgctg cttttccagg gtgcgggcgg atggtcgctg gatacgctgc tgaaaaaacg
     3361 gttttgcccc caatgccgtc tgaagcaaga ttgattcagt cgtggaatct gactttaaac
     3421 attccaacct tatctcgtta acttgatatt ctgaaaagga aatagaaatg aacaaaaaca
     3481 ttgctgccgc actcgccggt gctttatccc tgtctttggc cgccggtgca gttgctgcca
     3541 acaaaccggc aagcaacgca acaggcgttc ataaatccgc ccatggctct tgcggcgcgt
     3601 ccaaatctgc cgaaggttcg tgcggcgcgg ctggttctaa agcaggcgaa ggcaaatgcg
     3661 gcgagggcaa atgcggtgcg accgtaaaaa aaacccacaa acacaccaaa gcatctaaag
     3721 ccaaggccaa atctgccgaa ggcaaatgcg gcgaaggcaa atgcggttct aaataatccc
     3781 accccttcaa accaagccgc gtttttcagt aaaatgcggc ttttttaacg gcaaacaaag
     3841 attttttaac aagcacatca ttcttttgtg ccatccgaac cgggtaaaaa tatgattcaa
     3901 cacgcaggct tgggctaccg ccgcgacttg gcggaagact ttctctcgct ttccgaaaac
     3961 agcccgatat gctttatcga agccgcaccg gaaaactggc tgaaaatggg cggctgggca
     4021 cgcaaacagt ttgaccgtgt ggcggaacgg ctgccgctgg cgttgcacgg attgtctatg
     4081 tcgctgggcg ggcaagcacc gctggatact gatttgatag acggcatcaa agaaatgatg
     4141 cgccgttacg attgcacgtt tttctccgac catttgagct actgccacga cggcggtcat
     4201 ctttacgatt tgttgccgct gccctttacc gaggaaatgg tgcatcatac ggcgcggcgt
     4261 atccgcgaag tgcaagaccg tttgggctgc cgcatcgccg tggaaaacac gtcctactat
     4321 ctgcattccc cgcttgccga gatgaacgag gtcgagttcc tcaacgcggt tgcacgtgag
     4381 gccgattgcg gcattcatct ggacgtgaac aatatctacg tcaacgccgt caatcacggc
     4441 ctgttgtcgc cggaggcttt tttgaaaaac gttgacgcag atcgcgtgtg ctatatccat
     4501 attgccggac atgacgtgga aacgccggaa ttgttgattg atacacatgg cgcggcagtt
     4561 ttgccgactg tttgggactt gctcgaactt gcctatgcca agctgccgac gattccgccc
     4621 accctattgg aacgcgattt taatttcccg ccttttgccg aactcgaagc cgaggtcgcc
     4681 aaaattgccg actatcaaac gcgtgccgga aaggaatacc gccgtgcagc ctgaaatctc
     4741 cgcccaatac caacaccgtt tcgcccaagc catacgcgag ggtgaagccg ccgacggact
     4801 gccgcaagag cgcctgaacg tctatatccg cctgatacgc aacaatatcc acagctttat
     4861 cgaccgctgc tataccgaaa cgcggcaata ctttgacagc gaagaatgga gccgtctgaa
     4921 agaaggtttc gtccgcgacg cgcacgccca aacgccctat tttcaagaaa tccccggcga
     4981 gttcctacaa tattgccaaa gcctgccgct ttcagacggc attttggcac tgatggattt
     5041 tgaatatacc caattgctgg cagaagttgc tcaaattccg gatattcccg acattcatta
     5101 ttcaaatgac agcaaataca caccctcccc tgcggccttt atccggcaat atcgatatga
     5161 tgttaccgat gatttgcatg aagcggaaac agccttgtta atatggcgaa acgccgaaga
     5221 tgatgtgatg taccaaacat tggacggctt cgatatgatg ctgctggaga taatgggttc
     5281 ttccgcgctt tcgtttgaca ccctcgccca aacccttgtc gaatttatgc ctgaggacga
     5341 taattggaaa aatattttgc ttgggaaatg gtcaggctgg actgaacaaa ggattatcat
     5401 cccctccttg tccgccatat ccgaaaatat ggaaggcaat tccccgggcc aaaaccatct
     5461 atccgcataa aattaccttg ttcccgatac tatgccgcta cccgacctga ccgatgccga
     5521 attaatagag tcgcgtaaac tgcttctgca ttttgcgcgg cttcagttgc ccgaccaccc
     5581 tgatttggct gaagatttag tgcaggaaac attgctgtcc gcatacagcg caggcgacag
     5641 ttttcaaggc agggcacttg tcaacagctg gctttttgcc atattgaaaa acaaaattat
     5701 tgacgcatta cgtcaaatcg gaaggcagag gaaagtcttt accgcactgg atgacgagct
     5761 actggatgaa gcatttgaaa gccatttttc ccaaaacggg cattggacgc cggaagggca
     5821 gccgcaacat tggaacactc cggaaaaatc attaaacaac aacgaattcc aaaaaattct
     5881 gcaaagctgc ctatacaacc tgcctgaaaa caccgcacgg gtatttaccc tgaaggaaat
     5941 actcggtttt tcatccgacg aaatacaaca aatgtgcggt atcagcacgt ccaactacca
     6001 caccattatg caccgcgccc gagaatcatt gcgccaatgc ctgcaaatca aatggttcaa
     6061 ccaagaaaac ccgaagtaaa cgttatgaaa aaatgccgcg atatcgccct gcttctttcc
     6121 aaacatcagg accgggaaac caccccgggc gagaagattt ccatatacac acacctgctg
     6181 ttctgtccgt attgccgtga atataaaaga cagcttcaaa ccatcaaaat atcactggca
     6241 aaaacaacca gaacttcaaa ataaatgccg tctgaaaagg cttcagacgg cataagctga
     6301 cggaaacaaa tcaaaccgat ttactgttat cggcagttca tccataatac acgcttcaaa
     6361 agcagcatat tcctccatac ggaatatata aatacgcaaa acacgaaggc tgcatcaatt
     6421 tgctttattt gccttatttc acagacggcg ctacccctcc cgcccaaccc gttctttctg
     6481 aatgagcaga tttcaatgat taaggaaacc ctaatgcgcc caatcttcct atctttcgtt
     6541 ttactcccta ttttgataac cgcctgcagc acaccggaca agtctgcccg atgggaaaat
     6601 atcggcacaa tctcaaacgg caatattcat acatatatca ataaagacag cgtgagaaaa
     6661 aacggaaatc tgatgatttt ccaagataaa aaagttgtta ccaatctaaa acaagaacgt
     6721 tttgccaaca cccccgcata caagactgcc attgccgagt gggaaatcca ctgcaacaac
     6781 aaaacatacc gcttaagttc gctacagttg tttgatacaa aaaacacgga aatttccaca
     6841 caaagctaca cagcctcttc cctccgcccg atgagcatcc tgtccgggac attaaccgaa
     6901 aaacaatatg aaaccgtatg cggaaaaaaa ctctgattgc aacttatata caaacttacc
     6961 cacaaacctt atcataaaaa tgccgtctga aatactgaaa tatcagcatt tcagacggca
     7021 ttttgccatt ccctgaaaat tatccacaaa gttatccaca ttatttttta aaaccggctt
     7081 ccatccgaaa tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
     7141 cagatagtac ggcaaggcga ggcaacgccg tactggttta aagttaatcc actatataaa
     7201 ctcgctatac aatttcacta tccaaacgta aattgttcca ttgatacaca aaacttctta
     7261 cccccataat tttgataaag catttcttac attcccggct ccgtcccgta accaacacag
     7321 cctctaaaaa ataatcgctt gttttgacac cgatacactc atatagtgga ttaacaaaaa
     7381 tcaggacaag gcgacgaagc cgcagacagt acagatagta cggcaaggcg aggcaacgct
     7441 gtactggttt aaatttaatc cactatacaa atacagaaac tcaagaaaat aaccttgtgt
     7501 attgaccatc tcaagcaatt cagaaaaatc aagaaatttt ctgaccgtaa acaaacgttt
     7561 ccctaaaaaa acgatgtctt caaaaatatc gaccaaataa tcatcttcgc cgttttcatt
     7621 tttatgggtt aaaacaacac gggaaaaatc tgttttcaga tgcttgcccg cttgattgtt
     7681 cggattattg tccggaacga caaaaccgtc ctcaaaatca aagtagacgt tgcgtccttc
     7741 tacctttatc tctgtgcaat aacaatcatg cagagaaata ccatccgaaa aatttagggg
     7801 ctgtactaga ttagcagata tgttaccctc gaaatatgaa gataacgcac tgcaaatgaa
     7861 agaaaaaagt acagaaagaa ctgctccgtt tttttgtact cgaagttacc gcccgttctg
     7921 ccgccgatat tttgggtatc catcccaatt cggcagcact gttctaccgt aaaatccgca
//
LOCUS       DAWFJW010000089         7866 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFJW010000089 DAWFJW010000000
VERSION     DAWFJW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.089"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..108
                     /locus_tag="WGR19_001584"
     CDS             <1..108
                     /locus_tag="WGR19_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEZ0799084.1"
                     /translation="VPKKAETISNKLFFTHHPKRQTEFAVEQIRQARAE"
     gene            202..1497
                     /gene="hisS"
                     /locus_tag="WGR19_001585"
     CDS             202..1497
                     /gene="hisS"
                     /locus_tag="WGR19_001585"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0799085.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLFLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRTQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            1498..2127
                     /locus_tag="WGR19_001586"
     CDS             1498..2127
                     /locus_tag="WGR19_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0799086.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RATSQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISTAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            2281..3738
                     /gene="der"
                     /locus_tag="WGR19_001587"
     CDS             2281..3738
                     /gene="der"
                     /locus_tag="WGR19_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0799087.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPESEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDVADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQIKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3769..4668
                     /locus_tag="WGR19_001588"
     CDS             3769..4668
                     /locus_tag="WGR19_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0799088.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            4720..5136
                     /locus_tag="WGR19_001589"
     CDS             4720..5136
                     /locus_tag="WGR19_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799089.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGGVGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVSANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIQEVKLPKNK"
     gene            5202..5768
                     /locus_tag="WGR19_001590"
     CDS             5202..5768
                     /locus_tag="WGR19_001590"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0799090.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICKTSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5832..6536)
                     /locus_tag="WGR19_001591"
     CDS             complement(5832..6536)
                     /locus_tag="WGR19_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0799091.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKLKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            6682..7866
                     /locus_tag="WGR19_001592"
                     /pseudo
     CDS             6682..7866
                     /locus_tag="WGR19_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271049.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2091 a         2155 c         1887 g         1733 t
ORIGIN      
        1 gtgccgaaaa aagccgagac aatttcaaat aaattgtttt tcacacatca tcccaagcgg
       61 cagacggagt ttgcagtcga acaaatcagg caagcacgtg cagagtaagt caaatgccgt
      121 ccgcaaacca cccccaacct cacaaaccct taaaaatccg ctataatcgc ccgcaatttg
      181 atttaagcat tcttacttga aatggcacaa aaaatccaat ccgtcaaagg catgaacgac
      241 cttctgcctg tcgagcaaaa agatttcaaa ctgacggctg cgttttggca ggcgtttgaa
      301 gatacggtcg gccgctggac acgcacttac ggttatcagc aaatccgtac gccgattgtc
      361 gagcaaaccg gtttgtttgt ccgctccatc ggcgaggaaa ccgatgtggt cggcaaggaa
      421 atgtacacct tctccgattc aaacgattct ttgagtttga gcttgcgtcc tgaaggtacg
      481 gcttcctgcc tgcgtgcggt ggtcgaacac aaccttctct acaacagccc gcaaaagctg
      541 tggtatatgg ggccgatgtt ccgccgcgag cgtccgcaaa aaggccgtta tcgtcagttc
      601 catcaggtcg gtatcgaagc tttgggtttt gaaggtccgg atattgatgc ggaaatcatc
      661 gcgatgtctg ccgacttgtg ggaaaaattg ggtattcgcg aatacctgac tttggaaatc
      721 aacagcttgg gcaaccgtga ggaacgcgcg gcacaccgtg cggcattggt tgaatatctg
      781 acccgttatg aagataaatt ggatgaagac agcaaacgcc gtctgaaaac caatcctttg
      841 cgcgttttag atacgaaaaa ccctgatttg caggaaatct gcaatgccgc gccgcgtttg
      901 gtggattact tgggcgaggc ttcgcaaaac cactatgcac gcttcaaggc gatgttggat
      961 ggtttgggta ttcaatatat tgaaaatccg cgcttggttc gcggtttgga ttattacaat
     1021 cagacggttt ttgagtggac gaccgacaaa ctcggcgcgc aggcgactgt gtgtggcggc
     1081 ggccgttacg atggtttgat tgaagaactt ggcggcaaac ctgcgccgtc tatcggcttt
     1141 gcaatgggca tcgaacggtt gctgtttttg gtgagcgaat acggctcgct ggaagtgaat
     1201 gctgcgcctg atgtctatgc aatgcaccaa ggcgaaaggg cggacttgca agtgatgaaa
     1261 tacgcgcaag ccttgcgcac acaaggtttc aatgtaatgc agcattccgg ctatcaaagc
     1321 ctgaaagcgc aaatgaaaaa agccgacaac agcggcgcgc gctttgccct gattgtcgcg
     1381 caagacgaac tggcgaacgg tacggttacg ctcaaagata tgaacggcgc acacggtcag
     1441 caaactgtcg ccgccgctga tttgacaaac actttacaac aatggaagaa cgcataaatg
     1501 gcagcccatt tggaagaaca acaagagttg gacaacttta aatatttttg gaaaaccacg
     1561 ggcaaatggc tgtttgccct gctgattttg gcggcgctcg gctacttggg atacacggtt
     1621 taccaaaacc gtgcgacttc ccaaaatcag gaagcggcgg cggtgctggc aaacatcgtg
     1681 gaaaaggcgc aaaacaaagc cccgcaaagc gaaatcaatg ccgaattgac caaactccag
     1741 caaagctacc cgcattccat ttccaccgcc caagccacgc tgatggcggc ggcaaccgaa
     1801 tttgacgcac agcgttacga tgttgccgaa ggccatttga aatgggtgtt gtccaaccaa
     1861 aaagacagcc tgattcaagc gttggcggcg cagcgtctgg gcgttgtgtt gttgcaacaa
     1921 aaaaaatacg atgccgcgct tgccgcgctc gatacgccgg ttgaagcgga cttcgccccc
     1981 ctgctgatgg aaaccaaagg cgatgtctat gccgcacagg gaaaaagcca ggaagcctta
     2041 aaaaactacg gacaggcttt agaaaaaatg cctcaagatt ctgtcggtcg cgaattggtt
     2101 caaatgaaac ttgattcgct gaaataaatg ccgtctgaaa tgctgtccat tatttatcag
     2161 acggcatttt accctccctc tttatggcct cttgccgtga agaacctatt taccgtgtcc
     2221 aacggacacc tgctcgtgtt cagttctccc gaacggcact tgacacagaa agactcaatc
     2281 atgaaaccaa ccatcgcgct tgtcggccgc cccaacgtcg gcaaatctac tttattcaac
     2341 cgtttgacgc gcaccaaaga cgcgctcgtg catgacctgc ccggcctgac gcgtgaccgc
     2401 cattacggac acggcaaagt cggcagcaaa ccttatttgg tcatcgatac cggcggtttt
     2461 gagccggttg tggacagcgg cattttgcac gaaatggcaa aacaaacctt gcaggctgtc
     2521 gatgaagccg atgcggttgt gtttttggtg gacggccgta ccggtttaac accgcaagac
     2581 aagattattg ccgaccgttt gcgccaaagt ccgcgccctg tttatttggc ggtgaataaa
     2641 ggcgagggcg gcaatagggc cgtgcttgca gcagagttct acgaacttgc tttgggcgac
     2701 ccttatgtta tttcaggtgc gcacggcgat ggcgtgtatt atctgattga agatattttg
     2761 gaaaccttcc cagagtcgga agccgaagaa gccgatgcaa aacatcctgt ttttgccgtt
     2821 atcggtcgtc cgaatgtcgg caaatcgacc ttggtaaacg ccattctcgg cgaagagcgt
     2881 gtgattgcct tcgatatggc aggcacgacg cgcgacagta tccatattga ttttgaacgt
     2941 gaaggcaaac cctttaccat catcgatacc gcaggtgtgc gccgtcgcgg caaagtggat
     3001 gaagcagtgg aaaaattctc cgttatcaaa gccatgcagg cggttgaagc cgcaaacgtt
     3061 gccgtcttgg tattggacgc gcaacaagat gtcgccgacc aagatgcgac gattgcaggt
     3121 ttcgctttgg aagcaggccg tgccttggtg gttgccgtca acaaatggga cggcatcagc
     3181 gaagagcggc gcgagcaaat caaacgcgac atttcccgca aactgtattt cctcgatttt
     3241 gctaagttcc actttatttc cgcgttgaaa gaacgcggca tagacggatt gttcgacagc
     3301 attcaagctg cctacaatgc ggcaatgatt aagatgccga cgccgaaaat cacgcgcgtc
     3361 ctgcaaagcg cgatcgagcg tcagcaaccg ccgcgtgctg gcttggtgcg cccgaaaatg
     3421 cgctatgccc accaaggcgg tatgaaccca cccgtaattg tggtacacgg caattcgcta
     3481 cacgcgattt ccgacagcta tacgcgctat ctgacccaaa cattccgcaa agccttcaac
     3541 ctgcaaggca cgccgttgcg aattcaatac aatgtttcgg aaaacccgta tgaaaatgcg
     3601 gaagacaaac cgaagaaaaa accgctgcgc cgcgtcagcc tgagcaaccg tattgagaaa
     3661 cgcgaaggcc gcaaggaaga gaaaaaccgt tttaaaaaga aaaccaaagt cagtgtgaaa
     3721 aaacaattca gcaaataatt cccgacattt caaacaacag gaaacattat gtggttcaag
     3781 cagattagtt tttatccgct caacaaagaa aagctgcctg aggcggacgt acttgccgac
     3841 aaacttgctg aagctgaatt tacccattgc caaggcttag actggttcag cgaaggcttt
     3901 accgcaccgg tttcattctc cccggaactc gttttccctg ccgactttac cttgcgcgtc
     3961 gccctgaaaa aagaggaaaa agtcctgcct gccggcgtca tccgcgatat tttggaagag
     4021 aaggtagcgg aaatccaaaa caatgaagcc cgcaatgtcg gtcgcaaaga aaaacaagag
     4081 cttaaagaac agattaccga cgacctgctg ccccgagcgt ttacccgcag cagccgtaca
     4141 gaagcggtgt ttaacactcg ccacggctac ctgctcgtca ataacgcggc ttccgccaaa
     4201 gcagaaaaca tcctgaccaa gctgcgcgaa gctttgggcg gtttggaagc ctcactgccg
     4261 aataccaaac aatcgccctc ttccctgatg accggctggc tgttgcaagg gcattgcgaa
     4321 ggcggttttg aattggacag cgattgcgaa ctcaaaggca cgggcgatat tgttcccgtc
     4381 gtcaaagtat ccaaacaaga tttaaccgcc gacgaagtgg ttcaacacgt caaaaacggt
     4441 aaaaccgtaa cccagctcgg tttggtttgg cgcgaacaaa tcgcctttat cctcacgcaa
     4501 gacttcacac tcaagcgcat ccaatacctc gacgtattgc aggaagaagc cgaaagcaac
     4561 ggcgacgatg ccgccggtct cgccttcgcc tcgcaaatcc tgatggcgga atccgtcagc
     4621 atcatgttgg aagagctggt ttcctatttg ggcggctggc aagattgatt tttcagacgg
     4681 cttttatacc gtcagccaaa atccaatgga ataaccctta tgcttaaaca tctcgcattc
     4741 ctactgcccg ccatgatgtt cgccctcccc acttcggccg ccgttctgac ttcctatcaa
     4801 gaacccggct gcacctacga cggcggagtc ggaaaagacg ggcttccttc aggcaaaggc
     4861 atatggcgtt gccgggacgg gcgcggttat accggttcgt ttaaaaatgg caaattcgac
     4921 ggacaaggcg tttataccgt ttccgccaac cgcgaaatat ttatcgaacc gttcaattcc
     4981 gacagtacca aattccgcaa catggtactc tcgggcacgt tcaaaaaagg cctggcacac
     5041 ggcaggttta ccgtctcgca aaacggcgaa accctcttca ttatgaaatg tgaaaacggc
     5101 atgattcaag aagtgaaact gcccaaaaac aaataagccc cgttttcaga cggcattccc
     5161 gaatgctttg tccgaacacc gcaagaaaca aggaaaacat tatgagcatc aagtccgaca
     5221 aatggatacg ccgaatgagc gaagaattcg gcatgatcga ccctttcgag ccgaaccaaa
     5281 tcaaagaagc cgacggcaaa cgcatcatct cctacggtac gtccagctac ggctacgaca
     5341 tccgctgcgc aaacgaattt aaaatcttca ccaacatcaa cagcaccatc gtcgatccca
     5401 aaaacttcga cccgaaaaac ttcgttaccg ttgaagacga ctgctgcatc atcccgccca
     5461 attccttcgc actggcgcgc acggtcgaat atttccgcat cccgcgcaac gtcctgaccg
     5521 tctgcttggg caaatccacc tacgcccgct gcggcatcat cgtcaacgtt accccgttcg
     5581 agccggaatg ggaaggctac gttaccctcg agttttccaa caccaccccc ctgcccgcca
     5641 aaatctacgc cggcgaaggc gtggcgcaag tcctcttctt cgagagcgac gaaatctgca
     5701 aaacctcata caaagaccgc aacggcaaat atatggggca aaccggcgtt accctgccta
     5761 aagcctgaaa tataaatgcc gtctgaaccg tatcatccgg tttcagacgg catctacctg
     5821 tttatccgtg gttagaactg aaccgtccca ttgacgctga tgctgatttc ctccacaccc
     5881 tgcgcggcag aatccgcacc ctccatattg acgcttgccg ccatcggcat ggcacgaagc
     5941 atttttgcct gagcagctcc ccctcccgcg atatggctgc cgatgtgtcc caaatttaat
     6001 ttgacgattt tataaccgga cgcacccaaa atgcccgcca gtttttcggc acgcgtctta
     6061 aaacgcaaaa tagcatcctt gctgacctga tcgatgacct cgtttcggcg ttcacgcgac
     6121 acgctgaaat cggtatattc aagcgaagca tccgtctgaa catcagcaat aaaacggttc
     6181 aacgcatcaa aatccctacc ttcgacctta aattccgcac gctcctccca gcctgtttga
     6241 atgcgtctgc cgttggtata ttgatagcgc ggcatcgcac tgcgcgatac caattcggtt
     6301 ttaaagctgc cgtttttcaa ttttctgtta aaactgttga attttttaac aaactcggca
     6361 ttgacggcat ttttgtcccg tccttccgcc gccacttgga aacgtgcgga cattgtatcc
     6421 tgagccacct cgacacccgc cgattcggaa aattcgacaa tattgtaatt caatgcttca
     6481 gccgctgccg gaaaagatac cgccaatagg gaagccgcca aaatagaacg caacatatcc
     6541 gtatcctcac tcataaaacc gccaatctag catatcggcg cgctaaattg cattagcgta
     6601 aggcaacaat ttaaatattc ggtctgcgtc tgtcttataa taaccgcctt tccgatacgg
     6661 aatcccacta tgcccaaacc cttgcacgtc ctcgtcatcc cctcatggta tccgcaatcc
     6721 gaacaggatg tggacgggat ttttttcaaa atcaggcact ggcattgcag aggaaaggca
     6781 tcaaaaccgc cgtgcttgca ccgatgttcc gctacttgcg gaaagaaaca gcaagcatcc
     6841 tgaccgacct tacggttttg caaaatatcg gcaaagcggt ttggacatct atgcatggca
     6901 cggcatgtat ttcttccccc gctttccgtt catcgacatc gaccgcatcc gctgggtgcg
     6961 cgtgggtttg aaagccttca aacactacat ccgcgaaaac gggctgcccg acctgattca
     7021 cgcccaccgc atgaactatg ccggcatact cgcccaaaaa atttcccaaa aatacggcat
     7081 cccctatgtc gtcacgaaac acagcagcac cattacgcgc ggtctgatcc gtccccacca
     7141 atggcagcct atgaaaaatg cggcggcgca cgccgccgca cgtctcgcca tcagccgcca
     7201 tttcgcacac gtcctgcaac acaaatacgg ctgcgaatgg cagtacctcc ccaacatact
     7261 gggcggaata ttcaaccgac cgtttgaaca aagagaaaaa aacaacaaac cgcatttcgt
     7321 gttctgcacc gtctcgcacc ttcgccgtct caaaggacac gatgtcctgc tcactgcctt
     7381 tgcccgggcg ttggcacaat gcccgcaact gcgtctaaac atcggcggca gcggacagga
     7441 agaacagcgg ctgaaacagc aggcggcaga cttgggcatt acccatgccg ttacattttt
     7501 aggcgcactg caacccgaag cagtcttgga tttgatgaag aacagcgacg catttgtcct
     7561 tgccagccgc accgaaacct tcggcgtggt ctatatcgaa gcactgtccc aaggattgcc
     7621 cgtcattgcg acacgctgcg gcggtgcgga atctattgtt tcagacggca acggatattt
     7681 ggttcctgtt gacgacgacg atgcccttgc cgacgcactc atcaaaatgt atgaacacca
     7741 ctctgatttt gaacctgccc gacttaggga aaactgtctg aacgaatttg gcgaaaatgc
     7801 cgttataggc aggttgatcg gcattttccg acaggcaatc gcagaatacg gtaagaaaat
     7861 accggt
//
LOCUS       DAWFJW010000090         7858 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFJW010000090 DAWFJW010000000
VERSION     DAWFJW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.090"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            27..1142
                     /gene="asd"
                     /locus_tag="WGR19_001593"
     CDS             27..1142
                     /gene="asd"
                     /locus_tag="WGR19_001593"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0799092.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            complement(1371..1901)
                     /locus_tag="WGR19_001594"
     CDS             complement(1371..1901)
                     /locus_tag="WGR19_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0799093.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(1912..2256)
                     /locus_tag="WGR19_001595"
     CDS             complement(1912..2256)
                     /locus_tag="WGR19_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799094.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            complement(2243..3022)
                     /gene="xth"
                     /locus_tag="WGR19_001596"
     CDS             complement(2243..3022)
                     /gene="xth"
                     /locus_tag="WGR19_001596"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0799095.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            complement(3105..4526)
                     /locus_tag="WGR19_001597"
     CDS             complement(3105..4526)
                     /locus_tag="WGR19_001597"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0799096.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFSAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            complement(5214..6263)
                     /locus_tag="WGR19_001598"
     CDS             complement(5214..6263)
                     /locus_tag="WGR19_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0799097.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            complement(6346..7500)
                     /gene="obgE"
                     /locus_tag="WGR19_001599"
     CDS             complement(6346..7500)
                     /gene="obgE"
                     /locus_tag="WGR19_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0799098.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
BASE COUNT         1822 a         2007 c         1952 g         2077 t
ORIGIN      
        1 acgatttttc ctcaaggagc aacacaatga aagtaggttt cgtcggctgg cgcggtatgg
       61 tcggttcggt tttgatgcag cgtatgaaag aagaaaacga cttcgcccac attcctgaag
      121 cgtttttctt taccacttcc aacgtcggcg gcgcagcccc tgatttcggt caggcggcta
      181 aaacattatt agatgccaac aatgttgccg aactcgccaa aatggacatc atcgttacct
      241 gccaaggcgg cgattacacc aaatccgtct tccaagccct gcgcgacagc ggctggaacg
      301 gctactgggt tgacgcggca tcttctctgc gcatgaaaga cgacgcgatt atcgtcctcg
      361 accctgtcaa ccgcaacgtt atcgacaacg gcctcaaaaa cggcgtgaaa aactacatcg
      421 gcggcaactg caccgtctcc ctgatgctga tggctttggg cggcctgttc caaaacgatt
      481 tggtcgaatg ggcaaccagc atgacctacc aagccgcttc cggcgcaggt gcgaaaaaca
      541 tgcgcgaact catcagcggt atgggcgcgg ttcacgccca agtggcggac gaacttgccg
      601 atcctgccgg ctcgattctc gacatcgacc gcaaagtatc cgatttcctg cgcagcgaag
      661 actatccgaa agccaacttc ggcgtaccgc tcgccggcag cctgattccg tggattgacg
      721 tggatttggg caacggccag tccaaagaag aatggaaagg cggcgtggaa accaacaaaa
      781 tcctcggccg cagcgacaat ccaaccgtga ttgacggcct gtgcgtccgc gtcggcgcga
      841 tgcgctgcca cagccaagcc atcactctga agttgaaaaa agacctgcct gtttccgaaa
      901 tcgaaacgat tttggcaggc gcgaatgact gggtgaaagt catccccaat gaaaaagaag
      961 ccagcatcca cgagctgact cctgccaaag ttaccggcac gctgtccgtc cctgtcggac
     1021 gcatccgcaa actgggcatg ggcggcgaat acatcagcgc gttcaccgtc ggcgaccagc
     1081 tcttgtgggg cgcggccgag ccgctgcgcc gcgtattgcg tatcgtgttg ggcagcctgt
     1141 aagccctgtt tgaatggaaa tgccgtctga agcctgtttc agacggcatt ttccttgcaa
     1201 ccctgccgga taacgccctg ccggcactgc cgacgtaaaa aataaaggat tccatttccg
     1261 gcggtatgcg gcagcccgac tttatccgaa cctgatgcgc ctgcacgtca atgaaaacag
     1321 cccgatatgc ggacttcctg ctacagccga aattccgata aggcaagcgt tcacgccagc
     1381 aacatttcct gcatcagctt cataccccac tgccagccgc cgagcatacc gttcaaactg
     1441 cccgaatgcg gggaaaccaa caggcgcgcg ttccacgtat ccgcctgttt ttgcgcccaa
     1501 tctcgcggca catccccgtg ttcggacacc accaatgcgg cacggcaggg acagcggacg
     1561 cgttggaaag tgtgttccgc atcgtcggga aaaatatcgg gacgctgcgg tacaaggatg
     1621 atgttggcaa ttttcttccg tgtcaggatg tctgcctgat acagccacgc caaaaatgcg
     1681 gccgcgcccg caccgtgtgc gacaacggcg acgtatttgc cacgtatgcg ttcaaatgcc
     1741 gtccgaagcc ctgcctgcca ttcccctatg ctttgaccgg ccgacgcttc ggacatctgc
     1801 acgacgggat aactgatcgc ccaacggtct atccacatct gatcctctcc ggcatcgcgt
     1861 atcagccaaa gcgtcaaatc ttcgagttca aaaccctgca taccgccccg cctatttcag
     1921 caggtcccgg agggtaaagg cgatgagcag cgaagcgggt acgctcaata tggcgcagac
     1981 ggtcaggcag gcaaaaatat tcaccacccg ccgccagcct ttccaaccca aacatttgga
     2041 cgcaatcagc aggcagggca tgcagatcag cagccacacc aacgcccatg tcgggtttgc
     2101 cttggtcggc gcaagccagc cttgcatccg cgacaacata aatatcgccc acaccaatat
     2161 gggcaggata aacgcagcga caacccatgc tgcgcctatt cctgtttttc cgtccacatt
     2221 ccaatcatat ttacccaaaa ctttattcgg cagcatagtc atactccatg accagcggcg
     2281 catggtcgga aaatttttca tctttataaa cgtgtgcgga cacggctttg gcggcaagtt
     2341 cgggcgtaac catctgataa tcgatgcgcc acccgacatc tttcgcatac gcctgcccgc
     2401 ggttgctcca ccacgtgtag cccggcacat cgggataaag cgtgcgccac atatccgtcc
     2461 aaccgagctt gtggataacc ttgcctatcc actcgcgttc ttccggcagg aaacctgaat
     2521 ttttctgatt gcctttccag tttttcaggt cgatgttttg gtgggcgatg ttccagtcgc
     2581 cgcaaacgac aatgtcgcgt ccttcgtttt tcatcgcttc gagcataggg taaaacgcat
     2641 caaggaaacg gtatttcacc tgctggcgtt cttccgcgct gctgccgctg ggcaagtaaa
     2701 gcgaaataac gctcaacctg tcgaaatcgc aacgcacaaa tcgcccttcc ctgtcgaatt
     2761 cttcaatgcc cataccgatt tgcacattgt cgggtttgcg tttgctgtac accgccacgc
     2821 cgctgtaacc gcgtttctcg gcgcaatgcc aatggccgtg catcccatgc ggatttttca
     2881 tatcggcaga caaatcagcc tcctgcgctt tgagttcctg cacgcagaca atgtccgcgc
     2941 ccgatgcggc gatgtattcg taaaaacctt ttttgtaggc ggagcggatg ccgttgacgt
     3001 tggcggaaat gattttaagc ataataaaaa taagttctca caataaaaat gccgtctgaa
     3061 caaaaaaggg caaaatgcgg cacatttacc cttttcgatg gattttaacc gcgccgccaa
     3121 gtcgtgccgc cggcgttgtc ttccaaaatg attttgtgtt cgttcagaag gtcgcggatg
     3181 cggtcggatt ccgcccagtt tttatcggcg cgcgcctgtt tccgccgggc gatcaagtct
     3241 tcgatttctt cgttggagag gccgtctgaa gctgcgctgc cttgcaggaa ctcggtcggg
     3301 tcgcgttgca gcagtccgat gatgccgccc aaggctttca ggcagccggc gagctgtgcg
     3361 tcattggttt tgttcacttc gcctgccagc tcaaacaaca cggcaaccgc ttcaaccgta
     3421 ccgaaatcgt cgttcatggc ggcgtagaag cggcgggtgt agtcgttggc gttttcagac
     3481 aaatcaaacg cggcggcagg cgtgtttttc aaggtggtgt atagacgggt caacgcgcct
     3541 ttcgcgtcgt ccaaatgcgc gtcggaatag ttcaacgggc tgcggtagtg ggcgcgcagg
     3601 atgaagaaac gcacaacttc agggtcgtat tgtttcaata cttcgcggat ggtgaagaag
     3661 ttgcccagcg atttcgacat tttttcgcca tccacgcgga tgaagccgtt gtgcagccag
     3721 tatttgacgt ggctggcgat gctttgaccg tgatgggttt gcgcgtggtc gtggccgcag
     3781 gtatgaccgc tggcaccgac gctttgggcg atttcatttt cgtggtgcgg aaactgcaaa
     3841 tccgcaccgc cgccgtggat gtcgaaagta tcgccgaaca ggttttcact catggcggag
     3901 cattcaatgt gccaacccgg acggccattg ccccacgggc tttcccatgc cggctcgcct
     3961 gctttggccg ctttccacaa cacaaaatca agcggatcgc gtttgaaacc gtccacctcc
     4021 acgcgctcgc ctgcgcgcag gtcgtccaac gatttgcccg acaattggcc gtaagcggaa
     4081 aactcgcgca cggcgtagta aacatcgccg tttgcggcag gatatgcctt gccgttttga
     4141 atcagggttt caatcatggc aatcatttgc ggaatgtttt ccgtcgcctt cggctcaata
     4201 tccggacgca acacacctaa agcatcggca tcttcgtgca tagcctgaat gaaacgcgcg
     4261 gtcagttcgc cgatggtttc gccgttttca gccgcacggg cgatgatttt gtcgtcgatg
     4321 tcggtgatgt tgcgcacata agtgagcgga taaccgcact cacgcaacca acgggcaatc
     4381 atgtcgaaca ccaccatcac acgggcatgg cctaagtggc aatagtcgta aacagtcatg
     4441 ccgcaaacgt acatacgcac gttttcaggg tcgatggggg caaagggttc tttttggcgg
     4501 gtaagggtgt tgtagatggt ggtcatggga ttatggatta atctttgttg ctcggatgat
     4561 aatttctgtt ctgttcctgt agatacggac caaggaacat tacgtagttg cggattatta
     4621 atatggttga tatttgtgaa aattggttct gcataacagt ttgcaaaatt ttttgtaaat
     4681 tctgataatt taaacttatc ttttaataag tttgctaaat ctgatgacga gggataaagt
     4741 ttacttctta tattaggcat ttcaatatga aggactattt ttatttcgtt acaatctaaa
     4801 gccaagcgag aaaaatcttt ttcttcctgt ttttctgctt taaatttagc agaaaccaat
     4861 cctgccaatg aatctcgaat ttttcttgcg attaaatatg gtaggtcaga aagtttttca
     4921 tctattgatt gggcatttgg ctcaagccca agtcggtaat aatctttaat ttcgattagc
     4981 caaagtgtcg actcatgaag ggctattata tctacacctg agctgccatt atcgtcatct
     5041 acactttgat ttatcccgtt ctttcccttt tcatttgtat caattttatt acgtaaatta
     5101 caactgttct gaaaaatttt ataatgttcc cattcgtcat acttggtaac gtaataatct
     5161 tcaggaaaag caaaggttaa tctcttttct gtgattgtag tcatagctta acctcaaata
     5221 ttcagatacc tgtctgcctg cataatgttt tcatctaaca atatcaatgt gttcaaatca
     5281 ttaatactgt tcccttgctc cacttttgtt ccatcatcgg aagcaatcaa cgagaaaaaa
     5341 cgtacaggta aatccgtgtt attttcaagc ttcaaaagtt ccaattctct caataagaat
     5401 agagagtgtg ttgcaataaa aacctgaata ccctgttgag ataaagacca aataatacgg
     5461 gcagccactt tgatcaattt aggattcaga ttagcttccg gttcatccca aaatagatag
     5521 cctttatcca gcaatgcccc tgttgcgatt aaccgggcaa tcatgacaaa tttccgcaaa
     5581 ccctctgcta ccaaaggtgc ttcaatctta ccgcccgtat ttgtcagcga tagataaaac
     5641 cttccttgtt cttcagatac ttttccgccc atcgcgttct caataggttc gagcaattct
     5701 cgaatttttg tttctctggg gcctttggca agcgggtgat ttaattgcat acaggtatca
     5761 aaccaagttt cttcgaaagg gatgctttgg ttttgataca aagaagtgaa ccaaggacaa
     5821 agtgtaatta attcgcggct gggtaagaag ataggtgtcg gagtatattc attttctttc
     5881 aatccgatgc tttgaacatt gacttgcgat gatgagttac tggaaaaatt cagactacta
     5941 tgcgtagtgc cgttttgcag ttttaaaacg atttccgtac gcccgcgccc ctgcaaacgt
     6001 ttgctcaacc tgcccaagga atcgggacgg aaaacattca gtaatttatc ggcaaaactt
     6061 ttttgcaatt ctgttttcag taatctgttt ttggtgttag atgttacttc tagcaggctg
     6121 tataaaattt ttaacaaatg tgttttgcca caaccgtttt cggcaacaat aacattgaga
     6181 ttttcagaaa attcaaaagt atcgtttgga agaacggtaa agtttgtcaa ctcaagcgac
     6241 tggatatatt ggttagatga catttttaat ccatttcaat cttgctttaa aattgtttca
     6301 aacaaccttt tgtagaacaa atatcgtctg aaaccctttc ttttttcact ccggcttaaa
     6361 cacgcccgta tccgttttag gctgctgttc gataatttca acatttgccg ctgctttctc
     6421 cgcttcggct ttttcagctt cgatacgttt tttctcggtc aggtattggt tgatttggtg
     6481 taccaattcc tgcgtgcctt ggtgggtcag cgcgctgatt tggaagaggc gcggggtttc
     6541 catgtcaaat tggaaacggt cgtcgggttt ggggtagtcc cagccgacgg cttcgaggaa
     6601 ggcggcagtg cgcgtttggg cttcttcttc gtcaagcatg tcgagtttat tcagcaccag
     6661 ccagcgcggt ttgccgtaaa gttcttcatc gtatttgcgc aattcgttga tgatagcaag
     6721 tgcttcttcg gcagggttga cggtttcgtc gaagggcgcc aaatcgacga cgtgcagcaa
     6781 taatccggtg cgcgataagt gtttgaggaa acgatgcccc aaacccgcgc cttctgccgc
     6841 gccttcaatc aggccgggga tgtcggccat cacgaagctg tggttttcgt cgatgcgtac
     6901 cacgcccaag ttcggatgca gggtggtgaa ggggtagttg gcgattttgg ggcgtgcggc
     6961 ggatacggcg gtaatcaggg tggatttgcc ggcgttgggc atgcctaata agccgacatc
     7021 ggcgaggact ttgagttcga gttgcaggga gcgggtttcg ccttcttcgc cgggcgtgga
     7081 ttgttttggg gcgcggttga cggaggattt gaagtggatg ttgcccaagc cgcctttgcc
     7141 gcctttggct aggcagacgc gctgtccgtg gtaagtgagg tcggcaacga tttcgtcggt
     7201 gtcgaggtcg cggataaggg tgccgacggg cattttgagg acgatgtcgt ccgcacctgc
     7261 gccgtaacgg tcggaaccgt ggcctttttc gccgtttttg gcttggtagc gtttaacgaa
     7321 gcggtattcg acgagggtgt tggtgttttc gtcggcttcc gcccagacgc taccgccttt
     7381 gccgccgtcg ccgccgtccg ggccgccgcg cggtacgaat ttttcgcggc ggaaactggt
     7441 tgcgccatta ccgcctttgc ctgcggcgac ttcgattttt gcttcgtcga tgaatttcat
     7501 tcaatgctct tgtttgttgg tttcaaatgg ggggttcaga cggattaccg tgtgttttga
     7561 tgccgtccga acagaatttc ggacgctatt ataagggata agcggtattt caacacgccg
     7621 tacccaaact atttgttccg cccatcttaa tgaattttta agcaaatctt cagcctgcaa
     7681 acaaaattat gtccaacttc tttggtacaa tcgcgccttt ttgacattcc gacccgacgg
     7741 aatgtccgtt caaaccgtta catataataa gttttggggc tgtcctagat aactaggata
     7801 aactcggttt tactaattgt tttaaaatgg aacaagaact tttatctcac tgttgtta
//
LOCUS       DAWFJW010000091         7843 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFJW010000091 DAWFJW010000000
VERSION     DAWFJW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.091"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(303..1220)
                     /locus_tag="WGR19_001600"
     CDS             complement(303..1220)
                     /locus_tag="WGR19_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0799099.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFAGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITTSEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            1393..2193
                     /locus_tag="WGR19_001601"
     CDS             1393..2193
                     /locus_tag="WGR19_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0799100.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGKVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            2241..3017
                     /gene="mlaE"
                     /locus_tag="WGR19_001602"
     CDS             2241..3017
                     /gene="mlaE"
                     /locus_tag="WGR19_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0799101.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            3068..3562
                     /gene="mlaD"
                     /locus_tag="WGR19_001603"
     CDS             3068..3562
                     /gene="mlaD"
                     /locus_tag="WGR19_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0799102.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            3599..4189
                     /locus_tag="WGR19_001604"
     CDS             3599..4189
                     /locus_tag="WGR19_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0799103.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            4246..4524
                     /locus_tag="WGR19_001605"
     CDS             4246..4524
                     /locus_tag="WGR19_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0799104.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            4521..5360
                     /locus_tag="WGR19_001606"
     CDS             4521..5360
                     /locus_tag="WGR19_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0799105.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQPG
                     TQP"
     gene            5485..5847
                     /locus_tag="WGR19_001607"
     CDS             5485..5847
                     /locus_tag="WGR19_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0799106.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            5844..6230
                     /locus_tag="WGR19_001608"
     CDS             5844..6230
                     /locus_tag="WGR19_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0799107.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            6227..6715
                     /locus_tag="WGR19_001609"
     CDS             6227..6715
                     /locus_tag="WGR19_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0799108.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(6798..6965)
                     /locus_tag="WGR19_001610"
     CDS             complement(6798..6965)
                     /locus_tag="WGR19_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0799109.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(6932..7312)
                     /locus_tag="WGR19_001611"
     CDS             complement(6932..7312)
                     /locus_tag="WGR19_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0799110.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIVGRRVRGFLARRISPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGAG"
     gene            7322..7425
                     /locus_tag="WGR19_001612"
                     /pseudo
     CDS             7322..7425
                     /locus_tag="WGR19_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7550..7765
                     /gene="rpmE"
                     /locus_tag="WGR19_001613"
     CDS             7550..7765
                     /gene="rpmE"
                     /locus_tag="WGR19_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0799111.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         1859 a         2249 c         1950 g         1785 t
ORIGIN      
        1 gatataaaac aatgtagttc ataaccattt atttcaatca atactgcctc ttcaatggtg
       61 ttatctaact ttttgatttt acatttatat aacatttatc ttgcctttgg ataaattgtt
      121 ttgttttttg gattgttatt caaagttctc caaaagtctt ttcctgattt tgtatgtatc
      181 gtggtaatac taccattttc attggttcca acaaaaataa atttagattg acctttggaa
      241 tcagtaccca aaaactgaac atatccagac acgcggattt cgggttttaa atcaaatgcg
      301 gcttaccggt tttctttttt ggtttccgca ggcgtgctgc cgaaatattg cctgaatgcc
      361 tgagtaaagc tggaaacgtg gcggtagccg cataaatatg cggtttcgcc tatgcttttg
      421 cccccgtttt gcaacagata aagcgcgtgc tgcatttgtt tgtggtgcag ccactcgctt
      481 gtggtaatgc cgaaatggtc gcgcatacgg cgttgcagcg tcctttcgct gatgttcagc
      541 gcgtctgtca gccggttgac ttggtgtgcg ccgccggcaa acgcggcatt cagggtgcgg
      601 ctgaagtctt cagacggcat agcgtctgct tccgccgttt gccccgccgc cggcccgatg
      661 ccgtctgaaa ccgtgtccca caagtccgac agcagccgca acacgtccgc ctcgcggcgc
      721 aatgtttcgc ccaaatgccc ctttgggacg gctttcaggc aggatgccgc caagccgcgc
      781 aggttcgggg gcaaatccca tatcctgacc ggttcgcggt aaagcagggg tgcgaaacgc
      841 gcgtattccg gacgcagcag ccattgttcc atacctttaa tggtcatttt gaccgttttg
      901 ccgcctcggt aaagatagcg gctgaacagg acttcttccc cgacagcaat taggacaatc
      961 ttgccgccgt ccgcatcgat ttggaagcgg cagcggttga tgccgaagtc caaactgcct
     1021 tccagcaaga gcacgaacga cacataaggt tcggcgaggc ggctgctgca aaaatcacac
     1081 cgtgccgtta ctgtgccgcc gtggatggaa atgccgttgg acaaagtatc gaaacggtag
     1141 ctgccctcca catagctgcc gccgccgctt ttgaccagat tgacgaaccg gctgctgttc
     1201 aaatgttctg ctttttccat agttttctga taaaggttat catttgaaag ataacatttt
     1261 tcgccacagc aaacaatccg ccgccgacaa cggcattttc acaacattta ccgtccgtat
     1321 gccgtctgaa aattgcccgt cttcccgata agccttaaaa tacacgccgt caaacctacc
     1381 gtccgtccgt acatgagccc atcccccttt atcgaaatga aagacgtcgc ttttgcgtat
     1441 ggcgaccgcc cgattctgaa cgacatcaat ttcagcattc cgcaaggcaa ttttgccgcc
     1501 gtgatgggcg gttcgggcag cggcaaaacc acgctgatgc ggctgattac gggtcagatc
     1561 cgtccgcagt cgggcaaagt tctgatagaa gggcgtgatt tggcgggctt ttcggctgac
     1621 gaactctacg aacaccgccg ccgcatgggc gtattattcc aacacggcgc gctgtttacc
     1681 gatttgtcgg tattcgacaa tatcgctttt ccgatgcgcg aactgacacg cctgccggaa
     1741 gcggtgattc gagatttggt tttgttgaaa ctgaacgcgg tcggtttgcg cggcgtggaa
     1801 aacctgatgc cgtccgagtt gtccggtggg atgtcgcgcc gcgtcgcgct tgcccgcacg
     1861 attgcgctcg accctgaaat tatgttgtac gacgaaccgt ttaccggcct cgatccgatt
     1921 tccttgggcg tgattgccca cttgatcagc cgcgtcaata aggctttgcg ttcgaccagc
     1981 gttatggtaa cgcacgacat tgaaaaatct ttggaaatcg tcgatcaggt gatttttttg
     2041 gcgcacggcg aaattatgtt ctccggctcg ccgcaggaaa tgcgcgaact ggattcgcct
     2101 tgggtgcgcc agtttgtcgg cgggctggca gacggccccg tagcataccg ctatccggcg
     2161 caaacgtcgt tgcagcagga tttgctcggg taaatgagca ggtgccgtct gaaaccgttg
     2221 aactttacat atagaaattt atgaatttta tccgttccgt cggggcgaaa accctcggcc
     2281 ttattcaatc tctcggcagt atcacgctgt ttctgctgaa tattctggcg aaatccggta
     2341 cggctttcgt ccgtccgcgc ctgagcgtgc gccaagtgta ttttgccggc gtgctgtcgg
     2401 tgttgattgt tgccgtttca gggctgtttg tcggcatggt cttgggtttg cagggctata
     2461 cgcagttgtc gaaattcaaa tccgccgata ttttgggcta tatggtcgcg gcttcgctgt
     2521 tgcgcgaact gggtccggtg ttggcggcga ttctgtttgc cagcagcgcg ggcggtgcga
     2581 tgaccagcga aatcggtttg atgaaaacga ccgaacagct cgaagcgatg aacgtgatgg
     2641 cggtaaaccc cgtcgcccga gtggttgcgc cgcgcttttg ggcgggcgtg ttttccatgc
     2701 cgcttttggc ttcgattttc aacgtggcgg gtattttcgg cgcgtatttg gtcggtgtaa
     2761 cctggctggg cttggacagc ggtattttct ggtcgcaaat gcagaacaac atcacgatac
     2821 attacgatgt aatcaacggt ctgatcaaat ccgccgcgtt cggcgtggcg gtaacgctga
     2881 ttgccgtgca tcagggcttc cactgcgtcc cgacctcgga aggcattttg cgcgccagca
     2941 cgcgcacggt ggtttcgtcc gccctgacga ttttggcggt cgattttata ttgaccgcgt
     3001 ggatgtttac agattgacag gccgtctgaa gacgaaacgc ggaacatcgg atattcaagg
     3061 aactttaatg aaaaagaaca tattggaatt ttgggtcgga ctgttcgtcc tgattggcgc
     3121 ggcggcggtt gcctttctcg ctttccgcgt ggccggcggt gcggcgttcg gcggttcgga
     3181 caaaacttac gccgtttatg ccgatttcgg cgacatcggc ggtttgaagg tcaatgcccc
     3241 cgtcaaatcc gcaggcgtat tggtcgggcg cgtcggcgct atcggacttg acccgaaatc
     3301 ctatcaggcg agggtgcgcc tcgatttgga cggcaagtat cagttcagca gcgacgtttc
     3361 cgcgcaaatc ctgacttcgg gacttttggg cgagcagtac atcgggctgc agcagggcgg
     3421 cgacacggaa aaccttgctg ccggcgacac catctccgta accagttctg caatggttct
     3481 ggaaaacctt atcggcaaat tcatgacgag ttttgccgag aaaaatgccg acggcggcaa
     3541 tgcggaaaaa gccgccgaat aaacgtatgc catcatattc aaactaggac acttaatcat
     3601 gaaaaaatcc tccttcatca gcgcattggg catcggtatt ttgagcatcg gcatggcatt
     3661 tgccgcccct gccgacgcgg taaaccaaat ccgtcaaaac gccactcaag tattgagcat
     3721 cttaaaaagc ggtgatgcca acaccgcccg ccaaaaagcc gaagcctatg cgattcccta
     3781 tttcgatttc caacgtatga ccgcattggc ggtcggcaac ccttggcgca ccgcgtccga
     3841 cgcgcaaaaa caagcgttgg ccaaagaatt tcaaaccctg ctgatccgca cctattccgg
     3901 cacgatgctg aaattaaaaa acgccaacgt caacgtcaaa gacaatccca tcgtcaataa
     3961 aggcggcaaa gaaatcatcg tccgcgccga agtcggcgta cccgggcaaa aacccgtcaa
     4021 catggacttc accacctacc aaagcggcgg taaataccgt acctacaacg tcgccatcga
     4081 aggcgcgagc ctggttaccg tgtaccgcaa ccaattcggc gaaattatca aagcgaaagg
     4141 cgtggacgga ctgattgccg agttgaaggc taaaaacggc agcaagtaat ccataagcca
     4201 tgccgccaga ctgaagatgg catggatttt ttcctaccga acactatgca tacagaactc
     4261 aacaacggaa cactgcacat cagcggcgac gtaaccgtca aaaccctgac cgccgccgcc
     4321 tttacccgct tcagacagca atgtcgtctg aaagacaccc gcgcggttga tttgagcggc
     4381 gtcggacgcg ccgattccgc ctgcgtgtcg ctgctgctcg aagtgctgcg cggttgcaaa
     4441 ggcagcgtca ggctgaccgg cattcccgaa tccgtgcgcg cgctgtccga actgtacgaa
     4501 atcaaagact ggctgaaatc atgaaaaaaa ccgcctatgc cttcctcctg ctgatcgggt
     4561 tcgcttccgc ccctgcattt gccgaaaccc gccccgccga cccttatgaa ggctacaacc
     4621 gcgccgtttt caaattcaac gaccaagccg accgctacat tttcgcccct gccgcgcgcg
     4681 gctaccgcaa agttgcgccg aaacccgtcc gcgccggcgt gtccaatttt tttaacaacc
     4741 tgtgcgacgt ggtcagcttc ggcagcaata tcttgcgctt agacatcaaa cgcgcaagcg
     4801 aagaccttgt ccgcgtcggt atcaacacca ctttcggttt gggcgggctt atcgacatcg
     4861 ccggcgcggg cggcattccc gacaataaaa acaccttggg cgacacgttt gcttcgtggg
     4921 gatggaaaaa cagcaattat ttcgtgttgc ccgtcttagg gccgtccacc gtccgcgacg
     4981 cgctcggcac gggtattacc tccgtttatt cgcccaagaa tatcgtcttc cgcacccctg
     5041 tcggacgctg gggcacgact gccgtatccg ccgtcagtac gcgcgaaggc ctgctcgatt
     5101 tgaccgacag tctggacgaa gccgccatcg acaaatacag ctacacgcgc gacctctata
     5161 tgaaagtccg tgcgcggcag accggtgcaa cacctgccga aggtacggaa gataacatcg
     5221 acatcgacga attggtcgaa agtgccgaaa ccggcgcggc ggaaactgcc gttcaagaag
     5281 attccgtatc cgaaacacag gcagaagcag caggggaagc cgaaacgcaa cctggaacac
     5341 aacctggaac acaaccttaa agccatgccg tctgaaaatc cgtgttcgga cggcattccc
     5401 ttctgtcgga acggacagtc cgaaaccaca gattgggcta taatctcttt tttgcgcata
     5461 ccgcgcgaca gcaaaggaaa aaccatgtac gaagtcaacc gcagcgtatt cgtcctcatc
     5521 cctttagaac ctttttggaa ctggctgcaa accctgccgg gcaaccacct cgacggactg
     5581 actcttgaag acatccaagc cgacgccaat tcctaccttg tccgcccgtg cgaaaccgcc
     5641 gacgaagtat gggacgaaat cgaagcccgt tttgaagaca ttttcgccgc cgaacttgcc
     5701 gactggtgcg aagacgaacg cgagtggccc gcgctcgatg ccgacatttt caacgaatgg
     5761 ttcgacatcc agctttctac cgtcatcacc gacctcgaac acgaaccgct tgcccgcgaa
     5821 gccttccaac ccatcaacct gaactgatga agctgaccgt ccgcaactac catctcgacg
     5881 gctacggcca tgtcaacaat gcgcgctacc tcgaattttt cgaagaagcg cgctgggcgt
     5941 tttttgaaga acgcggactg ctgcacgagt tggcaggcct catactgatt gtcgcccgca
     6001 tcgacatccg atacagccgc cccgccgtcg aaggcgacgt attgcagttt tcatgccgcc
     6061 tgaaaacccc cggcacgcgc cgtattgtgc tgacccagac gattacactg ccaaacggca
     6121 aaaccgccgc agaagccgac attaccctga tgcccgtcca cgccgccaca caacgcaccg
     6181 tcagcctgcc cgccaccctt gcccgcgcat tggaagcctt gtccgaatga aaaaaatact
     6241 caccgccgcc gtcgtcgcac tgatcggcat cctccttgcc atcgtcctca tccccgacag
     6301 caaaaccgcg cccgctttct ccctgtccga cctgcacgga aaaaccgttt ccaacgccga
     6361 cctgcaaggc aaagttaccc tgattaattt ttggtttccc tcctgtccgg gttgtgtgag
     6421 cgaaatgccc aaaatcatta aaacggcaaa tgactataaa aacaaaaact tccaagtcct
     6481 tgccgtcgcc cagcccatcg atccgataga aagcgtccgc caatatgtca aagactacgg
     6541 tttgccgttt accgtcatgt atgatgcgga caaagctgtc ggacaggcgt tcggcacaca
     6601 ggtttatccg acttccgtcc ttatcggcaa aaaaggcgaa atcctcaaaa cttatgtcgg
     6661 cgaacccgat ttcggtaaac tctaccaaga aatcgatacc gcgctggcac aatagcgatg
     6721 ccgtctgaag cgcaaaaaaa acagaaacga tgttcctgtt ttttcagatc atccgccccg
     6781 acacgttcag acggcattca accttccggc agattctttt tcaccactgc cggattgcct
     6841 gccgccaaag agtagggcgg aatgtctttt gtaaccaccg cgcctgcgcc cacgaccgaa
     6901 ccgcgtccga cggttacgcc cgccattaca atcaccctgc gccccggcca gacatcgtcc
     6961 tccaacgtaa tcggacggat ttccgtgtag ccctcaaagc gttttttgaa cggtcaaact
     7021 tgtggttatt tgaataaaac agacattccg gctccatcat cacatttttg ccgaccaccg
     7081 gcccacggca gatttcacag tttgccccga tgcccgagcc gtcgcccaaa accgtatccg
     7141 ggaacacata cgccccgcgt tcgatattga ccccgcgtcc gatatgcgga gaaatccgcc
     7201 gtgccagaaa accgcgcacc cgtctgccga cgatgccgat gccgcgcata tgcgacggcg
     7261 gcaacaccga accggcaaaa cgcagcagca ggaaaaagaa ttttgatccc atcaaacctc
     7321 cctcaaaaga actttttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     7381 acagtacaga tagtacggca aggcgaggca acgctgtact ggtttttgtt aatccactat
     7441 acattttatc gaatatcctc ttgacgaaac aacagaaaat agcgagaatg ccgagcttgt
     7501 ctgaagcata ggtacatccc ctatgcccgc ttttttgaaa ggaaatcaaa tgaaacaagg
     7561 tattcacccg aactaccacg aagtcaacgt tacctgctct tgcggcaaca aattcgcaac
     7621 caaatccgcg atggaaaaag aaaacttcaa tatcgaggtt tgctccctgt gccacccgtt
     7681 ctataccggc acccaaaaaa tcgtcgatac caccggccgc gtggacaaat tcaacaacaa
     7741 attcggcaac ctgttcaaac gctgattgcc gctttgcaag aattaagccc tgccatcatt
     7801 tcgatggcag ggctttttaa tatttggcac actgacttct tat
//
LOCUS       DAWFJW010000092         7682 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFJW010000092 DAWFJW010000000
VERSION     DAWFJW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.092"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(52..807)
                     /locus_tag="WGR19_001614"
     CDS             complement(52..807)
                     /locus_tag="WGR19_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0799112.1"
                     /translation="MKYVRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(932..2077)
                     /gene="nirK"
                     /locus_tag="WGR19_001615"
     CDS             complement(932..2077)
                     /gene="nirK"
                     /locus_tag="WGR19_001615"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0799113.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAASAEAASSAAQAAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAVAEQPEYVVFNGHVGSIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAKSDY"
     gene            2451..4706
                     /locus_tag="WGR19_001616"
     CDS             2451..4706
                     /locus_tag="WGR19_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0799114.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTISAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            4885..5418
                     /locus_tag="WGR19_001617"
     CDS             4885..5418
                     /locus_tag="WGR19_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0799115.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5481..6665)
                     /locus_tag="WGR19_001618"
     CDS             complement(5481..6665)
                     /locus_tag="WGR19_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0799116.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGTRMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSEFGKLSPSVFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6705..7109)
                     /locus_tag="WGR19_001619"
     CDS             complement(6705..7109)
                     /locus_tag="WGR19_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0799117.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHE
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            complement(7286..7429)
                     /locus_tag="WGR19_001620"
     CDS             complement(7286..7429)
                     /locus_tag="WGR19_001620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799118.1"
                     /translation="MTDNGLQTASGGISCLPLKQLAAQLFKRVRYVSTHRTTHKAPPY
                     VSS"
BASE COUNT         1698 a         1879 c         2131 g         1974 t
ORIGIN      
        1 aggcgaggca acgccgtact ggtttttgtt aatccactat aattgaaggg gttatcggct
       61 tgtgcaacgg aagcccaagt tgtgcaagac atatttggat tgcaggctgg tacggatgcc
      121 gtagcggagg aaggcggcat agttggacga gtcgctcgac ccgatagacg cgccgctgca
      181 aaacatttgc gcgttggcat tgccggaaga aagcaggctg ctgttgaaat cttccgtcca
      241 ttcccaaatc agcccgtgca tatcataaac gccccagtag ttcgggcggc ctttgccgac
      301 atcgtgcagg cctttccgtc cgccgtcggc ataccaatcg agaatagtgc ggttgtagcc
      361 gggttcgttt gagccgtttt tctgcgtggc ggaagcaagt ccggcaaatt cccattcgtc
      421 aatggtcggc aggcgtttgc cttgtgcggc gcaataggcg ttggcggcaa accaggaaac
      481 attggttacc ggttgtttta attcgcccgc cttcggcgca tagctgcggc tgccgttttt
      541 catccaatgc ttcaggtaag cgggttctgc ctgtttggaa ccgatcctgc ctttttgcca
      601 ttgggggtgg ctgttgacaa attcggcaaa ctcggcattg gtaacgggat atttatccag
      661 tttgaacggt ttgactttaa tcaggccggt atcttttttc agataaagcg ggcggtagct
      721 gccgccttcg atttgaacca tttcggcagc cgccgcttga gtgccggcga gtgccgcaca
      781 gagaaagagt aaccggacat acttcataaa gcctcctgac aggcggttaa aatcaatctt
      841 ccgaaaggaa agattggttg ttaaaaaacc actgccgtgc gtaatgaagt acggcggcag
      901 tggttcgttt gcctaaaaat gggtgttcaa tttagtaatc gctttttgca ggagcagaag
      961 ctgcgggagc ggaagcagca ggagctgcac cgttaccggc gtaagcggta tcactcaatt
     1021 tttgagtcat gatttcaggg ttttctgcac cttctacttt caattgaccc agtgcgcctt
     1081 tgttgaatgc gcggaagata gagtggtcaa ccaaagtgta gctgcccggg atgtcgactt
     1141 tgaattcgac gatggcagag ccgccggcag gaacgatggt actttgtacg ttttcgttaa
     1201 tcagtttgcc gccttcaaca taaactttgt cgaagatttc accgattacg tggaaggaag
     1261 acaccaagtt cgggccgccg ttaccgacgt acatacgcac ggtttcgcct gctttggctt
     1321 tcagcgcgtt atcgccggcg atagaaccta catgaccgtt gaatacgacg tattcaggct
     1381 gttcggcaac ggctttgtcc atatcgaacg gttgcagacc ttgcgcgcct tttttgcctt
     1441 tggtgtagaa gtcgccttgg acgatgtaga actctttatc cactttcggc agaccttctt
     1501 taggctcgac caaaatcaga ccgtacatac cgttggcgat gtgcataccg accggcgcga
     1561 cggcgcagtg gtagatgtac aggccaggtt gcagggcttt gaagctgaat gtggaagtac
     1621 ggcccggagc ggtaaaggtt gcggccgcgc cgccaccttg gccggtagct gcatggaagt
     1681 caacgttatg gggaacggta gaagaaggat tgttagagaa ttctacttct acggtatcac
     1741 cttcgcgtac gcgaatcata cggcccggaa cgtcgccgtc aaatgtccag tagcggtatt
     1801 ccaccccgtc gtccattttc atggtttttt cgacggtttc catttttacg cgtactttgg
     1861 caggatagtc gcggtcgatt gcgggaggta cttcaggagc gtgggtggta actgcatcga
     1921 tgacgggcag ttcgcctgca ggcgtttcgg cggcagcttg tgcggcggaa cttgcggctt
     1981 ctgcggaagc ggcaggggct tgcgcggcag gttcgccgcc gcaggcggct aatgcgaata
     2041 aggaagcaat cattgcagct aaggcttggc gtttcataat gttttccttt tgtaagaaaa
     2101 gtagaggaat tggttaagaa cttggtgtga ttatgcgttt gccccattgg gggcattttg
     2161 atttaagtca aataattcat aaaatatgaa cttaaaatga tacttctaat actttatagc
     2221 tactaaaaat tacaataaat aggctgattt gcttaagggc ttcggcaaat caggcaaagg
     2281 cagggtagtt gccgactaaa gaataatgat agttattatc atatatgtat ttgttttata
     2341 tgtgagtttc tttgaacaaa tgtttatttg cgcggtaaac cgtgctacaa tctttaacat
     2401 tcatattctg tgaattttaa acaactgctt tattaaaagg agctctcaaa atgggacagt
     2461 acaagaagct gtggtacttg ctgtttgccg ttctggcggt atgctttacc attcttggct
     2521 atatgggcag cgaggtttat aagaaagccc cgccttaccc cgaacaggtc gtttccgcat
     2581 caggcaaagt gctgatgacg aaagacgata ttttggcagg tcagtctgcg tggcagagta
     2641 ccggcggtat ggaagtcggt tcgattctgg gtcacggcgc atatcaggct ccggactgga
     2701 cggccgactg gctgcatcgt gagttgtccg cttggttgga tttgaccgcg caacagactt
     2761 acggcaaaaa attcgatgaa gtttcccctg aagaacaagc cgttctgaaa acccgactgg
     2821 ctgacgaata ccgcaaccaa agccgtgtga aagaagacgg cagcgtcgtc atcagcgata
     2881 cgcgcgtgaa agccatcgaa agcatcctgc cttattacca cggcgtttac ggcgacgatc
     2941 ccaagctgca aaccacccgc gaacactttg caatgaaaaa caacacattg ccaagccaag
     3001 aagcgcgtga aaaactgttc gacttcttct tctggacttc ttggtctgct tcgaccaacc
     3061 gtcccggcga ggtctttacc tacaccaaca actggccgca cgagcctttg atcaacaatg
     3121 taccgaccac tgaaaactac atgtggtcgt ttaccagtgt cgtactgctc ttgatgggca
     3181 tcggtttgtt gatgtggggt tattccttcc tgaccaaaca cgaggaagtg gaagtgccgt
     3241 ctgaagaccc gatttccaaa atccaactga ccccttcgca aaaagcattg ggcaaatacg
     3301 tcttcctgac ggtcgccctg tttgtggtac aagtattgct gggcggtctg accgcgcact
     3361 acaccgtcga aggtcagggt ttctacggca ttgacgaggc gttgggcttt gaaatgtccg
     3421 actggttccc ctatgccctg acccgtactt ggcacatcca atccgccatc ttctggattg
     3481 caaccggctt tttgacggca ggtttgttcc tcgcaccgat tgtcaacggc ggcaaagatc
     3541 ccaagttcca acgtgcaggc gtgaacttcc tctatatcgc cctcttcatc gtggtcggcg
     3601 gttcttacgc gggcaacttc tttgcgttga cgcacatcct tccccccgaa tttaactttt
     3661 ggttcggaca ccaaggttac gaatacctcg atttgggccg tttctggcaa ctcctgctga
     3721 tggtcggtct gctgttgtgg ctgttcctga tgctgcgttg caccatctcc gcctttaaag
     3781 aaaaaggcgt ggacaaaaac ctgctggcaa tctttgtcgc ttctatggtc ggtgtgggcg
     3841 tattctacgc accaggcctg ttctatggcg aaaaatcccc gattgccgta atggaatact
     3901 ggcgttggtg ggtggttcac ctgtgggtgg aaggcttctt tgaagtattt gccactgccg
     3961 cttttgcctt tgtcttctac aatatgggct ttgtccgccg cagtaccgcc acagcttcta
     4021 ctctggccgc tgccgccatc tttatgttgg gcggcgttcc gggtacgctg caccacctgt
     4081 acttctccgg ctctacctcc gcctctatgg caatcggtgc ctgcttctcc gctttggaag
     4141 tcgtgccgct ggtgttgctg ggccgtgaag catacgaaca ctggtcttac caacacctgt
     4201 ccgactgggc gaaacgcctg cgttggccgc tgatgtgctt cgttgcagtc gccttctgga
     4261 atatgatcgg tgccggcgta ttcggcttcc tgatcaatcc gccgatttcc ctgttctata
     4321 tccaaggttt gaacacttcc gccgttcacg cgcacgccgc cttgtttggt gtgtacggtt
     4381 tcttggcatt gggcttcgta ttgctggttg cccgttattt gaaaccgaac gcgcagtttg
     4441 acgacaaact gatgacttgg ggcttctggc tgctcaacgg cggcttggtc ggtatgattg
     4501 ccatcagcct gctgcctgtc ggcgcgattc aggcatacgc ctccatcacg cacggtctgt
     4561 ggtatgcccg cagcgaagag ttcctgcaaa tggaaatcct cgataccttg cgttgggtac
     4621 gtacggcagc tgacttggtc tttatcggcg gtgcaatctg cgttgccatc caagctacta
     4681 aaatcgtatt cggccgcgac aaataagttt tgcggtatga aatgagcaac tgcacccgtt
     4741 ttcgggtgca gttttttatt gcggattggg acgggaatgg aaaaggatgt ggatttttta
     4801 ttttaatcgg ctatacaatt gctgctttca tgccgtctga agcacgtttc agatggcatc
     4861 ggtttacatc agataaggat aaaaatgggt atttacgatt ttcaaatgaa agatgcggaa
     4921 ggcaatgcgg ttgatttgtc aggctatcgc ggcaaggttc tgctgattgt caacacggca
     4981 acgcgttgcg gtctgacccc gcaatacgag gctttgcaga agctgtatgc acaatatacc
     5041 gcagaaggct tggagatttt ggattttccg tgcaaccagt tccgcgaaca ggctcccgaa
     5101 agcagcggag aaattgccca agtgtgtatg atgaagtttg gcacgaagtt taagattttc
     5161 gacaaaatcg aagtcaatgg agcaaatacc gcgcccttgt atgcctacct gaaatccgtc
     5221 aaaccgcaag ataaaggcaa tcatctgttt aaagatttcg tgttgaagct cgcagctttg
     5281 ggagagaagc gcgacgaagg cgacatcaaa tggaacttta ccaaattcct cgtcaaccgc
     5341 gacggcgaag tggtcgagcg ttttgcgcct agtgttacgc ccgaagaaat cgaagccgat
     5401 attcgggcat tgctgtaatc tgccgccgtc ttgggaaatg ccgtctgaag gcttcagacg
     5461 gcatttgtcc gtttgcgggt ttattccgaa atatcggcaa caccgctttc ggggaagatg
     5521 ggcaaatccg gcggtgggag cagggtatcc aaagtcaggg cggacgcatc gagtgtcggg
     5581 tagccttcaa acgcgaccga ataaccgctg tttccgttca tacggattcg ggcgtgcgcg
     5641 cctagaggga aagtgatttt gtcggcaatg tgtccgaacg ggaagcccgt cagcacgggg
     5701 attttcgccg tgcgcgaaac atggttggca acggcagaaa aatcataaga cggatcatag
     5761 acatctcgaa ttttttccat acggaaattg ccgaacacga tggcgcgctg tttcttcaaa
     5821 atacccgaaa gatacagcgt attgagcata cgttcgatgc ggtagggctg ttcgccgaca
     5881 tcttcgagga acaaaatgcc gccgtcgatg tcgggcatat aaggcgtgcc ggcgagcgag
     5941 gcgaggacgc ttaagttgcc gccccacaat atgccttcgg tttcgacatc ggcgcgttgg
     6001 atataaggaa catcaacggt caggcggttt tgggttgccc ccttgataaa ggcatccatt
     6061 gtaaacacgc tgggggagag tttgccgaat tcgctataag ccatcgggcc ggcaaaactc
     6121 atcatattgc cttttgccaa caatgccagt tggacggcgc aaacatcgct gaatccgaaa
     6181 aacagcgtgc cgtgttcgcg catccttgtg ccgagcgaag caaaatcgat atgcggcaga
     6241 atccgcgccg caccatagcc gccgcgcaag cccatcagca ctttaggtgt ggcgacgcgg
     6301 ccggaggcga cctcttggaa atcggcggca cgttgcgcgt ccgtaccggc aaaccgttgg
     6361 aaacggcggc tgcccgcctg ttggttggtt acggtaaaac cggcattgta aaggcgggtt
     6421 aaggctgtgt tgacgcggtt ggtgtcttcg gcaaatcctg acgaagcgac aacgcgcaga
     6481 aggttgccgt ccgaactttg acggcgtggc gtttggagag gcacggttcg ggctttcaca
     6541 acggaatggg aagagggaag gggcggaacg gatgtggcgg atgtgccgca agcctgaagc
     6601 agccccgcgc cggcagcggc ggtgcaggtt ttcagaaaac ggcggcggga ggtcggttcg
     6661 gtcataattt ccctttcata cggtttcaga cggcattggc gggcttaaag cagcttttta
     6721 agctgttccg tccaatcttg cggcagggtc ataccgtgtt tccggacggg gtttgcccaa
     6781 atcggcgcgg ggaaagacgc atcgttttca aagcgggcga taatatgcca atgcaggtgc
     6841 ggcacgacat tgcccaagct ggcgaggttg atttttgccg gccggaacac ttggcgcata
     6901 gcggcttcga ctttgtacac catttccatc aattcgccgc gttccgctgc cgaaaggtcg
     6961 gtcatttcgg cgatgtgttc atgccagata acacggcaga atgcaggcga accgctgtcg
     7021 ttatggacgg cgatgacgcg cagcttgggg gtttgcagca aaatatcttc atcttgtgcc
     7081 gtgcagatgg ggcagggttg ggcggtcatt tttccttatt cctgaaagtg tttaaaaaac
     7141 ggtagggtaa tttttaatat ttcaatctgt ctgtccgcat cgccgttaat tgtaccactt
     7201 catcgtgttt ccttttcggt tgaaaccccg tcctttgtgg cggcaggaac agactttatt
     7261 tgggaggggt gtaacccctc ccaaatcagg acgacacata gggcggtgct ttatgtgtcg
     7321 tcctgtgtgt tgaaacatat cggacgcgtt tgaacagttg tgcggcaagc tgttttagcg
     7381 gcaggcaaga gatgccgcct gatgccgtct gaagcccatt gtccgtcatt cccacacagg
     7441 cagttgctac ggttgctgaa gccccttagc cgtcgcgcag gcggttgcta cggttactaa
     7501 aatcccgtca ttcccgcgca ggcgggaatc cagtccgttc ggtttcagtt gtttctgata
     7561 aatcttgcag cgttgggttt ttagattccc gctttcgcgg gaatgacggg gcggtcggat
     7621 gccgcctgaa actccgtcat tcttgcaact tttcgtcatt cccgtgaaaa cgggaatcta
     7681 ga
//
LOCUS       DAWFJW010000093         7651 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFJW010000093 DAWFJW010000000
VERSION     DAWFJW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.093"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            154..327
                     /locus_tag="WGR19_001621"
     CDS             154..327
                     /locus_tag="WGR19_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0799119.1"
                     /translation="MKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            391..1674
                     /gene="hemL"
                     /locus_tag="WGR19_001622"
     CDS             391..1674
                     /gene="hemL"
                     /locus_tag="WGR19_001622"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0799120.1"
                     /translation="MNRNEILFDRAKATIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(1890..2160)
                     /locus_tag="WGR19_001623"
                     /pseudo
     CDS             complement(1890..2160)
                     /locus_tag="WGR19_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
     gene            complement(2171..2734)
                     /gene="orn"
                     /locus_tag="WGR19_001624"
     CDS             complement(2171..2734)
                     /gene="orn"
                     /locus_tag="WGR19_001624"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0799121.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESLHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2752..3639)
                     /gene="prmA"
                     /locus_tag="WGR19_001625"
     CDS             complement(2752..3639)
                     /gene="prmA"
                     /locus_tag="WGR19_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0799122.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETLEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLHLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGFAVGVDIDEQAVRAGKDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            complement(3900..5261)
                     /gene="accC"
                     /locus_tag="WGR19_001626"
     CDS             complement(3900..5261)
                     /gene="accC"
                     /locus_tag="WGR19_001626"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0799123.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKVCVVG
                     KDRDQAMAKMRVALAELAITGIKTNTPLHRDLFNDPGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5374..5829)
                     /locus_tag="WGR19_001627"
     CDS             complement(5374..5829)
                     /locus_tag="WGR19_001627"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0799124.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAPPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5811..6068)
                     /locus_tag="WGR19_001628"
     CDS             complement(5811..6068)
                     /locus_tag="WGR19_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0799125.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6185..7225
                     /gene="queA"
                     /locus_tag="WGR19_001629"
     CDS             6185..7225
                     /gene="queA"
                     /locus_tag="WGR19_001629"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0799126.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHAALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1601 a         1920 c         2279 g         1851 t
ORIGIN      
        1 acggaagagg gaatcgcaca aacggttgtt aaaggcaaaa cgacccgggc ggaggtagag
       61 gcacgtttcg ggaagcgcaa ccctttcggt tgttatgcct atcacgaggt cagcctgccg
      121 atttataatt ttttgccgac caatttcatt tatatgaaat cagaacgccg gcattgggag
      181 tggtgtgtgg attacgacgg ggagggagtc gtccgggact accgctttac acataaagtg
      241 gaaaaagacg agcgttccgt catccgggat gcggttggcg tgatccgcaa agaagcgggc
      301 aaatccttgt cgcaacctga aaaatgataa aatagctttt tctgtttcag aacctaaaac
      361 ctgcctttag gatggaattt aaggataaat atgaaccgta acgaaatttt attcgaccgc
      421 gccaaagcca ccatccccgg cggcgtgaat tcccctgtcc gcgcattcgg cagcgtcggc
      481 ggcgtgccgc gcttcatcaa aaaagccgaa ggcgcgtatg tttgggacga aaacggcacg
      541 cgttataccg actatgtcgg ctcttggggg cctgcgattg tcggacacgc gcaccccgaa
      601 gtcgtcgaag ccgtgcgcga agccgcgttg ggcggtttgt cgttcggcgc gcccaccgag
      661 ggcgaaatcg ccattgccga acaaatcgcc aaaatcatgc cgtccgtcga acggctgcgc
      721 ctcgtcagct ccggtaccga agcgacgatg accgccatcc gtttggcacg cggttttacc
      781 ggtcgcgaca aaatcatcaa gtttgaaggc tgctaccacg ggcattccga cagcctgctg
      841 gtgaaagccg gcagcggcct gctgacgttc ggcaatcctt cttccgccgg tgtgcctgcc
      901 gactttacca aacacacttt agtccttgag tacaacaaca tcgcccaatt ggaagaagcc
      961 tttgctcaaa gcggcaatga cattgcctgt gtgattttgg agccttttgt cggcaatatg
     1021 aaccttgtcc gcccgaccga agccttcgtc aaagccctgc gcgaattgac cgaaaaacac
     1081 ggcgcggtgt tgatttacga cgaagtgatg accggtttcc gcgtcgcgct cggcggcgca
     1141 caatcgctgc acggcatcat gcccgacctg accacgatgg gcaaagtcat cggcggcggt
     1201 atgccgcttg ccgcgttcgg cgggcgcaaa gacatcatgg aatgtatttc cccgctgggc
     1261 ggcgtgtatc aggcaggcac cttgtcgggc aacccgattg ccgtcgccgc aggcttgaaa
     1321 acgctggaaa tcattcagcg cgaagggttt tatgaaaacc tgacggcgcg taccgaacag
     1381 ttggttcaag gttttcggac ggcagcggat gcggcgggca tcgagttcac cgccgacagc
     1441 gtgggcggta tgttcggtct gtatttcgcc gcacacgcgc cgcgaaatta tgccgatatg
     1501 gcgcgttcca atattgaagg cttcaaacag tttttccacg ggatgctcga ccgcaatgtc
     1561 gccttcggcc cgtccgctta tgaagcaggt ttcgtttccg ccgcgcatac gcccgagctg
     1621 attgacgaaa cggttgcggt tgcggttgaa gtgttcaagg cgatggctga atgatgtttt
     1681 gacggacagg gtttctctgt tcgatctgtt tggcagattg aagtaagaat gcacaccgcc
     1741 gtcattcccg cgcaggcggg aatccagacc tttcagtttc tgtaatgatt gaaaataacg
     1801 ggccgaaatc agccgcgccc ggcaggaagc cgccgccacc tgaaacaatg ccgtctgaag
     1861 ccttcagacg gcattttatc ggaagcgcat cagaaaaaac ggatagcgac atatgcggta
     1921 aaagcaaggg caattacata gataaacccc tttataaatt caaaagtctc atctatatcg
     1981 ctatattttt ctttcagttc gtcagatttt cgataacgtt tcattttcaa gaccacttca
     2041 tgaccaacaa gcccgacctt ccggattccc gcctgcgcgg gaatgacggg cgtgtatatt
     2101 tttgatttca atctgctgta aaaatgccgt ctgaaatgca aacagcgttt cagacggcat
     2161 tgtttgtttc ttattgcgct tcggctctcg gggcggaaat cagaaagtgt tcgcggtagt
     2221 ggcgcatttc ttcgatgctc tccaaaatgt cgtccaatgc cttgtgcgaa ccgcgtttga
     2281 cgacgctttt ggcaacgggc ggattccagc gtttggcgag ttctttcagc gtggaaacgt
     2341 cgaggttgcg gtagtggaag tagttttcca gtttcggcat atatttgacc ataaaacgcc
     2401 ggtcttggtg gatggagttg ccgcacatcg gcgtggcgcg tccgggtacc cattccgaca
     2461 taaagtccag cagtttctgt tcgacttcgg cttcggtatg caacgattcg cgcacgcgct
     2521 gtgtcagccc cgtcctgccg tgtgtggcgg tgttccattc gtccatattg tcgagcagct
     2581 cgtcgctttg gtggacggcg taaacttcgg attgcgccaa tacattcaaa tccgagtcgg
     2641 taataatcat cgcgacttcg ataatgcggt cggtttcggg attaagcccc gtcatttcca
     2701 tatcgagcca gcagaggttg tttttgtcct gcacggtgtt tcctttccgt ttcagcgttt
     2761 tgtcccgctc aagcgcgccc agccttcttc ggtttccgcc gggtcgagat cgaaccattg
     2821 gctgtaaatg ccgccgagtt cttcggcctg ttcgtccaac aaaccggata acacgatgcg
     2881 gccgccttgt ttggtgcggg cggcgagcat ttcgccgagc atacgcaaag ggttggcgag
     2941 gatgttggcg acaactacgt cgaattgccc ttgaggcaga ccgtcgggca ggaagaattg
     3001 tgcatcgacg ttgttttgcg cggcgttgtc cttgccggcg cggacggcct gttcgtcaat
     3061 atccacgccg acggcgaaac ctgcaccgag tttgagggcg gcgatggtca ggatgcccga
     3121 accgcagccg tagtcgagga cgctttcgcc gtttttgagt tgcgtatcca accatttgag
     3181 gcagaggcgc gtggtcgggt ggctgccggt gccgaaggcg agtccggggt cgaggtggag
     3241 gttgacggca ctgccttcgg ggacttcgtg ccaagagggg gtaatccaca ggcggtcgga
     3301 aatccggatg gggtcgaatt gcgattgcgt gaggcgtacc cagtcttggt cttcgagggt
     3361 ttcgccggtg tatgccaagt cttttaaccc gcattcttgt gcggcgattt ggatgatggc
     3421 ggcggcttcg tcgtgttcgc cgaacagggc gatgactttg ctctgctgcc agatttgttc
     3481 ggcgggcata ccgggttcgc cgaaaatcgc ctgttcgttt tgcgtgccgg cgtaggcatc
     3541 ttcgatggcg gcggagagtg cgccgtgttc catcagcgcg tcggcgaggc gttcggcgac
     3601 ggcatcgttg acgttgacgg tgatttgttg gtagggcata acgggctttc ttggaaatat
     3661 agtggattga atttaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
     3721 aaccgattca cttggtgctt cagcacctta gagaatcttt ctctttgagc taaggcgagg
     3781 caacgccgta ctggtttaaa gttaatccac tatatcgaaa tcggtttcag acggcgcggg
     3841 gcaagggaaa tgccgtctga acgcggacgg gcggcttcag acggcatatc ggcggcggtt
     3901 tacttgtcct gtttggcttt gcgatcttcc agccagtgtt ccaaatagtg gatgctgacg
     3961 ccgccttttt ggaaacctgg gtcgttgaac aagtcgcggt gcagcggcgt attggttttg
     4021 atgccggtaa tcgccagttc ggcaagggct acgcgcattt tagccatggc ttggtcgcgg
     4081 tctttaccga cgacgcagac tttgccgatc aggctgtcgt agtacggtgg gatgcggtag
     4141 ccttggtaaa tgtggctgtc tacgcggatg ccgaagccgc cgggcaggtg gcagctttca
     4201 atcgggcccg ggctggggat gaagttgtac gggtcttcgg cattgatgcg gcactcaaac
     4261 gcgtggcctt cgacttgaat atccttttgt ttgtattgca aaggcaggcc ggcggcgatg
     4321 cggagttgct cctgcacgat gtccacgccg gtaatcagct cggtaaccgg atgctcaacc
     4381 tgaacgcgcg tattcatctc gataaagaaa aattcgccat cttcgtacag gaattcaaac
     4441 gtacctgcgc cgcgatagcc gatgcgtttg caggcgtcgg cacaggcgtt gccgattttg
     4501 gcgcgttctt tttcagtgat gaacggagcc ggtgcttcct cgatgacttt ttggtggcgg
     4561 cgttgcagcg aacagtcgcg ctcggcaagg tagatggcgt tgccgtgttc gtcggcaatc
     4621 acttggattt cgacgtggcg cggacgttgc agatagcgtt ccatgtaaac catcgggttg
     4681 ccgaatgccg cgcctgcttc tgccttggtc atttcgacag attggaggag gtcttctttt
     4741 ttctcgacca cgcgcatacc gcgaccgccg ccgccgccag aggctttgat aatgacggga
     4801 taaccgactt tatcggcgat tttgaggatt tcttcgccgt cgtcgggcaa tgcgccgtca
     4861 gaaccgggga cgcagggtac gcctgccgct atcatcgcgt gtttggcgga gactttgtcg
     4921 cccatcaggc ggatggtgtc gggtttcggg ccgataaagg taaagccgga ctgctcgacc
     4981 tgttcggcga aatcggcgtt ttcggcaagg aaaccgtagc ccggatggac agcgtccgcg
     5041 cagcttactt cggcggcggc gataatggcg gggacgttaa ggtagctttg cgcggaagcg
     5101 gcagggccga tgcacacgga ttcgtcggcg agtttgacgt gcaggctgtc tttgtcggcc
     5161 tcggaatgca cggcgacggt ggcaatgccc atttcgcggc aggcacggag tacgcgtaat
     5221 gcgatttcgc cccggttggc gattaaaact tttttcagca tgatgactgt tcctgtgatt
     5281 ccggcaaccc gtccgtaaaa aaaaggtgcg ggaacgccga agggggaaag catttcagac
     5341 ggcatcggaa gtttatgccg tctgaaaaca ggattatccg atgatgaaga gcggttcgcc
     5401 gaattcgacg ggcgtaccgt tttcgaccag aatttctttg accgtgccgg atttttcggc
     5461 ttcgatttcg ttcatcagct tcatcgcttc gatgatgcac agcgtgtcgc cggctttaac
     5521 ctgttggccg acttcgacaa aaggcgcggc attcgggccg ggtgcgcggt agaacgtgcc
     5581 gaccataggc gatttttgcg cgtcggacaa atcgcgggcg gcaggtgcgg cggcgggcgc
     5641 ggatgccgca acgggtgggg cggcaggcgt tacggccggc gcggcggcag gtacgggcgc
     5701 ggcgtaaacg ggtgcggcgg cgatggttcg ggtgatgcgg actttttcct cgccttcggt
     5761 tacttcgatt tcggcgatac ccgattcttc aaccaaatca atcagttttt ttaatttgcg
     5821 caaatccatt tctgttcctt taaaggctgc cggtgttccg gcgggttgtt gcgttggttt
     5881 ttcagaaaac agccggcttg cggcaacggc gcaaaccggg cggaatggtg ttcggatacg
     5941 gttttcctgt ggtggaaaac aaccgtgaga atgtgtttat tttcccgaag ttgtcggtaa
     6001 atgtccagta aaatatcaaa aaacggcggt ttttggggga aatgtgcagg aagcttgatt
     6061 ttgcgcacaa gatgccccgg gtcaagagtg tttattctaa tctgttggtt tttcggacaa
     6121 agatgccgtc tgaaaaaggg ctaaagtgcg tataatggcg gcttgcccga acgagagtgt
     6181 aaaaatggat atttcagatt ttgattttac cctgcccgaa aagctgattg cccagcatcc
     6241 gcccgaagtg cgcggcagca gccgcctttt ggttgcgttg cccgatatgc cgctgcaaga
     6301 ccgggtgttc ggcgatttgc cggattatgt cgaggcgggc gacgttttgg tgttcaacaa
     6361 caccaaagtc atgaaggcgc ggctgtttgg gcagaaagac agcggcggca ggatcgaagc
     6421 cctgattgag cgcgtgttgg acaaccatgc cgcactggcg cacatccgtt cgtccaagtc
     6481 ccccaagccc ggtatggggc tggtgtttga aggcggcatc cgtgccgtga tggtcgggcg
     6541 tgagggcgaa ctgttctgcc tgcgttttga aggcggtcaa accgtttacg aacttttgga
     6601 acagaacggc catttgcctc tgccgcctta tatcgaacgc gccgccgatg cggatgacga
     6661 cagccgttat caaaccgttt atgccaaata tcagggtgcg gtcgctgcgc cgacggcggg
     6721 cctgcatttt acggaagaac ttttgcaccg tctgaaagac aaaggcgcgg taaccgcaga
     6781 agtaactctg cacgtcggtg cggggacatt ccaacccgtg cgtgtggaca aaatcgaaga
     6841 gcataaaatg cacagcgaat ggtttgaagt gccgtctgaa accgccgccg ccgttgaggc
     6901 ggcaaaagcc cgggggaaca aagtctgggc ggtcggcacg acttccatgc gcgccctcga
     6961 atctgccgcg cgtgcgacgg gtcgtctgaa agcggggcag ggcgacaccg atattttcat
     7021 cacgccgggc taccgtttta atgttgccga caggctggtt accaattttc atttgccgaa
     7081 atcgacgctg ctgatgcttg tcagcgcgtt ttcgggtatg ggtcatatcc gcgccgcgta
     7141 tcgttatgcg gtcgaacgcg aataccgttt cttcagctac ggcgatgcga tggttttggg
     7201 gcggaacgaa ggggtggtac gttagaacag ccccaagtct attgggggaa gacaagtgcc
     7261 gtctgaacgg acgttcggac ggcattcttt tgttgatttg aatgaaagga actgtatgac
     7321 atagattcag gcgcgagagg cgagaaactc agtcattgtt gatgacgcgc aatacgttaa
     7381 aattcattcc gttttggtcg tataaacata ttacatacgc catttttttg tattcaaggc
     7441 tgcctgaaaa cagtgggcaa gaatatctaa cccaacgaaa agctgaaaaa gcaacaaaaa
     7501 ataatctgca ccttagtcgg ttggttagtc caaccttagg ggtgcagacc attcaatctt
     7561 tcaggctgcc tttgtttgtt tataagataa atttttagcc gaacactttt tccaagtgtg
     7621 cctgataatc tgccaagtat ttttccactt g
//
LOCUS       DAWFJW010000094         7546 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFJW010000094 DAWFJW010000000
VERSION     DAWFJW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.094"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            141..722
                     /locus_tag="WGR19_001630"
     CDS             141..722
                     /locus_tag="WGR19_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0799127.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(784..1638)
                     /gene="folD"
                     /locus_tag="WGR19_001631"
     CDS             complement(784..1638)
                     /gene="folD"
                     /locus_tag="WGR19_001631"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0799128.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1861..1938
                     /locus_tag="WGR19_001632"
     tRNA            1861..1938
                     /locus_tag="WGR19_001632"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1896..1898,aa:Pro,seq:tgg)
     gene            1963..2039
                     /locus_tag="WGR19_001633"
     tRNA            1963..2039
                     /locus_tag="WGR19_001633"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1997..1999,aa:Arg,seq:tct)
     gene            2067..2142
                     /locus_tag="WGR19_001634"
     tRNA            2067..2142
                     /locus_tag="WGR19_001634"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2100..2102,aa:His,seq:gtg)
     gene            2353..2859
                     /locus_tag="WGR19_001635"
     CDS             2353..2859
                     /locus_tag="WGR19_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0799129.1"
                     /translation="MVDAWSVPDFESKICPPESLAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2856..4634
                     /locus_tag="WGR19_001636"
     CDS             2856..4634
                     /locus_tag="WGR19_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0799130.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIYGLLNMIAAEGREYEHI"
     gene            4646..5512
                     /locus_tag="WGR19_001637"
     CDS             4646..5512
                     /locus_tag="WGR19_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0799131.1"
                     /translation="MKWLFILLVAINIAVFGGTVGYKLTLKQAGRIPEAQNAANNLQV
                     QPVAPTMPVVRNIPASGPVVQAASGSDTGVLLGASDILTEEQAEQLRLKKEAEQKKLR
                     EKKQREEKARREKLAAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHV
                     ASRSVEKRTAKAKPADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSR
                     ENAQALQNRLAGAGFGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKIT
                     SLYGKLNLKSCK"
     gene            complement(6276..7064)
                     /locus_tag="WGR19_001638"
     CDS             complement(6276..7064)
                     /locus_tag="WGR19_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0799132.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(7278..7472)
                     /gene="thiS"
                     /locus_tag="WGR19_001639"
     CDS             complement(7278..7472)
                     /gene="thiS"
                     /locus_tag="WGR19_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0799133.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         1629 a         1911 c         2241 g         1765 t
ORIGIN      
        1 tatgtatggc gtaaaggaag cgttttaacg ccgtattggg aaaatgtaaa gttctttatc
       61 gggatttctt taagtattgg cgttttcggt gtcgtggcgg tagaatcggg gtcagttaag
      121 aacaattatc aggaacggat atggaagaaa aaaacgacta caccgacgcg gcatccgaca
      181 accgcaacgg gcaggaaatt gaagtcggaa ttgccggcgc aggcgaccgg atacttgccg
      241 cgctgctgaa ccaattgttt acctttttgg ttttgttggt gccgtttgcc ggactgattg
      301 cttttgccat caagaatgaa ggcaggattg gcggcagtga ggaaatattc gggctgcttt
      361 tgggcatgac ttccttttgg gtaggactgg cgggcatttt ggtttatacc gtcgttcaaa
      421 tctactatat gagtcgggac gggcagtcat tgggtaagaa aatcatgaga atccgcgtgt
      481 tgaaaaccga cggccgcaat cccggttttg tcggcacggt tttggtacgc gaaatcgcat
      541 ggtcggtttt ggttgccatt attgccgccg ttatcggtct tgcagtaggt gacaacggag
      601 aaaacgccat caacctgctg gcattccttg ccaactttgt cctgctcttt atggtcaaac
      661 gcgaccgccg cacgctttac gacatactgg cggatacggt tgtcgtcaag ctgcccaaat
      721 aaacggacac ggcaaaatgc cgtctgaaag cctttcagac ggcattttta tcttcagaac
      781 cgctcaagca tcgtgcagtg aagccgcgtg cagggtgttt tccatcaatg tggcaatcgt
      841 catcggaccc acgccgccgg gaacgggcgt aatcatcgcc gcccgttctt ttgccgtttc
      901 aaattccacg tcgccgcaca ggctgccatc gtccaaacgg ttgatgccca catcaataac
      961 gaccgcgcca ggtttgatcc attcgccttt gacaaagttc ggaatgccta cgccgaccac
     1021 caaaatatcg gctccggcaa cctcgtctgt cagattttcg gttgcgctgt ggcagaccgt
     1081 taccgttgcg cgcgccagca gcagttccaa agcctgcggg cggccgacga tattcgacgc
     1141 gccgaccacg accgcttttt tccccttcgg atcaatgccg taagcttcca aaagcgtcat
     1201 cacgcccttg ggcgtacacg ggcgcatcag cggcattttg accgccagcc tgccgacatt
     1261 gtaaggatgg aagccgtcca cgtccttatc cggcgaaata cgttccaaaa ccgcctggct
     1321 gtcgaggtgc ttcggcagcg gtagctgaac cagaataccg tccacttcgg aatcggcatt
     1381 caggcggtcg accagtgcca gcagttcttc ctgcgatgtt gattcgggca gctcgtaaga
     1441 cagtgatttg atgccgcatt tttggcaggc agttttcttg ttgcggacat aaaccgcgct
     1501 ggcagggtcg cctccgacca aaaccacggc caggcaaggc atgtgcagat tgtcctgttg
     1561 gcgttgcgcc accgcttcgg caaccgcctg caggcgtttt tgcgaaactt ctttaccatt
     1621 gatcagttgt gccgacatat ccgtcctttt gttaaacagg tagaaaatgc tgaaaattat
     1681 cgcattttcc gtttgttttt acagcaggat gccgaaaata aatttgccct gaaataaaat
     1741 acggctgttt ggacggatta aatatttttt caaatcaaaa aactatattt ttcatcttgg
     1801 tgtcttgaaa ataaatattg acgcatcttt ccctgagttg tatagttcgc ttcttcaagt
     1861 cggggcgtag cgcagcccgg ttagcgcatc tgctttggga gcagagggtc gtgagttcga
     1921 atcccaccgc cccgaccaat caatcagcac gaatcggcat gagcgcccgt agctcaaccg
     1981 gatagagcac cgaccttcta agtcgggggt tacaggttcg attcctgtcg ggcgtgccaa
     2041 gaatttgatt gaaaaccgtt tttatggtgg ctgtagctca gttggtagag ccccggattg
     2101 tgattccggt tgtcgtgggt tcgagcccca tcagccaccc cagataaagg cccgtacttt
     2161 tcggtacggg tctttttccg tttgcacgta ctgtccgcac cgtcgtaccg gtgcggcggt
     2221 atgccgtctg aaagccgggc cggtcatacg gaaaccgctt cagacggcat ttgcgccgcc
     2281 ggctttgcct ttcgcatatg cttctgtatc atgtaggttt tctgtctttt atggtttttg
     2341 tcggaggctt tgatggttga cgcttggtct gtccctgatt ttgaatcgaa aatctgtccg
     2401 cccgagtcgt tggcggcgcg tttggcgttg ttgccgcgcc cgctggtgtt taccaacggc
     2461 tgtttcgaca tcctccacag ggggcatgtt acttatctgg cgcaggcgcg ttcgatgggg
     2521 ggcgcgttgg tgctggcgtt gaataccgat gcttcggtgc ggcgtctggg caagggcggt
     2581 gaccgcccgg ttaatccttt ggagaaccgt gccgccgttg ccgccgcttt ggaaagtgtg
     2641 gatttggtaa cgtggtttga cggggatacg ccggcggcgt tgattgaggc ggtcaaacct
     2701 gagattttgg tcaagggcgg cgattgggct gcggataaga ttgtcggtgc ggcagaaacg
     2761 ttggcgcgcg gcggtcaggt gttttcaatt ccgtttctgc accagacttc gacaacgaag
     2821 actttggcaa aaatccgtgc ggcagagggc ggaaaatgac ggttttgaag ccttcgcact
     2881 ggcgggtgtt ggcggagctt gccgacggtt tgccgcaaca cgtctcgcaa ctggcgcgta
     2941 tggcggatat gaagccgcag cagctcaacg gtttttggca gcagatgccg gcgcacatac
     3001 gcgggctgtt gcgccaacac gacggctatt ggcggctggt gcgcccattg gcggttttcg
     3061 atgccgaagg tttgcgcgag ctgggggaaa ggtcgggttt tcagacggca ttgaagcacg
     3121 agtgcgcgtc cagcaacgac gagatactgg aattggcgcg gattgcgccg gacaaggcgc
     3181 acaaaaccat atgtgtgacc cacctgcaaa gtaagggcag ggggcggcag gggcggaagt
     3241 ggtcgcaccg tttgggcgag tgtctgatgt tcagttttgg ctgggtgttt gaccggccgc
     3301 agtatgagtt gggttcgctg tcgcctgttg cggcagtggc gtgccggcgc gccttgtcgc
     3361 gtttgggttt gaaaacgcaa atcaagtggc caaacgattt ggtcgtcgga cgcgacaaat
     3421 tgggcggcat tctgattgaa acggtcagga cgggcggcaa aacggttgcc gtggtcggta
     3481 tcggcatcaa tttcgtgctg cccaaggaag tggaaaatgc cgcttccgtg caatcgctgt
     3541 ttcagacggc atcgcggcgg ggcaatgccg atgccgccgt gctgctggaa acgctgttgg
     3601 cggaacttga tgcggtgttg ttgcaatatg cgcgggacgg atttgcgcct tttgtggcgg
     3661 aatatcaggc tgccaaccgc gaccacggca aggcggtatt gctgttgcgc gacggcgaaa
     3721 ccgtgttcga aggcacggtt aaaggcgtgg acggacaagg cgttttgcac ttggaaacgg
     3781 cagagggcaa acagacggtc gtcagcggcg aaatcagcct gcggcccgac gacagaccgg
     3841 tttccgtgcc gaagcggcgg gattcggaac gttttctgct gttggacggc ggcaacagcc
     3901 ggctcaagtg ggcgtgggtg gaaaacggca cgttcgcaac cgtcggtagc gcgccgtacc
     3961 gcgatttgtc gcctttgggc gcggagtggg cggaaaaggc ggatggaaat gtccgcatcg
     4021 tcggttgcgc cgtgtgcgga gaattcaaaa aggcacaagt gcaggaacag ctcgcccgaa
     4081 aaatcgagtg gctgccgtct tccgcacagg ctttgggcat acgcaaccac taccgccacc
     4141 ccgaagaaca cggttccgac cgctggttca acgccttggg cagccgccgc ttcagccgca
     4201 acgcctgcgt cgtcgtcagt tgtggcacgg cggtaacggt tgacgcgctc accgatgacg
     4261 gacattatct cgggggaacc atcatgcccg gtttccacct gatgaaagaa tcgctcgccg
     4321 tccgaaccgc caacctcaac cggcacgccg gtaagcgtta tcctttcccg actacaacgg
     4381 gcaatgccgt cgccagcggc atgatggatg cggtttgcgg ctcggttatg atgatgcacg
     4441 ggcgtttgaa agaaaaaacc ggggcgggca agcctgtcga tgtcatcatt accggcggcg
     4501 gcgcggcaaa agttgccgaa gccctgccgc ctgcattttt ggcggaaaat accgtgcgcg
     4561 tggcggacaa cctcgtcatt tacgggttgt tgaacatgat tgccgccgaa ggcagggaat
     4621 atgaacatat ttaaggaata cagagatgaa atggctattt atccttttgg ttgcgattaa
     4681 tatcgccgta ttcggcggta cggtaggtta caaactgaca ctgaaacagg ccggcagaat
     4741 accggaggca cagaatgccg caaacaattt gcaggttcaa ccagttgccc caactatgcc
     4801 ggttgttcgg aatattccag catccggtcc tgtcgttcag gcggcatctg gatcggatac
     4861 cggcgtactg cttggtgcaa gcgatatttt gaccgaagaa caggcggagc agttgcgctt
     4921 gaagaaagaa gcggaacaga aaaaactgag ggagaaaaaa cagcgtgaag aaaaagcccg
     4981 ccgcgaaaaa ctcgccgccg aaaaggcgca ggcggaacgc gaaaacggcg cggcggatgc
     5041 cttatgcgcc gcgcaggcaa gcctcacgat ggacgaagat gactaccacc gcatcaaagg
     5101 acttttgggc aaatggtcgc acgttgccag caggagcgtt gaaaaacgta ccgccaaagc
     5161 caaacctgcc gacaaaacct atcgcgtcgt cctgcccgtt tccgccgatg ccgaaaatca
     5221 ggcggcggag ctgtctgcca aaggtttcaa ccccataccg tttgacggcg cattgagttt
     5281 gggtgtcggc aacagccggg aaaacgccca agccctgcaa aaccggcttg ccggcgccgg
     5341 attcggcggg gcgcatattg tcgaacactt tgccgaagcc gacaggcagg acgattcttt
     5401 gagcgtgtcg cgtatgacgg ttttgtttac cggcgtgaat gccgccgatg cggacgaaat
     5461 tcgtaaaatc acgtccctat acggcaaact gaacctcaag tcttgcaaat aggcgcgaaa
     5521 gccggacgat aaacgccaaa cccaccgcga aggtgggttt ggcgtttcgg ttttgagtat
     5581 ctgatatata aaaagggaat gtatgtctcg tatatgccga ctccaagtgt gaaagtgaat
     5641 tgtgagggaa tacagtgttt aatggttcgg aatggacgac ttccgagtgg ttttgattat
     5701 acgctaataa taataattat caataaaaat ttaaaaataa atgcggattt tttgtaatga
     5761 gatgaaaaat aaacaaatta aactatttgc cgatgtttgt gattcgggtc ggcaaacttt
     5821 cattgtgccg gacaaatcgg ggtcggcagg catagctgag ggcttcgtgc ctgacggaaa
     5881 taaggttgcc cgtgtttgaa catccgtccg atttggcgcg gacagggttg tcattcgggg
     5941 gtgtttgagc gtggattcgg ggatgcgaaa gcggtaaaac cgttgcggat gaaggcttac
     6001 ggacggcttt aggtattttt aagtgtattc aaacaacaac aaaacgcttc ttctattgcc
     6061 gtatttaggg cttgcgcggc agcctgcaca ccgtggaggc cgagtgtttc gttggttgcg
     6121 gcatcgggac gggcggcaag gttttccggc cggtttttcc attttttccg acaaagccgt
     6181 gatgccgggg aatggcggtt tgtccgtttc aggccgccgc cgttttggac cgcgatgccg
     6241 tctgaagcct tcagacggca tttttgctgc ctttttcaat attccgccga atgccaaaac
     6301 ggttgtccga ctgtcggcgt gctggcttgc gctttgtcgc gtgcttcgac cggtccggct
     6361 tcaaatgcca gccgtccgga ttcgacggcg agtgcgaagg cgcgtgccat attgaccgga
     6421 tcgccgctgc gggaaacggc agtattcaaa agcacgccgt caaagcccca ttccatcact
     6481 tgtgccgcct gtgagggcaa acccaagccc gcgtcgataa tcagcggcgt gtcgggcagg
     6541 cgttcgcgca ggacgttcaa cgcgtaggcg tgaaccgcgc ccaaacccgt gccgatcggc
     6601 gccgcccacg gcattaacgc ctgacagccc acatcgagca ggcggcggca ggcaatcagg
     6661 tcttcggtgc aataaggcag cactttgaag ccgtctttaa tcaggatttc cgccgcttcg
     6721 acaagttgga acacatccgg ctgcaaggtg tcgtcgtcgc cgatgagttc gagtttaatc
     6781 caatcggttt caaacacttc gcgcgccatt tgcgccgtcg ttaccgcttc ctgcacgctt
     6841 tggcagcctg ccgtgttcgg caggacggga acgccggttt cttgaagcag cgaccaaaac
     6901 ccctgaccgt gcgcctcgcc gccgcttccc gtgcggcgca gcgagacggt aatcatcgcg
     6961 ggttgggcga tacggacgga ttgtttgagg atttcagggg tcgggtaggc agccgtgccg
     7021 agcagcagcc gcgaggggaa agtttctccg tatagggtga gcataatggg ttcctttgta
     7081 tagtggatta acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga aaacgattct
     7141 ctaaggtgct gaagcactaa gtgaatcggt tccgtactat ccgtactgtc tgcggcttcg
     7201 tcgccttgtc ctgatttaaa gttaatccac cataacgttg ttttggggac aggggtcgtc
     7261 tgaaaaggca aaaccgccta gccgccgacc accggccgca cgatatcgat tttgtcgttt
     7321 tcgtttaaaa ccgtttccgc atacgcgcct ttggggacga aaacggtgtt gaccgccacg
     7381 gcaaagggct tttgcggcgc ggtttgggcg atgaggtcgg caacgcttgt gccgtgaagt
     7441 tcggcgggtt cgccgtttaa gatgatgttc atggtttctc gatgtttctg tatttatagt
     7501 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaaga
//
LOCUS       DAWFJW010000095         7363 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFJW010000095 DAWFJW010000000
VERSION     DAWFJW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.095"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            229..441
                     /locus_tag="WGR19_001640"
     CDS             229..441
                     /locus_tag="WGR19_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0799134.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            589..2172
                     /locus_tag="WGR19_001641"
     CDS             589..2172
                     /locus_tag="WGR19_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0799135.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILSLAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTIRRDESV
                     TVEVIESLNFRPARGETIDFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYVAAFVEHGAEAFVQ
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2254..3351
                     /locus_tag="WGR19_001642"
     CDS             2254..3351
                     /locus_tag="WGR19_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0799136.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3465..4580
                     /locus_tag="WGR19_001643"
     CDS             3465..4580
                     /locus_tag="WGR19_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0799137.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4705..5160
                     /locus_tag="WGR19_001644"
     CDS             4705..5160
                     /locus_tag="WGR19_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0799138.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5231..5707
                     /locus_tag="WGR19_001645"
     CDS             5231..5707
                     /locus_tag="WGR19_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0799139.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5695..6621
                     /locus_tag="WGR19_001646"
     CDS             5695..6621
                     /locus_tag="WGR19_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0799140.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWAWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6686..7243
                     /locus_tag="WGR19_001647"
     CDS             6686..7243
                     /locus_tag="WGR19_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0799141.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATHGLYQNVPVQPVKIRRIVVGQ"
BASE COUNT         1921 a         1815 c         1919 g         1708 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataaat gcgccgattg ccaaggcgat gatgctcaac
       61 cagggttttt gcgaagcgtt cattgttgtc gtcctgatgg gaattgccgc cattatgcgc
      121 aagcgtccgt ctttttccaa accgccgcac cgtttccgcg tttgcccaag ttcacgcccc
      181 gacttacaat aacgcccgtc agattgcagt tccaacgaaa ggaaaatgat ggaaaccctt
      241 atcctcgaca tcggcggcat gagctgcggc ggctgcgtca aaagcgttac ccggatattg
      301 gaaggcgtaa aaggcgtggc aagcgtcgaa gtcagccttg aaaacaaaag tgcgaccgtc
      361 ggatacgacc ccgcgcaaac cgatgcaggg gcgttgattg aagccgttga agacggcggc
      421 tatgatgcgg cgttgaaata aagcggcaaa aatgccgtct gaagccttcg cgcctccccg
      481 atgccgtctg aagtacgctc cgcgccttca gatggcattt gatttagttg aacgggtagg
      541 atgaattggt taaacttgcg ggcaatagtg ccttcagaca aacaaaatat gccgaacaaa
      601 aagaaaaaag ccgcctttgc gcgtgctgca acggagaacg gtgtgccgga cgtgtccaac
      661 atcaaaaatt catatgatga tttgatgtat gaatccaaag cattttccca aaccgccatc
      721 aacgccttgg aggctcgcgc ccgcatgatg ggtttgcagc ccgcgcccgc cgccaatgcc
      781 aaggtcttgg agctgggctg ttcgatgggc ggcaacatca tcacgcaggc tctttattac
      841 ccggatgcgg aatttgtcgg tatcgacttg tccggcaggc aggttgcgca gggcaacgcc
      901 atcattgaaa aaatgggctt gaaaaatgtg cgtctggaag aaaaagatat tttgaccgtc
      961 gatgagtcat tcgggaagtt tgactatatc atcgtccacg gcatttggtc gtgggtgcct
     1021 gacgcagtta aagacaaaat tttttcgatt tgccggaaca acctgaccga acacggcatt
     1081 gcctatattt catacaatgt ttaccccggc tggaaacggc aggagcagtt gcgcgaaatt
     1141 atgtactttg ccggcaggga tgtgcttgaa gaacccttgg aagcgcggac gcggaaaggc
     1201 ttggacgcgc tcaaggcgct ggcggaaatt ttggaaaacg acaagggctt ggacggcggc
     1261 ggcaaacttc cggcgattca aaaaatattg aatcataatt tttattacat cgcacacgaa
     1321 tatatggagg cgtttaacga cccgatttac gtcaacggct tcatcgaatg ggcaaaccgc
     1381 caccggctgg cgtatatcgg ggataccgat ttgcacgtgt cttttgtttc ttggatggcg
     1441 gagcatacgc gggagcggat tctgtcgttg gccgggggcg attatattgc caaagaattt
     1501 tatagcgata ttttatccga ccgccaattc cgccgttcgc ttttatgccg tgaggaagtc
     1561 ggggatacta tcaggcgtga tgagtcggtt actgtcgaag tgatagaaag tttgaatttc
     1621 cgcccggcaa gaggggaaac gatcgacttt gatgaaaacg ataccctgct ttcgggtata
     1681 cgcgatgtga tgaaaaccgg agaggcgttt aaaacggaag atgttgcgga aaatcttgcc
     1741 cgccgatttc ccggtctgga atttgaccgc atgaaaatca attcccagct tttattgcaa
     1801 accattctcg ggcgtttttc tgtttcatca gacaatgcgg gcaaaccatt ttttgaagac
     1861 cataaaacct atgtgccggc gcgttttaca gattacgtcg ccgcctttgt ggaacacggc
     1921 gcggaagcgt ttgtccagcc tgccaaccgt tacaacgaaa gcaccccctc attcgggtac
     1981 gggcatttgt acattatgcg gcaattgtcg cggccgacga gcaaacaggc attgattgaa
     2041 acggttgccg aaaacttgaa catcgtcagc accacgcccg acggtttgac attccatccg
     2101 cctgccgaag tgtatgtgga agaaatattg gcagacttgg cagacaggca ttttctcgtt
     2161 tcggcggatt gacgggcaag tcccgacggc aatccgtccg atatattttc aaacaggaaa
     2221 aacacacact gcgaaaagcg gtacacgaca tatatgaacc agacatttac cttgcccgat
     2281 acgcgcccgt atccccaaaa tccgattaag aaccacctgc tgctcaatgc ctaccagttg
     2341 gcgcacaatt cttcccaggc ttcgcgcaaa ctctcgtccg gccaacttca aaccgaaatc
     2401 aggggaatgc ttgagcaaaa ccactatatc aacctttccc tcgcgctgac gatgtcgccc
     2461 gatgccggaa cttatgccgc gctgctttcc agtgtgaacg cggtactcga ttgcgagaaa
     2521 gaaggcgaag tgcagtggtt cgccctgccg gtcgtgctgg tgtccggctg caagaaagaa
     2581 cgggcaatcg agatgaagct gccgacggag ggattgtttg cctgtttgca aaactatccg
     2641 cacctgcgcg cgttgacgca agagacgcaa tggctgcctt atcttgtaca ttcctccgat
     2701 ttgagtgcgg tcgcgccgga tgagtggtgg cgtgccaaac aaaataccga agcggcggcg
     2761 caacacttac gccgtttcgc cccgcgccct ttgcttttgc ccgaagggca gtccgtccac
     2821 gtcgtttacg cgctgggttt cggcagcggc aaagttcaga cggcattggg tcagaacctt
     2881 cttcaggcag gcctgccctt aatgcaggta tggcaggaaa atcttgcatc ggaaggcgtt
     2941 acgctgtttg ccaatccgct ttccccggat tctccggtac gcgcgctttc agacggcagc
     3001 cacacgcgcc aacgtatggc gatggatgtg tttgcggcaa acgcgatacg cgccgtccgt
     3061 atgcagagtc cgcgcgtcgg cgtggtcgct gcggcaaagg cgggcggaca gattttattc
     3121 gggtttaatg cgaccgacgg cgcgtttgaa gtcgtgccgc aggtgttttc gtggcagctt
     3181 tcctttaccg acaacatcgc cgtcatccag caaaatttcc tcgacctgat ggcggagtgc
     3241 cgcgtggaac acgtttacct gttgcacaat cccttgggcg aacaggaaag catcccgagc
     3301 tatgcggaag cattgaaacg ggaagggcac aatccgtttt tcagcgcata gtgatttgta
     3361 gtagtgatag gctttctcat ttatataata aatcaaaata aagagcacaa cactttttca
     3421 ttctgtgttg tgccttgagt gaaacgaaag gatgaattat aaatatgaag attgtaattg
     3481 caccggattc gtttaaggaa agcttgacag ctcaacaggt agctgaagca ataaaaagag
     3541 gcttccaaca atcgatagca gatgtggaat gtctcctctg tcctgtcggt gatgggggag
     3601 aaggcactgt agatgctatc cgacattctc ttgacctaga agaaaaatgt ctccaagtga
     3661 caggaccttt tggacaaaaa gaagtcatgc gctattttca aaaagaacaa ctagccctat
     3721 tcgaagttgc tgatttggtt ggtcttggaa aaatcccgct agagaaacga aatccattac
     3781 aaatccaaac tcgtggtatt ggagaattga ttcgccacct cattagtcaa gaaattaaag
     3841 aaatctatat tggcgttggc ggtacggcca gtaatgatgg aggtattggg attgctgctg
     3901 gtttaggtta tcaattttat gatgaggatg gaaatgcctt acccgtttgc ggtcaatcct
     3961 tactaaacct agcttctgtt tcaacagaaa atcgctatga aattcctgaa gatgttcaca
     4021 ttcgtatttt agcagatgtt gtgagtccct tatgtggaca ccaaggtgcg acctatacgt
     4081 ttggcaagca aaaagggttg gattctacta tgtttgaggc cgtagatcag gcaatacaag
     4141 atttttatga aaaagtctcc cctgcaacat taaaacttaa aggagcagga gctggtggag
     4201 gcatcgctgg tggtttgtgc gcctttgctc aggcaagtat cgtatctgga attgacacct
     4261 gcctggactt gattgacttt gataagaaag tgtcagatgt tgacttggtt atcgttggtg
     4321 aaggaagact agatcgtcaa agtttagcag ggaaagcgcc tattggtgta gcaaaaagaa
     4381 cccctgtcgg agttcctgtt gtcgctattt gtggcagtct cgttgaagat ttgccttccc
     4441 tgccatttga aaatatccaa gctgcctttt ctattttgga gaaaagtgaa cctttagaag
     4501 atagtttgaa aaatgccagc ctctatttgg agcatacggc ttctaatatt ggacacttat
     4561 taaatatgcc taagatttag ccaaaccatt tcttccagat ggatgttttg gctggttctg
     4621 ccttatgcgg ttacgattaa ccgaccgatg ccgtctgaaa gggtttccgc ttcagacggc
     4681 atcgcgccgg aacagggagg ggatatgaaa aaaccgaaaa tcctttttgt ctgcctcggc
     4741 aacatctgcc gttcgccgat ggcggaatac attttgcgcc gccgcgccgc cgaagcgggc
     4801 attccccttg aagcggacag cgcggggaca tcgggctggc acgacggcga agatatgcac
     4861 cgcgagacgg caaagatatt gaaaaaacac ggtatcgatg cttcaggctt taccagccgc
     4921 aaaatccgcc aaagcgatgc ggcggcgttt gactgcatca tcgcgatgga cggcaagaat
     4981 ttgtccgaat tggaaagaac cttcggcagg cggccggaaa aaatattcaa gctgaccgac
     5041 ctgatacccg aaagcggtta cgaccatgtc cccgatccgt ggtatacggg cgattttgaa
     5101 gaaaccttca ggcttgcgga tgcgggctgt cgggcattgt tggaaaagat ttccaaataa
     5161 agcaattgaa tacaaatata aaaccgcctc ctgccgtatc ggttgttcag acggcataaa
     5221 cagagaattt atgaaaacaa atttcaaaca gaaaattatc gaacaggcac gcagcgaagg
     5281 cttgcaggta accgctttgc gcgagcaggt attagatatt gtcttgcagc aaagcggcgt
     5341 gattaaagcc tacaacgtct tgtcgcagat gcagcagcaa agcgagggcg tgcttgcacc
     5401 gcctaccgcc taccgcgccc ttgatttttg ggcggagcag ggcgttttgc acaaagtggc
     5461 ggcggtcaac ggctatattt tgtgcagcca cgcgcagcac gagtgcgacg accattgcca
     5521 cgaccacgaa gaagccgaag cgcaccacag cgcgtttatt ttggtctgca ccgaatgtgg
     5581 cacggcggac gagcaaaccc tgagccacga gtgggcggca ctgcgcgcag gcgttgccga
     5641 aagcggcttt gcgctgaaag aagaacacgt tgttttaacc gggatttgta aaaaatgtca
     5701 gaaatgaaaa aaaccaaagt ccacctgatt tcaggttttc tgggaacagg caaaaccacc
     5761 gcgctcaaaa gcctgatggc gcagaaagac ccgaacgaaa aatgggtcat catcgtcaac
     5821 gagttcggcg aaatcggcat tgacggcgcg gtattgagcg acaacggcat ccctgtggca
     5881 gaaatcgccg gcggctgttt gtgttgcacc gccggcccgc aaatgggcgt aaccgtgcag
     5941 aaaatgctgc gcgacgccaa gcccgaccgc ctgatgattg aagcaagcgg actggctcac
     6001 gcagccagcg tcatcgacga actcaaagcc aaaccgctgg acagcctttt ggaaatcggc
     6061 gccgtcttta ccgtcgtcaa tccgcgccag ttcatcaacc ccgattacgc gcaacaggcg
     6121 ttgtataaag accaaatcgg catctgcgac gtattggtcg ccagcaaaac cgatttatgc
     6181 acccccgaac agcttgccga atttcacgac aaagcggcaa aactgttccc gcccaaagcc
     6241 aaagtggttg aagtccaaaa cgcacaactc gacatccggt ggctcgatat tcccgtcatc
     6301 gaaaaatcac gctaccgcct caaagccctg ccggataata ccatgggctt ccagtcgcaa
     6361 ggtttcacat tccccgccgg acgcgatttc gacggcgaaa agctgaccaa ctttttcaac
     6421 gacctgccca aaatgaccga aggactcgtc cgcgccaaag gcgtgtttca agtattggga
     6481 acgtgggcgt ggctcaactg ggtagacgga cagtggggcg cgaaccaagt ctcatggcgg
     6541 cgcgattcgc gttttgaatt gattgccaaa tcgtttgatg cggatttaat cgaacaaaag
     6601 ctcaaagacg cattggaata gcgttcacgc gccttcagac ggcatatgaa atgaaaatgc
     6661 cgtctgaagg tctgaggagg acgggatgaa acccaaattc aaaaccgttt taaccgcgct
     6721 gcttttggcg gtttccctgc cgtctatggc ggcaacccat gttttgatgg aaaccgatat
     6781 gggcaatatc cgtttggttt tggacgagtc caaagccccc aaaaccgttg ctaatttcgt
     6841 gcgctatgcc cgaaaaggct tttacgacga caccgttttt caccgcgtta tcgacggttt
     6901 cgttatccag ggcggcggat ttaccgaaga cttggtacaa aaggcaaccg ataaggccgt
     6961 tgccaacgaa tccggtaacg gcttgaaaaa cactgccggt accatcgcca tggcgcggac
     7021 ggccgatccg gattccgcca ccagccaatt ctttatcaat ctggcggaca atgattcact
     7081 caactacaaa aacggacaat acggctatac cgttttcggc agggtcgaaa gcggcatgaa
     7141 caccgtttcc aaaatcgccc gcgtcaaaac cgccacgcac ggcctttatc aaaacgtacc
     7201 cgtacagccc gtcaaaatcc gtcgcattgt tgtcggacag taacacgcag acagatgttc
     7261 agacggcgtc gcccgtttca caaaaaacgc cgtctgaagt gaaaaaatat tttaaaacag
     7321 atagttgata ttgacaaatt caaaccgagg attttaaaat gct
//
LOCUS       DAWFJW010000096         7294 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFJW010000096 DAWFJW010000000
VERSION     DAWFJW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.096"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..322
                     /locus_tag="WGR19_001648"
     CDS             <1..322
                     /locus_tag="WGR19_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0799142.1"
                     /translation="LFAYTSDHGQYVRQDIYNQGTVQPDSYIVPLVLYSPNKAVQQAA
                     NQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGDAGSLSIHDGKAEY
                     VYPQ"
     gene            388..3864
                     /locus_tag="WGR19_001649"
     CDS             388..3864
                     /locus_tag="WGR19_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ0799143.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQDSSGHIGANTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRQPPTQEQLRPSESQGDQDLAAKLKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRAMLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TRLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYAAQQTTPSPVGEGRGEGKTVAAQTNFS
                     ATAANPLPNPLTQEREQSAAASTISDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFS
                     ATSTNPLPQEREQSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATTA
                     NPLPNPLPQEGEQSAAASAVSNDPQPKKQPAPQKNRLKPLPLADIRTFQAWLKTAERD
                     NPRLLFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTMTVPLT
                     VLNRLHAPSLEWLVPGMIREKIQLQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPI
                     LPQLAQAIAKTAGDIRIFEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHEL
                     QQQLGQAAAVTFRDNTQEFERDNVTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGR
                     IALRLFDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLR
                     DDLTQAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAVYAELN
                     SKLGKHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDA
                     AREADIQELEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSV
                     KRLLKEWEKIEK"
     gene            complement(3897..4988)
                     /locus_tag="WGR19_001650"
     CDS             complement(3897..4988)
                     /locus_tag="WGR19_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ0799144.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEAKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(5004..5648)
                     /locus_tag="WGR19_001651"
     CDS             complement(5004..5648)
                     /locus_tag="WGR19_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799145.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            6005..6217
                     /locus_tag="WGR19_001652"
     CDS             6005..6217
                     /locus_tag="WGR19_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799146.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWVWLKLDSDLYIKDESCDCPYV"
     gene            6514..6669
                     /locus_tag="WGR19_001653"
     CDS             6514..6669
                     /locus_tag="WGR19_001653"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0799147.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(6759..7208)
                     /locus_tag="WGR19_001654"
     CDS             complement(6759..7208)
                     /locus_tag="WGR19_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0799148.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         2118 a         1993 c         1478 g         1705 t
ORIGIN      
        1 gctgtttgcc tatacctccg atcatggcca gtatgtgcgc caagatatct acaatcaagg
       61 cacggtgcag cccgacagct atattgtgcc gctggtgttg tacagtccga ataaggccgt
      121 gcaacaggct gctaaccagg cttttgcgcc ttgcgagatt gccttccatc agcagctttc
      181 aacgttcctg attcacacgc tgggctacga tatgccggtt tcaggttgtc gcgaaggctc
      241 ggtaacgggc aacctgatta cgggcgatgc aggcagcttg agcattcacg atggcaaggc
      301 ggaatatgtt tatccgcaat aagcggcgtg aaaaccaata aagacaaatt tagataatgt
      361 cggggaagat gcccgaccga caagactatg caaaatatga aaaaccaagt acgcggatca
      421 ggcatggatg cacgatccaa cccagccaat gtttcagacg gcctgcaaga cagttcgggc
      481 catatcggtg ccaacacgcg ctaccgcctg accaaactcg gcgaacagat ggcgcgcctg
      541 cccatcgacc cgaaaatcgc gcgcattttg ctggcggcga agaaacacga ctgcatggcg
      601 gaaatattgg tgattgcgtc cgcgctgtcg attcaagacc cgcgcgagcg acctttggaa
      661 gcgcgcgatg ctgcggctaa ggcgcatgaa cgctttaccg acaagcagtc cgatttcctt
      721 gcctatctga acatttggga cagcttccag cgcgagcgcg acaaaggttt gtccaacaag
      781 cagctggtgc agtggtgccg ccaatatttt ctgtcgcacc tgcgtatgcg cgaatggcgc
      841 gagctgcacc accagcttgc ccaaactgcg attgaaatgg gtttgaccac gaaagaagcc
      901 gcgtttaggc aacctcctac ccaagagcaa ttaaggccgt ctgaaagcca gggcgaccaa
      961 gatttggcgg ccaagctcaa acaaaaacaa ctggacaaga aacaacaccg cgcccaaatc
     1021 cgtgccgcca aagaagccgg ctacgaacaa atccaccgcg ccatgctgac cggccttatc
     1081 gccaacgtcg gcatgaaatc gcccgacggc aacgactaca ccggcgcgcg cggcagccgc
     1141 ttccaccttt tccccgcctc cgccctgttc aaagccaaac ccaaatgggt gatggcggca
     1201 gaattggttg aaaccacgcg cctttacgcg cgcgacgtcg ccgttatcca gcccgaatgg
     1261 atagagcagg aagcgccgca cctcgtccgc tatcattatt tcgagccgca ttgggaacaa
     1321 aaacgcggcg aagtcgtcgc cagcgaacgc gtgacgcttt acggtctgac cgtattgccg
     1381 cgccgccccg tgtcttacgg caaagttgcc cccgaagaag cgcgcgaaat ctttatccgc
     1441 agcgcgttgg tggcgcagga atgcgatttg aaagcggatt tttttgtcca caacaaaaag
     1501 ctgattaaag aaattaccga actcgaacac aaatcgcgca agcaagacgt gctggtcgat
     1561 gacgaagccc tgtttgcgtt ttataacgaa cgactgcccg aaatggcttg gaaagacgcg
     1621 caaggcagcg tttggggaag tgaagattcc gtacggatta ttgaatctga caaagccgag
     1681 aggtcgtctg aaaatgagcg caacgagttt cgtaaaaaca agcgtaatgg gtctcgccaa
     1741 aatgaaaatc acggcaacac cgtaggttgg gttgaaaacc caacatcagc cgcaactgca
     1801 aaaactgttg ggtttgacaa tccaacctac gctgcccaac aaaccacccc ctcccccgtg
     1861 ggggagggtc ggggagaggg caaaacagtt gccgcacaaa ccaacttttc cgcaaccgca
     1921 gcaaaccctc tccctaaccc tctcacgcag gagagggaac agagtgccgc agcttcaacg
     1981 atttcagacg acctgcgtcc tgcaaatctg cagcaaaccg ccccctcccc cgtgggggag
     2041 ggctggggag agggcaaaac agttgccaca caaaccaact tttccgcaac ctcaacaaac
     2101 cctctcccgc aggagaggga acagagtgcc tcagcttcaa cgttttcaga cgacctgcgt
     2161 cctgcaaatc tgcagcaaac cgccccctcc cccgtggggg agggctgggg agagggcaaa
     2221 acagttgcca cccaaaccaa cttctccgca accacagcaa accctctccc taaccctctc
     2281 ccacaggagg gggaacagag tgccgcagct tcagcggttt caaacgaccc tcaacccaaa
     2341 aagcagcctg caccccaaaa aaaccgtctg aaacccctac ccctcgccga catccgcacc
     2401 ttccaagcct ggctcaaaac cgccgagcgc gacaatccgc gcctgctgtt cctcagccgc
     2461 gacgatctga tgcaacacgc cgccgcacac attaccgaag aacagttccc caaattctgg
     2521 caaaccgcag acggcaaatt caaactttcc taccgcttcg agccgcacca tccgctagac
     2581 ggcgtgacca tgaccgtgcc gctgaccgtc ctcaaccgcc tgcacgcgcc gtcgctcgaa
     2641 tggctggtgc ccggcatgat acgcgaaaaa atccagttgc aaatcaaagc actgcccaag
     2701 caaatccgcc gcatctgcgt gcccgtgccc gaattcatca cccaattttt aagccaaaac
     2761 cccgaccgca acgcccccat cctgccccaa ctcgcccaag ccatcgccaa aaccgcaggc
     2821 gacatccgca tattcgagca aatcaaccaa gacgaatggg ccgcgttcag gctgcccgaa
     2881 cactgctatt tcaacctccg cattatcgac gacggcggac aagagcttgc cggcggccgc
     2941 aaactgcacg aattgcaaca acaactcggt caagccgccg ccgttacctt ccgcgacaac
     3001 acccaagaat ttgagcgcga caacgtcacc gcatgggaca tcggcaccct gcccgaatcc
     3061 atcaaattcg cacgcggcaa acaacagctc accggctatc tcggcctaca aaaagaaaaa
     3121 gacggccgca tcgccctgcg cctgtttgat accacagaag ccgcagagca ggcacaccgt
     3181 caaggtgtca tcgaattgat gaagctgcaa ttaaaagagc aggtaaagga tttgaacaaa
     3241 ggcatccaag gcttcaccca agctgccatg ctgctcaaac acatcaacgc cgacactctg
     3301 cgcgacgacc tcacccaagc cgtctgcgac cgcgccttta tcggcgaaga cgagctgccg
     3361 cgcaacgaaa aagccttcaa agaacaaatc aaacgcgccc gcagccgcct gcccgccgtc
     3421 aaagaagccc tcagccgcta cctgcaggaa accgccgccg tctacgccga actcaacagc
     3481 aaactcggca aacacccatt gacccacctt ctaagactac gcctgcaaac cctgctcgcc
     3541 gccggcttcg ccacccgaac cccgtgggca caatggccgc gcctccccat ctacctcaaa
     3601 gccatgaccc tgcgcctcga aaaatacagc agcaaccccg cccgcgacgc agcccgcgaa
     3661 gccgatatcc aagagctgga acaaatgtgg caggaaaaaa cagacagcct gattaaacaa
     3721 ggtctcccca tttcagacgg cctcgccgcg tttaaatgga tgattgaaga attgagggtg
     3781 tcgctgttcg cgcaggaatt gaagacaccg tatccggtgt cggtgaagcg gctgttgaaa
     3841 gagtgggaaa aaattgaaaa ataaaaaaac agcctgaaaa gtttcaggct gtttttttat
     3901 ttgactaatc gaagtttcct atatctattt aagtccctct caactaatcc aaaagttaaa
     3961 tcagcaacat ctttggggga tacgtttaaa ttttcagcaa tctgttcaat accaatgcca
     4021 tcatttttta aaatagtaag cattttacgt aatgcgcttg atatttccct ttccattggc
     4081 tctggttcga tagttcgata ttttttcttt gcaaacaaag gacaaagatt gtgtatatac
     4141 atcctatcag taatcattcc taatttatgc atccgatatg ctaaggcaac aagtgataca
     4201 ccaaatcgtc ttttgatttt taataaattt tcaatagtga taggaacatg acgatataag
     4261 cgtagtgcag cctccggcat taaaaaagct gaagcaaagg cattagcctc tttttcgata
     4321 atatcacgag gttcatcttc tgtaatttca ctatttttac tatgttccat actgtattta
     4381 tcacggatta agtgccctaa ttcatgggca gcatcaaatc gactacgttc tgcagatttt
     4441 tgtgtattta aaaatacaaa tggatgattt tcataccaag tacaaaaggc atcaatgtcc
     4501 tttgtatcta aagataatga aaatacacga acacccttag cttcaagtag ggtgatcata
     4561 ttcggaatag gttcattgcc aagcccccat tctaatctta gttcctgagc agcctcttca
     4621 ggagaaatat cagaaaaatc aggcaatacg gcttgactta gtgtaaattc tgtctcgagc
     4681 cagtcattta acaaaaaagc cgtaatgcta tgatttaatg cttgtttttc aagcctcttc
     4741 gaggtgcgtg aacgagcacg aaaacttact gcctgagatt tcaactcagg cagtctttcg
     4801 tcattagtaa agaaatgaac tggaaactct aataaattgg ctaattcatt taaatcaggt
     4861 atttgctcat cttttacata gtttctaacc tgtcgagcgg taatacctaa taactcagct
     4921 aattttgttt gcgtacaacc acgtttatcc agcgcaaatt ccagtctctc acgattaaat
     4981 gtctgcatga tttatcattc aaattatcct gctttttgtt ttctttcaac caaaggctca
     5041 tattcttcaa caggttgctt acgttcaagc tcatcaaatt tagttaaatc aacatcagct
     5101 aatataattc gctgcttgta cccagttatt tgatgactaa caaaaccact cggtaaagat
     5161 aattcaagtt gcactttatt atacttccag tgaaacagca gaacccaaaa ctgcacagta
     5221 tcaggcaaat ctagttttga attacgaata gcctcctcaa atcccttacc tttccttgcg
     5281 gttgtcattg gcatcccgtg atgcctacca acatctgaag tagcagtagc cacaataata
     5341 cttttagttc gacatggcga gagacataga aatgcaccac ccgaccaagg ctcaagcgtc
     5401 cagccatctt tacttaaata tacccttaga gcaaatgtaa tttctgcttg ccgatacatc
     5461 cccaatgtat ttctgtcaga taatgctgct ttgtcctgaa tattattatg cgcagtaagt
     5521 acaatctctt taagcatctc ctgagataag tacttgctga tttcacttaa agcaatatca
     5581 ctattttgtt gctcgactat ttctcctact tcaaatggga aaggttctga taatgcaaat
     5641 tccaccataa aaatttccta attttatacg taatgtttac acaatatatc aggaaatatg
     5701 aaaacgtaca actatatcta taaagcaatt aataagtagc ctgcccaacc gtgtccttat
     5761 ctttcggcac acccgacctg caaatcacgc aaaacttgga atccgtgtgt agggtgtgtg
     5821 cggtacatac gcacgcagtc tttttaaacc acagcccttc ccaactaaac caaaaggtcg
     5881 tctgaaccct attttcagac gaccttttgc cactttgtaa aacaaatctt cccaccatcc
     5941 tctccccaaa catcgcccga accagtaaac ttctcatatt tcaacaactc cttggaagca
     6001 aaccatgtct ggtatctacc taccccgcct attcccgccc catatcgccg aacgcggcct
     6061 gttgtatttt cagcagggca aggttctcga tgtccgaaaa acttccgccg ggcattatcg
     6121 ggcggaggtg tgcggttcgg aaaactattg ggtatggctg aagctggata gtgatttgta
     6181 tattaaagac gaaagctgcg attgccctta tgtctaaagg tgcaaacata ccttaaatta
     6241 ctatattaca taggcaaaat acaagcctat aacgaattgg aaacaaaatg ccgtctgaaa
     6301 gcattataaa atctgttcac cttttcagat gagtaatgta cacccctata caatttttgc
     6361 tactataccc cataaatcca cggctaaaga tatccttatt atgtcctatg attgatcgaa
     6421 acgacttgta atcggcttag catcaagtgc cctattcgac ttatccgaat cggataatat
     6481 atttagaatg gaaggggcag aaacctatag gcaatatcag agagaaaaac aaaaccatcc
     6541 cctaaaaaag gcgttgtctt tccatttatt aaaaaacttc tgtcaatcaa tgaaataaac
     6601 ccaaacgacc caacgattgg gtttattctt ttatccagaa acaatccaga tacagattac
     6661 gagtcataac tataggctta atattacacg attctcattc catcaaggcg gaaaaccgca
     6721 caaatactga aacactatcg atcgatttgt aaacaagcct acttaagtaa cttgcagtcc
     6781 ttatcatttc ctttaaaata atccagcccg tcactacacg aactggcgga cttcttgcaa
     6841 ataaaggtta ctagattttc attcatctta ataataaaag gatttttatc tttatctatg
     6901 gctaccgcct tcaacatgaa tttactgtct aaagccccgc gcgcgattcc attcaaacgg
     6961 atacaaaagc cttctgcctc tttaatcggc aaacttggcc acttggtaga tgtttgttta
     7021 aacctcccat tctgcagata aaacttttcc ataaaatgtg cattttctaa caaggctgcc
     7081 cgcactgcat ttatctttgc tttctcaaca taattgcgat agctcggata aacaattaaa
     7141 gcaagtacag acaatatcaa gaccactgat attaattcaa ccagcgtaaa cccccgatta
     7201 tcagtcatta ctttacttcc aataagaaca gattattcaa catatttctt tgaacagact
     7261 tactatccca ttcaacagta tgcatatttc ccac
//
LOCUS       DAWFJW010000097         7162 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFJW010000097 DAWFJW010000000
VERSION     DAWFJW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.097"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            33..404
                     /locus_tag="WGR19_001655"
     CDS             33..404
                     /locus_tag="WGR19_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0799149.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QKRVQTLFSNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            401..1039
                     /locus_tag="WGR19_001656"
     CDS             401..1039
                     /locus_tag="WGR19_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0799150.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(1374..1450)
                     /locus_tag="WGR19_001657"
     tRNA            complement(1374..1450)
                     /locus_tag="WGR19_001657"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1414..1416),aa:Pro,seq:cgg)
     gene            complement(1531..2616)
                     /gene="nagZ"
                     /locus_tag="WGR19_001658"
     CDS             complement(1531..2616)
                     /gene="nagZ"
                     /locus_tag="WGR19_001658"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0799151.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(2673..4193)
                     /locus_tag="WGR19_001659"
     CDS             complement(2673..4193)
                     /locus_tag="WGR19_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0799152.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRTKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(4393..5892)
                     /locus_tag="WGR19_001660"
     CDS             complement(4393..5892)
                     /locus_tag="WGR19_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0799153.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEASFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(6030..6659)
                     /gene="nth"
                     /locus_tag="WGR19_001661"
     CDS             complement(6030..6659)
                     /gene="nth"
                     /locus_tag="WGR19_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0799154.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPIADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(6705..7118)
                     /locus_tag="WGR19_001662"
     CDS             complement(6705..7118)
                     /locus_tag="WGR19_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0799155.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         1561 a         1827 c         2135 g         1639 t
ORIGIN      
        1 catatcaaat acacccatta aaaggaatac ccatgaaact cctcttcatc ctcctagtcc
       61 tcttcgtcgc cgtcgaacat ttctacatcg cctggcttga aatgacacag attcccagcg
      121 aaaaagcggc ggaaacgttc aagctgcctt atgaatttat ggaacaaaag cgcgtgcaga
      181 ccctgttcag taaccaaggg ctgtacaacg gctttctcgg catcgggctg gtgtggtcgc
      241 ggtttgccgc gccggataac gcggtgtacg gcgcgacggt actgtttctc ggcttcgtcc
      301 tgattgccgc cgcgtggggc gcgttctctt ccggcaacaa aggcatactc gtcaaacaag
      361 gtttgcccgc atttttggca gcggcggcgg tgttggcggt atgaaaaaaa tcaatgttgc
      421 ccccgaaaat ccccaatacc gcatcgtcga aatctttgaa agcctgcaag gcgaaggctg
      481 gaacacgggc atacccgccg ttttcgtccg cttgggcaaa tgcaacctcg cctgcggctg
      541 gtgtgatacc gattatttga cattcggcat gatgagcctg tccgacatct taggctgtct
      601 gaaaacctat gccgcccgca acatcatcat caccggcggc gaaccgacca tccagccgca
      661 tctcgatatg ctgctggaca tgctcaaggc ggaaggttat ttcctctgta tcgaaaccaa
      721 cggactcaat cccgcgccgc cgcaaatcga ctatgtcgcc accagcccca aagcctgcta
      781 tgccgccaaa tacgaaaaaa gctgtatcga aacagccgac gaagtgcgga ttgttgccga
      841 cggcgatgtc gttgcgttct gtgaaaatat ggaacgcaaa atccgtgcgc atcattacta
      901 cctttcgccc tgtgagcaag acggtgcgat gaacatctac gacaccatcc gccaaatcgg
      961 tattctgaac agtcgccccg acgcgccggt gcattggcag ttgagcgtgc agacgcacaa
     1021 atgggcggga atagagtagt ttaagcagtg taactcaaag ggacggcgta cggttttacc
     1081 gatgtttgac atacggggaa agtgtgtcgc ttctgcgtgg aaatgccggc atttccaccg
     1141 cccaatcagg acggagcctt actgaataag atgctgccgt tgggtacaag ctcggcttcc
     1201 taaattccga tggtcttttg aaccttgccg ataccctttg tcagtgcgcg caaatggcag
     1261 ggttagggaa aacgaaatgc cgtctgaaac agcattctgt ttcagacggc atttttctgt
     1321 tgacgtcaaa aggaaaaact gcctcggcaa tggataccga ggcggtttgg atttggtcgg
     1381 aatgagagga ttcgaacctc cgaccccttc gtcccgaacg aagtgcgcta ccgggctgcg
     1441 ctacattccg aattaagtaa ggcgtgatta tagcgcaaaa aatacggcgt gcctataccg
     1501 ttttgccttt ttgccgcgtg tcgggcggat ttaaaaggct tcgccgactt ttacgccgcc
     1561 cgccgtgtct tgcggcgagg caaggccggc aacaaaggct tgcgccgctt ggaaatccgc
     1621 cgtctgtata acggcttgcg cggcggcact gccgagcgtg ttggccatat attgccaacg
     1681 ttgcgccaaa gcgggattgt caggaatgcg gaaatcatcg cgcagttcgt ccacaaggtc
     1741 gggacggttg cagacgagga cgatgtcgca acctgcctca aaggaaatgc gggcgcgttc
     1801 tttgatgccg cctgccccgc acgcgccctc catagttaaa tcgtccgaga aaatcacgcc
     1861 tttgaacccg atgtcgcggc gcaaaatttg tttgagccag atttcggaaa acccggcggg
     1921 ctttgtgtcc acttgcggat aaacgacgtg ggcgggcata accgctgcca taccttcgcg
     1981 gctcataatg tggaaggggg cgaggtcggc ggcttcgagt tcggacaggc tgcgcccgtc
     2041 ttcgggtagc accaaatggc tgtctccttc gacaaatccg tgtccgggaa aatgtttgcc
     2101 gcaggatttc ataccgcctt tttccaaacc tttttgaagg gcgagggcaa ggtgggcgac
     2161 cgcttcggga ttgtggtgga aactgcggtt gccgatgacg gggcagtttc cccagtccaa
     2221 atctaagact ggcgtgaagg acaaatcgat gccgcaggcg gaaagctcgg ttgccaacac
     2281 ccggccgact tgtccggcgg cggtttcggc ggcggacgcg ccgtctttgt cccaaatttg
     2341 ccctaaaacg ttcattgcgg gcaggcgggt gaagccttcg atgaaacgtt gcaccctgcc
     2401 gccttcgtga tcgacggcga taatgagttc gggtgtgcgc agggctttga tttcggcggt
     2461 gagttctttg agctgtgcga tattttggaa gttgcggcgg aagaggatga tgccgcctac
     2521 ggcgggatcg agcaggcgtt gcttttcctc ttcggtcagg cggaaggcgg caatgtctgc
     2581 catgacgggg ccgcgcggaa tatggggaac agtcatttcg gtttgctcca aaaagcttca
     2641 gacggcatat gccgtctgaa cagggaaagg ggtcaggcgt tggcgcgttt tttatctttc
     2701 aacagaaaaa tcagcaccgc caatacaatg cctgtcgtgc caaagcccaa cagcgcggat
     2761 tttgtcagac ccaatgcgag gtagcccgat gcggcggcgg cggcaacggt taaggcgtaa
     2821 ggcagttgcg aggtaacgtg gtcgatgtgg ttgcagcgcg ctccggtgga cgacaggatg
     2881 gtcgtgtcgg aaatgggcga gcagtggtcg ccgcataccg cccccgccat tactgcggac
     2941 atacacggga taatcagcgc gggttcgact ttgaccgcca tggcggcggc aatcggcagc
     3001 ataatgccga acgtccccca gcttgtgcct gtggcaaacg ccatcacgct ggcgagcagg
     3061 aagaggatga cgggcaggaa gccgggatgg atgttgcccg caaccagtgt ggagaggtaa
     3121 tcgccggtgt gcatttcgcc gacaaccgta ctgatgagcc aagcgaggat taaaatggcg
     3181 attgcgccga acatagattt cgcaccctgc caaacggctt tgggatagtc ggcggtttta
     3241 atcgtgccga gcgtgcagag aacgacggca aggacgccgc aagtgccgcc gaataccagc
     3301 gaagtgttta cgtccgtgtt ttcaaatgcc cccaaaatgc tgaaggtttc gcttgcctgc
     3361 gcgccggtgt agatcatggc ggaaaccgtt gaggcgatta aggccaaaac gggaataatc
     3421 agtgcgtaaa cacgaccttt ggtagcgtct gaaacggcag tttcatcgtg ggcttcgttc
     3481 aacgcggctt gttcgaaacg tgccatcgag ccgatgtcga aggaaaacca tgcgacgacg
     3541 aacaccataa tcagggcaaa cagtgcgtaa tagttcatca ggctcatggc gacaaacgtc
     3601 cccatcggcg tgtattcggt gattttgtag gtaacgagca gtccggcaag cgtggcgata
     3661 atcgacgcgc cccagcttga aacgggcatc agcacgcaca taggcgcggc agtggagtcg
     3721 aggatgtagg cgagtttggt gcgggaaact ttaaacttgt cggtaacggg gcgggcaatc
     3781 gcaccgacgg cgagactgtg gaaatagtcg tcgataaagg ttacgaacac gaggcaggcg
     3841 gtcagcattt tcgcgccgcg ccggttttta atgtgccgtt ttgcccagtc ggcaaacgcc
     3901 tgattgctgc cggagtaggt cagcagggaa gtaaaaatac ccaaaagtat caggaaaacc
     3961 aagatttttg gtttgcccag cgaccaatcg ccgtctgacc aagccaagcc gacgaccatg
     4021 tctttcaggt gtgtcagacc gtcgacgggg ttgccgccga ccaaaaaggc aacgccgacc
     4081 agaataccga tgcctaaaga cagcagtacg cggcgggtaa tgatggcaag tgccagtgcc
     4141 aaaaagggtg gcacaaccga gaaaaatgaa tgtgaatagt cgatcagctg catggttatg
     4201 ggggtgttaa gcgtccggat ggggcggttt ctgtccgcaa cggttcggat tttgttggca
     4261 aaatgggcga aagtattttc tgttccggaa aatacttatt tgaaatcaat taactgattt
     4321 tttaaaatgc ccgttaatcg gtattgacgg gcattttatc atttaaaaaa tattttggtt
     4381 aaattatgtg ggttattgca ggtttaatgc gataaacagc gtgttgccac ggcgcatgac
     4441 cagcaggggg acgtttttgc ctgccttgtc catagctttg cggaaactgg cttcgtcatt
     4501 gacggggact tgcccgacgg cgaggatttc gtcgccgcgc ctcaagcctg cgcgttctgc
     4561 cgcgtcggaa acccgtacga cgacgaggtg tccgccgctg ctgtcggtat gtgtctgaag
     4621 ggtaatgcct gcggattcga ccgagaacgt accggattgc tgttcggtgt agggggcttc
     4681 atctgttttg gatgatgcgc cgatatgctc ggcggcgttg cccagcttga ctttgattgt
     4741 gatttcttcg cctttgcgcc atacgccgag gctgacttct tttcccggcg taatggcgcc
     4801 gaccataacg ggaaggtcgc cggaagaacg tatttctccg ccgtcgaggc tgaggacgat
     4861 gtcgcccgcc tgcaggccgg cacgttctgc ggggctgccg ggcaggattt tggcaatcag
     4921 tgcgccgccg gctttgtcca aaccgaacga ttgtgccaaa ccgtaggata cttcttgaat
     4981 aatcacgccc agttgtccgc gttggacttt gccggtgttt ttcagctgtt cggcgacatt
     5041 catggcaacg tcaatcggga tggcgaagga aatgcccatg aatccgccgc tgcggctgta
     5101 tatttgcgag ttgatgccga cgacctgtcc ttttaagttg aacagcgggc cgccggagtt
     5161 gcccggattg atggcaacgt cggtttggat gaaaggtgtg tagctttcgt tgggcaggct
     5221 tctgcctttg gcggacacga tgccggcggt cacgctgttg tcgaagccga agggcgcgcc
     5281 gatggcggcg acccattcgc ccggtttcaa atctttggga ttgccgattt tgacgacggg
     5341 cagctcttcc gttgcatcga ttttcagaag ggcgacatcg gattggacat ccgagccgat
     5401 gagcttggcg gtatattcgc gcttgtcgtt gagcaggact ttgatactgc ccatgccggt
     5461 aacgacgtgc gtattggtca gaatatagcc gtctttgctg atgatgaagc ccgaaccgaa
     5521 gttcaatccg ccgtcatctg cttcttcttg ggggatttcg ggcatgttcg ggacgaggcg
     5581 tttgaaaaat tcgtagaacg ggtcgctgtc ggcaagcggg tcggaatcgg tttcggcatt
     5641 gccgctgccg ttttgggtgc gcggggcggg ggctgcctga atattgacga ctgccgggcc
     5701 ttcgctttga accagttgga caaagtcggg cagcagcata ctgacgctgc cgtcgtcttt
     5761 ggtgtgttcg atgcgttcta cgaaggatgc ttcttttttg tccgcaccga aaaagctgcc
     5821 ggctttgtcg cagcctgcca gcgaggcggc acacagtgct gccaaagcga ggtattggta
     5881 ttttttgaac acgttttgtc ctttgtcgga tgccggtacc ggctttaatg ccgtctgaag
     5941 cgcattttgt cggcttcaga cggcataggt tgaaattcta caacgtccgt ccgaattttc
     6001 aagcgtttca ttttgaaggg cggcggcggt caggctttgg cgggatattc gcacaaatcg
     6061 ttgatgatgc aggtttggca ttgcggtttg agcgccttgc aggtgtagcg tccgtgcaaa
     6121 atcagccagt ggtgcgcgtc catcagaaat tctttgggga tgaagcgcat cagtttgtct
     6181 tcgacttcgc gcacgtcttt ccccggcgcg attttggttc ggttggacac tcggaaaata
     6241 tgcgtatcga ctgccataac gggatgtccg aacgccgtgt tcaataccac gtttgccgtt
     6301 ttgcgcccca cgcccggcaa ggattccaaa gcctcgcggt cttccggcac ttcgcccttg
     6361 tatttttcca gcaggatgcg gcaggtctgc ataatgtgtt tggatttggt tttatacaac
     6421 ccgatggttt tcgtgtattc catcacgccg tccaaaccca aatccagcat cgcttgcggc
     6481 gtatcggcaa tcggaaacag cttcgccgtc gccttgttta cgccgacatc ggtcgcctgc
     6541 gccgaaagca gaacggcgat taaaagctcg aaaggggagt tgaaattcag ctcggtggtc
     6601 ggatgggggt tggcggcgcg gaagcgttcg aaaatttctt ggcggatgtg tctgttcatt
     6661 tttttgtgca gtcggtttgt gcggtcggca ttataacgca cggttcaggc ggcgtaatat
     6721 tgcattcccc acagaatgaa ggcgtaacgc gccgttttgc cgataaccag catcagcccg
     6781 cttgtccacg gattcaaccg cagccagccg gcggcaagcg gcagtgcgtc gccgacgacg
     6841 ggcagccagg caaacggaag cagccaaata ccgaaacgcc gcatcagatt cagtgttttt
     6901 tcagacggca tttttcggga aggcagcaaa cgtcccatcc aataggaaac catactgccc
     6961 aatccgttgg caaggccggc gcacagcaac gcgccgtatg cgtgttcggg aaagcggcgg
     7021 acgaacaggg caaaggcggc ttcggatgtg ccgggcagga gggtggcgga agtgaaggcg
     7081 gaaaaggcga gggcggcgta ggtgtaggag ggtatcattg caaacagtct caaacaggta
     7141 acaatcggcg acggattgta cg
//
LOCUS       DAWFJW010000098         7056 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFJW010000098 DAWFJW010000000
VERSION     DAWFJW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.098"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(86..478)
                     /gene="rpsI"
                     /locus_tag="WGR19_001663"
     CDS             complement(86..478)
                     /gene="rpsI"
                     /locus_tag="WGR19_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0799156.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(488..919)
                     /gene="rplM"
                     /locus_tag="WGR19_001664"
     CDS             complement(488..919)
                     /gene="rplM"
                     /locus_tag="WGR19_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0799157.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1097..1276)
                     /gene="ssrS"
                     /locus_tag="WGR19_001665"
     ncRNA           complement(1097..1276)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGR19_001665"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1282..1587)
                     /locus_tag="WGR19_001666"
     CDS             complement(1282..1587)
                     /locus_tag="WGR19_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0799158.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1598..1927)
                     /locus_tag="WGR19_001667"
     CDS             complement(1598..1927)
                     /locus_tag="WGR19_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799159.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(1982..2971)
                     /locus_tag="WGR19_001668"
     CDS             complement(1982..2971)
                     /locus_tag="WGR19_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0799160.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3094..5796)
                     /gene="ppc"
                     /locus_tag="WGR19_001669"
     CDS             complement(3094..5796)
                     /gene="ppc"
                     /locus_tag="WGR19_001669"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0799161.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5839..6732
                     /locus_tag="WGR19_001670"
     CDS             5839..6732
                     /locus_tag="WGR19_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0799162.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKLKTEALADRLRHINHTVDVRTINEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQAKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
BASE COUNT         1387 a         1818 c         2052 g         1799 t
ORIGIN      
        1 tctgtaatct ttagattgcc aatgggaaac cgtctactac aaataaaaaa ccctgcgata
       61 agcagggttt tttgaatttc caacattaac gtttggagaa ttgttttgca cggcgtgctt
      121 tgcgcagacc cggtttttta cgttcgactt cgcgggcatc gcgggtaaca aaaccagctt
      181 gagacaaggc gggtttcaac gcggcatcga agtcgatcag ggcacgggta atgccgtggc
      241 ggattgcgcc ggactggccg gtttcgccgc cgccaacaac attgactttg atgtcgaaag
      301 attcggcgtt ttcagtcaga accaagggtt ggcgaacaac cattcggctg gtttcgcgtg
      361 cgaagaattc gtcaacggga cggccgttta cgatgatttg acctgtacct ttaatcagga
      421 atacacgagc cactgaactt ttgcggcggc ctgtgccgta gtagtattta ccgttcatgt
      481 cgcgtcctta tttcagttcc aaaactttgg gttgttgcgc agcatgggcg tgttccgcac
      541 ccgcatacac tttcagtttt ttaatcatgg cgtaacccag aggacctttg ggcagcatac
      601 cttttacagc ttgttccaaa gcgcggcccg ggaattgctc ttgcatttcg cggaaagtgc
      661 gttcgtagat accgcctggg aaaccggaat ggcggaagta ttttttatct tcgaatttgg
      721 caccggttac acgcagtttg tccgcattga taacaatgat gtaatcgccg gtatcgacgt
      781 ggggggtgta ttcaggtttg tgtttgccac gcagacggct ggcgacttcg gccgcaacgc
      841 gacccaagac tttgtcttgg gcatcgatga cgaaccattc gcgcttcacc tcgtggggtt
      901 tcgctgaaaa ggttttcata gtggaaatcc agatagatat agaaagttgt aaattttaaa
      961 gacaggattc gattttgtca atcgcattac cgcgttacgg aaggattttc cggatttcgg
     1021 cagactgcac actgcttttt cgggcggggc ggcggccaat gtgaaaaacc gcatcgttgc
     1081 gatgcggttt tgaatgggaa tccccgcgag agccgtttcg gccgaatccg cttgaacctt
     1141 gctgacaagg cggctgcctc gggtagtttc gggtgcgtcc gcaaaaggac gctcgcgccc
     1201 actactgctc ccggcaacct taagcgaact tattggttca aaggaatata tgccttcgcg
     1261 gacaccgcag ggaaaaaggg gttattcctg cgccaagcgg gatagtgctt tttggcaggc
     1321 gttgtccata tcggctattt tacgcgcaaa atcgccgatt gccaaatcgc cgccgttcag
     1381 ggaggttttc aacaggtcgt ggacgacgtt gagcgcggcc ataatgacga ttttttcgct
     1441 gtccgcgacg cgtccgcctt cgcggatggc ttcggctttg ccgttgagca ttccgactgc
     1501 ctgcaacagt gtgtcttttt cttctgccgg cgtgttgacg gtcagccggg cgtgcatgac
     1561 ttcgatgtgg acttgttcga tgttcatcct ttaatcctta ttgctgcgtt tcctgccgtt
     1621 gggggaggcg cgctgccagt gcgctgattt tttccctgct ctgttcgagc aggctgcggt
     1681 atcgtgtatt ttcttctgtc aggctgtcga ttttgttttg caggtcttct ttgagtttgc
     1741 cgacttggac gagcagggct tcgctgagtt cgtcgacggc ggtttcgtgt tcgagttttt
     1801 gccgctcgtg cgcccgtttg agttcggcga cggtttcttt gaggcggcgg ttttcgctga
     1861 cgagggtttc gaatttttgt accagcgtat aaacgctgct ttcgagtttt tcgatatttt
     1921 gtttcataac cttacctgtc cgtatgccgt ctgaaggcct cagacggcat ctgtctgttg
     1981 tttattcgaa acgcgcgctg cgttccatca gtctttcgac gacctgctga ggggtcattt
     2041 ccttacggat gagttgcagc agggtttggg taatcggcat atcgatttgg tacttacagg
     2101 cagtattgaa gacttcttct atcgtgctga ccccttcgga aacgtgtccg atttcgacca
     2161 gcacctgatg cagttccttg ccttctgcca aacccaagcc gacgcggcgg ttgcgtgaga
     2221 gtgcgccggt gcaggtcaag atgaggtcgc cgatgcctgc cagccccatc atggttttgg
     2281 gctgtgcgcc cattgcggag gcaaggcggg taatttcagc taatccgcgc gtaaccagtg
     2341 cggcacgggc gttaagcccg tactctaggc catcggacaa tccggtggca atcgccataa
     2401 cattttttac cgcgccgcca accgccacgc cgataacatc ggtactgccg taaagcctca
     2461 tgacggtcgt gttgagctgc ggtacgagtt cttcaatcca ctcttggttt tcggaggcga
     2521 ggacgacggc acagggcagt tgtttggcga gttcctgtgc aaaactcggg ccggagagta
     2581 cgccgatttt cttattgtcg ggcaatactt ctttcaatac ttgaaaggtc agcagccctg
     2641 tatcctgctc gaatcctttg caggcggcga ggacggggag gtgtcccgcg ccgtactgtt
     2701 tgagcagctc tgcgctgctt ctcaatccgg caacggaggt tacgataagg acaagtccgc
     2761 tgtctttgag cgcgtctgcc aaatccgcac acacttccaa ggtttcggga aaggaaaagc
     2821 cgggcagtcc gcgtttgttt tcacgcgctt cctgcatttg gcggacttgg tctgcgttgc
     2881 gcgtccacag ggatacgcgg ttgccgtgtt gggaaaagtg cagggcgagc gccgtacccc
     2941 acgaacctgc gccgataacg gtaattttca ttggtcgtct ttcaacatat cactgccgtt
     3001 cactttaaaa caatcggtgt ttctctgcaa gtgcggtcag ggaaatgccg tctgaaaggc
     3061 gttcagacgg cattttgccc cgatgcggca ctatcagcct gtattgcgca aaccttgcgc
     3121 cacgccgttg atggtcaggt gaaccatcag cagcgcgtgc ggattgtcgg gttctttacg
     3181 caggcgtttg agcatggcga cctgcaggcc gttgagggcg ttcaaatatg gaatcctcaa
     3241 ggcaagcgaa cgggcgaggc tgcggttgtc gcgtaaaagt tcttcggttt gcagcaggtc
     3301 gagcagggct ttgcggctgc gttggtattc ttccttaatc atgccgaaga tgacggcggc
     3361 tttttccggc gattcgctca agccggcata gttttccgcg agggtgatgt cggttttcgc
     3421 catcacttgc tccatattgg aaagcatggc ttggaagaac gggttgcttt gggcgtgttc
     3481 gcgcagggcg gcgagcgttt cgggtttgtc ttcgcacaag gtttccaccg cgctgccgaa
     3541 accgtaccaa gcaggcagca tgaggcggtt ttgcatccat gagaataccc acggaatagc
     3601 gcgcaagtcc tgaatccgcg ccaaggtttt gcggctggca ggacggctgc cgaggttgag
     3661 ggtggcgatt tcctgaatcg ggctggtttg caggaagtag tcgatgaagt cggggtgggt
     3721 aatcagttcg cggtagtatt tgaacgatac gtccgacaat gcctgcatca gtttggcatc
     3781 agggtctttt ttatccggca ggatgctggc ttccaaagtc gcggcaacca aggtttccaa
     3841 gttgcgttgg gcattgccgg ggtcggcgta tttggcggta atgacttcgc cttgttcggt
     3901 aatgcggatt tgtcccgcca cgctgcccgc cggttgggcg agaatggctt ggtaagaagg
     3961 gccgccgccg cgtccgacgc tgccgccgcg accgtggaac aggcgcatgc ggacatcgta
     4021 ttttttgaag agttcgacca agcccaattc cgcctgatac agacaccatg agctggtaac
     4081 atagccgccg tctttgttgg agtcggagta gccgagcata atttcttgga tgttgccgca
     4141 gctttcgagc agggcatcgt accaatcgag gcggaacatg gtttccatga ccggacaggc
     4201 gttttcgagt gcttcgatgg tttcaaacag cggcacgata ttgatgcggc tgcgcggttt
     4261 gccgttttcc accgccagca ggccgctttc tttcagcagc aatgccaagg cgagcaggtc
     4321 gctgggttgt tcgcagttgg aaataatgct ttgtgtaacg gcatcttcgc cgaattcgtc
     4381 tttgattttg cgcgcttcgt tgaaaattgc cagttcgtgg cgggtatggt cgctgtatgt
     4441 gatgaacggg ctgtacagcg gacgttgatg gctcaattcg cgcaacaggg cggcttgttt
     4501 ttgctcttcg ttcaggctgt tgtagtcttc caagcctgcg tgttggaaaa gctcggcaac
     4561 cacatcggcg tgtttgcctg cgtgttggcg caggtcgagc ggcatcatgt ggaagccgaa
     4621 cacggacacg gaacggatga ggtctgccaa acggccttca gcaagcaggc ggctgccgtt
     4681 gtcgataagg gaacgttgca attttttcaa atcatcgaga aattcttgtg ccgaagcata
     4741 cggctcgaga aagccgaatt tgcagcccat gcccaaaccg agcgcgcgcg ctttgcccat
     4801 agcgcgcgcc atgatgtaag caatggcgcg gcggtagggt tcttcggtgc gagcgatttc
     4861 ttcgtcaggc gagagggctg ccagtgccat tacgtcgtcg ttgactttga cgcggcggat
     4921 ggagagcggc agttcgcggt aaagtttgtc gagttcgcca cggtaaaaac ggaacacggc
     4981 atcggcgtgg cggcggaagg caaaacgcaa ggtttcggca gaaacgaacg gattgccgtc
     5041 gcggtcgcca ccgatccagc cgccgatttt gaggatgttc ggaacgcaga catcgggata
     5101 gaccatctga aagtcgtgtt ccatattgcg gtagagcttg ggcagggctt cgaaaaagct
     5161 catcgggaag atggacacgc cgttgttgat ttcgtcgttg acgctgagtt tgtggcggcg
     5221 cgtttcgctg gtctgccaca agcccagcag gatagtgtcg atttcgcggc gcagccgtgc
     5281 cagcgcgtcg gcattggtgc agcgttcgcg ttgcggcaac agtgcgcgga tgcggcggtt
     5341 gaagcttaag acggtttggc gttgcacttc ggtcgggtgc gcggtcaaaa cggcggtaac
     5401 ggacgtattg tccaactgcc gctgcaccga tttgccgtcg gctttccccg ctttgagcct
     5461 gcggacggtt tccgtcaggc tgccttccgc gccgccgcgt ccggcttctt cgtggatttg
     5521 gcggcggcgt tcgtggtgca cgtcttcggc gatattcaaa atctgggcga acaggccgca
     5581 ggccaaggtt aaatcgtggg tttgttgttc gtccaattgc ggcaatactt tttcaatcaa
     5641 tgccgcgctg tcgtcggaag tggacaagag tttgaccgtt tcgacaacca atggcgaggc
     5701 ttcttcgtgc aggaggttga acagggattg tttcagaaat tccgcgtccg ccgccaaagc
     5761 cgcgtccttt ggattgttca gaatatgcag ttgcatgatt tttctctcat tgccgtaaat
     5821 actgtaaatg tacctcaaat gccgcatccg tgccaaaccg ttcacacttt aaccgcccgt
     5881 gtcccgaaat gccgtctgaa gttgaacgcc gcccgacggc agcgttacaa tcgcccacaa
     5941 ctgttttttc cgaacatcat catgaccacg accgaacacg acaacgacga tgcattcctg
     6001 ctgcggtaca gccgccacat cctcttggac gaaattggca tcgaagggca gcagaaactt
     6061 tccgccgcgc atattttggt cgtcggctgc ggcggtttgg gtgccgccgc cctgccctat
     6121 ctcgccgctt ccggcatcgg cacgctgacc atagccgatt ccgacacggt cgagctgcac
     6181 aacctgcaac gccaagtcgc atttgacgag ggcgatgtcg gcaaactcaa aaccgaagcc
     6241 ttggcagacc gtctgcgcca tatcaaccat actgtcgatg tccgcaccat caacgaaaaa
     6301 cttgacggct gccgcctgac cggtttggtt caagccgccg acatcgtttt agactgctgc
     6361 gacaactacg ccacgcggca ggcggtcaac cgtgcctgcg tgcaggcgaa aacaccgctg
     6421 gtttcagggg cggcggtacg ctttgaaggg cagcttgccg tgtaccgtcc cgacttgccc
     6481 gactcgccgt gttacgcctg cctgtttgac ggcggatcgg cttcagacgg catctgttcc
     6541 ctcttcggcg tattctcgcc gctggtcggc atcatcggca gtacccaagc ggcggaggct
     6601 ctgaaaatcc tgctggatgc gggcgaaccg tcgcacggca ggctggcggt ttaccgtgcc
     6661 ttggaagggg gctggcaata tttcgacctg ccgcgcaacc ccgaatgccc gatttgcggc
     6721 gcggaacggt aaaaccctgc cgccgtttca gacggtatcc aaacggatgc ggcggaacgg
     6781 ttttaaaaat ttaaaaattt acatttcttt gcaaaaaaaa ataaacttac cttataattg
     6841 caattgtttt agcaatatct gattcgcaga ttcattgagc taagatattt tccccgtaat
     6901 gtgtttggcc gtctgtccct tttgggttcg gacggctttt tttaggctgt atttgaatac
     6961 ccgattgatt tcgcctgttt gtagggggaa gccgcttatt tccgttcggg tagaaacgct
     7021 cccatcggat aaaaagcatt ttgtccgact gattaa
//
LOCUS       DAWFJW010000099         7007 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFJW010000099 DAWFJW010000000
VERSION     DAWFJW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.099"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            216..1400
                     /gene="ubiM"
                     /locus_tag="WGR19_001671"
     CDS             216..1400
                     /gene="ubiM"
                     /locus_tag="WGR19_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0799163.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1671..1826)
                     /gene="rpmG"
                     /locus_tag="WGR19_001672"
     CDS             complement(1671..1826)
                     /gene="rpmG"
                     /locus_tag="WGR19_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0799164.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1858..2091)
                     /gene="rpmB"
                     /locus_tag="WGR19_001673"
     CDS             complement(1858..2091)
                     /gene="rpmB"
                     /locus_tag="WGR19_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0799165.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2341..3867)
                     /gene="farB"
                     /locus_tag="WGR19_001674"
     CDS             complement(2341..3867)
                     /gene="farB"
                     /locus_tag="WGR19_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0799166.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVFFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPIFGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3891..5045)
                     /gene="farA"
                     /locus_tag="WGR19_001675"
     CDS             complement(3891..5045)
                     /gene="farA"
                     /locus_tag="WGR19_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0799167.1"
                     /translation="MDTHTDETKLQNTQAKRKRCLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      5438..5482
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5488..5961
                     /gene="queF"
                     /locus_tag="WGR19_001676"
     CDS             5488..5961
                     /gene="queF"
                     /locus_tag="WGR19_001676"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0799168.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPIGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            6035..6721
                     /locus_tag="WGR19_001677"
     CDS             6035..6721
                     /locus_tag="WGR19_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0799169.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHDGGWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWVAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1740 a         1991 c         1647 g         1629 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatacat accccacacc cgatttgata cacattaagc
       61 tgaaagtaaa aatccgcata caccctcccc tgcatatttc ttcaacagtg ggttttgata
      121 taatcgccta tctgttacag atagttcaaa cggcattcaa acccttacaa ataccgtctg
      181 aaatcatcgg catccgtcta tgcaaaggat attttatgag tttacacagc gacatcctcg
      241 tcgtcggcgc aggtccggcg ggtttaagtt ttgcagccga acttgccggc agcggtttga
      301 aagttaccct gatcgaacgc agcccactga ctgttctgca aaatcctcca tatgacggac
      361 gcgaaatcgc cctgacccat ttttcccgcg aaatcatgca gcgtctgggt atgtgggaca
      421 aaattcccga aaatgaaatt taccctttgc gcgatgccaa agtgctgaac ggacgttccg
      481 actaccagct ccactttccc caaccgaccg aggcgcgcgg cgaacctgcc gactgtttgg
      541 gctatctgat ttccaaccac aatatccgcc gcgctgccta cgaagtcgta tcccaactcg
      601 acaacattac cattctgacc gatacggccg ttaaagaagt caaaacatct gacaacgaag
      661 cacaagtcat tttagaaaac ggcaaaatcc tgaccgcccg cctgcttttg gcggcagaca
      721 gccgtttttc gcaaacccgc cgacaactcg gcatttcttc agatatgcac gactacagcc
      781 gaaccatgtt cgtctgccgt atgaaacata ccctttccaa ccaacatacc gcttacgaat
      841 gcttccatta cggacgcacc atcgcactgc tgccgctgga agagcacctg accaataccg
      901 tcattaccgt cgataccgat aaaatcaaca gcgttcaaaa cctttcgccc gaagaattgg
      961 cggcaagcgt aaaagaacaa ctcaagggta ggcttggcga catggaactg gtcagcagca
     1021 tccaccatta cccccttgtc ggtatgattg ccaaacgttt ctacggcaaa cgcagcgcgc
     1081 tgatcggcga tgcggctgtc ggtatgcacc ccgttaccgc acacggtttc aacttgggtc
     1141 tgtccagcgc agatattttg gcaaaactga tactcgaagc cgagcagcgc ggtcaggaca
     1201 tcggcgcggc ttccttgttg gaaaaataca gcagcaaaca tatgctgcac gcccatccgc
     1261 tttatcacgg gaccaatatg atgctgaaac tgtttaccaa cgaaaccgcg ccggcaaaac
     1321 tgctgcgcgg tttggtattg cgtgccggca acaacttccc gccgctgaaa aagctgatta
     1381 ccaaacaact gaccggttga aaactgccga aatgccgtct gaaacgtttc agacggcatt
     1441 tttgtattat agtggattaa caaaaatcag gacaaggcga cgaagctgca gacagtacaa
     1501 atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc tctatccttg
     1561 gcagacggtt tgcagcagat tcgcatacag cagatgtttc aagcggaaaa gaaacacgat
     1621 aaaaataaaa aagcctccga acagtcggag gctttgcttt caaactgaaa ttatttcagt
     1681 ttggtttctt tgtacactac gtgtttgcgg gcaaccggat cgaatttttt gatttccagt
     1741 ttgccgggca tagtgcgttt gtttttggta gtggtgtaga agtgaccagt acctgcactg
     1801 gattccagtt tgattttatc gcgcattgca gtaatcctta attaaatagt gtttaaatta
     1861 agcttcaccg cgagcacgca aatcagccaa tacgacatca atgcctactt tgtcgatggt
     1921 acgcaatgca gcgttggaaa cgcgcaggcg aacccagcgg ttttcacttt ctacccaaaa
     1981 acgacgtgat tgcaagttgg gcaaaaaacg gcgtttggtt ttgttgttgg cgtgcgatac
     2041 gttgttgccg gacatcgggc gtttgccggt cactttgcaa actcgtgcca taatcagtct
     2101 ccaaacttct aaaatagtaa aacgcgattt ataccacgaa aaacagtgtt agttcaagtg
     2161 ttttaaagaa aaacggctgt tttgcgcgga taatccttcc gattgtcgca tctgtggaac
     2221 gtttgcaggt gcgaaacaga tggggcgcga ttatagcgga tttcgggaac tgctgcattc
     2281 aaaatgccgt ccgaacatat ttccagtatt ttcagacggc atttcaagtt ttcaaatccc
     2341 tcaatgtcca ccgccgccgc cgttgtggaa cggcggtttt gccagccata tgacgggtat
     2401 catgataatg aataacaagc tgcccgccat aaagatttcg ttcgagccga taataaaacc
     2461 ctgctgggta atggtattgt tgatgatgcc tagggtttga atgtcggaaa cgccgtgctg
     2521 ggacaaatga gcggccgttt cgtgcaatgt tgcggaatag ggcgtgatgt gttcggcaaa
     2581 gcgtgtatgg tgcaacgctt cacgccgttc ccacagggta ctaacgacgg atacgccgac
     2641 accgcccatc agcacgcgca ggaaattcga caggctgccg gcggcggcaa tctgtccgcc
     2701 cttcatgtgc gacagtgtga tggtggtcag cggcaggaag aacatggcaa cgcctactcc
     2761 ctgccaaaac tgcggccaga tgacgttgcc gatatccatg tcggcataaa aatcggtgcg
     2821 ccaatagaaa gtgaaggcaa aagtcaggaa gctggcggtt acgagcaggc gcatatcgat
     2881 tttattgccg aacctgccga ttaacggaga caggaagacg ggcaggatgc cgacgggtgc
     2941 ggcggcaagc cccgcccacg tggaggtata gcccaggttg gtctgcaaca ctaacggcag
     3001 cagggtcagc gtccccatat acaccataaa acccaatgat gtggcaatga tgccgacggt
     3061 aaaattccga tctttaaaca gcgataaatc gacaatcgga tatttttctc ccaattccca
     3121 aacaataaaa tacgacaagc acaccagtgc ggttacgccc aaggtaatga tttctcccga
     3181 agcgaaccag tcgagttcct tacccctgtc cagcatcatc tgtaacgcgc cgataccgac
     3241 taccatcaat gtaagcccga catagtcggt cggcatttta acggtttccg tttcccgata
     3301 tttcaaatgt ttccatgtaa tccatgccga tatgataccg atagggatat taatgaagaa
     3361 aatccaaccc caatgccagt ttccggaaat ccagccgccg aatatcggcc cgagaacagg
     3421 ggcgacaacg acggtcattg cccacaatgc cagtgccagc gtccgttttg cgggcggata
     3481 ggatgccatt aacaggcttt gcgacagggg aatcagcggc ccggcgataa aaccctgcaa
     3541 gatgcggaaa aacaccaaag cctgaaggtt gggcgcgatg ccgcacagcc atgatgtgat
     3601 gacgaaaccg acagcggcgg cggtaaacag tttgacctcg ccgatacgtt ttgccaaaaa
     3661 gcccgttaac ggcacggaaa cggcgtttgc cacagcaaaa gaagtaatga cccacgtccc
     3721 ctgcgtggtt gccgcaccga ggttgccggc gatgacggga acggcgacat tggcgatagt
     3781 cgtatctaaa acttccataa atacggcaag ccccaaagac agcgtaaccc acgccaatgc
     3841 cgcaccctta agcggtggat aatccattgt gtcctccgtt tcagacggca tcagggcgcg
     3901 gattgcccga ggatttcatc gaccgtccga tcgacttccg accagtcggt actttccatt
     3961 tccggcaatg ccgcacccgg cgtttttgaa acaggcgcgc ctgcggcgga agtatccact
     4021 ttaaccgtca tcgacaaacc gatacgcaac ggatgcctgt ccacatcttc gcggttcagc
     4081 acgatacgga cggggacgcg ctgcaccact ttaatccagt tgcccgttgc gttttgcgcc
     4141 ggaatcagcg aaaacgcgct gcccgtacct gccgaaaacc ctgccaccct gccgcgataa
     4201 acaatttgtt tgccgtacaa atcggacacc agttcggcag gctgtccgat tttcatatgc
     4261 cgcaactgcg tctctttaaa attggcatcc acccacacat ccgacagcgg caccaccgcc
     4321 atcagcggcg cgcctgccgc cacctgctgc ccgacctgca ccgaacgctt cgccacctga
     4381 ccgtccgccg gcgcgcggat ttgcgtccgc tgaaggttca accacgcatc tttcaacctg
     4441 ccgattgccg tctgaaccgc cggctgttcg cgcaaagaaa cctgaccgcc caaagccgcg
     4501 cgtgccgaag attcttccgc caaagccgct ttgaccgccg cctgagcctg agacactgcc
     4561 gcacgggcat gtgccagctc ttcggcggac accgcgcccg attccgccaa agcagaccgg
     4621 cggcgcaaat catcctgcgc acgtgccaaa tccgcccggc gcaaggcaac ctgcgccccc
     4681 gcctgcgaag tggcggcatt ttgccggcgg ttttgccgca ccgcctgaat cagctcgttt
     4741 tttgcccgct cgtaagccag cacatcatta tcgtcgtcca ataccgccag cacgtcgcct
     4801 tttttcacgg catccgtatc gtcgtgcaaa accttccgca ccgtaccgcc cttttgcggc
     4861 gtaacctgaa ccacgcgtcc ggcaacataa gcgtcttccg tttcctcttc gtgctgccac
     4921 cataaaaaaa acgccgaccc ggcggctgcg gcggcaagcg cgaacagcag cgtcaatgcc
     4981 gtcaggcagc gtttgcgttt ggcttgcgtg ttttgaagtt ttgtttcgtc cgtgtgcgta
     5041 tccataagat tgggttccga atttcctgat ttcatataaa caatgttctt tcaataggag
     5101 gcagattcta tatccgcgca ataacgcaat caagtcagta ttttagatgc aatattcaaa
     5161 aaacaacgct tcaggcggca aaactgaaaa tatatttcgt ttatatagtg gattaacaaa
     5221 aatcaggaca aggcggcgag ccgcagacag tacggatagt acggaaccga ttcacttagt
     5281 gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt
     5341 tttgttaatc cactatatat gacacggaaa acgccgccgt ccaaaccatg ccgtctgaag
     5401 aaaactacac agataccgcc gcctgtatta caatcgccgc cccgtggttc gaaaacctcc
     5461 cacattaaaa aactaaggaa accccctatg tcccgcaata ccgaagagct gcaaggcatc
     5521 tcgcttttgg gtaatcagaa aacccaatat ccgatcggct acgcgcctga aattctcgaa
     5581 gcattcgaca acaaacatcc cgacaacgac tatttcgtca aattcgtctg cccagagttc
     5641 accagcctct gccccatgac cgggcagccc gacttcgcca ccatcgtcat ccgctacatt
     5701 ccgcacatca aaatggtgga aagcaaatcc ctgaaactct acctcttcag cttccgcaac
     5761 cacggcgatt ttcatgaaga ctgtgtcaac atcatcatga aagacctcat tgccctgatg
     5821 gatccgaaat acatcgaagt attcggcgag ttcacaccgc gcggcggcat cgccattcat
     5881 ccgtttgcca actacggcaa agcaggcacc gagtttgaag cattggcgcg taaacgcctg
     5941 tttgagcacg acgcacaata aaccatcctt cccactttca gacggcattc cttcgatacg
     6001 atatgccgtc tgaacgcctt tccgaaagat ttttatgtac gcatttaccg acgcacagca
     6061 acagaaggca ctcttctggc tggtgctttt ccatatcctc atcatcgccg ccagcaacta
     6121 tctggtgcag ttccccttcc aaattttcgg catccacacc acttggggcg cgttttcctt
     6181 tcccttcatc ttcctcgcca ccgacctgac cgtccgcatt ttcggttcgc acttggcacg
     6241 gcggattatc ttttgggtca tgttccccgc ccttttgctt tcctacgtct tttccgtttt
     6301 gttccacgac ggtggttgga caggcttggg cgcgctgtcc gaattcaaca cctttgtcgg
     6361 acgcatcgcg ctggcaagtt ttgccgccta cgcgctcgga caaatcctcg atattttcgt
     6421 attcgacaaa ttacgccgtc tgaaagcgtg gtgggttgcc ccgactgcat caaccgtcat
     6481 cggcaacgcc ttagatacgt tggtattttt cgccgttgcc ttctacgcaa gcagcgatgg
     6541 atttatggcg gcaaactggc agggcatcgc ttttgtcgat tacctgttca aacttaccgt
     6601 ctgcggtctg tttttcctgc ccgcctacgg cgtgattctg aatctgctga cgaaaaaact
     6661 gacgaccctg caaaccaaac aggcgcaaga ccgccccgcg ccctcgctgc aaaatccgta
     6721 acacagccct gccgggaatc gggacggttg ggttttcttt tatttgcaaa cactgtcgga
     6781 acggataaat ccggattccc acccacttcg tcattcccgc gaaagcggga atccggtacg
     6841 ttgggtttca gtcatttccg ataaactcct gccgcgttgt atttctagat tcccgctttc
     6901 gcgggaatga cggcggatgg gtttccgtgc cgtggtgtcc ggattcccgc ccgtacggga
     6961 atgacggcag tgcttccttg gcttagtttt attttaatcg actgtat
//
LOCUS       DAWFJW010000100         6979 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFJW010000100 DAWFJW010000000
VERSION     DAWFJW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.100"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(108..1454)
                     /gene="mnmE"
                     /locus_tag="WGR19_001678"
     CDS             complement(108..1454)
                     /gene="mnmE"
                     /locus_tag="WGR19_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0799170.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPSELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVVCGEITGEFTADDLLGVIFSRFCIGK"
     gene            1589..1744
                     /locus_tag="WGR19_001679"
     CDS             1589..1744
                     /locus_tag="WGR19_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799171.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1741..2172
                     /locus_tag="WGR19_001680"
     CDS             1741..2172
                     /locus_tag="WGR19_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0799172.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2306..6511
                     /gene="app"
                     /locus_tag="WGR19_001681"
     CDS             2306..6511
                     /gene="app"
                     /locus_tag="WGR19_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0799173.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYQKDTKHYYHEDYHN
                     PRLNKFVTDAAPVEMTINMNGNTYGNITDYPDRVRIGSGRQYWHKKNGNPQQIFGSYS
                     YRIAGNTHNQVGGSDGHVSLNGDVKKPNNYGPLPTGVSFGDSGSPMFIYDAKKQKWLI
                     NGVLREGNPYQGTHNGYPIVRKAYLTKIQDGDTGASIYDMVGGASYLWEAKELGKSTI
                     NRKHITFGEYVEGGVGKLTLKSNIDQGAGGLYFEGDFIVSPTQNETWKGAGISISDES
                     TIIWKVNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSE
                     IGLVSGRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQ
                     DKESTVTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDR
                     TLLLSGGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIN
                     RTFKAENFHIQGGQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLT
                     NCVEKTITDDKVIASLTKTDISGNVSLADHAHLNFTGLATLNGNLSANGDTRYTVSHN
                     ATQNGDLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSH
                     SALNGNVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNAT
                     ITLNSAYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGT
                     FRFMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLN
                     FTLQNEHVDAGAWRYQLIRKGGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDA
                     LIAAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPA
                     TTAFPRARRARRDLPQPQPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRV
                     FAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTE
                     NTFDDGIGNSARLAHGAVFGQYGIDRFDIGISAGAGFSSGNLSDGIGGKIRRRVLHYG
                     IQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFK
                     PAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLH
                     AAAAKGPQLEAQHSAGIKLGYRW"
     gene            complement(6620..>6979)
                     /locus_tag="WGR19_001682"
     CDS             complement(6620..>6979)
                     /locus_tag="WGR19_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799174.1"
                     /translation="NPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVFWDGNK
                     RTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNCISGID
                     YFGTDEDIDFPDPPNMG"
BASE COUNT         1878 a         1970 c         1695 g         1436 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactataaa accaaacgga tcggattccc gtttttatgg
       61 gataacggaa tgttcagccg gacaccgcca cgatgctttc gatccgttta ttttccgata
      121 caaaacctcg aaaaaatcac gccgagcagg tcgtccgccg taaactcgcc cgtgatttcg
      181 ccgcacacga cctgcgccag ccgcaagtgt tcggcaaaca gctcgatttg atggttgccg
      241 cacaatgccg ccagcgacaa ttcttcctgc gctgctttga gtgcgttgac gtgccgcgtc
      301 cgcgccaaaa acagcccttc gctttcgccc tgccaaccgg cctcacgcaa caacgtccgt
      361 ttcagcgcgt ccaagccgtc gcccgttttc gccgacaacg cgatgacggt ttccgcgccc
      421 gtaccgaacc cgcctgccgc gtgtgcgtgc aaatcggatt tgctgtggat ttctatgcgt
      481 ttcaactccg acggcagcgc gtccaaaatc gcccgtgtct tgtcgttcac gccttcgcgc
      541 ggatcgacca acaccagcgc gacatcggct tcggatacgg ctttgcggct gcgttcgatg
      601 ccgatgcgtt cgaccacgtc gtccgtctcg cgcaaacctg ccgtatcgac aatatgcacc
      661 ggcacgccgt caatcaggat acgttctctg accgcatcgc gcgtcgttcc ggcaatatcg
      721 gtaacaatcg ccacttcgtc gcccgccagc gcgttcagca ggctcgactt gcccacattc
      781 ggcgcgccga ccaatacgac attcagacct tcgcgcaaaa tcgcgccctg ctgcgcgttg
      841 gcaagcacat catccacggc gcggcgcaag ccgtccagtt tgccgcgtgc gtctgccgct
      901 tcgagaaaat caatgtcttc ctcgggaaaa tctaacgtcg cttcgaccag catccgcaag
      961 gtaatcaaat cttcgaccag accgtgtatc cgccgcgaaa aatcgccctt gagcgaacgc
     1021 aatgccaaac gtgccgccga acggctggat gcgtcaatca aatccgccac gccttccgcc
     1081 tgtgccaagt ccagtttgtc gttcaaaaac gcacgtttgg taaactcgcc tggttcggca
     1141 aggcgcgcac ccaattccaa acagcggttc agcagcatat ccatcactac cggcccgccg
     1201 tgtccctgaa gctcgatgac atcttcaccc gtaaaacttg ccggtgcggc aaaaaacagt
     1261 agcagcccgc tatcgattgc ctgtccgtcc gcgtccgtaa aatcagcata ggttgccacg
     1321 cgcggcttgg gcgttttccc gcacaaagcc tgcgccatcg gcagcaggtt tttccccgat
     1381 atgcgtatca cgcccacgcc gccgcgccct ggtgcggtag cgactgccgc aatcgttgga
     1441 acgttatccg acataaaacc cccgaaaaac aatcaaaaac agccgtgatt atagcaaatg
     1501 ccgtctgaag tccgacggtt tggctttcag acggcataaa accgcaaaaa tgcttgataa
     1561 atccgtccgc ctgacctaat ataaccatat ggaaaaacga aacacatacg ccctcctgct
     1621 cggtataggc tcgctgctgg gcctgttcca tcccgcaaaa accgccatcc gccccaatcc
     1681 cgccgacgat ctcaaaaaca tcggcggcga ttttcaacgc gccatagaga aagcgcgcaa
     1741 atgaccgaaa acgcacagga caaggcgcgg caggctgtcg aaaccgtcgt caaatccccg
     1801 gagcttgtcg agcaaatcct gtccgacgag tacgtgcaaa taatgatagc tcggcgtttc
     1861 cattcgggac cgttgccgcc gccgtccgac ttggcgcaat acaacgacat tatcagcaac
     1921 ggggcagacc gcattatggc aatggcggaa aaagaacaag ccgtccggca cgaaaccata
     1981 cggcaagacc aaaccttcaa caggcgcggg caactgtacg gcttcatcag cgtcatcctg
     2041 atactgcttt ttgccgtctt cctcgtatgg agcggctacc ccgcaaccgc cgcctccctt
     2101 gccggcggca cagtggttgc cttggcgggt gctttcgtga ttggaagaag ccgagaccaa
     2161 ggcaaaaatt aatgacaaat cctagggcgt gcttcatatc cgcccgaacg ccgaaccgca
     2221 catataggca catcccgcgc gccgcccgga agcggaagcc gcgccctccc aaacaaaccc
     2281 gaatcccgtc agataaggaa aaataatgaa aacaaccgac aaacggacaa ccgaaacaca
     2341 ccgcaaagcc ccgaaaaccg gccgcatccg cttctcgcct gcttacttag ccatatgcct
     2401 gtcgttcggc attcttcccc aagcctgggc gggacacact tatttcggca tcaactacca
     2461 atactatcgc gactttgccg aaaataaagg caagtttgca gtcggggcga aagatattga
     2521 ggtttacaac aaaaaagggg agttggtcgg caaatcaatg acaaaagccc cgatgattga
     2581 tttttctgtg gtgtcgcgta acggcgtggc ggcattggtg ggcgatcaat atattgtgag
     2641 cgtggcacat aacggcggct ataacaacgt tgattttggt gcggaaggaa gaaatcccga
     2701 tcagcaccgt ttttcttacc aaattgtgaa aagaaataat tatcaaaagg atacaaagca
     2761 ttactatcac gaggattatc ataatcctag attaaataaa tttgttactg atgccgctcc
     2821 agtagaaatg accattaata tgaatggtaa tacttatgga aatataactg actatcccga
     2881 tcgtgtgcgt attggatcag ggcgacaata ttggcataaa aaaaatggta accctcaaca
     2941 aatctttggt agttatagtt accgaattgc tggtaatacc cataatcaag tgggaggaag
     3001 tgatggacac gttagtttaa atggggacgt aaaaaaacca aacaactatg gccctcttcc
     3061 tacgggtgtt tcatttggag atagtggttc tccaatgttt atttatgatg caaaaaaaca
     3121 aaaatggcta atcaatggtg tattaagaga aggtaatcca taccaaggaa cacacaatgg
     3181 ctatccaatt gtacgtaaag cctatttaac aaaaatccaa gatggagata ctggagcgag
     3241 tatttatgat atggtgggtg gggcttctta tctgtgggaa gctaaagaat taggaaaaag
     3301 cactattaac agaaaacaca ttacttttgg agaatatgtt gaaggcggag ttggtaaact
     3361 tactttgaaa tcaaatattg atcaaggtgc aggcggtttg tattttgagg gagattttat
     3421 agtttcgcct acccaaaatg aaacatggaa aggggcgggt atatctatca gtgatgaaag
     3481 taccataatt tggaaagtca atggcgtggc aaacgaccgc ctgtccaaaa tcggcaaagg
     3541 cacgctgcac gttcaagcca aaggggaaaa ccaaggctcg atcagcgtgg gcgacggtaa
     3601 agttatttta gatcaacaag cagatgaaaa taataaaaaa caagccttta gtgaaatcgg
     3661 cttggtcagc ggcaggggga cggtgcaact gaatgccgat aatcagttca accccgacaa
     3721 actctatttc ggctttcgcg gcggacgttt ggatttgaac gggcattcgc tttcgttcca
     3781 ccgtattcaa aataccgatg aaggggcgat gattgtcaac cacaatcaag acaaagaatc
     3841 caccgttacc attacaggca ataaagatat tgctacaacc ggcaataaca acagcttgga
     3901 tagcaaaaaa gaaattgcct acaacggttg gtttggcgag aaagatacga ccaaaacgaa
     3961 cgggcggctc aaccttgttt accagcccgc cgcagaagac cgcaccctgc tgctttccgg
     4021 cggaacaaat ttaaacggta acatcacgca aacaaacggc aaactgtttt tcagcggcag
     4081 accgacaccg cacgcctaca atcatttagg aagcgggtgg tcaaaaatgg aaggtatccc
     4141 acaaggagaa atcgtgtggg acaacgactg gatcaaccgc acgtttaaag cggaaaattt
     4201 ccatattcag ggcgggcagg cggtgatttc ccgcaatgtt gccaaagtgg aaggcgattg
     4261 gcatttgagc aatcacgccc aagcagtttt tggtgtcgca ccgcatcaaa gccacacaat
     4321 ctgtacacgt tcggactgga cgggtctgac aaattgtgtc gaaaaaacca ttaccgacga
     4381 taaagtgatt gcttcattga ctaagaccga catcagcggc aatgtcagcc ttgccgatca
     4441 cgctcattta aatttcacag ggcttgccac actcaacggc aatcttagtg caaatggcga
     4501 tacacgttat acagtcagcc acaacgccac ccaaaacggc gaccttagcc tcgtgggcaa
     4561 tgcccaagca acatttaatc aagccacatt aaacggcaac acatcggctt cgggcaatgc
     4621 ttcatttaat ctaagcgacc acgccgtaca aaacggcagt ctgacgcttt ccggcaacgc
     4681 taaggcaaac gtaagccatt ccgcactcaa cggtaatgtc tccctagccg ataaggcagt
     4741 attccatttt gaaagcagcc gctttaccgg acaaatcagc ggcagcaagg atacggcatt
     4801 acacttaaaa gacagcgaat ggacgctgcc gtcaggcacg gaattaggca atttaaacct
     4861 tgacaacgcc accattacac tcaattccgc ctatcgccac gatgcggcag gggcgcaaac
     4921 cggcagtgcg acagatgcgc cgcgccgccg ttcgcgccgt tccctattat ccgttacacc
     4981 tccggcttcg gcagaatccc atttcaacac gctgacggta aacggcaaat tgaacggtca
     5041 gggaacattc cgctttatgt cggaactctt cggctaccgc agcgacaaat tgaagctggc
     5101 ggaaagttcc gaaggcactt acaccttggc tgtcaacaat accggcaacg aacctgcaag
     5161 cctcgaacaa ttgacggtag tggaaggaaa agacaacaaa ccgctgtccg aaaaccttaa
     5221 ttttaccctg caaaacgaac acgtcgatgc cggcgcgtgg cgttaccaac tcatccgcaa
     5281 aggcggcgag ttccgcctgc ataatccggt caaagaacaa gagctttccg acaaactcgg
     5341 caaggcagaa gccaaaaaac aggcggaaaa agacaacgcg caaagccttg acgcgctgat
     5401 tgcggccggg cgcgatgccg tcgaaaagac agaaagcgtt gccgaaccgg cccggcaggc
     5461 aggcggggaa aatgtcggca ttatgcaggc ggaggaagag aaaaaacggg tgcaggcgga
     5521 taaagacacc gccttggcga aacagcgcga agcggaaacc cggccggcta ccaccgcttt
     5581 cccccgcgcc cgccgcgccc gccgggattt gccgcaaccg cagccccaac cgcagcccca
     5641 accgcagcgc gacctgatca gccgttatac caatagcggt ttgagtgaat tttccgccac
     5701 gctcaacagc gttttcgccg tacaggacga attggaccgc gtatttgccg aagaccgccg
     5761 caacgccgtt tggacaagcg gcatccggga caccaaacac taccgttcgc aagatttccg
     5821 cgcctaccgc caacaaaccg acctgcgcca aatcggtatg cagaaaaacc tcggcagcgg
     5881 gcgcgtcggc atcctgtttt cgcacaaccg gaccgaaaac accttcgacg acggcatcgg
     5941 caactcggca cggcttgccc acggcgccgt tttcgggcaa tacggcatcg acaggttcga
     6001 catcggcatc agcgcgggcg cgggttttag cagcggcaac ctttcagacg gcatcggagg
     6061 caaaatccgc cgccgcgtgc tgcattacgg cattcaggca cgataccgcg ccggtttcgg
     6121 cggattcggc atcgaaccgc acatcggcgc aacgcgctat ttcgtccaaa aagcggatta
     6181 ccgctacgaa aacgtcaata tcgccacccc cggccttgcg ttcaaccgct accgcgcggg
     6241 cattaaggca gattattcat tcaaaccggc gcaacacatt tccatcacgc cttatttgag
     6301 cctgtcctat accgatgccg cttcgggcaa agtccgaaca cgcgtcaata ccgccgtatt
     6361 ggctcaggat ttcggcaaaa cccgcagtgc ggaatggggc gtaaacgccg aaatcaaagg
     6421 tttcacgctg tccctccacg ctgccgccgc caaaggcccg caactggaag cgcaacacag
     6481 cgcgggcatc aaattaggct accgctggta acaccggata tgccgaaagg ggtttgacga
     6541 tgccgccgtg cgctgtcaaa ccctttttct gccgccgcct tgtgcctgct ccattgtctg
     6601 ataaccgtca aattgatttt tagcccatgt ttggtgggtc ggggaaatct atatcttcgt
     6661 ccgtgccgaa atagtctatg cccgatatac aattttgata cacaaacttg gaaatatcgg
     6721 tatcgtcgcc ggagcgatag aatgcggaca gtttttcatt gaattgcggc atctgcattt
     6781 cggagatttc caagatgccg cagccccccg ccatcatcag accgttggca aataaggttg
     6841 ccgttcgttt gttgccgtcc caaaaaacct gctgccgcat acaataaagc ataaaacgga
     6901 cggctgcctc cgtcgtcgaa ccgctttgca gtccgatatt ttgtaacacg cgggccactt
     6961 caatttcctt cactggatt
//
LOCUS       DAWFJW010000101         6970 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFJW010000101 DAWFJW010000000
VERSION     DAWFJW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.101"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            56..1294
                     /gene="mtrC"
                     /locus_tag="WGR19_001683"
     CDS             56..1294
                     /gene="mtrC"
                     /locus_tag="WGR19_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0799175.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            1306..4509
                     /gene="mtrD"
                     /locus_tag="WGR19_001684"
     CDS             1306..4509
                     /gene="mtrD"
                     /locus_tag="WGR19_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0799176.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            4564..5967
                     /gene="mtrE"
                     /locus_tag="WGR19_001685"
     CDS             4564..5967
                     /gene="mtrE"
                     /locus_tag="WGR19_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0799177.1"
                     /translation="MDTTLKTTLTSVATAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(6159..6395)
                     /locus_tag="WGR19_001686"
     CDS             complement(6159..6395)
                     /locus_tag="WGR19_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0799178.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
     gene            complement(6335..6634)
                     /locus_tag="WGR19_001687"
     CDS             complement(6335..6634)
                     /locus_tag="WGR19_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0799179.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
BASE COUNT         1569 a         1829 c         1955 g         1617 t
ORIGIN      
        1 aatgcctgtt gattggcaaa accctttaca tcaaaccaga cagataggat aaataatggc
       61 tttttatgct tttaaggcga tgcgtgcggc cgcgttggct gccgccgttg cattggtact
      121 gtcgtcttgc ggtaaaggcg gagacgcggc gcagggcggg cagcctgctg gtcgggaagc
      181 ccctgcgccc gtcgtcggtg tcgtaaccgt ccatccgcaa accgtcgcgt tgaccgtcga
      241 gttgccgggg cgtttggaat cgctgcgtac cgccgatgtc cgcgcccaag tcggcggcat
      301 catccaaaaa cgcctgttcc aagaaggcag ttatgtccgt gccggacagc cgctgtatca
      361 gatcgacagt tccacttatg aagcaggtct ggaaagcgcg cgcgcgcaac tggcaacggc
      421 tcaggcaacg cttgccaaag cggatgcgga tttggcgcga tacaagcctt tggttgccgc
      481 cgaagccgtc agccggcagg aatacgatgc tgcggtaacg gcgaaacgtt ctgccgaggc
      541 aggtgtcaaa gcagcacagg cggcgatcaa atctgccggc atcaatctga accgttcgcg
      601 cattaccgcg ccgatttccg gctttatcgg tcagtccaaa gtttccgaag gtacgctgtt
      661 gaatgcgggc gatacgaccg tgctggcaac catccgccaa accaatccga tgtatgtgaa
      721 cgttacccag tctgcatccg aagtgatgaa attgcgccgt cagatagccg aaggcaaact
      781 gctggcggcg gatggtgtga ttgcggtcgg catcaaattt gacgacggca cagtttaccc
      841 tgaaaaaggc cgcctgctgt ttgccgatcc gaccgtcaac gaatcgaccg gtcagattac
      901 cctgcgcgcc gccgtaccga acgatcagaa tatcctgatg cccggcctgt atgtgcgcgt
      961 gctgatggac caagtggcgg tggataacgc atttgttgtg ccgcagcagg cggtaacgcg
     1021 cggtgcgaaa gataccgtga tgattgtgaa tgcccaaggc ggtatggaac cccgcgaggt
     1081 aacggttgcg caacagcagg gtacgaattg gattgttacg tcgggtctga aggacgggga
     1141 caaggtggtt gtggaaggca tcagtatcgc cggtataacg ggtgcgaaaa aggtaacgcc
     1201 caaagaatgg gcgccgtctg aaaaccaagc cgccgcgcct caatccggcg ttcagacggc
     1261 atctgaagcc aaaactgctt ctgaagcgga ataaggaagg catcgatggc taaatttttt
     1321 atcgaccgcc ctatttttgc gtgggttatt tcgattttca ttattgcggc gggtattttc
     1381 ggcatcaaaa gcctgcctgt ttcgcaatat ccgtctgtcg ccgccccgac cattactttg
     1441 cacgccactt atccgggtgc gtccgcgcag gtaatggaag acagcgtgct ttccgtgatc
     1501 gagcggaata tgaacggcgt ggaaggtttg gattatctgt ccacttccgc cgattcgagc
     1561 ggcagcggca gcgtgagcct gacctttacg cccgataccg acgagaatct ggcgcaggtg
     1621 gaagtgcaga acaagctttc cgaagtattg agcacgctgc cggcaaccgt ccagcaatac
     1681 ggcgtaaccg tatccaaggc gcgttccaat ttcctgatga ttgtgatgct ttcgtcggat
     1741 gtgcagtcaa ccgaagagat gaacgactac gcgcagcgta atatcgttcc cgaactgcag
     1801 cgtatcgaag gcgtggggca ggtacgcctg ttcggcgcgc aacgcgcgat gcggatttgg
     1861 gttgatccta agaaactgca aaactacaat ttgtcgtttg ccgatatcgg cagtgcgctg
     1921 tccgcccaga acgtccagat ttcagcgggt tctatcggtt cgcttcccgc cgttcgcgga
     1981 cagacggtta cggctaccgt aacggcgcaa gggcagttgg gtacggcaga agaattcggc
     2041 aacgtcatcc tccgcgccaa taccgacggt tctaatgttt acctgaagga tgtggcaagg
     2101 gtcggactgg gtatggaaga ctattcttcc tcaacccgtc tgaacggtgt aaataccacc
     2161 ggtatggcgg tgatgctgtc caacagcggc aatgcgatgg cgacggcaaa ggcggttaaa
     2221 gaacgcatgg cgacgttgga aaaatacttt cctcagggta tgagctggaa aaccccttac
     2281 gatacttcca aattcgtcga aatttcgatt gaaaaagtga ttcatacttt aatcgaagcg
     2341 atggtgctgg tgtttgtcgt aatgtatctc ttcctgcaaa acatccgtta tacgctgatt
     2401 ccgaccatcg tcgtaccgat ttcgctgttg ggcggtttcg ccttcatctc ttatatgggc
     2461 atgtcgatta acgtactgac catgtttgcg atggttttgg tcatcggcat cgtggtcgat
     2521 gacgcgattg tggtggttga aaacgtcgag cgcattatgg cgggtgaagg cttgccgccc
     2581 aaagaagcga ccaaaaaagc gatgggtcag atttcgggcg cggtcatcgg tattaccgcc
     2641 gttctgattt ccgtgttcgt accgttggcg atgttcagcg gggcgacggg caatatttac
     2701 aaacagtttg ccctgacgat ggcgtcatca atcgcattct ccgccttcct tgcgctgacc
     2761 cttacccctg ctttgtgtgc cacaatgttg aagacaatcc cgaaagggca tcacgaagag
     2821 aaaaaaggtt tcttcggctg gtttaacaag aaattcaaca gttggacgca cggttacgaa
     2881 ggccgggttg ccaaagtgct gcgtaagact ttccgcatga tggttgtcta tatcggcttg
     2941 gcggttgtgg gcgtgttcct gtttatgcgc ctgccgactt cgttcctgcc gaccgaagac
     3001 caaggcttcg tcatggtcag cgtacaactg cctgcggggg cgacccaaga gcgcaccaat
     3061 gcgactttgg cgcaagttac ccaactggcg aaaagcattc ctgaaataga aaacatcatt
     3121 accgtttccg gcttcagctt ttcgggcagc ggtcagaata tggcgatggg ttttgccata
     3181 ttgaaagatt ggaacgagcg taccgcgccc ggcagcgatg ccgttgcgat tgccggcaag
     3241 ctgacgggta tgatgatggg gacgcttaaa gacggttttg gcatcgccgt cgtcccgcct
     3301 ccgattctgg agttgggcaa cggttcgggc ctgagcatca acctgcaaga ccgcaacaat
     3361 accggccata ccgcattgct ggcgaagcgc aacgagttga ttcagaaaat gcgtgccagc
     3421 ggtttgtttg accccagcac cgtccgtgcc ggcggtttgg aagactcgcc gcagttgaaa
     3481 atcgacatca accgtgccgc cgcggcggcg caaggcattt cgtttgccga catccgcacc
     3541 gcattggcaa gcgcgctgag ttcgtcttat gtcagcgact tcccgaacca aggccgtctg
     3601 caacgcgtga tggtgcaggc cgacgaggat gcccgtatgc agcctgccga tattttgaac
     3661 ctgaccgtgc cgaacaaatc cggcgtcgcc gtaccgcttt ccaccattgc tactgtttct
     3721 tgggaaaacg gtacggaaca gagcgtacgc ttcaacggtt atccttcgat gaagctgtcc
     3781 gcttcgcctg caaccggcgt ttccaccggg caggctatgg cggcggttca aaaaatggtt
     3841 gacgaattgg gcggcggtta cagcttcgaa tggggcggac agtcgcgcga agaggcaaaa
     3901 ggcggctcgc aaaccctgat tttgtacggt ttggcggttg cagccgtatt tttggtactt
     3961 gccgcgcttt atgaaagctg gtcgattccg ctggcggtca tccttgtgat tccgttgggt
     4021 ttgatcggtg cggctgcggg cgtaaccggg cgcaacctgt ttgaaggact gttgggcagc
     4081 gttccctcgt tcgccaacga catctacttt caagtcggtt tcgttaccgt gatgggtttg
     4141 agtgcgaaaa acgcgatttt gattatcgag tttgccaaag accttcaagc gcaagggaaa
     4201 agcgcggttg aagccgcttt ggaagcagcc cgcctgcgtt tccgtccgat tatcatgacc
     4261 tcgtttgcct ttattttggg cgtggtcccc ctgtatattg ccggcggtgc aagctccgcc
     4321 agccagcgcg ccatcggtac aaccgtattc tgggggatgc tcatcggcac gcttttgtcc
     4381 gtgttccttg ttccgctttt ctatgtggtg gtgcgcaaat tcttcaaaga aaccgcgcac
     4441 gaacacgaaa tggcagtaaa acacgccgcc gaagcgggca tcaccggttc ggacgacagc
     4501 caacattaag caaccatgcc gtctgaacgc ccacgggttt tcagacggca tcaggacttt
     4561 tttatggata ctacattgaa aaccaccttg acttctgttg caacagcctt cgcattatcc
     4621 gcctgcacca tgattcccca atacgagcag cccaaagtcg aagttgccga aacgtttaaa
     4681 aacgataccg ccgacagcgg catccgtgcg gtcgatttag gttggcatga ctattttgcc
     4741 gacccgcgcc tgcaaaagct gatcgacatc gcactcgagc gcaataccag tttgcgtacc
     4801 gccgtattga acagcgaaat ctaccgcaaa caatacatga ttgagcgcaa caacctcctg
     4861 cccacgcttg ccgccaatgc gaacggctcg cgccaaggca gcttgagcgg cggcaatgtc
     4921 agcagcagct acaatgtcgg actgggtgcg gcatcttacg aactcgacct gttcggacgc
     4981 gtccgcagca gcagcgaagc agcactgcaa ggctattttg caagtgtcgc caaccgcgat
     5041 gcggcacatt tgagcctgat tgccaccgtt gccaaagcct atttcaacga acgttatgcc
     5101 gaagaagcga tgtctttggc gcagcgtgtt ttgaaaacgc gcgaggaaac ctacaagctg
     5161 tccgaattac gttacaaggc aggcgtgatt tccgccgtcg ccctacgtca gcaggaagcc
     5221 ctgatcgaat ctgccaaagc cgattatgcc catgccgcgc gcagccgcga acaggcgcgc
     5281 aatgccttgg caaccttgat taaccaaccg atacccgaag acctgcctgc cggtttgccg
     5341 ctggacaagc agttttttgt tgaaaaactg ccggccggtt tgagttccga agtattgctc
     5401 gaccgtcccg acatacgcgc cgccgaacac gcgctcaaac aggcaaacgc caatatcggt
     5461 gcggcacgcg ccgccttttt cccatccatc cgcctgaccg gaaccgtcgg tacgggttct
     5521 gccgaattgg gtgggttgtt caaaagcggc acgggcgttt ggtcgttcgc gccgtctatt
     5581 accctgccga tttttacctg gggtacgaac aaggcgaacc ttgatgtagc caagctgcgc
     5641 caacaggcac aaatcgttgc ctatgaatcc gccgtccaat ccgcatttca agacgtggca
     5701 aacgcattgg cggcgcgcga gcagttggat aaagcctatg acgctttaag caaacaaagc
     5761 cgcgcctcta aagaagcgtt gcgtttggtc ggtctgcgtt acaaacacgg cgtatccggc
     5821 gcgctcgact tgctcgatgc ggaacgcagc agctattcgg cggaaggtgc ggctttgtcg
     5881 gcacaactga cccgcgccga aaaccttgcc gatttgtaca aggcactcgg cggcggattg
     5941 aaacgggata cccaaaccga caaataaccg gtcgggcaat aaaatgccgt ctgaaacgat
     6001 aaggtttcag acggcatttc tttctttata gtggattaaa aaaaaccagt acagcgttgg
     6061 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt tgtactattt
     6121 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atgtttgacg
     6181 gttgcggtca ggctgcgcgg cgtgccgtaa acgtgaatgt cgggcatagg gcggtagcgt
     6241 ttgttgaaca cgttttcaaa gtccaaaccg atgcgggtgg atttgccgat tttgtaatgc
     6301 gcggtcaaat ccagcgtggc gtaggggcgt tgggtcagtg cggctgcggc ggcagggaag
     6361 aaaccgccat acccgccggc atcccgaagc cgaatacggc aacggcaagc gcaatcagca
     6421 gcgtataaag ccccgcctga tagcctttca gcttcaggac ggtcagcgcg gcaaagaaaa
     6481 agacgatggg taagagtgcg gcggcggcag tcagatacag gctgccgccg attgccgtgt
     6541 agttttgaac ccaagtttcc ataattgaac atctccgaaa atatttttct aatcgtcggc
     6601 aatagtggtc aaaccaatta aagcaacgtt gcattacttt gcgaaacttt aatattcagg
     6661 tcaatagaat ggattaaatt taaaccagta cggcgttgcc tcgccttacc gtactatctg
     6721 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata tatttttggg
     6781 cggttcggca gatttgaatc ggagctttcg tttaaatcct gtcaaaacaa atatttgtat
     6841 aaacaaaaat tgtagtttgg tgtagttttt tcttgtgttt cgggggcggt gcgaggtaaa
     6901 tgccgttgac ggcgggaacg gcgtgtcatt agaatccgcc ctgattggtc agtccaattt
     6961 gatgtttgat
//
LOCUS       DAWFJW010000102         6949 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFJW010000102 DAWFJW010000000
VERSION     DAWFJW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.102"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WGR19_001688"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WGR19_001688"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0799180.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAGLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="WGR19_001689"
     CDS             1102..1512
                     /locus_tag="WGR19_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0799181.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFGRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGSRGLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /locus_tag="WGR19_001690"
     CDS             1533..3596
                     /locus_tag="WGR19_001690"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0799182.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKSVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEQTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3613..4659
                     /locus_tag="WGR19_001691"
     CDS             3613..4659
                     /locus_tag="WGR19_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0799183.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRPEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4701..5528
                     /locus_tag="WGR19_001692"
     CDS             4701..5528
                     /locus_tag="WGR19_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0799184.1"
                     /translation="MQNHVISLASAAERRAHIAATFGRHGIPFQFFDALMPSEELEQA
                     MAELVPGLSAHPYLSSVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGEGAEQFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPSEGLHPVDWMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5540..5635
                     /locus_tag="WGR19_001693"
     CDS             5540..5635
                     /locus_tag="WGR19_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0799185.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5677..6519
                     /locus_tag="WGR19_001694"
     CDS             5677..6519
                     /locus_tag="WGR19_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0799186.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLA
                     EDGWLKERFDPDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1790 a         1844 c         1815 g         1500 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ttgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcact cggccccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgcc aatatccaag acctctatct cgactctctg cgcgaattgg
      421 gcatcgatcc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacgcccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgct ggacggcaat accgtaagct acggcgacgt gtaccaccaa aacgaagtcg
      721 agcaatccac ctacaacttc gaatacagcg atgccgactg gctgctgcgc caattcaacg
      781 actacgaagc gcaagccaaa cgcctgctcg ccgaagaaaa cgccggcctc gccctgcctg
      841 cctacgagct cgtcctcaaa gcggggcata cgttcaacct cttggacgcg cgcggcgcga
      901 tttccgtaac cgagcgagca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgtcgaaagc cgcgaaaaac tgggctttcc cttaatgaag gcaaatgccg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcccgat atggcagccg tccgtaaacc
     1081 attatatatt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cggccgcttt
     1261 atggacgagc tgattgcgta tcttgcggca aacggcttgg aaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccggctccc gaggcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggt cgcgaagatg tcgccacttt gtacgccacc
     1441 ccgctggttg atggttttta tggggatgac ggctgggaaa attacagtga aacctataaa
     1501 caggcagatt aaccaaattt aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg ccaaaagctc tgaataatct gggcaaccat tttgccgccg
     1621 ccgttgccga aggcttggaa aaggcgcaac tggttgacgg cgcagccgaa tttacagctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt caaatccgtt caagccgatc
     1741 agaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggttttgca cgcggtgcgg gggcgaaaat cgaagatttg accatcgtcc
     1861 acgacggcaa gcaggatgtg tacgcctacg aatacgtcca aaccggcaag ccgctgggcg
     1921 gacttttgga agacattatc aatcaagtgg ttaagaaact gccgattccc aaagtgatgc
     1981 gttggggcag cagcacattt accttcgtgc gccctgttca cgggctgatt gtgctgcacg
     2041 gcggcgacat cgtaaacgtc agcgttttgg gtctgcaaag cagcaatcaa accttgggac
     2101 accgcttctt gtccaacggc gaaatcacca ttgaaaacgc cgacagctac gccgaacaaa
     2161 tgcgcgggca aggcaaagtc gtcgcttcgt ttgccgagcg caaagccgcg attcagacgg
     2221 cattggaagg gcaggcgcgc agtctgaacg ccgccgtcgc cgccgatgaa gcattgttgg
     2281 acgaagtaac cgcgctggtc gaatggcctg ttgttttgga agccggtttt gaggaacatt
     2341 tcctcgccgt gccgcaggaa tgcttgattt taacgatgca gcaaaaccaa aaatactttc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtttcc aacctgcaaa
     2461 ccgaagaccc gtcgcacatc atccgaggca acgaacgcgt tttgcgcgcg cgcctgtctg
     2521 atgccgagtt cttctacaaa caagaccaaa aggcgacttt ggaaagccgc ctgcccaagc
     2581 tggcaaacgt ggtttatcac aacaaaatcg gttcgcaagc cgaacgcatc gaacgcctgc
     2641 aaagcattgc cgcccacatc gctaaagcgt tgggcgcaga tgccgccgcc gccgaacgcg
     2701 ccgcgcgtct ggcaaaagcc gacttggtaa ccgaaatggt cggcgagttc cctgaattgc
     2761 aaggcaccat gggcaaatac tatgcccgtt tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca gcactatcag ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg
     2881 ccgccgccgt ggcactggcg gacaagctgg aaaccctggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgataaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtacggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg cttgctcaac gagcaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgttgccgcc gtattggcga
     3181 aacagccgcg ccgtttggac gacgtagtgg cgaaactgca agccgttgcc gcgttcaaac
     3241 aactgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaat ctgctgaaaa
     3301 aagccgatgc cgagttgggc gcggttaacg aaagcctgct gcaacaggac gaagaaaaag
     3361 ccctctttgc cgccgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaagcc gcaagtcgat gccttctttg
     3481 acggcgtgat ggtgatggcg gaagatgccg ccgtaaaaca aaaccgtttg aacctgttga
     3541 accgtttggc agagcagatg aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtacagtcc aaatgccgtc tgaagccttc agacggcatc gtgcctatcg ggagaataaa
     3661 ttgcagcctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata tttcgcccaa
     3721 tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg agattttgat tgtcgatgac
     3781 ggctcgacag acggtacgct tgccattgcc aaggattttc aaaagcggga cagccgtatc
     3841 aaaatccttg cacaagctca aaattccggc ctgattccct ctttaaacat cgggctggac
     3901 gaattggcaa agtcaggaat gggggaatat attgcacgca ccgatgccga cgatattgcc
     3961 gcccccgact ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcatcgcg
     4021 atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg gcaaccggct ggcgcggcat
     4081 cacaggcacg gcaaaatttg gaaaaagccg acccgacccg aagatattgc cgactttttc
     4141 cctttcggca accccataca caacaacacg atgattatga ggcgcagcgt cattgacggc
     4201 ggtttgcgtt acaacaccga gcgggattgg gcggaagatt accaattttg gtacgatgtc
     4261 agcaaattgg gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc
     4321 aatcaggttt catccaaata cagcatccgc caacacgaaa tcgcgcaagg catccaaaaa
     4381 accgccagaa acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa
     4441 taccgccaaa taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat
     4501 tttgaacgcg cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gctgcccgcc
     4561 ggcgcgtggc tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa
     4621 tacttcggca ttttgcaccg attgctgaaa aaccgttgaa aaacgccgct ttatccaaca
     4681 gacaaaaaac aggataaatt atgcaaaacc acgttatcag cttggcttcc gccgcagaac
     4741 gcagggcgca cattgccgca accttcggca ggcacggcat cccgtttcag tttttcgacg
     4801 cactgatgcc gtctgaagaa ctggaacagg cgatggcgga actcgtcccc ggcttgtcgg
     4861 cgcatcccta tttgagcagt gtggaaaaag cctgctttat gagccacgcc gtattgtgga
     4921 aacaggcact ggatgaaggt ctgccctatg ttgccgtatt tgaggacgac gttttactcg
     4981 gcgaaggcgc ggagcagttc cttgccgaag acgcttggct gcaagaacgc tttgaccccg
     5041 attccgcctt tatcgtccgt ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
     5101 gtgtggcgga ctactgcggg cgtgcctttc cgctgttgga aagcgaacac tgggggacgg
     5161 caggttatat tatttcccga aaagcgatgc ggtttttcct ggacaggttt gccgccctgc
     5221 cgtctgaagg gctgcacccc gtcgattgga tgatgttcgg caaccctgat gacagggaag
     5281 gaatgccggt ttgccagctc aatcccgcct tgtgcgccca agagctgcat tatgccaagt
     5341 ttcacgacca aaacagcgca ttgggcagcc tgattgaaca cgaccgcctc ctgaaccgca
     5401 aacagcaaag gcgcgattcc cccgccaaca cattcaaaca ccgcctgatc cgcgccttga
     5461 ccaaaatcag cagggaaagg gaaaaacgcc ggcaaaggcg cgaacagttc attgtgcctt
     5521 tccaataaaa ggaggagaga tggacatcgt atttgcagca gacggcagga tgcggcggct
     5581 gtttaccttg aggcaatact tcggcatttt gcgccgattg ctgaaaaacc gttgaaaaac
     5641 gccgctttat ccaacagaca aaaaacagga taaattatgc aaaaccatgt tatcagctta
     5701 gcttccgccg cagaacgcag ggcgcacatt gccgatacct tcggcaggca cgacattccg
     5761 tttcagtttt tcgacgcact gatgccgtct gaagaactga atcggatgat ggcggaattg
     5821 gtgccgggat tggcaaaaca gcacctgttg agcgaagtgg aaaaagcctg ctttatgagc
     5881 cacgtcgtat tgtggaaaca ggcattggac gaaggcgtac cgtatgttgc cgtatttgag
     5941 gatgatgtct tactcggcga aggggcggag aaattccttg ccgaagacgg ctggctaaaa
     6001 gagcggtttg acccggattc cgcctttatc gtccgtttgg aaacgatgtt tgcgaaagtt
     6061 attgtcagac cggataaagt cctgaattat gagaaccggt catttccttt gctggagagc
     6121 gaacattggg ggacggctgg ctatatcatt tcgcgtgagg cgatgcggtt tttcttggaa
     6181 aggtttgccg ttttgccggc agagtggatt aaagcggtag attggatgat gtttacttat
     6241 ttctttgata aggaggggat gcctgtttat caggttaatc ccgccttatg tacccaagaa
     6301 ttgcattatg ccaagttcct cagtaaaaac agtatgttgg gtagcgattt ggaaaaagat
     6361 agggaacaag aaagaagaca ccgccgttcg ttgaaggtaa tgtttgactt gaagcgtgct
     6421 ttgggtaaat tcggtagaga aaagaagaaa agaatggagc gtcaaaggca ggcggagctt
     6481 gagaaagctt acggcaggcg ggtcatatcg ttcaaatagt ttgtgtaaaa tatagcggat
     6541 taaaattaga aatggataca ctgtcattcc cgcgcaggcg ggaatccagt ccgttgagtt
     6601 tcagttattt ccgataaatc accgcaacgt taaatttcta gattcccgct tttgcgggaa
     6661 tgacggcggg ggatttctgt ttttccgaca aattcctaaa acttaaaatt ccgttattcc
     6721 cgcgaaaaca gaaaaccaaa aatagaaacc taaaatctcg tcattcccgc aaaaacagaa
     6781 aaccaaaaaa cagcaaccta aaattcgtca ttcccgcgca ggcgggaatc cagtccgttc
     6841 ggtttcgctt gttttgggtt tcgggtaact tccacttcgt cattcccgcg aaagtgggaa
     6901 tccggttcgt tgagtttcag tcatttccga taaatcaccg caacattaa
//
LOCUS       DAWFJW010000103         6935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFJW010000103 DAWFJW010000000
VERSION     DAWFJW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.103"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            978..1424
                     /gene="yciA"
                     /locus_tag="WGR19_001695"
     CDS             978..1424
                     /gene="yciA"
                     /locus_tag="WGR19_001695"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0799187.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAQGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(1475..2296)
                     /locus_tag="WGR19_001696"
     CDS             complement(1475..2296)
                     /locus_tag="WGR19_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0799188.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            2415..2873
                     /locus_tag="WGR19_001697"
     CDS             2415..2873
                     /locus_tag="WGR19_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0799189.1"
                     /translation="MNIQTRLAATALALFLSACGNGGAPAQPKGEISENRTTAFKSMM
                     PDFSRMGKMVKGEEPYDVGKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDSA
                     KFKAEEEKFAAAVEKLNAAAQTGKLDEIKAAYGETGSSCKSCHDSFRAPE"
     gene            complement(3180..4295)
                     /gene="lst"
                     /locus_tag="WGR19_001698"
     CDS             complement(3180..4295)
                     /gene="lst"
                     /locus_tag="WGR19_001698"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0799190.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEW
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN"
     gene            complement(4385..4543)
                     /locus_tag="WGR19_001699"
     CDS             complement(4385..4543)
                     /locus_tag="WGR19_001699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799191.1"
                     /translation="MAYFGGFVRALFDLGVFERGGRKGGNGFPASGGGAAPDLRRHIF
                     GEIDFNIR"
     gene            4612..6837
                     /locus_tag="WGR19_001700"
     CDS             4612..6837
                     /locus_tag="WGR19_001700"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0799192.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFTRHAGIEVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKTVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPDATSVSILFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLTEQVQDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAASVNVNNGFGNLLANLDKLDTDTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGRVQVVDEAGNVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     EPDTDGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAKSLAENEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQALNA
                     L"
BASE COUNT         1819 a         2065 c         1576 g         1475 t
ORIGIN      
        1 attcccgata acagcgcaat cttgaaaccc gtcattcccg acaacaccgt aatctcgaaa
       61 ttcgtcattc ccgcgcaggc gggaatccag ccccctgacg cggcgggaat ctatcggaaa
      121 tgactgaaac cccgcgttct agattcccac ttccgtggga atgacggttc agttgcgctc
      181 cgacaacacc gtaatctcaa aacccgtccg acaacaccgc aatcttgaaa ttcgtcattc
      241 ccgcacaggc gggaatccag acctgtccgc acagaaactt atcggataaa aacagttgcc
      301 caaaccccgc gttctagatt cccactttcg tgggaatgac gggatgcagg tttccgtatg
      361 gatggattcg tcattcccgc gcaggcggga atccagaccc ccgacgcggc gggaatctat
      421 cggaaatgac tgaaaccccg agattctaga ttcccacttc cgtgggaatg acggttcagt
      481 tgcgttccga caacaccgta atcttgaaat ccgtccgaca acaccgtaat cttaaaaccc
      541 gtcattcccg cgcaggtggg aatccagacc ccctgacgcg gcgggaatct atcgaaaatg
      601 actgaaaccc cgagattcta gattcccact ttcgtgggaa tgacgtggtg caggtttccg
      661 tatggatgga ttcgtcattc ccgcgcaggc gggaatccag accccctgac gcggcgggaa
      721 tctatcggaa atgactgaac ccccgagatt ctagattccc actttcgtgg gaatgacggt
      781 tcagttgctt agggtaggat ttggcgggat tggcggactg aagcccactc tacagccccg
      841 ccctacaata cgccttgcga atctgtccca cgcccgccct tgtatgtctc aaatgggtta
      901 aactacgcac ttcccgactt cccgtttcgg gcaggggtgc aggttcgatt tatctttcaa
      961 taaaacaagg aaactttatg cagcacgaag aaggcaaccg ccaacgccct caaggcgaac
     1021 tgctcctgcg taccgtcgct atgccgcgcg ataccaatcc caaccaagac attttcggcg
     1081 gctggattat gtcgcagatg gatttgggcg gcggcatatt ggcggcggaa atcgcacaag
     1141 gacgcatcgt taccgtcgcc gttcaggaaa tgaacttcat ccgcccggtc aaggtcggca
     1201 acgtcgtctg ctgctacggg cattgcgtcc gcgtgggcaa tacttccctc cagcttaaag
     1261 tggaagtctg ggtgaaaact ttgatgaacg attgcgttac cgaagaccga cacctcgtaa
     1321 ctgaagccgt gttcacttat gtcgccatcg atgcggaagg caatccgcgc ccgattccga
     1381 aagaaggcaa ccccaaattg tcgggcttat tgcctactcc gtaaaaatgc ccgtaaaaat
     1441 gccgtctgga attgtcttca gacggcattc cgtatcattt gaaccaagac ctgatgcggg
     1501 aaaacagcga catactctgt ttttcaaagt cttcccgcac ttcgggcacg gcttttttta
     1561 tcactttcat tcctgcaaaa gtctgcgcca cgggcataat ttcaatcgtg ttcacattca
     1621 tatgcgccgg ccggcggtac agccacaatg cggtttccgc aatatcttcg gggcggataa
     1681 attccacacc ctcatacacg cccgccgccc tctcgtcatc gcctttgaag cgcacgttgg
     1741 aaaactccgt attgccgcac aaacccggct cgatattggt aacgcggatg ttcttatccg
     1801 ccaactccgc gcgcaaattc aggctgaact ggcgcacaaa cgccttggtc gccccgtaaa
     1861 cgttgctgcc ggcataagca taattgcctg caatcgaacc caaattcatc acataaccgc
     1921 cgccgcgttc caccatttgc ggcaaaattt tgcgcgtcag gaacgtcaaa cccaaaacat
     1981 tggtttgaat catcgtttcc caatcttcaa aatccgcctt gtccgccgtg tccagaccca
     2041 aagccagccc ggcattgttg atgaggcagt cgatgtcgga aaattcatcg gggatgccgt
     2101 ttaaggcatt ttccaccgat tcgcggcgcg acacgtccat ttccaaaggg taaaacaaag
     2161 cacccaattc atccgccaag gcctgaagcc tgtccgcgcg gcgcgccgca ccgataacgc
     2221 ggtatcccgc cccgacaaac gcgcggcaca tcgcttcgcc gaatcctgcc gaagcaccgg
     2281 taattaaaac agccattgtt tttcctttct ttctcgtttc ccaaacctgt ttcaggtatc
     2341 ataacaccct tcagacggca tcgcgccggc tgaaaacgac aacaaccata ccaccacgac
     2401 aggacacatt caatatgaac attcaaaccc gccttgccgc aaccgccctc gccctattcc
     2461 tttccgcctg cggaaacggc ggcgcgcccg cacagcccaa aggcgaaatt tccgaaaacc
     2521 gcaccaccgc attcaaatct atgatgcccg acttctcgcg tatgggcaaa atggtcaaag
     2581 gcgaagagcc ttacgatgtc ggaaaattca aacaggcggc tgcggcgttt gccgaaagca
     2641 gtaagaaacc gtttacactt tttgagtccg atccgcaagg caacgggcgc gccctgcccg
     2701 ccgtttggtc ggacagtgcc aagtttaaag ccgaagaaga aaaattcgcc gccgccgtcg
     2761 agaaactcaa cgccgccgcc caaaccggca aactggacga aatcaaagcc gcctacggcg
     2821 aaaccggctc aagctgcaaa tcctgccacg acagcttccg cgcgcccgaa taacatccaa
     2881 aacaaaacaa tgccgtctga agccttcaga cggcattttc tttgtcattt cggcacacgc
     2941 aaagcaatgt ttcaacacac aggacggcac atcataaagc accgccctat gtgttgccct
     3001 gatttcgccc ctcccaaata aagtccgatc ctaccgcccc gaaggacgga tgtccgagtg
     3061 gcggggtttc aaccgaaaag gaaacgacga tcaaagatta tcgccaattc aaaaaatacg
     3121 ccatatcctg atttttgcct tactgactcc gccattttct attttgattt actatgcgat
     3181 taatttttat cgtcaaatgt caaaatcggg atgccggatt gggtaaacag ggcatacacc
     3241 ggcttgagcc aataatcttc cggaagggaa gccggtttca atgcgtaaac gtgcacattg
     3301 ggaaaatcct tcatcgtcaa cgccgcaccg ctgaaaaagg tataaatttc atacctcgta
     3361 tgcgggtttt tcttaatttc gcgcaaaata tagtcttcga tgacataggg cgaatttaat
     3421 gtggttacgc cggaaagccc gtaggtttgg cgggggtgcg gtgcgacata ttgtatgttg
     3481 aagttttttg ccgccttttc cgaaatttcc ttcatctcct tgtcaggcga acccaaaagt
     3541 atccgcaccg tgccgcccgt ttcgtccccc gccttcagtt cggacgcatc gaacagcggc
     3601 aggtaagtca tcttgcggcg accgtcgtcc ataatgtttt tcaaaccctt gaatatcgtg
     3661 taatgctcgt cggaagcatt acgggttttg gcgatgctcc aatctccgat catcatccgg
     3721 gcaaaattcc gcttgatcgt cccgtttaca gaaaactcat cacccaaata gctgctgctt
     3781 tgaattaaat tgattgtccc gtcgtcaaag gttttgattt ccgcatccgg gtaagtgctc
     3841 aaaaaggcgg caatgctgac tttttccaaa cttgccaaat aaatccgctt gaccttcggc
     3901 agcagcattg ccttcacctt cagctccgcc atcgtcggaa tgaaattaaa cgatttgttc
     3961 aaaccgtagg gcaggtggaa aaaatacgcc cactccgcct tatcctttat ctgattgaaa
     4021 taataatcgt atttttcatt cctgttttca gacatcagca ccacataaaa ccgctccccc
     4081 ggatgctgcg ccataatcct ttctgccacc ttcatctgca atatggtata gcagaaaatc
     4141 agattgaccg gttccccctc ttcattgaag agtttgtcct tcagcaggga aaccgcattc
     4201 ctttccccct gatttacccg gtcaaatgta taaaatatcc cgaaacaaaa aacaatcagg
     4261 cacaacacgg tcaaacaagc ctttttcaag cccatatccc taaaactcca ttccgacaaa
     4321 ttgaacatac gccccgagtg tcccgggacg gaaaaatgcg gaattttagc agatattccg
     4381 acagttaccg aatattaaaa tcaatttcac cgaaaatgtg ccgccgcaaa tcaggcgcag
     4441 ctcctccgcc ggacgcggga aaaccattcc cccctttcct gccgccgcgt tcaaacacgc
     4501 caagatcgaa caaagcgcga acaaagccgc caaaatatgc cataatagcc gcgcacattc
     4561 ctcgcgcaat gtgttccaaa agaaaaaacc aacctacaag agaataatcc catgactcaa
     4621 aaatccacca ttgtttacac ccataccgac gaagcccccg cactggcgac ccaatccctg
     4681 ctgccgattg tgcaggcgtt tacccgccac gccggcatcg aggtcaaaac tgccgacatt
     4741 tccctgtccg gccgcatttt ggcggcgttt ccagaatacc tgaccgaagc gcaacgcgta
     4801 cctgatgcgc ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa cgtaatcaaa
     4861 ctgccgaaca tcagcgcatc cgtacctcaa ctgactgccg cgattaaaga attgcagtct
     4921 aaaggctttg ccgttcccga ctatcccgcc gacccgcaaa ccgacgaaga aaaaaccgtg
     4981 cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg cgaaggcaac
     5041 tccgaccgcc gcgcgcctaa agcagtgaaa aactttgcca aaaaaaatcc gcacagtatg
     5101 ggggcatgga caaaagactc caaaacccac gtcgccacga tgcaaagcgg cgattttttc
     5161 cataacgaac aatccgttac cgtacccgat gcgacttccg tatccatctt attcaccgac
     5221 aaacaaggca acaaaaaaga gctgcgcgcg cctgttgccc tgaaagccgg cgaaatcatc
     5281 gacgcgaccg tgatgagcaa aaaagccctg ctcgccttcc ttaccgaaca agtgcaagac
     5341 gcgaaagcta aaggcgtatt gttctcgctg catatgaaag ccacgatgat gaaagtgtcc
     5401 gacccgatta tcttcggaca cgccgtcaaa gtgttcttcg cgcctgtctt tgagaaattc
     5461 ggcgacaaac tggctgctgc cagcgtcaac gtcaacaacg gcttcggcaa cctgcttgcc
     5521 aatctggaca aactggatac ggacacccgc gccgccgtcg aagccgaaat cgccgccgtt
     5581 tacgccgcca accccgattt ggcgatggtc gattccgaca aaggcatcac caacctgcac
     5641 gttcccagcg atgtcatcgt tgatgcctct atgcctgcga tgattcgcaa ttccggccgt
     5701 atgtgggaca aagacggcaa agcgcaagac accaaagccg tgattcccga cagcagctat
     5761 gccggcgttt atcaggcaac catcgacttc tgccgcgaac acggcgcatt cgatccgaca
     5821 acaatgggta cagtacccaa tgtcgggctg atggcgcagg cagccgaaga atacggttcg
     5881 cacaacaaaa cttttgaaat cgaagccgac ggccgggttc aagtggttga tgaagcagga
     5941 aatgtcctga tgcagcacga cgttgaagca ggcgacatct ggcgtatgtg ccaaaccaaa
     6001 gacgctccag ttaaagactg ggtacaactc gccgtcaacc gtgcccgcct gagcaatacg
     6061 cccgccgtat tctggctcga cgaaaaccgt ccgcacgaca agagcctgct cgccaaggtt
     6121 aaagcctacc ttgccgaacc ggataccgac ggcctcgata tccgcgtcct cgctcccgaa
     6181 gaagccgcca agttcagcct gggccgtctg aaaaacggcg aagacaccat ctccgtaacc
     6241 ggcaacgtcc tgcgcgacta cctgaccgac ctgttcccga ttttggaact gggtaccagc
     6301 gcgaaaatgc tgtctatcgt tccattgatg aatggcggcg gtatgtttga aaccggcgcg
     6361 ggcggttctg caccgaaaca cgttcaacaa ttcctcgaag aaaaccactt gcgttgggac
     6421 tctttgggcg aattcctcgc gctcgccgta tcgtttgaac atctggcgca aaaaaccggc
     6481 aacgccaaag cccaagtcct cgccgacact ttggatgccg ccaccgaaaa actgctgcta
     6541 aacgacaaat cgcccaaacg caaagccggc gaactcgaca accgcggcag ccatttctac
     6601 ctcaccctct actgggcgca agaattggcg gcgcaagaca aagatgccga actgaaagcc
     6661 gcatttgcgc cgttagccaa atcgttggcg gaaaacgaag ccaaaatcgt tgaagaactc
     6721 tctgccgtac aaggcaaagc ggtcgacatc ggcggctact acgcagccaa tcctgaaaaa
     6781 gcggcacaag cgatgcgccc aagcgcaacc tttaatcagg cactaaacgc cttataagca
     6841 ccaaggcaaa aatgctgtct gaacatttgt cgttcagacg gcattagtta tccctatctg
     6901 tctgattatg attaaatgcc gcacccattg gatat
//
LOCUS       DAWFJW010000104         6912 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFJW010000104 DAWFJW010000000
VERSION     DAWFJW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.104"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            229..2358
                     /locus_tag="WGR19_001701"
                     /pseudo
     CDS             229..2358
                     /locus_tag="WGR19_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2600..4066
                     /locus_tag="WGR19_001702"
     CDS             2600..4066
                     /locus_tag="WGR19_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0799193.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4267..5235)
                     /locus_tag="WGR19_001703"
     CDS             complement(4267..5235)
                     /locus_tag="WGR19_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799194.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGTDDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5313..6605
                     /locus_tag="WGR19_001704"
     CDS             5313..6605
                     /locus_tag="WGR19_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0799195.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
BASE COUNT         1636 a         1753 c         2063 g         1460 t
ORIGIN      
        1 tttctcgttt cgggtgattt ctaaaccgtc attcccgcgc aggcgggaat ccagaccttc
       61 aaaccccggc catccccgat aaattcttgc agcattgaaa ttctagattc ccactttcgt
      121 gggaatgacg gcggaggggt tttggttttt tccgatttct cattgcgatg tagtgtgatg
      181 tagtatataa tcattataat tttaacactt gacaaaggaa aatttctcat gacactgaaa
      241 gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa
      301 gcaagcaagc aagcaagcgg tcgggttaat ctattaacat tatctgtttt atcgctgttt
      361 tgcacgccat atgtttgagg ttcggatgcg tacgatcccg tcaaagaagc cgagattaaa
      421 aacaaattta ttttagaagc ggcggaagac agaaattccc acgtttggcg cggcccgtgc
      481 agcatatctt ttgattgctt cggtgctttc agttatcagc ccggttcgga tactcgttct
      541 acaaatattg gcgatgctgc cgatttttca ttttcagaca agccggtaaa cggtgtttcc
      601 cattattttt ccagcggtaa aaccgatcaa aattcatccg aatatgggta tgacgaaatc
      661 aatattacgg gtaaaaacta caatagcggc atcctcgccg tcgataatat gcccgttgtt
      721 aagaaatatg ttacagagaa ttacggaact gatttaaagc aggcggttaa gaagcaatta
      781 aaggctttgt acgacacgaa acccgaagat tgggaaaaaa ataaaaaaat agttgaggaa
      841 gtgtatagaa aacagcttgg aggaacattt gatatactta aacagaaaag acccaatata
      901 attaatcaat tgatagaaga cgccgtactt actccttata caccacagac taatctcgac
      961 aacatcttca ataaaacact gcacgtcaaa atcgaaaaca aatcccacgt cgccggacag
     1021 gtgttggaac tgaccaagat gacgctgaaa gattcccttt gggaaccgcg ccgccattcc
     1081 gacatccata tgctggaaac ttccgataat gcccgcatcc gcctgaacac gaaagatgaa
     1141 aaactgaccg tccataaagc gtatcagggc ggtgcggatt tcctgttcgg ctacgacgtg
     1201 cgggagtcgg acaaacccgc cctgaccttt gaagaaaaag tcagcggaca atccggcgtg
     1261 gttttggaac gccggccgga aaatctgaaa acgctcgacg ggcgcaaact gattgcggcg
     1321 gaaaaggcag actctaattc gtttgcgttt aaacaaaatt accggcaggg actgtacgaa
     1381 ttattgctca agcaatgcga aggcggattt tgcttgggcg tgcagcgttt ggctatcccc
     1441 gaggcggaag cggttttata tgcccaacag gcttatgcgg caaatacttt gtttgggctg
     1501 cgtgccgccg acaggggcga cgacgtgtat gccgccgatc cgtcccgtca aaaattgtgg
     1561 ctgcgcttca tcggcggtcg gtcgcatcaa aatatacggg gcggcgcggc tgcggacggg
     1621 cggcgcaaag gcgtgcaaat cggcggcgag gtgtttgtac ggcaaaatga aggcagccga
     1681 ctggcaatcg gcgtgatggg cggcagggcc ggccagcacg catcagtcaa cggcaaaggc
     1741 ggtgcggcag gcagttattt gcatggttat ggcgggggtg tttatgctgc gtggcatcag
     1801 ttgcgcgata aacaaacggg tgcgtatttg gacggctggt tgcaatacca acgtttcaaa
     1861 catcgcatca atgatgaaaa ccgtgcggaa cgctacaaaa ccaaaggttg gacggcttct
     1921 gtcgaaggcg gctacaacgc gcttgtggcg gaaggcgttg tcggaaaagg caataatgtg
     1981 cggttttacc tgcaaccgca ggctcagttt acctacttgg gcgtaaacgg cggctttacc
     2041 gacagcgagg ggacggcggt cggactgctc ggcagcggtc agtggcaaag ccgcgccggc
     2101 attcgggcaa aaacccgttt tgctttgcgt aatggtgtca atcttcagcc ttttgccgct
     2161 tttaatgttt tgcacaggtc aaaatctttc ggcgtggaaa tggacggcga aaaacagacg
     2221 ctggcaggca ggacggcgct cgaagggcgg ttcggcattg aagccggttg gaaaggccat
     2281 atgtccgcac gcatcggata cggcaaaagg acggacggcg acaaagaagc cgcattgtcg
     2341 ctcaaatggc tgttttgatg cgtcgggaaa tgttttgacg cacaggcggc acaccggcac
     2401 ggccccgcgc gccgccccgc aaaccaatcc gaaccctgcc gccccgaagg gcggggcatc
     2461 atgatgaaac cggcggaaaa ccgccggttt tttgccgccg tttgaaaccc gattctggct
     2521 tcagacggca ttgtcgcggc atcgggcggc agggtttgga acagcggcat aaaaaactga
     2581 tacaatccgc cgattgataa tggttatttt ttatttttgt gggaagacat ttatgcctgc
     2641 acgaaacaga tggatgctgc tgctgccttt attggcaagc gcggcatatg ccgaagaaac
     2701 accgcgcgaa ccggatttga gaagccgtcc cgagttcagg cttcatgaag cggaggtcaa
     2761 accaatcgac agggagaagg tgccggggca ggtgcgggaa aaaggaaaag ttttgcagat
     2821 tgacggagaa accctgctga aaaatcccga attgttgtcc cgcgcgatgt attccgcagt
     2881 ggtctcaaac aatattgccg gtatccgcgt tattttgccg atttacctac aacaggcgca
     2941 gcaggataag atgttggcac tttatgcaca agggattttg gcgcaggcag acggtagggt
     3001 gaaggaggcg atttcccatt accgggaatt gattgccgcc caacccgacg cgcccgccgt
     3061 ccgtatgcgt ttggcggcag cattgtttga aaacaggcag aacgaggcgg cggcagacca
     3121 gttcgaccgc ctgaaggcgg aaaacctgcc gccgcagctg atggagcagg tcgagctgta
     3181 ccgcaaggca ttgcgcgaac gcgatgcgtg gaaggtaaat ggcggcttca gcgtcacccg
     3241 cgaacacaat atcaaccaag ccccgaaacg gcagcagtac ggcaaatgga ctttcccgaa
     3301 acaggtggac ggcacggcgg tcaattaccg gctcggcgcg gagaaaaaat ggtcgctgaa
     3361 aaacggctgg tacacgacgg cgggcggcga cgtgtccggc agggtttatc cggggaataa
     3421 gaaattcaac gatatgacgg caggcgtttc cggcggcatc ggttttgccg accggcgcaa
     3481 agatgccggg ctggcagtgt tccacgaacg ccgcacctac ggcaacgacg cttattctta
     3541 caccaacggc gcacgccttt atttcaaccg ttggcaaacc ccgaaatggc aaacgttgtc
     3601 ttcggcggag tgggggcgtt tgaagaatac gcgccgggcg cgttccgaca atacccattt
     3661 gcaaatttcc aattcgctgg tgttttaccg gaatgcgcgc caatattgga tgggcggttt
     3721 ggatttttac cgcgagcgca accccgccga ccggggcgac aatttcaacc gttacggcct
     3781 gcgctttgcc tgggggcagg aatggggcgg cagcggcctg tcttcgctgt tgcgcctcgg
     3841 cgcggcgaaa cggcattatg aaaaacccgg ctttttcagc ggttttaaag gggaaaggcg
     3901 cagggataaa gaattgaaca catccttgag cctttggcac cgggcattgc atttcaaagg
     3961 catcacgccg cgcctgacgt tgtcgcaccg cgaaacgcgg agtaacgatg tgttcaacga
     4021 atacgagaaa aatcgggcgt ttgtcgagtt taataaaacg ttctgattgc tgttcctttt
     4081 cggaggaaac cctgccggcg gcggtatcac ggcgggcatc ggcggctttc gggcggtgct
     4141 ttgcgtgccg ccgcgtgtgc ggaaacgcat tccggttttt ccggcataac ggcgatgcga
     4201 ggtaaaatgc cgtctgaaac ccgattcggg cttcagacgg cattgtcgcg gttgcggcag
     4261 gcgggttcac cagattccgt caaaggtttt cgcgccgcgc caaaatttcc acctgtcggc
     4321 gggtttgaag gtcagcgtac cgccgtgttg tccgtccgtg gtgatgtcca gccgtttgat
     4381 tttgccggta cggacggctt cgtagatcgg tgcgaaccag tgttcttccc actgctgtaa
     4441 aaccgatgca taccgctccc tgtcccctgt cagggcggtc aggcgcaaat cgtccataaa
     4501 caggatatgg tgcgtgtcgg gcaggtgtgc cgccgtttct tcataggcgc ggaagttgtc
     4561 gggcaatgcg cggcggtcgg ggtggaaacg gctccaaacc gtatcggcga aaagcgtgcc
     4621 gccttgactg cttccgtgca gactgtccca aagccataag ccgttcaact cgggcagccc
     4681 gcgttttttg cggttatggt tgacggggtg tgccgccagc cacatttgga tttcggtttg
     4741 gacgcgcagc cattccaacg catcttctcc gtccggctga tcgtccgtgc ccaacaatcc
     4801 gcccaagtcc aaaacgggct tcgcgcccca gcggtacgcg cggggaaggg aaaccagcca
     4861 taattcaggc aggacgggaa cgaaacgcca cggaatatcg ccgtaaaacg ccgacaggtc
     4921 gcggcagagc cgttccgctt catccgtacc gacgttcaga tattccgccg tcagcacatt
     4981 tgcctgatgc atccccatct tttgccagac gggcgtggcg agcgcgacgg cttcagacgg
     5041 catattcagg ctttgcgccg cgcgttccgc cagtctgccg caccacaaat aacgcgcgta
     5101 aaatgccgaa gccgtgcagc tttggcggtg cagcgagccg tattgcagga ttttgttgaa
     5161 ggcgggcagg cagagaggta ttcgggtttc gtcttcatcc aaattgagcg agggaatggc
     5221 gagggtgagt ttcatcgttt gacgtttcag aaatgcaggt caggcgcaac attatagagg
     5281 attcggcgca aacgccgtca aaaaggaaca atatggctgt cttcccactt tcggcaaaac
     5341 atcggaaata cgcgctgcgc gcgcttgccg tttcgattat tttggtgtcg gcggcataca
     5401 ttgcttcgac agagaggacg gagcgcgtca gaccgcagcg cgtggaacaa aaactgccgc
     5461 cgctgtcttg gggcggcagc ggtgttcaga cggcatattg ggtgcaggag gcggtgcagc
     5521 caggcgactc gctggcggac gtgctggcgc gttcgggtat ggcgcgggac gaaattgccc
     5581 gaataacgga aaaatatggc ggcgaagccg atttgcggca tttgcgtgcc gaccagtcgg
     5641 ttcatgtttt ggtcggcggc gacggcggcg cgcgcgaagt gcagtttttt accgacgaag
     5701 acggcgagcg caatctggtc gctttggaaa agaaaggcgg catatggcgg cggtcggctt
     5761 ctgaggcgga tatgaaggtt ttgccgacgc tgcgttcggt cgtggtcaaa acgtcggcgc
     5821 gcggttcgct ggcgcgggcg gaagtgcccg tcgaaatccg cgaatcctta agcgggattt
     5881 tcgccggccg cttcagcctt gacggtttga aggaaggcga tgccgtgcgc ctgatgtacg
     5941 acagcctgta tttccacggg cagcaggtgg cggcgggcga tattttggcg gctgaagtcg
     6001 ttaagggcgg cacaaggcat caggcgttct attaccgttc ggacaaggaa ggaggagggg
     6061 gcggcaatta ttacgatgaa gacggcaggg tgttgcagga aaaaggcggc ttcaacatcg
     6121 agccgctggt ctatacgcgc atttcttcgc cgttcggcta ccgtatgcac cccatcctgc
     6181 acacttggcg gctgcacacg ggcatcgatt atgccgcacc gcagggaacg ccggtcaggg
     6241 cttccgccga cggcgtgatt acctttaaag gccggaaggg cggatacggc aacgcggtga
     6301 tgatacgcca cgccaacggt gtggaaacgc tgtacgcgca cttgagcgcg ttttcgcagg
     6361 cacaaggcaa tgtgcgcggc ggcgaggtca tcggttttgt cggttcgacc gggcgttcga
     6421 ccgggccgca cctgcattac gaggcgcgca tcaacgggca gcccgtcaat cctgtttcgg
     6481 tcgcattgcc gacaccggaa ttgacgcagg cggacaaggc ggcgtttgcc gcgcagaaac
     6541 agaaggcgga cgcgctgctt gcgcgcttgc gcggcatacc ggttaccgtg tcgcaatcgg
     6601 attgaagttt gaaccggcga cgaaaacaat gccgtctgaa aatctgcaaa ccggttttca
     6661 gacggcattt gcagttgatt aaaataaaat taagccaagg aagcactgcc gtcattcctg
     6721 taccggcggg aatccggaca ccacggcacg gaaacccatc cgccgtcatt cccgcgaaag
     6781 cgggaatcta gaaatacaac gcggcaggag tttatcggaa atgacggaaa cccaacgcac
     6841 cggattcccg ctttcgcggg aatgacgaag tgggtgggaa tccggattta tccgttccgg
     6901 cagtgtttgc aa
//
LOCUS       DAWFJW010000105         6602 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFJW010000105 DAWFJW010000000
VERSION     DAWFJW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.105"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(1..153)
                     /locus_tag="WGR19_001705"
                     /pseudo
     CDS             complement(1..153)
                     /locus_tag="WGR19_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            complement(1363..2301)
                     /locus_tag="WGR19_001706"
     CDS             complement(1363..2301)
                     /locus_tag="WGR19_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0799196.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYNPLALDLDGDGIETVATKGFAGALFDHRNQG
                     IRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNG
                     DNIINAADAAFQSLRVWQDLNQDGISQTNELRTLEELGIQSLDFAYKDVNKNLGKESK
                     PQSAVLAIHTPFVCFLGNLKGIICTRHNPILYFTKN"
     gene            complement(2519..3238)
                     /locus_tag="WGR19_001707"
     CDS             complement(2519..3238)
                     /locus_tag="WGR19_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799197.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            complement(3514..3948)
                     /locus_tag="WGR19_001708"
     CDS             complement(3514..3948)
                     /locus_tag="WGR19_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799198.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4285..4974
                     /locus_tag="WGR19_001709"
     CDS             4285..4974
                     /locus_tag="WGR19_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0799199.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRMVISHYLALAANEVFEGAVELDESYFGGRRKGKRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSDTLMPVIKQKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDCKSFPLFLKECEFRFNFGTPSRQLKILRDWCGFR
                     ANLVQPRFFYL"
     gene            complement(5106..5621)
                     /locus_tag="WGR19_001710"
     CDS             complement(5106..5621)
                     /locus_tag="WGR19_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799200.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGVGVFGAGGKYSMHIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNVRIQ"
     gene            complement(5882..6439)
                     /locus_tag="WGR19_001711"
     CDS             complement(5882..6439)
                     /locus_tag="WGR19_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HEZ0799201.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARAAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAQQGSYTKKDGTTAQAGDLLLAADNLHSRLTDKMLSISHV
                     RENTISPFVLGCLKQITINVSHYLK"
BASE COUNT         2193 a         1221 c         1022 g         2166 t
ORIGIN      
        1 ggcaacggtt tctatgccgt ctccgtccaa atctagggca agggggtcgt aaacgtggta
       61 tttgcctgaa cgatttctgt ctaattcttt ccgtagattc caatcatcag gtagccagtc
      121 atatacccta tgagaccagt cttcaaattt ctccttcagg agactgccaa tgctttcaga
      181 atcaaaagaa atatttctat ctgttaaatt atgaaatgaa ttctgaatct ccttaaattt
      241 attcctgata ttatttaatg tttcttttaa tgaattttct aaagaatcat taaatgtacc
      301 aaggtttttt ccccaatcat atccattttc tatatttgga acattaggcg ggttgttata
      361 gtatttcccc tcagtattga tattactatt agataacgaa ttccttaaat ttaccgctcc
      421 acctccagca atgcaaatat ccttagaaac accaattgca ccagcacaag ctactccaac
      481 atcaaaagat gcaatgggct gaaacgctgg cacaaatccc ccatacgatt ttccatggaa
      541 tgaacgctgc aaatcttttt ctttacctgt accatagttt agtagattaa gtggattctc
      601 cgctccataa gcccatgctt catgagggtt gtatccgacg ggagcaatat aaggattacc
      661 attcttatct tttagataac caccagctcc atctgaaaca tacgaagctt taactgtacc
      721 aataatattt tgcttaggat cttttggatt atattttgta tatacattta cctcaaaaaa
      781 tttccataac tgagccttta cttgttgata aaagataaaa taaaattaga aactaaaagt
      841 gagaaaaagt taataataat agggatgtat aaatgtaaag gttctgtttc atagctaagg
      901 ttatctgaat atatggaaaa aaagtaaaag tccataaaag taaaatatat agataatgat
      961 aatgataata gaattatcac tttcctagag taaggataat atttttttac gataaaaaaa
     1021 tataaaatag gataaataat tgccatatag atagattttg tagcaaaatt aaaaataaat
     1081 aataaaaata atgtaataaa gtatattttt tggctatgaa ataaaattgt acataattga
     1141 acgagcagat caaaaaatga actacatata acaataaata ataacgtatt taccatacta
     1201 aatttaatag gtctcattat catatttaat aaccacttca tagtaaatgc agagtgggtg
     1261 gaaacactca ctttatggtt tgctacgctc tgctcaatta gcaacccgat aacccaatat
     1321 agataatagg gcaattaatc caatctaatt tgttaaaata cgttaatttt ttgtaaaata
     1381 aagtattgga ttatgtcggg tgcaaatgat gccttttaga ttgcctaaaa aacaaacaaa
     1441 cggtgtgtgt atagcaagta ctgcactttg cggcttactt tctttaccga gatttttatt
     1501 tacatcttta taggcgaaat ccaaagattg aatacccaat tcttcaaggg tgcggagttc
     1561 gttggtttgg gaaatgccgt cctgattgag atcctgccat acacgcaggg attggaatgc
     1621 ggcgtctgcc gcgttgatga tgttgtcgcc gtttgaatcc aattcggcca aagccgcgta
     1681 gccgtgtttg gcaaaagaac cgtctgccag tttggtgttg tcgccgaaga gttccgcacc
     1741 gttgtcgatg atgccgttgc cgttcaaatc gcggacgagt aaaccgtcat cggcagaaac
     1801 ccaaccggtg gcggtgcgga tgccttgatt gcggtggtcg aacaatgcac ctgcaaagcc
     1861 tttggtggca acggtttcta tgccgtctcc gtctaggtct agggcgaggg ggttgtagat
     1921 gtggtattgg cctgaacggt ttctgtccaa ttctttccac agattccagc cgtcgggcag
     1981 ccagtcgtat acccaacgag accagtcttc aaatagctca tacccaaaac ctttccagtt
     2041 gtcaaaatta taaaattcct ctgtagatat cgtaccagta tctcctgtgg caatagccgt
     2101 tgctgcaatt atatctccag caattgctaa tggaatcccc caactctgca tatttccatt
     2161 cttctctatt aatgtacttc ctaaccctcc caatgaatat aaatccgcta aaatattttg
     2221 aaaacggttg atattaactt taccatcttt atgctcctga atagctgaag atatatttcc
     2281 agagagacgg cttgcaatca cagaggcatt tgttccgttt acaatatgcg atgacgtact
     2341 acctggtgca atattactag ccaaattacc aaatgctaat aatgcactat aaccagcttg
     2401 tttgtaatat tcattacgtt tcgagatatc tttctcattt tttgcagaaa ctaaattagc
     2461 aaccatattt ccaaattctg ctaacgcgga tgagcgtgcg ctcataaaat taccaaaact
     2521 attccaactc attgaagtaa gtcctatata aaagcaaatt aaattttaaa tataaaataa
     2581 ttaatacgat acaaacactg ttttcaataa gaaaaaatgt tttatactta tctaacaagt
     2641 acaaactttt gtttccatca ttaatttcaa atattacatt atctataaaa tctccttgag
     2701 gttgattata aaaataaatt ttaacaggtt tttttgcaaa ttttataaaa ttggcacatg
     2761 gagcatatga atgaatacca caaaccaagc ttaatcttat ggatttattt ttaacatcag
     2821 atgcaactac atcaatataa ggtgtttctc taattaccct accatatcta tgattatctt
     2881 ttaatttagc atatccattt gagtcataaa cataattata actatcatgt ggttttatca
     2941 ccccagtaat cgaggatata ttttgttcct ttatttgatg ctcaaattta taaaatttta
     3001 tatttatcat actaatatga attaccatcc atataataat agaaatcggc aataaaaaca
     3061 ataatttgtt ttttatgttt ttttctaaaa ataataaatt tacagcaaga aagacgtttg
     3121 caaaaaacaa aaacgcaaaa aaatcaaata gaaaaacaaa ataaccaaac actatattaa
     3181 ttactatcaa ggctatatgt aaaactataa atactagtaa ataattttta tttttcatat
     3241 gtctcattaa ataaaatata gctggtatga tttagtgaat atcaatttag tggcatagag
     3301 tgtatactca tcgtattcac tctctcagtc ttcaggctgc ctgaaaagcc tttggcggtg
     3361 actgtttcta tgccgtcgcc gtccaagtct agggcgaggg gatcgtagac gtggtatttt
     3421 ccatcacacg cagaaaattt tttgcattgt ttagtatcac cactaccaat cattgttact
     3481 ctccttgagt ttaagtcaaa caaaaaatga aattcaaata ctctctctta acttattgag
     3541 atcaatcatc aatataaata aaaaatataa actaccagaa taaccaaagc cacgaaaaat
     3601 tttttccaat atttcactat ttaaacaaaa agcaccgcat acccacgttg atgattccga
     3661 aataaaacac atccatattt ccattactgc caaccatgac actactatcc taatcaaaaa
     3721 tactttttta tctgcccaag cactttttat atttaacaat acaatatgat tttgtaattt
     3781 aaaatctaag ctttgcattt ttctaaccag tgcaaacaaa caaccaagcg caataaaaaa
     3841 agattggtag gactggtaaa aaggtagaaa tagaatattt aagaccaatg gatagatcca
     3901 catataccta tttgttaaat tattaataaa ttcttttttg ttatccataa aatttccata
     3961 aaatattgcg ctgatattgc actgagttta ttagcaaaca acgtaaagaa aatatttatt
     4021 atgataagtg ttttttaagt ttcattttca aatgatggcg cgtaataaaa agtttcacaa
     4081 atccgttttt cagactatct aagtatctat tttacaatct atttcaataa tcgttatgtc
     4141 ttaacaactc ttcttggcat tatagatgct cactcatatc atttaatgat actcttgatt
     4201 aatctttatg cgatttagcg aaattaaaat ttctcttttt tgtttgaggc tgtactagat
     4261 tagcagatat gttacccccg aaatatgaag ataacacact gcaaattaaa gaaaaaagta
     4321 cagaaagagc tgctccgttt ttttgtactc gaagttaccg cccgttctgc tgccgatatt
     4381 ttgggtatcc atcccaattc ggcagtcctg ttctaccgca aaatccgtat ggtcatcagc
     4441 cattatctgg ccttggctgc caatgaggtt ttcgagggcg ctgtcgagtt ggacgaaagc
     4501 tatttcggcg gacggcgtaa aggcaaacgt ggtcgcggtg cggcaggaaa agtggttgtc
     4561 ttcggcattc tgaaacgtaa cggacgggtc tataccgttg tcgtagataa tgccaagtcc
     4621 gatactttaa tgcctgttat caaacagaaa atcatgccgg acagtattgt ttataccgat
     4681 agtctgagca gctgcgacaa gttggacgtg agcggtttta tccattaccg catcaaccat
     4741 tccaaggaat ttgcagaccg tcagaaccac attaacggca ttgagaattt ttggaatcag
     4801 gcaaaacgcg tcttgcgaaa atacaacgga atcgattgca aatctttccc actgttcttg
     4861 aaagaatgcg aatttcgatt taacttcggc acaccgtccc gacagctaaa aatcttgcgg
     4921 gattggtgtg gatttagggc taatctagta cagccccgat ttttttatct ctagccgaat
     4981 acagttcaac ccaaacgtac ccgtcttgga tacaccccct tgtttcagtg gcgtaccctg
     5041 ctccgcaaat tttacagtta tcgcataata atcagccata agtaacccca catttctaga
     5101 aaaatttact gaattcgtac attaactact attcttatag aattatttat ttcatcgtaa
     5161 agtggaatat attcactatt atcagaaatc tcaaataaat attccccttc cggcaaataa
     5221 aaatcataaa tatgcatact gtattttccc ccagctccaa atactccgac ccctaatttt
     5281 ctacttctct ctgtaagcaa ctcatctata ataagttttt taccatcttc ttccacttta
     5341 aatattttaa ccgatatatt atatggatag tttaacttat catcaatcca cctctccccc
     5401 tgttttggat cataatgtat aaaattagtt cctaacgata ttgcataagg tttaccaatt
     5461 ttctgtttga gttcaataga tctttttgtt tttttaccaa gaggaaagtc taccacaaca
     5521 ggttctgggc taatagccat ataaaaaata gtttcaacaa aactgcagct agcaatcaat
     5581 aggcaagata ttattaataa tatttttcta aatgcagaca taataaaaat ttccaaaaaa
     5641 tccatatcag aatatactaa ttatttgtat aattaataag gaagagaaag actcaaaacg
     5701 gctatgagat ttccgcctga aaataatgaa ataaaagtcc atagtaatga ataatagctt
     5761 tatcagcccc ccctttaatg caaacataga gaatacttcc cttgaagcac tatcaatttc
     5821 tacttaacta aaactctagc tctattttaa aattttgtca aaaatagtta aatactttta
     5881 tttattttaa ataatgagat acatttatgg ttatttgttt tagacagcct aaaacaaacg
     5941 gggaaatcgt gttttcccga acatggctaa tggatagcat tttgtccgtg aggcggctgt
     6001 gcaggttgtc agcagccaat agcaaatcgc ctgcttgtgc ggttgtgccg tcttttttgg
     6061 tgtagctgcc ttgttgagcc aaagtgttgc cgttaccaag atttttgttt acatcttttc
     6121 gagaaaattg gctttgcatt tgccttaaca aagatatttc tgctgctcgt gctttattat
     6181 ttatattata tttactctgc atttttctta caacttgtct caactgactt tgtgtttttt
     6241 gtaattcacg ttgtaccgat tttcctaaac tttcatgttt ggctgcataa ttggtagaat
     6301 cagttgacat ttgtgtataa aaatttttac gaaaattttc gtaactcaga ttaattacat
     6361 ccgtacttgc agttataaaa tcttgtataa attcaataga aactgaatta tcatcagatt
     6421 tggctttgtt attttccata ttgagcctct cttaatttga aaaaattaac ataaacattt
     6481 aaaaataaaa caaaattata taataaaaaa aataatgaga atataaagga taaaaagaga
     6541 gttaagttat ttaatcggac ttgttctcta taaatcataa ggcaccaaat aagccataat
     6601 aa
//
LOCUS       DAWFJW010000106         6545 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFJW010000106 DAWFJW010000000
VERSION     DAWFJW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.106"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            97..1170
                     /gene="hupA"
                     /locus_tag="WGR19_001712"
     CDS             97..1170
                     /gene="hupA"
                     /locus_tag="WGR19_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
                     /protein_id="HEZ0799202.1"
                     /translation="MKYKALSLLPLAAALAACAGGGVAEPHVPVSIPTATPLTGEVKL
                     SSDNSKIENINTANTETHPPRRTRRSLYASPQNTSSGISIQQREVEKIYFGVKSPEKS
                     FIFQTPGGAQYALSSYADPIVPSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSY
                     AEITKQDYMKFGAWIGPNGEIDLFAGGFPIGKTPPPAFSWGSPTTETALSKGKITYQV
                     WGIRVRNGQFVTSSYTPPKGSSWSGYQNTPVLSFITANFNSNKLAGEIIGNSDYGPNV
                     KIENATIDGLSFSGDATSNGKTGNLEGKFFGKFNSTRSSDTGIGGKITFKDDRSLDTV
                     FGGVIYEKKLDDKTSQDTNHLKK"
     gene            1201..3633
                     /gene="hupB"
                     /locus_tag="WGR19_001713"
     CDS             1201..3633
                     /gene="hupB"
                     /locus_tag="WGR19_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0799203.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSP
                     VWQNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4221..5855)
                     /gene="groL"
                     /locus_tag="WGR19_001714"
     CDS             complement(4221..5855)
                     /gene="groL"
                     /locus_tag="WGR19_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0799204.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(5948..6238)
                     /locus_tag="WGR19_001715"
     CDS             complement(5948..6238)
                     /locus_tag="WGR19_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0799205.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            6467..>6545
                     /locus_tag="WGR19_001716"
     CDS             6467..>6545
                     /locus_tag="WGR19_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0799206.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTA"
BASE COUNT         1613 a         1928 c         1570 g         1434 t
ORIGIN      
        1 gatttttgca ccgacccaca aaaccagttg acatcatata acatttaaac tgataatcaa
       61 aactattatt aacacatcat caagattgag tatgcgatga aatacaaagc cctctcctta
      121 ctgccgcttg ctgccgccct tgccgcctgt gccggggggg gggtagccga accgcacgtc
      181 cccgtgtcca tccccaccgc cacgccgctg accggtgagg taaagttatc aagcgataac
      241 agcaaaatcg aaaacatcaa caccgccaac actgaaacac atccgccgcg ccgcaccaga
      301 cgctccctct atgcctcccc gcaaaacaca tcctccggta tttccattca gcagcgggaa
      361 gtagaaaaaa tctatttcgg tgttaaatca ccagaaaaat cattcatctt ccaaacaccc
      421 ggcggcgcgc aatacgccct ttcatcctac gccgacccca tcgtccccag ctactcctcc
      481 cccgacttca aaattcccga ccgccacgca gggcagcggc ttgccgacgg cagccgcatc
      541 tttatctgct gcagcgactc cggggctact tcgtatgcgg aaattaccaa acaagattat
      601 atgaaattcg gggcgtggat aggccccaac ggcgaaatcg acctcttcgc cggcggcttc
      661 cccatcggca aaacgccgcc tcctgcgttc tcctggggca gccccacgac cgaaaccgca
      721 ttgtcaaaag gcaaaatcac ttatcaggtt tggggcatcc gcgtcagaaa cgggcaattt
      781 gttacttcct cttatacgcc gcccaaaggc agctcttgga gtggttatca aaacaccccc
      841 gtcctttcct ttatcaccgc caatttcaac agcaacaaac tggcaggcga aatcatcggc
      901 aacagcgatt acgggccaaa tgtgaaaatt gaaaacgcaa cgattgacgg actatccttt
      961 tccggcgatg ccacctccaa cggcaaaacc ggcaacttgg aaggcaagtt ttttggcaag
     1021 ttcaacagca cccgcagcag cgacaccggc ataggcggca aaatcacttt taaagacgac
     1081 cgctccctcg ataccgtatt cggcggcgtg atttatgaga aaaaacttga tgacaaaacc
     1141 agtcaagata ccaaccatct caaaaaataa taacccatcc gcttttaaaa aggttttctt
     1201 atgcccatcc cctttaaacc cgtattggct gccgccgcca tcgcccaagc gtttcccgcc
     1261 tttgcggcag accccgcgcc gcagtccgcc caaacgctga acgaaatcac cgttaccggc
     1321 acgcacaaaa cccaaaaact cggcgaagaa aaaatccgcc gcaaaacttt agacaagctc
     1381 ttggtcaacg acgaacacga cctggtgcgc tacgaccccg gcatttccgt cgtcgaaggc
     1441 ggcagggcgg gttctaacgg ctttaccata cgcggcgtgg acaaagaccg cgtcgccatc
     1501 aacgttgacg ggctggcgca ggcggaaagc cgctcttccg aagccttcca agaattgttc
     1561 ggcgcgtacg gcaacttcaa cgccaaccgc aacacttccg agccggaaaa cttttccgaa
     1621 gtaaccatca ccaaaggcgc ggactcgctc aaatccggca gcggcgcatt gggcggcgca
     1681 gtcaattacc aaaccaaatc cgcaagcgat tatgtttccg aagacaagcc ctaccatttg
     1741 gggataaagg gcggcagcgt cggcaaaaac agccaaaaat tcagcagcat caccgccgcc
     1801 ggcaggctct ttggtttgga tgccttattg gtttataccc gccgcttcgg caaagaaacc
     1861 aaaaaccgct cgaccgaggg cgatatcgaa attaaaaacg acggatatgt ctataacccg
     1921 accgatacag gcggacccag caagtacctg acctatgtag ccacaggggt tgcgcgctcc
     1981 caacccgacc cgcaagaatg ggtaaacaaa agcaccctgt tcaagctggg ctacaacttc
     2041 aacgatcaaa accgtatcgg ctggattttt gaagactcgc gcaccgaccg ttttaccaac
     2101 gagctgtcta atttgtggac gggtacgacc acgtctgccg caacgggcga ctaccgccac
     2161 cgccaagacg tgagctaccg ccgccgctcc ggtgtcgaat acaaaaacga attggaacac
     2221 ggcccgtggg acagcctcaa gctgcgctac gacaagcagc gcatcgatat gaacacttgg
     2281 acttgggaca tcccgaaaaa ttacgatttg cgcggcatca acggcgaggt ttaccattcg
     2341 ttccggcata tccgccaaaa caccgcgcaa tggactgccg attttgaaaa acaactcgac
     2401 ttttccaaag ccgtttgggc ggcgcaatac ggcttgggcg gcggcagagg ggacaatgcc
     2461 aactcggatt acagttattt cgcaaaactg tacgccccca aaatcctcgc ttccaaccaa
     2521 gccaaaatca cgatgctgat cgaaaaccgg tcgaaatata aatttgccta ttggaacaat
     2581 gcgtttcact tgggcggcaa cgaccgcttc cgcctgaatg cgggcatacg ctacgacaaa
     2641 aacagcagca gcgcgaaaga cgatccgaaa tacaccaccg ccatccgagg gcagattccc
     2701 catttgggtt cggaacgcgc gcacgccggc ttcagctacg gcacggggtt cgactggcgg
     2761 tttaccaagc atctgcactt attggcaaaa tacagcaccg gcttccgcgc accgacttcg
     2821 gacgaaacct ggctgctgtt cccacacccc gatttctacc tgaaagccaa cccaaacctg
     2881 aaagccgaaa aagccaaaaa ctgggaattg ggtctggcgg gcagcggcaa agcgggcaac
     2941 ttcaagctct caggcttcaa aaccaaatac cgcgacttta tcgaactgac ctacatgggc
     3001 gtttcgtcag acgataaaaa caaccccaga tacgccccgc tttcagacgg tacggcattg
     3061 gtcagctcgc ccgtttggca aaaccaaaac cgctccgccg cctgggtgaa aggcatagag
     3121 tttaacggca cgtggaacct cgacagtatc ggtttgccca aagggctgca caccggcctc
     3181 aacgtcagct acatcaaggg caaggcaacg caaaacaacg gcaaagaaac gcccatcaac
     3241 gcgctttcgc cgtggacggc ggtttacagc ctgggctatg acgcgccttc caaacgctgg
     3301 ggcatcaacg cctacgccac gcgcaccgcc gccaaaaagc cgtccgacac cgtccacagc
     3361 aacgacgact tgaacaaccc gtggccttat gccaaacaca gcaaggccta tacgctgttc
     3421 gacctttccg cctacctcaa catcggcaaa caggtaacgc tccgcgccgc cgcgtacaac
     3481 attaccaaca agcagtacta cacttgggaa tctttacgca gcatccgcga gttcggcacg
     3541 gtcaaccgcg ttgacaacaa aacccacgcc ggcatccaac gctttacctc gccgggcagg
     3601 agctacaatt tcaccatcga agcgaagttc taagctcctg atgtgccaat gccgtctgaa
     3661 acccgatgat ttgggtttca gacggcattt tttacggtcg caccgccgcc ccctttcagg
     3721 cttgtaccaa gcccaatccc gcaactctcc gtcattcccg caactctccg tcattcccac
     3781 ggaagtggga atctagaaac gcaaagctgc aagaatttat cggaaatgac tgaaactcaa
     3841 cgaacctgga ttcccgcctg cgcgggaatg acgaagctat ccgcacagaa acctgcacca
     3901 cgtcattccc acgaaagtgg gaatccagaa cgtaaaatct gaagaaaccg ttttatccga
     3961 tacgtttccg caccgacaga cctagattcc cgctttcgcg ggaatgacgg cggaaaggtt
     4021 gctgtttttc cgataaattc ctgccgctct tcgtttttgg gatggcggga aataaaacaa
     4081 aagcgcgcgt atcaaaaaac aacaatgcaa agaacggtgt tggcaatttt aaggtttgcg
     4141 ccttagccgc acttattcgc aaaaaaaccg cacggcgttg accgtgcggc ttgttgtctg
     4201 aaggcttcag acggcattgc ttacatcatg ccgcccatac cacccatgcc gcccatatca
     4261 ggcatagccg gtttgtcttc agggatttca gcaatcatgc agtctgtggt cagcatcagg
     4321 ccggcgatag acgcggcgtg ttgcagcgcg gaacgggtta ctttggcggg gtcgagtacg
     4381 cccatttcga tcatgtcgcc gtattcgccg ctgccagcgt tgtaaccata gttgcctttg
     4441 ccttccaaca ctttgttcac aaccacgctg ggttcgccgc ctgcgttggc aacgatttgg
     4501 cgcagcggag actcaacggc gcgcaagacg atttgtacgc ctgcgtcttg gtcggcattg
     4561 ccggtgtgca ggttttccaa agcagcacgg gcacgcaaca gggctacgcc gccgcctgca
     4621 accacgcctt cttcaacggc tgcgcgggta gcgtgcagcg cgtcttccac gcggtctttt
     4681 ttctctttca tttcgacttc ggtcgcggca ccgactttga tgactgccac gccgcctgcc
     4741 aatttagcca cgcgctcttg cagtttttct ttgtcgtaat cgctggttgc ggtttcgatt
     4801 tgttggcgga tttcggcaac acgcgcttcg atttgggctg cgtcgccaaa gccgtcgatg
     4861 atggtggtgt tttctttacc gatttcgatg cgtttggctt gacccaagtc gtccaaagtc
     4921 gctttttcca aagacagacc gacttcttcg gaaatcacca cgccgccggt caggatggcg
     4981 atgtcttgca acatcgcttt gcggcggtcg ccgaagccag gggctttgac ggcaacggtt
     5041 ttcaggatgc ctcggatgtt gttcacgacc aaagtcgcca aggcttcgcc ttctacgtct
     5101 tcagcgataa tcaacagcgg acggctggct tttgccactt gttccaaaac aggcagcagg
     5161 tcgcggatgt tgctgatttt tttgtcgaac aacaatacaa acggattgtc caaagcagcg
     5221 atttgttttt ccgcatcgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     5281 ccttcaacta cgtccagctc gttttccaaa gacttgccgt cttcaacggt aatcacgcct
     5341 tctttgccga ctttttccat cgcttcggcg ataatcgcgc cgacttgttc gtcggagttg
     5401 gcggaaatcg agccgacttg ggcgatttct ttagaagtgt cgcaaggttt ggcgatgttt
     5461 ttcagctctt caaccaaagc ggcaacggct ttgtcgatac cgcgtttcag gtcggtcggg
     5521 ttcataccgg cggtaacgta tttcatacct tcggcaacga tggattgcgc caatacggtg
     5581 gcggtagtcg taccgtcgcc cgccacgtcg ttggttttgg acgcgacttc tttcaccatt
     5641 tgcgcgccca tattttcaaa cttgtctttc agttcgattt ctttggcgac ggttacgccg
     5701 tctttggtga tgtgcgggcc gccgaaagcg cggtcaacca ccacgttgcg gcctttggga
     5761 cccaaggtta cgcggacggc gtttgccaga atgttcacgc cgtttaccat tttttggcgg
     5821 acttcattgc cgaactgtac gtcttttgct gccatttcaa ttctccaaaa atcattaaaa
     5881 ctgtctgata aaaccgttta tgccgtctga aggcggtttg ccgtttcaga cggcatcgtg
     5941 tccgtattta tttttcaacg atgccgaaga tatcttcttc gcgcattacc aacagctctt
     6001 cgccgtcggc ttttacggtt tggccgctgt atttgccgaa aatgattttg tcgccgactt
     6061 tgacatccag cggacggcgc gcgccgtctt taccgatttt gcccgcaccc acggcgatca
     6121 cttcgcccat gtcgggtttt tcggcggccg cacccggcaa aacgatgccc gatgcggttt
     6181 tctcttcagc ttccaagcgt ttgacgacaa cgcggtcgtg taaaggacgg atggtcatat
     6241 ttatgctccg ataaaatgat ttgaaaacaa tcatctgccc gaacggctcg gacagattga
     6301 agtggaaacc gggctgccgc ctgccggccc gcccgtataa gtcggcaaaa ttagggtgtg
     6361 tgcgggcaaa ttcaagtgag gcggaaaaaa tttatttccg ccgtttttta tagtggacta
     6421 aatttaaggg gctgtactag attagcagat atgttaccct cgaaatatga agataacgca
     6481 ctgcaaatta aagaaaaaag tacagaaaga actgctccgt ttttttgtac tcgaagttac
     6541 cgccc
//
LOCUS       DAWFJW010000107         6447 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFJW010000107 DAWFJW010000000
VERSION     DAWFJW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.107"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            150..1691
                     /locus_tag="WGR19_001717"
     CDS             150..1691
                     /locus_tag="WGR19_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0799207.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     QTETL"
     gene            1720..2319
                     /locus_tag="WGR19_001718"
     CDS             1720..2319
                     /locus_tag="WGR19_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0799208.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2316..2921
                     /gene="nadD"
                     /locus_tag="WGR19_001719"
     CDS             2316..2921
                     /gene="nadD"
                     /locus_tag="WGR19_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0799209.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            2980..3366
                     /gene="rsfS"
                     /locus_tag="WGR19_001720"
     CDS             2980..3366
                     /gene="rsfS"
                     /locus_tag="WGR19_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0799210.1"
                     /translation="MNEQELQDLQKMVEVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3420..3890
                     /gene="rlmH"
                     /locus_tag="WGR19_001721"
     CDS             3420..3890
                     /gene="rlmH"
                     /locus_tag="WGR19_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0799211.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            3963..4229
                     /locus_tag="WGR19_001722"
     CDS             3963..4229
                     /locus_tag="WGR19_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0799212.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWMAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4386..5069
                     /locus_tag="WGR19_001723"
     CDS             4386..5069
                     /locus_tag="WGR19_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0799213.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5193..5705
                     /gene="coaD"
                     /locus_tag="WGR19_001724"
     CDS             5193..5705
                     /gene="coaD"
                     /locus_tag="WGR19_001724"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0799214.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5737..6393
                     /locus_tag="WGR19_001725"
     CDS             5737..6393
                     /locus_tag="WGR19_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0799215.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
BASE COUNT         1405 a         1698 c         1861 g         1483 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttcagacggc gggaggaagg
       61 cggatacggt atgtagtcgg attgaaatat acggtatgtg ggggcttttg ggtacaatcg
      121 caacatcagt gtttcaagtg gggaaggcga tggatggacg gcgttgggtg gtatggggtg
      181 cttttgccct gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg
      241 tggcggcgta tgacggtttg gcgtggcgcg cggtgctgtc ggatgcctat atgctcaaac
      301 gtttggcgtg gacggtattt caggcagcgg caacctgtgt gctggtgctg cctttgggcg
      361 tgcctgtcgc gtgggtgctg gcgcggctgg cgtttccggg gcgggctttg gtgctgcgcc
      421 tgctgatgct gccttttgtg atgcccacgt tggtggcggg cgtgggcgtg ctggccctgt
      481 tcggggcgga cgggctgttg tggcgcggca ggcaggatac gccgtatctg ttgttgtacg
      541 gcaatgtgtt tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag
      601 tgcctgcggc acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt
      661 tggacattga aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtcttcc
      721 tgtattgttt ttcggggttc gggctggcat tgctgctggg cggcagccgt tatgccacgg
      781 tcgaagtgga aatttaccag ttggtcatgt tcgaactcga tatggcggtt gcttcggtgc
      841 tggtgtggct ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca
      901 ggcgcgcggt ttcggataag gcggtttccc ctgtgatgcc gtcgccgccg cagtcggtcg
      961 gggaatatgt gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt
     1021 tgttggcaat tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa
     1081 gtgaaacgtg gcaggcggtg tggaatactt tgcgcttctc ggcggcggcg gtgtatgcgg
     1141 cggcggtttt gggtgtggtg tatgcggcgg cggcgcggcg gtcggcgtgg atgcgcgggc
     1201 tgatgttttt gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt
     1261 atccgcagtg gacggcttcg ttgccgctgc tgctggcgat gtatgcgctg ctggcgtatc
     1321 cgtttgtggc aaaagatgtt ttatcagcct gggatgcact gccgccggat tacggcaggg
     1381 cggcggcggg tttgggtgca aacggctttc agacggcatg ccgcatcacg ttccccctct
     1441 tgaaaccggc gttgcggcgc ggtctgactt tggcggcggc aacctgcgtg ggcgaatttg
     1501 cggcgacatt gtttctgtcg cgtccggaat ggcagacgct gacgactttg atttatgcct
     1561 atttgggacg cgcgggtgag gataattacg cgcgggcaat ggtgctgaca ttgctgttgg
     1621 cggcgttcgc gctgggtatt ttcctgctgt tggacggcgg cgaaggcgga aaacagacgg
     1681 aaacgttata atgtgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc
     1741 cttgcccata accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa
     1801 taccccgaac gcgggctggc agttttgtcc tgtatggatg cgcggattat cgggctgctg
     1861 cccgacgcgt tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg
     1921 gttacgcacc cgtggggttc ggtgatgcgg agtcttttgg ttgccgtgtt tgaattgaag
     1981 gtcagagaga ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa
     2041 gaattcctcg ggcgcgtccg ggaaagccgg attcccgaag accgtatcga aaccctgcgt
     2101 tatgcaggtg tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg
     2161 cgccacacgg tggaccttat ccgtaaccat ccgctgatgc cgcgccatat cgccgttcac
     2221 ggactggtca tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca
     2281 gacggcatgg acttatcgga aggaatggaa acatcatgaa gaaaatcgga ctgttcggcg
     2341 gcactttcga cccgatacac aacgggcatc tccacatcgc ccgcgccttt gccgacgaaa
     2401 tcggcttgga cgcggttgtt ttcctgccga caggcggccc gtatcacaaa gacgcagcct
     2461 ccgcttccgc cgccgaccgc cttgccatgg tcgaactggc gacggcagaa gacgcgcgtt
     2521 ttgccgtcag cgattgcgac atcgtccgag aaggtgcaac ctatactttt gataccgtcc
     2581 aaatcttccg ccagcagttc ccatccgcgc aactctggtg gctgatgggc agcgacagcc
     2641 tgatgaagct gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg
     2701 tcgccatgcg gcagggcgac agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg
     2761 gcaagtccct tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt
     2821 caacggaaat ccgccgcaac cttgccggcc aaggcgtttc agacggcatc ccgcctgccg
     2881 ccgcacgcta catccgcgaa cacggtttgt atgaaaaata aagtcaaatc agtaagaaac
     2941 ggctataatg ccgtctgaaa acatcccgtt aggaacataa tgaacgaaca agaactgcaa
     3001 gacctgcaaa aaatggtcga ggtcgccgtc aacgccctcg aagacatcaa agccaaagac
     3061 atttccgttc tcgaaacgca agacaaaact tcgctgtttg ccagaatgat tatcgccagc
     3121 ggcgacagta cgcgccaagt caaagcactg gccaacaacg ttgccgtcga tttgaaagaa
     3181 gccggttttg aaatcctcag caccgaaggc gacagcggcg aatggacgtt ggttgatgca
     3241 ggagacctcg tcgtccacgt tatgctcccc gccgtgcgcg acttctatga tatcgacacc
     3301 ctttggggcg gcgagaaacc gagtttccac gccggaatgc agaagccgtg gcacgcggca
     3361 gactgattcc cgatgccgtc tgaatgttca gacggcattt tcctgtaagg gagaaagcat
     3421 tgaacatcac cgttttggca gtcggcacca aaatgccgcg ctgggttgat gaggccgtcg
     3481 ccgaatacgc caaacgcttc ggacgcgacg tcgcctacgc actcaaagaa atcaaacccg
     3541 aaaaacgcgg cgcgggcgtg aatgccgccc aaggtatggc ggcggaagaa aaacgcatcc
     3601 ttgaagccat tccgcaaggc gcgttcctcg tcgttcttga cgaacgcggc aaagcaccga
     3661 cctccgtcga gctggcggaa cacctcaaaa gctggcagca aaacggcgaa cacgtctgct
     3721 tcgtcatcgg cggcgcggac ggtatgaccg accgcctcaa acagcaggca cgcatgatga
     3781 tgcgcctgtc cagcctgacc ctgccgcacg ggatggtgcg cgtccttctg accgagcagc
     3841 tctaccgcgc cgcctccatc ctgcacaacc atccctatca tcgggaataa gaaggctttt
     3901 gtccgtatcc ctaatcggtt aaaatcaccc cgttatttct gaacacagca aaggaatccg
     3961 ctatggcacg aatggtattc tgcgtcaagc tcaacaaaga agccgaaggc atgaaatttc
     4021 caccgcttcc caacgaattg ggcaagcgca tttttgaaaa cgtatcgcaa gaagcatgga
     4081 tggcgtggac gcgccaccaa acgatgctga ttaacgaaaa ccgcttaagc cttgccgatc
     4141 cgcgcgcgcg cgaatacctg gctcagcaga tggagcagta tttcttcggc gacggcgcgg
     4201 atgccgttca gggatacgtt ccgcaataac ggttttccgt tttgaacaca ggctgtccga
     4261 aactgcttca gacggccttt aaaatacgcc ggacaacttt atcttcacgc aaccggacaa
     4321 attttgacat tgggcaatgt taaaatcccc cattattccc aaccgcgctt tcaggagcag
     4381 atgatatgca acacgacgtt tacgactaca ccgcgcatac ggtttctaaa aacaccgtcc
     4441 tgcagaaaac ctaccgcctg ctcggatttt cattcattcc ggctgccgca ggcgcggcac
     4501 ttgccgccaa tgccggtttc aatttttacg ccgccttcgg ttcgcgctgg atagggtttg
     4561 ccgtcgtgtt ggcgtttttc tacggtatga tccacttcat cgagaaaaac cgttacagca
     4621 ataccggcgt taccctgctg atggtattca cattcggtat gggcgtattg atcggccccg
     4681 tgctgcaata cgcgctccat attgccgacg gtgcgaaaat cgtcggcatt gccgccgcga
     4741 tgaccgccgc cgtcttttta acgatgtccg ccttggcgcg ccgaacccgg ctcgatatga
     4801 acgcgctcgg acgcttcctg accgtaggtg cggtcattct gatggtcgcc gtggttgcca
     4861 atctgttttt gggtattccc gcactcgccc tgaccatttc cgccggtttt gtcttgttca
     4921 gttccttaat gattatgtgg caggtacgca ccgtcatcga cggcggcgaa gacagccaca
     4981 tcagcgcggc actgacactg tttatctcgc tttacaacat cttcagcagc ctgctgaaca
     5041 tcctgttgtc tttaaacggc gaggattgat gccggtaaaa cgccgtccga ctatgccgtc
     5101 tgaaaatgct tcagacggca ttttactttg ggcatacaat acggatgcac acgcaccaac
     5161 acacagccga ctatgaagcc gggaggaatc aaatgttacc gaatacaccg cgccgcgccg
     5221 tttatgccgg cagtttcgat ccgcccacat tggggcatct gtggatgata cggcaggcgc
     5281 aatctatgtt tgacgaactc atcgtcgcca tcggcattaa ccccgacaaa cgcagcacct
     5341 ataccgtcgc tgaaaggcag gatatgttgt gcgatattac taaaatgttt cccaacgtca
     5401 gaaccgatgt atttgaaaac cgatttctgg tgcattatgc ccgtgaggta gatgcaggat
     5461 tcatcgtgcg cggcatccgt tctgcttcgg attacgaata cgaacgttcc atgcgccaca
     5521 tcaacagcga cctcgccccc gaaatatcca ccgtattcct catgccgccg cgcgaaatcg
     5581 ccgaagtgtc gtccactatg gtcaaaggac tggtcgggcc ggaaggctgg acggaaacga
     5641 tccaccgcta cgtgccgcaa gctgtgtacg aaaaaatcct tgccgaacat caacacgaaa
     5701 attgaaccct tctatttttg caaggcatat atggatatgt tggaaatttt gttccgccat
     5761 caagacttcg tcgccatcaa caaaccgggc ggcatatccg tccaccaaga cagcggcgag
     5821 accggactgg cccgaacact cgccatacag ttgggcgtag agcgtgtgtg gctgctgcac
     5881 cgattggaca aacagaccag cggcatttta ctgtttgccc tcaacaaaga aagcgcgtcc
     5941 gcccttagcg gacagtttgc cggcaaaagc atcaaaaaaa cctatttggc actgtccgac
     6001 cgcaagccgt ccaaaaagca gggttggatt aaaggcggaa tggaaaaatc cagatgtgga
     6061 atgtggaagt tgacgcgcaa tacagaaaat atcgccgtta cccgattcca cagcatcagt
     6121 atcgccgaaa aactgcggct gttcatccta gaaccgcata cgggcaaaac gcaccaattg
     6181 agagtggcga tgaaaagttt gggcagtccg attctgggcg acagcttata cggcggaaca
     6241 gaatccgaaa ccatgtttct gtatgcatgg aaaatacaat ttgactacca aaaccgacaa
     6301 atcgaaattg ttgcaccctt aaaaaacgaa tggcagactg aaaatatcca tcgggcactg
     6361 gaagaattct gtatggaaaa taaaattgac tgatataaat taaatttata aacaaataga
     6421 taaatatttt taaaaaaatt tcttcag
//
LOCUS       DAWFJW010000108         6338 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFJW010000108 DAWFJW010000000
VERSION     DAWFJW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.108"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..181)
                     /locus_tag="WGR19_001726"
     CDS             complement(<1..181)
                     /locus_tag="WGR19_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0799216.1"
                     /translation="MLKTLTFAALHFSVAFSITYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLTKTSTALPRLSS"
     gene            315..1268
                     /locus_tag="WGR19_001727"
     CDS             315..1268
                     /locus_tag="WGR19_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0799217.1"
                     /translation="MNQKKVVVLDADTLPGRTFHFDFPHELAVYGTTDATETAVRVCG
                     AHIVITNKVVISADIIADNPQLELIAVSATGVNNVDIGAAEAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLARYAQAFGMRVVFAEHKHAPDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1343..3400)
                     /gene="metG"
                     /locus_tag="WGR19_001728"
     CDS             complement(1343..3400)
                     /gene="metG"
                     /locus_tag="WGR19_001728"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0799218.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPKDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGKLKDVSDSALLAKLTAQSEAIAECYENREYAKALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTILTAYLAPVLPQTAAN
                     AARFLNLDAITWANTRVTLAEGHAINKYEHLMQRVEQKQVDDLIEANKQSIQTTPAPV
                     EDSKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(3516..5354)
                     /gene="glmS"
                     /locus_tag="WGR19_001729"
     CDS             complement(3516..5354)
                     /gene="glmS"
                     /locus_tag="WGR19_001729"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0799219.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGNIK
                     RVRRVGRVQLMEDAAREKHLNGSLGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEDLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIATWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHTALARGTDVDKPRNLAKSVTVE"
     gene            5637..6155
                     /locus_tag="WGR19_001730"
     CDS             5637..6155
                     /locus_tag="WGR19_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799220.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPTLLF"
BASE COUNT         1377 a         1489 c         1895 g         1577 t
ORIGIN      
        1 ttgagctaag gcgaggcaac gccgtactgg tttttgttaa tccactatag aaaacgacgg
       61 tgttgattag gggctcgacc agcgcgaccg ccccggatac gcctatactg cccgtcagta
      121 cataggttat actgaaggcg acgctgaaat gcagtgcggc aaaagtcaag gttttaagca
      181 tcatcatctc cttgattgaa aaattaattg aaaatttacg gagagatgat aaagattatc
      241 ataaggctgt gcggtttaaa tttgctattt gctgttagtg taaataaatc attttctaaa
      301 tagggataaa aattatgaat cagaagaaag tagtcgtatt ggatgcggat actttacccg
      361 gtcggacttt tcacttcgat ttcccacacg agcttgcggt ttacggcacg acagatgcta
      421 cggaaacggc ggtacgcgta tgcggtgcgc acatcgtcat caccaataag gttgtcattt
      481 ctgccgatat cattgcggat aatcctcagc ttgagctgat tgccgtcagt gcgaccggcg
      541 tgaacaatgt cgatattggg gcggcggagg cggcgggcgt tgcggtatgc aatgtccgcg
      601 cctacggaaa cgaatcggtt gcggaacacg cctttatgct gatgattgcc ttgatgcgga
      661 atctgcctgc ctatcagcgt gatgttgcgg caggattatg ggaaaagtcg ccgtttttct
      721 gccattacgg cgcgccaatt cgggatttga acggtaaaac gctggcggtt ttcggacgcg
      781 gcaatatagg acggacgctt gcccgatatg cacaggcgtt cggtatgagg gtggtgtttg
      841 ccgaacacaa acacgcgccc gacgtgcgcg aaggctatgt ttcctttgaa gatgcggtac
      901 ggactgccga cgtgttgtcg ctgcactgtc cgctaaacgc ccaaactgaa aatatgatag
      961 gcgaaaacga attgcggcag atgaagcccg gtgcggtttt gattaattgc gggcgcggcg
     1021 ggctggtgga tgaaaacgcg ctgcttgccg cactcaaata cgggcagatc ggcggggcgg
     1081 gtgtcgatgt tttgacgaat gagccgccca aaaacggcaa tcccttgctg aatgcacgat
     1141 tacccaatct gattgttacg ccgcataccg cgtgggcaag tcgtgaggct ttggacaggc
     1201 tgtttgatat attgttggcg aacattcacg cctttgtgaa aggagaggcg caaaaccgcg
     1261 tggtttgagc ttgtcgggat gacggaaaac aatgccgtct gaacgcccaa agggttcaga
     1321 cggcattttt tgagattcct gtttaaccga ctttgtcgcc cggctgcgcg cctgtatcca
     1381 catctaagag cttcagtttc ccgtctgccg tggcggcact caaaatcatg ccttcggata
     1441 cgccgaattt tgccattttg cgcggggcga agttggcgac ggcgatgacc attcttccgt
     1501 ttaactcggc agggttggga taagacgcgg cgataccgga gaagatgatg cgtttttcaa
     1561 aaccgaaatc gaggtcgaat ttcaaaagtt tggtgctgcc ttcgacggct tcgcagttca
     1621 atactttggc gacgcgcatg tcgattttca taaagtcgtc gaaacttgcc tgttcggcga
     1681 ctttttcgta tttgctgtct tcaacaggcg cgggggttgt ctgaatactt tgtttgttgg
     1741 cttcgattaa atcgtccact tgtttttgct ccactcgttg cattaaatgt tcgtatttgt
     1801 tgatggcgtg tccttccgcc aaagttaccc ttgtgttcgc ccaagtaatc gcgtctagat
     1861 tgaggaaacg cgcggcgttg gcggcggttt gcggcaacac gggggcgagg taggcggtca
     1921 gtatggtgaa ggcgttgatg agctcgctgc atacttcgtg caggcgttcg tcttgacctt
     1981 cttgtttggc gagttcccac ggtttgttgg catcgacgta ttcgttcacg gcatccgcca
     2041 aagccataat gtcgcgcagg gctttggcgt attcgcggtt ttcgtagcat tcggcgatgg
     2101 cttcgctttg tgcggtcagt tttgccagca atgcgctgtc ggaaacgtct ttgagttttc
     2161 cttcaaagcg tttggcgatg aaacctgacg cgcgggcggc gatgttgacg tatttgccga
     2221 cgaggtcgct gttgacgcgc gaaataaagt cttgcagatt caaatcgatg tcttcgattt
     2281 tgctgttgag tttggcggcg atgtagtagc gcatccactc ggggtttagg ccttgttcca
     2341 gataggattt ggcggtgata aacgtgccgc gcgatttgga cattttttgt ccgtctacgg
     2401 tcaaaaagcc gtgtgcgtac acgccggtcg gggcgcggtg gccggagaaa tgcagcatgg
     2461 caggccagaa cagggcgtgg aaatagagga tgtctttgcc gatgaagtgg tacatctcgg
     2521 tttggctgtc ggctttgaag tattcgtcaa aatcgacgcc gatgcggtcg cacaggtttt
     2581 taaacgacgc catgtagccg acgggcgcgt ccagccagac gtagaagtat ttgcccggcg
     2641 cgtcggggat ttcaaaaccg aaatacggcg cgtcgcggga aatatcccag tcggacaggg
     2701 tggtttcttc accttcgccc agccattctt tcattttgtt gagggcttcg gcttgcagat
     2761 ggggcttgcc gtcgtgcggg ttgttgccgg aagtccatgc tttgaggaag tcggcgcatt
     2821 ctcccagttt gaagaagaag tgttcggatt cgcgcaattc gggtttggct ccggatacgg
     2881 cggaatacgg gttgatcagt tcggtcgggg aataggtcgt gccgcagact tcgcagttgt
     2941 cgccgtattg gtcttgggcg tggcatttcg ggcattcgcc tttgacgaag cggtcgggca
     3001 ggaacatttg tttttcgggg tcgaaaagct gctcgatgac gcggctttcg attttgccgt
     3061 tggctttcag tgcgcggtaa atgtcttggg aaaactgttt gttttcaggg gaatgggtgc
     3121 tgtaataatt gtcgtagccg atgaaaaaac cggtaaagtc ggcgaggtgt tcttcgcgca
     3181 ctttggcaat catgtctttg ggcgcaatgc cttgtttttg cgcggcaagc atcacgggcg
     3241 tgccgtgggt gtcgtcggcg cagcagtagt ggcactcgtg gccgcgcagt ttttggaagc
     3301 gtacccaaac gtcggtttgg atgtgttcga ccatgtggcc gaggtggatg ctgccgttgg
     3361 catagggcag ggcggaggta actaagattt tgcgtgtcat attgtgcttt gcaaacaaag
     3421 ggtaaaggcg gattataccg caaatcaaac ggggaaatgc cgtctgaagc ctgaaaaatc
     3481 gggcttcaga cggcattttt gccaaccggt gggatttatt cgacggttac ggatttcgcc
     3541 aggttgcgcg gtttgtccac atccgtgccg cgtgcgaggg cggtgtggta ggaaaggagc
     3601 tgcacgggga tagtatgcac gacgggggat aattctccga catggcgcgg cgcgcggata
     3661 acgtgcacac cttcggtggc attaaaatta ctgtcgaggt cggcaaagac gaaaagttcg
     3721 ccgccgcgcg cgccgacttc ctgcatattg gctttgactt tgtccaatag gctgtcgttg
     3781 ggcgcgatga cgacgacggg catattttcg tccactaagg caagcggccc gtgcttcagt
     3841 tcgccggcag gataggcttc ggcgtggatg taggtgattt ccttcagctt caacgcacct
     3901 tcgagggcta tcgggtaatg gatgccgcgc cctaaaaaca gcgcgctggt tttcttggca
     3961 aactgttgcg cccaggtggc aatttgcggt tcgaggttta aagcgtgctg cacgctgccg
     4021 ggaagctggc ggagttcttc ggtgtaacgt gcttcgtctt cttcggaaac caaaccgcgt
     4081 accttcgcca gcgttaccgc caaaccgaac agtgcgacca gttgcgtggt aaatgctttg
     4141 gtcgaggcga cgccgatttc cgcaccggca cgggtataaa gcacgaggct gctttcgcgc
     4201 ggcagggcgg attccatcac gttgcagatg gagaggctgt ggcggtgtcc caaggatttg
     4261 gcgtatttta aagcctccat cgtgtccagc gtttcgccgg attgggaaat ggtaatgacc
     4321 agttggtctg gatcggcaat cacgctgcgg tatcggtatt cgctggcaat ttcgacatcg
     4381 gccgggattt tggcgatgga ttccaaccaa tatttggcgg tcagcgcggc gtaataggac
     4441 gtgccgcagg caaggatttt gacgctgcgg atgctttcaa acacgctttt ggcgtttttg
     4501 ccgaagtttt cggggatgaa gccgccgtcg aggaaaacct ccgccgtgtc tgcaatcgcg
     4561 cgcggctgct catggatttc cttttgcata aagtggctgt acggtcccag ttccaaagag
     4621 gcgagcgaga gttcggatac cttgactttg cgttcggcag gcaggccgtt tttatcggtc
     4681 agccttttga tgccgtctga agccagcagc gcgatgtcgc cgtcttcgag gtacgccacg
     4741 cggcgcgtaa aggcgatgac ggcggatacg tccgaagcga taaaggtttc atcgtcgccc
     4801 aaagcgacca aaagcgggca gcccatacgc gccacgacta attcatcagg cttgtcttgg
     4861 gcaataaccg cgatggcgta tgcgccgtgg aaacgtttga ccgcttcctg cacggcttca
     4921 aacagcctgc cgccgttttg cgcgtattcg tgattgatgc tgtgggcgat gacttcggta
     4981 tccgtttgcg attcaaaacg gtatcccaaa tcttccaaac gtttgcgttc gctttcaaag
     5041 ttttcgatga tgccgttgtg cacgaccgca atcataccgc cgctgatgtg cgggtgggcg
     5101 ttcggctcgg taacgccgcc gtgtgtcgcc cagcgcgtat gcccgatgcc caaactgccg
     5161 ttcaaatgct tttcacgcgc cgcatcttcc attaactgca cgcgcccgac acggcgcacg
     5221 cgcttgatgt tgccgtcggt gttgacggca atgcctgatg agtcataacc ccggtattcg
     5281 aggcgtttga ggccgtcggt cagaaaatcg acgacattgt gatgggcgcg gatggcgccg
     5341 acgataccgc acataactgt tccttagtat ccggttgaaa aaaaaacagg cgcggacggc
     5401 tcccgtgccg caccttccgc ttcggattat aaaccgcctc ccgcgccgga aaacagcaaa
     5461 atgccgtctg aaggcttggg cttgctcaaa aaagggaggg atttccccgc ctgaccggga
     5521 tgggcgttca gacggcattg cctgccgccg gtttatagtt tttacaaatc aatattgaca
     5581 agctgaaaaa aaaaaacaat atactcgctc ggtcttaatg ttaacggagt atggaaatga
     5641 aacaaatgct tttggccgtc ggcgtggtgg cggtgttggc gggctgcggc aaggatgccg
     5701 gcggttacga gggttattgg cgcgaaaaat cggacaaaaa agagggcgtg attgccgtca
     5761 aaaaagaaaa aggcaattac ttccttaata aaatcaacgt atttacaggc aaggaggagt
     5821 ctttgctttt gtctgaaaaa gacggcgcgc tttcgataaa cacggggata ggggaaatcc
     5881 cgatcaaact ttccgatgac gggaaagagc tgtatgtcga acgcaggcag tatgtcaaaa
     5941 ccgatgcggc gatgaaggac aaaatcatcg cccatcagaa aaagtgcgga caaacggcac
     6001 aggcatacct cgacgcgcga aaggcgttgc cgtcaaacca aacgtatcag cagcgtcagg
     6061 cggcgatcga gcaattaaaa cggcggtttg aagccgagtt tgacgaattg gaaaaagaaa
     6121 tcaaatgcaa cggcagaccg acattgttgt tttagcgtag gacaacaggg aggatgccgc
     6181 cgtccgaatc ggacgtgcgg tttccgtacc ggtacgggcg ggcaggaatg tccgcctttt
     6241 tttgttcgga cgcgtttgaa tacccgtttg attccgaccg tttgcaaggg gtatttttgt
     6301 tcgggcggaa attatagtgg attaacaaaa accagtac
//
LOCUS       DAWFJW010000109         6237 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFJW010000109 DAWFJW010000000
VERSION     DAWFJW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.109"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(53..427)
                     /locus_tag="WGR19_001731"
     CDS             complement(53..427)
                     /locus_tag="WGR19_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0799221.1"
                     /translation="MIIQNKFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVTYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(600..956)
                     /locus_tag="WGR19_001732"
     CDS             complement(600..956)
                     /locus_tag="WGR19_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0799222.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(1099..1443)
                     /locus_tag="WGR19_001733"
     CDS             complement(1099..1443)
                     /locus_tag="WGR19_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799223.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1602..2003)
                     /locus_tag="WGR19_001734"
     CDS             complement(1602..2003)
                     /locus_tag="WGR19_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799224.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2007..2736)
                     /locus_tag="WGR19_001735"
                     /pseudo
     CDS             complement(2007..2736)
                     /locus_tag="WGR19_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2806..3123)
                     /locus_tag="WGR19_001736"
     CDS             complement(2806..3123)
                     /locus_tag="WGR19_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799225.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3120..3731)
                     /locus_tag="WGR19_001737"
                     /pseudo
     CDS             complement(3120..3731)
                     /locus_tag="WGR19_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(3815..4117)
                     /locus_tag="WGR19_001738"
     CDS             complement(3815..4117)
                     /locus_tag="WGR19_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799226.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDDWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(4140..4409)
                     /locus_tag="WGR19_001739"
     CDS             complement(4140..4409)
                     /locus_tag="WGR19_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799227.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(4430..4636)
                     /locus_tag="WGR19_001740"
     CDS             complement(4430..4636)
                     /locus_tag="WGR19_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799228.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKDLPIR"
     gene            complement(4602..4802)
                     /locus_tag="WGR19_001741"
     CDS             complement(4602..4802)
                     /locus_tag="WGR19_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799229.1"
                     /translation="MASDLLSKQTSDKILGYQTEHRRVRYDINNNIYVLANPKTFKIK
                     TMFKPNLGKKYYDGEFKKDMGN"
     gene            complement(5162..5506)
                     /locus_tag="WGR19_001742"
     CDS             complement(5162..5506)
                     /locus_tag="WGR19_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799230.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(5544..5867)
                     /locus_tag="WGR19_001743"
     CDS             complement(5544..5867)
                     /locus_tag="WGR19_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799231.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(5870..6100)
                     /locus_tag="WGR19_001744"
     CDS             complement(5870..6100)
                     /locus_tag="WGR19_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0799232.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFIENTRTGEKTDFKFKD"
BASE COUNT         1810 a         1177 c          944 g         2306 t
ORIGIN      
        1 cgagtttatc ctagttatcc aggatggtcc catctctttt atagatagct gcttaccaag
       61 tatccttaat tgctaattca agccactctt caaaactatt aaaaaattca tggttttcta
      121 tattatctaa attaatgaca ataaccctag gatttcctgt tacatcatta ccatcaaaat
      181 aagcttccca ttctccatta ctagcgaatg ggataagatt taaatcaggc aggatagaat
      241 ttctaaatat ataagttact tctgcccctt ctgttccata attctcaaac caccagtgaa
      301 aattatgtgg ctgaatataa ggtattaaag atgtatcgtt agagatacga acatactttt
      361 caggataaca aaacctttca ggaagcatat tactaggata taaattaaat ttattttgaa
      421 taatcatttc gttaccttag gatcataaat actaccatta ggaaactggg tagaccaatt
      481 tggtggtgct agttcatttt caatttgttt tttaattcta aattgaatag cttctcccca
      541 tgtttgcggc atctaatgct gcttgctctg catcaatatt actaaaaatt ggattagtct
      601 caaaaatgaa aaattactgt tttttcattt tttatagcat attcaagtac atgaataata
      661 tttttcacta agggctctgt agaaaaatct gaattttgta atttttcttt tacagccaaa
      721 atactttcct tatctattag ccaatccccc tcacttggtc caaataacca attatcggta
      781 atttcagaaa caagtctcca taatccacga ttttgatatt caataatttg attttccgta
      841 atttctacaa ctgtttcaga ttgaatgttt ttataagccc aatcaatcat ttcagtatat
      901 tcactaattg aaacattgtc ttttaaacag aaaattaaca ggttgtgttc attcatttgt
      961 ctaagttcct gatatgagtt tgttgttgta aataatcttt tgcttgctta ccttgtaaat
     1021 taaaggatta accgcacctt ggatgatgcc gcgaatatca tccatacggt taccccacca
     1081 atttttattg gtgtatcgtt aatcatttat ttcaaaaaag ttatttatta tcatattatc
     1141 agggagcagg tcgttaaaaa aaaccccctc aaattccatt aaattagtat ttatccagca
     1201 cataaacgaa ccaaagcgtt ctttccctga gtgtaaatat ttaaaattaa actcaaaata
     1261 agtttttttg cttagaaaat ttaatggttt ttctatagtg aaaaaaaagc ccttactcgt
     1321 tttcgttcga gatactattt taaataaatt tttattttca ataaaataag aatattcatt
     1381 tactaagttg ttatgataaa aaagtaattt tattaaaatg ttttcataat tttctaggtt
     1441 catttctagc tcaatttaaa atgatttcat tgttgccgta ccaatgcgaa atttaccatc
     1501 aacaaaattt cccttcactg atacaggtac accatcaact tgggtagttt tattttcagg
     1561 ctgcctgaaa gaaagatagg caacttatta ctcatcttat gctagccctc aataattgaa
     1621 tacgttgcac tagaaccaat tatttctatg aatgtttgct caaacataaa aatatagtat
     1681 ttaaagaatt ctctattcaa aggaaaataa ctttctttca atatccattc cgcataacac
     1741 tcatcaagaa gttcaaaaaa agattgacca ccattttcat gaagcaaagc tctatctgca
     1801 tcttccgaaa ttaaatccat aaaagtatct ataaaatacg attcatcaac aaccctaaaa
     1861 tgaataacat cttcaataaa aatttttatg gttttatccg tatttctata accaactgct
     1921 ataacttcta ctttatcaga gtttattgct gcattatcaa aatagaaata gccagttggg
     1981 aaatctaagc tttttaattt cataatttat cgtgtatttc catatctgat ttttattttt
     2041 cggttattac taattgttat ttcaagtgtt ggttctccac cactactatc attacgagca
     2101 ttaaccgcac gcccatcagg taaattgccc gttatcgttc cattaggacg tgtttgtaca
     2161 tttcgaacat tcagactatt aaaatcattt agtgcgtcat tgtaatcccc cactttatca
     2221 tactgaacag cgggacctcc tttgagtagt attgctcaaa atgtctttga tttgtaaatc
     2281 actgcgcgcc ttaccggctt ctatgcgagc tgccacacta ctcgggctat aggggtcgct
     2341 atcaatcgca ggattaccac cgctggtttt ttgtccgcta gcctgtttcc caacctgctg
     2401 tactgtatcg ccaatttcat ctaccgtacc taacgcctta gtctgcatat ggcgaacagc
     2461 aggtttttta tagccggtat ttttaaccca atcccatttg gtcgggttaa gttctacttt
     2521 tttacctctc caaaccgtac ctgcggcctc tgctacggca agggcagttt gggctgttgc
     2581 tgttatattc ggatgggcat cagcccattg tctggcggaa ttgatgccgt cttttaccgc
     2641 aaaagcactg tcctgtaata tgctcgcggc agcaagttgt gcttccgggc ttaattttcc
     2701 taaattatta ataccttgta gagtctgctg cgcagagatg tagcaactgt atttttaccc
     2761 cgtcgggtaa aaatacagtt gctacggctg ctgggaaagc agttactatt ttcccagtgg
     2821 ctcaaaatta attgtttcag cagatatttt tataataatc cctacatcaa taataatttc
     2881 actataaaat tggtatttat atagctctat ttcgggtata aacaagggaa acaagctatc
     2941 ttccttatcc tcataatcta cgaaattatt tgtatctaat ttaaaattca aaatattact
     3001 gcatttgata tatccgaaat aattaccttc aattgtatct gtaaaagata atgtaagggt
     3061 atttccatta tcggaaaaga ttatttcata aaggcttaca ttgtgaaaaa tatttttttt
     3121 catttgataa aatatccaga atgatgacga ttatttggca atggattgcc ttcataattt
     3181 tgtcgttgcg gatattttcc ctgtacacca tcggttcgcg gcactctttg ggttcttata
     3241 acctctccat ttggacgaat atctattttc acgccatcag gacctttcca tgtttcatat
     3301 tgagcctcat ctgaaccagt acgtctaaaa ccttgttctt gcaatgcttg acgtaattca
     3361 tctcgcgtag ttggtctgat agatttaaca actggcttgt ttcctcctgc catctcaccg
     3421 tccaacgtct gcataggtcg ggcagcaggt tttttatagc cggtattttt aacccaatcc
     3481 catttggtcg ggttaaggtt tactttttta ccgccccaaa ccgtacctgc ggcctctgct
     3541 atggcaagag cagtttgggc agtagctgta atgttcggat gggcatcagc ccattgtctg
     3601 gcggaattga tgccgtcttt taccgcaaaa gcactgtcct gtaataggct cgcggcagca
     3661 agttgtgctt ccggacttaa ttttcctaaa tcattaatac cttgtagagt ctgctgagcg
     3721 gctgtatctg agccggtcag tgtaggttgg attaaaaccc agcattttca acatcttgat
     3781 gttttactat aaatctattc ttgtgcttcc tttgttagat aaatttcaac acaaaagata
     3841 cgtatgcatt ctctaatttt atttgagata aataatgcat tgctttgtcg atactgttaa
     3901 ataaattact tccctcgtaa taccaatcat catatgtttg ataaaaataa ttatctttct
     3961 tgatataaat atctcccccc aatacaagta ttctctcatt gataaataaa tttaatatat
     4021 ctattgcaat agggacgggt aatgcggctt ctctaattcc aattcctatc aaagattccc
     4081 cttgtctcga ataaatttca aaaatttcat ttaacatgtt aatctctatc tactattttt
     4141 caatcggtat aaaccctatt cacagtttca tcttgtattt ctttatgaat acttaatata
     4201 ttgagatagc ttaggaattt tattttctca gattgaggca actcatcaaa accaagccaa
     4261 caataaaaat tgttatatga ttttaaaaaa tcaacaattt ttatttttcc taaaaccaaa
     4321 tcttcgacta actgggttgc ataaataata tcatttatga ttttaacagg taaattattt
     4381 gataggtaac catgggattc cgtcatcatt gatttctcta ggtgcttatt tatctgattg
     4441 gtaagtcttt tgcgtaagct tctttcgcct ccgcaagtgt catttcatta gggccaccat
     4501 ctatattagt ttctcctgta ttttgccact gacaaacgtc acagatatca tagtccataa
     4561 cttcagttcc gcaaacagga caatgtagcc atatttctcc gtcaatttcc catgtctttt
     4621 ttgaattctc catcataata cttctttcct aagtttggtt taaacattgt tttgattttg
     4681 aatgtttttg gattggccaa aacatagata ttgttattga tatcatagcg cactcgtcta
     4741 tgttccgtct gataacctaa tatcttgtcc gatgtctgtt ttgataacaa atcagacgcc
     4801 attttttggt agtcttcaat ggttatatcg ccaaattctt ttccatgagc tttgaaatgt
     4861 ccatttaaag atttctcagt aggaaacttg gactttgtcc atctgatgcg gtcttgaatt
     4921 gttcctgtcg gagaaataga tgtatcaggt ttaacatatg aggcaggtaa gccgatattg
     4981 ttatcagagt gtgtagggtg ttgctgcgta cttggttttg gtggcttatt cccacctgcc
     5041 atctccccat ctaaagtctg catagggcgg gcagtaggtg ttttatagcc ggtattttta
     5101 acccaatccc atttggtcgg gttaagttct acttttttac cgccccaaac cgtacctcca
     5161 gtcaggtgta gttggatggg gattcatttt taataatatc ttcaagcaag tgaaatgtaa
     5221 gaaaagcgta gatacttgaa ggccggctat ctgattggca aaaagaaatt tcggagataa
     5281 agaatttccg tttatatttt tcataaatta aatcaacacc ttgttttaca taaaaactaa
     5341 cttcctcagg aaggatattt ataacattct ctccttttac ttttatagtt aatggtttga
     5401 tttttatgtt ttcatgaata tctgatagga ttatttctaa aaaattattt ttatttccag
     5461 tcattctggc tattttatgt atatttccat tgtgttcgat tatcatttta ttttttccaa
     5521 ggatttcctt ctatattgat agtttatcta aagatttcat ttaaaatatc ctgactaatt
     5581 tcttgtttat ataattctac aattttctgt ttctgtgtat ttgtataaaa tttttcgttg
     5641 gtatctgctt gatattttaa ttgtctcaat atttccatga tttcatattc tgatttttca
     5701 tcatattctt caatcatgta atcaacaaat tgttttagaa tatcaaaggc ttcgtaactt
     5761 tccaaaattt taatatgttt atcggcaaaa ttggacatat accattcgtc agtgattgga
     5821 ttagtattta aatcatctaa aaaaacattt acatcatcta aaaacatgac taatccttaa
     5881 acttaaaatc tgttttctca ccggttcttg tgttttcaat aaacttaaaa tctgtttttt
     5941 caccggttct tgtgttttca atgtaatgta tttcaatttt gtttacgttt tgaacacgct
     6001 taacccaacc gtcttttgct aaccaaccat ttttagtatc ggacatagga ggtattctcg
     6061 caggagtttt gccctgtggg tttgccttaa cttcttccat tgctaattgt tcctttaaat
     6121 tattaggtat tgtacgaccg gttgattttt caacagattg tgtccctatt tttaatgatt
     6181 tgtttccccc agccatttcc ccgtctatag tctgcatatg gcgaacagca ggttttt
//
LOCUS       DAWFJW010000110         6112 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFJW010000110 DAWFJW010000000
VERSION     DAWFJW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.110"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            25..408
                     /locus_tag="WGR19_001745"
     CDS             25..408
                     /locus_tag="WGR19_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0799233.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            523..996
                     /locus_tag="WGR19_001746"
     CDS             523..996
                     /locus_tag="WGR19_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0799234.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            1122..2225
                     /gene="mnmA"
                     /locus_tag="WGR19_001747"
     CDS             1122..2225
                     /gene="mnmA"
                     /locus_tag="WGR19_001747"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0799235.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVCNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKEGHYTCKTRYRMADAPCELRYLDDETVELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2296..3966
                     /locus_tag="WGR19_001748"
     CDS             2296..3966
                     /locus_tag="WGR19_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0799236.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLIK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4078..5712
                     /locus_tag="WGR19_001749"
     CDS             4078..5712
                     /locus_tag="WGR19_001749"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0799237.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSENIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5727..5929
                     /locus_tag="WGR19_001750"
                     /pseudo
     CDS             5727..5929
                     /locus_tag="WGR19_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5940..6112
                     /locus_tag="WGR19_001751"
                     /pseudo
     CDS             5940..6112
                     /locus_tag="WGR19_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1608 a         1670 c         1526 g         1308 t
ORIGIN      
        1 tgagaacatt ttgaaaggat accgatggaa ccttcctcct acgcggcaga aaaaaaaggc
       61 aaaggcggca tcaggcgcgt cattaacgca ttcggctatt cgatagacgg catcgccgcc
      121 gcctaccgtt acgaagcggc attccgtcag gttttgtggc tgaacgtact gctggtgtgc
      181 gcggcatttt tttgggtttc cgaaaccgcc gtccgcctgc cgttgattat cgcgtctttt
      241 gtgtcggtca ttgtcgaact gttcaacacc gccgtcgaag ctgccgtcga tcatacttcg
      301 accgaaaaac acgagctggc caaacgcgcc aaagacgcag gttctgccgc acaattgttc
      361 gcgatgctga tgttggcggc ggtttggctg tccgccctgt tcgggtaaaa cgcttgcagc
      421 aggatttgta atcctttagg attagtatgt agtatttatt attcacttaa tccactataa
      481 gattaagcac attccccgtc tgatattata aggagcagaa agatgaaaaa attattggcg
      541 gccgtgatga tggcaggttt ggcaggcgcg gtttccgccg ccggagtcca cgttgaggac
      601 ggctgggcgc gcaccaccgt cgaaggtatg aaaataggcg gcgcgttcat gaaaatccac
      661 aacgacgaag ccaaacaaga ctttttgctc ggcggaagca gccccgttgc cgaccgcgtc
      721 gaagtgcata cccacatcaa cgacaacggc gtgatgcgga tgcgcgaagt caaaggcggc
      781 gtgcctttgg aggcgaaatc cgttaccgaa ctcaagcccg gcagctatca cgtgatgttt
      841 atgggtttga aaaaacaatt aaaagagggc gacaagattc ccgttaccct gaaatttaaa
      901 aacgccaaag cgcaaaccgt ccaactggaa gtcaaaatcg cgccgatgtc ggcaatggac
      961 cacggtcatc accacggcga agcgcatcag cactaatctg ctgaaaatat ttgaaatgcc
     1021 gtctgaaaaa gcccgggctt tcagacggca tttttatgac cgcctttaaa atgtgttaaa
     1081 atctgccttt aaaaaccgcc gtttccaacc atattctacg catgaatacg acagcaaacc
     1141 cttccaatat catcgtcggg ctctccggcg gtgtcgattc ttccgtaacc gccgccctgc
     1201 tcaagcagca gggttatcaa gtgcgcggtg tgttcatgca aaactgggag gatgacgaca
     1261 acgacgaata ttgcagcatc aaacaggatt cgtttgatgc catcgccgtt gccgatattg
     1321 tcggcatcga tatcgacatc gttaatttcg ccgcgcaata taaagacaaa gtctttgctt
     1381 attttcttca ggaatacagt gcggggcgca cgccgaatcc ggatgtgttg tgcaacgccg
     1441 aaatcaaatt caaatgcttt ttggattacg ccgtagggca gggcgcggat accatcgcca
     1501 ccggccacta tgcgcgcaaa gaagtctgca acggcgtgca ttacctgctc aaaggtttgg
     1561 atcgaaacaa agaccaaagc tatttcctct accgcctcaa gcctttccaa ctcgaacgcg
     1621 cgattttccc gttgggcggt ttggaaaaac ccgaagtacg ccgccttgcc gccgagttca
     1681 aactgccgac cgccgccaaa aaagacagca cgggcatctg cttcatcggc gagcgtccgt
     1741 tccgcgagtt cctacaaaaa tacctgccga ccgacaacgg taaaatggtt acgcccgaag
     1801 ggaaaaccgt cggcgaacac gtcgggctga tgttctacac gctcggacag cgcaaaggtt
     1861 tgggcatcgg cggcgcgggc gaaccgtggt ttgttgcggc taaagatttg acgaaaaacg
     1921 aactcatcgt cgtacaaggt catgaccatc cgctgctcta tacccgcagc cttgtgatga
     1981 acgatttgag tttcacgctg cccgagcgtc cgaaagaagg gcactatacc tgcaaaacgc
     2041 gttaccgtat ggcggacgcg ccttgcgaat tgcgctattt ggatgatgaa accgtcgagc
     2101 tggtgtttga cgaaccgcaa tgggcggtta cgccgggcca gtctgccgtg ctgtacgacg
     2161 gcgacatctg cttgggcggc ggtatcatcc aaacgaccga caaacccgtc atcatcacac
     2221 gataaaactt atgccgtctg aaacggtttt cagacggcat tgttccgctc aattccactt
     2281 taaagaccga tacctatgga aaaaatctgg ttagacagct acgagaaggg cgtcagtgcc
     2341 gaaatcgaca tcacgcaata caattccgtc agcgacgtat tccgtcaaag cgtggaaaaa
     2401 tttgcccgtc tgcccgcttt tcaaaatatg ggcaaaacgc tgacttatgc cgaaaccggc
     2461 aaactggcaa cagatttcgc ctcttatctg caaaacgtcc tcaagctgcc gcgcggcgaa
     2521 cgtgttgcca ttatgatgcc gaacgtattg caatatccga ttgcactttt cggcattttg
     2581 caggcaggtt tggtggcggt gaacaccaat ccgctctata caccgcgcga gttggagcat
     2641 cagctgaaag acagcggtgc gaccgccatc atcgttttgg aaaatttcgc caacacgctg
     2701 gagctggtgc tgccgcgcac gcagatcaaa cacgtcatcg tcgcctccgt cggcgaaatg
     2761 ttcgggctga ttaaaggttc gctgattaat ttcatcatcc gaaaaatcaa gaaaatggtg
     2821 cccgaatacc gcattcggga aaccgtttcc tttcagacgg cattgaaaga gggagcgaag
     2881 cacgttttcc aacctgtcgc attaaaccgc gaagataccg cactattgca atacacgggc
     2941 ggcacgacag gtgttgccaa aggcgcagtg ctgagtcacg gcaacatctg cgccaatatg
     3001 cttcaggcaa aagaatggat taaaaaccag ttgcgcgagg gcaaagaaac cgttatcgcc
     3061 gccctgccgc tgtaccatat ttttgcctta accgtgaatc tgatgatttt tgccaatgcc
     3121 ggttcaaaaa ttgtcctgat taccaacccg cgcgatatga aaggctttat cggagaactg
     3181 aaaaaacagc gggttaatgt atttatcggc gtgaacacgc tgtttaacgc gatggttaac
     3241 cggcccgatt ttgccgaagt cgatttttca ggattgcggc tgactttggg cggtggtatg
     3301 gcgacccaaa aagccgttgc cgaaaaatgg aaaaaaatca ccggcacgcc catcgtcgaa
     3361 gcctacggtc tgaccgaagc cagccccggc gtgtgctgca accccttaaa catcgaatca
     3421 tacagcggca gcatcggttt gcccgtctcg tccaccgaag tcgaactgcg cgacgcaaac
     3481 ggcaaagaag tccccgtcgg gcagccgggc gaattgtggg taaaaggccc tcaagtgatg
     3541 caaggctact ggaaccgccc cgaagaaacc gccaaagcca tagacgcgcg cggcttcttg
     3601 gaaaccggcg atattgccgt catggacgaa aaaggctggt tgaagctggt cgatcgtaag
     3661 aaagacctcg tcgttgtttc cggattcaat gtttatccga acgaaatcga ggaagtcatc
     3721 gcgcatcacg gcaaagtcat ggaagtcgcc tgtatcggcg ttcccgacga aaaaaccggc
     3781 gaggcactca aagtattcgt cgtcaaaaaa gacccgtctt tgaccaaaga agaacttatc
     3841 gctttttgcc gcaccgaatt aaccgcatat aaagtaccga aaaatatcga attccgcgac
     3901 gagttgccca agtccaacgt cggcaaaatc ctgcgccgcg agttgcggca aagtaccggg
     3961 aaataaagaa aagatgccgt ctgaaaacag ccgtccatcg ttcagacggc atccgtccgt
     4021 ttgccagaac cgcgcgcttc acgttaaaat cacgcattcc aatacgggta tttcattatg
     4081 accaaattca ttttcgtcac cggcggcgtt gtctcctcac tgggtaaagg tatcgccgcc
     4141 gcttctattg ccgccatcct cgaatcgcgc ggcttgaacg ttaccatgct caagctcgat
     4201 ccttatatca acgtcgatcc cggcacgatg agcccgttcc aacacggcga agtgttcgta
     4261 accgacgacg gcgcggaaac cgacctcgac ttgggacact acgaacgctt tatcgattcc
     4321 acgatgaccc gccgcaacag cttcagcacg ggacaggtgt acgaaaacgt tatcgccaaa
     4381 gaacgacggg gcgactacct cggcggtacg gttcaagtca ttccgcacat taccgacgaa
     4441 atcaaacgac gcatccacga aggcgcggca ggttacgatg tggcgattgt cgaaattggc
     4501 ggcacggtcg gcgacatcga atcgctgccg tttttggaag ccattcgcca gatgcgaagc
     4561 cagttgggac gcaacaacac cctgttcgcc cacttgagct acgtccccta catcgccgcc
     4621 gcaggcgaaa tcaaaaccaa gccgacccag cacaccgtta aagaaatgtt gagcatcggt
     4681 ttgcaacccg acatcctgat ttgccgcatg gacaggaaaa tgcctgcgga cgaacgccgc
     4741 aaaatcgcct tgttctgcaa cgtggaagaa cgcgcgattg tcggcagcta cgatgtggac
     4801 agcatctacg aatgccccga aatgctgcac gaccaaggca tcgacaacat cattaccgag
     4861 cagttgcagc ttaacgtaca gcaggcggat ttgaccgcgt ggaaaaaaat cgtccacgcc
     4921 gtcaaaaatc cgaaacacac cgtcaaaatc gcgatggtcg gcaaatacgt tgatttgacc
     4981 gaatcctaca aatcactgat tgaagccttg aaacacgcag gcattcacac cgaaaccgac
     5041 gtgcagatta cctttgtcga cagcgaaaac atcgagaaaa acaagggcga cgtttccatg
     5101 cttaaagaca tggatgccat cctcgttccc ggcggtttcg gttcgcgcgg cgtggaaggc
     5161 aaaatcgccg ctgtgcgcta tgcccgcgaa aacaacgtgc catacttggg catctgcctc
     5221 ggtatgcaga ttgcgctgat cgaatacgcc cgcgacgtgg caggtttgaa aggtgcgaat
     5281 tccaccgagt tcgacctcaa atgcgccgcc cccgtcgttg ccctgattga cgaatggcaa
     5341 actgccgacg gcagcgtcga aacccgcgac gaatccgccg atttgggcgg caccatgcgt
     5401 ttgggcgcgc aagaagtcga attgaaagca ggcagcctcg ccgccaaaat ctacggcagc
     5461 gaacacatcc gcgaacgcca ccgccaccgc tacgaagtca acaacaacta cgttcctacg
     5521 ctggaacagg caggtttggt catcggcggc gtatccgccg gacgcgaacg cttggtcgaa
     5581 accatcgaac tgccgaacca tccttggttc ttcgcctgcc agttccaccc cgagtttacc
     5641 tccaatccgc gcaaagggca tcctttgttc accgcgtttg tcaaagctgc gttgaacaat
     5701 aaaaaagcct gataaagcgt tacttgatga tcaaaatgcc gtctgaaagc ctgaatgaag
     5761 actttcagac ggcattttcg caaatcgggg attataaaag ccatcaattt aatttttatg
     5821 gttttatcta atatttacca actgaatatt gttaagcggg gcgtgaaact caagttgaga
     5881 cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg ggatttctca
     5941 tgagcacctt ctttcaacaa accgcccaag ccatgattgc caaacacatc gaccgcttcc
     6001 cactattgaa gttggactag gtgattgatt ggcagccgat cgaacagtac ctgaatcgtc
     6061 aaagaaccca tttaccttag agaccaccgc ggccgtccgc ctatccgctg ct
//
LOCUS       DAWFJW010000111         6078 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFJW010000111 DAWFJW010000000
VERSION     DAWFJW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.111"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            25..1074
                     /gene="siaC"
                     /locus_tag="WGR19_001752"
     CDS             25..1074
                     /gene="siaC"
                     /locus_tag="WGR19_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0799238.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIRETGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            1158..2645
                     /locus_tag="WGR19_001753"
     CDS             1158..2645
                     /locus_tag="WGR19_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ0799239.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(3141..4094)
                     /locus_tag="WGR19_001754"
     CDS             complement(3141..4094)
                     /locus_tag="WGR19_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799240.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAETYKKQYKLSCQNILYGYPNFRHASKGCHGER
                     IYFIDQVKIPFKKEERIYTLKKLITLAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            4477..5496
                     /gene="galE"
                     /locus_tag="WGR19_001755"
     CDS             4477..5496
                     /gene="galE"
                     /locus_tag="WGR19_001755"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0799241.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
     gene            5612..>6078
                     /locus_tag="WGR19_001756"
     CDS             5612..>6078
                     /locus_tag="WGR19_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0799242.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYWQQMPSEQHEAFRFHHISTDEVYGDLSGTDDLFTETTPYAP"
BASE COUNT         1949 a         1198 c         1077 g         1854 t
ORIGIN      
        1 attcttaatc acaaggaaag ctaaatgcaa aacaacaacg aatttaaaat tggtaatcgt
       61 tcagtaggtt acaaccacga accattgatt atctgtgaaa tcggcatcaa tcatgaaggc
      121 tctttaaaaa cagcttttga aatggttgat gctgcctata atgcaggcgc tgaagttgtt
      181 aaacatcaaa cacacatcgt tgaagacgaa atgtctgatg aggccaaaca agtcattcca
      241 ggcaatgcag atgtctctat ttatgaaatt atggaacgtt gcgccctgaa tgaagaagat
      301 gagattaaat taaaagaata cgtagagagt aagggtatga tttttatcag tactcctttc
      361 tctcgtgcag ctgctttacg attacaacgt atggatattc cagcatataa aatcggctct
      421 ggcgaatgta ataactaccc attaattaaa ctggtggcct cttttggtaa gcctattatt
      481 ctctctaccg gcatgaattc tattgaaagc atcaaaaagt cggtagaaat tattcgagaa
      541 acaggggtac cttatgcttt gcttcactgt accaacatct acccaacccc ttacgaagat
      601 gttcgattgg gtggtatgaa cgatttatct gaagcctttc cagacgcaat cattggcctg
      661 tctgaccata ccttagataa ctatgcttgc ttaggagcag tagctttagg cggttcgatt
      721 ttagagcgtc actttactga ccgcatggat cgcccaggtc cggatattgt atgctctatg
      781 aatccggata cttttaaaga gctcaagcaa ggcgctcatg ctttaaaatt ggcacgcggc
      841 ggcaaaaaag acacgattat cgcgggagaa aagccaacta aagatttcgc ctttgcatct
      901 gtcgtagcag ataaagacat taaaaaagga gaactgttgt ccggagataa cctatgggtt
      961 aaacgcccag gcaatggaga cttcagcgtc aacgaatatg aaacattatt tggtaaggtc
     1021 gctgcttgca atattcgcaa aggtgctcaa atcaaaaaaa ctgatattga ataatgctta
     1081 ttaacttagt tactttatta acagaggatt ggctattaca tatagctaat tctcattaat
     1141 ttttaagaga tacaataatg ctaaagaaaa taaaaaaagc tctttttcag cctaaaaagt
     1201 tttttcaaga ttcaatgtgg ttgacaacat ctccatttta tcttaccccc ccacgtaaca
     1261 atttatttgt catatctaat ttaggtcagc ttaaccaagt ccaaagccta attaaaatac
     1321 aaaaattaac caataattta ctagtaattt tatatacttc taaaaactta aaaatgccta
     1381 agttagttca tcaatcagct aacaagaatc tatttgaatc tatttatcta tttgagcttc
     1441 ctagaagccc taataatata actcctaaaa aattacttta tatttataga agttacaaaa
     1501 aaatccttaa tattatacag cctgctcatc tctatatgct gtcttttaca ggccactact
     1561 cctatctgat tagtattgca aaaaaaaaga atattacgac tcatttaatt gatgaaggga
     1621 ctggaacata tgctccttta ttagaatcat tttcatatca tccaacaaaa ttagaacgtt
     1681 atttgattgg aaataatctt aatattaaag gatatataga tcattttgac atattgcatg
     1741 tcccctttcc tgaatatgct aaaaaaatat ttaatgcaaa aaaatataac cggttttttg
     1801 cgcatgctgg aggaataagc attaataata acattgcaaa cttacagaaa aaatatcaaa
     1861 tatctaaaaa tgactatatt tttgttagtc aacgctaccc catttcagat gatttgtatt
     1921 ataagagtat agtagaaatc ttaaacagca taagtttaca aattaaagga aagatattta
     1981 ttaaactaca cccaaaagag atgggcaaca actatgtaat gtctttattt ctaaatatgg
     2041 tagaaataaa ccctcggctg gtagttatta atgaacctcc ttttctaatt gagcccctaa
     2101 tatacttaac aaatcctaaa ggaattatag gcctggcctc tagttcttta atttatacac
     2161 cattactctc accctcaacc caatgtcttt ctattggaga gttaattatt aacttaattc
     2221 aaaaatattc aatggtggaa aacactgaaa tgatccaaga acacttagag attattaaga
     2281 aatttaattt tattaatata ctaaatgatt taaatggggt aataagtaac cctctcttta
     2341 aaacagaaga aacatttgaa acacttctta aatctgcaga attcgcatat aaatctaaaa
     2401 actactttca ggctattttt tactggcaac ttgccagcaa aaacaatatt accttattag
     2461 ggcataaagc attatggtac tacaatgcac tttataatgt aaaacaaatt tataagatgg
     2521 aatattcaga tattttttat atcgataata tctccgtaga ctttcatagt aaagataaat
     2581 tgacatggga aaaaattaaa cattattact attccgccga caatagaatt ggtagagata
     2641 gataaaaata ctatgacttc tgatctccat acgtatgctt gttttcaaaa agcagcatta
     2701 agatagataa gtgaagcttg atgagagcca tttttgtggt catcgccaag gtacacgcgg
     2761 agagtaatcg acaacagagc tgctgtattc tggcttttaa agcccagtag taaagtttat
     2821 aaggctgcat ttctcaatgt aaaacgcgat attttcaccc attattaggg ataagtagcc
     2881 agtggtttac actggctact ttcatagtta tgaagtactg ggtctgagtg aattcaatta
     2941 tttccgtata aaacacagta gacattttac taatcggcaa aattatatta gaagaattga
     3001 gaactactga agtcaggtaa gataccattt tgcacaaatt taacggcatc cctaaaaagc
     3061 attttgggtg gtatttaaag gagtacaaaa tggttttaac tacagtatgc ttagaaatct
     3121 atagccttac gcgctttata ttaaatcacc tttctcaacc atttagaatc ataaaaaaac
     3181 tttttaggat ccctaattaa ctttttaaat tttcttaaaa aaatagtttt agttgattct
     3241 atactattca ccgcagataa atattcttgg taatcatgtt gcaatggcac aacctgcttt
     3301 aatagcttat taaactcatc agttgtaatc acctttttag tagcatagca atcaagccaa
     3361 tccggatctg ctatttttgg ggaagtgaaa tctatttgat caaaataaac taaaacccca
     3421 ctacctaaaa aatgctgatt tgcataggat ttagaaaagc ctaagtctgc aacaacacca
     3481 acagggatac ccttacattc agcttcaaaa agcatagtag atgaataaga taaacaatac
     3541 cccatttctt ggaaggcttg cgcggtactt tttcgctcta ttgtcaaatt actcggcaac
     3601 tgaaactgct ttgccagctc tatatacgaa tgtttatcct gatgcacagt aatatcttta
     3661 tctgcaaccc ttagcaaaat agtaaattct ttctcagggt atttttcagc caaggtaatc
     3721 aattttttta atgtgtaaat tctttcttct tttttaaatg gaattttgac ttggtcaata
     3781 aaataaattc tctctccatg acaacctttc gaagcatggc gaaaatttgg ataaccataa
     3841 agtatatttt gacaacttaa tttatattgt tttttatatg tctctgcaat tctaaaatca
     3901 tgcttattat tatataaaac aatatcagcc cccatacgag atagaatact tgcctgatca
     3961 ccgaatacta cacctggaaa caaggtaata atcaatggcc ttgaagcaat atttaattgc
     4021 gcattctgct taaagaacct ttttaacaac ccatttccaa ccgataaaat tactgcgtca
     4081 taatattgat gcatattctt aataaagtaa tcatcaatat gaaaaaaaaa attgcttggt
     4141 ctttatctat ccctgattct aataactgtc gatttgaaag aatgttttct cgacttttgt
     4201 ggatataaat atcaatttga gcatctttta tctcttttgc aacagcatag cccgagttaa
     4261 ggaacgagtc ataactggca attagtaata cttttttcac tattttccct taattctaca
     4321 ccatatgaat aaaattatag tattttacct tagatatcaa atatacttaa ccaaaaaaat
     4381 ctgaagaaat ctactgctac ttcagggata attatttcat taagatttta caaccattat
     4441 tatcaatcta tcaaagtatt tttagtattt tattctatga aaaaaattct cgttaccggc
     4501 ggcaccggtt ttatcggctc gcataccgtt gtttctttgc tgaaaagcgg ccatcaagtc
     4561 gtgattttgg ataacctatg caattccagc atcaatatcc tgccacgctt gaaaacgatt
     4621 accggccaag agattccgtt ttatcaaggc gatatccgcg atcgtgagat tttgcgccgt
     4681 atttttgcgg aaaaccgcat tgattcggtg attcattttg ccggcttgaa agcggtgggt
     4741 gaaagtgtgg ccgagccgat gaaatattat gataataatg tttccggcag cttggtgttg
     4801 gcggaagaaa tggcgcgtgc gggcgtgttt agcattgtgt tcagttcttc ggcgacggtt
     4861 tatggcgatc cgggcaaagt gccttatacc gaggatatgc cactgggcga taccaccagc
     4921 ccttacggca catcgaaatc aatggttgag cgcattctca ctgacattca aaaagccgat
     4981 ccgcgctgga gcatgatttt gttgcgttat ttcaatccaa ttggcgcgca tgaaagcggc
     5041 ttgattggcg agcagccaaa cggtatccca aataatttgt tgccttatat ctgccaagtg
     5101 gcggcaggca aactgccgca attagcggta ttcggcgatg actaccctac ccctgatggc
     5161 acgggtatgc gcgactatat tcatgtgatg gacttggctg aaggccatgt cgccgccatg
     5221 caggcgaaaa gcaatgtagc aggcacgcat ttgttgaact taggctccgg ccgcgcttct
     5281 tcggtgttgg aaatcatccg cgcatttgaa gcagcttcgg gtttgacgat tccgtatgaa
     5341 gtcaaaccgc gccgtgccgg tgatttggcg tgcttctatg ccgacccttc ctatacaaag
     5401 gcgcaaatcg gctggcaaac ccagcgtgat ttaacccaaa tgatggaaga ctcatggcgc
     5461 tgggtgagta ataatccgaa tggctacgac gattaagttg acctgataca ggccgtctga
     5521 aagagatgtt ttcagacggc ctctttatct gaaaaacaca cattctgtct gctataatct
     5581 gtttatattt tttggctatc ctctgaaatt tatgagaaaa atcctcgtta ccggcggcgc
     5641 gggctttatc ggttctgccg ttgtccgtca tattatccga aacacccaag attccgtcgt
     5701 caacctcgac aaactgacct acgcgggcaa tctcgaatcg ctgaccgaca ttgccgataa
     5761 tccccgctat gctttcgagc aagtggacat ttgcgaccgc gccgaactcg accgcgtatt
     5821 cgcacaacac cggcccgatg ccgtgatgca cttggcggcg gaaagccatg tcgaccgctc
     5881 tatcggttcg gcaggcgagt ttatccaaac caatatcgtc ggcacattca atctgcttga
     5941 agcagcccgc gcctactggc aacaaatgcc gtctgaacag cacgaagcct tccgtttcca
     6001 ccatatttcc accgatgaag tctatggcga tttaagcggc acagacgatt tgtttaccga
     6061 aaccacgccc tacgcgcc
//
LOCUS       DAWFJW010000112         5912 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFJW010000112 DAWFJW010000000
VERSION     DAWFJW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.112"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..434)
                     /locus_tag="WGR19_001757"
     CDS             complement(<1..434)
                     /locus_tag="WGR19_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ0799243.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGAPAPKGPA"
     gene            complement(470..583)
                     /gene="rrf"
                     /locus_tag="WGR19_001758"
     rRNA            complement(470..583)
                     /gene="rrf"
                     /locus_tag="WGR19_001758"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(680..3571)
                     /locus_tag="WGR19_001759"
     rRNA            complement(680..3571)
                     /locus_tag="WGR19_001759"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3978..4053)
                     /locus_tag="WGR19_001760"
     tRNA            complement(3978..4053)
                     /locus_tag="WGR19_001760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4018..4020),aa:Ala,seq:tgc)
     gene            complement(4059..4135)
                     /locus_tag="WGR19_001761"
     tRNA            complement(4059..4135)
                     /locus_tag="WGR19_001761"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4099..4101),aa:Ile,seq:gat)
     gene            complement(4237..5777)
                     /locus_tag="WGR19_001762"
     rRNA            complement(4237..5777)
                     /locus_tag="WGR19_001762"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1273 a         1733 c         1283 g         1623 t
ORIGIN      
        1 gctgggcctt ttggtgcggg cgcgccgacg gaagcctgat ccttcagctt cgccagcacc
       61 gcagggccga tgccctttac cttggtcaaa tcgtccacag acttgaacgc accgttttgc
      121 gcacggtatt ccgcaatggc cttcgccttc gccggaccta tgcccggcag cgcctccaac
      181 tcctgctgcg aagccgcatt gatgtttacc gccgcaaggg agaaggcgca ggagaacagc
      241 atacagaaca gcacgaacat tttcttcatg gtttttcctt taagggttgc aaacaataaa
      301 ccgcatcttg cgacgataaa acgggtcatt ctaaaatgaa tatcccaaag tttcaagccg
      361 ttcctccgca aacccgaccg gacgccgtac ggatactgtc ccgccatccc cgacattttt
      421 tccgggcaaa gcaaaaaccc ccgaataatc gggggttttc tgaatgggtg tttggcagtg
      481 acctactttc gcatggaaga accacactat catcggcgct gagtcgtttc acggtcctgt
      541 tcgggatggg aaggcgtggg accaactcgc tatggccgcc aaacttaaac tgttacaaat
      601 cggtaaagcc ttaatcaata tattcggtaa tgactgaatc agtcagtaag cttttatctc
      661 ttgaagttct tcaaatgata gagtcaagcc tcacgagcaa ttagtatggg ttagcttcac
      721 gcgttaccgc gcttccacac cccacctatc aacgtcctgg tctcgaacga ctctttagtg
      781 cggttaaacc gcaagggaag tctcatcttc aggcgagttt cgcgcttaga tgctttcagc
      841 gcttatctct tccgaactta gctacccggc tatgcaactg gcgttacaac cggtacacca
      901 gaggttcgtc cactccggtc ctctcgtact aggagcagcc cccgtcaaac ttccagcgcc
      961 cactgcagat agggaccaaa ctgtctcacg acgttttaaa cccagctcac gtaccacttt
     1021 aaatggcgaa cagccatacc cttgggaccg actacagccc caggatgtga tgagccgaca
     1081 tcgaggtgcc aaactccgcc gtcgatatga actcttgggc ggaatcagcc tgttatcccc
     1141 ggagtacctt ttatccgttg agcgatggcc cttccataca gaaccaccgg atcactatgt
     1201 cctgctttcg cacctgctcg acttgtcggt ctcgcagtta agctaccttt tgccattgca
     1261 ctatcagtcc gatttccgac cggacctagg taaccttcga actcctccgt tacgctttgg
     1321 gaggagaccg ccccagtcaa actgcctacc atgcacggtc cccgacccgg atgacgggtc
     1381 tgggttagaa cctcaaagac actagggtgg tatttcaagg acggctccgc agagactggc
     1441 gtctctgctt ctaagcctcc cacctatcct acacaagtga cttcaaagtc caatgcaaag
     1501 ctacagtaaa ggttcacggg gtctttccgt ctagcagcgg gtagattgca tcttcacaac
     1561 cacttcaact tcgctgagtc tcaggaggag acagtgtggc catcgttacg ccattcgtgc
     1621 gggtcggaac ttacccgaca aggaatttcg ctaccttagg accgttatag ttacggccgc
     1681 cgtttactgg ggcttcgatc cgatgctctc acatcttcaa ttaaccttcc agcaccgggc
     1741 aggcgtcaca ccctatacgt ccactttcgt gttagcagag tgctgtgttt ttaataaaca
     1801 gtcgcagcca cctattctct gcgaccctcc ggggcttacg gagcaagtcc ttaaccttag
     1861 agggcatacc ttctcccgaa gttacggtat caatttgccg agttccttct cctgagttct
     1921 ctcaagcgcc ttagaattct catcctgccc acctgtgtcg gtttgcggta cggttcgatt
     1981 caaactgaag cttagtggct tttcctggaa gcgtggtatc ggttgcttcg tgtccgtaga
     2041 cactcgtcgt cacttctcgg tgttaagaag acccggattt gcctaagtct tccacctacc
     2101 ggcttaaaca agctattcca acagcttgcc aacctaacct tctccgtccc cacatcgcat
     2161 ttgaatcaag tacaggaata ttaacctgtt tcccatcgac tacgcatttc tgcctcgcct
     2221 taggggccga ctcaccctac gccgatgaac gttgcgcagg aaaccttggg ctttcggcga
     2281 gcgggctttt cacccgcttt atcgctactc atgtcaacat tcgcacttct gatacctcca
     2341 gcacacttta caatgcacct tcatcggcct acagaacgct cccctaccat gccggtaaac
     2401 cggcatccgc agcttcggtt atagatttga gccccgttac atcttccgcg caggacgact
     2461 cgaccagtga gctattacgc tttctttaaa tgatggctgc ttctaagcca acatcctggc
     2521 tgtctgggcc ttcccacttc gtttaccact taatctatca tttgggacct tagctggcgg
     2581 tctgggttgt ttccctcttg acaacggacg ttagcacccg ctgtctgtct cccgaggaac
     2641 cacttgatgg tattctgagt ttgccatggg ttggtaagtt gcaataaccc cctagccata
     2701 acagtgcttt acccccatca gtgtcttgct cgaggcacta cctaaatagt tttcggggag
     2761 aaccagctat ctccgagttt gtttagcctt tcacccctat ccacagctca tccccgcatt
     2821 ttgcaacatg cgtgggttcg gtcctccagt acctgttacg gcaccttcaa cctggccatg
     2881 gatagatcac tcggtttcgg gtctacaccc agcaactcat cgccctatta agactcggtt
     2941 tccctacgca tcccctattc ggttaagctc gctactgaat gtaagtcgtt gacccattat
     3001 acaaaaggta cgcagtcaca cctctagggc gctcccactg tttgtatgca tcaggtttca
     3061 ggttctattt cactcccctc ccggggttct tttcgccttt ccctcacggt actggttcac
     3121 tatcggtcga tgatgagtat ttagccttgg aggatggtcc ccccatattc agacaggatt
     3181 tcacgtgtcc cgccctactt ttcgtacgct tagtaccgct gttgagattt cgaatacggg
     3241 actgtcaccc actatggtca agcttcccag cttgttcttc tatctcgaca gttattacgt
     3301 acaggctcct ccgcgttcgc tcgccactac ttgcggaatc tcggttgatt tcttttcctc
     3361 cgggtactta gatggttcag ttctccgggt tcgcttctct aagtctatgt attcaactta
     3421 ggatactgca cagaatgcag tgggtttccc cattcggaca tcgcgggatc attgctttat
     3481 tgccagctcc cccgcgcttt tcgcaggctt acacgtcctt cgtcgcctat catcgccaag
     3541 gcatccgcct gatgcactta ttcacttgac tctatcattt caagaacttc tttgactttg
     3601 cctaacattc cgttgactag aacatcagac ttgaatttcc tactttgata aagcttactg
     3661 ctttgttgtg tcttaatcct gccttttgtg tttcaggatt aagtcgatac aatcatcacc
     3721 caaatactgt gtttgttttc ttttctcttg tgagcgattt tttatccttt gcaaagaata
     3781 aaaaatcaaa acaaacgctt tgtctttgtt tgttgatttc ggctttccaa tttgttaaag
     3841 atcgatgcgt tcgatattgc tatctactgt gcaaatcaaa acgagctgat tattatatca
     3901 gcattttgtt cttggtcaag tgtgacgtcg ccctgaatgg attctgttcc attcttccgt
     3961 tttgatttgt acagtattgg tggaggcaaa cgggatcgaa ccgatgaccc cctgcttgca
     4021 aagcaggtgc tctaccaact gagctatgcc cccgttcttg gtgggtctgg gaggacttga
     4081 acctccgacc ccacgcttat caagcgtgtg ctctaaccag ctgagctaca aacccggatt
     4141 ctcttcttaa gcgaatcttg ccttcactca agcttcttcc gcatcttttt cagtttaccg
     4201 ataagtgtga atgcctaaag cctcttcttt ctctagaaag gaggtgatcc agccgcaggt
     4261 tcccctacgg ctaccttgtt acgacttcac cccagtcatg aagcataccg tggtaagcgg
     4321 gctccttgcg gttaccctac ctacttctgg tatcccccac tcccatggtg tgacgggcgg
     4381 tgtgtacaag acccgggaac gtattcaccg cagtatgctg acctgcgatt actagcgatt
     4441 ccgacttcat gcactcgagt tgcagagtgc aatccggact acgatcggtt ttgtgagatt
     4501 ggctccgcct cgcggcttgg ctaccctctg taccgaccat tgtatgacgt gtgaagccct
     4561 ggtcataagg gccatgagga cttgacgtca tccccacctt cctccggctt gtcaccggca
     4621 gtctcattag agtgcccaac tgaatgatgg caactaatga caagggttgc gctcgttgcg
     4681 ggacttaacc caacatctca cgacacgagc tgacgacagc catgcagcac ctgtgttacg
     4741 gctcccgaag gcactcctcc gtctccagag gattccgtac atgtcaagac caggtaaggt
     4801 tcttcgcgtt gcatcgaatt aatccacatc atccaccgct tgtgcgggtc cccgtcaatt
     4861 cctttgagtt ttaatcttgc gaccgtactc cccaggcggt caatttcacg cgttagctac
     4921 gctactaagc aatcaagttg cccaacagct aattgacatc gtttagggcg tggactacca
     4981 gggtatctaa tcctgtttgc tacccacgct ttcgggcatg aacgtcagtg ttgtcccagg
     5041 aggctgcctt cgccatcggt attcctccac atctctacgc atttcactgc tacacgtgga
     5101 attctacctc cctctgacac actcgagtca cccagttcag aacgcagttc ccgggttgag
     5161 cccggggatt tcacatcctg cttaagtaac cgtctgcgcc cgctttacgc ccagtaattc
     5221 cgattaacgc tcgcacccta cgtattaccg cggctgctgg cacgtagtta gccggtgctt
     5281 attcttcagg taccgtcatc agccgctgat attagcaaca gccttttctt ccctgacaaa
     5341 agtcctttac aacccgaagg ccttcttcag acacgcggca tggctggatc aggcttgcgc
     5401 ccattgtcca aaattcccca ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc
     5461 agtgtggcgg atcatcctct cagacccgct actgatcgtc gccttggtag gcctttaccc
     5521 caccaaccag ctaatcagat atcggccgct cgaatagcgc aaggcccaaa ggtcccctgc
     5581 tttctctctc aagacgtatg cggtattagc tgatctttcg atcagttatc ccccactact
     5641 cggtacgttc cgatatgtta ctcacccgtt cgccactcgc cacccgagaa gcaagcttct
     5701 ctgtgctgcc gtccgacttg catgtgtaaa gcatgccgcc agcgttcaat ctgagccagg
     5761 atcaaactct tatgttcaat ctctaacttt ttaacttctg gtctgcttca aagaaaccaa
     5821 caggacaatg ttcaaaacat tatcttgtct gtctttcaaa cagtgtgagg ctcaaggcac
     5881 tcacacttat cggtaatctg ttttgttaaa ga
//
LOCUS       DAWFJW010000113         5700 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFJW010000113 DAWFJW010000000
VERSION     DAWFJW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.113"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(196..3027)
                     /gene="lbpA"
                     /locus_tag="WGR19_001763"
                     /gene_synonym="tbpA"
     CDS             complement(196..3027)
                     /gene="lbpA"
                     /locus_tag="WGR19_001763"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0799244.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLILDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYGIKKPSEGGEYFLAEGESEL
                     KPVAKVAGNGNYLNNQLNRWVKERIEKNQPLNAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDQKLIRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISHPEQLSHNAARISEYS
                     NYTDKGDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSTGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3024..5222)
                     /locus_tag="WGR19_001764"
     CDS             complement(3024..5222)
                     /locus_tag="WGR19_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0799245.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSIETTPVNQPAVGAAMRLLRRNTAFHREDGTAIPNSKQAEEELSFKEE
                     DILFLYGSKKDQRKQLEDKIHQRNPNVKIRTSENENGKDSYKFVDAGYVYAKGEDEIK
                     RISNYRQISNRLGYDGFVYYSGEHPSQSLPSAGMVEYSGNWQYMTDAKRRRTGQAVAS
                     EDLGYTTYYGNEIGATSYAARDADDRERHPAKYTVDFYKKTLTGQLIKNQYVQNKSNL
                     NEPKNPLTIYNITAKLDGNRFTGSASVNPDLATNHANKEHLFFHTNADQRLEGGFFGD
                     KGEELAGRFISNDNSVFGVFAGKQKTEAANAADTKPALPSGKHTKILDSLKISVDEAT
                     DGHARKFAISSMPDFGHPDKLLVEGREIPLVNEEQIIKLADGRKMTVSACCDFLTYVK
                     LGRIKTERPAVKPKAQDEEDSDIDNGEESEDEISEDDNGEDEVTEEEEAEETEEETDE
                     DEEEEPEETEEKSPTEGNGGSGSILPALEAPKGRDIDLFLKGIRTAEADIPQTGKAHY
                     TGTWEARISKPIQWDNKADKAAKAEFNVDFGEKSISGTLTEKNGVEAAFYIEKGVIEG
                     NGFHATARTRENGIDLSGQGSTRSQSFKADNLLVTGGFYGPKAEELGGTIFNNDGKSL
                     SITENIENEAEAEAEVEVEADVGKQLEPDEVKHKFGVVFGAKKDMQEVEK"
BASE COUNT         1241 a         1542 c         1294 g         1623 t
ORIGIN      
        1 aaagtgggaa tccagttttt cgagtttcag tcattcccga taaattgcct tagcattgaa
       61 tgtctagatt cccgcctgcg cgggaatgac agcggaaatc ttgtttatat cgaatcaaaa
      121 aaacctgcac cttaatcagt tggcggttta gtccgacttt tggggtgcag atcaagcttt
      181 cagacggtat ttcctttaaa acttcatttc gagcgcgagg ctgaagttcc tgcccggtgc
      241 ggcatacctt ccatagttgc tgtcgccgcc gtgccggttt gccgtgcttt ccgcggtctg
      301 gcgcaaggat tcccaagtaa cgtagcggta gttgccgata ttgtagatag ccgccctcaa
      361 ggtcagccgt tttttcagat tcagataggc ggaaacgtct gccgtcgacc aagaagacga
      421 cgctcttttt gtcgaatatc gtttttgatc gcctgccaga taagcaagct cgtcagggtt
      481 tttccctttg gaataggtca gcataatgtt tgcgccccat ttcccctcgg gctggtcgta
      541 tccgaacccc aaaacataac gcgacggctg taccgcatcc aaagcatagc tgcggaggga
      601 cagtcccggc cggttggata ccgatttcgg tttgatgcgg ttgtacgcca aagtcgtgta
      661 caaaccttcg ggcagttttc cgtacacgcc gttccagtcg atttttccca atatattaat
      721 gccttggagc gacatatttt gggcattgta ataatcgcgt atatcaatct ctgtcaatcg
      781 tcctgttgaa tccggcaatt tggttttttg atcggcaacg gcaatcatat cggtatagcg
      841 gttgcggaag ctgctgattt ccaaaaagcc gaaatcgccc ttccactgca aaccgatttc
      901 tcggttggct gccttttccg atttcagggc gggacgctgc cagcctttcg gataatcgtg
      961 ataaatgtct atcccgaaaa gttcttggaa ggaaggcgtt ctgaagccgc tggaggcgcg
     1021 gtaagacacg gaaaaatgcc ggttcggttt gaacacgatg ccgctgttcc acgaacggtc
     1081 aacataccgc ccgctgcgga cgagttcttc cgacgtggtg aagtttttcc ggtcgtacct
     1141 gccgcccaag ctgaaatcga aatatttgcc gattgaaaaa cggtcgttca aagaaatgtg
     1201 gatattgctg ccgttgattt ttcttggcac gcatttgcgg gaacgcaggg tttcgatgta
     1261 gccgcagacc gacccttcga cgacttcggg cttacccaaa agatacttgt ctcccttatc
     1321 ggtgtaattc gagtattccg aaatccttgc cgcattgtga gaaagctgtt cggggtggga
     1381 aatcgcgttg gaagcatcgt aaccgaagcc caaagtcaga tggtgtttcg tccatttgtt
     1441 tttcagcgat ttctcaaacg aggcattcaa aacattgtgc tgttcgcggt agtggaaacg
     1501 gtcgctgctg tcgtaggaat acggtttgtc cgccgacgcg cggcaggatt tgtccacggc
     1561 aggatacacg gcgcaattca gcttcagcgt gttgttgtcg gttgccacgc cctgtttgtc
     1621 aaacgacaac accgccttat ccgcccagtt atccgagtat ttctcatttt cataacggta
     1681 cagcaaaccc atacggcggc ggcggtgatg ttcgtcaata aatttggtgc gggaatattt
     1741 caaacctatg cccctaatca acttttgatc gcccttccac tcttctatat tcggcacaaa
     1801 atacaagccg tcgcggaaat cgtcgccgtc gtacaccccg ctcttgtctc taaacttttt
     1861 cgcctcgtcc gtaccgtaat actgtttttc cgtcatatcg cggatatcgt aacgctgttt
     1921 ggtatcctca aacacgccgc cgacataatg cctgccgccg aagcggtagc ccagcttggc
     1981 aagccaagag ccgctgcggt aatccatcgg atcgggcaat atcctgccgc cgcccgtgta
     2041 agcttgggcg gacaggtttt cgtggcgcgc ctgcgcctcc cgcaccatcg cttcttcttc
     2101 agcatttaaa ggctgatttt tttcaatacg ttcttttacc cagcggttga gctggttgtt
     2161 caaatagttt ccgttgcctg ccacttttgc cacaggcttg agctcactct cgccctccgc
     2221 caggaaatat tcccctcctt cacttggttt tttaatgccg tatgtctggc ggaacgcgtc
     2281 caaacggttt atgccgtatg ccaccccgtc cgcaatatcg ccgtgcgggc gcgtttcccg
     2341 cccttgacgt tcggttcgga ttagcagccc ttcccaaccg tccttactga atcccgcacc
     2401 gagcgacttc ataaattggc ggtttttact gccgtaggcg gtttttgcct gtatccccca
     2461 acttttgccg tctaaaatca ggtctgccgc ctctttggtg cggaaggcga ccgcgccgcc
     2521 gagtgcgccg ctgccgtgat cggacgaacc ggcacctttg tcgatttcca ccgtgctgat
     2581 gttttcatat tcgatttcgt tgattgcacc gctgccgccg cgtccgccgt atccgctcaa
     2641 cgatccctgc acggtaaacg cctgtatttg ggcaacgccg tcaaccgaaa ccgccacacg
     2701 gtttttatcc acgccgcgta tcgagtagcc gccgctcgcg ccgttgccct gttcgacaac
     2761 cgccacgccc ggatcgtagc gcgtcaggtc gcggataccg agtacctgtt ctttgttcaa
     2821 cgtttccgac gttttgacga ttttgcccaa accggtcgcc tctttcgatc gccgtcccac
     2881 tttggcggca cggacggtaa cctctttcag ggattgggtc tgcgcggcat caggtgtcgc
     2941 cgtttcaggg tttgccgcat atgacgggaa agcggcggca acagccaagg cagtcagggt
     3001 aagctgaaaa ccgtgtttct tattcatttt tccacctcct gcatatcttt cttcgcaccg
     3061 aataccacgc cgaatttgtg tttaacttca tcaggttcta actgtttgcc aacatcagct
     3121 tcaacttcaa cttcagcttc agcttcagct tcattttcaa tattttcagt tatactaaga
     3181 gatttcccat cattattgaa aatagtaccg cccaattcct ccgccttcgg gccgtaaaag
     3241 ccgcccgtta caagaagatt gtcggctttg aagctctgcg atctggtcga accctgcccg
     3301 gaaaggtcga tgccgttctc ccgagtgcgc gctgtcgcgt ggaaaccgtt gccctcaatc
     3361 acaccttttt caatatagaa agcagcttct acaccatttt tctccgtcag cgttccggaa
     3421 atcgatttct cgccgaaatc aacattaaac tctgcttttg ccgctttatc cgccttatta
     3481 tcccattgaa tgggtttgct gatacgcgct tcccaagtgc cggtatagtg tgcttttccg
     3541 gtttgcggaa tgtcggcttc cgccgtgcgg atacctttca ggaaaaggtc gatgtccctg
     3601 cctttagggg cttctagggc aggcaggatg ctgcctgaac cgccgttgcc ttctgtcggc
     3661 gatttttctt cagtttcttc gggttcttcc tcttcgtctt catcagtttc ttcttcggtt
     3721 tcttcagctt cctcttcttc ggtgacttca tcttcgccgt tatcatcttc ggaaatttcg
     3781 tcttcgcttt cttcgccatt atcaatgtcc gaatcctctt catcctgcgc cttcggtttg
     3841 acggcggggc gttcggtttt tatccgtccg agtttcacat aggtcagaaa atcgcaacaa
     3901 gcactgacgg tcattttcct gccgtcggca agcttgatga tttgttcttc gtttaccaaa
     3961 ggaatttcac gcccttcgac aagaagtttg tcgggatgac caaaatcggg catagaggaa
     4021 atggcaaact tacgggcatg gccatcagtc gcctcgtcaa cggaaatttt tagagaatcc
     4081 aagattttgg tgtgttttcc agacggcagg gcaggttttg tatctgctgc gtttgctgcc
     4141 tctgtttttt gtttgcctgc gaatacgccg aatacgctgt tgtcgttgct gataaaccgt
     4201 ccggcaagct cttccccctt atcgccgaaa aaaccgccct caagccgctg atcagcattg
     4261 gtatggaaaa acaaatgctc cttattggca tggtttgtcg ctaaatcagg attgacactg
     4321 gcactgccgg taaagcggtt gccgtccaat tttgcagtaa tgttgtaaat ggtcagtggg
     4381 tttttgggct catttagatt acttttattt tgcacatact gatttttaat taattgaccc
     4441 gtcaaagttt tcttataaaa atcaaccgta tatttggcag gatgcctttc tcggtcatcg
     4501 gcatccctag ccgcataaga agttgcacca atctcattac cataatatgt ggtataaccc
     4561 aaatcctcac tggcaaccgc ctgacctgtc cgacgacgtt tggcatcggt catatattgc
     4621 cagttaccgg aatattccac cattcccgcg ctcggtaaag attgggaagg atgttctccg
     4681 gaataatata caaaaccgtc atagcctaaa cgattagaaa tctgcctgta atttgaaatc
     4741 cgcttaattt catcttctcc ctttgcatat acataacctg catctacaaa tttataacta
     4801 tcttttccat tttcattttc tgatgtccta atttttacat taggattgcg ttgatgaatt
     4861 ttatcttcaa gctgcttacg ttgatctttt tttgaaccgt ataaaaacag gatatcttcc
     4921 tctttaaacg acagctcttc ttctgcttgt ttgctatttg gaattgccgt gccatcttca
     4981 cgatgaaaag cagtattccg tctcaacagc cgcattgccg caccaacggc gggctggttg
     5041 accggcgtgg tttctataga aggcccggca ggggggggag tgggaacgtc cttagacttg
     5101 aaagtgacgg ggtacgcggt cggcgttgat tcgacaacag gctgcacgcc gaaattgccg
     5161 ccgatacaag atgctaaaag taagggcaac aagacaatgc cgccataatt cggtttacac
     5221 atccctactt ttcctcctat ttgattaata ataattatca ttatattaat atgtacagat
     5281 aatatcaagc cgtttttata gtgaattaac aaaaatccgg acaaggcgac gagccgcaga
     5341 cagtacagat acatcccgtc attcccacga acctacatcc cgtcattccc acgaacctac
     5401 atcccgtcat tcccacgaac ctacattccg tcattcccac gaacctgcac cacgtcattc
     5461 ccacgaacct acatcccgtc attcccacga acctgcacca cgtcattccc acgaaagtgg
     5521 gaatccagtt cgttcggttt cgcttgtttt aagtttcggg taacttccac ttcgtcattc
     5581 ccacgaacct acatcccgtc attcccacga aagtgggaat ccaggacgca aaatctcaag
     5641 aaaccgtttg acctgataag tttccgcgct gacagaccta gattcccgcc tgcgcgggaa
//
LOCUS       DAWFJW010000114         5614 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFJW010000114 DAWFJW010000000
VERSION     DAWFJW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.114"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..228
                     /locus_tag="WGR19_001765"
     CDS             <1..228
                     /locus_tag="WGR19_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0799246.1"
                     /translation="SEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADG
                     LAVALTHALRNHGLAAKLNPSGMQVKRGRFQ"
     gene            279..1148
                     /locus_tag="WGR19_001766"
     CDS             279..1148
                     /locus_tag="WGR19_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0799247.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            1898..2692
                     /locus_tag="WGR19_001767"
     CDS             1898..2692
                     /locus_tag="WGR19_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0799248.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSTQTEDKPHWSEFAQAWK"
     gene            2904..5507
                     /gene="pepN"
                     /locus_tag="WGR19_001768"
     CDS             2904..5507
                     /gene="pepN"
                     /locus_tag="WGR19_001768"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0799249.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPIMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSNDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDGLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
BASE COUNT         1406 a         1455 c         1478 g         1275 t
ORIGIN      
        1 tcggaataca cggccttgca ggtcaaacag gcggtagtcg gcaagggcaa ggcggcaaaa
       61 gaacaggtgc agcatatggt ggtgcagatg ctcggcttat cgggaacgcc gcaggcggat
      121 gcggcggatg gtcttgccgt cgcgctgacc cacgccttgc gcaaccatgg gcttgctgcc
      181 aaactcaatc cttcggggat gcaggtcaag cgcggcaggt ttcaatagtt tcagacggca
      241 tttgtatttt gccgtctgaa aagaaaatgt gtatcgagat gaaatttata ttttttgtac
      301 tgtatgtttt gcagtttctg ccgtttgcgc tgctgcacaa acttgccgat ctgacgggtt
      361 tgcttgccta ccttttggtc aaaccccgcc gccgtatcgg cgaaatcaat ttggcaaaat
      421 gctttcccga gtgggacgga aaaaagcgcg aaaccgtatt gaagcagcat ttcaaacata
      481 tggcgaaact aatgttggaa tacggtttat attggtacgc gcccgccggg cgtttgaaat
      541 cgctggtgcg ttaccgcaat aagcattatt tggacgacgc gctggcggcg ggggaaaaag
      601 tcatcatcct gtatccgcac tttaccgcgt tcgagatggc ggtgtacgcg cttaatcagg
      661 atgtaccgct gatcagtatg tattcccacc aaaaaaacaa gatattggac gcacagattt
      721 tgaaaggccg caaccgctac gacaatgtct tccttatcgg gcgcaccgaa gggctgcgcg
      781 ccctcgtcaa acagttccgc aaaagcagcg cgccgtttct gtatctgccc gatcaggatt
      841 tcggacgcaa cgattcggtt tttgtggatt ttttcggtat tcagacggca acgattaccg
      901 gcttgagccg cattgccgcg cttgcaaatg caaaagtgat acccgccatc cccgtccgcg
      961 aggcggacaa tacggttaca ttgcatttct acccggcttg ggaatccttt ccgagtgaag
     1021 atgcgcaggc cgacgcgcag cgcatgaacc gttttatcga ggaacgcgtg cgcgaacatc
     1081 ccgagcagta tttttggctg cacaagcgtt tcaaaacccg tccggaaggc agccccgatt
     1141 tttactgact acgtaaaatt acaaaacata tcaggcgttt caagtcgaaa ttcctgattg
     1201 tttttggaaa tttgaaatcc gggtcgtgca aacaggattt gccggacggt tttaacggtt
     1261 cagttgtttg taaaaacaat gctttttaag attgacaaaa aaccagaatc aggttttagg
     1321 gcttattccg cgaacaaagg gggcggatgc cgaaaatccg attaatatat taaaattaaa
     1381 tttgtttaaa aacataaagt tgtaaacaag tatctcatat aagccttttt cattaaacag
     1441 atagtcagat attttgtgct aaaaatttat ataatattta aattaatatc aagttataaa
     1501 aatatataga attttatttt gtttatttat aattttaagc aataatctaa atcaggcatt
     1561 ttgattcccc tgttttaaaa aatacttggc aaggtgtgaa aaaaggcgta cattccgcta
     1621 cacagaatta cagacgcagc ggagcaatgc cgtctgaaag gattttccgg tcagtcttgc
     1681 gattggtcgg ggtttcatcg gaaacggtga aacgaaagtt tgccggcgta gagttgagct
     1741 acgcgggcaa agccgcaggc gaaagcctgt attgtttgtg aagcgtaaat ctctgatttg
     1801 aggtattggg gcaatcctgt ggggggttgc ctcttttttt atccgccttt taatgacaca
     1861 ataggcgcaa gtctgttttt gaatgcaagg tactgccatg aatacgattt tggctttcga
     1921 tattgaaacc gtacccgatg tgcagggtat ccgaacattg tacgagctgc cgtcctcgct
     1981 gcccgacgat gaagtggtgc tgtttgccca gcaaaaacgc cgtgctcaga cgggtgggga
     2041 ttttatgcag catcatcttc atcagattgt ggcgatttcg tgctgcatgc gttgggggca
     2101 ggataaagtc catgtcggca ccatcggcga gatggacgat ggcgaagaag tcgtgattgc
     2161 caaatttttc gagttgattg aaaaacatac gccgcaactg gtcagttgga acggcggcgg
     2221 tttcgacctg ccggttctgc attaccgctc cctgatatac ggcattaacg ccgcgcgcta
     2281 ttgggatatg ggcgacggcg atttcggcga cagccgcgat ttcaagtgga acaactacat
     2341 cagccgttat caccaacgcc actgcgattt gatggatttg ctcgcgcttt accagccgag
     2401 ggcaaacgtg ccgctggacg atatggcgaa actgtgcggt tttccgggca agctgggtat
     2461 ggacggcagc aaggtgtggg atgcgttcca cgcgggcagg ctgaaagaaa tccgcaatta
     2521 ttgcgaaacc gatgccgtga atacgtattt gatgtatctg cgtttttgtc tggtcagcgg
     2581 cagattcgac gcggacgaat acgaaatgga aatcaagcgg atcagaaact atctttccac
     2641 ccaaacagaa gataaaccgc attggtcaga atttgcccaa gcgtggaaat agaaaacctg
     2701 ctgtccgaag gcggtacgct tgtgccgccg tttcggtttc agacggcata tggtggatta
     2761 aattcaaacc ggtacagccc gtactatctg tactgtctgt ggcttcgtcg ccttgtcctg
     2821 atttttgtta atccgcttta aaagctgtat ccgtgctgtc tgaagccttt tccagtcaaa
     2881 ccaaacaata ttgaaagaaa aaaatgagca aaaccgtccg ttatctgaaa gattaccaaa
     2941 cgcctgccta ccgcatcctt gaaaccgacc tgcattttga tattgccgaa ccgcaaactg
     3001 tcgtgaaatc ccgtttgacg gtcgagccgc agagggtggg ggagccgctg gtgttggacg
     3061 gttcggcgaa actcttgtcc gtcaaaatca acggggcggc ggcggattat gtgttggaag
     3121 gtgagacgct gacgattgca gacgtgccgt ccgaacgctt caccgtcgaa gtggaaaccg
     3181 aaatcctgcc ggcggaaaac aaatcgctga tggggctgta tgcgtccgct ggtaacctgt
     3241 ttacccagtg cgagccggag ggcttccgca aaatcacgtt ctatatcgac cgtccggatg
     3301 tcatgtccaa gttcacgacc accatcgtcg cggacaaaaa acgctatccc gttttgctct
     3361 ccaacggcaa caaaatcgac ggcggcgagt attcagacgg ccgccattgg gtgaaatggg
     3421 aagacccgtt tgccaaaccg agttatctgt ttgctttggt cgcgggcgat ttggcggtca
     3481 cggaagacta tttcaccacc atgagcggca gaaacgtcaa aatcgagttt tacaccaccg
     3541 aagcggacaa gcccaaggtc ggctttgccg tggaatcgct gaaaaacgca atgaagtggg
     3601 acgaaacgcg cttcggtttg gaatacgact tggatatttt catggtcgtc gccgtgggcg
     3661 atttcaatat gggcgcgatg gaaaacaagg ggctgaacat ttttaatacc aagttcgtcc
     3721 ttgccgacag ccgtaccgcc accgataccg attttgaagg catcgaatcc gtggtcggac
     3781 acgagtattt ccacaactgg acaggcaacc gcgttacctg ccgcgattgg ttccagcttt
     3841 cgctgaaaga agggttgacc gtgttccgcg accaagaatt ttccggcgac cgcgccagcc
     3901 gcgccgtgcg ccgcatcgag aacatccgcc tgctgcgcca gcaccagttc cccgaagacg
     3961 caggcccgac cgcccatccg gtgcgccccg ccagctatga ggagatgaac aatttctaca
     4021 ccatgaccgt ttatgaaaaa ggcgcggaag tggtgcggat gtatcacacc ttgctcggcg
     4081 aagagggctt ccaaaaaggc atgaagctct atttccaacg acacgacggg caggccgtga
     4141 cctgcgacga tttccgcgcg gcgatggcgg acgcgaacgg catcaatctc gaccagttcg
     4201 ccttgtggta cagccaggcg ggtacgcccg tcttggaagc ggaaggtcgt ctgaaaaaca
     4261 atagtttcga gttaaccatc aaacaaaccg tgccgcccac gcccgatatg gcggacaagc
     4321 agccgataat gattcccgtc aaagtcgggc tgctgaaccg caacggcgaa gcggtggcat
     4381 tcgattatca gggcaaacgc gcaaccgaag ccgtgttact gctgaccgaa gccgaacaga
     4441 ccttccagtt cgaaagcgta accgaagccg tcgttccctc gctgctgcgc gggttcagcg
     4501 cgccagtgca tctgaactat ccgtacagca acgacgacct gctgctcctg ctcgcccacg
     4561 acagcgacgc cttcacgcgc tgggaagccg cccaaacgct ctaccgccgt gccgtcgccg
     4621 ccaaccttgc cgcactttca gacggtattg aactgccgaa acacgaaaaa ctgcttgccg
     4681 ccgtcgaaaa agtcatttca gacggcctct tggacaacgc cttcaaagcc ctgcttttgg
     4741 gcgtgccgtc tgaagccgag ctgtgggacg gcgcggaaaa catcgacccg ctgcgctacc
     4801 atcaggcgcg cgaagccttg ttggatacgc ttgccgtccg cttcctgccg aaatggcacg
     4861 aattgaaccg tcaggcggcg aagcaggaaa accaaagcta cgaatacagc cccgaagccg
     4921 ccggttggcg cacgctgcgc aatgtctgcc gcgccttcgt cctgcgcgcc gatcccgcgc
     4981 acatcgaaac cgttgccgag aaatacgccg aaatggcgca aaacatgacc cacgaatggg
     5041 gcatcctgtc cgccgtcaac ggcaacgaaa gcgatacgcg caaccgcctg ctggcgcagt
     5101 ttgccgacaa gttttcagac gacgcgctgg tgatggacaa atatttcgcc ctcgtcggct
     5161 caagccgccg cagcgacacc ctgcaacagg ttcaaaccgc cttgcagcat ccgaagttca
     5221 gccttgaaaa tcccaacaaa gcccgctcgc tcatcggcag cttcagccgc aacgtcccgc
     5281 atttccacgc agaagacggc agcggctacc gcttcatcgc cgacaaagtc atcgaaatcg
     5341 accgctttaa cccgcaggtc gccgcccgcc tggtgcaggc gttcaacctc tgcaacaagc
     5401 tcgagccgca ccgcaaaaac ttggtgaaac aagcattgca gcgcattcgg gcgcaggaag
     5461 gattgtcgaa agacgtgggc gaaatcgtcg gcaaaatttt ggattgaggc cgtcaaacag
     5521 aaaaacaatg ccgtctgata tagttacaca cacgttttat catcacttcc ccatcgtttt
     5581 attacgcaat ggcaaaacgg ccgcaaagtc atat
//
LOCUS       DAWFJW010000115         5586 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFJW010000115 DAWFJW010000000
VERSION     DAWFJW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.115"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(407..3154)
                     /gene="tbpA"
                     /locus_tag="WGR19_001769"
     CDS             complement(407..3154)
                     /gene="tbpA"
                     /locus_tag="WGR19_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0799250.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNQYAHFVVEEECKNRNYEACKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANSKQAGSLPGNGKYAGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYFQNAKRAYSLKTPPPNGDKKISPNGSTSTPYWVSIGGGNVVTGQICLFGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINILG
                     KIDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(3241..5337)
                     /locus_tag="WGR19_001770"
     CDS             complement(3241..5337)
                     /locus_tag="WGR19_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0799251.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRFKRRNWHPRVNPKEDKIELSENDWEQTDNGNIKTPS
                     KQKNIINALLGSEGALLQDSSQEGQSISKVTDHHNFQYVWSGFFYKHIQTKFETTGGK
                     MTARSGPDGYIFYKGKDPSRKLPVSGEVMYKGTWDFLTDVKANQKFTDLGNASTKPGD
                     RYSAFSGELDYIVKQENDKKDGHVGLGLTTEITVDFEKKTLSGKLIKNNSVITTNNDK
                     HTTQYYSLEATLKGNRFNGKATATDKPKENEIKQHPFVSDSSSLSGGFFGPQGEELGF
                     RFLSDDKKVAVVGSAKTKDKAESGNGNGTSGGASVSASNGAAGTSSENSKLTTVLDAV
                     ELKSGDKEVKKLDNFSNAAQLVVDGIMIPLLPEASESGSNQANQGTNGGTAFTRKFNH
                     TPKSDEKDTQAGTAENGNPAASNTAGDANGKTKTYEVEVCCSNLNYLKYGMLTRKNSK
                     SAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPNDQNVVYRGSWYGHIANGTSWS
                     GNASDKEGGNRADFTVNFGTKKINGTLTADNRQAATFTIVGDIEGNGFSGTAKTADSG
                     FDLDQSNNTRTPKAYITNAKVQGGFYGPKAEELGGWFAYSDDKQTKNATDASGNGNSA
                     SSATVVFGAKRQKPVQ"
BASE COUNT         1189 a         1429 c         1364 g         1604 t
ORIGIN      
        1 ctagattccc gcctgcgcgg gaatgacgaa tccatccgca cggaaacctg caccacgtca
       61 ttcccacgaa cctacattcc gtcattccca cgaaagtggg aatccagttt tttgagtttc
      121 agtcatttcc gataaattgt cttagcattg aatgtctaga ttcccgcctg cgcgggaatg
      181 acgggatttg aggtttctgt ttttgatttt ctgttttcgc gggaatgacg gtttagaagt
      241 tacccgaaac ctcaaaaaaa ccgaaaccga acggtctgga ttcccgcctg cgcgggaatg
      301 acgggtttcg agattacggt attgtcggaa aggatgggaa acggtgggaa ttgtgtaaaa
      361 aatgccgtct gaagcctttc agacggcatt tgcggcgttt ggacgtttaa aacttcattt
      421 ccaagctaaa tgtgtagttt cggccggggg cggcatatcg gttgtaaacg ccgacatttt
      481 tgtgttggtt gactgcgccg ccggcagttt gccgcacatt ttcccaagta acatagcggt
      541 agttgaggag gttgtacacg cccgcacgga gggtgaagtg ttttttaatc gtgtaataac
      601 cggacacatc cacaatatac caagggcggg tacggcgcgc ggtggctttt gtattgcggc
      661 tgttgccgtt gagcaaagcc cggctgccca acaactctgt gatttccttg gctttggaat
      721 aagtcagcat accgttcaca ccccatttgc cttccggttg gtcatagccc aagccgacga
      781 catagcgcga gggttggatg gcatcaaaca gatgtgattg aatatcggtg cggtctgcgc
      841 gttttttgat gtcgcggaca tggacacgat tataggcaaa tgtagaatac caaccttcgg
      901 gcaatttatc ccatacgccg ttccaatcga ttttgcccaa aatattgatg ccggtaatcc
      961 gcgcgctttg ggcattgagg taagccgggt cgcctttggc ttcttctttg ccgtttttaa
     1021 tttgcgcttc ataaccccgg acaatcaaat cgcggtaggc attgttgaac caacttgcct
     1081 ccaagttgcc gaaatcgcct ttaaacacga tgccggcttc tttgttgaac gatttttccg
     1141 gatcgatttt gaccgccttg ctttgaacac ccgcccgcca gccgtacatt tccgcaaacg
     1201 agggcaggcg gaagccggtt gaagtgcggt aagtcaaatc cagccagtcg gcaggtttga
     1261 ggacgatgcc ggcgttccag gacagggtgc ggtgcgtgcc ggtggaaacg ctgccgtcgt
     1321 ccgaatgcgt gctgcggtag tcgtagcgca accccgcgcc gacatccgcc cacctgccca
     1381 aacggacatt gtcccgaact gccgcgtaat agcttttgcc gttgatgctg cgcggcgtgc
     1441 agtccgtata agtattgttg ccaaagaggc agatttgccc cgtaaccaca tttcccccgc
     1501 ctatgctgac ccaataggga gtgcttgtgc tgccgttggg gctgattttt ttatcgccgt
     1561 ttggaggggg cgttttcaac gaataggcgc gtttggcatt ttgaaaataa taatcctgat
     1621 ggcggagatt agagccaaag cggtcaaacc cgagattcac gctcaggttg tggcggattt
     1681 tggcggtatc gaaggatttt ttgaatgccg cctgcaagag cctgtggctt tccccgtaaa
     1741 tcacgcggtc ggatttgtag taggaaaacg gcttgtcggc actcgggcgg caatatttgt
     1801 ccgaaccatc ggcagaacag tgcgtctgct gaaaatgatt gtccaaaccg ataccctgcc
     1861 ggtcgtaaga gaggcgggca taatccgccc aagtgtcttt atcggcattg gtatagacat
     1921 attccaaacc gtagcggctt ttggtgtgcg tctcgtcgta aaacacgccc gtaccgtatt
     1981 ccgcgcccac cagcgcaccg ttttcaccgt tggtaaacag accgccgtat ttgtggttgc
     2041 ccgcgtattt gccgttaccg ggcaaagaac ccgcctgttt tgaatttgca tcaaaaaccg
     2101 ccttggtcag gaatgccgga accgtcatat cgcgcgtgtc gaaagtttgt tgcgtgtgtt
     2161 cgagtatgcc gccgatgtag tgccgcttat tctcaaaacg aaaacccggg cggaacagcc
     2221 acgatcggct ttcgtatgaa agcggatcgg cgaggaagcg gttggggccc gtgtagtctc
     2281 gggtggaaac cgtttgacgt tcgtctttgc cgacaacatc ttttttcgga ttcgctttac
     2341 acgcttcgta attcctgttt ttacattctt cttcgaccac gaaatgagcg tactgattgg
     2401 tatcctcaac cggcaccagc ctgttaaagc tctgaacgcc gcgtccggca tcttcgtggg
     2461 cgcggatttc ccccgcgcgc cgcccggtgt ggatcagcaa agcctccgca ccgccgatgc
     2521 gccccgccag cgcgatggat tgggtaagcc cccgattttt gccggaatag gcggttttac
     2581 tctgaatgcc ccactgcctg ccttccccga taacatcgtc ggcggttttg gtttgaaatg
     2641 cgaccgagcc cgccaatgcg ccgctgcctt gttcgaccga gtttgagcct ttgctgattt
     2701 cgacagcttt gacgttttca tactcgattt cattgattgc gccgctgctg cccgccgtcc
     2761 tcgtcccgcc caatgccgcc tgcgcggtgt aggactgtat ttgcgccaag ccgtccaccg
     2821 tcaaggaaac gcggtttttg tccatgccgc gtatcgagta gcccgaactt gcgccgcgcc
     2881 cctgttcgac cacggcgatg ccggggtcgt aacgcgtcag gtcgcggata tcgagtacct
     2941 gttccttgct gagggtgtcg gcggttttga ccaatttgcc caaaccggtt acttcgttat
     3001 cgcggcgggt tttctgtttt ttggctttta cctgtatggt atccaactgt ttttcctgtg
     3061 cttgtccggc ttgcacattt tctgcataaa cgggcagcgc agtcattaaa gacaggcata
     3121 aaatatttaa tcggaacaaa tgttgctgtt gcatagtgtt tccctaatct tcgctttcag
     3181 acggtatcgg aaggagcggt gccgtctgag gccttattct tgattgttcg gcaaccgtgc
     3241 ttattgcaca ggcttttggc gtttcgcacc gaatacgaca gttgcactgc ttgctgaatt
     3301 tccattgccg gatgcatctg ttgcattttt cgtttgttta tcgtccgaat aggcaaacca
     3361 tccgcccaac tcttcggctt tgggcccgta aaaaccgccc tgcaccttgg cgtttgtgat
     3421 atatgcctta ggcgtgcggg tgttattgct ttgatcgaga tcaaaacctg agtcagcagt
     3481 tttcgccgta ccggaaaaac cgttgccctc aatatcgccc acaatggtaa aggttgccgc
     3541 ctgcctgttg tcagcggtta acgtgccgtt aatttttttc gtaccgaaat tcacagtaaa
     3601 gtccgccctg ttgccgccct ctttatcgga agcattgccg ctccagcttg tgccgttggc
     3661 aatatgcccg taccaagacc cccgataaac gacgttttgg tcgtttggaa tctctttttc
     3721 atcggtgcgc tcgccttgga ggaacatact ttgttcaact tgttccgttt tagcatcagc
     3781 ttgactactg ctttctcctg cctgcatcgc ggacttgctg tttttgcgcg tcaacattcc
     3841 gtatttcaga taattgaggt tggaacagca gacttcgact tcataggttt ttgttttgcc
     3901 attggcatca cctgccgtat ttgaagcggc tggattgcca ttctccgccg tacctgcttg
     3961 ggtgtctttt tcatcgcttt tcggcgtgtg gttaaatttg cgggtaaagg ctgttccgcc
     4021 atttgtacct tgattggcct gattgctccc gctttcggaa gcctcgggca ggagcggaat
     4081 cataatgccg tcgacaacca gttgggcggc attgctgaag ttgtcgagct ttttgacttc
     4141 cttatcgccc gatttcagct cgaccgcatc caaaaccgtg gtcagcttac tgttttcaga
     4201 cgacgtgcct gccgcaccgt ttgatgcgga aacgcttgca ccgcccgaag taccgttacc
     4261 gttaccgctt tcggctttgt ctttggtttt cgcgctgccg acaacggcaa cttttttatc
     4321 gtcgctcaaa aagcggaaac ccaattcctc accctgcggg ccgaaaaagc cgccgcttaa
     4381 agaagacgag tcggaaacaa agggatgttg tttgatttca ttctcttttg gtttgtcggt
     4441 tgccgttgcc ttgccgttga agcggtttcc cttaagcgtc gcctcaaggc tgtaatattg
     4501 cgtggtgtgt ttatcgttat tagtagtgat tacactgttg tttttaatta attttccgct
     4561 gagggttttt ttctcaaaat caaccgttat ttccgttgtt aatcccaaac ctacgtgccc
     4621 gtctttttta tcattctctt gtttgactat ataatccaac tccccggaaa aagcactata
     4681 tcggtcgccg ggtttcgtag aagcattccc taaatctgta aatttctgat ttgctttcac
     4741 atcagttaaa aaatcccaag tacctttgta cataacctcc cctgaaacag gaagttttct
     4801 cgagggatct ttgcctttat aaaaaatata accgtcaggc ccacttcggg ctgtcatctt
     4861 tccaccagtt gtttcaaatt tagtttgaat atgtttataa aaaaaacccg accatacgta
     4921 ttggaaattg tgatggtccg taaccttaga tatactctga ccttcttgac ttgaatcttg
     4981 caataatgct ccctcacttc caagtaaggc attaataata tttttttgtt tggaaggggt
     5041 tttgatatta ccattatccg tttgttccca atcattttca gagagttcta tcttatcttc
     5101 tttaggattc accctcggat gccaattccg ccgcttgaag cgcattgcaa aaccgtatcc
     5161 gccttggtct ttttgggctt gcggcttttc ggaagaaaca tcttgatatt ttggcgcggg
     5221 acgcggggct tcggtatcga cagaatcaag atcgaaactg ccgcctccgc ccaaacaagc
     5281 actcaacaaa aacacaggca gcaccatagc agcctgattt accaatggat tgttcatata
     5341 gacccaattc aattaaagaa tgataaggat tattatttta tttattttta acaaatttgc
     5401 aaatactttt ttattttttt agggaataca ccaaaatccc gtcattcccg cgcaggcggg
     5461 aatccgaacg cgtccgcacg gaaacctata tcccgtcatt cccacgtata tcccgtcatt
     5521 cccgcgcagg cgggaatcca gtgcattgag tttcagctat ttagaataaa ttttgaaact
     5581 ctaatc
//
LOCUS       DAWFJW010000116         5584 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFJW010000116 DAWFJW010000000
VERSION     DAWFJW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.116"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(144..1940)
                     /locus_tag="WGR19_001771"
     CDS             complement(144..1940)
                     /locus_tag="WGR19_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0799252.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVCLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2398..3651
                     /locus_tag="WGR19_001772"
     CDS             2398..3651
                     /locus_tag="WGR19_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0799253.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIVLTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIASATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMKPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAYVQYGNGEEVQMAGGKYIGQLINM
                     YAVTIGDWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGSEKTGNAELFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3806..5317
                     /gene="lysS"
                     /locus_tag="WGR19_001773"
     CDS             3806..5317
                     /gene="lysS"
                     /locus_tag="WGR19_001773"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0799254.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSRIIQSVRNFMVNEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTAKISYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAESKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1228 a         1419 c         1608 g         1329 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atttgcttgc
       61 ggcaagatga aaacggtgcg ggatgtttta ggaaaccaat gccgtctgaa ggctttcaga
      121 cggcattttt tgcgccgtgc tgtttaacgc gccagcggtt cggtgcgttt gatcagccac
      181 gcttttgccg cgccttcggt cagcggctcg aggcggcggc ggacttcggc gtggtagcgg
      241 ttgacccagt cgatttcgcc gtcggtcatg agggcggtgt ccatcaggcg ggtgtcgatg
      301 gggcagaggg tcagggtttc aaaacagagg aagctgccga attcggtttc ttgaggggcg
      361 gcgacggctt ggttggcggc aaggttttca atgcggatgc cccattttcc cggacggtag
      421 agtccgggtt cgatggaggt aaccatgcct tttttcatgg cggtttcggg cgtggcgggg
      481 gcggcgaagg cgatgcgctg cgggccttcg tggacgttga ggaaatagcc tacgccgtgt
      541 ccggtgccgt ggccgtagtc gcattgcgcc tgccacaggg gtttgcggca aatcgcgtct
      601 atcagtggtg aggggatgtt ttcggggaac acggcttcgg caagcgcgat atgggctttg
      661 aggacgaggg tgttgtcgcg tttttgttct gctgtgggcg taccgacggg gacgacgcgg
      721 gtgatgtcgg tcgtgccgcc tttgtattgc gcgccggagt cgatgagcag cagcccgttg
      781 ccgctgatgg tgctgtggct ttcgggtgtt gcgctgtaat gcggcagtgc gccgttggcg
      841 ttgaagcctg cgatggtgtc gaaactcaat gaaatgaagc ctgggcgcac gctgcggtgg
      901 cgataaagca tggtgtccac gtcgatttcg gtcaggctgc cgccgttgcc gatgatgtct
      961 tcaaactcgg cgaagaaacc gcacaacgcc gcgccgtcgt gttccatcgc ttcgcggatg
     1021 cgggcgatgt cggcttcgga tttgcaggat ttgaacagcg tggacgggtt gatgccttcg
     1081 ataaggcaca cgctttcggg caggcgcaca agcgtgctga cggcggtttt gttcggttcg
     1141 ataagcagcg cgccgccgat ttgcgcgagt ttgtcggcaa cttgggcata aggttcgacc
     1201 gtgatgcctg cggtttgcag cgcggcggcg gcttcggcgt tcagacggca ttgttcggta
     1261 aacaggacgg cgttgtcttt gccaatcagc aggaaggaca cgaaaacagg gttgaaaggc
     1321 acgtcgctgc cgcgcaggtt ggtcagccag gcgatgtcgt caagcgagga aaccaagtgg
     1381 taatccgcgc ctttttccgc catcacggcg cgcacgcggg cgagtttttc ggcggcggtt
     1441 tcggagacgt aggcggggtc gtggatgaac acggtttcgg caggaatggc cgggcggctt
     1501 gtccacactt ggtcaagcag atcgtttggg tgttcgatgc ggatgttttt ggcggcgagt
     1561 gattgcgcca aagtgcgttt gccggtgagc gagaccatat cggaagggat gccgacggcg
     1621 gcgttttcgg gcaggtttgc cgcgagccat tcgttgtacg gcggcacttg accgcttttt
     1681 tgcagctcga tgccgctgcc ggaaagctgt ttggcggctt gttcccaata gcggctgtcc
     1741 acccatacac ccgcttcatc ggtggtcagg acaaatgtgc cgaccgagcc ggtaaagccc
     1801 gacaattcgc ggcgcgcctg ccagtgtgcg ggcaggtatt cggacaggtg ggggtcggcg
     1861 gaagggatga cgagtgcgtc caagccttgc gccctcatgg cttcgcgcaa tgcggacagg
     1921 taattcgata cggtattcat aacagttcct ccaatcgggt tttgcggctt cagacggcat
     1981 ggacggaaat ctgaaatgcc gtctgaaaaa cataaccatc ttatcaaatc gccagcctgc
     2041 tgcaaaacgg acgggctgat cggatggtgc gtgtttgcgc gaaaaaagcg atgtaaggtt
     2101 gtttgttgta aaattttagg cataaaatgt cgtttcatgc ctttttttgg aagtaatatg
     2161 gaaaatatag tcaagtttga gcatgaagtt ctaatagtgt gtgttggcag gtatttggat
     2221 tacgttacaa tacgcgcttt catcaaaatt tccctaccga gcggtatcct gtttttgaag
     2281 acggtaggat ttgggtttgt ttgggggcaa tcccgcccaa attggagcaa tagatagggc
     2341 tgtgtccgaa tatgcggctc tgtgtgttga aacacggtta aaaatacgga agactttatg
     2401 tccgaacaac atatttcgac ttggaaaagt aaaatcaacg cattgggacc ggggattatg
     2461 atggcttcgg cggcggtcgg cggttcgcac ctgattgcct ccacacaggc aggtgcgctt
     2521 tacggctggc agctcgcgtt gattatcgtc ctaaccaacc tcttcaaata cccgtttttc
     2581 cgcttcagcg cgcattacac gctggatacg ggtaaaagcc tgattgaagg ttatgcggag
     2641 aaaagccgcg tttatctttg ggtattcctg attttgtgta tcgcctccgc caccatcaac
     2701 gcgggcgcgg tcgccattgt aaccgctgcc atcgtcaaaa tggcgatacc ctcgctgatg
     2761 tttgatgccg gcacggttgc cgccttgatt atggcatcct gcctgattat tttggtgagc
     2821 ggacgttacc gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc
     2881 acgcttgccg ccgccggcat cgctatgtcg cgcggtatgc agatgaagcc ggactttatc
     2941 gaaccgacac cgtggacgct tgccggtttg ggctttctga tcgcgctgat gggttggatg
     3001 cctgcgccga ttgaaatttc cgccattaat tctttgtggg taaccgaaaa acaacgcatc
     3061 aatccttctg aataccgcga cgggattttt gatttcaacg tcggttatat cgccagtgcg
     3121 gttttggctt tggttttcct tgcactgggc gcgtatgtgc aatacggtaa cggtgaagaa
     3181 gtgcagatgg cgggcggcaa atatatcgga caactgatca atatgtatgc ggtaaccatc
     3241 ggcgactggt cgcgtccgct ggtggcgttt atcgcgtttg cctgtatgta cggtacgacg
     3301 attaccgttg ttgacggtta tgcacgcgcc attgccgaac ccgtgcgcct gctgcgcggc
     3361 agtgagaaaa ccggcaacgc cgaactcttt gcctggaata tttgggtggc gggcagcggt
     3421 ttggcggtga ttttctggtt tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt
     3481 gccgcctttg tgtccgcccc tgtgtttgcc tggctgaatt accgtttggt caaaggtgat
     3541 gaaaaacaca aactcacatc aggtatgaat gcccttgcat tggcaggctt gatttatctg
     3601 accggtttta ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg ataacgatgc
     3661 cgtctgaaac cgcaaaccgc tttcagacgg cattgccgcg tttatggaaa aaaatgccga
     3721 catcgcgtaa aatatgcgca aattttgtga ttcggtcagg tcgtctgaaa cagattgcgc
     3781 ctgatttatt ttttcggaaa accctatgag cgaacaaaac catccgcaaa ccgagccgca
     3841 gttggacgaa aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca tccgccaaca
     3901 gcgcaacgcc tatcccaacg acttcaaacg cgacagcttt gccgccgatt tgcacgccca
     3961 atacggcgaa atcggcaaag aagaactcga tccgcaaggc attcccgtca aagtggccgg
     4021 ccgcatgatg ctgaagcgtc aaatgggcaa ggcgagcttt gccaccattc aagacgtgac
     4081 cggtcaaatc cagctttatc tgaacaacaa aggcgtgagc caagaagttt tggacgactt
     4141 caaccattgg gatttgggcg acatcgtcgg cgcggaaggt actttgttta aaaccaatca
     4201 cggtgaattg accgtgcgcg tatccggcat ccgcctgctg tccaaatccc tgcgcccgct
     4261 gcccgacaaa cacaaaggct tgagcgatca ggaaaccaaa taccgccaac gctacgttga
     4321 tttgattgcc aacgaagaat cgcgcaatac ctttatcaag cgcagccgaa tcatccaatc
     4381 cgtgcgcaat ttcatggtta acgagcatta tttggaagtt gaaaccccaa tgatgcaccc
     4441 gattccgggc ggcgcgacag cgaaaccgtt cgttacccac cacaatgcct tggacattcc
     4501 gctctatctg cgcatcgcgc ccgagctgta tctgaaacgc ttggttgtcg gcggtttgga
     4561 acgcgtgttt gaaatcaacc gcagcttccg caacgagggc atgtccgtac gccacaaccc
     4621 tgaattcacc atgatcgaat tctacgaagc cttctccgac tacgaacgca tgatgcaaat
     4681 ggcggaagac atcatccgca acgcttcccg cgcggtaaac ggtacggcaa aaatcagcta
     4741 caacggcaaa gaagtcgatt tggaaagccc gtttgagcgt ctgaccatcc tcgaagccat
     4801 caaaaaatac aatccgcact acaccgacga gcagttgaac gatgcggaat ggctgaaaaa
     4861 agaaatcgtc aaacacggcg aaagcctgcc gccgtccccg ggcatcggca gcctgcaact
     4921 cgccctgttt gaaggttgcg ccgagagcaa attgtggaac ccgaccttca tcgtcgatta
     4981 tccggtcgaa gtttccccgt tggcacgcgc ttccgatacc aaacaaggtc tgaccgagcg
     5041 tttcgaattg ttcgttgtcg ggcgcgaact ggcaaacggc tattccgagt tgaacgaccc
     5101 cgaagaccaa gccgaacgct tcaaagcgca agtggcgcaa aaagacgcgg gcgacgacga
     5161 agccatgcat tacgatgccg actacatccg cgcgatggaa ttcggtttgc cgccgaccgg
     5221 cggttgcggc atcggtatcg accgcttggt aatgctgctg accgattcgc aaaccatccg
     5281 cgatgtgatt ctgttcccgc agatgcgtcc agagtaatca taaaaacagt tgaaatagaa
     5341 atgccgtctg aacccgattg gattcagacg gcattttgta tggcaggatt gatagtgaat
     5401 taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagagac ctttgcaaaa
     5461 ttcctttccc tcccgacagc cgaaacccaa acacaggttt tcagctgttt tcgccccaaa
     5521 taccgcctaa ttttacccaa ataccccctg aatcctcccc ggatacccga taatcaggca
     5581 tccg
//
LOCUS       DAWFJW010000117         5572 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFJW010000117 DAWFJW010000000
VERSION     DAWFJW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.117"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(254..676)
                     /gene="queD"
                     /locus_tag="WGR19_001774"
     CDS             complement(254..676)
                     /gene="queD"
                     /locus_tag="WGR19_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0799255.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGG"
     gene            complement(683..1201)
                     /locus_tag="WGR19_001775"
     CDS             complement(683..1201)
                     /locus_tag="WGR19_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0799256.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(1229..1891)
                     /gene="queC"
                     /locus_tag="WGR19_001776"
     CDS             complement(1229..1891)
                     /gene="queC"
                     /locus_tag="WGR19_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0799257.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYRRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDEAAAIETAGNGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIVGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGGCRECPSCILRERGLAEYLESKK
                     AV"
     gene            1996..2421
                     /locus_tag="WGR19_001777"
     CDS             1996..2421
                     /locus_tag="WGR19_001777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799258.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2436..3662
                     /locus_tag="WGR19_001778"
     CDS             2436..3662
                     /locus_tag="WGR19_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0799259.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(3683..3964)
                     /locus_tag="WGR19_001779"
     CDS             complement(3683..3964)
                     /locus_tag="WGR19_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0799260.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(4043..4177)
                     /locus_tag="WGR19_001780"
                     /pseudo
     CDS             complement(4043..4177)
                     /locus_tag="WGR19_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(4176..4634)
                     /locus_tag="WGR19_001781"
     CDS             complement(4176..4634)
                     /locus_tag="WGR19_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799261.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(5198..5401)
                     /locus_tag="WGR19_001782"
     CDS             complement(5198..5401)
                     /locus_tag="WGR19_001782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799262.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT         1340 a         1290 c         1367 g         1575 t
ORIGIN      
        1 cacttgaaat ccgaatccaa ggtcgtctga aaaaacgggg aatccgtaca ggagaaaaaa
       61 ccgttttacg gtaaattcaa ataaggacgc tctaaaccgt cctttccgcg caggcgggaa
      121 tccagagagt tggaattgct gcaattttta aatattcctg aaaaaccaag gtctggattc
      181 ccgcctgcgc gggaatgacg gcttagggca agttttattc aaattcacta tatcgatacg
      241 ttcaaactat cccctacccc ccttcatact ccgcacacga tgtcggcgtt tcccacaatt
      301 tgacgcggca cacgttcagc cccgcgtttt tcagacggtc gtacatttcg accgccatat
      361 tttcggcagt ggtgcggcag ggcaggcgca gggttttcat gttccagcct tccaagagtg
      421 cggcgatttg agattcgcgc ccgttgccgc cgtggtagat gaaggcgtgg tcgaaggggt
      481 cggtaatgtg ttgtttgaca atggctttca agtcggtaaa gtccatcacc ataccgtctt
      541 tcggcccgcc tctgacgggg tcgtctgaaa cggtgatttc gagtttgtag gtatgcccgt
      601 gcaggttttg gcatttgccg tcatgcccgt cgagcatatg cgaggagtcg aaggtgaaga
      661 ttttggtgat tttcatgggt tctcaaatgg gcaaatagcc ttgaaagccg atttcggcgg
      721 gtttgccgtg cggattcggg gtcaggcgga tatggaaatt gccgatgcgg tcgagcgcaa
      781 gcaggttgtc cacgtctttt aagttgtctt tatgttgctg aacatagctg tcggtcaaca
      841 gggtttgcag ggctttttgt ttggcttcgg caggcgtggc ggcggcgaac agcccaaaag
      901 cgtgcgcttc ggcgagctgc cctgcgcgat aaccgccgac attggcaaaa tacaggcgcg
      961 gggcgtgttc agacggcatt ttcgttttgg gcgtttcgga tacggcgatg tcgtaaccgt
     1021 ccgcccattc gacaatctgc cagccgtcga tgtggatttt gtccgcatcg ccgaaccacg
     1081 cggctttgag tgcggggacg gcttcacggt agtcgtcgca cacggcaaat tggatgtcgt
     1141 gcacttcgat attcgacctg ccggcattgc cgccgaggta aaacatatgg agcttgggca
     1201 tatcgtattc cttatggttt gcgcgtgttc agacggcctt tttactttcc agatattccg
     1261 ccagcccgcg ttcgcgcaag atacagctcg ggcattcgcg gcagccgccg ccgacgatgc
     1321 cgttatagca ggtgtgggtt tgctcgcgga tatagtccag cgcgcccatt tcgtccgcca
     1381 acgcccaagt ttgcgccttg gtcagataca tcagcggcgt gtggatttga aaatcatagt
     1441 ccatcgccaa attaagggta acgttcatcg atttgacaaa cacgtcgcgg cagtcgggat
     1501 agccggagaa gtcggtttcg cacacgccca cgatgatgtg ccgtatcccc tgccctttgg
     1561 cgtaaatcgc ggcgtaaagc aggaaaagcg cgttgcgccc gtccacaaag gtattgggaa
     1621 caccgttgcc ggcggtttcg atggcggcgg cttcgtccat cagggcattg tgcgtaatct
     1681 gccgcatcag gctcaaatcg agtacggttt gactgacacc caaatcctgc gcgatccact
     1741 ctgcgcgttc cagctcaacg gcatggcgtt gcccgtatcg gaaggtgatg gcttggacgt
     1801 tttcgcgtct gtaagtttgg attgcctgaa tcaggcaggt agtggaatct tggccgcccg
     1861 aaaagatgac caaggctttt tggtttgaca tatcgaatcc tagttttggt aacgcgggag
     1921 gtttgcgaac cgcgaagttg gatagtaaag acgcggatta tacaggcaaa cgcgttcaga
     1981 cggcatctga acgccatgcc gtctgaacac gcggcttatg ccgacttgac ggcgagttcc
     2041 gccaagcgtt tgctttgtgc gaaggcgata ttgttttctt cggcggtcag gacgggagcc
     2101 gctgtcggga atatcttttt tgacggcttc gcagacggcg gagactttgg ggacggtgcg
     2161 caatacggct tcgcaacctt caacgctgtc gatgccgcga gcgattcgga gtgcgggatt
     2221 gcgggtgctg ccgttttgga aatagtagag gacgaggatt ttgaggggga tttgggttca
     2281 tgggcggatt ccgttaaaat ggttcggtct tcagacggca taacagttgt tccgatgccg
     2341 tctgaaacag ccgcagttca ggcatttgtt cctgacggca aaattggcta taacatcggg
     2401 catcaatgcc caacttactg accgggcaag aaattatgac ctttttacaa cgtttgcaag
     2461 gtttggcaga caataaaatc tgtgcgtttg catggttcgt cgtccgccgc tttgatgaag
     2521 aacgcgtacc gcaggcggcg gcaagcatga cgtttacgac gctgctggca ctcgtccccg
     2581 tgctgaccgt gatggtggcg gtcgcttcga ttttccccgt gttcgaccgc tggtcggatt
     2641 cgttcgtctc cttcgtcaac caaaccattg tgccgcaggg cgcggacatg gtgttcgact
     2701 atatcaatgc gttccgcgag caggcgaacc ggctgacggc aatcggcagc gtgatgctgg
     2761 tcgttacctc gctgatgctg attcggacga tagacaatac gttcaaccgc atctggcggg
     2821 tcaattccca gcgtccgtgg atgatgcagt ttctcgtcta ttgggcttta ctgacgttcg
     2881 ggccgctgtc tttgggcgtg ggcatttcct ttatggtcgg ctcggtacag gatgccgcgc
     2941 ttgcctcagg tgcgccgcag tggtcgggcg cgttgcgaac ggcggcgacg ctgaccttca
     3001 tgacgctttt gctgtggggg ctgtaccgct tcgtgccaaa ccgcttcgtt cccgcgcggc
     3061 aggcgtttgt cggggctttg gcaacagcgt tttgtctgga aaccgcacgt tccctcttca
     3121 cttggtatat gggcaatttc gacggctacc gctcgattta cggcgcgttt gccgccgtgc
     3181 cgttttttct gttgtggctg aacctgttgt ggacgctggt cttgggcggc gcggtgctga
     3241 cttcttcact ctcctactgg cagggagaag cgttccgcag gggcttcgac tcgcgcggac
     3301 ggtttgacga cgtgttgaaa atcctgctgc ttctggatgc ggcgcaaaaa gaaggcaaag
     3361 ccttgcctgt tcaggagttc agacggcata tcaatatggg ctacgacgag ttgggcgagc
     3421 ttttggaaaa gctggcgcgg cacggctaca tctattccgg cagacagggt tgggtgttga
     3481 aaacgggggc ggattcgatt gagttgaacg aactcttcaa gctcttcgtt taccgtccgt
     3541 tgcctgtgga aagggatcat gtgaaccaag ctgtcgatgc agtaatgatg ccgtgtttgc
     3601 agactttgaa catgacgctg gcagagtttg acgctcaggc gaaaaaacag cagcaatctt
     3661 gagtattttt gaaactgtat ttttatccta aagacatttt cctatctgct gattccaccg
     3721 tttcctttct gtgtgccgcc ataattttta tgatagacaa accattcaag tttgcattga
     3781 ctgctttttc attggcaata tccaaatggc tggtcgctgc atctaaaaat aggattttcg
     3841 gttcgcaata taaggcccgc gccaatacga tgcgttgttt ttgtccgcct gacagtgcgc
     3901 tgcccatatc gccgatcaag gtttcatagc ccataggcat ggcggagatt tcttcgtgaa
     3961 tcatagcaag gcaggcgcaa tgttcgattt ttcctctatc cggttccgta tcgaaaaaac
     4021 aaggggctgt actagattag ccctaaatcc cacaccaatc ccgcaggatt ttaagctgtt
     4081 gagacggtgt gccgaagtta aatcgaaatt cgcattcttt caagaacagc gggaaagatt
     4141 tacgatcgat tccgttgtat tttcgcaaga cgcgtttagt ctagagtctg tatattacat
     4201 ttatttttag ggtctgctag ccaatttctt gttcccttca ttattttatc ttctgaaaga
     4261 aaattatttt tttccatgct attaatatta atgatatgat ttttatttaa aataattttt
     4321 ccatataaaa tttccggttt gtaatagcaa gagtctgaag caaatccttg cccgtcaata
     4381 taagaaaaat aaatgttatc atcctcctta taatattcta aaaccaatcc ggatattatt
     4441 ttatcaaaat tcccttcatt tccatctttc atgactaaaa tataattagc aggccaacct
     4501 ttatcgtaaa catattcaaa ctgatagttc ccgaaactct cgatatccga actaaaaaag
     4561 aagaaaagca aattaatagc caatacaaaa acaaataaga acaaaaatag caaaattttc
     4621 aacttagtta acaatattta cctctccttt aaattcaatc ctgaaaggta ccccttaccc
     4681 tgggctacca attatagttt ccatatttct aaatattgtt tttacattac ttttttctcc
     4741 ccccaaagga atgcatttta aaatcatgct tttcaggtgc taatcgatac ttaccattaa
     4801 catctttaat cacagatata tttccatgta tagcccaacg tgaaaaatct gagtattata
     4861 tacagttata ccttactgaa acaaagctag ctgtacatat ctgtaccata tttttactac
     4921 aaacaaaaaa tatttataat tattaaggat aagataaaaa cccccaacgc tagattaatt
     4981 cttaataatt tcaaataaaa aatatttgag attttttctt ttccaaataa tatattgaaa
     5041 tatttaaagt taaggtagct tgatttaagt agaaaatcaa aaaaaccatt aattaatttt
     5101 tcttctttaa atattttata tgatattata aaattaaata acattgcgaa aacatatgaa
     5161 atcatcaaaa taccaaatat atagtgggaa tgcattatta tttagctcct tcttttggct
     5221 ctgacaaatt gacagtccat tgatatccaa tgcttgcatt tgcaattgct gacgcacctg
     5281 cccctgtttt ccctgttagt atcaatggtt cttttcttgc aagatgtgca cctgcacaac
     5341 cacctaatac taaaccacca cctattgtaa atccttcctg ttgtatctct aatgataaca
     5401 ttttcttctt gtgccgccct taatacagat tgtggaattg gattgacctg agcaggaact
     5461 tctaaaataa gttttccttc aagctgtctt cctcttagcg aagcaggtgt acttggaaca
     5521 ttggaaatga caaagtgagt gcgcaaagcc acctgaaggc gatgtgtttg aa
//
LOCUS       DAWFJW010000118         5567 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFJW010000118 DAWFJW010000000
VERSION     DAWFJW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.118"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(104..622)
                     /locus_tag="WGR19_001783"
     CDS             complement(104..622)
                     /locus_tag="WGR19_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0799263.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDTILNQKECFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(637..864)
                     /locus_tag="WGR19_001784"
     CDS             complement(637..864)
                     /locus_tag="WGR19_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799264.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            complement(928..1095)
                     /locus_tag="WGR19_001785"
     CDS             complement(928..1095)
                     /locus_tag="WGR19_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799265.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(1088..1261)
                     /locus_tag="WGR19_001786"
     CDS             complement(1088..1261)
                     /locus_tag="WGR19_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799266.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(1370..1636)
                     /locus_tag="WGR19_001787"
     CDS             complement(1370..1636)
                     /locus_tag="WGR19_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799267.1"
                     /translation="MQVLKKVDWKMFVARSFWQCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            complement(1726..2640)
                     /locus_tag="WGR19_001788"
     CDS             complement(1726..2640)
                     /locus_tag="WGR19_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ0799268.1"
                     /translation="MKQINHTLQQKLAEFKTKSGMNQTQLARGIGTSPASISMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKARREELVLGFVSTKTTRRIVEAMRNAHEGGEIS
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGHLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKYG
                     ELVQLHSRVAGALNLGDTMPDAELEQIARATLPEADDETIAELVKHSNGNTRRMSKLM
                     RGSVRVANKNGIRMQAGIVKKYSTLIIR"
     gene            complement(2676..4667)
                     /locus_tag="WGR19_001789"
     CDS             complement(2676..4667)
                     /locus_tag="WGR19_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799269.1"
                     /translation="MGITIGELLKLNIQGLPTTARGLRERAKTQNWPYIEEVGKARGG
                     RLKKYLIASLPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEK
                     QRSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSR
                     SAKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHSIPSE
                     PKLAHSYRRFVQWAEAENMPVNDVPSLSMVRRVWGKLPLIMQERGRKTGAAYKSLLPY
                     VKRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAE
                     SCVAVSDALRIGVKHFGLPIIYYSDNGGGQIGKTIDHEITGITSRLGIRHETGIAGNP
                     QGRGIIERWWKDNLIEMARQYETFTGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQ
                     QKYLKKLPSWSRFIADVAKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAP
                     DMLSAEELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVN
                     VFDMDGKFITKAQADGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALE
                     QAAAWDELGHLGGNDIEAEYAVLPKTGTDDFVLFEADRS"
     gene            complement(4678..4866)
                     /locus_tag="WGR19_001790"
     CDS             complement(4678..4866)
                     /locus_tag="WGR19_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0799270.1"
                     /translation="MGIKTVNEVREWLKDNNLTVAQWARENGHNSVDVTRVLTGKSKC
                     RYGQGRAIAIKLGLRIDE"
     gene            4986..5348
                     /locus_tag="WGR19_001791"
     CDS             4986..5348
                     /locus_tag="WGR19_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0799271.1"
                     /translation="MDNSFELSSKLLGERLKKIRTDNGYSREQLSELFGISRASIQNY
                     ENGERSPNADYLVQFYKHFGINLHWLLTGNKAAKFQDFIDSVSSPREEVLLHLARQMD
                     SRTLNHLIDFLMDVYAKK"
BASE COUNT         1260 a         1597 c         1114 g         1596 t
ORIGIN      
        1 ctttaaatca tgggttaaag cctatttgaa ccggctaggc aagctcctgc tccgtaggct
       61 caatcacgtt tcaacacact gaattgaaat caggctgttg attttataga atttgttcgc
      121 tcggctcaat cacaaaatcc tccacgccgc tcacaatctt aatccccgga acttggccgt
      181 ctgaaaaaca ctctttttga ttcaggatgg tgtctttgtc gatttccttt ttagtgcgga
      241 caaactcggc aaaggcggat ttttccgaga gccacgccag gacggcggcc acgcccgtta
      301 ccttgacaga tggcggacgg atgcgccatt taatcaggcc ggtggtaaag tccacggttt
      361 tggttttacc attttccgtc agctcgtcct tatgcgcttc gcagtaggcg gccacacgtt
      421 cggtcaggct catgatttcg gcacacatcg gcgcggcttt ggcggcatat tcttcttcga
      481 tagccgcttt tttgtctccg gcttcggttt ccaggcgttt gacttcgcgc tgcaagtcgc
      541 cgatttggcg gataaatgca gtaacttccg ttttgtcttg tgccgcttcg atggcgggct
      601 gtttgattcg ggttttagcc attttctctt ccttccttaa ttgaagattt tttgatatgt
      661 ctggattgca gccaattgat ttgatgcgtc gattacgaat ttgcgacgat ctttttgggc
      721 atcacgtaac atacttgcaa taaattccgt ggttagccca gcaatcaaat caacttcgtc
      781 aatttgctta cccattggga tttcggggag ttctatcttt aacttaccgt tttcattact
      841 aattttaaaa acatacactt tcatttcact ttcctttctt gtttaaaatt gcttcaactt
      901 tcgcgcggtt tctcaaaaac ttaaacatca cacttcctcc cataaagtta tcgcctgagc
      961 caaagtttcc gcctcggcag tcttccacat cccgtccggc gcacgcgctg caatcacaaa
     1021 gccttcgccg tccttcttca tgaccatcag ctcgccacgg tcttcgagcc attcgattaa
     1081 atctttttca ttcatctttg tttccttccg ttttcagacg acctttgccg tccttatctt
     1141 caaactgcgc ctgatattcc tcgatttcgc gctcccggct tttttgcgcc attcgcgccg
     1201 tcgcacgcct gcggtgttgt ccccaagcct gccaatccgt attacgtcgt ccgaaactca
     1261 tttcacacat cctttcacaa tcgccttatc gccatatttc gcgcggattt cctttaccgc
     1321 ccgtgccaaa gcctcttttt tcgccgcagg gctcaataaa attggcttat cactcataaa
     1381 caatcccttt cattttttcc tcgacgctca ttgactcgta ttgcccttcc cataccttga
     1441 cctccaaatc cgcctgtctt tccatcgcct caaccttcgt cggctctttc gcaaccggtt
     1501 cgggttcttt cgcgcaggaa tgcaacgcca ttcccgccac aaaacaccac acgcccgccg
     1561 tcaaaccaac cggcacacac tgccaaaaag agcgtgccac aaacatcttc caatcaactt
     1621 tcttcaaaac ttgcattttg agttttcctt tagaaaacaa taacttatta aaatcatagg
     1681 gtaaaaaata tatagccctg tcagagactt accgtttcag acggcctatc ggataatcaa
     1741 tgtcgaatat tttttcacga ttcccgcttg cattcgaata ccgtttttat tcgctacccg
     1801 tactgatccg cgcatcaact tgctcatacg ccgtgtatta ccattgctat gcttaaccag
     1861 ttccgcaatc gtttcatcat cggcttcggg tagggttgcc cgcgcaatct gttccaactc
     1921 tgcgtcgggc atcgtatcac ctagattcag cgcacccgcc acacgactgt gaagctgtac
     1981 caactcgcca tacttaccgc gcaaattcgc caccagtcgg ggcataccgc tcaacaccaa
     2041 accgcagcca gtctcatcgt gcagtcggcg gataatctca agggcgcgta atggcaggtt
     2101 ttccgcttca tcgaccacaa tcagatggcc tgaatcacgc aatctgtccg ataccgattc
     2161 aaacaaatca ttcaagctgc ccatcgccga taccttcgcc gccgtcgcca acttgcgcat
     2221 caagaccaaa gccgtaaagc tcggattagc ctcaatcaag atggctgcag ggtttttctc
     2281 gcagtagttt ttgaccgcct gcgtcttgcc caaacccgcc tgaccgtaga taacggaaat
     2341 ctcgccgcct tcgtgcgcat tgcgcatcgc ttccacgatt cggcgtgtcg tcttagtcga
     2401 tacaaatccc aacaccagct cttcacgtcg cgccttactg tcctgcatct ccaaaaacgc
     2461 ctcgattttc ggctcgatgg tttcataatt gccgcctttt tccgcataag tgccgttcag
     2521 atacatactg atggatgccg gcgaagtacc gataccgcgt gccagttggg tttggttcat
     2581 gcctgatttg gttttaaatt cagccagttt ttgttgcaat gtatgattga tttgtttcat
     2641 ttttttagtc cttttaaaag aggtttaaaa ccgttttaac ttctatccgc ctcaaacaac
     2701 acaaaatcgt ctgtgcccgt tttcggcaat accgcatact ccgcctcgat gtcgtttccg
     2761 cccaaatgtc ccagctcgtc ccaagctgcc gcctgttcca gtgccggatt gacttccgcg
     2821 tttgcgagct tgattgcatt ttccgcccgc ttgattttgc cttttcggcg tttttccgcc
     2881 agttggtcga tacgagccgt cgggaaagcc tcgcgggtat tgccgtcggc ctgcgcctta
     2941 gtgataaact tgccgtccat atcaaacaca ttgaccgccg acgcatcgct caaatcgtag
     3001 ctgacccgta cctcgtcttt gtgatactcc gccagctcag ttgagaaata agagttgttg
     3061 aacaaatcca gccaaccgcg ctgtaccttt cgcacctctt gcggcataaa catcgtcgcc
     3121 agctcttctg ccgacaacat atccggcgcg ataccgtcct gttccagcct catttcccga
     3181 taagccttcg gcgaataatg cccgccgtca ggatgtcggg gcagctcgcc gtgcgggcgg
     3241 ttgttgtatt cgtcgataca cttggccaca tccgcgataa agcgcgacca gctcggcagt
     3301 tttttcaaat atttctgttg ttcctccgtc aaatccttgc ctttttccaa agcattaaaa
     3361 gcactttcca tcttgcggta catcaggttc ttcgtgctgc tgtccatccc cgcgcccgta
     3421 aacgtctcat actgtcgcgc catctcaatc agattgtctt tccaccatcg ctcaatgatg
     3481 ccgcgccctt gcgggttgcc cgcgataccc gtttcatgcc ggatacccag tcgggacgtg
     3541 atacccgtga tttcatggtc tatcgtcttg ccgatttggc cgccgccgtt atccgagtag
     3601 tagataatcg gcaaaccaaa gtgcttgacc ccgatacgca gagcatccga taccgccaca
     3661 caactttcag ccaacgacac cgaaaaaccg accacaaacc gcgtacaacc atcaataatc
     3721 accgtcactt ccggcttaaa cggtctgccg tgtacagggt gcgccacctt cgccttaaag
     3781 ctgtggccgt cgccgatcca aacatcgttc ggcttcaaag ccccccaatc acgtttcaca
     3841 taaggcagca gcgatttata agccgccccc gttttcctgc cacgctcctg cataatcagc
     3901 ggcagcttgc cccaaacgcg ccgcaccata ctcaagctag gcacatcatt gaccggcata
     3961 ttttccgctt ccgcccactg cacaaaccgg cggtagctgt gcgccagttt cggctcggat
     4021 gggatactgt gaaactgcat aaacatcggc aaccaaccgt agctctcaat cggcttaacc
     4081 gccttcgtcg tcttcggagc caaagcaacc aaccgctctg tcgcgttttc cgctttcaaa
     4141 taagcagaaa tccagccgtc taaagtacgt tcgccaacct tcgccgaccg gctgcggtca
     4201 ttggcctttt ccaagttccc aagcgtgacc gcgtccaatt taccttctgc cagcaagccc
     4261 aaaaactgag ccaccgcagc cttggcagag caatcgtatt cgtatttaat ccccaatacc
     4321 gccgccacca ccgcacatcg cgcatccgcc accgaccgtt gtttctcgtt caacagcttg
     4381 gccgcttcag ccagtgcctg agccgacatc gccgtccccg gtctgacttg gggcagggtt
     4441 ttcggcatct tctccgccag ctcgtccgac tgccgtttca tgatggctgc tcggatttcg
     4501 gcagggaggg aagcgattaa gtattttttc agacggcctc cacgcgcttt gccaacctct
     4561 tcgatgtacg gccagttctg ggtttttgct ctctccctta atcctcttgc tgttgtagga
     4621 agcccttgaa tattaagttt aagcagctca ccaatcgtta tccccatctt cgtttcctca
     4681 ctcgtcaatt cttaacccca gcttaatcgc gatagcgcgc ccctgaccgt atcgacactt
     4741 tgatttcccc gtcaacacac gagtaacatc tacggagttg tgtccgtttt ctctcgccca
     4801 ttgcgcaact gttaaattat tgtcttttaa ccattctcta acttcattta ctgttttaat
     4861 tcccattttt ttacctttct tggtttgttc aaatcctgta taattactac aaaactagct
     4921 gttttgctac atttgtagca atattattag ctacaaatgt agctgtcaag aaaaaaggaa
     4981 cttttatgga taattctttt gaattaagct ctaagttatt gggtgagcga ctaaaaaaaa
     5041 tcagaacaga taatggatac tcaagagagc agttgagtga gttatttggc atcagtaggg
     5101 caagtattca aaattatgag aatggggaaa gaagtccgaa tgccgattac ttagttcagt
     5161 tttataagca ctttggaatt aatttgcatt ggctgctaac tggaaataag gctgccaaat
     5221 tccaagattt cattgattct gtttcttctc cgagagaaga agtactacta cacttggctc
     5281 gccaaatgga tagtcgcacc ttgaatcact tgattgactt tctaatggat gtttatgcta
     5341 aaaaataaac agcactaagg aaaactcagc ttttaagttc cttccttagt gaacagtcgc
     5401 ctaaatagag aaattttttt taaattttga ttttgaaatg aaaaatacaa aggaaggctt
     5461 ctaaacctct tcctttgtat tttcctttgt cttgcaagaa tctacaggct gagatttaaa
     5521 ctggctgctg accggcaaag gcttaccgta tcttgataaa gccaggc
//
LOCUS       DAWFJW010000119         5525 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFJW010000119 DAWFJW010000000
VERSION     DAWFJW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.119"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>5525
                     /locus_tag="WGR19_001792"
     CDS             <1..>5525
                     /locus_tag="WGR19_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ0799272.1"
                     /translation="NVAVNAEGKLVNTGMIAATGENHAVSLHARNVHNSGTVASQDDA
                     NIHSQTLDNSGTVLSSGQLTVRNLGRLKNHNNGTIQAARLDMSTGGLDNTGNITQTGS
                     QALDLVSAGKFDNSGKIGVSDVPQTGLNPNPSVIPQIPSTATGSGSSTVSASKPGSNN
                     PVSPTAPAKNHAGGRIQTTGAFDNAGSINAGGQIDIAAQNGLENSGSLNAAKLRVSGD
                     SFNNTVKGKLQAHDLAVNTQTAKNSGHLLTQTGKIDNRELHNAGEIAANNLTLIHSGR
                     LSNDKKGNIRAAHLQLDTAGLHNAGNILADSGTVTAKNNLRNTGKVSVARLNTEGQTL
                     DNTHGHIEAETVNIQSQQLTNQSGHITATEQLTINSRNVDNQNGKLLSANQAQLAVSD
                     GLNNQHGEIATNRQLSIHDKNQNTLALNNADGTIQSAGNVSLQAKSLANNGTLTAGNK
                     LDIALADDFVVERDLTAGKQLNLSIKGRLKNTHTLQAGHTLKLNAGNIDNQVTGKIIG
                     GEQTDITSEQHVGNRGLINSDGLTHIGAGQTLTNTGTGKIYGNHIALDAQILLNREET
                     TEGSTKAGAIAARKRLDIGAKEIHNQEGALLSSEGIFAVGNRLDEQHHAAGMADTFVN
                     GSAGLEVQGDALMSVRNMQNINNHFKTETYLAKAEKQVRDYTVLGQNTYYQAGKDGLF
                     DNSQGQKDQTTATFHLKNGSRIEANQWHVRDYHIETYKERIIENRPAHITVGGDLTAS
                     GQNWLNKDSRIVVGGRIITDDLNQKEITNQSTTGKGRTDAVGTQWDSVTKKGWYSGRK
                     RQRRTERNHTPYHDTQLFTHDFDTPVSVIQQNAASPSFQPAASAIKLIDGVSTAAVNG
                     QRIHTGNVVSLNNATVTLPNSSLYTTHPDNKGWLVETDPQFADYRRWLGSDYMLQQLQ
                     LDTNHLHKRLGDGYYEQKLVNEQIHQLTGYRRLDGYRSDEEQFKALMDNGLTAAKTFA
                     LTPGIALSAEQVARLTSDIVWMENQTVTLSDGSTQTVLVPKVYALARKGDLNTSGGLI
                     SAEQVLLKLQNGNLTNSGTIAGRQAVLIQARNINSNGNIQADQIGLKAEKSINIDGGQ
                     VQAGRLLTAQAQNINLNGTTQTSGNERNGNTAIDRMAGINVVGSHTEQVDNRTSDGIL
                     SLHAGNDINLNAATVSNQVKDGTTQITAGNNLNLGTIRTEHREAYGTLDDENHRHVRQ
                     STEVGSSIRTQNGALLRAGNDLKIRQGELEAEEGKTVLAAGRDVTISEGRQITELDTS
                     VSGKSKGILSSTKTHDRYRFSHDEAVGSNIGGGKMIVAAGQDINVRGSNLISDKGIVL
                     KAGHDIDISTAHNRYTGNEYHESKKSGVMGTGGLGFTIGNRKTTDDTDRTNIVHTGSI
                     IGSLNGDTVTVAGNRYRQTGSTVSSPEGRNTVTAKSIDVEFANNRYATDYAHTQEQKG
                     LTVALNVPVVQAAQNFIQAAQNVGKSKNKRVNAMAAANAAWQSYQAAQQMQQFAPSSS
                     AGQGQNNNQSPSISVSITYGEQKSRNEQKRHYTEAAASQIIGKGQTTLAATGSGEQSN
                     INITGSDVIGHAGTALIADNHIRLQSAKQDGSEQSKNKSSGWNAGVAVKIGNGIRFGI
                     TAGGNIGKGKEQGGNTTHRHTHVGSTTGKTTIRSGGDTTLKGAQLIGKGIQADTRNLH
                     IESVQDTETYQSKQQNGNVQVTVGYGFSASGSYRQSKVKADHASVTEQSGIYAGEDGY
                     QIKVRDNTDLKGGIITSTRSAESKGKNRFQTATLTHRDIQNHSRYEGKSFGIGGSFDL
                     NGGWDGTVTDKQGRPTDRISLAAGYGSDSDSQSSITKSGINTRNIHITDEAGQLARTG
                     "
BASE COUNT         1778 a         1439 c         1254 g         1054 t
ORIGIN      
        1 aatgtggcag tgaatgctga gggtaaactg gtcaacacag gcatgattgc agcgacggga
       61 gaaaatcatg cggtttcact tcatgcccgc aatgttcata atagcggtac ggttgcctca
      121 caggatgatg ccaatattca cagccagacg ctggacaatt caggtacggt cttatcctca
      181 ggtcaattaa ctgtccgtaa tttaggccgt ctgaaaaacc ataacaacgg tacgatccag
      241 gctgcacgct tagatatgtc aacaggtggt ttggataaca caggtaatat tactcaaaca
      301 ggttcacaag cattggattt ggtatctgcc ggcaaattcg ataacagtgg caagattggt
      361 gtgagcgacg ttccacagac cggtttgaat cccaatccat cagtcatacc acagattccg
      421 agtactgcaa caggttcagg cagcagcact gtctcggcat ctaagcctgg ttcaaacaat
      481 cccgtttcac ctacagcacc tgcaaaaaac cacgccggag gacgcattca aacaacagga
      541 gcatttgaca atgcaggatc aatcaatgca ggtgggcaaa ttgacattgc cgcccaaaac
      601 ggtttggaaa attcgggtag tctgaatgcg gctaaactac gagtatcagg cgattcattt
      661 aacaatacgg taaaaggcaa actccaggca cacgatctgg ctgttaacac tcaaactgct
      721 aaaaacagcg gtcacttatt aactcaaacc ggcaagattg acaaccgtga actgcataat
      781 gccggagaaa ttgccgccaa caatctgaca ctcattcatt cgggccgctt gagcaatgat
      841 aaaaaaggca atattcgagc tgcacattta cagcttgata ccgccggttt acataatgca
      901 ggtaacattc ttgccgatag tggaaccgtt accgccaaga ataatcttcg caatacagga
      961 aaagtttctg ttgcacgact gaataccgaa ggtcagactc tagataatac gcacggacat
     1021 atagaggctg aaacggttaa catccaaagt cagcaactga ctaaccaaag cggccatatt
     1081 actgctaccg aacaactgac tatcaatagt cgaaatgtag acaaccaaaa cggcaaactc
     1141 ctatctgcaa accaagcaca attagctgtt tcagacggcc taaacaacca acatggtgaa
     1201 attgccacca accggcagtt gtctattcac gataaaaatc aaaacacttt ggcgttaaac
     1261 aatgcggatg gcacgattca atctgccggt aatgtatcgc tacaagccaa atcactcgcc
     1321 aacaatggca cattaacagc cggtaacaaa ctggatattg ctttggcgga cgatttcgtc
     1381 gtagagcgcg acctcactgc aggcaaacaa ttaaatctaa gcataaaagg ccgtctgaaa
     1441 aatacccata ccctacaagc aggccatacg ctcaaactca atgccggcaa tatagataac
     1501 caagttacag gcaaaattat tggcggagaa caaacggaca tcacatccga acagcatgtt
     1561 ggcaacaggg gcttgatcaa cagcgacggt ttgacccaca tcggtgcagg tcaaaccctg
     1621 accaacaccg ggacaggcaa aatctatggc aaccatattg ccctggacgc gcaaatactg
     1681 cttaaccggg aagaaacgac ggaaggcagt accaaagcgg gggcaatagc tgcaaggaaa
     1741 cgtttggata ttggagcgaa agagattcat aaccaagaag gtgccctact atccagcgaa
     1801 ggtatttttg ccgtaggtaa tcgactggat gaacaacatc atgcggcagg catggccgat
     1861 acctttgtta atggcagtgc cggtttggaa gtacaaggtg atgcattgat gtccgttcgg
     1921 aatatgcaga atatcaataa tcactttaaa acagagacat acttagccaa agcggaaaag
     1981 caagtccgcg actacaccgt actggggcaa aatacctact atcaggcggg aaaagacggt
     2041 ttattcgaca actcgcaagg acaaaaagac caaactactg ctacgttcca tttaaaaaat
     2101 ggttctcgta ttgaggccaa ccaatggcat gtccgagact accacatcga gacttataaa
     2161 gaacgcatca tcgaaaaccg gccggcacac attactgtgg gcggtgattt gactgcctca
     2221 ggtcaaaatt ggctgaacaa agacagccgg attgtagtag gcgggcgtat tatcactgat
     2281 gatttaaacc agaaagaaat taccaatcaa agtacaacag gcaaaggtcg cacagatgct
     2341 gtcggcacac agtgggattc agttacaaaa aaaggatggt acagcggtag aaaaagacaa
     2401 cgccgtactg aaagaaacca tactccttac catgataccc aactatttac ccacgacttc
     2461 gacacgcctg tatccgtcat ccaacagaat gccgcctccc cttcctttca acccgccgca
     2521 tctgcaatca aactgattga cggagtatcc acggcagccg tcaatggtca gcgcatccat
     2581 accggtaatg tggtctcgtt aaataacgct actgttactc tgcctaacag cagcctctat
     2641 accacccatc ctgacaataa aggctggttg gttgaaaccg atcctcaatt tgcagactac
     2701 cgccgctggt tgggcagcga ctacatgttg caacaactgc aattggacac caatcatcta
     2761 cacaaacggc ttggcgacgg ctactacgaa caaaaacttg ttaatgaaca aatccatcag
     2821 ttaacaggct accgccgact cgacggctac aggagtgatg aagaacaatt caaagctctg
     2881 atggacaacg gccttactgc tgccaaaaca ttcgctctca ccccaggtat cgccttgagt
     2941 gcagagcaag ttgcccgctt aacttcagat atcgtttgga tggaaaatca aaccgtcacc
     3001 ctgtctgacg gttcgactca aaccgtactg gttcctaaag tctatgccct ggcgcgcaaa
     3061 ggtgatctca atacctccgg tggcctgatt agtgccgaac aagtcttact taaactgcaa
     3121 aacggcaacc tgactaacag cggtaccatt gcggggcgac aggccgtact catccaggca
     3181 cggaatatta acagcaacgg caacattcaa gccgaccaaa tcggcttaaa agctgaaaaa
     3241 agtatcaata tcgacggcgg gcaggtacaa gcaggcagac tgctgactgc ccaagcgcaa
     3301 aatatcaacc ttaacggtac aacccaaact tccggtaatg aacgtaacgg caataccgcc
     3361 atcgatcgta tggccggcat taacgtggtc ggaagccata ctgaacaagt agataacaga
     3421 acttcagacg gcatcctatc cctgcatgcc ggcaacgata tcaacctcaa tgcggccacc
     3481 gtctctaacc aagttaaaga cggcactacc caaattaccg ccggcaataa tctcaacctc
     3541 ggcaccatcc gtaccgaaca tcgcgaagcc tatggtacat tagatgacga gaaccatcgc
     3601 catgtccgcc aaagtaccga agtcggcagc agtatccgca cgcaaaacgg cgcactgctt
     3661 agagccggta acgacttaaa aatccgccaa ggcgaactag aggccgaaga aggcaaaacc
     3721 gtccttgccg cgggacgtga tgtcactatc agcgaaggac gccaaataac cgaactggat
     3781 acctcggtaa gcggaaaaag caaaggcatc ctttccagta ccaaaacaca cgaccgctac
     3841 cgcttcagtc atgatgaagc agtcggcagc aacatcggcg gcggcaaaat gattgttgca
     3901 gccgggcagg atatcaatgt acgcggcagc aaccttattt ctgataaggg cattgtttta
     3961 aaagcaggac acgacatcga tatttctact gcccataatc gctataccgg caatgaatac
     4021 cacgagagca aaaaatcagg cgtcatgggt actggcggat tgggctttac tatcggtaac
     4081 cggaaaacta ccgatgacac tgatcgtacc aatattgtcc atacaggcag cattataggc
     4141 agcctgaatg gagacaccgt tacagttgca ggaaaccgct accgacaaac cggcagtacc
     4201 gtctccagcc ccgaggggcg caataccgtc acagccaaaa gcatagatgt agagttcgca
     4261 aacaaccggt atgccactga ctacgcccat acccaggaac aaaaaggcct taccgtcgcc
     4321 ctcaatgtcc cggttgtcca agctgcacaa aacttcatac aagcagccca aaatgtgggc
     4381 aaaagtaaaa ataaacgcgt taatgccatg gctgcagcca atgctgcatg gcagagttat
     4441 caagcagccc aacaaatgca acaatttgct ccaagcagca gtgcgggaca aggtcaaaac
     4501 aacaatcaaa gccccagtat cagtgtgtcc attacctacg gcgaacagaa aagtcgtaac
     4561 gagcaaaaaa gacattacac cgaagcggca gcaagtcaaa ttatcggcaa agggcaaacc
     4621 acacttgcgg caacaggaag tggggagcag tccaatatca atattacagg ttccgatgtc
     4681 atcggccatg caggtactgc cctcattgcc gacaaccata tcagactcca atctgccaaa
     4741 caggacggca gcgagcaaag caaaaacaaa agcagtggtt ggaatgcagg cgtagccgtc
     4801 aaaataggca acggcatcag gtttggaatt accgccggag gaaatatcgg taaaggtaaa
     4861 gagcaagggg gaaatactac ccaccgccac acccatgtcg gcagcacaac cggtaaaact
     4921 accattcgaa gcggcgggga taccaccctc aaaggtgcgc agctcatcgg caaaggcata
     4981 caggcagata cgcgcaacct gcatatagaa agtgttcaag atactgaaac ctatcaaagc
     5041 aaacaacaaa acggcaatgt ccaagttact gtcggttacg gattcagtgc aagcggcagt
     5101 taccgccaaa gcaaagtcaa agcagaccat gcttcggtaa ccgagcaaag cggtatttat
     5161 gccggagaag acggctatca aatcaaagtc agagacaaca cagacctcaa gggcggtatc
     5221 atcacctcca cccgaagtgc cgaaagcaaa gggaaaaacc gctttcagac ggccaccctc
     5281 acccatcgcg acattcaaaa ccacagccgc tacgaaggca aaagcttcgg cataggcggc
     5341 agtttcgacc tgaacggtgg ctgggacggc acggttaccg acaaacaagg caggcctacc
     5401 gacaggataa gcctggcagc cggctacggc agcgacagcg acagtcaaag cagcatcacg
     5461 aaaagcggca tcaacacccg caacatacac atcaccgacg aagcgggaca acttgcccga
     5521 acagg
//
LOCUS       DAWFJW010000120         5384 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFJW010000120 DAWFJW010000000
VERSION     DAWFJW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.120"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            5..274
                     /locus_tag="WGR19_001793"
     CDS             5..274
                     /locus_tag="WGR19_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799273.1"
                     /translation="MRLQADDYLLSSLPPTVTQDGKIIRPERVGEKWVLNGKPVMLSY
                     PKCSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            621..2252
                     /locus_tag="WGR19_001794"
     CDS             621..2252
                     /locus_tag="WGR19_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0799274.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDCVVNKGDDFRSGADFSLSRNPKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVV
                     ATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTH
                     PNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGRHRKERKEGEDGGLLCKFFPDI
                     LACDRLPEPNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFS
                     FENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            2555..2839
                     /locus_tag="WGR19_001795"
     CDS             2555..2839
                     /locus_tag="WGR19_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ0799275.1"
                     /translation="MKLLAALIPLLMSVVGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            2840..4027
                     /locus_tag="WGR19_001796"
     CDS             2840..4027
                     /locus_tag="WGR19_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ0799276.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMAGDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLTE
                     KPESKPIYNGVRQVKTFEYIAGCVQGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVRQSAQQHSDRAQVATLGGKPLQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            4242..5198
                     /locus_tag="WGR19_001797"
     CDS             4242..5198
                     /locus_tag="WGR19_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0799277.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLHQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         1700 a         1115 c         1226 g         1343 t
ORIGIN      
        1 tgatataaga ttgcaggcag acgactattt attgtcgtcc cttcccccta ctgttaccca
       61 ggacggaaaa atcatcaggc cggaaagggt gggcgaaaaa tgggtcttga acggaaagcc
      121 ggtaatgttg tcctatccga aatgttccaa ttttgagcag atcaaacagg gttcttatgt
      181 cggttcgacg gttttaattc tgttcgtagt catttacggc ttcaggcttt tgattaattt
      241 tttaaaagac ataggcaagg ttggtactga ttgatggtta ttgatttttg gtttcttctc
      301 ggtttcttct tggctttgtc tgttgcttgg ctgttttggt aacggttggt agaatcggct
      361 ttttagagtg ttttaaaagg tccgaattat gtttatttct gaatatcatt tagttaaatt
      421 tcaaactgat tcacatattt atagagattt accacaagcg ttaatttatt atagggaatt
      481 gattagaaaa ggggttttta aaacttcgtt ttcatttgat atttttagga atttctttca
      541 tcgttatgat agagatttta tagaaattca attccctgat tcttctacat tattaattaa
      601 attagatgaa gcaaaatgtt atgtttatta tcctagggcg aaatttttta aagattatcc
      661 tatgctttag tttttttgta tctaaatttg cattggcatc agtaaatgct ccgggtaaat
      721 ttgatagggt tgaagtttat gatgatggca gatatttagg tattcgaggt tcagatgaca
      781 aaagaagaag aatttggaaa ggtgtatttg atagagaatc gggaagatat ttaacttcag
      841 aagctcaaga tttaaaagtt aggcatgtat ctactggagc atcaagtacg ggtaaagtta
      901 gttcggttgt atcttcatca gtttcccgcg ctggcgtatt ggcgggggtc ggcaaacttg
      961 cccgcttagg cgcgaaatta agcacaaggg cggttcccta tgtcggaaca gcccttttag
     1021 cccatgacgt atacgaaact ttcaaagaag acatacaggc acgaggctac caatacgaca
     1081 ccgaaaccga caaatttgta aaaggctacg aatatagtaa ttgcctttgg tacgaagacg
     1141 aaagacgtat taatagaacc tatggctgct acggcgttga cagttcgatt atgcgcctta
     1201 tgtccgatta cagcagattc cccgaagtta aagaattgat ggaaagccaa atgtataggc
     1261 tggcacgtcc gttttggaat tggcataaag aagaactgaa taaattaagt tctttggatt
     1321 ggaataattt tgttttaaat cgttgcacat ttgattggaa cggcggagat tgtgtggtca
     1381 ataaaggtga tgatttcaga agtggggctg atttttccct tagccgcaat ccgaaataca
     1441 aagaagaaat ggatgccaaa aagctggaag agattttatc gttgaaagtc gatgccaatc
     1501 cagacaaata cataaaggca accggttatc ccggttattc agaaaaagta gaagtcgcac
     1561 ccggaacaaa agtgaatatg gggcccgtca cggacaggaa cgggaatccc gttcaggttg
     1621 tcgcaacatt cggcagggat tcgcaaggca acaccacggt ggatgttcaa gtaatcccgc
     1681 gtcccgattt aacccctgga agtgcggaag caccgaacgc acagccgctg cccgaagtat
     1741 cgcccgccga aaaccccgca aacaacccga accccaatga gaaccccggc acgcatccca
     1801 atcccgaacc cgaccccgat ttgaatcccg atgcaaatcc cgatacggac ggacagcccg
     1861 gcacaagacc cgattccccc gccgttccgg atcgcccaaa cggtaggcat cgcaaagaaa
     1921 ggaaagaagg cgaagacggc ggccttttgt gtaaattctt ccccgacatt ctcgcttgcg
     1981 acaggctgcc cgagcccaat ccggcagaag atttaaatct gccgtctgaa accgtcaatg
     2041 tagagtttca gaaatccgga atcttccaag attccgcaca gtgtcccgca cctgtcactt
     2101 tcacagtgac tgtgcttgat tcaagcaggc agttcgcgtt cagctttgag aacgcatgta
     2161 ccatagccga acggctaagg tacatgcttc tcgcccttgc ttgggcggtt gccgcctttt
     2221 tttgtatccg cacagtatcc cgtgaagtct agcaggcgca gcaccgccgg gcttcagtaa
     2281 cttgtgccaa ggcaggggga ggacgtccag aaagatttgt aaagacggct ttatcgtctt
     2341 tataaatctt tttggatacc ccttgccgcc ccgccaaaag aacacactct gccgcaaggg
     2401 caggtggtaa ggcgcgcgct ttttgcgccg tccccctgcc cccgcagcgt cgcaagtgag
     2461 actgggggtg tgggggctag tccccgcaaa atctttcaga ttaagaaaca tttttttaat
     2521 gaggcaaccg tgccttttaa gaaagggata gcaaatgaaa ttgttggccg cattgattcc
     2581 gctcttgatg agcgtcgtag gccgtatatt gactgcatta ggcttgatgg cggtaaccta
     2641 ttcaggggtg gatagattgg tagcccattt tcagcaggcg ataaccaata gcataacggg
     2701 cgcgcctcaa gcgatgttgc agctttttta tataagcggc ggtggaaccg ttcttaatat
     2761 cctgtttggt gcgatcgcct ttattctgtc atttaaacaa atgacaaaac tagcaacctc
     2821 aatcgggaag aaaaaataaa tggcagagat ctgtttgata acaggcacgc ctggttcagg
     2881 gaaaacatta aaaatggttt ccatgatggc aggcgatgaa atgtttaagc ctgatgaaaa
     2941 cggcatacgc cgtaaagtat ttacgaacat caaaggcttg aagataccgc acacctacat
     3001 agaaacggac gcgaaaaagt tgccgaaatc gacagatgag cagctttcgg cgcatgatat
     3061 gtacgaatgg ataaagaagc ccgaaaatat cgggtctatt gtcattgtag atgaagctca
     3121 agacgtatgg ccggcacgct cggcaggttc aaaaatccct gaaaatgtcc aatggctgaa
     3181 tacgcacaga catcagggta ttgatatatt tgttttgact caaggcccta agcttctaga
     3241 tcaaaatctt agaacgcttg tacggaaaca ttaccacatc gcttcaaaca agatgggtat
     3301 gcgtacgctt ttagaatgga aaatatgcgc ggacgatccc gtaaaaatgg catcaagcgc
     3361 attctccagt atctatacac tggataaaaa agtttatgac ttgtacgaat cagcggaaat
     3421 tcataccgta aataaggtca agcggtcaaa gtggttttac actctgccag taatagtatt
     3481 gctgattccc gtgtttgtcg gcctgtccta taaaatgttg agcagttacg gaaaaaaaca
     3541 ggaagaaccc gcagcacaag aatcggcggc aacagaacag caggcagtac ttccggataa
     3601 aacagaaggc gagccggtaa ataacggcaa ccttaccgca gatatgtttg tcccgacatt
     3661 gacggaaaaa cccgaaagca agccgattta taacggtgta aggcaggtaa aaacctttga
     3721 atatatagca ggttgtgtac aaggcggaag aaccggatgc acctgctatt cgcatcaagg
     3781 gacagcattg aaagaagtga cggagttgat gtgcaaggac tatgtaaaaa acggcttgcc
     3841 gtttaaccca tacaaagaag aaagccaagg gcaggaagtt cggcaaagcg cacagcaaca
     3901 ttcggacagg gcgcaagttg ccacgttggg cggaaagccg ctgcaaaatc ttatgtatga
     3961 taattggcag gagcgaggaa aaccgtttga aggaatcggc gggggcgtgg tcggatcggc
     4021 aaactgaaga aaacggcaag agagaaaaaa gacccgtaaa ccgtttgaat atagacggct
     4081 tacgggtctt tgtttcgcgc aaagcaaggg ctaaggcagt caggcagcaa atcccgcaat
     4141 gtattaaaac agacgcgtag aaatgccggc tgcctttatc catcctcaaa attgaatatc
     4201 atcctagccg tatcaaggct gtataaataa ggaaaatacc aatgaatata atcgggctgg
     4261 acatctcaaa ggacaccata gacgcaacat tgcataaaac aaacggaagt atccattaca
     4321 ttaaatttaa gaataatgat gatggattaa aacagtttag attgtggata aagggaaaca
     4381 gaatcagaaa agcctatatc ggcatggagg caacaggcat ctattacgaa aaggcagcag
     4441 atatgctttc ttcctactat actgtttacg ttattaatcc cttaaaaatc aaggactacg
     4501 gaaaaagcag gtttaaccgt accaaaaccg acaaagcaga ttcaaacctg atagcagact
     4561 acataaaaag gcatcaagat acattgatac cgtatcagat acccaaaaac aaagcactgc
     4621 aaaaactgat taaccttaaa aatcaattac atcaacatca gaagcaaatt aaaaaccgtc
     4681 ttcatagcac tgaagaagac ttcataagga acatacatca agacttgata gataccatac
     4741 aggacaagat ggaacaggta aaaatagcca tatccgaaca aatcaaaaaa caaacggaca
     4801 ataaccatta ccgcaatctt caaaccatcc cgagcatagg caaagacacc gcatcagttc
     4861 tttatgcgca actgacagaa aaacatttta aaaccgcaaa ccagtttgta tcctatgccg
     4921 gattaagtcc cgccatcata caatcaggga caagcgtaag aggtcggggc agattgagcc
     4981 gatacggaaa cagacgatta aaaagtacgc tgtatatgcc cgccctttgt gcttaccgtt
     5041 ttaacgcatt tccgaaatta ataaataatc tgaaaaaagc gggtaagcca aagatggtaa
     5101 tcatcgttgc catcatgcgc aaactggcga agctcgccta ttacattgtt aaaaccggcc
     5161 agccttacga tgcggaaaga caccgattga atcaataaaa ttcaacaaaa ttaaacggtt
     5221 acgcgaatat atttgtgtaa ccgtgcattt gcatatcgta aataaacgta aataaaaata
     5281 acaatataaa tcagtatatt gcaactttgt tttttatttt gtgttgacgg gcaacatatc
     5341 atctacgcgg gaatgacgaa tccatccata cggaaacctg cacc
//
LOCUS       DAWFJW010000121         5383 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFJW010000121 DAWFJW010000000
VERSION     DAWFJW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.121"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGR19_001798"
     CDS             complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGR19_001798"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0799278.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WGR19_001799"
     CDS             complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WGR19_001799"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0799279.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1964..3205
                     /locus_tag="WGR19_001800"
     CDS             1964..3205
                     /locus_tag="WGR19_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0799280.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSISGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            complement(3275..4927)
                     /locus_tag="WGR19_001801"
     CDS             complement(3275..4927)
                     /locus_tag="WGR19_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0799281.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRAVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQNFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNWELVGNYRNYLYDVLKIAR"
     gene            5097..5383
                     /locus_tag="WGR19_001802"
                     /pseudo
     CDS             5097..5383
                     /locus_tag="WGR19_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226786.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
BASE COUNT         1357 a         1605 c         1240 g         1181 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actataaaat ggaaaatacc cggctatcgt ctcattttcg
       61 ttttaatcag ccataaaaat gccgtctgaa accctttcag acggcatttc tgtcaaacgc
      121 cggacgcact caatccaaac tcaacagcag gttgcggaac gcgttcgggt ctttgataaa
      181 cgtcatctcg cccgcctgcg gaaaatgaaa gtccaacagg cgcgacaccc aaaaacggat
      241 gcagccggca cgttgggcgg tcgggaaata cgccttttct tcggcactca aggggcgcac
      301 gccctcataa ccaccgataa acgccttttt caacgcctca tccaacttat tgtccgctgt
      361 ccttgcccaa tcgttgaccg caatcgccaa gtcatacata aaattgcccc ggcaggcata
      421 atagaaatcg atgaaacccg atacctgacc gccgtcaagc aacacattgt ctttaaacaa
      481 atcggcgtgg atgatgcccg aaggcagatg attgccgaga ttgtccttca gcgcatcgat
      541 ttcggaacac agcagcgcgg catcgtcttg cgacaggacg ggcagcagcc gggtgcacgc
      601 ctccgtccac cacgcatcgt aacgcgggtt ttccatttcc aaagggaaat cggcggcggc
      661 aaggtgcatt ttggccagca tcgcaccggt atggaaacac tgctccgccg tcggcagcgc
      721 ggtatccgaa cctttcaggc aggcaaccaa acaagccggc ttgcccgcca aaacggaatc
      781 aagccggccg tctttgcgcg caaccggcgc ggcaaccgcc acgcccttca tactcaaatg
      841 ccggttaagc tccagaaaaa acggcagctc ttcctgtttc aacacttcaa acacggtcag
      901 cacataacgt cccgaagtcg tcgtcagaaa ataattgctg ttggtaatcc cctgcgcgat
      961 gccctgcagg gaaacaaatt cccccaaatc gtaaccgctc aggaagccgc gcatttcatc
     1021 atcggaaaca ctggtataga cagacatatc aactcacttg ctcaaaaacg ggcaaacccg
     1081 ccgtcagaac gccggacggc aggcaaacat ataattcaca tcggtcgaat cgcacagggc
     1141 ataagtttgc gacaacacat ggtaagtcat acccttcgta tccgccacat ccaagcctgc
     1201 ctgacggcac attcgcgcca gctcggcagg tgcgatgaat tttttccagt cgtgcgtacc
     1261 tttggggaca aacttcaaca gatattccgc cgccacaatc agatgcaggt acgatttcgg
     1321 gtttttattg atggtagaaa aaaacaccat gccgtccggt ttgaccagat tggcacaagc
     1381 acgcacgatg gcggcgggat cggggacgtg ttccatcatt tccatgcacg ttaccacatc
     1441 gaacgagtgc ggttccgcct cggcaaggtc ttccacgcgg atacattcgt attcgatatc
     1501 ggcgacattg ttcaaagccg cgtgcaggcg ggcggtttcc aacgactgct ccgccatgtc
     1561 gatgcccttt acaaacgccg cgccgcgccg cgccatactt tccgccaaga tgccgccgcc
     1621 gcagcccacg tccaaaaccc gtttgccgca caaatccgcg tgtccgtcga tataatccag
     1681 ccgcagcgga ttgatgtcgt gcaaggtttt gaactcgccc gacttgtccc accatttgtc
     1741 ggcaatccgg ctgaatttgg cgatttcccc ctcatcgaca ttatattttt tgtcggacat
     1801 tttccctccc atctgacgaa ccgcccactc caaaacccaa gattatacta tggaacacaa
     1861 tgccgtctga aagccttttc gggcggcgcg gcgcaaatac cttacaaatc cttacacttt
     1921 acggcataat ggcggctcgc tttttctggc agaaagacaa aatatgccca acaaaacccc
     1981 ttcactgttc ggcggcgcga tgattatcgc cggcacggtc atcggcgcag gcatgctcgc
     2041 caaccccacc gccacatccg gcgtatggtt taccggctcg ctggccgtgt tgctgtacac
     2101 ctggttttct atgctttcca gcggcctgat gattttggaa gtcaacaccc actatcccca
     2161 cggcgcaagt ttcgacacga tggtcaaaga cctgctcgga cggagctgga acatcatcaa
     2221 cggcatcgcc gtcgccttcg ttttatacct gcttacttac gcttatatct tcgtcggcgg
     2281 cgacctgacc gccaaaggca tcggcaacgc cttgggcggc aatgtttcac tcaccgtcgg
     2341 acaactcgtc ttcttcggca tcctcgcctt ttgcgtatgg gcatccgcac gcttggtcga
     2401 ccgcttcacc agcgtcctca tcggcggcat ggtattaacc tttatttggg caaccggcgg
     2461 cctgattgcc gatgccaaaa tgcccgtcct gttcgacacc caagcccccg tcggcaccaa
     2521 ctactggatt tacgccgcca ccgccctgcc cgtctgcctc gcttccttcg gtttccacgg
     2581 caacgtctcc agcctgctca aatactttaa aggcgacgcg cccaaagtgg ctaaatccat
     2641 ctggacgggc acactgattg cgctggtaat ttacgtcctc tggcaaaccg ccatccaagg
     2701 caacctgccg cgcaacgagt tcgcccccgt gattgccgcc gaagggcaag tctccgtcct
     2761 catcgaaacc ctgtccaaat tcgcccaaac cggcaatatg gacaaaatat tgtccctgtt
     2821 ttcctatatg gcgatcgcca cctcgttttt aggcgtaacg ctcggactct tcgactacat
     2881 cgccgacatc ttcaaatgga acgacagcat ctccggccgc accaaaaccg ccgcgctgac
     2941 cttcctgccg cccctgattt cctgcctgct cttccccacc ggcttcgtta ccgccatcgg
     3001 ctacgtcggc ctggcggcaa ccgtctggac aggcatcatc cccgccatgc tgctctaccg
     3061 ttcgcgcaaa aaattcggcg caggcaaaac ctataaagtt tacggcggct tgtggctgat
     3121 ggtttgggtc ttccttttcg gcatcgtcaa catcgccgca caggtattga gccaaatgga
     3181 actcgtcccc gtatttaaag gataaaggca aaatgccgtc tgaacgagtt ttcagacggc
     3241 attttcgcaa ccgttgcagc catgcggcaa aaagctaacg ggcaattttc aaaacgtcgt
     3301 acagatagtt gcggtaattt ccgaccaact cccagttcgg gcagtcttcc aaccctccgc
     3361 cgcacaaacc gcgcatcgcc atattcgcac gcatgaagcc gtctttggct tccagaccag
     3421 ccgcctccat cagcacgccg cctgcaaagg caaggtcgag gaaattctgc cgctgtacga
     3481 atccgccggc aatattgctc ctgacggcaa actgcgttac ataatccggc tgcgcgtaat
     3541 cccatttctt cctgacggac acgccctcaa acggtacctg atgatcgccg aaataaccga
     3601 aaacaaaggg tttgccgcgt tcgtgcagat aacggtcgaa actttccacc gctttgtcca
     3661 aatcggcaat gcgcccgatg tagtcgttaa gcgcggatac ggttttcgca ttcaaatcgg
     3721 gcgcatccaa atcaaacaca ttgtcggtat ccgtccgata cggcccgtgc tccttcatgg
     3781 tcagcacata tacgaacatc ggctgccgca cgttttccaa atcgggatgg cgtttttcca
     3841 aaatcatccg cgcatactgc atcatttcct cactggaaat atgccacagg tttttgccca
     3901 tcggcgcggg atagcccaaa tcttgcggct ggaacatcag attaaagccg aaatggtcat
     3961 atgctgcctt ggcattgtag ttgcccttgg taaatggcga gagtgccacg caaaaataac
     4021 cgtgttcgcg caggttgcgg acaaaaccgg tctgcaaatg cggtacgacc gagtaaaaca
     4081 cgccgcttgc caacgcgccg aaatccgtgg acggaacacc cgccaaaaac gcaaattcgg
     4141 acttccacgt tgcgccgccg aaagtatgca cgcgcaaagg tgaggaaaat acggtatctt
     4201 cctgccgtcc gaacattttc aaatcgggaa tcttggcggc ggcaaagtcg aaacagtgcg
     4261 gatcgagcgt cgattccatc agggtaacga caatatcggg tttttcatcc gacatgccgc
     4321 acggccgggt ttcagacggc atctgcctgg caaacgcttc cccgtcgccc tcgaacacgg
     4381 gtacttggaa aaacaccgcc cgacaggaca tcggcaggtt caaaaacacg gcgcgcccgt
     4441 cgtccggcag cgaatccagc cacgtcttta ccgcgcccgg atgtttggaa aaatgccgca
     4501 tcgacacgaa cgccgccgca aacaaaaccg cgcctgccca ccgccacggc acatccaaag
     4561 aatccgcacc gctccagccg aaaaccgcat atgccgccaa agccagcagc cccgccatac
     4621 cgataaccgc ttccttataa tggaacagcg tttcccaatt ccgccagtcg gcaatcagca
     4681 aaaagtcgga aatcaacagc ggctgtttgt aataatggat tttcagcctg tggaacagta
     4741 tcagcaccac aaaccagacc gaagcgaaat tcaaggcacg ttgccactga gccgtcaaca
     4801 tcagcatacc gcccgccaaa aagacaaaca gcgcggatgc gaaaaaatac gtccagcggt
     4861 agtgcgacct gataatcagc accaacgccg ccgttacaaa caaaaacagg aaaacatagg
     4921 caaccatccc gccccactcc tcaaaacggc atacctgagc cgttgcgaat tattatcgaa
     4981 agcgtgcatt ataatgcagc acaggcgcat cactgccgat atttcagtat aattattcga
     5041 gatggaatca ctgaaaatgg tgaagttaca atgagccctt ttagaggaac taaaaagtga
     5101 attataaaat atacgatggt gcaaagatta ttaaaaaaga aaatttatca attggggatt
     5161 attctcaaat tgatgatttt gtatttttta attgtggaga gaaaagtatt ttaggaagtt
     5221 ttgttcatat atcatcattt accagtatta ttggtggtgg agaattttat atggatcatt
     5281 tttcaggatt aagcgcaggg tgtagaatta ttaccggtag cgatgatttt atgggggggg
     5341 gttaaccaat ccaacagtcc catctaaata tactaatgtt aaa
//
LOCUS       DAWFJW010000122         5090 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFJW010000122 DAWFJW010000000
VERSION     DAWFJW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.122"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..565
                     /locus_tag="WGR19_001803"
     CDS             <1..565
                     /locus_tag="WGR19_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799282.1"
                     /translation="ISIFVYKKKIYYLIDYKENFELNAKISFDIYLREGIITKEQYNY
                     DYKNYRNGIWQLTKDNFESYLQSDSVIVLKKDELKELMFQGFTSAEAVRLYSVVENKL
                     SYNDPISDSGRQSDFLKINQISSRLPLFYVNFDTEVYLHMDWDRCHEDYVYDGWFSKA
                     MDFGYLIPDEFCYWKIEGRDYWKFGQL"
     gene            complement(849..1229)
                     /locus_tag="WGR19_001804"
     CDS             complement(849..1229)
                     /locus_tag="WGR19_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0799283.1"
                     /translation="MNIRFFALTVPVLSLAACAVPETYDGGGRGHMPPVQNQARPADF
                     RAFSCENGLSVRVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1705..3786
                     /locus_tag="WGR19_001805"
     CDS             1705..3786
                     /locus_tag="WGR19_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0799284.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3776..3970
                     /locus_tag="WGR19_001806"
     CDS             3776..3970
                     /locus_tag="WGR19_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0799285.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(4166..4357)
                     /locus_tag="WGR19_001807"
     CDS             complement(4166..4357)
                     /locus_tag="WGR19_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799286.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            4486..5046
                     /locus_tag="WGR19_001808"
     CDS             4486..5046
                     /locus_tag="WGR19_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ0799287.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT         1267 a         1374 c         1216 g         1233 t
ORIGIN      
        1 catatctatt tttgtctata agaaaaagat atattacttg attgattata aggaaaattt
       61 tgaattaaat gcaaaaatat cttttgatat ttatctaaga gaaggaatta taacaaaaga
      121 acaatataat tatgattata aaaattaccg taatggtatc tggcagttaa ctaaggacaa
      181 ttttgaaagc tatcttcaat cggattcagt cattgtatta aaaaaggatg aattgaagga
      241 actgatgttt cagggattta cttctgctga agcggtaagg ttatattccg ttgtcgagaa
      301 caagctgtcg tacaatgatc ctatctcaga ctccggccgg caaagtgatt tcttaaaaat
      361 caatcagatt tcctcgaggc tgcctttatt ctatgtcaat tttgacactg aagtatactt
      421 acatatggat tgggacagat gtcatgaaga ttatgtttat gacggctggt tttcaaaggc
      481 gatggatttt gggtatttaa taccggacga attttgttat tggaaaattg aaggacggga
      541 ttattggaaa tttggacaat tataaagata agcaagccat agcttgtatg aattcaaaag
      601 gtcgtctgaa aaacagcttc gacaaagacg cggttgccaa agagatcaac ctacaaaggg
      661 aagtaacgca ggagttcggc agaaacgccg accaagtcgc agcggccgtt gccgacaaac
      721 ttggcaatac ccgaagttac ggacggtatc aggtagccaa aaccctgccg gaggccgaac
      781 tgcaaaacaa atccgacata aaaaaccgcc cgaaaaatcc ggacggcggt tcaaacaggc
      841 tgccccgttt aacgggcgcg gcaggaagtt tcgaccgaat tgccgtaggc atcggtaaag
      901 ccgaaaaagg cttcgccgcc tttctggtgc cactcggttc cgtttccgaa caaaccgtgt
      961 tcggcggtat agcgttcgcc ggatgcggca acgtcggaag agaggacggc acgcctgccg
     1021 tccaaccgca acgcgattct gccgccgtcc aaacggcgga cgcgcacaga caaaccgttc
     1081 tcgcaggaaa atgcccgaaa atctgccggg cgggcttggt tttgaacggg cggcatatgc
     1141 ccgcgtccgc cgccatcata cgtctccggc acggcacagg ccgccagaga caaaaccggc
     1201 acggtcagtg cgaaaaacct gatattcata aaagctcccc aataaaaata agatatgaaa
     1261 caaccgccct gattccaagc tgcggcaacg ccatactata aacggacgcg caaacacaca
     1321 agcccgataa ccggaattta cccgcgatga atcaataatc cggattgcgc gccccttctt
     1381 tacccgtctt ccggcgccgc cttttgcctg acgatgccgt ccaaacccgc ctgtccgccc
     1441 gaaggaatgt aaattttttc caaattccaa gtaaaaaccg ctatcagtgt gctaatttgc
     1501 gttaaaatcc tattcggcgt ttaacgtttt gtgcgcccgc atccctgcac tgtttgatgc
     1561 gggcataagg cacaaatccc gacaagcgca ctgtttcata cttcgtcaat cattcagact
     1621 ccggtttgtg cccgtgccgg caggcggtca tccgtttccc gccgttcagg catattccga
     1681 cagtgtgaga taaggattta ttcgatgaaa tcactcaaaa ccttcctcat ttggggcata
     1741 gtggtactgg tcggcttagc atcctttacc actctggccc tcagccgagg cgaacaggtc
     1801 agcgcggtat ggatggttac cgccgccata tccgtttact gcatcgccta ccgtttttac
     1861 agcctctaca tcgccaaccg cgtaatgcgg ctcgatccta accgcctgac tccggcagaa
     1921 cgccacaacg acggcttgga ctacgttccg acgcacaaag gcgtattgtt cggacaccac
     1981 tttgccgcga ttgcgggcgc gggccctttg gtcggcccgg ttttggcggc gcaaatgggt
     2041 tacctgcccg gtactttgtg gattatcttc ggcgtggtgt ttgccggcgc ggtacaggat
     2101 atgatggtac tgtttgtttc tatgcgccgt gacggtaagt ctttgggcga tattgtgaaa
     2161 caggaactcg gcactgtccc cggcgtgatt gcatccatcg gtattttgat gattatggtc
     2221 atcattatgg cggtgttggc attgatcgtt gtgaaagcat tggttcacag cccttggggt
     2281 acgtttacca tcgccgccac tatgccgatt gccctgttta tgggtattta cacgcgctat
     2341 atccgtccgg gcaaaatcgg cgagatttcc atcgtcggct ttattttgct gatgctggcg
     2401 gtaatttacg gcgaagatgt ggcgaaaagt tccatcggac attggttcga ccttgacggc
     2461 atccagctca cttgggcgat tatgatttac ggctttgtcg cctccgtatt gcccgtatgg
     2521 ttgctgctca ctccgcgcga ctatctctct accttcctga aaatcggtac gattgcggcc
     2581 ttggctttgg gcatcgtcat cgtcaatccc actttgcaaa tgcctgccgt aacccacttt
     2641 atcgacggtt cgggtccggt attctcaggc gcattgttcc cgttcctctt catcaccatc
     2701 gcctgcggtg cggtttcggg cttccacgcg ctgatttcct ccggcaccac gccgaaaatg
     2761 ctggaaaacg aaacccacgt ccgtatgatt ggttacggcg gtatgttgat ggaaagtttc
     2821 gtagccatta tggcgcttgc cgccgccgca tcgcttgatc ccggcgtgta cttcgccatg
     2881 aacagccctg ccgccctgat cggtacggat gccaacaccg ccgccgaagt gattaccacc
     2941 aagctgcaat tccctgtcga tgcggcaacc ctgttgcata ccgctaagga agtgggcgaa
     3001 aacaccatcc tttcccgtgc aggcggtgcg ccaaccctcg cagtcggtat ggcgcacatt
     3061 atgagccgcc tgattccggg cgaggcgatg atggcgttct ggtatcactt cgccctattg
     3121 tttgaagcct tgttcatcct gaccgccgtc gatgccggta cgcgcgtcgc acgttttatg
     3181 attcaagact tgggcagcat cttctacaaa cctttcggca acaccgactc catccccgcc
     3241 aacctgattg cgaccttctt cgccgtggca ttgtggggct acttcctcta caccggcgta
     3301 accgacccgc tgggcggcat caactcgctc tggcctttgt tcggcatcgc caaccaaatg
     3361 ctggcaggcg tggccttgat tatgtgcgcc gtggtgctga ttaagatgaa acgcgaccgt
     3421 tatgtttggg tggtactcgt tcccgccgtc ggcgtactgt tcgtaacctg ctacgccggc
     3481 ctgcaaaaac tgttccacag cgacccgcgc atcagcttcc ttgcccacgc cggcaaatac
     3541 agcgacgcat tggctaaaaa cgaagtcctt gcgcctgcca aagacatcgg cgaaatggcg
     3601 caaatcatct tcaacgacaa gattaatgcc ggtctgacca tcctcttctt gtcggttgtc
     3661 gtgattgtcg ccgcgtacgg tttgcgtacc gccctcaaag cacgcaaagt cggctggccg
     3721 accgccaaag aaatcccggc ggtgtaccgc gacggcaagc agccggaggc acaaagtgaa
     3781 gcataagctc gcgtcttggt ggaaaaccat caagctgacg gcaaacttga tggcaggcgt
     3841 gcctgattat gaaaactacg ttgcacagca gcgcaaacat aatcccaacg cccccgtgat
     3901 gaccaagctg caatttcaag actactgccg caagcgccgt tgcggcgcaa acggcggacg
     3961 ctgctgttaa gcctgcttga aacaaattcc gtctgaacgc cgcttcagac ggaattatta
     4021 taatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     4081 tacgaaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagccaagg
     4141 cgagacaacg acgtaccggt ttttgttaat ccgctatacc acgatgaatc cttcgcaata
     4201 tctgtttatc gacctcaatt ttgacaaaat accggatacg cgcctttgtt gcttttccat
     4261 cttccaacca actgtaaatc tcaaacagcc ggtacacgcc atgcttcagt ttcttttcct
     4321 gtcggcggat tgtttcgaca aagaattgaa aatccatttc atgcacctta aaatttaatc
     4381 tgcattcaaa ccttttcact ttggaagcac catttatcgg atgtcccttc gcaataaaca
     4441 aattttcccg ataccgccgc ccatttcaac ccaaacccaa aacctatgaa aaacctcatc
     4501 gccttcaaca aaccctatgg cgttatctgc caattttcac cgcacgaaaa acacaaaagc
     4561 ctcaaagact ttatcaatct tcccggcttc taccccgccg gacggctcga caccgacagc
     4621 gaggggctgc tgctgctgac cgacgacggc aggcttcagg cacaaattac cgaccccaaa
     4681 ttcaaacacc ctaaaaccta ctgggcgcaa ctggagggcg tacccgacga aagccgattg
     4741 gaaagcctaa gaaaagggat agacttaggc ggtttcgtta cccgtccggc aagcatccgc
     4801 atcttgaaac acggagaagc agattcgtta tgggagcgca tcccgccgat acgcgtccgc
     4861 aaaaccgttc ccgatttttg gattgaaatt accatttctg agggcaaaaa ccgccaagtc
     4921 aggcgaatga ccgccaaggc gggctatccc tgcctgcgtc tgatcagagt ggcaagcggc
     4981 aggctgaaac tgtttgattt ggatttaaaa cccggggaat gggcatacgc cccgtttaaa
     5041 ccataatcac gtttatctca tcatttccac aaaagtggga atccggaatt
//
LOCUS       DAWFJW010000123         5039 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFJW010000123 DAWFJW010000000
VERSION     DAWFJW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.123"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(78..1415)
                     /locus_tag="WGR19_001809"
     CDS             complement(78..1415)
                     /locus_tag="WGR19_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0799288.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDSIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALSVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
     gene            complement(1802..2866)
                     /locus_tag="WGR19_001810"
     CDS             complement(1802..2866)
                     /locus_tag="WGR19_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0799289.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(2867..3625)
                     /locus_tag="WGR19_001811"
     CDS             complement(2867..3625)
                     /locus_tag="WGR19_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0799290.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     VEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(3700..3775)
                     /locus_tag="WGR19_001812"
     tRNA            complement(3700..3775)
                     /locus_tag="WGR19_001812"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3740..3742),aa:Ile,seq:cat)
     gene            complement(3829..4965)
                     /locus_tag="WGR19_001813"
                     /pseudo
     CDS             complement(3829..4965)
                     /locus_tag="WGR19_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(4910..>5039)
                     /locus_tag="WGR19_001814"
     CDS             complement(4910..>5039)
                     /locus_tag="WGR19_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0799291.1"
                     /translation="ADRQNHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
BASE COUNT         1273 a         1252 c         1296 g         1218 t
ORIGIN      
        1 aacgaaatag gctgaacccg aagcaaaaat gccgtctgaa aggctttcag acggcatttt
       61 tgctgtttgg atggctttta aaggatgtcc aaagaattga tgaaaacaat aataatggca
      121 atcggggcaa ggtagcgcaa ggtattcagc cacagcagca gcactgcccg tggtacggtg
      181 ctgcccgcgc cggcatcttt taacacagac tgcttgtcct gaatccaggc ggtaaatacg
      241 gcaacgctca atgcgccgac aggcataatg acggcggaaa taacatagtc ccacaaatca
      301 aaaatggttt tgccgaaaac cttaaactca ccccatacgc cgaaagacag cgcggatggg
      361 atgccgacaa tgaaaatggc cgtgccgata agccaagtgt gttttttgcg tttgcgctcg
      421 tcttggcgga tggttgcggc gatgaccgtt tccaacatcg aaaatgccga agtcagcgtg
      481 gcgaaaacga ccagcagcat aaataccgca aacaaaaccg taccgaacgg cattttcata
      541 aacaccgccg gcaatacgat aaaaatcaat cccggcccct ggctcggttc aaaaccgaag
      601 gcgaacaccg ccggaaaaat caccaaacct gccaacagcg aaaccaagag gttcatccac
      661 ataatcgtat gccccgagcg gaacatatcc tgatcttttc ccaaatacga agcataggta
      721 atcatagcgg aaacgccgat gctcaaggca aaaaacgcct ggcctaaagc cgtaatcatc
      781 gtatcggctt taaagtacga ccaattcggt ttgagcagga aagacacgcc ctccattgca
      841 cccggcagcg tcagcgaacg gattgccaga gcaataaaga ggataaacag ccccggcatc
      901 agataacggt ttgccttttc aatgctgtct gaaatgccgc ctttgaccac ccaaaccgta
      961 atcagcataa acagtgcctg ataggacagc gaacctgccg gattggaaat cgtcgcgccg
     1021 aacaaggctt caaagtccgc gccggtatga accgcccccg tgaaactgtg gacgacataa
     1081 tttaataccc atccgccgac cacgctgtaa aacgacagca aaataaagca ggcggaaacg
     1141 cccatgcgcc cgacccaaag ccattgcgta ctcggacgca gaaccctgaa ggaatcgacg
     1201 gcatttttac cgcccgtgcg cccgatataa aattcggcaa gctgaacggg tagggcgacc
     1261 aagatagtaa atatcaaaaa cagcaggaaa aacaccgcgc cgccgttggt gcctgccgta
     1321 taaggaaatt tccaaatcgc gcccaaaccg atggccgaac ctgccgcagc aaggacgaaa
     1381 ccgattttgg acgaccaaga agtatggtta ctcataaaat ttattaagaa atgtgaaggt
     1441 agtatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt
     1501 acggaaccga ttcacttggt gcttgagcac cttagagaat cgttctcttt gagctaaggc
     1561 gaggcaacgc tgtactggtt tttgttagtc tactatacat tgtaacaaag cacttgacgc
     1621 ggcagcacaa gccggggaag acgctgcaaa actttgccaa taggctgcgt aatatcgggg
     1681 gatattggga ttttcttatt ttgaaggatg aattcggggt gtttctttcc aatgaacgaa
     1741 tataatttag tggtttgttg catcaaaggc tttcagacgg catatgccgt ctgaaagcag
     1801 tttatattgc atccaataat ttgtcggcgg tatggttgat ggtaaaacgg gcggcgacgc
     1861 attgatgagc tgccgttgcc agtgcgtttt tcgtttcagg gttcaagacg aggcgttcga
     1921 tttcgtccgc caaagattca ggtgtcagcc tgtccagcag aatccccgat tgatgatgtt
     1981 cgacaatttc cttttgcgcc cccaaagtat tggaaatcac cgccgttcgg cagtacatcg
     2041 cctcgcataa tgacaaaccg aatgcctccg ggacgaggct gggcaaaacc acgacatcgc
     2101 tttggcggta aaacgaagca agtttttcgg taaacccttc aaaagaaaca aatggttctg
     2161 ctcctgaagc agatacgtcc cgcttcaggc ggcacatata atccggatgt ccgtcccctg
     2221 ccaatttgag cctgatttga ggatatttcc gatgcagtat cacacaggct tcaatcaggt
     2281 tttccaatcc tttttctgga ctgatcctgc cggcgtaggc gacggtaaaa aaacggctgt
     2341 cgggtttttc ttgagaggga gggaaacggc cggtatcgat accgttatga acaatccggt
     2401 atttttcttt aaagggattg tcggcggttt gcacatcgta aaccagacgg gaaacgcaaa
     2461 taaagcggtc tgtgtttttc tgtatcaggc ggtggtaaaa atcggttttg ttggcgacga
     2521 cattatgttt gacaaatatc aggcgcaccc cggtcagttt tttcaaaagt atggataagg
     2581 cggcaatttt gccggtgtgt atcatcaggt gggaaatgcg gtttttccgg ataaagcggg
     2641 taagtgcgta gagcgaaaac agcccgttga gcgtgtgaag gcgcgttgtg aaaacggaag
     2701 aaacttcgga aaatcgcctg tgcatcaatt cattattttt attgacggcg gtaaacattg
     2761 tgcagccccg aagccccaat gcttttgaaa catcatagac atattgttcg cctccacccc
     2821 aaatcttggc cgatacgata tttaatatcc tgaattcttt ttccatttaa aattttccgc
     2881 cggatttgta cagataatat agtttgacat atttaatcat cgtgtaatag ctgtggttga
     2941 cggacataat ccaacccatt tttccatcaa gaaacccttt gttcaggata taaattttga
     3001 aaaaccccca aatcgggcgg aggataatgt ccctaacgaa acgcacgggc tttccctgct
     3061 ctcggtattt ttcggctgaa atggaagtat atttgttgaa cttgttgaaa tattgttccc
     3121 agttgtcgta cgtgtaatgg tacataaaat gcttcagacg gcgtttgggg tagggggttt
     3181 gtacggtttc gtgtactttg ccttgcaccg aactgtcttt tttcggcatc agacggcata
     3241 cgctgtcggg acgcatcgcg ccgtgtgtgg cgggatggtt ggggaaaagg ttgcggcgtt
     3301 cgacaaaata ggcggcataa tcgccggttt ggacaatttt tgagatttca tcagatagtt
     3361 ccggcgtgca gcgttcgtct gcatcaatca ggaaaaccca ttctccgcct gcctgttcga
     3421 tggcaaatgt tttttgcgct ccgaaatccc cattcaaatg ccgtctgaag acttttgcgc
     3481 ccaaaccctc gacaatttcg gcagtattgt cggtgctgta gtcgtcgata acgataactt
     3541 ctttatcgaa acggcaactt tcaatacatt cctgaatgtg gtttgcttcg tttttggcaa
     3601 caatcaatac gctgactttt ttcattatca aggtttctca aagcattttg gtttcacagg
     3661 gcggcaaaaa agcccaaaca tacgtccggg cttgggaatt ggtgggtccg gtggggttcg
     3721 aacccacgac caagggatta tgagtcccct gctctaaccc ctgagctaca gacccgtcaa
     3781 gagtaggcgc gtattgtata gcatagcggt gggattgaca aacttatttc atccgatgat
     3841 taagacgcag ttgcttcctc cgaaggcaaa cgacgagctg gcggtaatgc gtttttcggt
     3901 ttcccagcgg ctgccgctgc cggtcaggtt gatggcggga aggttcggat cgttctgccc
     3961 gtcccaaagc tggggcggaa gttttccttc gggattgctt tgccggtcgg caatgcccca
     4021 cgcgaacgcg gcttcgattg cgcccgccgc acccagcgtg tgtccggttt gcggcttggt
     4081 ggacgtgcag ggcgtattgt tgccgaaaac cgctgcaacg gcacggcttt ccatactgtc
     4141 gttgtggtgc gtcccggtgc cgtgcaggtt aatccagccg atgtcttcgg gcgcaaggtt
     4201 tgcgtgttgc aatgccgtct gaaaggcgag gattgcgcct tgagcgtcgg ggcgcggcgt
     4261 ggaaatatgg taggcatcgc tgttggctcc gtagccgacg agttcggcgt aaattatcgc
     4321 accgcgccgt ttggcgtgtt ccaattcttc cagcacgaaa atccccgcgc cttcgccgat
     4381 gaccagcccg tcgcggttcg cgtcgtatgg gcgcggggtt ttttccggtt cgccgttgcg
     4441 gcggctggcg gcataaagcg agtcgaaaac atacacttcg gacgggaaaa attcttcgcc
     4501 tccgcccgcc agcatcatat cggtcagacc gtatttgatg gcttcgtagg cataacctat
     4561 gccttggctg ccggacgaac acgcgctcga tgtcgggatg atgcgtcctt tcagcccgaa
     4621 aaagatgccg atattggcgg cggcggtgtg cggcatcata cgcacatagg tgttggcact
     4681 gaagttgcgc gacgtgccgg tcagcaacaa ttcgcccaca tcgccgatgt ctttggtgct
     4741 gccgctggaa gagccgcagg caacgcccat gcgtccgtca gtaatgcttt cgtctccgag
     4801 caaaccggca tccgtcaaag cctgctccgc cgcatcgacg cacaaatacg acacacgccc
     4861 catacttctg agctgcttgc gcgtccaatg tttcggcggc gcgtaatcct caatcggcgc
     4921 acccagttgc gcttccaatt cggggaaacg ttcgtgccaa tccactatat tttcgcaaga
     4981 cgcgttttgc ctgattccaa aaattctcaa tgccgttaat gtggttctga cggtctgca
//
LOCUS       DAWFJW010000124         4972 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFJW010000124 DAWFJW010000000
VERSION     DAWFJW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.124"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..2232)
                     /locus_tag="WGR19_001815"
     CDS             complement(<1..2232)
                     /locus_tag="WGR19_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0799292.1"
                     /translation="MRHSHYFQWLSLPLLSVAVTQQLYAQPNESLPTVELEPVVITID
                     KSGMALANRITQMPHTTKVIYEEQIQEQATGSRQLADVMAQLIPSLGVSSGTTSNFGQ
                     TMHGRQVQFLLNGVPLTGSRDISRQLNSINPNQVARIEVLSGATSIYGSGATGGLINI
                     VTKSDLEEGQFETRIGVHGSKLSNEGIGYQVGQSVAGVSENGNVLTRLDVDYRTTGGA
                     FDASGKRIAPEPAQTDKQDSKSLSVNANVDWQIDDKQNINLALTHYSDKQDTKYAPDY
                     GNGLSVLFGAAPSLNAVEGLSLSEQPKTTKSTFNINYHHDDLWGNTINTNAYYRKEEG
                     RFYPFVAPFSVDKALPVLQSMNLPSATLDALQARAYGVLQSESKADVLGARLALQRKF
                     DLAQKPVLLSYGIDYEQEKDRQGATGYDLTTFMQSNGLAFKDTGNRYTYGPDTTLKTY
                     GIFANANVALTDDLKISAGVRHQIIDSKTDGFVPPSESLLADLLNQNKVPYQAGVVNS
                     GKVKHHKTLFNLGANYQLTPNHSIFANFGQGYSLPDLQRVLRDVGTGFAVNSDNVEPI
                     SVNSYDVGWVGTFGDSHAKLTAFYNTSDKVTQFLKDYSVVVADTDERIYGAEFELNHK
                     INDSWTMGGSIAYTRGQYKDASGQYRELGAFRISPTKATAFAQYTTPNEHKLRLQLLA
                     IDGTQRAYKDSLIAKTNQNVRKTPEARISGYVVADLLGEIKLPKGQLNFGIYNLTNKK
                     YKSV"
     gene            complement(2279..3535)
                     /locus_tag="WGR19_001816"
     CDS             complement(2279..3535)
                     /locus_tag="WGR19_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0799293.1"
                     /translation="MIISQQKNRHLLTMVALLYVAHALPLYFFNVALPAILRHQGVDI
                     RVIGMLSLLYLPWAFKFLWASYVDNYYCAKIGKRKTWLFFTQIIVVVGLFGLGFLQFS
                     ESEIVLFLSISFLVSLASATQDIAIDGYTVEQFDKSSFGLASAMQSFGVAFGSMVGGA
                     GTLWLYQRFDWQMATISLATTVVLISCSLWFLKDQDNPKHATNISKASLIRLIKRPQT
                     KWLLALIVVFRFVEAPMMAMLNPMLVDFGFSLSQIGLLFSVFGAIVGIGSALLAGVLI
                     KYIDIVRCLILFGWLRTAVYALLCLGLLYPLLGDFLPAVGLLKTGFMSLMAISVLLIL
                     SVRYLTMTALYALFMNAANPDQAGTDFTLFVCVELLVYFIGGAMSGFLVHQFGYDKLF
                     LWLAIGSGVSMLILPTLIKKFSVTDC"
     gene            complement(3535..3933)
                     /locus_tag="WGR19_001817"
     CDS             complement(3535..3933)
                     /locus_tag="WGR19_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799294.1"
                     /translation="MPIFLNAIQNPNSSLPVFLLDFSGEISQDAFDDWLIAMSDLLAS
                     EQSFALIYHSRTGLTLPNNYRQIEAVWYKTHKESFFKYCKGLVRIVDDETQRQALDSP
                     AFHKAWRVPYAVVLSLTEAKAWINTKIGAV"
     gene            complement(3938..4540)
                     /locus_tag="WGR19_001818"
     CDS             complement(3938..4540)
                     /locus_tag="WGR19_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0799295.1"
                     /translation="MPTLSQGLPNNYIYRENGVEFGLVQIRYPEHIELLHRWMNMPHV
                     IPQWQLNKSLSELSVYFEKMLADDHQRLYLVSINGEWVGYAEVYEGARDRLSGYYTAD
                     KNDLGWHLLLAEAQAVGKGYLRAIMRLLSFFIFEHSPAKKIVGEPDASVKPYEVVAQE
                     LNYMPQYQIVMPEKTAMLYFCDKEGFYCKFAHYLNASIKE"
     gene            complement(4725..>4972)
                     /locus_tag="WGR19_001819"
     CDS             complement(4725..>4972)
                     /locus_tag="WGR19_001819"
                     /inference="COORDINATES: protein motif:HMM:NF018031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799296.1"
                     /translation="SEFEFWNELSKIIDEFHHQHPELNERIALFDLKRPKFEKVCLNR
                     VRFFTRGYQDNANRPEPVVCEPICNPISPQFLRCVEH"
BASE COUNT         1548 a         1093 c          893 g         1438 t
ORIGIN      
        1 tacactttta tattttttat ttgtcaagtt ataaatccca aagtttaact gacctttagg
       61 tagtttgatc tcacccaata aatccgccac gacatagcct gaaattctag cctccggggt
      121 tttgcggaca ttttgattgg ttttggcaat caggctgtcc ttgtaagctc tttgtgtacc
      181 atcaatggca agtagttgta agcgtaactt atgttcgttt ggcgtagtat attgagcaaa
      241 agcggtggct ttggtagggc tgatacgaaa cgccccaagt tctcgatatt gaccgcttgc
      301 atctttgtat tgcccccgtg tataggcaat actaccgccc atggtccagc tatcgttaat
      361 tttatggttt aattcaaact ctgctccata gatacgttcg tcagtatcgg caacaaccac
      421 gctataatct tttaaaaact gagtgacttt gtctgatgta ttataaaatg ctgtcagttt
      481 agcatgacta tcgccaaatg ttcccaccca acccacatca taactattga ctgaaattgg
      541 ctcaacatta tcagaattaa ccgcaaaacc cgtcccaaca tctcttagca ccctttgtaa
      601 atcaggcaaa ctatatcctt gcccaaaatt ggcaaaaata ctgtgatttg gcgttaattg
      661 ataatttgcc ccaagattaa acaaagtttt gtgatgcttt actttgccag aattgacaac
      721 ccctgcttga tacggcactt tattctggtt taataaatcg gctaatagcg actcactggg
      781 tggcacaaaa ccatctgttt tactgtctat aatttgatgc cgtacacctg ccgatatttt
      841 taaatcatct gtcaaagcaa cattagcatt ggcaaatatg ccgtaggttt ttaaggtggt
      901 gtcaggtcca taagtgtaac ggttgccagt gtctttaaat gccaagccgt tgctttgcat
      961 aaaggtagtc aaatcataac ctgttgcccc ttggcggtct ttttcttgct cataatcgat
     1021 accataacta agtagtacag gtttttgagc aaggtcaaat tttctttgca aagccagtct
     1081 tgctcccaat acatctgcct tggattcgga ttgtaacacc ccataggcgc gagcttgtag
     1141 agcatccaaa gtggctgatg gcaaattcat gctttgtaaa acaggcaatg ctttatcaac
     1201 cgaaaatgga gcaacaaagg gataaaatcg cccttcttct ttacgataat aagcattggt
     1261 attgatggtg ttaccccaca aatcatcatg atgatagttg atattaaagg tgcttttagt
     1321 ggttttaggc tgttctgata aagacagtcc ctcaactgca tttaaagatg gtgctgcccc
     1381 aaatagcaca gaaagaccat tgccataatc gggagcatat ttggtatctt gcttatccga
     1441 ataatgtgtc aatgctaaat tgatgttttg tttatcatcg atttgccaat caacatttgc
     1501 attaacactt aggcttttgc tgtcttgttt gtcggtttgg gcaggctcag gagcgatgcg
     1561 tttaccgcta gcatcaaatg cccctcccgt ggtgcgatag tcgacatcaa gtcgtgtaag
     1621 gacattgccg ttttcgctga cacctgccac actctgacct acctgataac cgatgccttc
     1681 gttggataat ttactaccat gtacaccgat gcgggtttca aattgaccct cttccaaatc
     1741 cgacttagta acgatattaa tcaaaccgcc tgttgctcca gacccataaa tactggttgc
     1801 tcctgataaa acttcaattc tagcaacttg attgggattg atactattaa gctgtctaga
     1861 gatgtctcgc gaacctgtca aaggcacgcc atttaacaaa aattgcactt gacgaccgtg
     1921 catggtttgc ccaaagttac tggtagtgcc actacttacc cccaaacttg gaatgagctg
     1981 tgccatcaca tcggcaagct gtcgagagcc tgttgcttgc tcttgaattt gctcttcata
     2041 aataactttg gtggtatggg gcatttgcgt gatacgattg gcaagtgcca taccgctctt
     2101 atcaatggta ataaccacag gctctaattc aaccgttggt aatgactcat tgggttgagc
     2161 gtacaactgc tgagttactg ccacacttag taaaggcaaa gataaccatt gaaaatagtg
     2221 ggaatgacgc ataaattact ccttatggaa aagtaaaatt ttaacagtct gttacggttt
     2281 aacagtctgt tacggaaaat ttttttatca atgtcggcaa aataagcatt gacaccccag
     2341 aaccaatcgc aagccacaaa aatagcttgt catagccaaa ctgatgtact aaaaatccac
     2401 tcatcgcccc gccgataaaa tacacaagca actccacgca cacaaacaaa gtaaagtccg
     2461 tgccagcttg gtcgggattg gcagcattca taaacagagc atataaggca gtcattgtta
     2521 aatagcgaac agataaaatt agcaaaacac taattgccat caagctcata aaccctgttt
     2581 ttaataaacc taccgcaggt aaaaaatcac ccaataaagg atatagcaat cctaaacaca
     2641 acaaagcata aactgccgtt cttagccatc cgaataatat aagacaacgt acaatgtcga
     2701 tatatttgat taatacccct gccaaaagtg ctgaaccaat cccaacaatc gctccaaata
     2761 ccgaaaacaa aagcccaatc tgagacagtg aaaaaccaaa atccacaagc attggattta
     2821 gcattgccat catcggagct tccacaaagc gaaacaccac aatcaaagct agtaaccatt
     2881 tagtttgcgg gcgtttgata agcctaataa gacttgcttt ggaaatattg gtagcatgtt
     2941 tgggattgtc ttgatctttt aagaaccata gtgaacacga aataagcaca acagttgttg
     3001 ccaaactgat tgttgccatt tgccaatcaa atctctgata tagccacagt gtgccagcac
     3061 cgcctaccat actaccaaac gccactccaa aactttgcat ggcacttgcc agcccaaagg
     3121 atgacttatc aaattgttcc actgtatagc catcaatagc gatatcttgg gtggcacttg
     3181 ccaaagacac caaaaaactt atgctcagaa ataatacaat ctcagactca gaaaactgta
     3241 aaaagccaag cccaaacagc ccgactacca cgataatttg agtaaaaaat aaccaagttt
     3301 tgcgtttgcc gattttggca caataataat tgtccacata acttgcccac agaaatttaa
     3361 acgcccacgg caaatataaa agtgaaagca tgccaatcac cctaatatca acgccttgat
     3421 gtcttaaaat ggcaggcaat gccacattaa aaaaatacaa aggcaaggca tgggcaacat
     3481 ataacagtgc caccatcgtc aaaagatggc gatttttttg ttgtgagata atcatcagac
     3541 cgcccctatt ttagtgttta tccatgcttt ggcttcagtc agtgacaaca ccaccgcata
     3601 aggcactcgc catgctttat gaaaagcagg actgtctaag gcttgtcttt gagtctcatc
     3661 atcaacaatt ctgactaatc ccttgcaata tttaaagaac gattctttgt gcgtcttgta
     3721 ccaaaccgcc tcaatctggc ggtagttatt agggagtgtc agccctgtcc gactatggta
     3781 aatcaaggca aaactctgct cactggctaa caaatcactc atagcaatca accaatcatc
     3841 aaatgcatct tgactaatct cgcctgaaaa atcgagtaaa aatacaggta aggagctatt
     3901 tgggttctga atagcattta gaaaaatagg cataacatca ctctttaatg ctggcattta
     3961 aataatgggc aaacttacaa taaaaccctt ctttgtcaca aaaatataac attgcagttt
     4021 tttcaggcat tacaatttga tattggggca tatagtttaa ttcttgagcg actacctcat
     4081 agggttttac acttgcatca ggttcaccca caattttttt agcaggtgaa tgttcaaaaa
     4141 taaaaaagct tagtagacgc atgatggcac gcaagtaccc tttgccaaca gcctgtgcct
     4201 ctgctagtaa caaatgccaa cccaaatcat ttttgtcagc tgtataatac cctgatagtc
     4261 tatcccttgc cccttcataa acttcggcgt aaccaaccca ttcgccatta atactaacca
     4321 aatacaaacg ctggtgatca tcagccaaca ttttttcaaa ataaacagac agctcagaca
     4381 atgatttatt gagttgccat tgagggataa catggggcat attcatccaa cgatgcaaca
     4441 attcaatatg ttcagggtag cgtatttgca ccaacccaaa ttcaacccca ttttctctgt
     4501 aaatatagtt gtttggcaac ccctgagata aagttggcaa tcgattgatt ttactgcatt
     4561 gatcttgatt aaaagaatcg gtatagattt ctagcatttc tattctctca ttagggtgtg
     4621 ttgaatattt ggtatttgca atgaaaaatg agaaaaatcg cacgtttttc atgcaaaaac
     4681 taataaaagc gttaaaattt tcatcatttt tacaaataat gttatcaatg ttcaacacac
     4741 cttagaaact gtggtgatat tggattgcaa attggttcgc aaaccacggg ttctggacgg
     4801 ttggcgttat cttgataacc tcttgtaaaa aagcgaacac gatttaggca caccttttca
     4861 aattttggtc tttttaaatc aaataaagca atcctctcat ttagctctgg atgttgatgg
     4921 tgaaattcat caataatctt tgataactca ttccaaaatt caaattcaga ta
//
LOCUS       DAWFJW010000125         4941 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFJW010000125 DAWFJW010000000
VERSION     DAWFJW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.125"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(154..1092)
                     /locus_tag="WGR19_001820"
     CDS             complement(154..1092)
                     /locus_tag="WGR19_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0799297.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLALLALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQNAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1101..1976)
                     /locus_tag="WGR19_001821"
     CDS             complement(1101..1976)
                     /locus_tag="WGR19_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0799298.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2158..2234)
                     /locus_tag="WGR19_001822"
     tRNA            complement(2158..2234)
                     /locus_tag="WGR19_001822"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2198..2200),aa:Met,seq:cat)
     regulatory      2384..2483
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2576..4477
                     /gene="thiC"
                     /locus_tag="WGR19_001823"
     CDS             2576..4477
                     /gene="thiC"
                     /locus_tag="WGR19_001823"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0799299.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1206 a         1378 c         1305 g         1052 t
ORIGIN      
        1 tgttgggttc tagattcccg cttttgcggg aatgacggca gggtggtttc tgttgtttcg
       61 gataaatttt gcagccctga taaaaaatat ggctgctttg gtaaaaaatg ccgtctgaaa
      121 ggttttcaga cggcattttg tttttaagaa gcatcagcgg aagcggacga tttccctgtc
      181 ttcgatatgg atgcgtaccg tatcgtttcc cgatatttca ccggcgtgcg gcatatcgag
      241 gttcagccac aggatgccgt gttccggatg gaggacggac aggctgaacg attcgggcaa
      301 acaggtacgg gataatacgc ggcactccat gccgtcttgg tcgaaacgca ccgcattttg
      361 cggaatatgg cggtcatcgt cggtattggg caaacccatc agtcgggcga cctgcacgcc
      421 ggcaggcgtt tgaaccaagg tttcgggcgt accgcattga aggattttcc cctcgtgcat
      481 gacggcgatt tcgtccgccg ccgtgcaggc ctcttcgggc gaatgcgtta ccaaaacggc
      541 agggatgccg ccgtttcgga tacgttcggc agtcatgcgg cgcagccggt cgcgcaaatg
      601 cgtgtccaaa ctggaaaacg attcgtccag caacagcaag gaagggcgga caaccaaagc
      661 gcgcgccagt gccaaccgtt gcttctcgcc tccggaaagt ttttcaggct tgcggtgcgc
      721 ctcattttcc agtccgactt cggcaagtgc caacaaggcg aggctttcgg cttcggcttt
      781 cggcattttt tgcattttca aaccgaatgc cgcattttcc agcgcactca tatggggaaa
      841 cagcgcgtaa tcttgaaaca tcagcgaaat acggcgtttt tcgggcggca tacgggtaat
      901 gttttccccg ttcagctgaa tttcgccgcc gtccggccgg acgatgcccg caatcatatt
      961 cagcagggtg gattttccgc agcccgaccg ccccagtacg gcgagtattt tgccgcgccc
     1021 gacagtcagg cagatgttgt cggcaaccgt tttgccgccg aagcgtttgc agagtccgtt
     1081 cagttcaagc atggcgcatc ctataaacgt atgccgtcca gccactcgga cagcggatgg
     1141 gcggcatcaa tcagaccgta acggcaaaac gcgtccagcg taaccagttg cgcgtcgtgc
     1201 atcatgtttc ccgacaacat ggtggcaacc aagctgccga tgtccatttt ttcaaaacac
     1261 gcaacctcgc cgtcctgatt ttcaggcagg aaggtttcgg gtaggacggc atcgaataca
     1321 tacaggattt cattgtgcac accccggctg acggggcgca ggctgtgcag ccgggatacc
     1381 ggacggataa acggaaacag cgttttatcc aaaccggctt cttcgctgct ttcgcggcac
     1441 acggcttcag acggcatttc gccgccggaa acaccgccgg cggcaatatt gtcgagtttg
     1501 tcgggatcga ctgctttgtg cggactgcgc ttgcctatcc agaaatgcca tcggccgtcc
     1561 gattcggtca gtccgttgag atggacggcg cggctgagca gtccgaacgg acggaaagcg
     1621 gcgcgttcga gcgtgaacaa ggggttgccg ccgccgtcgg tcaggtcgaa acactcgttg
     1681 cgccatccgt gaagcagccc cgccttgttc catgtgcggg cgaggtgctg caagcggccg
     1741 cccatatctg accagccgtc cgcattcagg aaaatgccgt ctgaagactc cgagcagcct
     1801 gcctcccagt cttttttgac gcgctccgcc cattccggcg acagattgcc caaaggcaga
     1861 cggttcagat acagcgtttt ccagcaactt tctgcaccgt aacttgcttt tgcccactcg
     1921 aacagggcat caaggtcgtg tttgctgacg gattcggtaa aacggacggt cggcatagtc
     1981 ggctttcttg aagacaatgc ggaagattgt agccaaactg cctgaatttg caaccgcaaa
     2041 cagacggcgg gctgccgttc cgatattgcg gcaggaagga aaatccggca aaaaacggag
     2101 gcggcaggaa acaggcaaaa caaaacccct cggcattgaa ccgaggggtt tgatatttgg
     2161 ttgcgggagc aggattcgaa cctacgacct tcgggttatg agcccgacga gctaccatgc
     2221 tgctccatcc cgcgtcagaa gatgaaacta tacggcagat tttttatgtt gtcaaacatt
     2281 atttccgcga aaaatcataa aattttgccg tccggcggtt tatgcctgat ttgaaatatc
     2341 attttcttta caaaagttca tgttcgtgat ttaattttgg ttaacattga aacaggggtg
     2401 ctgcatgatg ttcaggcggc tgagaaatac cctttacacc cgatctggat aatacctgcg
     2461 tggggagttt tcacggattc tgcctttcag acggcatttt ttgatactcg ggccgtttga
     2521 aaaagcaaag tgctcctgtt tctttattct aaacgagaaa acaggagcat tttttatgac
     2581 tacgccaaaa aaaactgcca aaacttccgg caacgaagca cgcgagcttg ccgacttgag
     2641 cgaagacatc ggcatccgct tcaaatatcc gaactcggaa cgcgtgtatc tgcaaggcag
     2701 ccgcgacgac atccgcgtgc ctttgcgcga aatccgccag gacgacacct acacggcgca
     2761 aggcacggaa gccaatccgc cgattcccgt ctatgacacc agcggcgtgt acggcgatcc
     2821 ggcggcacac atcgatttaa aacaagggct gccccacatc cgcacggcgt ggctggacga
     2881 acgcggcgat accgaaatcc tgcccaaact ctccagcgaa tacggcatcg aacgcgcgca
     2941 cgacccgaaa accgcccatc tgcgtttcaa ccaaatcacc cgcccgcgcc gcgccaaagc
     3001 cggacgcaac gtaacccaat tacattatgc ccggcaaggc attatcacgc ccgaaatgga
     3061 gtttgtcgcc atacgcgaac gtttaaaatt agacgaattg tcccaaaaac cggaatacgc
     3121 caagctcttg aaacaacacg cagggcaaag tttcggcgcg aatatcccga cccatcccga
     3181 ccaaatcacg cctgaattcg tgcgccgaga aatcgccgcc ggacgcgcga ttattcccgc
     3241 caacatcaac caccccgaac tcgaaccgat gattatcggc cgcaacttcc gcgtcaaaat
     3301 caacggcaat ttgggcaact ccgccgtcac ctccagcctg accgaagaag tcgaaaaaat
     3361 ggtgtggtcg ctgcgttggg gcgcggacac gattatggat ttgtccaccg gcgcgcacat
     3421 ccatgaaacg cgcgaatgga ttatccgcaa cgcgcccgtc cccatcggca ccgtgccgat
     3481 ttaccaagcg ttggaaaaaa ccggcggcat cgccgaagat ttgacttggg atttgttccg
     3541 cgacaccctc atcgaacagg cggaacaagg cgtggactat ttcaccatac acgcgggcgt
     3601 gttgctgcgt tatgtaccga tgaccgccaa ccgcctcacc ggcatcgtct cgcgcggcgg
     3661 ttcgattatg gcgaaatggt gtttggcaca tcatcgggaa aacttcctct acacgcattt
     3721 cgacgaaatc tgcgaaatta tgaaagcgta tgacgtatcg ttcagcctcg gcgacggcct
     3781 gcgccccggc tgcattgccg atgccaacga cgaatcccaa ttcgccgagc tgcacacctt
     3841 gggcgaattg acctccaaag cgtggaaaca cgacgtacag gtgatgatcg aaggccccgg
     3901 ccatgtaccg ctgcaacgcg tcaaagaaaa tatgaccgaa gagttgcaac actgttttga
     3961 agcaccgttt tacaccctcg gcccgctcgt taccgacatc gcccccggct acgaccacat
     4021 cacctcgggc ataggcgctg ccaatatcgg ctggtacggc acggcgatgc tctgttacgt
     4081 tacccccaaa gaacatttgg gactgcccga caaagaagac gtgcgtaccg gcatcatcac
     4141 ctacaaactc gccgcccacg ccgccgacct cgccaaaggc tggccgggcg cacagttgcg
     4201 cgacaacgcc ctgagcaagg cgcgtttcga gttccgctgg cgcgaccagt tccgcctaag
     4261 cctcgacccc gaacgcgccg aaagcttcca cgacgaaacc ctgcccgccg aaggcgcgaa
     4321 aatcgcccac ttctgttcga tgtgcggccc caaattctgc tcgatgaaaa tcacgcagga
     4381 agtgcgcgac tacgccgaca agcaaaaagc ccaacggcag ggcatggagg aaaaagcggt
     4441 cgagttcgtc aaaaaaggcg cgaagattta cagttgaaac gtcaagcaaa aaatgccgtc
     4501 tgaaacccgg aaaaaaggct tcagacggca ttctttcgct tgtgaaaata tagtggatta
     4561 acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg aaccgactca
     4621 cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta
     4681 ctggtttttg ttaatccact ataattttta aaatttttat attcacatag atggggatga
     4741 tgggatttag ggttctgatt ttgtttttga gagaatgaag gaatttgaga ttgtgggcga
     4801 ttatcgggaa aaatagaatc tttccgccgt cattcccgcg caggcgggaa tctagacatt
     4861 caatgctaag gcaatttatc gggaatgact gaaactcaaa aaactagatt cccactttcg
     4921 tgggaatgac ggcagagcgg a
//
LOCUS       DAWFJW010000126         4936 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFJW010000126 DAWFJW010000000
VERSION     DAWFJW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.126"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(650..1186)
                     /locus_tag="WGR19_001824"
     CDS             complement(650..1186)
                     /locus_tag="WGR19_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0799300.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVASEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1220..1576)
                     /gene="folB"
                     /locus_tag="WGR19_001825"
     CDS             complement(1220..1576)
                     /gene="folB"
                     /locus_tag="WGR19_001825"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0799301.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1632..2234
                     /gene="plsY"
                     /locus_tag="WGR19_001826"
     CDS             1632..2234
                     /gene="plsY"
                     /locus_tag="WGR19_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0799302.1"
                     /translation="MFNISAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIWATLLIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            2311..3156
                     /locus_tag="WGR19_001827"
     CDS             2311..3156
                     /locus_tag="WGR19_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0799303.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSKAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLKNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3402..4376
                     /locus_tag="WGR19_001828"
     CDS             3402..4376
                     /locus_tag="WGR19_001828"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0799304.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVDYLNRLHSK"
     gene            complement(4534..4752)
                     /locus_tag="WGR19_001829"
     CDS             complement(4534..4752)
                     /locus_tag="WGR19_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0799305.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1287 a         1452 c         1014 g         1183 t
ORIGIN      
        1 aagtattcat atattctatc tttaattatt tcattatttt cttcattttc ttcatctaaa
       61 aatatacatc tttttaatag ttccccatta aattcataaa catcgtaata agctttaaaa
      121 atagatatca tatcatttcc ttatcttcca taaatcggct tcgcttgttt aaatactgcc
      181 tgcgccaatt accgttctca aattcaaatt gggcttttag gtctataaca aagaaaaagc
      241 cttggcagga taaacctacc aaggcttttt attaaaaaaa acgctctatt actgtgtcta
      301 aaaatgcaaa aaaagtaacc atcataaccc ttttgccaaa atcaatatca tcatccgatg
      361 aatgaattga cggtatataa gaaagaatta ataagctcat aaaccggtaa gaaaaatcag
      421 taacaaagat attcattatt aactattctt aaaaatgatc acgttcgttt gggtaattat
      481 cagcccatac cataccctac tcctattact aaagcacgaa tgactttaaa gggcgcaccc
      541 gacggcaagc cgaacccttg cctccccgaa gccgaaatca gaaaaaccat accccatccg
      601 ccaaacaaaa tgcccgccaa tctactccgg cgggcagttc aacaccctgt caatccttca
      661 tcagccagta ttgcaaaccg attaatgtct tgccgtcttt aatttcatcg tttgccaatg
      721 cctgacggac ttcttctttc gacatcaata cggtttccgt aatctcgtct tcgtcattgg
      781 caagcgtact gcccaaacgc acgccttccg cctcgaacag atacattttt tcgttgcaaa
      841 aaccgaccgc cgtataaaaa ctgtaaagca ggcgtacgct gtcggcggta taaggcgttt
      901 cttcagccag ttctcgtaaa gcgcatgctg ccatatcctc gctcgccaca tccagcttgc
      961 ccgcaggaag ttccaatgtc gcctgatttg ccgcataacg ccactgccgc accaaaacca
     1021 ctttcccctc gtccgtaacc gccaacacgc atgccgcacc cggatggcgg atgacgatac
     1081 gctgcccttc attgccgttg ggcaacctga ccttatccct gctgatgctg acgaaaccgc
     1141 cctcgtaaat ggtttcgccg cctaatttta cttccctcaa atccacttca tatctccttt
     1201 ccgtttccta ttctgccgtt caatcttcac gcttgccgcg ctcgatttcc acgccaacct
     1261 cgcgcacgcc ttccaaaata cccggcttga cgattttcac gcgcacccac accgcgccga
     1321 aatatcccaa aaccaaatcg gcaatatatt ccgccaacgc ttccaaaagc aggaaatcct
     1381 gttctttcag atgtcggcgc agcgtttcgc atacctcggc ataatgcacc gtattggcaa
     1441 tatcgtcgtc cgaacccgct ttctcgggaa caccgatgtc caaatcgaca atcagggtct
     1501 gcaaccgttc gcgctcccag ccgtacacgc cgataagcgt atccgccttc ataccgtgca
     1561 aaaagatttt atccataatc cgccccgttt tttgctaaaa taaccgcacc attctaagta
     1621 aagtaccgca aatgttcaac atatcggctg ttgctgtttc ctatctgatc ggctcacttt
     1681 ctttcgccgt catcgtgtcc aaatattacg gcatggacga cccgcgcacc tacggatcgg
     1741 gcaatccggg ggcaaccaat gttttacgca gcggcaaaaa aaaggcggcc gcgctgacgc
     1801 tcttgggcga tgccgccaaa ggtttagttg ccgttttgct tgcacgcgtg cttcaagaac
     1861 cgctcggttt atccgacagc gcaatcgcgg ccgtcgcact cgccgcgctg gtcgggcata
     1921 tgtggccggt gtttttcgga tttaaaggcg gcaaaggcgt ggcaacggca ttgggcgtgc
     1981 ttctggcact ctctcctgca actgccttgg tctgcgcgtt gatttggctt gtgatggcat
     2041 tcggcttcaa agtatcctcc cttgccgcat taaccgccac aatcgccgcc ccccttgccg
     2101 cactgttttt tatgccgcat acttcttgga tttgggcgac actccttatc gccctgctgg
     2161 tgttgttccg ccacaaaagt aatatcgtca agctgctcga aggcagagaa agcaaaatcg
     2221 gcggcagccg ctgatttccc atccatccct gtttatgccg tctgaaccac gcttcagacg
     2281 gcatccgaat atcccgccgt tttaccgact atgctcagtt acagacacgc attccacgcc
     2341 ggcaaccacg ccgatatgct caaacacttt accttgtttt tagtgctgca atatttcaac
     2401 cgcaaagaca agccctactg gtacatcgac acgcacggcg gcgcgggtgt gtacaatctc
     2461 gaaggcagca aagcacagaa agtcggtgaa taccgccaag gcatcgccct gctccgacag
     2521 gcgcaaaacc tgcctgccga actctctgac tttgccgcgc acatacaaaa aatcctgccc
     2581 tcgcccaaac tttactgcgg ttctccgtgg ctggcgcaat cgctgacccg tgtcggcgac
     2641 aaattgcgcc tgtttgagct gcaccccacc gattttgtcc atctgaaaaa caatatgggc
     2701 gaagcaggat tgggcaaacg tggacaagta ttgcgcgaag acggttacaa agggctgatt
     2761 tccctgctgc cgcccccgcc gcgccgcgcc accgtcctca tcgacccgcc ctatgaggaa
     2821 aaacaggact accggcgtgt aacggaaacg ctgaaagccg ctttaaaacg gtttgagtcc
     2881 ggctgttatc tcatttggta tccctgcctc agccgcgaag aaagccgcaa gctgcccgaa
     2941 gaattgaaaa aactcctgcc cgacaactac cttcacgccg aacttcacgt ccacgcgccg
     3001 aaagccgacg gtttcggtat gcacggcagc ggaatgttcg tcatcaatcc gccctatctg
     3061 ttggcggaac aattggcggc aaacctcccc gccctgacgc ggctgctggc gcaagacgaa
     3121 ggcgcgcgct accttttgga cagcaaaatc cgctgacccc gatgccgtct gaaacccttc
     3181 agacaacatc tcccgtcaaa taaacaaaac cggacgatat accggcagtc ggcactttta
     3241 agaataaata tcccctcatc cgcctgccgc gttccgactt gccccatgcc gtttttttac
     3301 actacaatcc cctagactat cctgcaaaac gcaccaaagg aatacgccac aataacctgc
     3361 cgcctttcag acggcacaaa cctttccgaa acgagcaaac tatggacaca ctgacccgat
     3421 tcctccccga acacctgcaa caaaaccagc tgcccgaagc actcggcggc gttttactgt
     3481 ccgttgtttc cgcctgcacc gaaatcaacg ccaaagtccg cctcggcgca ctggccggcg
     3541 tattgggtat ggcgggcacg ggcaatattc agggcgaaga ccaaaaaaaa ctggatgtta
     3601 tcgccaacaa catcatgatt gacacactca aagccaaccc tgccgttgcc ggtttggcaa
     3661 gcgaggaaga agacactttc gtaagtgccg gtgaaaacgg acgctatctc gtcctattcg
     3721 accctttaga cggatcgtcc aatattgatg tcaacatttc cgtcggtacg attttctcca
     3781 tccttgaaaa acccgaaggc gcattggcaa ccgaatcatt ccttcaaacg ggtagacagc
     3841 agcttgccgc aggctatgtc ctctacgggc ctcaaaccca gctcgtattc acattcggac
     3901 acggcgtata tatgttcacg ctcaatgccg aaaacgaatt tgtgctgaca aaagaaaaac
     3961 cgaaagtacc cgaaagtacc aaagaatttg ccatcaatat gtccaaccgc cgccactggc
     4021 tgccccccgt ccaacaatac atcgacgagc tcttggcggg cgaaaccggc acgcgcggca
     4081 aaaactacaa tatgcgctgg gtagccagca tggttgccga aatccaccgc atcctgatgc
     4141 gcggcggcgt gttcatgtat ccgcaagaca aacgcgaccc cgccaagccc ggcaaactgc
     4201 gcctgatgta cgaagccaac cctatgagcc tgatactcga acaggcgggc gcatcggcaa
     4261 gcaacgcata tcaagccatg ctcgacatac agcccgaaaa cctgcaccaa cgcgtcgccg
     4321 tcttcatggg cagcagcgaa gaagtggatt acctcaaccg cctgcattca aaataaaagg
     4381 cagcctgacc gataaagaaa ggcggcagcg cgcatccgac gctgccgccg atatttcgtt
     4441 tatagcctta aaaagataga aaaccacctt tccgaacggc aaaaatgccg tctgaaaagt
     4501 tttcagacgg catttcaaaa actcaaaccc gaattatttt cccgctgctt tttgcaggat
     4561 ggcgtacaac tcgtctttca gtttcaattt ggcttttttc agctcatcga tggtttccgc
     4621 accgctggta accggattgt tgaccagacc agtaatttta tcgtccagct cgttgtgttc
     4681 gtcgaacaga cgggcgaagc gggaattttc ctgtttcaat ttggaaatca aatcacggta
     4741 ttctgggaac atatcgcttt cctctcttgg tttacggtta aaaagtcaaa ttgaatcttt
     4801 acattaatta taacaaagat aatattttta tccagtgttt tatcggatac ggcaaaattt
     4861 tcttttaaca ccgccgtccg gttgataaaa acgttgacaa tatcacgctt tacgcataaa
     4921 ttttcagcct ttccta
//
LOCUS       DAWFJW010000127         4916 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFJW010000127 DAWFJW010000000
VERSION     DAWFJW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.127"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..104)
                     /locus_tag="WGR19_001830"
     CDS             complement(<1..104)
                     /locus_tag="WGR19_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0799306.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTVRSAADILG"
     gene            276..497
                     /locus_tag="WGR19_001831"
                     /pseudo
     CDS             276..497
                     /locus_tag="WGR19_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(479..787)
                     /locus_tag="WGR19_001832"
     CDS             complement(479..787)
                     /locus_tag="WGR19_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799307.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK
                     "
     gene            complement(846..1077)
                     /locus_tag="WGR19_001833"
                     /pseudo
     CDS             complement(846..1077)
                     /locus_tag="WGR19_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1013..1240
                     /locus_tag="WGR19_001834"
     CDS             1013..1240
                     /locus_tag="WGR19_001834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799308.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            1402..2457
                     /gene="aroG"
                     /locus_tag="WGR19_001835"
     CDS             1402..2457
                     /gene="aroG"
                     /locus_tag="WGR19_001835"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0799309.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            2525..3013
                     /locus_tag="WGR19_001836"
     CDS             2525..3013
                     /locus_tag="WGR19_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0799310.1"
                     /translation="MLKLTLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLHVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(3363..3839)
                     /locus_tag="WGR19_001837"
     CDS             complement(3363..3839)
                     /locus_tag="WGR19_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0799311.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(3851..4279)
                     /locus_tag="WGR19_001838"
     CDS             complement(3851..4279)
                     /locus_tag="WGR19_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0799312.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQNYSNTFSHRWYRVFNEIPVLLMIGALYLVVFKPF"
     gene            4391..4636
                     /locus_tag="WGR19_001839"
     CDS             4391..4636
                     /locus_tag="WGR19_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799313.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGLDFQTAMREEKGNKEIASIIH
                     GGLPVLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
BASE COUNT         1302 a         1134 c         1248 g         1232 t
ORIGIN      
        1 atacccaaaa tatcggcggc agaacggacg gtaacttcca gcacaaaaaa acggagcagt
       61 tctttttgta cttttttctt taatttgcag tgcgttatct tcatattccg agggtaacat
      121 atctgctaat ctagtacagc ccctaacaaa aaccagtacg gcgttgcctc gccttagctc
      181 aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta
      241 ctatctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccgctatatt ttagataatg
      301 cgcgatttca ccgtatgggt gtcttacggg aaatggcgga aaaattggga cataaggtat
      361 tgcctcttgc accttattca cctgagctca acccgattga gaaagtgtgg gcgaatatta
      421 agcggtatct gcgaaccgtt ttgtctgatt acgcccgatt tgacgatgca ctactgtcct
      481 actttgattt taattgacta tagaacgttg cggttacgcg gaagccgtac tcgttggatt
      541 tggagcggcc cattttggtt ttgtcgccgt ccaaaacaat ctcacggggt ttgcagattg
      601 ttttgtgacg gtagtatgga tcaaactcga gaccgacgct gtcggtcaac tgtttgccta
      661 cattcagacc gataccgaca ctccaacctt tggcgctttt gctgacatcg cgggaagcac
      721 acatctgggt cgtcatcact ttggttttgc cgcgcaaatc tgcatatgca tccgcccaag
      781 gggtcaagga tcatccgtcc cccaaatctt ggcggatttc gccatagact ttcaaaacaa
      841 ggttttcatg cttggtaacg gtgtttttcc ttatcgccga tgatggcttt gcccttgccg
      901 ttagactcag gaatatcggc taccgtaacg gcggacacgg ctgcaagtga gagtgcaagc
      961 agggtttttt catgttttct tcctataatg aggatgaata aatggagaaa gtgtgggaaa
     1021 tacccgcatt ccaattaaat cttttttcaa gcaattagtt ctttttgttt tcaacatttt
     1081 ccttgcgtat gaaattttgc cggatgtgca ggcggaatcg gcagcggggt gttctgtacc
     1141 ggattgtcgt ggaaatggga aaacggatgt tccgtgcggt tttgtccgaa tgaatggtgg
     1201 gtattgtttt tatcaatctg tttctttttc tttggaataa aattcctaaa atattaaaaa
     1261 tatgaaattt aaaatctatg aaaaaagata tatcagttat tttgaaataa aatagctttg
     1321 tagtaatatg ttgcacttgt ttgtgcaagg taaacgatgt aacctaagcc gcgtataaaa
     1381 acccatcagg aaagatgcaa gatgacacac cattacccca cagacgatat taagattaaa
     1441 gaagttaaag agttgttgcc gccgattgcc catctttacg agctgccgat ttccaaagag
     1501 gcttcgggct tggttcaccg cacccgtcag gaaatttccg atttggttca cggcagggac
     1561 aagcggctgt tggttattat cgggccgtgt tcgattcacg atccgaaagc ggcgttggaa
     1621 tatgcggagc gtttgttgaa actccgcaag cagtatgaaa acgagctttt gattgtgatg
     1681 cgcgtttatt tcgagaagcc gaggacgacg gtgggttgga aaggtttgat taacgacccg
     1741 catttggacg gtacgttcga tatcaatttc ggtttgcgtc aggcgcgcag cctgttgttg
     1801 tcgctgaaca atatgggtat gcctgcctct accgagtttt tggatatgat tacgccgcaa
     1861 tattatgcgg acttgatttc ttggggggca atcggtgcgc ggacgaccga aagccaagtt
     1921 caccgcgaat tggcaagcgg gctgtcctgc cccgtcggct ttaaaaacgg tacggatggc
     1981 aatttgaaga ttgccatcga cgcaatcggt gcggcgagcc attcgcatca tttcttgtct
     2041 gtaaccaagg ccggacattc cgccattgtc cataccggcg gcaatcccga ctgtcatgtc
     2101 attttgcgcg gcggaaaaga gccgaattat gatgcgggac acgtcagcga ggcggcggaa
     2161 caactgcgtg cggcaggggt aaccgacaag ctgatgatag attgcagcca cgccaacagc
     2221 cgcaaggatt acacccggca gatggaagtg gcacaagata ttgcagccca attggaacag
     2281 gacggcggca atatcatggg cgtgatggtg gaaagccatt tggtcgaagg caggcaggac
     2341 aagccggaag tgtacggcaa aagcattacc gatgcgtgta tcggttgggg cgcgactgaa
     2401 gaactgttgg cattgttggc aggtgcaaac aaaaaacgta tggcgcgcgc cggttgagat
     2461 ttttgacacg gaatgtcata aaatgccgtc tgaagcgttc agacggcatt tttgtggagg
     2521 aaatatgctc aaattaaccc taattgcggc gtgtgcggaa aacctgtgca tcggggcggg
     2581 caatgcgatg ccttggcaca tccccgaaga tttcgcattt ttcaaagcct ataccttggg
     2641 caaacccgtc attatggggc ggaaaacgtg ggaatccctg cacgtcaaac ccctgcccgg
     2701 acggaggaac atcgtcatca gccggcaggc ggattattgc gcggcaggcg cggaaacggc
     2761 ggcaagtttg gaggcggcat tggcattgtg cgcaggcgcg gaagaagccg tcattatggg
     2821 cggcgcgcag atatacggac aagcgatgcc attggcgacc gatttgcgga taaccgaagt
     2881 ggatttgtct gtggaaggag atgcattttt ccccgcaata gaccggacgc attggaaaga
     2941 agcagagcgg acggaacgcc gtgtcagcag caaaggcacg agctatgctt ttgtgcatta
     3001 tttgagatat tgaaatataa actctctata aaatcccccg caaatgatgg gctgaaatag
     3061 aaaatattgt tattcccccg aagatgggaa tccgggattt taaagttagg gtaatttatc
     3121 cgaaataaca acaatcttcc atcgtcattc ccgcaaaagc gggaatccgg aaacgaaaag
     3181 ctaaagcaat ttatcggaaa aaaccgaagt ttaaagaacc ggattcccgc ctgcgcggga
     3241 atgacgagat tttaggttat ggggatttat tgggaataat ggaacaaaga aagcagaaat
     3301 aaggatatag aggctgtctt tggatttgcg atggttgtcg gagaatgccg tctgaagccg
     3361 tttcagacgg catttttcca gcttgagaac ggatgcctgc tcaaataagc attggtaaac
     3421 ataccgtcgg cagtgatttc ccgtcccagc cagtccggac ggtcaaaatc ggcattctcg
     3481 tcgggcaact cgatttccgc gacgaccagg ggcgcgtttt cgccaaggaa aacatcgatt
     3541 tcaaacaggc tgccgcccca tctgaccgga taacgccatt tttccatttt aaacgggcac
     3601 atcgtttcca tcatcttttc cgcatctgca agcgggattt cgtattcaaa ctcactgcgg
     3661 ctgagttccg aaatatagcc tttcagcgtc agccacgcct gttttccggc aatgcggaca
     3721 cggacggtgc gttctttttc aacagataga taaccctgcc tcaacagcag cggctcatcg
     3781 gcgtattgcc gccagttgtc gtttccaatc aaaaaacggc gttcgatttc tatcggcata
     3841 agatgctccg tcaaaacggt ttgaacacaa ccaggtaaag cgcacctatc atcagcagta
     3901 cagggatttc gttaaatacg cgataccagc ggtgtgagaa agtattgctg taattctgaa
     3961 aacgacgcag cagcacgccg caatacaact ggtaagccaa aagcatcaag cccaaacaca
     4021 gtttgacgtg tacccagccg ctgccccacc agccggcggc aaacggtatc gccgcgccga
     4081 acacgaccgc gccgaagccc aacggcgaca taaaacggta cagccgcacc gccatatccg
     4141 acaggcgcac atactcggga ttgccgcgcg gcgcatcaat catcgccata ttgacgaaaa
     4201 tcctcggcag gtaaaacagc cctgcaaacc acgaaatgac aaaaaacaag tgaaacagct
     4261 tgaaccaaga aaacatcatc gcccacaccc tgccgaaaag cggtattgta caggcaaacc
     4321 gcttgggaaa cgtgataaaa tcaggcggat aaacaaatcg aataaatcct taccgcaaaa
     4381 cggaggcaaa atgctcaaat ccatcgaatt caattcccac atccgcaacc gccttgcagc
     4441 atatctgaaa ggcaggggtt tggattttca gacggcgatg cgggaggaaa aaggcaacaa
     4501 agaaattgcc tcaattattc atggcggcct gcccgtactg gttcgcaaac tgtattccga
     4561 acaaaaaatg cagaaatttt tttgggaaaa gcgggatttg attgccgact acatcagccg
     4621 ccggatgcag ggataggcgg ctgaaatctg ttttcaggca agtgaaaaga caatatggca
     4681 gattgaaatt acgcttatcg tcattcccgc ccgcgcggga atccgacttg tttggtttcg
     4741 gttatttttc gtttcgtaac ttttgagccg tcattcccgc gcaggcggta atccggcttg
     4801 ttcggtttcg gttctttttc tcgtttcggg tgatttctaa accgtcattc ccgcgcaggc
     4861 gggaatctag gtctttaaac ttcggttttt tccgataaat ttttgccgca ttaaaa
//
LOCUS       DAWFJW010000128         4901 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFJW010000128 DAWFJW010000000
VERSION     DAWFJW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.128"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..1305
                     /locus_tag="WGR19_001840"
     CDS             <1..1305
                     /locus_tag="WGR19_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0799314.1"
                     /translation="LKNLIQKAAAAQKLWAAKTALERADVLWRWYFQIKENKEALARL
                     MTMEQGKSLTEARGEIDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTA
                     AITPWNFPFAMIARKAAPALAVGCAMIVKPASLTPLSAYALASLAYEAGIPQDLLPVV
                     SGSASEIGHEFATNPIIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDD
                     ADLDKAVEGALASKFRNSGQTCVCTNRVYAQSAIYDEFCRKLSEKAAALKLGNGLEDG
                     VNQGPLIEEKAVEKVEQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGITAQMAVA
                     REETFGPLCPVFRFETEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGIN
                     TGLISNEAAPFGGVKRSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1414..2202
                     /locus_tag="WGR19_001841"
     CDS             1414..2202
                     /locus_tag="WGR19_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0799315.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2570..4126
                     /locus_tag="WGR19_001842"
     CDS             2570..4126
                     /locus_tag="WGR19_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0799316.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4143..4889
                     /gene="surE"
                     /locus_tag="WGR19_001843"
     CDS             4143..4889
                     /gene="surE"
                     /locus_tag="WGR19_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0799317.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
BASE COUNT         1135 a         1242 c         1423 g         1101 t
ORIGIN      
        1 ctgaaaaacc tgattcaaaa agcagctgca gcacaaaaat tatgggcggc aaaaactgcg
       61 ttggaacgcg ccgatgtgtt gtggcgttgg tattttcaga ttaaagaaaa caaagaagca
      121 ttagcgcgcc tgatgacgat ggagcagggc aaaagcctga ccgaggcgcg tggcgaaatc
      181 gattatgcgg cttcgtttgt gcgctggttt gccgaagagg cgcggcggat tgacggcgat
      241 gtgctgacga gtgtgaaagc gtcgcaaaaa ctggtcgtgt tgaaacagcc cgtcggcgtt
      301 accgctgcga ttacgccgtg gaactttccg ttcgcgatga ttgcgcgcaa ggccgcgcct
      361 gctttggcgg tgggttgcgc gatgatcgtc aaacccgcat cgctcacgcc tttgagtgcg
      421 tatgccttgg cttcgttggc ttacgaagcg ggcataccgc aggatttgtt gcctgttgtc
      481 agcggcagtg cttcggaaat cggccatgaa tttgctacga acccgattat ccgcaaaatc
      541 agcttcaccg gctcgaccga agtcggcgca aaaatttttg ccgacagcgc ggcggacatt
      601 aaaaaactca gtttggagct gggcggcaac gcgccgttta tcgtgtttga cgatgccgat
      661 ttggacaaag ccgtcgaagg cgcgctcgcc agcaagttcc gtaacagcgg tcagacctgc
      721 gtctgcacca accgcgttta cgctcaatcc gccatttacg acgaattttg ccgcaaattg
      781 agtgaaaaag cagccgcgct caaattgggc aacggcttgg aggatggtgt gaaccaaggg
      841 ccgctgattg aggaaaaagc ggtggagaaa gtcgagcagc acatcgccga cgcgcttgct
      901 aaaggtgcaa gctgcctgac cggcggcaaa cgcagcgcgt tgggcggaac gttttttgag
      961 ccgactgtct taagcggcat tacggcacaa atggcggtgg cacgcgaaga aaccttcggg
     1021 ccgttgtgtc cggtattccg ttttgaaacc gaagtcgagg tcatcgaggc tgcgaacaat
     1081 acggaatacg gtttggcggc ctaccttttc accgccgaca ccgcccgcca atggcgcgtc
     1141 ggcgaagcct tggaatacgg catggtcggc atcaatacgg gcttaatcag taatgaagcg
     1201 gcaccgttcg gcggcgtgaa acgcagcggt ttgggacgtg aaggcagcaa atacggtgcg
     1261 gacgaatatc tagaattgaa atatttgtgt atggatgtcg ggtgacggat gccgtctgaa
     1321 cggcagggtt tcagacggcg ccgcatttta agcagtctct atctgttgta caatgcgccc
     1381 tgtttttacg gttatttttg atttgaacaa gatatgatgg aaaacggaaa aacagtcccg
     1441 agatggcgtt ttgccttgaa aagtgcgggc tggcatctct taatcagcct gtcggttgca
     1501 gggctggcgg cattgctggt ttttaaggtt tggtatcctt atccttatgc cgagctgacg
     1561 ggagggctgt cgctttatca gctggtggtg gctgtcgata ttgtatgtgg tccgctgctg
     1621 actttaattt tggcaagccc gaagaaaaag acaaaggcac gcatggtcga tttttccatg
     1681 gtcggcatca tccagctggc ggctttggtg tacggtctgc acagcgtttc tttggcgcgc
     1741 cctgtgatag aggcgtttga aaaagacagg attaatgttg tgaccgccgc cgaagtggcg
     1801 gttgaagatt tgcacaaagc gccggaaggg ctgcaaagcc tgtcgtggtt cggcatccgc
     1861 cgcattgcat tgaaagaacc tgaggatgcg gatgagaaga acaagacgct ggatttgtcc
     1921 ctgaaaggta tcgagccgag tatgcgtccc gaccagtggc tgccgtattc cgacaaggaa
     1981 gcagaagaaa tccgcaaaca tctgaaaccg ctgaaagtct tggcggatgc gagaaaaacg
     2041 acggttgcgg acattctgaa acaggcaggt ctcgccgaag gggaggagct gtattacctg
     2101 ccgtttacca gcagcaggca gaaagagtgg atagtcatta ccgataaaga gggcaacacc
     2161 aaaggctacg cgccgataga cggcttcatc atcacccctt aagcgttggg actccgtccg
     2221 cactcgaaca tccgttcttc gcggcggtag aatcagactg tatttgagag gggcgcaacc
     2281 cctcaatcag ggcaacacat aggggaaggc tttatgtgcc gtccgtgtgt tgaaacattt
     2341 caaaataagc tcatgccgtc cgaacatcct ttcagacggt atggcgtttt gccattgccc
     2401 cgataagctg ttaaactatt taaattattt cgaccgaagg tacacgcaca tgcaagaccc
     2461 cgagcagagc agcaagcccg cccgccgttt tttgtgcgta ccgtctgttc aggcagcttg
     2521 agcgcttcag gctgcctttt cgcgtttgaa cttgaggaat acgaaaatca tggatttcag
     2581 ttggttggca gaaccgcata cctggatagg ttttgccacg cttttggtgt tggaagtcgt
     2641 attggggata gacaatcttg tctttgtggc gattttggca aacaaggtcc agcccgcacg
     2701 gcgcgaccgc gcacggatta tcgggctggg gctggcagtc gtcatccgca tcattatgct
     2761 tgcttttatg gcgcacatca tcacgctgac cgagccgctg ttccaaatcg gcggcctcgc
     2821 cgtttccggc aaggacatga ttatgctcgc gggcggtatt ttcctgcttt acaaagccac
     2881 caccgaactg catgaacgcc tcgaagggca caaccgtttt accgttgccg acaaccaaaa
     2941 aaaacacgcg ccgttttggg gcgtggtcgc gcaaatcctg atactggatg ccgtgttttc
     3001 catcgattcg gtcattactg cggtggcgat ggtcgatcat atcgtcgtgg cgatgggtgc
     3061 ggtcgtcgtc gcgatggctg taatgatttc tgccagcaaa ctcttgaccg aatttgtcga
     3121 cagacaccct accgtcgtga tgctctgcct tggttttttg ttgatgatcg gtttcagcct
     3181 gattgccgaa gccttccatt tccacattcc caaaggctac ctctacgccg ccatcggctt
     3241 ctcgatttta atcgaactgt ttaaccagat ttcccagcgc aacagccgca aaaacgacta
     3301 catcggcagc tcgtggcgca agcgcaccgc cgaaaacgtt ttgggtatga tgggcatacg
     3361 cgaaagcgtg cttgccgatg cgggcggcga atccggggac gacgcgcatt ttgaagaaaa
     3421 cgaaaaatcg atgatacgca gcgtgctgac gcttgccgaa cgcccgatta tgggggtgat
     3481 gatcccacgc cgcgacatcg aacggctgga catttcccaa agccgcgaag aacagtgtgc
     3541 ccaactgcaa aatacgcctt acagccgcct gctcgttgtc ggtaaggcgg gcgtggacga
     3601 acctttgggc tacatcaaca aaaaagacct gctgtcccaa ctgctggaaa caggcggtct
     3661 cgacattcag acggcattgc gccagccact cgtcctgccc gacagcacca ccgcgctggg
     3721 cgcaatcgaa ctcttccgcc aaagcagcgc ggattatgct ttggtggtgg acgagttcgg
     3781 cgcggtattg ggcatggtaa ccatgaaaga cctgctcgaa accatcgcag gcgagttccc
     3841 cgaagaattt gagcgcgaag aagaaccagc cgttcagggg aatcccgatg aaagcctgac
     3901 ggtggaaggc gcgttggaat atgtggagct ggcatcccaa ctcaatctgc cgcagcagga
     3961 ggaagatgcc gatttccata cggttgccgg gctgattatg gaagaattgc aaaccatccc
     4021 cgatgtcggc gattttgccg atttccacgg ctggcggttt gaagtggtcg aaaaagaagg
     4081 gcagcgcatc gagcgggtca aaatcaccaa attgcccgaa gaataagcat tcaggataga
     4141 aaatgaacgt tttgatttcc aacgacgacg gctacctctc cgaaggtatt gccgttttgg
     4201 cgcgcgttac ggcggaattt gccaacgtca gggtggttgc gcccgaacgc gacaggagcg
     4261 gggtcagcaa ttcgctgacg ctggaacgcc ctttgcagtt gaaacaggcg caaaacgggt
     4321 tctactatgt caacggcacg ccgaccgact gtatccacat cgggcagtct gtattttcgg
     4381 attttcaggc cgattttgtc ttttcgggca tcaaccgggg cgcgaatatg ggggacgaca
     4441 cgctttattc ggggacggtt gcggcggcaa ccgaagccta ccttatgggc atacccgctg
     4501 tggcgttttc cttaaacgac gcttccggac gctattgggc gaccgcagaa caggcactgt
     4561 ggacattgtt ggcgcatttt ttcaaaaacc ccccgcagtc ccctattttg tggaacatca
     4621 atatccccgc cgttgcgccg gaagatgtgc ggggcattaa aatcgcccgt ttgggcagga
     4681 ggcatcacgg tcagaacgtc atccccgcgc gcaatccgcg cggcgaacag atttattgga
     4741 tagggccggt cggcgaagtt tccgatcggg aagagggaac ggatttcggt gaatgcggcg
     4801 caggtttcat taccgtaacg ccgttgcaaa tcgacctgac cgcctatccg gacatggcgg
     4861 aaacggcggc gttctggcat acggactgac cgtttcatca a
//
LOCUS       DAWFJW010000129         4876 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFJW010000129 DAWFJW010000000
VERSION     DAWFJW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.129"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..1486)
                     /gene="rng"
                     /locus_tag="WGR19_001844"
     CDS             complement(<1..1486)
                     /gene="rng"
                     /locus_tag="WGR19_001844"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0799318.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIV"
     gene            1700..1957
                     /gene="grxC"
                     /locus_tag="WGR19_001845"
     CDS             1700..1957
                     /gene="grxC"
                     /locus_tag="WGR19_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0799319.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            1978..2421
                     /gene="secB"
                     /locus_tag="WGR19_001846"
     CDS             1978..2421
                     /gene="secB"
                     /locus_tag="WGR19_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0799320.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            2507..4549
                     /gene="recG"
                     /locus_tag="WGR19_001847"
     CDS             2507..4549
                     /gene="recG"
                     /locus_tag="WGR19_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0799321.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQVREIAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1071 a         1341 c         1390 g         1074 t
ORIGIN      
        1 gcactatatc gtattgttcc tgtgtgtaag cggtttcgac cgccaaagaa atcggtttgc
       61 cgatgaaatc tatcagcatt gccaaggatt gcgattcttc gtccaaaaac aaatcgatga
      121 cgttgggggc ggcgaggatg cggaaacttt cggcatcgta acggcgcgct tcgcggacga
      181 tttcgcgctg gatttcgtag cacacggttt gcggcgtttt cagacggcct ctgccttggc
      241 aggaagggca gggttcgcag aggacttggt ttaagttttc gcgcgagcgt ttgcgcgtca
      301 gctcgaccag ccccaggctg gtaaaaccgt gcagggtaac gcgggtacgg tcgaaggcga
      361 gggctttggc aagctcctgc aacacggctt cgcggtggct ttcctgcgcc atatcgatga
      421 agtcgatgat gatgatgccg ccgaggttgc gcagcctcaa ttcgcgggcg atggtgtggc
      481 aggcttcgag gttggtgcgg aagatggttt cgtcgaagtt gcgtgcgccg acgaagccgc
      541 cggtgttcac gtctatcgtg gtcatggctt cggtggattc gataatcagg tagctgccga
      601 agttgaggtt gacgcgcggt tgcagggcgc ggctgatttc ctgttcgacg ttgtgggttt
      661 caaacagcgg gcgttcgcct ttgaacaatt ctatcctgcc caatgcgccg tggacgtatt
      721 gttcggcaaa acgcgtcatc cgtccgtggt ttacggtgga atcgacgagg attttctgcg
      781 tgtcgcagcc gaccatatcg cgcaatacgc gcaaactcaa gggcaaatcc tgataaagca
      841 gggtttccgg cggccggatt ttcgcctgtt cttggatgtg ttcccacact ttggtcaggt
      901 agtcgatgtc ggactggagc tgttcgtcgg tggcgttttc ggcgttggtg cggatgatgt
      961 agccccggca ggcattttcc ggcaggagct tgtcgaggcg ttcgcgcagg ctgctgcgtt
     1021 cggcatcgtc ttcgatgcgt tgggacacgc cgatgtggtc ttcttgcgga agatggacga
     1081 ggaaacgccc cgccagcgag atttgggtgg aaagccgcgc gcctttggtg ttgatcgggt
     1141 ctttgatgac ctgcaccaaa acagactgcc cttcaaacag catatgttcg atgcgctggg
     1201 tttcttcggg gttgcggcgt tgttcgagga catcgacgat gtgtaaaaac gccgcgcgtt
     1261 ccaagccgat gtcgataaac gcgctctgca tcccgggcag cacgcggcgc accacgccca
     1321 gatagatatt gccgaccagg ctgtgttcgc tgttgcgctc gatgtgcagc tcgcagatat
     1381 tgttttcctc caacaccgct acgcgcgttt cctgcggcgt gatgttgacc aatatcgttt
     1441 cgggcgggcg cgcgatgtct ttggggatgg ggagtcctga caacatggtt tttcctgaaa
     1501 atgtaataaa aatatttttg cacccgttcc gccgcgcagc gcgggtgcaa aaatattttt
     1561 cacaatgtct atcatacttt aaaaaagaaa ctttgacaca ttccgcacat gccgtttgaa
     1621 cgtgcatctg ttccgcactt gccaaacagg ggaaatgccc ctatattgat tatcattgca
     1681 aaactttcgg aaacccaata tgcagaccgt taccatgtac acaggtccgt tttgccccta
     1741 ctgcgcgatg gcgaaaaggc tgctgcacgc ggcaggtgtc ggacatatcg acgaaatccg
     1801 tgtcgatgca agccccgaag cctttgccga aatgcagcag ctttcgggac agcgcagcgt
     1861 gccgcagatt ttcatcggcg aaacgcacgt cggcggattt accgacctct accgcctcca
     1921 gcaggaaggc gggctggacg gactgctgaa cccttaaccc ccaactagga aaacaaaatg
     1981 agcgaagaac tgcaacccgt attcagcatc gagcgactgt atgtcaaaga cttgtctttg
     2041 gaagtgccgc acgcgccgca aatctttttg gaacagggcg agcctgaagt ggatatgcgc
     2101 gtttccaccg gcagccaaaa gctggaagac ggctactaca acgtggacgt taccgtaacc
     2161 gttaccgcca agctggataa cgagcgcacg atgtttttga acgaagtaac ccaaagcggc
     2221 atcttccgtt tggaaaacat ccccgaagaa gatgtgcagc tgctgctggg cgtggcgtgt
     2281 ccgaacatcc tcttccctta cgcgcgcgaa gccgtttccg gtacggtaac gcgcgccggc
     2341 ttcccgcccg tcctgctcgc gccgattaat tttgaggcga tttaccaaca acagcaggaa
     2401 gccgaagccg ccggggcttg attcctgcct gtcgatgccg tctgaatccg tttcagacgg
     2461 catttttgtt ttcggataaa ataggcgcgt ccaaaggaat ctgcctatga tgtcgcccga
     2521 aacccgaaaa cagctcaaaa tcaccgatgt ttccgccaag aagctcgaca aactcaacct
     2581 ccataccgcg tgggatttgg tgttgcacct gccgctgcgt tacgaggacg agacgcacat
     2641 tatgccgatt aaggacgcgc cgattggcgt gccgtgtcag gtcgaggggg aggttatcca
     2701 tcaggaagta acgttcaaac cgcgcaagca gttgattgtc caaatcgccg acggttccgg
     2761 cagcgtcctt tttctgcgct tcatccactt ttacgccagc catcagaaac agacggcggt
     2821 cggtaagcgc atccgcgccg tgggcgaaat caaacacggc ttttacggcg acgagatgat
     2881 tcatcccaaa atccgcgatg ccgagggcgg cggtttggcg gaaagcctca cgccggttta
     2941 cccgaccgta aacggtttga accagcccac tttgcgccgt attattcaga cggcgttgga
     3001 cgttacgccg ctgcacgaca cgctgcccga tgccctgctg tgccgtctga agctgccaca
     3061 cctcgccgaa agcctgcgtc ttttgcattc gccgccgccg agtttcacga ttcatcagct
     3121 ttcagacggc acgctgcctg catggcaacg gctcaaattc gatgaacttt tggcgcaaca
     3181 gctttcgatg cgcttggcgc gacagaagcg tatcggcggc acggcggcgg cattgggcgg
     3241 cgacggcaca ttgacccaag ccctgcgcca agccctgccg tttgccctga ccgatgcgca
     3301 agaaaaagtt gtttccgaaa tctgccgcga tatggcgcaa acctacccga tgcaccgcct
     3361 gctgcagggc gatgtcggca gcggcaaaac cattgtcgcc gccttgtccg cgctgaccgc
     3421 catcgaatcc ggtgcgcagg tggctgtaat ggcgcccact gaaatccttg ccgaacaaca
     3481 ttttattaaa tttaaacaat ggctcgaacc tttgggcatt gaagttgtcc gcctttttgg
     3541 cagtttgcgt aaaaaagcca aagacgaagc caaagccaaa ctcgccgacg gcagcgtcaa
     3601 aatcgccgtc ggcacgcacg ccctgttttc agacggcgtg gcgtttcaca atttgggctt
     3661 gaccattgtg gacgaacagc accgtttcgg cgttgcccaa cgcctcgccc tcaaaaacaa
     3721 agggcgcgaa gtccatcagc tgatgatgtc cgccacgccc atcccgcgca cgcttgcgat
     3781 gagttttttc gccgatttgg acgtgtccgt catcgacgaa ttgccgcccg ggcgcacacc
     3841 gattaaaacg cgcctcgtca acaacgtccg ccgcgccgaa gtcgaaggct tcgtcctcgg
     3901 cacttgccga aaagggcggc aggcgtattg ggtctgccca ttgattgaag aaagcgaaac
     3961 cctgcaactg caaaccgccg ccgaaaccct cgcccggctt cagacggcat tgcccgaact
     4021 caatatcgga ctggtacacg ggcgtatgaa ggccgccgaa aaggccgaag tgatggcgcg
     4081 gttttcttcg ggggggctga acgtcttggt cgccaccacc gtcatcgaag tcggcgtaga
     4141 tgtgcccaat gccgccctga tggtcatcga acacgccgag cgcatgggct tggcgcagct
     4201 gcaccaatta cgcggtcggg tcgggcgcgg cgcggcagaa agcgtgtgcg tcctcctgtt
     4261 tgccgaaccc ttgggcgaac tcgccaaagc gcggttgaaa gtgatttacg aacacaccga
     4321 cggctttgaa atcgcccgcc aagacctcaa tatccgcggc cccggcgaat ttctcggcgc
     4381 gcgccaaagc ggcgtgccta tgctgcgctt cgccaagctt gaagaagact tacacctttt
     4441 ggaacaagtg cgcgaaatcg ccccgatgct gattgagcaa aaccccgaaa tcgtcgaagc
     4501 gcatttggca aggtggcttt ccggcaggga agggtatttg ggcgtgtgag caaaatgccg
     4561 tctgaaatat tagaaaaaaa gtgtaacgga aatctgattt tgcattattt ttgacacatt
     4621 ttcatgattt caaatcccgt tattcccaag tggacggaaa cccgataatg ggaagccgca
     4681 gagacttatc gggaaaacgg caagccctcc gccgtcattc ccgcgcaggc gggaatctag
     4741 aaataaaaaa ccacggtgtt atcggcaatg actgaaaccg gagaaatgac ggaatcgaag
     4801 cgtgtggaaa tgtgtcggaa attgctgaag ccggataaac tagattcccg cttttgcggg
     4861 aatgacggga ttttag
//
LOCUS       DAWFJW010000130         4868 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFJW010000130 DAWFJW010000000
VERSION     DAWFJW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.130"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..414)
                     /locus_tag="WGR19_001848"
     CDS             complement(<1..414)
                     /locus_tag="WGR19_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0799322.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGINKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VPNTQTATLFPIIREQVKPDSIVYTDCYRSYDVLDV"
     gene            complement(557..1912)
                     /locus_tag="WGR19_001849"
     CDS             complement(557..1912)
                     /locus_tag="WGR19_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0799323.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1927..2199)
                     /locus_tag="WGR19_001850"
     CDS             complement(1927..2199)
                     /locus_tag="WGR19_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0799324.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(2325..3062)
                     /locus_tag="WGR19_001851"
     CDS             complement(2325..3062)
                     /locus_tag="WGR19_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0799325.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(3343..4242)
                     /gene="prmB"
                     /locus_tag="WGR19_001852"
     CDS             complement(3343..4242)
                     /gene="prmB"
                     /locus_tag="WGR19_001852"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0799326.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            4387..>4868
                     /locus_tag="WGR19_001853"
     CDS             4387..>4868
                     /locus_tag="WGR19_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799327.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     "
BASE COUNT         1371 a         1251 c         1000 g         1246 t
ORIGIN      
        1 cacatctaat acatcatagc tacgataaca atccgtataa acaatactgt caggtttcac
       61 ttgttcacgg ataataggaa ataaagtagc ggtttgagta ttcggtactg taaccgtata
      121 aaccttacca tttcgcttca aaagaccgaa tacggcgact ttaccggcag caccgcgacc
      181 gcgtttgcct ttgcgttgtc cgccaaaata actttcatct gcttctactt cgccatcaaa
      241 catttccaaa tgcggactgt tttgataaat aagtaatcgt aaacgatgaa aataataggc
      301 tgcggtattt ttattaatgc ctactaactc tgctgctgtt cttgcagtta cacctgcgac
      361 aaatagctca atgagtttat tttgtttata ccggcttaga cgactttttc tcatagggat
      421 aattctaact taatttgaat ttccctagtt atctaggaca gccccatatt cttttctaaa
      481 gacattttac ccgatgccta taaacaaaaa tgccgtctga agcctttcag acggcatttc
      541 cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg agccgggatt ctgccgcctc
      601 ggttaacaaa cacttcgcac gaaccttctt taaccggcat cacaggcgcg tagcccaaca
      661 ggccgccgaa ctcgacgctg tcgccgacgg ttttaccggt taccggaata atgcgcacgg
      721 cagtggtttt gctgttgatc atgccgatgg cggcttcgtc ggcgatgatg ccggaaatgg
      781 tatgagcagg cgtatcgccg ggaacggcaa tcatatccaa accaacggag cagacagcgg
      841 tcatcgcttc aagtttgtcc aacgtcagca caccggcttc ggcagcggca atcatgccct
      901 cgtcttctga aacggggata aacgcaccgc tcaaaccgcc gaccgcgctg gaagccatca
      961 tgccgccctt tttcacggca tcgttcagca atgccaaagc cgccgtcgtg ccgtgtgtac
     1021 cgcagacgct caaacccatt tcttcaagaa tgcgcgccac cgagtcgccg acggcagggg
     1081 tcggtgccaa cgacaagtcg agaataccaa acgggatatt cagcattttt gaggcttcgc
     1141 ggccgatgag ttcacccacg cgggtaattt tgaaagcagt tttcttcact acttccgcaa
     1201 cttcggtcaa tgtcgttgca tctgaatttt ccaacgcggc tttgaccacg cccggccccg
     1261 atacgccgac attaatcaca gcatccgctt cgcctgagcc gtgaaacgcg cccgccataa
     1321 acggattgtc ttccaccgca ttgcagaaca cgacgatttt cgcgcagccg aaaccttcgg
     1381 gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag cttgaccgca tccatattga
     1441 taccggcgcg cgtactgccg acattgatgg agctgcacac gatgtcggtg gtcttcatcg
     1501 cttcgggaat ggaacggatt aacacctcgt ctgaaggcga catgcctttt tgcaccaacg
     1561 cggaaaagcc gccgataaag gacacgccga tggctttggc agccttatcc aaagtttgcg
     1621 ccacgctgac ataggaatcg gcatgggtag ccgccgcgat ttgggcaatc ggcgtaacgg
     1681 aaatgcgctg attcacaatc ggcacgccgt atttggcaga cagatatttt gccgtcgcca
     1741 ccaagtcttt gccgaccgtg gtaattttgt tgtaaatgtt ttggttcaac acattgatat
     1801 cgctgctgat gcagtcgtgc agatcaatgc cgatggtaat ggtgcggaca tcaaaattct
     1861 ggtcggcaac cattttgacg gtttctaaaa tttcgccgga ttggatactc atcacattcc
     1921 tccgactcaa atgcggtgca tcgcttggaa gatttcttca ttttgcatac ggatatcaag
     1981 cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc tcctgccgag atttggggca
     2041 tttcgaggta tccaccaaga taatcatggt aaaaaaatca tccatcagct gttggctgat
     2101 attgaggatg ttgattcggt tttccgccaa aattttggaa acatcgtaca cgatgccgac
     2161 gcggtcttta ccgatgacgg tgatgactga attgttcaca ggcttactcc ttgcagatat
     2221 ccgttaaagt ccgaaattat accaccgttg gattttgaag aaatattgtc aacaatatat
     2281 acatacaaaa tgccgtctga aactatttca gacagcatca agattcaggg ttcgattaaa
     2341 taaccatcct tatcccactg ggttttcctg accaacttgt catcctgata aacagcttca
     2401 ctctttttag aaccatcttc ataccactct aagactactc cattacgttt atgatgatgg
     2461 atagacagtt ccgagagtaa tcgaccgctt tcatcccatg tcagaatctt ggccggctca
     2521 tcattgacca taaccatttc cgttttgata ttgccatcag cataccattg tttccatacg
     2581 ccgtttgcct tattttgttt aaactggatt tcgctttcct tgctgccatt acggtaatag
     2641 cggtaccccg taccttcact caaaccattt ttataaggca taaccgcaga ttttttaccg
     2701 ttcggatacc agttgaccca ctcaccgtcc ggcttaccct tgctgaagcc ccccgccatt
     2761 tttttctgac cattaaaatg ccacaaaatc aacataccgt tttgcagggt aggcacaaaa
     2821 gatttgattt gcgttgaagc aacgatataa ggttcggaat atttcttcat cgacggataa
     2881 taaaaatcct gcgcgtacgc aataccctcc gccacactat attgcctgat ataagcggca
     2941 gaagacatcg tcgccgtcag tttcccgttc tgattaaaat aaacagaata ggtctgtgcc
     3001 gataaagaag ccgaaaaacc caacaggaca attgaaaata caatccgaga taattttttc
     3061 attgcaatag cgatataaaa acaaggctgt gttttagtaa tctgttgatt tcaattattt
     3121 gcaagggaaa agacaattat tttccggtta ggaataaacc tattctactg aatatattga
     3181 agccaagtac gcctatcaac actatattaa aacactgcca aaaacaatta acttataaac
     3241 aatatggtaa ggatttctct gccaagcatc aaacccgaga caacgtatcg taaaaatgcc
     3301 gtctgaaaac aaatcgtctt cagacggcat ttccccttca actcactctt cacccaataa
     3361 ctgctcgcgc gtcaagagga aaacaaaacc gtcgcccccg ctggtttcca accaagtaaa
     3421 aggcaactcc ggatacgctg cttccaatac atccctgtta tgcccgattt ccaccagcaa
     3481 tacacctttg ggattcagaa actttgccgc attcagaaga atctgcctgg tggcatccaa
     3541 cccgtccgcc ccgctgccca atgccaattc cggttcgtgc aaatactctt caggcaataa
     3601 ctcaaccgat tccgcatcca cataaggcgg attggaaaca atcaaatcat aagtgccttc
     3661 caatccttca aacaaatccg tatgaataag ccggatgcgt tcttccaaac cataatcttc
     3721 gacattaatc cctgccactt ccaaagcatc caagctcaca tcaaccgcat caatttgggc
     3781 atcaggataa tgatgcgcca tctgaatggc aaggcaaccg cttccggtgc aaagatccaa
     3841 agcattatgc accaactcat cgtattctat ccaaggacga agtccgtcac ccaacaattc
     3901 ataaataaaa gaacgaggta tgattacgcg ctcatccaca tagaaatcaa actctccctg
     3961 ccatgcctgg tgtgtcaaat aagcggctgg aatgtgttcg acagcacgac gctcaataac
     4021 cgtcagcact tcctcttttt cagcttccaa gagttttgca tcaagatatg gggcaagcat
     4081 atccaaaggc aaattcaaag tatgcagaat caaataagct gcttcatcat gcgcattatc
     4141 cgttccatga ccaaaaaaga gccctgcctc attaaaacgg ctgactgcaa aacgtaaaac
     4201 atcgcggata gtcgtcaatt cttgtgctgc ctgattaaac ataatatgaa ccattctgcg
     4261 tatagatact tttaattata acagaaacaa caagcaaacc ttttcatatc gccaaataac
     4321 cacccaatct acccatacaa ctacataaat gcccgcgcga aaaccatcgc ccgaacggaa
     4381 acgacaatgg ccgacggtat gggcaacctg attggctggg aaaaaacggg gcttgttgcc
     4441 ggtaagcagt ggataaccgc aaaagacgac aaggtgtccg atgtctgcaa tgccaacggc
     4501 gagatgggcg taatcgggct ttacgagcct ttctcacacg gcgcattgac gatacccggt
     4561 catccgaact gccgatgcga ggttgtttcc gtatcgggtg gcgaattggg ggaatttgcc
     4621 gaaaaaaagg agcttcgtaa agcggctatg cagtatgcgc gggataactt tatcggcaaa
     4681 agctatgtca atgaaaacag cgggcatgaa ctgaaggtaa cttggcaagg tgtgaaacac
     4741 gctgcgtcaa aggcaaatca ggcggaatta tccatcatga caaaacttga tgacttattg
     4801 cgctacgcaa aatatgaggg ttcttattcg gataggaaag gtcatcctaa tattattgca
     4861 gcacataa
//
LOCUS       DAWFJW010000131         4759 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFJW010000131 DAWFJW010000000
VERSION     DAWFJW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.131"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..132
                     /locus_tag="WGR19_001854"
     CDS             <1..132
                     /locus_tag="WGR19_001854"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0799328.1"
                     /translation="DAYLHEIAKHFDCTAATICYALKQMGITRKKRPPLTKNKTRPK"
     gene            183..629
                     /locus_tag="WGR19_001855"
     CDS             183..629
                     /locus_tag="WGR19_001855"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799329.1"
                     /translation="MDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGN
                     RLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHK
                     VLPLAPYLPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
     gene            complement(793..2196)
                     /locus_tag="WGR19_001856"
     CDS             complement(793..2196)
                     /locus_tag="WGR19_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0799330.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDIVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSQQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2265..3692)
                     /locus_tag="WGR19_001857"
     CDS             complement(2265..3692)
                     /locus_tag="WGR19_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0799331.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAALKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3928..4281
                     /locus_tag="WGR19_001858"
     CDS             3928..4281
                     /locus_tag="WGR19_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799332.1"
                     /translation="MFTTNDLRHFLEGLVILFAIGYWDTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4569..>4759
                     /locus_tag="WGR19_001859"
     CDS             4569..>4759
                     /locus_tag="WGR19_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799333.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSV"
BASE COUNT         1149 a         1241 c         1161 g         1208 t
ORIGIN      
        1 gatgcctatc tgcatgaaat cgccaaacat tttgattgta cggcagccac catttgctat
       61 gcactcaaac agatggggat aacgcgcaaa aaaagaccac cacttacaaa gaacaagacc
      121 cggccaaagt aacgcattat ttgacacagc cggccgaatt ttccgactac caacgtgttt
      181 atttggatga aacaggattt gaccgctacc tgttccgtcc ctatgcccgc agcctgaaag
      241 ggcaaatagt gaaagcgcag ataagtggaa aaagatacca acgcttatct ttggtgtccg
      301 cacaagtcgg caaccggctg attgctccga tggtttatca aaatacgatg accggagtct
      361 tttttgaagc gtggtttcag caatgcctac tgcccgcatt gactcaaaaa tcggtgatta
      421 ttttagataa tgcgcgattt caccgtatgg gtgtcttacg ggaaatggcg gaaaaattgg
      481 gacataaggt attgcctctt gcaccttatt tacctgagct caacccgatt gagaaagtgt
      541 gggcgaatat taagcggtat ctgagaaccg ttttgtctga ttacgcccga tttgacgatg
      601 cactactgtc ctattttgat tttaattgaa tatacatttg aattaattcg cacttaattt
      661 aaatgtgttt ttaactgtgc tttatttaaa ggcaatgaga atgtgaaagt atcggatcaa
      721 tcccaaagca gcctgcactt tcgaaacggg gtgcaggctg ctttgggaat ttcataaccg
      781 tttcagcctg ctttattccg caaataccgt ttccaaccct aacccgctct ctttcaccaa
      841 gcgcaaataa gccagcatga atttataccg tgcttgagcc agtttctgtt ctgcttgggc
      901 gacttcctgc cgcgcccgta ttacttccag ccggttgcgg atgccgtatt gttggccggt
      961 ttcggtcgat ttcagtttca aacggctgct ttccaaaacc cgttcttgcg ccatgatttg
     1021 gtaacgcgcc gcaccgcttt cggtataagc ctggcgtacg gcgagtttga tgtgccgctc
     1081 ggttgcggtc agctgtgctt cggcggcccc gtattgcgct tcggcttcat ggattttgcc
     1141 cgacaattct ccgccggtat aaagcggcaa attcaactgt acgccgacgc tcatcccttt
     1201 gccccgatag tggtagtcat tattctgcgc agatgaagtg tagaggttat tctgatagcc
     1261 gacatgggca gaaacggtgg gatagcggct gttctgtgct gcccgaagcg cctgtccgct
     1321 gctttgcagg gcaagctgct gcatccggta ttcatgattg ttggataagg caatgcgctg
     1381 ccattcatcc agactgtaac gttccagctt gggcagatag cgtgccaaca ggttggcggt
     1441 atctatggcc tcgatttgtt ggctgtccag gccggtgtag tcgttcaact ggttttcata
     1501 ggtttgtttc tcagccaata cggcgatttc ttgggccagg gcattgtcgt aaccggcttt
     1561 ggcttcgtga atatccagcg cggtggcagc acctttattg aataaagcct gcgcctgcct
     1621 tacctgctgg gcataagcct ctttttccgc cgcatgggcg gcaacgatgt ctcggctgag
     1681 taaaacgttg aaataacttt cggcaacttt caacagcaat tcttcgcgtg ccgcatcgaa
     1741 acgctgttct gcagcctgcg tatcgaacct gctttggcgg tattgtgcaa atttggcagc
     1801 gtcaaataag gtttgtccca cctgcacgct ccatccctgt gtttcgcggg tggaagaaat
     1861 cgatggcggc tggcgctggt agctggcatt ggcggataca tggggaagga atgcggcctt
     1921 ggcttgttgt tgccgtgcgc gcactgcatc acgctggtaa tgggacgctt gaaaatcagc
     1981 cgaatgttgc tgcgccgccc gccatgcttc aggcagcgta aaagccgaaa cggatgggga
     2041 aagggatagt ggcaaggtaa aaagtgaaac gggtaggata tatttggaaa aataggattt
     2101 catagccgaa aatagttcat gttgcaaata gggcgtcagt gtcaggcaaa cggaaatacc
     2161 gtaatcttgc attatcatta gattgagcaa tgtcatccgg gcaatggttt caggcagtct
     2221 gcatgtccga accggcggat aacaaatgcc cagtatggat ccgcctatcg ctccctaaag
     2281 ctttcgtcca atttggtttg cagcgggctt aacagataat ccagcacccg ccgtttaccc
     2341 gttttaatct ccgccgtgac attcatgccc gccgtcagat tcactgcttt gccgtcaata
     2401 ttcaaggtat gtttgtccag cgacaccacc gccgtataaa ccaagcccaa ctgttcgtgg
     2461 cttaccgcat catggctgac acttttcacc ttgcccgtca gataaccgta gcgcgtatag
     2521 ggaaaactct caatcttcac caccgcatcc tgtccctgtt ccacaaaacc gatgtctttg
     2581 ttcaatacca aaacttccac gtccattttg tcgtcatcgg gcgcaaccac catcattttt
     2641 tgggcagcct gcaccacacc gcccaccgta taggtggcca attcctgcac cgtgccgtcc
     2701 gcaggcgact gtattgtcat cagctgctgc cgctgctttg ccttatccgt ttggccgcgg
     2761 tattggtcaa tctgttcgtt tgcctggcgc agcgcatcca gcgtatcgcg tttcaggttc
     2821 tgcgtattca gcacccgact ctgctccgcc tgtgcaatgg ccgcctgaat ctgcctcatc
     2881 tgaccgcgcg tactttccaa atcgttccaa ttgctgaccg atttgctctg ctgctccaaa
     2941 aacgcatgtt ccgaaataaa attgtcggcc cgcaaacggc ggtagtctgc tgttttctgc
     3001 tgctcgatcg cccccaccga aaccagcttc tgctcctgcg ccttggccga ctgcaattcc
     3061 gcctgatggc cgcgcaaagc cgactgcaat tgcgcatcct gcgccgccca tgcctgatac
     3121 tggtgctgcg ccaacacctg cgccgattgc acatcggcat cggagagacc taaagaccgt
     3181 gcttgcgcca tatcgatatg cggcacggta cggctttcca atgccgccaa tactgcttca
     3241 taacgcagtt tggacaattg ggcagcctgc aaagcctgct ccgactgcac cacatcgctg
     3301 tctgttccca cagcctccag ttccgccagc gtttctccct gtttcacatg ctgcccgtcg
     3361 cgcacatgta ccgccttaac caccgccgtt tccagcggct ggatggtttt gctgcgcccg
     3421 cccgacaccg ttttgcccga agccgccgcc acaatatcga ttttgccgaa ccaggaccac
     3481 aacaaagcca aaagcgcaaa cgccataata aaacgcgccg cccatttcag agcggcagag
     3541 accggcgtat cggtcagttc caaatgcgcg ggcaaaaacg cctgttcttc cgccgtgcgt
     3601 ttgggcggtt ccaactggtc gcgcaccgcc caaacattgc gccatacagt aatgtagcga
     3661 gaaagaaagg atttcagggc ggagaaaaac ataacgggta taaccttggc aatatagaaa
     3721 caggaaacaa tataaatatg taaaggaatt ttaacggaaa gcgcggcagc tgttaaggga
     3781 aaggcgggaa tattgacaaa aaatacccaa gtcgttacaa atattcatta ttttactgcg
     3841 taacgcaacg ctgaagcgca ggctgctttt gagatgcggc aaggttcggc aaaaagcagc
     3901 ctgcacattt aaccacagga actacccatg tttaccacaa acgatttacg ccatttccta
     3961 gaaggtttgg tcatcctatt cgcaatcggc tattgggaca ccatgctgct gttgctttgg
     4021 tttctcgtcc gctttgccta taaaaagccc aaacggaacc ccggcaaaat cctttcaggc
     4081 agcctgaccg ccgccgctat cctgatgctg tttgtttgga ttattcccaa acaattcggc
     4141 ccgatcaaag aagaaataca ggcacaagaa gagtgggaca gaaaatacaa agaagccgaa
     4201 gccgtgttta acgaacaatg caaaacggcg ggggaaagat ttaccagacg gcggacaatg
     4261 tggaagggat tatgctgttg aaggtagtac ctgagcgtac cgtttcggca gatgcaaaaa
     4321 ccagagaccc gatgtgggac aatgcggctt tacagaccag cgaaggcgta aattttattg
     4381 ctcgtttcct aggatttttt agcgatgggg aataccgcta tgtggatgtc ctgcaaccca
     4441 accattccga tattattcgg tattcaggta aagattttcc gataaatcaa atacttaatc
     4501 atatacaccc cgcccgttat gcggtaacgt tcgaaaacaa tgtcgattcc aagctgcgca
     4561 ggcactgggt ggcaggtgcg accatacgga ttatcgaccg ccaaactgac gaagtgattg
     4621 ccaagaaaac catctatgtc tttgaaaaag gcttggacgg cacgggtggg gcgagaatgc
     4681 cgtggaagtt tgctatcttg tgcaataaag aaagacttac ttcttcagag ccgttatcgg
     4741 attttgttct tagcgtttt
//
LOCUS       DAWFJW010000132         4675 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFJW010000132 DAWFJW010000000
VERSION     DAWFJW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.132"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            82..1047
                     /locus_tag="WGR19_001860"
     CDS             82..1047
                     /locus_tag="WGR19_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0799334.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESSKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRGAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1065..1214
                     /locus_tag="WGR19_001861"
     CDS             1065..1214
                     /locus_tag="WGR19_001861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799335.1"
                     /translation="MPSETRMQTAYRVSDGIARYGGFKQGFPLRRFGLPCLNLLRLNA
                     FPFCL"
     gene            1211..2179
                     /locus_tag="WGR19_001862"
     CDS             1211..2179
                     /locus_tag="WGR19_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0799336.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGSGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIVGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2169..3143
                     /locus_tag="WGR19_001863"
     CDS             2169..3143
                     /locus_tag="WGR19_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0799337.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AINLGINYTRNTLWLLLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3188..3814
                     /locus_tag="WGR19_001864"
     CDS             3188..3814
                     /locus_tag="WGR19_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799338.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVSGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3899..4657
                     /locus_tag="WGR19_001865"
     CDS             3899..4657
                     /locus_tag="WGR19_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0799339.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKVALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT          960 a         1376 c         1283 g         1056 t
ORIGIN      
        1 ttatcattta tcctttctag agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacaccc tatgttgcgt ttgaccgcat tgaccctctg caccgtcctc
      121 gccttgggcg cgtgttcgcc gaaagattcc gactctgccc cacaagccaa agaacaggcg
      181 gtttccgccg cacaatccga aagcgcgtcc gttaccgtca aaaccgcgcg cggcgatgtt
      241 caaataccgc aaaaccccga acgtatcgcc gtttacgatt tgggtatgct cgacaccttg
      301 agcaaactgg gcgtgaaaac cggtttgtcc gtcgataaaa accgcctgcc gtatttagag
      361 gaatatttca aaacgacaaa acctgccggc actttgttcg agccggatta cgaaacgctc
      421 aatgcctaca agccgcagct catcatcatc ggcagccgcg cagccaaagc gtttgacaaa
      481 ttgaacgaaa tcgcgccgac catcgagatg accgccgata ccgccaacct gaaagaaagc
      541 tcaaaagagc gtatcgacgc gctggcgcaa atcttcggca aacaggcgga agccgacaag
      601 ctgaaagcgg aaatcgacgc atcttttgaa gccgcgaaaa ccgctgcgca aggcaaaggc
      661 aaaggtttgg tgattttggt caacggcggc aagatgtccg ccttcggccc gtcttcacga
      721 ctgggcggct ggctgcacaa agacatcggc gttccagccg ttgacgaagc cattaaagaa
      781 ggcagccacg gccagcctat cagctttgaa tacctgaaag agaaaaaccc tgattggctg
      841 tttgtccttg accgcggcgc ggccatcggc gaagagggtc aggcggcgaa agacgtgttg
      901 gataatccgc tggttgccga aacaaccgcc tggaaaaaag gacaggtggt ttacctcgtt
      961 cccgaaactt atttggcagc cggtggcgcg caagagctgc tgaatgcaag caaacaggtt
     1021 gcggatgcgt tcaacgcggc aaaataatga aacggcggca ttcgatgccg tctgaaacac
     1081 ggatgcaaac cgcctaccgt gtttcagacg gcattgcccg atacggaggc ttcaaacaag
     1141 gctttccgct ccgacggttc ggactgcctt gtttgaatct tctacgcctt aacgcttttc
     1201 ccttctgttt atgactgcca aacctttttc cctcaacctg actaacctcc tgctgctggc
     1261 ggtgttgttt gccgtcagcc tgtcggtggg cgttgccgat ttccgctggt cggatgtgtt
     1321 ttcgctgtcg gacagccagc aggttatgtt catcagccgc ctgccgcgca cgtttgcgat
     1381 tgtgttgacg ggcgcgtcga tggcggtggc ggggatgatt atgcagattc tgatgcgtaa
     1441 ccgttttgtc gagccttcta tggcgggctc gggtcagagt gcggctttgg gtttgcttct
     1501 gatgtccctg ctgctgcctg ccgcgccgct gccggtcaaa atgtcggttg ccgccgttgc
     1561 cgcgttaatc gggatgttgg tgtttatgat gcttatccgc cgcctgccgc cgacggcgca
     1621 actgatggtg cctttggtcg ggattatttt cggcggcgtg gttgaggcgg tggccacctt
     1681 tattgcgtat gaaaacgaaa tgctgcaaat gctgggcgtg tggcaacagg gcgatttttc
     1741 gagcgtgctg ctggggcggt acgagctgct ttggattacg ggcggtttgg cggtgtttgc
     1801 ctatctgatt gccgaccggc tgacgatttt ggggctgggc gaaacggtca gcgtgaattt
     1861 gggtttgaac cggacggcgg tgttgtggtc ggggctgatt attgtggctt tgattacgtc
     1921 gctggttatc gttacggtcg gcaatattcc gtttgtcggg ctggtcgtgc cgaacatcat
     1981 cagccgcctg ataggcgaca ggctgcgcca aagcctgcct gcggtggctt tgttgggcgc
     2041 gtctttggtg ttgctgtgcg acattgtcgg acgcgtgatt gtgtttccgt ttgaaattcc
     2101 ggtctctacg gtttttggtg tgttggggac ggcgttgttt ttgtggcttt tattaaggaa
     2161 acctgctcat gccgtctgaa aaaaatatcg gttttatggc aggaagcagc cgtccgttgt
     2221 gggtcgcctt tgcgctgttg ctggtttcct gcgtcctgtt tatgacgctc aacgtcaaag
     2281 gcgattggga ttttgtactg cacctgcggc tgaccaagct tgccgcgctg ctgatggtcg
     2341 cctatgcggt cggcgtgtcc acgcaactct tccaaacgct gaccaacaat ccgattctca
     2401 cgccctcgat tttgggtttc gattcgctgt atgtgttttt gcagaccttg ctggtgttta
     2461 cgttcggcgg cgtgggctat gcttccctgc cgttgacggg caaattcggc tttgaactgg
     2521 tcgttatgat gggcggctcg ctgctgttgt tctacacgct catcaaacag ggcggacgcg
     2581 atttgccgcg tatgatttta atcggcgtga ttttcgggat tttgttccgc agcctgtcat
     2641 cgctgctttc gcgcatgata gaccccgaag aatttacggc ggcgcaggcg aatatgtttg
     2701 ccggattcaa taccgtacac agcgagcttt tgggcatagg cgcgctgatt ctgctcgtca
     2761 gcgcggcggt cgtttggcgc gaacgctacc gcttggacgt acaccttttg gggcgcgacc
     2821 aagccatcaa tttgggcatc aactacacgc gcaatacctt atggctgctg ttgtggatcg
     2881 ccgcgttggt ggcgacggcg accgccgtgg tcggcccggt aagctttttc gggcttctcg
     2941 ccgcctcgct tgccaaccac ttttccccgt ccgtgcgcca ttccgtccgc ctgccgatga
     3001 cggtttgtgt cggcggcatc ctcctggtcg gcggacagac cgtattcgaa cacttcttgg
     3061 gcatgaaggc ggtattaagc gtggtggtcg aatttgcggg cggactcgtt ttcctctatc
     3121 tcgttttaaa acacaaaaaa tgacggatgc catctgaacg gcagcccgcc ccgaaaggac
     3181 aaaccatatg acgcaagaac gtttacccga atttttcgac cgcgccccga cgctgaccgt
     3241 acaagacccg cttgccgcat tcctcggcgc ggccgaaaac ggcatcctca cttaccgcta
     3301 cgccgatgcc gtgcgcctgt gcggacattc ctgcccgacc gtcgcgggcg cgtacctgat
     3361 ggttatcaaa ggtctgaaag ccctttacgg agaagagctg cccgaacgcg gcggcatcga
     3421 agccgccatg cagggagcgc gcgacgaagg cacggtcggc gtaaccgcgt ccgtcgtcca
     3481 acttctcacc ggcgcagccc ccgaaaccgg cttcggaggc atcggaatgc agggacgctt
     3541 cgcccgccgc aacctcttat cctttggtgt aggcgaaatc aacggcacac tgaccctgcg
     3601 ccgcaaagac aacggcaaaa ccgtcgccgt cggcctcaac gccgccctgc aacccttcgc
     3661 acccgaaatg cgcgacatca tgcccaaagc cgtcagcggc agcgcaagcg cagaagaact
     3721 cgaacgcttc ggacaactct ggcaggcgcg cgttaaagca tttttgaccg aatcggcgga
     3781 cgacccgcag ttcgtcatcg tccgcgaagt gtgaacgttc agacggcatt ccgaatttca
     3841 aatgccgtct gaacccccgc caaacataca acaaacctac gcccgacaag catccgccat
     3901 gattaccatc cgcaacgtca gctaccgcat cggcacacgc cccatcctcg acaacgtcag
     3961 cctcgacatc cccgaaggcg gcattaccgc cctcgtcggc cccaacggtg cgggcaaatc
     4021 caccctgttt tcctttatgg cgcggctgcg cccgcttgaa agcggcagca tcgcctaccg
     4081 aggcaaaaat cttgccgaca cccccaccgc cgaactcgcc cgcacgctct ccatcctcac
     4141 ccaagaaaac agcatcatga gccgcattac cgtgcgcgac ctgctcatgt tcggccgcta
     4201 tccctaccat caaggcagac cgactgccga atgccgccgt atcgttaacg gtgcaatcga
     4261 agaattccac ctgcaagacc tctccgaccg ctacctgacc gagctctccg gcggacaacg
     4321 ccaacgcgcc atgattgcga tggtgttctg ccaaagcacc gactacgtcc ttttggacga
     4381 accgctgaac aacctcgata tgtatcacgc ccgctcgctc atgcagattc tgcaaaagct
     4441 cacgcgcgaa cacaaacgca ccaccgtcgc cgtattgcac gacattaacc aggccgccgc
     4501 ttacgccgac cacgtcgtcg ccatgaaaaa cggcaaagtc gccctgaccg gcacgcccga
     4561 agaagtgttc accgtacaca acatcaaaga actgttcgac atggatgtgg acgtactcga
     4621 ctacgaaggc aaaaaactca tcgtccacca tatctgaagc tcaaaaagaa aatgc
//
LOCUS       DAWFJW010000133         4478 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFJW010000133 DAWFJW010000000
VERSION     DAWFJW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.133"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..880
                     /locus_tag="WGR19_001866"
     CDS             <1..880
                     /locus_tag="WGR19_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0799340.1"
                     /translation="RYLRDHRGRPAYPLLSMLKAVLLGQWHSLSDPELEHSLITRIDF
                     NLFCRFDELSIPDYSTLCRYRNWLAQDDTLSELLELINRQLTEKGLKIEKASAAVVDA
                     TIIQTAGSKQRQAIEVDEEGQVSSQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGY
                     IEKLHITPANAHECKHLSPLLEGLPKGTTVYADKGYDSKENRQHLKEHQLLDGIMRKA
                     CRNRPLTETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMC
                     LNLLKAANRLSAPAAA"
     gene            1252..2331
                     /gene="nalP"
                     /locus_tag="WGR19_001867"
                     /pseudo
     CDS             1252..2331
                     /gene="nalP"
                     /locus_tag="WGR19_001867"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
     gene            2642..4158
                     /locus_tag="WGR19_001868"
                     /pseudo
     CDS             2642..4158
                     /locus_tag="WGR19_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1187 a         1242 c         1218 g          831 t
ORIGIN      
        1 ccgttacctt agagaccacc gcggccgtcc cgcctatccc ctgctgtcca tgctcaaagc
       61 cgtcctgctc ggacaatggc acagcctctc cgatcccgaa ctcgaacaca gcctcatcac
      121 ccgcatcgat ttcaacctgt tttgccgttt cgacgaactg agcatccccg attacagcac
      181 cttatgccgc taccgcaatt ggctggcgca agacgacacc ctgtccgaat tgctggaact
      241 gattaaccgc caactgaccg aaaaaggttt aaaaatagag aaagcatccg ccgccgtcgt
      301 tgacgccacc attattcaga ccgccggcag caaacaacgt caggccatag aagttgacga
      361 agaaggacaa gtcagcagcc aaaccacacc gagtaaggac aaagatgccc gttggataaa
      421 gaaaaacggc ctctacaaac tcggttacaa acaacatacc cgtaccgatg cggaaggcta
      481 tatcgagaaa ctgcacatta cccccgccaa tgcccatgag tgcaaacacc tgtcgccgtt
      541 gttggaagga ctgcccaaag gtacgaccgt ctatgccgac aaaggctacg acagcaagga
      601 aaaccggcaa catctgaaag aacatcagtt gttagacggc attatgcgca aagcctgccg
      661 caaccgtccg ctgacggaaa cgcaaaccaa acgtaaccga tatttatcga agacccgtta
      721 tgtggtcgaa cagagcttcg gtacgctgca ccgtaaattc cgctatgcgc gggcagccta
      781 tttcggactg attaaagtga gtgcgcaaag ccacctgaag gcgatgtgtt tgaacctgtt
      841 gaaagcggcc aacaggctaa gtgcgcccgc tgccgcctaa aaggcagccc ggatgcctga
      901 ttatcaggta tccggggagg attaaggggg tatttgggta aaattaggcg gtatttgggg
      961 cgaaaacagc cgaaaacctg tgtttgggtt tcggctgtcg ggagggaagg gaattttgca
     1021 aaggtctctt tctgcctgct taggcggcgg cggcggcact tctgcgcccg acttcaatgc
     1081 aggcggcacc ggtatcggca gcaacagcag ggcaacaaca gcgaaatcag cagcagtatc
     1141 ttacgccggt atcaagaacg aaatgtgcaa agacagaagc atgctctgtg ccggtcggga
     1201 tgacgttgcg gttacagaca gggatgccaa aatcaatgcc cccccccgaa tctgcatacc
     1261 ggagacttta caaacccaaa tgacgcatac aagaatttga tcaacctcaa acctgcaatt
     1321 gaagcaggct atacaggacg cggggtagag gtaggtatcg tcgatacagg cgaatccgtc
     1381 ggcagcatat cctttcccga actgtatggc agaaaagaac acggctataa cgaaaattac
     1441 aaaaactata cggcgtatat gcggaaggaa gcgcctgaag acggaggcgg taaagacatt
     1501 gaagcttctt tcgacgatga ggccgttata gagactgaag caaagccgac ggatatccgc
     1561 cacgtaaaag aaatcggaca catcgatgtg gtctcccata ttattggcgg gcgttccgtg
     1621 gacggcagac ctgcaggcgg tattgcgccc gatgcgacgc tacacataat gaatacgcat
     1681 gatggaacca agaacgaaat aatgtctgca gccatccgca atgcatgggt caagctgggc
     1741 gaacgtggcg tgcgcatcgt caataacagt tttggaacaa catcgagggc aggcactgcc
     1801 gaccatttcc aaatagccaa ttcggaggag cagtaccgcc aagcgttgct cgcctattcc
     1861 ggcggtgata aaacagacga gggtatccgc ctgatgcaac agagcgatta cggcaacttg
     1921 tcctaccaca tccgtaataa aaacatgctt ttcatttttt cggcaagcaa tgacgcacaa
     1981 gctcagccca acacactgac cctattgcca ttttatgaaa aagatgctca aaaaggcatt
     2041 atcacagtcg caggcgtaga ccgcagtgga gaaaagttca atggctccaa ccattgcgga
     2101 attactgcca tgtggtgcct atcggcaccc tatgaagcaa gcgtccgttt cacccgtaca
     2161 aacccgattc aaattgccgg aacatccttt tccgcaccca tcgtaaccgg cacggcggct
     2221 ctgctgctgc agaaataccc gtggatgagc aacgacaacc tgcgtaccac gctgctgaca
     2281 acggctcagg acatcggtgc agtcggcgtg gacagcaagt tcggctgggg actgctggat
     2341 gcgggtaagg ccatgaacgg acccgcgtcc tttccgttcg gcgactttac cgccgatacg
     2401 aaaggtacat ccgatattgc ctactccttc cgtaacgaca tttcaggcac gggcggcctg
     2461 atcaaaaaag gcggcagcca actgcaactg cacggcaaca acacctatac gggcaaaacc
     2521 attatcgaag gcggttcgct ggtgttgtac ggcaacaaca aatcggatat gcgcgtcgaa
     2581 accaaaggtg cgctgattta taacggggcg gcatccggcg gtagcctgaa cagcgacggc
     2641 attgtctatc tggcagatac cgaccgatcc ggcgcaaacg aaaccgtgca catcaaaggc
     2701 gatctgcagc tgggcggcga aggtacgctg tacacacgtt tgggcaaact gctgaaagtg
     2761 gacggtacgg cgatgaccgg cggcaagctg tacatgtcgg cacgcggcaa aggggcaggc
     2821 tatctcaacc gtaccggaca acgtgttccc ttcctgagtg ccgccaaaat cgggcgggat
     2881 tattctttct tcacaaacat cgaaaccgac ggtggtctgc tggcttccct cgacagcgtc
     2941 gaaaaaacag cgggcagtga aggcgacacg ctgtcctatt atgtccgtcg cggcaatgcg
     3001 gcacggactg cttcggcagc ggcacattcc gcgcccgccg gtctgaaaca cgccgtagaa
     3061 cagggcggca gcaatctgga aaacctgatg gtcgaactgg atgcctccga atcatccgca
     3121 acacccgaga cggttgaaac tgcggccgcc gaccgcacag atatgccggg catccgcccc
     3181 tacggcgcaa ctttccgcgc agcggcagcc gtacagcatg cgaatgccgc cgacggtgta
     3241 cgcatcttca acagtctcgc cgctaccgtc tatgccgaca gtaccgccgc ccatgccgat
     3301 atgcagggac gccggctgaa agccgtatcg gacgggttgg accacaacgc tacgggtctg
     3361 cgcgtcatcg cgcaaaccca acaggacggt ggaacgtggg aacagggcgg tgttgaaggc
     3421 aaaatgcgcg gcagtaccca aaccgtcggc attgccgcga aaaccggcga aaatacgaca
     3481 gcagccgcca cactgggcat gggacgcagc acatggagcg aaaacagtgc aaatgcaaaa
     3541 accgacagca ttagtctgtt tgcaggcata cggcacgatg cgggcgatat cggctatctc
     3601 aaaggcctgt tctcctacgg acgctacaaa aacagcatca gccgcagcac cggtgcggac
     3661 gaacatgcgg aaggcagcgt caacggcacg ctgatgcagc tgggcgcact gggcggtgtc
     3721 aacgttccgt ttgccgcaac gggagatttg acggtcgaag gcggtctgcg ctacgacctg
     3781 ctcaaacagg atgcattcgc cgaaaaaggc agtgctttgg gctggagcgg caacagcatc
     3841 actgaaggca cactggtcgg actcgcgggt ctgaagctgt cgcaaccctt gagcgataaa
     3901 gccgtcctgt ttgcaacggc gggcgtggaa cgcgacctga acggacgcga ctacacggta
     3961 acgggcggct ttaccggcgc gactgcagca accggcaaga cgggggcacg caatatgccg
     4021 catacccgcc tggttgccgg tctgggcgcg gatgtcgaat tcggcaacgg ctggaacggc
     4081 ttggcacgtt acagctacgc cggttccaaa cagtacggca accacagcgg acgagtcggc
     4141 gtaggctacc ggttctgacg gacaggaagc agacagccgc aaagatcacg gtctttgcgg
     4201 ctgtttctta tgaaaagaaa accctattcc aattgcctgc ttctattgtt tcaagacttc
     4261 ttccaaagat tcggcattaa tcagatgtat agcggattaa caaaaatcag gacaaggcgg
     4321 cgaagccgcg gacagtacaa atagtacgga accgattcac tcggtgcttc agcaccttag
     4381 agaatcgttc tctttgagtt aaggcgaggc aacgccgtac tggtttttgt taatccgcta
     4441 tattccacca tctctaagat ttacagcgat acacaggt
//
LOCUS       DAWFJW010000134         4435 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFJW010000134 DAWFJW010000000
VERSION     DAWFJW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.134"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..638)
                     /locus_tag="WGR19_001869"
     CDS             complement(<1..638)
                     /locus_tag="WGR19_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0799341.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAG"
     gene            1912..4044
                     /locus_tag="WGR19_001870"
     CDS             1912..4044
                     /locus_tag="WGR19_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0799342.1"
                     /translation="MSIVSAPLPALSSLIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            4269..>4435
                     /locus_tag="WGR19_001871"
     CDS             4269..>4435
                     /locus_tag="WGR19_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0799343.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWHPIEQYLNRQKNR
                     YLRDHRGRPAYP"
BASE COUNT         1147 a          983 c          995 g         1310 t
ORIGIN      
        1 caacctgcgc caccagtaag taaaccaaaa gccccaacac ccaaagccgc accccattgg
       61 acgcccatta gcccacccaa aaaagaagct gatgagagac caacatcact ccaatttcct
      121 tctccatcca aggctttcca ccaagcactt cccatactca aagttcccat ggcaacagtt
      181 gccccataag taaacttacc caaattcgct gccataactt ttgattttga agcattctct
      241 aacgctgttg ccgcacgtct tgcatattgc tgcttgttat agttgctagc attcaatgct
      301 ttttgcaaat cactttgtac ttttcttgca acaaatgcct cctttgaatt accacgaaat
      361 ttactagcag ctcttccaat tccttctttt aagctttttt gtgcctcttc tgctgtgcgt
      421 tggaattctc ttgccttagc agacagacgc tcaccctccc ttaaaaattc tttttgatct
      481 atcttgtgcg aacgggtcaa atattcttcg tacattgagt taatcgtacc aatcgcccta
      541 accacatttt catcagaaaa tatggaaata atagcatccc tatacgcacc tagtgtaata
      601 ttgtttctat tattagttat agcattattc gaatacataa tagcacctcc aaatttagaa
      661 ctgaatacgt ttatattctc tgtgtaataa tatcaaataa ttaattaatg gataagtaag
      721 tccgaaaaaa taacaactaa aataagcaat atactgattc tctcctttat taattagata
      781 tattgaaaaa caaataaaaa ataaacagaa aacattcatg tgaaaaagcc acctcatatt
      841 cttatttata agtttaaaat aactaggcat tgtatattta aaaatcggta catccaacgg
      901 aaacaaacca cataaaaatt tttctttatt ctttattaaa gacatagttt tatttttcaa
      961 aattagtcca gataatatac taacaattac tattaaaaca ataagcacag cgtaattttt
     1021 tataaaattc attaaaataa taacgaaaat actaaataga ttacatacta taaattgaaa
     1081 tataaaatat tcagtattta atccattttc aaccgcgagt tttttaaaat atccttgctc
     1141 gaaaaaatta aaaaaatcca ttaatacttt cctctcaaga aaactaattt tagtttagtt
     1201 aagataaaat gcaatattac cttttctcca taaaagtatg gagcttatat acataaatta
     1261 tgtgcgaggt agcctgaaaa atatccaata caagatagta ggagatgttt cccttatcac
     1321 tattggccaa tacccaattg gcaggcactc tatttccaaa attttctcaa gaatagttaa
     1381 atactattaa ttagtttaaa aacaataaca tacgtttgta tttatccgtt ttggtctgcc
     1441 tgaaacagac tctatgtcca tacacggaca tgtagacagt ttccttgtta ttttggttgc
     1501 tcgtcattag tgatgatact ttactcctct cttaaaaaat cgtcaagaac tgttatcaat
     1561 ctttaaagtc ttttttaaac aaatatatat acgttcaggc tgtctgaaac acaaaaaaat
     1621 gatgttaccc gaccgtgaca gagtcggggc agccggtacc ttaaccctgc accttaaccc
     1681 tgtgccttaa cggtatatag gaggcacgat aggtgcgagg catccgcatc tatcttgatt
     1741 cctaagccgt tttaccttcc tgaaatgaga acttttccat actttccctt tacaattcct
     1801 gtatagatcc ttacaatgtg ttctgctgtt ttatttatat atttctttct acttaatata
     1861 ttccttttcc agccagccgt tatccacatc caatctaact atattttatt tatgtctatc
     1921 gtatccgcac cgctccccgc cctttcctcc ctcatcatcc tcgcccatta ccacggcatt
     1981 gccgccaatc ctgccgatat acagcatgaa ttttgtactt ccgcacagag cgatttaaat
     2041 gaaacgcaat ggctgttagc cgccaaatct ttgggattga aggcaaaggt agtccgccag
     2101 cctattaaac gtttggctat ggcgacttta cccgcattgg tatggtgtga tgacggcaac
     2161 catttcattt tggccaaaac agacggtgag ggtgagcatg cccaattttt gatacaggat
     2221 ttggttacga ataagtctgc ggtattgtct tttgccgaat tttctaacag atattcgggc
     2281 aaactgatat tggttgcttc ccgcgcttcg gtattgggca gtttggcaaa gtttgacttt
     2341 acctggttta ttccggcggt aatcaaatac cgccggttgt tttttgaagt attggtggtg
     2401 tcggtggtgt tgcagctgtt tgcgctgatt acgcctctgt ttttccaagt ggtgatggac
     2461 aaggtgctgg tacatcgggg attctctact ttggatgtgg tgtcggtggc tttgttggtg
     2521 gtgtcgctgt ttgagattgt gttgggcggt ttgcggacgt atctgtttgc acatacgact
     2581 tcacgtattg atgtggaatt gggcgcgcgt ttgttccggc atctgctttc cctgccttta
     2641 tcctatttcg agcacagacg agtgggtgat acggtggctc gggtgcggga attggagcag
     2701 attcgcaatt tcctgaccgg tcaggcgctg acttcggtgt tggatttggc gttttcgttt
     2761 atctttctgg cggtgatgtg gtattacagc tccactctga cttgggtggt attggcttcg
     2821 ttgcctgcct atgcgttttg gtcggcattt atcagtccga tactgcggac gcgtctgaac
     2881 gataagttcg cgcgcaatgc agacaaccag tcgtttttag tagaaagcat cactgcggtg
     2941 ggtacggtaa aggcgatggc ggtggagccg cagatgacgc agcgttggga caatcagttg
     3001 gcggcttatg tggcttcggg atttcgggta acgaagttgg cggtggtcgg ccagcagggg
     3061 gtgcagctga ttcagaagct ggtgacggtg gcgacgttgt ggattggcgc acggctggta
     3121 attgagggca agctgacggt ggggcagctg attgcgttta atatgctctc gggacaggtg
     3181 gcggcgcctg ttatccgttt ggcgcagttg tggcaggatt tccagcaggt ggggatttcg
     3241 gtggcgcgtt tgggggatat tctgaatgcg ccgaccgaga atgcgtcttc gcatttggct
     3301 ttgcccgata tccgggggga gattacgttc gaacatgtcg atttccgcta taaggcggac
     3361 ggcaggctga ttttgcagga tttgaacctg cggattcggg cgggggaagt gctggggatt
     3421 gtgggacgtt cggggtcggg caaatccaca ctcaccaaat tggtgcagcg tctgtatgta
     3481 ccggagcagg gacgggtgtt ggtggacggc aacgatttgg ctttggccgc tcctgcctgg
     3541 ctgcggcggc aggtcggcgt ggtcttgcag gagaatgtgc tgctcaaccg cagcatacgc
     3601 gacaatatcg cgctgacgga tacgggtatg ccgctggaac gcattatcga agcagccaaa
     3661 ctggcgggcg cacacgagtt tattatggag ctgccggaag gctacggcac cgtggtgggc
     3721 gaacaagggg ccggcttgtc gggcggacag cggcagcgta ttgcgattgc ccgcgcgtta
     3781 atcaccaatc cgcgcattct gatttttgac gaagccacca gcgcgctgga ttatgaaagt
     3841 gaacgagcga ttatgcagaa catgcaggcc atttgcgcca accggacggt gctgattatc
     3901 gcccaccgtc tgtccaccgt taaaacggca caccggatca ttgccatgga taaaggcagg
     3961 attgtggaag cgggaacaca gcaggaattg ctggcgaagc cgaacggata ttaccgctat
     4021 ctgtatgatt tacagaacgg gtagaaggcg ggggatgggc gtgtaggctg cttttttcgt
     4081 aacgttctgc gttttcagac cgtcgtttac tgggtttact gactgaaatg aaaccaaagt
     4141 gcaggctgcc tgaaagactt tttctgtctt tttaggcagc ctgcacttct ttttcactca
     4201 tctagagacc tttgcaaaat tccccaaaat cccctaaatt cccaccaaga cattttaggg
     4261 gatttctcat gagcaccttc tttcaacaaa ccgcgcaagc catgattgcc aaacacatcg
     4321 accgtttccc gctattgaag ttggatcagg taattgattg gcacccgatc gagcagtacc
     4381 tgaaccgtca aaaaaaccgt tacctccgag accaccgcgg ccgtcctgcc tatcc
//
LOCUS       DAWFJW010000135         4388 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFJW010000135 DAWFJW010000000
VERSION     DAWFJW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.135"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(1..211)
                     /locus_tag="WGR19_001872"
                     /pseudo
     CDS             complement(1..211)
                     /locus_tag="WGR19_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(361..1368)
                     /locus_tag="WGR19_001873"
     CDS             complement(361..1368)
                     /locus_tag="WGR19_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0799344.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINCQLTEKGLKIEKASAAVVDATIIQTAGSKQRQAIE
                     VDEEGQISGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDVEGYIEKLHITPANAHECK
                     HLPPLLEGIAEGTTVYADKGYDSAENRQHLEEHQLLDGIMRKACRNRPLSETQTKRNR
                     YLSKTRYVVEQSFGTLHRKFRYARAAYFGLCKVSAQSHLKAMCLNLLKAANRLSAPVA
                     A"
     gene            complement(1433..2374)
                     /locus_tag="WGR19_001874"
                     /pseudo
     CDS             complement(1433..2374)
                     /locus_tag="WGR19_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
     gene            complement(2370..2545)
                     /locus_tag="WGR19_001875"
                     /pseudo
     CDS             complement(2370..2545)
                     /locus_tag="WGR19_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2767..4080)
                     /locus_tag="WGR19_001876"
     CDS             complement(2767..4080)
                     /locus_tag="WGR19_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0799345.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1048 a         1286 c         1096 g          958 t
ORIGIN      
        1 cgtgtcggca atcgacagcc cccagccccg tcccctgctg ctccgttccc aaaatacggt
       61 aaaacggatc gaggacgcgg gcgcgttcgg attcgggaat gccgttcccg ttatcttcca
      121 cccacacggc gagatatttc ccttcgtccg tgaaacccaa atctatcctg ccttcgggcg
      181 gcgtgtaacg taccgcgttg tcggcaaagg ttgagacctt tgcaaaattc ccttccctcc
      241 cgacagccga aacccaaaca caggttttcg gctgttttcg ccccaaatat ctcctaattc
      301 tacccaaacg cccccttaat cctccccgga tacccgataa tcaggcatcc gggccgcctt
      361 ttaggcggca acaggcgcac ttagcctgtt ggccgctttc aacaggttca aacacatcgc
      421 cttcagatgg ctttgcgcac tcactttaca cagaccaaaa taggctgccc gggcgtagcg
      481 gaatttacgg tgcagcgtac cgaagctttg ttcgaccaca taacgggtct tcgacaaata
      541 tcggttgcgt ttggtttgcg tttccgacag cggacggttg cggcaggctt tgcgcataat
      601 accgtccaac aactgatgtt cttccagatg ttgccggttt tccgcactgt catagccttt
      661 gtcggcatag acggtcgtac cttcggctat cccttccagc aaaggcggca ggtgtttgca
      721 ctcatgggca ttggcgggag taatgtgcag tttctcgata tagccttcca catcggtacg
      781 ggtatgttgt ttgtaaccga gtttgtagag gccgtttttc tttatccaac gggcatcttt
      841 gtccttactc ggtgtggttt ggccgctgat ttgtccttct tcgtcaactt ctatggcctg
      901 acgctgtttg ctgccggcgg tctgaataat ggtggcgtca acgacggcgg cggatgcttt
      961 ctctattttt aagccttttt cggtcagttg gcagttaatc agtttgagca attcggacag
     1021 ggtgtcgtct tgcgccagcc agttgcggta gcggcataag gtgctgtaat cggggatgct
     1081 cagttcgtca aaacggcaaa acaggttgaa gtcgatgcgg gtgatgaggc tgtgttcgag
     1141 ttcgggatcg gagaggctgt gccattgtcc gagcaggacg gctttgaaca tggacaacag
     1201 gggataggcg ggacggccgc ggtggtctcg gaggtaacgg gttctttgac gattcaggta
     1261 ctgttcgatc ggctgccaat caattacccg gtccaacttc aatagcggga aacggtcgat
     1321 gtgtttggca atcatggctt gggcggtttg ccggaagaag gtgctcatgg aaaatcccct
     1381 aaatgtcttg gtgggaattt aggggatttt ggggaatttt gcaaaggtct caggttttaa
     1441 tcagcgtata gatttccgtt tcgtcggcag acacttcgac atcgcctccg accgccacgc
     1501 cgatgtcctg gcgtttttcc aaggccagcg gcatcagttc ctgcaacact tggcggaaac
     1561 ggctttgcag accgaatgtc gttttcgtca gaggggtttc gtccgactgc gaacgcgcca
     1621 gtgccaaaag ctgttcgagc aggtgtttgt tgcgcctgat gctctgctgc aaaacggcag
     1681 gctgccgcgc tgcatcgggt gggagcgaca tattgttgag ccgttccgcc tgaagggaaa
     1741 gggctgtcat cggcgtacgc aattcgtgtg ccgcgtcggc gacaaaccgc tgacggtggc
     1801 ggatgtcttc atccgcacgt ttcaaaagca ggttgatggc ggttacgaac cctctgattt
     1861 cactgggaat attgtccaca ctcaaagcag acaggtcatt gattcggcgt tgttcgagac
     1921 tttgcgacaa tttgcggacg gggcgcatgg ctttgtgcgt aatccacacg gtcagcagta
     1981 tcatcagcgg cagtgccgcc agcaggggca acacgctttg ccgtgccgca tccgccgcca
     2041 aatcttcacg gtattcgttt tcctgcataa cggcaatccg tccctgttcg gtcgtgcgga
     2101 tatagacgcg gtaataatcg tcgtccccgt ccgcctgaag cgtgtgcaga ccgtccgcca
     2161 gatgcgcagg caggctgaca acagggtctt cctgctgcgg catctgtacc aaaatacgcg
     2221 tatcgccgtc gccctcgggc aaagtttcgg gtttggaatc gggggcgacg tacaatgccg
     2281 cctgacggag caggtcgtcc tgcaacgctt ccgtttcgtg gaaggtttcg tagtaggaaa
     2341 acatacctgc aagcattgcc agcggaacaa acatccaaac cgtcccgccc cggcaaaccc
     2401 aaatccagca gcatcaagtc ataaggctgg gcagcggcag ccgcgcgccg tttttgaccc
     2461 aatccaccgc atagccgccg tctttcaaac ttgccgacac cgcctccgca atcatcgcat
     2521 cgtcttccac cagcaaaaca cgcatcaact ttcccttcaa aataaaccgt gcctattcta
     2581 acaccccaaa attagccgca atttagcggc ctttacgctt gccggtattt ttcaaaactg
     2641 cggcacaaaa aaccgcgccg gcaactgcct tcagacggca ttggggcgcg attgcaacac
     2701 acgggcaggc aggcagagcc tgcgacagac cacaggaacg attcgggctt cagacggctt
     2761 cgccgtttac ggcagaggca cgattcctgc cgctatcgaa ctggccaata tcgccagcga
     2821 caaaccccac gcccagaaaa acgaataacg gatgtgtttg cccatcgaca atttcgccaa
     2881 acccaagccc atccacaaag ccggcgaaag cggcgtaaca aaagtgccga cgatactgcc
     2941 gatcaacatc gcataacctg ctgcttcagg cgccacgccc gcctgcgagg taatctgctc
     3001 cacaatcgga aacagtccga aataataagc gtccgtactc aaaaccaact caagcggaat
     3061 gcccaacaca ccgatggcaa tatgcagata aggcagcagc gcgtccggca ggatatgcac
     3121 aaggtctttg gaaatcgcgt ccaacatccc cgcacccttc aaaatcccca aaaacgtacc
     3181 tgccgccaaa ataatggacg ccatcatcac cgcgccgccg gcgtgggcat aaatccgctc
     3241 catctgttcc tgcgggctgc ggtaattcaa aagcaacgcc gccgttgcag ccagcataaa
     3301 tacataaccc ggcgggaaga tgcccgaaaa aagcaggctc atcgccgcca aaaacagcag
     3361 gacattccac caaaacagtt tcggacgcgc caatttttgt tcttcttccg acaaaggcac
     3421 cggctttatc aaatccgcca cggcgggcaa cgcgcccaac tcccggacaa tccgcctttt
     3481 ttcacgcaca cccaaaagca gggacagcgc aaggataaac actacaccga taatttgcac
     3541 cgtcaacaaa ggtttataca attcgcccac atctgcgccc aacacgcttg caacccgccc
     3601 agtcggcccg ccccacggca gaaggttaat caatcccgca ctggaagtca gcagcaaaaa
     3661 cagcaggtaa ggattcatat gcagacgctt gtaaagcggc aaaagggcgg ggacgaccaa
     3721 taaaaacgtc gtcgcacccg ccccgtccaa ctgcgccacc accgacacca agaccgtccc
     3781 cacactcact gccacgatat taccccgagt cagcttaatc aaaccgccta tcatcggacg
     3841 gaacagcccc acatcgttca tgattccaaa aaacaaaatg gaaaacataa acataatcac
     3901 aatctgcatc accgatttgg tgccgcccga ataaaattct tttaattggg atacatcaaa
     3961 ccccgccagc aacgccccaa acagcggcac caagattaat gcgatgatgg gcgacacttt
     4021 ttccgtcagc agcaaccata cgatgacccc gataatcagc agtccgataa acgtcagcat
     4081 catttctcct ttattttatt ttaaacagaa aaccgaccgt gcaggcaaaa ccgcccacag
     4141 acgcgggata agccttgcat tctacttttt tattttgaaa caagtcaatc ggtcatttcc
     4201 tcccatttac gcctgccgcc attcctgtat ccgtccgtca tttcacggcg gcaaccgata
     4261 cggaacaacc ggtaaatcgg tatcgggacg gcgcgggggc attcatcccg gtgcgccgat
     4321 tcaaacgaaa ccgcccctat cattgcggag cgcggggcgt gccgtacacg cgggatttta
     4381 tagtggat
//
LOCUS       DAWFJW010000136         4254 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFJW010000136 DAWFJW010000000
VERSION     DAWFJW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.136"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(128..547)
                     /locus_tag="WGR19_001877"
     CDS             complement(128..547)
                     /locus_tag="WGR19_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HEZ0799346.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            complement(544..1281)
                     /locus_tag="WGR19_001878"
     CDS             complement(544..1281)
                     /locus_tag="WGR19_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0799347.1"
                     /translation="MVKTADGYKAIAHIQTGEHVFAKDETSGKTGYKPVTTRYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            complement(1496..1855)
                     /locus_tag="WGR19_001879"
     CDS             complement(1496..1855)
                     /locus_tag="WGR19_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799348.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDN
                     YKVNWKINNGNLIQTTN"
     gene            complement(1858..3261)
                     /gene="mafB"
                     /locus_tag="WGR19_001880"
     CDS             complement(1858..3261)
                     /gene="mafB"
                     /locus_tag="WGR19_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0799349.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKA
                     AVSGDFADSYKKKLALSDSARQLYQNAKYREALDIHYEDLIRRKTDGSSKFINGREID
                     AVTNDALIQAKRTISAIDKPKNFLNQKNRKQIKATIEAANQQGKRAEFWFKYGVHSQV
                     KSYIESKGGIVKTGLGD"
     gene            complement(3265..4206)
                     /gene="mafA"
                     /locus_tag="WGR19_001881"
     CDS             complement(3265..4206)
                     /gene="mafA"
                     /locus_tag="WGR19_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0799350.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
BASE COUNT          907 a          899 c         1099 g         1349 t
ORIGIN      
        1 aacttaattc ccaatatcct gtttcaaatg aaggaaatag gtcgtctgaa aattaaacac
       61 ggtttcagac gaccttttaa tcgttaggtt tacaatctaa cgtaatccat tcttacttat
      121 agatcgatta agagaagccc aaacaagctt ccctgtagct gaggatattt gcttcctcat
      181 atctatttaa aattcctttt aaatcagaat tatatttttt gggcggaaaa ttaaccgata
      241 aataattctc tccatttttt ctgattttac cactgtgttc ttctatggct gccaataaat
      301 cctttgggaa atgatccaaa acaacgatgt ttatcgtggt atttcctgaa gcttttatca
      361 agtggtcgaa aaacagcatc ttatcctcac gttctatggc aacaatgtcg tttaaagcca
      421 aattcggtgc gaaaagagga atctcttgaa taagaaaatt tccatcaggc attatgtttc
      481 ctgacagtat ttcttctgca atattgcttt ccaaatcgta atagacgaca gtaagtttcg
      541 atttcatttg ttactcttct taatgttgca gcctctgcat aatacctgcc cgtttttagg
      601 tgtaccttga ccacccttag atttagggat gacatgatct acttgtcttt catttgaagt
      661 aggagaaata ttttttatag attgtttggc tggaattgtt tcaataccgc aattagcgca
      721 cttaacttga ccattatttt gaatcctatt caaatcaata acggcttctt ttcctgcttt
      781 agtaaagtct tttcctgccc gatcgttttt actaagccgt tctgcgcgaa gttttgcttt
      841 ctctaaatta tttgaaccgc catacggaca atcattatga acccaaaccc cttccgtttc
      901 cgccttatcg cctttgacaa agtaggtatg ccaatcggca acggtcagat tgtaggcttt
      961 gagcggtttt ggtttgacaa cgatgttgcg gacggtttgg gttttgccgc tttcggataa
     1021 cagccggctt cccgctttca aatcttccgc tttaatccat ttcccatccg aataaaacgg
     1081 atggatgcgg ttggaaatca gggtttggct gttgccgatg ccgtctgaaa cttcaatgta
     1141 aacggtttcc tggtacggat tgccgtatcg ggtggtaacg ggtttgtatc cagtttttcc
     1201 actggtttca tctttggcaa agacgtgctc tccagtttgg atatgtgcga tggctttgta
     1261 gccgtctgcc gttttgacca aggtgctgcc gtggaaggag caggtgtagg atttttaaag
     1321 aacttagttc tcaatatcct gtttcattca tcaaaatgcc gtctgaaagc tgaataccgc
     1381 ttcagacggc attttggtgg ttgggttttt aagccaacct acgcttactg aaaaccaaat
     1441 tgagtttcag acagttttta ggtttgggtg tccaatctaa cttatatttg tccatttaat
     1501 tagtcgtttg tatcaaattt ccattattta ttttccaatt tactttataa ttatcttcat
     1561 aataatctaa ttcaaaaaaa cctgatattt caatatccaa ttccattatt gttttaatac
     1621 atttttcaaa ataaataatg aaataagatt ttacgcatgc accaaaaaaa atatagctgc
     1681 tccaattaaa actatttgtc gggaaaaccc acccgctttt atatattttt gcagattctt
     1741 tctcttcgat attaaaggga caattattcc aaaaattatt aatgttttct tggatgattt
     1801 ttatatcatc gtcagagcat tcaatccatc cttttatgga aacatatgat gccatgttta
     1861 atctcctaaa cctgttttaa caatgccgcc ttttgattca atatatgact taacttgtga
     1921 atgaacaccg tatttaaacc aaaattctgc acgttttccc tgttggtttg ctgcttcgat
     1981 ggttgcttta atttgctttc tatttttttg atttaagaaa tttttaggtt tatctattgc
     2041 tgaaattgtt cttttggctt gtattaaagc atcattcgta acagcgtcaa tttctctgcc
     2101 gttaataaat tttgatgaac catcagtttt tcttctaatt aaatcttcat aatgtatatc
     2161 tagagcttct ctatactttg cattttgata taactgtctc gcactatcag acaaagccaa
     2221 tttcttttta taagaatcag caaaatcccc gctaaccgca gccttccctg gttttgccgc
     2281 ctttgccaac ttcgcgactt tggctgctgc ggcaacgttg aagacggctt cgacggtttc
     2341 ggcggcattg ggattttcct gtatccaccg gtcaacggct tcgcgcgtat ttttttcaaa
     2401 gcccgccacg ctgcccaagc cgccgatgac ggcgaatttg ccctcggcgg gcaagggggc
     2461 gatgttgcgc attgcggctt tgtctatggc atagcgcgtt ccgtacagta tgtcgcctat
     2521 gcccaaggct tcgcccgcgc tgataaaggg gttgagcgcg ccggcggcga cgccgttgat
     2581 aaactccatg ctgttgcccc agcggtcgag cttggcattg tgctcgaaca tttttctgtt
     2641 ggcttcatcg gcgcggtcgg agaaattgct gccgaggttg ctgtaattgt cggatatgcg
     2701 ttgccggatg ctgcgggtgt cggtcggatt gagtttgatg ctgcgtgctg tgccgttgac
     2761 gtgataggtg tattcgtcgc gtgcgcccgt aggtttgggg taattgccgc ccttcgggcc
     2821 gtcgtaggca tcggcgggat gatgttcgtg tccttcccag ttgagccggt acacggtaaa
     2881 gccttcgtca acgttgcctt gttctttgct tgcgctgtcg gcggcgtggt tgtcgaaggg
     2941 ggcgtgttct tcgtgtccgt gtccggaaaa gcgggtgtgg tagccgattg tgccgttgat
     3001 gtttgcctgt tggatgagca ggttgcccat ctggtgggta tagtcttgga tgacgttgat
     3061 ttgaccggtg cggtcggaaa cgctgccgcg cgggtcgccg aagaggtggt atttgcctcc
     3121 gggttcgtag tgctgccgtt gggcgttatc ggtaatgaac ggatcttgcg ccaagtccgc
     3181 cgcgagggcg ggctgtccga aggcggcggc cgctacggcg caggcggcaa ggaggtttgt
     3241 cagtctgcgc agcggtttca cggtttatcc tcctttgcgg cggcggatga cttcgttgcc
     3301 gacatcgggg tttttaccgt tgttttgttt gaagtcggga cggttttggg cggttgtgtc
     3361 accgtagggg gtgatgtcgg agaaatcgac catcaggcgg tctgaggctt tgacggtttt
     3421 gccgacgctg taaggtccca tccagagggc gtattgttct tggtattggg attcgtaggc
     3481 ggcggtttta ggggcaatca gcagtttccg gctgtcgcgg tcaacggcga aatattcgag
     3541 cttggtttgg gctttaaggg tttcggcgtt gtagaggtgc agttcggtgc ggctgcggac
     3601 ggtgccgaat acgtcgacgg ttacgaatac gtcggtgtcg gcgtattcgg gcggtacgac
     3661 ttcgatgccg cgcaggtaga agacggtttg gatgaggttg gtcaggaagg aaacgtcgcg
     3721 ggggttggcg agcagggttt cgttgcggta gtcgcccgtg ccgttgacgg acagtccggc
     3781 ggagcgttcg cctttgcgtc cgctgtttct agtcagggcg gcggcggggg cgttcaaaag
     3841 cgatgtggaa gtggttacgc tggagagcgc gtcggatttg gtggtggcgg tagtgtcgta
     3901 ggcggggtag ctgtatcggg tggcactttc ggggttgttg tggtagccac cgcgtatcag
     3961 tgcgtcgata gagtagcgtc cgccgcttat gttgcccgaa ccttggtcgc ccataacgga
     4021 gacgtaaagg gcggctttgc gtcctttcag ggcggacaaa tccatttctt tgacggcggc
     4081 gcgggacgat gcggcgacga gttcttgttc gacggcaaag cgtttgccgc cgccgtgggc
     4141 gggtatgccg gtcagtgtgc cgcaggcggt taggacgagg gggatgagga ggagcagggt
     4201 tttcatggca gggtttgttt tgttttgaac gagtttgagt gtaaatggat ttta
//
LOCUS       DAWFJW010000137         4213 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFJW010000137 DAWFJW010000000
VERSION     DAWFJW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.137"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(1..143)
                     /locus_tag="WGR19_001882"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="WGR19_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(271..1521)
                     /locus_tag="WGR19_001883"
     CDS             complement(271..1521)
                     /locus_tag="WGR19_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0799351.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1588..1783)
                     /locus_tag="WGR19_001884"
                     /pseudo
     CDS             complement(1588..1783)
                     /locus_tag="WGR19_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="WGR19_001885"
     CDS             complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="WGR19_001885"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0799352.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3945..4213)
                     /gene="grxD"
                     /locus_tag="WGR19_001886"
                     /pseudo
     CDS             complement(3945..4213)
                     /gene="grxD"
                     /locus_tag="WGR19_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
BASE COUNT         1044 a          989 c         1014 g         1166 t
ORIGIN      
        1 atctcggagg taacgggttt ttgacggttc aggtattgtt cgatcggctg ccaatcaatc
       61 acctggtcca acttcaatag tgggaaacgg tcgatgtgtt tggcaatcat ggcttgggcg
      121 gtttgctgga agaaggtgct catggaaaat cccctaaatg gcttggtggg aatttagggg
      181 atttgggggg attttgcaaa ggtttccgcc tgaaacatta tgagatttca ggcggcattg
      241 gattgcttgg cggaatattt ttaaaaaggc ttacgcgccg taaacggggt atttattgca
      301 caaagcagtt acttgttcgc ggactttggc gaggttggct tcgtcttcgg ggttagacaa
      361 tacgtcggca accaagttcg ccaatacgcg cgcgtcggct tcgttaaaac cgcgtgtggt
      421 catggcggcg gaaccgatgc ggatgccgga ggtaacgaag ggtttttccg gatcgttcgg
      481 aatggcgttt ttgttgacgg tgatgtgcgc tttgcccaaa gcggcttcgg cggctttgcc
      541 ggtgattttc atcggttgca ggtcgacgag gaaaacgtgg ctttcggtgc ggccggaaac
      601 gatgcgcaaa ccgcgtttga ccagttcttc cgccatggcg gcagcattga ttttcacttg
      661 ttttgcgtat tgtttgaact cgggttgcaa cgcttcttta aacgctacgg ctttggcggc
      721 gatgacgtgc atcagcggac cgccttgcag gcttgggaag atggaagagt tcaacgcttt
      781 ttcgtgggta ttgtcgcggc acaaaattac gccgccgcga gggccgcgta gggttttgtg
      841 ggtggtggtg gtcacgaagt cgcagaatgg cacggggttg ggatattcgc caccggcaat
      901 cagtccggcg tagtgcgcca tgtcgacaaa gaggtatgcg ccgactttat cggcgatttc
      961 gcggaatttt gcccagtcga tttgcaacgc gtaggcagac gcaccggcca caatcatttt
     1021 aggtttgtgt tcgagagcca agcgttccac ttcggcataa tcgagcactt cgttttcatc
     1081 caaaccataa gtaacggcat tgtagagttt gccggaaata ttaacgctcg cgccgtgggt
     1141 caggtggccg ccgtgcgcca aagacatacc caaaatggtg tcgcccggtt ttaaaacgga
     1201 agcgtacacg gcttggttgg cttgcgagcc ggagtgcggt tgaacgttgg cgtattgcgc
     1261 gccgaacagt tttttgacgc ggtcgattgc caattgttcg acaatatcga cgtattcgca
     1321 gccgccgtag tagcgtttgc cggggtagcc ttcggcgtat ttgttggtca gctgggaacc
     1381 ttgcgcttcc attacggcgc agctgacgta gttttcagag gcaatcagct cgacgtggtc
     1441 ttgctggcgt tggtcttctt gggcaatggc tgctgccaaa tcggggtcgt attgtgcgag
     1501 ggtaacgctt tttgaaaaca tgttctcggc tcctttgtgt aatcagggta tcatgagtgt
     1561 tttttgtata aaaaaatact tcaaaaccta aggcagatag tccataatgc gtaaattttc
     1621 tttggcatta tcaggtaatt tatttaacat gctggttttt agcgtctcat tacctttatg
     1681 ttagtacaag gctacccaaa ggcaatgcat caaatggtaa attttcggca gtttgtgcaa
     1741 aatcaatcaa ataaccagca ccaaccacat ctttatcagc cataacccgc tgatacacca
     1801 cttgccaaga caactctcct gccccaccat gtcttaaaat cagatgaatg gcttgtaatg
     1861 ctgtgtttgg cgtctcacaa attgcgaaga tctgctcaga agcttgcttg atggcttgta
     1921 tttttgtttg attactcaca atttccccct attttaatat taacttaatg cggtcaaatt
     1981 cacaaaatac aagcttcacc tctaatcacc catcactcga ccttctcggc gtggatcggc
     2041 accaccaacc agcctgcttg gctcgataat aatggcttga acacctgaat ttagctcacg
     2101 cacgtcagtc ttatagccca aatcatttaa tgcttgttgc cactggaggg cggttgtacc
     2161 cgtttctagt tcatagctac caaagcgatt taataaattg ggtgcactga tggcatcttg
     2221 gatatccata ttccagtcac tatgtgccac aatcgtctta gcgacatagc caatgatacg
     2281 gctaccacct ggggagccga ttgccatata aggcttgcct gctttaaata cgatggttgg
     2341 tgccattgag gagcgtggtc tcttgccggg ctcgacacga ttggcgacct gtttgccctg
     2401 ctttattggc tcaaaactaa agtctgtcag ctcattattc agcaggtagc catttgccat
     2461 caaagttgag ccaaacgcat tttcaatgga agtcgtcatt gatagcacat tgcccgcctt
     2521 atccacaatt gatatatgac tggtagaagg taactcaatc gcttgtgagg acacccactc
     2581 atgaataaaa tcgcctgcag atacgctagg caatgcctta tccgactgct caagcagctg
     2641 gctgcgatgt tttaggtagt ctttagaaat caactggcga atgggtactg gtacaaaatc
     2701 agggtcgccc aaatatacat cacgatccgc aaacgcaagc ctagaagcgt cgcccaagag
     2761 acgtaaacct tcagcatcat accccacctg attgggtgaa aattcattta aaatccccaa
     2821 aatctgaccc acagcaatcc cacctgagct tggtgcaccc ataccgcata cttcataaat
     2881 acgataagtc acacaaacag gcgggcgttc caccacttga taatcagata aatcttgtaa
     2941 ggataattga ccggggttat ccttagcatt ttggacaact gaaacgatat tttgggcata
     3001 tttaccagta tgcagagctt ttgcaccttg agctgctaac gcctgaacac tgtcagcaaa
     3061 ttctaaattt ttcagcaagc tgcctgcttg tagcggcaca ccattcggca aaaaataagc
     3121 ggctgttttt ggatagcgtg ccaaatgctg ctgattttgc tcaaccgaga tggcaagcct
     3181 tggcgacacc tcaaagcctt gttttgccaa gcggatcggc gtatcaaata attttcccca
     3241 aggcaataca ccgtatcgct gatgtatcgt ctccatcagt ttagggatag caggcgtacc
     3301 caccgagcga ccaccgacca ccgcttccat aaatttcaat ggttgaccat ctttatccaa
     3361 aaataattcc ggcgtcgcac gcatcggtgc cgtctcacgc ccatcaaatg tggtcaatgt
     3421 tttggcggta ttatcccaat acaacacaaa tgcaccaccg cccaagcctg acgactgtgg
     3481 ctctaccaag cttagtgtcg tctgcaccgc caccatcgca tctgcagcgc taccgccttg
     3541 ctttaagata tcatagccag cttgtgttgc taatggattg gctgacgcta ccataaaatc
     3601 acttgcaatc acctgctttt gttcggtcag tcccgttgca tgttcaggcg tgtgagcgtc
     3661 tgcacctgtg atgacagcag aatgagtatt aaccttacct tgattggcat ggatgacttg
     3721 acatccagag attgtcatag acattatcaa tgcagtcaat aaatatgttt tagccacaag
     3781 cactccttcg cctgagtttg attgatgatt catacaaggc atgctgatta ttgtatttaa
     3841 tatggctaaa taattcaatc caaactatca atcttgacca tcaaaaaaag accgctaatg
     3901 tcatcagcag tcttttttga tatttatttt aagatattaa gtaatcagac ctttgggcta
     3961 tgctcttcaa tgagtggttt tagctcacct gattggtaca tttgtaggat aatatcacta
     4021 ccaccgatta actcaccatt aacccaaagc tgtggaaagg ttggccgatt ggcgatgagt
     4081 ggtagagtac tgcgaatttc tgggttttct aggatattaa caaaagcaaa gggtctgcca
     4141 attgggtcag cacctctact gcacgcgctg aaaatccaca ttggggaaac tggggcgtgc
     4201 ctttcatata tag
//
LOCUS       DAWFJW010000138         4177 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFJW010000138 DAWFJW010000000
VERSION     DAWFJW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.138"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            215..1732
                     /locus_tag="WGR19_001887"
     CDS             215..1732
                     /locus_tag="WGR19_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0799353.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPALAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNQNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1793..3673)
                     /locus_tag="WGR19_001888"
     CDS             complement(1793..3673)
                     /locus_tag="WGR19_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0799354.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDSALQKGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAENWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRVENGRCALLDRHLCRLKTSAQALN
                     LPLPDGCENQIKQYIARLPDGAFRIKALLASDGISLSRAVLNRLTDKQRVIISPTILP
                     AQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          860 a          953 c         1346 g         1018 t
ORIGIN      
        1 ttccgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
       61 tataaatgtg aaatccgccc tttgaaaatc gggcggcggt tttttgcctg cccgctttca
      121 gacggcaaag ccggttttca cgggtttctg cctgtttttc ggatggtttg acgtgcttcg
      181 gcggcgtgtt tgccggaaag gtaaatgaca gggtatgttg tatttcagat acggcttttt
      241 ggttgtttgg tgtgcggcag gtgtttctgc cgcctatggg gcggatgcgc ccgcgatttt
      301 ggatgacaag gcattgttgc aggtgcagcg gtcggtgtcg gataagtggg cggaatcgga
      361 ttggaaagtt gaaaatgatg ccccgcgcgt ggttgacggg gattttttgt tggcgcatcc
      421 gaaaatgttg gaacatagtt tgcgcgacgc gctcaacggc aatcaggcgg atttaatcgc
      481 ttcgttggcg gatttgtatg ccaagctgcc ggattatgac gcggttttgt acggcagggc
      541 gcgggctttg ctggcgaaat tggcgggaag gccggcattg gcggtggcgc ggtatcggga
      601 actgcacggg gaaaatgcgg cagacgagcg gattttgctg gatttggcgg cggcggagtt
      661 tgacgatttc cgactgaagt cggcagaaag gcattttgcg gaggcggcaa aattggattt
      721 gccggcaccg gttttggaaa atgtggggcg ttttcggaaa aaaacggagg ggctgacggg
      781 ctggcgtttt tcgggcggca tcagtccggc ggtcaatcaa aacgccaata atgccgcgcc
      841 gcagtattgc cggcaaaacg gaggccggca gatatgcagt gtcagccggg cggagcgggc
      901 ggcagggttg aattatgaaa tcgaggcgga aaaactgacg gcgttggcag ataatcatta
      961 tttgttgttc cgttccaata tcggcggcac gagctattat ttcagtaaaa aatcagctta
     1021 tgacgacggg ttcggcaggg cgtatttggg ttggcagtat aaaaatgcac ggcagacggc
     1081 ggggattttg ccgttttatc aggtgcagtt gtcgggcagc gacggctttg atgcgaaaac
     1141 aaaacgggta aacaaccgcc gcctgccgcc gtatatgctg gcgcacggag tcggcgtgca
     1201 gctgtcccat acttaccgac caaacccggg atggcaattt tcggtcgcgc tggaacatta
     1261 ccgccaacgc taccgcgaac aggatagggc ggaatacaat aacggcaggc aggacgggtt
     1321 ttatgtttcg tcggcaaaac gtttgggcga atcggcaact gtgttcggcg gctggcagtt
     1381 tgtgcggttt gtgccgaaac gcgaaacggt gggcggcgcg gtcaataatg ccgcctaccg
     1441 gcgcaacggt gtttatgccg gttgggcgca ggagtggcgg cagttgggcg gtttgaacag
     1501 tcgggtttcc gcgtcttatg cccgccgcaa ctataagggc gttgcggctt tctcgacaga
     1561 ggcgcaacgc aaccgcgaat ggaatgtctc gctggctttg agccacgaca agttgtcgta
     1621 taaaggtatc gtgcccgcgt tgaattatcg tttcggcagg acggaaagta atgtgccgta
     1681 tgcgaaacgc cgcaacagcg aggtgtttgt gtcggcggat tggcggtttt gaatggtgga
     1741 ataatgccgt ccgaactttg tggaaacagg ttcggacggc atttttgcgc gttcaggcaa
     1801 gggcggcggc aaatacgccg cgcaaggcgt tggagaggcg gatttcttcg gcttcttgca
     1861 gtgttttttg tgtgatgtgt gtttcgatta cttgatttgt ttgcaaatat ttttgcggtt
     1921 cgtccaatac ggcttggcgc attatgccgt ttaaaatgtc caaatctaaa gagggcgtaa
     1981 gccattgtcc gcgatgtttg acgaacacgt tgcttctgcc gccttcgagc aggatgccgt
     2041 ctgaattgaa aaacaggctg tcgaacgcgc cttgtgtttc ggcggtttgc cacgcttggt
     2101 cgaagacggt gcggcaggtg gttttgaagc ggcgcaggta gttttgtgcg ggcaggatgg
     2161 tcggcgaaat gatgacgcgt tgtttgtcgg tcagacggtt taaaacggcg cgggacaggc
     2221 tgatgccgtc tgaagcgagc agggctttga tgcggaacgc gccgtcgggc aagcgtgcga
     2281 tgtattgttt gatttgattt tcgcaaccgt cgggcagggg caggttgagg gcttgggcgg
     2341 aggttttcag acggcatagg tggcggtcga gcagggcgca gcgtccgttt tccacgcgca
     2401 gggtttcaaa aatgccgaag tcggggcgca attcgttgag gaaacgggct ttccagccgc
     2461 attcccgata ttcggcggca gggtcgctgt cgatgacgat gccggaaccg acaccgtaca
     2521 cgccccgata cggcggattg gagtcaaacg gatgcggcgt tgccccgcct tgccctgccg
     2581 ttcgggcttg cgcgtttgaa tccgggccgc cgatgcctga aacaatgccg tctgaagccg
     2641 aaacgggttt gagcgacaag gtgcggatga cgacgttgaa tatgccttca aaccccaacc
     2701 cgcccgcgca aggtttcagg tagccgatgc tgcccgtata aagtccgcgt gcttcggttt
     2761 cgagcgattc gataatctgc atactcattt ttttgggcgc gccggtgatg ctgccgcagg
     2821 ggaaggcggc gcggaggatg tcggcgaacg aggtgtgcgg cagggcttgg gcttggatgg
     2881 tgctggtcat ctgccaaacg ctgccgaaac gtgatacttt aaacggctcg ggcacgcata
     2941 ctttgccggt ttgggcgatt ttgccgaggt cgttgcgcag caaatcgaca atcatcacgt
     3001 tttcggcgcg gtttttcggg tcggcttgca attcggcggc gcggcgttcg tcttgtccgt
     3061 cgcccaaaat cggcgcggtg cctttcatcg gttcggtgct gatggtgccg tctgaagcga
     3121 tgttgaggaa gagttcgggc gagaaacaaa gcgtccacgc ggattgccct tccgcatcgg
     3181 gcaggtggga caaaacggca taggggacgg gctggcgcag gcggcggtag aggctgacgg
     3241 gattgccgta ggcttgcagg tgcaggcggg tggtgtagtt gatttgatag gtgtcgccgc
     3301 gccggatggc ttcgtggatt tggcggatat ggtcgaggta atcggcttcg gatacggagg
     3361 attgcggcgt ggaaatgccg gcggggaggc cgtctgaatg tcgggcaagc cagttttcgg
     3421 catcggtgtc ggcgcagtcg gcaaaccagt gcagggcaag attgccgccg cgttcggact
     3481 cgacccccgt cagcggcaaa ccgaatccgt agtctgcaaa caacaccgaa tgcagccctt
     3541 tttgccagcc cttttgcagc gcgctgtcca aagcatcgag ttcttcggga cggaaaaaac
     3601 ggctttccac atgattttga tagcgttttg cgcggccgct tacggcatcg tcaaacaggg
     3661 cgaaataagg catggcaatt cggggcaaat gttttgatta tacgctcttt ttacacatat
     3721 tttcagacga ttcggcaaat atcggcaaaa tgtaatttta tgtagagaaa gtggggggcg
     3781 aaggtgtaaa attgtgaaaa caaattccga tttccaacct gaaatacaat aaggagacct
     3841 ttatgacaga ccaccagctg caaccgtttg aaaacgtaga attaggcgaa aagcaagacc
     3901 agctccaagt atagtggatt aacaaaaacc agtacagcgt tgcctcgctt tagctcaaag
     3961 agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt
     4021 ctgcggctcc gtcgccttgt cctgattttt gttaatccgc tataaagacc gtcgggcatc
     4081 tgcagccgtc attcccgcgc aggcgggaat ctaggtctgt tcgatttcag ttatttccga
     4141 taaatgcctg ttgcttttca tttctagatt cccactt
//
LOCUS       DAWFJW010000139         4139 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFJW010000139 DAWFJW010000000
VERSION     DAWFJW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.139"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            136..2016
                     /locus_tag="WGR19_001889"
     CDS             136..2016
                     /locus_tag="WGR19_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ0799355.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            complement(3250..>4139)
                     /locus_tag="WGR19_001890"
     CDS             complement(3250..>4139)
                     /locus_tag="WGR19_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0799356.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          963 a         1160 c         1053 g          963 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcgga catcgacacc gatttattgt gggaagtatg cggcgaagag
      361 gaatttaccg ccgaagccat cgccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcaa ctttgattgc gctttacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaact ttaaaacaag cacttgccgc catcgaacgc
      541 aaaaaacagc aagacgcgca aatcgacgct tgggcggaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tatttcccca aaggaacagg cttccccgac cttccgcttc ctgaaatgcc tgacttaccc
      841 aaagccgacg ttaccgcctt ttccattgac gacgaatcga ctaccgaagt ggacgacgct
      901 ttaagcctga ccgatttggg caatggcata aaacgcgtcg gcatccacat tgccgcgccg
      961 tcgcttgcca ttaagcaagg cgacaaaatg gaaaaaaaca tcatggaacg cttaagcacg
     1021 gtttattttc ccggcggcaa aatcacgatg ttgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gtacataccg cccttccatc agcatttatt tcgatgtgga caacgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tcgctgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccaccgccct gcaaaaagcg
     1321 cgcggcaaat acgaacccga ccgcgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcggca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacgccaaat ggaagcctac tggagcctgg tttacctgca acagcaaggc
     1801 acgagcgagc tgacggcgac gattctgaaa gaagacctcg tccgcatcga aggtctgccg
     1861 ctagtaacac gcgcgaccgg tattccgttt gacgcgctgc ccaaatcgca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgatatgcc
     2221 gtcattcccg cgaaagcgga aatccagatt cttcggtaca gaaacttatc ggataaaacg
     2281 gtttctttag attctacgtc ctagattccc gcctgcgcgg gaatgacggc ataggggttt
     2341 ccgttttccc gataaattac cacaacccaa aatcccgtca ttcccgtaaa agcgggaatc
     2401 cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt cattcccacg
     2461 caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc cgcgaaagcg
     2521 ggaatccaga ccgttcggct tcagtttttt ggtttcgggc aacttctgca tcgtcattcc
     2581 cacacaggtg ggaatctagg tctgtccgca cgggaactta tgcgccgtca ttcccgcgaa
     2641 agcgggaatc cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt
     2701 cattcccaca caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc
     2761 cgcgaaagcg ggaatccaga cccttcggca cagaaactta tcggataaaa cggttgcttt
     2821 agattctacg tcctagattc ccgctttcgc gggaatgacg gcatagaggt ttctgttttc
     2881 ccgataaatt cctgttactt ttcgttgcta gattcccgcc tacgcgggaa tgacgatgga
     2941 aagattgttg ttgcttcgga taaatttctg cagttttaaa taaccggatt cccgcctgct
     3001 cgggaatgac ggcataggtt tttttgtttt tccgataaat taccacaacc caaaatcccg
     3061 tcattcccgc gacggcggga atccagtcct ttaaactcca gccattcccg ataaattcct
     3121 gttacttttc gtttctagat tcccgctttc gcgggaatga tgaatgacgg tgtaaaagta
     3181 actcgaaatc caaaaagcca taccgcccgg atttttgccg atcggtatgg ctttttgctt
     3241 caaaccgcct tagcggatgt cgacatacgc gccggaatgc aggtcacgca acaggttgac
     3301 ggttgcctgt tcggcttttt gttggaagat gtattgccgc acggaattgc ggatacgttc
     3361 ctgaggtgtg ccggcatcgc gcacttcgtt caatttgatg atatgccagc cgaattgggt
     3421 gcggacgggc gcgccgacct gtccgggttt gagcgcgtgg acggcttctt caaaggcggg
     3481 aaccatcacg ccgtcggcaa accagcccaa atctccgccg ttgcccgcgc tcgcgtcttg
     3541 cgaatattgg cgcgctaggc tggaaaagtc tgtgccgctg cgggcctctc cgtagatttt
     3601 gcggatggtg ctttccgcgc cgacggcggc gttttcgctg tcggctttaa tcaggatgtg
     3661 ttgggcgcgg tattggcgca acggtgcgcc ttcgggcagg gtgatgcctt gtttttgcgc
     3721 ctgctcgagg aaggcatcga tttcagcttc gctcacgcgg ctgttctgca tcactgcctg
     3781 ctggcggact ttttcggcaa tgatgttgtc ggcaaaatcg cggcgttggg cggggctgag
     3841 gtttttgagg gcggaatttt ttgcgacgac ggcatcgatt tccgcttcgc ttgcttgaat
     3901 gttgcggcgt ttgcccgcct gtacaatcag ggattggttg acaagctgca tcagcacctg
     3961 tcgggacagc tcggattcgc ttatctgcgc ctctttgggc aggttggctt tggcttcggc
     4021 aacggcttcg gcaagccggc ggcgcgtgat gacttcgttg tcggcaacgg cggcaatgcc
     4081 gtctgaaaag cggataccgc cctgctgttg tgcgggtgcg gcttcttttt gcgccgtgg
//
LOCUS       DAWFJW010000140         4129 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFJW010000140 DAWFJW010000000
VERSION     DAWFJW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.140"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            43..675
                     /locus_tag="WGR19_001891"
     CDS             43..675
                     /locus_tag="WGR19_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0799357.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WGR19_001892"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WGR19_001892"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0799358.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKSLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4065
                     /locus_tag="WGR19_001893"
                     /pseudo
     CDS             2198..4065
                     /locus_tag="WGR19_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
BASE COUNT          942 a         1130 c         1066 g          991 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttctcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgccgggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcagcc
      601 tctttgttcg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttctcagag tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgcccaa actggcagaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aagccttcag gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccacatggtc gcctatcccg acgtcgattg ggaaacccac tgccgcaact
     1441 tcgtcgaaat cagccttggc ctgaccttca acccctacac catccaaatc gagccgcacg
     1501 actacatggc tgaattcttc caaaccctca gccgcatcaa caccatcctg atcgacttta
     1561 accgcgacgt ttggggctac atttctttgg ggtacttcaa acaaaaagtc aaagcgggcg
     1621 aagttggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgagg
     1681 gcaaccttgg tatggcaaac gccgtattgg gttttttggc ggaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcccacctgc gcggtctgaa caagctcgaa cctaaccccg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgccg ttacggcgtg gccaatcctt acgagaagct gaaagacctg acgcgcggca
     1981 aaggcggcat cacgcccgaa gtgctgaaag gctttatcgg attgctggaa atccccgccg
     2041 aagccaaagc caaattgctt gagctgaccc ccgcgctgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacaggggc gatatttatg caagctgtcc gatacagacc
     2221 ggaaattgac ggattgcggg ccgtcgccgt gctatccgtc atgattttcc acctgaataa
     2281 ccgctggctg cccggaggat tcctgggggt ggacattttc tttgtcatct caggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttattgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggca gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgctg caaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcac tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgcct tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcggaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggt tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtgga tacgacactg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt ccaagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttgattaaag atattcccaa tgtgcattgg
     3901 gtggacgcac aaaaatacct gcccaaaaac acggtcgaaa tacacggacg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcctattata tggggcggga attccacaaa
     4021 catgaaagct tgctcaagca ttcacacggc aacgcattgc agtagcctgc cttcttgtcg
     4081 gatattgcct ttggcagcct atgccgctgt ttgcccttcg gggcggcgg
//
LOCUS       DAWFJW010000141         3929 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFJW010000141 DAWFJW010000000
VERSION     DAWFJW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.141"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(56..271)
                     /locus_tag="WGR19_001894"
     CDS             complement(56..271)
                     /locus_tag="WGR19_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799359.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(275..523)
                     /locus_tag="WGR19_001895"
     CDS             complement(275..523)
                     /locus_tag="WGR19_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0799360.1"
                     /translation="MDRLKAAYLSARPDMVLLLRYTEMGLNQAEAAKLLGIAPSNVRA
                     RLKQLADLGLTEYRPDPKYRNRHALAAAHGQQSLGLEG"
     gene            complement(781..966)
                     /locus_tag="WGR19_001896"
     CDS             complement(781..966)
                     /locus_tag="WGR19_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799361.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(1239..1406)
                     /locus_tag="WGR19_001897"
     CDS             complement(1239..1406)
                     /locus_tag="WGR19_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799362.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1643..1885
                     /locus_tag="WGR19_001898"
     CDS             1643..1885
                     /locus_tag="WGR19_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799363.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            1929..2120
                     /locus_tag="WGR19_001899"
     CDS             1929..2120
                     /locus_tag="WGR19_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799364.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            2117..2533
                     /locus_tag="WGR19_001900"
     CDS             2117..2533
                     /locus_tag="WGR19_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799365.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            2710..3114
                     /locus_tag="WGR19_001901"
     CDS             2710..3114
                     /locus_tag="WGR19_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HEZ0799366.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCTECSIPEL
                     ERVVEDLRQHGFTPKKTAGRRPNRRSSADPMMRKIEALLLDNGWSWNYAQGTAKKMFK
                     VDRVEWLSDGNMHKLVAALQISANRKKKEKTG"
     gene            3111..3590
                     /locus_tag="WGR19_001902"
     CDS             3111..3590
                     /locus_tag="WGR19_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799367.1"
                     /translation="MSLNWEMTEQDFEAVKHLLPHSVVAMITVIGLEAAFHMVKVWGG
                     TNYPISNRRRNTRQSRILHAQLVEDIGEEAAGRLERAYVGQPFLAIPRCWDAMRELRN
                     RFIRRQYDAMSAEGLSDLFIVRELVLAHRLSARNIRYILKEADREAAARAQTDLFVA"
     gene            3753..>3929
                     /locus_tag="WGR19_001903"
     CDS             3753..>3929
                     /locus_tag="WGR19_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0799368.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMP"
BASE COUNT          968 a          834 c         1028 g         1099 t
ORIGIN      
        1 cgtctgaaac ggggtttaaa acctgtttca gacggccttt tttcatgccc tagattcagg
       61 ctgctttctc ttcctgctcg tacacggcgt tgtagaaagc ggcggacagc ttgtcggctt
      121 tctcggaggc ggtttcaatc acggcagaca aaccgtcttc gattccttcc atgtccatct
      181 ccaaaacttt cagatggttg agtgtgaaca ccagcagatt cagggctttg aggctgtctt
      241 ggtcgaaagt cagggtatag gtggtattca tggcttaacc ctccaatccc aaagactgtt
      301 ggccgtgtgc cgccgccagg gcgtggcggt tgcggtattt cgggtcgggg cggtattcgg
      361 tcagacccag atcggcgagt tgtttgaggc gcgcgcgcac gttggacggc gcaatgccca
      421 agagtttggc ggcttccgcc tgattcagtc ccatttcggt gtagcgcagc aggagcacca
      481 tatcggggcg ggcggaaagg taggcggctt tgagcctgtc cattgcgctc tgtaaactct
      541 ttagagcatt ccaaattttg aatggcgcgt aacacatctt tgtgcttctt gccgaaattg
      601 tttgatacgg cgagcgaggt tgtaaccgga cggtcgccgg agatttgaac aagttgggtg
      661 ttgttggtat tcatgataga atagcctctc ttttagattg catttaaaag acacgagaaa
      721 gaacgttgca gcgttgcttt cccactgttc ccccgatgtt ccaccatcgg gggaattttc
      781 ttatttcatt ttttccttca tgactgattt aacccagttt gaaaagtcgg gaatttcttt
      841 ttgcataaaa tccaccaact ctttctctct ttccaaatgg aaagaaactg ttttcatcag
      901 ccgcttttgg tcatactttc gctgatattc agcttgtttt gcttttagcg ttttttcatc
      961 aggcataggc aatccttgat ttttaaaatc gttgtagtaa aatggcttgc agaaaggttg
     1021 gcgaaagggg cgcggtgttc tgccacccct tgtgtttaat caacttgctt agtaagccgg
     1081 actacagctt aaaagcagaa tgatgaaaaa aacaactttc gccagttttg tcatttccac
     1141 aagcctcctt tctatccgat aagcccccga agttgccgct tcggggcttt gctttatcag
     1201 ataggcgtat tatctagtat ttctagaatt tagtcaagct attttttgat tttttctttg
     1261 atgattgatt taacccattg ggaaaaatcg atattttggg catattccaa aagttctttt
     1321 tccgtttcgg cattgaacga gactttctta atcacccgtt tctgctcgta acgctttcgg
     1381 ttttctgcca gtttttcatc aaccataggc aaactccttg attttttaaa ccgtattttg
     1441 taagattggg actggggaca gcggcgaccg cccccagctg gtcagtcttt agtaggcatt
     1501 agcacttgcc aacatgaaaa ctgcgaccaa gatgagaatt tgaatagtgc gtttattcat
     1561 cttctttctc ccagttgagc cccgcttcgg tggggctttt cccgtatcgg ccctcactac
     1621 gccgatgaat tgaattataa acgtggacac gttaaaagtc aaccctttcc ccaacaaaat
     1681 caaagaaaaa tgccgtctga aaacagctcg aatgctgttt cagacggcat tttttgttgt
     1741 cgggcatcaa tgccctgcca tttgtgcggc gttgcggtta tcaagatatg gtattttagt
     1801 gtgtattttt agaagaaatt taccatatat agtattaaaa tattatacgg ttgttttata
     1861 tggatttgat tttcttgaaa aataaatccc atatgctaat atttgcacta tttgggcgag
     1921 gttgaaaaat gaataccgtc aaagaatata cggcagtccg cgaacggctg ctcaatgctg
     1981 ccgattattt ggaagaagtc cgtaaagacc gtaagacggg caatattgcg tccgtggaat
     2041 ttgtgccgcc gaaaataggg gctcggggtt acggcaaatt caaagtccgt tacaaaacct
     2101 tagtggcggt tgatttatga gcggggaaaa atccgagaat ctgccggccg atgagcagca
     2161 gaaaatgctt caggagtttt tagaacttca aaaacaggat attgcagtca aacagaagga
     2221 gttggaaagc cgtaaggacg aaatccaatc tcacgaacgc attgcactgg ctaccatcga
     2281 agcgcaaaaa cagggtgaac agcaacatgg tgagattttc aaagaggtac acaaacgccg
     2341 tctgaatgcc gtgattgcta tttcttttct gattgccgcc gtgctgatta ctgctttatg
     2401 gcgcgataag gacagcgtcg ctattgaaat tgtcaaaatc ggcggggcgg tagctttggg
     2461 atattttgca ggaatcaaca gaggtaaagc gcaaaccttg gaaaagcaac ggcgcgaaaa
     2521 agacagtgat tagattgtag taaaaaaggc cgtctgaaat gttttggcga aactcgcaaa
     2581 gctcgttttc agacggcttt ttcatgcctg tccgtttcgt aaaaaaaaca gcggcttact
     2641 acaatatata gtattttata tgtataatat gcatcaatta atcaatatat tgtgttttat
     2701 gggtttgaaa tgcgccgggc gttgattgcg aaaattaaga ttgctcaaaa ggagctgggc
     2761 ttggatgacg gtacctatcg agcggtgtta gagcgggtga cgggcaagcg gtcgtgtacg
     2821 gagtgcagta tccctgagct ggaacgcgtg gtcgaggatt tgcgccaaca tggttttacg
     2881 ccgaaaaaaa cggcaggccg acggccaaac cgccgctctt ctgccgaccc gatgatgcgc
     2941 aaaatcgaag ccctgctgct ggataacggc tggtcttgga attatgcgca gggtacggcg
     3001 aaaaagatgt ttaaggttga ccgcgtggaa tggttgtccg acggcaatat gcacaagttg
     3061 gtggcggctt tgcagattag cgcgaaccgc aagaaaaagg agaaaacggg atgagtttga
     3121 actgggagat gacagagcag gactttgagg ctgtgaaaca tctgctgccg cacagtgtgg
     3181 tggcgatgat tacggttatc gggctggagg cggcgtttca catggttaag gtttggggcg
     3241 ggacgaatta tccgatttcc aaccgccgcc gcaatacgcg tcagagccgc atcttacacg
     3301 ctcaactggt cgaggacatc ggcgaagaag cagcgggacg gttggagcgt gcttatgtcg
     3361 ggcagccttt cttggcgatt ccgcgctgct gggatgcgat gcgcgaactt cgcaaccggt
     3421 tcatccgccg ccaatatgat gcgatgagcg cggaaggttt gagcgatttg tttattgtgc
     3481 gcgagctggt attggctcat cggctgtctg cgcgcaatat ccgatacatt ttgaaagagg
     3541 ccgaccgcga ggcggcggca agagcgcaga cggatttatt tgtagcttga ttgtgttgtg
     3601 tgtttcttgt gagtggacct ttgccctgct ttttgcaggg cttttttttg tctgcggaac
     3661 agtaagtgca tttgtgccga ctgtcttatg ccgtctgaaa aggttaaata aggttttgaa
     3721 aataaattgt gatttgattt tcggagatgt ttatgggcaa aactgtaact ttaaccgccg
     3781 gccacagcaa caccgacccg ggtgcggtca acggcagcga ccgtgaggcg gacttggcgc
     3841 aggatatgcg caacatcgtg gctgctattt tgcgcgatga ctacggtttg actgttaaaa
     3901 ccgacggcac aggcaaaggc aatatgccg
//
LOCUS       DAWFJW010000142         3892 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFJW010000142 DAWFJW010000000
VERSION     DAWFJW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.142"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..274
                     /locus_tag="WGR19_001904"
     CDS             <1..274
                     /locus_tag="WGR19_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PspA"
                     /protein_id="HEZ0799369.1"
                     /translation="NNTTQGACKGLCYSHSSYFAEVPEQYQRDASGEYVIRDGKKVET
                     EAWKDYKSIWGEPTEKDENGKYINKYIPKLINPNKPNGEKYEDKPF"
     gene            255..563
                     /locus_tag="WGR19_001905"
     CDS             255..563
                     /locus_tag="WGR19_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799370.1"
                     /translation="MKINHFNIALLFVSTMMLTGCFGHCADFNCTRLDPRWVKAVDTC
                     ETKNLTIVLQVLGEDLGYKSIIEVQDSKIGQHISSQCIYHSGKYDIHKDKQYEHLFAK
                     "
     gene            911..2836
                     /locus_tag="WGR19_001906"
     CDS             911..2836
                     /locus_tag="WGR19_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0799371.1"
                     /translation="MTVGYGFSAGGDYSQSKIRADHASVTEQSGIYAGEDGYQINVQN
                     HTDLKGGLITSTQKSEDDGKNTFSTGTLTFEDLANHAEYKGEAIGLGVSGSVAGDNPN
                     QNSRIYTVNQSGMSSTMGYGKDSDKQYATTHSGINTANITIRDNEKQQQITGISAEEA
                     KRAVKADVTTDNYAEHSGSLKNNFDKDKVQSEIDLQVKVTQEFDSNRKALRAEINKAA
                     DEKRQKAEDIRRENYIDGKNGYNTAESLALDKSADKLEKVAFYTDLALGGLYGIGDST
                     ALTYVTGSAVANPAIRAGTRPRQFWFEKCDGDSLYCSNRNYDDTADRKIYDKNGQEID
                     TKDPNYTGMVEIGDKRQISDLNEIEPNKVTGSIVTTNDGILNPQNDALKNGIKQQKWE
                     VDKEGILLIYNPPTGNSISELLYAAYDKTNDLLGGRLPLTSAQKANLELNQYAESKGY
                     KIDSNNHSRGGLTRSVSDKYSVRHNLPLPYNNVQFFGSAANVDDYTNYMNEYGRKDFN
                     ISATTHYTDFVGRTPLVALRSKHIVGGNEPTGGVEDKWFLYSHSSYFAEKPTPYLVNE
                     HGFYMDKNGDLIKDNSGNPIKMTEENKLEYSTKVPNPYLDDFEQKWIKGQTHNKVSGD
                     NPSLPVPVRTKKQQGER"
     gene            2836..3267
                     /locus_tag="WGR19_001907"
     CDS             2836..3267
                     /locus_tag="WGR19_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799372.1"
                     /translation="MKKYFFSVCLLLTGCGVLFDDPLTRFWNGGYFLFPDKEIASDGI
                     YKTSDTDLHYYESLSLEQRKRYLKFKPIKDKCEHKYSDRTKYDYATQMANIVRCFNEQ
                     GYQSETAFILNGHTITQKGSICTKDEPKQCDNVMRSYIVLF"
     gene            3309..3560
                     /locus_tag="WGR19_001908"
     CDS             3309..3560
                     /locus_tag="WGR19_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799373.1"
                     /translation="MKPIYFIIFSFLLTACVVNNDDYFAEPPTPYLINEHGFYMNKKG
                     DLIRDSHGNAIKMTEENKHNFPKESANPYYEKWIKQKRK"
BASE COUNT         1429 a          671 c          788 g         1004 t
ORIGIN      
        1 caataacacc acacaaggtg catgtaaggg gttgtgctat tcgcatagca gttattttgc
       61 ggaagtgcct gaacagtacc aaagggatgc gagcggagag tatgttatta gagatggtaa
      121 aaaagttgag acagaagctt ggaaagatta taagtcaata tggggcgaac cgaccgaaaa
      181 agatgaaaat ggtaaataca tcaacaaata tattcctaaa ttaatcaacc caaataaacc
      241 aaatggagaa aaatatgaag ataaaccatt ttaatatagc attacttttt gtttccacca
      301 tgatgctaac aggatgtttt ggtcattgtg cggattttaa ttgcacgaga ctcgatccac
      361 gctgggtaaa agcagttgat acatgtgaaa ctaaaaatct cacaatagtg ttacaagtat
      421 taggggaaga tttaggatac aaatctatca ttgaggtaca agacagtaaa attggtcaac
      481 acatttcttc tcaatgtatt taccatagcg gtaagtatga tattcataaa gataagcagt
      541 atgagcattt gtttgcaaaa tgatattacg ccaatgtttt acagaaaaac ggctatacct
      601 atacgggtgc ggacggcaaa acttataatt cgattgtgca taataaggat tttgtggaga
      661 acaaagggat acctttcttg ctagaaacca atgacaccac acaaggtata tgtaagggat
      721 tttgctatcc gcatagcaat tattttacgg aagtgcctga acagcaccaa aaggataaga
      781 atgggaatat tgttaaaaag aatggtgaac ctgcatcaaa ggcgtacaag tcaatgccaa
      841 aaacctaagt attcaaagcg tacaagatag agaaacttat caaagcaaac aacaaaacgc
      901 cagtgcacaa gtgaccgtag gctacggctt cagtgccggt ggcgattaca gccaaagcaa
      961 aatccgagcc gaccatgctt cggtaaccga gcaaagcggt atttatgccg gagaagacgg
     1021 ttatcaaatc aatgtgcaga accacaccga tttgaaaggc ggtttgatta cttccaccca
     1081 aaaatcggaa gacgatggca aaaacacatt ctcaacgggt acgctcacct ttgaagacct
     1141 agcgaatcac gccgaatata agggtgaagc gattggtttg ggtgtttcag gcagcgttgc
     1201 gggcgataat ccaaatcaaa acagccgtat ttataccgtt aatcaaagcg gtatgagtag
     1261 cacaatgggc tatggcaaag acagcgacaa acaatacgcc acaacccatt caggcattaa
     1321 cactgccaat atcaccattc gtgataacga aaaacaacag caaatcacag gaatatcggc
     1381 ggaagaagcg aaacgggctg ttaaagccga tgtaacaacc gacaactacg ccgaacattc
     1441 aggcagcctg aaaaacaatt tcgataaaga caaagtgcaa tcagagattg atttgcaggt
     1501 taaggttaca caggagtttg atagtaatcg caaagccttg cgtgcagaaa tcaacaaagc
     1561 agcagatgaa aaaagacaaa aagccgaaga tataagaaga gaaaattata ttgacggcaa
     1621 aaacggttat aacaccgcag aatctttggc tttggataag tctgccgaca aattggaaaa
     1681 agtcgctttt tacactgatt tggcattagg tggattgtat ggaattggcg atagcacggc
     1741 tttaacttat gtaacaggta gtgcagtggc taatccagcc attcgagcgg gtacaagacc
     1801 aagacagttt tggtttgaga aatgtgacgg cgatagtttg tattgttcta atagaaatta
     1861 tgacgatacg gcggatagaa aaatttatga taaaaacggg caagaaattg acactaaaga
     1921 tccaaattac actggaatgg ttgagattgg tgataaacgg caaatctctg atttaaatga
     1981 gattgaacct aacaaagtaa ctggaagcat cgttactaca aatgacggta tattgaaccc
     2041 acaaaatgac gctctcaaaa atgggattaa gcaacaaaaa tgggaagtag ataaggaagg
     2101 tattttgctt atctacaatc cgccaacagg caattctatt tctgaacttt tgtatgccgc
     2161 ttatgataaa actaacgatt tgttaggcgg tcgtttgcct ttaacttctg cacaaaaagc
     2221 gaaccttgaa ttaaatcaat atgcagaaag taaaggttat aagattgata gtaacaatca
     2281 tagtcgtggc ggtttaactc gaagtgtttc tgataaatat tcagtcagac ataatctgcc
     2341 tttaccgtat aacaatgttc aattcttcgg tagtgcggca aatgtagatg attacacaaa
     2401 ctacatgaac gaatatggta ggaaagattt taatatttct gccacaacac attacacaga
     2461 ttttgttggt agaactccat tagttgcatt gcgtagtaag catattgttg gtggaaatga
     2521 accaacaggc ggagttgaag ataaatggtt cttatattcg cacagcagtt attttgcaga
     2581 aaaaccaacc ccataccttg taaatgaaca cggtttttat atggataaaa atggcgacct
     2641 gataaaagat aattcaggaa atcctataaa aatgacggaa gaaaataaat tggaatattc
     2701 aacaaaagtt ccaaatccat atttagatga ttttgaacag aaatggataa aaggacaaac
     2761 tcataacaag gtgtcaggtg ataatccgtc tcttccagtt ccagttagaa caaaaaaaca
     2821 acagggagag cgataatgaa aaaatacttc ttttcagtct gcttgttatt aacaggctgt
     2881 ggggttttgt ttgatgaccc actaacaaga ttttggaatg gcggttattt tttgtttcca
     2941 gacaaagaaa tagcaagtga tggaatttat aaaacttcgg atactgactt gcattattac
     3001 gaaagtttgt cgcttgaaca aagaaaacgc tatctaaaat ttaagcctat taaggataaa
     3061 tgcgaacata agtattcaga tagaacaaaa tatgattacg ctacacaaat ggcaaatatc
     3121 gttaggtgtt ttaatgaaca aggctatcaa tctgaaactg cttttatatt gaatggacat
     3181 acaataactc aaaaaggtag tatttgcaca aaagatgaac ccaaacaatg cgataatgtt
     3241 atgcgtagtt atatcgtttt attttaattg aaatgcaact tgaaaattga caatacagga
     3301 gcaacaaaat gaaaccgatt tattttatta ttttttcttt tttattaact gcttgcgtgg
     3361 ttaataatga tgattatttt gctgaaccgc ctacaccata cttaattaac gaacacggtt
     3421 tttatatgaa taaaaaaggt gatttaatta gagatagtca cggtaacgca attaaaatga
     3481 cagaagaaaa taaacacaat ttccccaaag aatccgctaa tccttattac gaaaaatgga
     3541 taaaacaaaa acgaaaataa ataacaaagc aaacatagtg taggcaactt gttgcccacg
     3601 cgttaatgat aactttcagg cagcctgaaa gcctaaaacc acaaggaaag ctccaaaaat
     3661 gaacaaccgc ttacacaaag tcatcttcaa ccgcaacaca gcaagcgtag ggtgggcaac
     3721 ttgttgccca cgcagttcaa tcagatacac ataaaatgat gttttaatgt gaaatatttg
     3781 atttcaggct gcctgaaaaa taaaatgatg tggtgtgcat caaaccgcgt gggcaacaag
     3841 ttgcccaccc tacgcttgct agaccgaata gcaggtattt acatcacagg ca
//
LOCUS       DAWFJW010000143         3888 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFJW010000143 DAWFJW010000000
VERSION     DAWFJW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.143"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            519..1475
                     /gene="thiL"
                     /locus_tag="WGR19_001909"
     CDS             519..1475
                     /gene="thiL"
                     /locus_tag="WGR19_001909"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0799374.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1468..1953
                     /locus_tag="WGR19_001910"
     CDS             1468..1953
                     /locus_tag="WGR19_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0799375.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(2013..3689)
                     /gene="ettA"
                     /locus_tag="WGR19_001911"
     CDS             complement(2013..3689)
                     /gene="ettA"
                     /locus_tag="WGR19_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0799376.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          790 a          946 c         1085 g         1067 t
ORIGIN      
        1 ttagagaatc gttctctttg agctaaggtg aggcaacgct gtactggttt aaatttaatc
       61 cactatatcg gttgaaactc tgattttaag gcggtaggat gtgggtttgc ccatagaaag
      121 ggaatccttt ctgtatcaag ccctgaaagg gataattcat acaaattcac gcctttcccc
      181 ctcattggga aatggatgga atcgtgccag atgtgtgcgg cactgtatgc cggatatggt
      241 tttatcatca gcccttttcg gttgaaacct cgccagttgc agcgattgag cctaatcggt
      301 ggcggaagtt gccgctttgc attcggggtg gcgtgcagtg cggtgctttg atatgccgtt
      361 tgtgtgttga aacagggtgg tcggtgcata cgggtacggt atggccaaag ctaaaagtga
      421 aatacgctga aacactgaat gagccgcttt attgtttgta cggcctttgc tgccttgcta
      481 tgatttaaat tggattcgcc cgccggatat tttgggatat gaaagaattt gacttcatca
      541 aacggtattt gcaaacaggc acggataatg atgtcgtatt gggcataggc gacgatgcgg
      601 cgattgtccg cccgcgtgaa ggcttcgatt tgtgtttcag tgcggatatg cttttgaagg
      661 acaggcattt ttttgcagat gtcaaacctg aagacttggc ttggaaggtt ttggccgtca
      721 atatttcaga tatggcggcg atgggtgcga taccgcgttg ggtgttgctg agcgcggctt
      781 tgcccgaatt ggatgaggta tggctgaaac ggttttgcgg cagctttttc ggtttggcaa
      841 aaaagtttgg cgtaacgtta atcggcggcg atacgaccaa gggcgatatg gcgttcaatg
      901 taaccattat cggcgaattg ccgaagggta gggcgttgcg gcgtgatgcg gcggttgcgg
      961 gcgacgatat ttgggtgtcg gggcgtgtcg gtatggcggc ggcggctttg aactgccgtc
     1021 tgaaacggtg tgtgttgccg gatgatgtgt ttgccgaatg cgaacaaaag ctgctccgcc
     1081 ccgagccaag ggttgggctg gggcttgcgc tgttgccgtt tgcccgggcg gcgcaggatg
     1141 tttcagacgg cctcgcgcaa gatttggggc atatcctgac cgcttccggc gtgggggcgg
     1201 aaatttgggc cgattcgctg ccgtctttat ccgtattgaa agatattttg ccccgagcgc
     1261 aatggctgtc ttatactttg gcgggcggcg acgattacga gctggtgttt accgcgccgg
     1321 aaagttgccg cagccgcgtg cttgatgcgg cagaacggtg cggcgtgccg gtaacgcgca
     1381 tcggcaaaat caacggagga tgccgtctga aggttttaga tgccgacggc agggaattgg
     1441 aactacattc tttaggattc gatcattttg gctgatttta aacctgactt tgcgtggctg
     1501 ttgaaacgac cgttgtgttt tttggctttt ggtttcggca gcgggctggc tccgttcgcg
     1561 ccgggtacat ttggcacttt ggcggcactg cctttggcat ttgtgctgat tttgctcggc
     1621 atagacgggc tgctgctggc ttttttgtgt atcgtgctgt ttatgtgggg catacgcatt
     1681 tgcgcttatg cggaacgtga aacgggtgtc agcgaccatg gtgggattgt ttgggacgag
     1741 attgtcgcca tgctgtttgt gctggcgttt gtgccgttca ggtggacgtg gtggctggcg
     1801 gcatttgtcc tattccgtct gtttgacgcg ctcaaaccgt ctcccgtcgg ttggtttgac
     1861 aagaatctgc acggcggttt gggcattatg gcggacgata tggcggctgc ggtgatgact
     1921 ttgattgtct tgaggattgc aatgctgttt taaacggtgc tgccttgtaa aaatgccgcc
     1981 tgaaagcctt tcagacggca ttgtttcgga ggttaacgcg ttaccggttt gtatttgatg
     2041 cgtttcggtt tcgcgccttc ttcgccgagt cggcgtttct tgtcggcttc gtattcctga
     2101 tagttgccgt cgaagaacac ccatttggag tcgccttcgc aagccaagat atgcgtagca
     2161 atacggtcga ggaaccaacg gtcgtgcgaa atcaccatca cgctaccggc aaattccagc
     2221 aatgcgtctt ccagcgcgcg cagggtttct acgtcaaggt cattggacgg ttcgtccaac
     2281 agcaatacat tgccgccgcc caacaaggtt tttgccaagt gcaaacgtcc gcgttcgccg
     2341 ccggaaagct gccccgtgat tttgctttgg tcgctgcctt tgaaattgaa gcgtcccaaa
     2401 tattggcggg cggggatttc aaactgcccg acctgtaaaa tatcgcgacc ttcggcaatg
     2461 ttgtcgaaca cggttttgtc gttttgcaaa ccttcgcggc tttggtcaat caagctcatt
     2521 ttcacggttt gcccgatttt cacttcaccg gaatcgggct gctctttgcc cgcaatcatt
     2581 ttaaacagtg tcgatttacc cgcgccgttc ggaccgatga tgccgacaat cgcgcccgca
     2641 ggcactttga agctcaaatc gtcaatcagc actttgtcgc cgaacgattt ggaaacattc
     2701 acaaattcaa tcacttcgtt acccaaacgc tcggcgacgg gaatgaagat ttcctgcgtt
     2761 tcattgcgtt tttggtattc gtagttgctc atttcttcaa aacgagccaa acgcgctttg
     2821 gacttggctt ggcggccttt ggcattttgg cgcacccatt ccaattcctg cttcatcgcc
     2881 ttcacgcgcg cggcttcgga ttttgcctcg ttttccaagc gtttttcttt ctgctccagc
     2941 caagacgagt aattgccttt ccacggaata ccatggccgc ggtcgagttc caaaatccat
     3001 tcggcggcgt tgtcgaggaa gtagcggtcg tgtgttaccg caacgactgt accggggaag
     3061 cgcacgagaa attgctccag ccactcgaca gattccgcgt ccaagtggtt ggtcggctcg
     3121 tccagcaaaa gcatatcggg tttgctcaac aagagtttgc acaaggcaac gcggcgtttt
     3181 tcaccgccgg acaaattatc gattttggca tcccattccg gcaggcgcag cgcgtcggcg
     3241 gcgatttcca attcgtgttc cgcaccgccg cccgtggacg aacctgccgc aataatcgct
     3301 tccaagcggc cctgctcttc tgccaacgcg tcaaaatccg catcaggatt ggcgtactcg
     3361 gcatacactt cttccaaacg tttctgcgcg gcagccactt cgcccaaacc gctttccact
     3421 tcctcgcgca cggttttttc aggatcaagc tcaggctctt gcggcaggta gccgatttta
     3481 atgccgccca tcggcacggc ttcgccctca aattccttat ccacacccgc cataatccgc
     3541 agcacggtgg acttgcccgc gccgttcaaa ccgagcaggc cgattttcgc gccggggaag
     3601 aaagaaaggg aaatatcttt aatgatggtt ttctgcggcg gcacaacctt gctcacgcgc
     3661 agcatagaat agacgtattg ttgggacatg gttttctcgt tttcatcaaa caaatttcag
     3721 acggccattt taaccgataa tttgatttaa gccagtttat ccgcgaaccg gtattgccaa
     3781 aatcgggcag gattcataaa atccgcttat ccctttgaaa ttatatagac aaaaaaataa
     3841 taatgatagg ggatcgccgc cccggcaacc atttcggatt ttccaaag
//
LOCUS       DAWFJW010000144         3861 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFJW010000144 DAWFJW010000000
VERSION     DAWFJW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.144"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(1..366)
                     /locus_tag="WGR19_001912"
                     /pseudo
     CDS             complement(1..366)
                     /locus_tag="WGR19_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            627..2099
                     /gene="pyk"
                     /locus_tag="WGR19_001913"
     CDS             627..2099
                     /gene="pyk"
                     /locus_tag="WGR19_001913"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0799377.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2409..3803
                     /locus_tag="WGR19_001914"
     CDS             2409..3803
                     /locus_tag="WGR19_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0799378.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELHKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAAAKAMWSTML
                     AANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKEK
                     TIGMGKKSDRTTITPNWRNTYKVGLGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTTYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT         1011 a         1145 c          947 g          758 t
ORIGIN      
        1 ctgtcaggtt tcacttgttc acggataata ggaaataaag tagcggtttg aattcggtac
       61 tgtaaccgta taaaccttac catttcgctt caaaagaccg aatacggcga ctttaccggc
      121 agcaccgcga ccgcgtttgc ctttgcgttg tccgccaaaa taactttcat ctgcttctac
      181 ttcgccatca aacatttcca aatgcggact gttttgataa ataagtaatc gtaaccgatg
      241 aaaataatag gctgcggtat ttttattaac gcctactaac tctgctgccg ttcttgcagt
      301 tacacctgcg acaaacagtt caatgagttt attttgttta taccggctta gacgactttt
      361 tctcataggg ataattctaa cttaatttga atttccctag ttatctagga cagccccaaa
      421 aataaattta gcccaagtca taaaaccgtg taattttgca ccaaaaaagg cggtttgcag
      481 gtaaaatatc aaactattaa tttaaatcat atttttacag aatattccgc cgcgttcatc
      541 aaatggacat caaaccggtt ccaaatttct gtaattttgt aacaaaatac cgcaaaacac
      601 ccgattgaga ccaaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca
      661 gccacaacac caaaatcgtc gccacccttg ggccgggcag caacaacgta gaactgttgg
      721 aagatatgat ccgcgtcggc ggcctgaacg tcgtccgctt caacttcagc cacggcacgc
      781 ccgaattcca tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc gccggacagg
      841 aaatcgccat cattgccgac ctgcaagggc cgaaaatccg cgtgggcaaa atcgccggcg
      901 gcggcattga attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc gaaggcgaag
      961 gcacgcgcga ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt gccgcaggcg
     1021 acgtattgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca
     1081 ggattatcac aagggtggaa aacagccacg tcctgaaaag caacaagggc atcaacaaac
     1141 gcggcggcgg tctgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg
     1201 cgattgccat cggttgcgac tacctcgcca tcagctttgt gaaatccgcc gaagatttgc
     1261 atatcgcgcg cgccaaagtc gaagaagaaa tgaagggcag cacggccgtg cgccccggtt
     1321 tggtttccaa aatcgaacgc gtggaagcga ttgaaaactt ggacgaaatc atccttgccg
     1381 gcgacggcat tatggttgcg cgcggcgact tggcggtcga agtcggacac gccgccgtcc
     1441 ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg
     1501 cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg
     1561 atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg
     1621 tcggcgcgta tccgtttgaa accgtcagcc aaatggcaat tatctgcgcg gctgcggaaa
     1681 aagagcagga ttcgctcaac ggcgttgccg aacaggcgga gtatcccgaa gcggtcagca
     1741 ccaacctggc ggttgccggc ggcgcggtca gcgtggcgcg cgcggttcac gccaaagcca
     1801 tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aacatcaccc
     1861 tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg
     1921 gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgtta aacgaagtcg
     1981 aaaccatgct ggtggaacac aacatcctgc attccggcga ccaatacatc attaccagcg
     2041 gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat
     2101 ccgccctgag tggaaaatgc cgtctgaagt cgatgcccga ggcttcagac ggcatttttt
     2161 gcggcggcgc ggcggttcga gcaaactctg aaaaaattgt gcaaatccgg caacatcgga
     2221 taaaatcgag tacctatact aaagcgaaac aaggcatttc cgactgcctt ttttatagtg
     2281 gattaaattt aaaccagtac ggcgttgcct tgccgtacta tctgtactgt ctgcggcttc
     2341 gtcgccttgt cctgattttt gttaatccac tatatttatc caccgtccgc ctttttacgg
     2401 aaaccgaaat gaccccttcc gcactgaaaa aaaccgtcct actgctcggc actgcctttg
     2461 ccgccgcatc cgtccacgca tccggctacc acttcggcac acagtcggtc aacgcgcaaa
     2521 gcacggcaaa tgccgccgcc gcagaagccg ccgacgcatc gaccatcttc tacaaccctg
     2581 ccggcctgac caaactcgac agcagccaga tttccgtcaa cgccaacatc gtgctgccca
     2641 gcattcatta tgaggcggat tccgccaccg actttaccgg gcttcccgtc caaggttcga
     2701 aaagcggcaa aatcaccaaa accacggtcg cgccccacat ctacggcgca tacaaagtca
     2761 acgacaatct gaccgtgggc ttgggcgtgt acgtcccctt cggctccgcc accgaatacg
     2821 aaaaagattc cgtgttgcgc cacaacatca acaaactcgg tctgaccagc atcgccgtcg
     2881 aacctgtcgc cgcgtggaaa ctcaacgacc gccattcctt cggcgcaggc atcatcgccc
     2941 aacatacttc cgccgagctg cacaaatatg ccgactgggg gattatggaa aaagcgaaag
     3001 cactaaaaga aacacccccc aatccaacta aagccgccca aatcaaagcc gacggacacg
     3061 ccgatgtcaa aggcagcgat tggggcttcg gctaccaact ggcgtggatg tgggacatca
     3121 acgaccgtgc gcgcgtgggc gtgaactacc gttccaaagt ctcacacacg ctcaaaggcg
     3181 atgccgaatg ggcggcagac gacgcggcgg cgaaagcaat gtggagtacg atgcttgcag
     3241 caaacggcta cacggcgaat gaaaaagccc gcgtcaaaat cgtaacgcct gagtctttgt
     3301 ccgtacacgg catgtacaaa gtgtccgaca aagccgacct gttcggcgac gtaacttgga
     3361 cgcgccacag ccgcttcgat aaggcggaac tggtttttga aaaagaaaaa accatcggta
     3421 tgggcaaaaa atccgaccgc accaccatca cccccaactg gcgcaacacc tacaaagtcg
     3481 gcttgggcgg ttcttatcaa atcagcgaac cgctgcaact gcgcgccggc atcgcttttg
     3541 acaaatcgcc cgtccgcaac gccgactacc gcatgaacag cctgcccgac ggcaaccgca
     3601 tctggttctc cgccggtatg aaatatcata tcggcaaaaa ccacgtcgtc gatgccgcct
     3661 acacccacat ccacatcaac gacaccacct accgcacggc gaaggcaagc ggcaacgatg
     3721 tggacagcaa aggcgcgtct tccgcacgtt tcaaaaacca cgccgacatc atcggcctgc
     3781 aatacaccta caaattcaaa taaacggcct gccgtttgaa tgtaacaatg ccgtctgaaa
     3841 caggtattgt tttaggctcg a
//
LOCUS       DAWFJW010000145         3822 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFJW010000145 DAWFJW010000000
VERSION     DAWFJW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.145"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            46..204
                     /locus_tag="WGR19_001915"
     CDS             46..204
                     /locus_tag="WGR19_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799379.1"
                     /translation="MPSEHAFGRRFYNKNTSWRLVLCLSKFLCRAYLKISDGIGLCCL
                     KSVFRFSL"
     gene            286..1407
                     /gene="dnaJ"
                     /locus_tag="WGR19_001916"
     CDS             286..1407
                     /gene="dnaJ"
                     /locus_tag="WGR19_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0799380.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1602..3620
                     /locus_tag="WGR19_001917"
     CDS             1602..3620
                     /locus_tag="WGR19_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0799381.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSELPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3664..>3822)
                     /locus_tag="WGR19_001918"
     CDS             complement(3664..>3822)
                     /locus_tag="WGR19_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEZ0799382.1"
                     /translation="DYYKPEAEQVLIWNDPAIGIDWPLQTEPLLSPKDLAGKAWAQAE
                     KLRLTLSR"
BASE COUNT          809 a          853 c         1144 g         1016 t
ORIGIN      
        1 gaaaaagacc tgcgctatat cggggaatga atcgacgcaa aggaaatgcc gtctgaacat
       61 gcgttcgggc ggcgttttta taacaaaaac acttcatggc ggttggtttt atgcctgtct
      121 aagtttttgt gtcgtgcata cctgaagatt tcagacggca tcggtttatg ctgtctgaaa
      181 agtgtattcc gtttcagttt gtaagctatg gcagtctgtt tgtcttgtgt tttgcgtaat
      241 tgcccttatt ttaagccgtg attttatttt gaattagatg aaaaaatgag taatcaagat
      301 ttttatgcga cgctgggtgt ggcaagaaca gctaccgatg atgagattaa aaaagcctac
      361 cggaaattgg cgatgaaata tcatcccgac cgcaatcctg acaataaaga ggcggaagag
      421 aagtttaaag aagtacaaaa ggcgtatgaa actttgtccg acaaggaaaa gcgcgctatg
      481 tacgaccagt atggtcatgc ggcgtttgaa ggcggcggac aggggggctt cggagggttt
      541 ggcggatttg gcggtgcgca gggttttgac tttggggata ttttcagcca aatgtttgga
      601 ggcggttcgg ggcgcgccca gcctgattat cagggtgagg acgttcaagt cggtatcgaa
      661 atcacgcttg aagaagccgc aaaaggtgtg aagaaacgca tcaatattcc gacttatgaa
      721 gcgtgtgatg tctgtaacgg cagcggcgcg aaaccgggga catccccgga aacctgcccg
      781 acttgcaaag gttcgggtac ggtgcacatc cagcaggcga ttttccgtat gcagcagact
      841 tgtccgacct gccacggtgc gggcaagcac attaaagaac cttgcgtcaa atgccgtggc
      901 gcggggcgga ataaggcggt caagacggtg gaagtcaata ttcccgccgg tatcgatgac
      961 gggcagcgta tccgtttgag cggcgaaggc ggaccgggta tgcacggtgc gcctgccggc
     1021 gacctgtatg taaccgtccg cattcgggcg cataagattt tccaacgcga cggtctggac
     1081 ttgcattgcg aactgccgat cagttttgcc acggctgctt tgggcgggga gttggaagtg
     1141 ccgaccttgg acggaaaggt caagctcacc gtccccaaag aaacccaaac cggcaggagg
     1201 atgcgtgtga aaggtaaggg tgtcaaatcg ttgcgcagca gcgcgaccgg cgatttgtac
     1261 tgccatattg ttgtcgaaac gcctgtcaat ttgaccgacc gtcaaaaaga gcttttggaa
     1321 gagtttgagc ggatttctac cggcttggaa aaccaaacac cgcgcaagaa atcgttttta
     1381 gacaagctgc gcgatttgtt tgattgattt taaggttcgg aaacaagcag ccgtatcggg
     1441 gaatctccct gatacggctg ttttgtttgt ttaaaaatag tttttatttt caatggggta
     1501 tggggcaggg tgggataact gtttttaact gttcttttta aaacttgaca tcatggcgcg
     1561 atgccaacaa tatgtgaacg tctgttgtca aaggaagaat aatgaataaa tctttatccg
     1621 ggtcggtaga agaataccgc gaactgacgc tccgaggcat gatactcggt gcattgatca
     1681 ctgtaatttt tactgcgtcc aacgtttacc tcggtttgaa agtcgggctg acctttgcct
     1741 cgtcgattcc ggcggcggta atttcgatgg cggttttaaa gtttttcaaa ggcagcaata
     1801 ttttggaaaa caacatggtg cagacccaag cctcggctgc gggtacgctt tcgaccatca
     1861 tcttcgtcct gcccggtttg ctgatggcgg gctactggag cggtttcccg ttctggcaga
     1921 cgacgctttt atgtattgcc ggcgggattt tgggggtgat ttttaccatt ccgctgcgtt
     1981 acgcgatggt ggtaaaaagt gagctgcctt acccggaagg tgtggcggct gctgaaattt
     2041 tgaaagtggg cggtcatgaa gaaggggata accgtcaggg aggcagcggc atcaaagagc
     2101 tggcggccgg cggtgcgttg gcgggattga tgagcttttg cgccggaggt ctgcgcgtga
     2161 ttgccgacag cgcgagttat tggtttaaaa gcggtacggc gattttccaa ctgccgatgg
     2221 gcttttcact ggcattgttg ggcgcgggct atttggtcgg actgacgggc ggtatcgcca
     2281 tcctgttggg catttcgatt gcttggggca ttgccgtgcc gtatttctcc tcacacattc
     2341 cgcaaccttc cgatatggaa atggcggcgt ttgcgatgaa gctgtggaag gagaaagtgc
     2401 gttttatcgg tgcggggact attggcattg cggcggtttg gacgctgttg atgctgctca
     2461 agccgatggt ggaaggcatg aagatgtcgt tcaagagttt tggcggcggt gcgcccgctg
     2521 cggaacgcgc cgaacaggat ttgtcgccta aagccatgat tttttgggtg ctggcgatga
     2581 tgtttgtttt aggcgtgtcg ttttaccact ttatcggcga ttcgcacatt acgggcggca
     2641 tggcttggct tttggtggtc gtttgcacgc ttttggcttc cgtcatcggc tttttggtcg
     2701 ccgccgcctg cggttatatg gcaggtttgg tcggctcgtc ttccagcccg atttccggcg
     2761 tgggcatcgt gtccgtcgtc gttatttcac tggttttgct gctggtaggt gaatccggag
     2821 gtttgttggc ggatgaggct aaccgcaaat ttttgctggc actgactttg ttttgcggct
     2881 cggcagtaat ctgcgtggct tcgatttcca atgacaacct gcaagacttg aaaaccggct
     2941 acctgctcaa agccacgcct tggcggcagc aagtcgccct gattatcggc tgtatcgttg
     3001 gtgcgctggt tatttcgccc gtgttggaac tgctttacga agcctacggc tttaccggcg
     3061 caatgccgcg cgaaggcatg gatgcggcgc aggctttggc ggctcctcaa gcgactttga
     3121 tgacgaccat cgcgtcgggc attttcgccc acaacctcga atgggtctac atctttaccg
     3181 gcatcgcgat tggagcggta ttaatcgtcg tcgatttggt gttgaaaaaa tcatcaggcg
     3241 gcaaacttgc cctgcccgtc cttgcggtcg gtatgggtat ttatctgccg ccgtccgtca
     3301 atatgcccat cgtggcaggc gcggtgttgg cggcggtgtt gaaacacatc atcggtaaaa
     3361 aagcggaaaa ccgcgaaggc cgtctgaaaa acgccgagcg catcggaacc ttgttctccg
     3421 ccggcctgat tgtcggtgaa agcctgatcg gtgtgattat ggcgtttatt attgccttct
     3481 ccgtgaccaa cggcggctcg gatgcgccgc tcgcgttgaa tctgcaaaac tgggatgccg
     3541 ccgcttcttg gttgggtttg gcgttcttcg ttaccgggat gtttttcttt gcacagcgcg
     3601 tactgaaggc gggtaggtag gctgttagaa aaaatgccgt ctgaaacgtt cagacggcat
     3661 tttttatcgg gaaagcgtaa ggcggagctt ttcagcttgc gcccatgctt taccggcaag
     3721 atctttgggc gacagcagcg gctcggtttg aagcggccag tctatgccta ttgccgggtc
     3781 gttccatatt aaaacctgtt cggcttcagg cttgtaatag tc
//
LOCUS       DAWFJW010000146         3605 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFJW010000146 DAWFJW010000000
VERSION     DAWFJW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.146"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..414)
                     /locus_tag="WGR19_001919"
     CDS             complement(<1..414)
                     /locus_tag="WGR19_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ0799383.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
     gene            562..1014
                     /gene="dut"
                     /locus_tag="WGR19_001920"
     CDS             562..1014
                     /gene="dut"
                     /locus_tag="WGR19_001920"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0799384.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1090..2277
                     /gene="dapC"
                     /locus_tag="WGR19_001921"
     CDS             1090..2277
                     /gene="dapC"
                     /locus_tag="WGR19_001921"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0799385.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2440..3219
                     /gene="yaaA"
                     /locus_tag="WGR19_001922"
     CDS             2440..3219
                     /gene="yaaA"
                     /locus_tag="WGR19_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0799386.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRELALQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRAQLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3302..3392
                     /locus_tag="WGR19_001923"
     tRNA            3302..3392
                     /locus_tag="WGR19_001923"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3336..3338,aa:Ser,seq:tga)
     gene            3470..3559
                     /locus_tag="WGR19_001924"
     tRNA            3470..3559
                     /locus_tag="WGR19_001924"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3504..3506,aa:Ser,seq:cga)
BASE COUNT          926 a          850 c          882 g          947 t
ORIGIN      
        1 aggcatattg aatgcgggca aaccggctgc tttccgtttt tggatttcgg agaatgccat
       61 cgcccagctt tcatcacaca taaaaaacag tgcgggcacg gcttttttca gcggtatttc
      121 tttcaggtgc ggggcaagtg ccgcccccat caggatatgc cgcgaattaa tcataaaggt
      181 tacggtggcg ataagcagta tcggcagagg ttccgcccac aggttgaccg tggcaaactc
      241 ggagccgccg gcgaagttca tactggtcat caacaacatt tccagccagc tcatgccttt
      301 ttgtccgccc tgcataccga gtattaatgc ccaaggcaga agtccgataa gtatgggcga
      361 actttctttg atgccgcgta taaattcgtt acggggcgag cttatgtgtg tcatattata
      421 atattgatat gtaagaaggt cgggcattat acacagacgg agtatttcaa aacaaaacct
      481 atttgctgtg tttcagtgta aacacggctt gtgtataatc caccatcttt agacagaccg
      541 accgtatgtg ggagcaaggc aatgaatatt gaagtagaaa tgaaagtgtt ggacgagagg
      601 atggcggatt ttatccctac ctatgcgaca gcgggttctg ccggtttgga cttgcgcgcc
      661 tgtttggatg aggaagtcgt cttgcagccg ggtgaaacgt ttcttgtacc gacaggtttg
      721 gcaatttatt tggcgaatcc cgcatatgcc gccgttttgc tgccccgttc cggcttgggg
      781 cataaacacg gcattgtttt gggcaatttg gtcggtttga ttgactccga ttatcaaggg
      841 gaattgaagg tgtcgttatg gaacagaagc agcgagccgt ttactgtcaa accgttcgag
      901 cgtatcgcac agatggttat cgttccggtc gtacaggcgg gcttcaaacg tgtcgaggaa
      961 tttgtcggaa gcagccgggg tgagggcggc ttcggcagta cgggtttgca ttgaatataa
     1021 aatgccgtct gaagggcacg tcaggttcag acggcatatc ttccgatata ccgacaaccg
     1081 gagaaaatca tgaatacctt actcaaccaa ctcaaaccct atccctttgc ccgactgcgt
     1141 gaagcgatgc agggcatttc cgcccccgaa ggtctggaag ccgtcccact gcatatcggc
     1201 gaaccgaaac atccgacacc gaaagtcatt acggatgcgc tgaccgcctc attgcacgag
     1261 ttggaaaaat atccgctgac ggccggtttg cctgaactgc gtcaggcgtg tgcgaactgg
     1321 ttaaaacgcc gttatgatgg gttgacgctg aattccgaca ccgaaattct gccggttttg
     1381 ggaagtaggg aggcgttgtt ttcttttgtc caaaccgtgt tgaaccctgt ttcagacggc
     1441 ctcaaacccg caattgtcag cccgaatccc ttttatcaga tttatgaagg tgcgacactt
     1501 ttgggtggcg gtgaaatcca ttttgctaat tgccccgcgc cgtctttcaa ccccgattgg
     1561 cgcagtattt ccgaagaggt ttggaaacgc accaaactgg tgttcgtctg ctcgcccaac
     1621 aaccccagcg gcagcgtgct ggatttggat ggatggaaag aagtttttga tttacaggat
     1681 aaatatggtt tcattattgc ctcggatgaa tgctattccg aaatctattt cgacggcaac
     1741 aaacctttag gctgcctgca ggccgctgca cagttgggtc gaagcaggca aaaactgctt
     1801 atgttcacca gtttgtccaa gcgttccaac gttccgggcc tgcgttccgg ttttgtcgcc
     1861 ggcgatgccg aactgcttaa aaactttctg ctttacagaa cctatcacgg cagtgcaatg
     1921 agcattcccg tgcagcgcgc aagcattgcc gcttgggatg acgaacagca cgttatcgac
     1981 aaccgccgcc tgtatcagga aaaatttgag cgcgttattc ccattttgca acaggtattt
     2041 gatgttaaat taccagatgc ctcgttttac atttggttga aagtccccga tggcgacgat
     2101 ttggcatttg cacgcaattt atggcaaaaa gccgctatcc aagtattgcc tggacgtttt
     2161 ttggcacggg ataccgaaca gggcaatccc ggggaaggtt atgtgcgtat tgctttggtt
     2221 gccgatgtcg caacttgtgt caaagctgcg gaaaccattg tttccctata tcggtaaaga
     2281 ataaaaaata ccgtctgaac ttttgttcag acggcatttt tcaatatttt acggttgaat
     2341 ttgctataac ggtatttact tcacttttaa tctaatcggc ttgcccgcaa acacgtttaa
     2401 acttaaaatc cccgtgtttg acacaatacc gagcagatta tgttttttgt cctttcccct
     2461 gcgaaaaacc ttaacgaaaa agaccctgcc cctgtcagcg agtttaccca acccgacctg
     2521 ctggcagagt ccgaaattct aatgcagcag ctgcgcgagc ttgcgttgca acagattgcc
     2581 gaactgatgc acgtttccga caaaattgcc ctcttaaacg cgcagcgcaa tgcagaatgg
     2641 cacacgccgt ttacgccgga aaacgccaaa caggcggtct ttatgttcaa cggcgatgtt
     2701 tacgaaggta tggatgcaaa cacattggat attggacaga tacgctatct gcaaaaccat
     2761 gtccgcctgc tgtccggtct atacggtctt cttcgcccgt tagacctgat acagccctat
     2821 cgtttggaaa tggggacggc atttgccaat ttgcgcggca agaatttgta tgagttttgg
     2881 ggcgacatca ttaccaacct tttaaatgac acgcttgccc aagcaggcag caatacgctt
     2941 gtcaaccttg cctcacagga atatttcaag tccgtcaaca cgaaaacact tcgggcgcag
     3001 ctgattaccc cgatatttaa agacgaaaaa aacggtaaat ataaaatcat cagtttctat
     3061 gccaagcgtg cgcgcggatt aatggtgcgc tatgcggcag aacatcatat taccgaccct
     3121 gaaatgctga aaaattttaa ttacgaaggc tacgcattca atgacgcggc ttcaaatgaa
     3181 agcgaatggg tttttatgcg ttcggaacaa ataaagtgaa aacaataaat taagtatttt
     3241 ccgaaaaaag tgcttggcaa aatgtataaa cttcattatt attcttcttc ttcaagaaga
     3301 cggaagcgtg gcagagcggt ttaatgcaac ggtcttgaaa accgtcgagg gttgatagcc
     3361 ctccgtgagt tcgaatctca ccgcttccgc caatccttgg ggtcaaaaac caaataaaaa
     3421 tgaagtagca taaagcatcg gcatttttat ttggttttac ttcattatcg gaaacgtggc
     3481 agagaggctg aatgcagcgg actcgaaatc cgctgagggt gcaaatcctc cgtgggttcg
     3541 aatcccaccg tttccgccac aaaacaaaac cgccctgatt cggggcggtt cttttttgtt
     3601 caagt
//
LOCUS       DAWFJW010000147         3540 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFJW010000147 DAWFJW010000000
VERSION     DAWFJW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.147"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            268..3033
                     /locus_tag="WGR19_001925"
     CDS             268..3033
                     /locus_tag="WGR19_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0799387.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGNNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYNPYKNYNNQELQKYIEDHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTRDVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            3063..3305
                     /locus_tag="WGR19_001926"
     CDS             3063..3305
                     /locus_tag="WGR19_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799388.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYDSSFPRRRESRHSTLRQFIGND"
BASE COUNT          999 a          871 c          859 g          811 t
ORIGIN      
        1 ggattaataa aaatcaggac aaggcggcga gccacagaca gtacaaacag tacggaaccg
       61 attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaaag cgagacaacg
      121 ctgtactggt ttttgttaat ccactataaa tatccaattg aaatcttcag acggtatatc
      181 aaatttacac ttttttaatg tttatgccgc ctgaaaaaaa tgctagtata tttcctaatt
      241 gtctgactgt ttattgttga ggaaaatatg agatcttctt tccggttgaa gccgatttgt
      301 ttttacctta tgggtgttat gctatatcat catagttatg ccgaagatgc agggcgcgcg
      361 ggcagcgagg cgcagataca ggttttggaa gatgtgcacg tcaaggcgaa gcgcgtaccg
      421 aaagacaaaa aagtgtttac cgatgcgcgt gccgtatcga cccgtcagga tatattcaaa
      481 tccagcgaaa acctcgacaa catcgtacgc agcatccccg gtgcgtttac acagcaagat
      541 aaaagctcgg gcattgtgtc tttgaatatt cgcggcgaca gcgggttcgg gcgggtcaat
      601 acgatggtgg acggcatcac gcagaccttt tattcgactt ctaccgatgc gggcagggca
      661 ggcggttcat ctcaattcgg tgcatctgtc gacagcaatt ttattgccgg actggatgtc
      721 gtcaaaggca gcttcagcgg ctcggcaggc atcaacagcc ttgccggttc ggcgaatctg
      781 cggactttag gcgtggatga cgtcgttcag ggcaataata cctacggcct gctgctaaaa
      841 ggtctgaccg gcaacaattc aaccaaaggt aatgcgatgg cggcgatagg tgcgcgcaaa
      901 tggctggaaa gcggagcatc tgtcggtgtg ctttacgggc acagcaggcg cagcgtggcg
      961 caaaattacc gcgtgggcgg cggcgggcag cacatcggaa attttggcgc ggaatatttg
     1021 gaacggcgca agcagcgata ttttgtacaa gagggtgctt tgaaattcaa ttccgacagc
     1081 ggaaaatggg agcgggattt acaaaggcaa cagtggaaat acaatccgta taaaaattac
     1141 aacaaccaag aactacaaaa atacatcgaa gaccatgata aaagctggcg ggaaaacctg
     1201 gcgccgcaat acgacatcac ccccatcgat ccgtccagcc tgaagcagca gtcggcaggc
     1261 aatctgttta aattggaata cgacggcgta ttcaataaat acacggcgca atttcgcgat
     1321 ttaaacacca aaatcggcag ccgcaaaatc atcaaccgca attatcaatt caattacggt
     1381 ttgtctttga acccgtatac caacctcaat ctgaccgcag cctacaattc gggcaggcag
     1441 aaatatccga aagggtcgaa gtttacaggc tggggacttt taaaggattt tgaaacctac
     1501 aacaacgcga aaatcctcga cctcaacaac accgccacct tccggctgcc ccgcgaaacc
     1561 gagttgcaaa ccacgttggg cttcaattat ttccacaacg aatacggcaa aaaccgcttt
     1621 cctgaagaat tggggctgtt tttcgacggt cctgatcagg acaacgggct ttattcctat
     1681 ttggggcggt ttaagggcga taaagggctg ctgccccaaa aatcaaccat tgtccaaccg
     1741 gccggcagcc aatatttcaa cacgttctac ttcgatgccg cgctcaaaaa agacatttac
     1801 cgcttaaact acagcaccaa taccgtcggc taccgtttcg gcggcgaata tacgggctat
     1861 tacagctcgg aaggcgaatt taagcgggca ttcggagaaa actcgccgac atacaagaaa
     1921 cattgcaacc cgagctgcgg gctttatgaa cccgtattga aaaaatacgg caaaaagcgc
     1981 gccaacaacc attcggtcag cattagtgcg gacttcggcg attatttcat gccgttcgcc
     2041 agctattcgc gcacacaccg tatgcccaac atccaagaaa tgtatttttc ccaaatcggc
     2101 gactccggcg ttcacaccgc cttaaaacca gagcgcgcaa acacttggca atttggcttc
     2161 aatacctata aaaaaggatt gttaaaacaa gatgatacat taggattaaa actggtcggc
     2221 taccgcagcc gcatcgacaa ctacatccac aacgtttacg ggaaatggtg ggatttgaac
     2281 gggaatattc cgagctgggt cagcagcacc gggcttgcct acaccatcca acaccgcaat
     2341 ttcaaagaca aagtacacaa acacggtttt gagttggagc tgaattacga ttatgggcgt
     2401 tttttcacca acctttctta cgcctatcaa aaaagcacgc aaccgaccaa cttcagcgat
     2461 gcgagcgaat cgcccaacaa tgcgtccaaa gaagaccaac tcaaacaagg ttatgggttg
     2521 agcagggttt ccgccctgcc gcgagattac ggacgtttgg aagtcggtac gcgctggttg
     2581 ggcaacaaac tgactttggg cggcgcgatg cgctatttcg gcaagagcat ccgcgcgacg
     2641 gctgaagaac gctatatcga cggcaccaac gggggaaata cccgcgatgt ccggcaactg
     2701 ggcaagcgtt ccatcaaaca aaccgaaacc cttgcccgcc agcctttgat ttttgatttt
     2761 tacgccgctt acgagccgaa gaaaaacctt attttccgcg ccgaagtcaa aaatctgttc
     2821 gacaggcgtt atatcgatcc gctcgatgcg ggcaatgatg cggcaacgca gcgttattac
     2881 agctcgttcg acccgaaaga caaggacgaa gacgtaacgt gtaatgctga taaaacgttg
     2941 tgcaacggca aatacggcgg cacaagcaaa agcgtattga ccaattttgc acgcggacgc
     3001 acctttttga tgacgatgag ctacaagttt taaaggcagc ccgcgttttg tggaaaaccg
     3061 caatgccgtc tgaaagccct tcagacggca tttgtttccc caaacgcatc atcctgccgc
     3121 aagcctatgc caatccgttt tatcgcatcg gcaactcaaa gaaaaatcca tttcattccc
     3181 acgcagggaa gccggttttt gatttcggtt atttttggtt gtttcgggta atttatgatt
     3241 cgtcattccc gcgcaggcgg gaatctagac attcaacgct aaggcaattt atcggaaatg
     3301 actgaaactc aaaaaactgg attcccactt tcgtgggaat gacgcggtgc aggtttccgt
     3361 atggatggat tcgtcattcc cgcgcaggcg ggaatctaga cattcaacgc taaggcaatt
     3421 tatcggaaat gactgaaact caaaaaactg gattcccact ttcgtgggaa tgacgtagta
     3481 caggtttccg tatggatgga ttcgtcattc ccgcgcaggc gggaatctag accgttcggt
//
LOCUS       DAWFJW010000148         3444 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFJW010000148 DAWFJW010000000
VERSION     DAWFJW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.148"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            36..704
                     /locus_tag="WGR19_001927"
     CDS             36..704
                     /locus_tag="WGR19_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0799389.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYST
                     NQDLTNTSRFVYSDGYIQIVLTDAKAVSADEKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            734..1354
                     /gene="pilV"
                     /locus_tag="WGR19_001928"
     CDS             734..1354
                     /gene="pilV"
                     /locus_tag="WGR19_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0799390.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNNTLSHTLSAVDGEFA
                     IDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTFSGNAFSSNCDN
                     KANGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            1351..2292
                     /locus_tag="WGR19_001929"
     CDS             1351..2292
                     /locus_tag="WGR19_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0799391.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKDVVSNGAQKNPL
                     FSLKRNSTNKLIPITESSNINYQNFFQVGSALIFQYGIDDVNASTATTVVSSCAAISK
                     PGKQIPTLEDAKKELKIPDQDKEQNGNIARQRHEVNAYAVGGIAGEEGLFRFQLNEKG
                     EWGNPQLLVKKIRHMKVRYIYVSDCPEDDDAGKEETFRYTDKFNSAQDAVTPAGVEVL
                     LSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2271..2867
                     /locus_tag="WGR19_001930"
     CDS             2271..2867
                     /locus_tag="WGR19_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0799392.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NNTGNEEAFDNIVVQGKPTVEAVKRSCPANSTNLCIDKKGMEYKKGTRSVSKMPRYII
                     EYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2872..3369
                     /locus_tag="WGR19_001931"
     CDS             2872..3369
                     /locus_tag="WGR19_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0799393.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQIILKNPQDSNDILNIKLTRFVSGYKMNPKIAEKYSVSGEFVDAPKSRA
                     SRALKSRAYRLVGVPKAGTGYTLSVWMNSVGDGYKCRDKASAEAYSETLSSDVGCEAF
                     SNRKK"
BASE COUNT          998 a          680 c          876 g          890 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcatgcg gagcggattg
      181 ccaacctttt gcgtttctcc aggggagaag ccgtccggct caatctccct gtctatatct
      241 gtcctgttca agttaaaaaa gacggtacgg tcaacaataa atgtgactcc ggcaagaagg
      301 ggcagggaat gttggctttc ggcgacaaaa acggcaataa gggatatgac aatgatacgg
      361 cggatgttct tctccgcagt gtggtattga atgatgatat caatgataag cggattaatt
      421 atgccttcaa ccatatcgct tttggtcaga ctcagccgac caccgaccgt gtagtttgga
      481 cattcaatca aaacgggacg ttcggttatt ctaccaatca ggatctcacg aatacttcca
      541 gatttgttta ctccgacggt tatatccaaa tcgtgttgac agatgcgaag gcggtttctg
      601 ccgatgaaaa gaaattccgt tcggcggtgg ttttgattaa cagcagcggc agggttgagg
      661 tttgtcctag aaacgatagg cgtaccgtat gccaatataa atgattgttt tagtcggttt
      721 gggaaataaa aaaatgaaga ataatgattg cttccgcctg aaaaaccccc agtccggtat
      781 ggcgctgata gaagtcttgg tcgctatgct cgttctgacc atcggtattt tggcactatt
      841 gtctgttcag ttgcggacag tcgcttccgt cagggaggca gagacgcaaa ccatcgtcag
      901 ccaaatcacg caaaacctga tggagggaat gttgatgaat ccgaccattg attcggacag
      961 caacaagaaa aactataatc tttacatggg aaacaataca ctatcgcata cactatcagc
     1021 tgtggatggt gagtttgcga ttgatgccat aaaaactaag acacagttgg cagaggcaca
     1081 attgaagaga tttagttatg agctgaaaaa tgccttgccg gatgcggcag ccatccatta
     1141 cgccgtctgc aaggattcgt cgggtaacgc gccgacattc tccggcaatg ctttttcttc
     1201 aaattgcgac aataaggcaa acggggatac tttaattaaa gtattgtggg taaatgattc
     1261 ggcaggggat tcggatattt cccgtacgaa tcttgaggtg agcggcgaca atatcgtata
     1321 tacctatcag gcaagggtcg gaggtcggga atgagacgta aaatgctaaa cgtaccaaaa
     1381 ggcagttatg atggtatgaa gggttttacc attattgaat ttctggttgc aggtctgctc
     1441 agtatgattg tcctgatggc ggtcggatcg agttacttca cgtcccggaa attaaatgat
     1501 gcggcaaacg agcgtcttgc tattcaacag gatttgcgga atgcggcaac attaattgtc
     1561 cgcgatgcaa gaatggcggg gagcttcggt tgtttcaata tgtctgagca taccgaaaag
     1621 gatgttgttt ccaatggggc gcaaaaaaac cctctttttt ccttaaaaag gaacagtaca
     1681 aataaactta tccccataac ggaatcttca aatatcaatt atcagaattt tttccaggtt
     1741 ggtagcgcat tgatttttca atacggaatc gatgatgtta atgcaagcac cgcgactacc
     1801 gtcgtcagca gctgtgccgc aatatcgaaa ccgggcaagc aaatccctac tttggaagat
     1861 gcaaaaaaag aattgaagat tccggatcag gataaggagc aaaatggcaa tatagcgcgt
     1921 caaaggcatg aggtcaatgc ctatgcggtc ggcgggattg ccggtgagga aggtttgttc
     1981 cgcttccaat tgaatgaaaa gggcgagtgg ggtaatcctc agttgctcgt gaaaaagatt
     2041 agacatatga aagtgcggta tatctatgtt tccgactgtc ctgaagatga cgatgccggc
     2101 aaagaggaaa cattcagata tacggataaa ttcaacagcg cccaagatgc tgttacgcct
     2161 gccggggtgg aggttttatt gagtagcggt actgatacca agattgccgc ttcttcagac
     2221 aatcatattt atgcttaccg tatcgatgcg acaatacgcg ggggaaatgt atgcgcaaac
     2281 agaacacttt gacgggaatc ccgacttctg acggacagag ggggttcgcg ctgtttatcg
     2341 tactgatggt gatgatcgtc gtggcttttt tggttgtaac tgccgcgcag tcttacaata
     2401 ccgagcagcg gatcagtgcc aacgaatcag acaggaaatt ggctttgtct ttagccgagg
     2461 cggctttgcg ggaaggcgaa cttcaggttt tggatttgga atatgatacg gacagtaagg
     2521 ttacatttag cgaaaactgt gaaaaaggtc tgtgtaccgc agtgaatgtg cggacaaata
     2581 atactggtaa tgaagaggct tttgacaata tcgtggtgca aggcaagccc accgttgagg
     2641 cggtgaagcg ttcttgccct gcaaattcta ccaacctgtg cattgacaag aaagggatgg
     2701 aatataagaa aggcacgaga agcgtcagca aaatgccacg ttatattatc gaatatttgg
     2761 gcgtgaagaa cggagaaaat gtttatcggg ttactgccaa ggcttggggt aagaatgcca
     2821 ataccgtggt cgtccttcaa tcttatgtaa gcaataatga tgagtaataa aatggaacaa
     2881 aaagggttta cattgattga gatgatgata gtcgtcgcga tactcggcat catcagcgtc
     2941 attgccatac cttcttatca aagttatatt gaaaaaggct atcagtccca gctttatacg
     3001 gagatggtcg gtatcaacaa tactgtcaaa cagattattt tgaaaaatcc acaggatagt
     3061 aatgatatcc tcaatataaa actgacaagg tttgtctcag gctataagat gaatccgaaa
     3121 attgccgaaa aatatagtgt ttcgggggaa tttgtcgatg cgccaaaatc aagggcaagc
     3181 agggcgttaa aatcaagggc atacaggttg gtcggcgttc cgaaggcggg gacgggttat
     3241 actctgtcgg tatggatgaa cagcgtgggc gacggataca aatgccgtga taaggcttct
     3301 gccgaagcct attcggagac cttgtcctca gatgtcggct gtgaagcctt ctctaatcgt
     3361 aaaaaatagg gctgttttgc cgatgccgtc tgaaaatcaa tgttcagacg gcatttttat
     3421 gggtatagtg gattaacaaa aatc
//
LOCUS       DAWFJW010000149         3369 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFJW010000149 DAWFJW010000000
VERSION     DAWFJW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.149"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..231
                     /locus_tag="WGR19_001932"
     CDS             <1..231
                     /locus_tag="WGR19_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0799394.1"
                     /translation="VTAANGKTKKIDTKHLPSTCRDAASAICTRTPRADFKHFQKISR
                     YRVLPESRHMAEKLRHSRAGGNLGLSVRKLIG"
     gene            277..726
                     /locus_tag="WGR19_001933"
     CDS             277..726
                     /locus_tag="WGR19_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0799395.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQKVEVAKGVITAQMASSNVNNE
                     IKGKKLSLWAKRQNGSVKWFCGQPVQRAAATGAKADDVTAATDNNGINTKHLPSTCRD
                     AASAICTRTPRADFKHFQKISRYRVLPESRHMAEKLRHSRKSGNLVR"
     gene            981..1439
                     /locus_tag="WGR19_001934"
     CDS             981..1439
                     /locus_tag="WGR19_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0799396.1"
                     /translation="MDSRLSGNDESILLVEGQKSAVTEYYLNHGEWLCDNSDAGVASS
                     TDIKGKYVAGVKVEKGVITATMASSNVNNEIKDKKLSLWAKRQAGSVKWFCGQPVTRD
                     NAKATADDAVKADTAANDKKIDTKHLPSTCRDDSSVVCIETPPTAFNKNT"
     gene            1574..1966
                     /locus_tag="WGR19_001935"
                     /pseudo
     CDS             1574..1966
                     /locus_tag="WGR19_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            2006..2458
                     /locus_tag="WGR19_001936"
     CDS             2006..2458
                     /locus_tag="WGR19_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0799397.1"
                     /translation="MDSRLRGNDEAILLAEGQKSAVTEYYLNHGEWPGGNSSAGVASS
                     STIKGKYVEKVEVAKGVITATMLSSGVNKEIQGKKLSLWAKRQDGSVKWFCGQPVTRT
                     GTTTTNDEVKAATGTTANIDTKHLPSTCRDAASAVCIETPPTAFYKNT"
     gene            3230..>3369
                     /gene="pilE"
                     /locus_tag="WGR19_001937"
     CDS             3230..>3369
                     /gene="pilE"
                     /locus_tag="WGR19_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ0799398.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLA"
BASE COUNT         1025 a          979 c          703 g          662 t
ORIGIN      
        1 gtcaccgccg ccaacggcaa gaccaagaag atcgacacca agcacctgcc gtcaacctgc
       61 cgcgatgcgg catctgccat ttgcacaaga acaccacgcg ccgacttcaa acacttccaa
      121 aaaatcagcc gttaccgagt attgcccgaa tcacggcaca tggccgaaaa acttcgtcat
      181 tcccgcgcag gcgggaatct aggtttgtcg gtgcggaaac ttatcgggta aaaaggtttc
      241 tccggccctg agttctagat tcccactttc gtgggaatga cgggatttaa tgatgccgcc
      301 ggcgtggcat cctccccctc cgacatcaaa ggcaaatatg ttcagaaagt tgaagtcgca
      361 aaaggcgtca ttaccgccca aatggcttca agcaacgtaa acaatgaaat caaaggcaaa
      421 aaactctccc tgtgggccaa gcgtcaaaac ggttcggtaa aatggttctg cggacagccg
      481 gttcagcgcg ccgccgccac cggcgccaaa gccgacgacg tcaccgccgc caccgacaac
      541 aacggaatca acaccaagca cctgccgtca acctgccgcg atgcggcatc tgccatttgc
      601 acaagaacac cacgcgccga tttcaaacat tttcaaaaaa tcagccgtta ccgagtattg
      661 cccgaatcac ggcacatggc cgaaaaactt cgtcattccc gcaaaagcgg gaatctagtt
      721 cgttgagttt cagttattta gaataaattt tgaaacttta atcccgtcat tcccacgaaa
      781 gtgggaatcc agaacgcaaa atctaaagaa accgttttac ccgataagtt tccgtaccga
      841 cgaggctgga ttcccgcctg cgcgggaatg acgaagctat ccatacggaa atctacatcc
      901 cgtcattccc acgaaagtgg gaatccagaa cgcaaaatct aaagaaaccg ctttcccgat
      961 aagtttccgt accgacaagg ctggattccc gcctgagcgg gaatgacgaa tccatccttt
     1021 tggtcgaagg tcaaaaatca gccgtcaccg agtattacct gaatcacggc gaatggctct
     1081 gcgacaacag tgatgccggc gtggcatcct ccaccgacat caaaggcaaa tatgttgcag
     1141 gggttaaagt tgaaaaaggc gtcattaccg ccacaatggc ttcaagcaac gtaaacaacg
     1201 aaatcaaaga caaaaaactc tccctgtggg ccaagcgtca agccggttcg gtaaaatggt
     1261 tctgcggaca gccggttacg cgcgacaacg ccaaagccac cgccgacgac gccgtcaaag
     1321 ccgacaccgc cgccaacgac aaaaagatcg acaccaagca cctgccgtca acctgccgcg
     1381 atgattcatc tgtcgtttgc atagaaacac cacctacggc tttcaataaa aatacctaaa
     1441 ccgtcattcc cacgaaccta catcccgtca ttcccacgaa agtgggaatc cagaacgcaa
     1501 aatctaaaga aaccgtttta cccgataagt ttccgtaccg acaaggctgg attcccgcct
     1561 gcgcgggaat gacgaagcta tccttttggc cgaaggtcaa aaatcagccg tcacagagta
     1621 ttacctgaat cacggcatat ggcccggcga caacagttct gccggcgtgg caacctcctc
     1681 taaaatcaaa ggaaaatatg ttgagaaagt tgaagtcgca aacggcgtcg ttaccgccac
     1741 aatgctttca agcggcgtaa acaaagaaat ccaaggcaaa aaactctccc tgtgggccaa
     1801 gcgtcaagac ggttcggtaa aatggttctg cggacagccg gttacgcgcg acgccgtcga
     1861 caccgccaac gacgacgttg ccgccgacac caccggcacc gacaagaaga tcgacaccaa
     1921 gcacctgccg tcaacagcac cgacaagaaa atcgacacca aactagagaa acctttttac
     1981 ccgataagtt tccgtaccga cagacctgga ttcccgcctg cgcgggaatg acgaagccat
     2041 tcttttggct gaaggtcaaa aatcagccgt cacagagtat tacctgaatc acggcgaatg
     2101 gcccggcggc aacagttctg ccggcgtggc atcttcttca acaatcaaag gcaaatatgt
     2161 tgagaaagtt gaagtcgcaa aaggcgtcat taccgccaca atgctttcaa gcggcgtaaa
     2221 caaagaaatc caaggcaaaa aactctccct gtgggccaag cgtcaagacg gttcggtaaa
     2281 atggttctgc ggacagccgg ttacgcgcac cggcaccacc accaccaacg acgaagtcaa
     2341 agccgccacc ggcaccaccg ccaacatcga caccaagcac ctgccgtcaa cctgccgcga
     2401 cgcagcatct gccgtttgca tagaaacacc acctacggct ttctataaaa atacctaaac
     2461 cgtcattccc acgaacctac atcccgtcat tcccacgaaa gcgggaatct aggacgtaat
     2521 atctaaagaa accgttttcc cgataagttt ccgtaccgac agacctggat tcccgcctgc
     2581 gcgggaatga cgaatccatc catacggaaa tctacatccc gtcattccca cgaaagtggg
     2641 aatctagaat cctggacttt cagataatct ttgaatattg ctgttgttct aaggtctaga
     2701 ttcccgcctg cgcgggaatg acgaagccat ccgcacggaa acctgccgcg atgattcaac
     2761 tgccgtttgc acaggaacac cacgcgccga tttcaatcac tttccaagaa aacggaaatt
     2821 tttaaaaatt aaatttaaaa tttcccaccc aacccaccct atgctaccgc gcaaattcaa
     2881 aaatctcaaa ttccgaccca atcaacacac ccgatgcccc atgccaatag agatacccca
     2941 cgccaataaa aaagtgccga aaatcggcac taaaactgac aattttcaac actgatgaca
     3001 aaaaacggta accccctgct tcctcaaacc acacccaaac gaaagaaaat gtaaaataaa
     3061 aacaaccaca taaagataaa cgcataaatt ttcacctcaa aacacaaact tgataccaat
     3121 cttgctttat aatacacagg tatcgcaaca aaataccgat ggtcaaatac attgcataat
     3181 gccgatggcg taagcttgag gcatttcctt tccaattagg agtaatttta tgaacaccct
     3241 tcaaaaaggt tttaccctta tcgagctgat gattgtgatt gccatcgtcg gcattttggc
     3301 ggcagtcgcc cttcctgctt atcaagacta cacagcccgc gcacaagttt ccgaagccat
     3361 tcttttggc
//
LOCUS       DAWFJW010000150         3321 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFJW010000150 DAWFJW010000000
VERSION     DAWFJW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.150"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..330)
                     /locus_tag="WGR19_001938"
     CDS             complement(<1..330)
                     /locus_tag="WGR19_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0799399.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVDEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
     gene            complement(311..592)
                     /locus_tag="WGR19_001939"
     CDS             complement(311..592)
                     /locus_tag="WGR19_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0799400.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(601..1395)
                     /locus_tag="WGR19_001940"
     CDS             complement(601..1395)
                     /locus_tag="WGR19_001940"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0799401.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1779..3113
                     /gene="gdhA"
                     /locus_tag="WGR19_001941"
     CDS             1779..3113
                     /gene="gdhA"
                     /locus_tag="WGR19_001941"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0799402.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT          881 a          921 c          797 g          722 t
ORIGIN      
        1 ttgcgcgatg ccgttcaagg acaaacccat acccttttcg gcaaaacgga tttcacggtc
       61 gtcaaacgag actttgccga agccgacccg tttcagggct tcgtccacgc tgttttgagg
      121 aggcggcgtt tccgcatcgg gacgggcggc aaaataatcg gcatacagtt tccacaacgc
      181 ctgcactgtc ggatcgaacg cgccgtccgt cagcgcgtga atatcgcggc acaggctcaa
      241 cagttccaaa aaatccgccg acggcgaagt cagataaccg tccctgttca ggcggctgat
      301 caggctgtct tcacggtaaa ggctgaacaa ttttttccaa acgcgccact tccgccaaaa
      361 ccttgttgac caaatccgcc gcacgcctgt cgtccacacc gaacagacgg agctccgcac
      421 cggaacccag tgcgacacct ttccagaaaa acacattttc attgcgtttt tcatccccgt
      481 tgcgtttttc atcatcggcg gcaaaaggat tcggcaggaa agaaaccgcc gcgcccgccg
      541 ccgcaacggc ggcaaccgtc agaaaacgcc tgcgcccgaa atgcctgcct ataccgcctc
      601 ctaaaccgac actgccgcct tgatatgcgg atgagggtcg taaccttcca actcgaaatc
      661 ttcaaacttg aaggaaaaca aatctttgac ttcaggattg attttcatca ctggcaaggc
      721 gcgcggttcg cgttccaact gcaatgcggc ctgctcgaaa tggttgcggt acaaatgcgc
      781 gtcgccaaac gtatggacaa actcgcccgc ctccaatccg cacacttgcg ccatcatcat
      841 ggtcaacaat gcgtagctgg caatattaaa cggcacacca aggaaaatat ctgcactacg
      901 ctggtaaagc tggcaggaca gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca
      961 gggcggcaag gccatttcat cgaccaaagc cggattccac gccgatacaa tcaggcggcg
     1021 cgagtcggga ttcttcttga tttgttccag cacattggcg atttggtcga tatgcctgcc
     1081 gtcgggcgcg ggccagttac gccactggta gccgtaaacc gggcctaagt cgccgttttc
     1141 gtccgcccac tcgtcccaaa tggaaacatt gttgtccttt aggtatttga tattggtatc
     1201 gcctttgaga aaccaaagca gctcgtggat aatcgaacgc agatgcagct ttttggtcgt
     1261 cagcagcgga aaacctttgc ccaagtcaaa acgcatctga taaccgaata cggagcgcgt
     1321 acccgtaccg gtgcggtctg atttgtccgt accgttgtcg aggacgtggc gcatcaagtc
     1381 caaataggct ttcatagcag tctttcatca aattaaacgg cgcatattgt aacatttccg
     1441 gataatgccc aaaacacgga tacaggcagg caggattgtt ggcaatttca gtccttttcc
     1501 acagtaaaac ccggtgggaa aacaaaatta ccttgattgg aatcaaaaaa tctagtttaa
     1561 ttacttagaa taaaatttca ataatatttt tatttacgaa attaatttat gattattgat
     1621 tgattatcag caacaaacat caaacatcga aaatatggaa aaaataatgt caacaatttt
     1681 tgccaaatcg ggcttggcat cagaaaaaaa taggtttata ttcccaccta caaatttgtt
     1741 ttcccattag tacactatca accaaaagga gtatccgaat gactgacctg aacaccctgt
     1801 ttgccaacct caaacaacgc aatcccaatc aggagccgtt ccatcaggcg gttgaagaag
     1861 tcttcatgag tctcgatccg tttttggcaa aaaatccgaa atacacccag caaagcctgc
     1921 tggaacgcat cgtcgaaccc gaacgcgtcg tgatgttccg cgtaacctgg caggacgata
     1981 aagggcaagt ccaagtcaac cggggctacc gcgtgcaaat gagttccgcc atcggtcctt
     2041 acaaaggcgg cctgcgcttc catccgaccg tcgatttggg cgtattgaaa ttcctcgctt
     2101 ttgaacaagt gttcaaaaac gccttgacca ccctgcctat gggcggcggc aaaggcggtt
     2161 ccgacttcga ccccaaaggc aaatccgatg ccgaagtaat gcgcttctgc caagccttta
     2221 tgaccgaact ctaccgccac atcggcgcgg acaccgatgt tccggccggc gacatcggcg
     2281 taggcgggcg cgaaatcggc tacctgttcg gacaatacaa aaaaatccgc aacgagtttt
     2341 cttccgtcct gaccggcaaa ggtttggaat ggggcggcag cctcatccgt cccgaagcga
     2401 ccggctacgg ctgcgtctat ttcgcccaag cgatgctgca aacccgcaac gatagttttg
     2461 aaggcaaacg cgtcctgatt tccggttccg gcaacgtggc gcaatacgcc gccgaaaaag
     2521 ccatccaact gggtgcgaaa gtgctgaccg tttccgactc caacggcttc gtcctcttcc
     2581 ccgacagcgg tatgaccgaa gcgcaactcg ccgccttgat cgaattgaaa gaagtccgcc
     2641 gcgaacgcgt tgccacctac gccaaagagc aaggtctgca atactttgaa aaccaaaaac
     2701 cgtggggcgt cgccgccgaa atcgccctgc cctgcgcgac ccagaacgaa ttggacgaag
     2761 aagccgccaa aaccctgttg gcaaacggct gctacgtcgt tgccgaaggt gcgaatatgc
     2821 cgtcgacttt gggcgcggtc gagcaattta tcaaagccgg catcctctac gccccgggaa
     2881 aagcctccaa tgccggcggc gtagcaactt caggtttgga aatgagccaa aacgccatcc
     2941 gcctgtcttg gactcgtgaa gaagtcgacc aacgcctgtt cggcatcatg caaagcatcc
     3001 acgaatcctg tctgaaatac ggcaaagtcg gcgacacagt aaactacgtc aatggtgcga
     3061 acattgccgg tttcgtcaaa gttgccgatg cgatgctggc gcaaggcttc taagcaaacg
     3121 ccgccgctcc gcaaacaaaa tgccgtccga accgcaaatg ctgttcagac ggcatttcat
     3181 tatccgcccg ttcgaatcgg gtgatactat ttgtatccag aattgttgga tagtttggga
     3241 gtggagagga agaaatgatt atggaccaat actatatgga gttaaagaat aagttatcta
     3301 acagaccaat cctactggat a
//
LOCUS       DAWFJW010000151         3302 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFJW010000151 DAWFJW010000000
VERSION     DAWFJW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.151"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(73..810)
                     /gene="ubiE"
                     /locus_tag="WGR19_001942"
     CDS             complement(73..810)
                     /gene="ubiE"
                     /locus_tag="WGR19_001942"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0799403.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(853..1221)
                     /locus_tag="WGR19_001943"
     CDS             complement(853..1221)
                     /locus_tag="WGR19_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0799404.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            1341..2960
                     /gene="mnmC"
                     /locus_tag="WGR19_001944"
     CDS             1341..2960
                     /gene="mnmC"
                     /locus_tag="WGR19_001944"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0799405.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQAMQCLQFDSINLIEHILPDVRFWLVPPSRTHHLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(2957..3106)
                     /locus_tag="WGR19_001945"
     CDS             complement(2957..3106)
                     /locus_tag="WGR19_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799406.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT          855 a         1095 c          655 g          697 t
ORIGIN      
        1 tctctttctc aaaaaacaca aaaatgccgt ctgaaacgtg ttcagacggc attggctgca
       61 cagccagtcc gtttaaaatt tcacgccctt atgcagcgcg acgatgcccg cactcatatt
      121 gtgataatcc acgctgtcaa agcccgcatc cagcatcatc tgtttcaaag tttcctgatc
      181 ggggtgcata cggatggatt cggcgagata ttgataactc tccgcatctt tggcaatcag
      241 tttgcccatg accggcagca gcttgaacga atagaaatca tacgcgcctt ccaaaggttt
      301 gtagattttg gaaaactcca ataccagcaa tgtcccgccg ggtttcaaaa cacggtacat
      361 ctctttcagc gcggcatctt tatgcgtcat attccgcaaa ccgaacgcca cggaaaccaa
      421 gttgaaataa ttgtcaggga aaggcagttt ttccgcatcg gcaagcgaca cgggcaaaat
      481 catgccttcg ttcaaaagac ggtcgcgccc gacggtcagc atagaggaat taatatcggt
      541 cagccaaacc tcgccttcct tgccgacccg tttcgcccaa ccgcgcgaca aatcgcccgt
      601 accgccggca atgtccaaca ctttatcgcc ttttttcagg tgcgccgtgt tgatggtgaa
      661 atgcttccac accctgtgca gccctgccga catcacatcg ttcataatgt cgtagttttt
      721 ggcgacggag tggaacactt ccgccacttt tccggctttc tcatcttcgt tgaccgtact
      781 gaatccgaaa tgcgttttct gtccgcccat attctaacca cctccctgat gccgccctac
      841 ggcatcccaa acttaacaat ctgattgctt ttcaaaacgg gaagcacccg ccgccgccaa
      901 tttgtccaaa tattcctgcc acatcgcatc gtatccgtat gccagtctgt gcagatacgc
      961 ccaatcgtaa agaccgctgt cgtgcccgtc tgaaaaactg attttcagcg cgtactgtcc
     1021 gacaggctgc aaatccgcga tttggacatc cgccttgccg gtctgcaaaa catcctgtcc
     1081 cacgccgtgt ccgcgcactt ccgcactcgg cgaatagacg cgtaaaaatt cggcaggcag
     1141 atttttaggt tcgtccccat aagtcaaaac caacacggta cggtttttca acaaacggat
     1201 ttcaacagga atattattca aaaaacacct cggttcggca tacaatatcc ggcaggcgca
     1261 ggcgtgcctt caaacggcgc gtattttaac agctttatcc cgataccgat attcaaaccc
     1321 acactttaaa cggcattgat atggacaatc tcgtatggga cggcattccc gacatccgca
     1381 cactcgacca agccatccgc aaacacgcac ccccgctcaa cctgattatc tgcctccccg
     1441 ataatcagat tcccgatttt caaaccgcac aagatgcttc ggacgcggaa tgccgtctga
     1501 agcaccgttt ggatcaggca atgcagtgcc tccagttcga cagcatcaac ctcatcgaac
     1561 acatcctgcc cgatgtccgc ttctggctgg ttcccccttc acgcacccac cacctgcacg
     1621 aacatttcca ccacatttcc tggcagaccg aagccatccc gcaaaccgaa agcaagcccg
     1681 acaaaccctg gtttgcactt ccacaaacat ccgaacggca aaaaccggaa cacatcctcg
     1741 ttatcggcgc gggcatatcc ggcgcggcaa ccgcccacgc cttagcatca cacggcattt
     1801 ccgttaccgt attggaagcc cgaaaagccg cccaagccgc cagcggcaac cgccaagggc
     1861 tgctctacgc caaaatctcg ccgcacgaca ccgaacagac cgaacttttg cttgccggct
     1921 acggctacac caaacgcctg ctcggacaca tcctgcccga atccgaaacc tggggcggca
     1981 acggcatcat ccacctcaat tacagccgca ccgaacaaca acgcaatcac gaattgggtt
     2041 tgcaaaaaca ccataaccac ctctaccgca gcatcacatc tgcagaagcc gaaaaaatcg
     2101 ccggtatccc actgtccgtc ccatacgacc acccttcatg cggactctac tggcaacacg
     2161 gcgtatggct caatccaccc gcattcgtcc gcaccctcct caaccatccg ctcattggac
     2221 tacacgaaga cacacccttg accgacattt cccacgacgg ggaaaagtgg attgcaagca
     2281 cgccaaacgg cacatttacc gccacacaca tcatctactg caccggtgcg aacagcccct
     2341 acctacccga aaccaacctc gccgccctgc ctctcaggca aatacgcgga caaaccggcc
     2401 tcacaccgtc caccccgttt tccgaacaac tgcgttgcgc cgtttcaggc gaaagctaca
     2461 tcagcccgtc gtggcacgga ctgcactgct acggcgcgag ttttattccc aacagcagcc
     2521 ataccggatg gaacgaagcc gaagaagcct caaaccgcca agcattggca caccttaacc
     2581 ccgccctttc cgaatcattg tttgccgcca acccaaaccc ccaaaaacac caagggcacg
     2641 ccgccatacg ctgcgacagc cccgaccacc ttcccctagt cggcgcactc ggcgacattg
     2701 ccgctatgca acaaacttac gccaaactcg cgctggacaa aaactatcgc atcgatgccc
     2761 catgcccata tctgcctaat gcctacgcca acaccgccca cggcacacgc gggcttgcca
     2821 ccgcccccat ctgcgccgcc gccattgcag cccaaatcct aggcttgccc catccctttt
     2881 cacaacgcct gcgccacgcc ctacacccca accgcaccat catccgcgcc atcgtcagaa
     2941 ggaaggatct aaccccttaa gcctttgccg catgccgtcc cgaccacaga aaatcatatt
     3001 caaataaaca taccaatcct gacggtaaaa tatcgaaaac acccataacg ttttcttccc
     3061 aaaataagga gtaaggaatg cctaacaaat tgcctttgct gtccaagatg aatgttccgt
     3121 aacattcgaa gacttcctta accctgccag ccgtttcggt ttctgtaccc cctgttagat
     3181 aaggaaggaa tgaaaaagcc ctgttgattg tatgaagtaa agggcgttaa tttttgttaa
     3241 tcttcccttc tcaggaactc aatatataag gatgttaatt tttatttccc ttctcgagga
     3301 ct
//
LOCUS       DAWFJW010000152         3283 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFJW010000152 DAWFJW010000000
VERSION     DAWFJW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.152"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            163..1584
                     /locus_tag="WGR19_001946"
     CDS             163..1584
                     /locus_tag="WGR19_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0799407.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANTPPARLSATAESASALLAAAVCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHWKDLHKLFHYLKKQGFPL"
     gene            1581..2651
                     /locus_tag="WGR19_001947"
     CDS             1581..2651
                     /locus_tag="WGR19_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0799408.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NALPPPEFHFRKTKKECAVLGTLSRLNTVRAVHLMLDIFKKMVDRDMPVRLNVAGIGE
                     EMDNLKAQAKRLGIEDKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEA
                     GLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPELREAMGKRLH
                     AHVAALCSDDEIYRTTMAAYTM"
     gene            2671..>3283
                     /gene="pglH"
                     /locus_tag="WGR19_001948"
     CDS             2671..>3283
                     /gene="pglH"
                     /locus_tag="WGR19_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ0799409.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLHVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALIAVSQTYLDRAKEANPNVPGETVYIGTDFAA"
BASE COUNT          654 a         1098 c          846 g          685 t
ORIGIN      
        1 cgataaaaaa cggccccgtt cagaccgccg cccgcccgga aaagcgcgac ccggactgcc
       61 gcaccccgcc ggcagccgcg acggtctgaa agccgtcaaa ttccgatcaa gaaaggcttc
      121 agacgacaca ggcagcatcc cgccgccgcc cggacatcaa aaatggacac aaaagaaatc
      181 ctcggctacg cggcaggctc gatcggcagc gcggttttag ccgtcatcat cctgccgctg
      241 ctgtcgtggt atttccccgc cgacgacatc gggcgcatcg tgctgatgca gacggcggcg
      301 ggactgacgg tgtcggtatt gtgcctcggg ctggatcagg catacgtccg cgaatactat
      361 gccgccgccg acaaagacac cttgttcaaa accctgttcc tgccgccgct gctgtctgcc
      421 gccgcgatag ccgccctgct gctttcccgc ccgtccctgc cgtctgaaat cctgttttca
      481 ctcgacgatg ccgccgccgg catcgggctg gtgctgtttg aactgagctt cctgcccatc
      541 cgctttctct tactggtttt gcgtatggaa ggacgcgccc ttgccttttc gtccgcgcaa
      601 ctcgtgccca agctcgccat cctgctgctg ctgccgctga cggtcgggct gctgcacttt
      661 ccagcgaaca ccgccgtcct gaccgccgtt tacgcgctgg caaaccttgc cgccgccgcc
      721 tttttgctgt ttcaaaaccg atgccgtctg aaggccgtcc ggcgcgcacc gttttcatcc
      781 gccgtcctgc atcgcggcct gcgctacggc ataccgatcg cactaagcag catcgcctat
      841 tgggggctgg catccgccga ccgtttgttc ctgaaaaaat atgccggcct agaacagctc
      901 ggcgtttatt cgatgggtat ttcgttcggc ggggcggcat tattgttcca aagcatcttt
      961 tcaacggtct ggacaccgta tattttccgc gcaatcgaag caaacacccc gcccgcccgc
     1021 ctctcggcaa cggcagaatc cgcctccgcc ctgcttgccg ccgccgtctg cctgaccggc
     1081 attttctcgc ccctcgcctc cctcctgctg ccggaaaact acgccgccgt ccggtttatc
     1141 gtcgtatcgt gtatgctgcc gccgctgttt tgcacgctgg tagaaatcag cggcatcggt
     1201 ttgaacgtcg tccgaaaaac acgcccgatc gcgctcgcca ccttgggcgc gctggcggca
     1261 aacctgctgc tgctggggct tgccgtaccg tccggcggcg cgcgcggcgc ggcggttgcc
     1321 tgtgccgcct cattctggtt gttttttgtt ttcaagacag aaagctcctg tcgcctgtgg
     1381 cagccgctca aacgcctgcc gctttatacg cacacattgc tctgcctgac ctcctcggcg
     1441 gcctacacct gcttcggcac accggcaaac taccccctgt ttgccggcgt atgggcggta
     1501 tatctggcag gctgcatcct gcgccactgg aaagatttgc acaaactgtt tcattatttg
     1561 aaaaaacaag ggtttccatt atgaaaatca tcctgaccac ttctatgtcg ggcttgggcg
     1621 gcacggaaac cgccaccgtc cgcctcgggc ggctgctcaa acggcgcggg cacgacatca
     1681 tccttgcttc ttcggacggg ccttttgtcg gagaggcgca agcatcgggc atccgttggc
     1741 agccggttga tttttaccgg ggcgggcttg ccggctacct caaaagcacc tttgcctacg
     1801 cccggatgct gcgccgcgaa caacccgaca tcatcgactg ccaaatggcg cgcgttgttc
     1861 cagcctgcgc ccttgccgcc aaaatcgttt cgccgaaaac caaaatcatc taccactcgc
     1921 acggtttgga cgcggcaacc taccccaaaa tcgccaagct cttcgacaag ctgggcgtgt
     1981 acatcatcgg caactgtaaa cacgagcgcg aaaaactcat ccgccacggc ttccccgccg
     2041 gccggattgc ctacgcctac aacgcactcc cccccccgga atttcatttc cggaaaacca
     2101 aaaaagaatg cgccgtactc ggcacgcttt cccgtttgaa caccgtccgc gccgtgcatc
     2161 tgatgctgga cattttcaaa aaaatggtcg accgcgatat gcccgtacgc ctgaacgtgg
     2221 cgggcatagg cgaagaaatg gacaacctca aagcccaagc caaacgtttg ggtattgaag
     2281 acaaagtcac cttcctcggc ggcgtgcgcg atttgaccgc ctacttcaaa gacgtggata
     2341 ttttggtgaa cacgccccac tgcatcggcg accacggcgc aggtgtaggc aacaacatct
     2401 tagaagccgg actttacgac acgcccgtcg ttacctacga tatggcgggc atttccgaaa
     2461 tggtcgtcaa cggggaaacg ggttactgca tccccttcgg cgacgaagaa gcctttatcg
     2521 ccgccgtaga cacgctcgtc agacaaccgg agttgcgcga agcaatgggc aaaaggctgc
     2581 acgcacacgt cgccgcactc tgttccgacg acgaaatcta ccgcaccacc atggccgcct
     2641 ataccatgta atgccgtctg aaagccgccg atgaacatca ccatagtcgc cccctactgc
     2701 tcgctgccgt ccgaaccgta tttcaaccgc ttttggtatc tggcagaacg attgtcgcaa
     2761 tcgcacgacg tgttgctgat taccagccgt ttccgccact acgacaaatc cttcagacgg
     2821 catgaagatg ctgccgccac ttcaaacggc aggctgcacg tcaagctgct ggacgaaccc
     2881 ggctaccgaa aaaacgtctc cctcgcccgc gtcgccagcc accgcgtatt cgtccgcaat
     2941 ttggcacgct ggctgcacag cccgcaggcg gcggaacaag atatcgtcta ttccgcctat
     3001 ccgctgatgg caaccaacct gctgttgggc aaacacaaag cgcgtttggg ctacaaattg
     3061 attgtcgatg tgcaggacgt atggccggag tctttctcct cggtcgtacc gtttttgaaa
     3121 aaagtaccgc acaagctgct gccctttgct tcacgcgcca accgtgccta ccgctgcgcc
     3181 gacgcgctga ttgccgtatc gcagacttat ctcgaccgcg ccaaagaagc caatccgaac
     3241 gtacccggcg aaaccgtcta tatcggtacg gattttgccg cca
//
LOCUS       DAWFJW010000153         3228 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFJW010000153 DAWFJW010000000
VERSION     DAWFJW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.153"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..270
                     /locus_tag="WGR19_001949"
     CDS             <1..270
                     /locus_tag="WGR19_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0799410.1"
                     /translation="RIGKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPWTIYVG
                     VDCKPLKRRSQDKILELEKHLVAEFNLDLCPIIPSPTRPNPKETR"
     gene            267..830
                     /gene="gmhB"
                     /locus_tag="WGR19_001950"
     CDS             267..830
                     /gene="gmhB"
                     /locus_tag="WGR19_001950"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0799411.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            859..1602
                     /locus_tag="WGR19_001951"
     CDS             859..1602
                     /locus_tag="WGR19_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0799412.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEVELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1599..2291
                     /locus_tag="WGR19_001952"
     CDS             1599..2291
                     /locus_tag="WGR19_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0799413.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLAPTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTDIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQW
                     LKIHGRELFALG"
BASE COUNT          796 a          998 c          765 g          669 t
ORIGIN      
        1 agaattggaa aacatgcaat tttaggaaca aatgtaacag tattaccagg cgttactatt
       61 ggtgatgggg ttgctgttgg agcaggggct atagtgagaa aaaatcttga gccgtggact
      121 atttatgtcg gggtagattg taaaccactg aagcgcagat cacaagataa aatattggag
      181 ttagagaaac atcttgtagc agaatttaat ctagatttat gtcctataat cccatccccg
      241 acacgcccca acccaaagga aacgcgatga aactcatcat tctcgaccgc gacggcgtca
      301 tcaatcagga ccgcgacgac ttcgtcaaat ccgttgacga gtggatacct gtcgaaggca
      361 gcatggatgc ggtggcattt ctgacgcagg caggctacac cgtcgccgtt gccaccaacc
      421 aatccggcat cgggcgcaaa tattttaccg ttcaaaacct taccgaaatg cacgccaaaa
      481 tgcaccgcct cgtccgtcag gcaggcggcg aaatcaacgg catctggttc tgcccgcaca
      541 ccgatgccga caactgcaac tgccgcaagc ccaaaccggg tatgattgaa gacatcatcg
      601 gacgcttcaa cgcccaagcc tcggaaacct ggctggtcgg cgacagcctg cgcgatttgc
      661 aggcaatcga tgccgtcggc ggaaaacccg cgctggttct gaccggaaaa ggcaaaaaaa
      721 cgctctccca acacggacac gaattacccg aacacacaca ggttttcgat accctgctcg
      781 atttctcaca atacatcatg caggaaaaca ccgcaccgca agccgactga acataccgca
      841 ttccgacaag gcaaaaccat gctcatcatc cgcaacctga tttactggct gatactctgt
      901 tccagcctga ttttcctctt tccctttatg ctgctcgcct cgcctttccg agacggggcg
      961 cacaagatgg cgcgggtctg ggtcaaaatc ctcaacctct cgctcaaaca catcgtcggg
     1021 ctcaaatacc gcatcatcgg cgcggaaaac atccccgacc gccccgccgt catctgcgcc
     1081 aaacaccaaa gcggctggga aacgctcgcc cttcaggaca tttttccgcc gcaggtttac
     1141 gttgccaaac gcgagttgtt caaaatcccc tttttcggct ggggtttaaa actggtcaaa
     1201 accataggca tagaccgcaa caaccgccgc gaagccaacg agcagctcat aaaacagggg
     1261 ttggcgcgca aaaacgaagg ctattggatt accattttcc ccgaaggcac gcgccttgcg
     1321 cccggaaaac gcggcaaata caaactcggc ggcgcgcgca tggcgaaaat gtttgagatg
     1381 gacatcgtcc ccgtcgccct caacagcggc gaattttggc cgaaaaactc ctttctgaaa
     1441 tatccggggg aaatcaccgt cgtcatctgt ccgaccatcc cgcacgcaag cggcagcgaa
     1501 gtcgaattga tggaaaaatg cgaacacctc atcgaaacgc agcagccgct catttccggc
     1561 gcaggtccgt ttgccgccaa aatgccgtct gaaaccgcat gaccgccttt gtccacaccc
     1621 tttcagacgg catggaactg accgtcgaaa tcaagcgccg tgccaagaaa aacctgatta
     1681 tccgccccgc cggcacacat accgtccgca tcagcgtccc accctgcttc tccgtctccg
     1741 ctctaaaccg ctggctgtat gaaaacgaag ccgtcctgcg gcaaacactg gcgaaaacac
     1801 cgccgccgca aactgccgaa aaccggctgc ccgaatccat cctcttccac ggcagacagc
     1861 ttgcccctac cgcccatcaa gacacgcaaa tcctgctgat gccgtctgaa atccgtgttc
     1921 ccgaaggcgc acccgaaaaa cagcttgcgc tgctgcggga ctttttggaa cggcaggcgc
     1981 acagttacct gattccccgc ctcgaacgcc acgcccgcac cacacaactg ttccccgcct
     2041 cctcctcgct gacctctgcc aaaaccttct ggggcgtgtg ccgcaaaacc acagacatac
     2101 gcctcaactg gcggctggtc ggcgcaccgg aatacgttgc cgattatgtc tgcatacacg
     2161 aactctgcca cctcgcccat cccgaccaca gccccgcctt ttgggaactg acccgccgct
     2221 tcgcacccta cacgcccgaa gcgaaacagt ggctcaaaat ccacggcagg gaacttttcg
     2281 ccttgggctg acacggtccc gggccggccc gccgctttca gacggcatcc gtgccggaac
     2341 aggcacgcgc ccgcccgatt caaaccgcga tgacgctttg ccgccggttc ggggcaggat
     2401 ggcggcacac acgccgtctg ccgcgtttca tttcacacca ctcttccgaa acccgaaacc
     2461 cgcccggtcc gacgtgcggt atgaaacgct taagctgacg cgaagtcttt tactgatttg
     2521 cccgcgaaaa tgccgtctga aaggttttcg gacggcattt ttttgcgttt cccaggatgg
     2581 cggcggattc gtaaaaggcg gtcagggtgg attgtaggat gggttgagac ctgccgaatc
     2641 cgccgcatct gccaaatcta ccgccgtcat tcctacgaaa gtgggaatct agaacgcggg
     2701 gttaagaaaa cctgcatccc gtcattccca cgaaagtggg gatccagttt tttgagtttc
     2761 agtcatttcc gataaattgc cttagcattg aatgtctaga ttcccgcctg cgcgggaatg
     2821 acgaatccat ccatacggaa acctgcaccg cgtcatgcct acgaacctgc attccgtcat
     2881 tcccacgaaa gtgggaatcc agttttttga gtttcagtca tttccgataa attgccttag
     2941 cattgaatgt ctagattccc gcctgcgcgg gaatgacgaa tccatccata cggaaaccta
     3001 catcccgtca ttcctacgaa cctacattcc gtcattccca cgaaagtggg aatccagaat
     3061 cccagacttt cagataatct ttgaatattg ctgttgttct aaggtctaga ttcccgcctg
     3121 cgcgggaatg acgggatttg aggtttctgt tcgcgtcatt cccacgaacc tgcatcccgt
     3181 cattcccacg aaagtgggaa tctagttttg tcggtgcgga aacttatc
//
LOCUS       DAWFJW010000154         3212 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFJW010000154 DAWFJW010000000
VERSION     DAWFJW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.154"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>3212
                     /locus_tag="WGR19_001953"
     CDS             <1..>3212
                     /locus_tag="WGR19_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ0799414.1"
                     /translation="QAGKDINIIAGQISNQSEQGQTQLQAGRNINLDTVQTGKHQETH
                     FDADNHVIRGSTNEIGSNIQTQGDVTLLAGNNLNAKAAQVSSANGTLAVSAKNDINIS
                     AGINTTHVDDASKHTGRSGGGNKLIITDKAQSHNETAQSSTFEGKQVVLQAGNDANIL
                     GSNVISDNGTQIQAGNHVRIGTTQTQSQSETYHQTQKSGLMSAGIGFTIGSKTNTQEN
                     QSQSNEHTGSTVGSLKGDTTIVAGKHYEQIGSTVSSPEGNNIIHAQSIDIQAAHNKLN
                     SNTTQTYEQKGLTVAFSSPVTDLAQQAIAVAQSSKQVGQSKNGRVNAMAAANAGWQAY
                     QTGKSAQNLANGTTNAKQVSISITYGEQQNRQTTQVQASQAQASQIQAGGKTTLIATG
                     AAEQSNINIAGSDVAGKAGTILIADNDITLQSAEQSNTERSQNKSAGWNAGAAVSFGQ
                     GGWSLGVTAGGNVGKGYGNGDSVTHRHSHIGDKGSQTLIQSGGDTTIKGAQVRGKGVQ
                     VNAKNLSIQSVQDRETYQSKQQNASAQVTVGYGFSASGDYSQSKIRADHASVTEQSGI
                     YAGEDGYQIKVGNHTDLKGGIITSSQSAEDKGKNRFQTATLTTSDIQNYSQYQGKSFG
                     LGASVAVSGKTLGQGAQNKPQNKHLTSVADKNGASSSVGYGSDSDSQSSITKSGINTR
                     NIHITDEAGQIRLTGKTAAQTKADIDTNVTTDTAERHSGSLKNTFNKEAVQSELDLQR
                     EVTQEFDKTRQGVKQELYAVVDSKREQATKERLKNGGYDNDKSRALNKEANELDEKIR
                     WLDAGLGLVWGAGSSDMAWSMFATTQADRAVRSATAPKEMWFHKKIIDEKTGKVSFDT
                     RQIWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNSTNGSGVIA
                     VMNPPTGKYKSDSNNKIKDSLWLSSSLVSELMYVGYDQLNNKVFQGYLPKTNSEKLNQ
                     DIYREVQKMDNGWSVDTSNHSRGGITASVSLKDWVNNKEQNGIAPIRKARFYGTATNV
                     KNDYADVLQKNGYTYTGADGKTYNSGAYSIVHDKDFVGNK"
BASE COUNT         1163 a          751 c          705 g          593 t
ORIGIN      
        1 gcaggcaggc aaagacatca acatcatcgc cggtcaaatc agcaatcaat cagagcaagg
       61 gcaaacccag ctgcaagcag ggcgcaacat caatctggat accgtacaaa ccggcaaaca
      121 tcaagaaacc cattttgatg ctgataacca tgttattcgc ggttcaacta acgaaattgg
      181 tagcaatatt caaacgcaag gtgatgttac cctgttagca ggcaataacc tcaatgccaa
      241 agctgcccaa gtcagcagcg caaacggtac actcgctgtg tctgccaaaa atgacatcaa
      301 catcagcgca ggcatcaata cgacccatgt tgatgatgcg tccaaacaca caggcagaag
      361 cggtggtggc aataaattga ttattaccga caaagcccag agccataacg aaaccgctca
      421 aagcagcacc tttgaaggca agcaagttgt attgcaggca ggaaacgatg ccaacatcct
      481 tggcagcaat gttatttccg ataatggcac ccagattcaa gcaggcaatc atgttcgcat
      541 tggtacaacc caaactcaaa gccaaagcga aacctatcat caaacccaga aatcaggatt
      601 gatgagtgca ggtatcggct tcactattgg cagcaagaca aacacacaag aaaaccaatc
      661 ccaaagcaac gaacatacag gcagtaccgt aggcagcttg aaaggcgata ccaccattgt
      721 tgcaggcaaa cactacgaac aaatcggcag taccgtttcc agcccagaag gcaacaatat
      781 catccatgcc caaagcatag acattcaagc ggcacacaac aaattaaaca gtaataccac
      841 ccaaacctat gaacaaaaag gcttaacggt ggcattcagt tcgcccgtta ccgatttggc
      901 acaacaagcg attgccgtcg cacaaagcag caaacaagtc ggacaaagca aaaatggtcg
      961 tgtcaatgcc atggcagctg ccaatgcagg ctggcaagcc tatcaaacag gtaagagtgc
     1021 acaaaactta gccaatggta caaccaatgc caaacaagtc agcatctcca taacctacgg
     1081 cgaacagcaa aaccgacaaa ccacccaagt tcaagcaagc caagcccaag cgagtcaaat
     1141 tcaagcaggc ggtaaaacca cattaatcgc cacaggcgca gcagaacaat ccaatatcaa
     1201 catcgcaggc tcagatgttg ccggcaaagc aggcacaatc ctgattgccg ataacgacat
     1261 cacactccaa tcagccgagc aaagcaatac cgaacgcagc caaaacaaat cggcaggctg
     1321 gaacgcaggt gctgccgtat cattcggaca aggaggctgg tcattaggcg ttaccgcagg
     1381 cggcaatgtc ggcaaaggct acggcaatgg cgacagcgta acacaccgcc acagccatat
     1441 cggcgacaaa ggcagccaaa cccttatcca aagtggtggc gatactacca tcaaaggcgc
     1501 gcaagtacgc ggcaaaggcg tacaagtcaa tgccaaaaac ctaagtattc aaagcgtaca
     1561 agatagagaa acttatcaaa gcaaacaaca aaacgccagt gcacaagtga ccgtaggtta
     1621 tggcttcagt gccagtggcg attacagtca aagcaaaatc cgagccgacc atgcttcagt
     1681 aaccgagcaa agcggtattt atgccggaga agacggctat caaatcaagg tcggaaacca
     1741 tacagacctc aaaggcggca tcatcaccag cagccaaagc gcagaagaca agggtaaaaa
     1801 ccgctttcag acggccaccc ttactaccag cgacattcaa aattacagcc aataccaagg
     1861 aaaaagtttt ggattgggtg ccagcgttgc cgtaagcggc aaaacactgg gacagggcgc
     1921 acaaaataaa cctcaaaaca aacacctgac aagcgtagcc gataaaaacg gcgcaagttc
     1981 atcagtgggt tatggcagcg acagcgacag tcaaagcagc atcacgaaaa gcggcatcaa
     2041 cacccgcaac atacacatca ccgacgaagc gggacaaatc aggctgacag gcaaaacagc
     2101 ggcacaaacc aaagccgata ttgatacaaa cgtaaccaca gacaccgccg aacgacattc
     2161 gggcagcttg aagaacacct tcaacaaaga agcggtgcaa agtgaactgg atttacaaag
     2221 ggaagtaacc caagagtttg ataaaactag acaaggagtg aaacaagaac tttatgcagt
     2281 agtagatagc aaacgagagc aagccacaaa agagagactc aaaaatggtg gatatgacaa
     2341 tgataaatct agagcattaa ataaggaagc taatgaatta gatgaaaaaa ttcgttggct
     2401 tgatgcggga ttaggactcg tttggggagc agggagttct gatatggctt ggagtatgtt
     2461 tgccacaacc caagccgata gagcggtaag gtctgcaact gcacctaaag aaatgtggtt
     2521 ccataagaag ataatagatg aaaaaacagg taaagtatcc tttgatacca gacaaatttg
     2581 gtcattgaat gatttaagca aggaagaact ggcaagcatt caagacacaa atggcaaagt
     2641 tattactgtg tctaatcctg gtattttcaa taatcgagaa gattcattaa gcaatgcagc
     2701 aaaacaaaat cgtaatagta caaacggtag tggtgttatt gcagttatga atcctccaac
     2761 agggaaatat aaatctgatt ctaataacaa aataaaagat tctttatggc tcagttcaag
     2821 tcttgtttct gaactgatgt atgtcggtta cgaccaatta aataataaag tgttccaagg
     2881 ctatttaccc aaaaccaatt cagaaaaact gaatcaagat atttatcgag aggttcaaaa
     2941 aatggataat ggctggtctg ttgataccag taaccacagt cgtgggggaa ttacagcaag
     3001 cgtttcccta aaagattggg taaacaataa agagcaaaat ggcattgccc caatcagaaa
     3061 agcacgtttc tatggtacag ccacaaatgt gaagaatgat tacgccgatg ttttacagaa
     3121 aaacggctat acctatacgg gtgcagacgg taaaacttat aacagcggag cctactcaat
     3181 cgtgcatgat aaagattttg tggggaacaa at
//
LOCUS       DAWFJW010000155         3211 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFJW010000155 DAWFJW010000000
VERSION     DAWFJW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.155"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            58..498
                     /locus_tag="WGR19_001954"
     CDS             58..498
                     /locus_tag="WGR19_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0799415.1"
                     /translation="MGNPVDLSEFVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWI
                     DNYVTKQPEKLSVITVKGDSMEGVLNHGDSILINHAKTDPRDGLYVLRIGNDLFVKRV
                     QRIPGKLLVTSENPRYAPFEIDFSNAQDDIAIIGRVEWYGRSID"
     gene            650..1297
                     /locus_tag="WGR19_001955"
     CDS             650..1297
                     /locus_tag="WGR19_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0799416.1"
                     /translation="MSAFFTLFVPCAKTNSPPQENVALFRHNGEILLSGINIKLKSGD
                     SLLIRGPSGCGKTSLLRALAGLWPFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVC
                     YPDIDKQHPELIEAMNTCRLGYLIDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIV
                     LLDEATAALDEPAEAALYRALKQKLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            1402..2070
                     /gene="hda"
                     /locus_tag="WGR19_001956"
     CDS             1402..2070
                     /gene="hda"
                     /locus_tag="WGR19_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0799417.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            2067..2735
                     /locus_tag="WGR19_001957"
     CDS             2067..2735
                     /locus_tag="WGR19_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0799418.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            3108..3211
                     /locus_tag="WGR19_001958"
                     /pseudo
     CDS             3108..3211
                     /locus_tag="WGR19_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          877 a          928 c          724 g          682 t
ORIGIN      
        1 ctggaaaacg ctggagcatt ctctgtaagc agaaattctg atggaattac tgacactatg
       61 ggcaaccctg tcgacttgtc agagtttgta tttgtgccgc gctacaacgt agccgcagca
      121 gcagggtatg gcgcaccggt cttcggcgaa gagcctttgt tctgcttggc tttccggaaa
      181 tattggattg acaactatgt gaccaaacag ccggaaaaac tgtctgtaat taccgttaaa
      241 ggcgattcaa tggagggcgt gctcaatcat ggggattcca ttctcatcaa tcacgccaaa
      301 accgacccgc gtgacggcct atatgtcttg cgtataggca atgacctctt tgtcaaacgt
      361 gtgcaacgca taccgggcaa actgcttgta acgtctgaaa atccacgtta cgcaccgttt
      421 gaaatagatt ttagcaatgc ccaagacgac atcgccatca taggccgtgt tgagtggtat
      481 ggccggagta ttgattaaat ccattttaaa aacctcttaa aacagtttta aaaattccct
      541 aatctcgtca gctttaaaca gaaaaccgcg cattccggcg cggttttgtg aaaaagctgg
      601 cgtaactttt ccggatacaa aaaaacgccg aaatccacgt ctttccgaga tttcagcgtt
      661 ttttactcta tttgttcctt gtgcaaaaac taacagtccc ccacaggaaa acgtcgctct
      721 gttccgccac aacggcgaaa tcctgttgag cggcatcaac atcaaactca aaagcggcga
      781 ttccctgctg atacgcggcc cgagcggttg cggcaaaact tcgttgctgc gcgcgctggc
      841 tgggctttgg ccgttcggca gcagcggcaa agtcagccgt ccgccgcatc aagacatcct
      901 cttcctgccg caacgcccgt acacggcaca gggcagcctg cgcgacgcgg tttgttatcc
      961 cgacatcgac aaacagcatc cggagctaat cgaagccatg aacacctgcc gcttgggtta
     1021 tctgattgac aaattggaca aaacagacga ctggcagcac aaactctccc tgggcgaact
     1081 gcaacgcgtc gccttcgtcc gcgccctgct ttccaagccc aaaatcgtcc tgctcgacga
     1141 agccaccgcc gccttggacg aaccggccga agccgcgctc taccgcgcct taaagcaaaa
     1201 actgcccgac agcatcatca tcagcatcgg acaccgaagc acgctcaacg cgttccacaa
     1261 tatgcgcctc gatgtcggca acgttgcctg cggttgaacg atgccgtctg aagccgtttt
     1321 cagacggcat cgttcaaatt ggaaaggcgc gcgaaaacaa cgtacaataa acccgtttac
     1381 cacccctcgg aatgccgaac cgtgaaccag cttattttcg actttgccgc acacgactat
     1441 ccgagtttcg acaaattcct cggcacggaa aacgcggaac tggtctatgt cctccgacac
     1501 aaacacggac agtttatcta tgtctggggt gaagaaggcg cgggcaaaag ccatcttttg
     1561 caggcgtggg tcgcacaggc gctcgaagcc ggcaaaaacg ccgcctatat cgatgccgcc
     1621 tccatgccgc tgaccgatgc cgcgttcgag gcggaatacc tcgccgtcga tcaagtcgaa
     1681 aaactgggca acgaagaaca agccctgctt ttttccatct tcaaccgctt ccgcaacagc
     1741 ggcaaaggct ttttgctgct cggttcggaa tacacgcccc agcagcttgt catccgcgaa
     1801 gatttgcgga cgcgtatggc gtactgcctc gtttacgaag tcaaaccctt aaccgaccaa
     1861 gaaaaaatcg acgcgctcgc cagtatggcg gcggcacgcc aagtaaccgt cgattccgaa
     1921 attttcgaat acctgctcaa acactggcgg cgcgatatgg acagcctgat gatgatgctc
     1981 gacacgctgg acaactacgc cgtcacaatg ggcaaacgca tcaccctgcc gcttttgcgc
     2041 cagcttttga aacaacagga aacccaatga aaaacctcgc catcttcgac ctcgacaaca
     2101 ccctcatcaa caccgattcc gaccacgcct ggccgcaata cctcatcaaa aaaggcttgg
     2161 ttgacgctgc cgaaaccgaa gcgcaaaacg aaaaattcta ccgcgactac caaaacggct
     2221 gcctcgacat cgacgcattc ctcaaattcc acctcgcccc gctcgcccgt tacagcaaag
     2281 aagagctggc ggaatttcac cgcgaattta tggcggaata catcatcccc cacatctcgc
     2341 ctatgcagcg tatgctggtg cagagccacc aaatggcagg cgacgaaacc ctcgtcatct
     2401 catcgaccaa cgagttcatc atcacccccg tctgccgcct tttcggcatt accaacatca
     2461 tcggcacaca actcgaatcc ggttccgacg gccgctacac cggcaattac atcggcacgc
     2521 ccagcctcaa agaaggtaaa atcacccgcc tgaaccaatg gctcgccgaa cgcggcgaaa
     2581 cgctcgaaag ctacggcaaa acctattttt acagcgactc caaaaacgac ctgccgctgc
     2641 tgcgcctcgt cgacgaaccc gtcgccgtca accccgatgc cgagctggaa aaagaagcca
     2701 aagaaaaagg ctggccggtt ttgaatttca aatgacgcaa tgccgtctga agcccgattc
     2761 ggcgttcaga cggcattgag gctgaaatcc aaaaaactca aagccacagg aatttatccg
     2821 gaaaaacaaa aaacacagga attcatcggg aaaaacaaca atctttccac cgtcattccc
     2881 acgaaagtgg gaatcttgat ccgtccgcac ggaaacttat atttcgtcat ttgcacccga
     2941 cataattcaa tactttattt tgcaataaat taacggatgc tgacaaatta gattggatta
     3001 attaccctat tatccatatt gggttatcgg gttgctaatt gagcagagcg tagcaaacca
     3061 taaagtgagt gtttccaccc actctgcatt tatagtggat taaaatttat agtggattaa
     3121 atttaaatca ggacaaggcg acaaagccgc agacagtaca gatagtacgg caaggcgagg
     3181 caacgctgta ctggtttaaa tttaatccac t
//
LOCUS       DAWFJW010000156         3188 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFJW010000156 DAWFJW010000000
VERSION     DAWFJW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.156"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            358..981
                     /locus_tag="WGR19_001959"
     CDS             358..981
                     /locus_tag="WGR19_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0799419.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1092..2474
                     /locus_tag="WGR19_001960"
     CDS             1092..2474
                     /locus_tag="WGR19_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0799420.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQDGLQCALLQLEGVEDIGFSFDRQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2478..3002
                     /locus_tag="WGR19_001961"
     CDS             2478..3002
                     /locus_tag="WGR19_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0799421.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          651 a          857 c          954 g          726 t
ORIGIN      
        1 ctcaagatat agtggattaa atttaaatca gtacggcaag gcgaggcaac gctgtactgg
       61 tttaaattta atctactata tttccgcctg ctgccgcccg aaaagcgtga tgcgctgatg
      121 gtgctgctgc acaatccgga cgacggatag ccggtgaaaa cgggaaatgc ccgggtggtg
      181 ttgtctgtgc ctgtttggac aagcgttctt gagagcggta aaatcaaggt ttgcccgaaa
      241 agggcagggc gatatgccgc gcttggattt tgtgcgcggt ttttcgcgtt ggaagcaatc
      301 attgtctgtt tgaatattta atgccgtctg aaacgtccgg acgcgccccc gttccctatg
      361 aaaaaaccga ccgataccct acccgttaat ctgcaacgcc gccgcctgtt gtgtgccgcc
      421 ggtgcgttgt tgctcagtcc tctggcgcac gccggcgcgc aacgtgagga aacgcttgcc
      481 gacgatgtgg cttccgtgat gaggagttct gtcggcagcg tcaatccgcc gaggctggtg
      541 tttgacaatc cgaaagaggg cgaacgttgg ttgtctgcca tgtcggcacg tttggcaagg
      601 ttcgtccccg aggaggagga gcggcgcagg ctgctggtca atatccagta cgaaagcagc
      661 cgggccggtt tggatacgca gattgtgttg gggctgattg aggtggaaag cgcgttccgc
      721 cagtatgcaa tcagcggtgt cggcgcgcgc ggcctgatgc aggttatgcc gttttggaaa
      781 aactacatcg gcaaaccggc gcacaacctg ttcgacatcc gcaccaacct gcgttacggc
      841 tgtaccatcc tgcgccatta ccggaatctt gaaaaaggca acatcgtccg cgcgcttgcc
      901 cgctttaacg gcagcttggg cagcaataaa tatccgaacg ccgttttggg cgcgtggcgc
      961 aaccgctggc agtggcgttg attttgaacc cgcgccgcaa ccgaaatacg gcgaatcctg
     1021 tataatccga aaatctgttc actggaagtt cagacggcat tgcaactgtt gatgccgtct
     1081 gaaaaaatat gatggcaaga gacaaccgca tccaaatgtt tccgcacgaa tggcgcgcca
     1141 gtacgacgct ttctggcgtg tacgcgctgc gtatgctggg tatgttcctc gtgctgcccg
     1201 tattggcggt gtatgccgcc tcgctgcccg gcgcggaagg caacaaaacg ctggtcgggc
     1261 tggcaatggg catttacggg ctgacacagg ctctgctgca actgcctttg ggcatcgctt
     1321 ccgacaagtt cgggcgcaag aaaaccattt atgtcgggct gattgtgttt gcagcgggca
     1381 gcttccttgc cgccgccgcc gatacgctgc ccatgctggt cgccgcacgc gccatacagg
     1441 gtgcgggagc ggtcagtgcg gcggttaccg cgctgctggc ggatttgacg cgcgacggcg
     1501 tacgtacccg cgcgatggcg atgatcggtt tgagtatcgg tctgacgttt tcggtcagcc
     1561 tcgtcgttgc ccctgtgatt gccgacgcgg tgggcgtacg cggcttgttt ctgctgaccg
     1621 gtattttgac cgttgccagt atcggcgtgg tggcgtggat gactcccgac ccggaggttt
     1681 ccaagctgca cgaagatacg caggcgcagc cttcgcgcat aggcgaagtt ttgaaaaacc
     1741 gtaggctgct gacgctcgat ttcggcattt tcgccctgca cgccgcgcaa atggcattgt
     1801 ttaccgcgct gccttttgcg atgacgcagc tcggtttgga aaaaatccag cattggaaag
     1861 tctatctgcc ctcgaccatt acgggcttgg tggtgatggt tccgctgatt atcgtcggcg
     1921 agacgcgcaa caagcttaag caggtttttg ttttgggtat cgtctgtatt gcggcggcgc
     1981 agctcggttt gctgtccggt atgcgctcgg taggcttgat taccgcttat ttggttgttt
     2041 actttatcgg ttttaatgtg ttggaagcga gcctgccgtc tatggtttcc aaaatcgcac
     2101 cgtccgacct gaagggtacg gcgatgggcg tgtacaacac gatgcagtcg ctcggactgt
     2161 ttgccggcgg cgcggcaggc ggtttgctgt ttcaaaaata cggcttttcc ggcgtgtttg
     2221 ccttttgcag catattgatg ctgctgtggc tggtgattgc cgttttatcg cctgcgccca
     2281 agcccgtcaa aaacctcagt taccctgtcg gcggcgtgtg gcagggcaat caggacggct
     2341 tgcaatgcgc cctgttgcag ttggaaggcg tggaagacat cggtttcagt ttcgacaggc
     2401 agaccgtcta tctcaaagtg ttgcagaagg gtttcgatca ggctgccgct gaaaaaatca
     2461 tcacaggagt ttaaaaaatg tcattgaaca aagtcatcct catcggccgc ctcggacgcg
     2521 atcccgaagt gcgctatatg cccaacggcg aagccgtctg caatttcagc gtcgccacca
     2581 gcgaaacttg gaacgaccgc aacggtcagc gcgtagagcg caccgagtgg cacaacatca
     2641 cgatgtaccg caaactggcg gaaattgccg ggcaatacct caaaaaaggc gggctggttt
     2701 atctggaagg cagaatccaa agccgcaaat accaaggcaa agacggcatc gaacgcactg
     2761 cttacgatat tgtcgccaac gaaatgaaaa tgttgggcgg gcgcaatgaa aacagcggcg
     2821 gtgcgcctta cgaggaaggt tacggtcaga gtcaggaggc ttaccaacgc cccgcgcagc
     2881 aaagccggca gcccgcctcc gacgcgccgt cccatcccca agaagcacca gccgcgccgc
     2941 gccgccaacc cgtgcctgcc gccgccccgg tcgaggacat tgacgacgac atcccgttct
     3001 gaattttacg gcagcacatc ctcgcggagg gaaatcataa aggacggaga aaccttaaac
     3061 cttacggggc agggtttctc ttttttgcat ttgtgccggc ttgttgctgc cccctgcgtt
     3121 gggcggaacg ttgcgattgc gccgttctgg cgttttgagg cagtgatgcc tgccgcaggc
     3181 ccacgctg
//
LOCUS       DAWFJW010000157         3179 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFJW010000157 DAWFJW010000000
VERSION     DAWFJW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.157"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            56..1009
                     /locus_tag="WGR19_001962"
     CDS             56..1009
                     /locus_tag="WGR19_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0799422.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSICGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1120..1443)
                     /locus_tag="WGR19_001963"
     CDS             complement(1120..1443)
                     /locus_tag="WGR19_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799423.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1616..2977)
                     /locus_tag="WGR19_001964"
     CDS             complement(1616..2977)
                     /locus_tag="WGR19_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0799424.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDAMPEQVHKLFRRSRIVGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MMVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
BASE COUNT          748 a          936 c          765 g          730 t
ORIGIN      
        1 aatctaatcc actatagatt aaaatcacac aatatccaac tattcggacc ttcatatgac
       61 acataccgtc cacctgcatt tagaagaaac cgacaacttg gcgttgcagc gtctgtgcgg
      121 ttcttttgac aacaaccttg atttacttgc taaagcactc gatatccaca tcagccgccg
      181 ttttgaacat ttcactttca acggcgcatt tgcacacgcc ggcaaacgcg cactgctcaa
      241 actcttggaa acggcgcaga cgcgcgacct aaacgacggc gacatcaggc ttgccgccgt
      301 cgaagcccaa accgaagatg ccggtcatca agaaaaaaac catgaccacg cctattattt
      361 ccgcaccaag cgcggcagca tctgcggcag aacgccacgg cagaacggct atatccgcgc
      421 cctactcaac cacgacatcg tattcggtct cgggccggca ggtacgggca aaacctatct
      481 cgcagtcgcc gccgccgtcg atgcgatgga aaaacaccaa gtcgaacgca tcattttagt
      541 gcggcctgcc gtcgaagccg gcgagaaact gggcttcctg cccggagacc tgacccagaa
      601 agtcgatccc taccttcgtc cgctttatga tgccctctat gacctgatgg gctttgaccg
      661 tgtaaccaag ctgattgaaa aaggcctgat tgagattgcc ccgctcgcct atatgcgcgg
      721 caggacgctc aacggcgcat acatcatcct cgacgaagcg caaaacacca cgcccgaaca
      781 aatgaaaatg ttcctgaccc gcatcggctt cggcgcgaaa gccgtcatta ccggcgacac
      841 cagccaaatc gacctgccca aaaacatcaa atcgggatta aaagatgcgc gtgagaaact
      901 gcacaacgtg gaagggctgt atttccacac ctttaccggc gaagacgttg tccggcatcc
      961 tttggtgcaa aaaatcgtcg aagcctacga atcggcagaa cacgactgac attccaatgc
     1021 cgtctgaaac ccgatgcatg gaaaatcagg cagagaaaaa cagatacgga agccaagctc
     1081 cgtatccgta tccgtttctt tatccgtcca accgatacat caatcgaatt ttctgcatcg
     1141 gattgcctca tcttcgcaaa acattgccga agtttcgcgg ttgcacgcct gacgcacttt
     1201 ctgccgcgcc acgccttcat tccgatcgct tgcctcaaac gtcttctgaa aaggcgtaac
     1261 gctgcataca tacatggtgg aatttgaacc gaaaaggtcg attttgacgg tattgccgcc
     1321 gctttccaat acggcgacac ggtgttccaa ctgcgccaga cgtgcttcca aatcgccgat
     1381 tctgtcggca aatgcgggca tggcggcaca cgcggtcaaa atgcccaaaa tacatttttt
     1441 catcaatttt tccttgttgc ttgaaccctg cccacccgga catccttttt ccgagaggca
     1501 gggtcagact ttatttacgg agatgaggcc ccttacaaat caggataata catagaacgg
     1561 cactttatgc ttcaccccat gtgttaaaac ttcattgctc cttgtctgtt gacccttatt
     1621 cccaatccgt tcccacaacc ttcggcttgg gtttgcgcct gcgacggcgg cgttttttcg
     1681 cctgtccttc gtttttttcg tgtcgggcgg cggcttcgtt tttggtcatc tccgaccgct
     1741 gttccgtcga tgccgtctga aacgccgtcc accactcggc aagggcgcgg tccgcattgc
     1801 cggtttcggc gcgcaagagc aggaaatcat aggcggcacg gaaacgcgcc tgtgcaaaca
     1861 gtttgtgcgg ccttgcgcct ttgcggtttt caaactgcgg ctggaacatc caaatttcgc
     1921 gcatcgtggc ggaaaagcgt tgcggtacgc cccaaccgcg ttcgacggtt tcgcgcatcg
     1981 tattgattgc atcggacagg gcgggcacgg gtttcaaacc ctgttgcaga ttgcttttcc
     2041 aatggcgttc caactcgggc cacatcaggg ccgccagtac gaaaccgacc gaaaccgatt
     2101 tgtcggcacg cagccgctca tcggtatttt tcagggcaag caccatcatt tttccggcga
     2161 tgccgtctga aacgcgcaag gcattgagca gcggatggat gtcgtccggt atgtcaaatc
     2221 cgttcaaacg ttttagacac tcgcgcgcgt gccctgaaaa cagcagtttc ataatttcgt
     2281 cgaacagccg cgctaccggt tcgtgcttca gacggcatat cgattcggca atcggtgcgg
     2341 cggtttcttc cgacagctca aagcccaatt tgcccgacag gcggatggcg cgcaaaatcc
     2401 tgacagggtc ttcctgatag cgttcggcgg catcgccaat cataaccagc ctgcgggcgg
     2461 caacatcggc aatcccgttg tggaaatcca aaatctcttc tttttccgga tcgtaataca
     2521 aggcattgca ggtaaaatcg cgccgcatcg catcttcttc gatgctgccg taggtgttgt
     2581 ccttcataat cctgccgtgc gcgttctgat gaactttcgc accgccgcga aacgtcgtta
     2641 cttcgataat ctccgcaccg ttcatcacat ggacgatttg aaaacgcctg ccgacgatgc
     2701 ggctgcggcg gaagagtttg tgcacctgtt cgggcatggc atcggttgcg acatcgaaat
     2761 ccttgggttc gatgccgagc agcaggtccc tgatcgcgcc gccgaccaca taagcctgaa
     2821 accccgcccc tttcagacgg cgtatgacgt tttcggcggc aaagctcaac atttcggcac
     2881 ggatgttgtg tctttcggca ggaatgaccg ttttactttc cgctttttta ctgctccgac
     2941 cggaaggcag catcttattc aaccattttt tcagcataaa tattctaacc cgaagagcgg
     3001 catctccacg ggcaaccgtg ttcagacggc atcaggcggt acgattccgc aagatgccgt
     3061 aaccataaac aaaaggcgta gaaactacgc cgtccgaatc acgccgtcct cgcggcggcg
     3121 gcaacgcgcc attataacag attgcccttt aaaaaaatca gaccctgctt ttgtggcgg
//
LOCUS       DAWFJW010000158         3173 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFJW010000158 DAWFJW010000000
VERSION     DAWFJW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.158"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..579
                     /locus_tag="WGR19_001965"
     CDS             <1..579
                     /locus_tag="WGR19_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0799425.1"
                     /translation="DATIIQTAGSTQRQAIEVDEEGQVGGQTTPSKDKDARWTKKNGL
                     YKLGYKQHTRTDAEGYIEKLHITPANAHECNHLLPLLEGIAEGTTVYADKGYDSAENR
                     QHLEERQLLDGIMRKAHRNRPLTEVQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAY
                     FGLLKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            684..1241
                     /locus_tag="WGR19_001966"
     CDS             684..1241
                     /locus_tag="WGR19_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0799426.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAVADIARTYSAHY
                     LNPNNRNMTLFPDALPCLDKLKAQGFRLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGLCGELGFDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLSAPHLAVLDGLSELPGFLAQHYA"
     gene            complement(1328..2296)
                     /gene="ispH"
                     /locus_tag="WGR19_001967"
                     /gene_synonym="lytB"
     CDS             complement(1328..2296)
                     /gene="ispH"
                     /locus_tag="WGR19_001967"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0799427.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGEEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(2325..2822)
                     /locus_tag="WGR19_001968"
     CDS             complement(2325..2822)
                     /locus_tag="WGR19_001968"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0799428.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          758 a          931 c          799 g          685 t
ORIGIN      
        1 gacgccacca ttattcagac tgccggcagc acacagcgtc aggctataga agtcgatgaa
       61 gaaggacaag tcggcggcca aaccacaccg agtaaggata aagatgcccg ttggacaaag
      121 aaaaacggcc tctacaaact cggttacaaa caacataccc gtaccgatgc agaaggctat
      181 atcgagaaac tgcacatcac ccccgccaat gcccatgagt gcaaccacct gttgcctttg
      241 ctggaaggta tagccgaagg cacgaccgtc tatgccgaca aaggctatga cagtgcggaa
      301 aaccggcaac atctggaaga gcgtcagttg ttagacggca ttatgcgcaa agcccaccgc
      361 aaccgtccgc tgacggaagt gcaaaccaaa cgcaaccggt atttatcgaa gacccgttat
      421 gtggtcgaac aaagcttcgg tacgctgcac cgtaaattcc gctatgcgcg ggcagcctat
      481 ttcgggctgc tcaaagtgag tgcgcaaagc catctgaaag cgatgtgttt gaacctgttg
      541 aaagccgcca acaggctaag tgcgcccgct gccgcctaaa aggcagcacg gatgcctgat
      601 tatcgggtat ccggggagga ttaagggggt atttgggtag aattagggag tgattggtag
      661 cggaaataga cgaaaacctg tgtttgggtt tcggctgtcg ggagggaaag gaattttgca
      721 aaggtctcag cctgcccgaa atcatccgcg acctgctcga aatgccgtct gaaaccgccg
      781 ttgccgacat cgcacgcacc tattccgcgc attacctcaa tcccaacaac cgcaatatga
      841 cgctctttcc cgatgccctg ccctgtctgg acaagctcaa agcacaaggc ttccggcttg
      901 ccgtcgccac gggcaaaggg cgggcgggtt tggacaacgc catcagccaa accgccaccg
      961 gcggctattg gctcgccacc gcctgcgcgg gggaatatcc ctccaaaccc tcgcccgaaa
     1021 tggtattcgg actctgcggc gaactggggt tcgacccgaa agaggcattg gtcgtcggcg
     1081 atacggcgca cgacctgcat atggcggcaa acgcaggcgc ggcggcagtc ggcgtggcca
     1141 ccggcgcaca ttcgcgcgaa cagctcctta gcgcaccgca tctcgccgta ttggacggtt
     1201 tgtccgaact gcccggtttt cttgcacagc attacgcctg attggtttcc gcatccggca
     1261 cgcggcaaaa atgccgtctg aagcctgttc agacggcatt tgtgttgccc aaacattcaa
     1321 cgcttgctca acgtttgcac aaatcgggtt tggtttcgcc ctcgcggcgc agctctttgg
     1381 gcaggacgaa cacaatgctt tcttcctcac cctcgccttc gcgtacggtt tcgtgccccc
     1441 atccgcgtat ggttgccagt acttcccgca ccaacacttc gggcgcggac gcgcctgccg
     1501 ttacgccgac tttgtttttg ccctcaaacc atgcgcgttg caggtagcct gcattatcca
     1561 ccatatacgc atcgattccg cgcgatgccg ccacttcgcg caagcggttg ctgttggacg
     1621 aattgggcga accgaccaca atcacgatgt cgcactgttc tgccaactct ttgacggcgg
     1681 tttgccggtt ggtcgtcgca tagcagatat cttccttgtg cggattgcgg atattgggga
     1741 aacgcgcgtt cagcgcggcg atgatgtctt tggtttcatc gaccgagagc gtggtttggc
     1801 tgacataggc gagtttgtcg gggtttctga cttcgagttt tgccacatct ccgaccgttt
     1861 cgaccaaaag cattttgccc ggcgcaagct gccccatcgt tccttcaacc tcgacgtgcc
     1921 ccttatgccc gatcatgatg atttcacagt cttgggcatc cagtcgggcg acttccttat
     1981 gcactttcgt caccagcggg caagtcgcat caaacacgcg gaaaccgcgc tccgccgctt
     2041 cttgccgcac cgccttcgat acgccgtgtg ccgaataaac cagtgtcgcg cccggcggca
     2101 cttccgccaa gtcttcaata aacaccgcac ctttttcacg caggttgtcc acgacgaatt
     2161 tgttgtgaac gacttcgtgg cgcacataaa tcggcgcgcc gaactcttcc aaagcacgtt
     2221 cgacaatact gattgcccga tccacaccag cgcagaagcc gcgcggattg gcaaggatga
     2281 tggttttctc gttcataagc ccggtatttc gttttcagac ggcatcaata tttttcttct
     2341 tgggttttac ggtggacgat gttgtccaac accgccaaca ccgcaccgac gcagataaag
     2401 ctgtcggcaa tattaaaggc gggataaaac caattttgcc aataaaacaa taagaaatcg
     2461 acgacatgac cgtgtatcag gcggtcgatg acattgccta acgcaccgcc gataatcatt
     2521 gccgcacccg ttttgccgag ggttgcaaac tcatcgcgca agatggcgcg taccaaatac
     2581 gcgctcaccg ccaccgccag caccaaaaaa aagtattttt gccagccgcc ctgatcggca
     2641 aggaagctga acgccgcacc cgggttgtac accagcgtca gatcgaaaaa ggaaggaatg
     2701 acattgacgc gttcccgata ctgaaacgac gacagcaccg cccacttcga ccactggtcc
     2761 agcacgatgg cggcaagtgc caatacccaa tagcgcgttt tacttgaaac agatgaagac
     2821 atatttttca acagccggta aaagagtacc attttacccg aaaaccccct ttcctgtacc
     2881 cgaaacggca aatgccgtaa tcttaaaacc cgtcattccc gacaacaccg taatctcgaa
     2941 acccgtcatt cccgcgtagg cgggaatcca gacctgtccg cacagaaact tatcggataa
     3001 aaacagttgc ccaaaccccg cgttctatag tggattaaat tcaaaccagt acggcattgc
     3061 ctcgccttag ctcaaagaga acgattctct aaggtgctga agcaccgagt gaatcggttc
     3121 cgtactattt gtactgtctg cggcttcgtt gccttgtcct gatttaaatt taa
//
LOCUS       DAWFJW010000159         3172 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFJW010000159 DAWFJW010000000
VERSION     DAWFJW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.159"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(205..1056)
                     /locus_tag="WGR19_001969"
     CDS             complement(205..1056)
                     /locus_tag="WGR19_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0799429.1"
                     /translation="MTLLGYKLKQTQQLDRRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEEDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1404..2918
                     /gene="katA"
                     /locus_tag="WGR19_001970"
     CDS             1404..2918
                     /gene="katA"
                     /locus_tag="WGR19_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0799430.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGDFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
BASE COUNT          752 a          905 c          822 g          693 t
ORIGIN      
        1 aaaccctcgt cattcccgcg aaagcgggaa tccaggttcg ttgagtttcg gtcatttccg
       61 ataaattctt gcagctttgc gtttctagat tccctctttc gcgggaatga cgggatgcag
      121 gtttccgcgc ggacggattc ggcattcccg ctttcgcaga aatgccggct tttggtttgt
      181 tgtctttata agattattcg gcaattattc tgcggttttg cgtttgtgtg ctgccggaat
      241 ctcaaaggat tctctgtatt tggcaatggt gcggcgagaa acctccatgc cgcggaaagc
      301 cagcaggttg gcgagcgcct cgtcagaata cggcgtgggt gtggacgagg caaaaccggc
      361 ggcgggaaag ggattgagcg aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc
      421 ggtttggggc aggcgttttt ttattcgcgc gagggtttgg ctgcggttgc cgtcaatgct
      481 gtcgagggcg tttcggacga tatgcagggc ggagggtttg gcagcacatt cgcccgatct
      541 ttctatctgc agtatcagcg attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa
      601 ttttttcaat gcggtcaggg cttgtttcag cattgcttca tccaacatcc actccaaggg
      661 cgtatggtcg aggatgtctt cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc
      721 gatgaggatg tggacacagg cggattcttg gtcggaaagc gggtgttcgc acacttgcgc
      781 gtgcaggtat tgcttgaaat cttcttcgcc ggcgatgttg gacagcatat cttcgccttc
      841 gtctccgccg atttggcgcg cgggtgcggt gtaatggctg aattcggcat cggaaaattc
      901 atccgtgtct ttgcgttcga gcagggggtt gtccgacagc cagttttcga cctcgcgttc
      961 aagttcgata cccgacatct gcaatacgcg caaagattgt tgcaaccgcc ggtcgagctg
     1021 ctgggtctgt ttgagcttat atccgagtaa ggtcatgata atgtggggga aattattatt
     1081 ttcagcctgc cggcgcaaaa aatgccgcaa agcgtcattg cattataaat ggttttaacg
     1141 ggcggattcg ggttcttcgt gataacaaaa aacaaacgga aatcaaaaac cgattgctta
     1201 taacaatatt aaatcgattt catagtttta atagcgaaaa tcttggcgta tagtcgcacc
     1261 catagttttt gcaaaaggga aacaaaatgt cgattcaaga aatttattgc aatcaagaaa
     1321 ccggttacga atacgctttc cgccaaatcg tactgtaagc cgtttacccc catttgacca
     1381 acccgaaaaa aggaacaaga gcgatgacta cctccaaatg ccccgtaacc catctgacca
     1441 tgaacaacgg cgcgcctgtt gccgacaatc aaaacagcct gaccgccggt cctcgtggcc
     1501 cactgctggc gcaggatttg tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca
     1561 ttcccgaacg ccgtatgcac gccaaaggtt cgggcgcgtt cggtacgttt accgtaacgc
     1621 acgacatcac caaatacacc cgcgccaaaa ttttcagcga agtcggcaaa aaaaccgaga
     1681 tgttcgcccg tttcaccacc gtggcaggcg aacgcggcgc agccgatgcg gaacgcgaca
     1741 tccgcggctt tgccttgaag ttctataccg aagaaggcaa ctgggatgtg gtcggcaaca
     1801 acacgcctgt gttcttcctc cgcgacccgc gtaaattccc cgacctgaat aaagccgtca
     1861 aacgcgatcc gcgcaccaat atgcgttccg ccacaaacaa ctgggacttc tggacgctgc
     1921 tgcccgaagc gctgcaccaa gttaccatcg tcatgagcga ccgcggcatt cccgccggct
     1981 accgccatat gcacggcttc ggttcgcata cctacagctt ttggaacgaa gcgggcgaac
     2041 gcttttgggt aaaattccat ttccgcaccc aacaaggcat taaaaacctg accaacgaag
     2101 aagccgccaa aatcatcgcc gacgaccgcg aaagccacca gcgcgacctg tacgaagcca
     2161 tcgagcgcgg cgacttcccg aaatggacga tgtacatcca agtcatgcct gaagcagacg
     2221 cggaaaaagt accttatcat ccgtttgacc tgaccaaagt ttggccgaaa aaagactatc
     2281 cgctgattga agtgggcgaa ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg
     2341 tggaacaatc cgccttcgca ccgagcaacc tcgttcccgg tgtcggcgcc agcccagata
     2401 aaatgttgca ggcgcgtttg ttcaattacg ccgacgcgca acgctaccgc ttgggcgtga
     2461 acttccgcca aatccctgtc aaccgtccgc gttgccctgt tcacagcaac cagcgcgacg
     2521 gacaaggacg cgccgacggc aactacggca gcctgccgca ctacgaaccc aacagcttcg
     2581 gccaatggca gcagcagccc gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg
     2641 cgcactggga ctaccgccaa gacgatgacg actatttcag ccaaccgcgc gccctgttca
     2701 acctgatgaa cgacgcgcag aaacaggcat tgttcggcaa caccgccgcc gcaatgggcg
     2761 acgcgcccga cttcatcaaa taccgccata tccgcaactg ctaccgttgc gatccggcat
     2821 acggcgaagg cgtggcaaaa gccctcggac tgactgtcga agacgcacaa gccgcccgcg
     2881 cgaccgaccc cgcactcggc cagcccggtt tgctgtaagg gggcattatg tggatggaaa
     2941 ttgaagaaat cctgtcccgc gccgtccgct accggaaata accgggcata aaaatgccgt
     3001 ctgaaacgtc gtttgaccgc ttcagacggc atttccacac cccacccccg ccgtttcagt
     3061 gggcgttttt tattaaacgc aaaatatccc gtcattcccg ccccacagcc cacttttccg
     3121 ccgtcattcc cgcaccatcc ccaccggttc aaaccggcac gaaaactttt cc
//
LOCUS       DAWFJW010000160         3124 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFJW010000160 DAWFJW010000000
VERSION     DAWFJW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.160"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            901..3060
                     /locus_tag="WGR19_001971"
     CDS             901..3060
                     /locus_tag="WGR19_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0799431.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTSFGGDDHRIVLSHMKDQHRGIRTVREEFTVTNNSRLTLARQ
                     APAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNHT
                     RITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFSIPTTEKKNGQDVAKP
                     ADQQAKDRKDEALVHSYRLTNPTKTDTGAYIEAIHEINGFTLTGGLRYDRFKVKTHDG
                     KTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKA
                     ERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNHGY
                     ELGASYRTGGLTAKVGVSHSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFQNP
                     NLEIGWRGRYVQKAVGSILVSGQKNREGKLENVVRQGFGVNDVFANWKPLGKDTLNVN
                     LSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          809 a          911 c          709 g          695 t
ORIGIN      
        1 cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctcaagca ccaagtgaat
       61 cggttccgtc ctatccgtac tgtctgcggc ttcgccgcct tgtcctgatt tccgttcgtt
      121 ttcggttatt cccgataaat taccgccgtt tctcatcatt tctttaaccc ttcgtcattc
      181 ccgcgcaggc gggaatctag tttttttgag ttccagttgt ttctgataaa ttcttgcagc
      241 tttgagttcc tagattccca cttttgcggg aaggacgcgg aaaggttgct tttcgtcatt
      301 cccgcgcagg cgggaatcca gtctgttcgg tttcggtttt ttccgataga ttcctgccgc
      361 gttaggggtt ctagattccc gctttcgcgg gaaggacgcg gaaaggttgc ttttcgtcat
      421 tcccgcgcag gcctcgtcat tcccgcacag gcttcgtcat tcccgcgcag gcgggaatcc
      481 agtctgttcg gtttcagtca tttccgatag attcctgccg cgttaggggt tctagattcc
      541 cgctttcgcg ggaatgacgc ggaaaggttg cttttcgtca ttcccgcatt tgttaatcca
      601 ctatatttcc gccgtttttt atagtggact aacaaaaacc agtacagcgt tgcctcgcct
      661 tgccgtacta tctgtactgt ctgcggcttc gtcgccttat cctgattttt gttagtccac
      721 tatacatttc cgacaaaacc tgtcaacaaa aaacaacact tcgcaaataa aaacgataat
      781 cagctttaca caaatccccc ctctcgttaa tataaacaaa aataattatt atttttctta
      841 tcctgccaaa ccttaacggt ttggcttaac ttcccttcat atactcaaga ggacgattga
      901 atgaataccc cattgttccg tctcagcctg ctctcgctta ccctggcggc aggttttgcc
      961 cacgcggcag aaaataatgc caaggtcgta ctggataccg ttactgtaaa aggcgaccgc
     1021 caaggcagca aaatccgtac caacatcgtt acgcttcaac aaaaagacga aagcaccgca
     1081 accgatatgc gcgaactctt aaaagaagag ccctccatcg atttcggcgg cggcaacggc
     1141 acgtcccaat tcctgacgct gcgcggcatg ggtcagaact ctgtcgacat caaggtggac
     1201 aacgcctatt ccgacagcca aatcctttac caccaaggca gatttattgt cgatcccgct
     1261 ttggttaaag tcgtttccgt acaaaaaggc gcgggttccg cctctgccgg tatcggcgcg
     1321 accaacggcg cgattatcgc caaaaccgtc gatgcccaag acctgctcaa aggcttggat
     1381 aaaaactggg gcgtgcgcct caacagcggc tttgccagca acgaaggcgt aagctacggc
     1441 gcaagcgtat tcggaaaaga gggcaacttc gacggcttgt tctcttacaa ccgcaacgat
     1501 gaaaaagatt acgaagccgg caaaggtttc cgcaatgtca acggcggcaa aaccgtaccg
     1561 tacagcgcgc tggacaaacg cagctacctc gccaaaatcg gaacaagctt cggcggcgac
     1621 gaccaccgca tcgtgttgag ccatatgaaa gaccaacacc ggggcatccg cactgtgcgt
     1681 gaagaattta ccgtcactaa taactcacgt ctgaccttag cacgccaagc acctgcttac
     1741 cgcgaaacca ctcaatccaa caccaacttg gcgtacaccg gcaaagattt gggctttgtc
     1801 gaaaaactgg atgccaacgc ctatgtgttg gaaaagaaac gctattccgc cgatgacaaa
     1861 gataacggct acgcaggcaa tgtaaaaggc cccaaccata cccgaatcac cactcggggt
     1921 atgaacttca acttcgacag ccgccttgcc gaacaaaccc tgttgaaata cggtatcaac
     1981 taccgccatc aggaaatcaa accgcaagcg tttttgaact cgaaattctc catcccgacg
     2041 acagaaaaga aaaacggtca agatgttgct aaaccagcag accaacaagc caaagaccgc
     2101 aaagacgaag cccttgtcca ttcctaccgt ctgaccaacc cgaccaaaac cgataccggc
     2161 gcgtatatcg aagccattca cgaaattaac ggctttaccc tgaccggcgg gctgcgttac
     2221 gaccgcttca aggtgaaaac ccacgacggc aaaaccgttt caagcagcaa ccttaacccg
     2281 agtttcggcg tgatttggca gccgcacgaa cactggagct tcagcgcgag ccacaactac
     2341 gccagccgta gcccgcgcct gtatgacgcg ctgcaaaccc acggcaaacg cggcatcatc
     2401 tcgattgccg acggcacgaa agccgaacgc gcgcgcaata ccgaaatcgg cttcaactac
     2461 aacgacggca cgtttgccgc aaacggcagc tacttctggc agaccatcaa agacgcgctt
     2521 gccaatccgc aaaaccgcca cgtctctgcc gccgtccgtg aagccgtcaa tgccggttac
     2581 atcaaaaacc acggttacga attgggcgcg tcctaccgca ccggcggcct gactgccaaa
     2641 gtcggcgtaa gccacagcaa accgcgcttt tacgataccc atcctaaaaa actgttgagc
     2701 gcgaaccccg agtttggcgc acaaaccggc cgcacttgga cggcttccct tgcctaccgc
     2761 ttccaaaatc cgaatctgga aatcggctgg cgcggacgct atgttcaaaa agccgtgggt
     2821 tcgatattgg tgtcaggtca aaaaaaccgc gagggcaaat tggaaaacgt tgtacgccaa
     2881 ggtttcggtg tgaacgatgt cttcgccaac tggaaaccgc tgggcaaaga cacgctcaat
     2941 gtcaatcttt cagtcaacaa cgtgttcaac acgttctact atccgcacag ccaacgctgg
     3001 accaataccc tgccgggcgt gggacgtgat gtacgcttgg gcgtgaacta caagttctaa
     3061 aacgcacatc ccgaaaaaat gccgtctgaa agcctttcag acggcatctg tcctgataat
     3121 ttga
//
LOCUS       DAWFJW010000161         3120 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFJW010000161 DAWFJW010000000
VERSION     DAWFJW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.161"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            33..1859
                     /gene="mafB"
                     /locus_tag="WGR19_001972"
     CDS             33..1859
                     /gene="mafB"
                     /locus_tag="WGR19_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0799432.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            1862..2326
                     /locus_tag="WGR19_001973"
     CDS             1862..2326
                     /locus_tag="WGR19_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799433.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            2515..2718
                     /locus_tag="WGR19_001974"
                     /pseudo
     CDS             2515..2718
                     /locus_tag="WGR19_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2709..2996
                     /locus_tag="WGR19_001975"
     CDS             2709..2996
                     /locus_tag="WGR19_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ0799434.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNKLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
BASE COUNT         1021 a          641 c          684 g          774 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 attcttttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggaa cttgccgagc gcagcggcca tatcggattg ggaaaaatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgatgaa gccggtagtc ccgttgacgg atttagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgccg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg tagtatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcgggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgttaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gcataagcga aggctcaaac attgctgtca tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tatggcagcc atccccatca aagggattgg agctgttcgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctatca agcggtcgca gatgggcgcg atcgcattgc
     1081 cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gcaaaaatgt caaactggca gaccaacgcc
     1261 acccgaagac aggcgtaccg tttgacggta aagggtttcc gaattttgag aagcacgtga
     1321 aatatgatac gaagctcgat attcaagaat tatcgggggg cggtatacct aaggctaagc
     1381 ctgtgtttga tgcgaaaccg agatgggagg ttgataggaa gcttaataaa ttgacaactc
     1441 gtgagcaggt cgagaaaaat gttcaggaaa taaggaacgg taataaaaac agtaacttta
     1501 gccaacatgc tcaactagag agggaaatta ataaactaaa atctgccgat gaaattaatt
     1561 ttgcagatgg aatgggaaaa tttaccgata gcatgaatga caaggctttt agtaggcttg
     1621 tgaaatcagt taaagagaat ggcttcacaa atccagttgt ggagtacgtt gaaataaatg
     1681 gaaaagcata tatcgtaaga ggaaataata gggtttttgc tgcagaatac cttggcagga
     1741 tacatgaatt aaaatttaaa aaagttgact ttcctgttcc taatactagt tggaaaaatc
     1801 ctactgatgt cttgaatgaa tcaggtaatg ttaagagacc tcgttatagg agtaaataaa
     1861 aatggcaatt tggaattatc ggtactacct tatcccatca gctgctatca gaaataaatt
     1921 taatgcaaat aaagatatca tacttgatga gtatcgatct aatggttttc agaattttaa
     1981 tgagaataaa agttttgaaa attactttat cgataatgat gttatattat tatcaataat
     2041 aaatgaagca aaaaaacagc ttaaattaaa agagtcttgg gataaagacg caatcatgtt
     2101 ttgtgataat tttggtaata gtcttaccgt ttggccagat gatatagagt gcgaacttga
     2161 tttaagattt gattatacta aatttattca gaaaaccatt gattgggcaa taaaatataa
     2221 ttgtctactt gtaatagaaa aaacaggaaa tgtagtttcc cctaatataa ataatctgat
     2281 gtatgaaata aaagcatatt tggaaagcaa gccgtggccc atatgaaacc taaactcaac
     2341 aagtaggatg tgtgcggaac gcacgtatgc ggttctcaag gtttgagcta agaggccgtc
     2401 tgaaaacaga aaaactgttt cagacgacct ttcttttaac cagttgccac agcaaccgga
     2461 caaaagcagc ctacctccac atccatatag gcaatacagg ggagatattt tgtaaattct
     2521 acgaatattt tacctgctaa acagggtagg atatggtatg aagcgaacat tggcttaata
     2581 aacactatgt caagatcgaa tcaggctggt actagattgt tgtattccaa tgatggattg
     2641 ctatatataa caactgatca ttatatctct gcaacacggt ttggagcttg gaaatgggaa
     2701 tataacttat gcaattagag attatcggta gtaaaattta tacggaacaa gattttcata
     2761 atcaaatttc aaaaatattt tctatacaag attattatgg gaacaatctt gatgctctgt
     2821 gggatttatt aagcacaaat gtagaacgac cgattacttt ggtatggaaa gatgctatgt
     2881 tctcaaaaaa taaattagaa aatatattta ttgaaatcgt aaatgttcta gaaagagtta
     2941 agaaacaaga tgaggattat ggattcgaag aaaaatttaa ttatatttta gagtaagtaa
     3001 accctaatta cattacgtac acaggcttaa aactccccag agccaattaa gcaagccgta
     3061 acccatataa aacttaaact caacaagtag catgtgtgcg gaacgtacgc atgcggttct
//
LOCUS       DAWFJW010000162         3113 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFJW010000162 DAWFJW010000000
VERSION     DAWFJW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.162"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..480
                     /locus_tag="WGR19_001976"
     CDS             <1..480
                     /locus_tag="WGR19_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799435.1"
                     /translation="AAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRK
                     GAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVL
                     PDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            499..873
                     /locus_tag="WGR19_001977"
     CDS             499..873
                     /locus_tag="WGR19_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0799436.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            1258..2688
                     /locus_tag="WGR19_001978"
                     /pseudo
     CDS             1258..2688
                     /locus_tag="WGR19_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            2707..2970
                     /locus_tag="WGR19_001979"
     CDS             2707..2970
                     /locus_tag="WGR19_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799437.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
BASE COUNT          964 a          610 c          739 g          800 t
ORIGIN      
        1 gctgctccta aagaaacaat aaacaatttg gcaaatttag ccaaagcaga acagcagatt
       61 ttattccgta ttgcccaacg cgatacgcaa ctggatgcat ggaagacggg atttaacaat
      121 agagtaagga aaggagcagg cttgcttgat gcaagtaata ttccgataac cattaacgga
      181 aaaaccatca aacctgtaca agccataagc ttaaagggag cacccgttta cagcggcgta
      241 agcgaacagg agatttttgc gctttatcgg cagatgactg gccagaatcc gaattttaga
      301 gttttgcctg acggaagatt agcaaatggc attatcagta ctggagaatg ggcaggaaca
      361 aaaattgcat taagaaattt ttcaaaaaca gagaattcaa ctcaagcacg atggacatta
      421 gatttgcaga atcctccatc atttattaaa ggtactaaat tggagcttaa attccaataa
      481 tttacaaagg attttaccgt ggatgagaaa caaaaaatta agattcttga ttttcaaatc
      541 gatttatcct caatttttaa ctcttataaa aatcaaatgg gtattaatat tcaagataaa
      601 aacttaaaaa acaatttctg ttcttttatg gaagaactgt taaatgacgg ttcaatccgt
      661 ttacatgatt ataccgacgg tatcggaatt cctctaactg gaacttcaaa agaacaagtg
      721 cagaaattga aagacatatg gcctactttg gaagatgccc aagcaatatg gcctgaagac
      781 ccttggtatt acttagaatg gctttggtgg gatattgcgt gtccaataga tttggccgat
      841 ttgccgaata ttgatattta tgagcaagcg taggtatggt tagccgcctt tagcggcgta
      901 accgtacgca tatcagcaaa ctttataaaa taacaaggcc gtctgaaatc tgtttttcaa
      961 ctttttcaga cggccttgca acttggcatt tcattcgtac ggttacgccg ctaaaggcgg
     1021 ctaaccgtac ctacgagctc tgataaaaat gatttatgga agcaagctgt agcctgcatg
     1081 aaacctaaaa tccatgcgta aggtgtgtgc ttcagcgcgc acgcgttcca tgatttacgg
     1141 ctcaatgccg tctgaaaagc tcaatttttt cagacggcat ttgttatgca agtaaatatt
     1201 cagattccct atatactgcc cagatgcgtg cgtgctgaag acacccccta cgcttgctat
     1261 ttgaaacagc tccaagtcac caaagacgtc aactggaacc aggtacaact ggcgtacgac
     1321 aaatgggact ataaacagga gggcttaacc gaagcaggtg cggcgattat cgcactggcc
     1381 gttaccgtgg tcacctcagg cgcgggagcc ggagccgcac tgggcttaaa cggcgcggcc
     1441 gcagcggcaa ccgatgccgc attcgcctcg ctggccagcc aggcttccgt atcgctcatc
     1501 aacaataaag gcgatgtcgg taaaaccctg aaggagctgg gcagaagccg cacagtgaaa
     1561 aaactggttg tagcagcggc catcgcaggc gtatccaaca aaatcggtgc ttcctccctt
     1621 gccacttgga gcgaaacccc ttgggtaaac aacctcaacg tcaacttggc taatgcgggc
     1681 agtgccgcgc tgattaacac cgccgttaac ggcggcagtc tgaaagacaa tctggaagcg
     1741 aatatccttg cggctttggt gaatactgcg catggagagg cagccagtaa aatcaaacag
     1801 ttggatcagc actacattgc ccataagatt gcccatgcca tagcgggctg tgcggctgcg
     1861 gcggcgaata agggcaagtg tcaggacggc gcgattggtg cggtcgtggg ggagattgtc
     1921 ggggaggctt tggttaagaa taccgatttc agcggtatga ctgcttctga aattgaaaaa
     1981 gctaaagcga atattactgc gtatgcaaaa ttggtagccg gagcgactgt aggtgttaca
     2041 ggaggcaatg ttgatgtggc ggcaaatgct tccgaaacag ctgttaaaaa taatgcatta
     2101 gatattattt gggatatcgg caacctcgta tgggacggcg gtaaatggat ttacgccaaa
     2161 tctattggcg ataagcagat ggctcgagaa gcggcgattg attttggtgt ggatgccgcc
     2221 gcagctgccg ttccctttgt tccggcaggt gcgactaaaa tcagccgagg cggggcttat
     2281 gttctgaagg cgggagacga agcagttgat acggctaaag ccatacagga aattcagaag
     2341 cagaccggaa tcaagcttac ttatgataag gttaataagg tttggacaac accggcgggg
     2401 ttagattatg ggttagatgc taagcatggt aataggatta aacatgtttt agcccataca
     2461 attccaaatc caaacaaacc tgttcattct gtttttaatg tgtcccgtaa agaagttttg
     2521 cctttggttg atgaagcttg gagaatgaaa ggaaatcctt tgccaaatga ttcatccgta
     2581 tatcttgtag atatgaagaa acctattgga acaaaaggag aaacaaaagt gcggattgtt
     2641 gtgcaaaaag gaacaaataa aatcatttct gcatatcctc agaaataatt aagaaaggaa
     2701 tctcttatgg ataaagaaat taaaatttgc ccaagatgtg agcaaggcta cctttatcat
     2761 gcaaagccta aatatttctc tggggaggtc attttatgcg atgaatgtta tgctatgtgg
     2821 cttggggata tgaaaatttt ttacggacaa tatggaaaag atttttatga ttatcatgag
     2881 tttatgaaag ataaaggcat agaagaaata aatatgtggg aaggagagct ttttgatcac
     2941 ccatattatg aggatgaaaa atttaaataa ttgattttct gttccccgaa tttgggaaat
     3001 acgatgatat tttaaaccca aatattattt aaagtagcaa taggccgtct gaatatccgt
     3061 ttttcagacg gcctcaatgc aactgctggc agccgaaggc attcaccaac acc
//
LOCUS       DAWFJW010000163         3068 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFJW010000163 DAWFJW010000000
VERSION     DAWFJW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.163"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(261..737)
                     /gene="greA"
                     /locus_tag="WGR19_001980"
     CDS             complement(261..737)
                     /gene="greA"
                     /locus_tag="WGR19_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0799438.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1129..2430
                     /gene="aroA"
                     /locus_tag="WGR19_001981"
     CDS             1129..2430
                     /gene="aroA"
                     /locus_tag="WGR19_001981"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0799439.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGIDVEYLGNEHYPPLHIGERQDNGE
                     HVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EDYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2473..3006)
                     /locus_tag="WGR19_001982"
     CDS             complement(2473..3006)
                     /locus_tag="WGR19_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0799440.1"
                     /translation="MFPPDKTLFLCLSALFLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTAIGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIRTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          649 a          835 c          825 g          759 t
ORIGIN      
        1 caggttttct taaccctgcg tcctagattc ccgcttttgc gggaatgacg gcgacagggt
       61 tgctgttata gtggatgaac aaaaaccggt acggcgttgc ctcgccttag ctcaaagaga
      121 acgattctct aaggtgctca agcaccaagt gaatcgattc cgtactatct gtactgtctt
      181 cggcttcgtc gccttgtcct gatttttatt aatccactat aatttcctgc gtgtgtcggg
      241 tgtatcgaaa tcaagccgaa tcaaatatat cggacttcga taatgtcgta ttcgcgcacg
      301 ccgcccgggg cttggacttc cgccgtatcg ccctcttcct tgccgattaa ggcgcgggca
      361 atcggtgagc cgacatagat tttgccctgt ttgatgtcgg cttcgtcttc gccgacaatt
      421 tgataggtaa cgtgttcttc cgtttccaaa tcttccagcg taaccgtcgt accgaacacg
      481 attttgcctt cggcgtggat ttcggtcgga ttgatgatgt gggcaacgga aagtttgtgt
      541 tccagctcgg aaatgcggcc ctcgataaag ccttggcgtt ctttggcggc ttcgtattcg
      601 gcgttttcgg acaaatcgcc gtgcgaacgg gcttcggcaa tcgcttcgat tacttcggga
      661 cgcgccacgc ttttgagctg ctgcaattcc tgtttcagca attccgcacc gcgtacggtc
      721 agggggattt tttgcatcgg tcttttttct ccatattccg gcacaccgtt ttgcggcagc
      781 aagcataccg cgtaccgtct tgttttgtgc gtccggatat taaaataaaa atacaagccg
      841 cccggaaaat cggcggcttg tctgtcgttg aacagcggct attctaccaa attctatgaa
      901 attggcaatc gtgccgcgcc gccggcaaac gcgccatgtc cgcaacaaaa gctgaaaata
      961 tgccgacaaa gaaattttag aaacaaaaaa tttaaaaata atcaattttc ggcataaaaa
     1021 accacattta cggactttaa aaccgaaaat gccaaacctg agatttttca tacggcattt
     1081 gcaccagtat aatgcaggct gtttttatct ttatatttac gttttgccat gaccgaatcc
     1141 gtccgcctcc ccgtcgcccg tctcaaacct tccaccgtcg ccctgcccgg ctccaaaagc
     1201 atcagcaacc gcaccctgct gcttgccgcc ttgtccgaca atgcttgcga aatccattcc
     1261 ctgctcaaat ccgacgatac cgaccgtatg ctcgaagcac tcgataaact cggcgttcaa
     1321 atcgaatatc ttgccgaaga ccgtctgaaa gtgcacggca caggcggacg cttccccaac
     1381 cgcactgccg atttgttttt gggcaacgcg ggcacggcgt tccgcccgtt aaccgccgct
     1441 ctggccgttt tgggcggcga ttatcatctg cacggcgtgc ctcgtatgca cgaacgtcct
     1501 atcggcgatt tggtcgatgc gttgcggatt gccggcatcg atgtcgaata tctcggcaac
     1561 gaacactatc cgccgcttca tatcggcgaa cgccaagaca acggcgaaca cgtgattccg
     1621 attaaaggca atgtgtccag ccagtttctg accgcccttt taatggcgtt gccgctgacc
     1681 ggacaggcat ttgaaatccg tatggtcggc gagttgattt ccaagcccta tatcgacatt
     1741 actttaaaac tgatggcgca attcggcgta caggttgcca atgaagacta ccgcgtcttc
     1801 aaaattcccg ccgatgcgca ctaccacgcg cccgaacact tgcacgtcga aggcgatgcc
     1861 tccagcgcgt cctacttcct cgcagccggt ttgattgccg ccacgcccgt gcgcgtgacc
     1921 ggcatcggcg caaacagcat acagggcgat gtcgcctttg cccgcgagct ggaaaaaatc
     1981 ggtgcggacg tggtttgggg cgaaaacttc gtcgaagttt cacgcccgaa ggaacgtgcc
     2041 gtccaatcct ttgatttgga tgcgaaccat atccccgatg ccgccatgac cctcgccatc
     2101 gtcgcgcttg ctacagggca aacctgcacg ctgcgcaaca tcggttcgtg gcgcgtcaaa
     2161 gagaccgacc gcatcgccgc aatggcaaac gagttgcgca aactcggggc aaaagtcgtc
     2221 gaagaagccg aagcaattca catcaccccg cccgaaacgc tgacacccga cgccgtcatc
     2281 gacacgtacg acgaccaccg catggcgatg tgtttctcgc tggtttcgct gttgggcgta
     2341 cccgtcgtca tcaacgatcc gaaatgcacc cacaaaacct tcccgactta tttcgacgtg
     2401 ttctcatcgc tgaccgaaac agcggaataa ggcggcattt tgccgcgatt ccggcgcggc
     2461 gcggcgggcg gctcattctg taaaaaaagt atgtgcgccg aggtagtttt tagcgtaaaa
     2521 cggtgtagaa agtttttcgg ttcggatggt tttgccgctg ctgggggcat ggatgaattc
     2581 gccgttgccg atgtagagtc cgacgtgtga gtagcggtgt gcgccgccgg tgttgaagaa
     2641 tacgaggtcg ccggccttga ggcggctgtc ggggattttg cggcttgccg ccgccatgtc
     2701 tcgggcggtg cgcggcagct tgacgttgag ggcgtttttg taaacgaatt gaatcatgcc
     2761 gctgcaatcg aagccgattg cggtgctgct gccgccccat ttgtagggcg tgccgatgag
     2821 tccgaggctg tggagcatga gttcctgcga gccttgtgtg cggtcgatgt ggctgatgcg
     2881 gacggcttgg atttgccgga ctgtctgttt gggtttcggt tggcggtgtt tgccggaggt
     2941 cgtgccgcat gaggcgagga acagtgcgct gagacagagg aaaagggttt tgtcgggggg
     3001 aaacatggtt tttcctttgc gggttcggat atccgtctga aggtgtttca gacggtatag
     3061 tggattaa
//
LOCUS       DAWFJW010000164         2998 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFJW010000164 DAWFJW010000000
VERSION     DAWFJW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.164"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>2998
                     /locus_tag="WGR19_001983"
     CDS             <1..>2998
                     /locus_tag="WGR19_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799441.1"
                     /translation="NVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQGVENSGT
                     AVSQQGTQIHSQSIQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTDTLNNQG
                     KLSQTGSQKLHIDAQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASSHSSTTTPTT
                     ATGTGTATVSISNITAPTFADGIIRTHGALDNSGSIIANGQTGVSAQQGLNNAGQIDI
                     HQLNAKGSAFDNHNGTIISDAVHIQAGSLNNQNGNITTRQQLEIETGQLDNAHGKLLS
                     AEIADLAVSGSLNNQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSDRDVAIQAKSLS
                     NNGTLAADNKLDIALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIRIKANNL
                     DNAAQGNIQSGGATDIGTQHNLTNRGLIDGQQTKIQAGQMNNIGTGRIYGDNIAIAAT
                     RLDNQDENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQATGKAQ
                     RIHNAGATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHELLREGTQ
                     HELGWSVYNDESDHLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGNDLTIDG
                     KEVFNTDSQIIAGGNLLVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHKGRDSTG
                     HSEQNYTLPEEITRNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYTSNSFTP
                     LPSSSLYIINPVNKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLGDGYYEQ
                     RLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQVAQLTS
                     DIVWLVQKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSGSLKNSG
                     TIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGILSVEQTLLLNAGNNINS
                     QSTAASSQNTQGSSTYLDRMAGIYITG"
BASE COUNT          999 a          686 c          656 g          657 t
ORIGIN      
        1 aatgtggcga ttgatgcaaa tggccgttta gtcaatagtg gcacgatggc tgccgccaat
       61 gcgaaagata cggataatac agcggaacac aaagtcaata tccgcagtca gggcgttgaa
      121 aacagcggta cggcggtatc gcaacaaggc actcaaattc acagtcagtc gattcaaaac
      181 actggcacat tattgtcctc aggcgaaata ttgattcaca attcgggcag cctgaaaaat
      241 gaaacatcag gcaccattga agccgctcgt ttggctattg ataccgacac acttaataat
      301 caaggcaaac tctctcaaac aggttcacaa aaactccata ttgatgcaca aggcaaaatg
      361 gataaccgtg gccgcatggg tttacaagat accgcaccaa ccgcgtcaaa tggttcaagc
      421 aatcaaaccg gcaatagtta caatgcatct tcccattcat ccactaccac accaacaacg
      481 gcaacaggta cgggtactgc aaccgtttct atatcaaaca taactgcgcc tacctttgct
      541 gatgggataa ttcgcactca tggtgcactg gataattcag gcagtattat tgccaatggt
      601 caaacaggtg ttagtgcgca acaaggttta aataatgcag gacaaataga cattcatcag
      661 ttaaatgcaa aaggttcggc gtttgacaat cacaatggaa caattatcag tgatgcggtc
      721 cacattcaag ccggcagcct gaataatcaa aacggcaaca tcacaacacg ccaacagtta
      781 gagattgaaa ccggtcaact ggataacgct catggcaagt tattatcagc agaaatagcg
      841 gatttagccg tttcaggcag cctgaacaat caaaatggcg aaatagcgac caatcaacaa
      901 ctgattattc acgatggtca gcaatctacc gctgtcattg ataatacgaa tggcacgata
      961 caatcagacc gtgatgttgc tattcaggca aaatcgttat ccaacaacgg cacacttgcc
     1021 gctgataata aactggatat tgcgttacaa gatgattttt atgtagaacg caatatcgtg
     1081 gcgggcaatg aattgtcgct cagtacacga ggcagcctga aaaattcaca tactttgcaa
     1141 gcaggaaaac gcattcggat taaagcaaat aaccttgata atgcagcaca aggcaacatt
     1201 caatccggcg gtgcaacaga cattggcacg cagcacaatt taaccaatag aggcttgatt
     1261 gacggacaac aaaccaaaat ccaagccggg caaatgaata atatcggtac aggtcggatt
     1321 tatggcgaca atatcgctat tgcggctacc cgcttagaca atcaagatga aaacggtaca
     1381 ggtgccgcca ttgcggcacg tgaaaacctg aatttaggca tcggacaatt aaacaaccgt
     1441 gaaaacagtc tgatttacag cggtaacgat atggcggttg gcggcgcatt agataccaat
     1501 ggccaagcca caggcaaagc ccaaaggata cacaatgccg gcgcaaccat tgaagctgca
     1561 ggcaaaatgc gtttaggtgt agaaaagctg cacaatacca atgagcattt gaaaacgcag
     1621 ttggtagaaa cagggcgcga gcatattgtt gattacgaag catttggacg acacgaatta
     1681 ttgcgagaag gcacgcaaca tgaattaggc tggtctgtct ataacgatga atcagaccac
     1741 ttacgcaccc ctgatggagc ggcgcatgaa aattggcata aatacgatta tgaaaaagtc
     1801 acccaaaaaa cccaagttac ccaaactgcg ccagccaaaa tcatttcagg taatgattta
     1861 accattgatg gtaaagaagt atttaatacc gatagccaaa tcatcgccgg cggcaatttg
     1921 cttgtgcaaa cagaaaaaga cggtttgcat aacgagcaaa cctttggcga aaagaaagta
     1981 ttcagtgaaa atggcaaatt acacagctat tggcgtgaga aacataaagg acgagactca
     2041 acgggacata gcgaacaaaa ttacactttg ccagaggaaa tcacacgcaa catttcactg
     2101 ggttcatttg cctatgaatc gcatcgcaaa gcattaagcc atcatgcgcc cagccaaggc
     2161 actgagttgc cgcaaagcaa cggtatttcg ctaccctata cgtccaattc ttttacccca
     2221 ttacccagca gcagcttata tattatcaat cctgtcaata aaggctatct tgttgaaacc
     2281 gatccacgct ttgccaacta ccgtcaatgg ttgggtagtg actatatgct ggacagcctc
     2341 aaactagacc caaacaattt acataaacgt ttgggtgatg gttattacga gcaacgttta
     2401 atcaatgaac agatcgcaga gctgacaggg catcgtcgtt tagacggtta tcaaaacgac
     2461 gaagaacaat ttaaagcctt aatggataat ggcgcgactg cggcacgctc catgaatctc
     2521 agcgttggca ttgcattaag tgccgagcaa gtagcgcaac tgaccagcga tattgtttgg
     2581 ttggtacaaa aagaagttaa acttcctgat ggcggcacac aaaccgtatt ggtgccacag
     2641 gtttatgtac gcgttaagaa tggcgacata gacggtaaag gtgcattgtt gtcaggcagc
     2701 aatacacaaa tcaatgtttc aggcagcctg aaaaactcag gcacgattgc agggcgcaat
     2761 gcgcttatta tcaataccga tacgctagac aatatcggtg ggcgtattca tgcgcaaaaa
     2821 tcagcggtta cggccacaca agacatcaat aatattggcg gcattctttc tgtcgaacag
     2881 acattattgc tcaacgcagg caacaacatc aacagccaaa gcaccgccgc cagcagtcaa
     2941 aatacacaag gcagcagcac ctacctagac cgaatggcag gtatttatat cacaggca
//
LOCUS       DAWFJW010000165         2854 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFJW010000165 DAWFJW010000000
VERSION     DAWFJW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.165"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            584..1792
                     /locus_tag="WGR19_001984"
     CDS             584..1792
                     /locus_tag="WGR19_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0799442.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1813..2124
                     /locus_tag="WGR19_001985"
     CDS             1813..2124
                     /locus_tag="WGR19_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799443.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            2128..2328
                     /locus_tag="WGR19_001986"
     CDS             2128..2328
                     /locus_tag="WGR19_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799444.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2566..2796
                     /locus_tag="WGR19_001987"
     CDS             2566..2796
                     /locus_tag="WGR19_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799445.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
BASE COUNT          865 a          484 c          682 g          823 t
ORIGIN      
        1 gatttgaggt tgcagcattt atcgggagca acagaatccg ctctgccgtc attcccacga
       61 aagtgggaat ctagtttttt gagtttcagt catttccgat aaattgcctt agcattgaat
      121 gtctagattc ccgccttata tgatgcgctc tatcaaaggg gcgcattaat tttcttaaca
      181 ttcccctttg acagccaagt gaaaggggct tttttatgtc agcagtaaat gtaatatttt
      241 cctgttccta ttggagaata tttaaaaaat cagattattg cgttttatgt ttctatcagt
      301 tcaggcatgg tgaaccgcat aaactcgctg aacaagaaaa tttttcaaag ctttatcagg
      361 cgttcgatta tatagattcg gttggctcga attttccggt aattatcaca acagacggtt
      421 gtggtctttt ttgttgatct ttaaaagttt gtcaggattt ggctttcggt cgttgaccgt
      481 cgtacgcgct ttagcgcgga agacgggaaa cggctgaaag ccccccccct tgactaacag
      541 ggggggagcg aaattaaaaa ccaattccaa gagtagtgaa cgaatgagtg aagttgaata
      601 tttctcacac tttatatcgg acggaaaagg gaagctttta gaaattccgc aacgtagggg
      661 caggcaggac ggcgttttta tagattggtt gtcattcaca ctgcacgaag attccttgct
      721 gaaagtttcc ggatgcccct tagtttccga tgccgaatat atgtttgttt taagcaaaaa
      781 attggaggaa atattagggt ttggcatcac gagcagatgc aaatcgaagg gcaataaatt
      841 ttacgattcg atgtttaggt tgggatcgga agaagttgac tacggcgaag tccattacgg
      901 aggtcagcga aatacggttt taatcgaatt gaaaggtgta ggttgcaaca ttgcaaatcc
      961 aggttgggaa ttgaggctta agcagttttt ggaagattca ttgaggccga ggataacgcg
     1021 ggtagatttg gcacttgatt tttttgatgg ggagtacacg ccggaacagg cacttttgga
     1081 tcacgataac ggttttttcg ataacagtaa catgaggccg aaatctgaaa tggttggaac
     1141 ggcttggcgg agagaggacg ggagcggcaa gacattttat gtaggtcgca agaaaaattc
     1201 tcgttttgtg cgtgtttatg agaaaggcag gcagctaggc gataaagaaa gcaagtgggt
     1261 aaggttcgaa attcagttta atcatggaga tatggaaata cctttggata ttctgataaa
     1321 tcaaggttct tacttttcag gcgctttccc gatttgtcag aaatttaaaa atatgccgaa
     1381 tccggaaagg ttcgattacc gtaaaaaagt ggctaattta acttttcagc ataaattgag
     1441 atacgcaaaa aacgcggtcg gcaaactgat taatttcatg tttgatatgg gttttgatag
     1501 tgatgaaatt gtcagatatc tgaaggcaga tttggggtat cccaaagggc tagaacctga
     1561 aaaatattcg ttggccggat tgaaggaatc tttgaaattc ggctttatcc acgaacaacc
     1621 ggatgtagat ttagaggttg aattggaaga actcggaatt atcaaattta agcaatcaga
     1681 taaattcgat ccggataaaa ggcttttcga tccacatcac gatgtagaaa gtgagaggca
     1741 atatcagctt tatctcgaca gaatgtatga tcttcatgca aatcaaaatt aacctaaaaa
     1801 ggaaaaatta atatgtttaa tcaaactcaa actgtaactt atcccgcaac ttttttagga
     1861 gctaaaaaat tcaaaggcga aattgatggc tctaatatcg acacttgttc cgtattggtt
     1921 gcaacacctt tgccggcaca gtcgggaaat gctgttggat tcacggcagc acaaatgaag
     1981 ttcggggaca gtaagaattt ctcaaaatta gagaatctca aatacccgtg cgaagttatg
     2041 gtaacggttg aaatgacttc gacaggtaag ggcatggttc cttcattaat tgattttcag
     2101 gtggcagaaa agccgaaagg ttgatttatg aaatttgaag aacgtttcat agttcaagac
     2161 ttggaaacgc atgactttat ttatcccgat cctttcggtg atgtggggtt tactcaaaat
     2221 attaaatcag caggtcaatt tgaaagctac gaagatgcgt tgaattcagg cataaatgaa
     2281 ataggcggag gattccagat atttcagttc ttcgtaaaat cggaataaaa gaaaaacagg
     2341 ctcggcgggc ggtctgtcaa cctttcacaa agcccgcaac aaaggaaaaa tatcatgaaa
     2401 atgaaccttg caacactaat tatcggctgg gtggtctgta tgtttctttt tcttttcgcc
     2461 atcttctatt ttatcggcta aaaacaggat tcgggaaaga cttcgcccgg atgaagcaag
     2521 tcaagaagtc gttttacttt aaacatcaaa aaaaggaaaa aaacgatgaa catcgttaaa
     2581 aaatatgctg taaaagcatt gttggcagcc ggtatcttca caccggctat tgttatggca
     2641 gatggctttg atgcatccgc gattggtacg caagtggcga atgtaatcat gggttttgtg
     2701 gcaatggttt ccgccgtggg tatggcggca atcaccgtaa ttcttgcaat ccaaggcttc
     2761 aaaatggctt ggagcatgat caaatctgtc aaataacggc agtgaagaaa gagggggcgc
     2821 gtaaatgggc tttcgtgtcg gtatgaattg tttt
//
LOCUS       DAWFJW010000166         2791 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFJW010000166 DAWFJW010000000
VERSION     DAWFJW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.166"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            150..473
                     /locus_tag="WGR19_001988"
     CDS             150..473
                     /locus_tag="WGR19_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0799446.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            587..2764
                     /locus_tag="WGR19_001989"
     CDS             587..2764
                     /locus_tag="WGR19_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0799447.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRRRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRPADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          669 a          992 c          709 g          421 t
ORIGIN      
        1 cccctctccc tgtgggagag ggttagggag agggcggtaa gccgcaggct tgcatcaaag
       61 tcgataacgc tttcgttaca actccgccca ctgaacgaag ccaaaacgac aaaccgcatc
      121 gtaaaccacc caacccatag gagaacccca tgcaaaacga aaccatcaac ctgaaacagc
      181 accttgccgc catcaaagaa tactggcagc ccgaaatcat caaccgccac gggttccaat
      241 tccgtttggt caaacttttg ggcgattacg gctggcatac gcacggatac agcgacaaag
      301 tgctgtttgc cgtggagggc gacatggcgg tggacttcgc cgacggcggc agcatgacga
      361 tacgcgaggg cgagatggcg gtcgtgccga agtcggtgtc gcaccgcccg cgttcggaaa
      421 acggctgctc ggtggtgctg attgagttgt ccgacccgtc cgaggccgtc tgaaaacgaa
      481 gtttccgaag gaagctgagt ttctgcgaag ctaaaagcag cctgcacctt caatcaatat
      541 gccgaaaata caaccccacc gcacaccaac acacaaagga aatcccatga cacgcttcaa
      601 atattccctg ctgtttgccg ccctgttgcc cgtgtacgcg caggccgatg tttctgtttc
      661 agacgacccc aaaccgcagg aaagcactga attgccgacc atcaccgtta ccgccgaccg
      721 caccgcgagt tccaacgacg gctacactgt ttccggcacg cacaccccgc tcgggctgcc
      781 catgaccctg cgcgaaatcc cgcagagcgt cagcgtcatc acatcgcaac aaatgcgcga
      841 ccaaaacatc aaagcgctcg accgcgccct gttgcaggcg accggcacca gccgccagat
      901 ttacggctcc gaccgcgcgg gctacaacta cctgttcgcg cgcggcagcc gcatcgccaa
      961 ctaccaaatc aacggcatcc ccgttgccga cgcgctggcc gatacgggca atgccaacac
     1021 cgccgcctat gagcgcgtag aagtcgtgcg cggcgtggcg gggctgctgg acggcacggg
     1081 cgagccttcc gccaccgtca atctggtgcg caaacgcccg acccgcaagc cattgtttga
     1141 agtccgcgcc gaagcgggca accgcaaaca tttcgggctg gacgcggacg tatcgggcag
     1201 cctgaacacc gaaggcacgc tgcgcggccg cctggtttcc accttcggac gcggcgactc
     1261 gtggcggcgg cgcgaacgca gccgcgatgc cgaactctac ggcattttgg aatacgacat
     1321 cgcaccgcaa acccgcgtcc acgcaggcat ggactaccag caggcgaaag aaaccgccga
     1381 cgcgccgctc agctacgccg tgtacgacag ccaaggttat gccaccgcct tcggcccgaa
     1441 agacaacccc gccacaaatt gggcgaacag ccgccaccgt gcgctcaacc tgtttgccgg
     1501 catcgaacac cgcttcaacc aagactggaa actcaaagcc gaatacgact acacccgcag
     1561 ccgcttccgc cagccctacg gcgtagcagg cgtgctttcc atcgaccaca acaccgccgc
     1621 caccgacctg attcccggtt attggcacgc cgacccgcgc acccacagcg ccagcgtgtc
     1681 attgatcggc aaataccgcc tgttcggccg cgaacacgat ttaatcgcgg gtatcaacgg
     1741 ttacaaatac gccagcaaca aatacggcga acgcagcatc atccccaacg ccattcccaa
     1801 cgcctacgaa ttttcccgca cgggtgccta tccgcagcca tcatcgtttg cccaaaccat
     1861 cccgcaatac ggcaccaggc ggcaaatcgg cggctatctc gccacccgtt tccgccccgc
     1921 cgacaacctt tcgctgatac tcggcggcag atacagccgt taccgcaccg gcagctacga
     1981 cagccgcaca caaggcatga cctatgtgtc cgccaaccgt ttcaccccct acacaggcat
     2041 cgtgttcgac ctgaccggca acctgtcgct ttacggctcg tacagcagcc tgttcgtccc
     2101 gcaatcgcaa aaagacgaac acggcagcta cctgaaaccc gtaaccggca acaatctgga
     2161 agccggcatc aaaggcgaat ggcttgaagg ccgtctgaac gcatccgccg ccgtgtaccg
     2221 cgcccgtaaa aacaacctcg ccaccgcagc aggacgcgac ccgagcggca acacctacta
     2281 ccgcgccgcc aaccaagcca aaacccacgg ctgggaaatc gaagtcggcg gccgcatcac
     2341 gcccgaatgg cagatacagg caggttacag ccaaagcaaa acccgcgacc aagacggcag
     2401 ccgcctgaac cccgacagcg tacccgaacg cagcttcaaa ctcttcactg cctaccactt
     2461 tgcccccgaa gcccccagcg gctggaccat cggcgcaggc gtgcgctggc agagcgaaac
     2521 ccacaccgac cctgccacgc tccgcatccc caaccccgcc gccaaagccc gcgccgccga
     2581 caacagccgc caaaaagcct acgccgtcgc cgacatcatg gcgcgttacc gcttcaatcc
     2641 gcgcgccgaa ctgtcgctga acgtggacaa cctgttcaac aaacactacc gcacccagcc
     2701 tgaccgccac agttacggcg cactgcggac agtgaacgcg gcgtttacct atcggtttaa
     2761 ataaggtcgt ctgaaaacgg agtttctgcg a
//
LOCUS       DAWFJW010000167         2779 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFJW010000167 DAWFJW010000000
VERSION     DAWFJW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.167"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(24..1133)
                     /gene="ribD"
                     /locus_tag="WGR19_001990"
     CDS             complement(24..1133)
                     /gene="ribD"
                     /locus_tag="WGR19_001990"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0799448.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWMPVSTEILGHDIKTVFRKNDNAF"
     gene            complement(1165..1629)
                     /gene="nrdR"
                     /locus_tag="WGR19_001991"
     CDS             complement(1165..1629)
                     /gene="nrdR"
                     /locus_tag="WGR19_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0799449.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            complement(1659..2501)
                     /locus_tag="WGR19_001992"
     CDS             complement(1659..2501)
                     /locus_tag="WGR19_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0799450.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDETWAQLAVDELVMFREGKIILNDRPRHPVYMSVEEGLETARAA
                     GVAV"
BASE COUNT          569 a          703 c          915 g          592 t
ORIGIN      
        1 tatagtgaaa cggcgcaaac cctttaaaag gcgttatcgt ttttccggaa cacggttttg
       61 atgtcgtgtc cgaggatttc ggtggaaacg ggcatccaca agggcggtgc ggaaagggcg
      121 gcgcggtttt cgggcaggga aaacaggtct ttgccgctgc cgaggatttt gggcgaacgg
      181 tacagcacga tttcgtccgc cagatcttct gccagaaatg cggatgtgag ttcggagcct
      241 gcttcgacca tgatttctcc gaaaccttcg tcggcaagga ggcgcatcag gtggttcagg
      301 tcgattttgc tgtctgccgt ttcagacggc atcaggatgc ggacgtgtgc gtgttcccga
      361 taggggtgca gtctgtcttc gttgcgttcg agtgtggcga tgtaggttgg agattgtccg
      421 tcggtcagaa ggtggctgtc caagggcagg cgcaggcggc tgtctaacac gatgcgtgcg
      481 ggttggcgca aagttggaaa agcgcggacg ttgagccggg gattgtccgc caacaccgtg
      541 ccgatgccgg tcagcaccgc gcagctttcg gcacgcaaaa cctgtacgtc ggcacgcgcg
      601 tcttcgccgg taatccaaaa gctgctgccg tctgaaaggg cggttttgcc gtccagcgaa
      661 acggcgcatt tgaggcggac aaaggggcgg cggcgttcga tgcgcgacag gaagcctcgg
      721 ttgagttccc ttgcctgatg ttcgagtaaa ccgcattccg tcttgatgcc tgctgcttcg
      781 agcatggcaa gccctttgcc ggcaaccagc gggttggggt cgcgcatggc ggcaacgacg
      841 cgtgttacgc cggaacggag cagagcttcg gcgcagggcg gggtacgccc gtaatggctg
      901 cacggttcga gggtaacaaa ggcggtcgcg ccttgtgcca tttcgcccgc ctgacgcagg
      961 gcgtggactt cggcatgggg ttcgcccgct ttgacgtgga agccctgccc gacaatttgg
     1021 ctgccgtgtg cgataacgca gccgacgcgc ggattgggcg aagtggaaaa acgccccaaa
     1081 gcggcaagtc ggagggcgtt ttccatcatg gatatatctg tgtccgaaaa catagggata
     1141 ccgtatcagt atgggttggg ggaatcaggc tttgccgcct gttttgacgg cttgcgccag
     1201 ccacgaggca aagtctgccg gattgtcgaa gcgtttgtgc agggcggcga aacggacggc
     1261 ggcttccgtg ttgtgctcaa gcagctcttt tagcaccatg tcggcaagtg cggcagaggg
     1321 gatgtcgcgc tgacccgaag tgtagagcct gtgttccgtc aggcggacgg tttcgtcgat
     1381 ctgttcgggt gtcagggcgg attttcgggc ggcggcggtc aggtcgttgc ggaggcgttg
     1441 tgcattaaag ggcgagcgtt ttttgtccgg accgatgacg gcgggcattt tgagttcggc
     1501 ggtttcgagc gtgccgaagc gtttgccgca gttggggcag tggcggcggc ggcgcacggc
     1561 gttgcgttct tccatcagac gcgaatcggc aacgcgggtg tcggggtggg cgcaaaatgg
     1621 gcatttcatc gggttcgtcc tcctatgtcg tctgaagttc agacggcgac gccggcggcg
     1681 cgcgcggttt ccaaaccttc ttcgacactc atataaacgg ggtgccgggg tcggtcgttc
     1741 aaaataattt tgccttcgcg gaacatcacc agttcgtcaa cggcaagttg cgcccacgtt
     1801 tcatcgcgcg tcagcggcag ggtggcgata acggcgacgc ggtcggacgg cgtggttact
     1861 tcggcaaaat cgaccatcac gtcgtcgtcg agcaggcgcg ccttgccgaa cggggcttgg
     1921 cggacgatgt agtgcagcag cgtgctggcg tgggcaaaca gggcaatgcc gtctgaaagc
     1981 ataaagttaa acagcccgaa cttgcggatt tcgtgcgtca gccccgcaat cgcgtcaaac
     2041 agcgtgtcgt cgtcgggacg ggcggcaaag cgggtgcgca ggcggttgag gatgtggcag
     2101 aacgcgcgtt cggaatcggt cgtgccgacg gggtggaaaa attcgccctg ttcggggaaa
     2161 aaatcaatca aatgtccgtt gtgggcaaac agccagtagc cgccccacat ttcgcgcata
     2221 aagggatggg tgttcgccag cgaggtttgt ccttgcgatg ctttgcggat atgggcgacg
     2281 acgttttccg atttaatctg gtaggcgcgc acgaggtcgg cgacgggcga attggcactg
     2341 ggtttgtcgt catggaacag gcgcacgcct ttgccttcga aaaagccgat gccgaaaccg
     2401 tcggcatgat ggtcggtaat gccgccgcgc cggcggaagc cttcaaagga aaacataata
     2461 tcggtcggcg tattgcagtt catgcccagc agttgacaca tagtttgtcc aaatgattca
     2521 gatggtcgca agtattcgga ttataccccg aactgaaaat gccgtctgaa atacggcttg
     2581 ttccccatta ttcccacgaa aacagaaaac aaagacggaa acttaagatt ccgtcattcc
     2641 cgcgcaggcg ggaatctgga atttcaatgc ctcaagaatt tatcggaaaa aaccaaaacc
     2701 cttccgccgt cattcccacg aaagtgggaa tctagaaatg aaaagcaacg ggaatttatc
     2761 ggaaacgacc gaaaccgaa
//
LOCUS       DAWFJW010000168         2704 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFJW010000168 DAWFJW010000000
VERSION     DAWFJW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.168"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(45..1001)
                     /locus_tag="WGR19_001993"
     CDS             complement(45..1001)
                     /locus_tag="WGR19_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0799451.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            1237..2202
                     /locus_tag="WGR19_001994"
     CDS             1237..2202
                     /locus_tag="WGR19_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0799452.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT          591 a          719 c          842 g          552 t
ORIGIN      
        1 tttaatggtt gtttcaaatc aaaatatcta tgccgccggc cccatcagca cttcttcaca
       61 tccgaaggca aaaatccgta atgccgtctg aacgcttcgt tgaaccgtcc cgcgtggcgg
      121 tagccgcaaa agtgcatggc ggcttggacg gtgctgccgg attcgatgag ggcgagcgcg
      181 tgttccagcc gcaggcggcg caggcatccg gcgacggttt cgccggtttg cgctttgaaa
      241 tagcgtttca ggtagcattc gttcagtccg acgcggcggg cgatttcggc gatggtcagc
      301 ggacgggcga attcgtgttg caggatgtcg gcggcttcgt ctatgcgccg acggcggtaa
      361 ccgttgtcgt ggcggcggaa ggtgaagcgc aataatcggg cggagagttc cagcgcggcg
      421 gcttcgtcgg caagcaggcc gaagccgtcc gattcgaacg ggcgttgcag cagtgggcag
      481 gccgccgccg tcagtgccgc tgcgttttgc gccagccgtt gcagggcgaa tcggcctatc
      541 gtttgcggcg aaaacaggcg ttcgtccagc aagccttcgt cgtgccagcg gcgcagtttt
      601 tccagcgaaa aatccaaatg cagcgcgcac atgccgctgt tgtcgggcag cagggtttcg
      661 gatacgtccg ccaactcgcc gcgtaccagc cagatttcgc cggcagacgg gcggtattcc
      721 ctgccgccca tttgtaaccg gttctgcccc gacaccatga cgaacaaggc gcagttgtgg
      781 ctgaaattgt ggatttcggt gggaaacgcg cccgttccgc cgccgcgcat ccgcgacaag
      841 gtgatgcccg agtcgaagcg gttgatgcac atttccagat gcaaaccggg ctgttttgcc
      901 tgcgcaatga gcgcgctgtc ggaacagccg tccaacgccc agccggattt atcggagcgg
      961 acataggttt ggtactggcg gtagatggcg gcggtgttca tgattggata ggaacgagtt
     1021 gtctaacaaa tgaattaaat aggaattatt accaataatc aagcgcaggg attggttgaa
     1081 acggaaaagg tcgtctgaaa gggtgtttca gacgaccttt tccgtatcgg gaatttgttt
     1141 tgccgtatcg ggaattttgc gttttgcggc gtggtttctg caggttgttt gcttaataat
     1201 aaacattctt attcgtatgc aaaggaaccg cacaccgtga aaccgcgttt ttattgggca
     1261 gcctgcgccg tcctgctgac cgcctgttcg cccgaacctg ccgccgaaaa aactgtatcc
     1321 gccgcatccg catctgccgc cacactgacc gtgccgaccg cgcggggcga tgccgttgtg
     1381 ccgaagaatc ccgaacgcgt cgccgtgtac gactgggcgg cgttggatac gctgaccgaa
     1441 ttgggtgtga atgtgggcgc aaccaccgcg ccggtgcgcg tggattattt gcagcctgca
     1501 tttgacaagg cggcaacggt ggggacgctg ttcgagcccg attacgaagc cctgcaccgc
     1561 tacaatcctc agcttgtcat taccggcggg ccgggcgcgg aagcgtatga acagttggcg
     1621 aaaaacgcga ccaccataga tctgacggtg gacaacggca atatccgcac cagcggcgaa
     1681 aagcagatgg agaccttggc gcggattttc ggcaaggaag cgcgcgcggc ggaattgaag
     1741 gcgcagattg acgcgctgtt cgcccaaacg cgcgaagccg ccaaaggcaa aggacgcggg
     1801 ctggtgctgt cggttacggg caacaaggtg tccgccttcg gcacgcagtc gcggttggca
     1861 agttggatac acggcgacat cggcctaccg cctgtagacg aatctttacg caacgagggg
     1921 cacgggcagc ctgtttcctt cgaatacatc aaagagaaaa accccgattg gattttcatc
     1981 atcgaccgta ccgccgccat cgggcaggaa gggccggcgg ctgtcgaagt attggataac
     2041 gcgctggtac gcggcacgaa cgcttggaag cgcaagcaaa tcatcgtcat gcctgccgcg
     2101 aactacattg tcgcgggcgg ctcgcggcag ttgattcagg cggcggagca gttgaaggcg
     2161 gcgtttgaaa aggcagaacc cgttgcggcg gggaaagagt agggagtcgt ctgaaaacgg
     2221 agcttccgaa ggaagcgggg ggtttctgcg aagctaaagt gcggtttcaa cgaattgaaa
     2281 agcagcctgt atgttgaaaa taccgctcaa gcaaacctac ggtttgccgc cctctcccta
     2341 gccctctccc acagggagag gggattgggt tgcaggctgc ctttaaggtt taggcaaatt
     2401 tttaacttcg ttgaagctcc gttttcagac gaccttttgg agtaccgcag gcacacgcat
     2461 cgaacggctg aatcaaagtt cagaccgatg gcagcccgca cccgagttta tgcggcaaac
     2521 agcgaggcta cggcaacccg tcccctctcc ctgtgggaga gggttaggga gagggcagta
     2581 agccgtaggc ttacatcaaa gtcgataacg ctttcacaaa gcctgtgtgc actgaaaact
     2641 gaaaggtcat ctgaaaacgg agctttcgaa ggaagcagag tttctgcgaa gctaaaaccg
     2701 aagc
//
LOCUS       DAWFJW010000169         2702 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFJW010000169 DAWFJW010000000
VERSION     DAWFJW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.169"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..329
                     /locus_tag="WGR19_001995"
     CDS             <1..329
                     /locus_tag="WGR19_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ0799453.1"
                     /translation="IATTVPAQQTFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSGFINIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLQPVA"
     gene            complement(620..1414)
                     /locus_tag="WGR19_001996"
     CDS             complement(620..1414)
                     /locus_tag="WGR19_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0799454.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1458..1562
                     /locus_tag="WGR19_001997"
                     /pseudo
     CDS             1458..1562
                     /locus_tag="WGR19_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1623..2348
                     /locus_tag="WGR19_001998"
     CDS             1623..2348
                     /locus_tag="WGR19_001998"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0799455.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLADIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          739 a          836 c          597 g          530 t
ORIGIN      
        1 tcatcgctac taccgttccg gcgcaacaaa cattcctcga tgccgccgaa ctgatgcaat
       61 ggagtataga aaccgaaggg ctgggcttga acgtcatctc gcacaagata ctcggcaaag
      121 accacgccca agtcgaattt gaagcctact tccgagacgg acaacaccga tccgcgcatc
      181 acgaactgtc cggcttcatc aacatcggcg gacaatggta ttttatcgat cctaccgttc
      241 cgcatcctgc gatgaaacaa ccctgcattt gcggatcggg caaaaaattc aaagcctgct
      301 gcggcaaata tctgcaacct gtcgcataaa aatgccgtct gaacgttcag acggcatttt
      361 taacgcgcaa aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      421 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa
      481 ggattgtacc cgtataaatg ttaagtaacg ttaattatga aaatataacc gatgtttcgg
      541 cacacaatcc gcaactgtcc ggcgcaggcg gcggcaaagc ctgtttattc aaacccttat
      601 tcctgcccat ctgtccacct tactggtttt cccgtatcca cccgacccag cgttcaaaca
      661 gcttcggttc gagcgcggca acgaccgagc gtttgaacac gtgttcacca ctccaaaacc
      721 cgtcgccttc caaagtcgtc agcctgccgc ccgcctcctc gaaaatcaac gcacccgcag
      781 catagtccca cagcttctgc ccaccgtgaa cataaacatc ataacgcccg cacgccagat
      841 aacaccaatc caacgtactg ctgcccatac tccgtatcgt tccaaaaggc gcgagcgtac
      901 tcatacggct ggaaagtttg cccgaacgca aatatttgat ttccacgccc gcaatcgcct
      961 cattgagttt tttatccacg agacgcaaag gcaggcgggt tccattaaga aacgcaccct
     1021 gcccccgttc ggcataaaaa cattcgccgc tgaccggatt gtaaatcaca cccaattcgg
     1081 cgcgcccgtt gcggacaaac gccaccgaca cggcaaaatg cggcagcccg ttgacaaaat
     1141 tgttcgtccc gtctatcgga tcgacaatcc acagcccctt ttcccccgaa tattgttccc
     1201 acaaagccga ctgttcctgc cgcgacattt cctcgcccaa catcggactg tcgattaaaa
     1261 gcggcaacgc ggcggcaaaa gccgtctgcg cggcaatgtc cgcctcgctc aacatcgaac
     1321 cgtcttcctt gcggtgagac ggcgtattca aaaaacgcgg cataatttcg gtttgcgcga
     1381 tatggcgcac gactttctgc aaacggtgta acacttccta ctgtcctcat attttgaact
     1441 tgcggcacgc gaacgtatat agtggattaa atttaaatca ggacaaggcg acgaagccgc
     1501 agacagtaca aatagtacgg caaggcgagg caacgctgta ctggtttaaa tttaatccac
     1561 tataatgtcc gtttccatca cgccgctgcg acagattata accgtccgaa ccgccaaaaa
     1621 ctatgccccg attctacctg cccgaaaacc tttccgtcgg acaaaccgtc gccctgcccg
     1681 acaacatcgt ccgccacctc aacgtcctac gcgtccgccc caacgaaaac atcaccctct
     1741 tcgacggcaa aggcaaggca cacgccgcac agctgaccgt tttggaaaaa cgccgcgccg
     1801 aagccgaaat cctgcacgaa gacacaaccg acaacgagtc cccgctcaac atcacactga
     1861 tacaatccat ctcctccggc gatcgcatgg atttcaccct gcaaaaaagc gtcgaactcg
     1921 gcgtaaccgc catacagccc gtcatcagcg aacgctgcat cgtccgcctc gacggagaac
     1981 gcgccgccaa acgcctcgca cgctggcagg aaatcgtcat ctccgcgtgc gaacaaagcg
     2041 gcaggaacac cgttcccccc gtactgccca tcatcggcta ccgtgaagca ctcgacaaaa
     2101 tgccgtctga aagcaccaag ctgattatga gcatcaaccg cgcccgcaaa cttgccgaca
     2161 tacgccaacc gtccggcgca atcgtcttta tggtcgggcc agaaggcggc tggacagaac
     2221 aggaagaaca acaggcattt gaagctggct ttcaggcggt tacactcggc aaacggattt
     2281 tacgcacaga aaccgcccca ctcgccgccc tcgccgccat gcagacgctt tggggcgatt
     2341 tcgcataaac agaaatgccg tctgaaaccc gttcagacgg cattttgcag ccgattaaga
     2401 tagtaggttc aaataagatt tcccgtgtcg tcattcccgc gaaagcggga atctagaaac
     2461 gaaaaactac agggatttat ccgaaacaac aaccctctcc gccgtcattc ccgcgcaggc
     2521 gggaatctag aaacgaaaaa ctacagggat ttatccgaaa caacaaaccc tctccgccgt
     2581 cattcccgcg caggcgggaa tctagaaatt taacgttgcg gtgatttatc ggaaatgact
     2641 gaaactcaac ggactggatt cccgcctgcg cgggaatgac aaagtggaag ttgcccgaaa
     2701 ct
//
LOCUS       DAWFJW010000170         2679 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFJW010000170 DAWFJW010000000
VERSION     DAWFJW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.170"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            567..1376
                     /locus_tag="WGR19_001999"
     CDS             567..1376
                     /locus_tag="WGR19_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0799456.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKNDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHKLYYIKKGENPAIITHQNYHRYGENDY
                     STSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            1393..>2679
                     /locus_tag="WGR19_002000"
     CDS             1393..>2679
                     /locus_tag="WGR19_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
                     /protein_id="HEZ0799457.1"
                     /translation="MNEGEVVLTPEQIQTLYGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVMMDLTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVVKREWTGIFEIVNNNIKQGNEAFKNEINSLVHDMKAAGKEFGDD
                     LNTQWNNLTQAAEIIYNDIVDNTSQGIEKGVKAIKELSEKMKNAASDLADGSAEKAKQ
                     VVEDLAQAAKEAYENAKSTAEKAAQAAREFFKGLPSFKDLAEKFRDLFPNPEGWIDDG
                     HQCFAPWVKETKKRNGKYHVYDPLALDLDGDGIETVA"
BASE COUNT          916 a          427 c          572 g          764 t
ORIGIN      
        1 aaaatatacc aaaaacagca aaacaaattg taaggatagg tataggcttt gtaaaggtaa
       61 attgtgaaaa aagcagtttt ttaaacgaat gaaacggctt cgggctgaaa tatatgctga
      121 tgccctgtcc ttcccgtata tcttgtgtgt tgtcaaagtg caggctgctt tgaaattggc
      181 attacagtcg atgaaccacc attttgcttt atttcagcgg gcttgagatg tgtataagaa
      241 tattgttttg aataaattta aagaaaatga taatcgttat tgacgatttt taaaggaaag
      301 cgtagagtgc caattctatg aagcaatacg gtaagtaaca atgaaaatat ctactgcttg
      361 ggtatagagc atatttcaca acccgtaact attcttgcgg aaacagagaa aaaagtttct
      421 cttctatctt ggataaatat atttaccctc agtttagtta agtattggaa tttataccta
      481 agtagtaaaa gttagtaaat tatttttaac taaagagtta gtatctacca taatatattc
      541 tttaactaat ttctaagctt gaaattatga gaccatatgc tactaccatt tatcaacttt
      601 ttattttgtt tattgggagt gtttttacta tgacctcatg tgaacctgtg aatgaaaaga
      661 cagatcaaaa agcagtaagt gcgcaacagg ctaaagaaca aaccagtttc aacaatcccg
      721 agccaatgac aggatttgaa catacggtta catttgattt tcagggcacc aaaatggtta
      781 tcccttatgg ctatcttgca cggtatacgc aagacaatgc cacaaaatgg ctttccgaca
      841 cgcccgggca ggatgcttac tccattaatt tgatagagat tagcgtctat tacaaaaaaa
      901 acgaccaagg ttgggttctt gagccataca accagcaaaa caaagcacac tttatccaat
      961 ttctacgcga cggtttggat agcgtggacg atattgttat ccgaaaagat gcgtgtagtt
     1021 taagcacgac tatgggagaa agattgctta cttacggggt taaaaaaatg ccatctgcct
     1081 atcctgaata tgaagcttat gaagataaaa gacatattcc tgaaaatcca tattttcata
     1141 aactttacta tattaaaaaa ggagaaaatc cggcgattat tactcatcag aactatcata
     1201 ggtatggaga gaacgattac agcactagcg taggttcctg tattaacggt ttcacggtac
     1261 ggtattaccc gtttattcgg gaaaagcagc agctcacaca gcaggagttg gtaggttatc
     1321 accaacaagt agagcaattg gtacagagtt ttgtaaacaa tccaagtaaa aaataattta
     1381 aaggatctta ttatgaatga gggtgaagtt gttttaacac cagaacaaat ccaaaccttg
     1441 tatggttatg ctttccgtgg cgatacctat ggcggttggc gttatttggc taatttgggt
     1501 gaccgttatg cggatgatgc tgccgcaatt gtcggtaagg atacaaactt aaatggtttg
     1561 aatttatgga tgaaaaaagg tgtggaaaac ctatgggatg atacggtcgg taaaaagacc
     1621 cgtttagaga aatttgatcg ggttgcactg caacatttca gccaatatgt agatctaatt
     1681 aatgaaaata atggtagatt acctaacact agtgaaattg agagaagtta ctataaagcc
     1741 gttaccgaaa atggtgtttc ttctagtgca gctattgatt tagttattaa tcgttcactt
     1801 ccggatatgg cggatggtta ttgggcatta ggtttgggga tagaagccga acgtatccac
     1861 aatgagcaag cagtaaataa tccgaacggt agcgaaaggg ataatagaaa gcagttaata
     1921 tctgctttag ataaaggatt tgatggatct tttaaagaga agcattttac ttttttacaa
     1981 tctgtgatga tggatctaac aaagttaggt gttgaatata caatagatgg ttggcaaaaa
     2041 attggaggtt ggggtaatgg gataatcaat gatttatata aaagtgttgt aaaaagagag
     2101 tggactggaa tatttgagat cgttaataat aacatcaagc aaggaaatga agcttttaaa
     2161 aatgaaatca atagcttggt tcatgatatg aaagctgctg gcaaggaatt tggagatgac
     2221 ttaaatacac agtggaataa tctcactcag gctgccgaaa taatctataa tgacatagta
     2281 gacaatacta gtcaaggaat agaaaaaggt gtcaaagcca ttaaagaatt gtctgaaaaa
     2341 atgaaaaatg ctgcttccga tttggctgac ggttcagcag agaaagctaa acaagtagtg
     2401 gaagatttgg ctcaagccgc caaagaagca tacgaaaatg ccaaatccac agccgagaag
     2461 gctgctcaag cagctcgaga attttttaag ggcttgccca gttttaaaga tctggccgaa
     2521 aaatttagag atctgttccc aaatccggaa ggctggatcg atgatggtca ccaatgtttc
     2581 gctccttggg ttaaagaaac taaaaaacgc aatggcaaat atcatgtcta cgaccccctc
     2641 gccctagatt tggacggcga cggcatagaa actgtcgct
//
LOCUS       DAWFJW010000171         2633 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFJW010000171 DAWFJW010000000
VERSION     DAWFJW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.171"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            564..1082
                     /locus_tag="WGR19_002001"
     CDS             564..1082
                     /locus_tag="WGR19_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEZ0799458.1"
                     /translation="MVAGSVSAQAAISAKPAPVTRYLSNDSAPALRQALTAESQRIRM
                     KLPEEYRQIGNLAIAKIDVKGLPQRMEAFSSFQKGEHGFISLPETKIFKPISVDKYHN
                     IASPPRGTLRNIDGEYKLLETIAQQLGNNRNVSGRIDLFTELKACQSCSNVILEFRNR
                     YPNIQLNIFTGK"
     gene            1087..1470
                     /locus_tag="WGR19_002002"
     CDS             1087..1470
                     /locus_tag="WGR19_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799459.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            1720..>2633
                     /locus_tag="WGR19_002003"
     CDS             1720..>2633
                     /locus_tag="WGR19_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799460.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQV"
BASE COUNT          898 a          571 c          512 g          652 t
ORIGIN      
        1 aagtcgcgat tgaaaataac ctattatctc aagaagagta tgctcttaga gaaaaattga
       61 tcaaaaaagc caaagggaaa ggcctattat ctttagattg gggcagcctg accgaacaag
      121 aggcaaggca gtttatctat ttgattgaga aagatcgata ttctaatcaa ttgcttgacc
      181 gatatcaaaa aaatccaagt agtttaaata atcaagaaaa aaatattctt gcatatttta
      241 ttaaccaaac ctctggaggt aacacagctt gggcagcttc gatactgaaa acgccccagt
      301 caatgggtaa tctcactatt ccttccaaag atattaataa caccttatcg aaagcctatc
      361 aaacattgag tcgttatgat tcttttgatt acaaatcagc tgttgccgca caacctgcac
      421 tttacttatt aaacggaccg cttggcttca gtgtcaaagc agctactgtg gcagcaggag
      481 gatataacat tggacaggga gcgaaagcaa tctctaatgg agaatatctg catggtacag
      541 ttcaggttgt taatggcaca ttgatggttg caggatctgt atctgcacag gctgcaatat
      601 cggccaagcc tgcacctgtt acccgttatc tgagcaatga cagtgctcct gctttaagac
      661 aagctttaac tgctgaaagc cagagaatcc gcatgaaact gccggaagag tatcgacaaa
      721 tagggaatct tgcgatagca aaaattgatg ttaaaggatt accgcaaagg atggaagcat
      781 ttagttcttt ccaaaaaggg gaacatggat ttatttcgtt acctgaaaca aaaattttta
      841 aacctatatc tgttgataaa tatcataata ttgcctctcc tcctagagga acattaagaa
      901 atatagatgg agaatataaa ttacttgaaa ctatagcaca gcaactcgga aataatcgta
      961 atgtatcagg tagaattgat ctatttacag aattaaaggc ctgtcaatct tgcagcaatg
     1021 ttattttaga gtttagaaat cgctatccaa atattcaatt aaatattttt acaggaaaat
     1081 agaatcatgg aaaaaataaa aaattatata gaattcaaga aattatttaa aaatataacg
     1141 gaaaaatttg tcagagattt agatataaca ttaattgaag cagctgaata ttctataatt
     1201 gaattcgaaa tttatttatc tgaatatccg gaagatagta catttatttt tacagcatgg
     1261 gcaatctatc tgttagaaaa taactgtctc aatgaaagag gaaaagataa ttttttcatc
     1321 gcggaaatga aaaaatgtta tcaacaaact aatttggata gaataaaatc tatctattct
     1381 tcgagtgact atcatatttt tgctaaggat gtaaacaaga ttcaacaaac atttaaatta
     1441 aatcttgcca atatcatgca gcaagagtag caactgtatt tttaaccccg tcgggcaaaa
     1501 ttaccaaaaa cggtaaatca ctaaaaatac ttttataccg ccgccgcagc aagctgtagc
     1561 ctgcatgaaa cctaaaatcc atgcgtaagg tgtgtgcttc agcacgcacg cgttccatga
     1621 tttacggctc aatgccgtct gaaaagctca caatttttca gacggcattt gttattcaag
     1681 taaatattca gattccctat atactgccca gacgcgtgcg tgctgaagac accccctacg
     1741 cttgctgcag aactttcggg taaaaccggt gtgagcatta gcgcaccgta tgccaatgag
     1801 aacagccgca tcattttgag tgccgccgat atagccgcag aaaacggcaa aatcaaactg
     1861 caatcctacg gcgaccaatc ctattacgcc ggacagggcg aactctatac cttagaacgc
     1921 cgcagctaca aaaccggcaa atggtacaac cgcaaacaca ttaccgaagt caaagaacac
     1981 aaaaacgcca agcccgacgc agtaaacctc agcgcatccc aaggtatcga catcaaatct
     2041 ggtggcagca tcgacgccta cgccaccgta ttcgatgccc ccaaaggcag cattaacatc
     2101 gaagccgggc ggaaattgac actctatgcc gtagaagagc tcaactacga caaactagac
     2161 agtcataaga aacgcaaatt tctcggcatt acttatgata aggtacatga tacctctacc
     2221 caacagatgc aaactgcctt accaagccgt gtagtggctg aatcagctaa tctgcaatca
     2281 ggttgggata ccaagttaca aggtacgcag tttgaaacca cgctgggcgg cgcggtcatc
     2341 cgtgcaggcg taggcgacca ggcacgggcc gatgccaaga ttatcctcga agggatcaaa
     2401 agcagcatcc acacagaaac cgtaagcagc agcaaatccg ccctctggca aaaacaggca
     2461 ggacgcggca gcaacatcga aaccctgcaa ctgccaagtt tcaccggccc cgttgcgccc
     2521 gtactctctg ctcccggcgg ttacattgtc gacattccga aaggcaatct gaaaacccaa
     2581 atcgaaaccc tcaccaagca gcccgagtat gcttatttga aacaacttca agt
//
LOCUS       DAWFJW010000172         2577 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFJW010000172 DAWFJW010000000
VERSION     DAWFJW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.172"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..1236
                     /locus_tag="WGR19_002004"
     CDS             <1..1236
                     /locus_tag="WGR19_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0799461.1"
                     /translation="AAIANAAFTSLATQASISLVNNKGNVGKTLKELGRSRTVKNLVV
                     AAATAGVSNKIGTSALANVSDKQWVNNMTVNLADAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKGLDQHYVAHKIAHAVAGCAAAAANKGKCQDGAIGAAVGEIV
                     GEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENN
                     AVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDA
                     KAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLA
                     QISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMD
                     GSLNQMKTGAAKGRKLNLK"
     gene            1245..1622
                     /locus_tag="WGR19_002005"
     CDS             1245..1622
                     /locus_tag="WGR19_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799462.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            1900..2274
                     /locus_tag="WGR19_002006"
     CDS             1900..2274
                     /locus_tag="WGR19_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799463.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            2345..>2577
                     /locus_tag="WGR19_002007"
     CDS             2345..>2577
                     /locus_tag="WGR19_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799464.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPYILPRCVRAEDTPYACY
                     LKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGA"
BASE COUNT          861 a          458 c          585 g          673 t
ORIGIN      
        1 gccgccatag ccaatgcagc atttacctcg ctggctaccc aagcttccat ttcgctggtt
       61 aataacaaag gcaacgtcgg caaaaccctg aaagaactgg gcagaagccg tacggtgaaa
      121 aatctggtcg tggctgctgc caccgcaggc gtatccaaca aaatcggcac gtctgcttta
      181 gccaatgtca gcgacaagca gtgggtcaac aacatgaccg tgaatctggc cgatgcgggc
      241 agtgccgcgc tgattaacac cgccgttaac ggcggtagcc tgaaagacaa tttggaagcg
      301 aatatccttg cggctttggt gaataccgcg catggggagg cggcgagtaa gatcaaagga
      361 ctggatcagc actatgtcgc ccacaaaatc gctcatgccg tagcgggctg tgcggctgca
      421 gcggcgaata agggcaaatg tcaggacggc gcgatcggtg cggctgtggg tgagattgtc
      481 ggggaggctt tggttaaaaa taccgatttt agcgatatga ccccggaaca attagatctg
      541 gaagttaaga aaattaccgc ctatgccaaa cttgcggcag gtacagttgc aggcgtaacg
      601 ggaggagatg tcaatactgc tgcacaaacc gcacaaaacg cggtagaaaa taatgcggtt
      661 aaagctgttg taactgctgc aaaagtggtt tataaggtag ccagaaaagg attaaaaaac
      721 gggaaaatca acgttagaga tttaaaacag acgttgaaag acgaaggtta taatttagcc
      781 gacaacctga ccaccttatt cgacgaaaca ttggattgga acgatgccaa agccgttatt
      841 gatattgtcg tcggaacaga gctgaatcgc gctaataaag gggaagcggc acaaaaggtc
      901 aaggaagttt tagaaaaaaa tcgtccttat atccctaata aaggtgctgt accgaatatg
      961 agtacataca tgaaaaataa tccttttgga aaacagctgg ctcaaatttc agaaaagaca
     1021 acgcttccga cgcagcaagg gcagtctgtc ttcttggtaa aaagaaacca agggttatta
     1081 aaaaccggtg ataggtttta tttagatggc caacataaaa atcatttaga ggtttttgat
     1141 aaaaatggga actttaagtt tgttctaaat atggatggtt cgcttaacca aatgaaaact
     1201 ggggcagcaa aaggtcgtaa attaaactta aaataggaaa ttttatggaa acattgaatg
     1261 atataaaaaa aatcttgatt aatgtggggc tttatcaagg gtttgatttg acagatccaa
     1321 aagtatcaga agaagttaat catgaaacag ctaatatgaa atggattaaa gattatactt
     1381 cagacgggaa ttgggataat gaatttaagg aggatttaaa aaacttttta gattatatgg
     1441 aagtatgcca attagcccta aacgataaaa atttcaaaat tgccagtaat tctttattta
     1501 tggctatgat ttacgcaggt aatctatctc ttatatttga ttcaataaaa actgatatat
     1561 caacattatt gagtgctgag tataaaaaga atagtttttc atggccatct cttgatgaat
     1621 agaaagcaag ttgtagcctg catgaaatct aaaacccatg cataaggtgt gggcttcagt
     1681 atacgcgttc catgatttac ggccatatgc cgtctgaaaa gctcaatttt ttcagacggc
     1741 atttgttatg aaagtaaata tttagattcc ctgtatactg tttagactcg tgtgtgctga
     1801 gtaagctgta gtctgcatga aacctaaaac tcgctcaaaa ttaagctaag acattagcag
     1861 ggcaagggcg aaaattgaat cttaaataag gtgattcaga tgaaaaattt taatgtagta
     1921 aaagaaagtt taagagagtt aggaattaaa caaggatttg atctttatga gaaagccaca
     1981 actgaaaaat tgaatagtga agatcctctt gacttacaat ggctttctaa ctattcatct
     2041 gattggaatg atgaattaga agaagacttt gattcttttt ttcagcatat gaaggaatat
     2101 caatatgcta ttgacaatga agacattaaa tctgcatgta gttcactatg tgaagctatg
     2161 ctctatgttg gtaatattaa aaattttttt gagtttctca aaagcgatat gattagactg
     2221 ttgagaggtg aaagtaaaac aacagacttt caatggccgc aatttgatga atagcagcaa
     2281 gctgtagcct gcatgaaacc taaaatccat gcgtaaggtg tgtgcttcag cacgcacgcg
     2341 ttccatgatt tacggctcaa tgccgtctga aaagctcaca atttttcaga cggcatttgt
     2401 tatgcaagta aatattcaga ttccctatat actgcccaga tgcgtgcgtg ctgaagacac
     2461 cccctacgct tgctatttga aacagctcca agtcaccaaa gacgtcaact ggaaccaggt
     2521 acaactggcg tacgacaaat gggactataa acaggaaggc ttaaccggag ccggagc
//
LOCUS       DAWFJW010000173         2432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFJW010000173 DAWFJW010000000
VERSION     DAWFJW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.173"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            55..342
                     /locus_tag="WGR19_002008"
     CDS             55..342
                     /locus_tag="WGR19_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799465.1"
                     /translation="MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDK
                     VIFYHNEFDEEDGYPVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            379..1353
                     /gene="mafB"
                     /locus_tag="WGR19_002009"
                     /pseudo
     CDS             379..1353
                     /gene="mafB"
                     /locus_tag="WGR19_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            1364..1825
                     /locus_tag="WGR19_002010"
     CDS             1364..1825
                     /locus_tag="WGR19_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799466.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYKGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKILKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            1851..2099
                     /locus_tag="WGR19_002011"
     CDS             1851..2099
                     /locus_tag="WGR19_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0799467.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            2126..>2432
                     /locus_tag="WGR19_002012"
     CDS             2126..>2432
                     /locus_tag="WGR19_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799468.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     "
BASE COUNT          856 a          390 c          522 g          664 t
ORIGIN      
        1 gataataggg ttgattatta tgtaggaata tatgattaca ttacgtctac cttaatgagc
       61 gatatttact atcgatccac gattgatgag catttcaaga ttatagaatt aatagaaaat
      121 aatccaaatg aaatttatga tgatggcggt ggtcaacaat tttgcctaga atttcatcat
      181 gataaggtca ttttttatca caatgaattt gatgaagaag atggttatcc agtattaagc
      241 tgttcgctgc atacttttaa aactgcttta attgcttgga atgctttttt gcaattgcct
      301 aaaagtattc attcggtggt ggagactgtg attgaggaat aagcataatt agcttaatga
      361 atagaatcag cgatatagaa aggtagaatc agatatatcc cacctaaaaa ttactctcct
      421 tcagcaccgc taccaaaagg acctaataat ggatatagtg ccgggaattt aagcaaaaaa
      481 gtcctgactt tagtttacat aaacggcaaa ctcaaattcg ggccaagcag tctgccgtct
      541 tcggcattga aagcacctgt tcccattgtc ggcaaggctg aggctccgcc cggaaaagct
      601 gtgcctgtcg gcgaagcccg cattccttca aatccaaaag cggtaaaagg aacaaccgtt
      661 accctgtcgc aggaacaacg cctagccaac ggagagctga taccgaaagg ctctgtggta
      721 acgtggtcgg acaatgcggt gaaggttgtg cggcctgatg ggaaaaagcg gagatttaat
      781 agtcgggatt gggaacagaa ggcattgccg aaaccgcaac taccgaaaga ttacagagat
      841 gacatcaaca taggtggaaa taatcctaga tttaatcagc aacggcatga aaaaatcgaa
      901 tcccacaacc caaacagaat agaggagata cgccaacgta atgccgtttc ttctgcagaa
      961 aggcaggctg atgcattaaa aaggatgcaa acgtctggcc gcacccgcct gcctgggcag
     1021 ccacaaaaag gaaaacccgg cgctgctaga gaaatgtcct cttcagacaa tccgaatcaa
     1081 acagccgagg cttttgcacg tcgatattta ggcaggaaac ctacactaat ggaacgagtc
     1141 gaaggtgaaa aattatacgg aggtaaatgt gtgggctgtt ggaaagcgca aatgccggac
     1201 ggaacctttg tggtgtatag gccagcaggt aatgcttcta tgaccaaggg aactactgcg
     1261 tctgttgata ttaataaccc aacattaaat aacttaaata ttaatttaaa aggcaagcca
     1321 acagagttaa aatttaaatt ccctgctaga taaggatgtt ttaatgaatt tagagacagc
     1381 agaaaatatt caatgcgaat ttgagtattt agcattagat ggttattttc caatgcattt
     1441 tgcatcgcat ggtcaaggaa acaaagattg gcaatttgca gttgaattca tatatagatt
     1501 attaatatgt caattagcaa ctcttgaacc tatcaacttt gaaacgaaaa atgatatttt
     1561 agacttttgc cataatttag caaaacaaag tccttttaat aatgataatg aggtgtggta
     1621 caagggggaa attgtattaa caaaaaaagg gatagattta ataaaggaat atattccaga
     1681 ggcgtttgaa gagtggaatg gaaagaaatt tgaattaaac attcctttca ttaaaatatt
     1741 aaaaaatata tttgttaatt atgaggtagc atgggatgag aataatccac tattccccat
     1801 tatatcttta aacctcggca cctgataaac atggggttta tcaagcgaca gtggaaatta
     1861 aaaagcctga tggaagttgg gaaatgaaaa aaggaagggg cggaaaacct atgacaaatc
     1921 attctatgtt cccaaaagat tgggatgagg ctagaattag ggctgaagtt acttcggctt
     1981 gggagaaaag aaaggaatta gaaggtcaaa aatggagagg tacaagtaaa tctggtgttg
     2041 aaatagaagg atttacggaa cctaatcgaa cagcttatcc actttataat agtaaatgaa
     2101 gtattgatta aaaaggtgtt tttaaatgta taaaataaaa tgtttttatg atcagtttag
     2161 ttctggtgaa ttatttaact actgccagtt tttagataat tcttctaatc aaaagataaa
     2221 tacacgagga acggtgtatc aatatattat ttatgtttta acaggagacc tttatctaca
     2281 aaaagacata gatgaaaatt tagaatttat ccatcaagca gaaaataatc ccaatgaagt
     2341 ttattcagga ggtggacaag gtttttgctg ggatatttct gctgaaaaag tagttttcta
     2401 tcacaacgaa tttgatgaag aagacggctg gc
//
LOCUS       DAWFJW010000174         2403 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFJW010000174 DAWFJW010000000
VERSION     DAWFJW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.174"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(336..782)
                     /locus_tag="WGR19_002013"
     CDS             complement(336..782)
                     /locus_tag="WGR19_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799469.1"
                     /translation="MIFEDYFSELQADMVSICLEYVQGKADKIYIYAAFEPRVSGAGC
                     FFSVKGKLVQRHLVNQEIPEVNLDHQIQVLDILMEDLEKLNSICQKFNQPMPTEIKMI
                     YDVKKNSLSANYQYNLILSSESGLLALPQDVEDAWFEEIKKQMEDN"
     gene            complement(797..>2403)
                     /locus_tag="WGR19_002014"
     CDS             complement(797..>2403)
                     /locus_tag="WGR19_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEZ0799470.1"
                     /translation="AEKAAIRASLGQVNAYLAENQSRYDTWKEGGIGRSILHGAAGGL
                     TTGSLGGILAGGGTSLAAPYLDKAAENLGTAGKAAVNALGGAAIGYATGGSGGAVVGA
                     NVDWHNRQLHPSERDWIRKNAQAFAKQEVGGREPTIDEIALAEKRLAQQAAKETDLFW
                     MLTLEKVTDLSAQKFLRQAKGTFTNENGTQQQFFTTQGKQFMRPELFANEVYDLSFYK
                     NHLQSPKNSSIGKGTGELARRVTSSTWDKAKQDPILTGLQIAASPVHLVRDVGKASWQ
                     CVSGFGQCLQQGKENFVETGSAIGTGTASFQLNDLKPIYGQDVRGAQAGLVALQTADG
                     ITTALGAAKALNVAGKGSRYFINEWASSPPSGSRAAQRGAVGNLTHSQQVLTDGSHIQ
                     RGGKLLPNIRYKAGEFDYLYETDTLGRIKSFETDNLQLTQRDRLKHNSKTPDKVKGQD
                     HAGHLIGDRFGGSPNLDNLVSQHKDVNLSAYKVLENKWAEAIKSGKNVKIQMQIEYAA
                     GTRRPTRFVGYYWIDGKPTRININNL"
BASE COUNT          643 a          546 c          411 g          803 t
ORIGIN      
        1 ttgtctaatt ttcaaaatta gctcagttgc attagattga ttatatccta gagctgattc
       61 aaaaactcta gctttatgtc tcccaacatc atgattagga ttcaatgcat aatctgtaaa
      121 ttttctggta tcgattatga cagaatcagc gttagggatt agcaagcgta gggtgggcaa
      181 ctcgttgccc acgcggtttg atacacgcca cgttatttta ttttttcagg cagcctgaaa
      241 tgaaatattt cacattaaaa catcatttta acatatacga ttgaactgcg tgggcaacga
      301 gttgcccacc ctacgcttgc tacgcttgct gtgaattaat tatcttccat ttgcttctta
      361 atttcttcaa accatgcatc ttcaacatct tgcggtaaag caagtaatcc agattcagat
      421 gacaagatta aattatattg ataatttgct gataagctat tcttttttac atcataaatc
      481 attttaattt cagttggcat aggttggtta aatttctgac aaatagaatt tagtttttct
      541 aaatcttcca taagtatgtc taatacttga atttgatggt caagattaac ttctggaatt
      601 tcttgattaa caagatgtcg ttgtacaagt tttcctttaa cagagaaaaa acatccagca
      661 ccactaacac gaggctcaaa agctgcataa atgtaaattt tatcagcctt accttgaaca
      721 tattccaaac aaatagaaac catatctgct tgtaattcac taaaataatc ttcaaaaatc
      781 ataaaaaatc cttgtttcat aaattattaa tattaattct agttggttta ccatcaatcc
      841 aataatatcc aacaaatcgt gtagggcgtc ttgtacctgc tgcatattca atttgcattt
      901 ggattttaac attttttcct gatttaattg cttccgccca tttattctcc aaaactttat
      961 acgcacttaa gtttacatct ttgtgctgtg acactaaatt atctaaattg ggcgaacctc
     1021 caaatctatc tccaattaaa tgtcctgcat ggtcttgccc cttgacttta tcaggagttt
     1081 ttgaattatg ctttaatcta tctctctgtg ttaattgcaa attatctgtt tcaaaacttt
     1141 taattctacc taaagtatca gtttcatata aataatcaaa ctcgcctgct ttatagcgaa
     1201 tattaggtaa aagttttccg ccacgttgaa tatgtgaacc atctgtcaaa acttgttgcg
     1261 aatgggttaa attccccact gccccacgct gtgcagcacg agaaccacta ggcggactac
     1321 tagcccattc atttataaaa tagcgactac cttttcctgc aacatttaat gcttttgctg
     1381 cacccaatgc cgtagttatt ccatctgccg tttgtagtgc aaccaatcct gcttgtgcac
     1441 cacgtacatc ttgaccataa atcggcttaa ggtcgtttag ttgaaaagag gcagttcctg
     1501 ttccaattgc agaacctgtt tctacaaaat tctcttttcc ttgttgcaaa cattgtccaa
     1561 agccgcttac gcactgccag cttgccttac ctacatcccg aactaaatgt acaggggagg
     1621 cagctatttg caatcctgtc aaaataggat cttgttttgc tttatcccaa gtagatgaag
     1681 taactcttcg agccaattct cctgtaccct tgccgatact actattttta ggagattgta
     1741 aatggttttt gtaaaaagac agatcatata cttcattagc aaacaattca ggacgcataa
     1801 attgcttccc ttgcgtagta aaaaattgtt gctgcgtacc attttcattt gtaaaagttc
     1861 cttttgcttg tcttaggaat ttttgagctg ataaatctgt tactttttct aatgttagca
     1921 tccaaaacaa atctgtctct ttagccgcct gttgtgctag tcgtttttct gctaatgcaa
     1981 tttcatctat agtgggttct ctaccaccta cctcctgttt cgcaaaagct tgtgcatttt
     2041 ttcttatcca atctctttca ctcggatgca actgccgatt atgccaatct acattcgcac
     2101 ccaccacagc accaccacta ccaccagttg catagccgat tgccgccccg cccagtgcgt
     2161 tgaccgccgc tttgcccgcc gtaccgaggt tttccgccgc tttgtccaaa tacggtgcgg
     2221 caagggaagt gccgccgccg gccagtatgc cgccgaggct gccggtcgtc agtccgcctg
     2281 ccgccccgtg cagtatgctc ctgcctatgc cgccttcttt ccaggtgtcg tagcggcttt
     2341 ggttttcggc aaggtaggcg tttacttggc cgagggatgc gcggatggcg gctttttcgg
     2401 ctt
//
LOCUS       DAWFJW010000175         2366 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFJW010000175 DAWFJW010000000
VERSION     DAWFJW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.175"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            1..818
                     /locus_tag="WGR19_002015"
                     /pseudo
     CDS             1..818
                     /locus_tag="WGR19_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            1253..1624
                     /locus_tag="WGR19_002016"
     CDS             1253..1624
                     /locus_tag="WGR19_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799471.1"
                     /translation="MKKIRLLLEFHCFPVWFYDENDEIIENDWCEELLPQEILSDNHL
                     KELCIWLADEFDKLYCDEMGNFDYEKLQDEFSFQTKENEQEFIDKLTEFRTKFTQKCF
                     GFCALQDNLPKIENGKIVSWQ"
     gene            2061..2270
                     /locus_tag="WGR19_002017"
     CDS             2061..2270
                     /locus_tag="WGR19_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799472.1"
                     /translation="MPVQYDYNGSKRTGYVQFLGTDSKGQSKFIFVGTNENGSITTIH
                     TKSGKDFWRTLNNNPKNKTIYPKTR"
BASE COUNT          833 a          407 c          498 g          628 t
ORIGIN      
        1 aagccgaaaa agccgccatc cgcgcatccc tcggccaagt aaacgcctac cttgccgaaa
       61 accaaagccg ctacgacacc tggaaagaag gcggcatagg caggagcata ctgcacggag
      121 cggcaggcgg actgacgacg ggcagcctcg gcggcatact ggccggcggc ggcacttccc
      181 ttgccgcacc gtatttggac aaagcggcgg aaaacctcgg tccggcgggc aaagcggcgg
      241 tcaacgtact gggcggggcg gcaatcggct atgcggcggg cgggaatgtc ggtacggcgg
      301 cagtaggggc gaatgtcgat tggaacaata ggcagctgca tccgagtgaa tataaattag
      361 cagaacaata tgcagatata gtggcttctc gtctaaacat ttcaaaagaa gaagcacttg
      421 ggcgaattat tcggcatatg caacgaaccg tagattacgc aacggctaaa gcggataatt
      481 tccgtactga tgacgccatt atttcaattt taggagcaaa caatttaccc aagcaagatc
      541 aacattacac taatccgaac tacaacaaac aatacattca aaattatgtt cgtgatttca
      601 atttagccaa taaatacaat tatttttgga aaactcctac tcaatcaagg gattataaag
      661 ttggagttgt tacagatatt ggtaaggctg cattaaatag cggtataggt atatttgaat
      721 cggctgcgaa tttagcgaca ggggagatag atccgaactc atgggcttat gtacacatgg
      781 ggcgtatagg ttatacacat cctgaatatg gaaatgctct agaaaataca atgacacttg
      841 gtgtcgctgc agttaccgct gggcgtgctg gaacacaagt aagagcgaga aatacacaac
      901 aaactaaact tgttggagag ttaacagcta atggtatcaa gtttaatccg aataatgtag
      961 ttgctattac taaaacaaca gatggaaaaa ttgtttggtt agaaacagga aatacacgag
     1021 caggactaaa ccatattata gaaaggcacg gacaggaatt tttgaaaaag ggaatatctg
     1081 aatctgaaat tcctaattat ttgatgactg cggttaaaaa tggacaaatt gtaggaatgc
     1141 aacgcacaag acctatatat gaattcacct ataacggcac aaaacaccgt gtggctattg
     1201 atataggcaa taatgggttt attgttggtg ccaaccccaa aagagtagtt aaatgaaaaa
     1261 aataagattg ttacttgaat ttcactgttt tccagtatgg ttttatgatg aaaatgatga
     1321 aattattgaa aacgattggt gtgaagaatt attaccacag gaaattttat cagacaatca
     1381 tctgaaagaa ttgtgcattt ggctagcaga tgaatttgat aaattatatt gtgatgaaat
     1441 gggtaatttc gattatgaaa agttacaaga tgaattttcg tttcaaacaa aggaaaatga
     1501 acaagagttc attgataaat taacagaatt caggacgaaa tttacacaaa aatgttttgg
     1561 tttttgtgcg ttacaagata atttaccaaa aattgaaaac ggtaaaattg tttcttggca
     1621 gtgagcgtaa cgtacgcaac ttgttgccca cgctacacaa ccacaaccct taacctcttt
     1681 tgcaggctgc tcatcaatat ctttcctatt tttgatataa acataatttt gtatcaatga
     1741 cccaatgatt aaaatgatct cacaggaagc caataaatac tgtatattca gtcttttaat
     1801 gcctatccaa gtggactcta aggttaggtt gtacacatcc tgaatatggg aatgctctag
     1861 aaaatacaat gacacttggt gtcgctgcag ttaccgctga gcgtactgga acacaagcag
     1921 gaaaaatagg aacagttaaa ccgagtagtt ttgctaatga aagtttgtta aatgaccatt
     1981 tcattaaaca cggtactgaa tttaatgtaa aaaatgcaag cgaatactta aaaattggtc
     2041 aagatactgt aaataaaggt attcctgttc aatatgatta taatggttca aaacgaactg
     2101 gatatgttca gtttttgggt actgattcca aaggtcaatc taaatttatt tttgttggaa
     2161 ccaatgaaaa tggtagtatt accacgatac atacaaaatc aggaaaagac ttttggagaa
     2221 ctttgaataa caatccaaaa aacaaaacaa tttatccaaa gacaagataa aatgttatat
     2281 aaatgtaaaa tcaaaaagtt agataacacc attgaagagg cagtattgat tgaaataaat
     2341 ggttatgaac tacattgttt tatatc
//
LOCUS       DAWFJW010000176         2356 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFJW010000176 DAWFJW010000000
VERSION     DAWFJW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.176"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(366..650)
                     /locus_tag="WGR19_002018"
     CDS             complement(366..650)
                     /locus_tag="WGR19_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799473.1"
                     /translation="MKLALKRSGKYNVYNPLALDLDGDGIETVAAKGFSGSLFDHTNN
                     GIRTATGWIAAYDGFPVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTCH"
     gene            complement(647..940)
                     /locus_tag="WGR19_002019"
     CDS             complement(647..940)
                     /locus_tag="WGR19_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ0799474.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
     gene            complement(1270..1491)
                     /locus_tag="WGR19_002020"
     CDS             complement(1270..1491)
                     /locus_tag="WGR19_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ0799475.1"
                     /translation="MTSANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWKR"
BASE COUNT          804 a          402 c          334 g          816 t
ORIGIN      
        1 ataaaaactg cttataccca actttctttt cttggaacct attattattt caactctgga
       61 tgaaaaaata gaatttaaaa atttttttct ttgatatatc gttaatatct ccctagtttt
      121 tcttaaaaaa aatataatca tatatatact acacaacatt ggtaataaat aaattaaaag
      181 cgaaaggtaa ttaaattgat aattgtaaat tatataataa acaacatacg aagaaaaaat
      241 tattatagat gatttgctat acgaataaaa aaatatccta cctagcccga accttcttat
      301 aatttgtact atgtgatcat tcatttcaat atccatttaa gctttcaatt aattttctat
      361 atttttcaat ggcatgtatc atttggttga tgatcaagcc atgtatgcaa agattggaat
      421 atggtatctg tcgtgctaat aatgcccccg ttactgttta atttgcgcac aggaaaaccg
      481 tcatatgcag caatccagcc cgtggcggtg cggatgccgt tgttggtgtg atcaaataag
      541 ctgcctgaaa agcctttggc ggcgacagtt tctatgccgt caccgtccaa atctagggcg
      601 aggggattgt aaacgttata tttgcctgaa cgtttgagag ctaatttcaa ccactctctt
      661 gcccattcag ggaattcagg cagccaatct tcaaatttcg gaaattggtc ccattctttt
      721 caagataagg gagaaaatct tgtttatacc atttttccag taactctccc cattcactta
      781 tccctcgtcc aatataatct tcgccccctt tccaagggtt tgcatcatca ggaactaagg
      841 aatatataaa cctagatact tcttctaata cattgctgta tgtatcagga tccgcaaaaa
      901 agtttgctat cttacttggt gtaaaatcat ctatattcat tatagattcg ccaagcctct
      961 cgtttccata atccttattt aattcaattc atgctgtaaa acagtagagg attttagcat
     1021 tctagaatta aggacatcat ttaaaccagt ttttaattta gttggtaaga gtttgctttg
     1081 agccatcgcc tctgtttgtt gttttatata ttgtaatcca tcaccagcag caatgaatat
     1141 atcaccaatt gcttgttgaa tatcattagg tttaaatttt cctgtttctc gtatatctcg
     1201 agatacattt cttaaattta aaaaatgtta tcgaaggatg caagtaaaga agcagtattt
     1261 gttattttgt tatctcttcc aattactgct taaaaagcta tctgcagctt gaatagcatt
     1321 attaacagta atttccattt tatttctatt gttatccaca ttatagatta aatcagataa
     1381 ggattgaata gaaccaataa atgaatccca agctttatat cccaagagtg gtgaataggg
     1441 tgttaatttc acatctccaa aaccgttaat attaaaattt gcagatgtca tatctgcttt
     1501 cctttcaagt aaattaaaaa aataacgtga ggaattaaaa ttaagaataa attaattatc
     1561 gactcaatat aatatgaatc aattagctct cttatggtaa atataagagg taaaaatatt
     1621 aaaaataaac taataaagtt cagaaaaata gtaaatggaa tattttcata tggaaaaatt
     1681 ctacaaaata caagtaatat tccatgacat attagcaaat gagtaaaatt tattataaaa
     1741 tatataaata tttccccatc gtaagatata ttcaaataaa gacatataaa caatatgaag
     1801 tatataaaat atattttttg gcatctaaaa aataaacttt tagcaatcat atataaatag
     1861 cttgtagaat aaaaaacgaa aattattaat tatcgtaggt gtgcatttat tccaacacgg
     1921 tttcatgcag cttaaaaacc aaaaattctt tctcccttta tcctttctta aaaatagcgt
     1981 tttcaaattc tgcaacgaca ggtttcattc aagtaggact gccgtgattg catcaattgc
     2041 ttcgcggcat ggcatccata tcacacaaca ggacatttat tctcgggtaa gtccaccttc
     2101 atcctcttcc gccagcagga acagttacag gctacctaaa atatgctctg tgctcggata
     2161 gtaaatattt cctttctcac tattgtttgt catttaatcg acagtctact ctttctctaa
     2221 aatttcgtca ataatagtta aatactttta tttattttaa ataatgatat acatttatgg
     2281 ttatttgttt agacagccta aaacaaacgg ggaaatcgtg ttttcccgaa catggctaat
     2341 ggatagcatt ttgttc
//
LOCUS       DAWFJW010000177         2317 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFJW010000177 DAWFJW010000000
VERSION     DAWFJW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.177"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(61..1470)
                     /gene="dacB"
                     /locus_tag="WGR19_002021"
     CDS             complement(61..1470)
                     /gene="dacB"
                     /locus_tag="WGR19_002021"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0799476.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLDAQKQLREQGILNITGHLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1635..2201
                     /locus_tag="WGR19_002022"
     CDS             1635..2201
                     /locus_tag="WGR19_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0799477.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          528 a          564 c          665 g          560 t
ORIGIN      
        1 cgagtttgtc ctagttatct aggacagccc cttaaattta atccactata ttttcctgtt
       61 tcaggctcgg cgttctttgc acatcagttt cgcatccagc cagccatcgc cgccggagat
      121 gatgttgttg gcaacgaagt tgtccaagtc gggcagcagg gaaacggcgc gaccgctgtt
      181 gatgatgacg accaccacca tcggtttgtc gcccagccag tagcctgcca atgcgcggac
      241 attgttgagt gtgcctgttt tcaggcgcag aagcccaccg ctttgtttga agcggttgcg
      301 taaagtcccg tctgtgccgg cgatgggcag cgtgtcgatg aaatcttgtg caaacgggct
      361 gaaataagcc gtttccaaca tttgcgccat cattctcgcc gttacccttt cttttctgga
      421 cagacccgaa ccgttttcca aaaccaaatc cgcaatatcg atgcccgaca cggcaagttc
      481 gcgccggacg gcagatgccg cctgttcgga aacggcgggc agtttgccgt cgccgccgag
      541 tttgaggaag acggaacgcg caattagatt gtccgaacgc ttgttcatgt ccgtcaaaat
      601 ttccttcatc ggtttggcgt gtgcaacggc aagcgtctgc gcgccttccg gcgtgtcggc
      661 tatgccgata ccgtctgaaa tccgtccgcc gccgagcagc cagtggttgg taaaactttg
      721 ccggatcagt tcgtcaagcg cgaacatccg gacaccgaca ggcttgccca aacagctctc
      781 gggaatattg ccgcgcaatt tcagcgtatt gtccgaaaaa gatgcacgca tcagtttttt
      841 gaccgaaggg caggcggctt gggaggcggt aattttcaag ttgttttggg cgaaaatatg
      901 cggcaaaggc ggatcggtga ggatgtcggt actgccggcg gcattgcgtt cggcgcgcac
      961 cataaccata ccggcagaca gcatagtcgg attggggggg gtcataaacg gcgaaccgct
     1021 gtcggcttca aaatggtcgg ggctgccgac ttcgccccac aggctgtggt cgagcatcag
     1081 gtgtcccgtg atattgagta tgccttgttc gcgcaactgt ttttgagcat caagcaggtt
     1141 ttcctgattg aaaacggggt cgccgctgcc cgcccaatat aggtttccgt caagcgtgcc
     1201 gtcgtttacc gtaccgttgc ttttaaactc ggtcgcccag cggtaattgc tgccgaaggt
     1261 tttgaaggcg gcaaacgcgg taacgagttt cattgtggag gcggggttga cggggacatc
     1321 cgagcggtgg tcaatgatga cttttccgct gtcaagctct tggacatata cggcgatttc
     1381 gttttgcgga atgcggccgg tatcgagcgc gtgtgcggcg agggaggcga gaagcagcag
     1441 cagggaggcc gctgttttgg ggaaattcat aggtaaatct gttacataaa aaaagcaatt
     1501 ataaggcaaa gccggataag tgggaacgct gtggcggcgg gccggcttgt ttgcagggga
     1561 aatcacatat ataataatcg ctatcattat gaaatgattg agacgcacaa acttaatatt
     1621 caggaggaat gattatggct aagaaaatca gtattttggt gggcagcctg cgccgtgctt
     1681 cttttgcgcg caaagtggca ttgaatgcgg cggagatgtt ccccgaaggc tggcaggcgg
     1741 aaatcgttga aatcggacat ctgccgcttt acaatttcga ttatgacgac cctgcggtgg
     1801 aagatgtgcc gctacccgaa agctacacgg ctttccgcga aacgattaag gcttcggacg
     1861 gcattttgtt cgttacgtcc gaaaataacc gcaccattcc cgcctgtttg aaaaatgcgg
     1921 tggacatcgg ctcgaaaccg aatgccgacg tggcgtggaa aaacaaaccg gcaggcatca
     1981 tcagccattc cgtcggcaag atgggcggtt acagttccca aaaaaacctg cgccttgcgc
     2041 tgtcttattt cgatatgccc gtaaccggac agccggaagt gtttttgggc aattcgccga
     2101 cgctgtttga tgaaaacggc aaattgattg actcggcaag ggattttgtt cagtcatata
     2161 ttaatcagtt tgtcggtttg attgaaagaa atgccaaata aacaaaccaa gtcgaaaggc
     2221 cgcaaaaccg gttcgggttt tgcggctttt ttatatgggc tgggcagcgt caaggtttgc
     2281 cgtttgccgg ataatgcgcg gcaaatgacc ggcgtgt
//
LOCUS       DAWFJW010000178         2317 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFJW010000178 DAWFJW010000000
VERSION     DAWFJW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.178"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(186..299)
                     /locus_tag="WGR19_002023"
                     /pseudo
     CDS             complement(186..299)
                     /locus_tag="WGR19_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            439..514
                     /locus_tag="WGR19_002024"
     tRNA            439..514
                     /locus_tag="WGR19_002024"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Glu,seq:ttc)
     gene            899..1064
                     /locus_tag="WGR19_002025"
                     /pseudo
     CDS             899..1064
                     /locus_tag="WGR19_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
     gene            complement(1114..1920)
                     /gene="thiD"
                     /locus_tag="WGR19_002026"
     CDS             complement(1114..1920)
                     /gene="thiD"
                     /locus_tag="WGR19_002026"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0799478.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            1981..2178
                     /locus_tag="WGR19_002027"
     CDS             1981..2178
                     /locus_tag="WGR19_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799479.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
BASE COUNT          551 a          590 c          598 g          578 t
ORIGIN      
        1 ttatctgaaa gtttgaggtt ctagattccc gctttcgcgg gaatgacgaa aagtggcggg
       61 aatgacgaag tttcagacgg catcgcccgc ctgttttgat atggcggcac cccccccccc
      121 gacaaaaaaa aacaatccgg aacgcatctg accgttccgg cttgttttca ggcgaatccg
      181 ccgcatcaga acatactgcg cacgcccata ttgacctgcc aagtctggcg catcgtgtgc
      241 atcgaagacc tttgcgcctc aaaataaagc tgccttccgt tgtcggcatt accacgcaaa
      301 aaaatgaatt gcttgatatt ccaatgtttt ttatatgttt ttatattgtg atgcgatcag
      361 acaaacgccc ccctgacatt tgtttagacg gcatcgtatt gctaaatttc tataagtatg
      421 tataatgtcc gtttccacgc gcccatcgtc tagaggccta ggacactgcc ctttcacggc
      481 ggcaaccggg gttcgaatcc ccgtgggcgt gccaattcaa aaacctgctt gtttcaagca
      541 ggttttttta ttatgaatcg tcattcccgc acccttcgtc attcccgcga aagcgggaat
      601 ctggagcgca ggattaaaga aaccgtttta tccgataagc ttccgtgccg acaggtctgg
      661 attcccgcct gcgcgggaag gacggcagag ggtggacgat gccgtctaaa gcctgacaag
      721 gcatttgatg ccgtctgaaa cttcgtcatt cccgcaccct tcgtcattcc cgcaaaagcg
      781 ggaatctaga gcgtagggtt gaagaaaccg ttttatccaa taagtttccg tgccgacagg
      841 tctggattcc cactttcgtg ggaatgacga gtcttttata acctgagacc tttgcaaaat
      901 acctttccct tttaaattta aagagggggg agctgaagga ttattatcgg gattgggagc
      961 tggtcgaata taaagaagaa ttgggcgcga tgcacgccaa agacgaaaac ggcaatccga
     1021 tacggtttaa atttgtaacc atgctggcga aaaagcctga ataatcctca aaaatgccgt
     1081 ctgaacattt tgtttcagac ggcattttta cgtctagtcc cgatacgccc aatgattgac
     1141 cgggccgtgt cccgcgccga tttccaaagg gcttgagatt gccgccgtga tgtaggactt
     1201 ggcagtccgt acggctttgc aaacgtctaa gccttttgcc aactcggcgg taatgcaggc
     1261 agaaaacgtg cagcccgtgc cgtgcgtgtg ggcggtcgga aagcgcgggc tgtccaattc
     1321 cagcgtttcg ttttgcgtaa acagccaatc cgtgcagcgt ccgctgctgc tgccattcaa
     1381 atgtccgcct ttgatgacga catttttgac accgtaatca agcaggattt ttgccgcacg
     1441 ttccgcatct ttacggtttt cgatatgcac gccggtcagg gcttccgcct cgggcaggtt
     1501 gggggtcaat acatccgtat cgggaagcag caggcgcgtc agtgccgcaa cggcggaatc
     1561 ctgcaacagc ggcgcaccgc ctttggcaat catcacaggg tcgagtacgc gcctgccgaa
     1621 gtcgcagtgt ttcagcttgt cggcaacgca ttcgatgatt tccgccgtgc cgagcattcc
     1681 tattttgtag gcgcggatgt caaagtcttc cctgattgct tggatttgtg cggtgatggt
     1741 ttcggtcggg acgagatgaa ccgccgacac gcccaaggta ttttgcgcgg taacggcggt
     1801 gatgacgcac gttccgaaca cgccgcgcat ctgaaacgtt ttcaaatccg cctgaatgcc
     1861 cgcaccgccg cccgaatccg aaccggcgat ggtaagcgtt tgcacaaaag agcttttcat
     1921 ttgttcctcc ttaatattgt gtccgataaa aagggctaat gcctgtaaaa aagcacggcg
     1981 atgccgtctg aaaaccgcag cgcgggggtt tgtggtgcat tatatgccgt attgcaggac
     2041 aaggcacagg gcagggcggc ggggtgcatc gtagcaactg tatttttgcc cgacggggtg
     2101 aaaaatacag ttgctacacc cactgataat gaaaaaagag agcaatgccg tctgaaccgc
     2161 gtgttgcggc gagcataaac cggcacgctt tattcgtcat ttccacaaaa acagaaacct
     2221 aaaattccgt cattcctacg aaagcgggaa tccagtgtgt tcggtttcag ttgtttttgg
     2281 gtttcgggtg atttccaaat cgaaaccttt gcaaaat
//
LOCUS       DAWFJW010000179         2265 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFJW010000179 DAWFJW010000000
VERSION     DAWFJW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.179"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..574)
                     /locus_tag="WGR19_002028"
     CDS             complement(<1..574)
                     /locus_tag="WGR19_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0799480.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPVGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWNEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="WGR19_002029"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="WGR19_002029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0799481.1"
                     /translation="MNDYTAMPSEDREIGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          547 a          582 c          609 g          527 t
ORIGIN      
        1 cgaagcgttt ggcgacttcg ttccagttga cgatttccca aaaacctttc aggtagttgg
       61 ggcggctgtt gcggtagtcg atgtagtagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggcgtagcgg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt tttcaccagc catgcccaac cggagccgaa agtaccggca gcgcaggcat
      241 tgaacgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 cgccgacagg tttgccttga cctttgggcg tgaaacccag ccagtagaaa gtgtggttcc
      361 aagtttgggc tgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagtct ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag ctcagcagcg cacatatttt acctgttttg
      661 agggatggaa accaattaaa cctgctttac gctataatag aagattgcaa tttcggcacg
      721 acagatagga tgtaccatga acgattacac agccatgccg tctgaagacc gtgagatcgg
      781 cgcattgtcg ctgcctccac attcgatgga ggcggaacaa tccgttttgg gcgggttgat
      841 gctggaaaat ccggcttggg acaggattgc cgatgtggtt tcgggagagg acttctaccg
      901 ccacgaacac cgcctgattt tccgatccat tgccaaatta atcaatgaga gccgtcccgc
      961 cgatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 atttgaatat ctgattacgc tggcgcaaaa caccccgtct gccgctaaca tccgccgcta
     1081 tgccgaaatc gtgcgcgagc gttccatcat gcgccagctc gctgaagtgg ggacggaaat
     1141 cgcacgcagc gcatacaatc cgcaaggcag ggacgcgggt cagcttttgg acgaggcgga
     1201 aaacaaagta ttccaaatcg ccgaaagcac cgccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggcgtgc cgacagggtt catcgacctc gacaaaaaaa cctcgggtct
     1381 gcaacccggc gacctgatta tcgtcgccgg tcgtccttct atgggtaaga ccgccttttc
     1441 tatcaatatc gccgaacacg ttgccgtaga aggcaagctg ccggttgccg tcttctcaat
     1501 ggagatgggc ggggcgcaac tggtcatgcg tatgctcggc tcggtcggac ggctggatca
     1561 aagcgttttg aaaaccggca ggctcgaaga cgaacactgg ggtcgcctga acgaagcagt
     1621 cgtcaaactc tccgacgcgc ccgtgtacat cgacgagacc ccgggtctga ccgcgctcga
     1681 actgcgcgcc cgtgcccgcc gtctcgcccg tcaatttaac aataagctgg gattaatcgt
     1741 catcgactac ctgcaactga tggcaggatc cggccgttcc gacaaccgag cttcggagct
     1801 gggagagatt tcacgttcgc tcaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ctgagccgca cggtcgaatc gcgtaccgac aaacgcccga tgatgtccga
     1921 ccttcgcgaa tccggtgcta tcgagcagga tgccgacctg attatgttta tgtaccgcga
     1981 cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaagat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctg agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattg
//
LOCUS       DAWFJW010000180         2256 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFJW010000180 DAWFJW010000000
VERSION     DAWFJW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.180"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..1991
                     /locus_tag="WGR19_002030"
     CDS             <1..1991
                     /locus_tag="WGR19_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0799482.1"
                     /translation="TYPDSSSSRIFLQNLKRKNDPNKPGRYSLATLNKSDIESREPTF
                     TGRQTIIRLDGRVQQIKLGQSNNEVVGFNGNSNNATFGIVSEYGVTPEADEWKKVLLP
                     WTVRGSADDNQFRTVNQKSDKYSQRYRIRDNGKHERDLGDIVNSPIVAVGGYLATSAN
                     DGMVHIFKKNGGGDDRNYSLKLSYIPGTMPRKDIQNTESTLAKELRAFAEKGYVGDRY
                     GVDGGFVLRQVERNGKDHVFMFGAMGFGGRGAYALDLTKADGNDPTKASLFDVEDNGN
                     NGNNRVELGYTVGTPQIGKTHNDKYAAFLASGYATKEITSGDNKTALYVYDLENNGNL
                     IKKIEVKDGKGGLSSPTLVDKDLDGIVDIAYAGDRGGNMYRFDLSGQDPNQWSVRTIF
                     SGNKPITSAPAISQLKDKRVVIFGTGSDLSEEDVDNNDIQSIYGIFDNDTDTGTAQDG
                     QGNGLLEQVLKKDGNTLFLSDYKRSNGSGDKGWVVKLEAGQRVTVKPTVVLRTAFVTI
                     HKYNDGGCGAETAILGINTADGGKLTKKSARPIVPADNTAVAQYSGHKKGINGKSIPI
                     GCMEKDNGIVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPDGKRAGKNN
                     RCFSQKGVRTLLMNDLDSLDITGPMCGMKRISWREVFY"
BASE COUNT          636 a          560 c          597 g          463 t
ORIGIN      
        1 ccacctaccc cgacagttcg agcagccgca tcttccttca aaatctgaaa agaaaaaacg
       61 accccaacaa acccggccgc tattccctcg caaccttgaa taagtcggat attgaaagtc
      121 gagagccgac tttcacaggg cggcaaacaa tcatccgatt ggatggcaga gtacagcaaa
      181 tcaaactggg tcaaagcaat aatgaggtcg tcggttttaa tggaaacagc aacaacgcaa
      241 ctttcggcat tgttagtgaa tatggcgtca cacctgaggc cgacgagtgg aaaaaagtgc
      301 tgctgccttg gacggttcgg ggttctgctg atgacaatca atttagaaca gttaaccaaa
      361 aatcagacaa atacagccaa agataccgca tccgcgacaa cggcaagcac gagcgcgatt
      421 tgggcgacat cgtcaacagc cccatcgtgg cggtcggcgg gtatttggct acttccgcca
      481 acgacgggat ggtgcatatc ttcaaaaaaa acggcggcgg ggatgaccgc aactatagtc
      541 tgaagctcag ctacatcccc ggcacgatgc cgcgcaagga tattcaaaac accgaatcca
      601 cccttgccaa agagctgcgc gcctttgccg aaaaaggcta tgtgggcgac cgctacggcg
      661 tggacggcgg ctttgtcttg cgccaagtcg aacggaatgg gaaagaccat gtgtttatgt
      721 tcggcgcgat gggctttggc ggcagaggcg catacgcctt ggatttgacc aaagccgacg
      781 gcaatgaccc gacaaaagcc tctttgtttg atgtagaaga taacggcaat aatggcaata
      841 atcgcgtgga attaggctac accgtcggca cgccgcaaat cggcaaaacc cacaacgaca
      901 aatacgccgc cttcctcgcc tccggttatg cgactaaaga aattaccagc ggcgacaata
      961 aaaccgcgct gtatgtgtat gatttggaaa acaacggcaa tttgattaaa aaaatcgaag
     1021 tgaaggacgg caagggcggg ctttcgtccc ccacgctggt ggataaagat ttggacggca
     1081 ttgtcgatat cgcctatgcc ggcgatcgcg gcgggaatat gtaccgcttt gatttgagcg
     1141 gtcaagatcc taatcaatgg tctgtacgca ccattttcag cggcaacaaa ccgattactt
     1201 ccgcacccgc catttcccaa ctgaaagaca aacgcgtggt tatcttcggt acgggcagtg
     1261 atttgagtga ggaagatgtt gataataatg atatacaatc tatttacggt atttttgaca
     1321 atgacacaga cacgggtacg gcgcaagacg ggcagggcaa cgggctgctc gagcaagtgc
     1381 tgaaaaaaga cggcaatacg ctgttcttaa gcgactacaa aaggtctaac ggctcgggcg
     1441 ataagggctg ggtagtgaaa ttggaagccg gacagcgcgt taccgtcaaa ccgaccgtgg
     1501 tattgcgtac cgcctttgta accatccata aatataacga cggcggctgc ggcgcggaaa
     1561 ccgccatttt gggcatcaat actgccgacg gcggcaagct gaccaagaaa agcgcgcgcc
     1621 cgattgtgcc ggccgacaat acggctgtcg cgcaatattc cggtcataag aaaggcatca
     1681 acggcaaatc catccctata ggttgtatgg aaaaagacaa tggaatcgtc tgcccgaacg
     1741 gatatgttta cgacaaaccg gttaatgtgc gttatctgga tgaaaagaaa acagacggat
     1801 tttcaacaac ggcagacggc gatgcgggcg gcagcggtat agaccccgac ggcaagcgtg
     1861 ccggcaaaaa caaccgctgc ttctcccaaa aaggggtgcg caccctgctg atgaacgatt
     1921 tggacagctt ggatattacc ggcccgatgt gcggtatgaa acgaatcagc tggcgtgaag
     1981 tcttctactg atttgcacgc gaaaatgccg tccgaaaggt tttcggacgg cattttttgc
     2041 gtttttcggg aggggcgggt tcgtaaaagg cgggctatag ggtaggcttc atctcgccaa
     2101 tctcactgaa tccatcaatt tccacaattc aattaaatac cgtcaaaccg atgccgtcat
     2161 tcccgcgcag gcgggaatct agacattcaa tgctaaggca atttatcggg aatgactgaa
     2221 actcaagaaa ctggattccc gcctgcgcgg gaatga
//
LOCUS       DAWFJW010000181         2217 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFJW010000181 DAWFJW010000000
VERSION     DAWFJW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.181"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WGR19_002031"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WGR19_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0799483.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            1949..2137
                     /locus_tag="WGR19_002032"
     CDS             1949..2137
                     /locus_tag="WGR19_002032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799484.1"
                     /translation="MPSEKSSDGIFYSDGLCGFLKLFQTAFFLPNQETAAIFPAAYRP
                     VAFRYRVRLPGSMSNNAV"
BASE COUNT          566 a          636 c          583 g          432 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg atacgccggg acacgccgac ttcggcggcg aagtagagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caataaaatc gacaagccgt ctgcacgtcc gagctgggtt atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt acgcttcagg
      601 tttgagcggc tttgccaagc tggaagaaac cgacgagagc agcgatatgc gcccgctgtt
      661 cgacaccatc ttaaaacaca cgcccgcacc gagcggcagc gcggacgagc cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggcatcg gtcgtatctt
      781 gaacggacgc atcaaacccg gccaaaccgt tgccgtcatg aaccacgagc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggcttg gaacgcgtac cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggca tcggcgtaac
      961 catcaccgac aaagacaacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagacacc gccgatgccg acgtgttccg cgtatccgga cgcggcgagc tgcacctgac
     1201 cattttgttg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccttat gaaaacctga ccgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 ccgtatgttc gtatcgccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 tcgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgagc
     1741 cagcggtacc gacgaagccg ttcgcctgac cacgccgatt aagctgacgc tggaaggtgc
     1801 ggtcgagttt atcgacgatg atgagctggt agaaatcacg ccgcaatcca tccgcctgcg
     1861 caagcgttac ctgagcgaat tggaacgccg ccgccatttc aaaaagctag attgatgttt
     1921 actggattta atgtttaaat gatactcgat gccgtctgaa aaaagttcag acggcatttt
     1981 ttattcggac gggctttgcg gcttcttgaa gctgtttcag acggcgtttt tcctacccaa
     2041 tcaagaaact gccgccattt ttccagcggc atatcgcccc gtcgcgtttc ggtatcgggt
     2101 tcggcttccc ggcagcatgt cgaacaatgc cgtttgaagg aatacgcctt ttgagtcctt
     2161 acaagcttag gaagaggata tagtggatta acaaaaatca ggacaaggcg acgaagc
//
LOCUS       DAWFJW010000182         2198 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFJW010000182 DAWFJW010000000
VERSION     DAWFJW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.182"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            354..2132
                     /locus_tag="WGR19_002033"
     CDS             354..2132
                     /locus_tag="WGR19_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0799485.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQASTTDEDDEELEPVVRTAPVLKFMIDKEGTGEKEVTEGLNWAVYFDMKGELKAG
                     AITLKAGDNLKIKQNTNENTNENTNDSSFTYSLKKDLTDLTSVETEKLSFGANGNKVN
                     ITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDDTLLNTGATTNVTNDNVTDDEKKR
                     AASVKDVLNAGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNG
                     KRTEVKIGAKTSVIAEENGKLVTGKGDEGKGLVTAKTVIDAVNKSGWRVKTTDNNQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMAPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDTNKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          687 a          541 c          551 g          419 t
ORIGIN      
        1 acacaacgcg gcaggacttt atcggaaaaa accgaaaccc caccgaccgt cattcccgca
       61 aaagcgggaa tccagcaacc gaaaaaccac aggaatctat cagcaaaaac agaaacccca
      121 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
      181 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
      241 aagttggggg aatttatcag aaaaccccca acccccaaaa accgggcgga tgccgcacca
      301 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
      361 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgtcgta tccgagctca
      421 cacgcaacca caccaaacgc gcctccgcaa ccgtggcgac cgccgtattg gcgacgctgt
      481 tgtctgcaac ggttcaggcg agtactaccg atgaagacga tgaagagtta gaacccgtag
      541 tacgcactgc tccggtgttg aaattcatga ttgataaaga aggcacggga gaaaaagaag
      601 ttacagaagg tttaaattgg gcagtatatt tcgacatgaa aggagaacta aaagccggag
      661 caatcaccct caaagccggc gacaacctga aaatcaaaca aaacaccaat gaaaacacca
      721 atgaaaacac caatgacagt agcttcacct actccctgaa aaaagacctc acagatctga
      781 ccagtgttga aactgaaaaa ttatcgtttg gcgcaaacgg caataaagtc aacatcacaa
      841 gcgacaccaa aggcttgaat tttgcgaaag aaacggctgg gacgaacggc gacaccacgg
      901 ttcatctgaa cggtattggt tcgactttga ccgatgatac gctgctgaat accggagcga
      961 ccacgaacgt aaccaacgac aacgttaccg atgacgagaa aaaacgcgcg gcaagcgtta
     1021 aagacgtatt gaatgcgggt tggaatatta agggtgttaa aactggctca acaactggtc
     1081 aatcagaaaa tgtcgatttc gtccgcactt acgacacagt cgagttcttg agcgcagata
     1141 cgaaaacaac gactgttaat gtggaaagca aagacaacgg caagagaacc gaagttaaaa
     1201 tcggtgcgaa aacctctgtt atcgctgaag aaaacggtaa gttagttact ggcaaaggcg
     1261 atgagggcaa aggcttagtt actgcaaaaa ctgtaattga tgctgtgaac aaaagcggct
     1321 ggagggtgaa aacaactgac aacaatcaaa caggtcaagc tgacaagttt gaaaccgtta
     1381 catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact gtaagtaaag
     1441 atgatcaagg caacatcact gttaagtatg atgtaaatgt cggcgatgcc ctaaacgtca
     1501 atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt tcttcgggca
     1561 aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc gtcaacatta
     1621 atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc gccacttcga
     1681 tggcgccgca gttttccagc gtttcgctcg gtgcgggggc ggatgcgccc actttgagcg
     1741 tggatgacga gggcgcgttg aatgtcggca gcaaggatac caacaaaccc gtccgcatta
     1801 ccaatgtcgc cccgggcgtt aaagaggggg atgttacaaa cgtcgcacaa cttaaaggcg
     1861 tggcgcaaaa cttgaacaac cgcatcgaca atgtggacgg caacgcgcgt gcgggcatcg
     1921 cccaagcgat tgcaaccgca ggtctagttc aggcgtatct gcccggcaag agtatgatgg
     1981 cgatcggcgg cggcacttat cgcggcgaag ccggttacgc catcggctac tccagtattt
     2041 ccgacggcgg aaattggatt atcaaaggca cggcttccgg caattcgcgc ggccatttcg
     2101 gtgcttccgc atctgtcggt tatcagtggt aagggctttt tcgcctgtct gctgttgggg
     2161 caggcggaag gtttgaaggg aagggtggcg atttgccg
//
LOCUS       DAWFJW010000183         2183 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFJW010000183 DAWFJW010000000
VERSION     DAWFJW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.183"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(664..1722)
                     /gene="alr"
                     /locus_tag="WGR19_002034"
     CDS             complement(664..1722)
                     /gene="alr"
                     /locus_tag="WGR19_002034"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0799486.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTININTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            1947..>2183
                     /locus_tag="WGR19_002035"
     CDS             1947..>2183
                     /locus_tag="WGR19_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0799487.1"
                     /translation="MKELDKIDFRILKILQKNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSG"
BASE COUNT          558 a          544 c          487 g          594 t
ORIGIN      
        1 tcctagttat ctaggacagc ccctaaattt aaaccagtac agcgttgcct cgccttgccg
       61 tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactataa
      121 aaaatccctg tgtccttatt tctgctttct tacgtcattc ttttaaaggt gggaatctgg
      181 aaaccaaaaa acaacaggaa tttatccgaa acaacaaaac ctatccgccg gcatccccgc
      241 gaaagcggga atctagtttg ttgttcaaat aaattctgaa actttaattt tgtcattccc
      301 gcgcaggcgg gaatctagaa atgaaaaacg acgagaattt atcagaaatg acggaaactc
      361 aaaaaaccgg attcccgcct gcgcgggaat gacgaaattt taggtttctg attttgattt
      421 tccgtttttg cgggaaggat ggggtttcag attgtgggca ttatcagtat tttctatttg
      481 gcaatgtctt ttataatgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
      541 cgatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatactg
      601 attttgtcaa aacgcattta tgccgtctga aaggcttcag acggcatttt tgtttggact
      661 cgattactcg atataagtga attttgcacg tttgatattg cacatcaatt cgtaagggat
      721 ggttccggcc gcttcggcaa cggtattgat gttgatcgta tcgccccaca gttcgacctc
      781 gtgtcccaaa ccttcttgcg aagcgtccag ctcgatggtc atcatatcca tagagaccct
      841 gccgatgacc cgggtcaatt tgccgtcgac ggcgacgggg gaattgcttg gggcgcggcg
      901 cggataaccg tccgcataac cgcaggcaat cagaccgacg cgcgtagatt tgctggtata
      961 aaatgttgcg ccataaccga taggggagtg cggctgtaaa acgcgttcgc cgaaaatacg
     1021 ggtggaaagc ctcatcacgg gcttcagcct gtcatcgcct cctccgaacg gggaaatgcc
     1081 gtataacgcc agaccggcgc gcccccagtc cctgcgtgct tcgggaacat tcaaaatagc
     1141 ggcggagttg gcaaggcttt cttcgccttc cagcccttcc gtacccaaat cgaatgcttc
     1201 catctgtatt tccgtcatac cgctttcggg ttcgtccgca caggaaaaat gcgaaaactt
     1261 gacaatactg tccacatatt ccgactgctt caatgccgca tatgccgaag cgtaatcatg
     1321 agggaaaaaa ccggtacggt gcattcccga atccattttc aaccagacct tgacaggttt
     1381 tttccaatgg cgggaaagca aagcctcaag ttgccattgg tttccgactg ccggccagag
     1441 cgagtattgt tcgaccgctt cgtactctga tgcttcaaat acgccttcca aaaggacaat
     1501 cggatgggta atgccgctct cccgcagcct gattccttca tcgattgtcg ccacggcaaa
     1561 accgtctgcc aagtctgcca gcgcgaaagc acatctgacc gcaccgtgtc cgtatgcgtc
     1621 ggccttcact accgccaaca gtttgcctcc gtgcatttcc tttaaaatct gataattgtg
     1681 cctaaggttg cccaacctga tctgcacgtt caaagggcgc ataaattcat tccttaccaa
     1741 acaagtgttt attagcaaaa ttaagaaatc aattagggaa ttataatcca acggcaaggc
     1801 ttttaaaata ttaaagaacc ttactctccg gaggtgaggt tgggtttgcc atagggaaag
     1861 gaggcttgtg ttttcctgat ataattgtct ttccttatca gcatccaaac cattaaaatc
     1921 aataataaac aataaggtaa agacaaatga aagagttaga taagattgat ttccgtatcc
     1981 tcaagatcct gcagaaaaat gcccgtattc ccatgacgga gcttgccgag aaagtaggat
     2041 tgtccactac cccggttacc gaacgggtac gccgtttgga gcgggaacat tatatttccg
     2101 gttatcacgc ccatctcaat ccccacctgc tgggcaaacc tttattggtt tttgtcgagc
     2161 tgaagctgca atccaaatcg ggc
//
LOCUS       DAWFJW010000184         2123 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFJW010000184 DAWFJW010000000
VERSION     DAWFJW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.184"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            124..1509
                     /locus_tag="WGR19_002036"
     CDS             124..1509
                     /locus_tag="WGR19_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0799488.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     DFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1529..2047
                     /locus_tag="WGR19_002037"
     CDS             1529..2047
                     /locus_tag="WGR19_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0799489.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          448 a          646 c          571 g          458 t
ORIGIN      
        1 gttcaaataa acaggaatat aacttcagac aaacaactta ccgcccgatt tgtgctatcg
       61 ttttcgcaca acttaaaaaa acctgacaat tttgtacttt tattacagag aaaggcttta
      121 caaatggacg gctggacaca gacgctgtcc gcgcaaaccc tgttgggcat ttcggcggcg
      181 gcaatcatcc tcattctgat tttaatcgtc aaattccgca tccacgcgct gctgacactg
      241 gtcatcgtca gcctgctgac ggctttggca accggtttgc ccacaggcag cattgtcaac
      301 gacgtactgg tcaaaaactt cggcggcacg ctcggcggcg tggcgcttct ggtcggcctg
      361 ggcgcgatgc tcggacgttt ggtcgaaaca tccggcggcg cacagtcgct ggcggacgcg
      421 ctgatccgga tgttcggcga aaaacgcgca ccgttcgcgc tgggcgttgc ctcgctgatt
      481 ttcggcttcc cgattttctt cgatgccgga ctaatcgtca tgctgcccat cgtgttcgcc
      541 accgcacggc gcatgaaaca ggacgtactg cccttcgcgc ttgcctccat cggcgcattt
      601 tccgtcatgc acgtcttcct gccgccccat ccgggcccga ttgccgcttc cgaattttac
      661 ggcgcgaaca tcggccaagt tttgattttg ggtctgccga ccgccttcat cacatggtat
      721 ttcagcggct atatgctcgg caaagtgttg gggcgcacca tccatgttcc cgttcccgaa
      781 ctgctcagcg gcggcacgca agacaacgac ctgccgaaag aacctgccaa agcaggaacg
      841 gtcgtcgcca tcatgctgat tcccatgctg ctgattttcc tgaataccgg cgtatcggcc
      901 ctcatcagcg aaaaactcgt aagtgcggac gaaacctggg ttcagacggc aaaaataatc
      961 ggttcgacac cgatcgccct tctgatttcc gtattggtcg cactgtttgt cttgggacgc
     1021 aaacgcggcg aaagcggcag cgcgttggaa aaaaccgtgg acggcgcact cgcccccgtc
     1081 tgttccgtga ttctgattac cggcgcgggc ggtatgttcg gcggcgtttt gcgcgcttcc
     1141 ggcatcggca aggcactcgc cgacagcatg gcggatttgg gcattcccgt ccttttgggc
     1201 tgtttccttg tcgccttggc actgcgtatc gcgcaaggtt cggcaaccgt cgccctgacc
     1261 accgccgccg cgctgatggc tcctgccgtt gccgccgccg actttaccga ctggcagctc
     1321 gcctgtatcg tattggcaac ggcggcaggt tcggtcggtt gcagccactt caacgactcc
     1381 ggcttctggc tggtcggccg cctcttggac atggacgtac cgaccacgct gaaaacctgg
     1441 acggtcaacc aaaccctcat cgcactcatc ggctttgcct tgtccgcact gctgttcgcc
     1501 atcgtctgac agacggaaag gatagtaaat gactacgcat tttgtcgtta tgggcgtatg
     1561 cggctgcggc aagaccaccg ccgcgctgtc cctgcagaaa cacctcggtc aatgtcccta
     1621 tgccgaaggc gacgagttcc acacccaagc caaccgcgac aagatgggcg cgggtattcc
     1681 gctgaccgat gaagaccgct atccgtggtt gggcaatctg cgcgactgga tgacgaaaca
     1741 ggcgcaaaac ggtgcgaacc acaccatcgt aacctgttcc gccctcaaac gcggctaccg
     1801 cgacattctg cgcggagccg aaggcaaagc tgccttcatc cacctcagtc cgccgcaaga
     1861 catcaacctc gagcgcatga tgtcgcgcaa aggacattac atgaaagcag ggatgctcga
     1921 ttcgcaactg gaaatcctcg aggaactggg cgaaggcgaa tacggagtca aaatcgccaa
     1981 ccccggcacg cccgaagcgg tcgaagccga tattctgaac tgggttgcct cggaaaacct
     2041 gctttgaagc aatatgccgt ctgaagcccg acacaggatg ggtttcagac ggcataaaca
     2101 tcgggaacag aatggattac att
//
LOCUS       DAWFJW010000185         2093 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFJW010000185 DAWFJW010000000
VERSION     DAWFJW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.185"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            518..1627
                     /locus_tag="WGR19_002038"
     CDS             518..1627
                     /locus_tag="WGR19_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0799490.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELL
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          629 a          489 c          448 g          527 t
ORIGIN      
        1 agttttttga gtttcagtca tttccgataa attgccttag cattgaatgt ctagattccc
       61 gccttcgcgg gaatgacggg atttgaggtt tctgtttttg attttctgtt ttcgtaggaa
      121 tgacggttta aaagttaccc gaaacctcaa aaaaaaggct gtgttttaat atagtgttga
      181 tagacgtact tggctgccat gtattcaatc gtggaaatct atatcttcgt cctcgccgaa
      241 atagtctatg cccgatatac aattttgata cacaaacttg gaaatatggg tatcgtcgcc
      301 ggagcgatag aatgcggaca gttatatata tacggttttt ttagggaagc gggcagatga
      361 agggcggatt ttgaattaac cccatgcaaa ttgacttttg cgggcggttt taccacccat
      421 atacttacaa aagccaaatt tttaaacata tatccttgat atatacacgg cgtaaacata
      481 tactggaaac atctttaaat tttccgaaat tttaaatatg agcaactgga aacccgatat
      541 tccctataac gatttaccac ccctgccgcc aaaacaggat attgaaagca aaaccatcct
      601 gaaacgttgt atagccgccc gtgcatccct tgcccgttta aagcaggcgg cagaactgat
      661 accgaatcaa gccatgctga ttaacaccct tcctgttatg gaagcccgtg caagttcgga
      721 aattgaaaac atcgtaacca caacggataa gctgtttcaa tccctgcaaa tggatacgga
      781 acggcaagac cctgccacga aagaagccct gcaataccgc accgccctgt ttgcaggcta
      841 tgaatcactg acgagccgcc ctttatgcac acaaaccgcc atcatggtct gcaacgccat
      901 caagcacccc tacgaaatgg ccatccgcaa aacaggcggc acagccctaa aaggaggcaa
      961 cagcggaaat gttgtctata ccccgcccga aggagaagaa accatacgcg gcaagctggc
     1021 aaattgggag cggtttattc acgaaagcgg cgatttagac ccgcttatca tcatggcggc
     1081 ggcacattac caatttgaag ccatccatcc gtttacggac ggcaacgggc ggacggggcg
     1141 catattgaac agcctgctat tgattgaaaa agggcttttg gatttgccta ttttgtattt
     1201 gagccgctac atcatcgaaa acagggcgga ctattaccgc ctgcttttag gcgtaaccga
     1261 acggcaggat tgggaaagct ggataatcta catcttagac ggcgtagccg acaccgccga
     1321 ttggacggta tcgaaaatag atgcgatacg ccgcctgttc gagcagacac gtcaacacat
     1381 acggacacac gcacaaggaa tctacacgca cgaactggta aatcttctgt ttgagcagcc
     1441 atatacacgc attgccaacc tagaagcggc agggatagcc aaacggcaga cggcctctaa
     1501 gtacctgaaa gagcttttag acataggtgt gctgcaagaa atcgtcatcg gcagggacaa
     1561 actattcatt catccgcgcc taatggaact attgcgggga gagggcaaca gctttacttc
     1621 attctaaccc cctcttcccc cccacatgac taacacgaaa cagggatttt gacacccgaa
     1681 ccgagacccc ttgtatttcc cccgcgaaaa gccggcatcc gcccgcgtat catgggagca
     1741 acaaaacccc tgcctaaaat tttgacttgt gcaaattggg ggtatatttg ggggtatatt
     1801 gaaaaatggc taaaataaaa tgtttaataa acaaaatgtt gaaacttaat ttcgatagag
     1861 catctgcata tcgtattgag gcgttcatgg aatttgagaa agctattttt aaataagaaa
     1921 aggtaactat taaatagcta cctttctaaa attaaatatc aacacatcgt taaaacacag
     1981 cctttatttt taacaaagtt gcaaatgttt ttttattttt ttagggaata caccaaaatc
     2041 ccgtcatttc cgcgcagtcg tgaatccgaa cgcgtccgca tggaaaccta tat
//
LOCUS       DAWFJW010000186         2053 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFJW010000186 DAWFJW010000000
VERSION     DAWFJW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.186"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(220..1413)
                     /locus_tag="WGR19_002039"
     CDS             complement(220..1413)
                     /locus_tag="WGR19_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0799491.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQAQLTEQP
                     QATNGVQGGQQGNQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVS
                     VAGGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDSWDSNNDVASQLGIFK
                     RHDDMPVSVRYDSPDFSGFSGSVQFVPAQNSKSAYTPAYYYTTATNSSTSTTFVPAVV
                     GKPGSDVYYAGLNYKNGGFAGNYAFKYARHANVGRDAFELFLLGSGSDEAKGTDPLKN
                     HQVHRLTGGYEEGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAH
                     GFDLIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVG
                     LRHKF"
BASE COUNT          524 a          515 c          477 g          537 t
ORIGIN      
        1 cagactattt ttgcaaaggt ctcactatat gtgcaaacca agccgaaaat gcgaaatacc
       61 gtctgaaaat ctttcagacg gtatttgctg tctttattgc cgtttttctt ccgtatccgg
      121 atttttgttt ggggctgaag cagattggca gtcagattgc aatcaaagaa tgaaggcaag
      181 ccgtcaaaaa caaagctatc cgctttaccg ccccgatatt tagaatttgt ggcgcaaacc
      241 gacggaggcg gcattaattt gagtgtagtt gccgatgccg gtattgcgtt tcagccaagc
      301 gccagacacg atggcggaag tgcgtttgga aaaatcataa tcaacgccgg cgatgatttg
      361 atcgtagctg gtattttcgc cttttttacc gcgttcgatc aagtcgaaac catgggcata
      421 gctgatgcgc ggaactgcat taccgaagcg gtaggaagca gtggcggcaa tttcggtcgt
      481 actgtttttg gttttgtcgg cattttcaga caaatccaac tgagccgcca aggcgagatt
      541 caagccgcct tcctcatagc cgcccgtcag gcggtgtacc tgatggtttt tcaagggatc
      601 ggtacctttg gcttcatcac tcccgctgcc gagcaagaac aactcaaaag catcacgtcc
      661 gacattggcg tgtctcgcat atttaaaggc atagttcccg gcaaaaccgc catttttgta
      721 attcagaccg gcataataca catccgatcc gggcttgccg acaacagccg gaacgaaagt
      781 agtactagta ctactgttag tcgcagtagt ataataataa gctggcgtat aggcggactt
      841 gctgttttgg gccggaacga attgaacgct gccgctgaaa ccggaaaagt ccggagagtc
      901 gtagcgtaca gaaaccggca tatcgtcgtg gcgtttgaaa atacccaatt gcgaagccac
      961 atcattattg ctgtcccaag aatcaatggc ttggctggca tcgtcaaact gatttgcaac
     1021 gcgaccggcg cgcagcgtgc cgaattcgcc tgccaagccg ataaaggatt ccctgttgcc
     1081 ccactgggtc gcgccgccgc cggcaacgga tacgtcttgc tcaagctgcc aaacagcctt
     1141 cagcccttcg cccaaatcct cactcccctt aaagccgata aacgagccga aatcactgat
     1201 tttcgtcctg atgcggcttt tggccttagt aacttttacc tgattgcctt gctgaccgcc
     1261 ttgcacaccg ttagttgctt ggggctgctc ggtcaattgc gcctggatgt tcctgccttc
     1321 tacgccggct ttgatttcgc cgtataggct gacatcggca actgccgcaa gcggcagtgc
     1381 ggacaatacg agggcggtaa gtttttttcg catatcggct tccttttgta aatttgataa
     1441 aaacctaaaa acatcgggca aacacccgat atgtcatcaa ttataccccc ccccccgcaa
     1501 aaaaccattt ttcagaacaa atatctgata aatgccgcaa cctttatttt aaaaatgatt
     1561 atattttgat ataaaacaat agcttatttt tcaaaaaacg ttgtgtttct acaacacaat
     1621 tcaagcgcag acctcgtgcg ggccgatgcg ctactgcctg aaagcagtcc cggcttttta
     1681 aaacgccata aaaaaacaca cgcggcactt tatagtggat taacaaaaac cagtacggcg
     1741 ttgcctcacc tcagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg
     1801 gttccgtgct atttgtactg tcttcggctt cgccgccttg tcctgatttt tgttaatccg
     1861 ctataaagac cgtcgggcat ctgcagccgt cattcccgcg caggcgggaa tctagacctt
     1921 agaacaacag caatattcaa agattatctg aaagtctgag attctggatt cccgcgttcg
     1981 cgggaatgac ggttcagttg ctacggttac tgtcaggttt cggttatgtt ggaatttcgg
     2041 gaaacttatg aat
//
LOCUS       DAWFJW010000187         2031 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFJW010000187 DAWFJW010000000
VERSION     DAWFJW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.187"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(194..772)
                     /locus_tag="WGR19_002040"
     CDS             complement(194..772)
                     /locus_tag="WGR19_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEZ0799492.1"
                     /translation="MSPTHEISNVERLIDEINRIHAGYHERVSKLYGGNLPNLSKTLR
                     HLDIDFVLQYRLNLHESINDYLIRSDIGDLPLAYYYRVKTTESVFDKIRWFSERESGY
                     PANKVLNDIFGVRIIADSALMEEIHAALDEWVSTKGLKNWNVQEKNGYIGTHVYFNQK
                     NNLFFPWELQIWDKADALKNIESHERDKRLFV"
     gene            complement(1085..1357)
                     /locus_tag="WGR19_002041"
     CDS             complement(1085..1357)
                     /locus_tag="WGR19_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799493.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNGMD"
     gene            complement(1545..1766)
                     /locus_tag="WGR19_002042"
     CDS             complement(1545..1766)
                     /locus_tag="WGR19_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799494.1"
                     /translation="MAVWRDYITLESHDLAEGYTYFFNENTDDCYVLKQDFINNERYE
                     KTELYSQKDKVILFPKFGEYDLVLNPDII"
BASE COUNT          648 a          424 c          309 g          650 t
ORIGIN      
        1 aaggtcgtct gacaattctc aaaacggctg aggttgtgaa tttgggcaac caatactttc
       61 tttgccagaa aaatattttc ttccaaactc aacttgtatt tgtccgtaac cacgagagtt
      121 cctttaataa ttaaatctgt acacccaagg tatgcacggt aaacggtgct atattaaaaa
      181 gacccattaa aggttaaaca aaaagtcgct tgtcccgttc atggctctca atatttttca
      241 aggcatccgc cttgtcccat atctgaagtt cccacggaaa aaacagatta tttttttgat
      301 taaaatacac atgcgttccg atatagccgt ttttttcctg aacgttccag ttttttaagc
      361 ccttcgtcga aacccattca tctaaagcag catgtatttc ttccattagt gcggaatctg
      421 cgatgattct caccccaaaa atatcattta acaccttatt cgctggatac ccggactcgc
      481 gttccgaaaa ccagcggatt ttgtcgaaaa cggactccgt ggttttaacg cggtagtagt
      541 atgccaaagg caagtccccg atgtcagatc gaatcagata gtcgttgatt gactcatgca
      601 aattaagtcg gtattgcaac acaaaatcga tgtccagatg ccggagcgtc tttgacaaat
      661 tgggaaggtt tccgccatac agtttggaaa ccctttcgtg atatccggcg tgtatgcggt
      721 taatttcgtc aatcagccgt tctacattac tgatttcatg agtgggggac attccttgtt
      781 actctttcat tccaattata tgaaaataac atactaattg ctttcaacta tatattatac
      841 aaaacaaatt tcaaaacttc cttttaattt ttcattaccg ttaatacgct aaaataggag
      901 gaattgcaaa tccaaaaaac atacacggta acagattaca ggtcatcaat gaatccaatc
      961 catcccatga agacaaagga gcgactactc ggcctccaag cagcctcccg tactgaaaac
     1021 gacattgcat taaagacagc gccgtttgat ggctaatcgt acctacgcag gcttaaagtt
     1081 tccatcaatc cataccattt gagttttcat cttcataata tggatgatca aacaaatctc
     1141 cttcccaaat acattcactc gttatacctt gggattccat gaaaggaaca taagaataaa
     1201 aatctttttc atattctcca taaaatatat tcataccttt gagccatact gcatcgcatt
     1261 catcacacag aataatttca tcatgaagat atttaggttt cgaatggtag agatagcctt
     1321 gttgacaagc aggacacatt ttcattctct tattcatgat tattccatat aatcaattta
     1381 tttttgtggg taagcaatag tgatttgatt gctgccttta gtctgtacaa caatacgtat
     1441 atagcaatct gataatttcc tggtttttat cagctttatc ctcagattcc aaacccaata
     1501 ataaaagccg tttgaaattt ttttcagacg gccttaaaac ttaattaaat aatgtccgga
     1561 tttaacacca aatcatattc accaaacttt ggaaatagaa ttaccttatc tttttgggaa
     1621 tataattctg ttttttcata tcgctcatta ttaataaaat cttgtttcaa aacatagcaa
     1681 tcatccgtat tttcattgaa gaaataagta tatccttctg ctaagtcatg agattccaaa
     1741 gttatataat ctctccatac agccatagaa agaaaataat aattcgaatt ggaatatttt
     1801 tgaaattgat gggttaaata atcccttcca aatttatcga aaaatttacc gaaaaaaaac
     1861 ctacttcctc tcgcaaggta attacatatt caggttgtgt cgccgaacct attacggcag
     1921 tatataactc ccctttaaga tgtttttcct cagcttgttt ctcagaaaaa ggcttatgaa
     1981 gtctcttata accataagac cactgctcac aataaaaaat atctttcttc a
//
LOCUS       DAWFJW010000188         2017 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFJW010000188 DAWFJW010000000
VERSION     DAWFJW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.188"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            48..248
                     /locus_tag="WGR19_002043"
     CDS             48..248
                     /locus_tag="WGR19_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799495.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            283..1725
                     /gene="aldA"
                     /locus_tag="WGR19_002044"
     CDS             283..1725
                     /gene="aldA"
                     /locus_tag="WGR19_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0799496.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVST
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
BASE COUNT          520 a          540 c          523 g          434 t
ORIGIN      
        1 ataagtgttt catttattag agaaattcag gcagcctgaa acctgttgtg gataccgttg
       61 gcagtttagc gattggttat gccacaggcg gcaatgctgg ggcttttact ggtgcgaatg
      121 cagattggaa taatcggcag ttgcattcga gtgaatataa attagcagaa caatatgcag
      181 acatagtagc ttctcgtcta aacatttcaa aagaaacttg tacggtaatt tatttgggaa
      241 taaactaaaa ctacatctga ctacaaaact ggagaacccg aaatgaaaca attggccatg
      301 tacatcaacg gacgctttga aaacgatttc aacggcgaat ggcgcgacgt attgaacccg
      361 tccaccgaag aggccatcgc ccgcgaaccc aaaggcggca gggcggacgt tgaccgcgcc
      421 gtagcggcgg cgcgtgcggc gcaaccggct tgggagcgtc tgcctgcggt cgaacgcggc
      481 gcgtatttgc gcaaaatcgc ccaaggcata cgcgaacgcg ccgacgagct gaccgacacc
      541 atcgttgccg aaggcggcaa aaccaaagac ttggcgcgcg tagaagtgat gttcaccgcc
      601 gactatctcg attaccaagc cgaatgggcg cgccgttacg aaggcgaaat catccaaagc
      661 gaccgcccgc gcgaaaacat tttattgttc aaacgtccgc tgggcgtgat tgccggcatt
      721 ttgccgtgga acttcccttt cttcctgatt gcccgcaaaa tgggacccgc tttggtaacg
      781 ggcaacacca tcgtcgtcaa acccagcagc gtaaccccga tcaactgcca catcttcgcc
      841 gaaatcgtcg atgcggtcgg attgcccgca ggcgtgttca acgtagtgaa cggtcccggc
      901 gcggaaatcg gcaatgcctt gtccgcccat ccgcaagtcg atatggtcag cctgaccggc
      961 tccgtcgaag caggccgcca agtgatggaa gccgcttccg ccaacatcac caaagtttcg
     1021 ttggaactcg gcggcaaagc ccctgccatc gtgttgaaag acgcggattt ggacttggca
     1081 gtgaaatcca tcttggcttc gcgcgtcggc aacactggtc aaatctgcaa ctgcgccgag
     1141 cgcgtctatg tccacagcag cctgaaagac gcattcattg aaaaaatgac tgccgccatg
     1201 aaaggcgtgc gctacggcaa ccctgccgaa gccgaagcag gcgcgctgga aatgggcccg
     1261 ctgattgaag agcgcgccgt caaagccgtt gccgaaaaag tggaacgggc agtcaaacaa
     1321 ggtgcgaaac tggtctgcgg cggcaaacgc gccgaagggc gcggctattt cttcgagcca
     1381 accctgctga ccgacaccga caacagtatg gacattatga aagaagaaac cttcggcccc
     1441 gtcctgcctg tttccacttt cgacacgctc gaccaagtca tcgcactggc taacgattgc
     1501 gagtttggtc tgaccagttc tgtttatacg actaatttaa acgaagcctt ctacgttacc
     1561 cgccgcctgc aattcggcga aacctacatc aaccgcgaaa actttgaagc gatgcagggt
     1621 ttccacgccg gttggaaaaa atccggcatc ggcggtgcgg acggcaaaca cggtttggaa
     1681 gaatatctgc aaacccaagt cgtttatttg gaaaccgaca tttaatgccg ctttaaaacc
     1741 ccgatagaaa atgccgtctg aacccgtttt caggttcaga cggcattttt attgcttcac
     1801 cggcaatcag tcatgaccga ggtcgatgtt tttgtctttg tatagcggat taaatttaaa
     1861 cgaagccgca gacagtacaa atagtacgga accgattcac tcggtgcttg agcaccttag
     1921 agaatcgttt ctttgagcta agtcgaggca acgccgtacc ggtttttgtt aatccgctat
     1981 attccgccat ctctaagatt tacagcgata cacgggt
//
LOCUS       DAWFJW010000189         1993 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFJW010000189 DAWFJW010000000
VERSION     DAWFJW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.189"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            23..1366
                     /locus_tag="WGR19_002045"
     CDS             23..1366
                     /locus_tag="WGR19_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0799497.1"
                     /translation="MEDKQGMTKAVAGVMTDAPTDGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            1411..1701
                     /locus_tag="WGR19_002046"
     CDS             1411..1701
                     /locus_tag="WGR19_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799498.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1706..1903
                     /locus_tag="WGR19_002047"
     CDS             1706..1903
                     /locus_tag="WGR19_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799499.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
BASE COUNT          605 a          470 c          454 g          464 t
ORIGIN      
        1 cctgtttttg aggattcgca ccatggaaga taagcaaggg atgacaaagg cggttgccgg
       61 cgtgatgacg gacgcgccga cggacggcag gaagccggca accgcttcaa accttccccc
      121 cttatctaac agggggggta cagaaaccga aacggcaggc agggttcagg aagtcttcga
      181 atgttacgaa acgtacataa cggacggtaa aggaaacctg ttaggcgttc ctcttcggcg
      241 cggtgtatca gattcggctt tcattgatca aattagcttt tcatttcatg aaaaaacctt
      301 tttcgataaa tacggcgttc gtgtaagtct tttggaagac gaagatttca ttcgcgccgc
      361 gtccatgctc gccgaagaag ttttcggttt cggtatctac aaagagtcca aaggctcggg
      421 cggtcgtttt tatgagcgct gttggttgat gggttcggaa gacgtcctat acggtcgcgt
      481 ccattttggc ggacaaaaca ataccattct tttcgaactg accggcaccg gttgcggcgt
      541 cgcaaaagaa ggctgggaat cacgactttt cgcattcctg actaatgcaa tccgcccaaa
      601 aatcacacgc gttgacatcg caaaagactt tttcaacggc gaatacagcc cgaaccaagc
      661 ccgtgaagac cgaaataaag gtatgtttac ctgtcatcac gtcaaaccaa aaggcgaatg
      721 tttggggtca gattgggaag aagacgatga agccaaaatg accaaaggca agacctatgg
      781 tatcggctcc cgtgaatcgt ccaaatatgt ccgcgtctat gaaaaaggca agcagttggg
      841 cgataaaaca agcacatgga cgcgatttga aattgaattc aaagcaaaag acatcgttat
      901 ccctttcgaa gttttgcaga atccgggcga atatttcggc ggcgcatatc cgatttgcga
      961 acgattcgtc caaaaggcaa cgcgcataca cgcggttaag gaagataagg tcatttcagc
     1021 cgaccgctac cttgaatggg taaaaaaaca gttcggacgt gcggcaaacg gtctgaaatt
     1081 catttttccc gaattggaca aagccaaact gtttgaactg attgagccga gtcatcacaa
     1141 gctgcccaag tctttggctc ccgaagccta cgactgcgcc tttttgaaag ctcaagccat
     1201 tcatgaacag cccgcattca aaccgtacaa agacccttac tatatgtacg aatattacga
     1261 gaatcttgaa aaacagcttg aacagcaaaa acacgtcaac aatgaagaaa gctataacaa
     1321 cttcatttac gacaaattcg caagactacc gatttcatgg gcttaaagtg tctgcccgaa
     1381 agacgtttaa tcacacaagg aaaccaaaaa atgaacatcc aacttcaagg ccacatcgtc
     1441 ggcgttaaaa aattcaacgg acaaatcgaa ggcaagagct tcgactattg ccgcctgatt
     1501 gtcgccacac ccttagacag ctcccaaggc aacgcattgg gcagctctac tactgaatac
     1561 gatttcggcg gctctgccaa tttcgagcag ttccgaaacg cccaatttcc gatcgaagca
     1621 aacctgaacg tagaaatcgt tactacgggc aaaacccaaa aactgaaagt catcggtttt
     1681 caactcgtga agaaaggctg attgaatgca gaaagtctat gttgtccagt ccgtatcaac
     1741 aggggacttt ctgtatctct ctcctgaaac gggcgacatc ggacatacca aattaatcac
     1801 caatgccgat tatttctacg acttcgaaga agcgattaac gcaggtttgg aagaaatcgg
     1861 caaccaatac gaatttgtcg tattcggatt tttgaaagac tgattttcgg atgttcggcg
     1921 gtcgtctgaa aaacgctcca tccattaccg ccaaacactt tttgaaggaa aatatcatga
     1981 aatttattaa cac
//
LOCUS       DAWFJW010000190         1986 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFJW010000190 DAWFJW010000000
VERSION     DAWFJW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.190"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(186..1109)
                     /locus_tag="WGR19_002048"
     CDS             complement(186..1109)
                     /locus_tag="WGR19_002048"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0799500.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          514 a          469 c          488 g          515 t
ORIGIN      
        1 ttcaatgcta aggcaattta tcgggaatga ctgaaactca aaaaactgga ttcccacttt
       61 cgtgggaatg acgggatgta ggttcgtggg aatgacgaat tttaggtttc tgattttggt
      121 tttctgtttt tgtaggaatg atgaaatttt aagttttagg aatttaccgg aaaaaataga
      181 aagcgttatc cacaagttct gatgttcagc tcgtgaaatg cgtcgggcaa atcatcgctg
      241 tcggcaaatt ccacccggtc gtaagccgtt tcgtctgcca aaaccgcgcg caagagtgcg
      301 ttgttgatgg cgtgtcccga tttgtagcct tcaaacgcgc cgacaatcgg atgtccgacg
      361 atatacaaat caccgatggc atcaaggatt ttgtggcgca caaactcatc gggatagcgc
      421 aagccttcag gattcaggac atccgtgtcg tcaatcacga tagcgttgtt caaattgccg
      481 cccaaaccca gattgtgggc gcgcatcatt tccacttcgt gcataaagcc gaaagtgcgc
      541 gcgcgcgcga tttcgtcgat gtaggatttg cctgcaaaat caatttcaaa tgtcggcgag
      601 ctgcggttga aaaccggatg gtcgaattcg atggtcagcg ttaccttaaa gccgtcatac
      661 ggcgtaaagc gcacccattt gcccgcttct ttgatttcga caggcttgag gattttcaaa
      721 aaacgctttt gcgccttttg atcgaccacg cccgcatctt gcaaaaggta aataaacggc
      781 aggctggagc cgtccataat cgggatttcg ggcgcgttca gctcaatcag cgcattgtcg
      841 atgccgtagg cggacagcgc ggacataatg tgttcgatcg tgccgacgcg cacgcctttg
      901 tcggtaacga tggtggagga aaggcgggta tcgttgatca aataaggggt cagcttgatt
      961 tgttcgccca tctcgccgtc caaatcggta cggcggaagg aaatcccgct gttttcaggc
     1021 gcggggtgca gggtcagcgc gacgcgttcg cccgaatgca gcccgactcc ggtaacgctg
     1081 atggatttcg ccaaagttct ttgcagcata aaccgcttcc ttatcaatgg ggtaagtttt
     1141 ggaataatac gataaaaccg gaaaaacaag ctatgttttt ccatagtatt tgccaatgta
     1201 tctgttttca atacgtaagc cgcataaaaa tgtatagtgg attaacaaaa atcaagacaa
     1261 ggcgacgaag ccgcccccct cctgaaaaac gcaaaaaatg ccgtccgaaa acctttcgga
     1321 cggcattttc gcgtgaaccg tcattcccac aaggacataa aaccaaaaac agcaacctaa
     1381 aattcgtcat tcccacgaaa gtgggaatct agaatcccgg actttcagat aatctttgaa
     1441 tattgctgtt attctaaggt ctggattccc gcttttgcgg gaatgacggc gacagggttg
     1501 ctgttatagt ggattaacaa aaaccagtac ggcgttgcct cgccttagct caacgagaac
     1561 gattctctaa ggtgctgaag caccgagtga atcggttccg tactatttgt actgtctgcg
     1621 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ctagccgaat tactttattt
     1681 tttgatacgt aaccggccgg ttgccgtcat tcccgcgcag gcgggaatcc agtccgcttg
     1741 ttcggtaaat gagagggcgg attgcgcgcc tgtcagataa accccgtgtt taaacgggcg
     1801 gcaatgaggt acgcgcagag ccttgaagcg caatcgatat attattttca ccaaaacgga
     1861 cgaccccgcc tgccttgcaa acctttaaaa ggaagccacc cggattaatc cgagtggccg
     1921 tggaaaatca cttaccgctt gatttattta aaatttatgg tataatttac cttagctggc
     1981 accact
//
LOCUS       DAWFJW010000191         1929 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFJW010000191 DAWFJW010000000
VERSION     DAWFJW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.191"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..579
                     /locus_tag="WGR19_002049"
     CDS             <1..579
                     /locus_tag="WGR19_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEZ0799501.1"
                     /translation="KASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILN
                     ALDGKPLPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKT
                     ICALLEELAPEKPAGVARYEDLITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESG
                     LRKTVQWYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            642..1508
                     /gene="rfbA"
                     /locus_tag="WGR19_002050"
     CDS             642..1508
                     /gene="rfbA"
                     /locus_tag="WGR19_002050"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0799502.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            1553..>1929
                     /gene="rfbC"
                     /locus_tag="WGR19_002051"
     CDS             1553..>1929
                     /gene="rfbC"
                     /locus_tag="WGR19_002051"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0799503.1"
                     /translation="MNIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADCSF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFCVLGDAA"
BASE COUNT          499 a          534 c          485 g          411 t
ORIGIN      
        1 aaagcgtcca gcgaccacct cgtccgcgcg tggttgcgta cttacggctt gccgaccatt
       61 gtaaccaact gctccaacaa ctacggtcct taccattttc cggaaaaact cattcctttg
      121 atgattctga acgcgcttga cggcaaaccg ctgcctgtgt acggcgacgg tatgcaaatc
      181 cgcgactggc tgtttgtcga agaccacgcg cgcgcactgt atcaggttgt taccgaaggt
      241 gttgtcggcg aaacctacaa tatcggcggc cacaatgaaa aagccaatat tgaagtcgtc
      301 aaaaccatct gcgccctgct ggaagaactc gctcccgaaa aaccggccgg tgtggcgcgt
      361 tatgaagatt tgattacttt cgtacaagac cgccccggcc atgacgtacg ctacgccgtc
      421 gacgcagcca aaatcaggcg ggatttgggc tggctgcctt tggaaacctt cgagtccggc
      481 ctccgcaaaa ccgtgcaatg gtatctggac aacaaaacct ggtggcaaaa tgtattgaac
      541 ggcagctatc gtttggaacg tttaggtact ggaaaatagt tttcagacgg catcccgacg
      601 caatgccgtc tgaaaaccca tcgcaaagga agaaagaaaa gatgaaaggc atcatactgg
      661 caggcggcag cggcacgcgc ctctacccca tcacgcgcgg cgtatccaaa cagctcctgc
      721 ccgtgtacga taaaccgatg atttattacc ccttgtcggt tttgatgctg gcgggaatcc
      781 gcgatatttt ggtgattacc gcgcctgaag acaacgcctc tttcaaacgc ctgcttggcg
      841 acggcagcga tttcggcatt tccatcagtt atgccgtgca acccagtccg gacggcttgg
      901 cacaggcatt tatcatcggc gaagaattta tcggcaacga caatgtttgc ttggttttgg
      961 gcgacaatat tttttacggt cagtcgttta cgcaaacatt gaaacaggcg gcagcgcaaa
     1021 cgcacggcgc aaccgtgttt gcttatcagg tcaaaaaccc cgaacgtttc ggcgtggttg
     1081 aatttaacga aaacttccgc gccgtttcca tcgaagaaaa accgcaacgg cccaaatccg
     1141 attgggcggt aaccggcttg tacttctacg acaaccgtgc cgtcgagttc gccaaacaaa
     1201 tcaaaccgtc cgcacgcggc gaattggaaa ttaccgacct caaccggatg tatttggaag
     1261 acggctcgct ctccgttcaa atattgggac gcggtttcgc gtggctggac accggtacgc
     1321 acgaaagcct gcacgaggcg gcttcgttcg tccaaaccgt gcaaaacatc caaaacctgc
     1381 acatcgcctg cctggaagaa atcgcttggc gcaacggctg gctgacgaaa aaagatgtgg
     1441 aaacacgggc aaagcctttg gaaaaaaccg cctacgggca atacctgctg cgcctaatcg
     1501 gcaaataaat gccgtctgaa ccactttctt cagacggcat acagagaaaa tcatgaacat
     1561 catcgacacc gccctccccg acgtaaaact tttaaaaccg caagtcttca cagacgggcg
     1621 cggctttttt atggaaacct tccgcgacgg gtggttcaaa gaaaatattg ccgactgttc
     1681 ctttgttcag gaaaaccact ccaaatccgg caaaggcgta ttgcgcggcc tgcactacca
     1741 aaccgaaaac acacaaggca aactcgtacg cgtggttgtc ggcgaagtat tcgacgtggc
     1801 cgtcgatatg cgtaaagact cccccacttt cggcaaatgg gtaggcgaga ttctgtccgc
     1861 agaaaacaaa cgccaactgt gggtacccga aggcttcgcg cacgggtttt gcgttttggg
     1921 cgacgcagc
//
LOCUS       DAWFJW010000192         1845 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFJW010000192 DAWFJW010000000
VERSION     DAWFJW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.192"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..662)
                     /gene="neuA"
                     /locus_tag="WGR19_002052"
     CDS             complement(<1..662)
                     /gene="neuA"
                     /locus_tag="WGR19_002052"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0799504.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AE"
     gene            complement(666..1799)
                     /gene="neuC"
                     /locus_tag="WGR19_002053"
     CDS             complement(666..1799)
                     /gene="neuC"
                     /locus_tag="WGR19_002053"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0799505.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKCQSEKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
BASE COUNT          540 a          326 c          387 g          592 t
ORIGIN      
        1 ttttctgcct gttgtaaatc aagctcagta tcaatatcga tagagtcttg atgagacata
       61 atataaagtt tggttggggc gataaaaaaa caattatttg caattagtga agcagtatca
      121 ttaatgtaaa ttgcaccatt aggcctaaat gcctgaggta attgttggcg aggctgctcc
      181 aaatcgctta gatggcgcat gggggcatat tcgccattat tgatttgaag cagggttttt
      241 agtggatgat gctccattgg gcatgcagag acaacggatc cttttatttt ctcatcaaat
      301 agagaaaaag cttcacgaat atgagcccct gtgcgtaatg gactggttgg ttgtaatagg
      361 gttactgtgc cggaattact gccaattgtt tctaaagcat gtattacacc tgaaatagag
      421 ctggctgtat cggaggccag ctctgcaggg cgtaggacga cttcgacacc gaaattttta
      481 gcttcttctg caattaaccc gccatcagtc gaaacaatta tgcggtcaaa acactttgat
      541 gatatagcag cattaattgt atgaccaagt aatgatatgc cattcatttt ccggagattt
      601 tttaatggca atcctttgga gttttggcgc gcaagtataa ccgcaatatt ttgtttttcc
      661 ataatttaaa gattcaaatc gataaaacgt ttttgagcag aaacattcca cgtttcagga
      721 ttgttgatta cttcagcaaa tctttctgtg ctggtgcgag tatctccgcc attaaaggta
      781 tcatctgctt caaatttgcc taaactgcat gcttgttgaa tcgcatcaaa gatattttta
      841 gtttcataat ctgtatgaat aatagatttt cccatatggc ggttactttg gcgtgtacca
      901 acatcaattg aagggacacc gtagagagga gcttctctaa tacctgcact tgagttgccg
      961 accataaatt tagcatgttt caataagact aaaaagtatt caaatcgtat agatggaaat
     1021 gcgataaatt tttcagattg acattttaat aactcttgta aaatactttc agtacccgtg
     1081 tcgttgttag ggtaaatgct aatgatattt tggccactta attctaatgc tttgaaatat
     1141 tgggccgcat attgtggcat taaatgtgct tctgtagtca cggggtgaaa catagaaata
     1201 ccataatttt cgtatggcaa accgtaatat tctttgactt cttctaagga tgggagggtg
     1261 gaagaggcca taacatctaa atcgggggag ccgatgatgt gaatatgctt tcttttttct
     1321 cccatttgca ctaggcgagt gacagcttgt tcatttgcta ccaagtggat atgagaaagt
     1381 ttactaatag aatgacgaat ggagtcatct actgtaccag atagttcacc accttcgata
     1441 tggcaaacta aacggctgct taatgcacct acagctgcgc ctgctagtgc ttctaaacgg
     1501 tcgccgtgaa tcatgaccat atcaggttca atttcatcag atagacgaga gataaacgta
     1561 atggtattgc ctaaaacggc acccattggt tcaccttgga tttgatttga aaacagatat
     1621 gtatgttgat agttttctcg agttacttcc ttgtaggttc tgccatatgt tttcatcata
     1681 tgcataccag ttacaatcaa atgcaattca aggtctgggt gattttcaat ataggctaat
     1741 aaaggtttta gcttgccgaa gtcggctctg gtacctgtaa tgcaaagaat tcttttcatg
     1801 attttagaat ctataagtat ataagtataa ggaagttgga aagaa
//
LOCUS       DAWFJW010000193         1830 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFJW010000193 DAWFJW010000000
VERSION     DAWFJW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.193"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            105..1562
                     /locus_tag="WGR19_002054"
     CDS             105..1562
                     /locus_tag="WGR19_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0799506.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          393 a          548 c          430 g          459 t
ORIGIN      
        1 agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa aacaccgctg ccgtccgcat
       61 ccgtttcaga cggtattgag agaaaatctt ttaggagaac ctttatgtcc cggcatcccg
      121 cccccaccgg agaaaaaaca ttcttcggcc accccttcca gctttccacc ctcttccata
      181 tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg catcctgctg atttacctct
      241 actacaccgc cgacaaaggc ggcttgggca tagacaaaac cctcgccggc ggcattgtcg
      301 gcgcatacag cggcagcgtg tacctgtcca ccattttggg ggcgtggttt gccgaccgag
      361 tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt cgtgatgctc ggacacatcg
      421 tccttgccgc cgccccgggc ctgtacggcc ttttaatcgg gctgatattc atcgcattgg
      481 gcagcggcgg cgtgaaatct acggccagtt ctatggtggg cgcattatac gaacaggacg
      541 aaatgcgccc gctgcgcgat gcgggatttt ccattttcta catcgccatc aacatcggcg
      601 gcttcctggg cccgctgctg accggcctgc tgcaggaaaa catcggtttc cattatggtt
      661 tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg ttattccctg ggacgtaaaa
      721 acctgcccca ccccaccgtc ccccatccgc tttcaaaagg acagggcaaa actgcggccg
      781 ccgtcggcat cgccctcatc gccgcacttg caaccgccat caaaacaggg cttgtcaacc
      841 tcgacaattt ctccggcatc ctcctatcta ccgtcatcct tgccgtcatc gtctatttta
      901 cccggctgct gaccaacccc cgcgtcagtt ccgacaacaa gcggcatatc atcgcctaca
      961 tcccgctttt cctgaccatc tgtatgtttt gggcggtctg gtttcagatt tacaccgtgg
     1021 caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg ttcgtttacc gtacccgtcg
     1081 cttggaaaga ttctatgcaa agcctgtggg tcatcctgtt ttccggactg atggcggcaa
     1141 tgtggacaaa aatggggcgc aaacagccca aaaccccgct gaaattcgct atggcggtat
     1201 ttgttaccgg cgcgtcgttt ttgggattcg tcccctttat ttccgccggc acgccgatgc
     1261 ctattgcggt tttcgcactg attgtccttg ccatcacgat aggcgaactg atgatttccc
     1321 cgattgcgct gtccatctcc accaaaatcg caccgccttt attcaaaacc caaatggtcg
     1381 cccttaattt ccttgccttt tcattaggct tcactttggg cggcgtattg tttgaaaaag
     1441 gctatcaggc gggcgacgaa atcggcttct accggctgct gttctacatc ggcgcagcca
     1501 caggcttcct gctgctcctg ctcgtcccca aattgaacaa aatgctcgaa ggcacagact
     1561 aagtccttcc ccgatgcgcc gatgccgtct gaacccttca gacggcattt ttcccgcata
     1621 atgaaaccaa accgtttcca cccgacagga caggctcccg cccaaccgga aggcagcctg
     1681 ccgattgtca tttaaatatc tgactgattc cgtctgtttg caagggaaat agccgtttat
     1741 ttccgcttgg gcggaaaccg gttctattga gtaaaaggca ttttatccga ctaaattagt
     1801 gctgcatcaa cggaatatat agtggattaa
//
LOCUS       DAWFJW010000194         1732 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFJW010000194 DAWFJW010000000
VERSION     DAWFJW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.194"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            354..1658
                     /locus_tag="WGR19_002055"
     CDS             354..1658
                     /locus_tag="WGR19_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0799507.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          356 a          433 c          508 g          435 t
ORIGIN      
        1 ttaaaaattt ttaatatatt aatcaataaa ttaattttat aaaataaaaa tattgtcaac
       61 aatattttgc cttatcgccc aaacctctgt atattttcct acagtaaatt gttgacaatc
      121 catacgccca catatgcgcc gcctaaggat aaatcctccc gccggacaac gggtgcaagg
      181 gattggatgc gatatttcca tattcaaaca agggatttgt ttcacgcaca gggcggcaca
      241 tcggcaaaat ccccgcgccg ccccggtcca gcagggcttg cgtccctccc ggacaagccc
      301 cgaccccgcc tttccgaaag acgggctcaa ccattaagga aactttaatc aaaatgaaaa
      361 aacacatatg ggcggcatct ttgctgccgg catccctatc ggcagaacct ttaaactggt
      421 ggaagcctta ttccgccgtc aattcgggcg ataccgcctg ggtgatgact gcggctgcct
      481 tggtactgtt gatgacgctt cccgggctgg ctttattcta cggcggtatg gtgcggaaaa
      541 aaaacctgct ctcgacgatg atgcacagct tttccatcgc gacattggtg ggcatccttt
      601 gggtcgccgt cggctattct ttagcgttca cgccgggaaa tgcctttatc ggcggtttgg
      661 ggcgtgtatt tttaagcggg atgcagatag acgctaccgc acagatgctg accgtgtcgc
      721 ccaatgcgcc gactgttccc gaaccggtat ttatgttttt tcagatgacg tttgccatta
      781 tttcgaccgc cattattacc ggcgcgtttg ccgaacggat gaaatattcg gcaatgatgc
      841 tgttttcggg catatggttt ttattggttt atgtgccggg cgcgcattgg gtgtggggcg
      901 gcggctttat gagcaagggc ggcgtattgg attatgccgg cggtacggtg gtgcacatca
      961 atgccggtat cgcgggactc gtcgccgcct tggttttggg caggcgcata ggctacgggc
     1021 gcgaggcgat gcctccgcac aatatggcga tgacactgat cggcgcggca atgttgtggt
     1081 tcggctggtt cggctttaac gccggatccg cacttgcggc agacgcggcg gcgggtatgg
     1141 cgatggcggt aacgcaggtg tcggccgtat tcggcgcggc aggctggctt gcctgcgaaa
     1201 aaatagcggg acacaaacct tccgctttgg ggctggcttc cggcgcggtt tccggtctgg
     1261 tcggcatcac ccctgccgcc ggctttaccg gcccgtcggg cgcggccgcc atcggtatat
     1321 tgactgccgc cgcgtgcttt gtgtccgtca ccgtcgtcaa acacaaattg cgttacgatg
     1381 attctttgga cgctttcggc atacacggat tcggcgggct ggtgggcgga atattgaccg
     1441 gcatcttttt cgacaaccgc attttcggcg gggatgcggc agtttggcag cagttgtgga
     1501 tacaggtaaa agacgggttc gttatggcgg catacagcgg gctgatgagt tgggcgattt
     1561 tgaaggtcgt ggggaaaatc tgcggcggcc tgcgcgtcgg caaggatgtc gaacgcgaag
     1621 gtttggatct gaatatccac ggcgaacgcg tggaataagg gcggttatgc cgtctgaagc
     1681 ctgaaaatcg ggtttcagac ggcatttttc acgtttgccg ccgatggata aa
//
LOCUS       DAWFJW010000195         1729 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFJW010000195 DAWFJW010000000
VERSION     DAWFJW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.195"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(114..770)
                     /locus_tag="WGR19_002056"
     CDS             complement(114..770)
                     /locus_tag="WGR19_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0799508.1"
                     /translation="MKNKSKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            835..1725
                     /locus_tag="WGR19_002057"
     CDS             835..1725
                     /locus_tag="WGR19_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0799509.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMVGCGLGTDKATVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGVWLHGAAADVIKESAGIAAGL
                     LAGEISPAARWLRNRITESM"
BASE COUNT          381 a          414 c          575 g          359 t
ORIGIN      
        1 ggtttgggac ggaaaaacgg cctgaagccg tttcgggcat tcagaccgtt tgcgcggaga
       61 ggggatgccg tccgaagggc gaaaagggct tcagacggca ttgatgtcgg gtttcaggac
      121 aggagcagga tggcggctgc gacaagcgcg gcaaccgata atgcggcggc aagcgcggtt
      181 ttgccggcaa agcggattga ggttttgcct tcgatgtatt tgaagccggt tatcatcggg
      241 aggacgaggt ttttcttttt gaacacgcgg tatgcggcga cggcggcgat atgaatggcg
      301 gaaaaaacgg cgagcagctt gaaaaagttg aggtggattt tccgcataag gctgcccgta
      361 tgctcggaaa ccaaatggtt gaggtagccg ttggtgctga aggtgttttc atcggcggca
      421 aaaagccctg tgccgacttg aaatgagacg gcggccaaaa gcgcgacgac catcagtgcg
      481 cccaaggggt tgtgtccggg ttggacgtgt tcgggaatac cgtttttcat atactctctg
      541 atacccgacc atccgcggac gaaacgggag aaacgggcgg tatcgctgcc ccaaatgccc
      601 cagcagaggc ggaatacgag caggaaaagg ataaacagcc cgacgcgcgt gtgccattgc
      661 agcatatcgc cgccggtttt cgcgctatac cacataaagg gtagggatgc ggcaagcagc
      721 cagtggaaaa ggcgggtggg gaagtcccag actttgcttt tgtttttcat aatcggtttc
      781 cggcggtaga atcggtttgt tttcgagcct taattttaac cgattggagg ggaaatgttt
      841 cccgttttcc atctttcagg cgaaagccgc cgccggatgc ttcaggcggc atcgcgtttt
      901 ccctatgttt tcaaagcccg tgcggaagat tcgcacaaag ggactttcgg cacgctcgcc
      961 gtagtcggcg gatcggcagg gatgagcggc gcgcccgtat tggcggcatc ggcggcgatg
     1021 tatctcggct gcggcaaggt gtgggcgggt ttcaatcagg atgcgcttcc gtttgccgtc
     1081 attgccggtt ttcccgaaat tatgttggac acggcagacg gtttgaccaa gcgtcaaggc
     1141 ataaacgctt ggatggtagg ttgcggcctg ggtacggata aagcgacggt cggaacgctt
     1201 gccggaattt tggcggaaca cacggacaag cccgtccttt tggatgcgga cgcgctgaac
     1261 atattatcaa ccgatgccga aacccgaaat ctggcgcgcg ggtgtaaaaa cctgatttta
     1321 acgccacacc ccgccgaagc cgcgcgcctg cttggaacga cggttgcaca ggttcaggcg
     1381 gatcggacgg cggcagtgag gaagataagg gcaattttcg gcgcaaccgt ggttttaaag
     1441 gggcacaaaa cattggttgc ctcacccgat acggaaatct atgtcaacga aagcggcaac
     1501 gcgggattgg caacggcggg cagtggcgac gtattgggcg gcatcatcgg cagtctgctc
     1561 gcacagggcg tgccggtttt tgaagccgcc tgcgcgggcg tgtggctgca cggcgcggcg
     1621 gcggatgtca taaaagaatc ggcaggcatt gcggcggggc tgttggctgg cgaaatctcc
     1681 ccggcggcaa ggtggctgcg caaccggata actgaaagta tgtaagaag
//
LOCUS       DAWFJW010000196         1714 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFJW010000196 DAWFJW010000000
VERSION     DAWFJW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.196"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            372..1628
                     /locus_tag="WGR19_002058"
     CDS             372..1628
                     /locus_tag="WGR19_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0799510.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
BASE COUNT          550 a          324 c          455 g          385 t
ORIGIN      
        1 taaagtttga gctaagaggc cgtctaaaaa cagaaaaacc gtttcagacg gtctttgttt
       61 aacgccaccg atccagcggg ttacaaagcg cagtcaatgc cgctgcgcct tatgcctccg
      121 aagcaatagg cagaacattt ggacacggtg aaaacaaaaa cgaaaccgcc caagccgtcg
      181 gacatttcct tttaggagca gctattgccc gcgtcaacgg tggtaatttt gctgccggcg
      241 gctcggcagc agttgcagct gaaaaggcgg cggaacatct tgcccaacag tataacgacg
      301 gtaaaaccgc aatcgatccg caaacaggcg agttcaatgc caacctgctg ccggaacata
      361 tcaaagagga aatcaaatca aagagcgggg tgattgcatc gctgacgggc gcggccgtgg
      421 gcggcacgcc ggtagatgcg caaaccggag gtgcggtcgg acagaatgcg gtggaaaaca
      481 acctctatct gacatcggaa gccttaaaga aggacaagca gacagctcgt aaaatttatt
      541 ccgtcataaa agagcaagtc aagcatgaat gcagttccac aggaagaatt accgaatgtc
      601 gtcaaaatat aggacgcatt atcgaattta cccaagacaa acgctttgac agtaggttta
      661 aggacttaaa aaaagaatcc ttatattacc taaataaaca tcctgattta gtagcctctt
      721 atttgaaggc tgaatacgaa aagctggata gggaagacaa aagtatcctg caccgctaca
      781 tctcacccgg ggctgaaatc gtttcgggca gtttgggggt tgttctttca ggagtagccg
      841 gaggcggatc ttgtgccgag actttcggct taggctgtgc cgccgctttg gttggtgtaa
      901 cgtcttccta cgatcatgtc attactggga cgaaaaactt cggaaaaaaa gccagcgagc
      961 aacgaccgac gattgcggtt caggccttga agcagttggg gctgtcggag caggctgcgg
     1021 aatatgttca gttctctata gatttgttca gtgtgggtaa atcggggggc ggtataccta
     1081 aggctaagcc tgtgtttgat gcgaaaccga gatgggaggt tgataggaag cttaataaat
     1141 tgacaactcg tgagcaggtg gagaaaaatg ttcaggaaac gagaagaagg agtcagagta
     1201 gtcagtttaa agcccatgcg caacgagaat gggaaaataa aacagggtta gattttaatc
     1261 attttatagg tggtgatatc aataagaaag gcacagtaac aggagggcat agtctaaccc
     1321 gtggtgatgt acgggtgata caacaaacct cggcacctga taaacatggg gtttatcaag
     1381 cgacagtgga aattaaaaag cctgatggaa gttgggaggt gaaaacgaaa aaaggtggga
     1441 aagtgatgac caagcacacc atgttcccaa aagattggga tgaggctaga attagggctg
     1501 aagttacttc ggcttgggaa agtagaataa tgcttaagga taataaatgg cagggtacaa
     1561 gtaaatcggg tattaaaata gaaggattta ccgaacctaa tagaacagca tatcccattt
     1621 atgaatagta atatttatga aaaattagga gattaatgat gaaaagaatt aagtgctttt
     1681 gtgataaatt tccatcagga gatacattta gaat
//
LOCUS       DAWFJW010000197         1691 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFJW010000197 DAWFJW010000000
VERSION     DAWFJW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.197"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            144..1154
                     /gene="dusB"
                     /locus_tag="WGR19_002059"
     CDS             144..1154
                     /gene="dusB"
                     /locus_tag="WGR19_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0799511.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCREFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIGEMPDGEQARREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
     gene            1184..1423
                     /locus_tag="WGR19_002060"
     CDS             1184..1423
                     /locus_tag="WGR19_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0799512.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            1426..>1691
                     /locus_tag="WGR19_002061"
     CDS             1426..>1691
                     /locus_tag="WGR19_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0799513.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQA"
BASE COUNT          390 a          492 c          468 g          341 t
ORIGIN      
        1 cctcctgtga gtggcggggc agggaatctg tgtgcggatt atgccataaa acggtgcccg
       61 ccccaatcgc gggcgcgtcc ggagattgga aatcctgctt aaaaaatgta caatgtcgca
      121 cttttttgaa acgcggaccc attatgcaca tcggcggcta ttttatcgac aaccccatcg
      181 cacttgcgcc gatggcgggc attaccgaca aacccttccg ccgcctttgc cgcgagtttg
      241 gcgcgggttg ggcagtgtgc gaaatgctga ccagcgaccc gacgctcaga aatactagaa
      301 aaaccttgca ccgcagcgat tttgccgatg aaggcggcat cgttgccgtg cagattgccg
      361 gcagcgatcc gcagcagatg gcggatgcgg cgcgttacaa cgtcagcctc ggggcgcagc
      421 ttatcgacat caatatgggc tgtcccgcca aaaaagtgtg caacgtccaa gccggcagcg
      481 cgctgatgca gaacgagccg ctggttgccg ccattttgga agccgtcgtc cgtgcggcag
      541 gcgtacccgt taccctcaaa acccgtttgg gctggcacga agaccatcaa aacctgcccg
      601 tcatcgccaa aatcgccgaa gattgcggca tcgccgccct tgccgtccac ggacgcacgc
      661 gcacgcaaat gtacaaaggc gaggcgcgtt acgaactcat cgccgaaacc aaatgccgtc
      721 tgaacatccc ggtctgggtc aacggcgaca tcacttcgcc gcaaaaagcc gccgccgtcc
      781 tcaaacaaac cgccgccgac ggcatcatga tagggcgcgg cgcgcaaggc agaccgtggt
      841 tcttccgcga tttgaaacat tacgccgaac acggtgtttt accgcctgcc ttgagtttgg
      901 cagaatgcgc cgccgctatt ttgaaccaca tccgcgccat acacgcgttt tacggcgata
      961 gtgcaggcgt acgcatcgca cgcaaacaca taggttggta catcggcgaa atgcccgacg
     1021 gcgaacaggc gcggcgtgaa atcaaccgct tggacagtgc ggcggcgcaa tacgacatgc
     1081 ttgcaggtta tcttgaaaga cttgccggaa aaaccgaccg ttgggcgtgc ggctaccgca
     1141 caaatgcctt ctgaacactt gattatcctt tgaaagtgca atcatgcccc atacccttcc
     1201 cgatatttcc caatgtatca gacaaaattt ggaacaatat ttcaaagacc tgaacggtac
     1261 cgaaccttgc ggcgtgtacg atatggtatt gcatcaggtg gaaaagccgc tgctggtgtg
     1321 cgtgatggag caatgcggcg gcaaccagtc caaagcctcc gtcatgttgg gactgaaccg
     1381 caatactttg cgtaaaaaac tgattcaaca cggtttgctg tgaatatgtc ggcaaccgtc
     1441 cgtatcttag gtatcgaccc gggcagccgc gtaacgggtt tcggcatcat cgatgtcagg
     1501 gggcgcgatc atttttacgt cgcctccggc tgcatcaaaa cgcctcccga tgcgcctctg
     1561 gcagacagga ttgccgtgat tgtgcggcac atcggcgaag tcgttgccgt ttacaagccg
     1621 cagcaggcgg cagtggaaca ggtgttcgtc aacgtcaatc ctgcatcgac gctgatgctc
     1681 ggtcaggcta g
//
LOCUS       DAWFJW010000198         1607 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFJW010000198 DAWFJW010000000
VERSION     DAWFJW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.198"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(242..1237)
                     /gene="porB"
                     /locus_tag="WGR19_002062"
     CDS             complement(242..1237)
                     /gene="porB"
                     /locus_tag="WGR19_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0799514.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGKYNSESYHAGFNYKNGGFFVQYGGAYKRHVRVDENVNIEKYQIHRLVS
                     GYDNDALHASVAVQQQDAKLVEDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSF
                     DDADLSNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          425 a          365 c          393 g          424 t
ORIGIN      
        1 attaaagttt caaaatttat tctaaatagc tgaaactcaa cgcactagat tcccgcctgc
       61 gcgggaatga cggcatattt tgacattgaa taaaaaagac taaaaacagg aaaaagccaa
      121 acagaaaaca gcgaaacaga aaacagccaa acagaaaaca gccaaacaga aaacagccaa
      181 acagaaaaca gaaaaaagcc tgtctggcga caggcttttt gttgatacca atctttgcag
      241 attagaattt gtggcgcaga ccgacaccgc cggcagtcga tacgaatttg ttttcgcctt
      301 taccttcttg caaccaaccg gcagaaacca aggcagaagt gcgtttggag aagtcgtatt
      361 cagcaccgac aaccacttgg tcgtaatcgt tgcttaagtc tgcatcatca aacgagcctt
      421 tgaagccgtg ggcgtaagaa acgcggggcg ttacgttgcc gaagcggtat gccaaggtag
      481 cggcaacttc ggtttgagag ttgtgcgaat aattgtcttc aaccaatttg gcatcttgtt
      541 gctgtacggc tacggaagcg tgcagggcat cattgtcgta accgctgacc aaacggtgaa
      601 tctggtattt ctcaatattc acgttctcat ccacccgcac atgtctttta taggcaccgc
      661 catattgcac gaagaagccg ccgtttttgt agttgaagcc ggcgtggtaa gattcgctgt
      721 tatatttgcc tgcattgtcg ttaagcgcgt attgtacgct gccgctgagg ccggcaaatt
      781 cgggagaatc gtagcgtacg gaaatgaggc gtgcttcggg ttcggcaatt ttgtttacac
      841 ccaaatagtc gcttttgcta tcccaaggat tgatgtcgcc agtgtctttc aggacgctgt
      901 tcaaacggcc gacgcgcaat ttaccgaagc cgcctttcaa accgatgaag gattggcggt
      961 tgccccaacc ggagtcagta ccggcgatag atgccttttg ctcaacctgc caaatggctt
     1021 tcagaccgtt accgaggtct tcttggcctt tgaagccgat ttttgaaccc aaatcaacga
     1081 tgccggtacc ggtttcaacg ctaaccacct gacctccatt gtgctctaca gagcgggaag
     1141 tttctacgcc ggctttgatg gtgccgtaca gggtaacgtc agccattgct gcaacaggaa
     1201 gggctgccaa agtcagggca atcagggatt ttttcattgc tgtattcctt ttttggttaa
     1261 gaaatttaag caggccgggc tttccaagcc gcttagcttt gcatttaccg ccgacgtttt
     1321 cggcgattcc atgtctgaaa ctatagatgt tcctgtctga aaaaacaaac ttctgcctgt
     1381 ttttgtgtgg aattcgatgt gtattttgaa gggcggatag agatattgtg ggtatttttt
     1441 gttttataac atatggttat ttaaattttt taagatttgc atttttacaa cacttactcg
     1501 ggagggtatt ggagggcatt gcaaaccggg ggttataaag acggcaaaaa acgcgcacgc
     1561 ggtttctttc ggaacgggcg gatgcaaaac ccgcccctgc ggcagga
//
LOCUS       DAWFJW010000199         1598 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFJW010000199 DAWFJW010000000
VERSION     DAWFJW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.199"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..187
                     /locus_tag="WGR19_002063"
     CDS             <1..187
                     /locus_tag="WGR19_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799515.1"
                     /translation="ADRQNHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFN
                     FGTPSQQLKILRDWCGI"
     gene            1045..1157
                     /locus_tag="WGR19_002064"
                     /pseudo
     CDS             1045..1157
                     /locus_tag="WGR19_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1186..>1598
                     /locus_tag="WGR19_002065"
     CDS             1186..>1598
                     /locus_tag="WGR19_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0799516.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGN"
BASE COUNT          411 a          364 c          422 g          401 t
ORIGIN      
        1 tgcagaccgt cagaaccaca ttaacggcat tgagaacttt tggaatcagg caaaacgcgt
       61 cttgcgaaaa tacaacggaa tcgatcgcaa atctttcccg ctgttcttga aagaatgcga
      121 atttcgattt aacttcggca caccgtctca acagcttaaa atcctgcggg attggtgtgg
      181 gatttagggc taatctagta cagcccctta gatttaattc actatacctg cgtaatggta
      241 tcgggtactc atcatcaacc tgccttgcat tcggttgtgt tgtccggcaa ctgtccggtt
      301 gtgtcgatgg gttgccctgc cgctccctgt gctgcgcaac ggttgtgtgc cttgggtggg
      361 cgcgggtggc ggccgggcgg tttgttgcta tgatacggga gttccgatat acaagggtgg
      421 gcttcagccc accaatcaac gaccgaaaaa gaaaaaacgt tgggctgaag cccactctac
      481 gcaattacgc aactgttatc gtggggatga cgggatttta ggtttttgtt tttctgtttt
      541 tgcgggaagg atgggtttta ggttgcggtc atttatcgga aaagcagaaa tcgctccgcc
      601 gtcattcccg cgaaagcggg aatctgaaca cgtccgtagg gaaacctata tcccgtcatt
      661 cccacgaaag tgggaatctg aacacgtccg tagggaaacc tatatcccgt cattcccacg
      721 aaagtgggaa tctagaacct ctgaattttc agacaacctt tgaatattgc cgccgtcccg
      781 cgttctagat tcccgcctgc gcgggaatga cgattcatca gtttcccgaa acgcccgaaa
      841 aaccgaaacc gagcaaaccg gattcccgcc tgcgcgggaa tgacgattta taagtttccc
      901 gaaacaccca aaaaaaccga aatccgacag gcaggatttt tgctttcgtg gggatgacgg
      961 gattttaagt tacggtcatt tatcggaaaa gcagaaactg ctccgccgtt attccagcga
     1021 aagcgggaat ctgaacacgt ccgcacggaa acctatagtg gattaacaaa aatcaggaca
     1081 aggcgacgaa gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtactggt
     1141 ttaaatttaa tccactataa tcctgttgct tatggaaaaa tcaaagtgca gagttggaaa
     1201 gagaggcgcg atttcaatat tgtaaagcag gatttggatt tttcctgcgg ggcggcttcg
     1261 gtggcgacgc ttttgaacaa tttttacggg caaaagctga cggaagagga cgtgttaaaa
     1321 aaactggata aggagcagat gcccgcgtcg tttgaggata tgcggcgcat tatgcctgat
     1381 ttgggttttg aagcgaaagg ctatgccctg tctttcgagc agcttgcaca gttgcaaatc
     1441 cccgtcatcg tgtatctgaa ataccgcaag gatgatcatt tctcggtatt gcgcggtata
     1501 gacggcaata cggttttgct tgccgacccg tcgctgggtc atgtttcgat gagcagggcg
     1561 cagtttttgg atgcttggca aacccgtgag ggaaattt
//
LOCUS       DAWFJW010000200         1592 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFJW010000200 DAWFJW010000000
VERSION     DAWFJW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.200"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            59..1432
                     /locus_tag="WGR19_002066"
     CDS             59..1432
                     /locus_tag="WGR19_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0799517.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          416 a          520 c          385 g          271 t
ORIGIN      
        1 cccatcctat cggtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcagtc
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccaccgtcg gacggctgct
      481 cgaccacgtg aaacagaaaa acatccattt gaacaaagtc gaaatcgtcg ttttggacga
      541 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      601 gccccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gagcagcaca tcatcgccgt cgataccatt cagaagcgca acctgctcga
      781 acggctgatt gtcgatttgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcaacctgcg
      961 cgtcctcgtc gccaccgata tcgccgcgcg ggggctggat attgccgaac tacccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccaag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaaa
     1321 tgcggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat aaccctgaaa
     1441 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaacca gactaacgca
     1501 ttgggcgcaa ccgcccgcac aggataaatt tctgccgcaa acagtttcag acggcatttg
     1561 ccgcctgtac aatatagtgg attaacaaaa at
//
LOCUS       DAWFJW010000201         1520 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFJW010000201 DAWFJW010000000
VERSION     DAWFJW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.201"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            40..1230
                     /locus_tag="WGR19_002067"
     CDS             40..1230
                     /locus_tag="WGR19_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA"
                     /protein_id="HEZ0799518.1"
                     /translation="MTVGYGFSASGDYSQSKIRANHASVTEQSGIYAGEDGYQIKVGN
                     HTDLKGGIITSTQSAEDKGKNRFSTGTLAGSDIQNYSQYEGKSFGLGASASISGKTLG
                     QGAQNKPQNKHLTSVADKNGASSSVGYGSDSDSQSSITKSGINTQNIQITDEAAQIRL
                     TGKTAAQTKADIDTNVTTDTAERHSGSLKNVFDKDRVQSELDLQRTVSQDFSKNVQQA
                     NTEINQHLDKLKADKEAAETAAAEALANGDMETAKHKAHEAQDAAAKADNWQQGKVIL
                     NMLASGLAAPTQSGAGIAAATASPAVSYAIGQHFKDLAGQNANGKLTASQETAHVLAH
                     AVLGAAVAAAGGNNAPAGALSAGGSEAAAPYISKWLYGKEKGSDLTAEEKETETFAKF
                     PKIP"
     gene            1261..>1520
                     /locus_tag="WGR19_002068"
     CDS             1261..>1520
                     /locus_tag="WGR19_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799519.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPACPLLSMFKAVLLGQWHSLSDPELEHSLITRID"
BASE COUNT          506 a          411 c          352 g          251 t
ORIGIN      
        1 gaaacctatc aaagcaaaca acaaaacgcc agtgcacaag tgaccgtcgg ttatggcttc
       61 agtgccagtg gcgattacag ccaaagcaaa atccgagcca atcatgcttc ggtaaccgag
      121 caaagcggta tttatgccgg agaagacggc tatcaaatca aggtcggaaa ccatacagac
      181 ctcaaaggcg gcatcatcac cagtacccaa agcgcagaag acaagggtaa aaaccgattc
      241 agcacaggca cactcgccgg cagtgatatt caaaattaca gccaatacga aggaaaaagt
      301 tttggattgg gcgcaagtgc gtccataagc ggcaaaacac tgggacaggg cgcacaaaat
      361 aagcctcaaa acaaacacct gacaagcgtg gccgataaaa acggcgcaag ttcatcagta
      421 ggctacggca gcgacagcga cagccaaagc agcatcacaa aaagcggcat caatacccaa
      481 aacattcaaa tcaccgacga agccgcacaa atccggctga caggaaaaac agcggcacaa
      541 accaaagccg atattgatac aaacgtaacc acagacaccg ccgaacgaca ttcgggcagc
      601 ctgaaaaacg tatttgacaa agatagagtg caaagtgaac tggatttaca aagaaccgtc
      661 agccaagatt ttagtaaaaa tgttcaacaa gccaataccg agattaacca acatttagac
      721 aaactcaaag cagacaaaga agcagccgaa acagcagcag ccgaggcatt agccaatggc
      781 gatatggaaa ctgccaaaca caaagcccat gaagctcaag atgcggcagc aaaagcagat
      841 aattggcaac aaggcaaagt cattctcaac atgttagcct caggtttagc tgcgccgacc
      901 caaagcggag cgggcatcgc tgcggctacc gcatcgccag ccgtatcgta tgcgattgga
      961 cagcacttta aagatttagc cggtcaaaac gcgaatggca aactaaccgc cagtcaagaa
     1021 accgctcacg ttcttgccca cgcggtatta ggagcagcgg ttgcagcagc aggaggcaac
     1081 aatgccccgg cgggagcatt gagtgcgggc gggtcggaag cggctgcgcc ttacatcagc
     1141 aaatggttat atggcaaaga aaaaggaagt gacttaacgg cggaagagaa agagactgag
     1201 acctttgcaa aattccccaa aatcccctaa attcccacca agacatttag gagatttctc
     1261 atgagcacct tcttccagca aaccgcccaa gccatgattg ccaaacacat cgaccgtttc
     1321 ccgctattga agttggacca ggtgattgat tggcagccga tcgagcagta cctgaaccgt
     1381 caaaaaaccc gttacctccg agaccaccgc ggtcgtcccg cctgtcccct gttgtccatg
     1441 ttcaaagccg tcctgctcgg acaatggcac agtctctccg atcccgaact cgaacacagc
     1501 ctcatcaccc gcatcgattt
//
LOCUS       DAWFJW010000202         1519 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFJW010000202 DAWFJW010000000
VERSION     DAWFJW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.202"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            1..213
                     /locus_tag="WGR19_002069"
                     /pseudo
     CDS             1..213
                     /locus_tag="WGR19_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(141..725)
                     /locus_tag="WGR19_002070"
     CDS             complement(141..725)
                     /locus_tag="WGR19_002070"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEZ0799520.1"
                     /translation="MASIILNYLEKDGRPVIHGMGYISEWKINNGKVFYNISDTILNE
                     LLLKSGRSMFLLMDWSVFNSLPGKYCGILYKLCKDYAGIGKTPYFTVQEFRHYLGLND
                     KEYEEFRALKAKIIKPAIEHINNSENVDIKIIPQLNQNMADEDIETAFSESKIEPLAT
                     LIKFLETKTPRSSSGLERLCHCPSRTALNMDNSG"
     gene            complement(1160..>1519)
                     /locus_tag="WGR19_002071"
     CDS             complement(1160..>1519)
                     /locus_tag="WGR19_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799521.1"
                     /translation="NPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVFWDGNK
                     RTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNCISGID
                     YFGADEDIDFPDSPNID"
BASE COUNT          396 a          347 c          286 g          490 t
ORIGIN      
        1 accgcacaag ccatgattgc caaacacatc gaccgtttcc cgctattgaa gttggaccag
       61 gtgattgatt ggcagccgat cgaacaatac ctgaatcgtc aaagaacccg ttaccttaga
      121 gaccaccgcg gccgtcccgc ctacccgctg ttgtccatgt tcaaagccgt tctgctcgga
      181 caatggcaca gcctctccaa tcccgaactc gaacgtggtg ttttggtttc cagaaattta
      241 atcagtgtgg ctagcggctc tattttcgat tcagaaaagg ctgtttctat atcctcgtct
      301 gccatgttct ggtttaattg cggtataatt ttgatgtcca cattttcact gttgttgata
      361 tgctctattg ccggtttaat tattttggcc ttcaacgctc taaattcttc gtattctttg
      421 tcattcaatc ccaaatagtg tctaaattct tgaacggtaa aatagggggt tttgccgatg
      481 cctgcgtaat ccttgcacaa tttgtatagg attccgcaat attttcccgg taagctattg
      541 aacacactcc aatccatcag caaaaacatg cttcttccgc ttttcaatag taactcatta
      601 agaatggtgt ctgatatatt gtagaaaact ttgccattgt tgatcttcca ctctgaaata
      661 tagcccatac cgtgtatgac gggacggccg tctttctcca aataattcaa aatgattgat
      721 gccattcgta gagcatccaa gtctttttta gttgttttct ttgaagttga tatccaatac
      781 aacgttccag atctaagata tctatcgcaa caaaaccgtt gtcatcataa gtaccacctt
      841 attcaaaatg tgttttaatc ttctgcaaaa aaagatacca atacttgtat tgcatttcag
      901 tcaaggaatg gcccatgtgg attaaggatt gcggtgtttt ttgtttatca atatggtttg
      961 atgtcatttt gatttccatt tcatgtggct actatatctc tctattatgc actaatacac
     1021 agagtttctt gatgttagaa cgaatacggc gtccgtgtcg gcgtgaaatt ctgattttcc
     1081 ttatacgaaa ccgcaaacct ttattacatc tcctgaataa tttaggatac tttttaggta
     1141 ttgaaaaaca ccttggtttt tagtctatgt tcggtgagtc ggggaaatct atatcttcgt
     1201 ccgcgccgaa atagtctatg cccgatatac aattttgata cacaaacttg gaaatatcgg
     1261 tatcgtcacc cgagcgatag aatgcggaca gtttttcatt gaattgcggc atctgcattt
     1321 cggagatttc caagatgcca cggcccctct ccatcatcag accattggca aataaggttg
     1381 ccgttcgttt gttgccgtcc caaaaaacct gctgccgcat acaataaagc ataaaacgga
     1441 cggctgcctc cgtcgtcgaa ccgctttgca gtccgatatt ttgtaacacg cgagccactt
     1501 caatttcctt caccggatt
//
LOCUS       DAWFJW010000203         1488 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFJW010000203 DAWFJW010000000
VERSION     DAWFJW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.203"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(160..1407)
                     /locus_tag="WGR19_002072"
     CDS             complement(160..1407)
                     /locus_tag="WGR19_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0799522.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          331 a          398 c          424 g          335 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata cccaaacaca gtcaaacaaa tttatatgcc
       61 ccatcccttc cgaataattt gaaaacacag ccgccaaaaa caaaaatgcc gtctgaaaac
      121 ctttcagacg gcatttccaa cttgatttca ggcagaaagt cagaacgcga tatagctgtt
      181 cggattaacc ggtttgccgt tttgccgcac ctcgaaatga agctgcgttc tggaagcgtc
      241 ggtattgccc atcaaagcga cctgctgccc gcgtttgacc tgctggcctt cgccgaccag
      301 caatttttgg ttgtgcccgt atgcggtcag gaaggaagaa ttatgctgga tgatgaccag
      361 attgccgtat cccctcaaac cggaacctgc ataaaccact ttgccgtcag ccgccgccaa
      421 aacgggctgt cccgcattac cggcaatatc gacacccttg ttgttgccgc cgaaatcggc
      481 aaccacttta ccttgcgtcg gacgctgcca aacaatgccg ccgaccgaac gcgtgccgga
      541 aggcgaagcg gcaggagatt gcggggcggg cgcgggaacc gctttatttt ccgcagcggg
      601 cgcggcaggt tgcggcgcgg actgcacagg cggttgcgcg gcgggtttca caggggtttg
      661 cacggcagcc ggtacggcgg gcctgctttc tacggctgag gttttcggtg cggcatatcc
      721 tgccggtttg actttaacaa tctgaccgat gctcaacata ttgtcggtca tgccgttcca
      781 cgcacggaaa tcgtcttgag agatatggta gcgtttggaa atgttgtaca ccgtgtcgcc
      841 gcgcacaata gtatgcgtcg ccgcgttgat gtcgacgggt gcggactgta cgggcggttg
      901 cgcggcagcc ggtacggcgg gcctgctttt tacggctgcg gctttcggtg cggcatatcc
      961 tgccggtttg actttaacaa tctgaccgat gctcaacgta ttgtcggtca tgccgttcca
     1021 cgcacggaaa tcgtcttgag agatatggta gcgtttggaa atgttgtaca ccgtgtcgcc
     1081 gcgcacaata gtatgcgtcg ccgcgttgat gtcgacgggt gcgtaagaag gaatgtatgt
     1141 acccgaaacg gcaggtgcag acggcggaac ataagcagga ggcgtataaa ccggcgcgct
     1201 ttgcaccggc ggcacataag gcgcatcgcc ggcaggagcc gggctgtacg gcgttgctcc
     1261 ataggggttg ttgtaaactg ccgaagacgg cgcgtcctgc atacctgaat tgcctgcaat
     1321 gacgggggca ggctgctggg tagcacaacc gcccaacagt gcggcaaagg aggcgcaagc
     1381 cgccaaaagt gtcgtttgtt tcaacataag ataaccttca tgttccgata tatagcctga
     1441 atgcggtata tcataataaa aatgcgcgtt cttctcaagc gcaaagcc
//
LOCUS       DAWFJW010000204         1464 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWFJW010000204 DAWFJW010000000
VERSION     DAWFJW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.204"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            21..401
                     /locus_tag="WGR19_002073"
     CDS             21..401
                     /locus_tag="WGR19_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mAFB alternative"
                     /protein_id="HEZ0799523.1"
                     /translation="MQTVDGEMAGGNRPPKSITSNSKADASTQPSLQAQLIGEQISSG
                     HAYNKHVIRQQEFTDLNINSPADFARHIENIVSHPTNMKELPRGRTAYWDDKTGTIVI
                     RDKNSDDGGTAFRPTSGKKYYDDL"
     gene            411..617
                     /locus_tag="WGR19_002074"
     CDS             411..617
                     /locus_tag="WGR19_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799524.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            749..1000
                     /locus_tag="WGR19_002075"
     CDS             749..1000
                     /locus_tag="WGR19_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799525.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            997..1368
                     /locus_tag="WGR19_002076"
     CDS             997..1368
                     /locus_tag="WGR19_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0799526.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          533 a          234 c          295 g          402 t
ORIGIN      
        1 aaaaacctgc tgcccgccat atgcagactg tagatgggga gatggcaggg gggaatagac
       61 cgcctaaatc tataacgtcc aacagcaaag cagatgcttc cacacaaccg tctttacaag
      121 cgcaactaat tggagaacaa attagtagtg ggcatgctta taacaagcat gtcataagac
      181 aacaagaatt tacggattta aatatcaatt caccagcaga ttttgctcgg catattgaaa
      241 atattgttag ccatccaaca aatatgaaag agttacctcg cggtagaact gcgtattggg
      301 atgataaaac agggacaata gttatccgag ataaaaattc tgacgatgga ggtacagcat
      361 ttagaccaac atcaggtaaa aaatattatg atgatttata ggaaaaagcc atgaatatac
      421 tatccataaa taatcaaaac tcaactattt cactaactca agatgaagtt tttgttttac
      481 gagctatctt gaatgagata tatgcgggcg tatgtgtaga ttcaagagaa tttgaaaatg
      541 tatctggtgt tagaaaacat gaagtagata atttacaaca acagtttgct ggaatttata
      601 aaaaaatgac aacttaacaa cccaaatttt tgtcagagcc tagtgcaaat tacaactatg
      661 attctattgt agccgaaatg aaagaaaaaa atcatgggtt ggcgacaggg ttgatgttgt
      721 taatatgcct gatggagcac ctactagtat ggataacacg cgtattatgg cagcacgtga
      781 agcaggagta aaagtggaag cgaatgttca taattttaat gaccgattat catcaaaaga
      841 gagaatcagg tttaagcatg atggtattga gcctcaaact tggggagaag ctatccagct
      901 acgaattaga aagcaagaaa cacaaaaagg agttccagaa gggtggagca aaagatttcc
      961 taacggaagt atttatgatg taaaggtact taggaaatga taaaacaaaa tagttttgtt
     1021 ccgtatcctg aagcaatgct tcctaaagga tttaaatatc cgcaaagtta tttaaaatta
     1081 gctcaatcca ctcatgccat taactacgat gaacaatatt cttttccttg gtggtttgaa
     1141 aatgcagaaa gcaatatatc agaagtaatt gacatttatt ttgaaataac tggcattcca
     1201 aacctattac cttttgctag aaaccaagag tgggctgcct gttttgatat ttcagataaa
     1261 tcaggtaatc ctaaaattat agtagttaat ttagataata caaaatatta cgagactttt
     1321 gaaaattttg atacttggct aaaagaagct gaaaatgatg gttggtagca gccgtaggtc
     1381 ggattctcga atccgacatt ttccaacagc ggcatttcgg aaacgataga cgcgtcaaat
     1441 atttttgtcg gatacaagta tccg
//
LOCUS       DAWFJW010000205         1386 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWFJW010000205 DAWFJW010000000
VERSION     DAWFJW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.205"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            41..373
                     /locus_tag="WGR19_002077"
     CDS             41..373
                     /locus_tag="WGR19_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0799527.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WGR19_002078"
     CDS             705..1214
                     /locus_tag="WGR19_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0799528.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          364 a          277 c          322 g          423 t
ORIGIN      
        1 accttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 accggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatatgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgccatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggt gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggcggaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcatca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tagcggatta acaaaaacca gtacggcgtt
     1261 gcctcgcctt gccgtactgg tttttgttaa tccgctatat tccgccatct ctaagattta
     1321 cagcaataca cgggtgattt aaggaatgcc cgaaccgtca ttcccgccac ttttcgtcat
     1381 tcccgc
//
LOCUS       DAWFJW010000206         1361 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWFJW010000206 DAWFJW010000000
VERSION     DAWFJW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.206"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            59..481
                     /locus_tag="WGR19_002079"
     CDS             59..481
                     /locus_tag="WGR19_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799529.1"
                     /translation="MKYMISFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYKFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            518..919
                     /locus_tag="WGR19_002080"
                     /pseudo
     CDS             518..919
                     /locus_tag="WGR19_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
     gene            1042..>1361
                     /locus_tag="WGR19_002081"
     CDS             1042..>1361
                     /locus_tag="WGR19_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799530.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAVTVATAQGYV
                     SKTKIKVGQTELRVTAATDKQLLKTISEGRDTTGKMTEQLFDSLAKQNGFKVLSGGKY
                     GANNG"
BASE COUNT          486 a          218 c          281 g          376 t
ORIGIN      
        1 gtaacataaa agatattgat aaatttatta gtgcaaacat aaaaaaatag gagttagtat
       61 gaaatatatg attagttttc taaaaaaaac atttgaatta atgagttggg tgttagtcat
      121 actaataatt gggacatttt atgactatta tcaaataagg caatatgctg aattagaaaa
      181 gaaatctata tcaaatatct tgctatatgc ccaaaaagaa aaatttcgct tagagagtaa
      241 agataaatac atgcgaggag gatatacaaa atataaattt attttttcag aatatagtaa
      301 tactactttt ttaaatttca taaatgacct gaaaaaagat aattatttac cacttgacgg
      361 ctatggacat ggttttctat gtagaaaagg agagtctata tcaatcaata tataccctga
      421 aattaataaa tttattttag tatggggata ccctgaaaat ctttgcgctg attctaatta
      481 agtaagcaag aataggttat agggaaataa aattcaaatg agaaaattga ataatcacga
      541 tgttcataaa cggtatcaag atcgtttaga agaggatgta gagttcacta tcaactatga
      601 gcttcctttg agttgtttgt ggtcaaccat caaagacttt tccagcgatt ttgaggaaaa
      661 aactgaagcg ttctttattc ttttcaaaga gctgctgcgc agaggtcatc tgaaactgca
      721 acgcgacggg taaattatcg ggcatacgcc cgaagaatgg gaacaaatat ttagggaagt
      781 atggcctgaa tatgaaatcg aacccaatcc acttcccggc tatgccccat ttgatattgg
      841 aatgtggctt acggtcgagg ctcctgccta cgccgtatgg atagatcccg aagacggtag
      901 cgaatactgg gcgggataaa ataccaatgt ttggaataaa tcccgtctga aaaacagctt
      961 tttcagacag gatttattcc aaacattggt gatatacaga gttttgtaca agcacagacc
     1021 gctgccgatt acctgtttgc tttgctgggt gtggttccgg gtatcggcga atcgatacag
     1081 gcctataaag tagcgaaagc ggcaaaaaat ttacaaggca tgaaaaaagc cttggacaag
     1141 gcagtaaccg ttgccactgc acagggctat gtcagtaaaa ccaaaatcaa agtcggtcaa
     1201 actgaattaa gggttactgc agcaactgac aaacaattgc tgaaaaccat tagcgaagga
     1261 agggacacga caggtaaaat gactgagcag ttatttgact ctttagctaa acaaaatggc
     1321 ttcaaagtgc tttcgggcgg caaatacggc gcaaataacg g
//
LOCUS       DAWFJW010000207         1325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWFJW010000207 DAWFJW010000000
VERSION     DAWFJW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.207"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>1325
                     /locus_tag="WGR19_002082"
     CDS             <1..>1325
                     /locus_tag="WGR19_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0799531.1"
                     /translation="YAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAAIVVIIV
                     TALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYSQAAVAI
                     INNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQ
                     TIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTT
                     FSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKH
                     KVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTA
                     LEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIG
                     TAGKAAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALS"
BASE COUNT          372 a          316 c          365 g          272 t
ORIGIN      
        1 tacgcctacc tgaaacagct tcagacggcc aagaacgtcg attggaaaca ggtgcagctg
       61 gcctacgaca aatgggacta taaacaggaa ggcttgaccg gagccggtgc agcgattgtg
      121 gtgattattg taaccgctct aacttatgga tacggagcgg ctgcagcggg tagcgtaact
      181 gccgcaggaa gtagtacagc cgcagctgca acaacgacag cggcagcaac taccactgtt
      241 tctactgcag ctgccatgca aaccgcagct ttagcctcct tgtatagcca agcagctgta
      301 gccatcatca ataataaagg cgatgtaggc aaagcattaa aagatctcgg caccagtgat
      361 acggtcaagc agattgttac ttccgcactg acggcgggtg cattaaacca gatgggagca
      421 gatattgccc aattgaacag caaggtaaga accgaactgt tcagcagtac gggcaatcaa
      481 accattgcca accttggagg cagattggcc accaacctca gcaatgcagg tatctcagct
      541 ggtatcaata ccgccgttaa cggcggcagc ttgaaagaca acttaggcaa tgccgcattg
      601 ggagcattgg ttaatagctt ccaaggggaa gccgccagca aaatcaaaac aaccttcagc
      661 gacgattatg ttgccaaaca gttcgcccac gctttggcgg gttgtgttag cggactggta
      721 cagggaaaat gtaaagatgg ggcaattggc gcagcagttg gggaaatcgt agctgaatcc
      781 atacttggcg gcagaaaccc tgctacactc agcgatgcag aaaagcataa agttatcagt
      841 tactcgaaga ttattgcggg cagcgtggcg gcactcaacg gcggcgatgt gaatactgcg
      901 gcgaatgcgg ctgaggtagc tgtggtgaat aatgctttga attttgacag cacccctacc
      961 aatgcgaaaa agcatcaacc gcagaagccc gacaaaaccg cactggaaaa aatcatccaa
     1021 agtattatgc ctgcacatgc ggcaggtgcg atgactaatc cgcaggataa ggatgctgcc
     1081 atttggataa gcaatatccg taatggcatc acaggcccga ttgtgattac cagctatgga
     1141 gtttatgcag caggttggac agctccgctg atcggtacag ctggtaaagc agctatcagc
     1201 acctgtatgg ctaatccttc tggttgtact gtcatggtta cgcaggctgc tgaagcgggc
     1261 gcgggaatcg ccacgggtgc ggtaacggta ggcaacgctt gggaagctcc agtaggagcg
     1321 ttgtc
//
LOCUS       DAWFJW010000208         1318 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWFJW010000208 DAWFJW010000000
VERSION     DAWFJW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.208"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(189..380)
                     /locus_tag="WGR19_002083"
     CDS             complement(189..380)
                     /locus_tag="WGR19_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799532.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(413..949)
                     /locus_tag="WGR19_002084"
     CDS             complement(413..949)
                     /locus_tag="WGR19_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799533.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLE
                     KDEIFKNVERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(1117..1317)
                     /locus_tag="WGR19_002085"
     CDS             complement(1117..1317)
                     /locus_tag="WGR19_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799534.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT          443 a          288 c          175 g          412 t
ORIGIN      
        1 tctttccaac tctgcacttt gatttttccg taaacaacag gattatcgtt aaacatcggt
       61 gcagcattca aacgataaga taaagaaacg acaacacacg ccaacagaaa aacatatttg
      121 aacttcatca tattttccac ataaagagca gcctgaaaat ctttcaggct gcccttgtca
      181 aattattcct agctttcggc ttttttggca aaccaaacaa tccgattacc cgcataatac
      241 tttccattta ttgaaatccg acaagccgcg cccaaaaaat gccatgcact gtcgatttcc
      301 gcagcaatct ttgtaccgtt ttcttcaaat tccaaatatt cacccaataa taaacttgaa
      361 acagaacgcg tgggcagcaa gtgcccaccc tacgcttatt caaataattt gattaaataa
      421 agaataaaga aaatgcatca ggaacaaaat tataatctgc cacctgactc acaccgcttt
      481 taaagtaagg ggcatcaaaa tcaaaaccgc aaaaaaaata atttttgcat tgatttttaa
      541 tagatttaaa attcgaatat agtgtttctc cagatttaaa aagtttatct ttatctatat
      601 atttgatgct ttccctatcc aaaataaata tttcaaacat taaaaaatca ttacatgacc
      661 aagccaaatc ataaatttga ttcaaatggg tatcataaag ataaaaataa taaggtttgg
      721 gaacaggtaa aatatttaat ggaaaaggag gactaatttt cttaatatct gatgactgat
      781 atccatatct ttctacattc ttaaaaattt catctttctc aagataacaa gacattctga
      841 caatcacaac ctgttcatcg gatatattat ctatttgcat ggcgcgtata acacgccatg
      901 cctgattaaa attagtctcc cttaccttag aatctattat cttttccaaa gatgaaaatg
      961 ccccttatct tgaactctcc agttagaacc tggaagtttc gtacctctta aatgtgtatt
     1021 aatatttctt atagtttcat taaaatgcca cgcgctgccg atttcaacgg taattttcgt
     1081 accgctttct tcaaattcca ggtattcccc cattagctaa cgcaaagaag cagacgccat
     1141 ttcggcttcg ttatgataaa cccgccttcc gttgatatag acttccgccc ctgtccgctc
     1201 caaattccaa aaattccgta ctgaaatttc catatcccga tattgtgcag accatgtttt
     1261 ttcgaaggtt ttcataaaat ttcctatacc tgtccaatcg gcacatatca attgcatt
//
LOCUS       DAWFJW010000209         1298 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWFJW010000209 DAWFJW010000000
VERSION     DAWFJW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.209"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            180..>1298
                     /locus_tag="WGR19_002086"
     CDS             180..>1298
                     /locus_tag="WGR19_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0799535.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGAQAQTQTHKYAIVMN
                     GQKLPEVKWGNDYSKLAQKSNEREVTYTSSFVRAKKNVSFLFNNTDNLIAEKKDAVVF
                     GAATYLPPYGKVSGFDTTKLTERKNAVDQIGTTHPGLIGYNYQNVTCSSGGCPELSYR
                     TQFTFGNSSLAKNKTDNKLDIYEDKSRDNSPIYKLKDHPWLGVSFSLGGESSFKPKRQ
                     GSLVSSFGEDVTQQNGTQDQYKSKNLVYTTDDYNSKGNKSHQDRHHAVAFYLNAKLHL
                     LDKKQIKNIAQGVTVDLGTLKTRIEPTQEWKNRSGNFFSNGNWTYEDKGSVSVKLKLP
                     QVKAGRCINKPNPNPNKKALSPALTAPALWFGPVQNGKV"
BASE COUNT          403 a          300 c          313 g          282 t
ORIGIN      
        1 gtcgggcatc tgcagcggtt tgctaaaaaa cgctttaccg tgatcagtgt gcaaagttaa
       61 aatggggagg taagcttttc aatcagcaat tcggcgggcg cggaatcggg cagtttaccg
      121 aaccccggcg ttcgcggcgc gccgccccgt cccgtgaagg caaactcaag gaataaaaga
      181 tgaataaaac ttggaaacgg caggttttcc gccataccgc gctttatacc gccatattga
      241 tgttttccca taccggcggg gggggggcgc aagcgcaaac gcaaacgcat aaatacgcta
      301 ttgtaatgaa cggacaaaag ctgcctgagg taaagtgggg gaatgactat agcaaattgg
      361 cgcaaaaaag caatgaacgc gaagttacct atacgagtag ttttgttaga gcaaaaaaga
      421 acgttagttt cttattcaat aataccgata atcttattgc tgaaaaaaaa gatgctgttg
      481 tttttggtgc agccacctac ctgccgccct acggcaaggt ttccggtttt gataccacta
      541 agctgaccga gcgcaaaaat gccgttgatc agattggtac gacccacccg gggttgatag
      601 gctacaacta ccaaaatgtc acttgctcca gcggaggctg tcctgaactt agctatagaa
      661 cccaatttac cttcggcaat tccagtttgg caaaaaataa aacagacaac aagctggata
      721 tatacgaaga caaaagccgc gacaattcgc ccatttacaa attgaaggat catccttggt
      781 tgggcgtgtc tttcagtttg ggcggagaga gctccttcaa accaaagaga caaggttctt
      841 tggtatcttc ttttggcgag gatgtgacgc agcaaaatgg tacacaagac caatacaaaa
      901 gcaaaaacct cgtttatacg acagacgatt acaatagtaa gggtaataaa agccatcagg
      961 acagacacca cgccgtcgcc ttttatctga acgccaagct gcacctgctg gataaaaaac
     1021 aaatcaaaaa tatcgcgcaa ggcgtaacag ttgatttggg taccttgaaa acgcgcatcg
     1081 agccgacgca agagtggaaa aaccggagcg ggaacttttt tagtaatggt aattggacgt
     1141 atgaagataa aggatcagtc agcgtcaaac tcaaattgcc gcaagtcaaa gcaggccgct
     1201 gcatcaataa accgaacccc aatcccaaca aaaaagccct ttcgccggca ctgactgccc
     1261 ccgcgctgtg gttcggacct gtgcaaaatg gcaaggtg
//
LOCUS       DAWFJW010000210         1284 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWFJW010000210 DAWFJW010000000
VERSION     DAWFJW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.210"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(228..1082)
                     /locus_tag="WGR19_002087"
     CDS             complement(228..1082)
                     /locus_tag="WGR19_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0799536.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          361 a          375 c          330 g          218 t
ORIGIN      
        1 cgtcattccc gcgcaggcgg aaatccggac atccaacgct gcagcaattt gtcggaaata
       61 accgaaactc aaaaaaccgg atgcccgcct gcgcgggaat gacgacgcaa agatggtcgt
      121 tatttcggat aaatatccgt cttttcgttc cggatacccg ctttcgcagg aatggagatg
      181 gaaagatttc agcctattcg gcaatcatca aataccgctt cccaacttta tttttgatgc
      241 gacagccggc cggcaaccaa ggtggcggcg atgacgacaa acacgcccaa ggcggacacg
      301 ggcgacaggt gttcgcccaa aatcaaaacc gccagcagca cgccgacgac gggttcgagc
      361 gaaatcaaca gtcccgaaac gttggcagga acacggctca tccccttgtt ccacagccaa
      421 taggcgtacc agccgcaccc caaacccaaa tacagcagcg acaataccat tccgacgctc
      481 cagtccacgg tataactttg cgccaaagca agcgaaaacg gcaggcacat caacgatgcg
      541 gcggcaatgg aaacagatgt gaatgccggt gcgccgatgc gtgcaatcag cctttgcgtc
      601 ggacgcatag cggcacaaaa gcccgcgccc gccaacaaca ccagcaggca gccgaaccag
      661 ccgacttcgc cgccctcttc cgcaccgccc gccatcagca gcgcgacccc ggcaaatgcc
      721 gccgcgccgc atatccagtg gtaggcacgc gctttgtcgt tgaagaaaaa gtgtccgaca
      781 aacaccatca gcagcggctc gagtccgaca atgaccgatg cgctggcggc ggaagtgtat
      841 ttcaacccga caaactgaag cagcagggtc agcacatagt tgacgaacga cacaatcagc
      901 aacggcttcc attcctcacg cggaatcttg ccgacatgac ggcggcaggc gggcagtgca
      961 ggcagcgcag caatcagcag gcgcacgccg accatcaatg cgggatcgat gccgccatag
     1021 acatatttgg cggcaataaa cgagctgctc cagataatca gggcaaggat ttggtaaaac
     1081 atagtgagtg ataaagtcag atggtcatac aaaagtatag cggattaaca aaaatcagga
     1141 caaggcgacg aagccgcaga cagtacaaat agtacggaac cgattcactt ggtgcttcag
     1201 taccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta
     1261 atccgctata acagcaacct tgtc
//
LOCUS       DAWFJW010000211         1267 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWFJW010000211 DAWFJW010000000
VERSION     DAWFJW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.211"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WGR19_002088"
     CDS             complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WGR19_002088"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0799537.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRHCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDTLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            723..1112
                     /locus_tag="WGR19_002089"
                     /pseudo
     CDS             723..1112
                     /locus_tag="WGR19_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
BASE COUNT          238 a          301 c          376 g          352 t
ORIGIN      
        1 ctggattccc gcctgcgcgg gaatgacggt ggagcggttt cagttgtttc taaataaatt
       61 tttgcagcgt tgggttttta gattcccgcc tgcgcgggaa tgacggtgga ggacgatgcc
      121 gtctgaatcc cattagcggt ttcagacggc attttcttgt cggtgcgaca gtcaggaaaa
      181 ctgcgccgcg ccacggtttg ccaaatcgtc ggcgcgttcg ttttcggcgt gtcccgcgtg
      241 tcctttaacc caagtccagc tgacttgatg ctgtccgacc agtgtgtcga gttctttcca
      301 caagtcgtcg tttttgacgg gctgtttggc ggcggttttc cagccgttgc gcttccaacc
      361 gtgtatccag ttttccatgc cgtttttaac gtattgcgag tcggtgcaga tgatgacggt
      421 gcagtggcgt ttgagggatt tcagtccttc gatgacggca gtcagttcca tgcggttgtt
      481 ggtggtttgc gcttcgccgc cgaaaagttc tttttcgtgg ctgccgtagc gcattaatac
      541 gccccagccg cccgcgccgg gattgccttt gcacgcgccg tcggtgtaaa ggtaaacggt
      601 ttggttcatt attgtgcctt tatgtggtgt ccggcgtgat tataagcggt ttgtgcgccg
      661 tgccgtctga aagattgcgg cggattaaaa taggcatata atgtgcaact ttatttttga
      721 atatgaagga gcggatagtg ggtcaaagcg gcgagttgtt ttgtttcgga caaatgcccg
      781 tatggaaggc ggaaaacctg ccggaaattt tgttgtcggg ttattcgtcc gaaggagggg
      841 aatgggtctg cctgaatgtg ttgcagggcg atgtcgaagt tcgtgcaccg gacgggtcgg
      901 cagaagtttg gtcggcagac ggcggcgatt gtgtgtttgc gccgcagcag gtgttttctg
      961 tcaaaccgaa aacggacgat gccgaaatcc gtttgtcgct gtattgcgcg gcggcagact
     1021 attttcacaa aaaatacggg atgagtgcca cgcattctgc ggtcgcggcg gcacaggata
     1081 ccgtaccggc gggcagggcg ttggatatgg gttgagacct ttgcaaaatt cctttccctc
     1141 ccgacagccg aaatccaaac acaggttttc ggctgttttc gccccaaata cctcctgatt
     1201 ttacccaaat acccccttaa tcttccctgg atacccgata atcaggcatc cgggctgctt
     1261 tttaggc
//
LOCUS       DAWFJW010000212         1231 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWFJW010000212 DAWFJW010000000
VERSION     DAWFJW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.212"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            50..322
                     /locus_tag="WGR19_002090"
     CDS             50..322
                     /locus_tag="WGR19_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799538.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPIGILPKPVALSIVQYED
                     DQGFYLFYLDETGQEQTDTYHDTLDSAFEQVEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="WGR19_002091"
     CDS             304..456
                     /locus_tag="WGR19_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799539.1"
                     /translation="MDAKPLGGNIKMPLYFSDGISVNKKVGIGAQNSLHLAGIGMKSL
                     RKNDYS"
     gene            443..988
                     /locus_tag="WGR19_002092"
     CDS             443..988
                     /locus_tag="WGR19_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0799540.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAEWVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            1091..1231
                     /locus_tag="WGR19_002093"
                     /pseudo
     CDS             1091..1231
                     /locus_tag="WGR19_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          392 a          214 c          256 g          369 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgcggtaac aattattcgg gattgaagaa tgatgagtgt
       61 aaaagaatta ttcaaagtca tcttagatga aaataaagat tttcctatta gaacgattca
      121 tcgcacgcca atagggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaga
      181 tgaccaagga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggtggagttt gaatttggaa ttagcaaaga
      301 agaatggatg caaagccctt aggtggcaat atcaagatgc cgctttattt ttcagacggc
      361 atttccgtca ataaaaaagt cggtattgga gcccaaaaca gcctacatct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtggctga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgacga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagccgaat gggtggaaaa
      901 aaagggttgg aatgcttttg agcagttctt gctggataat gaagttggca acctttcgga
      961 tatgaatcgg aaaccgttta cttggtaagg attttcttaa atttaatcaa agtgccgtct
     1021 gaaaccgtgt ttaattttca ggcgaccttc cccttatttt aaacaggata ttgggaacta
     1081 agttcttcaa aaatcctaca cctgctcctt ccacggcagc accttggtca aaacggcaga
     1141 cggctacaaa gccatcgcac atatccaaac tggagagcac gtctttgcca aagatgaaac
     1201 cagtggaaaa actggataca aacctgttac c
//
LOCUS       DAWFJW010000213         1212 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWFJW010000213 DAWFJW010000000
VERSION     DAWFJW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.213"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            213..1010
                     /gene="truA"
                     /locus_tag="WGR19_002094"
     CDS             213..1010
                     /gene="truA"
                     /locus_tag="WGR19_002094"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0799541.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALENALAQI
                     AGESVATTVAGRTDTGVHATAQVVHFDTVAVRPQQAWVRGVNAHLPEGIAVLHAQQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          294 a          386 c          278 g          254 t
ORIGIN      
        1 gtttctttag attttacgtc ctagattccc gcctgcgcgg gaatgacgat tcgggcactc
       61 ctgacagggt gaattcacag gatagcgatt cgtagcaact gcatccaccc ccccaactcc
      121 ccaaacaacg ccgctcgccc cgggcgtttg ccgtttccct gcaaaatctg cgatacaatg
      181 cagtctgaac atttatccga atcccaaatc cgatggatac cgcacaaaaa caacgctggg
      241 caataaccct atcctatgac ggcagccgct tttacggctg gcagaaacag gctgacggcg
      301 taccgaccgt tcaggcggca ttggaaaacg cacttgccca aatagcagga gaatctgtcg
      361 ccaccaccgt tgccggcaga accgataccg gcgtgcacgc caccgcccaa gtcgttcatt
      421 ttgatactgt tgccgtccgt ccccaacagg catgggtgcg cggcgtaaat gcccacctgc
      481 ccgaaggcat tgccgttttg cacgcccaac aggtcgcccc cgaatttcat gcacgatttg
      541 acgcatacgg acggcactac cgctacctgc tcgaatccgc ccccgtccgt tccccactgc
      601 tcaaaaacag ggcaggctgg acacacctca aactcgacat cgggccgatg cgccgggctg
      661 ccgcctattt gattggcgaa caagacttct ccagcttccg cgccgccgag tgccaagcaa
      721 aatcccccgt caaaaccatc taccgcgccg atcttaccca aagcgcagga ctcgtccgcc
      781 tcgatttgca cggcaacgcc tttttgcacc atatggtacg caacattatg ggcgcgctcg
      841 tctatgtcgg cagcggcagg ctcagcgtcg aaggcttcgc cgccctcatt caagaacgca
      901 gccgcctcaa agccccaccg acctttatgc ccgacgggct ttacctgacc ggcgtcgact
      961 atcccgaggc atacggcatc atccgccccc aaatccccga atggctttaa aacattcttg
     1021 tcgcggagat tttgaaatcg gacaaactgt caggcaatct ttttcaatgt tgacactacc
     1081 tcatcaaggt aataacattg ttattacata aactggtaaa tagggtacgt atatgattct
     1141 caacatacga aaaatgggaa actcgcaagg cgtgattctg cccaaatcat tattgggtca
     1201 aataggggca gt
//
LOCUS       DAWFJW010000214         1206 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWFJW010000214 DAWFJW010000000
VERSION     DAWFJW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.214"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            156..485
                     /locus_tag="WGR19_002095"
     CDS             156..485
                     /locus_tag="WGR19_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0799542.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            514..762
                     /locus_tag="WGR19_002096"
     CDS             514..762
                     /locus_tag="WGR19_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0799543.1"
                     /translation="MASSGVNNEIKDKKLSLWAKRQDGSVKWFCGQPVTRADTDTDTA
                     VTAASDKQIDPKHLPSTCRDESTAGCIETPPTAFYKNT"
     gene            857..>1206
                     /locus_tag="WGR19_002097"
     CDS             857..>1206
                     /locus_tag="WGR19_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0799544.1"
                     /translation="MRGNDEAILLAEGQKSAVTEYYLNHGEWPANNSSAGVASSATDI
                     KGKYVEKVEVKNGVVTAEMASSNVNKEIQGRKLSLWAKRQAGSVKWFCGQPVTRADKA
                     TDADADVTADSGNE"
BASE COUNT          357 a          341 c          302 g          206 t
ORIGIN      
        1 gaaaaagcat ggaaccttta cgaaaaaaat aaaacagtcc aacaaacaca gccgtttcag
       61 ctgcaccgat gcagctgacg tttgagcggc atttgacttc gacggacggt atgttaccct
      121 tgccgttttg accaacaagc aacgaaagga caaatatggg cggcctgatt attgaagact
      181 tgcaagaagg cttcggaaaa gaagcagtta aaggcaaaga gattaccgtg cattacacag
      241 gttggctgga aaacggaacc aaattcgact ccagcctcga ccgccgccag ccgctgacca
      301 tcacgctcgg tgtcggacaa gtcatcaaag gctgggacga aggcttcggc ggcatgaagg
      361 aaggcggcaa acgcaagctg accatccctt cggaaatggg ctacggcgca cacggcgcgg
      421 gcggcgtgat tcccccgcac gccactttga tatttgaagt cgagctgctg aaagtgtacg
      481 aataaagctg cccgggcaat accgtctgac ataatggctt caagcggcgt aaacaacgaa
      541 atcaaagaca aaaaactctc cctgtgggcc aagcgtcaag acggttcggt aaaatggttc
      601 tgcggacagc cggttacgcg cgccgacacc gacaccgaca ccgccgttac cgccgccagc
      661 gacaagcaga tcgaccccaa gcacctgccg tcaacctgcc gcgatgaatc aactgccggt
      721 tgcatagaaa caccacctac ggctttctat aaaaatacct aaaccgtcat tcccacgaaa
      781 gtgggaatcc agaacgcaaa atctaaagaa accgctttcc cgataagttt ccgtaccgac
      841 agacctagat tcccgcctgc gcgggaatga cgaagccatt cttttggctg aaggtcaaaa
      901 atcagccgtc accgagtatt acctgaatca cggcgaatgg cccgccaaca acagttctgc
      961 cggcgtggca tcctccgcca ccgacattaa aggcaaatat gttgagaaag ttgaagtcaa
     1021 aaacggcgtc gttaccgccg aaatggcttc aagcaacgta aacaaagaaa tccaaggcag
     1081 aaaactctcc ctgtgggcca agcgtcaagc cggttcggta aaatggttct gcggacagcc
     1141 ggttacgcgc gccgacaaag ccaccgacgc cgacgccgac gtcaccgccg acagcggcaa
     1201 cgaaaa
//
LOCUS       DAWFJW010000215         1192 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWFJW010000215 DAWFJW010000000
VERSION     DAWFJW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.215"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            1..1192
                     /locus_tag="WGR19_002098"
                     /pseudo
     CDS             1..1192
                     /locus_tag="WGR19_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
BASE COUNT          350 a          222 c          324 g          296 t
ORIGIN      
        1 gcagccgtgg ccgatgctgc ctttaccgca ttggtgagcc aggcttccat ttccctgatc
       61 aacaacaaag gcaatatcgg taacaccctg aaagagctgg gcagaagcag cacggtgaaa
      121 aatctggtgg ttgccgccgc taccgcaggc gtagccgaca aaatcggcgc ttcggcactg
      181 aacaatatca gcgataagca gtgggtcaat gacctaaccg tcaacctggc caatgcgggc
      241 agtgccgcgc tgattaatac cactgttaac ggcggcagcc tgaaagacaa tctggaagcg
      301 aatatccttg ctgctttggt gagtactgcg catggagagg cagccagtaa aatcaaacag
      361 ttggatcagc actacattac ccacaagatt gcccatgcca tagcgggctg tgcggcagcg
      421 gcggcgaata agggcaagtg tcaagatggt gcgatcggtg cggcggtcgg tgaaatcctt
      481 ggcgaaaccc tactggacgg cagagaccct ggcagcctga atgtgaagga cagggcaaaa
      541 atcattgcta aggcgaagct ggcagcaggg gcggttgcgg cgttgagtaa gggggatgtg
      601 agtacggcgg cgaatgcggc tgctgtggcg gtagagaata attctttaaa tgatatacag
      661 gatcgtttgt tgagtggaaa ttatgcttta tgtatgagtg caggaggagc agaaagcttt
      721 tgtgagtctt atcgaccact gggcttgcca cactttgtaa gtgtttcagg agaaatgaaa
      781 ttacctaata aattcgggaa tcgtatggtt aatggaaaat taattattaa cactagaaat
      841 ggcaatgtat atttctctgt aggtaaaata tggagtactg taaaatcaac aaaatcaaat
      901 ataagtgggg tatctgtcgg ttgggtttta aatgtttccc ctaatgatta tttaaaagaa
      961 gcatctatga atgatttcag aaatagtaat caaaataaag cctatgcaga aatgatttcc
     1021 tagactttgg taggtgagag tgttggtggt agtctttgtc tgacaagagc ctgcttttcg
     1081 gtaagttcaa caatatctaa atctaaatct ccttttaaag attcaaaaat tattggggaa
     1141 atcggtttgg gaagtggtgt tgctgcagga gtagaaaaaa caatatacat ag
//
LOCUS       DAWFJW010000216         1189 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWFJW010000216 DAWFJW010000000
VERSION     DAWFJW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.216"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..429
                     /locus_tag="WGR19_002099"
     CDS             <1..429
                     /locus_tag="WGR19_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799545.1"
                     /translation="AIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQ
                     PVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTV
                     DVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            436..945
                     /locus_tag="WGR19_002100"
     CDS             436..945
                     /locus_tag="WGR19_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799546.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          405 a          216 c          205 g          363 t
ORIGIN      
        1 gctataccaa cccagacagt taaagaactt gatggcttac tacaagaatc aaaaaatata
       61 ggtgctgtaa atacacgaat taatatagcg aatagtacta ctcgatatac accaatgaga
      121 caaacgggac aaccggtatc tgctggcttt gagcatgttc ttgaggggca cttccatagg
      181 cctattgcga ataaccgttc agtttttacc atctccccaa atgaattgaa ggttatactt
      241 caaagtaata aagtagtttc ttctcccgta tcgatgactc ctgatggcca atatatgcgg
      301 actgtcgatg taggaaaagt tattggtact acttctatta aagaaggtgg acaacccaca
      361 actacaatta aagtatttac agataagtca ggaaatttga ttactacata cccagtaaaa
      421 ggaaactaac taaatatgag taactttgaa aaaaaatata ttttagaatt aaatgatgct
      481 ttaagccatt taaatcataa ctctacctca tttgatttat tgaaagtttt gatttcatgg
      541 ttatcaaacg atattgtcat tgataaattt aaaattttag gttatgactt tagtaaatat
      601 atcgaaatga atcccgatga ctatccggtt gaaaaatcta tattgaatag agaggaaatt
      661 atttatctca aaaacaatat ttatcgtaaa atatcatcag gaaattttaa atttcaatac
      721 tttgtacaat atattagaga tattttagaa tatttattta ttgaacatat tgaaagagtc
      781 tgtccttact gcgaatgggg tgaaatgcaa aaattagaag aacaaaatac gcatgaaacg
      841 gtgtatctct gtactcaatg tggatgtgct ttttataacg ataattcaca atttttatta
      901 aaaacccctt taaccattcc aatgaaacgt gatgaattta aataaacaag ccgtagcctg
      961 catgaaccct aaaatccacg tgtagcgtgt gtgcgccagc acgcatgcgt tccatgattt
     1021 acggctcaat gccgtctgaa aagctcacaa tttttcagac ggcatttgtt atgcaagtaa
     1081 atattcagat tccctgtatg ctgtacagac gcgggagtgt taagcccccc ttgtttgaag
     1141 ctccgcggct cctgccgagc ttcaccgacc ccgttgtgcc caagctctc
//
LOCUS       DAWFJW010000217         1167 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWFJW010000217 DAWFJW010000000
VERSION     DAWFJW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.217"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(216..707)
                     /locus_tag="WGR19_002101"
     CDS             complement(216..707)
                     /locus_tag="WGR19_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ0799547.1"
                     /translation="MIFKQDQNYWAVFYANEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTLDNEAERIDFLKQENFKPRYEDWVAKLCGNLGYKTKRA
                     LFKNMMSVDIWLHNGCLKISPSRHVKLEAWDAIDADDVILSLDNSPEEIGAGLKLALS
                     RCR"
BASE COUNT          309 a          268 c          231 g          359 t
ORIGIN      
        1 cattgaatat actgaaagat gtcatccaat aattcagatt taaatttctc aatcttttcc
       61 atatttgcca aattccttaa taactttatc ttcagcaagc cgtaacccac attaaatctg
      121 caggtaaggt gtggtacgca cgccagtgaa cttgagtcta ataaaaaggt cgtctgaaac
      181 atttttccgg ctttcagacg gccttttgcc aaatattatc ggcagcggct caatgccaac
      241 tttaaacctg ctccgatttc ttcagggctg ttatccaatg ataaaattac atcgtctgca
      301 tcaatggcat cccacgcttc cagcttgaca tggcggctcg ggctgatttt caggcagccg
      361 ttgtgcagcc aaatatccac gctcatcatg tttttaaata gggcgcgttt ggttttatag
      421 cccaagttcc cgcatagctt ggcaacccaa tcctcatagc gtggtttaaa attttcctgt
      481 tttaaaaaat caatacgttc agcttcgttg tctaacgtcc tgctgtttgc caacgcttgc
      541 aagaccgttg tgcctaaagt ttcattgtcg gtatccaatg gcaggatatg ggggggatat
      601 aggtggtcta ttgccgttaa ccccaaacct gaacatgttt gaacaatcag agcttcttca
      661 ttcgcataaa aaactgccca ataattttga tcctgtttaa aaatcattat ttgatctccg
      721 taattttgac tttaatgttt caatttttgc catactctac cacacgttgc aactgcaatc
      781 tttgctcttt atatattgca ccccatcaaa gggctgcatt acttttcttt ctaaagatta
      841 cttttgtata aaaagtattt ttcttaagcg atagggatta cgtccgcgcc atattcagac
      901 atcgcccgga tgttgcgctg cccgatcgga tgcttcagac ggcatcggaa gggtttgcgg
      961 ttttggaata tgaagatgat aatgtccgcg agattgacgg catttgaaag cggttatcct
     1021 acataccgga ggacacgaaa tgaacgaata ttcccaatta atcaagcatc ccgatgtttc
     1081 cctttccccg atttcagacg gcatcggggt cgacaatccg gcaacgggcg aaattttggc
     1141 gtatgtccgc aagacggatt cggacaa
//
LOCUS       DAWFJW010000218         1127 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWFJW010000218 DAWFJW010000000
VERSION     DAWFJW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.218"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..437)
                     /locus_tag="WGR19_002102"
     CDS             complement(<1..437)
                     /locus_tag="WGR19_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799548.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKL"
     gene            complement(582..1013)
                     /locus_tag="WGR19_002103"
     CDS             complement(582..1013)
                     /locus_tag="WGR19_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799549.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
BASE COUNT          300 a          191 c          233 g          403 t
ORIGIN      
        1 agtttgtcgt agttgagctc ttctacggca tagagtgtca atttccgccc ggcttcgatg
       61 ttaatgctgc ctttgggggc atcgaatgcg gtggcgtagg cgtcgatgct gccaccagat
      121 ttgatgtcga tgccttggga tgcgctgagg tttactgcgt cgggcttggc gtttttgtgt
      181 tctttgactt cggtaatgtg tttgcggttg taccatttgc cggttttgta gctgcggcgt
      241 tcaaaggtat agagttcgct ctgtctcgca tagtaatatt ggtcaccgta agattgaatt
      301 ttgattttgc cgttttccga actgatatcc gtggtgctga gcaggatgcg actgttctca
      361 ttggcatacg gtgcgctaat gctcacaccg gttttacccg aaagttctgc agcaagcgta
      421 gggggtgtct tcagcatgca cgcgtctagg cagtatatag ggaatctgaa tatttacttg
      481 cataacaaat gccgtctgaa aaattgtgag cttttcagac ggcattgagc cgtaaatcat
      541 ggaacgcgtg cgtgctgaag cacacacctt acgcatggat tttaggtttc atgcaggcta
      601 cagtttgttt gagaatatgt tttttgtatt tctttacact ttttttgata ttcagggtga
      661 gctattaaga aatcagatat tgcaatatta aagtaatgat atgctaaatt aataggtata
      721 attaattctt caggattgaa atcatctcca actgaaaaat aaatagaagg agctttatca
      781 atatactcaa atccatttac ctcttcataa tctccaatcc atctagtagc atctattttt
      841 tcatattgag cacctattac tccaaaatgt ccggtagttg taatattttt aaaacactct
      901 aaaagcgtat ctttagtttc aaattggtaa attgtttcaa aaaaaatcga aataacaaaa
      961 tggttgttat ttatagattt tttatttaag tttagaaaaa tattattttt cataattttt
     1021 cctttaaatt attctgggtg aatgtttgta attcttcctg tatttacatc aaaatatgat
     1081 ctaaatttga ttccgtcaaa ggtttctgaa aattgaatga cattttt
//
LOCUS       DAWFJW010000219         1102 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWFJW010000219 DAWFJW010000000
VERSION     DAWFJW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.219"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            800..>1102
                     /locus_tag="WGR19_002104"
     CDS             800..>1102
                     /locus_tag="WGR19_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799550.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDY"
BASE COUNT          275 a          285 c          226 g          316 t
ORIGIN      
        1 aaggtcgtct gacaattctc aaaacggctg aggttgtgaa tttgggcaac caatactttc
       61 tttgccaaaa aaatattttc tttcaaactc aacttgtatt tgtcgggaaa cacgataatt
      121 ccttaaataa ttaaagcagt acgttaaatc atcatcttat catcactttc ggggtttatg
      181 ttgcatgcgt ccgttttcta atacggtttc ccatcccgcg aaacgaggcg gagacctgtg
      241 caaaaaacct gttcacgaaa ttttccgcat taacgatacg ctgaaaatgc gctaaaaatg
      301 actgtgtttg aatatcggtt gatttcatcc gtttgtgtaa cctcgcgccg gttttgtcat
      361 gattttgcat tatagtgaat taaatttaaa ccagtacagc gttgcctcgc cttgccgtac
      421 tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaattc actatatttt
      481 tgtcatgcgg atatttcacg ggatgacaaa acgggcgcaa aaaagcccga ttggaaaatc
      541 cgaatcgggc ttttttgtga tgttctgttc gatttgcgta ctaactgccg gccggagcgt
      601 ccgcgcccgg tttggcaggt cggattctcg aatccgacgg ctatttgaga tggcaggctt
      661 ttgtcggata caagtatccg acctacggct tgcttttcgt cattcccgcg aaagcgggaa
      721 tctagaatct cggactgaga cctttgcaaa attccccaaa atcccctaaa ttcccaccaa
      781 gacatttagg ggatttctca tgagcacctt cttccaacaa accgcccaag ccatgattgc
      841 caaacacatc gaccgtttcc cgctattgaa attggaccgg gtgattgatt ggcagccgat
      901 cgaacaatac ctgaatcgtc aaaaaacccg ttacctccga gaccaccgcg gccgtcctgc
      961 ctatccactg ttgtccatgt tcaaagccgt cctgctcgga caatggcaca gcctctccga
     1021 tcccgaactc gaacacagcc tcatcacccg catcgacttc aacctgtttt gccgtttcga
     1081 cgaactgagc atccctgatt ac
//
LOCUS       DAWFJW010000220         1093 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWFJW010000220 DAWFJW010000000
VERSION     DAWFJW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.220"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            284..721
                     /locus_tag="WGR19_002105"
     CDS             284..721
                     /locus_tag="WGR19_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HEZ0799551.1"
                     /translation="MSRYLITFDMDTNCLKNNFHGNSYNNAYADIKHILARHGFEKIQ
                     RSVYLGREGISEAHGTIAIQELTARFDWFSSCISNIKFYRIESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
BASE COUNT          339 a          245 c          225 g          284 t
ORIGIN      
        1 gccaccaaaa cccttgctag ctaagggtta aacagctcac ttgaaatcta cttaagtcta
       61 atctaaacta tccaatatgg atagattttt aaacataggg caagcagcaa aattattgta
      121 gctgaaagca caatcactcg ctggtggtct caaacacgtg ccgactacct cgccgaaaac
      181 actatcagct gcgataaacc gtgggaaaag ctcggtatca tgcgccgcac ttggtattat
      241 cgcgggaaac cgatgccgtc tgaaacgcaa caggagaaaa ataatgagcc gttacctgat
      301 tacctttgac atggatacca actgtctgaa aaacaatttc cacggaaata gctataacaa
      361 tgcctatgcg gatattaaac acatcttggc tagacatgga tttgaaaaga ttcagcgcag
      421 tgtttatcta ggccgtgaag gcatcagtga agcacacgga acaatagcca ttcaggaact
      481 gaccgctcgg tttgattggt tttcctcctg tatttcaaac attaagtttt accgcattga
      541 aagcgatttg aacgcacaat ttatcgctga tggtgtgtat caagccaaac aggctttcct
      601 tcaacgtgtt gaacaacttc gtatatccct aacagaagct ggattgtctg atgagcaaat
      661 caatcaggtt ctggaaaaac agaaatttga attggaaagt cctaacctga aattaaatta
      721 acctccttta ctctccaaca tccgccgcag ttctgtcagt ttttggcgcg ctgcggcgtt
      781 ttctgtgcgt tttagagctt cgggtagggt gtgaaacagc tcactcgaaa tctacttaag
      841 tctaatctaa actatccaag cagtaattag tacaaaaaaa gcaaacttat tttaggagct
      901 tatgatgact gaaaaattgc agccgcgata aaccgtggga gagtctcggc atttcccacg
      961 ccacttggta caaacgtggc aaaccgatgc cgtctgaaac cgtacaacag gagacaaaat
     1021 aatgcttgct caaattgaac tcacaccctg ccagctaatg gtttccgtaa cttaaggtta
     1081 ctgggtttgc gca
//
LOCUS       DAWFJW010000221         1091 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWFJW010000221 DAWFJW010000000
VERSION     DAWFJW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.221"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..197)
                     /locus_tag="WGR19_002106"
     CDS             complement(<1..197)
                     /locus_tag="WGR19_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799552.1"
                     /translation="MNLVKLLANNWQPIAIIALIGTGLAVSHHQGYYKSAFAKQQAVI
                     DKMEKDKAQALLLSAQNYAREL"
     gene            complement(169..405)
                     /locus_tag="WGR19_002107"
     CDS             complement(169..405)
                     /locus_tag="WGR19_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799553.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            complement(408..572)
                     /locus_tag="WGR19_002108"
     CDS             complement(408..572)
                     /locus_tag="WGR19_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799554.1"
                     /translation="MGQVEFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(575..748)
                     /locus_tag="WGR19_002109"
     CDS             complement(575..748)
                     /locus_tag="WGR19_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799555.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAARKRKNRR"
     gene            complement(745..>1091)
                     /locus_tag="WGR19_002110"
     CDS             complement(745..>1091)
                     /locus_tag="WGR19_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0799556.1"
                     /translation="RGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQVLSKAVAKK
                     TGWKLRGEDGFKPDNAGRHSRLAYAQAGGIVFEPFFISNDTDLALFKTTKWGICRAIA
                     DAIAMELGAAKV"
BASE COUNT          246 a          334 c          248 g          263 t
ORIGIN      
        1 agctcgcggg cgtagttttg agccgacaac agcagggctt gcgctttgtc tttttccatc
       61 ttgtcaatga ccgcctgctg cttcgcaaac gccgacttat aatagccttg atggtgcgac
      121 accgccaaac ccgtgccgat aagtgcgatg atggcaattg gttgccagtt attcgccagc
      181 agtttcacga gattcattct cgacctcctg acgtttgacg ctgacaaacg agcgcgccac
      241 cgcatagccg ccgacaatgc ccaaatacac cgcccaaatc tccgccgacg gatcgggcag
      301 catcacaaac ttaaacgtcc ccgccgcgca ggcaacgttt gcccacagtt tcgagtgcga
      361 cacattgcct gtcgcggggt ttttaaaaat atccaaaata cgcattgcta ttccacactt
      421 ttggtttgca ggtgccgttt cagcatttcc tgataattgg ccagttcgcc ctccgcaaat
      481 tcaaacgccg ccaagtctgc ctgttcactt gcctcacggc ttttggccga ccacagccca
      541 atcatctttt cgtaaaactc aacctgtccc atgattaacg acgattcttg cgtttacgcg
      601 ccgcgcgttt cgcagccgct acgcccgact tgcccaggcg catagacgga tgttgtttca
      661 aatagccaat actggcaggc ttaatctcaa attcaggcat ctgcggtttc aagacagaca
      721 gagccaaagc aatcaaagac tttttcatac cttcgccgct cccaattcca tcgcaatcgc
      781 gtccgcaatc gcgcggcaga tgccccattt ggtcgtctta aacaaggcca aatcagtgtc
      841 gttgctgata aaaaacggct caaacacaat gccgccggct tgcgcataag ccaggcgcga
      901 atgccgaccc gcattgtcgg gtttaaagcc gtcttcgccg cgcagtttcc agccggtttt
      961 cttggcaacg gctttgctca acacctgaca ccagcgtttg tttttaacgg tactcaaggc
     1021 ttcgatgcct gtcgccgctt tgctgacggc agcgttggtg tgaaactcaa tcgccacatc
     1081 cgagccgcga a
//
LOCUS       DAWFJW010000222         1073 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWFJW010000222 DAWFJW010000000
VERSION     DAWFJW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.222"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(122..550)
                     /locus_tag="WGR19_002111"
     CDS             complement(122..550)
                     /locus_tag="WGR19_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0799557.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WGR19_002112"
     CDS             complement(531..965)
                     /locus_tag="WGR19_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0799558.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          281 a          322 c          194 g          276 t
ORIGIN      
        1 ttgaaaaccc cattaaaccg tctttaaccc atcgtcctga acggtagatg tttcaccccc
       61 tgcgaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 ccttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt accgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaacgccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca acattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttcg
      841 gaaacatcca tatccgcaca cgaccgcttg cccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcgt ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcatctcaa acccataaaa cacaatatat tgactaatta acgcatatta taccaataaa
     1021 atacaatata tagtattaag ccgatgtttt ttgcgaaacg gacagacata aaa
//
LOCUS       DAWFJW010000223         1001 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWFJW010000223 DAWFJW010000000
VERSION     DAWFJW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.223"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            19..>1001
                     /locus_tag="WGR19_002113"
     CDS             19..>1001
                     /locus_tag="WGR19_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0799559.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYTQFDVGNRGAILNNSRSNTQTQLGGWIQGNLWLARGEA
                     RVVVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTTGQPQY
                     QAGALTGFQVRSGHIAITGQGLDARDTDFTQILSHAVKIDGPVWGKDVRVSAGKNNVS
                     ADGSIRSSHSPATNINSDNSLYAIDTGALGGMYAGKITLISTDRDASVRNQG"
BASE COUNT          259 a          221 c          250 g          271 t
ORIGIN      
        1 aatcaaataa ctgaaatcat gaataaaact ctctatcgtg taattttcaa ccgcaaacgt
       61 ggggctgtgg tagccgttgc tgaaactacc aagcgcgaag gtaaaagctg tgccgatagt
      121 gattcaggca gcgctcatgt gaaatctgtt ccttttggta ctactcatgc acctgtttgt
      181 cgttcaaata tcttttcttt ttctttattg ggcttttctt tatgtttggc tgtaggtacg
      241 gccaatattg cttttgctga tggcattatt gctgataaag ctgctcctaa aacccaacaa
      301 gccacgattc tgcaaacagg taacggcata ccgcaagtca atattcaaac ccctacttcg
      361 gcaggggttt ctgttaatca atatacacag tttgatgtgg gtaatcgcgg ggcgatttta
      421 aacaacagcc gcagcaacac ccaaacacag ctaggcggtt ggattcaagg caatctttgg
      481 ttggcaaggg gcgaagcacg tgtggttgta aaccaaatca acagcagcca ttcttcacaa
      541 atgaatggct atattgaggt gggcggacga cgtgcagaag tcgttattgc caatccggca
      601 gggattgcag tcaatggtgg tggttttatc aatgcttccc gtgccacttt gacgacaggc
      661 caaccgcaat atcaagcagg cgcattaaca ggctttcagg ttcgttcggg tcatattgcg
      721 atcacgggac aaggtttgga tgcacgtgat accgatttca cgcagatatt gagccatgcg
      781 gttaagattg atgggcctgt gtggggtaaa gatgtgcgtg tgtcggcagg gaaaaataat
      841 gtctcagctg atggctctat ccggtcttca cactcccctg ctacgaatat aaattccgac
      901 aactcactgt atgccatcga tacaggggca ttgggcggta tgtatgccgg taagatcacc
      961 ctgatcagca ctgatcgtga tgcatccgtg cgcaatcagg g
//
LOCUS       DAWFJW010000224          995 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWFJW010000224 DAWFJW010000000
VERSION     DAWFJW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.224"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(52..438)
                     /locus_tag="WGR19_002114"
     CDS             complement(52..438)
                     /locus_tag="WGR19_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799560.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPISKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            complement(441..686)
                     /locus_tag="WGR19_002115"
     CDS             complement(441..686)
                     /locus_tag="WGR19_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0799561.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMDKGK"
     gene            complement(764..>995)
                     /locus_tag="WGR19_002116"
     CDS             complement(764..>995)
                     /locus_tag="WGR19_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0799562.1"
                     /translation="KLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDGIIYG
                     YTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
BASE COUNT          303 a          191 c          143 g          358 t
ORIGIN      
        1 aaaaatgccg tctgagcggc ggaaatggct ttcagacggc attttgactg tttaaagcgg
       61 gggcagttct acaaacggaa agaaatgctg aaacctctga taaatttcag gatgtttctt
      121 taaggctttt aatgcttttt cttttgaaat gggaggatcg tagacatcta tcccccttaa
      181 aaaagcaata ccggttaatg cattatcctg attttttgat aagttatttt cgggaacaaa
      241 tcctataata tcaatcagat cgtatagtga ttgaaaatct atcaaatcaa aaaaataatt
      301 tggaatttca tctaaatcca cttcccttat gattttttcg acccacattt taaattcggt
      361 aagattgatt gcttggcaat atatactgcc tatggcaaaa tttaagtcgg tgctattttc
      421 tttataaatt ttccacataa tcatttacct ttatccattg ccgtccctcc tatgtgaccg
      481 gttttagaat gttcccattc aggtacaagc tgcatacgtc cagtatcctg atgatgatgc
      541 caagtataat ccggtatttt agattctcct ttttcgattg cctttagttg ctttgttgta
      601 aaagcagaac tacgtacttc tcctcgttta atggcttccg ccaaatcctt ggttgccagc
      661 ctcatttgcc ctgtagaatc cttcaatctg aactccttat gcttacataa acataattac
      721 cagtttcagt atctaaaaaa cttgcttaaa ttacttaaat tagctaataa aaatcatcgc
      781 gagagaattt ttttccagtc ggtataatat atttcttatc tatatatata caatatttta
      841 tatcaaaaag taaaatagta tagccataga ttataccatc ttcttcgctt atacctaata
      901 ccactccttt ttttccttta atagtctcac cattttctaa caaatcatta gggcaagaat
      961 cttttataat aacttcagag tagaaatcta atttt
//
LOCUS       DAWFJW010000225          983 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWFJW010000225 DAWFJW010000000
VERSION     DAWFJW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.225"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            30..983
                     /locus_tag="WGR19_002117"
                     /pseudo
     CDS             30..983
                     /locus_tag="WGR19_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
BASE COUNT          227 a          293 c          255 g          208 t
ORIGIN      
        1 tttttgttaa tccactatat caagtctgcg tgttggaaaa acagcacttt ccgcgcttcg
       61 tcatcggcga aagcctgctg ccgcactgta tggaaatgct ggaagaagcc ggatttgccg
      121 atgccgtgcg cgccgagccc ggctttcagt tgaaaaacgg cgcggcgttt tcgtggggca
      181 gccgttatac cgaattcgat ttcaccgata aattttcaga cggccccggc acgacttatc
      241 aagtgcgccg cgccgtgttc gataaaatcc tgattgaaga agccgccaaa caaggggcgt
      301 tgaagtacgt ttcgggcacg gcgtaaccgc gttcgacaac agcggcgatt ttgcccgcct
      361 gaacatcgaa accgacaccg gcaagcgcta tgaactgacc gcgaaattcg tcttggacgc
      421 aagcggttac ggacgcgtgc tgccgcgcct gctaaacttg gaaacgccct cgcacctgcc
      481 gccgcgccaa gcgcatttca cgcacatcga cgacaacatc accaacccga aattcgaccg
      541 caacaaaatc ctgattacca cctatccgca acaccgcgac gtgtggattt ggctgattcc
      601 cttcggcgac aaccgctgtt ccgccggcgt ggtcggcacg cccgacaaac ttgtcggcga
      661 atcggaaacg gtgttgaaaa aatttgttta cgaatgcccg atgctgaacg aaattttgga
      721 caaagccgtt tgggaaaacg attttccgtt ccgctccatc caaggctatt ccgccaatgt
      781 gaaatcgctg cacggcaggc atttcgcgct gttgggcaat gccgccgagt tcctcgatcc
      841 cgtgttctcg tcgggcgtaa ccatcgcgct gcactccgcc aaacttgccg ccgatctgtt
      901 ggcaaaacaa ctcaaaggcg aagccgccga ttggcaaacc gaatttgccg aacccttgat
      961 gatcggcgtg gacgcgttcc gca
//
LOCUS       DAWFJW010000226          979 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWFJW010000226 DAWFJW010000000
VERSION     DAWFJW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.226"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>979
                     /locus_tag="WGR19_002118"
     CDS             <1..>979
                     /locus_tag="WGR19_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0799563.1"
                     /translation="NNVSDKQWINNLTVNLANAGNAALINTAVNGGSLKDNLEANILA
                     ALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGE
                     ALVKNTDFSRMSATEIEKAKAKITAYSKLVAGTASAVVGGDVNTAANAAQIAVENNTL
                     YPRCVGAKCDEFQKEQQKWIRENPEEYREVLLFQTGFIPIIGDIQSFVQAQTAADHLF
                     ALLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAVTVATAQGYVSKTKIKVGQTELR
                     VTAATDKQLLKAIGEGRDTTGKMTEQLFDSVAKQNGFRVLSGGKYGANNG"
BASE COUNT          299 a          194 c          267 g          219 t
ORIGIN      
        1 tgaacaatgt cagcgataag cagtggatca acaacctgac cgtcaacctg gccaatgcgg
       61 gcaatgccgc actaattaat accgctgtca acggcggcag cctgaaagac aatctggaag
      121 cgaatatcct tgcggctttg gtgaatactg cgcatggaga ggcagccagt aaaatcaaac
      181 agttggatca gcactacatt gcccataaga ttgcccatgc catagcgggc tgtgcggcag
      241 cggcggcgaa taagggcaag tgtcaggatg gtgcgatagg cgctgcagtc ggtgagattg
      301 ttggtgaggc tttggttaag aatactgatt tcagtcgtat gagtgcgacc gaaatcgaaa
      361 aagctaaagc gaagattact gcctattcaa aactggttgc cggcactgcg tctgccgttg
      421 taggcgggga tgtgaataca gcggcgaatg cggcacagat agcggtggag aataatactt
      481 tgtatcctag atgcgttggt gcaaagtgtg atgaatttca aaaggaacaa caaaaatgga
      541 tacgtgaaaa tcctgaagaa tatcgagaag ttttgctttt tcagacagga tttattccaa
      601 ttatcggtga tatacagagt tttgtacaag cacagaccgc tgccgatcac ctgtttgctt
      661 tgctgggtgt ggttccgggt atcggtgaat cgatacaggc ctataaagta gcgaaagcgg
      721 caaaaaattt acaaggcatg aaaaaagcct tggacaaggc agtaaccgtt gccactgcac
      781 agggctatgt cagtaaaacc aaaatcaaag tcggtcaaac tgaattaagg gttactgcag
      841 caactgacaa acaattgctg aaagctattg gcgaaggaag ggacacgaca ggtaaaatga
      901 ccgagcagtt atttgactct gtagctaaac aaaatggctt cagagtgctt tcgggcggca
      961 aatacggcgc aaataacgg
//
LOCUS       DAWFJW010000227          946 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWFJW010000227 DAWFJW010000000
VERSION     DAWFJW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.227"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>946
                     /locus_tag="WGR19_002119"
     CDS             <1..>946
                     /locus_tag="WGR19_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0799564.1"
                     /translation="DKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAA
                     ETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNA
                     LVSLSDKAKAAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKN
                     IYQNLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLA
                     YGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVG
                     FGHNKNVSLYGNDGNDTLIGGAGNDYLEGGSGSDTYVFG"
BASE COUNT          283 a          205 c          224 g          234 t
ORIGIN      
        1 agacaaagtg gaactcactg ccgaacaggc aaaagccgcc aatcttgcgg gcattggtcg
       61 tctgcgcgat ttgcgcgaag ctgccgcatt gtccggcgat ttggccaata tgctgaaagc
      121 ttattctgcc gccgaaacta aagaagcaca gttggcattg ttagataatt tgattcacaa
      181 atgggcggaa accgattcga actggggcaa aaaatcgcca atgcgacttt caaccgattg
      241 gacgcaaacg gctaatgaag gtattgcact gacaccatcc caagtagcac aactaaaaaa
      301 gaacgcttta gtttcccttt ctgataaagc taaagcagct attgacgccg cccgcgaccg
      361 cattgccgtg cttgatgcct acacggggca ggattccagc acgctttatt acatgagcga
      421 agaagacgcg cttaatatcg tcaaagtaac caacgataca tacgaccatc tcgccaaaaa
      481 catctaccaa aacctgttgt tccaaacccg tttgcagcca tatttgaatc aaatcagttt
      541 caaaatggaa aatgatacgt tcactttgga ttttagtggt cttgttcaag catttaacca
      601 tgtcaaagaa actaatccgc aaaaagcttt tgtggatttg gccgagatgc ttgcatatgg
      661 cgaacttcgt tcttggtatg aaggccgaag actaatggcc gattatgtgg aggaggcaaa
      721 aaaagcaggt aaatttgaag attaccagaa agtgttgggt caggagaccg ttgcattatt
      781 agctaaaaca tcgggtacgc aagcagatga tatcctgcaa aatgtaggct ttggtcataa
      841 taaaaatgtt tctttatatg gtaatgacgg caacgacact ctgatcggcg gtgccggtaa
      901 tgattacttg gagggcggca gcggttcgga tacttatgtc ttcggc
//
LOCUS       DAWFJW010000228          915 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWFJW010000228 DAWFJW010000000
VERSION     DAWFJW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.228"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            32..>915
                     /gene="tuf"
                     /locus_tag="WGR19_002120"
     CDS             32..>915
                     /gene="tuf"
                     /locus_tag="WGR19_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0799565.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVL"
BASE COUNT          248 a          257 c          228 g          182 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtat tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgatga
      481 ctgcccgatt gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttatgaaga
      541 gaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcgaaga cgtgttctcc atttccggcc gaggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggcgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caagtattgg ctaaaccggg taccatcact cctcacacca agttcaaagc
      901 agaagtatac gtact
//
LOCUS       DAWFJW010000229          897 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWFJW010000229 DAWFJW010000000
VERSION     DAWFJW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.229"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..215)
                     /locus_tag="WGR19_002121"
     CDS             complement(<1..215)
                     /locus_tag="WGR19_002121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799566.1"
                     /translation="MPSVELLLVIVGLPRGTFYYQLVVQSAEDKYADLKRHIHDIYQK
                     QLKDNGLVQSMSRKGNCLDNAAMESFFG"
     gene            complement(247..663)
                     /locus_tag="WGR19_002122"
                     /pseudo
     CDS             complement(247..663)
                     /locus_tag="WGR19_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          209 a          215 c          197 g          276 t
ORIGIN      
        1 ccgaagaaac tttccattgc cgcattgtcc aagcagtttc ccttgcggga catactctga
       61 accagaccgt tgtctttcaa ctgcttttga taaatatcat ggatatgccg tttcaaatcg
      121 gcatatttgt cttctgccga ttggacaacc aattggtaat agaaggtgcc gcgtggcagt
      181 ccgacaatca ccaacagcag ttcaacggat ggcattgcct taaccctgcg acgagttgcg
      241 ttctttctac cgcacttctt tcccatagat taaggcatcg agctttttta gggcagccat
      301 ttccgcttta aggcaagcca attccgcaag cagttcttcc ttggttttca gatagtcggc
      361 tttttcgttt ccagcggatg ctgttttttc acgggctttc ttcctttggg tttagggttt
      421 gggctttaaa ccgttaatac cattcaaatg gtagaggcgc aaccattgca gcaagatgga
      481 gcagtcgggc aaattcagtt ggtctgcggc agctttttgg gacattccct accccgtcac
      541 caggcggatt gccccaagtt tgtattcgac cgaatatttt gtcgtatgct ttctgcgttt
      601 gatgccactc tctccgtgta atctgtattt tgtcacccat ctgcgtacca atgaatcgga
      661 aatagaaaga tggtctgctg ttccctgcca aatagtattg aacgacggca agtcggaatt
      721 catctgaata ttttgccata aaaaactgca ccccctaaag tcggtaaggt gtccaacttt
      781 tggggtgcag ttcagaagcg gtcttttttt gcctgccggt tttgaatcat cctccgtgta
      841 tattcccttg acgaaaaaaa tgatgatatt acggatacca aaactaaggt cgtatcc
//
LOCUS       DAWFJW010000230          874 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWFJW010000230 DAWFJW010000000
VERSION     DAWFJW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.230"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(55..834)
                     /locus_tag="WGR19_002123"
     CDS             complement(55..834)
                     /locus_tag="WGR19_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0799567.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          203 a          289 c          173 g          209 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgg gacacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg ccgaaccctt ccga
//
LOCUS       DAWFJW010000231          866 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWFJW010000231 DAWFJW010000000
VERSION     DAWFJW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.231"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(1..734)
                     /gene="mafB"
                     /locus_tag="WGR19_002124"
                     /pseudo
     CDS             complement(1..734)
                     /gene="mafB"
                     /locus_tag="WGR19_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
BASE COUNT          210 a          213 c          194 g          249 t
ORIGIN      
        1 cctactcctt gaaaacccat taaaaaagga ttaaccgcac cttggacgat gccgcgaata
       61 tcatccatac ggttagccca ccaattttta ttggggtcgc tttcccatat cagttttcct
      121 gcttcatccg cacggctgaa aaaaccagag gcggcaccgg cattttcttt tagccaacgg
      181 tctgaaaatg gggcttgagg gacaatatta gtctttgttt ttgttgaagt tcatttgaca
      241 taatagctgt atatatccct tgctcctccg gggggcggat aatcgctgcc ttgcggtccg
      301 tcatatccat cctccggatg aatttccgac cctgtccgat gaagttggta aacagtaaaa
      361 ccgccgtcta caccgccgct gaaatcagaa gtgctttttg aatcatgatg atcgaacgga
      421 ctgtgtactt catgtccgtg ccctgaaaaa tgggtttcat aaccgataac accttcaaac
      481 cctgtccgtt cgtgtgtaat aggcagtatg gggccgaccg cagttgcatc aaatgtttgg
      541 acggcgtaaa cccgattttt aacactgccg cgaccatcac cgaacagatg gtatttacca
      601 cccggttcgt agtgttttgc ctgcatatca tcgcgcaaac gggcatccaa accgttcgca
      661 tgactaatgg ggatttgcag caacgatatt gctgctgcaa acagcatcat gaatttttga
      721 ataggcaaat tcattttgtt ttccttggta gcaagcggtt atggtagtgt gaatcaaggt
      781 tgcccttgtc tatgttgtcg cactacttca tcgctgtatc catacccctc atgactgtta
      841 tcagtctcta cggatggggc ggagtt
//
LOCUS       DAWFJW010000232          830 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWFJW010000232 DAWFJW010000000
VERSION     DAWFJW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.232"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          212 a          158 c          234 g          226 t
ORIGIN      
        1 gaagagaaga gaagagaaga gaagagaaga gaaggttttt tggggctgga ttcattttcg
       61 actccgtatt cggtttaact gattaaaaag aacgattttc actgatggtt caagggcgga
      121 ttatatcggg tttggggcga tgtttcaaca caatatagcg gatgaacaaa aaagagaacg
      181 attctctaag atactgaagc accaagtgaa tcggttccgt actatctgta ctgtctgcag
      241 ctcgtcgcct tgtcctgatt tttgttaatc cgctataaag accgtcgggc atctgcagcc
      301 gtcattcccg cgcaggcggg aatctagaat ttcaatgcct caagaattta tcggaaaaaa
      361 ccaaaaccct tccgccgtca ttcccacgaa agtgggaatc tagaaatgaa aagcagcagg
      421 aatttatcgg aaacgaccga aaccgaacgg actggattcc cgcttttgcg ggaatgacgg
      481 gattttaggt ttctgatttt ggttttctgt ccttgtggga atgacgggat gtaggttcgt
      541 aggaatgacg tggtgcaggt ttccgtgcgg atggattcgt cattcccgcg caggcgggaa
      601 tctagaccgg tcggtgcgga aacttatcgg gaaaaaaggt ttcttgagat tttacgtcct
      661 ggattcccac tttcgtggga atgacgggat tttaggtttc tgattttggt tttctgtcct
      721 tgtgggaatg acgggatgta ggttcgtagg aatgacgtgg tgcaggtttc cgtacggatg
      781 gattcgtcat tcccgcgcag gcgggaatct agaccttaag gcagcggcaa
//
LOCUS       DAWFJW010000233          814 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWFJW010000233 DAWFJW010000000
VERSION     DAWFJW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.233"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            144..530
                     /locus_tag="WGR19_002125"
     CDS             144..530
                     /locus_tag="WGR19_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0799568.1"
                     /translation="MKGLDKYILFKNCETLGELFRIYQDYLSLNIDDPNLKIKFFDLL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRKIWPDMEEMLLYFPDNPWHYVEWF
                     WWGATCPIELTQLPKIEIYEEQMKQA"
     gene            564..814
                     /locus_tag="WGR19_002126"
                     /pseudo
     CDS             564..814
                     /locus_tag="WGR19_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580210.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
BASE COUNT          291 a          148 c          173 g          202 t
ORIGIN      
        1 ccttggaaag gaacgaccat cattttaagg aatttttcaa ctagccgaga acaaactgga
       61 gcaaaatgga cgattgagtt tcaaaatcat ccattagtag gtaggaaaaa aatggaatta
      121 aaatttagat aaggaattga ataatgaaag gtttagataa gtatatccta tttaaaaatt
      181 gtgaaacatt aggagaattg tttcgaatat atcaagacta tctatcatta aatattgatg
      241 acccaaattt aaaaattaaa ttttttgacc ttttggaaga acttttatca gatggtgtta
      301 ttgaattatg cgattatcgt gaaaatcctc caaaaatatt aaccggttcg ccaaaaacac
      361 aggtagatga attaagaaaa atttggccgg atatggaaga gatgttgcta tattttccag
      421 ataacccttg gcattatgtt gaatggtttt ggtggggagc aacttgccct attgaactga
      481 cacaattgcc taaaatagaa atttacgaag aacaaatgaa acaagcttaa agccagcgta
      541 accagcctgt atgacaaggt gcaggtgaac aatccaatat ccacattacc ggctcagatg
      601 ttgccggcaa agaaggaacc ggcctgattg cagataagca aatccatctg caatcagccg
      661 agcaaagtaa taccgaacgc agccaaaaca aatcggcagg ctggaacgca ggtgctgccg
      721 tatcattcgg acaaggaggc tggtcattag gcgttaccgc aggcggcaat atcggcaaag
      781 gccacggcaa tggcgacagc atcacccacc gcca
//
LOCUS       DAWFJW010000234          805 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWFJW010000234 DAWFJW010000000
VERSION     DAWFJW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.234"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..743)
                     /locus_tag="WGR19_002127"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="WGR19_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0799569.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAWFJW010000235          783 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWFJW010000235 DAWFJW010000000
VERSION     DAWFJW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.235"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(510..668)
                     /locus_tag="WGR19_002128"
                     /pseudo
     CDS             complement(510..668)
                     /locus_tag="WGR19_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          251 a          147 c          155 g          230 t
ORIGIN      
        1 ttgctggaag aaggtgctca tgagaaatct cctaaatgtc ttggtgggaa tttaggggat
       61 tttggggaat tttgcaaagg tctcaaacgc tgaatctgtg aatcttgaca gctttgccga
      121 ccggctggaa cgcatcggcg atgtggattt ttcgagtttg ttcggtcact aaaattttag
      181 aggctgtact agataaccag accaaattcc cattaactaa ttgtcttcaa atctgaattt
      241 tagattctat ttcactgttg ttaaaacgct attcacattc ctttaaatac cgtccaaaat
      301 gctctttggg aatgctattg gatcatagac cttacaacag aaaaaaggtc ggaaagtaga
      361 aagactgctt tttaaattca aacggccaag caagtaaaga cttggccaac aaatgggaca
      421 aaatccgact gttttaaatt gccagtcaaa tactggaaat gggacaactt ccgattgttt
      481 taaattgcca gtcaaatact ggaaaacttg cgtaaatggc taaattcact gacatcaagt
      541 acatcataac tatgaaaggt atccgtatac acaatgccat cagacttaac tttctctcgg
      601 ataatcggca ataatgttgc tgattgcgca ttagggacaa cgacggtata aaccttgcca
      661 tttcgttcga atgggatttt taaccgttat ctagtaaagt ccctaattta ataaacaaat
      721 ggcagtaaaa tggcggtaat tgactcgcca aaatccttga tctagaatgg tcagactatc
      781 caa
//
LOCUS       DAWFJW010000236          770 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWFJW010000236 DAWFJW010000000
VERSION     DAWFJW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.236"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            152..721
                     /locus_tag="WGR19_002129"
     CDS             152..721
                     /locus_tag="WGR19_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0799570.1"
                     /translation="MLFPRIIAASAQRKLSVYLKKGGLVAYPTESCYGLGCLPTLAKA
                     LGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPAPKTFLLSAKSC
                     VLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRACRTEREVRRQFG
                     RDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT          209 a          151 c          214 g          196 t
ORIGIN      
        1 aaggcagccc ggatgcctga ttatcgggta tccgggcagg attaagggga tatttgggta
       61 aaattagtgg atatttgaaa cgaaaacagc cgaaaacctg tgtttgggtt tcggctgtcg
      121 ggagggaaag gaattttgca aaggtctcat tatgttattt ccgagaatta tcgcagcatc
      181 agcgcaacgt aagttaagcg tttatttgaa aaaaggcggt ttggtcgctt atccgaccga
      241 atcttgttat ggcttggggt gtcttccgac attagccaaa gcactcggta agttggcgca
      301 tttgaaaaaa agaccgcaac acaagggtat gattgtcatc gggaatcaat ttgaacaatt
      361 gcagccttta cttcaaatgc cgtctgaaaa tcttcaggat atgcttagaa aagaatggcc
      421 tgcccctaaa acgttcctat tatccgccaa atcctgtgtc ttacctgagt tgcgcgggaa
      481 acagcgcagc aagttggcgg tcagggttcc cgcccatgta ggtgcgcgcc gcttgtgtca
      541 ggctttgcaa acgcctttgg tctcgacttc gtgcaaccgc gcaggcaaac gcgcgtgtcg
      601 gacggagagg gaagtcagaa ggcaattcgg acgcgatgtg tggattgttg gtgggagaat
      661 cggcaggcag aaatcgccga gtcagattat tgacggggaa acaggtaaac gtttgcgtta
      721 gatatgggta cttgaatgaa aacttttttg catacacaaa gaaaaaattt
//
LOCUS       DAWFJW010000237          764 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWFJW010000237 DAWFJW010000000
VERSION     DAWFJW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.237"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            9..>764
                     /locus_tag="WGR19_002130"
     CDS             9..>764
                     /locus_tag="WGR19_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0799571.1"
                     /translation="MKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADK
                     RLAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQA
                     DLALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPI
                     TNVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTD
                     IEGITRIKYEIPTLDRTGKPDGGFKEISSIKTVY"
BASE COUNT          282 a          136 c          147 g          199 t
ORIGIN      
        1 aggtagcggt gaaaaataat cagcttagcg acaaagaggg tagagaattt gataacgaaa
       61 tgactgcatg cgccaaacag aataatcctc aactgtgcag aaaaaatact gtaaaaaagt
      121 atcaaaatgt tgctgataaa agacttgctg cttcgattgc aatatgtacg gatatatccc
      181 gtagtactga atgtagaaca atcagaaaac aacatttgat cgatagtaga agccttcatt
      241 catcttggga agcaggtcta attggtaaag atgatgaatg gtataaatta ttcagcaaat
      301 cttacaccca agcagatttg gctttacagt cttatcattt gaatactgct gctaaatctt
      361 ggcttcaatc gggcaataca aagcctttat ccgaatggat gtccgaccaa ggttatacac
      421 ttatttcagg agttaatcct agattcattc caataccaag agggtttgta aaacaaaata
      481 cacctattac taatgtcaaa tacccggaag gcatcagttt cgatacaaac ctaaaaagac
      541 atctggcaaa tgctgatggt tttagtcaaa aacagggcat taaaggagcc cataaccgca
      601 ccaattttat ggcagaacta aattcacgag gaggacgcgt aaaatctgaa acccaaactg
      661 atattgaagg cattacccga attaaatatg agattcctac actagacagg acaggtaaac
      721 ctgatggtgg atttaaggaa atttcaagta taaaaactgt ttat
//
LOCUS       DAWFJW010000238          756 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWFJW010000238 DAWFJW010000000
VERSION     DAWFJW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.238"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(152..>756)
                     /locus_tag="WGR19_002131"
     CDS             complement(152..>756)
                     /locus_tag="WGR19_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799572.1"
                     /translation="DLLNGDAGNDSIYSGNGNDTLNGGEGNDALYGYNGNDVLNGGEG
                     NDHLNGEDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDTVYNYHVDKNSDTMHFKG
                     FKAADVHFIRSGSDLVLSASEQDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYIN
                     AGNNLVQSMSVFGSNTAATGGNVDANTQSVQQPLLVTPSA"
BASE COUNT          215 a          181 c          141 g          219 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatattgc tagaagtgca ccgattaagg ttaattaaaa
       61 tacgaccctt atcccaatat taagaaaaac agcaatcaaa ataaacttga ttgctgtttt
      121 tcagtttaag ccatgaatgt gattaggctc cttatgcaga tggcgttacc aataacggct
      181 gctgtacgga ttgtgtattg gcatccacat ttcctcctgt cgcagcagta ttagaaccga
      241 acacagacat agactgtacc aaattattgc cagcattaat atacttggca aaatctggat
      301 tactgatagc tgcatcatca aagacaaatg tatctacacg atggttttca ccatagaaga
      361 atccggaaat acgtacgttg tcttgttcag aagcgctaag caccaaatca cttccggaac
      421 ggataaaatg aacatctgct gctttaaatc ctttaaagtg catagtgtca gagtttttat
      481 ccacatggta attatagacc gtatcctgac cgaagccttt gccgaagaca taagtatccg
      541 aaccgctgcc gccctccaag taatcattgc ctgcaccgcc gatcagagtg tcgttaccgt
      601 cttcgccgtt caaatgatca ttgccttcgc caccattcag tacatcgtta ccattatagc
      661 cgtacagggc gtcgttgcct tctcctccat tgagcgtatc attgccattg ccactgtaga
      721 tactgtcgtt gcctgcatca ccattcagca ggtcat
//
LOCUS       DAWFJW010000239          700 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWFJW010000239 DAWFJW010000000
VERSION     DAWFJW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.239"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            223..700
                     /locus_tag="WGR19_002132"
                     /pseudo
     CDS             223..700
                     /locus_tag="WGR19_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          211 a          181 c          159 g          149 t
ORIGIN      
        1 gcgggaatga cggttcgggc attccttaaa tcacccgtgt atcgctgtaa atcttagaga
       61 tggcggaata tagcggatta acaaaaacca gtacggcgtt gtcgcgcctt agctcaaaga
      121 gaacgattct ctaaggtact gaagcaccaa gtgaatcggt tccgtactat ttgtactgtc
      181 tgcggcttcg tcgccttgtc ctgatttttg ttaatccaat atactttatg ccgctaccgc
      241 aaccgactgg ttcaagacaa taccctgtct gaactgttgg aactgattaa ctgccaactg
      301 accgaaaaag gtttaaaaat agagaaagca tccgccgccg tcattgacac caccattatt
      361 cagaccgccg gcagcacaca gcgtcaggct atagaagtcg atgaagaagg acaaatcaac
      421 ggccaaacca caccgagtaa ggacagcgat gcccgttgga tcaagaaaaa cggcctctac
      481 aaactcggtt acaaacaaca tacccgtacc gatgcggaat gctatatcga gaaactgcac
      541 attacccccg ccaatgccca tgagtgcaaa cacctgtcgc ctttgctgga aggactgccc
      601 aaaggtacga ccgtctatgc cgataaaggc tatgacagtg cggaaaaccg gcaacatctg
      661 aaagaacatc agttgctgga cggcattatg cgcaaagcca
//
LOCUS       DAWFJW010000240          686 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWFJW010000240 DAWFJW010000000
VERSION     DAWFJW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.240"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..485)
                     /locus_tag="WGR19_002133"
     CDS             complement(<1..485)
                     /locus_tag="WGR19_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0799573.1"
                     /translation="MTKLYAEIAKREAQDDGTVKVLGYASSEAVDSDGEVIATAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDNLNKSQITGLKLTEISLVERPANPDAVFTCFKADKGAEAVNNDTEHNAT
                     Y"
BASE COUNT          154 a          188 c          154 g          190 t
ORIGIN      
        1 aaatatgtag cattatgttc tgtatcgttg tttaccgctt ccgcaccttt gtccgcctta
       61 aagcaggtaa acaccgcatc gggattggca gggcgctcga caaggctgat ttccgtcagc
      121 ttcaagcccg tgatttgcga cttgttcaaa ttatcgcggg cggtaacgct gccgccgatg
      181 gaaaagcctt tgtaaacgcc tgttttgact ttcgacacga caacggggtc gatgatatga
      241 gcaccgaaaa aggttatgcc gtcgtcttca acgttgattt caattgccgt tcccgcagcg
      301 tttgagccgt gcatctcgcg ccccgcgtca aacttcatat aatcgggaat cgccgccttc
      361 atagctgccg tcgcgatgac ttcaccgtcc gaatcgaccg cctcgcttga ggcataaccc
      421 aaaaccttga ccgtgccgtc gtcctgcgcc tccctcttgg cgatttctgc gtataacttt
      481 gtcatgggtt tactcctgcc tgtataaatg gcatgctgaa tttaaagccc ttttgtaagg
      541 ctggaagtgt acttgcctta acgctatccg ctccagaacg acactgcaaa agggctttac
      601 tcatccttca agatgaaatg gtcgtaatgt ttatggccgt ctggaaattc ccttacaatc
      661 acaccgatat ttaaagactc attccc
//
LOCUS       DAWFJW010000241          686 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWFJW010000241 DAWFJW010000000
VERSION     DAWFJW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.241"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          199 a          137 c          166 g          184 t
ORIGIN      
        1 gattagagtt tcaaaattta ttctaaatag ctgaagctca atgaaccgga ttcccgcctg
       61 cgcgggaatg acgaatttta ggttgctgtt tttggttttc tgtttttgtg aaaataacgg
      121 gatttcagct tgtgggtatt tactggaaaa aacagaaacc gctccgccgt cattcccgcg
      181 caggcgggaa tccagacatt caatgctaag gcaatttatc gggaatgact gaaactcaaa
      241 aagctggatt cccactttcg tgggaatgac gaagtggaag ttacccgaaa cttaaaacaa
      301 gcgaaaccga acgaaccgga ttcccacttt cgtgggaatg acgggatgca ggtttccgta
      361 tggatggatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcagtgc ggaaacttat
      421 caggtaaaac ggtttcttga gattttgcgt cctggattcc cactttcgtg ggaatgacgc
      481 gattagagtt tcaaaattta ttctaaatag ctgaaactca atgaaccgga ttcccgcctg
      541 cgcgggaatg acgaagtgga agttacccga aacttaaaac aagcgaaacc gaacgagccg
      601 gattcccgct tgcgcgggaa tgacgggatt aagttttcaa aattcatcag aaattactga
      661 tttaatagca taaatttttt agatta
//
LOCUS       DAWFJW010000242          676 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWFJW010000242 DAWFJW010000000
VERSION     DAWFJW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.242"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          201 a          173 c          117 g          185 t
ORIGIN      
        1 aaaacagaaa tcccaaaccc ccgtcattcc cgcgaaagcg ggaatccagg ttcgttgagt
       61 ttcggtcatt tccgataaat tcttgcaact ttgcgtttct agattcccac tttcgtggga
      121 atgacggtaa agattgctgt tttcccgaca aattcccgcc atctaaaatc tcgttatcca
      181 tacaagaacc gaaaatcttg ccattctcac aaaaacagaa attcaaaaac agaaatccca
      241 aaccctcggc attcccgtga aagcgggaat ccaggttcgt tgagtttcgg tcatttccga
      301 taaattcttg cagctttgcg tttctagatt cccactttcg tgggaatgac ggtaaagatt
      361 gctgttttcc tgacaaattc ccgccatcta aaatctcgtt atccatacaa gaaccgaaaa
      421 tcttgccatt ctcacaaaaa cagaaattca aaaacagaaa tcccaaaccc tcgtcattcc
      481 cgcgaaagcg ggaatccagg ttcgttgagt ttcggtcatt tccgataaat tcttgcaact
      541 ttgagtttct agattcccac tttcgtggga atgacggcgg agggttgctg ttttcccgac
      601 aaattcccgc catctaaaat ctcgttatcc atacaagaac cgaaaatctt gccattctca
      661 caaaaacaga aattca
//
LOCUS       DAWFJW010000243          660 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWFJW010000243 DAWFJW010000000
VERSION     DAWFJW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.243"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(64..624)
                     /locus_tag="WGR19_002134"
     CDS             complement(64..624)
                     /locus_tag="WGR19_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0799574.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPA
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT          180 a          152 c          130 g          198 t
ORIGIN      
        1 gctaaatatc tagcaaaaaa gtgcggtcaa aatttaccgc acttttcgtt tcgaacaatc
       61 gaattattta atcacgccac atgccatacg tgggccgcca ccgccaagtg gagctggatg
      121 atcggagtga ttatcaccac ccgtgtggat cataatagag tgaccgcgaa catcatctaa
      181 atgtttaaga cgtggtgcta aaacaggatt tgttgctgtg ccatcatgca atacagttaa
      241 tgcaggtaaa tcacctaagt gtgcatcatc ttgccatggg taaccatgtt gttttgcacc
      301 tttaggatcc cagtgaccgc ctgcgcctaa accagctgtc aatttacctt ctttttcttt
      361 tggctcacag cttgggtttt catggatgtg gaaaccatgt aagccttcgc ttaatccttg
      421 taaatcaggg gtaaacacaa gaccatagtt agattcagta atagtcactg tacccacatc
      481 tttgttaccg tttgctggat caagttgttg cactttcact tcaatagaag cacctttagg
      541 tatcgtatta tgctcgtgtg cttgcgcaac accaactgaa catactgcac taaccgctag
      601 tgctaataag gttttcatat tcattgcaat ctcctttatt gcagtaaaaa ctcgcgacta
//
LOCUS       DAWFJW010000244          659 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWFJW010000244 DAWFJW010000000
VERSION     DAWFJW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.244"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(58..543)
                     /gene="fxsA"
                     /locus_tag="WGR19_002135"
     CDS             complement(58..543)
                     /gene="fxsA"
                     /locus_tag="WGR19_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0799575.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          193 a          209 c          122 g          135 t
ORIGIN      
        1 tatattttca aaccggaaaa atgccgtctg aaccttcaga cggcattcca ttcacattta
       61 ttcgtctttt ttgtgttcga tggcgtttcg ggaacgattg ccgccgtaag gctcttcaac
      121 cgtatattcc ccctcgataa tatcgtcatc gcgggaaaag ccctcttttc tgcccgattg
      181 gttcatgttg aaaaaatttt ccgcacctcc tgcctgcaac actgcacctc ccttaaacgg
      241 cagcagcagc aataccgcca acaccgagga tacgaatccc ggactcatca gacacaccgc
      301 cgccaccgta taacggatag gccacaacat ctgataaacg gataccctcc cgccgcttct
      361 cattgccgcg cccgccaata aaagaccgga cagccccgta tgcctgagca tcagcacgcc
      421 ggcggcaaag gttgccgcca ttagaaacag cgtccaaccg ccgcccaacc aatcggcaac
      481 ccacacaatc gacataatct ccaaaaacag cagcaccaaa aaaccgatac cgaaaaatct
      541 cattgaccgt catccttata tttaagtaaa cagcaaaccg cccgaacagg actccaagcg
      601 agctgcctgt aaatgattac aaaaccatgt gcttcaagcc gaaacaatgt gaaatcacg
//
LOCUS       DAWFJW010000245          654 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWFJW010000245 DAWFJW010000000
VERSION     DAWFJW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.245"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..180
                     /locus_tag="WGR19_002136"
     CDS             <1..180
                     /locus_tag="WGR19_002136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799576.1"
                     /translation="LAVVTAAPLALAAHANATLPDTAKNALEAAKADGMEAGWIVVGV
                     FAALFVFSIVKRVMK"
     gene            187..465
                     /locus_tag="WGR19_002137"
     CDS             187..465
                     /locus_tag="WGR19_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799577.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          178 a          137 c          165 g          174 t
ORIGIN      
        1 ctggctgttg taacagctgc tcccctggct ttggccgcac atgcaaatgc aacgttgccc
       61 gatacagcaa aaaacgcttt ggaagccgca aaagcggacg gtatggaagc cggttggatt
      121 gtcgttggcg ttttcgccgc gctttttgta ttttccatcg ttaagagagt gatgaagtaa
      181 gacggcatgt actaccaagt cggaaataaa tgtcttgaga agcaccaggc tgaaaacctt
      241 tatttcagct tggtagtacc aagaatcaaa gaaaacggac agattgtcag gccggaatat
      301 aacggcagcc tgtggaagat gtcggacggt cagccgctaa ggcttttatt ggcggaatgc
      361 agtctgaaag acaacctgca aagcggcctt gaaacaggct ggatagtatt cggcatcctc
      421 gcgtccgttt actttgtttc cctgctgaaa aaggttttga aatgatggat ttttattttt
      481 atctcggcgt ttccgtaccc gtattaatcg gggcggttct gtttaagaat tgagcgcatg
      541 aagttatggt gtcaaaatca ggctttcaaa acaatcattg aaaggcagaa ccatgaacaa
      601 gccgtttatc actcaggcgc agttggcact ttataaatat cagccgtcta gcaa
//
LOCUS       DAWFJW010000246          621 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWFJW010000246 DAWFJW010000000
VERSION     DAWFJW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.246"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          168 a          126 c          164 g          163 t
ORIGIN      
        1 ctttcggtgc ggaaacttat cggataaaac ggttttccct gcatcctgga ttcccactgt
       61 cgtgcgaatg acgggatata ggtttcacta cggacgtgtt cagattaccg cctgcgcggg
      121 aaggacggga tttcaggttg ctgtttttga ttttctgttt ttgtgggaat aacgggactt
      181 tggatgatgt gcatttatcg ggaaaacaaa aaaccctccg ccgtcattcc cgcgaaagcg
      241 ggaatctaga attttaatgc tgcaagaatt tatcggggat gaccgaggtt tgaaggtctg
      301 gattcccgcc tgcgcgggaa tgacggttta gaaatcaccc gaaacgagaa aaagaaccga
      361 aaccgaacaa gccggattcc cacctgcgcg ggaatgacgg gatttcaggt tactgttttt
      421 gattttctgt tttgtgggaa taacgggatt ttggatgatg tgcattttcg ggaaaactaa
      481 aaaccctccg ccgtcattcc cgcgaaagtg ggaatctaga attttaatgc ggcaagaatt
      541 tatcggaaaa aactgaagtt taaagaccta gattcccgcc tgcgcgggaa tgacggttta
      601 gaaatcaccc gaaacgagaa a
//
LOCUS       DAWFJW010000247          615 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWFJW010000247 DAWFJW010000000
VERSION     DAWFJW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.247"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(3..398)
                     /locus_tag="WGR19_002138"
     CDS             complement(3..398)
                     /locus_tag="WGR19_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799578.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFVKVSG"
     gene            complement(382..>615)
                     /locus_tag="WGR19_002139"
     CDS             complement(382..>615)
                     /locus_tag="WGR19_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0799579.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          171 a          123 c          114 g          207 t
ORIGIN      
        1 acttagcctg agacctttac aaatttgtcg gtttcggtgt cgtattggta gcctcgtgcc
       61 tgtatgtctt ctttgaaagt ttcgtatacg tcgtgggcta aaagggctgt tccgacatag
      121 ggaaccgccc ttgtgctgaa tttcgcgcct aagcgggcaa gtttgccgcc ccccgccaat
      181 acgccggcgc gggaaatgct ggcggttatt ttggcgttga ttcgggcttt tgcgcccgta
      241 gggatgtgtg ttaaatctac cgtttttatt aaatcagatg aataagtttt actattttta
      301 ggtacaaact tatgaatttt cgcaccttgt ccggtatcaa ctgaaacagt ttcagatatt
      361 tttactgcat ttgcattcgc ttcaaacgaa tacatcatca aaattgcaat tatcgacaat
      421 ttcgcaaaat tcaaatttgt atattttatg accatctttc agagattctt taattaccat
      481 ttctgaatta tcagaaaatg agattagcca aatatcatgt ttaattcttc tattccagaa
      541 aaaagagaaa caatcaataa cattttcaga cttattaatc ttcgcaaatt caacaaattc
      601 agattgcgct ataac
//
LOCUS       DAWFJW010000248          611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWFJW010000248 DAWFJW010000000
VERSION     DAWFJW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.248"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..393
                     /locus_tag="WGR19_002140"
     CDS             <1..393
                     /locus_tag="WGR19_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0799580.1"
                     /translation="QKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMAAKETLSY
                     FDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLSAAEYQDIFDFMKQYDLS
                     YPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT          176 a          136 c          137 g          162 t
ORIGIN      
        1 caaaagcggt tgtacgatta ccgcttcttc ctcgctttcg ccgaacagga tgccgaggcg
       61 atgaaagccg ccttagagcc gcttttcgat aaaaaaaccg cgcgtatggc tgccaaagaa
      121 acattgtcct atttcgattt ctacctgcag ccgcaaatcg ttacctatgc caaaatcgca
      181 tccatgcacg gtttcgattt gggcatagat caagaaatcg caccgaggga tttgattgtt
      241 tacgatccgc tgtcggcagc cgaatatcaa gacatcttcg attttatgaa acagtatgac
      301 ttgtcttacc cgtatgaata tctgcaggat tggatagatt actatacgtt caaaaccgat
      361 aagctggtat ttggtaacgc gaagcgagag tgagccgtaa aactctgagc tcctgtttta
      421 tagattacaa ctttaggccg tcttaaagct gaaagatttt cgaaagctat aaattgaagc
      481 ccttccacag tacatagatc tgtgttgtgg cggggcttta ccacgctgat tgccggagaa
      541 gaactcaacc tgctggcaaa acaaggcatg agatctttgc aataacatga gttgagacct
      601 ttgcaaaaaa g
//
LOCUS       DAWFJW010000249          604 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWFJW010000249 DAWFJW010000000
VERSION     DAWFJW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.249"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            114..604
                     /locus_tag="WGR19_002141"
                     /pseudo
     CDS             114..604
                     /locus_tag="WGR19_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          187 a          148 c          155 g          114 t
ORIGIN      
        1 ggtatccggg gaggattaag ggggtatttg ggtaaaatta ggcggtattt ggggcgaaaa
       61 cagccgaaaa cctgtgtttg ggtttcggct gtcgggaggg aagggaattt tgcaaaggtc
      121 tccaattgaa tgttcagaaa agtacccgtt tcatcggcat caaagtgggt aaaagcaatt
      181 acagcaaaaa cgagctgaac gaaaccaaac tgcccgtacg cgttatcgcc caaacagcca
      241 aaacccgttc aggctgggat accgtgctcg aaggtaccga attcaaaacc acgctggccg
      301 gtgccgacat tcaggcaggt gtaggcgaaa aagcccgtgt cgatgcgaaa attatcctca
      361 aaggcattgt gaaccgtatc cagtcggaag aaaaattaga aaccaactca accgtatggc
      421 agaaacaggc cggacgcggc agcactatcg aaacgctaaa actgcccagc ttcgaaagcc
      481 ctactccgcc caaattgtcc gcacccggcg gctatatcgt cgacattccg aaaggcaatc
      541 tgaaaaccga aatcgaaaag ctgtccaaac agcccgagta tgcctatctg aaacagctcc
      601 aagt
//
LOCUS       DAWFJW010000250          600 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWFJW010000250 DAWFJW010000000
VERSION     DAWFJW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.250"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..300
                     /locus_tag="WGR19_002142"
     CDS             <1..300
                     /locus_tag="WGR19_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799581.1"
                     /translation="DFLFVLVNTVKAMIENTDKSQLSELDKILDGVTSQELKLAYDFC
                     QGKFGQAGFSYRRHPNYFYLSSLIATFPEFELSKADRDYLKGIINFDNYLLYELD"
     gene            344..>600
                     /locus_tag="WGR19_002143"
     CDS             344..>600
                     /locus_tag="WGR19_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0799582.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT          187 a           90 c          131 g          192 t
ORIGIN      
        1 gattttcttt ttgttttagt aaatacagtg aaagctatga tagaaaatac tgataaaagt
       61 cagctttcag agttagataa aattttagac ggtgtcacaa gtcaagaatt gaaacttgcc
      121 tatgattttt gtcaagggaa attcggtcaa gcaggttttt cttatcgtag gcacccgaat
      181 tatttttatt tgtccagttt aattgcgact tttccagaat ttgagttgtc taaagcagac
      241 cgagattacc tcaaaggaat cataaatttt gataactacc ttttatatga gttagattag
      301 tctttaatca cctcaaaacc ctatttattc aaaaggagaa aaaatgattg agctgaaatt
      361 agtagatgag agcagttttc aggcagtgct ggatttgaaa atatctgaag ctgatgaacg
      421 agcacgtttt gtggctccaa atgtacgctc tttagctgac gcatggctct accgggaaaa
      481 tgaagatgtg tttccgaggg caatctattg ggataagcaa gtggttggct ttctcctgct
      541 ggaaatagac aaggatgaag cagaatactt tatctggcgg ataatgattg gtcagcagtg
//
LOCUS       DAWFJW010000251          589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWFJW010000251 DAWFJW010000000
VERSION     DAWFJW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.251"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          172 a          164 c          120 g          133 t
ORIGIN      
        1 tgtactggtt tttgttaatc cactatatcc gtttgcgccc gccaagccgt gttgcacaga
       61 tgcgacacgg ctttttcctt tctgttgtcc aaatgtaaat tttcgtcaaa aaacatcaaa
      121 aattaccccc cccgaatttg cgtaaaataa agtgtaatcc ctttgatcat aagggaatgt
      181 tgacaattag atcgattctc ctaggctgac tttcgcaata aatggggttg tcaataattg
      241 tgtatggtcg tttgccgtcc ggcggccgtc attcccgcga acgcaggaat ccagcaacac
      301 aacgcagcag gactttatcg gaaaaaaccg aaatcccacc gccgtcattc ccgcgcaggc
      361 gggaatccaa agacgcaacg caacaggaat ttatcggaaa aaacagaacc cccccaccgc
      421 cgtcattccc gcaaaagcgg gaatctagaa acacaacgcg gcaggacttt atcagaaaaa
      481 acagaaaccc caccgccgtc attcccgcaa aagcgggaat ctagacccgt cggcacggaa
      541 tcttaccgga taaaacagtt tccttagatt ccacgtccta gattcccgc
//
LOCUS       DAWFJW010000252          580 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWFJW010000252 DAWFJW010000000
VERSION     DAWFJW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.252"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            189..>580
                     /locus_tag="WGR19_002144"
     CDS             189..>580
                     /locus_tag="WGR19_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ0799583.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQQYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTSTA"
BASE COUNT          165 a          130 c          163 g          122 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg cggaactggc ggatttgaaa ccgctctaca
      181 aagactttat gtattgggaa cgcggtctgc acatgtataa ggcaagtgcc gtcgtgccga
      241 cgggatatgt acgggttggg aataccgcgc cgctggtcgg cgaagacacg cagcagtatg
      301 cctctttttg gggcgacggc tacgacgtgt accgtcagtt gagatggcag cagatacccg
      361 aaaaacagag aaaggcattc aaaaaagccg ccaaaagcaa aaagaccgtg atgtttgccg
      421 gacgggaata cggcatatcc aaacagaatt tgagcgatgt ttgggacgat tttgaagacg
      481 cgatggaact gaaggcgttt ccctgcctgt cttcgctgtt tctgaccaaa tggcataaaa
      541 atctatatga tagtggatta acaaaaacca gtacagcgtt
//
LOCUS       DAWFJW010000253          566 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWFJW010000253 DAWFJW010000000
VERSION     DAWFJW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.253"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>566
                     /locus_tag="WGR19_002145"
     CDS             <1..>566
                     /locus_tag="WGR19_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799584.1"
                     /translation="NPRQTHQFAQSQPLTKTDAKDAKMPAFFTQMMTQKEDWQTMPYQ
                     PPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQ
                     IDNHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRES
                     GETKFKSRCFGGKSAVRKALYMATVAAT"
BASE COUNT          162 a          161 c          151 g           92 t
ORIGIN      
        1 ccaacccgcg tcagacgcat cagtttgccc aatcgcagcc gctgaccaaa accgacgcca
       61 aagatgccaa aatgcccgcc ttcttcacac agatgatgac gcagaaagaa gattggcaaa
      121 ccatgcccta ccaaccgccc accgaagcgg aagaagtgtt ggaagccttg gttaaccgcc
      181 gcaaccaatc ggcggatatg cggactgccg agaaaaaccg tctgcatcag gttcatgaaa
      241 cgcaagtcgg aagcgtcaaa caactgattg cccattttga ccggctgatt gacgaattgg
      301 acaaacaaat cgacaaccac acccacacgc attttgacgg caaagcccaa gtggcagagc
      361 aaatcaaagg catcggttcg ataacgacgg ctacgctgat ggcgatgctg cccgaattgg
      421 ggcggctgtc gcacaaacgg atagcgagtt tggccggcat tgccccgcac ccgagggaga
      481 gcggggaaac caaattcaaa agccgctgct ttggcggaaa gtctgcggta cgtaaggcgc
      541 tgtatatggc taccgtggca gcgaca
//
LOCUS       DAWFJW010000254          564 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWFJW010000254 DAWFJW010000000
VERSION     DAWFJW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.254"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..314)
                     /locus_tag="WGR19_002146"
     CDS             complement(<1..314)
                     /locus_tag="WGR19_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799585.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIR"
BASE COUNT          141 a          140 c          139 g          144 t
ORIGIN      
        1 cggatagtat attcccaaaa gcgtttttgc caaagcctga gattgccgcc gattaaatat
       61 tggctgtgcc gcttgatttg ccgccagcgt tccgaataag cagaatcatt gtccggcagc
      121 cgccatatgg tatgcagatg gtcgggcatc aacacccatg ccaaaatttc aaacggatac
      181 cgttcgcgca ccgccattac cgcctgccgt aaagccaaac gcaccgcatc atcggtcaaa
      241 atcttctgcc gtttattggt tacaaccgta aaaaagtaag tgccgccatt gcggtaaaaa
      301 cgacggtatt tcatagtatt atgctcggaa tgattttgta ggtcggattc ttgaattcga
      361 cattttgggc attgctgcaa tggattgcaa tgatgggaat gttaaaggtt ttgtcggata
      421 caagtatccg acctacgctt gctgaaccgt cattcccacg gaagtgggaa tctagaacgc
      481 ggggtttcag tcatttccga tagattcccg ccgcgtcagg gggctggatt cccgcctgcg
      541 cgggaatgac gaatccatcc gcac
//
LOCUS       DAWFJW010000255          555 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWFJW010000255 DAWFJW010000000
VERSION     DAWFJW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.255"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            81..236
                     /locus_tag="WGR19_002147"
     CDS             81..236
                     /locus_tag="WGR19_002147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799586.1"
                     /translation="MEVTRNLKQAKPNELDSHFRGNDGMQVRGNDGMQVRGDDEFQVS
                     VFGFLFL"
BASE COUNT          160 a          102 c          151 g          142 t
ORIGIN      
        1 agacattcaa tactaaggca atttatcgga aatgactgaa actcaagaaa ctggattccc
       61 gcctgcgcgg gaatgacgaa gtggaagtta cccgaaattt aaaacaagcg aaaccgaacg
      121 aactggattc ccactttcgt gggaatgacg ggatgcaggt gcgtgggaat gacgggatgc
      181 aggttcgtgg ggatgacgaa tttcaggttt ctgtttttgg ttttctgttt ttgtgaaaat
      241 aacgggattt tagcttgtgg gtatttactg gaaaaaacag aaaccgctcc gccgtcattc
      301 ccgcgcaggc gggaatctag acattcaatg ctaaggcaat ttattgggaa tgactgaaac
      361 tcaaaaaact ggattcccac tttcgtggga atgacgggat gtaggttcgt gggaatgacg
      421 ggatataggt ttccgtatgg atggattcgt cattcccgcg caggcgggaa tctagacatt
      481 caatgctaag gcaatttatc gggaatgact gaaactcaaa aaactggatt cccactttcg
      541 tgggaatgac gaagt
//
LOCUS       DAWFJW010000256          555 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWFJW010000256 DAWFJW010000000
VERSION     DAWFJW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.256"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(262..>555)
                     /locus_tag="WGR19_002148"
     CDS             complement(262..>555)
                     /locus_tag="WGR19_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0799587.1"
                     /translation="ARIAYGHVKHQIHSVESKTTTVTSNNGGPIPQGPTPKPPYHESN
                     SISSLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          123 a          112 c          179 g          141 t
ORIGIN      
        1 cctgcgcagg aatgacgggt ttcgagattg cgttgttgtc gggaatgcaa ctgaaccgtc
       61 attcccacgg aagtgggaat ctaggacgta aaatctaaag aaaccgtttt atccgataag
      121 tttctgtgcg gacaggtctg gattcccgcc tgcgcgggaa tgacgaattt cgagattacg
      181 gtgtatcggg aatgatggga aacggtggga attgtgtaaa aaatgccgtc tgaaggttca
      241 gacggcatcg gtgtcgggga atcagaagcg gtagcgcacg cccaatgaga cttcgtgggt
      301 tttgaagcgg gtgttttcca agcgtcccca gttgtggtag cggtatccgg tgtccaaggt
      361 cagcttgggt gtgatgtcga aaccgacacc ggcgacgaca ccaagaccta agctgctgat
      421 gctgttgctt tcgtgatagg gaggtttggg ggtcggacct tgtgggatag gacctccgtt
      481 gttagaggta acagtcgtgg ttttgctttc cactgaatga atctgatgtt taacgtgtcc
      541 gtaggcgatg cgcgc
//
LOCUS       DAWFJW010000257          551 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWFJW010000257 DAWFJW010000000
VERSION     DAWFJW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.257"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..>551)
                     /locus_tag="WGR19_002149"
     CDS             complement(<1..>551)
                     /locus_tag="WGR19_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0799588.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRHTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHE"
BASE COUNT          176 a           88 c           93 g          194 t
ORIGIN      
        1 tattcgtgag tgcctgcaaa agcatattca tcattattac cttttaaatt cataatagtg
       61 ggtaaatttc cgataaaatt ttcaaatcca aaaatttcat ctaacaaaat ctttaaataa
      121 gcatttccgt catcatcaat ggaaataaca ataatcccat catcagttaa taattctttg
      181 gcaattgata aacgattttt cataaatgta agccaagtag ataatttaaa gttagtgtta
      241 taaccaaaac tatcttgttg tttcttttta acaaaataat aaggcggatc aatataaatc
      301 aacttcactt tgcctttaaa ctgtttggca agcgaatgta gggctatcag attattgcct
      361 ttgataatga gattttcggc gggtgtgccg tctgaatgtc gtttgatttc gccaaccgct
      421 tgtttgccgt ctgcggtgtg gcgagagaat tttgagaatg cttttgcgtc aaaaagccgg
      481 tcaatttcat caaaagcaag cgtttgatta aaaaagattt cttgtctttt tcgggttaat
      541 ttagtgtata a
//
LOCUS       DAWFJW010000258          507 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWFJW010000258 DAWFJW010000000
VERSION     DAWFJW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.258"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>507
                     /locus_tag="WGR19_002150"
     CDS             <1..>507
                     /locus_tag="WGR19_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0799589.1"
                     /translation="TRYLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSPITRID
                     FNLFCRFDELSIPDYSTLCRYRNWLAQDDTLSELLKLINRQLTEKGLKIEKASAAVVD
                     ATIIQTAGSKQRQAIEVDEEGQVSGQTTPSKDKDARWTKKNSLYKLGYKQHTRTDAEG
                     YIEKLYITP"
BASE COUNT          147 a          166 c          105 g           89 t
ORIGIN      
        1 acccgttacc ttagagacca ccgcggccgt cccgcctacc ccctgttgtc catgttcaaa
       61 gccgtcctgc tcggacaatg gcacagcctc tccgatcccg aactcgaaca cagccccatc
      121 acccgcatcg acttcaacct gttttgccgt ttcgacgaac tgagcatccc cgattacagc
      181 accttatgcc gctaccgcaa ctggctggcg caagacgaca ccctgtccga attgctcaaa
      241 ctgattaacc gccaactgac cgaaaaaggt ctaaaaatag agaaagcatc cgccgccgtt
      301 gttgacgcca ccattattca gaccgccggc agcaaacagc gtcaggctat agaagtcgat
      361 gaagaaggac aagtcagcgg ccaaaccaca ccgagtaagg acaaagatgc ccgttggaca
      421 aagaaaaaca gcctctacaa actcggttac aaacaacata cccgtaccga tgcggaaggc
      481 tatatcgaga aactgtacat cactccc
//
LOCUS       DAWFJW010000259          498 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWFJW010000259 DAWFJW010000000
VERSION     DAWFJW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.259"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          155 a          139 c           87 g          117 t
ORIGIN      
        1 tatcccgtca ttcctacgaa cctacatccc gtcattccct caaaaacaga aaaccaaaat
       61 tagaaaccta aaatcccgtc attcccgcgc aggcgggaat ccaaacttgt ccgcacggaa
      121 aacttatcgg ataaaacggt ttcttagatt ccacgttcta gattcccgcc tgcgcgggaa
      181 tgacgaatcc atccgcacgg aaacctatat cccgtcattc ttacgaacct acatcccgtc
      241 attccctcaa aaacagaaaa ccaaaattag aaacctaaaa tcccgtcatt cccacgaaag
      301 tgggaatcta gttcgttgag tttcagttat ttagaataaa ttttgaaact ttaatcccgt
      361 cattcccgca aaagcgggaa tccagaacgt aaaatctaaa gaaaccgttt tacccgataa
      421 gtttccgcac cgacagacct agattcccgc ctgcgcggga atgacggctg cagatgcccg
      481 acggtcttta tagtggat
//
LOCUS       DAWFJW010000260          494 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWFJW010000260 DAWFJW010000000
VERSION     DAWFJW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.260"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          140 a          100 c          124 g          130 t
ORIGIN      
        1 actcaacgca ctggattccc gcctgcgcgg gaatgacgaa gtagaagtta cccgaaattt
       61 aaaacaagcg aaaccgaacg aactggattc ccgcttgcgc gggaatgacg aatttcaggt
      121 ttctgttttt ggttttctgt ttttgtgaaa ataacaggat tttagcttgt gggtatttac
      181 tggaaaaaac agaaaccgct ccgccgtcat tcccgggcag gcgggaatct agacattcaa
      241 tactaaggca atttatcggg aatgactgaa actcaaaaaa ctagattccc actttcgtgg
      301 gaatgacgga atgtaggttc gtgggaatga cgtggtgcag gtttccgtat agatggattc
      361 gtcattctcg cgcaggcggg aatccagaca ttcaatgcta aggcaattta tcggaaatga
      421 ctgaaactca aaaaactgga ttcccacttt cgtgggaatg acggcggagc ggtttctgct
      481 ttttccaata aatg
//
LOCUS       DAWFJW010000261          476 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWFJW010000261 DAWFJW010000000
VERSION     DAWFJW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.261"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..410)
                     /locus_tag="WGR19_002151"
     CDS             complement(<1..410)
                     /locus_tag="WGR19_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799590.1"
                     /translation="MKADIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPAI
                     IGLLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSSEEGEEIYFKHS"
BASE COUNT          133 a          107 c           81 g          155 t
ORIGIN      
        1 ttactgtgtt taaaataaat ctcttcgcct tcttcgctgc tttgcccgcc attcaatacg
       61 caatctttaa acggaaaatc caacacaata tccgaactgt ctttcaaaaa gcggttgccg
      121 tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat tattgacgct gtgtttgtcc
      181 aagaaaaaac gaaaatcctg caatttaaac accaaaacac cattcacttc cacaaaaaaa
      241 tggcgtttta actcatcatt ccccaataac aaaccgataa tggccgggtc ggttttttcc
      301 accaaatcca acagaatgtt tttggctaaa atggtttttt cttcgttcgc ccatactttt
      361 tcgtgtgata gcagggcttg ggttaattcg gtttgaatgt ctgccttcat cgttataaat
      421 cctcatcagt cattttaatg tgccattctt gtgtaacggc aaatggtttt gcaaat
//
LOCUS       DAWFJW010000262          458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWFJW010000262 DAWFJW010000000
VERSION     DAWFJW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.262"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            124..458
                     /locus_tag="WGR19_002152"
                     /pseudo
     CDS             124..458
                     /locus_tag="WGR19_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          136 a          131 c          109 g           82 t
ORIGIN      
        1 tttgaaactt taatcccgtc attcccgcga aagcgggaat ccagaacgca aaatctcaag
       61 aaactgtttt cccgataagt ttccgtgccg acagacctag attcccgcct gcgcgggaat
      121 gacgaagcca ttcttttggc tgaaggtcaa aaatcagccg tcaccgagta ttacctgaat
      181 cacggcgaat ggcccgccaa caacagttct gccggcgtgg catcctcctc tgaaatcaaa
      241 ggcaaatatg ttgagaaagt tgaagtcgca aacggcgtca ttaccgccga aatgaaatca
      301 agcggcgtaa acaacgaaat caaaggcaaa aaactctccc tgtgggccaa gcgtcaagac
      361 ggttcggtaa aatggttctg cggacagccg gttacgcgcg acggcaccgc cggcaccaac
      421 aacgacgaag tcaaagccgc caccggcacc accgccaa
//
LOCUS       DAWFJW010000263          448 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWFJW010000263 DAWFJW010000000
VERSION     DAWFJW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.263"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          125 a           72 c           98 g          153 t
ORIGIN      
        1 aagtgttgtt gcaacagcta acgccatggc agccggtttg aaagtttttg tagggaaggt
       61 tggggtcgtt cgcatgatga ggtctccttg aatctgacaa tagaattcag gcgagggaat
      121 aaaaattccg taatgtttag atgtaacccg tttttatatt ttgaattata ttattatagg
      181 ggttgtcttg gacaactagg ataaactcga ttttactaat tgttttaaaa tggaaatttg
      241 aactttcgac aaatagctca atgagtttat tttgtttata ccggcttaga cggctttttc
      301 tcatagggat aattctaact taatttgaat ttccctagtt atagtggatg aacaaaaacc
      361 agtacggcgt tgcctcgcct tagctcaaag agaacgattc tttaacaagt gaatcggttc
      421 cgtactatct gtactgtctg cggcttcg
//
LOCUS       DAWFJW010000264          445 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWFJW010000264 DAWFJW010000000
VERSION     DAWFJW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.264"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..279
                     /locus_tag="WGR19_002153"
     CDS             <1..279
                     /locus_tag="WGR19_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799591.1"
                     /translation="ADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIKQVANNL
                     PDGSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVILPVKVPSKTNIRR"
     gene            282..>445
                     /locus_tag="WGR19_002154"
     CDS             282..>445
                     /locus_tag="WGR19_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0799592.1"
                     /translation="MGHNMMTTQKWYEHITNVIMGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYT"
BASE COUNT          148 a           73 c          108 g          116 t
ORIGIN      
        1 gccgatggta gtgttgtttt gattgtagaa agtaagcaga ttaggaacgg tacggtacag
       61 ctgaatccga atggtgcggg tggatatact cagatgagtg aggattggat taaacaagtt
      121 gcaaataact tacctgatgg tagtcctgct aaagctgctg tcttcaaagc ggagcgggaa
      181 ggcaaattaa aaacagcaat agcaggcgtt gatcgtcaaa caggtaaggc cgttattctt
      241 cctgtcaaag ttccttctaa aaccaatata aggagataac aatggggcac aatatgatga
      301 ccacccaaaa atggtatgag catattacta atgtaatcat gggcaatact gctaatttta
      361 atagcggttg ccctgaagct atagattatg tagatgaaag aaaaggcgtt ccacttgcag
      421 caatgagaca tattttaatg tacac
//
LOCUS       DAWFJW010000265          422 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWFJW010000265 DAWFJW010000000
VERSION     DAWFJW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.265"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>422
                     /locus_tag="WGR19_002155"
     CDS             <1..>422
                     /locus_tag="WGR19_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0799593.1"
                     /translation="NNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILA
                     ALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGE
                     ALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDV"
BASE COUNT          118 a           97 c          128 g           79 t
ORIGIN      
        1 tgaacaatgt cagcgataag cagtggatca acaacctgac cgtcaaccta gccaatgcgg
       61 gcagtgccgc actgattaat accgctgtca acggcggcag cctgaaagac aatctggaag
      121 cgaatatcct tgcggctttg gtcaataccg cgcatggaga agcagccagt aaaatcaaac
      181 agttggatca gcactacata gtccacaaga ttgcccatgc catagcgggc tgtgcggcag
      241 cggcggcgaa taagggcaag tgtcaggatg gtgcgatagg tgcggctgtg ggcgagatag
      301 tcggggaggc tttgacaaac ggcaaaaatc ctgacacttt gacagctaaa gaacgcgaac
      361 agattttggc atacagcaaa ctggttgccg gtacggtaag cggtgtggtc ggcggcgatg
      421 ta
//
LOCUS       DAWFJW010000266          415 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWFJW010000266 DAWFJW010000000
VERSION     DAWFJW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.266"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT           93 a           80 c          127 g          115 t
ORIGIN      
        1 tttgaggaat gacgggatgc aggttttctt aaccctgcgt cctagattcc cgctttcgtg
       61 gtaatgacgg gatgtgggtt cgtgggaatc tagaaatgaa aagcaacagg catttatcgg
      121 aaatgaccga aactgaacgg actggattcc cgcttttgcg ggaatgacgg ttcagttgct
      181 acggctactg tcaggtttcg gttatgttgg aatttcggga aacttatgaa ttgtcattcc
      241 cgcgcaggcg ggaatctaga ccggtcggtg cggaaactta tcgggaaaaa aggtttcttg
      301 agattttacg tcctggattc ccactttcgt gggaatgacg tggtgcaggt tcgtaggaat
      361 gacgtggtgc aggtttccgt acggatggat tcgtcattcc cgcgcaggcg ggaat
//
LOCUS       DAWFJW010000267          412 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWFJW010000267 DAWFJW010000000
VERSION     DAWFJW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.267"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          117 a          103 c           99 g           93 t
ORIGIN      
        1 tgcgcgggaa tgacaactca ttagttacct aaaacttaaa aagcagaaac ctttacgccg
       61 tcattcccac gaaagtggga atccgggaac ttaacgttac agtgatttat cggaaacggc
      121 tgaaaccgaa tgaattggat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa
      181 cctgcaccac gtcattccca cgaacctaca ttccgtcatt cccacgaaag tgggaatcca
      241 ggacgaaaaa tctccagaaa ccgttttacc cgataagttt ccgcaccgac aggtctggat
      301 tcccgcctgc gcgggaatga cgatgcaggt atttctgacg attcccggct atgatgttga
      361 ggcagaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cg
//
LOCUS       DAWFJW010000268          402 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWFJW010000268 DAWFJW010000000
VERSION     DAWFJW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.268"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          109 a          107 c           87 g           99 t
ORIGIN      
        1 gaaagtggga atctagaaat ttaatgttgc ggcactagcc aaaaaaaccg aaaccgacag
       61 gtctagattc ccgcctgcgc gggaatgacg aatccattcg tacggaaacc tgcaccacgt
      121 cattcccacg aacctgcatc ccgtcattcc cacgaaagtg ggaatccagt tttttgagtt
      181 tcagtcattt ccgataaatt gccttagcat tgaatgtcta gattcccgcc tgcgcgggaa
      241 tgacgcatcc atccgtacgg aaacctgcac cacgtcattc ccacgaaagt gggaatccag
      301 ttttttgagt ttcagtcatt ttcaataaat tgccttagca ttgaatgtct agattcccgc
      361 ctgcggggga atgacgaatc catccgtacg gaaacctgca cc
//
LOCUS       DAWFJW010000269          396 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWFJW010000269 DAWFJW010000000
VERSION     DAWFJW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.269"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          146 a           59 c           64 g          127 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtca
//
LOCUS       DAWFJW010000270          386 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWFJW010000270 DAWFJW010000000
VERSION     DAWFJW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.270"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          148 a           32 c           76 g          130 t
ORIGIN      
        1 ctagattccc actttcgtgg gaatgacggg atgtgggttt ttgtgcggat ttgaaccagt
       61 aagggtggtg tgggattggt ggtttgctta ggatcttttg gattgtattt tgtatataca
      121 tttacttgtt gataaaagat aaaataaaat tagaaactaa aagtgagaaa aaattaataa
      181 taatagggat gtataaatgt aaagactccg tttcatagct aaggttatct gaatatatgg
      241 aaaaaaagta aaagtccata aaactaaaat atatagataa tgctaatgat aatagaatta
      301 tcactttcct agagtaagga taatattttt ttatcgtaaa aaaatataaa ataggataaa
      361 taattgccat atagatagat tttgta
//
LOCUS       DAWFJW010000271          385 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWFJW010000271 DAWFJW010000000
VERSION     DAWFJW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.271"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>385
                     /locus_tag="WGR19_002156"
     CDS             <1..>385
                     /locus_tag="WGR19_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0799594.1"
                     /translation="SRFCGNDESILLAEGQKSAVTEYYLNHGIWPGGNSSAGVATSSE
                     IKGKYVKSVEVKNGVVTATMASSNVNNEIKGKKLSLWAKRQDGSVKWFCGQPVARAAN
                     NTADDAVKADTAANDKKIDTAANDK"
BASE COUNT          116 a          115 c           92 g           62 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacgaatcca tccttttagc cgaaggtcaa aaatcagccg
       61 tcaccgagta ttacctgaat cacggcatat ggcccggcgg caacagttct gccggcgtgg
      121 caacctcctc tgaaatcaaa ggcaaatatg ttaaaagcgt tgaagtcaaa aacggcgtcg
      181 ttaccgccac aatggcttca agcaacgtaa acaacgaaat caaaggcaaa aaactctccc
      241 tgtgggccaa gcgtcaagac ggttcggtaa aatggttctg cggacagccg gttgcgcgcg
      301 ccgccaacaa caccgccgac gacgccgtca aagccgacac cgccgccaac gacaagaaga
      361 tcgacaccgc cgccaacgac aagaa
//
LOCUS       DAWFJW010000272          377 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWFJW010000272 DAWFJW010000000
VERSION     DAWFJW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.272"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT          120 a           67 c           74 g          116 t
ORIGIN      
        1 aagagatgaa acagcgtatc ggtcccctac cggttgaatt ttccgaaaaa aaagcgacgt
       61 aaccagcatc aacatatata agaacagcac aaatagcatc aatacatcac gcaacgaaaa
      121 aagtcagaaa aatgcactta gtagtgtttg gatatctttt gttttgtgct gttagtgtcg
      181 cttctttctg tgtttacagt ttagcagttg tacagtttta cagtaaggtt taaacaatga
      241 ctgatttatt tttaaatgca gatattgtag aggataaaaa tgaccaaagt cctttcagta
      301 acatttttga ttttttagcg agccttttca tttccccggc gagatcggaa atggcagcgg
      361 tactttggcc accgata
//
LOCUS       DAWFJW010000273          370 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWFJW010000273 DAWFJW010000000
VERSION     DAWFJW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.273"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT           76 a           81 c          109 g          104 t
ORIGIN      
        1 cggctcgatg aatcgtcaga aatacccgaa ccgtcattcc cgcgcaggcg ggaatctaga
       61 ccggtcggtg cggaaactta tcgggaaaaa aggtttctcc gtccctgaat cctggattcc
      121 cactttcgtg ggaatgacag aatgtaggtt cgtgggaatg acgtggtgca ggtttccgtg
      181 cggatgaatt catcattccc gcgcaggcgg gaatctagtc tgttcggttt cagttatttt
      241 cgataaatgc ttgttgcttt tcatttctag attcccactt tcgtgggaat gacgggatgt
      301 aggttcgtag gaatgacgtg gtgcaggttt ccgtgcggat ggattcgtca ttcctgcgca
      361 ggcgggaatc
//
LOCUS       DAWFJW010000274          369 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWFJW010000274 DAWFJW010000000
VERSION     DAWFJW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.274"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT           97 a           76 c           90 g          106 t
ORIGIN      
        1 ggtccgttcg gtacggaaac ttatcggata aaacggtttc ttcagatttt acgttctgga
       61 ttcccacttt cgtgggaatg acggaaagtg gcgggaatga cggttctggc attccttaaa
      121 ttacccgtgt atcgctgtaa atcttagaga tggcggaata tagcggatta acaaaaacca
      181 gtacggcgtt gtctcgcctt agctcaaaga gaacgattct ctgttgctac ggttactgtc
      241 aggtttcggt tatgttggaa tttcgggaaa cttatgaatc gtcattcccg cgcacgcggg
      301 aatctagacc ttagaacaac agcaatattc aaagattatc tgaaagtccg agattcagat
      361 tcccgcgtt
//
LOCUS       DAWFJW010000275          343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWFJW010000275 DAWFJW010000000
VERSION     DAWFJW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.275"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..226
                     /locus_tag="WGR19_002157"
     CDS             <1..226
                     /locus_tag="WGR19_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0799595.1"
                     /translation="GWYDFRCQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAKSE
                     QRLSVLSEWVGQLETEYSELNLNRYSVASP"
BASE COUNT           86 a           94 c           81 g           82 t
ORIGIN      
        1 cggctggtac gatttccgct gccaaaacgt cgtgtacgcg cccgaccgca gcccggaaat
       61 cagccgtatg ctttcttcga ttttggcagg ctacgcgtgg gataccgaaa acccgttcgt
      121 ggcgaaatcc gaacaacgcc tgtcggtctt atcagaatgg gtcggtcagt tggaaaccga
      181 atatagtgaa ttaaatttaa accggtacag cgttgcctcg ccttagctca aagagaacga
      241 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgaga cctttgcaaa
      301 attcctttcc ctcccgacag ccgaaacccc aacacaggtt ttc
//
LOCUS       DAWFJW010000276          335 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWFJW010000276 DAWFJW010000000
VERSION     DAWFJW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.276"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..>335)
                     /locus_tag="WGR19_002158"
     CDS             complement(<1..>335)
                     /locus_tag="WGR19_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0799596.1"
                     /translation="YLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEPFFFPCDMLNIQ
                     DPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQLERL
                     KQRSEKVLA"
BASE COUNT           90 a           74 c           77 g           94 t
ORIGIN      
        1 tgcgccaaga ctttctcgct acgttgtttc aaacgttcaa gctgcttgcc ttccaccatc
       61 agcagagtat tgtaaatcat atgccggttg aggtcgtagc ggtttacgaa ggcttctgta
      121 tcgttggcga tgttgtcgat attgcgcacc agaaactcac gcagctgcgg gctgtcgctc
      181 atcagcatca gaaacatcgg atcttgaatg ttgagcatgt cacagggaaa gaagaagggt
      241 tcagggtctg tagaatttac actccgcaaa atacccagct ttcctgcaac ataagcatat
      301 tgcttgaatt tattaagatc atgttcaaat agata
//
LOCUS       DAWFJW010000277          335 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWFJW010000277 DAWFJW010000000
VERSION     DAWFJW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.277"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..>335)
                     /locus_tag="WGR19_002159"
     CDS             complement(<1..>335)
                     /locus_tag="WGR19_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0799597.1"
                     /translation="YLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDMLNIQ
                     DPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQLDRL
                     KQRSEKVLA"
BASE COUNT           88 a           78 c           75 g           94 t
ORIGIN      
        1 tgcgccaaga ctttctcgct acgttgtttc aaccgatcaa gctgcttacc ctccaccatc
       61 agcagagtat tgtaaatcat atgccggttg aggtcgtagc ggtttataaa ggcttctgta
      121 tcgttggcga tgttgtcgat attgcgcacc agaaactcgc gcagctgcgg gctgtcgctc
      181 atcagcatca gaaacatcgg atcttgaatg ttgagcatgt cgcagggaaa gaagaagggt
      241 tcggggtctt catcatctac actctgcaaa atacccaact ttcctgcaac ataagcatat
      301 tgcttgaatt tcttaagatc atgttcaaat agata
//
LOCUS       DAWFJW010000278          304 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWFJW010000278 DAWFJW010000000
VERSION     DAWFJW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.278"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT           84 a           70 c           68 g           82 t
ORIGIN      
        1 ctgacaaata caaaaactgt gtacacaaga aagaccgccg aggcggtctt ttcggttatt
       61 gtcttcgctt aaaagccaga cttccgtcgc ggcctataaa cgcgggaaat ccgcgtgtat
      121 aggcatagtt ggcgaccaca agtgcaacct taagtgcatc tcccaacttt ggcagggtgt
      181 ggtatgcacg catgtattct atatcctgtc catcaaattt gggcaagact tcgctattga
      241 accgtttgat cttcctttcc taaaaaagcg cataaagagc aggtacttaa tatatatggc
      301 tgtg
//
LOCUS       DAWFJW010000279          289 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWFJW010000279 DAWFJW010000000
VERSION     DAWFJW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.279"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>289
                     /locus_tag="WGR19_002160"
     CDS             <1..>289
                     /locus_tag="WGR19_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799598.1"
                     /translation="FLGISYSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQ
                     FETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSKSTLWQKQAGR"
BASE COUNT           92 a           85 c           73 g           39 t
ORIGIN      
        1 tttctcggca tcagctacag caaagcacac gacaccacca cccaagtcat gaaaaccgcg
       61 ctgccctcaa gggtagttgc agaatcagcc aacctccaat cgggctggga taccaaactg
      121 caaggcacac agtttgaaac cacactgggt ggcgcaacca tacgcgcagg cgtaggcgag
      181 caggcacggg cagatgccaa gattatcctc gaagggatca aaagcagcat ccacacagaa
      241 accgtgagca gcagcaaatc tactctatgg caaaaacagg caggacggg
//
LOCUS       DAWFJW010000280          289 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWFJW010000280 DAWFJW010000000
VERSION     DAWFJW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.280"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT           88 a           76 c           48 g           77 t
ORIGIN      
        1 tttttttgaa tttcgggcaa cgcttaaatc ttcattccgc gcaggcggaa attatcggtg
       61 cggtacggca acttttttcg atatgaaaag accgtcattc ctgtaaaaac aaaaaatcaa
      121 aaacagaaaa ttgaaattcg tcattcccgc gcaggcggga atccaggacg taaaatctga
      181 gacctttgca aaattccttt ccctcccgac agccgaaacc caaacacagg ttttcagctg
      241 ttttcgcccc aaataccgcc taattttacc caaatatccc cttaatcct
//
LOCUS       DAWFJW010000281          279 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWFJW010000281 DAWFJW010000000
VERSION     DAWFJW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.281"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>279
                     /locus_tag="WGR19_002161"
     CDS             <1..>279
                     /locus_tag="WGR19_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ0799599.1"
                     /translation="YAAERITHDYPKATGANNTSTVSDYFRNIRTHSIHPRVSVGYDF
                     GGWRIAADYARYRKWNNNKYSVNTKKVQENQGTRKDLKTENQENGSFHA"
BASE COUNT           98 a           76 c           62 g           43 t
ORIGIN      
        1 tacgccgccg aacgtattac ccacgattat ccgaaagcaa ccggtgcaaa caacacaagc
       61 acagtaagcg attatttcag aaacatccgt acgcattcca tccacccccg ggtgtcggtc
      121 ggctacgact tcggcggctg gaggatagcg gcagattatg cccgttacag aaaatggaac
      181 aacaataaat attccgtcaa cacaaaaaag gtgcaagaaa accaaggcac cagaaaagac
      241 ctgaagacgg aaaatcagga aaacggcagc ttccacgcc
//
LOCUS       DAWFJW010000282          277 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWFJW010000282 DAWFJW010000000
VERSION     DAWFJW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.282"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>277
                     /locus_tag="WGR19_002162"
     CDS             <1..>277
                     /locus_tag="WGR19_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0799600.1"
                     /translation="TVKKYIDEVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHM
                     LSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRLPTFL"
BASE COUNT           79 a           65 c           71 g           62 t
ORIGIN      
        1 ggacagtgaa aaaatacata gacgaagtat taaccgctgg acacggcaaa ctctaccaaa
       61 gcgacggcag acacagcgtc aatccgactg agggctacgg cacaggcggc ttgttgcaag
      121 gcaaaaaaca tatgctttca ctgacttgga atgcgccgat tgaggcgttt acccgcgaag
      181 gcgatttctt tgaaggcaaa ggcgttgatg ttttgtatat gcacttccac aaagccaacg
      241 agtttttggg tatgacccgc ctgccgacat tcttatg
//
LOCUS       DAWFJW010000283          276 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWFJW010000283 DAWFJW010000000
VERSION     DAWFJW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.283"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT           68 a           83 c           64 g           61 t
ORIGIN      
        1 atttaattga attgcggaaa ttgacggatt cagtgagatt ggcgggatga agcctaccct
       61 atagcccgcc ttttatagcg gattaacaaa aaccggtacg ggttgccccg ccccggctca
      121 aagggaacga ttccctaagg cgcccaagca ccaggcgaac cggttccgta ccatttgtac
      181 tgcctgcggc ccgccgcctt gtcctgattt ttgttaatcc gctatacgaa cccgccccca
      241 tcccgaaaac gcaaacaata ccgtctgaaa gctctg
//
LOCUS       DAWFJW010000284          274 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWFJW010000284 DAWFJW010000000
VERSION     DAWFJW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.284"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            43..213
                     /locus_tag="WGR19_002163"
     CDS             43..213
                     /locus_tag="WGR19_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0799601.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     DKEDFYLLDFVI"
BASE COUNT           75 a           76 c           73 g           50 t
ORIGIN      
        1 cggtttttgt taacccacta tacaaaaaac aaggaaacac aaatggcgca atatatgtgc
       61 ggcccctgcg gctggattta cgatgaagaa ctcggcgacc cagaacacgg catcgccccc
      121 gggacaaagt ttgaagacat ccccgacgac tggaaatgcc ccgaatgcgg cgtggacaaa
      181 gaagatttct acctgttgga tttcgtgata tagcggcagc cgcaccctta gggaaaaccc
      241 cgtcatccgg cgttccgcct gcggcggcag ggaa
//
LOCUS       DAWFJW010000285          269 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWFJW010000285 DAWFJW010000000
VERSION     DAWFJW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.285"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..264
                     /locus_tag="WGR19_002164"
     CDS             <1..264
                     /locus_tag="WGR19_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ0799602.1"
                     /translation="FYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQT
                     DFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT           89 a           67 c           51 g           62 t
ORIGIN      
        1 ttctaccaac acaccaaaat aaccaaagca ttgaaagcgg agacttattt ttgtcgccct
       61 taccattctt gggagaaagg gctgaatgag aacaccaacg gactcatccg gcaatacttc
      121 cccaaacaaa ccgatttccg taacatcagt gatcgggaga tacgcagggt tcaagatgag
      181 ttgaaccacc gaccaagaaa aacacttggc tacgaaacgc caagtgtttt attcttgaat
      241 ctgttccaac cactaataca ctagtgttg
//
LOCUS       DAWFJW010000286          259 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWFJW010000286 DAWFJW010000000
VERSION     DAWFJW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.286"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            96..259
                     /locus_tag="WGR19_002165"
                     /pseudo
     CDS             96..259
                     /locus_tag="WGR19_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
BASE COUNT           60 a           65 c           73 g           61 t
ORIGIN      
        1 ttggagctat acttggaggt ggcgcaggag cttggtgggg atcattagga gctgaaaaag
       61 cttggagatg gtttgaggat ttcttaaatc ttaaccgcag cgacaaatac cacgtttacg
      121 accccctcgc cctagatcta gacggcgacg gcatagaaac cgttgccacc aaaggctttg
      181 caggtgcatt gttcgaccac cgcaatcaag gcatccgcac cgccaccggt tgggtttctg
      241 ccgatgacgg tctgcttgt
//
LOCUS       DAWFJW010000287          254 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWFJW010000287 DAWFJW010000000
VERSION     DAWFJW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.287"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>254
                     /locus_tag="WGR19_002166"
     CDS             <1..>254
                     /locus_tag="WGR19_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0799603.1"
                     /translation="NGDNIINAADAAFQSLRVWQDLNQDGISQANELRTLEELGIQSL
                     DLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLLAA"
BASE COUNT           80 a           61 c           56 g           57 t
ORIGIN      
        1 aaacggcgac aacatcatca acgcggcaga cgccgcattc caatccctgc gtgtatggca
       61 ggatctcaat caggacggca tttcccaagc taatgaattg cgtacccttg aagaattggg
      121 tattcaatct ttggatctcg cctataaaga tgtaaataaa aatctcggta acggtaacac
      181 tttggctcag caaggcagct ataccaaaac agacggtaca accgcaaaaa tgggggattt
      241 acttttagca gccg
//
LOCUS       DAWFJW010000288          250 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWFJW010000288 DAWFJW010000000
VERSION     DAWFJW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.288"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
     gene            <1..>250
                     /locus_tag="WGR19_002167"
     CDS             <1..>250
                     /locus_tag="WGR19_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0799604.1"
                     /translation="APGGYIVDIPKGNLKTQIEKLAKQPEYAYLKQLQTVKDVNWNQV
                     QLAYDKWDYKQEGLTGAGAAIIALAVAAVTGGVGAGLIG"
BASE COUNT           74 a           69 c           68 g           39 t
ORIGIN      
        1 ccgctcccgg cggctatatc gtcgacattc cgaaaggcaa tctgaaaacc caaatcgaaa
       61 agctggccaa acagcccgaa tatgcctatc tgaaacagct tcagacggtc aaggacgtga
      121 actggaacca agtacagctc gcttacgaca aatgggacta taaacaggaa ggcctaaccg
      181 gagccggagc cgcaattatc gccctggcag tagcggcggt aaccggagga gtaggcgcag
      241 gccttatcgg
//
LOCUS       DAWFJW010000289          245 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024756
            SAMN37720412-rid20221513.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWFJW010000289 DAWFJW010000000
VERSION     DAWFJW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720412
            Sequence Read Archive: SRR26318848
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985322.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 21:29:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,167
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 54 of 119
            Pseudo Genes (incomplete)         :: 66 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720412-rid20221513.denovo.289"
                     /strain="HB024756"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2019"
                     /collected_by="Suzhou CDC"
BASE COUNT           83 a           38 c           51 g           73 t
ORIGIN      
        1 aaaatcccct aaatgtcttg gtgggaattt aggggatttt ggggattttg caaaggtctc
       61 aacttacaaa ttggttttaa aacgatcaga ccaaattcat ctgccagcaa aaatggaatg
      121 ccgatatatt ctaacgtaag tcgaagagaa atatttgatt tgtatgaaca atattcaggg
      181 cagaggcctg attttaggaa tattccgaat aaagggcaat tatcttctac gactattaca
      241 tcagg
//
